LOCUS       DAFGZE010000001        89539 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.001, whole genome shotgun sequence.
ACCESSION   DAFGZE010000001 DAFGZE010000000
VERSION     DAFGZE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 89539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.001"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(3..1559)
                     /gene="rny"
                     /locus_tag="LTU54_000001"
     CDS             complement(3..1559)
                     /gene="rny"
                     /locus_tag="LTU54_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBL8294748.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(1970..3019)
                     /gene="recA"
                     /locus_tag="LTU54_000002"
     CDS             complement(1970..3019)
                     /gene="recA"
                     /locus_tag="LTU54_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBL8294749.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3128..4369)
                     /locus_tag="LTU54_000003"
     CDS             complement(3128..4369)
                     /locus_tag="LTU54_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBL8294750.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4609..5187)
                     /gene="pgsA"
                     /locus_tag="LTU54_000004"
     CDS             complement(4609..5187)
                     /gene="pgsA"
                     /locus_tag="LTU54_000004"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBL8294751.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5396..6319)
                     /locus_tag="LTU54_000005"
     CDS             complement(5396..6319)
                     /locus_tag="LTU54_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8294752.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7058..8344)
                     /locus_tag="LTU54_000006"
     CDS             complement(7058..8344)
                     /locus_tag="LTU54_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL8294753.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8337..9605)
                     /locus_tag="LTU54_000007"
     CDS             complement(8337..9605)
                     /locus_tag="LTU54_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBL8294754.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9857..10318
                     /gene="mscL"
                     /locus_tag="LTU54_000008"
     CDS             9857..10318
                     /gene="mscL"
                     /locus_tag="LTU54_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBL8294755.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10377..10823)
                     /locus_tag="LTU54_000009"
     CDS             complement(10377..10823)
                     /locus_tag="LTU54_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8294756.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10916..11161)
                     /locus_tag="LTU54_000010"
     CDS             complement(10916..11161)
                     /locus_tag="LTU54_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBL8294757.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11276..11854)
                     /locus_tag="LTU54_000011"
     CDS             complement(11276..11854)
                     /locus_tag="LTU54_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8294758.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(11931..12461)
                     /locus_tag="LTU54_000012"
     CDS             complement(11931..12461)
                     /locus_tag="LTU54_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBL8294759.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12465..13325)
                     /gene="rlmB"
                     /locus_tag="LTU54_000013"
     CDS             complement(12465..13325)
                     /gene="rlmB"
                     /locus_tag="LTU54_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBL8294760.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13318..13713)
                     /locus_tag="LTU54_000014"
     CDS             complement(13318..13713)
                     /locus_tag="LTU54_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBL8294761.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13710..15122)
                     /locus_tag="LTU54_000015"
     CDS             complement(13710..15122)
                     /locus_tag="LTU54_000015"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBL8294762.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15119..15655)
                     /gene="cysE"
                     /locus_tag="LTU54_000016"
     CDS             complement(15119..15655)
                     /gene="cysE"
                     /locus_tag="LTU54_000016"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBL8294763.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15741..15980)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(15977..17437)
                     /locus_tag="LTU54_000017"
     CDS             complement(15977..17437)
                     /locus_tag="LTU54_000017"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBL8294764.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17607..18764)
                     /locus_tag="LTU54_000018"
     CDS             complement(17607..18764)
                     /locus_tag="LTU54_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBL8294765.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18798..20168)
                     /gene="radA"
                     /locus_tag="LTU54_000019"
     CDS             complement(18798..20168)
                     /gene="radA"
                     /locus_tag="LTU54_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBL8294766.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20231..20758)
                     /locus_tag="LTU54_000020"
     CDS             complement(20231..20758)
                     /locus_tag="LTU54_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBL8294767.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            20988..21794
                     /gene="proB"
                     /locus_tag="LTU54_000021"
     CDS             20988..21794
                     /gene="proB"
                     /locus_tag="LTU54_000021"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBL8294768.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            21791..23038
                     /locus_tag="LTU54_000022"
     CDS             21791..23038
                     /locus_tag="LTU54_000022"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBL8294769.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            23114..23458
                     /locus_tag="LTU54_000023"
     CDS             23114..23458
                     /locus_tag="LTU54_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294770.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(23575..23901)
                     /locus_tag="LTU54_000024"
     CDS             complement(23575..23901)
                     /locus_tag="LTU54_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294771.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(24516..25718)
                     /locus_tag="LTU54_000025"
     CDS             complement(24516..25718)
                     /locus_tag="LTU54_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8294772.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            25835..26350
                     /locus_tag="LTU54_000026"
     CDS             25835..26350
                     /locus_tag="LTU54_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL8294773.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(26549..26995)
                     /locus_tag="LTU54_000027"
     CDS             complement(26549..26995)
                     /locus_tag="LTU54_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL8294774.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(27042..28520)
                     /gene="msr(C)"
                     /locus_tag="LTU54_000028"
     CDS             complement(27042..28520)
                     /gene="msr(C)"
                     /locus_tag="LTU54_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL8294775.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(28874..29503)
                     /locus_tag="LTU54_000029"
     CDS             complement(28874..29503)
                     /locus_tag="LTU54_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL8294776.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(29487..29951)
                     /locus_tag="LTU54_000030"
     CDS             complement(29487..29951)
                     /locus_tag="LTU54_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8294777.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(30438..31706)
                     /locus_tag="LTU54_000031"
     CDS             complement(30438..31706)
                     /locus_tag="LTU54_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL8294778.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(31937..32674)
                     /locus_tag="LTU54_000032"
     CDS             complement(31937..32674)
                     /locus_tag="LTU54_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8294779.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(32741..33310)
                     /locus_tag="LTU54_000033"
     CDS             complement(32741..33310)
                     /locus_tag="LTU54_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL8294780.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            33438..34310
                     /locus_tag="LTU54_000034"
     CDS             33438..34310
                     /locus_tag="LTU54_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8294781.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            34652..35524
                     /locus_tag="LTU54_000035"
     CDS             34652..35524
                     /locus_tag="LTU54_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8294782.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            35521..37479
                     /locus_tag="LTU54_000036"
     CDS             35521..37479
                     /locus_tag="LTU54_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8294783.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(37811..38662)
                     /locus_tag="LTU54_000037"
     CDS             complement(37811..38662)
                     /locus_tag="LTU54_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8294784.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            38785..39252
                     /locus_tag="LTU54_000038"
     CDS             38785..39252
                     /locus_tag="LTU54_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBL8294785.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(39515..39997)
                     /locus_tag="LTU54_000039"
     CDS             complement(39515..39997)
                     /locus_tag="LTU54_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL8294786.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(40097..40960)
                     /locus_tag="LTU54_000040"
     CDS             complement(40097..40960)
                     /locus_tag="LTU54_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL8294787.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            41558..42433
                     /locus_tag="LTU54_000041"
     CDS             41558..42433
                     /locus_tag="LTU54_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294788.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(42578..42730)
                     /locus_tag="LTU54_000042"
                     /pseudo
     CDS             complement(42578..42730)
                     /locus_tag="LTU54_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(42998..44329)
                     /locus_tag="LTU54_000043"
     CDS             complement(42998..44329)
                     /locus_tag="LTU54_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8294789.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(44478..45191)
                     /locus_tag="LTU54_000044"
     CDS             complement(44478..45191)
                     /locus_tag="LTU54_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8294790.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            45366..46790
                     /locus_tag="LTU54_000045"
     CDS             45366..46790
                     /locus_tag="LTU54_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8294791.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            47089..47796
                     /locus_tag="LTU54_000046"
     CDS             47089..47796
                     /locus_tag="LTU54_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL8294792.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            47783..48106
                     /locus_tag="LTU54_000047"
     CDS             47783..48106
                     /locus_tag="LTU54_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL8294793.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKIDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(48195..49232)
                     /locus_tag="LTU54_000048"
     CDS             complement(48195..49232)
                     /locus_tag="LTU54_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL8294794.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(49553..50536)
                     /locus_tag="LTU54_000049"
     CDS             complement(49553..50536)
                     /locus_tag="LTU54_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HBL8294795.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(50951..51721)
                     /locus_tag="LTU54_000050"
     CDS             complement(50951..51721)
                     /locus_tag="LTU54_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294796.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(51714..52649)
                     /locus_tag="LTU54_000051"
     CDS             complement(51714..52649)
                     /locus_tag="LTU54_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8294797.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(52965..54614)
                     /locus_tag="LTU54_000052"
     CDS             complement(52965..54614)
                     /locus_tag="LTU54_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL8294798.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(54731..57334)
                     /locus_tag="LTU54_000053"
     CDS             complement(54731..57334)
                     /locus_tag="LTU54_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL8294799.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(57346..58395)
                     /locus_tag="LTU54_000054"
     CDS             complement(57346..58395)
                     /locus_tag="LTU54_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBL8294800.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(58421..58735)
                     /locus_tag="LTU54_000055"
     CDS             complement(58421..58735)
                     /locus_tag="LTU54_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBL8294801.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(58751..59197)
                     /locus_tag="LTU54_000056"
     CDS             complement(58751..59197)
                     /locus_tag="LTU54_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8294802.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(59198..61120)
                     /locus_tag="LTU54_000057"
     CDS             complement(59198..61120)
                     /locus_tag="LTU54_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBL8294803.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(61332..62228)
                     /locus_tag="LTU54_000058"
     CDS             complement(61332..62228)
                     /locus_tag="LTU54_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8294804.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(62221..62901)
                     /locus_tag="LTU54_000059"
     CDS             complement(62221..62901)
                     /locus_tag="LTU54_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8294805.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(62902..63684)
                     /locus_tag="LTU54_000060"
     CDS             complement(62902..63684)
                     /locus_tag="LTU54_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294806.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(63677..64594)
                     /locus_tag="LTU54_000061"
     CDS             complement(63677..64594)
                     /locus_tag="LTU54_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8294807.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(64758..65081)
                     /locus_tag="LTU54_000062"
     CDS             complement(64758..65081)
                     /locus_tag="LTU54_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294808.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            65869..69096
                     /gene="fss3"
                     /locus_tag="LTU54_000063"
     CDS             65869..69096
                     /gene="fss3"
                     /locus_tag="LTU54_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL8294809.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            69209..69523
                     /locus_tag="LTU54_000064"
     CDS             69209..69523
                     /locus_tag="LTU54_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL8294810.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            69536..69910
                     /locus_tag="LTU54_000065"
     CDS             69536..69910
                     /locus_tag="LTU54_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL8294811.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            69920..70255
                     /locus_tag="LTU54_000066"
     CDS             69920..70255
                     /locus_tag="LTU54_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HBL8294812.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            70338..71678
                     /locus_tag="LTU54_000067"
     CDS             70338..71678
                     /locus_tag="LTU54_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL8294813.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            71756..72430
                     /locus_tag="LTU54_000068"
     CDS             71756..72430
                     /locus_tag="LTU54_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL8294814.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            72423..72587
                     /locus_tag="LTU54_000069"
     CDS             72423..72587
                     /locus_tag="LTU54_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294815.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            72663..73805
                     /gene="dcm"
                     /locus_tag="LTU54_000070"
                     /pseudo
     CDS             72663..73805
                     /gene="dcm"
                     /locus_tag="LTU54_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            73795..74085
                     /locus_tag="LTU54_000071"
     CDS             73795..74085
                     /locus_tag="LTU54_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294816.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            74344..75528
                     /locus_tag="LTU54_000072"
     CDS             74344..75528
                     /locus_tag="LTU54_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL8294817.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            75525..75662
                     /locus_tag="LTU54_000073"
     CDS             75525..75662
                     /locus_tag="LTU54_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL8294818.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            76408..78318
                     /gene="ltrA"
                     /locus_tag="LTU54_000074"
     CDS             76408..78318
                     /gene="ltrA"
                     /locus_tag="LTU54_000074"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL8294819.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            78422..78646
                     /locus_tag="LTU54_000075"
     CDS             78422..78646
                     /locus_tag="LTU54_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294820.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            78659..79162
                     /locus_tag="LTU54_000076"
     CDS             78659..79162
                     /locus_tag="LTU54_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL8294821.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            79222..79611
                     /locus_tag="LTU54_000077"
     CDS             79222..79611
                     /locus_tag="LTU54_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL8294822.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            79598..82045
                     /locus_tag="LTU54_000078"
     CDS             79598..82045
                     /locus_tag="LTU54_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8294823.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            82050..84173
                     /locus_tag="LTU54_000079"
     CDS             82050..84173
                     /locus_tag="LTU54_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL8294824.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            84170..85174
                     /locus_tag="LTU54_000080"
     CDS             84170..85174
                     /locus_tag="LTU54_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBL8294825.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            85193..86104
                     /locus_tag="LTU54_000081"
     CDS             85193..86104
                     /locus_tag="LTU54_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL8294826.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            86291..86547
                     /locus_tag="LTU54_000082"
                     /pseudo
     CDS             86291..86547
                     /locus_tag="LTU54_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(86764..86979)
                     /locus_tag="LTU54_000083"
     CDS             complement(86764..86979)
                     /locus_tag="LTU54_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294827.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(87228..87446)
                     /locus_tag="LTU54_000084"
                     /pseudo
     CDS             complement(87228..87446)
                     /locus_tag="LTU54_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(87741..89180)
                     /locus_tag="LTU54_000085"
     CDS             complement(87741..89180)
                     /locus_tag="LTU54_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8294828.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
BASE COUNT        26974 a        17860 c        16576 g        28129 t
ORIGIN      
        1 tattatttag cataatcagt tgcacgagtt tcccgaataa cgattacttt gatgtgtcca
       61 ggataatcca gttcttcttc gattcgttta cgaatatcac gtactaaacg aacagcgtct
      121 aaatccgaga tttcttctgg cttgaccatt acccgaactt cgcgtccagc ttgaactgcg
      181 taacttgatt ctacaccggc gaagctgttc gaaatatttt ccagacttct taaacgacgg
      241 atgtagtttt ctaatgattc acttcgcgca ccaggtcttg ctgctgagag cgcatctgct
      301 gcagctacaa ggacagagat aacagaagta gcttccacat ctccatgatg agaagcgatc
      361 gcgttgatta ctactgggtt ttctttgtac ttggcagcta attccgcacc gatctcaacg
      421 tgagatcctt caatctcatg gtcaagtgct ttcccaatat catggagcaa cccggcacgt
      481 tttgctaatt ggatatcttc gccaagctct gctgctaaga taccagctaa ttttgcaacc
      541 tcgaccgaat gcttcaagac gttttgtcca taactagttc tgaatcgcag acgtccgatg
      601 atcttgatta gatctggatg aagtgtatga gcaccgactt caaacgctgc ctcttccccg
      661 tattcgcgaa tacgttcatc catttcttta cgagatttct cgaccatttc ttcgatacgc
      721 gctggatgga ttcgtccatc ttgtatcagt ttttccaagg tcatccgtgc gatttctctt
      781 cggattggat cgaatccgga aagaacaact gcttccggtg tatcatcgat gattaaatcg
      841 ataccagtca gcgtctcaag cgttcgaata ttacgacctt ctcgtccaat gatacgacct
      901 ttcatttcat cattcggaag ggtaacgact gaaacagtcg tttctgaaac ttgatctgct
      961 gcgcagcgtt gaatcgctaa tgataataag tttttcgctt tgcgatctgc ttcttctttg
     1021 gcacgctgtt cagattcttt taccattaga gttaactcat gattcagctc ttcttcagtc
     1081 gatttcatga tgacatctcg tgcgtcctct tttgataaac cagcaatttt ttctagttct
     1141 ttttgttgat cttcaatcat tttttcaacg tctttttctc gctcttcaat taattgctgt
     1201 tttgaactaa gtgcttcttc tttactttca agtgagttct cgcgtttttc taacgagtca
     1261 tctttacgat tcagcgtttg ttcgcgttga atcaaacgat tctcttgaga ttttaattct
     1321 aacttacttt ctctcagctc actttcaact tcagaacggt atttctgatt ttcttcctta
     1381 gcttctaaca atgcttcttt ttttagagtt tctgcttctt tttttgcact atcgatgatt
     1441 ccagcagctg atgattgtgc accagctatt tctttctcgt gacgtgactt tgcgaccata
     1501 aaccctaaac caagaccgac aattaaaccg atgatagcga ggagaatgtt gaataccata
     1561 aaatccacct ccatactatc tttttcattt ttatcatcta tacagcttct atgataaatt
     1621 attctaaact cgtcaatatg catcttcgca tatatgtgta caaaaaacat gcacaacttt
     1681 attctaatgt ttgtaccccg tagtgtcaac tttaaataaa caaagaaacg gctttctact
     1741 tattcattaa acgatggttt ttaaaataaa tgacactagt ttcatatagg taaaccttta
     1801 aattgagaaa cttttctatt aaattattct ttgtagatga acagatattt ttattttatg
     1861 tacccactcg tataattaaa taactcacga tttctttatt caataaaaaa acggcaggtc
     1921 aaggagagta cctactcttc agaccagccg tttttgttta tcataatttt tattcttcta
     1981 ctaaaggcaa ttcttcttgt cctttttcat cttctgggac agcgacttct tcaccaatac
     2041 cataagctgc tctaactaga gcagaaactt ctgccatcat ttcaggatga ttagccatat
     2101 agattttagc gttttcccgt ccttgaccaa tccgatcttc tttataagaa taccaagcac
     2161 cgcttttatc aacaatgtct ttttctactg ccatgtctag aagttctcct tcttgtgaga
     2221 tccctaaacc atacatgaca tctacttcag cgattttgaa aggaggtgct actttgtttt
     2281 ttacgacttt gatctttgta cggttaccaa caatatctgt accttgtttc aattgttctg
     2341 cacgacgtac ttccaaacgg atcgttgcgt agaatttcaa tgcgcgtcct ccaggagtga
     2401 tttctggatt cccaaacatc acgcctactt tttcacggat ttggttgatg aaaatagcga
     2461 ttgtttttgt tttgttgatc gaaccagaga gtttacgcaa tgcttgagac atcaaacgtg
     2521 cttgtaaccc gacatgtgag tcacccattt cgccgtcgat ttctgctcgt ggaactaaag
     2581 cagcaactga atcgactact acgatatcta cagccccact tgatactaaa gcatcagcga
     2641 tctctagacc ttgttctcct gtgtcaggct gtgaaagaag tagttcatcg atattcacac
     2701 ctaatttttg tgcatattgc ggatctaacg catgctcagc atcaatgaaa gcggccgttc
     2761 cgccattttt ttgtacttct gcaatagcgt gtagtgcaac tgttgtttta cctgaacttt
     2821 caggtccata tacttcgatg atacgtccac gggggtaacc gccgacacct aaagcgacat
     2881 ctaacgcaag agagccactt ggaattgtag agatttgttg gtcgattttt tcgcccaatt
     2941 tcataataga gcctttacca tagctctttt caattttttt cagtgcagca tctaaggctg
     3001 ctttacgatc atctgccaat acgataatcc tccttatatt tacaaatcag agcaacattt
     3061 cattatcctt atcatatctt ttttagtgaa aaaaagcaag aaaaaaccga acaaatattc
     3121 gcatttatca ttttcttaaa agctgttttc tcaaaagatc aaatccttgc ataaccgcat
     3181 tatatctgat agcagcacga tctctggcaa agtgatattt ttccgaaacc acgccgtctt
     3241 tagatgaaat agcgatccat actgttcctg cagattgacc ttctaacgtg tccggtccag
     3301 ccactccggt gaaagctaca ctgtaatctg ttcttgctat ctcacgtgct tgaatggcca
     3361 tctgctccac acattctttg ctgactgttc catattctgc taatagctcc ggatcaatct
     3421 ccagaaaacc agcttttgtt tggagcgagt aggtaacgaa tccgcctgga aatacttcag
     3481 atacaccagc aatgtcgcct aatgcggctt ggaaggcgcc ggctgtcaaa ctttccgctg
     3541 ctgttacagt ctgcttgttt tctttcagta gttccactac gacttttgct agcgaattat
     3601 catcgccgta tccataaaaa tattccccta ctcgttcttg gattttttct tctaatgcta
     3661 aaagagcttg attccctgcc tgaacatcat ttgttttcac tgtcagtcgt aatgtcactt
     3721 catttggttt ggcatatggt gcgatcgttg gattgatctg tgtctcaatc agatctttta
     3781 gctcagtgac cagtcgcgat tcaccaatcc cataaaagcg caaaacttta gaaattagtt
     3841 tttcttcaga aggaaaacgt tcctctagta aaggacgtac ttgttccaca aacattggtt
     3901 tcaactcatt aggcggaccg ggaagaagga tatacgcatg tgtatctgtt tgataaaaag
     3961 tccctaaagc aagccctgta cgatttggta gaggaacccc gccttcgata atctgggact
     4021 gttgcagatt gtttttggtc atttttcgat gggttgtttc aaaatatgcc aataattttt
     4081 tgtatccttc cgtattttgt atgagagact taccgaggtg agccgcagtt gcttcttttg
     4141 tcaggtcatc ttctgtcggt cctaatccgc cacataagag tatgagttca cttcttgttt
     4201 cagctagagc aatcaactct tctaaacgct gctgattatc tcctacaact gtttgataat
     4261 aaacttcgat acctagatca gctagctgtt ctgacaagaa agtggcattc gtattcacga
     4321 cttgtcctag taaaagttct gttccgacag ctattatttc tgctttcatt acttcccgct
     4381 cccttatata gtatacgctg tatttttata tttttttatt taaaaagagg gcaaggagct
     4441 gtgacaaaaa tcttgtcaca gctccttgtt ccaaataaac agtgttctcc tgccaaaacc
     4501 tcggtcataa gtcacaatat acataaaaca tgaaaagctg ttttttgaaa ttatcccttg
     4561 tgactcagag ttagacggca ggctcacaac ctctccacta aacgatactt acattgatcc
     4621 tttgaacaca ccgctgtttt tgatgaagta atccacacct gaatagatag taaagatcag
     4681 acaagcataa agcatgattt gatctactgg aataccgatc aggctgaatg ggatattatt
     4741 gataaacagc aagataatag caaccatttg tgtagccgtc ttgacttttc ccggccaagc
     4801 tgcagccatg acttctccat gttcgacaag tagtaaacgc aagcctgtga cggctaattc
     4861 acgacagaca atgatcgcta ctacccaacc aggggccttt ccttggtcaa ccaacatgat
     4921 aaaagcagtc ataactagca ttttatctgc caacgggtcc gcaaacttgc caaaattagt
     4981 gacaaggcct cgcgcacggg caatttttcc atctaaccag tctgtgatac tagctaaagc
     5041 aaaaataact gcacctacta gctgtgtgac agccagtgtg gtgtctccta cgttaaattg
     5101 tccccaatct aaaggtaaac ctacaaccaa aatgaaaatc gggatcatac aaattctaag
     5161 tacagttaat ttatttggta aattcacact atcgactcct ttcttcccta ttctacgtga
     5221 ctatcagcga agtttccacc gatattattt acttttccaa atgaatttat tcaaaggagt
     5281 atttttattg aaattgatct ttttgaatta acgaagaagg accgcgacac agatttgtca
     5341 cgatccttct tttttatacc tacaagtaaa cagatgcgta aactaattta tacgatcatt
     5401 ctgcgtattg aagagtaagg ttcacatttt ttggactctg attgttttcc cctggattaa
     5461 ctggtacgga ttctccattc gctttgatcg ttgcattgct tgctgcacca atcgtgatgg
     5521 tcgcatttgt tgctgtttcc ggtaacacgg tactctgtgt ttcatttgct gccagtgtcc
     5581 cttgataaac ataggcattg tctactagca cacctaacca tacacgattg acagcagtaa
     5641 attcaagggt tacaggcttg gtagcgtctg tgatatttac tgcgatggca ctgccagaat
     5701 cttctcctgt agctattgcc attttctttt cttcacttga ttttgttgtt gattctactg
     5761 tagaagactc tttggtctgc gtgctttgct ctgaggaaga ggactcctcg actgatcctt
     5821 ctactttaag cgaagaagct gtgtctccga ttattggatc agcttgacga tcttgccaag
     5881 tcatatagcc cacgacaacg acaatcgcca aagcaaccaa tcctaataaa atgacaggaa
     5941 gacttgtcca aaagcgactt ggatgcgatt cttcctgatg cattgctttt ctagaagtac
     6001 ttactgtttc aggctgcgga cgttcaggga ccgatcctgt caatttgctt ttcccatcaa
     6061 aaacatcgac taaatggtct ccatcttctc ctacagcttc agcgtattgc cgaataaatg
     6121 cccgcacgta aaatttacca ggcaattggt caaaatcatc attgtcgatt gcttccaaat
     6181 aacgtttttg aatttttgta atctgctgta attcatctaa agatattttt ttattcaggc
     6241 gtgcttgccg taatttttct ccgatggttt caaaagccac tcgttcatct tctccagtct
     6301 gtttgattct ccgaagcatc tgctgttgca acgaaagacg ttatgtttgg gtgctgtgac
     6361 aaaagtcatg tcacagctcc ttttttccga ataaacattg ttctcctgcc aactcctcgg
     6421 tcacaagtca caatatccaa taaatataag aagctgtttt ttgaacttgt tccttgtgac
     6481 tcggagttaa acggcaggct cacgacctct ttcttaaacg gtgttcaatc agctgttcct
     6541 cggtattagc tcaaaaaccg gcaaaatata aggagatatt tttccgtatg tttctctaat
     6601 actcaggtca aaacgctgat ttcacaacct cttccttaaa cggtgttcaa aagcttgcat
     6661 ctgcggtaat aagtccaacc aagttaaaaa cattaagaag ctgtttttac ttggctgttc
     6721 ttattacttg atgcagggca gctttttcac aacctcttgg tacacggtgt tccagaagta
     6781 gcttcttcgg caataagccg aaatatcaca aaaatttgag aagcaatttt cgtaaattcc
     6841 ttcttatttc ttgaagctga ccacttctgt cacaacctct ttgattactt cgtttgattt
     6901 tattttaata taagttgatt aaaagagagc atgatttgtt ttattttctc actaattttt
     6961 ccaacacaac taagaatacc ataaaagctg attttggagg aggttcctac tacaagtcta
     7021 aaacgaaatt taagactttt cctcataaaa tactggttta ttttggtctc atgtaaaact
     7081 cgctgaaggc ctcttcactt ataaatgctt ccccagctgc cagtacgtct ttcatttgga
     7141 tcgaatcgat gatttcagga agatcaaata aagttcgatc gccaaataaa ctttgcgtaa
     7201 attgattggc aatatattcg atcgaattta atgattggaa atattgtcca agcattttct
     7261 tcttcagcaa atcgagattt gtttctgaca cttccggatc atcagcgaat ccaagaatga
     7321 tttctttgac tttttctgct gctttctctg gttcgtccgt atctccgctg aagtccgcaa
     7381 agtgaaattc acgatccaag ctgaattcaa aaccaaagct gtcatcgata attccttgat
     7441 tgtacatcgc tagatagttt cgagaagtat tgcctaaaat catttggaac aacagattga
     7501 ttgctgtttt atagcgtaac agttctgttc cttcttgcgg caatgtgtct aatcctttaa
     7561 taccaagtac aaatttatct cgtgtaatgg ctgcttccaa tgcactttgt ttgatgattt
     7621 cttttgtgtt ttcagggaaa taacgtacaa tttcttgctt tggagggaaa ttttttgctt
     7681 cttggttttc ccgaataagt ttcatcaatt tctcaggctc catcttacca actacaaata
     7741 aaaccatatt gcttggctga taaaacgtcc gatagcacgt atacaagtct tgagctgtga
     7801 ttttgtcgat actttcaacc gtgcccgcaa tatcgatatg caatgggtgt gtaggataca
     7861 agttatttag tatgccaaaa aacatccgcc agttagggtc gtcttcatac atttggattt
     7921 cctgaccgat gattcctttt tccttgttta cagtttcttc tgtgaagtaa ggtgcttgta
     7981 caaaatcgat cagagtcgtc agattctttt ctacctgatc cgtcgtagaa aacagatagc
     8041 tggttttcgt aaaacttgtg aaagcattgg cggaagctcc ttgtttccca aataattgaa
     8101 agacatcacc atcttctttt tcaaacaact tatgttctaa aaaatgggca attccatctg
     8161 gaactttcac cttttctttt tcaccatagg gaataaattc gttatcgatc gaaccataat
     8221 tcgtactgaa caaaccatat gtcttgtgat agtcattttt aggcaacaaa tacacggtca
     8281 aaccgtttgg cagtacttca tgataaagtg tttcattgat ttgctcatat tctgttttat
     8341 tcatttgcat ttcctcctgc taaaaagaag attgcctgca attcgatttg ctcagctact
     8401 tgttgtatct ctttgatcgt tacgctctcc atacgtttca accattcttc atcagataat
     8461 tttgtttccg gcagccagct gtccaaaaaa gaagcttcga ttgccgcttg cggagagtct
     8521 agagaaagca gatattggtt tctcagcatt gcttttgtct gagacagttc caactctgtt
     8581 atttcaccat ttcgcaaact ttctaattgc tcgtgaatca gatgaagtac ttgatttcgg
     8641 ttcttttcat cgataccggt ttggacactc atgaatcctc ggaacgtatc cacgctacta
     8701 gaggcataat aagctagact ttctttctct ctgacattca tgaaaagttt agagtgagga
     8761 aaaccgccaa ataaaccgtt aaagaccatt agcgcaaaac gttctggttc atcgtaatac
     8821 acatttgtct gataagctag gttcagcttt gcttgaacga ttggctcacg tacttgtcgt
     8881 tccttcacga tattcacttg aggttgggta taaaacagat ctggataaac tgtttcccgc
     8941 gactcaaatg gtaattgccc aactgcttca gctgctttgt ccggatcaac atcgccgaca
     9001 acaaaaatgt cgatttgatc atttgccatc atttcttgat agtaggacgc tagcgaacta
     9061 gcagtgatct tgtccaaatc ttcgactacg cccaaccctg ggatcttttg ttccgggctg
     9121 ttttcaaaat aaagttcttg caaggcatag gaagcaaaag tttgtttatc ttccttcaaa
     9181 ctttccaaat atagacgcag attgttcttt tccaagtcaa acgtcaactg atcaaactga
     9241 tgatttttaa tatttggata aaataaaacc tcttttaaaa aatctaccgc ttgagaaaaa
     9301 aggtttggat cattgacata ttcaccattc acaaaactga tccctgcgtt tagccaatgt
     9361 aaatttcctt tttttctaac tgataatcca aagcttgcgc cataaagctc tgcaagttgc
     9421 gcacttaact ttgtctgatc cgaataattc aaactatttg tttccaacat gctgctgaga
     9481 agtgaacgtt tcgtgatgac ttcttgttgc agacgcgcag taaaacggat aaaaatccgg
     9541 atcgttttgt attgttttgt tggtaagaca tgaagattca cgccttctgc caattgatac
     9601 accatattta tatgctcctt ttcacagttg attgcccgct tactctttta atgggtcaac
     9661 ctgccttttt tacttattcc tctgcttgaa atttataagt tcaggcaatt cgccgttttt
     9721 tctaaatgat gttacacttt atagtatagc atagatgttt tttatcaaaa gaaacagacg
     9781 gctaaaagcg tatattgttt ttttattttt tatttttcga tatactaaag acaaataaaa
     9841 gaagggaggg tcattcatga tcaaagaatt caaagagttc atcatgcgtg gagatgtctt
     9901 ggatcttgct gtcggcgttg ttattggtag tgcgtttaca agcatcgtaa accaagtggt
     9961 gcaaggcttg atcacaccac taataggttg gtttgtcgca atgatcacag gtacttctga
    10021 tttagaagga gcactttcaa ttcttgactg gtcgccgaca aaaggcgtca catttgcgtt
    10081 tggtaacgtt attagtgcta tcattacatt tctgatcact ggttttgtct tattcttagt
    10141 tgtcaaagcg gcaaaccgtg cgaagaattt acgctccaaa gaagagcaag tggaagtgga
    10201 agaaactcct actgcagaag attatcttag tgacatacgc gatcttcttg ccaaacaagt
    10261 ggcagaagac aatacacatc cagtagagca aacaaatctt tccgatactt ctaaataata
    10321 aagaatctaa aaaaaagaaa gccgattttc agtgtcggct ttcttttttt agattcttaa
    10381 cttgctactt gttccttttt catgtcgata tgtggaatat catcttccaa atacacatct
    10441 gagattgcct tgaatccaaa cgactcataa aaagcttgta ggtattcttg tgcgccgatc
    10501 acgattggtt tttctggaaa atttttctgg atcatgttaa tcgtttcttg aacaatcttt
    10561 cttcctaaac catctttacg gttgtcctga tgaacaagga cacgtccaaa atggatactc
    10621 tccgcttctt catatactcg cgtgtaggca aggatcttcc catcagtatc ttgaaaaaac
    10681 gtatgccaag cttctccatc aatgtcatca acttcttgat aaggacactg ttgttccaca
    10741 acaaaaactg cgattcgttc tttgacgatc gcgtaaaact ctgctgttga caattcttca
    10801 aatttcttta cctgatattc cactaagcaa acgccccctg cttttttatt cttagcatac
    10861 cgattcgcac acaaaaaagc aaacgaacct tacgaatctc aggctcgttt gctttttaaa
    10921 ctgcttcagt taaaaattcg atctcaactg tccgagtaag tacatctgaa tagctgtaag
    10981 atactcgttc aaaagaattt tcttcaggat ccaaatcaac aacgaatact gaaggatagg
    11041 tttctgttaa aacgccgcgg cgttctgttt gacgctttct tcctgtctga gcaaccaaca
    11101 tgattgggct gccgatacga ccttctaaat ctttcttgat tgaagctaaa gttgttggca
    11161 tacagttcac ctctactatc aactatacca catattttta aaattttcaa gtttaccata
    11221 aaaaaaaaag aaatgcaata aaaataaata agaagctcat taagattttt ctaaattatt
    11281 cctcattata aaatttcaag tgttttttta ttttgagttt tactctcccg taagcgctca
    11341 ttacttgctt gtgatccatt gctaaacatt cagccatgtc tacaaaatca acgccttgca
    11401 tataataatg aaaaatttta gcttctattt ttgaaaactg gatttcgcaa ttttcgattg
    11461 cttcttttaa taaaagatgc tgaattgcta aaggttcttg caatgtgatc tctgctaaaa
    11521 agtctggtcc ttctgtctct attttttggt cgattgagac ttcatctttt tgatcacgac
    11581 gcttcagcgc gttctgcttc ctcaacagag aatagatttg attgcttata acgcttttat
    11641 aataacttcc aaagctaaca cctttgcttt tttcgtaagt tctcaatgcc tgatataaag
    11701 cgatccttgc ttcttgttcc caatcctcag aaacataatt tcttaaataa taggtgtttt
    11761 tcaaacgtag taaaagtgga aaatattttt gatacagggc agcaaatgct tcttcgtcac
    11821 cattacgtgc agcgcgcaat aactcctcag acataaaata attcctccat aaattcgttt
    11881 atggaggaat cttatcatca atgattcatc gaactgatta gaacgatatg ttatttattt
    11941 tttctcatta aatcgtccaa tttttgggac aattttttta actgctcttc attccatgga
    12001 gaattacgtc ggaaatcaat aaaatgcata tctttatttt cttgggctat tgttttttcc
    12061 gtttttttta tcgtcttata taattcataa gcagaagttc gtaatgctcc ttgggaaaag
    12121 atcatccact gttctgctaa atcgcttgtc gccactgtca cttgtgtcaa acggtcattc
    12181 agctgaccag cgattctctc gatataactg tcagccgttt cgtcttcttt cgtaaaaaca
    12241 acatccaact ggtatttctt ataattttgt tggatgcccg gaactagttg ggcatcgaag
    12301 acaacgatga cttccattcc ttcatatttc gcataatttg ataaacgatg taaaagagct
    12361 tctcttgcat ccgccatttt gtcctgcttt ttaagctgga caagttccgg ccacgaacca
    12421 atcatgttgt agccgtctac aatcaacagc tgttttttca tggtctactc cccctgacgt
    12481 tgggaaaaag cctgatacat aagcaaacca gcagcaacac tggcatttaa gctctggacg
    12541 tgcccggtca tcgggatcgt cagcaattca tccatttctt ttttcaatcc tggactcatt
    12601 cctttgcctt cattaccaat gactaaagca attgatcctt tggcattcca tttttggaag
    12661 tttgtgccgg tcatatctgt gccaaatacc caaaatcctg tttctttgag ctgttgcaca
    12721 gcttgtacca aattcgtcac gcgtgcaaca gggacatgtt cgactgcccc agtggaagtc
    12781 tttgttacga taggtgtgat tccaactgct cggtgttttg gtatgatcac accatcgacg
    12841 ccacttgcgt ctgctgtccg taaaatcgac ccaaaattgt gtggatcttc gatgctatcc
    12901 aaaatcaaaa agaaggggtc ttcttttttc tcttttgttc gttcaatcag ttggtcaagg
    12961 gataaatatt cataaggtgt aatcgcaaga acgactcctt gatgcacgcc gtgatcgctc
    13021 aaagtttcta atttttgttt aggaacccat ttgactggca cagcttgttc acgtgcgatt
    13081 tctttcagtt cttctacttt ttctcctcgt gaatcttctt gaagaaagag tttattccct
    13141 cgcccttgtt gtagcgcttc tactgcggca tggtgcccaa agacgaaatt atcttctaat
    13201 gcttcttcat tttgatcaga aactgactga tttcgtccag tttgcttttt atttgaatat
    13261 ctattttgtt ttttgtcttc aaatccacgt ttcgattgat gtttgtttgt tctttcttta
    13321 cccatttttt tcaccaactt tctgaataca ccagcggatc aattcttcca tccgctcggt
    13381 ttgttttgtc aaatgcagat agcccatgag cgcttcgaac cctgttgcga cccgatacgt
    13441 cgtaacatca gcattttttg cgctcgtatg gcttttagca tttcttcctc tgcgataata
    13501 aagttcttct tcttctgtca aaagatgttc ttccagcatt tgctgaatca aaaaggcttg
    13561 tgcattcgca gaaacataat tcgttgccgc acgatgcagc ttattgggtt ttgtttctcc
    13621 ttgatcaatc aggtggtcac gaacatagat ttcgtaaatc gcatctccaa cataagccaa
    13681 ggctaaaccg ttcaattgtg tgtaatctct catgcttctc ttctccatcg ggttccttgc
    13741 ggtgtatctt ctaataagat ccctttttct ttcaacaaat cacgaatttc gtcgcttcgg
    13801 acaaaatcac gattgcttct tgcttgattt cgttcttcga tcaattgttc aatctcttca
    13861 tctaataact cttctgctgt aaataaaata ccgaagatcg aaagccattg cgagaataaa
    13921 gcttctcctt gtgccaagac agcggcagaa actttcggct ggtctagata agtgttcagc
    13981 cacttagcaa gctcatagac cactgtgatc ccatttgctg cattaaaatc atcgtccatt
    14041 tcttcgatga atcgctgttc taaattttct aacaaagcga ttttatccgc atcatctgcc
    14101 aaattctctg aggcagtttc ttggcggaat ttcaaattgt caaacgtatt cttcagtcgt
    14161 tgtaaattgg tggctgcttc tttcatcgtc gtttcactgt agcggatcgg acgacggtat
    14221 tgtgtggtcg ccatgaagaa acgcaagact tgtggatcaa cgtttttgat catttcatgt
    14281 acagtgatga aattccctag agatttactc attttttcat cgtcttcgcc aatcgttaca
    14341 tagccattgt gcatccagta attagcgaat tttttccctg ttttcgcttc actttgtgcg
    14401 atttcatttt catgatgagg aaattctaaa tcttgcccgc caccatggat atcgatcgtt
    14461 tctcctaaat gttttgttgc cataacggaa cattcaatat gccaaccggg acgtcctttg
    14521 ccccatggtg aatcccaaga aatctcgcca ggttttgctt gtttccataa agcgaaatct
    14581 aatgggtctt ctttttgctg ctgttcttcc cctgtacgct gacttgctcc gacttccagt
    14641 tcatcgatgg attggtcact taatttccca taattttcaa atttacgggt acggtaatac
    14701 acatcacctg aagattcata agcatagcct ttttcaacca acacttgaat aaatgctaaa
    14761 atatctggca tatgatccat gactcttgga tgcagtgtag caggttcgac attcaacgct
    14821 ttcgtatctt cttcaaatgc tcggatgaaa cgatcagcaa cttctggtgc tgtaatacct
    14881 aattcgtttg ctgtacggat gattttatcg tctacatccg tgaaattcga tacgaaatcg
    14941 actttatagc ctcgatattc aaaatagcgt cgaattgtat cgaaagcaat ggtgctacga
    15001 gcatttccga tatggatata attatacaca gtcggtccgc agacatacat accgacatgt
    15061 cccttttgta tagggatgaa ttcttctttt tttctggtca atgtattgta gatttttatc
    15121 attctgatct cctcactttt tctcctttga ttcgaacgac ttttgctggt actcctaccg
    15181 ctgtagcgtc gtctggaaca tctttcaaca caacggcacc tgcaccgatt ttggcgtttt
    15241 ttccgatcgt taccggtcct aatatctgcg cattcgcaga gagcatggct ccttgtttaa
    15301 cagttgggtg tcttttaccc gtttcttttc ctgtgccgcc taatgttacg ccatggaata
    15361 aaatcacatc gtcctcgatt tctgcggttt cgccaatgac gattcccatt ccatggtcga
    15421 tgaacacgcc agttccaatc gttgctcctg gatggatctc aacaccagtt aggaaacgcc
    15481 agaattgagc attgatcttc gccaataaaa agagccggtg ctgatataaa aaatgagaga
    15541 aacgatgcca aaacaaggca tgaaaaccag ggtatgtcaa aacgatctca gctgtcgaac
    15601 gtgcagccgg atcgtttctc tttgctgcat caatcgctcg ggtaaaccaa ctcatgactt
    15661 tccactcctt tcgtaagaat tttatgtagg aaatcatctt cttataaaga aaagaagcgc
    15721 cttttaaata agtttcctta tcaaaagacg ctttccgcgt ggttccactt ttcttcataa
    15781 tcaatgatta tctctgacag ctaacgcctg ttatacgttc ttggctactt tgtttcctat
    15841 aaaacagttt ttcctgtttt ctgtgtttcg ccaagaccac tcccagaggc atttcggcaa
    15901 ccattcattg ataccttgca ccacccggta tctctcttgt aatgatcagc tgcttacttt
    15961 tttctgttca tcgtgtttag ttcagtactt gatttaaatg tgctaatgat ttttcacgac
    16021 caagcaattc gatcgtttca ggcaattctg gaccatgcat ttgtcctgat acagctacac
    16081 ggattggcat gaaaagattt tttcctttta cgccagtttc tttttgaaca gctttgatgg
    16141 cagctttgac agttggagca tctactactt ccatttgttc tagctgtgct ttgaatgcgt
    16201 ggagaacagt aggaactgtt tcaccagcaa gtacttcttt tgctgcttca ttcaatacag
    16261 gatgttcatt gaaaaatagc gtagacagat caacgatttc tgcagcatag ctcatttgag
    16321 gttgatacag gctaacgatc ttcttcaccc attcgattgt cttttcatct gggttttctg
    16381 ctacacgtcc atcagcgatt agataaggca gacacatgtc agtaaatgca tctagatcca
    16441 ttgctttgat gtaatggttg tttacccatt gcagtttttt cgcatcgaaa gctgcgggtg
    16501 atttacttag gcgttttgga tcgaacattt tgatcaattc ttggtgttcg aagatttcgt
    16561 cttcgcccac aggtgaccag cccaataatg caataaagtt gaacattgca tctggtagat
    16621 agcctaattc gcgatattgt tcgataaatt gcaagattga ttcgtcacgt ttgcttagtt
    16681 ttttacctgt ttcactgttg atgatcaatg tcatatgtcc gaaggttggt ggtgtccagc
    16741 caaatgcttc atagatcatc agttgttttg gtgtattggc aatatggtca tctccacgta
    16801 aaacgtgagt gattttcatc aaatgatcat cgaccgcaac agcaaaatta taagttggca
    16861 taccatcgcg tttttggatg acaaagtcgc caccgatatt atcggattcg aacgaaatag
    16921 cgcctttcac catatctgta aatgcgtatt ctgtattgcg aggcacacgg aaacgtacaa
    16981 cagattctag accttgtgct tctttttctg cttgttcttc aggtgtcaag ttcgcacatg
    17041 ttcctgcgta atgaggcatt tctcctcttg cgcgttgtgc ttcccgttct gcttctaatt
    17101 cttcttctgt gcaatagcat ttgtatgctc gattgctagc aagcagctga tcaatcagcg
    17161 gttgatagat ttctttacgt tcagattgac ggtaagggcc gtattctcca ggattttcag
    17221 gagattcatc ccaattcatt cccaaccaag caaggttttc caactggctt ttttcgccat
    17281 cttcgatatt acgtttttgg tcagtatcct caatacgaat aataaagtct ccatcattgt
    17341 ggcgggcaaa caaataattg aataatgcag ttcttgcatt tccaatatgc aggtgcccag
    17401 ttggacttgg tgcatagcgt acgcgtactt tcgtcatttt cttcctcttc tttcaaacta
    17461 gactttgact aacagcaaaa attccgtcag ttacggcttc tcaaatatca tttatttaaa
    17521 gccactcagc acaaatttta ccttttctaa ggtaaatagt aaagccaact ggttgaattt
    17581 ccttaccagt tggctgaatt tcttttttat tttgattgtt tagtcttttt ctctgtctct
    17641 tttttattgt cgattcctcg tcctgcatgt gcaggtttag caaagatcat gcgtccagca
    17701 gctgtttgaa gggcacttgt tacaacgact tcgatacgtt cattcatgta atgctgtcca
    17761 tcttctacga caaccatcgt gccgtcatca aggtaggcta ctccttgttg gcgttctgtc
    17821 ccggctttta ctactagaac attcatcgtt tcaccaggga tgacgactgg ttttacggca
    17881 tttgccaacg cattgatatt aagaacaggt acattttgga attcagagac tttattcaag
    17941 ttatagtcat tggttacaat gacaccatct aaaagcttag ccaatttgat caatttgctg
    18001 tctacttcac tgatatcttc aaagtctcca tcgtacattt cgacagatat accctcttct
    18061 ttttgcaagg catttaggat atctaatccg cgacgaccac gcacacgttt caaactatcc
    18121 cctgagtccg cgatgtattg tagttcgtaa agaacaaaat tagggatcat caaggttcct
    18181 tctaagaatc cagtctttgt aatatcataa atccgaccgt caatgatcac gcttgtatcc
    18241 aagattttat atttatggaa attttctcct gattttcgtt ctaacagttg actctcaact
    18301 tgactagctg tttcttgctc gatttttttg cttcgtgaac caaaaacttt cttccattct
    18361 tcgattcgtg tcgttcccat tctcaatccc agatagccta agatgatcat cagtaaaatc
    18421 ggcaatacac tattgacgaa tggaatttcc atattgtaaa gaggggtcga gatcacaaca
    18481 cctaaagtca atccgatgat cgcaccgatc gcaccgaaga gaagataagt caagctgacc
    18541 tcacttagtt tttgttctac ttgtttgata atagctgaga tatgtttggc caacagcaaa
    18601 gataataaaa agaaaataag tgcgcctaac aggctgttgg taaatgtatt attcagccat
    18661 ccgtttgact gttgaccgat tgcctgccaa gctgagggta aaaatgaaat acccaagctt
    18721 gcgccgacaa tgatcatcag caaagtaatg acacgttttt gcatcgtttt tcctccgttt
    18781 cgacaatcat ttattagtta gcggaacaca cgcttcaacg tttcagccaa agttgctact
    18841 ccaactatct cgattccttt tggcgcttcc cagttaccta agttgttttt aggtaggtat
    18901 acttttgtga agccgagttt ttggacttct ttgactcgtt gttcaatatg actgacacgt
    18961 ctgatttctc cagtcagtcc aatttcaccg atgaaacatt ccgttggctg tgttcctttt
    19021 tctttatagc ttgaagcaat actgacagcg atagctaaat caatcgctgg ttcatttaat
    19081 tttaccccac ctgcagcttt taagtaagca tcttgattct gaagaagcaa cccagctctt
    19141 ttttccagca ctgccatgat cagagatacc cgattgaaat caagacctgt tgttgttcgt
    19201 ttggcatttc cgaacatagt aggtgtgact aatgcttgga tctctaccaa aattggacgt
    19261 gtcccttcca ttgccacaac gatagcagag ccagttgcac cgtctaggcg ttcttctaag
    19321 aaaatctgtg aaggattagc gacttcttcc aatccatgct catgcatttc aaaaatccca
    19381 atctcattcg ttgagccaaa tcggtttttt actgctcgca aaatccgaaa actatgatgt
    19441 tgttcacctt caaaatataa caccgtatcc accatatgtt ctaacattct tgggccggcg
    19501 atcgagcctt ctttggtaac atgaccgacg ataaaaatgg ctatcccatt tgttttagcg
    19561 gttttgagta gttctgcagt ggtttctctt acttggctga cacttcctgc cacacttgtg
    19621 atgtctggct gcgtcatcgt ttggatggaa tcaatgataa cataatcagg ggagatatgt
    19681 tcgatggcac gactgatttc gttcatatct gtttcagcgt atagataaaa ttctgtgttg
    19741 atacttccga ggcgttccgc gcgtaatttt atctgctctg cgctttcttc gccggataca
    19801 tataagactt ttccgcctat ggctgctaat tgctgagaaa cttgtaacag caaagtggat
    19861 ttcccgatac ctggatcgcc accaatcaag accatagacc ctggcaccac acctccgcct
    19921 aacacacggt tcagttcttc taattttgtt ttgatccgag gttctttttt agggacgacg
    19981 tctgcgattt ttgttggttt tgctttttct cctgtcaaac ttgtccgagt tctgcggtcc
    20041 gttgtatctt gttcgatttc ttcgaccatc gtattccatt ttccgcaatt tgggcagcgt
    20101 cccaagaact taggcgacac atacccgcaa ttttggcaaa caaattgtac ttttgatttt
    20161 tttgccattg taccgcctcc ttttatccta atcatgagac attaaatcaa gcttaagtat
    20221 ctttaacgtt ttatttactg gaagaaccaa atccgccttg acgttgagca gtcgtttgat
    20281 cttgatctgc ttttaaaaac ggcaaaaaga tcccttggcc gatacgctcg ccttttttga
    20341 tcactacatc cttgaaacca aagttggtga attggaacat gatatggcct tcatttgcct
    20401 cattattgta ataatcactg tcaatcacac ctactccgtt tgtcaacact aaaaaccgct
    20461 tcaacggatt gcttgaacgg ttagcaagct gcaaaaattc atcttctccc atgtaagctt
    20521 tgattccagt aggcacgagg gttggtttca attgtttttc attttctgat acatcttttt
    20581 tcaatccatc tgcgactgtt tccaacccct ttttccagcc tgctttccag atacttggaa
    20641 tcacgacctc ttctgctgct tcaaaatcat agccagcagc atgatgtgta gcgcgctgcg
    20701 gtagattgat attttgatct ttatatttag aaataacttc aaatcctctt tttctcaccg
    20761 gttttcctcc ctaaataaac tactcctatt ctaacatatc cgaggctgta aaagactttc
    20821 tttacaaatt cttcaagaaa ttctgctatc ttaaagtaaa ccattaattc gttctttttc
    20881 caaacgactg caagttgtac aaaaaaacta ttttttacat gactttactc gtcaaaacaa
    20941 gtagaataga acgaaattaa tgagtggata tttgattgag gtgtcacatg cgtaaagaaa
    21001 ttaaaaatgc aaaacgaatt attgtcaaag taggaacaag tacattgatt tatcccaatg
    21061 gaaatatcaa tcttgcggca atcgatcagt tagcttttgt cctttctgat cttagaaatc
    21121 ggggaaagga agtgatctta gtctcatcag gagctgtagg cgttggtatg cataaattac
    21181 atttgtcaga acgtccgact ttgattcctg aacagcaagc tgcggcggct gttggtcaag
    21241 ccgaactgat gaatatctat aatcaaagat tcagtgtata taatcaacag acggcacaga
    21301 tcctcgtgac aagagacgtg gtagattatc ctgaaagtcg taaaaatgtg aggaatacat
    21361 tggaacagct tttgattatg gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag
    21421 tggatgaatt agatcatctg acaaaatttg gcgataatga tcagctgtca gccatcatgg
    21481 caaaacttgt cgcggcagac cttttgatcg tcctttctga tatcgacggg ttttattcag
    21541 ataatccgac tacaaatccc gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa
    21601 agattctggc acaagcaggt ggtgctggta gcacatttgg aacaggcggt atgcagagta
    21661 aattaaaagc cgcttctagg atatttgaag caaacagcgc aatgatttta gcaaatggaa
    21721 aaaatccggc aattattttt gatattcttg caggaaaaga agtaggtact ttatttaagg
    21781 aggacttgaa atgactgact tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca
    21841 actagcactt atgcctacaa accgcaaaaa tgagctactt gttcaaatgg cacaaaccat
    21901 caaaaagaat caacaagcga tcattgaagc aaatcataaa gacttagaaa aagcagtcga
    21961 aaacaatatc agtgaaacga tgcaagaccg cttgcagctg acacctgagc gaatcaatgc
    22021 catggctaca gagatcgaaa aaattgcttc gttagacgat cctattggaa aagtagatga
    22081 gatgtggacg aacaccgacg gattgagaat cggcaaaaag cgcgtgccac taggcgtgat
    22141 cggcatcatt tacgaatctc gtccaaatgt cacgacagat gcagctagtc tagcattcaa
    22201 atcagggaat gccgttatcc ttcgcggtgg caaagaagca ttcttctcga atcaattatt
    22261 agtccaatta ttgcagcaag tactggtcag tgctggcgaa tctccttatg ccatccagtt
    22321 tgtcgatgat acttctcatg aaactgcagg acagttgatg cgattgacag aatacttaga
    22381 tgtgctgatt cctcgaggcg gagcaaaatt aatccaacga gtcaaagaac aggcaacggt
    22441 acctatcatt gaaacaggta caggaaataa tcacgtatat atcgataaag aagcacagtt
    22501 atctatggca gtgaacatca tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc
    22561 ggcagaaaca ttgctgatcc attctgagat cgcacctttt ttcttgccag ctattgaaaa
    22621 agaactggta gaacacgggg tctctttacg tgcggatgct cgtgcgttag aatatctaga
    22681 gacggctgct ttagcagaag acacagattg ggatactgaa tatctagatt atatccttgc
    22741 tgtcaaagtg gtcgattctt tggatgaagc aattgcacat atcaatcggc ataatactaa
    22801 acattcagaa acgatcgtga cagatagtta tgcggctagt caacgtttct taaatgaagt
    22861 ggacgcggcg gctgtctatg tcaatgcctc cactcgtttt acagacggat cggtatttgg
    22921 ttttggtgca gagattggta tctctactca aaagttgcat gcccgtggtc caatggggtt
    22981 aaatgaatta acttccacaa aatatatcat ttatggcgat ggacaaatcc gcgaataatg
    23041 tttcgtgtga aacattattc ccgccttttg ccttttttct gtaaaggttc tatccaacta
    23101 gaaaggtgtc aatatgtttg aaattttaat gatttttttt atttacctta tttctctgaa
    23161 tatcgctgct tttctgggag tcggtatttt atcccttttc tttcaattca aaaaacgaag
    23221 catagggagt caacgggaaa aatggtcgca gtattttgac aaaatcgggc aaaaagggct
    23281 ggttacacgc ttgcacatta gttacatggt tgcattatgc ttattagcga caatcaatta
    23341 ctattctttt ttcgatcatt caatcgctta tacgattacg ctactgattg ctgggatttt
    23401 tcacttatct tataaatatc agctgaataa aaaccacttg aatcggactt tccgctaaaa
    23461 aatatccgta cgataagtag agtgccttga agatcggcaa aaaactgccg gactttttag
    23521 aggctgtgac aaagattttg tcacagcctc tttttccata taaacggcgt tcgcctacca
    23581 gctgcctggt aaatctgttt cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc
    23641 atcataataa cgaagcagct catccatgga caagttcatc gttttttctt tttcttcttc
    23701 cgctgattca gccagtagct gttcccgata cacctgatac ataccagcta ttttttgctg
    23761 catcgctttt ctagtttcac cttccgcatt gattattggc aattctttga tatagccacg
    23821 ataaatcaac cgatcatctg aaaattcaat gggtaattca acgagtgtca acccgacttc
    23881 ctgaaaatat tcttccagca tctattatcc ccccattctt tttcttaaat gaattttagc
    23941 ttacctactg aaaattttcc agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa
    24001 tcgtgtcaca tccccttttt ccgaataaat ggtgttcttc tgccaactcc tcaatcataa
    24061 gtcacaatat tcaacaaaca tgagaaactg ttttttgaaa ttgttccttg tgattcagag
    24121 ttaaacgaca gtctcacaac ctcttggtaa acggcgttca atcagctgac cctcggtatt
    24181 agctcaaaaa acggtaaaat atgagaagct atttttccgt tttgtttctc taatacttag
    24241 gataaaacgc tgattttaca acctctgtta aacggtgttc aatcagctgt ccttcggtat
    24301 tagctcaaaa aacggcaaaa tatgaggaac tatttttccg tttgtttctc taatactcag
    24361 gacaaaacgc tgattttaca acctcctgat aaacggtgtt caaaagcttg catctgcggt
    24421 aataagccca accaaacgaa aaatatgaga agctattttt gtttggctgt tcttatgact
    24481 tgatgcagga cagctttttc acaacctctg atttcttatt cttcttcatt tgtagcaaaa
    24541 taattacggt gataaatgat acaacataca ctgataagaa caatggcacc tgcaaaataa
    24601 aacgcatagc tttcaccaat atgttcacca atcacacctg caataaatgg tgctaaagct
    24661 ccaccgaaaa agcggacaaa actatagctg gacgaagcaa cgtttcgttg cagacctggt
    24721 atttccatag caatcgtcgt caacagtgta ttgataattc cttggaaaaa tcctgctaac
    24781 acaatgctga tcggtacaag gactggcata gaagttccag ctcctaataa cagcaaacaa
    24841 ataatgaata agacaaatgc tgctaaaatt gtccgataag tcgtaaactt ccgctcaaga
    24901 gccggtgcaa cgaaaatcga agcaatagcc agaagcacac cccagccaaa aaatacaaaa
    24961 ccgacttcta attcagaata gccaatcaat aaaaatggcg catacgcaag taaagtaaag
    25021 aatccgaaat tatataacaa tgcgattagt ccaactgaac gaagtttatg attgcttaac
    25081 gctttgacac cttcccaaag gcctactttt ctctttggtt tctcagtttc aggtaaaagt
    25141 accgctaggg ctataaaacc gcaaaacatc aaaaatgcta ccccaaaaaa tggataacgc
    25201 catgaaccac tgcccaagac cccaccgact agagggccaa cagccattcc gcagccaagt
    25261 gctgcttcat acatcatgat tgctttttct gtgttaccaa ctaaaatact gacgatcgtt
    25321 gctaaagcag tagagacaaa taaagcattc ccgacacccc agccggcacg taacccaatc
    25381 agcattccga tattcccaga taatccgcca agaccagcga atacaacgat gataaacaat
    25441 cctgtcatca atgttttttt caaaccgatc cgactagata agaaacctgt aaatagcatc
    25501 accacaccag tgactaacat gtaactagta aataacaggg tcgtttcagc tggcgtagcg
    25561 tttaactgtg cagcaatcgt ttttaagatt gggtctacta gacccactcc cataaaagcg
    25621 atcatacagg tagctgctac aactagcgct gaacttttcc cctttgtttc ttcatttgtt
    25681 tgttcttgta tatctatttg attctccaat atttccatcc ctttctctct attagcatca
    25741 tacatgattt attttacatg tcaatatgca catgtaaaac gtggaaactt tactgtgata
    25801 taataaagaa aataagacga gaggtaggat ttttatgaat actttagcct atatattatt
    25861 atgcgtgctt ttatccaaac cacgttctgg gtatgaattg aagcagctga tcacaatttt
    25921 ttgggaagcc catcacagtc agatctatac aacgcttgcc aagttagacc agcagggtta
    25981 tgtgaccgtt ttggacaaag acgagcactc tcaaaaaaag atctaccact tgactgatga
    26041 aggacgtgct cttgtagagg aatggatcaa agaggaaact cccgctccta cgcaaaaaga
    26101 tgaatttcta gcaaaaatct atgctttttc gacgctcgat aaaacaacag ctactggact
    26161 gctattcact agagaacaac agctgaacaa aatcatgcaa aaaaatcaaa aaaaattgaa
    26221 tgacctctcg ctagacaaga aacaagaatt tggacgttat gttgtgatcc agcgcagaat
    26281 tatgctttgt cagcaggaac tccaatggtg tcagcaagta agagaacaaa tgcagcagtt
    26341 ttttgagtaa gggataaaca gaacctttct agaaatatct attgaagagc aaattatctt
    26401 caataaaaga gcaatacagt tggaaaagaa aaagcgatcc gaacgacagg ccaaaaccta
    26461 tcgttcggat cactattttt ctagaactgc attcaagaga ctatgacaaa aagcatgcca
    26521 aggccttttt tcaaataaca gtgttccatc attcattact ttgaattgct tttagcacac
    26581 ttcttgaaag cgggccaacg atgatcaact gcaacggtaa agcggcaatc acattcattt
    26641 tccaagcatt catataagca gagaataatt gatcaggtat tccttgttcc atgatcacac
    26701 cgaataaaga catacatgtc accattccta aaaccatcaa gcatgaaata gttaagatca
    26761 tttggacctt gctttctgga tttactggta gtttaaaggc aatttttttc gccagcacac
    26821 cgatgatgaa gacatccaaa atgaaagcca ccacgaatcc aggcactaga ccaatcaaca
    26881 aacttgataa atgaagatct tgatgcagcc ataaattata cgcgctcatc cccaagacca
    26941 tcaaaaaaca catcattgtt gtaaaaatta ttccttcttt tttatttgta ggcaatctgt
    27001 accactcctt taaatttgtc acgaaaatca tcgtagcaca tttaaaaatt tctcgtaagt
    27061 acttttttta ttttctgccc ttttatttca taaacttcat cagccgtacg ctcgacaaag
    27121 taagaatcat gagaagtaaa caaaatcgtt cccggatata tttgcattag cttctctaaa
    27181 gcttcgatcg ttgccatatc aataaaatta gtcggttcat ctaacagcaa cacattactt
    27241 ggcttagtaa aaagcaaagc aatcgtcaga cgggtcgctt ctcccccact taatgtacat
    27301 aacggtttcg tcaaagtttc ctgagcaaac cctaagtgat tcagcaaact acggacaagc
    27361 gattctgaaa actctgtttg tctcatgaca aaacgaatga tggattcttc agatgtcatt
    27421 tgataaccct tttgctgata tacttgaaag cttactttcg gagagagaag gattccttct
    27481 ccttgtttgc ggatatgttc taataaggtt gtcttacctg aaccattttc accgacaatc
    27541 gcgatttttt tacctaaaga aaactgaaaa tcaccattta ccagtaatgt tctcccactt
    27601 ctttccaatt ggacattttg tcccatgatg ggatagcggc tgtggagttc aagagatttt
    27661 ggtactggaa aacggatttg tttcatctgc tgcggcttgg ttgtttctcc tattttttgg
    27721 agtcttctct ctaatgtttt cgcattcttt tggatggctt tttgtacact gtctttttgt
    27781 ttagaggaag acaaccgatc cagtctgatt tgttgctttt tctttttact ggacactttt
    27841 tccaactttt ccgcttgttt tttcttttct tggatcgatt tttccaatcg ttttttctct
    27901 ttcatgatcg actctgcttc tcgtagctga gtctttttct ccaattcctt ttgagtgaga
    27961 taggcactat aattccccga aaactctcgg atttctccgt cttttacttc ccagattttc
    28021 gatgctagct catctagaaa atagcgatca tgactaacaa aaaccagcgt accataataa
    28081 taacgaagct cttcaatcag tcgatcaata ctttttctat ctagatgggt tgttggctcg
    28141 tctagaagca tcccttgttc ataagaagaa agaagacgta tcaactcgat ctttcgttct
    28201 tcccctccgc tccatttccg ctctgaagtt tcttccagtc ttaactggaa atgactggct
    28261 aattccaaat ttaggtcctt tgcttcagcg agataggtta attgaggcag gtagttgaag
    28321 gtgatctctg tttggatttt cccatgatcg ggaaaaagtt caccggctat tagtttgagc
    28381 aaagtcgatt taccgacacc attttctcct atgatcccta tacgatcttg ctgatagaca
    28441 cgaagagaat caatagatag ttctagctgg ttgccaaagc tgacttgtag atttgttata
    28501 tttactgcta aattttccat atgttttaca ctcctatttc atgtaaaaca acaattatcg
    28561 atactaaaaa agagcagaaa aatctgtttg caaaaaaact gcatcacatc ttttcctact
    28621 ctgaacgaaa taactatgac taaatcacca aatctgtcat agctttcacc tattctactg
    28681 tcgttcgaaa aaatggacat actccatcca tgaaattgtt gtttatacat gtcttttgaa
    28741 tttatttagt tgttattcaa aagttcgaaa cgtaatttca tcgatgcagt catttttccg
    28801 acctcctttt ttattaggtt accacacacg attcaaaaaa agcaatagct actgaatagt
    28861 aactgaaaac cttttattcg aacatagtga tcttttctgg tgctaaacga acagactcgt
    28921 aaccttcttc taccgctttt cctattgata aaataataac tggaacataa cggtctttat
    28981 ccaaaccaaa tgcctctgcc aactgatctg cttcgaatcc accaattgga tttgtttcat
    29041 agccatgatc acgcgcaact aacatgaatt gcatagccgc taagctagaa tcgactttga
    29101 ctacatcgtt catttcttct cttgaaaaac tcttgtaata aggaatgatc gcagctaatt
    29161 gttgatctct gacttctttt ggcatcttcc ctgattcgta agcttggttg tagatttctt
    29221 cgccatattc gtaacattgc atatcgccaa aaatcatgac cattgcagaa gaagtatcat
    29281 tttgtctcgt attgaaacgg atcaaaggac gcaagatttc ttttgcttcc tcgctttcag
    29341 cgaccacaaa gcgccatggt ttcatattga cagaagaagg tgcagtcgtc gcttctttga
    29401 tcatttccag catttcttcg tgtgggattt tgtatgtttc atcatatacg cgaacggatc
    29461 ttctaccagt cataattgtt gaaaaatcat ttttttgttc cattattatt aacctctttc
    29521 gtttctgttg ttatattgca aagttgggta ttcaatttat ctaaaagtgt tagaaattgt
    29581 ttcatttcta catcgctgaa agccggggat agcacggaac ctgtatcttt atggtccatt
    29641 tcacaatgct tgattttctt ctgggctttt tctgttagcg ctacatacac ttcccggttg
    29701 ttctgaggat ttctctgacg cgaaacatag ccatttgctt ccaatacctt cagatgacga
    29761 gtaactgctc cttgatcaat ctgcaacttt tcttgcaaac tgctttgtag caatggattt
    29821 tcttctttca gtctcatcat gatctcgtat ctagtaagac ttataccgat attttcttca
    29881 aagatctgga cgattttttg atcggctaat ttgatttgat ataaaaggtg actaatctct
    29941 tctaaagaca taatttcctc ctttctaata attgactcgt caacgattga cttgtcaata
    30001 atagaataaa aaaagaaaaa agtcaaagaa tcagctgtgt aataaaaaaa acgctctcaa
    30061 tagaaaagta tagagaaaag aaattttttg tagaatgaac ggaaaaaatg ataaagaaag
    30121 cacaacgcta aaaccaagat tattgattct tatcaaacta tagaagatag ctcttctgca
    30181 gtagataatt ttacaccgcc aaaaatttcc gaaatcgcct tttgcaagaa tcccgtcgaa
    30241 tattagaaat tggatcagta tgcttaaaca gtaataaaaa agttatgaca agatgttgtc
    30301 acaacctttt ttgagtaaac ggtgttcaaa aacttgcatc tgcggtaata agttcaacca
    30361 aacaaaaaac atgaaaatct gtttttgttt ggctgttctt actacttgat gcagggcagc
    30421 tttttcacta cctcttttta ataaccataa aatcctattc cttcatatct ccagcccatc
    30481 ggtgttagcc aatctctttc cttagcactc aaggtatagt gatgggtacc agttttttca
    30541 tttggattaa atagtcgata aattggcttg gaaccaccag aataaaaact tagaccttca
    30601 taacgccaac cgatttttgt taaagagttg atctcagatt gtgccattgt gtaatgatgg
    30661 tctccactgt aaggattgta taaacggtag acaggttgtc cgctgtttgg tgcttgccaa
    30721 gcaatccctt catattccca gcctacagtt ggcagataat ttttttctcc ggcattcaat
    30781 gtatataaat gttctcctgt atgacggtta tacagacgat acatcggaga agttgaagtt
    30841 ggtgctgtat catactgtgt cagattatag gtttcgatta cattgttcaa tgtagaacca
    30901 taattaggcg cagtggcata acgacctgtt agccaagccg tagcatcttt gtaagagttc
    30961 gtattgcttt tccatgcacc ggaatagtag taaacgcctg gctgaaaaga agtcgtcttc
    31021 aataccgtta cgttatcttg caatgattct ttgtacgaag gatattttct aaaagcggca
    31081 ttcaccgtaa tccattgccc attcacatat tccgaagtag gcatcgtaac agattggcca
    31141 ttatagcttc ctttaatccc aaatagattg taattagggg cagaagccaa agtactgttc
    31201 ccccagccac tttcaacgat ggcttgagca atcatgactg atgcatataa gtcattggca
    31261 cttgccaaac tttgtgcagt tgaggcaata ctatcaatga acgcttgttg agaattagca
    31321 gatgagcgag cataaaaagc atagccagta tagttttcga attcagacaa atctgcttga
    31381 tgggtatgtg tcaatgtttg cccatctgtc aattggttcg tgatttcttc tgcattctct
    31441 ggattgttct gagcttctgc tgatgtactg tcttcggttt gtgtttgttc tgttgtttca
    31501 gttgttggtt cttcggttga aattgtattt tctgagacag tagctgactg caaagtactt
    31561 tctgtatcgt tttcaattcc tgtttctgaa gaactcgatg tctgagttgc gacagtttct
    31621 gtcgattctt cagctagcac tgttgtgttg gcaactgcta gatttcctaa aagaataacg
    31681 ctaaataata aattctttgc tttcatcttt tttctccttt gaattatgaa acttttccta
    31741 aataacagtg taactaaaaa agtaaaagca agtcaatgtc catatttaaa taacatttgt
    31801 ttctttttaa taaatttgct ttttctgcat aaaaagacgt aaatgaagaa tattcttctc
    31861 cattcacgcc ttaaattgat ggtcgtttga ataactattt aacaagtaat tctctctgga
    31921 tattatccgt tatggattat acttcctgtg tcgtcggacg gataatcatg ttgctgacag
    31981 acatccggtc tggcgtttct atcgcaaatg ctacagcaga agctatatct tcagatgtta
    32041 atccccattc tttttgcgct tcatgaagtt caagagcagc ctttttgtcg ctgatggttt
    32101 gatataattc agtttgaaca gctcctggag agatgatcgt agatttgatg ttattttgcc
    32161 gttgctcttg acgtaatcct tccatgattg cacggacagc aaattttgtt ccgcaataaa
    32221 cggcggaatc aggataaaca acatgtcccg ccacagaatc agttgaaatg atatgcccag
    32281 atttttgttt ttccatgatt ggaagtacgg cggaaattcc atttaacaca cccatgatgt
    32341 tgatatcaag catattttgc cattcatcac ggtgaccttc aactaatgga gcggttggca
    32401 tgattcctgc attattgtac aatacatcga ttcgaccgaa cttttccatc gctaaatcga
    32461 taactgcttg gacttgtgca aaatctctta catctgctgt ttgaatatat agctcagctt
    32521 ctggtaaaga ttcttttatt gcttttaggc gatcttctcg gcgtgcagct ataactagtt
    32581 tagccccttt ttctgctaat agcttcgttg ttgcttcacc gattccgctt gaagcgccca
    32641 tgatgacaat cactttttct gataaatcat tcattctatt ctcctcctgt tttgaaaaat
    32701 ctcttgctta tactcactat ctcttatttc atcttactgt tcaaataatc cgttcgacag
    32761 gtttgacgat tttggcaggg attcctgcaa cgatggtatt gtctgggaca tctttcgtaa
    32821 cagttgcatt cgctgcaaca atggcattct cgccaatcgt tacgcctggc agaatagttg
    32881 ctcctgctcc gatccaagca tttttcttga tgtggattgg ctttactgac aagcctcttc
    32941 gttcttttgg atcggttaga tggttcaccg tgatgaggtt gacacgagga ccaatcaata
    33001 ctgaatcttc taaacagatg ccacctaagt caacgaacat cacttgacga ttgataaaga
    33061 catttttccc tatactaata tgtttgccga aatctgtata aaaaggcagc agaacagtca
    33121 ccgtatcatc tactttgtca ctaatgatat tttctagccg tttgcggata tctgctggtt
    33181 catggtaccc agtattcagc gctgctaaag gctcttcgtt ttccgcttgg atctgatgga
    33241 tcacttcaaa gagcggatcc gttactcgaa tctctttatt gacgatttgt ttttcgatga
    33301 ggcttgtcat tttgctccct ccttttatcc ttactttata acggacggct tatgatgtct
    33361 aatacttata aaacatactt aactattcct tttaactata cttagtggta tgctttttta
    33421 aaaacaggag ggaaaaaatg gatattcgta tactgacgta ttttttagca gtagctagag
    33481 aaaaaaatat cagccgagca gcaaaatctc tgcatatcac gcaacctact ctctcaaaac
    33541 aactgaaaga tttagaagaa gaacttggtg tgacactttt cactcgaggc tcccgagaga
    33601 tacagttgac ggattctggt cgatatcttt attcaaaagg aaaagaaatc ttgtcgttgg
    33661 tagaaaaaac ggcgaacaat ttaacaaatg acgcaatcat ctctggagaa atctacattg
    33721 gcggaggcga aactaaagca atctctttca tttctgatag ccttcattcg ttgatcaagg
    33781 cgcatcctga tatccagttt catttatata gcgggaatgc agatgatatc atagacaaac
    33841 tggatagcgg attacttgat tttggactcg taatcgaacc gacaaacaaa caaaaatacc
    33901 agtcatttcg actgccagcc caagatcgtt ggggactttt gatacatcgg aatcatcctt
    33961 tagcaaagca aacagtcatc catccgcagg aactgaaaac tgttcctttg atcatttccc
    34021 ggcaaacgtc agtggacagt caactagctg aatggctagg tttcagcata gatcagttat
    34081 cgatcgtcgg aacatataac ttattgtaca atgcttcttt gatggtcgat aacgggactt
    34141 ttggtgcatt agctctagat gggatcatca atacttatgg gacggaaaaa gtattcatcc
    34201 cactcgatcc gccattacaa gcttcactga atctcatttg gaaaaaagac cagcctcttt
    34261 ctcctgctgc tcgtaaattt ttagatgtcc taacggagaa atacagttaa gtagagtttt
    34321 tttccttcgc tttcagcttt ttaacaacga ataagttaag caaaagatcc aatgattaaa
    34381 taacataagt ttcttaattt gttccttcct tttatttctg ataattccag taaattacta
    34441 taattataaa aaaagaattg acaaaacata actgtctata taaaattgat tacgttaaca
    34501 ataattcgtt tcatgtgact agatcatact aggcatgaaa aatctctgag gaacatggtt
    34561 tccctaggag gtttttcgtg tctatttttg tttggccgca atcaactggt ctagcaaacg
    34621 tgaaaaccaa atcttcaaaa agggtgcgcg catgaaaatc aaaaagatct ttaatcaaaa
    34681 tgcagtattg gtagatgacg gtggacagga aaaagtagct attggaaaag ggattggatt
    34741 tgacagaaaa agaaatgatt tgatttttga tcgtgatgtc gaacggatat ttgtcatgga
    34801 accagaaggt caaatcaagt tgcaaaattt acttaatcag attgatgaac gctttttatt
    34861 tgcagcagaa acgatcattg atcatgcgga aactgttttg atggaaaagt tgaatgaaca
    34921 tgtattgatc gctttgaccg accacattgc tttttctgcg gagaacatta ataacggtat
    34981 cgtcatccgc aacaaacttt taagagaaat cgaagtgctt tacagtgatg aattcagtat
    35041 tgctcaatgg gcagtagagt atctgacaaa agagcttggg attccatata cttatgacga
    35101 agcaggatat atcgcaatcc atattcacag cggacgaagc ggtcggaaca ataaccatcg
    35161 cagtatccgt gaagtgacga ttgtatctga catcatccat ttagtggaat cagaattgga
    35221 aacagatatc cattcagaac cattttcact gaattattca agactggtga atcatttacg
    35281 actgcttcta caacgaaccc atgcgcaaca atatgctgtt ttagacacgg aaatcgtaga
    35341 aatggtcaaa cgaaaatacc cagaaagcta taagatttct aaaaaaatcc gtgtgttgct
    35401 gaccaaagaa taccagctcg cgatcacgaa agaagaactg ggttacttgg ccatccatat
    35461 cgagcgttta agaagcgcaa tcgtacaaac aaatgttaac aatcatgagg aggaaaaaaa
    35521 ttgaaagcat atctacaacg aatgggacgt tcccttcaac ttccagttgc ggtcttacct
    35581 gctgctgctt tactagtagg gatcggaaac tggtgggcat cttatagtaa cgacattgtc
    35641 gctcattttt tacaagctgg cggtaacgca gttttaggtc aattaccttt gctgtttgcc
    35701 gttgggttag cactagggat gtcagtggac aaatcaggtg ctgctgcctt ggcaggtttg
    35761 gtcgcatttg aagttccagt aaatgtttta aaaactgatt cagtagctac tttattgaat
    35821 atcaaaccag aatcagttga tccatctttt gctcagatca gtaacgtatt tattggtatt
    35881 attgccggat tgattgcggc agctctttat aatcgtttcc acgaagcaaa attaccgatg
    35941 gctttatctt tctttagcgg caagcgtcta gtgccaatct tggctgcctt tgtcatgttg
    36001 ctgatcagtg cagtgctact gctcgtatgg cctccggttt actctgcttt agtttctttt
    36061 gctaaattca ttttgagttt aggctggggt ggtgcaggtc tttatggctt cttcaaccgt
    36121 ttactgatcc ctacaggatt gcatcaagca atgaactccg tgttctggtt tgacgtagca
    36181 ggaatcaatg atattggtaa atttttggct tctgaaggaa caaaaggcgt aacaggaatg
    36241 tatcaagctg gatttttccc aatcatgatg ttcgggcttc cagcaggagc atatgctatt
    36301 tacagaaatg cacgtccaga aaacaaagca aaaacagctt ccttgatgtt agcagcagct
    36361 tttgcttcat tctttactgg tgtaacagaa ccacttgaat tctcatttat gttcgttgct
    36421 tggccattat atgtcttgca tgcaattttc actgggattt ctttagcagt gagtgcattc
    36481 ttccattgga cagcaggatt tgcctttagt gccggatttg tcgattactt cctatctttg
    36541 aaaaatccga ttgcgaatca gccattgatg ttgatcgtcc aaggattagt aacagcagtg
    36601 atttactact ttggctttaa ctttgctatc aaaaaattcc atctgatgac tcctggccgc
    36661 gaggaagcag atctttcaga tgacgatact gcaacgacta attcagacaa taaatatgcg
    36721 gcacaagcaa gcaaaatcta cgctgcatta ggcggagccg acaatgtaac ttcgatcgat
    36781 aactgtacta cacgccttcg tttgcaagtc aaagatacag gcctgatcga tcaaaataaa
    36841 atcaaagcca ctggcgtacc tggtatgaag atcatcgacg gaaaaaatgc gcaagtcatc
    36901 gtaggtacag aagtacaatt cgttgcagat gagatggcaa aactacatgg tggtgctgca
    36961 acacgacctg cacaaacaaa caccgtggtt aaaacagaaa catttgccgg tggaacgatc
    37021 gaacaagata tttacgctat cgcaaatggg aaactgattc caatcactga agtttcagat
    37081 gatgtattcg cggaaaaaat gatgggtgac ggctatgctg tattaccaga aaatggagaa
    37141 atcttctctc caatcgctgg tacgatcaca aacattttcc caacgaaaca tgctgtcgga
    37201 atccaaacag atgctggtat cgaagtcttg cttcatatgg gcatcaacac tgttgactta
    37261 aaaggcgaac cattcacttt atatgtaaaa gaaggtcaaa aagtcgcaag aggtcaattg
    37321 attgctttag ttgatcttgc cgctattcaa tctgcaggca aaaacacgga tatggtcgtt
    37381 gtattcacaa atggcgacaa agtccaaagc ttagaaatcg aacccgcacg tgatgtaaaa
    37441 gcaaatgata aaatcggctc tgtttccaac aaagcctgag ccatcgtctt ccccagactt
    37501 ttttatcgat aaacaaacag gagctgagac aaaaatcttg tctcagctcc tttttccgaa
    37561 taaaaacggc gttctcctgc caactccgcg gtaataagtc cagccaaacg taacatatga
    37621 agagctattt tttatttgac tcttcttatt acttgatgca gaacagcttt tttacaacct
    37681 ctgataaacg gtgttcaaaa accagtttat cggtatgaag tcaaatttat gcaaaaatgt
    37741 ggaaagccat tttcacaaaa tttttcttca tactcaaaac taatcggctt tttcacaacc
    37801 tctttataca ttattcctga tccaatattt cttttaatgc tgcttcaaga gaagttaatg
    37861 gttttcccaa tactttttct aaatcatcag aggaaatagc taactgatcc tttttaatat
    37921 catgttggat cgctaaaaac atgtcagctg cttcttgagg gaatccggct tcttttagat
    37981 tttcgacaaa cgctgcatca cttgcttcaa taatttctaa ctttttacct gtcgcacgct
    38041 ctaatgcctt ggctaattct tcatagtcag ttagattgcc ggataattct aagatttctg
    38101 gagagtcact ttctactagt acttttgctg cagcttctgc atattcacgt ttcaatgccc
    38161 aacctgtttt tccatttcct gcagcataaa cgaattgacc agtttttaaa gcctgcccaa
    38221 tgataggtaa ttcattctct acataccaat tattccgtaa aaatgtatgg gaaatacctg
    38281 aatccttaat cattttttca gtgaattggt gatctggcgc taatacactg atcgagtgat
    38341 cggcatctgc aaaacttgta taagcgatat aagatacccc agctgcttgt gctgccttca
    38401 ctacattctt atgttcggct tgacgatttc ctggaacgcc agagacaaaa agcaatcgat
    38461 ctattccttt caacgcttct ttcattgagt ctaaatcatc ataatcacca atacgtatat
    38521 taaatccagc ttctttcagt gcttctcctt tttcttcact acgtactagt acatagattt
    38581 cattttgtgg aaccatcttt tttaagaatt ccagcgcata accaccaaaa cctccagttg
    38641 ctcctgttac taaataatgc atatttgcca ctccctattc tttttatttg ttaagattta
    38701 cagctgtaca tttataaatt acacctttat cataaagagt gtggtaagat aagtcaagca
    38761 aaaaaaatga aaggatgaca actgatgaag tattctctgc aatttagtga tgcaatccat
    38821 gtacttgcct atattgaaat gtttcaagat acccgatcac tttctagtga gatgattgcc
    38881 agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc
    38941 ggtttgatct ttacaaaaac cggaaaaccg cagccttccc ttgccaaaag tccagatcag
    39001 atttctttac tagatgtcta tcgcagcatc gaaggtaata cacagcttat ccaagtcgat
    39061 ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc
    39121 tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta agatcacact tgatagtctg
    39181 atacataaaa tagccgtttt ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag
    39241 agatttttat aattctctct actgtgcctg catttttctg atcggaatcg tctacataaa
    39301 ttcttttatt ctccggggga caaaagcctt gtcatgcccc tttttcctct ctaatgctca
    39361 ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt tcaagtagct gaccctcggc
    39421 attagttctg aaaaatgcaa aatatggaaa gctatttccc atttttcctc tctaatgctc
    39481 aggtcaaagc gctactttca cgacctctat attatcatgg tttattgact accggtatcc
    39541 agatttctcc catatatttc ccgttggtaa ctcccatttc gactacagca tttggacccc
    39601 caacataggc gtaatcttct acttctctta aaacatgccc aaaggccgta ttcgtcaaat
    39661 catttcttaa ttcttcaggc gattctgcta aagcgattac aacaagatac gttccttttg
    39721 ggaatgggat gattcgggta gtgtttggaa catcctgact cgtttgaaca ccgacatagt
    39781 acatcatttt cccgttatag ggttcgttga ctgtaaagaa ataatgattt tcagcagctt
    39841 gtttcaatgc atctattgtt ccattaccga taactttttg ctcaaatgct tgtttctctg
    39901 cagccaaacc gacaaaatcc gtattgctac tcttcaattc cacaccaata cccgtcaccg
    39961 taaaatctgg tttttcttcg atcctatact ttgccatttc tcccaccctt tctattatat
    40021 taagttcatc ttacataaaa ttaaagggaa atgacacgat tacgctcttt tttctaggga
    40081 tatttttatt ttttaactat ttttttgctt tcactgcaat ccaaatctca ctgtaaacat
    40141 tactgaaatc ctctccccat tttgtaaaag agatctcggg agcttcgact aactcataat
    40201 ctgaagaagg aagccattct gcatagattt tcccccaagt atcttgtaaa gtttggggaa
    40261 atggcccttt attaggaaaa atggcccaag tgtgttcttc tatagcaagc atttctaaat
    40321 cagcgtacgg attttctttt gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt
    40381 cttccaagcg ggctccatca aatccataag aaacgttgat tacttgattt ggatataaat
    40441 ctcctaactt atgcatctct tccctttgag tctccgtgat cgtctgcgct aacttttgta
    40501 tttcttgatt gactccttca aatttgacag gaactttttt cgccacacct gccacataaa
    40561 actgttcttt cttttcgata cgaaattcca tcgattttcc tcctctgaca tcaataaaaa
    40621 atgtcaagcg tggaaaagat ttttgtatgc cgtatctgct gatttctgaa ggcagatatc
    40681 ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt ttcatatcca tatctaaacg
    40741 ccgtttctgt cactcttgat ccatctagga gatcgcgatt agccaaagcc aatctacggt
    40801 ttttgatata gtccattagc gacatccctg caataaacgt aaagatgcgt tttaattgat
    40861 attcagagat tcctgtattt tcggctatta ttttatagga aagtccttct gtcaaatgtt
    40921 cttcgatgta atcgatcaat tggttcagat ctttaagcat atttatcttc ctccttgctt
    40981 gcctttttat catattatca aagaaagtca attacttgat gaatcaggaa ccaatttagt
    41041 cagatgtaga aaatgttgta tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga
    41101 taattggtat tatctgccta tctatgtttt acctttagaa aaataaattt tggggaagac
    41161 acattctaag taaacaaagc ggtaaaaata tcttttattc ccttagttaa tccgcttact
    41221 acctactgta caaagcaaat tgttgttcaa actgctgatc agaacgctgc ttggattttt
    41281 ctttgcgata catcatacct cttttatcaa ttggaacaat tatttatttc taacaaaatc
    41341 acttcatttt tgtcagataa ataaaaaaca aaaaaagttc catctttaaa acacgctatt
    41401 cttttacttt ttctcacatt ctctaagaaa agaaaaggag aatgaatttt tgacaattcg
    41461 agcaaaagtg attatcaaat atgtaaaatc agctactttt ttcttaaacg tttctttata
    41521 ctaaatataa ataagtgttt catgaaagga gaactagatg aataattggc ttgagtattt
    41581 tcctgaaaac gttttggaac gcggttacag ctatcatctg cacggttttg tccgtcattt
    41641 gaactataca agcaagtact tgtccgcaac agtttctggc acggaagatt ataaggtagt
    41701 gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa
    41761 aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc
    41821 caactactca ctttccgaac tctcgtccct tgtttcctcc gctagttcct ctcttgtacg
    41881 ggagctttta acagagattc ttatagaaca tccgcaattt atcgaacgct tcaaagttaa
    41941 gatgccattt catgcaataa attattcaga taaattaaca actatcatcc ataaatacga
    42001 tcatattatc aagaaaaaca aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa
    42061 gttcatccaa gaagcagttg aatcgctcat tcagcaaaat gcctatcttc ccgcattcga
    42121 attgatcaat gaagtgattg ctactctcga aacattctat tgggagcctg aggatgaacg
    42181 aacccttctt ctgatagagg actgttatta tttatggaaa gaattattag cagaagcacc
    42241 tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc caagtcgatc atactgacgc
    42301 ctcttattca aaacgctact ccataaaaat cctgaaagaa gactttcggg aaaaagaatt
    42361 ttctaaccaa aagaaaaaaa tagataaaaa aactaaagaa acggtaaaga aagacgattt
    42421 caatgaaaaa tagcaagtaa acgataacaa ccatcctaaa tccacaagaa atacagcaga
    42481 ccttgcttta aaaacacgaa tcatgcaaag gatgaagtaa agaagactta acgcgtaccc
    42541 aatattagtc atttgactat taggtacgcg ttttgtttta taagtcatga caaatttcct
    42601 tcgttgctgg gtagattttc cggcattgtt gatagatttt atgatacttt tctacatttt
    42661 gtaagagagg ttccactgca tctgtatatt ggataaaatt ctgacaacaa ctttcaggtg
    42721 tgtcgaacca ctctaatctt taaagataaa tattaaaata acgattaata atattaacag
    42781 tctttatttt tcaccaccaa aaacctatct ttgcatatac taaatagacg tatatacaca
    42841 gataggtttg tcttgtgaaa aataatgatt atccttacca aaaaatgttt gtttttctac
    42901 atgttcgtac ttatcaaaaa atatgatcct tattgaaatt actggctatt tatattccaa
    42961 cgacttcttg tgttaatttt tgttatctca tagtaaatta tgtttcattt tcacttaatt
    43021 tagttgtcac gtgttcgtca attcgtaaaa acggtaaata tataaacaca cttgcggcta
    43081 taattaatat ttgtaaaaca gctgctcgcc agtctccggc tgttgctaaa aatcccgata
    43141 taactggtgg cgtagtccaa ggaatttgca ctactacatg attaattaat cccacagcag
    43201 ttgcaaaata tgctactgtc aaagaaaaaa tcggtgcaag cacaaaggga ataatcagac
    43261 tgatattaaa tacaataggc aatccaaaaa taataggttc gcttatattg aatatagcgg
    43321 gagttaccga taatttagca aagtccttat aatcttttct acgactgaag ataaatattg
    43381 caatcaataa agcaatcgta tttcccgatc cacccattac cgcaaaggta tctttaaagg
    43441 ccatattgat aatatgtgga atttgctctt tgtttgcata agctgccata ttttcttgca
    43501 tattctgaat caaaaacgga tctaacaatg aacctgatat tactgcttga tggataccaa
    43561 atccaaaaaa tagattagca atagatgtaa ttaacaaaaa tcctggtaaa ccagttgtaa
    43621 ttttacttaa tggctctgta actatcgtgg taattagctt atttatatcc atattaaaca
    43681 attgaatgat tataaatgac aataaagcaa acgaggtaat cgtaataatg attgggatta
    43741 atacattaaa agactttaaa acagcgggag gaatatttcc ggatagctga atctgtagtt
    43801 tcttattatt agaaagttta ataaataatt ctgtagccgc taatccaaca aatataccta
    43861 caaacattcc tgaagctcct gtataggata taggtatcac acccgtcact tcaacagctt
    43921 tatccgcatt ttcaggagta aacatcatct gcataggtgt taatacaaca aatgtcgaaa
    43981 tagcaactaa taccgctgca atatgatttg tataatttct gcttgcacac atatgataag
    44041 atactgctgc tacaactaat aatgtagtta tgcttaatgt accattggca attgaagtac
    44101 caatttgttg taacgtggtc aatgtactag tatcgacaat ttcccccatg aatcctgttg
    44161 gttctaagat cactgaatta ataagtgtca caaatcctgc taacatcata aatggcataa
    44221 atgtagcaaa cgcatctcgc aaagttctta aatggatctg attccccaac ttagtcgcaa
    44281 ctataccaaa tttttcaaat acaatgtcct tcctgctact tttgcccata ttttttctcc
    44341 tttatgctta ttgtaatcga ttactttttc cacccaaata atacatcact tatcaaaaaa
    44401 agtaaatact tttaattaaa tatatatatc ttttttagta taaaaaaata agctctgaaa
    44461 aaataatttt cccgagctta tttttcaaca aaaacagctt caaaaacaaa gtcttgatat
    44521 aaatgtctcg ataccgaata ctcgaaaggt ttaccagacg atagagcaac aactttgtct
    44581 atctcaatca taaattcacc tggatctatt tttaatattt ttgcgtcaaa ttcattcgca
    44641 gtatctcctt tgatacgtat gtgcgaagtc tttatttcca gacccagttc atttgtgata
    44701 taagcatata cagatttctt taaatgtttt ggttccaagc ctggtatgac tgctaatggc
    44761 atgaatgttt gctcaatcga gtatggttga caatcaataa aacggcttcg tacaatatta
    44821 tatacatagt cttttacact gatacttaat ttatcagcta cttttttcga tggaagttca
    44881 attgagaaag tatggatttc tgaatctacg tgttcttcac caaccacgct aaacaaacct
    44941 tcgtttggac caagaactcg tgagttatca attggtgttc tgacgacttc agtcccatac
    45001 ccacttctac ttcgcaaaac tccttcggaa acaagtaagt ctacgccttt ctttactgtt
    45061 aacttgcttg tatgataaat aactgccaat tcatctcctg taggaagttt ctcaccaatt
    45121 ttatactctc catttacaat ttttttcttt atatctaagt aaatttccat atacttcgca
    45181 atttttttca atggtgaccc tccaatcaaa aacatatctt catctatact ttaacataaa
    45241 taaaccgata aaatccacat caaaattata cacacaataa aaaagatata tatatttaaa
    45301 caaaactatt tactttaagt tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa
    45361 aaattatgac aactatgaaa aagctaactt ttccagatga tttttggtgg ggttccgctt
    45421 ggtcagctga gcaagcagaa ggaaaaggag aaacaggaaa agcagagact atctgggatc
    45481 gctggtttaa agaacaacct taccggtttt atcaacgaat cgattcctct attacaaccg
    45541 atcacatcca tcaatataaa gaagatatag aactaatgaa aaaaactggg cataactcct
    45601 ttcgcgtttc tatttcttgg gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc
    45661 ccaaagccat ttctttctat cgaaaattat tttccgaaat gaataataac ggaatcaaag
    45721 tatttgccaa cttatatcat tttgacatgc ctgctactct tcaagataaa ggaggatggg
    45781 aatcacgtga agtagttgat gcttatgttc attacgctga aacttgtttt aaagaatttg
    45841 gtgacttagt ttgtcattgg tttaccttca atgaaccact tgggcctatt ttaggtactt
    45901 atcttgaaga ttttcattat ccaaacctca ttgactttaa acgcggagcg caagcagcat
    45961 tcttcactat acttgcacac gcaaaagcaa tacaagtatt taaagaacta gctcttccta
    46021 gtaaaatagg cgttattcta aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg
    46081 atgtgaaagc agcagaaatc gctgatttat tttatacgcg gagcttttta gatcctatgg
    46141 ttaaagggaa tttttctaaa aagcttatcc tgttattaca agaatataat cagcttcctg
    46201 aagattatac gaaagaagat cttgtatgta tctacgaaaa taccgctcaa attcttggac
    46261 taaactatta cgagccacgt cgcgtcaaat ctcgttcttc tatggtggat acatctagtc
    46321 catttttacc agagtggttt ttcgaaccac atatcatgcc tggacgacga atgaacgaat
    46381 accggggatg ggaaatttac gaaaaaggaa tttatgattt gtgcatggat atcaagaacc
    46441 attatggaaa catcgaagca tttatctcgg agaacggaat gggcgttgcg gatgaagaac
    46501 gtttcatgga tgatacaggg caaataattg atgattatcg gattgagtat atcaaagatc
    46561 atcttgctta cttatggaag gcaataaatg aaggatgtaa cattaagggg tatcatcttt
    46621 ggacatttat tgattgctgg tcatggatca acgcctataa aaaccgatat gggctggttt
    46681 cactcgacct tactactcaa aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat
    46741 taagtgatac aaatggattt agatacgatg tagacttgtt agttgaataa tattctaaaa
    46801 ttccacttga agtgaacaca ttagaaaaca agaataatga gaaataccaa taacaaatac
    46861 tttttcttca tacttcattt atacttgtca gtataagaga ctgtgacaaa tgtagtgatc
    46921 ctcagaagtt aggtctggaa atctaacttg agaagatttt tttaaactac gtccagtctt
    46981 ttttatttgt ttaaaattga agttgacaag tcttcattaa aaatttagaa tattcacaag
    47041 tttgacgaca attatgaaaa tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag
    47101 ttagatataa aaactgcgat caaagacacg ttacctaccg tttttggtta tatcggtatt
    47161 ggacttgcat ttggtatcgt tgggaaagct gccgggtttc atccgctggt cgtcacgttg
    47221 atgtccctgc tgatctatgc tggttctgcc caatttatca cggtcagcat gcttgctagc
    47281 catagtccac tgctctccat cgttctctcg acctttctag tcaattcccg aatgattctg
    47341 atgagtatga cgattgctcc ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt
    47401 ggcacattgc tgacagatga aagtttcgca ctgggaatga ataaattgaa ctatactgga
    47461 caaaagttaa actttcgttg gatgaataca gctaattgga tttcttattt gacgtgggtg
    47521 ggttcttctc ttgttggcgc acttttaggg aattttatta ctgatccgaa aaagtttggt
    47581 ttggatttcg ccatcgtcgc tatgtttatc gggttacttt atcttcaagt catttctgat
    47641 agaaacacct ctaaacgatt acagttgatc ctgattggtc tcactttgat tctggtatat
    47701 gtcggactga tttttatccc aagtaactta gtgattttag ttgtaacact gattgggtgc
    47761 ggattggggg tgtggatcaa acatgccttc ttttgagttt acattgctga ctattttagg
    47821 ttgtacttta gcaacttggc tttctaggat cctaccattt gtcttactga aaaaaatcga
    47881 tttgccgcaa ccgttgcttg agtatttaag ttttgtcccg atcgtcatta tgtctgcttt
    47941 atggttcagc agtctattta cccaaaatat cggacatctg ccgcagatca atctcgaaaa
    48001 tgctttggct tcagttccta cacttttagc tgctattctt tcaaaaagct tattggtgat
    48061 cgtcttggta ggaattcttt ccctttcgtt gattcgctta ttataaataa aaaaacaaga
    48121 gcttatggca aattactgtc atgcgctctt gtttttatca aagtcaaata agaaaaaaat
    48181 cggcgtattc ttgcttatcc gttgaacgga ccactaacaa cgacttttcc taatatatgt
    48241 cctgtttcta cgtctttggt tgcttgtttc aagttcttcg cattcatccc atctgaatag
    48301 accttgctca atgtactgcg gataatccct tcatttgcta aagcagcaat gtgcgctaaa
    48361 gcttctcctt gcgtttcgat ttcatagtta tagtctgttt tagcgaacat atactcccag
    48421 tcgaagctga tagattggtt tttccaaaca cctatatcta acggctcttt gatccctacg
    48481 atcgttccca catgtccaaa tggtcggaca aggtgtttga tttgatcaaa gtagcgggta
    48541 atatcgaata atacagcgac atactctact tgatcgattc ctaattcacg aaaactttta
    48601 cttaagtcgt gatgataatc gattggatag tctacacctg tttttttcag ccattcaaaa
    48661 ttcttcggac ttgcagtcgc gtagacttct aggccagccc aatgcgctag ttgattcaaa
    48721 atcgaaccga cgcccccagc tccgttgatc actaagattt ttttcccgcg gttggcattt
    48781 tcttctggag tcagaccaaa tttttcaaat aacaattcat aagcggtcaa tgaggtcaac
    48841 ggcaaggctg cggcttcttc atctgaaaga ttttttggtg caagagcgac aatacgttcg
    48901 tcaaccagtt ggtattcttg attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg
    48961 cgatcgccga cagaaaattt ttgcacttca tcacctacag caacaacttt cccgactcca
    49021 tcaaacccta agatacgtaa agcatttctt atcccgttat cttggcgaag tttcgtatcg
    49081 acaggattaa cagatactgc gttgactttt accagaagat cccgtgaact gggtacaggt
    49141 atctgttctt tttcatcaat aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa
    49201 ccgactacct tcattaattc cttttctgac atacgaattc ctccttagct tcatcaagtt
    49261 ggagcatcca tacagttttc atttcaatat atatagtatg ctatttgttc cacttttatt
    49321 catcataatt attctcttac ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg
    49381 ttgcgacaag acttttgtca caacctcttt taaacggtgt tcaatcaact gaccctcggt
    49441 attcgctcaa aaaacgacaa aatatgagga gctatttttg tttggctgtt cttattactt
    49501 gatgcaggac agctttttca cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt
    49561 cactgaaaag cgttggatgt tctttttcaa aggtgtctaa gatctctcgt acttcatcag
    49621 tagatttcgt attcatcaac tgaactcgca agtcatttgc tcctggaaat cctttgatat
    49681 agattttaaa gaaacgatgt agtcctgtga tggaacgcgg gatgatttct gaataatgat
    49741 cttgcagatc tagttgcaga cgaagtagtc cgatcagttc tttttctgta tgttccctcg
    49801 gttctttttc aaatgcataa ggattcttga aaatcccccg gccaatcatg atgccatcta
    49861 cgccatattt ttcggccaat tcttctcctg tctggcgatc tgggatatct ccgttgatcg
    49921 tgatcagtgt ttgcggagcg atttggtcac gtaaagcaat gattttaggg atgacttccc
    49981 aatgtgcatc tactttactc atctctttac gcgtacgcaa atggacagaa agattggcta
    50041 tatcttgatg aagtaggtga ctgatccatt cttccatctc agacatttcc gtatagccga
    50101 tacgcgtctt gacgctgaca ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg
    50161 ctacttctgg gcgaaggatc aagccactgc cttttccacg ttctgctaca ttagggactg
    50221 ggcagcccat gttgatatcg attccttgga agcccatttc tgccatagca atgctcattt
    50281 ctctaaaaaa ttctggcttg tctccccaaa tatgggcaac catcggctgt tcatcttctg
    50341 taaaggtcaa tcttcctcgt acactgtctt ttccatctgg atgacagaaa ctgtctgagt
    50401 ttgtaaactc agtaaagaag acatccggtg ctgctgcatg ctttacgaca tggcgaaata
    50461 caacatctgt tacatcttcc attggtgcta aaacaaaaaa aggctttggc agctctttcc
    50521 aaaaattagt agtcatttat ttgttactct ccattctatc aagcagtgaa gtcgcaatct
    50581 ttctcaaaaa cattgtcatt atactaggaa acaaaaaaag aagcaaagtg aacagcttaa
    50641 aagatgtttc tgattgagca aaaaaagact gtgacaaaaa tcttatcaca attccttttc
    50701 cggaataaat ggtgttcaaa agcttgcatc tgcggtaata agtccaacca aacgaaaaat
    50761 aggaggagct atttttgttt ggctgttctt attacttgat gcagaacagc tttttcacaa
    50821 cctctccact aaacggtgtt caatcagctg accctcggta ttagctcaac aaacgacaaa
    50881 atatgaggaa ctattttccc gtttgtttct ctaatactca ggtcaaaacg ctgatttcac
    50941 aacctctttt ctaacttctc aatcctcttc ctttattgat caaataccaa gtaacggcgt
    51001 acaaaacaac gatgaatagc acaagtacga tcatcgatgt tgctactggc acatcgattg
    51061 ttccaaggaa tccgtaacgg aaaccgaaga tcatataaac gatcggattg actttcgaaa
    51121 cagcttgcca aaatggcggc aacatagaga tggcatagaa aacgccacct agataagtta
    51181 acggctgcaa gacaaatgta ggcacgatcg atacatcgtc ataagactga gcaaaaatcc
    51241 cgttgagcag tcctgctaac gaaaacaaga ttgccgtcat caataatgta atgatgacca
    51301 tgaaccatga atacacatga agtggtacaa agaacaagga aatgatcgtt acgagagagc
    51361 cgacgagaat actgcgaccc actccgccaa tcacaaagcc ccaaatgatc acatgcgtag
    51421 gaacaggcgc taccaagatt tcttcgatgt ttttctggaa tttttgagag aagaaggacg
    51481 aagaaacgtt ggcatacgaa ctagtgatag cagacatcat aatcaaacca ggcacgatga
    51541 attccatata tgaaaaacca cccatgtcac cgatccgacc accgatcatt tttccgaaaa
    51601 taacaaagta caaggatgtc gtgatcacag gcggaaccag tgtttggacc cagatacgca
    51661 aatagcggtt cgtttccttg atagctaaac tttttaatgc agtaaaataa agattaaaca
    51721 tttttctcct ccgtatgatt attttcttct gtgattttca aaaacagttc ttctaaacga
    51781 ttcgacttat tcctcataga aagaactttg attccttgtc ttgtcagctg atcgaagatt
    51841 ccgttcaccc cttgatttcg ttcaacttcg acagcgattg tctgttcatc ttcaaatgta
    51901 caagcgtatc cttcaatcac aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt
    51961 tcaaattgca gtttggatag aagctctttc atactagtat tctcgatcag ttcacctgat
    52021 tggatgatcc cgatgttgcg acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa
    52081 atgatcgttg taccattttc gttcaattct cggagaaact cccacatctc gcgtctcaat
    52141 tcaatatcga ctcctgctgt tggttcatcc aagatcagca aacgtggttc atgcatcaat
    52201 gccctagcaa tcatcagtct tcgcttcatt cccccggaaa gcatacgtgc acggacattt
    52261 ctcttttccc aaagattgga ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg
    52321 cgagagacac cgtagtagcc tgcttgattc acgacgattt gctggaccgt ttcaaaggga
    52381 ttgaaattga actcttgcgg gactaatcca atctgttgct ttgctcggac tagatccgta
    52441 tccagatcat aaccgaagac attcactttt ccagatgttt tattgactaa agaagtaatg
    52501 attccgatcg ttgtagactt cccagctcca tttggtccta atagcgcata gaaatctcct
    52561 tctttcacgg aaagatcgat tcctcgcaat gcttcgaccc cacttccata aacctttttc
    52621 aaattattta tttcaagcgc atttgccatt tatttcctat ctccttatag acatgattct
    52681 ttattgtatc atggaataca gcagatgaaa aaacaacttg gttccagcga aaaatcaggt
    52741 tgctgggctc aggaaaatct cctaatgaaa gacatcaatc ggacgaagtc agacaacctt
    52801 ttcagaaaaa acaaatcgaa gataaaggat tactattcat atgtgcaaca aaaggagcgt
    52861 gagacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
    52921 aaagccaaga tttttgatcc aataaatact ttcgatcact tcgcctatcc caatacatgc
    52981 cactgccgct tttcatcaaa taacgcggct tccgtttttg cggtttctac taaagtctct
    53041 gggtaatcca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
    53101 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
    53161 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
    53221 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
    53281 tttgtccctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
    53341 tccaacaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
    53401 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
    53461 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
    53521 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gaccggattc
    53581 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
    53641 aactctggtc ggcaagtgat gggcatataa gtacggtaat tcaatattgc agcggctact
    53701 tctaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
    53761 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
    53821 atcaactctg cttcactccc actcttcata cgaaatgaaa aacctaattt agggatgtct
    53881 cttaatggtt tagtttgctg tttcagctct tcttgaagtg gtgtcggcag ttttgctagc
    53941 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
    54001 gtttgtttct tcactagata atagaccgta ttgaatacta acccaccgat tgccaaatag
    54061 attcctagta cttgtcctaa aagaagcaaa cccaaaccca ccagcggaag caagccaaga
    54121 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
    54181 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggatttt ttctctcagt
    54241 tgaggatttt cctgataaaa agcaatcagt ttttctaact gctgatcttc accaaaatca
    54301 aagctgcgta aacgttgata caacgcttct gaacctacac tagaataggt actgttgatt
    54361 ccttcgaata cttcgaacat atccaggtca taccaagtaa gatcatcgat ttcgctatcc
    54421 catgaacgga acttcttctc cgtgagccat gcttccttca agctttcttc tttatcaaat
    54481 ctcggttgat aaggaatttt ttcccatttg ctacgaactg tttcctttaa tttcaaacgt
    54541 gaaaaaatgt ctaaagcaac taataaaata acagctgcaa taataattaa aacgataaat
    54601 tctctttgac ccatatccct ctccgttcct ttatttgtta gctttagtat agcactgttg
    54661 cgctatttga aaagagggta aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta
    54721 tattacatat ttatctctca tttatcaaaa ttgacaaata atcataggga ggaattgttg
    54781 ttaattcttg tttattctcc agtgcattga caactgtccc aagggaaagg atagcctcag
    54841 gtactatttt ttcttgttct gttggattag ctaagcgaag aacaaatcct tgttcatgaa
    54901 aataagaagg ataaatcgat gatacaatta agtcttcagg taattcaatc aatgaaaatt
    54961 tacgaggaac atcaagttca ttttgtaatg gccaaatctt gttatctaac ctgtgaataa
    55021 atacattcag tttctgcttt tgataagaaa tagattgttc taaccgtttt tctgcctgtt
    55081 ttgccacttt gaactcatta aatcttcctt cttctacatt aaatccaaaa gtgactttat
    55141 actcacctat ctcttgagct agcggtgttt ccatcatgac atgcccttca tttgtcgtat
    55201 ctcctgaagc tcgcccaggt cgccaagcaa gattaggttt tccaagttcc cctgttgttg
    55261 aaaataaggt tatgattaat tgattttcta tacgttcata ctcttttaaa ccatctgcat
    55321 aaaacgtaaa ataatgatca ttttctgcca cagagacact cttatcgaat atttctaaat
    55381 taacaggttt ctctaccata gtttctgacc aattcggagg aatgacatca ggggtatttt
    55441 caataaatcc gttttgaatt tgtgcaattg atttttcaga tttatctaaa acattaattg
    55501 ctaagcgcat acgatgcgag taaacagtat tatgaatttt gagtgtgcct tcaacgattt
    55561 gactttctcc cagactgaac gatatttcat aagatatttc ttccatttcg ggtttttcag
    55621 ataaacgatc ttttaaatct ttaggaagtt gagcttttcc gtaaacaact aatgtttctt
    55681 gtaaagaatc tttatatact ttagctgttt ctaattttag agatagttct gtatctcctt
    55741 ctaatggtga ataatcatac gtatcgccat catttgctga atcgatcaaa tgaacgaaat
    55801 caagatattg tctatctcca actatcaaat tcaattgccc atctttaaaa gaaagtttat
    55861 atttattgtt acttatctca gttttgtcta tctcgattgc tgtttctaat ttttcattat
    55921 ttttctcaaa ttcaacaaca gtgtatccta gtgctggcac atttacattt atttttatgt
    55981 caagttcata ataagctggt tcatcaaaat attcgaaacc tgtagcaatc atccgataag
    56041 catttttccg ttctggaaaa taaacttctt tttcaatgat attataggga ctacctttga
    56101 agtgtatttt ctttgaatca gctactacat gcacgatttt cgatccacta taattctcag
    56161 tcgttgtgtt gaaaacaagg acttcattcg attttagctt taatgcatca gccatccgtt
    56221 ttacaataag gttttcgatt ccgtcagcta tctcattagc ttctttgatt cgatggaaaa
    56281 tatcttccgc aacattatct gaaacacagc caccaatact atcatgagcc tggttttgca
    56341 aaatcttttt ccataaccga cgaagtaggc ctttactgat atggatacca tttctttctg
    56401 caatcagcat gagcggctca actcgtttta gaattttttg ttccaattta aaattttcta
    56461 ttttcatttt tgttcttact gagccaatag aacgatgaac acgagagtaa accggctgac
    56521 gaagttcacc tttatattca ggtaattctt ttttgtttct gataacttct acataagaag
    56581 gataatctga aataatattg tggaatttac tgtatttatt caacttctct acgacttgat
    56641 caaaatttaa aatcattgac ttttgatcta cacccgaagg tactaaaata tctttgtttt
    56701 ttccttgcat atgaagcatt tcaactgctt catccaactt ttcttttgca aattcaggaa
    56761 tatctttaat tgcgtctaag gtcatcattc cagtcatata accaaatgga aaattcatgg
    56821 cataaacaaa tttatctccc aatcctttcc agataaaata aggagatgga accatcgttt
    56881 caaagtttaa tcctcgccaa cacatgaaat tatcgatttc tacttgatgc aaaagtgtag
    56941 gcaaattcgc attgaaccca aacgtgtcag gcaaataccc taccatcatt ggatctccat
    57001 atttaaggat cgtatcactg attccaatca ttaagtttct taaaattgat tcagcatcca
    57061 ctaataaggc atctgtttgt gcataccacg gtcccacgaa tagttgtcct gttttcatca
    57121 gtcgttggat aaccttaagc ttttgcggat tgatctccaa atactcatcg acgattgaag
    57181 tttgtccatc taaacaaaag tttactttag ggttcctttc caattcatct agaacttcag
    57241 taaagacctg atcagacaat acgatggcat cttcatttgt aaaataccat tctctgtccc
    57301 agtgcgtgtg ttgaattata tgtgcattta ccatatcaga ttctcctact ttccttcttc
    57361 tactgaattt acttgttgtt cttttttata cttgatggat tttagtgcat tgaccattaa
    57421 cgctgtaacc actcccccaa taacaattga tactaaatat aaaggtatat tcgtaacaaa
    57481 aggaattgcc ataaaggttg aaagcattgt ttcttgagta acaccaaaaa atccatttag
    57541 accacatgct actgcagtgc ccaccacgat gcttggtatt actcgtaaag gatctttcac
    57601 ggcaaacggg atcgtccctt cagtaatccc cacaaggccc ataatcatag ctgtctttgc
    57661 attatctcgt tccgattcat cataaagttt tttgtttaaa attgttgcta aaaacattcc
    57721 aaatggcgga atagcaattc ctaataacat ggctgtacta ggtccaaagc ttccgcttga
    57781 aaaagaagca accccaaaag catatgcgat tttattgata ggtcctccca tatcaaacgc
    57841 catcattcct cctaaaatag ccgacatcaa agcagcattt gctcctgaca tgccttctag
    57901 ccaattagtc agtcctgttg tcattgcttg aataggcgta gctagtacat accataatat
    57961 gattccaaca actaatgtag taacaaaagg aatcagcata attgatttta gactaataac
    58021 ttgcatcggt acttttactt ttttcaatgc attagctaaa tagccacaag ctaatccagc
    58081 aaccattcct ccaataaatc ccataccaag attttgacac atcatcccag tgataaaagc
    58141 tggtgctaga ccgggacggt cagcaattga atatgcgata taggctgaaa tcatcggtac
    58201 gataaactgt aatcccgtct gaccaatcat tcgcaggact ccccagaatg tatccgtctg
    58261 gtcccaaaca cctgtgccac caaaaatgac tgcaatagaa gttagtaacc ctgctgctac
    58321 gacaagcggg atcatatagg aaaccccact ttggaaatga tttagaattt ctcttccttg
    58381 tttcttgaat tccattagta actcccccac gatatatttt ttatttgtta attaatgcaa
    58441 ttgcttcatc aatcgtctct tcaccatctt ttactacctc tgaggtccct actgttaaaa
    58501 tcggtttacc gtcaaagcga cctcgttctg aaacatttgt atctactgca aatatgacaa
    58561 catctgcctc ggaaacatcc gaaattgaaa gtttgttttc tatccccata gccccctgtg
    58621 tttctacttt aatatctaac cctttcttct tcgcaaactt ttctaaatta gcttttgcca
    58681 tataagtatg ggcaatccct gcgatacatg ctgttactgc tactattttt gtcatatgaa
    58741 attcctcctt ttaaaatgat agagctttta ccaattcttt tttatttctc gaatctttta
    58801 ttcttttaac aaactcgtca tccatcaatc ttccagataa ttctgctaaa atacgtaaat
    58861 gattagtacc tttatcagtg tcttttattg ctaacaaaaa gacgagatca actggttgat
    58921 catctaatgc gccccattca acagaccgct ttaacttggc tacagccact gttgattcaa
    58981 ttactgctag gcttttgcca tgaggaatgg cgatattttt cccaattcct gtggtcatat
    59041 ggttttctct gtcaaatacg tctttttgaa attgtttgtg gtcagacaca aacccattag
    59101 tctctaacaa tttcgataat ttttcgacta cacttttttt atcagatgca tttaaatcta
    59161 tcactatatt atttatattg attttttcac tgatcattta taagccttct ttcaatatat
    59221 aaactaattt ttcaaacgac gaactcgttt ttaaatttgc taataactca tgattcgaag
    59281 cgatttcatt gaatgtttca taaattttat tcaattcagg ttttactttt tcattaagtg
    59341 caaaaaagaa aacagaatga acaagtgtcc catcccaatc aattccttct ggattaacgt
    59401 agacacagat tactggcttt tttataaaat ctacttcgat atgcggcata gcaaaatcat
    59461 tcatcgctgt tgaagatagt ttttctcttt tgattgctcc ctctagtatc ccttctttcg
    59521 cgtaattttc ttttaccaat tttttggtaa ttatatttaa aacgttcact tgattatcct
    59581 ttatttggtg ggtaaaaaag caaaatcgtt tatctaataa atttagaaaa gcatcttctg
    59641 tttgttcatt taactgtaga tactgtttta cttttctgac atctgcccct aacatcaaag
    59701 gactaacaac aataacagga aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt
    59761 catcagtgac ttttctatct tgtagttcat tgatgcttag tacatcttct atctcaacca
    59821 tatctgcaaa accgttattt attctttgct ctaataattt tgatgttcca tatccgctac
    59881 tacaaactaa tatcactttc gttttgtcag gtttgtaacg atccaaaagt gattgaatat
    59941 ggagtgttac gtaagcgatt tcatcttcat taaaatgtat acagaatctc tcttcgattt
    60001 cctctaataa atctacactt atcataaatg accttttaaa agaatatttg attttgtccg
    60061 tataagggtt atagatattt actcccaatt tgagtctttt aactgctgca ttgagatgta
    60121 atactagatt attgatcaat tcttcatcag gataaaattc atttaattta attcttacta
    60181 actcttgtag ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat
    60241 tcatagcatt tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg
    60301 gcacaataat tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga
    60361 tttcttcttc agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg
    60421 caatagcaag gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct
    60481 tattcatgta tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat
    60541 tattcgcttc accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg
    60601 ctgtaaagcc accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt
    60661 catcagcttt gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta
    60721 gtctttttct aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt
    60781 cagcaagatt ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag
    60841 ttagacggtc ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa
    60901 gtgtttcttt ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat
    60961 ctggcatttt ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag
    61021 atacatataa ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt
    61081 atagtatttt catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac
    61141 agcttaagtg tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta
    61201 gaagtttcta atattcttga ttatttcaaa tcaataaaaa agcctgcaat atttatatca
    61261 caggcttttt tctctattaa atatatataa gataatacat ttttctacta gttctcacta
    61321 atcttgtata attaaaccct ccgcaacttg acggagatgg agaagatgcc cccgtctaat
    61381 ctagcggtca tctctcctcc cattccttcc accaattttt ttgacaggta aagcccgatt
    61441 ccgctagatt ccgtatttgt gcggctggta ttttcggtat aaaaacgctg gaagactttt
    61501 tcaacagagg tttgcgttcc tggtttgata ggattggcca ctgttaatcg aatgaatgga
    61561 tcctcttttg aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa attctgtatg
    61621 atattttgca gcactcgcag caacatgagc ggatctgcta atacagttgt atttatcgag
    61681 tgttcagcga acgctacctc gatatttttc tcagaaaatt gttcataaaa agagaaaaat
    61741 acttcttcca cctctttaga aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc
    61801 tctattcgag caagatcata aaactgatcc gtcatactga gtaatcttgc aacagattgt
    61861 ttgatgatcg ttaaatactt ctcccgtttc tccgtatcct ctacctgttc cagtaattgt
    61921 acataaccgt taatagcagt caaaggagtt ctgagatcat gagaaaggcc gacgatcgat
    61981 agtctgatgt tttcttccat ttcttgaaat gcttgtcctt tttgttcatt ttcatccaac
    62041 catgtattca gtgcatctac taggtccatt agattctttt ctcgaaattc gactgaaaga
    62101 cgaccaccat attcttgtcg ttcagaaaga gtggcgattt tcttagttaa tctcttgatt
    62161 tccctttgca ggaagaaaat aaaacttccc ataaagataa caacgagaag caacagccat
    62221 ttaatcaaat ctaaacccca ctccccacac cgtatgaata tacacagcat gtgcctttgc
    62281 tagcttccct cgaagattgg aaatatgcat attcactgtt ttctcactat caaaatatgg
    62341 ttcttgccaa actgtttcat aaagatttgc tcgcgaaaat acttttttcg gatgttctaa
    62401 aaaaagcttc agcagatcga attctcttcc tgtcaaatgg acagccacac cattcacatt
    62461 tacatccctc gactctttgt ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc
    62521 ttttacttgg acagaagtct gatgacgaag ttggatgccg attcgtaaca aaagttcttc
    62581 gatatcaaat ggtttcgtca catagtcatc cgctcctgct gccaacaagg aaactttatc
    62641 tctttgatcg ctttttgctg aaagaacgat gatcggaaca ttcgatgtct gtcggatttc
    62701 tgctaacaat gcttcacctg aaagccctgg aagcatgaga tctagaagga tcaaatcgac
    62761 aggatatgtg cttaacaata attttccttc cgtacctgaa taagcatcaa gaagtttgta
    62821 tctctgttca agaacctcct ccagtaagga atgaacagct tcatcgtctt cgataatcaa
    62881 aatagtttcc agcaggacca cctacttaat gtcagatttt tggaaaataa acattcctag
    62941 agctgatgat accacgattg ttactagact gaccagaatc attttcggtg cttgcgaata
    63001 agttgcttgt tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc actccgttag
    63061 aaattccaag cctttgattt ttgcgaaaac accgggaatg aaggccgttc ccatgatata
    63121 aagaaaagaa agaagcaaac tgacactggt acttctagtg atgaatgtca aagccactgg
    63181 aacagataat gtccctaatg tcagcagaag caatacagga aatactttta gaacataatt
    63241 tccatagttt ttgattccct caacttcacc aaatccgaaa gcaagtgtgt aagcaagcat
    63301 ggctagtccc gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt
    63361 cgacacataa taagtcattc gattccgtcc taaagataac gtattgcgaa tcgtaccgtt
    63421 ttgaaattct tccccaatat aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc
    63481 agccattttt tctaccatta aaatccctgt tgcaccgttg atgactgtat ccgttgcttg
    63541 gtttaattca ttgccgtatt ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg
    63601 aagtacgacc atcagtccta gtgagaccca cattttacgg ctcttccata cttttatttt
    63661 ttcagcagtt aatagattaa acatgtggat tgcctccagt taattgtaag aaataatctt
    63721 ccagtttttg cccaactagg tgaataccat ccacaggaac ttttgcttct accaatgctt
    63781 gattgatggc tgctacttgg tcaattcctt caaaaagctg gataacacgg ctagattgta
    63841 caaaataatc cctataacca agagagtcga gaaccgttac tgcttgttcc gtcgccgaag
    63901 tgtcaatttt tataaattga cgagattctt gtgccaattc ttctttcgat aattctttga
    63961 tcaagtgtcc ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat tcatctagca
    64021 agtggctaga aagcaagacg gtgatgcccc gttcttttac caaacgttgg atgatttctc
    64081 gcatttctgc catccctgct ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt
    64141 ctggattcgt gatcagtgca tttgcaatag ctaaacgctg gcgcatacct aaagagaaat
    64201 tctttgcttt ctttttccct gtattttcca atcgcatcat tttcagcagg tcttcgattt
    64261 ctctatccga cacaccgccg ttcgctgctt ggatacgtag gttatctctt gcagttaatt
    64321 ctggataaag agcaggggtt tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat
    64381 ccgtcgttcc aaataattcg atcgaacctt catcaatcga gatcaaaccc ataatagttc
    64441 tcataaaggt agacttaccc gctccatttt ccccaatcaa tccatagatc attccacgtt
    64501 tgatttcgat tgatacttta tctaatgctt tggcagcacc gtattttttg gttataccag
    64561 ttgctttcaa gaccgtttca tttccaaaat ccatacttat ccttctttct tattgatgag
    64621 tgaagtatag ttgacgggta taaagaaagc agccagaaat gtataaagaa agtgtaaatt
    64681 ttccaattga atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat tatcaggcag
    64741 tttgttatac gcttgtatca gtttcctgat tctttctttt cgaccaaagt cgtttctctg
    64801 tattcctctt cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa ttttaccaga
    64861 ccggttataa ggataactat agcaagactc cctcccagaa aaattagact gtctcgcaga
    64921 cgtggttgcg ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc aagtggactg
    64981 gccaccatgg taatcgttgt tatcgttgac ataagtcgtc cttgtatatg cgttggaact
    65041 tctcgctgaa gccagaccat ataaaaagca aaagaaaaca ttaatcctta aaaacggact
    65101 tatgaaaaat aatacatgcc gtttgggatt tacaattagc taccaaataa gaggttatga
    65161 caaaagactt ttcacaatcc cttcttccga ataaacggtg ttctcctgcc aactcctcgg
    65221 tcacaagtca caatatttat caaacatgag aagcaatttt tgtaaattcc ttcttatttc
    65281 ttgaagctga ccacttctgt cacaacctca gaagcggata aataatattg tttgatgata
    65341 ctttatcttt ttcccgtttg tttatagatc gaatgatatt atagaccaag aatcctacct
    65401 attaacatag ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag
    65461 ttaggattaa ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg
    65521 aactcgaacg ataatcgcaa ttaacgtttt gagaactgtg aagctttacg ggctttctta
    65581 agacctggtt ttttcttcgt atcattgaat aagtttgaaa aaccttgatt tataaaggtt
    65641 ttcccgtaaa tatattttat tatatttgat taaatttgaa atgtttgtgt tctaaaatgt
    65701 gttctaattt aaaggcagta tatttcgtat tttagattaa atttttaggc tcattcattc
    65761 tcaaatccat tctattcagt caataaaaat tttaaaaaga ctttataaac aaagatgttc
    65821 aagcgagcat cttatttttt tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa
    65881 caaaaaaaga aattatgtgt attaactatt ttgagtttgg ctgttcaagc aattgttgga
    65941 gcaattgctc ctacgattac ttttgctgat gaaattaccc atccacaaac ggtaaccgta
    66001 gaactagatt tagcacatca atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg
    66061 tcagaagtca ctgttccgca ttatgcagtg tacaacggct ttaaacaaga tattttctgt
    66121 attgaaccag gagtcccgat ttacaatgag ttcactcctg gctatgagaa aaacccgtta
    66181 ccagatatgt ccgataaagc caaattagtt tccgttttat ggaaaaaagt aggtacagat
    66241 gcagatactc atattgtggc acagaagatg atctggcaag aagttaatgg gtataccctt
    66301 cactcaataa aacgatcaga tggtagtgcc gtaaatatcg cagccattga ggcaaaaata
    66361 aatcaagcca ttgacgatta tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca
    66421 gtattagggc aatcgactac tgtgacggat acgaataatc tgaatttgtc agagttcgat
    66481 gaggttgtgg aaaatgccgc aaacattgat tatcgtgtga atggtaatca gttgataatc
    66541 accccaaatg ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc
    66601 ggaacacctg tcgcctataa aaaggctggc caacagactt taatggccgg ggcgattgat
    66661 aaacctaata cctacacaat caaaattgat gtggaaacgg agggttcttt aaagatcaaa
    66721 aaagtcgata aagaatcggg tgatgttgta ccaggaacgg ttttccattt agactttgga
    66781 aaaactttac ctgcaaaaga cgtgactact gacaaagaag gcattgctac attggatgag
    66841 attcctcatg gtacaaaagt aaccattacc gaaaaatcag taccggcgcc ttatacgatt
    66901 gataccaccc ccatgactac caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa
    66961 aacgcacggg aaaaagggca aatcattctg gataaaagcg gagttgaaac agggagtgat
    67021 ctttggaatg acaattattc cttagctggt aatacgtttg ccattcgcaa agacagtcca
    67081 accggtgaaa tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa
    67141 gagattgcta atgcgttgga attgggaacc tattatgtga ccgaaactaa agctagtcat
    67201 gggttcgtga atacgttcaa acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta
    67261 gcccctgtta ccagtaacgt aaaagggcaa aatcaagaag tcactggcga aaccactttg
    67321 acgaaagaag acaaagatac tggggataaa gcacaaggta aggcagtctt tgagggtgct
    67381 gaatacacct tgtttactgc taaggatgga aaagctgtta aatggagtga ggcttttaaa
    67441 ccagagttag cgaagggaac gaaaggttct gatgaaacgg tcactttgac tttagacgaa
    67501 aagaaccaag ttgccgttaa acatttagct atcaacgagt attattgcca agaaacaaaa
    67561 gcgcccgaag gatatagcct tgatgaaaca aagtatcctg tatccatcaa aaaggtggat
    67621 gatagcgaaa aaaatgccgt gattacccga aatgttacgg caaaagaaca agtgattcgc
    67681 tttggctttg atttctttaa atttgctgga tcagctgcgg gcactaccga aactggcttc
    67741 aatgatctga cctttaaagt gtcgccattg gaagggacca atgaaatcac aggtgccgaa
    67801 gatgaagcga ccacagctta taacgagcaa ttagggtttg atggttatgg caagtttgaa
    67861 aatcttcctt atggggatta tttacttgaa gaagtagaag ccccagaagg ttttcaaaag
    67921 attacgccat tagaaatccg ttctactttt aaggaaaaca aagaagactt tgtgaagagt
    67981 gagtatgtct ttaccattac cgaacaagac caaaaacaac caattaaaac ggtgaccgtt
    68041 ccttacgaga agctgacgaa caaagcattt tctgttagtt tgaaccgttt aatgctctat
    68101 gatttgcctg aggaagaaga tagtttaact tctcttgcga cttggaaaga cggaaacaaa
    68161 gaattaactt ctctggattc taccgagcta gttgataaat tgagttacaa tctacatgaa
    68221 atcaaagaag actggtatgt ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc
    68281 caagaaaaag atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc aacgttggcg
    68341 aacaaagaga aaacaggaac ttggaaaatt cagcataaat taaccgccga acaagtttta
    68401 aataaaacta ttgtcttatt caactatgtg tatgaaaata aggaagcttt tgaagcaggc
    68461 gataagccag tagcgaagga tgttagcttg aataaccaag cccaaactgt cagttgtaca
    68521 gtggaacacc atgtttctat ccaaacaaag gcccacctag aaaacggttc tcagaccttt
    68581 actcatggtg atgtggtgga tatgtttgat gatgtgtcga ttactcatga tgtactggat
    68641 ggctcaaaag aagctttcga aacaattctg tatgctttat taccggatgg tacaaacaaa
    68701 gaaatttgga aatctggcaa gattgattat gaagtaaatg ataaagaatt caccaaaact
    68761 gtgcttgcga aaaaagtaga taccggaaaa tatccagaag gaaccaagtt tacttttgcg
    68821 gaaatcaatt acgataaaga tgggaatatc aatggaaaac acaatgaaga tttgaaagaa
    68881 aaatctcaaa ccttaacacc aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg
    68941 gaaacaccaa ctgttccaag tggctctcaa gaatctagtc ccacagtgaa gaccttccct
    69001 caaaccgggg agaaaaattc caatgtttta ctgttcattg gttttacttt gatttttgcg
    69061 acggcgggct attatttctg gaatcgccgg aactaaggtg atgagatgat acaagtaaat
    69121 cctaagaacc aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt tttgtatcca
    69181 aaactaacaa tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc
    69241 tgaaaccttt ggaaagttaa gttatgccgg tgaaggagaa attataacag aaggatatgg
    69301 acggaatact acggtgattg gtcgcagtta tcatttgtat tccagtaagc aacgggccga
    69361 tgatattgag gtggtagtag ctgcggaagc cggtgaaaag gattttgatc aagaccaacc
    69421 gctaaaagcc gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt
    69481 tactgattac gtgttgtatg ttgatgattt agtgaaagca taggaggaaa agaagatgag
    69541 attaacagaa ggtattgtcg tagattcagg tttaacattt gggaagttgc gtttttccgc
    69601 attacgccga gaagtacgga agcaaaatga ggatggaaca attggtaatg aggtaaaaga
    69661 acgaacctat aatttaaaat cctctgcaca agggcgaatg attcaagtta gtatcccagc
    69721 taatgtaccc ttacgcgagt ttgcttatga tgcagaggta gagttggtca atccaatcgt
    69781 ggataccgtt gccaattatg tttttcgaga aggaactacc gttaattggt tcattaaggc
    69841 ggatgattta gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag
    69901 aaagaaatag gtgaagatta tggaaattaa tgttgaacat attttagatt gtctcgatca
    69961 atatggaaaa ggcgagctga ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa
    70021 aatgtttctg attatacatc aaggtttact tggagaaaat agcgacagtg aagaagactc
    70081 tgatgtacta gactggttag gagaagaaga gtctttcttt atagttgtcg aagtaaatga
    70141 aaacttatgt cggcaggcag aatccatttt ggaagaaatt ggtgtggaga tgccggatgc
    70201 tattgaaggc tttttaaatc aattggttga aacgaaacaa ctcccctcct cgtagcagta
    70261 aatgactagc ataacttttt gaaattgcct aatgaaatcc tattgggctt tttcgattgg
    70321 aggtggaaac catcaaaatg tacaaaggac atcgcatacg cgcaggagat cagcacttgg
    70381 tttatcactt tgttcttggc tggctactcg ctctgttcat cggttggatg agcgtctttt
    70441 attttcaaga gtttagacaa tttgatattt ctaagctatc actttctact attgaaatcg
    70501 tttggtctat aaaagattta gtatgtctgc ttggaagtct cgctttttca ggagctatga
    70561 tattgcttta tattcacttt tttcttgatc attggcgaag cttgtggcat cgacaaaagc
    70621 tagctcggat gattctagag aaccattggt atgaagtgaa acaaactcaa agtgaaggct
    70681 ttttcaaaga tttgaatagt agccgaacca gagagactat cagttacttc cccaaaatct
    70741 gttatcgaat gaaagatgga ttactgtcta ttcgcgttca aatttcactt gggaaatacc
    70801 aagatcagct tttaaaattg gaaaagaagt tagaaagcgg actgtattgt gagttagtag
    70861 aaaaggaact caaagactcc tatgtggagt atactttact gtatgatatg atcgccaatc
    70921 gaattgggat agacgaagta gtggcagaaa atgggactct gcgattgatg aaaaatcaag
    70981 tatgggctta tgattcttta ccccatatgt taatcgctgg tggcacgggt ggagggaaga
    71041 cctatttcct cctcaccatt atcgaagcac tattgaagtc agatgcagaa ttgtttatcc
    71101 tcgatcccaa aaatgcagat ttagctgatt taggtacggt gatgcctcat gtttattcac
    71161 aaaaggaaga aatttctgct tgtgtggaag atttctatga acgtatgatg gctcgtagca
    71221 aggcaatgaa agaaatgtct aactacaaaa caggagagaa ttatgcctat cttggacttc
    71281 cacccaactt tttaatcttt gatgaatacg tggcttatat ggagatgtta acgacgaaag
    71341 aaagtgcggt gattttgaat aagctcaaac aaatcgtgat gttgggtcgt caatctggtt
    71401 tctttctgat tttggcttgc caaaggccgg atgcgaaata tttaggagac ggaatccgcg
    71461 atcaattcaa ctttcgcgtg gctttgggtc gtatgagtga actcggatat tccatgatgt
    71521 ttggggaggt cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt tatgtggata
    71581 caggtggcag tgtgattagt gagttttata cgccacttgt accaaaaggg tatgatttct
    71641 tagattcaat tagtaaatta gaaaagatga atgattgagt gatcttcaat actgacttta
    71701 tacttcaagt aacgtataat acttttatat agtaggaaga aaggggataa ttaatatgga
    71761 aagtaatgat tcttatttag agtatgcaaa agcaaataag gataagttta tttcttatat
    71821 tatcaaaggg aaatcccccg aagttggacg agacgcagtt tttatggcag ggagtcctgg
    71881 ggctggaaaa tcagaggttg ccattggatt agctgagaat tatgataacc atgtgattat
    71941 tgatgcggat tactttcgca cccagtttcc tgattataat ggaaaaaact ctagtctctt
    72001 tcaaaaagca tcttcttggt tggtagagca ggcatttaga tatgtcttag atcatggcta
    72061 ttcttttatt ctggatgcaa cttttgcaat attaagtgct gaaaaaaata tctcgcgggc
    72121 gttgaaaaaa cattttcgag tgacgatttt ctatgtttat caagacccta aaatcgcttg
    72181 gaaatttaca caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa aggcatttat
    72241 taacgctttt ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac atccagatgt
    72301 attgcttcat atcataataa aagattatac gaataatatt tccgaagttc attatgctac
    72361 ggataatcta gaattatttt taccactagg gtatagtagg aaagatttgg aggttcaatt
    72421 acatgactga gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg
    72481 atttatcgga aaatgctact gcaaaagagt tcaaaacggt gattaaatat gtagccgatc
    72541 aggccaatga aaaacaacgg aaattagttg gattaaataa aaaatagtag gacaaattaa
    72601 taatgtatga gccaagagca aaatgttctt ggcttttttc tttgcaatga atgaggtaat
    72661 gtatgagatt tttagattta ttcgccggta ttggtggctt tcgtttaggt atggaacagg
    72721 cgagccatca ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt agttataaag
    72781 caattcatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagatgagt
    72841 ttattcaatc cctcggacca gtggacatcc tttgcggcgg atttccgtgc caagcttttt
    72901 caattgcggg aaagcggcaa agattttccg atactcgagg tactttattc tttgaaatcg
    72961 ctcggttcac cactcttctc cgacctaagc ttttattcct tgagaacgtc cgaggattgc
    73021 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
    73081 atgacgtgga gtggtaggtg cttaactcaa aggcctacat tccccaaagc cgggagcgga
    73141 tattccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggac
    73201 cgtcttcaac atctgatcaa aacatcagaa atttggtaaa tatcaatcct tccaatcgag
    73261 gaatgggtgg acaagtctat ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag
    73321 gaatcaaaat cgcactgcca gcaagggatg gaatctctgt agcaggacta ttaccgggaa
    73381 agtttgaaca aggtaatcga gtttatgaag ttactggaac cgcccccacc ttgtcaacga
    73441 aacaaggtgg tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc
    73501 cagaagacag tgtgaatctg gcttttccta attccacaag tcgccgaggt cgtctgggaa
    73561 aacagagtgt tcatacctta ctaacaggag accaacaagc gatcgtcacg gatcagtatc
    73621 aaattcggaa gctaacacct agagagtgtt ggcgacttca gggctttccc gattgggctt
    73681 ttgatcgaga ttctcaagtc aattcagata gtcagttgta taagcaagcc ggaaactctg
    73741 taacagttcc ggtcattttt gatattgcca gaagattaaa agagttggat gtacatggtt
    73801 tatgatgagt tgattggaga aatttattgg gtgattggaa agattcagtc agacccagaa
    73861 ttagaagaac aattgcgacg gttaaaattt gatattcgca agaatggtgt caaagtcgct
    73921 ggcgatccat atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt
    73981 gcagaatttg agcggattgc ggaccaagca aaagaaccag atattcgtca atacttgtta
    74041 gaaatgaaag cagagttgga agtaaatggt attacagatg agtaacctaa accaacacaa
    74101 ctgactgtta ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg
    74161 cctgttcatg gacggcgtta gccgaccatg atgtctcacc cccgtatcta acaggggggt
    74221 acaaaaacac aaaaaataag taagcaacca ttaagaatgc ttgttgtatc aaagatagta
    74281 agtaatttgt ataggtgtta actcatttga aaaagttaac accttgaatt tggaggtgag
    74341 aacttggcac aacggaattt agattatcgg ctattaaaag accgacgcaa cgaatacggc
    74401 gtttcacaaa ataaattggc tacggcttgt ggacttagtc ggccgtactt aaatcagata
    74461 gaaaatggtg gcgtaaccgc atctataaaa accatgagga aaatttttga ccaactggaa
    74521 agctttaatc ccgatttgcc tttgacgtta ctgtttgact atgtaaggat tcgctttccc
    74581 acaacggatg cacgaaaaat tattcaagaa attctccatt tgaaatttga gtatatgctc
    74641 catgaagatt atgcctttta ctcctatcaa gaacagtatg tcatgggtga tattgtggtg
    74701 atgttgtctc atgaagaaga taaaggtgtg cttttagaat taaaggggcg aggttgccga
    74761 caatttgaaa cttttttact cgctcaaaag cgtagctggt acgacttttt cgaggattgt
    74821 ctaaaggccg gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg agtggggcta
    74881 ttggatatac ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt
    74941 acctttaaaa gttatcgttc cggggaactt ttaaaggctg atgaaaagga cggcatgggc
    75001 aataccttat atatcggcag tctcaagagt gaggtttatt tctgtttgta tgaaaaagac
    75061 tatgaacaat atatcaagtt agggattcca ttagaccaaa ctgagacgaa aaatcgtttt
    75121 gagattcgac tgaaaaatga ccgtgctttt catgcgattc aagatttgtt aaaaggtcgt
    75181 agcattgaga gcaccacgtt ttccattatc aatcgttact tgcgatttgc ggataaagta
    75241 gaaggtaaaa ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt tattggacgg
    75301 aatcggaaag agattcaatt aacttccgag ccaaaaccgt ataccattga acgaaccttg
    75361 aattggctag gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg
    75421 aatcggacaa cctatatcca ggatatggta cagaatgcac gcttatcaga ccgacataaa
    75481 aagattttgg agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg
    75541 tcatgggaat tgtaaaagat atttttttgg tatttggtgg cagtatgctt ggtgttactg
    75601 tgatgtgctt gatgaatgca agtgcagcag cggatcgagc aatggaaaag aaaggaaagt
    75661 gacaagatga atttcggaca aaatttatgt gcgactcgtt ccttagtgaa aagctaaggc
    75721 actaataaaa gaacgaaaaa gtttggttat taggagaact gataatctga caggtggaat
    75781 gatgaagtaa cgctctgaaa cgcctgccct gagcctccga caagcattgt atgaggaaac
    75841 tcagaatgtt tgaagctcgg taaagacggc tgaaagatac cgtaatagtt gatgaacata
    75901 tatcttctat cgaaaactat ccagaggtgg gtagcgtatc caacaggtcg ggggtctata
    75961 aaatatctat ggttagaatg ttctataagg aactgacgaa acagcgaatg tacgggtcta
    76021 aatttgaact ttgtagaaat gcaaaggtac gagaaatcgt agctggtgtg gcaaagtaag
    76081 aatataggtt atgaaatgcc atataacgtt acaggcggta tcaagccagc aggcttatag
    76141 ctagtgacct aaggatatat atgtatagat aagattatcg gaacgaggaa aggtattggg
    76201 ttctcatcaa gagaataaac tgacgaagaa caataagcag ttgaactaat gctgaaaagc
    76261 agagggcgaa cctaagaaat tcttgtaatg agaatggagg aatgccctca agtcgtgtat
    76321 atattagaaa agtattatgt tgtttcaata aggattacga gtaagataaa aaagctcact
    76381 caatttcaag taaaggagtg aagccttatg cctaaaaata aaaatgaaac attgtgtgta
    76441 gaagatttaa gacacgctga atattacgaa atgcaaaaca cctttgatga tttatatgcc
    76501 aaaagtaaga atggagatat tttcactcat cttatggata ttatcctatc gagagaaaat
    76561 atattactgg catatcgaaa tatcaaagca aatgcaggca gtaaaacagc aggaacagac
    76621 ggtacaataa ttaaagatat tggcaaattg ccagcagaaa cagttgtcaa aaaagtaaga
    76681 tacattgttg caggcagtcc acatgggtat cgacctaagc cagtgaggcg aaaagaaata
    76741 ccaaaaccaa atggtaaaac cagacctcta ggtattcctt gtatgtggga caggcttata
    76801 caacaatgta taaaacaagt attagaacca atctgcgaag ctaaatttag tgaaaacagc
    76861 tatggtttta gacctaatag gtcagttgaa aatgcaataa aagcaaccta taaccgccta
    76921 caaatcagcc agctacatta tgtgattgaa tttgatatta agggtttctt tgacaacgtg
    76981 aaccattcca agctgataaa acagatatgg gctatgggaa tacgggacaa gcacttgata
    77041 ttcattttaa aaaggatatt aaaagctcca atcaaaatga ctaacggtac aataacatat
    77101 ccagagaaag gaactccgca agggggaata atatccccac tactagcaaa cattgttctt
    77161 aatgagttag accattgggt agaaagccaa tggcaggaaa atcctgttac aaaaaactat
    77221 gtggttcata tcaataaaag cggtagccca tgtaaaagca acgcttacaa agaaatgaag
    77281 aaaacaaagt taaaggaaat gtatatggtt cgttatgcag atgattttag agttttctgc
    77341 cgctacaagg aaagtgctga aaaagccaaa atagcaatca ctcaatggat tgaacaacga
    77401 ttaaaattgg aagtatcaca agaaaaaaca agaattgtaa atgttagaaa aagatactcc
    77461 gactttcttg gtttcaagat taaaatgata ccgagaagaa agaagttagt tgttaaatca
    77521 catataagtg ataaacaatt caaaaaccaa aaagacaagc tggtatcaca ggcaaagaaa
    77581 attgcttctc cctcaaaagg gaaaacggaa ttagaagaaa caagacttta taattcaatg
    77641 gtattaggta tgcaaaatta ttatcagatt gcaacgaatg taaatctgga ttgtatgaaa
    77701 ctgaatagag ctgtaatggc tatcttcact aacagattag gtacaaacaa aaggggcaga
    77761 ttgagaaaaa caggcagaca acttacgaca aaggaggcaa caagatatgg taaatctaaa
    77821 atgcttcggt atgttgctgg cgagggcgaa cctattttcc ctgttggata tatccaacac
    77881 agaaaaccaa tggcaaaggt gtatttagcg aattgctaca ctctagaggg aagaaaattt
    77941 atccacacca atctaagtgt tgataaatta ctgatgtacc agttaatgaa attatctttg
    78001 gaaaatcgca caatagaata cgcagacaat cgaatttcgc tattttctgc tcaacatgga
    78061 aaatgtgcta taactctgga agaatttcaa caagctaatg aaatacattg tcatcatatt
    78121 gtaccaactg gagctggtgg aaatgacgat tataaaaatc ttattttggt aaaagaggcg
    78181 gtacaccgtc taatacacgc taaaacagaa gaaacaatac aaaaatatat agctctattg
    78241 aagctaaata aagtccagct cataagacta aataaatata gagtattagc tggcaatcaa
    78301 gaattaaact tgatataggt ctactacata aatacttttc aatataaaca cgatggaacg
    78361 cggagtgcgg tgaaagtcgc acgctccgtg tgaatggggg gaaaagtttg agatatttat
    78421 aatatcagga acttacctat ccatatatca atggttttta accaatgccc aatccttagt
    78481 cttactggca attgtggtca ttggtttgtt tttaggattt aagcgggagt tttccaaact
    78541 gattggcttt ttggtgattg ctttgattgc ggtcggattg gtctttaatg cttctggggt
    78601 gaaggatgtc ttactcagtt tgtttaaccg aattgtcggt gcgtaagggg gaagcacgat
    78661 ggaacagatg cgtgtttata ttgcgaacct cgggaaatat aacgaaggtg aattggtggg
    78721 ggcgtggttt accccacctg tcgattttga tgaagtgaaa gaacgaattg ggttaaatga
    78781 cgaatacgag gaatatgcaa ttcatgatta tgaacttccc tttgagattg atgagtatac
    78841 gccaattgag gaaattaatc ggttgtgtgg cttagcggag gaacttgaag gaacgccagt
    78901 tggtgaagtt gcttctgaga ttcaacatgc tttcttcaat tcttttgagg agatggtaga
    78961 gcatgtggat gatattatct attatccaga ttgcgatgat atgagtgatg tagcatattt
    79021 cttgattgta gaagctggtg attttggaga agtaccagaa cacttgaaga cgtatattga
    79081 ttttgatgcg tgtggtagag acttggagat tggaggcaat tatttggtaa cgagtcgagg
    79141 tgtttttgag tatccatatt aacaaaatta ttgaagggta gttgatttct aaactatcct
    79201 tctttttaaa ggaatgattt gatgaagaaa ataaaaagct acaccagtat ttgggcagtt
    79261 gaaaaggtca tttatgcaat taatgacttt caactgcctt ttcctgtgac ttttaaccag
    79321 atggcttggt ttgttttatc cttgctgttt gtcatcgtat ttgcccatgt gcctccacta
    79381 tctatgatag agggggcctt tttaaaatac ttaggtatac cggtggcagt tacttggttc
    79441 atgtcgcaaa aaacgtttga cggtaagaaa ccattgggat ttttacgatc ttttattagc
    79501 tatcacttac gatttaaggt cactttcgca ggaaagaaag tcaaagaaca gaagaaacga
    79561 tgggacgaac ccattactct ggtaaggagt gtgaactatg tacccgatta agtatattga
    79621 aaataatctt gtctttaatc aagagggaga atgttttgcc tactatgagt taatacctta
    79681 caattactcc tttttgtcac ctgaacagaa atatcaagtc cacgataact ttcgtcaatt
    79741 gattgcgcaa aatcgtgaag gcaagattca tgccttacaa attgccacag aaagtagtat
    79801 tcgtgccacg caagaacgat caaaaaagga aattaccggg cgattaacag aagtagcgaa
    79861 acaacgaatt gatttacaga cagaggcatt agtatccatg attggcgata gtcagattga
    79921 ttatcgcttt tttattggct tcaaactgat tgcgacagat gaagaggtca atctaaagaa
    79981 tctcaagaaa tctttcttct ccggctttca agaatttgtc tatggagtga atcatcattt
    80041 gatgggcgat tttgtttcct tatccaatga agaaattcgc cgatatacca agcttgaaaa
    80101 actaatggaa agtaaattgg ctcgaagatt taaagtcaga cgagtcacac cgagtgatct
    80161 gatgtatttg attgaacata tctatggcga aaagggtaca ccatttgaag aatatgaatt
    80221 tcagctacca aagaaaaagc tgaagtcgga aactttggtg aaacgttacg acttattacg
    80281 tcccagtcgt tgtttgattg aagaaaagcc ccggtactta tgcatggaac atgagaatca
    80341 tgaatcatat gtagcgtact taacaattaa tacaattgtg ggggaaatgg agtttccctc
    80401 ctcagaactt ttctattacc agcaacaaca atttacattc ccaattgaca ctagtatgaa
    80461 tgtggagatt gtgacgaaca aaaaggcact tgctaccgtt cgcaataaga aaaaggaatt
    80521 aaaagacttg gacaaccatg cctatcaatc tgacaatgaa acaaattcca atgtcttaga
    80581 tgcattggat tccgtagatg aattggaaac taccttggat cagtccaaag aatccatgta
    80641 caaactgagc tatgtggttc gagtaagtgc cgaatcagta gatgaactaa agcgccggtg
    80701 tgatgaagtg ctagattttt atgatgatac caatgtaaaa ctagtccggc cttttggcga
    80761 tatgatgggg ctacatgaag aattcttacc ttctagtaag cgatacatga atgattatat
    80821 ccagtatgtt acgtctgatt ttctcgccgg acttggtttt ggcgccactc agatgttagg
    80881 ggagttggaa ggcatttact ttggttataa cgtagatacg gggcgcaatg tttacctgaa
    80941 accggcattg gcctcacaag gggtcaagga ttcagttacc aatgcgctag cagcggcatt
    81001 cctcggttca ctcggtggcg gaaagtcctt tagtaacaat ctcttagttt attacgccgt
    81061 cctttttggt ggacaagctg tgattgtcga tccaaaaggg gaacggggtg gctggaaaga
    81121 aaccttgcca gaaattgcgg atgaaatcaa tattctcaac ttaactagta agccaaaaaa
    81181 tcaaggatta cttgatcctt atgtgattat ggagaaaaag aaggattctg aaagtctagc
    81241 aattgatatt ctgacctttt taacaggaat ttctagtcga gatggggaga agtttccagt
    81301 gttgcgtaag gccattcgga cggttaccca aagcaatcaa cggggattat tacaagtaat
    81361 taatgagctt cgaaaagaag gctcaccagt tgcggaaaat atcgcggatc acatcgagtc
    81421 ctttaccgat tatgattttg cccatttgct tttctcagat ggttcgatta cccagtcgat
    81481 tagtttagat aggcagttaa atatcattca agtagcggat ttggttttac cagatgcaga
    81541 aaccactttt gaagaatata ccaccatgga attgcttagt gtttccatgc taatcgtcat
    81601 ttccactttt gccttggact ttatccatag taatcgagaa atcttcaaaa ttgtagactt
    81661 agatgaggcg tggagttttc ttcaagtagc gcaagggaaa acattatcga ataagctcgt
    81721 tcgtgctggg cgggctatga atgccggggt ttactttgtc acgcaaaata gtgatgattt
    81781 actggatgaa cgcttgaaga ataatatcgg gttgaagttt gcgtttcgca gtaccgatat
    81841 tcatgaaatc aagaaaacct tggaattctt tggcttggat cgagaagatg aaagcaatca
    81901 aaaacgccta cgagatttgg aaaatggcca atgcttgatg caggatttgt atggtcgggt
    81961 aggcgtggtt caggtacatc cagtctttga agaactcttc catgcctttg atacccgtcc
    82021 acctgttcaa gagagaatgg agtgagggga tgaaaaagaa actttttcgg attgtgggaa
    82081 tcgtttttat tagcatactc actctgatag ttctattgag tttagttggt acggtagccg
    82141 aggcgacagg gctagttgat gatacagttg aagcaggaaa tctatattcc aaatattcac
    82201 tgaacaacta tcaactagat ttctttgtgg atagctcttg ggattggttg ccttggaatt
    82261 ggggtgatgg cttagggaaa agcgtgatgt atggtcttta tgccattact aatttcattt
    82321 ggacggtgag tttgtatttg tcgaacgcca cgggttatgt ggttcaagag gcttacaaat
    82381 tagatttcat ttcggatacg gctgaaagta ttggaaagaa tatccaaaca ttggctggta
    82441 ttactgaaaa tggactccag acctccggat tttattttgg gtttctatta ttgatgattt
    82501 tagctctggg ggtgtatgtg gcgtataccg gattattaaa acgagaaacc acgaaagcag
    82561 ttcgagccgt gattaatttt gtgatgattt ttctactatc gggttcgttc attgcttatg
    82621 cgcctaccta tatcacaaaa atcaatgact ttagctcaga tgttagtgaa gcggccctta
    82681 cccttggaac cgagatcgtt gtacccaatt cagaaagcca aggaaaagat agtgtggatt
    82741 tgattcggga tagtttgttt tcgattcaag tccaacaacc ttggttacta ctgcaatttg
    82801 atgattccaa catagaagaa attggtgaag accgggtcaa caagattcta tctgtgagtc
    82861 cagatgaaaa caaaggcaaa gatcgggaag aagcggtcaa ggcagagatt gaagacaatg
    82921 acaatgcgaa tctcagtatt accaagacca tgaaccgttt agggaccgtg gtctttttgg
    82981 tattgttcaa tattggcatt tccttctttg tttttcttct aacagggatt atgttgttct
    83041 cgcaaattct cttcattatc tttgcgatgt ttctgccaat tagtttctta ttgtccatgt
    83101 tgccgactta tgaaagttta ggaaagaagg cgattattcg tttattcaat acgattatga
    83161 tgcgagcggg tgttactttg gtgattacga ctgcatttag catctccacg atgtttttca
    83221 atatttcggc aacctatcca tttttcatgg ttgcttttct gcaaattgtg acctttgccg
    83281 gtatctattt caaattgggc gacattatga gtatgttcaa cctccagagt aatgatagcc
    83341 agtcaatggg aagacgcgtc atgcgaaaac cgcaaatgtt aatgaatcga aaattgcggc
    83401 aactcaatcg aaatgtggga cgagctttgg cttttggtgg tggtgcagca gtcggaaata
    83461 agttggcaaa ggaacaatca aaatccaaat ttaagccatc cggttcttct ctacgaaaga
    83521 attcacgatt accgaacgat cacgaaatac tatctgactt aactaaggag aatccagttt
    83581 caaataataa gaaacaatcc cgaatgaatt tggctgggag aaaaatcggc aaagttttgg
    83641 atacccaagc tttggtgaaa gataagacta aacaagtaaa ggatcaggta aggaatacgc
    83701 caacgaattt gaaatacaat ctccataaag gaatcgaaaa gtcgaaaaaa gcaccagaag
    83761 aattcaagcg cggactgttc caagaaaaag cggatcgagt ggatttgcga gaaaaacaac
    83821 gacaacgaag aaatgcgaaa atggacgaaa aacgcaagat actgagtgaa gcaacaaatc
    83881 gtcagggaaa aagaagggcg aatattccaa tagataaagc agctcaacct cagaaagata
    83941 tgatgctgaa aaaagaagca ccaaagcgga ttgtgagagc aaatctgaat cccgaaatca
    84001 aacgaaaact aactagtcaa gaaatcattt caaaagggaa aagccaatca gcaagtaaga
    84061 aaaattcttt ccaacaagtg aaacagaggt ccactctgcc aaagcagacc aatcaaaaag
    84121 tacaaaaaag aatgaaccat cgtccaaaat caaggtcagg tgagaaaaaa tgaacttgaa
    84181 gaaagtgggt gttatttcat taacttttct gaccatctct tttatgggta ttttgatgtg
    84241 tgtgggtttg ttgtttggtg aagacagtga gggaaatggt agttctggca cttctcctgg
    84301 cggaactagt gtctcagaag aagtattgaa acaccgtgca atggtggaaa agtatgccaa
    84361 agaatcggat ataacagagt atattccaat tcttttggcg attattcaag tggaatctgg
    84421 tggcacaatg gaagacgtta tgcaaagttc tgagtcagcc ggattaccac ctaattcatt
    84481 aagtaccgag gcatccatca aacaaggctg tctctatttt gcgtctttgg ttaagcgttc
    84541 caaagaacta gggtgtgatc aagatagtat tattcaggcc tataactatg gtggcgggta
    84601 tctagatcat gtcgggaaaa acgggaagaa gcatagtttt gctttagctg aatctttttc
    84661 aaaggaaaaa tcaggtggtc aaaaagtcga ttaccccaac ccaatcgcga ttaaggaaaa
    84721 tggtgggtgg cggtataatt atggcaatat gttttactgt ttattagtga agcaatacct
    84781 aactacaact cagtttgatg acaaaatagt ccaagaaatt tttgatgagg catttaagta
    84841 tgaaggaacg gcctatgtgt ttggtggctc aagtccagaa acggggtttg attgtagtgg
    84901 cttgacccaa tggtgttatg ccaaagttga cctgaaactt cctcgggtgg ctcaggatca
    84961 atatgatgcc atgactcata ttgatctaaa agatgccaaa ccaggtgatt tagttttctt
    85021 tcattcaacc tatgatgccg gtacttatgt gacccatgtg ggcatttacg caggggagaa
    85081 tcgcatgttt catgcaggag atccagtggg gtggacgaat ctaacggaaa actattggcg
    85141 gcagcatctt atcggagctg gccgatacaa ataagagaaa agaggaaaaa gaatgaagat
    85201 aaaaattgaa cggaatcaaa aggaaaagag cccaaaaaag aaaaaagaac gagttgtatc
    85261 cgtgggtaag catcgaaaaa tggtgcttgc cctttggctt ttattgtctt gtagtcttgc
    85321 ttttggcgtt tataaaaatt tcaccgccat tgatcaacat acaactcatg aaaaagtggt
    85381 tataaaagag aaggtagtga atacttctgg cgttgaaagt tttacagagg attttgcgaa
    85441 agaatatttt tcatggaaaa ataacaaaga agcaattgaa aagagaatga gtaatctcga
    85501 acagtacctt acagaaaacg gtctcgcttt aatccaagat atggttcgag cagatattcc
    85561 cacgagttcc gaagttcaat ctgtaaaaat tctagatgtt gaaaaacact cggaagaatt
    85621 tgtcgtttca tttttagtgg agcaacatat tacagaggga aaaaagacgc aatcaatttc
    85681 ttctgcatat cgagtgacaa tctttgaaga tgaaaacagg aatcatttag taactagttt
    85741 gccaacaatg attggaaagc cagacaaggc aaaatatgaa gcaaaacaag tagaaaacga
    85801 ttcagaaatt gatgccaaaa caacggaaga aatcaccgat tttttagaaa cattttttaa
    85861 actttatcca tcagcttctg aaaaagaatt ggagtattat gttgaaaatg gtgcgatgcg
    85921 accaatcaat attgacttaa agttttcgga gttagagaat ccagtttatc tacaaaacgg
    85981 aaaaaatttt ctagtgagta caaagataaa ataccttgat aatgtatcaa aaaccactag
    86041 ctactttcag tacaaattag tattacaaaa aaatggaaac tggaaaatcg ttgatgttaa
    86101 ataataatga ctcaatctaa ttttattaga agttataagt agtgtaactt atttcaggca
    86161 gaagtcgccc atcctctata ggtgggtgat gaatgccatt cggtaactcg aaaaccgaca
    86221 attaagacat agtgcctttt tattttaatc catgtcataa tcagtctata taggaaatgg
    86281 ttgatatacc atggaattag atacaaataa tcattcagtt tttcttcttg gttaccccct
    86341 tattttagta gtgaaacatt gtaaacatgt cattgatgat gtaatgtcag cttatgcaaa
    86401 aactgccttt gaaagaattt cagaatcaca tcacattacc ttagatgaat gaaatcgtga
    86461 taaagaatac tttcatatta tgctcaaagc acaacctaaa accgaattaa caaaattcag
    86521 caatgcctaa gctcacgtct gataaaatga gactttctaa gggtcaaaaa tttcctttga
    86581 atacgattat tgcttagata aaagtaaaag ccttagaact acggggatag cttggttaat
    86641 aagagatatc gttgttagta aagacatagg cttgcaagta tgctctattc ccaagaatct
    86701 cccccttcaa gcaccaagga tcgttaaatt cagctaagtg gcaagtaatt cactagacga
    86761 taattatctc catatttcaa tctcagcttt tccatttttc atagcaaatt ccgttatttt
    86821 tatagcatct aaaagattca gcttttccac tgatctttca ccacttttca gcttttttat
    86881 tgtgcttaaa ttgataccag taccttttga tattgaattg gaagtaaaat cattaagggt
    86941 tttttttatt aaattcatat ccgcaattcc agcgttcata agaatgaaga ttctcctctc
    87001 atagactact ttgaaaatgg tactacagtg gctgtttata tatttcagta ggttactgta
    87061 gtggtcatct tattataatt agaaaaggaa cctaaatcaa aaatgctaaa gaaaattttc
    87121 atagtattaa gcctctcatg actaaagcca cggaattctt gataaaagag tatacttgca
    87181 agcctaaaca gcggtatctc ttattaacca agctatcccc gttgttccta aggttcttga
    87241 tgttttctaa ggcaatgctt gtattcatag cccttggtca aaatattgat actatcgttg
    87301 acgtttcgat tatgatgtat acggcaaata ggacaagtcc attctcgaat tgtgagtgac
    87361 ttcttaccat ctgtgtgtct acattctgaa ccaatttgac tagacggaaa tcatttatct
    87421 atcttaatga ttttttgaca gaagtatcga aaatctttac ataatctggg tgatgtattc
    87481 ttatgcagat gagagcgttg gctccgtatt cataaccagt agcctctaaa acgtagtcca
    87541 tcggtgggat acctagcttg cggttgccag cggctttcgc agtcttcttg tgatcaaatc
    87601 aagcatctca ttgagcacta gtattttgaa gataatactt gagtttttcg gcttttagaa
    87661 gttcacttca tcttccatca tccatggaaa tcccactcac ccatacgcag gcctccaaaa
    87721 attcttcaac agtggttttt tcagacattt ttctgaatac ctccttgttt tttgatacgc
    87781 cagatagtca ggaaaaaggc aacaaaacac aaaacaaagc tgcctaaaaa ggtaatcttc
    87841 attccatata aaaagacatc cggccgattt gcgatatagt tagttacttg ctggcccatc
    87901 ttagcagaca tcgcttgata taagaccgtc gtagaaagcg aaatcccgct gaccatcccc
    87961 aaatttcggg caaaggaatt catcgagcct gccagtccca agttttcgat agtgaccgag
    88021 ctcataacca tcgtattatt gggggactgg aacaaggcat tgccggcacc catgatggca
    88081 gtagcgacaa tgtatagcca caaaggagag tccactgaca aaaacatata catgacctgg
    88141 gttaccgaca ataagcccaa gcctactaat accaaaatct ccgggccaac ccggtcggtc
    88201 aggtagccgc ttaaaggcgc cccgacgacc atcatcaaag gaaagaccat catcaacagc
    88261 ccagcatagc taggactcaa cccgcgagca ttttgcagat aaaaaggaat cactacattg
    88321 acaaagaagt tggaagaaaa aatcaacaag gctgtaatca agctcatggt aaaaatcttg
    88381 ttcttaaaaa taccgaaaga aatcaatgga gaggctgctt tcttttcaac ttttaaaaag
    88441 ctgacaaagg caatcactga aattacgaac agtacaaggg ggactggatg gaaaaagcca
    88501 ctttcttgcc ccaaaaagat tgctccaaaa aagctgataa tcgtagccgc aatggcagca
    88561 aagccccctc catcgattgt atggccgctc atggtgatat cttttggtaa gaatttttca
    88621 gcaatcaaca tcgttacgat tcccaccggt acattgatcc aaaaaatgta cgcccaagaa
    88681 aattgggaca aaatcaagcc gccaagacct ggtccagcaa tcgagcctaa tgaaacaaac
    88741 gcacctattg ctcccaatgc ccgaccacgt tcatgtatgg gaaagacttc cgtgatgatt
    88801 ccggcatttg tagccatagt catactagca ccaatggctt gtaccacccg tgcgaacaat
    88861 aaaataccta gcgaatgatt gaacccacac aacagcgatc ccaagacaaa gatcagcgta
    88921 ccaatccgaa atactttgat tttcccacgg ctatcgccaa ttttaccaaa cagcaacaaa
    88981 caagaacaga caatcatcag ataaacggac actacccatt ccgcttgatt cattggtatg
    89041 cccatatcat ctgagatggt aggtagtgca atattgacaa tactgccatc caatgtggac
    89101 atgaaggtaa acatagatat agcaatcaaa atccaccagc gatttttttg gacttttgca
    89161 tcttcttgat aactatacat aataggattt cctttctaat gatcgtgtag ctaagaacaa
    89221 tataacctat gtctactgcc caacaacttc tcgcgactct agccatagga agcttgagta
    89281 atttcgattt gatatcctca tgtaaccatg taagttggag atgctttatt tgaacttact
    89341 ctatagtaac tagtgataat tcgctttact ttcttctagc aatttctaaa agtcgacttc
    89401 attgttcttc agattaatag tgtgagctat ctcgtgagac gtaagcaaat ttccattcta
    89461 ctttttccca attttttcat atttatggtg ttgcacctat ttgttttcca atttgctttt
    89521 caaatacatt gctatgata
//
LOCUS       DAFGZE010000002        80331 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.002, whole genome shotgun sequence.
ACCESSION   DAFGZE010000002 DAFGZE010000000
VERSION     DAFGZE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 80331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.002"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            282..653
                     /locus_tag="LTU54_000086"
     CDS             282..653
                     /locus_tag="LTU54_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HBL8294829.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(927..1619)
                     /locus_tag="LTU54_000087"
     CDS             complement(927..1619)
                     /locus_tag="LTU54_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8294830.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(1622..2407)
                     /locus_tag="LTU54_000088"
     CDS             complement(1622..2407)
                     /locus_tag="LTU54_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8294831.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            2644..4044
                     /gene="lacG"
                     /locus_tag="LTU54_000089"
     CDS             2644..4044
                     /gene="lacG"
                     /locus_tag="LTU54_000089"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL8294832.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            4069..4386
                     /locus_tag="LTU54_000090"
     CDS             4069..4386
                     /locus_tag="LTU54_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL8294833.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            4399..6072
                     /locus_tag="LTU54_000091"
     CDS             4399..6072
                     /locus_tag="LTU54_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL8294834.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            6086..7717
                     /locus_tag="LTU54_000092"
     CDS             6086..7717
                     /locus_tag="LTU54_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HBL8294835.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(8208..9773)
                     /gene="guaA"
                     /locus_tag="LTU54_000093"
     CDS             complement(8208..9773)
                     /gene="guaA"
                     /locus_tag="LTU54_000093"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBL8294836.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(9832..10755)
                     /locus_tag="LTU54_000094"
     CDS             complement(9832..10755)
                     /locus_tag="LTU54_000094"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBL8294837.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            10951..11556
                     /locus_tag="LTU54_000095"
     CDS             10951..11556
                     /locus_tag="LTU54_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8294838.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            11864..12880
                     /locus_tag="LTU54_000096"
     CDS             11864..12880
                     /locus_tag="LTU54_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL8294839.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13021..14322
                     /locus_tag="LTU54_000097"
     CDS             13021..14322
                     /locus_tag="LTU54_000097"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBL8294840.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14368..15030
                     /gene="deoC"
                     /locus_tag="LTU54_000098"
     CDS             14368..15030
                     /gene="deoC"
                     /locus_tag="LTU54_000098"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBL8294841.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15051..15449
                     /locus_tag="LTU54_000099"
     CDS             15051..15449
                     /locus_tag="LTU54_000099"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBL8294842.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15515..16597
                     /locus_tag="LTU54_000100"
     CDS             15515..16597
                     /locus_tag="LTU54_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8294843.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            16809..18371
                     /locus_tag="LTU54_000101"
     CDS             16809..18371
                     /locus_tag="LTU54_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8294844.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18364..19497
                     /locus_tag="LTU54_000102"
     CDS             18364..19497
                     /locus_tag="LTU54_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294845.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19497..20453
                     /locus_tag="LTU54_000103"
     CDS             19497..20453
                     /locus_tag="LTU54_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294846.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            20552..21718
                     /gene="deoB"
                     /locus_tag="LTU54_000104"
     CDS             20552..21718
                     /gene="deoB"
                     /locus_tag="LTU54_000104"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBL8294847.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            21742..22560
                     /locus_tag="LTU54_000105"
     CDS             21742..22560
                     /locus_tag="LTU54_000105"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL8294848.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            22577..23284
                     /gene="deoD"
                     /locus_tag="LTU54_000106"
     CDS             22577..23284
                     /gene="deoD"
                     /locus_tag="LTU54_000106"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBL8294849.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(23582..24268)
                     /gene="gpmA"
                     /locus_tag="LTU54_000107"
     CDS             complement(23582..24268)
                     /gene="gpmA"
                     /locus_tag="LTU54_000107"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL8294850.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(24440..25021)
                     /locus_tag="LTU54_000108"
     CDS             complement(24440..25021)
                     /locus_tag="LTU54_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8294851.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(25154..25837)
                     /gene="rpiA"
                     /locus_tag="LTU54_000109"
     CDS             complement(25154..25837)
                     /gene="rpiA"
                     /locus_tag="LTU54_000109"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBL8294852.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            26156..26569
                     /gene="rpsL"
                     /locus_tag="LTU54_000110"
     CDS             26156..26569
                     /gene="rpsL"
                     /locus_tag="LTU54_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBL8294853.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVRD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            26653..27123
                     /gene="rpsG"
                     /locus_tag="LTU54_000111"
     CDS             26653..27123
                     /gene="rpsG"
                     /locus_tag="LTU54_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBL8294854.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            27217..29304
                     /gene="fusA"
                     /locus_tag="LTU54_000112"
     CDS             27217..29304
                     /gene="fusA"
                     /locus_tag="LTU54_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBL8294855.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            29476..30663
                     /gene="tuf"
                     /locus_tag="LTU54_000113"
     CDS             29476..30663
                     /gene="tuf"
                     /locus_tag="LTU54_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL8294856.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            31070..31378
                     /gene="rpsJ"
                     /locus_tag="LTU54_000114"
     CDS             31070..31378
                     /gene="rpsJ"
                     /locus_tag="LTU54_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBL8294857.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            31409..32038
                     /gene="rplC"
                     /locus_tag="LTU54_000115"
     CDS             31409..32038
                     /gene="rplC"
                     /locus_tag="LTU54_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBL8294858.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            32066..32689
                     /gene="rplD"
                     /locus_tag="LTU54_000116"
     CDS             32066..32689
                     /gene="rplD"
                     /locus_tag="LTU54_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBL8294859.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            32689..32979
                     /gene="rplW"
                     /locus_tag="LTU54_000117"
     CDS             32689..32979
                     /gene="rplW"
                     /locus_tag="LTU54_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBL8294860.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            33018..33851
                     /gene="rplB"
                     /locus_tag="LTU54_000118"
     CDS             33018..33851
                     /gene="rplB"
                     /locus_tag="LTU54_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBL8294861.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            33894..34172
                     /gene="rpsS"
                     /locus_tag="LTU54_000119"
     CDS             33894..34172
                     /gene="rpsS"
                     /locus_tag="LTU54_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBL8294862.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            34195..34542
                     /gene="rplV"
                     /locus_tag="LTU54_000120"
     CDS             34195..34542
                     /gene="rplV"
                     /locus_tag="LTU54_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBL8294863.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            34556..35212
                     /gene="rpsC"
                     /locus_tag="LTU54_000121"
     CDS             34556..35212
                     /gene="rpsC"
                     /locus_tag="LTU54_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBL8294864.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            35215..35649
                     /gene="rplP"
                     /locus_tag="LTU54_000122"
     CDS             35215..35649
                     /gene="rplP"
                     /locus_tag="LTU54_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBL8294865.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            35639..35827
                     /gene="rpmC"
                     /locus_tag="LTU54_000123"
     CDS             35639..35827
                     /gene="rpmC"
                     /locus_tag="LTU54_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBL8294866.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            35852..36118
                     /gene="rpsQ"
                     /locus_tag="LTU54_000124"
     CDS             35852..36118
                     /gene="rpsQ"
                     /locus_tag="LTU54_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBL8294867.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            36176..36544
                     /gene="rplN"
                     /locus_tag="LTU54_000125"
     CDS             36176..36544
                     /gene="rplN"
                     /locus_tag="LTU54_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBL8294868.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            36581..36889
                     /gene="rplX"
                     /locus_tag="LTU54_000126"
     CDS             36581..36889
                     /gene="rplX"
                     /locus_tag="LTU54_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBL8294869.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            36917..37456
                     /gene="rplE"
                     /locus_tag="LTU54_000127"
     CDS             36917..37456
                     /gene="rplE"
                     /locus_tag="LTU54_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBL8294870.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            37475..37660
                     /locus_tag="LTU54_000128"
     CDS             37475..37660
                     /locus_tag="LTU54_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBL8294871.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            37696..38094
                     /gene="rpsH"
                     /locus_tag="LTU54_000129"
     CDS             37696..38094
                     /gene="rpsH"
                     /locus_tag="LTU54_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBL8294872.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            38126..38662
                     /gene="rplF"
                     /locus_tag="LTU54_000130"
     CDS             38126..38662
                     /gene="rplF"
                     /locus_tag="LTU54_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBL8294873.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            38823..39179
                     /gene="rplR"
                     /locus_tag="LTU54_000131"
     CDS             38823..39179
                     /gene="rplR"
                     /locus_tag="LTU54_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBL8294874.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            39200..39700
                     /gene="rpsE"
                     /locus_tag="LTU54_000132"
     CDS             39200..39700
                     /gene="rpsE"
                     /locus_tag="LTU54_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBL8294875.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            39715..39894
                     /gene="rpmD"
                     /locus_tag="LTU54_000133"
     CDS             39715..39894
                     /gene="rpmD"
                     /locus_tag="LTU54_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBL8294876.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            39938..40378
                     /gene="rplO"
                     /locus_tag="LTU54_000134"
     CDS             39938..40378
                     /gene="rplO"
                     /locus_tag="LTU54_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBL8294877.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            40378..41673
                     /gene="secY"
                     /locus_tag="LTU54_000135"
     CDS             40378..41673
                     /gene="secY"
                     /locus_tag="LTU54_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBL8294878.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            41735..42382
                     /locus_tag="LTU54_000136"
     CDS             41735..42382
                     /locus_tag="LTU54_000136"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBL8294879.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            42573..42791
                     /gene="infA"
                     /locus_tag="LTU54_000137"
     CDS             42573..42791
                     /gene="infA"
                     /locus_tag="LTU54_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBL8294880.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            42824..42940
                     /gene="rpmJ"
                     /locus_tag="LTU54_000138"
     CDS             42824..42940
                     /gene="rpmJ"
                     /locus_tag="LTU54_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBL8294881.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            42958..43323
                     /gene="rpsM"
                     /locus_tag="LTU54_000139"
     CDS             42958..43323
                     /gene="rpsM"
                     /locus_tag="LTU54_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBL8294882.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            43351..43740
                     /gene="rpsK"
                     /locus_tag="LTU54_000140"
     CDS             43351..43740
                     /gene="rpsK"
                     /locus_tag="LTU54_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBL8294883.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            43820..44758
                     /locus_tag="LTU54_000141"
     CDS             43820..44758
                     /locus_tag="LTU54_000141"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBL8294884.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            44786..45166
                     /gene="rplQ"
                     /locus_tag="LTU54_000142"
     CDS             44786..45166
                     /gene="rplQ"
                     /locus_tag="LTU54_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBL8294885.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            45438..46277
                     /locus_tag="LTU54_000143"
     CDS             45438..46277
                     /locus_tag="LTU54_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL8294886.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            46253..47122
                     /locus_tag="LTU54_000144"
     CDS             46253..47122
                     /locus_tag="LTU54_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL8294887.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            47112..47909
                     /locus_tag="LTU54_000145"
     CDS             47112..47909
                     /locus_tag="LTU54_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL8294888.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            48112..48858
                     /gene="truA"
                     /locus_tag="LTU54_000146"
     CDS             48112..48858
                     /gene="truA"
                     /locus_tag="LTU54_000146"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL8294889.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(49313..51982)
                     /locus_tag="LTU54_000147"
     CDS             complement(49313..51982)
                     /locus_tag="LTU54_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HBL8294890.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFFYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(52220..52408)
                     /locus_tag="LTU54_000148"
     CDS             complement(52220..52408)
                     /locus_tag="LTU54_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBL8294891.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            52678..53040
                     /locus_tag="LTU54_000149"
     CDS             52678..53040
                     /locus_tag="LTU54_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL8294892.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            53092..54774
                     /locus_tag="LTU54_000150"
     CDS             53092..54774
                     /locus_tag="LTU54_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBL8294893.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            54891..56927
                     /gene="recG"
                     /locus_tag="LTU54_000151"
     CDS             54891..56927
                     /gene="recG"
                     /locus_tag="LTU54_000151"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBL8294894.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            56976..57977
                     /gene="plsX"
                     /locus_tag="LTU54_000152"
     CDS             56976..57977
                     /gene="plsX"
                     /locus_tag="LTU54_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBL8294895.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            58099..58341
                     /gene="acpP"
                     /locus_tag="LTU54_000153"
     CDS             58099..58341
                     /gene="acpP"
                     /locus_tag="LTU54_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL8294896.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            58609..59613
                     /locus_tag="LTU54_000154"
     CDS             58609..59613
                     /locus_tag="LTU54_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8294897.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            59614..60558
                     /locus_tag="LTU54_000155"
     CDS             59614..60558
                     /locus_tag="LTU54_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8294898.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            60558..61520
                     /locus_tag="LTU54_000156"
     CDS             60558..61520
                     /locus_tag="LTU54_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294899.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            61547..62461
                     /locus_tag="LTU54_000157"
     CDS             61547..62461
                     /locus_tag="LTU54_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294900.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            62487..64268
                     /locus_tag="LTU54_000158"
     CDS             62487..64268
                     /locus_tag="LTU54_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8294901.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            64616..65302
                     /locus_tag="LTU54_000159"
     CDS             64616..65302
                     /locus_tag="LTU54_000159"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBL8294902.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            65322..68903
                     /gene="smc"
                     /locus_tag="LTU54_000160"
     CDS             65322..68903
                     /gene="smc"
                     /locus_tag="LTU54_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBL8294903.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            68912..69721
                     /locus_tag="LTU54_000161"
     CDS             68912..69721
                     /locus_tag="LTU54_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8294904.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            69732..70730
                     /gene="ftsY"
                     /locus_tag="LTU54_000162"
     CDS             69732..70730
                     /gene="ftsY"
                     /locus_tag="LTU54_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBL8294905.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            70917..73184
                     /gene="gshAB"
                     /locus_tag="LTU54_000163"
     CDS             70917..73184
                     /gene="gshAB"
                     /locus_tag="LTU54_000163"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HBL8294906.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(73273..73725)
                     /locus_tag="LTU54_000164"
     CDS             complement(73273..73725)
                     /locus_tag="LTU54_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HBL8294907.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            73875..74825
                     /locus_tag="LTU54_000165"
     CDS             73875..74825
                     /locus_tag="LTU54_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL8294908.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            74818..75777
                     /locus_tag="LTU54_000166"
     CDS             74818..75777
                     /locus_tag="LTU54_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBL8294909.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            75774..76529
                     /locus_tag="LTU54_000167"
     CDS             75774..76529
                     /locus_tag="LTU54_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8294910.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHIGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            76552..77505
                     /locus_tag="LTU54_000168"
     CDS             76552..77505
                     /locus_tag="LTU54_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8294911.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(78149..78445)
                     /locus_tag="LTU54_000169"
     CDS             complement(78149..78445)
                     /locus_tag="LTU54_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HBL8294912.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            78800..79156
                     /locus_tag="LTU54_000170"
     CDS             78800..79156
                     /locus_tag="LTU54_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL8294913.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            79166..80065
                     /locus_tag="LTU54_000171"
     CDS             79166..80065
                     /locus_tag="LTU54_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBL8294914.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        25879 a        14510 c        17424 g        22518 t
ORIGIN      
        1 acttttgtat atttagtaag tagctacaca cttctatata aattcagaag ttcttctaga
       61 gtttctaaag tttgtttctt aattagctta ttgattctat aatatttttg atttatatag
      121 gaaattaaaa atattataga atcaacactc agagaacggg ttggttttta atagatttaa
      181 taagtactga taatgaaatt tctcctggtt gggaaaacga agttggttac cctgtagaag
      241 aattttttca agattacttt gcggagtatg ccgataggat aatgagagat tacgtaacag
      301 ataaaactca agaagtcatc aatcaatccc agtcattaaa tgaattatct gataaggcga
      361 aagaagaagc agttgccatt atttcttcag ggattagtga ctatattgat atgagtggtg
      421 aaatcaatta tggcgataaa gacaaaattg tacgcatgat aaaagctttt atcaaaactt
      481 ttgatgtgaa ggcagatctt aagtttaatg atacttacct agtaggaaaa ttaattaata
      541 tccttgggac agagaaagat acggaaacgt ttatttcctc tttgtctatg ttgtttacag
      601 ataagcattt ttcaactcag cttggtggag aggaactagt ggaaatcttt taaccattac
      661 gcccagcatt aataaaagta tatcaaaaaa gtactcctga tgatttttaa gattggtttg
      721 agaaataatg tgaaaacact atttttaatg ttaattatag atagagatga acgcgtcata
      781 tggttaaaag aatgaaaaag tcaaagatac actgctatat ttcactaagg atatagcagt
      841 gtatctttca tgtcgtttca ctggcagttt tttataaaag gaggtgtgca tttcagttgc
      901 ttttctttgg tagttagttg aatcgtttaa gaattaacgg atgtggcaga caggtgctct
      961 gtccaaaaac cagtaaaatc tttgatggtt ccatctttat ttagctgtgc ctgttcaaat
     1021 actgttttgt ttttaccgaa tggatcaatt gcaataattg ttccgatccc cgcacgtttg
     1081 gcagcaagta atcctgagta agcatcttcg ataacaagac aatcagccgg agctaagctc
     1141 agtttgtctg ccgctttaag aaaaatatcc ggttgcggtt tcccagggaa agagccgtcg
     1201 tcatagacaa tcttgtccca gtcaaaccag cgggctaaat cgaaaatatc aaaataaaaa
     1261 gcaacattct ctttgacagt agccgtggca atcgttaatg gaatctgttg gtcagtcagc
     1321 tggtcaagag tcgcttcaag cccttttgtc aactgaagct cgttttcatt ttgcaagcaa
     1381 agcatacgat aatagctttc tttttcttct gacaatacgg cgatctcaga ggcggtcaaa
     1441 tgctctgaaa tgaaatgttt caaaatctcc gtattcgtcc gaccatgaat attttttaaa
     1501 atatcctcgt ctgataaacc atcttttgta tgatcatgta tcatatgcag ccaagcagct
     1561 tcatgcagat gagagtccaa aaacattgtt ccgttgaaat caaatataat ccctttcata
     1621 gttacgctcc tatagtggta ttttcggttg aatcagcaat ttgcagatcg acatattgtg
     1681 catagtgctc ttgaacctcc cgcgagatct ggcgatccgt aatgagcaga tcgaaatcat
     1741 gcaaattata aaacacataa aaatcatctc gattgaattt tttattatca acgagtagat
     1801 atttcttacg ggaattattt agagcgatct gctgtgcttc tccttcttcg gtactgtaag
     1861 tagagatcgc ttcgttgtga acgccatttg aactgataaa tgcttttgta aactttagtc
     1921 ttcttaaatt gtcgttggtg atcggtccga caaatgcgcc ggtatttttc cggaaatcac
     1981 cgccaatcag tagaatttct ggtccatctg cttgagcaag aatatcaaaa actggataat
     2041 tatttgtaat gatccgaatt ttcttgttca gtaaatgcgt cgccaatagt tcgattgttg
     2101 tacctggtcc taagaaaatc gtttctccct cgtgaatcaa ggaggctgct gtggcagcaa
     2161 tttctttttt ttcttgaatc tgcagagtgg ctttttcagt gtgagaaagt tcttgatcta
     2221 aagtgtagga aagactttga gcccctccat ggacacgcag cagcttgccc gatttttcta
     2281 attcatctaa atctctgcga acggtcatat cagaaacatc taattgattg atgatttcat
     2341 taacggtaag gattcctttt ttattgacca tttctacgat agtaagcaat ctttcttttt
     2401 tcaacattgt cttcacctct aatttaagga taatctatca tataaacgga caacagtcaa
     2461 tctaattcga acaaaagtaa acatttaggg tggtttttat gtttgttaca atagatttta
     2521 ttgctgtttt ctggttttta tgattatttg tgtttgtttt tgttgacaaa tgttttggat
     2581 gcgtttacaa tgcaatcagt tagaaagagc gaaaactgat tatagatttg aggagagatg
     2641 tacatgctta aattaccaga agattttatt tttggcggag ccacagcagc ctatcaggtt
     2701 gaaggagcca ctaaggaagg tggcaaaggc gctgttgctt gggatgactt tttagaggaa
     2761 caagggcgat tcagtcccga tccggcaagt gatttttatc atcaatatgc aaaggacatt
     2821 gaattgtgtg aacgttttgg tgtaaatggt ctaagattat ccattgcttg gagccgcatt
     2881 tttcctgatg gagctgggaa accaaatcca gaaggaatcg ctttttatca tcgtgtgttc
     2941 gaagaatgta agaaaagaaa cgtaacgccg tttgtgacgt tgcatcattt tgatacccct
     3001 aaacgtttgt ttgaccatgg agattttttg aatcgagaaa caatagaagc atttgtttcc
     3061 tatgccattt tttgttttca tgaattcaaa gaagtcaagg tgtggagtac ctttaatgaa
     3121 atctatccag ttgcaaccaa tcaatatttg ttaggtgttt tcccaccagg aatcaaatat
     3181 gattttacaa agatcgtcgc ctgtcttcat aatatgatgg ttgctcatgc ccgtgtagtg
     3241 aactatttca aagaaaacga acttccgggt gaaattggtg tagttcactc attagagacg
     3301 aaatatgcgg caacagacgc gccggaagat aagcatgctg cctttttaga cgatgcattg
     3361 tcgatccgtt tcttattaga tgctacctat ttaggctatt attcaactga aaccttgact
     3421 gctcttgatg agatatgtga agcaaatcag gccagctatc attttcctga agaagatttc
     3481 gttgaattga aaaaagcatc cacgcgtaat gactacttag ggatcaatca ctaccaatgc
     3541 cattttgtca aagcttacga tggagaaaat gcaatccatc ataacggtac gggtgaaaaa
     3601 gggacctctg tttataaagt gaagggaatc ggagaacgta tttataaaga aggaattcct
     3661 cgaactgatt gggactggct gatttatcca gaaggtctat atgacttgtt gctgcgaatt
     3721 aaaagcgatt acccacatta caacaaaatc tatatcactg agaacgggat ggggtataaa
     3781 gatcagtttg aagatggcat tattatggat cagccgagaa tcgactattt gagagtctat
     3841 ttggaatcct taagcaaggc gataacagcc ggggtaaatg tgaaggggta ttttttgtgg
     3901 tcgcttatgg atcttttctc atggacaaat gggtataaca aaaggtatgg cttgttttat
     3961 gttgattttg aaacccagaa gcgttaccca aaagaatcag cttactggta caagttggtc
     4021 agtgaaacca aaacgattat ttaataagtg ataatggagg aagaaaagat gaataaagag
     4081 gaattacaaa tgcttggttt tgagatcgtt gcatattcag gcgacgctcg cagtacatta
     4141 ttgaaattat tgaaagaagt aagacaagga aactttgaac atgttgattc ggcgttgaaa
     4201 gaagccgacg aaaatttaac ccttgcacat aattcgcaaa caaaaatttt agcggaagaa
     4261 gcatctggga aagagatgga gatgggattc atttttatcc atgggcagga tcatttaatg
     4321 accactttat tgctgagaga tctgattcaa gactttattg tactgtacag acagaatcaa
     4381 ggataggaga agaacgctat ggatggaatc attaaacaaa tcgaaaaagg gaaaccattt
     4441 tttgaaaaaa tctcacgtaa tagttattta ggagccgttc gtgacggatt tttaacagcg
     4501 atgccagcta ttttattttc aagcattttc attttgattg ctgcggttcc tgagattttt
     4561 ggttaccaat ggcctgaatc tgtctccaca tggctatgga aagtctataa ttattcgatg
     4621 ggtgttgtag ggttactcgt ttcagcaacc actgctcgct gcttggctga ttcgatgaat
     4681 cgtcgcttac cgaaaaacaa agtaatcaac accacctctg tgatgctggc ctctgtggta
     4741 agtttcctgt tgttaagcgt ctctccgatt gaaggtggat tttcaacgga atatatgggg
     4801 acaaagggac tactggcttc ctttgtcgct gcatttttaa cagttaattt atataagttt
     4861 tgtgtgctga aagatattac cattaaaatg cctaaagaag ttcctggaac gatttcgcaa
     4921 accttccgag atatttttcc tttctctttt tcagtatttg cggccgtttt aattgattta
     4981 ggcattcgac aaatcttcca aatctcattt gcagaagcaa tcattacggt gctgcagccg
     5041 ctatttacag cagctgacgg gtacttagga atcgctatta tctggggagc gatggcgctg
     5101 ttttggtttg tcggtgttca tggaccgtcg atcgtggagc cggcaattgc ggcaattatc
     5161 tatgccaatg tggaaaccaa tctgcaattg ttcaattccg gtgaacaagc ttctaaagta
     5221 ctgacggtgg gtttagggaa ttttgtagga actatgggag gaactggggc aacattggtt
     5281 gttcctttta tcttcctact ctttgctaag tctaagcagt tgaaggcagt tgggaaagct
     5341 tcctttattc cagtaagttt cgctgtcaac gaaccattgc tatttgcggc acccatgatt
     5401 ttgaaccctt atttttttat tccatttttg ttaacacctg ttgtaaacgt ttgcctgttc
     5461 aagttttttg tcgatgtatt gggcatgaac agctttatgt acgttttgcc ttgggcaact
     5521 ccagcaccga tcggtttggt tctaggaacg ggcatgggta tcttagcttt tgttctagca
     5581 gcggttctaa tcgtcgttga cttcctgatc tatctaccat tttgcaaagc atacgatgct
     5641 gtcttagtag agaaagaagc acaaaaagca gaggtgatcg caaccgagga aggtgcttct
     5701 gccgatgtac gtgaggaagt acctgtggca gcgagtcaag ttatcagcga tgcaaataaa
     5761 gaaatcaatg tgttggtact ttgtgctggc gcagggacaa gtgcgatgtt agctaatgct
     5821 ttaaaaaaag gtgcatctga atcgaaagca ccgatcagtg caatggcggg tgcttatggt
     5881 tctcattacg aaatgctgca ggattttgat atggtggtct tagcaccgca agtcaattct
     5941 tactatgatg atatcaaaga agacaccgat cgcttaggaa tcaagctggt tgcgacaaaa
     6001 ggagcagaat atatcggatt aacgagagat cctgaaaaag ctgttcagtt tattctgaac
     6061 caatttcaat aaaggaggag ccataatgaa aatgggaatt tggttgcgta aagctgtttt
     6121 atttgcagct gtgttagggg ctttgggggc tgtttcaaaa actgttgaag catctgccag
     6181 cgaatttatt cgcggagcag atatttcgat cctgtcagat atggaaaaat ctggtgcaaa
     6241 atattatgaa gacgatattt caaaagatgc cttagagatt ttaaaaaata atggtgtcaa
     6301 ctatgttcga ctgcgtttgt ggcaggatcc ttataatagt aatggagaaa gttatggagc
     6361 agggacaaat gatctggaaa cgacgattga ccttgcaaaa cgagcaaaaa atctgggact
     6421 taaggtgctg ctggattttc actacagtga tttttgggtt gatcctggga aacagaatct
     6481 tcctaaagca tggcaaggtc tgacctttga agaaatgaat acggcattgt acgactatac
     6541 aaagaacgtt ctgtcggaaa tgaagcagct ggacgtttat cctgatatgg ttcagatcgg
     6601 caatgaactg aatagcggaa tgctctggcc ttatggcaag tcttggggag aaggcggggg
     6661 agaatttgat cgattggctg cttttttgaa gtctggtatc caagctgttc gagacactca
     6721 acctaaaaca actccagtaa tgctccactt agctgatggt ggcgatacag gagcatttac
     6781 ttggtggttt gatgaaatca cgagtcgagg tgtgtcattt gatttaattg gagtgtcgta
     6841 ttatccgtat tggcacggtt caatgaatga acttcaaacc aatatgaatg cgatcagcca
     6901 acggtatggt aaagaagtga tcgtagttga aacagcttat gggcatacaa cagcgaatgc
     6961 cgatactatg ccaaatgctt ttggtgaagc cgaagcagct gcggggggat atgaaccaac
     7021 accgacagga caagcagagt atttgctgga tcttgccgat cggattcagg cagtaccgaa
     7081 taatcgcggg gcgggcttct tctattggga acctctttgg tacaacggaa atgtgagttg
     7141 ggcaacacaa gcaggtatga attatctcag tgttcaatcc actatgggaa acgaatggga
     7201 caaccaagcg atgtttgatt ttgctggaaa cgccttgcca agcttgcgtg cgtttaaaca
     7261 agcaggggcg caaataaatc tagttaaaaa tgcaagtttc gaaagcgatg gaccaacgac
     7321 cagtccttcc aattggcaaa cctggtttca acaaggaacc gctaagggta cgatcaaaac
     7381 ggaagcgggc actttttttg gggactacaa actaactttt tggcaggatc aagcatacga
     7441 ggcatcagct tatcaaaatg ttagtggcat aagcaaagga acttacacat tgagtgccta
     7501 tgtaatgtct gggggagagt ttgatactaa tcagttatat atcaaaaatt atggtggagc
     7561 ggaactaaat actgcggttg ttaaaacaag tgaatggaca aaaattacaa tcaaagacat
     7621 tccaattaca acaggtgttt gtgaaattgg ggtttatagc aaggcttcag gaaatgcttg
     7681 gacgaacgtt gattttgttt cattggtgaa acaatagact gcggatcgtt tcgattcaag
     7741 atcggaggtt cccttcagcc acatcgttag cgtagaaggc aggaatcgaa cctgcacctc
     7801 gcctttgcca aaaataacca gaaacgattt ccagtcattt ttaccaaaac gagtaaagca
     7861 ccagacttca tctacacttt gtattatgct aaagagagtt caacgatttc gtaagaagca
     7921 atgaaaaaat tgcaaaaagt tgtgcagata acataaagtt tgtttaagaa agggtaacct
     7981 tagaattccg tcgtgaatat gatttgagca cttagatgga tagaaaaaag ttctcggaaa
     8041 acagttcgtt tttcgagaac ttctttttat gataagaatg aaacaaatta acttatatta
     8101 tagtctcttt tggtagggaa ccatcttatt tttaatgaaa atcaagcatt ttagctattg
     8161 aagaatgttg atttataggg attccccctc catattatgt tggctcttta ttcccactcc
     8221 actgttgctg gtggtttaga agtgatgtca tacacgatac ggttgacgtg ggctacttcg
     8281 tttacgatac gtacagagat tttttgcaag acttcccatg ggatacgggc aaagtcagcg
     8341 gtcattccgt cgattgaagt tacagcacgg ataccgactg tgtagtcgta agtacgtccg
     8401 tcacccataa caccgacaga gcggatccct ggaagaacag tgaaatattg ccagatatca
     8461 cggtcaagtc cagcagctgc gatttcttca cgcaagatag catcagattc gcgtacgatt
     8521 tctagcttgt cttcagtgat ttcgccaaga acacgaatac caagaccagg accagggaat
     8581 ggctgacgcc aaacgattga atcaggcatg cctaactgag tccctaattc acggacttcg
     8641 tctttgaata atgtgtttaa tggttcgatc aattcaaatt gcatatcttc tggcagtcca
     8701 cctacattat ggtgagattt gatcgtctgt gctgtttctg taccagattc gataacgtca
     8761 gtatacaacg ttccttgtgc taggaaagag acgccttctt caccagcaag ttttgttgct
     8821 tcgtcatcaa aaacatatac gaattcattc ccaatgattt tacgtttttg ttctgggtca
     8881 gatacacctt tcaacttgtc taagaagcga tctcttgcat tgactttgat gatgttcaag
     8941 ccgaattttc cgcctagact ttccattact tgttccgctt cgcctttgcg tagcagaccg
     9001 tggtcaacaa agatagatgt caattggtca ccgatcgctt tttgtaaaag aaccccaacg
     9061 acactagaat caacgccacc tgaaagacca agcaagactt ttttgtcacc aacttgttca
     9121 cggatttttt ctacttgcat atcgataaag ttatccattg tccagttacc ttcacagtga
     9181 caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt attcggaatg acgtacttct
     9241 gcatggaatt ggataccata aaaattacgt gttgtgtctt ggatagaagc aatcggacaa
     9301 tctttgcttg ttccgactgt ttcaaagcct tcaggaactt tagttaccaa gtcaccatgg
     9361 ctcatccata ctgtttgttg ttttggtgtt tctttaaaca agacagcttg atcgcttgtt
     9421 acttctaaca aggctttccc atattcgcgg tttttagcag gctctacgga accgccaagt
     9481 ttgtacgtga tcaattgcat accatagcaa atccctaata caggaatacc taaattgaag
     9541 atctcaggat cgatcccaaa tgcattctca tcatatacac tgtttggtcc gccagaaaga
     9601 atgattccct ttggattcat cgcttttact tcttctgcag taatgcggtg gctcaataat
     9661 tcggaaaaga ctccaagatc acgaatacgt cttgtgatca attggttata ctgactaccg
     9721 tagtctaata cgataatctt ttcaacgttc gtcatatcgg caacgtttgt caaatttatt
     9781 acccctatcc ctttaaattt tatctcaaaa tagtcaaact attttaaaat gctaatattt
     9841 tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta tggagaatta tatcggctct
     9901 tcctcgtgta ggcaagatat attcctgtaa atttttcaaa ttcacatttt tccatacatc
     9961 ttttgccatt ttaaaggctt cttcacgatc tccgatagca aaacgataat aataattctc
    10021 tggattttgg aaagccgtat ccagtaacgc accaaaacga tccaaatacc atttttcgat
    10081 cagcacggga tcggcatcga catagacaga aaaatcaaaa aagtcgctga cgtagatttg
    10141 ctgattcgca ggtaattgaa gcgtattgat cccctcaacg atcaagatat caggctgctg
    10201 gatcaattcg tacttgtctg gaatgatatc atacacatca tgtgagtaaa caggtacttt
    10261 tatctcttcc tgaccagatt ttacacggtt caagaaatcg atcagcatct ccatatcata
    10321 actttcagga aatcctttac ggtccatgat tccacgctct tttaatatac gatttggata
    10381 aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt ttgaaggtac gagacaaaat
    10441 cgtctgcagc agtctggctg ttgtactttt tccaacggct acacttccgg cgatcccgat
    10501 aataaaaggc ggtacttcta cgtatttatg aagaaataaa cctttactca agaacaaaga
    10561 ttcaaattct ttcatataaa gataaatcag atgagcaaga ggtacataga tatctcgaac
    10621 atcctgcatg gaaatgcggt cattcaaact tttgatatta tctagttcat cttgtgtcaa
    10681 aggtgcttgc ccattacgat aaaattctcg ccattcttct cttgccacac ggtagtagtt
    10741 cattggatca ttcatattag cctcctgcgt tctcccatca atggaactat tttaacacaa
    10801 aataaacgca aaccccacca cttaagagag attgccgatc tttttgtata atctttataa
    10861 tagaaagaaa cggagtctta ttcttgactt gcgcacggat acataaaagg atagactgtt
    10921 gtcatgaaag aaatgagaag gatggaagac atgacaaatc attactattc tgaacgccca
    10981 gatacggcgc acgattttga acaatgggct tttgagttaa aaggcagaac ctttcaattt
    11041 gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt atggttcacg tgtcctgatt
    11101 gatgctttcg attgggaaga gttacctgat gaaggaaaga tcttagatgt gggatgcggg
    11161 tatggaccga ttgggttagc tttggcattt gcgacacagc ggtttgttga aatggtcgac
    11221 attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa agcgtaacca aatcgaacat
    11281 gttgatatcc atcaatcgaa tatctatgaa gcagttcatg aagaaaccta tgcagctatc
    11341 gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac atgaaatttt gacaggcgca
    11401 tatcctcggt tgaagaaggg cggtacactg acaattgtga tccagaagaa acaaggagcg
    11461 ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg cagaaatcgt cacaaaagat
    11521 aaaggatatt atatcatcaa aagtgtgaaa gaataactag ctgaaaaata gtggagcgac
    11581 gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca gttctttttt tgttgagaag
    11641 tttttaaaaa caaagaacta ttttttcttc aaaaagaaat agtaggaaca gagtcgttgt
    11701 tttttacgat aaaataaata agatacagaa cttgtataga aggctgccaa gtcggtagat
    11761 aaaggaggtt tttatcaaaa aacatacttt ccttttttct ctattctttt cttgggggtt
    11821 ttggtacact gatagggaat ttagatagaa gggagccaat aggatgaagc tgaccatcag
    11881 agatatcgca gaaatggcag gcgtatcagt aacaactgtc tcacagattt tgaataacaa
    11941 aggaagcaga tttagtgata agacaagaaa aaaagtgttg tcgatcgtga atgaacatca
    12001 ttataagccc gattatttcg cttcaaatgt gatcaatcga cattcaaaga ccatcggaat
    12061 gatcgtccct gatgtcactg atttcttctt ctccaaactg atcgaaggtg tagagagcta
    12121 tttgaatcct ttgggctatg tgatcatgtt atgcaattct cgtcatagcc aagaaaatga
    12181 gatcaagtat ttacaagagc tttcccaccg ctcagtggac ggaatcttgc tggccacacc
    12241 aaatatttta cctgagaaat atgcattaga cagcggtttt tatcaaaaaa tgccaatcat
    12301 tttaatcgat cgcggattga atcgtcggga taacggacgg cttatcgtaa aggaatatga
    12361 gggcgcctat caagctgttt cgctgtttat tgaaaatggc catacgaaga tcggcatgct
    12421 gaaggaaacc actggatact atcaattaga agaacgtttc aacggatatc gtcatgcgct
    12481 gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa catggagagt tgactgtcca
    12541 aggtggatat actgcatcaa aaaaattact caaacataaa gatatcacgg ctattttttg
    12601 cgggaatgat gccatggcga tcggatgcta tcaggcgatc gatgaattgg gcaaaaaaat
    12661 cccagaagat atctcagtca taggattcga tggattaaag ctgtcagaat acatgattcc
    12721 gcaattgacg acagtgcagc agccaagttt tgatattgga ttttatgctg cacgtttcct
    12781 gatcgatacg atcgaatttc ctcaaagaaa agttccgaac aaagtgttcg aaacaaaatt
    12841 aattattcgt gagagcgtaa aagtattgac aaagggctaa ctcggcggta tattgcttat
    12901 gtaagcattt acaaaggcta gttagtgctt tttttatttt tagtaaacca gtttactaaa
    12961 gtggtttacc tttcctaaaa ataatgtata ataccgaatg aagacaagga ggatcaggca
    13021 atgcgaatgg tggatctgat tgaaaaaaag agggatggaa acgagctgtc gaaagaggaa
    13081 atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc cagattatca agtcagtgca
    13141 ttattgatgg caatttttta tcaagacatg acaaatgaag aaatcacgaa cttaacattg
    13201 gcgattgcta attctggtga tgtcatcgat ttgagttctc ttgaaggaat aaaggtagat
    13261 aagcattcga caggtggcgt tggtgacacg accacattga ttttagcccc cctagtagct
    13321 agtgtcggag tgacagtggc taaaatgtct ggacgcggtc taggttacac aggtggtaca
    13381 ctagacaagc tagaagcaat tcccggattt cagatcgagc tttcggatga agaatttgta
    13441 cgcattgtta atgaaagtaa agtagcggta attggtcaat caggaaactt ggcaccggcg
    13501 gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg ttgattctct tccgctgatt
    13561 gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag atgcaatcgt tttggatgtg
    13621 accactggag acggtgcttt catgaagaac atcgaagatg caagacgtct ggcaaagacc
    13681 atgacgagca ttgggaaact ggctaaccga gaaacagtgg cggttatctc agatatgtca
    13741 gaacctcttg gcgaagctat cggaaatagt ctagaagttg tcgaggcaat cgaaacatta
    13801 caaggaaatg gtccagaaga tctagtagaa atgtgctacg ccttaggaag ccaaatggtt
    13861 gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa cgttgcttca agaagctctg
    13921 gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc aaaatcaagg gggagaccca
    13981 gcgatcgtgg aacatccaga acgaattttg actgcacgct acacaatgga gcttcctgct
    14041 aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat taggaattgc tgcaatgatg
    14101 ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc acgctgttgg attgaaactg
    14161 cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac tattgacgat ctatagtaac
    14221 gacgaagaaa tcacttctgt cattgaatta ttatataaaa atattgaaat tggtgaatcg
    14281 gcaatgaagc caacgttgat tcatgacatc atcacagaat aaaggaaaaa agcggacttt
    14341 accgttttaa agaaaagggg aaaagaaatg gaactaaacc gaatgatcga ccatactatt
    14401 ttgaaagcag acgcatctaa agaagctgtg atgcaaatta tcgaagaagc aaaaaaatac
    14461 catttttatt ctgtctgcat caatccagca tgggttgcgc tagcgaaaga acaattacaa
    14521 ggtgagccag tagccgtatg tacagtaatc gggtttcctc taggagcaaa cacacccgag
    14581 acaaaagcat ttgaaacaac agatgccatc aataacggtg cagatgaagt cgatatggtc
    14641 atcaatatcg gtgctttgaa atcaaaggat gatcaacaag tccaaaaaga tatcgaagca
    14701 gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta ttattgaaac atcgttatta
    14761 gatagagaag aaatcatccg agcttgcgaa atagcaaaag ctgcaggcgc tgattttgtc
    14821 aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag aggatgtccg tttgatgcgt
    14881 gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg gtatccataa cgaagaagaa
    14941 gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa caagcgcagg agtagcaatc
    15001 gtatctggtt caactggtga aggctattaa ttttttcaaa ggagaagctc atggcagaac
    15061 tcaaacaaga atggatcaac acagctattg aagcgttaga taaagcctat gttccttatt
    15121 cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg caagatttat caaggcatca
    15181 acattgagaa tgcatcattt ggattaacga attgtgccga gcgcacagct tttttcaaag
    15241 cagtatctga aggagaaaaa acatttacgc atctagtcgt agcggggcac acccccgatc
    15301 ctatctctcc ttgtggtgca tgtcgacaag taatggcaga attctgttcc cctgatatgc
    15361 cggttacgct agtaggagac aatggtgtga cgaaggcaac gaccgttcgt gcactgctgc
    15421 cttatgcatt tactgaaaaa gatctataac tcatgttcat tcagtcagct gactgttgaa
    15481 aaataaaaca attattttag gaggctttac agaaatgaaa aaagcaaaat tatttggttt
    15541 aggtgctgtc gcactagcag cagggttatt tttaggtgca tgcggcaaca acggctctac
    15601 tgattcatcc ggtggcaaaa gctcatcaga cacaacaact gcagcattga tcacagacac
    15661 aggtggtgta gacgaccgtt cattcaacca gtcagcttgg gaaggactag aaaaatgggg
    15721 gaaagatcaa ggactttcac gaggaaatga tggcttccaa tatttccaat cttcaaatga
    15781 atcagattac atcccaaata ttgaccaagc attgaatgct ggattcaaaa cgatttttgg
    15841 tattggttat aaactaaaac cagcgatcca agaacaagca acaaacaata caggtacgaa
    15901 ctttgttatt atcgatgatg tcatcgatgg attagataac gttgtatctg caactttcaa
    15961 agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct tatacgactg aaacaaacgt
    16021 agtcggcttt atcggcggtg taaaaggtga agtaatcgat cgttttgatg ctggattcaa
    16081 agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa atcaaagtat tgaatcaata
    16141 tgctggtgac ttctctgctc cagataaagg acgttcaatt gctcaaggta tgtatgcaca
    16201 aaacgcagat atcatcttcc atgcttcagg cggtacaggt aacggtgtat tccaagaagc
    16261 aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt atcggtgttg accgcgacca
    16321 atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa aacttcacat tgacatctac
    16381 actgaaagca gttggaacag tagtagaaga tttggcacaa aaatcagcgg acggtaaatt
    16441 ccctggcggc gaacacactg tttatggatt gaaagaagac ggtgttggtc taacagaagg
    16501 acaactttct gacgaagcga aaaaagcagt tgatgaagca aaagaaaaaa tcatttcagg
    16561 tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac catttctata gccttgttag
    16621 acacatgttt gacaaggcta tggtttgatt agaacaagaa ttgttcattt atccaagctg
    16681 ccttgtgaca gtttggataa gggaacaact ccctgaagta atgtttgttt caaaagaaga
    16741 acgctcacta agcgtgaatc ttagtcaatc gttgccctag atagaaatcg ttaggatgtg
    16801 aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca gaatattacc aaagaatttg
    16861 gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa ggcaggagaa attcatgcgt
    16921 tgcttggaga aaacggtgct ggcaaatcta cattgatgaa cgtgctttcc ggattattag
    16981 agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt aaagatcaca agtccgacca
    17041 aagccaacca attagggatt gggatggtcc atcagcactt tatgcttgtt gatgccttta
    17101 ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg cgcagggatg cttgaccata
    17161 aaaaagcacg aaaagagatc caaaaagttt ctgaacagta tggattgtca gtcaacccag
    17221 atgcttacgt tcgtgatatt tcagttggaa tggaacaacg ggtagaaatt ttaaaaacac
    17281 tttaccgagg agcagatgta ctgatttttg atgagccgac agctgtattg acccctcaag
    17341 aaattgatga attaatcgtg atcatgaagg aattagtcaa agaaggcaag tcgatcattt
    17401 tgattacgca taagttagat gaaatcaaag cagtagctga ccgctgtaca gttatccgcc
    17461 gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac ctcacaacaa ttagccgata
    17521 tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa agaagcgaag cctcaagaag
    17581 tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg tggattagaa gccgtgaaaa
    17641 acttgaactt agaggttcgt gctggcgaag tacttggtat cgctggaatc gatggaaacg
    17701 ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa ggcagaaagc ggacatatca
    17761 agctaaaagg ggaagacatc actaataaaa aaccccgaaa aatcactgaa catggtgtag
    17821 gacacgtgcc agaagaccgt cataaatacg ggttagtcct agatatgaca ttatctgaaa
    17881 atatcgcact acaaacctat catcaaaaac cttacagtaa aaacggcatg ctgaattatt
    17941 cagtaatgaa tgaacatgcc agagaattga tcgaagaata tgatgttcga acaacgaatg
    18001 aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca aaaagcaatt atcgctcgtg
    18061 aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc aactcgtgga ctggatgtag
    18121 gtgcgatcga atttattcat aaacgtctga tcgaacaaag ggacaaatac aaagcagtgt
    18181 tactgatcag tttcgaatta gaagaaattt taaatgtttc ggatcgtatt gctgttatcc
    18241 atgaaggaga aattgtcggt atcgttgatc cgaaagaaac atctgaaaat gaattaggtc
    18301 tattgatggc gggctatacc ttagaagaag cacgaaaaga gttagaaaag ggggctggcg
    18361 cacatgaatg atcgcagcga aaaattccgt aatttattag tgcctgtact ttcagttgtt
    18421 ttaggattga tcttaggcgc gatattgatg gctgcttttg gttttcagcc catccaaggt
    18481 tattcttcta tgctagatgc agcccttggt tcgcaaagag ggataggtga aacgttaagg
    18541 caagcaacgc cgttgatttt tacagcatta ggtttttctg tagctaactc agctgggttt
    18601 ttcaatattg ggctttctgg acaagcactt tgcgggtgga tcgtcagtgt ctgggtggca
    18661 cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt tatgcgtgat cttaggtgcc
    18721 ttagctggtg cgatggcagc ggctgttcca ggtttacttc gagcattttt tggaacaagt
    18781 gaagtgatcg taacaatcat gatgaactat atcttattat acgtaagcac atttgcttta
    18841 catgaagtga tgcctgaaag ctatcgctca agtttggact cttccaatat gatcagtcaa
    18901 aatgcctcac tgcggttacc atggttaaca caagcttttg gcggctctcg cgtaaactca
    18961 ggattatttt tagcaatcat cgcgcttatt ttcgtttggc tgctgatgaa gaaaacgact
    19021 ttaggatttg aaatccgttc tgttggattg aatccttttg cttctgagta tgcaggaatg
    19081 agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg gcgctttggc agggcttggc
    19141 ggtgtggttg aaggattagg aacgtatcaa aacttctttg ttcaaacgac ttcgctatct
    19201 attggatttg acggaatggc tgtttccttg cttggttctg gatcagcaat cggtattctt
    19261 ttatcagcga tcttgttcag tattttgaag atcggtggac ttggcatgca gacaggagca
    19321 ggtgttccgt ttgagatcgt caatgtatcg attgctttga ttatcttctt tgtcgggatc
    19381 aacttcctga ttcgtttcgt catggctaaa gtcttccaag ggaaaaaaca agaagagatc
    19441 gttgcgacaa tagaatcgaa accaacgaat caaaaccatg aagggggcga actataatgt
    19501 cgactatcga attaattgcc ttgatcctta cttctacact ggtttattca acaccgctaa
    19561 ttctaacgtc actaggcgga actttttctg aacgaagcgg gatcgtaaac gtagggctag
    19621 agggaatcat ggtcatgggt gcttttagtt ccatcgtttt caacttgacg atgagtccat
    19681 cacttggttc tatgacacca tggatcgcta ttttagtggg tggcgtcgta ggtatcctat
    19741 tttctctgat tcacgcagta gcaacagtca gtttgcgtgc tgaccatatt atcagcggga
    19801 cagtaattaa tctgatggca cctgctttag gtgttttctt gatcaaagca tggtatggta
    19861 aaggtcaaac tgataacatc acgcagaatc tggggtattt ttctttccca ggattagcag
    19921 atattccggt cattggacca attttcttta aaaatacatt cttacctgct tattgtgcta
    19981 ttttgattgc tgtcctagct tgggtgatca tcttcaagac gaaattcggc ttgcgcctcc
    20041 gttcagttgg tgaaaatcct caagctgccg acacattagg tatcaatgta tatgctatgc
    20101 gttattcagg cgtactgatt tctggcttgt taggcggtat gggcggtgct gtttttgctc
    20161 agtcgatttc cggtaacttt tcagctggta cgatcgttgg acaagggttc atctccatgg
    20221 ctgctatgat ctttggtaag tggaatcctt taggggcaat gggtgcctca ttgttctttg
    20281 gttttgctca aagcttgagt atcattggcg cacaattacc agttatttct tcgattccgc
    20341 cagtattgct gcaaattgct ccatatttgc tgacaatcat tgtgctggtt gttttcttag
    20401 ggaaagcttc tggtccaaaa gcaaacggga agaactatat caaatcaaaa taatctttta
    20461 gcgaataaaa agggtgggtt acgggaagtg tttttcgtct cgcccttttt gttttaaagg
    20521 caataaaaat aaaaagaaag aaggaataat catgtttaaa cgcgtacatc tgatcgtcat
    20581 ggattcagta ggaattggag aagcaccaga tgccgataag ttcggtgatg ttggttctga
    20641 tacattagga cacattgcga aagaagcagg attgaacgtt ccacatttag agcagcttgg
    20701 tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg aaagatcatc aaggctatgc
    20761 gacgaaatta gaagaagttt ctgtagggaa agatacgatg acaggccact gggaaatcat
    20821 gggattgaat atcaaaactc catttcgtgt gtttcctaat ggattccctg atgatctgat
    20881 caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt aacaaacctt atagcggaac
    20941 agctgttatt gacgattacg gtgaacacca aatgaaaaca ggcgatttaa ttgtatatac
    21001 ttctgccgat cctgtccttc agatcgcggc acatgaagaa atcattcctt tagaagaatt
    21061 atatcggatt tgccaattcg ttcgtgatat aacaaaagat gatccgtata tgattggccg
    21121 aatcattgca cgtccgtacg tgggtgaacc aggagacttc acacgaacaa gcaatcgcca
    21181 tgattatgcg ttgaatccat tcggacacac agtccttgat tctttgaaag aagcaggaaa
    21241 ggacgtgatt gctgttggaa agatcaacga tattttcaac ggacaaggaa tcactgaatc
    21301 tgtccgcacg aaaagcaata tggatggcgt tgaccagctg ctgaaagtta tgaaaaacga
    21361 tttttcagga atcagcttta ccaatttagt agattttgat gcattatacg gtcatcgtcg
    21421 ggatgtagtc ggctatgcgc atgcgattga agaatttgac ttacgaattc cagaattgct
    21481 tgaagcgatg gaagaagacg acttgctaat gattacagcc gatcacggaa acgatccgac
    21541 gtttactggt accgatcata cgcgagaata tgtaccgtta ttggtttaca gtaaaaaaat
    21601 gaaagaacaa ggggcattgc ctcaaggata ttattcagat atttcagcaa cgattgctga
    21661 gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc ttgaaattat taaaataatc
    21721 tttttaaata ggagaaagca aatgaccaag ttaagcgaga tgttacaaga aacgacccag
    21781 tttatccttg ataaaggagt aaaagaaatt gattttggtt tgattctagg ttcaggatta
    21841 ggtgaattag cagatgaaat cgaagatgct gtaatgattt cttatgatga gatcccgttc
    21901 tttcctgttt cgacagtagt tgggcatgca ggaaagctag tttatgggtc tctggcaggc
    21961 aagaaagtgt tagccatgca agggagattc cactactatg aaggtcattc catgcaaaca
    22021 gtgacctatc cagtacgtgt gatggccgga ctaggggccc attcagtcat tgtgactaat
    22081 gcatgtggtg gtgtcaacga atcatttgta ccaggagatt tgatgctgat taccgatcat
    22141 atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg aagatgcaat gggccctaga
    22201 tttccagata tgagtcaagc ttatacgcct gactatcaag caacagcaaa agcagcagca
    22261 gaaaaactag gtttggatct taaagaaggc gtatatatgg gctattctgg accaacttat
    22321 gaaacgcctg cggagatccg aatgtctcgg acaataggag ccgatgcagt agggatgtcg
    22381 actgttccag aagtcatcgt agcagctcac agcggattaa aagtgttagg aatctcttgt
    22441 attaccaact tagcagcagg tatgcaagcg aatctgagcc atgaagaagt tgtagaaaca
    22501 actcagagag tcaaacaatc ttttaaagca ttggttaaag aaacattgac attattgtaa
    22561 taggaggaac aataaaatga gtgtgcatat tgaagcgaaa gaaggcgaga tcgccgataa
    22621 gattttactt ccgggagatc ctttgcgagc taaatatatt gcggagactt ttttggaaga
    22681 tccagtttgt tacaatcagg tacgcggtat gttaggcttt accggcaaat ataaaggaga
    22741 acgtatttct gttcaaggaa caggaatggg aatgccatca gccaccatct atgcccatga
    22801 attgatccag tcgtatggtg taaagaaatt gattcgagta ggtacatgcg gtgcattgtc
    22861 aaaagacgtc catgttcgtg atttagtact agcacaaggt gcagcgacca gttcttcgat
    22921 gatcgaaaaa aatttccaag cctttcattt tcctccaatc agtgatttca atctattact
    22981 gaaagcatat gagatagcta aagaaaaggg ctatacggtt catgtaggaa atgttctttc
    23041 ggaagattct ttctacaaag atgatttgac agagacgttc caattagctg aacttggcgt
    23101 tttaggagtg gaaatggaag cagcggctct ttattactta ggcgcgaaat accatgtgca
    23161 gacattaagt ttgatgaccg ttagcgacca tttgatcacc ggtgaagaaa caacagctgc
    23221 agaacgtcaa tctacgttca atgaaatgat cgaagtagga ttggaaacag cgatagcaga
    23281 ataagaagga aatgaaaaag ctgccctgca ttaagtaata agaacatcca agcaaaaata
    23341 gctcctcatg tttttgactt ggctggactt attattgcaa atgtaagctt ttgaacaccg
    23401 tttaccaaga ggttgtgaat ctgccgttta actctgagtc acaaggaaca atttcaaaaa
    23461 acagcttttc atgtttgatg aatattgtga cttgtgaccg agaagttggc aggagaaaac
    23521 cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca gtcccttttt tatagtgttt
    23581 tttataggta gtatttttta gcaactgtta agtcatcatt taattcataa acaagtggtt
    23641 gacctgttgg aatttcaaga tccatgatgt cttcgtcaga gataccttcg atatgtttag
    23701 ctagggcgcg taatgagtta ccatgtgcag ctactaaaac agttttgttg tctagtaatg
    23761 ctggagcaat ttcatcttgc cagaatggca aagcgcgttc taatgtcact ttcaagtttt
    23821 ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg acggtcattt gctgcagaac
    23881 cttcgtcagt ttcttccatt aatggaggaa gagtatcgta agaacgacgc cagatgtgca
    23941 cttgttcgtc gccgtatttt tcagctgttt cttttttgtt caaaccttgt aatttaccat
    24001 agtgacgttc gtttaagcgc catgatttga tttgtggtac ccaaagttga tctgaatatt
    24061 ctaagatcaa gttacatgtt ttgattgcac gagttaaaac agaagtgtaa gcaacatcaa
    24121 attcgattcc agcttctttg attttgcgtc cgccttcttt tgcttcttcg attccttctg
    24181 gtgctagatt tacatcagcc caaccagtaa attggttcaa tgcattccat tcactaagac
    24241 cgtgacgaga aaaaactaat tttggcatgt ttagctctcc ttttaagata ggtttatatt
    24301 tctgaaattt tcagttctgt tttattgtac aattttttgg aagagatttc catcgaaaaa
    24361 ttagataaga ttctatgaga aaacaagtag agaatataaa aaaacgaaca gtgcccacgc
    24421 aactgttcgt ttttaggtat tagatgcata attgctcgat ttctttttca ggtaaattga
    24481 tatatttata acatgttcca actgaaacac cgcattttga agaaataaat tggatcgttt
    24541 cttttttttc ataatagaga cgtcggattt ttttgatcgt ttttgcatcg atttttggtc
    24601 ttccgccgat ttttcctttt ttacgagctt catctaatcc aactaatgta cgttctttga
    24661 tcaatgcgca ctccatgttt gctaggtttt ccattaattg gaaatagatt tttcccattt
    24721 cgcgcgtatc gattccttca tcaagactaa tcaagtgcag cccgcgatct ttgaataatt
    24781 gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg atgaagttgg caaatgacta
    24841 gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa atgaacagca gtagaatcat
    24901 tttgttccac ttcatatgtc tcatggaaaa tttcttgaca accaaagtca gataattgat
    24961 caacctgagt tgttaaatca acgtcattta tggttgtgcg tgcataacca ataattttca
    25021 tggtgattcc tttctagcag tgattttcta actggatata acagtaaaaa tgtagcataa
    25081 agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg tcttttttta ttggatgatc
    25141 aagcagtttt tctttattta cgacggattg cgtcaagaat ctcaacgcct ttttctgagc
    25201 cgacaactac tttagaaacc atgtttagga aaagcccatg ttctactacc ccaacaagtt
    25261 catctaaata ggtagctaag ctttctggct gttcgatgac ttctagatgc agatcaatga
    25321 tgtaatgacc accatctgtg gttaagactt ctccgttttc gtctgtacgc aagacgggtt
    25381 ggaatctttt ttcttcaaaa atatgaagta gctgttggga gccataaggg atcacttcaa
    25441 ctgggagtgg gaacttccca agtttatcga ctaatttaga agaatcaacg atccaaatcg
    25501 tttcttttga gtacgtagcg actattttct cgaaaagcaa agcagcgccg ccgcctttga
    25561 tcccttgaaa atcagcactg atttcatcag caccgtctat tgtcaaatcg actacgggga
    25621 cttcatcaat actctttaac ggtattccta atttttccgc ttgttctttt gtcgcatttg
    25681 aagtagtaac tcctgtgata cgtaaacctt cttcacgcat acggcgacca atctcttcta
    25741 ccatataata ggcggttgaa ccggtcccga gacctacgat catcccgtcc tttacgaatt
    25801 tcgcggcttc aatgccagct aattgtttta aattcatctt tgcgtccacc tttttttagt
    25861 ttgagcatcg tgctttcctc actattttag cgtgatttac ggtaaaaaga taccattctt
    25921 ttttattcca ataaagcttt ttcaaaaaaa attgcatctc ttgttgacat gttttttcat
    25981 tcgtgatatt ctaacaaagg tgctttatct acaggtttct gtgaagaaac gtgctgactg
    26041 tttatcaaag catatttgat aaggatgcag acattgcatt tattttttat cacaaaaaaa
    26101 gaaccacctg gatgtgtgga actagacgca aatcgtggga aggaggaaac aaggaatgcc
    26161 tacaattaac caattagtac gtaaacctcg taaatcaaag gtggaaaaat ctaattcacc
    26221 tgctttgaac aaaggatata acagctttaa gaaaactcaa actaacgtaa actcacctca
    26281 aaaacgtggg gtagcaactc gtgtgggtac aatgacacct aaaaaaccga actcagcttt
    26341 acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa gttactgctt atatcccagg
    26401 gattggtcat aacttacaag aacacagtgt tgtattatta cgcggtggtc gtgtaagaga
    26461 cttaccaggg gtacgttacc atatcgttcg cggtgcttta gatactgctg gtgttaacga
    26521 ccgtaaacaa agccgttcta aatacggaac taaaaaacct aaagcttaat tttaattatt
    26581 aaacaaatga aacttgcttc tactatatag aagtaattac tatgaacaat atttcggaag
    26641 gaggagttac ggatgccacg taaaggtcct gttgcaaaac gtgatgtttt accagatcct
    26701 atttataact caaaattagt aactcgcttg atcaaccgtg taatggttga cggaaaacgc
    26761 gggattgcag ctaacattat ctataatgcc tttgacttga tcaaagaatc tacaggtaac
    26821 gatccattag aagtgtttga acaagcaatg aaaaacgttc aacctgttct tgaagttaaa
    26881 gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg aagttcgccc tgaacgccgc
    26941 gttactctag gtcttcgttg ggtagtaaac tacgctcgct tgcgcggtga acacacaatg
    27001 gaagaacgct tagcaaaaga aatcatggat gctgcaaaca acacaggtgc ttctgttaaa
    27061 aaacgtgaag acactcataa aatggctgat gccaaccgtg cgtttgctca ctatcgctgg
    27121 taagattctc tctgtttggt cggcttttat aagtcgacct cagatagtgt ccttattttt
    27181 agcaactaga tagtaaagag aggagaaaca aggagaatga ccagagaatt ttctttacaa
    27241 aacactcgta atatcggtat catggcccac atcgatgctg gtaaaacgac tgctactgaa
    27301 cgtatcttgt actatactgg taaaatccat aaaattggtg aaactcatga aggagcttca
    27361 caaatggact ggatggaaca agaacaagaa cgtggtatta cgatcacttc tgctgctact
    27421 acagcacaat ggaagggtca ccgaatcaac atcatcgaca caccaggaca cgtagacttc
    27481 acagttgaag ttgaacgttc attgcgtgta cttgatggtg ctgtaactgt tcttgatgca
    27541 caatcaggtg ttgaacctca aactgaaaca gtttggcgtc aagcaacaac ttatggtgta
    27601 cctcgtatcg tatttgcaaa caaaatggac aagatcggtg ctgacttctt atattcagta
    27661 tcaactttac atgaccgttt gcaagcaaac gctcacccaa tccaattacc aattggtgcg
    27721 gaagacgact tcacaggaat tatcgacctt gtaacaatga aggctgaaat gtatacaaac
    27781 gatcttggaa cagagatcga agaaacagaa attcctgaag aataccgcga attagctgaa
    27841 gaatggcgcg aaaaattggt tgaagctgta gctgaaactg atgaagaact tactttgaaa
    27901 tacttagaag gtgaagaaat cactgaagct gagttaaaag aaggtatccg ccgcgcaaca
    27961 gttaacgtag aattctaccc agtactatgt ggttcagcat ttaagaacaa aggggttcaa
    28021 ttactattgg acgctgttct tgattacttg ccatcaccgt tagatatccc agctatcaaa
    28081 gggattgacc ctaaaactga tgaagaagta gaacgtcctg cagacgacag cgctcctttc
    28141 tcagctttag catttaaagt tatgactgac ccattcgtag gtcgtttaac tttcttccgt
    28201 gtgtactctg gtgttttaca atcaggttca tacgtacaaa acgcaacaaa aggcaaacgt
    28261 gaacgtgttg gtcgtatcct acagatgcat gccaactctc gttcagaaat ctctgaagtt
    28321 tacgctggag atatcgctgc tgctgttggt ttgaaagaca caactactgg ggacacacta
    28381 tgtgatgaaa agagtctagt tattcttgaa tccatggaat tcccagaacc agttatccaa
    28441 gtcgctattg aacctaaatc aaaagctgac caagataaaa tgggtgtggc tttacaaaaa
    28501 ctatctgaag aagatcctac tttccgtgct gaaacaaacg tggaaacagg cgaaacaatc
    28561 atcgctggta tgggtgaact tcacttagat atcatcgttg accgtatgcg tcgtgaattc
    28621 aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc gtgaaacttt ccgtgctggc
    28681 actcaagccg aaggtaagtt tgtacgtcag tctggtggta aaggtcaata cggtcacgta
    28741 tggattgaat ttacaccaaa tgaagaaggt gctggtttcg aatttgaaaa tgcgatcgtc
    28801 ggtggggttg ttcctcgtga atacattcca gctgttgaaa ctggtttgaa agatgctatg
    28861 gaaaatggtg tcttagctgg ctatccatta gtagatatca aagctaaact ttacgatggt
    28921 tcataccatg atgtcgattc aaatgaaact gccttccgtg ttgctgcttc tatggcatta
    28981 cgtgcagcag ctaagaaagc taatccagta atccttgaac caatcatggc tgtagaagtt
    29041 gttatccctg aagattattt aggtgatgtt atgggtcacg taactgctcg tcgtggacgt
    29101 gttgaaggta tggaagctcg cgctggcggc caacaagttg ttcgtgcgat ggttccactt
    29161 gctgaaatgt ttggttatgc tacaactctt cgttctgcta ctcaaggtcg tggtacattt
    29221 acaatgacat ttgaccacta cgaagatgta ccaaaatcag tacaagaaga aatcattaag
    29281 aaaaacggcg gaaaagctga ataatttctt gaaaccggat agtcctgcaa agagattatc
    29341 caaagagact atatttttaa tatagtttcg tgtaaaataa ttatcgatga tatgggcatg
    29401 tggtgtacct tgtgtacatc atatacctat cagacaaata aaaaatatat tgtattttta
    29461 ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt ctaaaccaca cgttaacatt
    29521 ggtactatcg gacacgttga ccatggtaaa actacattaa cagctgcaat cacaactgta
    29581 ctatctaaga aaaacggcgg ccaagctatg gcttacgatc aaatcgatgg tgctccagaa
    29641 gaacgcgaac gcggaatcac aatctcaaca gctcacgttg agtatgaaac agatacacgc
    29701 cactacgctc acgttgactg cccaggacac gcggattacg ttaaaaacat gatcactggt
    29761 gctgctcaaa tggacggagc tatcttggta gtttctgctg ctgacggccc aatgcctcaa
    29821 actcgtgaac acatcctatt gtctcgtcaa gttggtgttc cttacatcgt tgtattcttg
    29881 aacaaagtag acatggttga tgacgaagaa ttactagaat tagttgaaat ggaagttcgt
    29941 gacctattaa cagaatacga attccctggt gacgatgttc ctgtagttgc tggatcagct
    30001 ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa ttcttgaatt aatggctgca
    30061 gttgacgaat acatcccaac tccagaacgt gacaacgaca aaccattcat gatgccagtt
    30121 gaagacgtgt tctcaattac tggacgtggt actgttgcta caggtcgtgt tgaacgtgga
    30181 caagttcgcg ttggtgacga agttgaagtt gttggtattg ctgaagaaac ttcaaaaaca
    30241 acagttactg gtgttgaaat gttccgtaaa ttgttagact acgctgaagc tggagacaac
    30301 attggtgctt tactacgtgg tgttgcacgt gaagacatcc aacgtggaca agttttagct
    30361 aaaccaggta caatcacacc tcatacaaaa ttctctgcag aagtatacgt gttgacaaaa
    30421 gaagaaggtg gacgtcatac tccattcttc actaactacc gtccacaatt ctacttccgt
    30481 acaactgacg taacaggtgt tgttgaatta ccagaaggaa ctgaaatggt tatgcctggc
    30541 gacaacgtaa caatggaagt tgaattaatc cacccaatcg ctatcgaaaa cggtactaaa
    30601 ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg tcgttacaga aatcaaagct
    30661 taattcactt aagttttaaa gtaaagcaac atttattcgg acgtaatcgc tcggaacgaa
    30721 ggtcttagga tctttgttcc gagctttttt atttattgca ggatcaggaa aaaagacagg
    30781 agtattataa atagaagaaa cacgaaataa aagagtcagt attttctaaa tgaaacttta
    30841 tgaaaacaaa atgtttttta caagaaaaag ccttgctttg tgagtttata tatattataa
    30901 tgacaaaggt gcgtttactt tagtgaattg cacagacgta atcagaatga tttcgtcggc
    30961 taagaaacga gaggttgcga cacgcccgga cgctttgcca cgaacgagcg tgacggaaat
    31021 tttcgtggag ctatgtctac ttttcaaaat aggcgaagga gggaacaaga tggcaaaaca
    31081 aaaaattcgt atccgtttaa aagcgtatga acaccgtatt ttagatcaat cagcggataa
    31141 aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct ggtccaattc cattaccaac
    31201 tgaacgcagt ctttacacaa tcatccgtgc gactcataaa tacaaagatt cacgcgaaca
    31261 attcgaaatg cgtacacaca aacgtcttat cgacattgtg aacccaacac caaaaactgt
    31321 tgatgctttg atgaagctag acttaccatc tggtgtaaac atcgaaatca aattataaaa
    31381 ctaaaacaaa aataatggag gtgtactcat gaccaaagga atcttaggga aaaaagtggg
    31441 aatgacacaa atcttcactg aatctggtga attaattcca gtaactgttg tagaagctac
    31501 tccaaacgta gtactacaag tgaaaacaat ggaaacggat ggctacgaag ctattcaagt
    31561 tggttaccaa gatatgcgtg aagttttatc aaacaaacct gcgaaaggtc atgttgcaaa
    31621 agcaaacacg gctcctaagc gcttcattcg tgaattcaaa aatgttgagc taggagaata
    31681 tgaagtagga aaagaaatta cagtagatgt tttccaagca ggagacatta ttgatgtaac
    31741 aggtactacg aaaggtaaag gattccaagg ggttatcaaa cgccacggac aaagccgcgg
    31801 accaatggct cacggttctc gttaccatcg tcgtcctggg tcaatgggtc cagttgcacc
    31861 taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg ggtggcaacc gcgtaactat
    31921 ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat gttatcttga tcaaaggtaa
    31981 tgttcctgga gctaaaaaat ctttaattac tatcaaatca gctgtgaaag ctaaataatt
    32041 gtaggaagaa aggaggaact aaggaatgcc gaatgtagca ttattcaaac aagatggaag
    32101 ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga attgaaccta atgaaagtgt
    32161 tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga caaggaacac acgcggttaa
    32221 aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg cgtcaaaaag gaacaggtcg
    32281 tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga ggtggcgtag tctttggacc
    32341 aacaccacgt tcttacagct acaaacttcc taaaaaagtt cgtcgtttag caatgaaatc
    32401 tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca atcgaaggct tgaacttcga
    32461 cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac ttatctatcg actctaaagt
    32521 attggttgtt ctagaaaaag gtaacgactt tgctgcttta tcagcacgta acttaccaaa
    32581 cgtttctgta gtaacttctg ataatgtgag tgtgctagac gttgtatcaa atacaaaagt
    32641 gttagcaaca caaactgctc ttactcaaat tgaggaggtg cttgcataat gaacttacta
    32701 gacgtaatca aacgcccagt gatcactgaa aaatcaatgc ttgccatgga tgacaagaaa
    32761 tacacttttg aagtggacac tcgcgcaaac aaaactttag taaaacaagc ggtagaagct
    32821 gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg tacgtgcgaa attcaaacgt
    32881 atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag cgatcgttac attgactgaa
    32941 gattcaaaag aaatccaatt attcgaagct gctgaataag cactaataga taactcatta
    33001 aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc tacctcaaat ggtcgtcgta
    33061 acatgacagg ttctgatttt gctgaaatca caacatcaac acctgaaaaa tcattgttac
    33121 aaccattgaa aaaccatgct ggacgtaaca acaacggccg catcactgta cgtcatcaag
    33181 gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa acgtaacaaa gataacgttg
    33241 ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc agctaacatc gcgttagtac
    33301 actatgaaga tggagtgaaa gcttacatct tagcacctaa aggactagaa gtgggtatga
    33361 aacttgtttc aggtccagaa gcagatatca aagtcggaaa tgctcttcca ttagaaaaca
    33421 ttccagttgg tactgttatc cacaacattg aaatgaaacc tggtaaaggt ggacaattga
    33481 tccgttctgc tggtacaagt gctcaagtac ttggtaaaga aggcaaatac gtattgatcc
    33541 gcttgaactc tggtgaagtt cgtatgatct tagctgcatg ccgtgctaca atcggttctg
    33601 taggtaacga acaacacgaa ttaatcaaca tcggtaaagc tggacgctct cgttggatgc
    33661 gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa tgatcaccca cacggtggtg
    33721 gtgaaggtaa agctcctatc ggacgtaaag caccagtttc tccttggggt caaccagctc
    33781 ttggttacaa aactcgtaac aaaaaagcta aatcagacaa acttattgtt cgtcgtcgca
    33841 aaaataaata attactttga ccatgtgtcg cacattttga gaggaggttc accatgggtc
    33901 gtagtttaaa gaaaggacct tttgtcgatg agcatttaat gaaaaaagtt gaagctcaag
    33961 caggggttga aaagaaaaaa gttatcaaaa cttggtcacg ccgttcaaca gttttcccaa
    34021 gttttgttgg atataccatc gcagtttatg atggacgtaa acatgtacca gtttacatcc
    34081 aagaagacat ggtaggacac aaattaggtg aatttgcacc tacaagaact tatcgtggac
    34141 atgctgctga cgataagaaa acgaaacgct aatttgaggg gaggataaat ccaaatggca
    34201 gaacaaatca catcagctaa agcaactgca aaaacagttc gcacttcacc tcgaaaagcc
    34261 cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag atgcaatttc aatcttgaaa
    34321 ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat tgatgtcagc agttgcgaat
    34381 gcagaaaata actttgactt agatgttgaa aacttggtag taagcgaagc ttttgttaac
    34441 gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag gatcagcttc tccaatcaac
    34501 aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat aaggagggat aatcagtggg
    34561 tcaaaaagta catccaattg gaatgcgtgt aggcatcatc cgcgactggg atgctaaatg
    34621 gtatgctgaa aaagagtatg ctgagttctt acacgaagat ttaagaatcc gtaaatttat
    34681 ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa atcgaacgcg caacaggtcg
    34741 cgtaaacatt tcaattcaca cagctaaacc aggtatggtt attggtaaag gcggttctga
    34801 agtcgaaagc ctaagaaaag aattaaacaa attaactggt aaaagaatcc atatcaatat
    34861 cgtggaaatc aaaaaaccag atctagatgc aaaattagta ggtgaaggaa tcgcacgtca
    34921 attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa gccatccaac gctcaatgcg
    34981 tgctggcgct caagggatca aaactcaagt atctggtcgt ctaaacggag cagatatcgc
    35041 tcgttcagaa ggatactcag aaggaactgt tcctcttcat actttacgtg cggacattga
    35101 ttacgcatgg gaagaagcag atacaacata cggaaaacta ggagttaaag tgtggatcta
    35161 tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa ggagggaaat aatcatgtta
    35221 gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa aaatgcgcgg ggaagctaaa
    35281 ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag ctgttgattc acattggatc
    35341 acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc gttacatgaa acgtggtggg
    35401 aaagtatgga ttaaaatttt ccctcacaaa tcttatactg ccaaagcaat tggggtacgt
    35461 atgggttctg gtaaaggggc acctgaagga tgggttgcac cagtaaaacg tggtaaaatc
    35521 atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg aagcgttacg tctagcttct
    35581 cacaaattac caatgaaaac taagatcgta aaacgtgagg aaatgggtgg tgaatcgaat
    35641 gaaggttaaa gaaatcagag aattaaccac tgccgaaatg cttgatcaag aaaaacaatt
    35701 aaaagaagaa ttgtttaatc ttagattcca attagcaaca ggtcaattag aaaacactgc
    35761 acgtattaaa gaagtacgta aatcgattgc acgcatcaaa actgtgttgc gtgaacaagc
    35821 taagtaatta tggaaggagg ccattaagcg tatgactgaa ggaagaaatc aacgcaaagt
    35881 ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca attaccgttg ctgtcgaaac
    35941 aaagaaaaac caccctatct atggcaaacg catgaactat tctaagaaat acaaagtgca
    36001 tgatgaaaac aacacagcaa aagttggcga catcgtgaga attatggaaa ctcgtccatt
    36061 atcagctaca aaacgtttcc gtttgttgga agtagtcgaa gaagcagtta ttatctaata
    36121 acacgagatt ttcctatata gattgaatgc aaaatctgaa aggaggatac acaacgtgat
    36181 ccaagcagaa agtcgtttaa aagtcgctga taactcaggc gcacgtgaaa ttttgacgat
    36241 caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc ggtgatgtga tcgttgctac
    36301 ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt gacgtcgtta aagccgttat
    36361 cgttcgaaca aaatcaggag ctcgtcgtac agacggttca tatattaaat ttgatgaaaa
    36421 tgctgcagtg attattcgtg atgataaaag ccctcgtgga acacgtatct ttgggccagt
    36481 tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt tcactagcac cagaagtttt
    36541 ataatcttga gacacttatc aaaggaggtg cgaaacagtt atgtttgtta aaaaaggcga
    36601 taaagttaaa gttatcactg gaaaagacaa aaacaaagaa ggcgttgtac tagcagcgtt
    36661 cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc gttaaaaaac accaaaaacc
    36721 ttctcaagct gctcctcaag gtggaattgt tgagatggaa gcgccaattc atgtttctaa
    36781 cgtgatggtg gttgactcta ctggtgtagc tggccgagtt ggctacaaag aagtagacgg
    36841 caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta gataaataaa agtttaagga
    36901 aggagggtct tactgaatga accgcctaaa agaaaaatat cttaaagaag taactccatc
    36961 attgatggaa aaatttaact atagctctgt tatgcaaaca cctaaagttg aaaaaatcgt
    37021 catcaacatg ggtgttggtg atgcggtatc aaacgctaaa aacttagata aagcagttga
    37081 agaattaact ttgatcactg gtcaaaaacc aatgattaca aaagctaaaa aatcaatcgc
    37141 tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa gttacattgc gcggagaaag
    37201 aatgtacgaa ttcttagaca aattggtttc tgtttcacta ccacgtgtac gtgacttcca
    37261 tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat actttaggta ttaaagaaca
    37321 attgatcttc ccagaagtag attatgattt agtagataaa gtacgtggta tggacatcgt
    37381 tattgtaaca acagcaaact ctgatgaaga atctcgtgag ttgttgacac aattaggcat
    37441 gccattccaa aaataattaa aggaggcgaa ctacgtggct aaaaaatcaa tgattgctaa
    37501 aaacaaacgt cctgcaaaat tttcaacaca agcttatact cgttgcgaac gttgcggacg
    37561 tccacattca gtttatcgta agttccatct ttgccgtatt tgcttccgcg aacttgccta
    37621 taaaggtcaa attcccggcg tgaagaaagc tagctggtaa aaagtaaatt cgcataagaa
    37681 ggaggtaaaa gatcaatggt catgacagat ccaattgcag attttctaac tcgtatccgt
    37741 aacgcaaaca tggttaaaca tgagtcttta gaagtacctg catcaaaaat taaacgtgac
    37801 atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg ttgaatacat cgaagatgac
    37861 aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa acggcgaacg tgttatcaca
    37921 aacttaaaac gtatctctaa accaggtttg cgtgcttatg tcaaagctga cgaagtacca
    37981 aaagtattga acggtttagg tatcgcaatc atttcgactt ctgaaggtgt tgtcactgat
    38041 aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg cttacgtatg gtaatcaagt
    38101 agaatctaca aggaggtgtc tttaagtgag ccgtatcgga aataaagtcg ttgttatccc
    38161 tgaaggagta acagttactc aagacggaaa caacgttaca gttaaaggac caaaaggtga
    38221 attgactcgt actttttcag cagatatcaa aatgaatatc gaaggaaacg aagtaacttt
    38281 cactcgtcca aacgacagta aagaaatgaa aacaattcat ggaacaactc gtgcgaactt
    38341 caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa gcgttagaac taatcggggt
    38401 tgggtaccgt gcacaattac aaggatcaaa acttgtattg aacgttggtt actcacatcc
    38461 agtagaaatc acaccaccag ctggagtaac tgttgaagta ccagctaaca cacaagtaat
    38521 tgttaaaggt gcgaacaaag aagaagttgg cgaattagct gcaaacattc gtggaactcg
    38581 tcctccagaa ccttataaag gtaaaggtat tcgttatgtt ggcgaatatg tacgccgtaa
    38641 agaaggtaaa actggtaaat aatcgtttag aaaaattgca tagaagccag cagcaggaaa
    38701 aaggcatttg gctgaagttt accttcaaaa aaatacattt ttacttgtgc cgcagcttct
    38761 atttttcgaa acgtatttac tgcttgaaat agtagtacaa taaaaataaa gaggtgacaa
    38821 ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa gagacatcgt cgtgtacgta
    38881 acaatatctc tggtactgct gagcgcccac gcttgaacgt ttttcgttct aacaaaaaca
    38941 tctacgctca aattattgat gacgtagcgg gtgtaacgct agcaagtgcc tctaccttgg
    39001 ataaagaaat ttcaggtgga acaaaaacag aagcagcaca agctgtcggt aaactagttg
    39061 ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt tgaccgtggt ggataccttt
    39121 accatggccg tgtgcaagct ttagctgaag ctgctcgcga aaatggacta gaattttaga
    39181 agaaggagga ataccattca tggtttatat cgatccaaaa catttggaat tagaagaccg
    39241 cgttgttgca attaaccgtg taacaaaagt tgttaaaggt ggacgtcgtc tacgttttgc
    39301 tgctttagtt gttgtcggtg acaaaaacgg acacgtagga tttggtacag gtaaagctca
    39361 agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg aagaaaaact tagttgaagt
    39421 acctatggtt ggttcaacaa tcccacacga agtaatcggt gcttttagtg gtggtagaat
    39481 cctaatgaaa cctgctgtag aaggttctgg ggttgctgct ggtggaccag ttcgtgccgt
    39541 attagaatta gctggggtag cagatatcac ttctaaatct ttaggctcaa atacaccaat
    39601 caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg aaacgtgcag aagaagttgc
    39661 agaacttcgc ggcaaatcag ttgaagaatt aatcggataa ggaggacaac aataatggct
    39721 gaattaaaag ttactttaaa acgcagtatt atcggacgtc ctcaaaacca acgcgataca
    39781 gttaaagcgt taggtcttac aaagattaac agttcagtgg ttaagccttc taatgaagca
    39841 attaaaggca tgatcaatac tgtttctcac ctagtggacg ttgaagaaat ttaatttaga
    39901 aaaatcataa ctagaacatt aaggaggtgc caaacctatg aaacttcatg aattaaaacc
    39961 tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt ggtacttcat ctggtaacgg
    40021 aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt tcaggcggcg gtgtgcgttt
    40081 aggatttgaa gggggacaaa caccattgtt ccgtcgttta ccaaaacgag gattcacaaa
    40141 cgtgaaccgt aaagactatg cagtcattaa tcttgatgta ttgaaccgct ttgaagacgg
    40201 agctgaagta actcctgttg ctttagttga agctggaatc gtgaaaaacg aaaaagctgg
    40261 aatcaaagta ttagctaatg gggaactaaa caagaaatta actgtgaaag cagcaaaatt
    40321 ctctaaagca gctcaagaag caattgaagc ggctggtgga tcaatcgagg tgatctaatg
    40381 tttagactat taaaagatgc ttttaaagta aaagacatta gatcgaaaat cctatttact
    40441 gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta ctgtaccagg cgtaaatgct
    40501 gcttcattgc aaaacctttc ttctttgcca tttttcaaca tgttgaactt ggtaagtggt
    40561 agtgcgatgc aaaacttttc cattttctca atgggggttt caccatacat tactgcttct
    40621 atcgtcattc agcttcttca aatggacatc gtgcctcgat ttgttgaatg gtcgaaacaa
    40681 ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat atctgacatt agtattggca
    40741 tttatccaat ctgtcggtat cactgctgga ttcaatcaat acgctcaatt aggtttcgtg
    40801 aataatccta acgtagcaac ttacgtaacg atcggtttga ttctaacagc cggtacgatg
    40861 tttgttactt ggctaggaga acagatcaca gaaaaaggta tcggaaatgg cgtatcaatg
    40921 atcatttttg caggtatcat ttctagattg cctgaaagta tcagagaaat ttatgaagat
    40981 tactttataa atattgatcc ttcagacata tggaaatctg ctatttttgt ggccatttta
    41041 ctagtagcgg tcttgttgat cgtgacattc gtaacatttt tccaacaagc tgaacgcaag
    41101 atcccgatcc aatatacgaa acgcgtagct ggcgcgccta caagcagcta tctgccgttg
    41161 aaagtaaatg cagctggagt tattccagtt atctttgcta gttcgtttat tgctacgcca
    41221 aatgctgttt tacaagcatt gagcggtaga ttcggttctg aaaactggta caaagtgatg
    41281 atggagatct ttaactataa tacagcacct ggtgctacga tttatacgat tctgatcgtt
    41341 gccttcactt tcttctacgc ctttgttcaa gtaaaccctg agaaattagc ggaaaacttg
    41401 caaaagcaag gaagttatat accaagcgta cgccctggta aggggactga agagtatgtc
    41461 tcaagattac tgatgcgttt aagtacagtt ggtgcagtct tccttggttt agtggcattg
    41521 cttccaatca ttgctcaaat ggtttgggat cttccacaat caatcggatt aggcggtaca
    41581 agcttactga tcgttatcgg tgtagcttta gaaactgcga aacagttaga aggcttaatg
    41641 ctgaaacgta aatatgttgg attcattgag taagaaagcg tgttgaggtt tcccctcaac
    41701 acacgttctc acacatttat taggagggaa aacaatgaac ctcattttaa tggggctgcc
    41761 aggcgcaggt aaaggaacac aagctgaaaa aatcattgat gcttatggta ttccgcacat
    41821 ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa acagctcttg gcctggaagc
    41881 aaagtcttat atggataaag gtgcattagt tcctgatgaa gtaacaaatg gaatcgtgaa
    41941 agagcgacta gctgaaccag atacagagaa aggcttctta ttagatggct ttccacgtac
    42001 gctagatcaa gcaaaagctc tggacgcaat gctaaaagat ttgaataaaa aaattgatgc
    42061 tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa cgtctagccg gccgatttat
    42121 ctgcagcaat tgtggagcaa cttaccataa aattttcaac cctacaaaag ttgaagacac
    42181 atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa gatgataaac ctgagacggt
    42241 taaaaatcgt ctggctatca acatcaaaaa tagtgaacca atcttggctt attataaaga
    42301 acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc gatgctgtat ttgcagatgt
    42361 acaaaaaatc attgaaaaat agcacgattg cccgtagccg acttgcatta tgcagttact
    42421 cttgtcaagt tcaataaatt atgatagaat atgtcagtcc gacttctaga gaatcctcta
    42481 gatgatccat tttgagaatt ttgaggtggg aactgcagtt tccgccattt tatcgtgtga
    42541 aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa agaagatatg attgaagtcg
    42601 aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa agttgaacta gaaaacggac
    42661 accaagttct tgctactgtt tcaggtaaaa ttcgtatgca ttacatccgt atcttacctg
    42721 gtgacaaagt aacggttgaa ttgtctccat acgatcttaa ccgtggccgt attacttatc
    42781 gctttaaata attgtactcc gtaaaactac aggaggtatt atcatgaaag taagaccatc
    42841 agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt aaaggacgcg ttatggtgat
    42901 ttgcccagca aatccaaaac ataaacaacg tcaaggataa ttggaggtgc tataaatatg
    42961 gctcgtattg caggagtaga tattcctcgt gataaacgtg tagtagtttc acttacttat
    43021 atctatggta ttggtaacac tactgctaaa aagatcttag ctgatgctgg tgtatctgaa
    43081 gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg ctatccgtgc agaagtagat
    43141 aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact tgaacatcaa acgtttgatg
    43201 gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat tgcctgttcg tggacaaaac
    43261 acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg ctatcgctgg caagaaaaaa
    43321 taattcttaa gtgaaggagg ttaaaacttc atggcagcaa aaaaagtgaa tcgtaaacgc
    43381 cgtgtgaaga agaatataga agcaggtatt gcacatatcc attctacatt caataacact
    43441 atcatcatga tcactgatac tcacggaaat gcattagcgt ggtcatcagc tggttcatta
    43501 ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc aaatggcagc agaaactgct
    43561 acaaaagcag caatggaaca cggattaaag actgttgaag taacagttaa aggacctggt
    43621 tctggacgtg aagcagcaat tcgttcatta caagctacag gtttagaagt gactgcaatt
    43681 cgtgacgtga ctccagttcc tcataatggt gcccgccctc caaaacgccg tcgtgtttaa
    43741 tgagtgccgc tcattttgag ttttatcagt aacgtcattg aacaagacac ttttcgtttt
    43801 gaaaggggta aagataagaa tgattgagtt cgaaaaacca agaatcgcaa aaattgatga
    43861 agaaaaagat tatggcaagt tcatcgttga acctcttgaa agagggtatg ggactacttt
    43921 agggaattcc ctacgtcgta ttttattatc ttctttgcca ggtgctgcca tcactaatat
    43981 tcaaatcgat ggtgtactgc atgaattctc taccatcaaa ggtgttagag aagacgtcac
    44041 tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg tatggccaag aagaaaaaac
    44101 ccttgaaatc gatattaccg gacctgctac agtaactgct ggcgatatta tcgttgatag
    44161 tgatgtagaa attcttaaca aagatatgta catctgtagt gtctctgaag gagctacgtt
    44221 ccatgctcgc ttaacagtga aacctggacg tggttatgtt caagcagatg aaaataaaaa
    44281 agaagatatg ccaattggcg ttcttccagt cgattcaatc tacacacctg ttcgtcgtgt
    44341 gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa gattttgata aattgacgat
    44401 ggagatttgg acagatggtt caattgaacc gttggaagca atgagtttgt ctgcgaaaat
    44461 catgactgaa catttggata tttttgtgaa cttaactgat gaagcaaaaa atgctgaaat
    44521 catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg gaaatgacga tcgaagagtt
    44581 agacttgtct gttcgctctt acaattgttt gaaacgtgca ggaatcaata ctgtacaaga
    44641 acttacaaat aaatctgagc ctgaaatgat caaagtccgt aacttaggac gtaaatcatt
    44701 agaagaagtt aaagcaaaat tgcacgactt aggtttaggt ttacgtaaag aagattaaaa
    44761 ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa ctaggacgca catctagcca
    44821 acgtaaagcg atgttgcgtg atttaacaac tgatttatta atcaacgaac gtattgtgac
    44881 tactgaagct cgtgcgaaag aagttcgctc aactgcagaa aaaatgatca ctttaggtaa
    44941 acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc gtacgtaatg aagttgccag
    45001 cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct ttacaaaaac tgttcaacga
    45061 tattgcacct cgttacgcag aacgtcaagg cggatacaca cgcattttga agacagaacc
    45121 aagacgcggt gatggtgcac caatggttgt cttagaactt gtctaaaatc acactaataa
    45181 catcacttta tagatgattc ttttagagtg ttatgatgtt ggaagatatt tttccgagtc
    45241 tagctcagcg ctgctcctca agcgaatatc gcttggggag taagcactca tcataaagtg
    45301 tttttttata ccttcaatta cctgtcatga cagatatgtt tgaaggcata aaaaaacatt
    45361 ttaagcgaga aataattgaa aattttgtct ttttttgata gtgttagtag gaactttagg
    45421 atgaagaagg agtaatcatg gagccaatta ttgaattaga aaagataaat tataaatacc
    45481 agccagatga tcttcgcccc gcattgaaag atgtctcctt tacaattgat aaaggtgagt
    45541 ggatcgctat tattggacac aatggttcag gaaaatcgac acttgccaaa acgatcaatg
    45601 gattgcttct gccagaatca gggatcgtga aagtgggaaa tcaaatattg gatgaagaaa
    45661 atatttggac gatccgacag atggttggaa tggtttttca aaatccagac aatcagtttg
    45721 ttggttcgac agtagaagat gacgttgctt ttggtcttga aaatcaaggg attcccagag
    45781 aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt tagaatggct gagtttgctt
    45841 cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg tgttgccatt gccggcgtgg
    45901 ttgccttacg acctgatatc attattttag atgaagcaac cagtatgcta gatccagaag
    45961 gtcgtgaaga agtgatctct acgatcaaga agatcaaaga agaaagtcaa ttgaccgtta
    46021 tatctatcac tcatgatatt gatgaagcag ctaatgccaa tcggatacta gtgatgagac
    46081 aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt ttctgctggt cctgaactga
    46141 tcgacttagg tctagatcta ccatttcctg aaaaactgaa gagcgcactg aaagaacgtg
    46201 gggttgatgt gcccagtgaa tatatgacag aagaaaggat ggtggattgg ttatggacat
    46261 ccgttttgaa caagtagact tcacttatca gccgaataca ccatttgaac aaagagcctt
    46321 atttgacatc aatatgacga tcaaagaaaa cagttatact gcattagtag ggcataccgg
    46381 aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt gttaagccaa caagcggtac
    46441 agtccatatt ggagaacgag atattcagcc ggatacggat aataagaatt taaagcccat
    46501 ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca cagctattcg aagaaacggt
    46561 agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc agcgaagaag aagcactagt
    46621 cctagcaaaa gaaacattag aacaagttgg gctggatgaa agctatttgg aacgttcgcc
    46681 atttgaactt tcaggagggc aaatgcgtag agtagcaatc gctggtgtgc ttgccatgag
    46741 gccggaagta cttgtattgg atgaaccaac ggcaggactt gatccccagg gcagaaaaga
    46801 aatgatggag atgttttggc gtcttcataa ggaacagcag atcactattg tattagttac
    46861 gcatttaatg gatgatgtag ccaattttgc agattatgtc tatgtgttgg aaaagggacg
    46921 aatcgttaat agcggggagc cgcaagaggt ctttcaaaac attgaatggc taaaagaaaa
    46981 acaactaggc gtacctactg ccaccgagtt tgcagaagaa ttaatggcta aagggatgaa
    47041 ctttgctacc ttgcctttga cagctgaaga attagcagac gctatcgtcc attatgctgg
    47101 aggaaatgcc catgatgaat aaattgatat ttggtcgtta tatgcctgga gattccttga
    47161 tccatcgatt ggatccccga gctaaattat tagccagttt ttattttatc ggaatcattt
    47221 ttttagcaaa taattgggaa acgtaccttt tgttggcatt tttcacatta ctttctgtct
    47281 tcttgtcaaa agtagatttg aaatttttta tccgaggcat tcgcccattg atctggctaa
    47341 tcctcttcac tgtagctttg caggtcttgt ttacaagtgg cggagaaact tattggagtt
    47401 ggggtgtttt ccatatcact gaatttggtg tgatgaacgg cctctttatt ttctgtcgat
    47461 tcgtgttgat catttttatg tcgactctac tcacgcttac tacaccgcct ttggaattat
    47521 cggatgcgat tgagtacttg cttcgtccat tgaaagccgt acggttccca gtccacgaaa
    47581 tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac attgatggac gaaacagaaa
    47641 agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg tgaaggaaac ctagttcaga
    47701 aaatgaaagc agtcattcct ttgctgattc ctttatttgt cagcagcttc aaccgtgcgg
    47761 aagatcttgc gactgctatg gaagcaagag gctacaaagg tggggaagga agaacgaaat
    47821 atcgtgtatt gcattggcat aatagagaca cgatggttat cattgctttt gtattattaa
    47881 ctgtgctact gatcttttta agaggataaa cgttcagtgg gccgctatct attcttttct
    47941 tgctgatctg tttgcaagaa aggatgcaag cagtgtatga caagaaatat tagggtctgt
    48001 ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa gaaagaacga aaaaatttta
    48061 tagaaaaatc attattccag ataacctttt aggaacaaag gagagaaaaa catggtacgt
    48121 tacaaagcaa tcattgcgta cgacggaaca aattttaatg gattccaaaa acagccaaat
    48181 gggcgtactg ttcaagaaga agtagaaaaa acattacaaa aaatggcaaa tggaaaagaa
    48241 atcacggtct ttggttcggg aaggacagat gccggagtac atgcgatcgg gcaagtaatc
    48301 cattttgatt atccagagga gcgtccgtta gaacgaatgc gtttcgcgct agatacccag
    48361 tcaccagagg atattgctgt aagacaagta gaaattgttt cagaagactt ccacgcaaga
    48421 tatttggtca aagaaaagac gtatcaattt cgagtggata tagggaaacc acgcagtcct
    48481 tttcgacgcc attatgcaag ttattttcct tacccacttg atttggaaaa aattcagcgg
    48541 gcgcttcctg atttattagg gactcacgat ttcacctctt tttgtgcatc aggaagctcg
    48601 atagaggata aaatacgtac gatctacgag gctaagatgg aggtaaatga aacaaaagac
    48661 gaattgttgt ttacatttag gggaaatggc ttcttgtata agatgatacg gatcttagta
    48721 ggaacgcttt tgaaaattgg caatggccga ttgcctgaag atagtatccc agatattatt
    48781 gcgaaaaaag atcgtaatgc agcaggacct acagcacacc ctgaaggact ttatttgtat
    48841 gaagtgaaat atgaataaga ggttatgata gaagtggtca gcttcaagaa ataagaaggt
    48901 atttacgaaa attacttctc aaaattttgc aaaatttcgg cttattttcg aagaagctac
    48961 ttctggaaca ccgtttaagg aagaggtcgt gaaatcagcg ttttgacctg agtattagag
    49021 aaacaaacgg gaaaatagct cctcatattt tgccgtttgt tgagctaata ccgaaggtca
    49081 gctgattgaa caccgtttaa gaaagaggtc gtgagcctgt catttaactc tgagtcacaa
    49141 agagcaattt caaaaaacag cttctcatct ttgatgaata tcgtgacttg tggacgaggc
    49201 gttgataagt gaatcatctt tttgttacct attttttctg taaccaaaaa acgatggaac
    49261 gttgttttga ttgcccttgg tgcggacagt ctgaacaggt tccatcgttt ctttattttc
    49321 tacgtcttct tctgtgtgtg tttcgtggtt gttcttctat tgctggatca atcgtgccat
    49381 aaaaggggcg attgcggtca aggtaccaga cataaagaat aaacaatgca atacatagga
    49441 caaaaaggat tgcacctatc gtaaatcctt caggcaagta actgaaagtg acagtatgtt
    49501 tacctgctgg aacttttatc atcaagaatg cgtctttgaa attgctgacc ttgactggtt
    49561 tcccatcaat tttagctttc catcctttgt cataaggaat agttgtcacc aacatggaat
    49621 cttcagcggt atttacagtc ccagaagctt ttctgccagt ggcttccatt tcgacacctt
    49681 tttctttgat ctgattcatt gcggcttgat aggctacaac atctagacca acgactttag
    49741 gctccatgaa acttatttct ttcgtcccat aaaaacttgc agtaaatgtc actgttgtct
    49801 cttgaggata atagccaaga tcatagtatt gaccagaaat gttgatttgc gtttttcgcg
    49861 atgtaccatt gacagtgatc gtcgcactag aactttctaa ttgcgcaaaa ttggttggga
    49921 acaaacttaa gtaagcttgt gtatttgcag gcacatggac ttcccaagtg agctcttttg
    49981 gctggttatc tgctgtttct gagtaagtga cgccagtcgt tgtctttgtg atcgtcgtat
    50041 tcaactgatt cttcaaagta ggtgtataga atgtaaaata ctgttcattt agaccagaca
    50101 atgtattgat caagtttgtc tgactggtca aattatctgt cactggctgc tcaacatcat
    50161 taacggacaa cggtgcagta aatgctagtg gcaatgcatt gacattctga tacagtgtat
    50221 agtcgccgct ttgaccagct tggcggaagc catatttact gaacgtgctt tcgtttttag
    50281 aaatgttata tttgattcct gtaaacccat ccatcagcaa agtattattg ttataacgga
    50341 tattcaaatt tgtccctctt gaacggaaac ctaatttatc caggtaagag gaagaattac
    50401 gatttcttat agaagaaaac aaactgatac cactgtaacc atagttgatc gaatcgtttg
    50461 cagatactgg atccagattt tccaagcggt aaaacgagtc ggaatctttt tttgtttcat
    50521 ttacaagagc tttcaagtca ggataaggat cagtatataa actgcgggaa gcataattcc
    50581 aatcatcaag aatgccatgg atcatactgt tcgtattgac cagtgcttca gcagacatga
    50641 tcaataataa caatatactc agatgattga atgaaagttt tttatgctga taaaatccca
    50701 cagcaaaagc ataaatccct aagaaaacca ctgtcaatac aaaggaagtc atcgtcacat
    50761 aggtgtagct tccttcaggt ttgatgcccc atgccaccgc aaagagagca gctacgacca
    50821 aaatgattcc taacaacagc ccacgatcgt cttgttctat cttttcccat ccttttcctg
    50881 caagcaggag cactaagaaa gagaacagat aggcataacg gaataaaaac atattcggtg
    50941 catgcatacc atgccagaac aaattcaatg gaacaagata aaaacttgcg ataagtatgg
    51001 cgaaaagact gccgtacaaa aatttatttt tcaacggtac tttccgactg gcaaaataaa
    51061 acagacagag gactaatggt agcagacctg cgtaaacgaa cggaatcgat ccgtattttg
    51121 tcgtgtcata aacaccaatc atatttttca tgattagatc cagataagaa gtcgcttctg
    51181 ttttcaacgt agtgatctga ctcagtgcct cgccatttga ccgaagatcc agcactgctg
    51241 gcaatacaat gatcattgaa gcgccgcctg ccagtaaaga agtgatacca taaggaacaa
    51301 tcgttttttt atattctgac cagtttgtca atagacgggc gataaaatat aagaaagaaa
    51361 atacgccaat cataaaaccc atataaaaac tggataaaaa caagagtaaa taactgataa
    51421 ataaaagttt cggccgttta agatccataa cacgatgaat ccccaaaatc actaatggaa
    51481 gataaaaaaa tgcatccagc cacatgatga tctccgaatg tgcagtgata aatgagatca
    51541 atgcgtaact gacgctaagt gcaacatgcc cccagcgagg aatcttgaac gtacgtgcaa
    51601 gaaaccaaaa agccaatccg gcacagccga ccttaagcaa agtcagaaca tacagtgcat
    51661 ctggcatcat ctggtttgga aaaaagaaca ccagtggcgt gaagatcccg cctagatagt
    51721 aagaaattaa agataaataa ttcaatccta gagaagcatt ccatgtataa aaaatacttt
    51781 gctttccaag aagaacattt cggaagctgg catgaaaatt agaaaactgt gaaaaggcat
    51841 cacttgccaa aatacttctg ctgcttccag gatagattcc gatacttaga taaacaagtg
    51901 ctaaaatcaa aaacggaatc aggaaactag ctgctgcgta gcgtccgttt tctctaaaga
    51961 atgactttaa attttttttc atatttattt tccaccaaat ttctatgaag ttgtattcag
    52021 tttatcatat ccttgttttt ctctgcttaa aaagagtcta gttttcacaa aaaaataaaa
    52081 tttagaataa ataacttcat aatttgttcg tataacaaaa ataaacgcaa aaaaagggta
    52141 ggaatcaaaa gtcccaccct taaaaatttt tattaaataa aaaaccgttc actatctgaa
    52201 cggtccaagt atcattcaat taaacgcgtt caacttttcc tgatttcaaa gcacgagttg
    52261 acacccaaac ttttttaggt tttccgtcga ccataacgcg aactttttgt aagttaggtt
    52321 taacagtacg ttttgttgag ttcatagcgt gtgaacggtt gtttccactt tttgtcttgc
    52381 gacctgtaaa gtaacaaact tttgccatgg taatttcctc ctttgccttg atttggagca
    52441 atagaataat tcagctcata ccagactaac atatcatata gaatgggaat ttgcaagtac
    52501 attttcatag atacaattga caattgagaa gaataattgt aaattaaaca aggttatacc
    52561 tgaaattttg caaggttttt gagtactttg ggtaactgcc ttttatttca agttgcgcta
    52621 tgataaaata tgatagaaga aaaatgggag ttttttaccc gttaaggagg ctcattcatg
    52681 gctgtaaaaa ttcaaacgtc agctggaacc attgaaatta ccaatgatgt aattgcaaca
    52741 gttgttggtg gcgccgcgac agatgtgttc ggtgttgtag gaatggccag caaaaatcaa
    52801 attaaagata atattactga gattttgcgt aaagaaaatt actcaagagg agtagttgtc
    52861 cgccaagaag ataatggtat tgctgtagat gtttatacga ttatcagcta tggtacgaaa
    52921 atctctgaag tttcccgcaa cgtccaagaa aaagtgaaat acaatctaga ggcattatta
    52981 ggcgtaactg ccaattcagt taatgtcttt gtacaaggtg ttcgtgtact gccagactag
    53041 gcaggagact ggctggtaac tcagcagtcg atctgaagga ggattatcta ggtgagtgta
    53101 acagaaataa gtgcaagcca gttccaggaa atggtccagg caggtgcgaa tcgtctgcaa
    53161 gtaaatgcag aatacgtcaa ttcattaaat gtattccctg tgccagatgg cgatacggga
    53221 acaaatatga atctatctat gactagtgga gctaaggcag tagtagattc aacctcagaa
    53281 aaagtaggag aattagcagc aatgctttca aaaggattgc tgatgggtgc gcgcggaaat
    53341 tccggcgtta ttttgtctca attgtttcgt ggattttcta agcaaattcc agatgttgtc
    53401 tcattaaatg cttctgatct tgctgcagcc ttcactcatg gagttgaaac tgcgtataaa
    53461 gcagtaatga aaccagtaga aggaacgatt ttgacagtag ctagagaagc agcgaaagct
    53521 ggagaaaaga aagcaaaaag tactgatgat gtcgttgaag taatgacagc tgttgtaaaa
    53581 ggagcaaagc gtgctttagc taaaactcca gacttgcttc cagtcctaaa agaagtcggt
    53641 gtcgtagaca gtggagggca aggactgctg tttgtttatg agggcttttt aagtgcattg
    53701 aatggagaat tccaagcgga tgaaacatat gaaccttctc cagcagaaat ggatgatatg
    53761 gtcaatgcag aacatcatcg cagtgtccaa ggtcaaatgg cgacagaaga tatccatttt
    53821 ggttactgta cagaaatcat ggtaaaaatc ggagaaggac ctacagtcga tagtacattc
    53881 gattacgaag aattccgtaa ctatttagat ggtataggag attctttact tgtggtcaat
    53941 gatgacgaga tcatcaaagt ccacgtgcat acagaaaacc caggagaagt cttgaattat
    54001 ggacaaaaat tcggttcatt agtcaaaatc aaagtcgaca acatgcgctt gcagcatgag
    54061 acgattttgg aacacgatga agaagtgaac gagtttgcga aaaaagaaat accacgtatc
    54121 ccgtatggta tcgtagcgat cgctgctggt aaaggcgtcc aagagctgtt tgaaagttta
    54181 ggcgctaatt atgttatcag tggtggacaa acaatgaatc caagtacgga agacattgtt
    54241 aaagcgattc aagaagtaaa cgcagataaa gtgattgtct tgccaaacaa taagaacatc
    54301 ttcatggcag ctgatcaagc agcagaagta gcagatatcc cagtagcagt tgttccttca
    54361 agaacgattt cccaaggatt gacagctatg ctttcattca atgatcaagc ttctttagaa
    54421 gaaaacaaag aaacgatgac agaaatgttg tctagcgttg tgagcggtca agtcactcac
    54481 gcgatcagag atacctcaat tgacggtgta gagatcaaag aaggagactt tttgggcatg
    54541 atcgatggaa aaatcctgat ctctaaccca gatatcacag caacagcgtt aagtacacta
    54601 gaacaaatga tcaatgagga cacagagatc gtaacgatct tggcaggtga tgaaggaaca
    54661 gttgaacaag caaatgaatt tgctgagaaa ctaacagaaa aacatcctga tttggaaatc
    54721 gagatccacc aaggagatca gcctgtctat ccttatctat tttctgctga ataatttctg
    54781 tgtcagaata tctttctaac acagtattca ttcataaaag gccttcggtt agtgatcagg
    54841 tatgatcgct gaccaagtcc tttttttgtt aaaggaggga ggaaaaattt atgcgttctt
    54901 tagatgatcc aatcactatg ctggcaggag tcggagaaaa aagagcagct agtcttgcca
    54961 gcttgggcat ccaaacgatt gaagacttac tgacttatta tccttttcgc tacgaagata
    55021 ttcaggaaag aaatctaaac gagatccagg atcaagaaaa ggttactttg aaaggaatcg
    55081 tagtctcgcc tcctgtgatg aatcgatttg gttataaaaa aagccgattg caatttcgta
    55141 tgatgcaaga acatgatgtg ttcaacgttt ctttttttaa tcaaccttat ctaaaagaaa
    55201 aagtcatttt atcggaggaa atcgccgtat acggtaaatg ggatgcaaag cgtaaagcct
    55261 tgaacgggat gaagatttta ggttctcaaa caatggatga cttttcgccg atatatcacg
    55321 tcaacaaatc aattcgtcaa acgacattag ttgatctaat caaaaaaggg tttcaagaat
    55381 acggcacatt gattgaagaa aatctgccaa acaaccttgt ggagaaatat cgactgcttg
    55441 accgagcttc tgctgtaaga agtatgcatt ttccaaaaga ccacgaagaa aaccatcaag
    55501 ccaaacgacg tgtcgttttt gaagagttct ttttatttca aatgcgtctt caaggattga
    55561 aaaaagcaga aaaagcagaa acgaatggtt tggagatcct ttatgatgtc caaaaattga
    55621 aacaatttac tcaaaagcta ccttttgaac tgacaaatgc acaaaaacgt gtaacgaatg
    55681 aaatctgtcg ggatcttcgc agtccgcagc atatgcagcg acttttgcag ggagatgtag
    55741 gtagcggaaa aacagtggtt gccgcgattg cgttatacgc taccgttaca gcaggtttcc
    55801 aaggtgcgtt gatggtgcct acagaaatct tggcacaaca acatatggaa agcttgcaac
    55861 agctgtttga tcccaatgaa gtaaccatcg cattgattac ggggtcaacg aaagcaaaag
    55921 aacgacgtga gctccttgaa aaactagagc aaggggaaat taacgtcgtg atcggcacac
    55981 atgcattgat ccaagatgga gtcgaatttc agaatttagg tctagttatt acggatgagc
    56041 agcaccgttt tggcgtgaac cagcggaaag tacttcgtga aaaaggctgg cgaccagatg
    56101 tcttatttat gacagcgact ccgattccta gaacattagc tattacagca tatggtgaga
    56161 tggatgtatc tatcattgat gaacttccag ctggacgtat cccgatcgaa acacgctggg
    56221 taagaacacc tcaattggac agtgtactgg attggacgaa aaaagaactg gcaagaggac
    56281 atcaaatgta tgtcatctgt ccgctgattg aagaatcaga agcattagat gtaaaaaatg
    56341 cagtggaaat ctatgaaaaa cttcgtgaac tatttgcacc aaaatatgaa gttggcttgt
    56401 tacatggtaa aatgaagaat caggaaaaag aagtaatcat ggaagccttc aaagacaacc
    56461 agatgcagat attagtttcg actactgtaa ttgaagtagg ggtaaacgtg ccgaacgcga
    56521 ccgtcatgct gatcatggat gcggatcgat ttggattggc acagctgcac caacttcgtg
    56581 gacgtgttgg acgcggaagt gatgcttcct actgtatctt agttgctaat cctaaaaatg
    56641 aactaggtgt ggaacggatg aaaatcatga cagagaccac caatggtttt gtactaagcg
    56701 aaaaagatct ggaattacgt ggtcctggtg aagtatttgg cttccgtcaa tctggtctgc
    56761 ctcaatttgc gattgctgac ttggtgacgg atggaaatgt tttggaggta gccagagaag
    56821 aggctgcagc cctttggcaa ataaaggatt ggcagatgct tcctgagtat cagcctttag
    56881 cgcaaagcct ccaaatagaa aatcaggata atcgttattt cgattagcag actgtgctat
    56941 actttatact gatgacggaa aaaggagtgg aaacaatgaa aattgccgta gatgcaatgg
    57001 gcggagatca cgcacctcaa gcaattgtag aaggcgtggc tttagcgcag caagattttc
    57061 cagaagtaga attcttactt tacggaaaag aagcagaaat caagaaatac ttaacaaatg
    57121 aaaaaaacat cacgattatc catacagacg aaaaaatcaa tagtgatgat gagccagtaa
    57181 aagcaattcg acgaaaaaag actgcatcaa tggtccttgc tgcacaagca gtgaaaaacg
    57241 gtgaagcaga tgctgtattt tcagcaggga atacaggcgc gctactagca gcagggttgt
    57301 tcatcgtagg acgtatcaaa aatatcgaac gccctggctt gatgtctacc ttgccagtag
    57361 tagggaaaga aggtgccggt ttcgatatgc tggatcttgg cgctaacgca gaaaacaaac
    57421 cagaacattt gttgcaatac ggtattttag gttcttttta tgcacgtaaa gtccgaggta
    57481 tcggacgtcc tcgtgtcgca ctcttaaaca acggaacaga agcaacaaaa ggtagtgaag
    57541 tgacgaaaaa agcctatgaa ctccttcaga atgaaacaag cttgaatttt attggaaacg
    57601 tagaagctcg tgagctactc aatggtgtgg ctgatgtagt ggtcacagat ggatttacag
    57661 gcaacgctgt tttgaaatct attgaaggaa cagcaatgaa tatgatgaat ctattaaaat
    57721 ctgctattct aaatgaaggg atcaaaggaa aaatgggcgc tatgctttta aaagacggct
    57781 tacgttcttt gaaagcagaa atggattatt ccaaacatgg cggtgccgtt ttgtttggct
    57841 tgaaagcacc agtcattaaa actcatggag caacaggccc cgatgctgtt cgctatacga
    57901 tccgccaaat ccacacaatg cttgagacag atgtagttgg acaattagtc gaacaatttg
    57961 aaaaaagcga agaatagtat ttggatatct gaagcgatcc gttccattga aaaaaaggtg
    58021 gacattcctg aaaaatgcca gtaaaattag ccatggtgta ggtaattgac aagtgtatga
    58081 ttcttggagg tgaaaaagtt gactcgcgaa gaagtattta acaaagtagc gaaaattatt
    58141 tcaaatcatt ttgagataga taccgataaa gtgacggatg aattgaacat caaagatgat
    58201 ttaaaagctg attcaattag tattatggaa ttcgttttag agcttgaaga tgaattcggt
    58261 acggaaattt cagatgaaga cgctgaacaa attgaaacag tcggtggcgc agtagattat
    58321 atcagcagcc atcttaaata atttcaaata aaagcaaaga aggataaaaa gctgcatgca
    58381 gctttttatc cttctttttt taatcttttt gacacgtaaa agtcattttt ctgataattc
    58441 tggcgataaa cagaaattat gttgcaaagt aaagcggttt tctatataat tattaataat
    58501 tcattacaca aagataagaa taacctttgt tataaaacga tttattctgt gtaatgtcct
    58561 tttttattaa gaaaaacttc catttaggaa gcagagaggg gtattcgatt gtccgataac
    58621 caattattag aagttcagaa cctacataca ggattccgac tcaaagatga ctattacgat
    58681 gcagtagatg acgtttcatt tactttagac aaaaacgaga ttttagcaat cgttggggaa
    58741 tctgggtgcg ggaaaagtac cttggctact acgattatgg gattacttga tcctaataac
    58801 acaaaaatta caggtgagat tttatataac gatctgaatt taatcgacct gaacgaaacg
    58861 ctctataata aaatccgcgg aaatgatatc ggaatgattt tccaagatcc gttgtctgca
    58921 ttaaatcctc tcatgagaat cgaagaccag atcaaagaag gactttccta tcacacaaag
    58981 atgaatgcag aacagcgtca agctcgtgca ctagaattgc tagaacaagt agggattcct
    59041 aatcctgcga gagttggacg ccaatatccc catgaactat caggcggtat gagacaacgt
    59101 gtcatcatcg caatcgcaat cgcctgcaaa cctccaatcg tcattgcaga tgaaccgacg
    59161 acagccctag atgtaaccat ccaagcacag attcttgatt tactgaaaga ccttcaggaa
    59221 gaaacaaaaa cagggatcat cttgatcact catgacttag gagtcgtagc agaaatggct
    59281 gatcgagtgg cagtgatgta tggcggacag ttcgttgaag tagcaggtgt aaaagaactg
    59341 tttgatcatc ctaagcatcc ttatacacgc tctttactga actctatacc acaagaagga
    59401 aatcatgatg cagaactcca tgtgatcgaa ggtgtcgttc cttctctgaa aaacatgcct
    59461 cgtaaaggtt gtcggtttgc accacgtatt ccatggatac cagcaagcga tcatgaagaa
    59521 gagcctatcc ttcatgaagt cgcaccgaac cacttggtac gttgctcctg ctataagcat
    59581 ttccacttta gagacgaaaa aggagaggtg tagatgacag aattaatcac aatcaaagac
    59641 ctgaaagttc attaccctat tcgcagtgga ttcttcaatc gagtaacgga tcatgtatat
    59701 gctgtagatg gtgtggattt tattatcgaa aaaggaaaga catatggttt agtgggagag
    59761 tctggctctg gaaaatctac tacgggaaaa gccgttgtgg gattagaaaa ggtaacagcc
    59821 ggacaaatca tgtatgaagg aaaagatgta acgaagcgaa gcaaccgcaa aaaaatgggt
    59881 tataacaagg atgtgcagat gatcttccaa gattcaatgt ccagtttgaa tcctaaaaag
    59941 cgagtgttgg atatcattgc tgaacccatc cgaaattttg aacggttaag cgatcaagaa
    60001 gaaaagaaaa aggtaaaagg actgctagat atcgttggga tgccggaaga tgcgctatat
    60061 aaatatcctc atgaattttc aggcggacaa cgtcaacgtc taggagtagc ccgtgctgtt
    60121 gctaccaatc caaaactgat cgtagccgat gaacctgtat ctgctctgga tctatctgtt
    60181 caagcgcaag tattgaattt catgaaacga atccagcaag aatacggcct cagctattta
    60241 ttcatttcac atgatttagg tgtagtaaaa cacatgtgcg acaatatcgc aattatgtac
    60301 aaaggtcgat ttgtagaaat cggtacaaga gaagacattt ataatgaccc gcgacatatc
    60361 tatacgaaac gtctgctttc agctattccg caaatcgatg tagaaaatag ggaaagccac
    60421 aaagaaaatc gacgaagagt agaacaagag tatatccaaa accaaaaaga atactatgat
    60481 gcgactggtc gagtctacga tttgcgtacg atcacaacta cacacaaagt ggcgttgaaa
    60541 gatggaggtg caagctaatg tggaaaacga ttctgcgacg tgttttattg atgatcccgc
    60601 aagttattat tttaagtgtt ctgatcttct tattagccca agcgatgccg ggggatccat
    60661 ttaccggttt gatcaatcca aatcaggatc cggcagtgat cgaacagatg cgacaagcag
    60721 ctggcttgaa tgacccatgg tatgtccagt acttccgttg gatcggaaat gctttgcaag
    60781 gcgattttgg tcaaagtttc ttatacaaac gctctgttgc tgacttgatc ggcgaacgga
    60841 tcatcaacac tttatatcta tcgatcttga cagttgtcct tacttatttg attgctgtgc
    60901 ctctggggat gcttgctggc cggtatcaaa attcaatctt ggataagatt gtcgtaatct
    60961 acaacttctt tagttttgct gttcctttat ttatcttcgg cttgatcatg ctatttgttt
    61021 ttggttatcg tttaggctgg ttcccaacaa gtggatcaca aacactaggt ttgcaaggtg
    61081 gatttatcac tcaatggata gaccgtttga aatatattct gttaccatca attacccaag
    61141 catttttggc gactgccgtc acaatccaat atctgcgaag tgaagtaatc gattcgaaat
    61201 cgctcgattt tgtcagaaca gcccgttcca aaggggtacc gacgaataaa gtattcagtc
    61261 gacatatttt ccgaaatgca gcgttgccga tggcttctca aatggggtat gaaatcactt
    61321 cgttgattgc tggttctgta gttatcgaaa agatttttgg ttatccggga gtcggaaaac
    61381 tatttattga ttccatcggc caacgggact ataccgtgat cacatcctta gtattgattt
    61441 taggaatcgc gacgctagtg gggacattac tttcagatat catcatgagt atcgttgacc
    61501 cgcgtattcg gattcaataa aaccaatcag aaaggagaat ggatcgatga gtgaaaaaaa
    61561 caatacagca gttcaagaaa gtattcctcc tatggggata cgaatgattg ctcgagaatt
    61621 taaaaaagat aaaattgcta ttttttcatt aggtttgcta gtggttttat tattagtgat
    61681 ctttatcggt gcgatgctga ccgatcaaga caaagtgatg tatgttagta tcttcgacaa
    61741 atacgctgca ccaggaacag tttcgacaca aggacaaaag ttcattctgg gagcagatga
    61801 aggcggacgt gatgtattag gacaattgat cataggtgct agaaactctg ttacgatcgg
    61861 atttgcgatc acgatcctta cttcgatcat cggggttgga ttagggatca tctctggcta
    61921 ctacggaggg atcattgaca acatcttgat gcggatcgtt gattttatca tgattctacc
    61981 gatcatgctg atcattatcg tcttcgtatc gattatttcc agatacaatg tctggtcctt
    62041 tatattcatc atgagtgcat tttattgggt agctaaagcc cgattattcc gaagcaagac
    62101 attatctgaa gcaagaagag attatgtcag tgcctcaaaa acaatgggaa ccaatgattt
    62161 taaaatcatg ttccgcgaaa tcatgccgaa tctaagttca ttaatcatta ctaatttgac
    62221 gatgaacttt gcggcaaaca tcggtatcga gacaactttg actttcttag gtttcggctt
    62281 gccggcaaat gtacctagtt taggtacatt gatcggttat gcaagtaacg gagaagtctt
    62341 agtgaatcgg caatggatct ggttacccgc atcaatcctt attttagttt tgatgttgag
    62401 tatcaattat gttggacaag cattcaagcg ttctgcagat gcgcgccaac ggttaggcta
    62461 gtatagaagc gaagggggaa ataggaatga agaaaacgat ttttggattt gcagcgctga
    62521 tgtctgctgt cgttcttgca gcatgcgggg gcggaggtac agatacctct ggaaacaact
    62581 ctggaggcaa cggcaataac aaagaggaat taacttttcc aattgcaaca aaaaataatg
    62641 atgatgcaat cgaagacggc gaattagaag ctgcggtcgc aacggacagt cagtttaaag
    62701 gattgttcca gtgggagttt taccaagatt catatgatgc agcttttatg gctccttctc
    62761 atgaaccgct atttgcaaac gatgacgatt tcagaatcac agacgacgga gctgcaacgc
    62821 ttgatctaga tgaagatacg aaaaaagcga caatcaaagt aaaagacaac gtcaaatggt
    62881 cagatggtga agatgtaacg gcggacgatg tgattttccc ttacgaaatc attgggaata
    62941 aagattacac tggtatccgt tatgacgaca ctttccgtaa catcgtaggt atggaagaat
    63001 atcatgatgg aagtgccgac acaatctccg gaatcaaaaa aatagacgac aaaacagttg
    63061 aaatcgaata taaagaagta aacccaagca tgcttcaatc gggtggtggt atctggtcat
    63121 atgcagcacc aaaacatacg ttagaaggtg tagcaatcaa agatatggaa tcaagcgatc
    63181 aagtacgtaa aaatccagtg acttttggcc cttattacat gagtaatatc gttgcaggag
    63241 aatcagtaga atacttgcca aacgagcact actggaatgg tacgcctaaa ttgaaaaaga
    63301 tcacgatgaa atctgtaccg atggcaagtg caactgaagc attgaactct aaattatatg
    63361 atatgatttt ccaaatgcca acagatactt acgataccta taaagaaatt gctggctata
    63421 caaacttagg ccgtgaacaa acttcttata cttatctagg attcaagtta ggtaaatgga
    63481 atgcagatac aaagagtgtg gaatacaatc cagattcaaa aatggctgat aaatcattac
    63541 gtcaagcaat gggctatgca ttagataata atgcagtagg tgaacgtttc tatgcaggtt
    63601 tgagaagcaa tgcgacttca ttgattcctc cagtattcca gtcataccat gatgatgaga
    63661 tgaaaggatt cacacaagat ctagacaaag cgaaaaaact attagatgat gctggctaca
    63721 aagatgtgga tgacgatggt ttaagagaag acaaagatgg aaataaacta acaatcaact
    63781 ttgcttcgat gtccggtggg gaaacagcgc aaccattggc agattactat atccaacaat
    63841 ggaaagaaat tggtttggat gtccaataca caacaggtcg tctgatcgaa ttcaattctt
    63901 tctatgataa attagaaaac gatgatcctg aaattgatgt ttaccaagct gcttggaata
    63961 caggaactga tccaaatcca agtggactat ggggaccaaa tgctgcgttc aactacactc
    64021 gttatgaatc agaagaaaat acaaaattga tcaacgatat cagttcaagc aaagcatttg
    64081 atgaagagta ccaaaaagaa gcatatcaaa aatggcaaca atatgcattt gaagaagcat
    64141 ttgcgattcc aacgttgttc cgtaatgaag tattgccagt caacgaccgt gtcaaagact
    64201 gggattggtc ttatgatgta ccaaacccat gggcaaatgt ttctgttaca gcagattcac
    64261 gttcataatc actaaaaatg accgaaaaag ggcgatgtcc tttttcggtt ttttgatatt
    64321 tttctgttta tttctctaat attcaggtca aggcgctgat ttcatgactt ctccaataaa
    64381 cggtgttcaa agtccagttt ctcggtatta agtcaaattt atgcaaaaat atggaaagcc
    64441 attttcacaa aattttttct tcatactcga aactaaacga atttttcaca acttctcgtc
    64501 aatcactagg attattaaag ctttacttac cttttgaccg aaccatttca aatttgacaa
    64561 aaaaccagta taataaggaa gtatcgaaaa aatatccata aaaggaatgt ggaaaatgga
    64621 caaccagctg acaaaagaac taaaagaaaa ctataacatc gtttttcacg atctaaattt
    64681 gcttgaacaa gcttttaccc attcatccta tgtgaatgag catcgcaatc tgcaattatc
    64741 ggataatgag cgacttgaat ttttaggaga tgccgtttta gaactgatgg tttctcagta
    64801 tttattccgt ttatatcctc atttgccaga aggaaaattg acaaaaacac gagctgcgat
    64861 cgttcgcgaa gacagcttgt cgaaatttgc caaagaatgt cactttgatc aatatgtttt
    64921 gttgggcaag ggagaagaga attcaggagg acgtacgcga ccagcattgc tttgcgacct
    64981 attcgaagct tttttaggcg cgttatatct tgaccaggga ttcgacacga cccatgcatt
    65041 catcgaaaaa gtgatttttc ctaaagtcaa agcaggtgct ttttcacatg agatggatca
    65101 taaaacaaaa ctacaagaag tcttgcaaaa atcaggtgat gtatcaatcg aataccgatt
    65161 gatcaatgaa gaaggtccag ctcatgaacg agtattttgg atcgaagttt atgtagatga
    65221 tcaattgatt ggcactggac aagggaaatc gaaaaaatta gcagaacaag ctgcggcgga
    65281 aaatgcattg gcagcactgt aaaagtaagt gagggaaagc tttgtatttg aaacgaattg
    65341 aaatagccgg ttttaaatca tttgcagaca agacagtcat tgattttgaa aatagcgtca
    65401 cagcagtggt tggaccaaat ggcagcggaa aaagcaatat tacagaggct atccgctggg
    65461 tattaggaga acaatctgct aaaagtctgc gaggcggaaa aatgccggat atcatctttg
    65521 ccggttccga tacccgtaag cagctgaata ttgcagaagt cacagtgatt ttagacaata
    65581 gtgatcatta tctgccattg gagtacaatg agatcagtgt aacccgccgt tatcgacgga
    65641 ctggggaaag cgagtttttc atcaataaac aaagctgcag attaaaagat atccaagaat
    65701 tgttcttaga ttctggatta ggaaaagaat cgttctcaat catttctcaa ggaaaagtag
    65761 aagcgatttt cagtagtaaa ccggaagacc gtcgtgggat ctttgaagaa gcagctggtg
    65821 tcttgaaata caagcagcgg aaaaagaaag cagaacagaa gctttttgaa acagaagaca
    65881 acctcagccg cgtccaagac atcatccacg aattggaaga acagttaact cctttggccg
    65941 cccagagtga ggcagcaaag gagtttttac ggctaaaaga aacactgact caaaccgatg
    66001 tctctttgat ggttgctgaa atcaagacag caaaaaaaga ttgggacaac aaacaagcac
    66061 agcttgcgaa attcaatcta gaattaggca aactatctga gtcgatccaa gaacaagaat
    66121 ccattttggc gaagcagcgt aaagaaaatg cgcaagccga tcgtctgatc gaaaaaaatc
    66181 agcaagtatt attagatctt tcggaaaaac tgaagcagac agaaggacaa aaagatgttt
    66241 tgcaggagcg gacaaagcat acacaaaaaa gctcgcaaga atatcaaact tcattagcag
    66301 aagcacaaaa aaaggttaaa cattttgaaa agcttcaaga aagcttgatg aaagcagcag
    66361 cagaaaaaga aacagagatc caaaaagctg aagcaaatct cataaaaaca cagcaagaat
    66421 tagaaaaata ccaaaaatca acgaaagaat tattggcaga attaagagat caatatgttg
    66481 atttgatgca agaacaagca gctgtaggaa acgaactgaa gtatctagaa cgtcaatata
    66541 tacaagaaac agcaaaaagc aagcagacgt tagccaagca atcggaagta gaagcatcag
    66601 tagaccgatt gatcttacaa aaagaagaat tgacacaaaa gcaggcgcag ctaaaatcat
    66661 ctttaactga aacaaaagag aagttagaaa tgatccaaca aaacgggaaa aagttccaag
    66721 aagcattagc aaaagaacag cctaaaatgt atcaattaat gaatcaagtc cagcagctac
    66781 gtgctcgtaa gaaaagcctt caagaaatcc aagaaaacta ttttggtttt taccaagggg
    66841 ttcgattggt cttgcagcat aaacagcagc tttcgggtat tgtcggagca gtggctgaac
    66901 tgatcgatgt tcctgcagat ttcacgttag ctatcgaagc agctttaggt ggggcagccc
    66961 agcatgtcat cgttgaaaat gaaaaagatg ctcggcaagc catcacgtat ttgaaacagc
    67021 aaagaggagg aagagcaaca ttccttccac tgacgacaat caaaccaaga caattaccgg
    67081 ctcatatatt gacacaagca gcagctgtag aagggtttat agggattgcc agtgaacaag
    67141 tcagttatcc cgatcaaatc caaactgtgg ttcaaaacct gctaggaacg atccttttag
    67201 ccaaagactt aactagtgcc aatgcaatcg cgcaaacgat ccgctatcaa tatcgagtgg
    67261 tttccttaga aggtgatgtg atgaatgccg gaggatccat gacaggtgga gcgaataaac
    67321 gaggaaacca aggcagtttg tttgtacaaa atcaagaatt gaaacagttg actagtgaat
    67381 ttgaagaagc agataaacaa ctccaagcac aagaaaaaaa agttcaggag ctgcaacaag
    67441 aaacagctcg tttagccgaa gaacaagaag ttttgcgtac acgaggcgaa caattacgtt
    67501 ttgaagaaca agaagcgaca aatcagctac aaaacatcat aaatgaactt gaacgttttg
    67561 aaaaagaaaa gcaaatctca acttttgaga cacgtgaact gcagcagttt atcgaagatt
    67621 acgaaaagca aacgaacgaa ttgaaagaca aacaaacaga tcttgaatcg caacgacaaa
    67681 aaatcgacga agaaatcaaa tcgttaagtc aagaaagcga ccagatggaa gccagaagag
    67741 cacaagtaca aagtcaaaaa gcacaagaac aagcagatct tgctgtcttg aaagagcagt
    67801 ttaatcatct gcagatccag ctccgcggtg cccgagtcca aaaagcagag gcaaccgaac
    67861 gtcaagaagc gatagaaaag cagttggcga cattgacagc agacttttct gatcatgaag
    67921 tcacagaaga aagtttggaa aaacaaatta acgaattatc tgctcagcga gaaacattaa
    67981 aagccgagtt ggcaaaagcc aaagagcagc gagatagaag gcaaaaggag atcgataagc
    68041 ttgaagcagt cttggcagag cgtaatcgtg agcaaaaagc acgactgtcg gaacaatcca
    68101 aattagaagt ccaaaaagac cgagctgaaa tgattttaga taatcatttg acctatctgc
    68161 agtcagaata tcagctgaca tttgagaaag cttcccaaga ctatcaagaa acaaccgata
    68221 tcgaagacag tagaacaaag gtttcatcct taaaagaaca gattgaaaaa ctgggaccag
    68281 taaacttaaa ttcaatcgaa caatacgaac aagtcagtga aagacataca ttcttagcaa
    68341 cacagcgaga tgatctgtta gcagcgaaaa accaattgtt tgaaacgatg gatgagatgg
    68401 atgatgaagt gcggacaaga ttcaaagaag tatttgaagc tatccgtcaa gaattcaaaa
    68461 ttgtctttcc aaatatgttt ggcggcggta aagctgaatt agtactgacg gaaccaactg
    68521 atttgctgaa aacaggtatt gagattgaag tacaacctcc tggcaaaaaa ctgcaaagtc
    68581 taagtttgct ttctggagga gaacgagcac ttacagccat cgcgttgctc ttttccatta
    68641 tccgtgtctg tcctgttccg ttttgtatct tagatgaggt agaagcagcg ttagatgaag
    68701 ccaacgtgaa acgttttggt cgttacttga gcgatttcca agatgacaca caatttatcg
    68761 tagtcacaca ccgcaaagga acgatggtcg cagcagatgt actgtacgga gtgacgatgc
    68821 aggaatccgg tgtatcaaaa atcgtttctg tacggatgga agatatcaat gaagaggcga
    68881 aaatcaacga aacgaaagtg taggaacgaa aatgatcaaa ttagtagcaa tcgatttgga
    68941 cggtacatta ttaaatgaac aaaagctgat ttcagatgag aataaacaag cattagcaca
    69001 agcaaaacag caaggggtca agatcgttct atgtacagga cgccccttag ctgcgatggc
    69061 gcattatctg caagaattag gactggtaga tgaaggtgac tacagcatca ctttcaacgg
    69121 cggtttggta caaaaaaatg atactggtga aatcatggaa aagaaagtga tggaagtatc
    69181 agatatccaa cgcttataca aactggctca gcaattggat ctgccactag atgttttgtc
    69241 tgacaatgtg gttttacagc tgcctagtgc cccaaggaag caatccttgt acaatgtgtt
    69301 gaataagctg ctgcaattcc aaccagcgga tataagcgat attacagatg aatttgtgct
    69361 taataaggca gtaatcgctt atgaccaaga agaattagat cctaaaatca aagaaatacc
    69421 agcagaattc catgaaatgt atgagatcat caaaacaaga agtatgcttt tggaatttat
    69481 gccaaaaggt gttacaaaag cttatggtat ttctttattg gcaaaagatc ttggtttgaa
    69541 acaagaagaa atcatggcga tcggtgatga ggaaaatgat ttaccgatga tccaatatgc
    69601 tggactaggc gtagcgatgg agaatgctgt gcctttcgtc aaagaagcag cagatttcgt
    69661 gacagccagc aatgaagaaa atggcgtagc caaagtgatc caaaaatttg tattagagta
    69721 gggggagtca aatgggattt tttgataaaa tcaagcgtgc ttttactggc gaagagaaaa
    69781 cagaagaagt tgaagtacag gagaagtacg acaagggttt agaaaagacg cgtaaaacat
    69841 ttggtgaacg gatgaatgag ttgtttgcca attttcgaac agtcgatgaa gacttttttg
    69901 aagaattaga agaaacactt atcggtgcag atgtaggttt tgaaacagcg atacgaatca
    69961 ccgaagcatt acgtcaagaa gtgaaactca gaaatgcgaa aaaatcggca gaagtccaaa
    70021 atgcgatcat cgagaaactt gtggatctct atgaagcgga aggaatcaat gaagtaaatg
    70081 aactaaatat ccaagaagac ggactaacag ttattttgtt tgtcggtgtt aatggtgtag
    70141 ggaaaacaac aagtattggt aaacttgccc atcagttcaa aaacgaaggt aaaaaagtat
    70201 tgatggcagc agcagatacc ttccgcgctg gagcaatcga ccagttagtt gtctggggcg
    70261 aacgcgctgg tgttgaagtt gttcgtggca atgcaggagg cgatccagca gctgtcgtat
    70321 tcgatgcttt ggaaagagca aagacagagc aggcagatgt tttactagtt gatactgcag
    70381 gccgactgca aaataaagtg aacttgatga acgaattaga aaaaataaaa cgagtaatcc
    70441 agcgggaatt accagaagca ccccaagaaa cacttcttgt attagatgca accactggtc
    70501 aaaacgcgat ggttcaagca aaacaattca aagaaacaac agatgtgacc ggtcttgttt
    70561 tgacaaaatt agatggtaca gcaaagggcg gtatcgtact tgctatccgt aatgaattgc
    70621 atttaccagt gaaattggtc ggtcttggtg aaggaatcga tgacttggag ccgtttgatc
    70681 caaatgattt tgtcgttgga ttgttcaaag gattactaaa agaagaataa cccatgtcta
    70741 aaaagcagga gctgtgacaa aagttgcagc tccttgtttg aatgattgtt tttaacatag
    70801 ctgtgttttt gatgccttca tttttatact tatttgtaag cggttctcaa acaatttcgt
    70861 tctttttctt agactttttg attataatta gattcatatg aattggaggt cacttaatga
    70921 tgaattttaa gcaattatta ttgcatgtca atgcgcgccc ttttatcgac caagctcgat
    70981 ttggtataga acgggaagga cagcgggttg atcttgcagg aaatctagca aaaaccgatc
    71041 atccagcaat ctttggcgat cgatcctatc acccctatat ccaaacagat tttagcgaaa
    71101 cacaaacaga gatgatcacc cctgttaccg attctattcc cgaattattt cagtatctcg
    71161 ctgctgttta tgatgtgact gctcgttcta taccgaaaga agagatgatc tggccattaa
    71221 gtatgccacc tgccttaccg gaaaaagacg aagagatcat tattgcaaaa ttaaaaaatt
    71281 tcgaagatgt cttgtatcga cgttatttag caaaagaata cgggaagcga aaacagatgg
    71341 taagcggtat tcatttcaat tttgaatttg gcgatgagtt gctaagaaca ttgttcagcc
    71401 atcaggaaga atttcaagat ttttccgaat tcaaaacaga actttatttg aaaacagcga
    71461 gaaactttat gcgctatcgc tggatgatca cttatttatt cggtgcttca ccaatgagtg
    71521 agaagaacta ctttttagat gaatcacatc cgcaagaacc tgttcgcagt attcgcaaca
    71581 gtgcgctggg ctatacgaat catccaaatg tgaaagtttc ctatgcttct atgaaacagt
    71641 atttagcaga tatcgagcga atgatcgaag aagggaaact ttcggaggag aaagaatttt
    71701 atacaccgct tcgtttccga ggcggaaaga aagtcgcaga tttagcaaca acaggtgttc
    71761 gttatattga attgcggaat atcgatttga atccttatgc aagattgggt atcaatccag
    71821 agcaagttcg gttcttacaa ttattcctga tgtacatgtt atggacagaa gaaaaagaag
    71881 actgtgacca atgggtagca gaaggtacga ctcgaaataa caaagtagca ctagaacaac
    71941 catcagatca aacagaattt catcaagaag gtagagaaat tcttgaagga atgaagcaaa
    72001 tgctggttga attggattgg ttggattctc tttatttagt agaggaagca ttgactcaaa
    72061 tggatcatcc tgaacaaacg ttagcagcga aactctatca agaagcacag ctatcgagtc
    72121 agcaagaagt tgctgtggca ttgggacatc aatattataa agaaagccat gaacgtccct
    72181 atcagttggc cggttttcgc gaaatggaac tttctactca gatatttatg ttcgatgcca
    72241 tccaaaaagg tgtccaagtc aaagtattag atgagtcgga tcaatttttg cgtctgcaat
    72301 tccaagacca tgtagaatat gtgaaaaatg caaacatgac aagtaaggac agctatatcg
    72361 tcccacttat catggaaaac aaaacagtaa cgaaaaaagt cttaaaagaa gctgggttcc
    72421 gggtaccagg aggcgcagaa ttttcatcca tggaagaagc ggtaaaagct tatccgagat
    72481 ttgccgagca ggcatttgtg atcaaaccta aatcaaccaa ttacgggtta ggtatcacga
    72541 ttttcaagga aggagctagt ctggaagatt atcaagcagg tttagccatt gcttttcgtg
    72601 aagacagttc ggttttagta gaagaattta tgccgggaac ggaatatcgt ttctttgtga
    72661 tcgacggaga agtccaagcc atcatgttgc gagtccctgc taatgttatt ggtgacagta
    72721 tccgaacagt gaaagaactt gttgaagaaa aaaatagtga ccctttgcgt ggaacgaatc
    72781 atcgtgcacc tttagaattg atccaattag gtgaattaga acaattgatg ttgaaagaac
    72841 aagggttgac gatcgaatca gttccccaag ccaatcaaat tgtttatcta agggagaact
    72901 caaatatcag tacaggtggt gattcgatcg atatgactga tgaattttct gaagcttaca
    72961 aaaaaatcgc cgtttctgcg gtagaagcac taggagctaa gattagtggt atcgatttga
    73021 ttataccaga taaagaaatc gatcctacaa ctgataaaaa agcatatggg atcatcgaag
    73081 caaatttcaa tccagccatg catatgcatg tttatccttt tgcaggaaaa ggcagaagat
    73141 tgacgatgaa tgtattgaaa ttgttatatc ctgaagtatt ttaaaagaaa aagcttttga
    73201 cagattgtcc tatttgatgt caatctgatc aaaagctttt tttgcatgta aaaatttatg
    73261 tgttttttcg ttttaaaata aacgatccca gaagcttttt ttcttttttg gtttttcttt
    73321 ttcagctggc gaagagtttt cttctgggta ttttttttcg atatcaatca caggttcatt
    73381 tactgcttct tttgctgtta agagcaatcc gaattcctca ggatttgcag tgacgtaatc
    73441 gttgactaca gtgaaacggc tattgttttt cgttgctagc tggatatatt ggttttggac
    73501 agaaaggggt agagccccat tcaagagtat ggaagcatct ggatgttttt tgagttcttt
    73561 taaaaagttt tctttgtttt ttggttggat catttcagca acagtcatac taagatagca
    73621 gcgttccctg aatgtcccta aatatttatg ctgttcttca gggttcacta aaggtgtacc
    73681 ataaagtccc ttatccaaat ggctttttac atcatcttgt gccataaaaa ataccctcct
    73741 tcttctcttt gtctttctta cgattatagc atattcccaa taatacctaa aacatatgaa
    73801 aaagcggttg cttttgttca acgattcact taaaataaga atgaaaatca ttctcaatta
    73861 gaaaggtgga ttggatgcgc tacggacgac tagtagcatt attagttatt ttaagtagta
    73921 tttcattgtt catcggagta tcgaatgtac aattgtcaga actgctccag ttagatgggc
    73981 aaagcttaca aatcttgcta gtgactcgtt tgccacgaac gatttgtttg atccttgtgg
    74041 gagcgaccag tagtatttgt gggttgatca tgcaacattt gacccaaaat aaatttgttt
    74101 caccaacgac tgcgggcacg atggatagtg cgcgtttagg tattttagtg gctatgattt
    74161 tcctacctgg tgcatccttg attgtccgat ccttgacagc attttgcttt gcttttgcag
    74221 gaacattgct ttttttatcc cttacgcgat tgttcctcca gaaaaatcaa ttgatgcttc
    74281 cgttgattgg agtcatgtta gggaatatca ttggttcagt tgctactttt tttgcttatc
    74341 aattccagtt ggtacaaaat atgacttcat ggcttcaagg gaattttgcg actgtgatac
    74401 gaggaaatta tgagttattg tatgctgttg ttccattgtt gctcttggtt tggctatttg
    74461 cttaccgatt tacgatcatt ggtctgggcg aatcgtttgc agtaaatctg ggaatagatt
    74521 accagaagat gcagttttta ggcattggct tagtatcatt ggctagtgca gtcatgttga
    74581 tcatggtagg aagcattcct tttatcggag tgatcattcc gaatcttgtc tctagaatat
    74641 atggagatca agtgcacaaa acaattggcg taactgcttt agcaggtagt ttgttcttag
    74701 tattttgcga tcttgtcgct cgcgtgttga tttttcctta tgaaatacca gtcagtgtga
    74761 tcgttggaat catcggtggg gctatcttcc tttatcttct gataagggga cgacgatatg
    74821 cttaaaaaaa agccatctat ttttattggt atgcttgttc ttgttttatt tgttacagga
    74881 atgtacttga cgatccatac atatggaaac tggcattttg catgtgcatt gaggagtaag
    74941 aaaatttttg catttatcct tgtctcgcta gctgtcagca gtgccacaat cagctttcag
    75001 acaattacgc ataaccagtt tttgactcct tcagtattgg gacttgagtc gttatatgtc
    75061 cttgtccaaa cggtcctttt cttctttatc ggaggagtga cgatgcttca gcagacaggt
    75121 acagtttttt tcttattgaa cgttgggctg atggtcgggc tgagtctatt gttatttttc
    75181 tttcttttga aaaaaatgag agcgaatctt tttttattac tgatgtcagg gatgatttta
    75241 ggtacgcttt ttggtagtgt cagtaccttt ttacaagtca tcatggatcc gaatgaatat
    75301 gatttgcttc aaggcagatt atttgcaagc tttggcaata tccagagtca gtatcttttg
    75361 cctgcttctt tgatcattat cggctgctgg tacctgttat ttcgatatcg acatgtgcta
    75421 gacgttttcc atctcggaaa tgaacaagca gtaaacctcg ggatagacac agatcgtttt
    75481 gaatttttga ttttatgttg tgtcagtctt ttgacaggga catcaacggc tcttgtgggt
    75541 ccagttactt ttttagggtt tattgtcgca aatatcagtt atcgtgtctt ttctacttat
    75601 cgtcatggtc agctatttat cggaagcttt ctggtagcta tgctgtttct gacaggagga
    75661 cagctcgcag tagagcagat tttcggctgg aatacaacag tgagtgtcgt cgttgaattt
    75721 gtcggaggaa tttattttgt cacaaaattg attcgcgaaa ggaagagtat cctatgattg
    75781 atttacaaaa ggtcaataaa ctgtaccatg gaaaatcagt ggttaaagat gtacagttaa
    75841 cgattcctga aaagaagttg actgctttta tcggtccaaa tggtgctgga aagtcaacgg
    75901 tgttgtcgat gatcagtcgt ttgataccaa aagatacagg tgaaatctat ttagaccata
    75961 acgaagtaaa ggcttggaaa tccaatgaat tagccaaaaa attatcgatc ctacgacaat
    76021 cgaatgaaat aaatgtccaa atcaccgtga aagaattagt agaatttgga cgatttcctt
    76081 atacgaaagg aaaattgacg acaaaagacg aggaaatcgt tcaggaatca ctggaacaca
    76141 ttgggttaga agaaatagcg gatcaatcga ttgacactct ttcaggcggt caactccaac
    76201 gtgcgtatat tgcaatggtg ctagcgcaag acacggatta tattttactg gatgaacctt
    76261 tgaacaatct agatatgaat tttgcggttc aaatcatgca gattttaaaa aatttagtac
    76321 aagaaatcgg gaaaacaatt gttattgttc ttcatgatat caactttgct gcaagttatg
    76381 cagacgaaat tgttgcaatg aaagatggac agttatttac gcaaggaacg acccaagaaa
    76441 tgataaaaaa agatgtattg gatgaattat acgaaatgga catacggatt tgtgagattg
    76501 aaggccgcag attctgtctt tatttctaaa actaacatta ggagagaaga gatgaaaaaa
    76561 ttagtagtag gtatcttgat tctagcatca tttggtttag cagcatgtgg aaatagcaac
    76621 aacaccagtc aggcagacac aaaaaaaagc agcacacaaa ctgaaactac attgacgata
    76681 acagatagta atggagacca aatcgaggtg ccaaataatc cagaaaaagt cgtcgtattc
    76741 gacaatggtt ctttagatac gatggatgct cttggtgttg gagataaggt agttggagca
    76801 gctacaagca gccttcctga gtatctttca tcctataaaa aagtagaatc agcaggtgga
    76861 ataaaagaac cagacttaga aaaaattaat cagcttcagc cagatctaat catcatttct
    76921 ggccgacaaa gagattttca aaaagatcta agtcaaattg ctccgaccat tttcctttct
    76981 ttagatgcga aaaatccatg ggaatctttc cagcaaaatg tcactgcttt aggggagatc
    77041 tttggtaagc aagaagaagc taaaactcaa ttagaggaat tatctagtgc aatcgatcag
    77101 acgaaaaaga aagcagaagc tactgacaaa aaagcactag tcacacttgt gaatgaaggg
    77161 caattatcag cctacggatc tggttctaga ttcggtttca tccatgattt atttggcttt
    77221 gaacaagcag atgatcaaat cgaagcatcc actcacggtc aaagtgtttc ttatgaatat
    77281 gtgctggaaa aaaatccgga tatcttattt gtagtagaca gaacaaaagc tattggtgga
    77341 gacgacagca aagatgatat ttctgccaat gaactggtag cacaaacgaa tgctggtaaa
    77401 aatcagcaga tcatctccct tgaaccagat gtctggtatt taagcggggg cggtttagaa
    77461 tcaatgaaac tgatgatcga agatgtgaat caagcattta aatagcataa ataacagagg
    77521 attaaactct catactatgt aacaataatg tttcttacat tcttaaatag caagagccta
    77581 tcctcaaaga agttatcaaa aaccatccgt aaaatcgtat tgatgatttt acggatggtt
    77641 tttatttatt tgagaaatct tttttcaacg tgaattcatt atctacatca tacgggttga
    77701 ttctagaatg gggagtggca aaaaatagtg tgatccattt tttcagcatc aagatcgcct
    77761 cctttgattt gaatcaaagg tacaaagcga aacttagata aaacttaaat ttagatattt
    77821 ttattttgta ccgatctctt ctttaaacga tgttccatct gctgcttctc ggcaataagt
    77881 ccactagact agaaaatatg agaagctatt ttctgtccag cgttcttatt actcaaaaca
    77941 aatcgcagtt gtcacaatct cttctaaacg atgttccatc tgctgcttct cggcaataag
    78001 cccactagac tagaaaatat gagaagctat tttctgtcca gcgttcttat tactcaaagc
    78061 aaatcgcagt tgtcacaatc tcttctaaac aatgttcaat cagctgacct tcggtattag
    78121 ctcaaagcac tgatttcaca acctctattt aggcatgtgt ctcagcaagc ggttgatagt
    78181 tgatgatctg catttttcga taaaaatcgg cgggaagctt actccaccgt tttagtaatt
    78241 gatagaaata actctcttct gctttgattt tatactcgct taatatcaat ccaaaactat
    78301 tcgttttttt tgaagctaat tttttataaa tatccaatgc tttggtttta tcttcattga
    78361 caaagagtgt ttttacttca atataagaac cctcgatatt ttgtgtttct tcgataaaat
    78421 agatgttttt tatatattcc ttcatagtga ttcctccaaa gaagtctttt atttgataag
    78481 tttactctac cgaagagttg ttaataatcc gtgaacaaaa agtaattctt tttccaatag
    78541 ttgttaaagg ttttttattt tttatagtat tttttgttat ttaagaaagg aataaggtta
    78601 aacaaataac aggttgcttt ttgggtcttt ttccgttcaa gaaactgaac gaagaaaagg
    78661 gaaagttgtc tcttttgtga taagagaaat cataataaac gaatgactta ctttccaata
    78721 aattgagaac agtcagaacc agacaaatta gttgcatctg atgaataaag tgattattct
    78781 atgacatgag gtgatacaga tgaaattaga agtgacaaaa gaagcacaag aagtattgaa
    78841 gaataaatta gaagcagacg atcaactttt attagatatc gaaaacggtg acggtccatt
    78901 tgcagaaagc caagtcagtt gccaattgga tacggcattt cgattgatca ttgtcaaaaa
    78961 agatacacaa acagatctaa gtctttattc tgtagtggct gatacaccag ttggaccaat
    79021 caaaatcaaa gacagtgcaa tactttacat ggatgatcca tccacacttg ctttagaacc
    79081 aacttataat agtttacaat taaaaggacc aagcggcttg agaaaaggca acttgcaagt
    79141 tgttcgaaaa gactagaggg ataaaatggg aaaattggcg atcatcagcg acctacatgt
    79201 ggacatcaat aaattcggcg aatcagagct gatgcagctt tggcaagttt tgcaaaagca
    79261 caagatcact cggcttcatc tagccggaga tacggctaat aaagtagacc gttgcctagc
    79321 tgttgtcgaa tttttcgtca ataaaggatt atctacgaca tttcatttcg gcaatcacga
    79381 gttggcagat gtgcccaatg aacagatgat ggaccatttt ccagacccac attttttaaa
    79441 tgaacggtat attgctttaa atgagcagac cgttctttta gggatgaatg gctggtacga
    79501 ctatcagttt tccgaattga aagattacga ggcaatcgta cgaatgaagc gtttgtattg
    79561 gtatgatcgg ttcatccagc gtgggaaaac agatctcgag gtcaatgcag aaatgcttgc
    79621 agctgcaaaa aatttgctag accagctgga agcaaaaaaa cttgaggtga ttttatccac
    79681 tcatttcgtt cctaaacggg catttatcat gtatcaatct gctccttatg agcgttggaa
    79741 caaattaaat gcatttttag gatcagaatc attcggagaa ttacttgacc gttattcaaa
    79801 tatcaaacaa gtagtttttg gccatacgca tcgtcgcttt gaagataaat ccatcggtca
    79861 cacgatttac agctgcagac cttttggtta ttattatgaa tggcagctga cccgagattt
    79921 tgtcttgaaa gaacagttgc tagacagcta tgatcctatg aaattacgtg tcctcttgcg
    79981 tgcccatcac ccagcttttc aggcctatca aacacaacat ttgcaagagg aatttgaaca
    80041 agcgatgaca atcgttccgt attgagacag cattagtaat gtgtaaaaga aaataccttg
    80101 taaaacataa ggataagtaa gagcagtaga tccatttatc gtggatatgc tgcttttttt
    80161 gcggaaaata atcagcgaat gtttatttga tcggatgatt tgtaactcgc agagtactag
    80221 atgcaagcgt gtattctttt tttgaacgat aatcacaaaa aaaatataat atacattttt
    80281 tatttaatta taattaatat tgattgaagt ctaggcagtc aagtcgagag t
//
LOCUS       DAFGZE010000003        76963 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.003, whole genome shotgun sequence.
ACCESSION   DAFGZE010000003 DAFGZE010000000
VERSION     DAFGZE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 76963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.003"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(293..772)
                     /gene="rlmH"
                     /locus_tag="LTU54_000172"
     CDS             complement(293..772)
                     /gene="rlmH"
                     /locus_tag="LTU54_000172"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBL8294915.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            1200..2489
                     /locus_tag="LTU54_000173"
     CDS             1200..2489
                     /locus_tag="LTU54_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBL8294916.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2528..3133)
                     /locus_tag="LTU54_000174"
     CDS             complement(2528..3133)
                     /locus_tag="LTU54_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HBL8294917.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3338..3802)
                     /locus_tag="LTU54_000175"
     CDS             complement(3338..3802)
                     /locus_tag="LTU54_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL8294918.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3824..4144)
                     /locus_tag="LTU54_000176"
     CDS             complement(3824..4144)
                     /locus_tag="LTU54_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL8294919.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4288..5367)
                     /gene="pepA"
                     /locus_tag="LTU54_000177"
     CDS             complement(4288..5367)
                     /gene="pepA"
                     /locus_tag="LTU54_000177"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HBL8294920.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5567..5917
                     /locus_tag="LTU54_000178"
     CDS             5567..5917
                     /locus_tag="LTU54_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL8294921.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            6140..7087
                     /locus_tag="LTU54_000179"
     CDS             6140..7087
                     /locus_tag="LTU54_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL8294922.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(7144..8562)
                     /gene="pepV"
                     /locus_tag="LTU54_000180"
     CDS             complement(7144..8562)
                     /gene="pepV"
                     /locus_tag="LTU54_000180"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HBL8294923.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            8711..9551
                     /locus_tag="LTU54_000181"
                     /pseudo
     CDS             8711..9551
                     /locus_tag="LTU54_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
     gene            9555..9995
                     /locus_tag="LTU54_000182"
     CDS             9555..9995
                     /locus_tag="LTU54_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HBL8294924.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            10045..10239
                     /locus_tag="LTU54_000183"
     CDS             10045..10239
                     /locus_tag="LTU54_000183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294925.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            10375..11134
                     /locus_tag="LTU54_000184"
                     /pseudo
     CDS             10375..11134
                     /locus_tag="LTU54_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(11542..12231)
                     /locus_tag="LTU54_000185"
     CDS             complement(11542..12231)
                     /locus_tag="LTU54_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294926.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(12206..14335)
                     /locus_tag="LTU54_000186"
     CDS             complement(12206..14335)
                     /locus_tag="LTU54_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBL8294927.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(14383..15720)
                     /locus_tag="LTU54_000187"
     CDS             complement(14383..15720)
                     /locus_tag="LTU54_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HBL8294928.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(15972..16331)
                     /gene="rplT"
                     /locus_tag="LTU54_000188"
     CDS             complement(15972..16331)
                     /gene="rplT"
                     /locus_tag="LTU54_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBL8294929.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(16384..16584)
                     /gene="rpmI"
                     /locus_tag="LTU54_000189"
     CDS             complement(16384..16584)
                     /gene="rpmI"
                     /locus_tag="LTU54_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBL8294930.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(16656..17180)
                     /gene="infC"
                     /locus_tag="LTU54_000190"
     CDS             complement(16656..17180)
                     /gene="infC"
                     /locus_tag="LTU54_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBL8294931.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(17224..17349)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            17500..18132
                     /locus_tag="LTU54_000191"
     CDS             17500..18132
                     /locus_tag="LTU54_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL8294932.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(18134..19039)
                     /locus_tag="LTU54_000192"
     CDS             complement(18134..19039)
                     /locus_tag="LTU54_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL8294933.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(19124..19783)
                     /locus_tag="LTU54_000193"
     CDS             complement(19124..19783)
                     /locus_tag="LTU54_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL8294934.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            20099..21043
                     /gene="mvk"
                     /locus_tag="LTU54_000194"
     CDS             20099..21043
                     /gene="mvk"
                     /locus_tag="LTU54_000194"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HBL8294935.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            21036..22013
                     /gene="mvaD"
                     /locus_tag="LTU54_000195"
     CDS             21036..22013
                     /gene="mvaD"
                     /locus_tag="LTU54_000195"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HBL8294936.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNFKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            22026..23111
                     /locus_tag="LTU54_000196"
     CDS             22026..23111
                     /locus_tag="LTU54_000196"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HBL8294937.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            23108..24172
                     /locus_tag="LTU54_000197"
     CDS             23108..24172
                     /locus_tag="LTU54_000197"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBL8294938.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(24421..25224)
                     /locus_tag="LTU54_000198"
     CDS             complement(24421..25224)
                     /locus_tag="LTU54_000198"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBL8294939.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(25244..26086)
                     /gene="kduI"
                     /locus_tag="LTU54_000199"
     CDS             complement(25244..26086)
                     /gene="kduI"
                     /locus_tag="LTU54_000199"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBL8294940.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(26102..27109)
                     /locus_tag="LTU54_000200"
     CDS             complement(26102..27109)
                     /locus_tag="LTU54_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBL8294941.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(27116..27760)
                     /locus_tag="LTU54_000201"
     CDS             complement(27116..27760)
                     /locus_tag="LTU54_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBL8294942.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(27860..28618)
                     /locus_tag="LTU54_000202"
     CDS             complement(27860..28618)
                     /locus_tag="LTU54_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBL8294943.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(28745..29896)
                     /gene="fucO"
                     /locus_tag="LTU54_000203"
     CDS             complement(28745..29896)
                     /gene="fucO"
                     /locus_tag="LTU54_000203"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBL8294944.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(29978..30289)
                     /gene="rhaM"
                     /locus_tag="LTU54_000204"
     CDS             complement(29978..30289)
                     /gene="rhaM"
                     /locus_tag="LTU54_000204"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBL8294945.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(30291..31136)
                     /gene="rhaD"
                     /locus_tag="LTU54_000205"
     CDS             complement(30291..31136)
                     /gene="rhaD"
                     /locus_tag="LTU54_000205"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBL8294946.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(31150..32406)
                     /gene="rhaA"
                     /locus_tag="LTU54_000206"
     CDS             complement(31150..32406)
                     /gene="rhaA"
                     /locus_tag="LTU54_000206"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBL8294947.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(32403..33863)
                     /gene="rhaB"
                     /locus_tag="LTU54_000207"
     CDS             complement(32403..33863)
                     /gene="rhaB"
                     /locus_tag="LTU54_000207"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBL8294948.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            34016..34885
                     /locus_tag="LTU54_000208"
     CDS             34016..34885
                     /locus_tag="LTU54_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBL8294949.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(35011..35910)
                     /locus_tag="LTU54_000209"
     CDS             complement(35011..35910)
                     /locus_tag="LTU54_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8294950.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(36075..36899)
                     /locus_tag="LTU54_000210"
     CDS             complement(36075..36899)
                     /locus_tag="LTU54_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL8294951.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(36910..38205)
                     /locus_tag="LTU54_000211"
     CDS             complement(36910..38205)
                     /locus_tag="LTU54_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBL8294952.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(38221..38700)
                     /locus_tag="LTU54_000212"
     CDS             complement(38221..38700)
                     /locus_tag="LTU54_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBL8294953.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(38706..39734)
                     /locus_tag="LTU54_000213"
     CDS             complement(38706..39734)
                     /locus_tag="LTU54_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBL8294954.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(39751..40857)
                     /locus_tag="LTU54_000214"
     CDS             complement(39751..40857)
                     /locus_tag="LTU54_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL8294955.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(41086..43683)
                     /gene="adhE"
                     /locus_tag="LTU54_000215"
                     /gene_synonym="adhC"
     CDS             complement(41086..43683)
                     /gene="adhE"
                     /locus_tag="LTU54_000215"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBL8294956.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(43988..44368)
                     /gene="yajC"
                     /locus_tag="LTU54_000216"
     CDS             complement(43988..44368)
                     /gene="yajC"
                     /locus_tag="LTU54_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBL8294957.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(44465..45610)
                     /gene="tgt"
                     /locus_tag="LTU54_000217"
     CDS             complement(44465..45610)
                     /gene="tgt"
                     /locus_tag="LTU54_000217"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBL8294958.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            45828..46526
                     /locus_tag="LTU54_000218"
     CDS             45828..46526
                     /locus_tag="LTU54_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL8294959.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(46694..49333)
                     /locus_tag="LTU54_000219"
     CDS             complement(46694..49333)
                     /locus_tag="LTU54_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL8294960.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(49952..50983)
                     /gene="queA"
                     /locus_tag="LTU54_000220"
     CDS             complement(49952..50983)
                     /gene="queA"
                     /locus_tag="LTU54_000220"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBL8294961.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            51176..52351
                     /locus_tag="LTU54_000221"
     CDS             51176..52351
                     /locus_tag="LTU54_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL8294962.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            52355..52996
                     /locus_tag="LTU54_000222"
     CDS             52355..52996
                     /locus_tag="LTU54_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294963.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            52993..53910
                     /locus_tag="LTU54_000223"
     CDS             52993..53910
                     /locus_tag="LTU54_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8294964.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            53907..54578
                     /locus_tag="LTU54_000224"
     CDS             53907..54578
                     /locus_tag="LTU54_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8294965.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(55119..55607)
                     /locus_tag="LTU54_000225"
     CDS             complement(55119..55607)
                     /locus_tag="LTU54_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HBL8294966.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(55808..56074)
                     /locus_tag="LTU54_000226"
     CDS             complement(55808..56074)
                     /locus_tag="LTU54_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL8294967.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(56074..56337)
                     /locus_tag="LTU54_000227"
     CDS             complement(56074..56337)
                     /locus_tag="LTU54_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL8294968.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            56813..56998
                     /locus_tag="LTU54_000228"
     CDS             56813..56998
                     /locus_tag="LTU54_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8294969.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(57140..58963)
                     /locus_tag="LTU54_000229"
     CDS             complement(57140..58963)
                     /locus_tag="LTU54_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HBL8294970.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(59561..62266)
                     /locus_tag="LTU54_000230"
     CDS             complement(59561..62266)
                     /locus_tag="LTU54_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBL8294971.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(62572..63519)
                     /locus_tag="LTU54_000231"
     CDS             complement(62572..63519)
                     /locus_tag="LTU54_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HBL8294972.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            63709..63978
                     /gene="rpsN"
                     /locus_tag="LTU54_000232"
     CDS             63709..63978
                     /gene="rpsN"
                     /locus_tag="LTU54_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL8294973.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(64154..64516)
                     /locus_tag="LTU54_000233"
     CDS             complement(64154..64516)
                     /locus_tag="LTU54_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL8294974.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(64527..65648)
                     /locus_tag="LTU54_000234"
     CDS             complement(64527..65648)
                     /locus_tag="LTU54_000234"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBL8294975.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(65664..66014)
                     /locus_tag="LTU54_000235"
     CDS             complement(65664..66014)
                     /locus_tag="LTU54_000235"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBL8294976.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(66144..67655)
                     /locus_tag="LTU54_000236"
     CDS             complement(66144..67655)
                     /locus_tag="LTU54_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL8294977.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(67977..69329)
                     /locus_tag="LTU54_000237"
     CDS             complement(67977..69329)
                     /locus_tag="LTU54_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBL8294978.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(69482..70558)
                     /locus_tag="LTU54_000238"
     CDS             complement(69482..70558)
                     /locus_tag="LTU54_000238"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBL8294979.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            70754..72499
                     /locus_tag="LTU54_000239"
     CDS             70754..72499
                     /locus_tag="LTU54_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBL8294980.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(72779..73216)
                     /locus_tag="LTU54_000240"
     CDS             complement(72779..73216)
                     /locus_tag="LTU54_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBL8294981.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(73200..74573)
                     /locus_tag="LTU54_000241"
     CDS             complement(73200..74573)
                     /locus_tag="LTU54_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8294982.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(74895..75383)
                     /locus_tag="LTU54_000242"
     CDS             complement(74895..75383)
                     /locus_tag="LTU54_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8294983.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(75405..76007)
                     /locus_tag="LTU54_000243"
     CDS             complement(75405..76007)
                     /locus_tag="LTU54_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL8294984.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(76076..>76963)
                     /locus_tag="LTU54_000244"
     CDS             complement(76076..>76963)
                     /locus_tag="LTU54_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HBL8294985.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT        22058 a        16411 c        14037 g        24457 t
ORIGIN      
        1 tatttgtttt cccagtccat ttagatagtt ttcttcaata tcatccatat cctctaatag
       61 attttttaaa cacttttttt catctatcat gttttttctc cttgccatta ttctaactaa
      121 aattttacgt atttgtttct atgtctacat tatcttttta acagtatcta tttttctttt
      181 gcaacttact tggctaccaa agcaaataaa aaagttattt ataaatttct ccctaccact
      241 acctattgct attatttaaa atagggataa ttttttctct taaaaaatat cattatttgt
      301 ggtatggagc ccccgcattg atccggaagg cacggtagat ttgctcgaca aggatcagac
      361 gcatcaattg atgtggatac gtcatttttc caaaagagat ctgagcattg cttcttttca
      421 tgacggcttc gctgagtcct aaagagccgc cgataacaaa aacaaggtgg ctttttcctt
      481 ggatacctaa ttggtcgatt tgtttagcaa aagattcact tgtaggattt ttcccttcaa
      541 ttgctaaggc atagacgtat tcttgttctt tgattttcgc taagatccgt tccccttctt
      601 tttcttttac ttgtctcatt tgcgcttcgc ttaaattctc cggggctttt tcgtcaggaa
      661 cttcgatcaa ttcgattttt gcataggcat tcagccgttt gacatactca ttgatacctt
      721 ggatcaggta tttttctttt aattttccta ctgagataat tttgatattc atgttttctc
      781 tcctttggtc ttcatgctca ctttgtttat tataatcaat tttttcgtct atatgtttat
      841 tttggctatt tttgttaaaa agatttcccc aacagttatg cacaacatcc acaggtttat
      901 ccacagaaaa cagtgttatt ttaccttatt tcatcacatt cattaggcaa acatacgttt
      961 tataagaatt tatccacttt atacacaggc tgtggatatg ttttatccct tatttttcca
     1021 atcctatccg cgtagtttta cacagtatct gtggaaaaag aaaatcaaat attttcatct
     1081 gaaaacaatg atttttccta aagtttttca taaaaagttc acagtctcaa agtagactaa
     1141 agtcaacaaa taagactata ctaacagtag ataaatgaat gtaattggag gaggaacaca
     1201 tggatagaaa aaatgtgaca ccaaagatga aaaaaagtaa aaataatagc ctctggcgta
     1261 agttaggtct ggggcttgtt ggagggatcg ttggaggact actgacagct ggcatttttt
     1321 atgcagcgat gggcactggc aacaatagtt ctactgctac aagcggcaat cagaattctg
     1381 ccggtgaaac agttgtggaa aatgtcaaag tcaatgtaga ttctgatatc accaaagcgg
     1441 tagataaagt tcaagacgct gtcgtttctg ttatcaactt acaaagtcaa aaccaatcaa
     1501 gcggttttgg cggactattc gggcagcaag aagaaagtag cagctcagat gatagtaatt
     1561 tggaagctta tagtgaagga agcggcgtga tctacaaaaa agacgggaat actgcttatg
     1621 tagtcacgaa taatcacgta gtagatggcc agcaaggtct tgaagtcttg atgaaagacg
     1681 gtacgaaggt aaaagctgaa ttagttggaa cagatgctta ttcagattta gcggtgttga
     1741 agatcaattc agacaaagtc gaaacagttg cctcttttgg tgactcaagt gctctgaagg
     1801 ttggtgagcc tgcgattgcg attggttccc cactaggatc tgaatatgca aattctgtca
     1861 cttcaggaat catttcttcc ttgaaccgac aagtgacaag tacgaatgaa tcgaacgaaa
     1921 cagtcaatat caatgcgatc caaacagatg cagcaatcaa ccctggaaat tctggtggtc
     1981 ctttagttaa tatcgaagga caagttatcg gtatcaactc aagtaagatc gcaagtactt
     2041 ctgaatctac ttctaatgta agcgtagaag gaatgggatt tgctattcca agtaacgatg
     2101 tagtgaatat catcaatcaa ctggaaaaag acggaaaagt gacacgtcca gctctaggta
     2161 tcacaatggt cgacttatct gccgtttcta ctcagcaaca agaacaaatc ttgaagattc
     2221 cttcatctgt aactaacggt gtcatcgtga caaatgtcca aacagctaca ccggctgaaa
     2281 aagctggatt gaaacaatac gacgtcatta ctaagatcga tgacaaagaa gtttcttctg
     2341 gtgtagaact tcaatctgct ttgtataaga aaaaagtggg agattctgtc aaaatcactt
     2401 actatgaagg ctcgaaagaa aaaacaacta ctgttgaatt gacgatcgat caatcggcat
     2461 taaaacaaaa tcaaaatagt gaaaattaat aaaaaagagg ttgtgacaaa gacttgtcac
     2521 aacctcttta ttttggaaat gcttctccga ctacggcatc ctcaggcaat actaagattc
     2581 ctttttttgc tggagcattt ggcaaatgca attcacgagc ggaacagatc attccatagc
     2641 tatctacccc tcgcaaagtt cctggccaga tcatcattcc atctggcatc atagctcctg
     2701 gttttgcaac gacaactttt tgtccagctt cgatattggg tgcaccgcaa acgatttgca
     2761 atactgtacc attgtctact tctgtctcag taatcgataa atggtcagaa tcaggatgtg
     2821 ggacgcatgt ttttacgtac ccgacaacga tcttcggttc atgatcagct tcaagctgtt
     2881 cttcgaatcc tgcttctttg atcaatcgat tcaatttcgc taattcctca tctgttaagc
     2941 ttacttggcc gttcccttcg attgttagat gatcggagat atggaaaaag ttccaagcaa
     3001 caacttgacc ttgttcatct ttgatccgag caacattcca tttgcgttca acagtgtttt
     3061 ctgttccttt atcgtcggct ataataacca tcaaggtgtc tccgacgtgt gctttgttat
     3121 aagcaaaaat catgttcttt cctccattat cctttgctga gatttatctt aacaaaaaat
     3181 tgcattcact gcatgaaatt tttttcatgc tttatttccg aagaagcctc ttctggaaca
     3241 ccatttattc gtaacaaggg actgtgacaa gacttttgtc acagtccctt tactgtttta
     3301 gttaattgat taaaaaggtc gtacaagttc cacttgctta attacttggc tgttcttctt
     3361 cgtcagaaac gatcaagaca tcacaaggtg aatgacgtat cacgtagctg gctacacttc
     3421 ctgtcataaa gcgttcgact gcatttagac ctgactgccc aaccatgatt agatcgatct
     3481 catatttttt tggcaatttg actgccatgg attcacgaac agaaccatat gcaataattc
     3541 cttctatatt tgtgtaatca acagatttcc catattcttt acattcttcg atcatttcct
     3601 tggctgtttc agtttgctga tcgactagat tttgatcaat aggtgcatag cccataaagt
     3661 tataaaattg ctgatcgatt acgtttgcta catatacttt cccattatta cggcgagcca
     3721 cttctaccgc ttttttgaaa gcagcaagtg cttgttcact accgtcgata cctactaaaa
     3781 tattcttata ttcttgattc atcttgaccc ctcctaacag atgtcagcct tttgttacac
     3841 tttctaggaa cgattcgatt tcctgttttg ttttacgatc tttattcact agacgaccta
     3901 attcttttcc gtcttggatc acgacaaaac ttgggatacc aaaaatgttc cattcagcgg
     3961 ctacatctat gtacttgtca cggtctactt caataaattg ccaatcagaa aagtcatttt
     4021 caatttcagg catttgcggt ttgataaaac ggcagtctcc gcaccagccg gcagtaaaga
     4081 aaaatacact ctttccagtt tctacgtagc ctgctaattc ttctaaacta tttgggataa
     4141 tcattctttg tcctctactt tctgtttatg tatttttcaa ttaaattata cacctttctt
     4201 tcgaactaac aaacattgat cctcttttta taaaaaaaat ctggcaaaaa gcaatctttt
     4261 gccagatttt atagtttcac tatcgcatta ttttccgtat acgatcgtat ttactgttga
     4321 tttatccaaa cctttcagtg tttgaatcaa catttctcgt gcagcttcaa aatcgcgaat
     4381 actgaacatc gtttgatgcg tatggatata acgtcccact acgccaatca ctgtacttgg
     4441 gattccttcg ttttgtgtat gagcagcacc tgcatctgta ccgccttttg aaacaaagta
     4501 ttgataaggg atattgtttg tttcagcgat atccagcaag tactcgcgca aacgaggaag
     4561 catgatcaaa cctggatctt ggatacgcat caatgtacct tctcctaaat gtccgaatgt
     4621 gccatttttc gtcactgtat catctgctgc agagcagtct actgcaaaga acagatctgg
     4681 cttgaattta ttgacagaag ctttagaacc acgcaagcct acttcttcct ggacattcgc
     4741 gcctgcaatc aatgtatgac ctaatgtttc attttgcaac gcttctaaag cttctaatac
     4801 cattgtacag ccatagcggt tgtcccatga tttactgata atattctttc cgttggctgt
     4861 tttgatcgtt tcagcgtaag gaacgattgt gtctcctgga aggacaccgt aactcattgc
     4921 ctcttctctt gattcaaacc cagcatcgaa caaaatatcg gttacttcta ctgatttttg
     4981 tccgcttgtt cctcgcaata aatgtggggg aacggaagaa gaaatacatg gataattctt
     5041 accagtagag gttttaagtg taaaacgctg agcagaaaca acgtaaggat tccagccgcc
     5101 tagaggtacg actttaaaaa gcccgttttc ttggatttgt gtcagcatga atccgacttc
     5161 atccatatgt gctgcgacca ttacacgggg tgcatccgct tcttcatgat ggcgcagacc
     5221 aaaaatgccg cccaaaccat ctaactgtaa ctcatctacc agcggttcca tatgactttc
     5281 catataagcg cgaacatctt gttcaaaacc gcttgttcct tgtaattctg ttaactcttt
     5341 gattcgttga aatgtttttt cttccatatg ctattcgtct aaatttcaga ctaaactcct
     5401 ctcagactta tattgatctg cggataaaat atgatttttt ctgcagaccg atcgtattta
     5461 ttatagctta ttttttctgg tagcggttat ttatttcaaa gaaaatatga gagtgtggta
     5521 aaataagcac aaggagtaac aagtgagaag aaggaggtat gtccacatgt atgaagaaag
     5581 cgaactaagc tattttaaag gcgggttagc agttggaatc ggtctaggat tattgagcgg
     5641 ggtagcttca acgatttggt atcatcgcaa aaaagtattg tcagcagacc aagtacttga
     5701 aaatatcaag tcatcttttc taaaagaagg tgcaatcgaa ggttcttgga tctcttttga
     5761 aaaggaacct attaggaaat tcgccgttcg gtcgagtgct tatcggggag gtctttctcg
     5821 aatagaagat ggcgaaactg tttattatga atttttagca gatgctcata caggaaccgt
     5881 attagaaatt tcaagaaaaa aaggtgcaac agattaatac gtcaaaaaag tgagagaacg
     5941 aatacgcacc cactattcaa aagagaggta tcatgtcctt cttttgtagt gggtggtttt
     6001 ttagatagag ctttccaaac atatattcca gtattttctt tttaaataaa tgatatttag
     6061 cgggaaaaac tagcatttcc tttgattatg aggtattttt aatttagctt ttgttttcta
     6121 tagttaagga gagtgaaaaa tggaacaaca agaaaaaatt gctgttctga ttccttgtta
     6181 caatgaggaa gcaacaattt caacagtcat tgcagacttt aaacgagaat taccagaagc
     6241 agatatttat gtctatgata ataattcgac cgatcgaaca tacgaattgg ccgttcaagg
     6301 tggagcaatc gtaaaaaaag aacctcgtca aggaaaagga aatgtgatcc gccaaatgtt
     6361 ctttgatatc gatgccgact attatttaat ggtagatggc gatgatactt atcctgcaga
     6421 agctgtacat ggtttgctcg agaaattacg ttctggtgaa gcagatatgg tcattggtga
     6481 tcgcctatca aatggaacct actttgatga gaataagaga cctttccacg attttggaaa
     6541 taaccttgtt agaaatacaa ttactagaat gtataaaaca aaaattcttg atgtcatgac
     6601 cggctatcga ggattcaatc gtatctttgt caaaagtttt cctattatga gttctggttt
     6661 tcaaatcgaa acggaattga cgatccatgc tttagataaa aaattcaagt tcgttgaaat
     6721 cccaatcgat tatcgtgatc gtcctgaagg cagcgaatca aaattgaata cattctctga
     6781 cggattcaaa gtgatcatga tgattattaa aatgtttaaa gattataagc ctttgctttt
     6841 ctttgggatt tggaccattt tcttcttttt atttggattg gtcacgggga ttcctgtgat
     6901 tcgcgaatat atgctgacaa gctttattac acgcatccct tctgcgattt taagtactgg
     6961 attgatgact ttagcacttc tttcattagt aacagggttg attttggata cagtcgtcac
     7021 gaacgctaag aaagaatatg aattgaatct atatcatgtt tatcatgaat accacagtag
     7081 aaagtaaaaa aaggatcaag actgcaaaaa taatttgcaa ttcttgatcc tctttttaat
     7141 taattatttc gccaaacgat agatggcatc tgcatagatt gctgttgctc tgaacaagtc
     7201 atcaagactc ataaattcgt ttgcttgatg cattgtatca gtatatcctg ggaacatcgc
     7261 tccataagca acgccacgtt tcaacaaacg tccgtaagtt ccgccaccga tgatttgttc
     7321 gtagcctttt tctccagtgt ggtcttcata gacttgcaat aatgtagcca ccaatggatc
     7381 atccattggt acgtaatgtg gttccattac gcgagcacct cgagtaactg ttgcgccttc
     7441 ttgccccact gtttgttcga tgtcactttg caatccgtct actgttacac cttttggaaa
     7501 acggaaattc atattgatga agttcgcttc ctctgtacca ttttcttcaa aagcaaatag
     7561 tccagcattc attgtcaatt tgcccatttt ttcatcttca taagcgactc ctagtttttc
     7621 tccataaaaa tcttcatgga cataagcagc tgctacatgg ataaattgtt tagcagatcc
     7681 taggaattca tagttatcta agaacaatgc caagaaagaa cctgcattga taccagattg
     7741 tgggcttgca ccatgtgcgc ctttaccaac taattcgatt tttacatacg tattgtccgc
     7801 ttcaatcgtt ccgcttactg gatttgcttc gatgaattgg taaaatgctt cttccatcgc
     7861 gatagctgca tctgcacttg gtacttgaag tgctgcagtt gctgttcctg gcaccatatt
     7921 ttcgcgaagt ccggaaacaa aagatttcaa gacataatct cctgcattgt cgcctttgaa
     7981 gcgtaaagct aaagaaacat ttcctttttc accattgatg attgggaatt cagcatctgg
     8041 tgagaaacca aaatctggtt cttcttcatg ttcgaaataa taagccatat cgccccagcc
     8101 gctttcttca tcgctgccga ctacgaagcg aacttttttt gatagtttca agtcaagttc
     8161 tttaatgatc ttcatcgcat agtaagctgc catacttgga cctttgtcat cgcttgatcc
     8221 gcgtgcaaag atctttccat ccttgattac tggttcataa ggatctgtgt cccagccatc
     8281 tcctgcagga acgacatcca tgtgtccaaa tatccctagc gtttcgtctc cttcgccaag
     8341 atcaatatgc cctgcatagt tatcgacatt ttttacttta aatccatcac gttcgccata
     8401 agctaacata tgtttcaatg cgtcgcgagg tccaggtcca aatggtgcat ctggtgttac
     8461 ttttgagtca tcgcgctcgc tgttgacacg taaaagattt ttcaagtctt caagcaagtc
     8521 ttctttacgt gcttctactt ctttttgcca atcaattgtc ataaagttaa tcctccactt
     8581 atttaagtct tcgtgttcat gataccacaa attacagaaa aaaatagttc aaaaaatctg
     8641 acaattcctt ttcttatctg tttttctcta aaaaaaatat acgctataat gagtaaaagg
     8701 aggtttttag atgctgataa acgaacaact agtgaaaaga gtcatcacac ctcgtccaaa
     8761 aatttcacat aaaggaacat ttggacgtat cgttttgatt ggcggcaatg aacaatatgg
     8821 cgatcattat gagtgcttta gctgccgtac acagcggagc gggtctcacg actgtagtaa
     8881 ctgatgaaaa aaatcggagt gctcttcaca gtcatctgcc agaagcaatg attgtagttt
     8941 ggacggatac tgaccagttt ctttccgtaa taaagcaagc agatgtactc ttgattggtc
     9001 ccggattggg tacttcttct aagagtttgg aacgtttcaa acaagtacta gaaaaccaat
     9061 caaaagaaca atggctggtg attgatggct ctgctttaag tttacttgct gctaatcatc
     9121 ttttgctacc ctatcccgaa caaactgtac ttacacctca tcaaatggag tggcaacggt
     9181 tgagttctct accgatacaa gaacaacggg ataaagaaaa caaaaagaag caacaggaaa
     9241 tcggtgctgt gctcgttttg aaaagtcatg agacaaaaat ttatggtcac cagcacttct
     9301 acaccaatcc tttaggttct gctgctatgg caacgggcgg catgggtgac actttagctg
     9361 ggatgatcac aggattttta gcccagtttc ctcaaaatga ccaaacgatt gcagcagcta
     9421 tttatcttca tagtttcatt ggagaccagc tggcaaaaga acaatatatt gttttgccga
     9481 cacaaatcag cgaagcactt ccgaaatgga tgaattattt ttgtaaaaaa tcaatggaaa
     9541 acgaggcatg agtcatggag aaatttacag gttatgtgcg agaacctttt tattatgaaa
     9601 cagatcaaat ggggattatt catcacagca attatatccg ctggtttgaa gaggctcggg
     9661 tagccttatt ggtacatcta gggtttccct ataaaaaaat cgaagaatct gggatcatca
     9721 tccccgttct ggaagtaaat tgcagctata aagaaatgat ccattacgga gaaaaagtga
     9781 gcatccatcc aactatccaa aagtatacgg ggacacggtt ggattttagc tacgaaattg
     9841 tgggaactga agacaaaaaa ttaaaaacta ctggcagtag taaacactgt tttttacttt
     9901 ctgattcgca acgattagtg aagctgagca aagtcaatcc ggagttagat cagttattcc
     9961 aagcgtacgc ttctttggat gatgtaaatt cttaataaag accgtctaag catttttctt
    10021 gtattgtgtc gagtagttcg tataatgaaa agaaaaaagc aggtgatccc catgtatgtc
    10081 gttaaagtaa tgcacggtta catcgataaa actggctgtc gcactcggga aaaaaactta
    10141 gacaatctgc tcattttcaa agacaaaaaa gaatccgaag cttttgctaa aagaatcggc
    10201 ggtcgtgtca aaccgatcca agaggtacga ccagattgat tcaatatttt tcttttcgag
    10261 gctctaatag gcatgcctat cagggctttt tttgcagact gtgtttttat gcccacacga
    10321 tttgtaagcg atttatgtta taatagatta tatctgcata gaaaggaagg ttaaatggtt
    10381 atcaataatt tgcaacagca agcttatgaa tctatacgta aacaaatcat ttattccgag
    10441 ttagcacctg gaagtaaaat ctccgataaa gtgttagaag aacaactagc tatcgggcgt
    10501 acaccgattc gagaagcact gatccaactg cgcaatcaag agcttgtaaa aaccattcca
    10561 cagtccggca cttatgtttc aaaaatcgat atccgatctg cttcattagc ccgttatacg
    10621 cgggaaaagt tggaaaaacc gatcctgcaa gaatgcagtg cgaaaatgac caagcaagat
    10681 caagctaaac tagaaaaaat actagaacaa accgatcctg caagaatgca gtgcgaaaat
    10741 gaccaagcaa gatcaagcta aactagaaaa aatactagaa caaaccgatc aggcagtaat
    10801 cgctcaaaat aaaaaagctt tttttgattt ggatactgat tttcaccgaa cttgttatga
    10861 aattgctgga aaacgagaaa tttgggattg gctagaaagt tatagtacgc acttgaaccg
    10921 tttcagatgg ttacgtttga cgatttcaga attagactgt ggtcgagtat tagatgaaca
    10981 tcagacaatg cttcaatcaa tgattgatca taactttgat gaagtcggat ttctttgcac
    11041 gatgcattta catatgatta tcgaggagca agaatacgtg attcataatt accccgatta
    11101 ttttgaagat attcaggaca gacccatcaa ataagaggtt ttggaatcag cgttttgacc
    11161 tgagtattag agaaagaaac ggcaaaatag ttcctcatat tttgccgttt tttttgagct
    11221 aataccgagg gtcagctgat tgcacaccgt ttattgagag gttgtgaaaa agctgccctg
    11281 catcaagtaa taagaacagc caagtaaaaa tagcttctta tgtttttaac ttggctggac
    11341 ttattaccgc agatgcaagc ttttgaacac cgttttccaa gaggtcgtga gcctgccgtt
    11401 catctccgag tcacaaggaa caatttcaaa aaacagcttc tcatgtttgt tgaatatttt
    11461 gacttgtgac cgaggagttg acaggagaac atcgtttatt cggaaaaaaa ggactgtgac
    11521 acgacttttg tcacagtctc tttatttgta atctaaatca tactccgtac atcttttttc
    11581 tatttttttt atttcttcta agatcccttg gctacgaatt tcattatatg gccaaagtaa
    11641 gactgcctct aatacataag gcgaaaaatc gctttgattc atctcttttg aaaaactcat
    11701 cgctgctatc tcgctcaatg cacgaatgtt tgattgatgt gtataaaaag gcaaccgcca
    11761 cagcacaatt ttttcgcttt gcgtataaat tcccttaaca gaagcttcga tgtggtgaaa
    11821 tagttcatgt ccaataatca aagcttgcgc tgtttcttta gagatcgaag ctagtcctga
    11881 acattttaat ttttctaacg gctccgtcat caatcgaatc ccttcatctt ctgcataagt
    11941 tgcaaaagtg attcgttcgt ttatttgctc tgattcacag gaaacgattg gaacagccat
    12001 ttgttcacaa atttctccta catcggcaaa agggtacttt tttttgatgc tacatgccgt
    12061 ttcgatacca caatcgattg attgttgtat aagaattttc tgcttggaca aaggaatctt
    12121 tttccaaaat aaatctcgcc gaaactggta gaatccccaa atttcttcgg ggatctgttg
    12181 taattcgttt agtaactctt ctaacttatg tttgtttgat atttccggca acgacgatca
    12241 cttcttcctc cggttccata gtagatactt ccagatccat caaaagctgt agttgttcca
    12301 gatccgtcgc tgtaaaatca gagactcttg ctcctaagca gagataaaat tctggtcggg
    12361 caatcaattc agtttgataa actgccatat cttcccaaag ttgttcgaca gctgtcatat
    12421 aattggctgc agttgttttc atacatgaag catgtccacg ttggaccttg ataaatcctt
    12481 tttgatcgat gatccgaaga cttccagcat tttttgaacg attctgttcg ccgcatacat
    12541 aaaagaaagg tgtattttcc aataagcaaa cttcattttt atttaatcgc atatcctctg
    12601 cagcaagctc tagtgcttct tctgttgtga tctccttggt tagatcggtt gctttcactt
    12661 ccgtagaacc agtagcgata gccgttactt ttgaagtctg tggatcgatt tctacgtgga
    12721 cttcgatcga ttctggtgta gcgcctgatt caatcgcttt attcatcgct tcatttttca
    12781 acgatcgtat atcttcttta cttggcgaag ggatgatacg ttctacaaca tctcggacca
    12841 ttgcaagtgc tacaccaata gaagaaatca cttcggcgtt ttcaggaatc gaatatttca
    12901 cacccatttt attactaaag taagtaatca atgaggcagc gccgccccct acacctacaa
    12961 gagaaatctg atctttctcc aagtgatact tgtctgccaa ttctaaaatc accggttcaa
    13021 tcttagcata ggatttttcc atgatctgtt ctgcgatgtc ttcaaccgtc gtatgacaat
    13081 aatcagctaa tgcttggatg gcttttcgtg cagatgggac atttccataa gaaaaatgtt
    13141 cttcttgcac taatccaagg acattggctg cacatgtatt tgtaatagtc acttcttctc
    13201 cgtcttccat cactaccttg acgtaatcag cagggtctcc ttctttagga gagaaaaatt
    13261 caaccttagg acctttgatt ttttctgtct ccgtaaatac tgcataatct agaccggcaa
    13321 tatgtgcaga acgtggaccg acatcgatga ttcctgactg attagcccgt accatcgaac
    13381 cgcctgccac acctagaacc cgaacatcta atgaagagat atatgttggg tggccaccta
    13441 caattgaata atcgattgct ggacgtccat ttttaatgac cccgatattg gttgttgttc
    13501 cgcctacttc aaaataaaca ccattagacg ctctcaagta catcaaagag cccatgactg
    13561 atgctgctgg accggaaagc atggttaaaa caggtcgttt tttcatttca ttgatttcca
    13621 ttactccgcc gtctccgcgc ataatcatca aagaaacatt gacccctgct tctcgtacgg
    13681 aatcttctgt agaggttgcc gtatctagca tttttggtaa aatagatgca ttgattgctg
    13741 ctgtccgagt tcttcttgtc aatccatata attttgtgat gtcagaagcc atcgttgtag
    13801 ggatcgattt taccgatgct gcttcataaa caactcgttc tggttcccca ttatctactc
    13861 caaatgccat agaagaaacc aagacctccg ctctttctct ttctaaagaa gaaatcgttt
    13921 ctgaaacacg gtctacattt aagtgtttga caggtaagaa tgcattgaca atctctattt
    13981 ttttcttatt ccctaaatcg atatcgttca atctagtttg tcgttttgct aaaaaacctt
    14041 ctaatccacc ttttgccatc ccgattacgc cgacttttgc tacgtctcct tcaatcagtg
    14101 cattagtcgc ttgtgtggta gaatgtgcaa caaacacaac atcttctgga ctgatattat
    14161 tttccagcaa acaattttga aaagactgaa cgacacctgc ggctactcca cggacatcat
    14221 cgtgggttgt tttcactgaa gattttccga tgatttcgtg cgtcgcatta tcgattgcga
    14281 ctgctttcgt atgtgtaccg ccaacatcaa ttcccattcg cacaaaacgt ttcattttct
    14341 tttcctccat tcatctaatc gtttttatcg acgttttgtt tactaggcgt tcccgtacaa
    14401 taagaacgtg atcaggcata agatcgctcc gatgatccaa ccagttggga tggatttctt
    14461 catatattct cgagaagtta cttttgtata acctaatccc caagcaaccc aagattgtgt
    14521 aatatctaaa tgctgaggag caatcgttgt gattgcaaat aacgggaaca aaaaggctgg
    14581 cgacatcgtt ggattaacag ctaatacgac agctaaaata gctgagcctg aaccgactag
    14641 attcattgga ccgcggaaaa atcctagtgg tgtcaatata gcaaatacga ttgccaagac
    14701 taatggtgat tgcggtatcc aatctccaaa aatagctgaa aaataaggag aagcatacgc
    14761 agctgaatta ttgaacatcg ctaaggtcat caagaaacca accattggcg caacatctac
    14821 tgcgccatct gtaaataatt tcgcaaactg acgacagatt tctttataac ctccgcgcaa
    14881 ttttccagta aacagcaatg cccagatacc tgctagaata aaacctaaga tgattggaag
    14941 atctaaaaga acgaccccta aaactggtaa taatacggct aaccatgaga tcattggtgc
    15001 gtcgccacct tcagctggta cgtttgctgc cattgcatag cgtttctttt tgttcattga
    15061 tatattagcg accgttagta ccacaactaa gcttacaatc atcccaatca taccaatttg
    15121 gaaatagtca ttataagtat agctttcagc cgctggattg aaaccagcat aaatcgtttg
    15181 atattgtttg aaattgacga tattagcaaa gattccagcc ataatcgagc ccataaatga
    15241 gaacagtgca accggtactg aaatacctaa tgacatcaag attggcaaca caataactgc
    15301 aatagaaatc actggtccga ttcctgtcat cgacatgaat ataacagcgg ttacaataca
    15361 tagtaagctc atcgtgattc ttggcttgtc ccctccaagt tcaactacct ttcgaatcag
    15421 ggtagctgcg attccgctat ctactagaac acgcccgaaa aaggcaccaa agaagacatt
    15481 taccaagatc gatttggcgt actcagctgg ccccgtcgta tagatatgcg tcaacacatc
    15541 aattactcct tgcccttcca tcgccgggtt tggtgaaaac gtgtttccga ctagcgctaa
    15601 tcccatccat acagtagcca tgatggcaaa gccgaccatc aagttgtagc cttttacaca
    15661 gtaccagacc atgaaaaaga atgagataac taacaaaata ccaataataa tatcactcat
    15721 gttattcctc cgtccatttt gtttttctga taagcaaact aatatatcag tcaaactcat
    15781 tctaacttat gtagttagcg ctttcattaa agggtgacca taaatgttca gttttcaatt
    15841 aaaaaacctt aattatgcta ttttagactt atacgttatt gtcctgtttc atttgatgga
    15901 ggattgtaaa taaaaaaaga ggaaagaaca taacttttcg ttatcttctt tcctctttat
    15961 aattgtttag cttatttagc caaagcttct tttgcttggt cagccaatgc agtaaatgct
    16021 gctgcatcgt taacagccaa gtcagctaac attttacggt tgatgtcgat ttcagcaact
    16081 ttcaaaccgt gcattaattt tgagtagctc aagccattca tacgagctgc tgcgttgata
    16141 cgagcaatcc acaatttacg gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag
    16201 ttgtatgaat tcattacttg ttcttttgct gttttaaata aagtgtgttt tgaaccgtag
    16261 taaccttttg ctaattttag cactctttta cgacgtttac gagttactgt tccaccttta
    16321 acacgtgcca tgtataattc ctcctaataa aaataaatct tcagattctg ttatttgtac
    16381 ttcttatttc atttttgcta gttgttggcg aatacgtttg aagtcgccac tagaaaccat
    16441 gcttgcttta cgcaattgac ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt
    16501 aaacgcgcgg aagcgtttca atccgccttt accagtacgt ttgaaacgtt ttgctgatcc
    16561 gcggtgtgtt ttttgttttg gcatgactat atttcctcct caaaatcttt cgttatccga
    16621 ctatcggctg tccaacagcc agttagtcat tcaaattact tactgtcgtt tttcggtgcc
    16681 agcgtcaaga acatgctgcg tccgtccatt ttcgcttttt gttccactgt tgcaatatcc
    16741 gcagtttctt cagctaagcg attcaagact ttttgaccaa tttctttatg ggtaatggca
    16801 cggcctttga aacggataga agctttcact ttatcgccct tttccaagaa cttacgtgca
    16861 ttacgaagtt ttgtattgaa gtcattcaag tcgatcgtag gacttaagcg aacttctttt
    16921 acattgatca ctttttgttt tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg
    16981 tatttaccgt agtccatgat tcgcgcaact ggtggtttcg caccaggggc aactaacaca
    17041 agatccaagt ttgcttgttc tgcaatattc aatgcttctg cttttgtctt aactcctaat
    17101 tgctcaccat ctgaaccgat caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc
    17161 atatcctttg ctatggtcat tcacctccaa atattatgag agaaagaaaa gcttaccgaa
    17221 taaacggcaa taaaaaaacg agttctctta cagaacccgc gcgtacttca cctaatagaa
    17281 ccagtgaaaa ctatctagcc cagtgacatt atcaactaag gcgagaagcg ggtgcttctg
    17341 cttttatttc tcaaccatca tagtatagca aaaactaatc tttttgtcaa tgtttttcat
    17401 acagtttgtc ttccttttct gaaaatgatt agattagcag actttctagc agtgattccc
    17461 gcttctttgc tatactaaag aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt
    17521 ccaccttctc ttcctcttct tacaaaggga gagttttatt tagaagatga ggagatcttc
    17581 tctggtcttc tgatcaacgg gggtgaccaa agctatctcg atgccaaaaa tatggtatta
    17641 agagagagtc atatccaaaa acttacgatg caaaaaacag ttttacgtca ttttgaatgc
    17701 agtaatgtca tttttgaaaa atgtgatttt tccaatctgg agtggattgg tggcagtttc
    17761 caccaagtcg tgtttcatca atgtaaatta acaggtacga attttgccga aagttatctt
    17821 cgagactgta ctttcactga ttgtattgct tcgatggctt cctttagtag tacaaacttg
    17881 aaagctgtct ccttcgatca ttgtcaatta gaagacagcg aattttatga ggtcacttgg
    17941 aaaaaccttt ttttgtctga caatcagttg accggttcga actggtttcg aacttctctg
    18001 aagggattag actttacgac taatttcttc acagcaatcg ctttatctca agattctttg
    18061 gcgggactaa tcgtcaacca agagcaagca cttgtgatcg cccaagccct tggtttgcag
    18121 atcaaagact agatcaacga atccgccgcc acagtgcaat aagcatccaa atagataaga
    18181 ccaaactgat gatcaaggcg attccccatc cgaaaacgga atgggtaaag ggtaactgga
    18241 gattcattcc gaagaatcct gtcacaatag ttggaacggt caagatcagc gaccagaccg
    18301 tcaaaaattt catcgtgtca ttcaagttgt tattcagcaa attattataa gtgccagaga
    18361 gctggtctaa aatctgcgaa gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca
    18421 gcgcatcgtc caactcttct ttttcctttt ctgacaactt gcgataaata gccaatgcct
    18481 tgatttgttc taaaagtact acattttgtt ttgttcctgt aactaagtaa accaaaccga
    18541 tctctaaatc tgaaagttgc agcagattct tgttcgttgt tttttctctt aatttcagat
    18601 taagccgctg acgttcacta ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca
    18661 aaaacaatgt acgaaacaat aaactgtata cactcatgtc tggcattcgt tccaaaagcg
    18721 atcgaattag tcgaatcacg tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt
    18781 cttttaaaat aaaagcgata ggactagttt cataatgatt atcgactttt tgctggtgcg
    18841 gaacatttga tacaagcagt aacgcttcat caaaagaatc gtactctacg cgcgcccgtt
    18901 catttttatc tagagaataa gtcagcatct cgtcactaat gccatattct tcttttaagt
    18961 ttttaacagc tagcacatcg ttttgctgta cattgatcca aaaattacgg tcttgattaa
    19021 aatagtgttt ttcccgcata ctctatcctc ctgacatttg tttccagtat aaagaaaaaa
    19081 gtcgctcaaa taaaggttta ccctttattt gagcaactgt aaatcaaatg gctactttgg
    19141 tttggcgaat ctctgctaat ttttcttcga tcattttcaa gacttcttct tttgcttgag
    19201 gatcttccac aaaattatag cggtctccat cgatttggat ttttggactt tctttgtatt
    19261 cttcatacca ttgatcgtaa cgtctgttca attctttgta ataatcgtat agacttggat
    19321 caaaggacaa ttgttcatac gaacgaccac gtttttcgat acgctccaac atcgtatcaa
    19381 aagatacacg aatatgaact aataaatccg gatgtttttt atgggcagca tagggaagtt
    19441 cttccatcat gttttccagc agttcatcat acacacgaac ttctgtttct gttgctcttc
    19501 ctagatctgc atttagatgg aaaagcagtg aatcttcata aatcgaacgg tccaatacat
    19561 tattgtcatc tttcattgct tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt
    19621 gcagcaaaaa agcatactgt tcagggtttg cataaaataa gggcaagact tcattatcat
    19681 caatcgattc ataaaatgcc tgactatcaa agtgttcagc catcatttct gttaaacttg
    19741 attttccggc accaattgta cctgctaaaa cgatcacgct catttacctc cagctcactt
    19801 catcactaaa tattgtgttt aactccaacc tttttaaata tacccccaat atctagtagt
    19861 gtcaattgta tttttttcta gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa
    19921 gtgttttttc tttagaaaac gtattcttag ttttacttct gagatggttt tagtaaaata
    19981 aaggaggaat gttttttgag acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc
    20041 gttttgggcg ctttattttt tgcttaaaaa tcacttttac gtgagaagga gttagattat
    20101 ggcaaactat ggccaaggag aatcaagcgg aaagatcata ttgatgggcg agcacgcggt
    20161 tgtttatgga gaaccagcga ttgcctttcc tttctatgca acaaaagtca ccgcattcct
    20221 tgaagagctg gatgcaatgg acgatcaact ggtttcttcc tactattcag gaaatttagc
    20281 cgaagctcct catgcattaa aaaatatcaa aaaattattc attcacttaa aaaaacagca
    20341 tgacatccaa aaaaacttgc aactgaccat tgaaagcacg attcctgctg aacgtggaat
    20401 gggatcaagc gctgcagtcg ccacagcagt cactcgtgct ttttatgatt acttagcatt
    20461 tcctttgtct cgtgaaatac tattagaaaa tgtccagctt tcggaaaaaa tcgcccatgg
    20521 taatcctagt ggaatcgatg cagccgctac tagcagcttg cagccgattt attttacaaa
    20581 agggcatcct ttcgactact tttctttgaa catcgatgct tttttgattg tcgctgatac
    20641 aggaatcaaa ggacaaacaa gagaagccgt caaagatgtc gctcacctct ttgaaaccca
    20701 gcctcatgaa actggacaaa tgatccaaaa attaggatac ttgacgaagc aagcaaaaca
    20761 agcaatcatt gaaaattcac cagaaacgtt ggcacagaca atggatgaat cacaatcact
    20821 tctggaaaag ctgacaatca gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga
    20881 taccggggca ttgggtgcca aattaactgg cggtggacgc ggtggctgta tgattgcatt
    20941 agcacaaaca aaaacaaaag cccaagaaat cagtcaagca cttgaagatg cgggtgctgc
    21001 tgaaacttgg atacaaggat taggagtaca tacctatgtt taaaggcaaa gcacgcgcat
    21061 ataccaacat tgctctaatc aaatattggg gtaagaaaaa tgaagaactc atccttccaa
    21121 tgaacaacag cctttcgtta acattggatg cattttatac agagacagaa gtcatctttt
    21181 ctgatagcta tatggtagat gaattttatc tagatggcac cttgcaagac gaaaaagcaa
    21241 caaaaaaagt cagtcagttt cttgaccttt ttcgtaaaga agccggcctc tcactaaaag
    21301 cttcagtaat cagtcagaat ttcgttccta ccgcagctgg attagcctcc tctgccagcg
    21361 ggctagctgc tttagcaggc gcttgcaata ctgctcttaa gcttggatta gatgatctct
    21421 ctctttcaag atttgctcga cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt
    21481 tcgtcgaatg ggaaaaaggc catgacgact taagttctta cgctaagcca gtcccttccg
    21541 attctttcga agacgattta gcaatggttt tcgttttgat caacgaccag aaaaaagaag
    21601 tgtccagcag aaatgggatg cgtcggacag tcgaaacatc cagtttttat caaggctggt
    21661 tagattccgt tgaaggggat ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc
    21721 aacttctcgg agaaacgatg gaaagaaacg gactaaaaat gcatggaacg actttagctg
    21781 cccagcctcc attcacttac tggtctcctg acagcttaaa agcgatggat gcggtgcgac
    21841 aattgagaaa gcaaggcatt ccatgctact ttacgatgga tgctggccct aatttcaaag
    21901 ttttagtgga aaacagccat ttatctgagg tacaagaaac atttaccaaa ttatttagca
    21961 aagaacaagt aattactgca catgctggtc caggaattgc gattattgaa taaagggaga
    22021 tttttatgat tgaagtatct gcaccaggca aactctatat tgccggagaa tatgcagttg
    22081 ttgaaacagg ccatccagca gttatcgctg cagtcgatca attcgtgaca gtcactgtag
    22141 aatctgcacg aaaagtcgga agtatccaat ctgcacaata tagtgggatg cctgtacgtt
    22201 ggacaagacg caacggagaa ttagttttag atattcggga aaatcctttt cattatatcc
    22261 ttgctgctat tcgcttgact gaaaagtatg cacaagaaaa aaacattctt ttatcctttt
    22321 atgatctgaa agtaacaagt gagttagaca gttcaaatgg ccggaaatat ggattgggtt
    22381 ctagcggtgc cgtgaccgtt gcaacggtca aagccttgaa tgttttttac gcgttgaatt
    22441 tatctcagtt ggagattttc aagattgcag cactagccaa tttagcagtt caagataatg
    22501 gttcctgcgg cgacatcgct gctagctgtt atggtggctg gatcgctttc tcaaccttcg
    22561 accatccttg gctccaagaa caagaaactc agcattctat cagtgagtta cttgccctgg
    22621 attggccagg tctatccatt gagccattga ttgctcctga agatttacgt ttattgattg
    22681 gttggacggg tagccctgcc tctacttctg atttggtcga tcaagttcac cgttcgagag
    22741 aagataaaat ggtggcttat cagcttttct taaaaaatag tacagaatgt gtcaacgaaa
    22801 tgatcaaagg gtttaaagaa aataatgtaa cgttgatcca acagatgatt cgaaaaaacc
    22861 gacaattact gcatgattta tctgcaatca ctggggtcgt catcgaaacg cctgctttga
    22921 acaaattgtg taatttagct gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg
    22981 ggggcgattg cggaatcgta attgttgacc agaaatctgg cattcttcct ttaatgagtg
    23041 catgggaaaa agcagaaatc actccactgc cgttacatgt ctatagcgat caaagaaagg
    23101 aaaaccgatg aatcgaaaag acgaacatgt ttcactagct aaagcctttc atgataaaca
    23161 aaaaaacgaa tttgacttcg tacggatcat ccataaccct ttgccgcaaa tagcagtttc
    23221 tgatgtcgat ctaagtacac aggcagttgg gtttacacta agcagcccat tttatatcaa
    23281 tgcgatgact ggcggaagcg aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc
    23341 aagagaagca gatttgatga ttgccactgg ttcagtcagc gctgccttga aagtcccttc
    23401 tttagccgat acttatacga ttatgcgaca agaatatcct cacgggaaaa tcattgccaa
    23461 catcggtgca gggacttctg tcgaaagagc caaagaagct atccgattgt ttcacgcaga
    23521 tgccttacag attcatttga atgcacctca agagttggtt atgcctgagg gagaccgtga
    23581 ttttaccaat tggaaagtac ttattcaaga aactcaaaca gccattgacg tccccctcat
    23641 cgtaaaagaa gtcggttttg gcatgacaag agaaacgctc aatgatctag ctgctttagg
    23701 agttcataca gttgatatca gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc
    23761 ccgtcgctcc aagcgggaac tgagttattt agctgactgg gggcaatcga cagtctcttc
    23821 attgcttgaa gcaaatgaag cagacacttc tatggaaatc ctagcctccg gaggtatccg
    23881 caatgcctat gacatattta aagccctttg tctcggcgca aatgccgtgg gtacttcagg
    23941 aaccgtactg acgcatctga tgaatcacgg cgtagaagaa actattatac tgatgaagca
    24001 atggcaagaa gagcttcgtc tgctttatac tatggtcggc gcaacgaata ccgcggccct
    24061 acaccaacaa tctctgatct tttccggacc agtgaaggat tggtgcgaag caagagggat
    24121 cgacttagtg aagtatggaa gacgcacaga aaagagcgtg ggacaaaagt aaagaacact
    24181 tttgtctcac gctctaaaca cgtacaaacg gcggaagtaa aagcaactcc ttcggaaata
    24241 agctgaaatt cacaaaaatt tgaaaagcaa ttttcgtgaa ttccttctta tttcttggag
    24301 ttaaatgctt ctgtcccagc ctcttttttc gaataaatga tgtttaatca gctgtccttc
    24361 ggtattcgtt caatccgctg atttcacaac ctctccacta aacagtgtac taaagctctt
    24421 ttaaggttgt tttccgatat aggcaagtat gcctccgtcc acatacaaga catggccgtt
    24481 aacgaaatca gatgcgctag aagccaagaa gacagatggt ccagccaaat cgattggctc
    24541 gccccagcgt gctgctggcg tacgtccaac gataaattga tcgaatggat gacgttcacc
    24601 gttttcttgt gtttcgcgta atggagcagt ttgcggtgta gcaatataac ctggtccaat
    24661 tccattacat tggatattgt attgaccgta ttcagaagcg atgtttttcg tcagcatttt
    24721 taatccgcct tttgccgcag cataagcact gaccgtttcg cgtcctaatt cactcatcat
    24781 ggagcagata ttgatgattt taccgccgcc tttttcgatc atatccggaa taactgcttt
    24841 tgaaacgatg aatggggcat ttaaatccac gtcgattact tggcggaagt ctgccgcatc
    24901 catttcgatc atcggtgtcc gtttgatgat ccctgcattg ttgactagaa tatcaactga
    24961 acctacatct tctttgatct gttgaaccat cgcttgaacc gcttcttcat cggtcacatc
    25021 gcagacataa ccttttgcat cgatgcctgc ttctttataa tgcgctatac cttcatcaac
    25081 tgattcttga ttcaagttat taaatacgat ttttgctcct gctgtagcta aagagcgtgc
    25141 aatctcaaat ccaataccat agacagcacc ggtgactaat gctacttttc catctaaacg
    25201 aaacatatcc atactaaatt ccatcatttt ctctccctta tgcttatttt aattgatcca
    25261 tttgcaccat atccatgtct gtataggtga tattttctcc gcacattgcc cagatgaatg
    25321 agtaattgct tgtacctaca cctgaatgga tcgaccagct tggcgaaata atggcttgtt
    25381 cattgtctac aactaaatgt tttgtttcat ctggtttgcc catcatgtgg aacacacgtg
    25441 tatcttcatt ggcataatca aagtagacat aagcttccat acgacgttca tgtgtatgac
    25501 aaggcatcgt attccaagcg cttcctggtt ctaaaatcgt ataacccatt tgtaattgac
    25561 agctttcgca tacatttgga tggatgtatt ggtagatttt acgttcattc agtgtcaggc
    25621 cttcaccagt ctccattggt tttacttggt cgatactgat ttttacattt ggatatttat
    25681 gatgagctgg aacagaacta atatagaatt tagctgggtt ttcagcatct tgagaagaaa
    25741 atacaacatg tcgtgtttct tttccgatat agtatccgtc ttgttttttc atttcttctt
    25801 tttgcccatc gatctcgatg aatcctggtc caccgatatt gatcacacct agttcacgac
    25861 gctctaagaa gtaatcaaca cctagttctt tgtctaatgt gatttctaat tctttttcag
    25921 taggtgtcac tccaccaaag atcattcgat cgttgtgcgt ataagtcaaa ctgattttcc
    25981 ctggttcaaa taccttttct actaaaaatt cttttctcaa ttgttccgtt gaataatgac
    26041 ggatatcttc tggactgtgt gtataacgtg tttccatttc ttgcattttc ttctactcct
    26101 tctatcgaat gatttttcca gagccgcatg ctaaaaattg tgcgacttct tcttgactaa
    26161 attggttgca gtccccatgg atcgtatgct tcaaggcaga agctgccgta gcaaaatcaa
    26221 tgatttcttg cggagacata ttggataaaa tcccatggag cacgccacct gcaaaagcat
    26281 ctccgccacc tactcgatcc acgattgggg tgattctatg gacttgggat tccacatatt
    26341 catggttcat ccacaatgtc cccttcaatt cattttcact tgctgaatga acggtacgct
    26401 tggtcgaata aaacacctga atattcggga acttttcctg cattttttga taataataaa
    26461 ttagaggttg ttcttctgct tctggcgctt tctcgatacc taacaaataa agagcatcca
    26521 tttttccagc tgagcagata tctactaaag gtaaaagctg atggattact tcgctggctt
    26581 cttgctgtgt ccataatttt ccacgatagt tgatatcgaa gctgatcagg cagccagctt
    26641 tttttgcagc ttctatcacc tgtttcgtca ttttttgcca ttttttagac aaagctgggg
    26701 tgattcctga aacatgaaat atatctattc cttggaataa ctcctctagc gaccattcca
    26761 aatgatccat ttcagcaaaa cttgatccgg cacgatcata gatcactgag gcggcgcgtt
    26821 cgccactacc agtttccata taataagtac ctagtcttga tccaccagat aatacctgtt
    26881 tcgtatctac tccataacat tgcaagtgct ttttgactgc ttttcccaat gcgttatcag
    26941 gtacttttgt agcaaaaatc gcttcatgtc cgtaattagc taaagaaatt gctacgtttg
    27001 cttcccctcc gccataatgg gcagcgaatc gttcacttga atcgatacgg gttccctgct
    27061 ctgtggacaa tcgcaacatg atttctccga gtgtcactac ttttcccata agcagctacc
    27121 tcttgatttc tttcattttt gctgcatact gttgcgcgac ttccgttact ttgtcaaaat
    27181 ctcctgtagc tgcgggtgct aaaaggttcc cacctactcc gactgttacg acaccagcat
    27241 cgaaccactc tttcatattg tccagactca caccgccagt tggcatgata ttgacttgag
    27301 gcatcggcgc cttgaaggca gaaatgatac ttggtccata agcgcttcca ggaaatagtt
    27361 tgacgatatc taccccgctt ttcagcgctg ttttgatctc ggtaatagtc atacatcctg
    27421 gtaagtaagg aatttgatac agattgcaca tctctgccgt ttcttgatca aaggaagggc
    27481 taacaatata ctctgcgcca gctaagatag ccaagcgagc tgtgacagcg tctaatactg
    27541 tgcctgcgcc aatcactatt tctggttggt ctttgtagaa tgacaaaagt tctttgatta
    27601 cttgatctgc ttgagggact gtaaaagtca actcaatacc agttagacca cctttgatga
    27661 ttgcgtggca agcattcaaa gcttcttctt tgctttttcc gcgtaccacc gcaatcactc
    27721 cggcgttttt caatcgagat aaaatatcaa tttttttcat cttctccacc tatttcctat
    27781 ttagaaattt tattttataa cttggaaatc tatacccata ttatacggca gaaaagaaaa
    27841 attgcaaacg cttttttcat tatttttgaa ttctttgctc gatttcgctt tttgtttgct
    27901 tgagggcact gattatttgt tcttttgtct catctgttaa acgatatttc ggcatactta
    27961 cactaaatgc accaatgatt tgttcgtttt tcacgattga ggctcctata caaaaaatat
    28021 cacgttcgat ttcttcatcg tcaaatgcga tgtgggtttc ttttaccgtt ttgatttcct
    28081 ttttcaaccg taatgcattg gtgatcgtat attctgtgta agggatcaac ggatgctttt
    28141 caagataatc agtgtactgc tcttcactga attctgctaa tacggctttg cccatcgcag
    28201 aattgtataa tggtcgggtg attccgattt ttgaagacat gcggatcgtt tgatgttttg
    28261 gatctagttt gttcacgtac aagatctcat tgttgtccaa tacccctaag tggatagtct
    28321 catcgatctt ttgctgcagc tgttctaaaa acggtaaagt caactctacc aaatcgattt
    28381 gttcaatgtt tttattcgcg tagcgaataa gctttgcacc taaacgatag tttttctctg
    28441 aatttttatt tacgtagccg atcatcaaca aagtatctag tattttcaag actgtagaag
    28501 aggtcattcc cacacccttt gcgatcgtct gcaaagagac atcttggttt tccgctaaat
    28561 agtctagtat tttagaggct ttgatcaata cagttccata aggcttttta ttttccatat
    28621 aatattctcc tatttcttta taagaaaaat tatagcggaa ataccatcaa tttaaaagag
    28681 atatccagat ataaactacc atttctcttt acaggcaatt taatgttgaa gatccgcttc
    28741 gtttttacat caaactttga tataaagcaa tgatttcttc tactgtcgtt tctcttggat
    28801 ttcctggtgt gcatacatct cgcaaagcat cttctgcaat tgctggaata tctgcttctt
    28861 ttacacctaa ttcagaaatg gtttctggga ttccaaccgc tttgctcaac cgatcgatct
    28921 caccacaagc cgcttctctt acatcttcta aagacatttc agctgctcct ttaatcccta
    28981 gaacaagagc gatctcacgg tacttttctc cagtatattc tttgttatat ttcatgacag
    29041 ttggcaaaag tgctgcacac gctacgccat gaggaatgtt gtaccatgca cttaacgggt
    29101 gagccatccc gtgtaccagt cctaaaccga catttgagaa gcccattccc gcaatatact
    29161 gtcctaatgc cattttttct cgcccatttt gatcgccatc tacagaagct tccaaagaat
    29221 ggccgatgat ttcgattgct tttaaatgca gcatatcagt catttcccaa gcgccttttg
    29281 tgataaaccc ttcaatagca tgcgtcatag catccatacc tgttgatgca gccaattttt
    29341 tcggcattcc catcatcata tcgctgtcga tgaacgcgac gattgggata tcatgaggat
    29401 caacacaaac aaattttcgg tgatttactt catctgtgat gacgtagttg atcgttactt
    29461 ctgctgctgt tccagaagtc gttggtatag ctaatatagg taaacatgga tttttcgtat
    29521 cagcaacacc ttctaaacta atgacgtctg aaaactctgg attcgtcaaa ataatcccaa
    29581 tggcttttgc cgtatcgatt ggcgaacctc caccgattgc gatcaaacag tctgcctctg
    29641 cctctttaac aaaagccact ccatctttta cattttggat cgttggatta ggtacgatcc
    29701 cgtcgtataa agcataagcg attgctgctt tatctagaag ttctgttact tttgttgcta
    29761 taccgtgctc gatcaatcct ttgtcagtaa tgacagctgc tttgccgaat cctcgctgct
    29821 gaaattcagg gacgatcgac tcaatcgctc cttttccaaa atacgatgtt tcatttaaaa
    29881 tcattcgatt actcattttc tattcctcct aatttgtatt atttttcaca ataaaaacga
    29941 aagaacccca agcaaccggt tgcccagttg cttgttgcta cagttcaaag actggcatca
    30001 attcccaact gactggagag gcatctggat ttgtctccat gatatcttcc atgtacttcc
    30061 accatttttg attgatagat gtatttgcca ttttttccca taacttttca tcttcgatca
    30121 ctaagtaacc aaataacaaa cttgtactcg gatcaagata gatcgaatag tcgatgaccc
    30181 cgtgttcacg aagtgctgta cgcatttctg gccaaagttc atcatgtctt tttttgtatt
    30241 cttcttgttt ttctgggtaa actttcatgc aaaaagcttt tttcaccatg tcagacacct
    30301 gctttcgtat gaacttcctc caaataacct gcttttggag tgacaccaaa cgcatctgct
    30361 aaatgccaca ggttttcgtc cgtgattgtt tgcaagattg gtccttgcgc tttgacgtac
    30421 gtatagactt ctgctgcctt ttctgctgtc tcgatcaaac caaagacttc atccatgtct
    30481 tttcctgacc cgtagattcc atgttgtggc cataaaacca atcgagtttc tttcattttt
    30541 tcagctgttg cttccccaat ctcatttgtt cccggaacca tccacggaat gatggaaatt
    30601 ccttctggaa aaacaactaa gcattccgta cacatttgcc atagcgtacg actaaaggaa
    30661 cgttcgtcta attcatgcgt aaaactcatc gctaatagat gactagcatg gcaatgcata
    30721 acgatccgat tatctgggtc tacttctaaa cgggcgatat gactcatgaa atgacttggc
    30781 aactcgcttg taggaaggca ttcgttttcc agcccccaca aaagttccag tgttttgcca
    30841 tcttcagcaa ctcgaacgat tccaagattc tcggcaggtt cgctcgctac atttttaaaa
    30901 tatcttcctg aaccagttac aataaaatag catcctgcta aacggctggc atcaaaaatc
    30961 atcgagatgc ggcggatcac tttttcagga tccaagaacg gtgtgatctc ttcttctttt
    31021 agtaaataag aaatattccc gccatttctt tcatcccatc ccaagcgata aagattcgac
    31081 gtcgtttcga tcatttcttt cacataaggc gcttgtaaaa tatctatttt tttcaatata
    31141 gccacactcc tattttctta attccaacac gtctttttga tattcctgga cttcttccag
    31201 ccaatccaaa ccaactggaa cttgattttt gtaacagaaa taattccata catcggcata
    31261 cgggaaatct ttcaattctt ccgtataagc cattcttgaa gtaaagtcaa aagccagctc
    31321 catttctttc aatcgctcaa ctggttccag catagctttc agcagtgctt tttgtgtatt
    31381 tctcgtaccg atgacccaag cagcgatacg gttgatcgtt gcatcaaaga aatccagacc
    31441 gatatttgtt ttttccaaca attcgttacg gactaattct ttagcgattt cctgcaattc
    31501 atcatccatc aaaaccacat ggtctgaatc ccaacgcact ggacgggaaa catgcaacat
    31561 gattcctttg ctgaataaag ctaatgaaga taatttattg gaaatcactt ctgttggatg
    31621 aaaatgtcca gcatctagac aaacaagttt gtcacgagtc aacccgtaac ccatataaaa
    31681 ttcatgggaa ccgactgtat aagcttcagc gcctaaacca aatagtttgc tttctactgc
    31741 atcttgtaca taccgttcat ctaactcttc tgtaaaaatt tcatcgagtg attccattaa
    31801 acgttcccgt ggggtaaaac gatcaatcgg attatctttc atcccatcag gtacccaaaa
    31861 gttattgata cttgtttgcc cagtttcttt cccaaagtac tccgcgattt tgcggcttct
    31921 tttcccgtgt tcgatccagt attcacgaac ttctttatct ggatgagcca atgtaaagcc
    31981 gtctttcatc attggatgcg aaaagaaagt cggattgaag tcaagaccga tttcttcttt
    32041 ttttgcccac tctacccatt tttcaaaatg tttcggctca atttcattta gatcgacaga
    32101 ttcttctgta tccaaataga tagcatgaag gttcaatttg tgtttgcccg gtatcaacga
    32161 ataagccttc tccagatctt gtctaagctc ttcaggtgtt cgtgcggctc ctggaaaatt
    32221 ccctgtcgcc atgattccac cggtcaattc gccatccggt gtcaagaaac ctttgacatc
    32281 atctccttgc cagcaatgca tagaaatttt gatgtcctgt atcttttcta gtacagcatc
    32341 tgtatctaca ccgatagttg catatttttc ttttgcttgt tcgtaatttc tttcaatcgt
    32401 gttcatacga ttactccttt gattttattc acgtaccatt ccatcgtgtt ttgcttattt
    32461 tcatctgggt aaaaagtttc acatggaaac gactgtttga ttacatttct tgcttgttct
    32521 atattcgaaa actgacctgt actgagcatt tgcatggcaa cattcccgat tgctgtggct
    32581 tcactaggtc ctgcttctac aatgattccg gcataatctg ctgtcaattg attcagtaga
    32641 cgattattcg atccgccccc tacgatgtgc agcttttcca atttcgttcc agttaagtgt
    32701 tctactttga tcagctcttg tgcgtaacaa agagcaagat tatcatacac acaacgagcg
    32761 atagctcccg atgagacagg gactggctgc tctgtttcaa aacagtattg ctgaatctct
    32821 tctatcatat ttgggggatt caaaaatcga gggtcattga tttccatcac ttgctgcagc
    32881 ggtttttctt ttgcagcaag ctctgccaat tccgcataac tgtactcgta ctcttgcatt
    32941 cgggcaactt cctggatcaa ccacatcccc atgatgtttt tcaaaaaacg caccgtgtta
    33001 tgggcacccc attcattcgt ataattttca gaaaaagcat tgcgagaaac attggcgatc
    33061 tttgtttcaa tccctaaaag cgaccatgtc ccactgctga tataacccca acgttctcct
    33121 tgacctggcg ttcctaatac agctgacgcc gtatcatgac ttgctactgt gataaaggtc
    33181 acatctggca aatcatattt tgaaaattgt tctttttgca gatctcctag tatcgttccc
    33241 ggctctgtca atggtggaaa taattccttc gagacaccga ttttttcaag aagttctatt
    33301 tcccattctt ttgtccctgc attcagcagc tgcatagtag atgcattagt tttctccgtc
    33361 accatgttac cagtaaagac ataccctaaa taatcaggta tcagtaacaa tttatgtgct
    33421 tttgccaaat tttctttttc ttcagcaaac aattggaaca aggtattgaa aggctggatc
    33481 tgaatccctg tctgctcata caacttatct agagaataga tttcagaaaa ctgttctact
    33541 gctccattgg tacgcttgtc ccgataagca atcggatcat ttatccgatc cccttgttca
    33601 tcaatcagac agtaatcgac tgcccatgta tcgatccccg caaaaaggcg atcaatccct
    33661 gtctgcttta atttttcaaa tccaatcaaa atctctttga taagataatc gatcttccag
    33721 cgatcgtgac cgtctttgtt ccgaaagccg tttttgaacc ggtgtacttc ttccaagatc
    33781 aaagaacctt ttgcatccct cgtacttttc attagtcgtc cgcttgatgc accgatatcg
    33841 atggcaacaa atgttggatt catttctcca cctcttttac tttattgttg attagcactt
    33901 tattaataaa gcgttatcat ttattgacaa tgctatcata atacagaatc ttatattttg
    33961 cttcacacta ttttggtata taattgcatt atcttgacaa gttgggagtg tttacatgga
    34021 ttactgggaa aaagccaacc actcatggtc ggaagactca aaaagagtga tcttgacgcc
    34081 tacacaaaga agcaggaatt tattttttta tatccaagaa atcggttact tcaaagcacc
    34141 aatcccttac tatactgaac gagcacattt accttcttac ttgataaagt ttactttgtc
    34201 tggcaaaggt tcgcttacat ataatgataa tcagcaacag ctaaaagctg gagacttatt
    34261 ttttattgac tgccaagaat atcagcgtta tcaaacgatc agcgaagaac catgggaaat
    34321 ggattggatc catttgaatg gtcagaacgt ccaatcattt tatcaggagt tcacaaaaga
    34381 cggccataac accttccatt caacaggaga tccatcagaa aacaagatcc accatttgat
    34441 cacagaattg atccacatgc aacaaggcac gaatgcccgt accgatttcc agtcctctgt
    34501 agctatccac gagttgctga atgaactgat tttgcaaaag tatcagctag atttcgaaga
    34561 agaggatatt cccgcctacg tttacaaact gaagcaatac ttagacgaac atttcaaaga
    34621 aacgattaca cttgatcaat tagaacaaca attcttattg aataaatatc agctgagcaa
    34681 agatttttcc aaatttatcg gttctccacc aatcgattat ttgatcagca aaaaactttc
    34741 ttacgctaaa gaccttcttc ggtatacaga atttacggtt cgaatgattt ccgaagaaat
    34801 agggattgag aattttgctt actttagccg cctgttcaaa gcaaaaacag gactatcgcc
    34861 gagagacttt cgtaaattca gctgattcat ataatatgta cgtcattttt tcaaaatatt
    34921 ttgaaatata tagataaaaa taaaataacc gcagtttctg cttctatgta aacaacagca
    34981 gctactgcgg ttatttttta gtgatcattc tcattgttca tcttcaaaat gttcataatg
    35041 aaccaactgt ttctgtgtat cacgtttcaa aaattcttta ggcgttatgc cgaactctct
    35101 cttgaatgct ttcagaaaat gcggataaga agaaaaacca cttttactat atacagcact
    35161 agagctgcgg tattctcgca acaagtgctt ggcatagatc agctttttct tcattacata
    35221 ctgatgaaac gtaaatccag tgtacttttt aaattctcgt gtgatatggt atttgctgac
    35281 aaaaaactgc ttttccattt cctctaaaga aagcggatga gataaatttt ttgagatata
    35341 ttgtaacatt tgttccacac gctcattagg aactacagca ttctgttctt gcgcactctc
    35401 ttctttttgt acaacttgat tgagaaaaat caagtattct accagagctt gttcatattt
    35461 tatgtcttca ccgtaaattt ttttatcagg aggatggtcg ataaatgata atttttgctt
    35521 caatacttcc tgttctattt gcaatacacg actcctcttc tctcccattg gttgaaaaca
    35581 agccgataga ttagaccatg gcgtggaacg actttctaga aaagaagggg tgacaaagat
    35641 atagacacgc tcgaatacat ctgaagattg gcggacgata cgatgcaaat cattggaatg
    35701 gatgagcagc acacttcctg ctttgatcgt aaattgtcgt ccgttcaaat aaaaagtcgc
    35761 ttctcccttt aatgtagcat ggatctcata gaaatcatga tgatgataga tcgttttatt
    35821 ttggctgacg acatctttga catgatagat ttcatactga tcagaaacca tcgtattgac
    35881 ttgttcaccg ctgattttct tttggagcat gtgccgcacc tccttttgtt cacttatgca
    35941 caattttatt ataccacgtt ttttgtaatc aaaaagagct gcttttatat actcttataa
    36001 aaaagaagag acgtggcatc ttctagcccc gccccctcca ttctaaatat tagttaacca
    36061 acgtcttctt tacattattc aagaagactt cttccataac gaatcccatc atcttttgtt
    36121 tcttcaagaa ccacatacag gtacggacga ttcttcatga tgatttcttt gatttttgtg
    36181 tactcgatca caccgtctcc caaatttacc ggcacgattt tttcctgttc gacacgaaaa
    36241 tctttcaaat gaaccgcaca tatcttttca ccaaaacgtt caaaagcttc ttctaccaat
    36301 tggacttgtt cttgataatc actacttgtg atcaaattgg tcggatctaa aatgattcct
    36361 agatagtccg aatctactgc ttggatcagc tgctctaccc ttgctaaaga atacaccgga
    36421 tgattcaaac ctggttctat ccccaccatc atttggtatt tttctccagc ttttaccaag
    36481 cgacggataa cggacacagc ttcagcaaat gcttcatctg tgaaattttc ttccgtatat
    36541 tggatttccg gcaaaacaca tccagtttcc gaagcaacca tcgacgcacc aaaggaagaa
    36601 gcatagcgaa gatactgctc gaactttgtc agcaactctt gtcgtatcgt taaatctgga
    36661 tgaatcatat tgatatagca acttaaaatt cctacggtca cttcttgctt ttctaaaact
    36721 cgtttgacat aatttcccat cccagaattg atctcatttg cgccagaagg tatttcagga
    36781 aacgaaagac ctaaggcgag ctgcagtgta tgaatccctt gttcttttgt tttttctgca
    36841 agatcttcta tcgtttggac ttcggaaaga tcatgtcctc taattgtaat gttcaacata
    36901 actattctcc taactcagca tattgggtag ccacatgacg atttgcggga agtaagtaat
    36961 caaaaacaag acgactaaga taaccccata aaaaggaaga agcggtttca gcattgcctc
    37021 tgcttttacg cctcctacac ttgcaccgac atatagtcct gtacctactg gcggcgtgat
    37081 ggagcctaca cacagattca tgattgaaaa caacccgtaa tgtacaggat caacccctac
    37141 acttgtgacg attggtaaaa atattggtgt aaaaatcaag atagcaggcg ccacatccat
    37201 aaacataccc accagtaata acacaagatt gactaataac agaataataa atttattatc
    37261 cgttacgccc ataatcaacg aactaattgc ctgaggaata ccggtaaacg ccatggcgaa
    37321 agacatcatg gaagaagtag ccaataagaa catgatcgtc cctgacattg ctactgattg
    37381 aaacaacata ttaggtatat cttttagttt taccgtttta tagtaaaaca tagcaatcaa
    37441 cagggagtaa atgacagcta tcgctgaagc ttctactgct gtaaatatcc ccgtcaagat
    37501 accaccgata atgatgacaa tcaatagaag gctaggaatc gcctctttta ttatttttcc
    37561 agcatgagcc atctgtcctt tttcgacaac agggtattta ttctttttag caacgatgaa
    37621 tgctactagc atgattccta gcgcccacaa agcgcctacg agataacctc ccataaacaa
    37681 agcagagatc gaagcaccac cagcaacaag tgaatacatg ataaaagcag tacttggagg
    37741 aatgaccata cctgtcggag ctgacgcgat attgaccgct gtcgcaaact tccggtcata
    37801 tccttcttcc acttgctgag ggatcaacac tccgccaatc gcggtagaag cggcaatggc
    37861 cgagctagag atggaaccaa ataaagcatt ccctaacaca ttcgtatgag ccaaagcccc
    37921 cggcacgcgc ccgacgaaca acatcgcaaa atccactagt ttcttagcta tccctccatt
    37981 attcatgata atgcccgaga gaacgaaaaa cggaatagcg atcagcgaaa aactattgat
    38041 acttcccacc attttttgag ctgaactaaa agcagaaaca tcaaacggta taactaacaa
    38101 taaagtcatc atcgatgaga atgcaatact tatagcaatc ggcatcccta atacaagcaa
    38161 cacaacaaat acaaaaaata acactaatcc ggcttctatt gccattttat tcctccattt
    38221 ttatattttt tcttgaattg ctggcttgtt gcttaatgta tcgattagat tgatcaggct
    38281 atagataatg atgagtaccc ccgatacagg tactgacaga taaacatatc ccataggaat
    38341 acttaaagaa ggcgagatct gccccattgt caatgtgaca gcattccatc cgccatacac
    38401 catgatcatc gcagcaaata ccagaaataa actttgaaca acaacttcta aaattttact
    38461 ttttttgcca gttaattttt cacttaaaac cgttacggct aaatgtgctt tttgtcccac
    38521 aacatacgcg gaagacaaca tggcaaacca gacaagaccg aatcgaacaa gctcttccgt
    38581 gaccgtattt ggatttccta aaaccgttct ggaaaatact tgccataaaa caacagcgac
    38641 catcccaaca agcaaaacag ctcccaggat ctctaatacc ttgtttaaaa tcattttcat
    38701 cacagctacg ctccttgctc ttggatcttt tgataaaggt ctttgtaggt aggatcgttt
    38761 ttgaatatat catgaagtgg ggcaactgcc tctcgaaacg gtgtattatc tacatcattg
    38821 aattggactc catgttcttc ttgtgctaaa gctttttctt cttcaattgc ttttgcaaaa
    38881 gcttcaattt caaaaaccgt tgattctttc gctgcttctt caatggcttt ttcctgctca
    38941 tcagttagct gttctttgat ggcatcattc atgatgatga tatctggcac acgtgtatgc
    39001 tcatcatagg aatagtattt agccacacca ccatgtccag ctgtatacaa gacaaattca
    39061 ttgttttctg atccgttgat caaattggat tgaagagacg tgtacacttc atcacttccc
    39121 ataggtacgg gcgaaccgcc taaaagctct accattttga tagcggtttc gctttgcatc
    39181 acacggatct tctttccctt caaatcttcc ggcgtatgga tcggaccgtc taccatataa
    39241 aagctgcgct gaccagaatc ataataggtc aatccggtaa atcctaaatt tttggtcgat
    39301 tggtagattg gctccattat ttcttcatta tccatcacct taaagaaatg ttcttcatca
    39361 ttgaatagat aaggaactga aaaaatcgaa taatctttag agaaactttc caacgctgag
    39421 gcacttactt ttgtgaaatc tactgctcct gactgtgtca attcgatcag ttcacgttct
    39481 ccacccagtt gtccatctgg gaaatactgg atctgtacgg aaccatctgt tttttctttt
    39541 actaaacgcc caaattcatt cattgcatca attactggct ggctgttgct tgcataagca
    39601 aagcgcaagg taactatctc atttacatct gcatctgcga cagttgccac actagaatta
    39661 tttccgcagc cgcttagacc tagcagacct aggctaagcg aacttgctgc aaacagcagt
    39721 gatgtttttt tcatatgggt ttcctttctt ttaacaataa gagacgagca attctgtcaa
    39781 acaaagcacc gttaacgact gtccataagg cattgctgtg attccgatat tccgataaaa
    39841 atctaaactg tcccccattc ctgtcccgat tgaaacgttt tgaacttctc ctttttcatc
    39901 gatttgttca agcaatcctt ggatagcacg ataagcgact tctttatatt cttgtggtaa
    39961 ataacgttta tggaccgctt tcaaaatacc gtaagcaaat cctgcagttg cagaagattc
    40021 tacgtaagaa gaaggatcat ccaacaacgt atgccataaa ccgctttcag actgataagt
    40081 ttttaatgca cgcacttgtg cttcaagtgt ttcgatcaat aatttgcgca atgcatcttc
    40141 tttcgataat tctaagattt caatgatctc tggaatagca atcgtcaacc agcagtttcc
    40201 acgagcccaa aatgcttcgg catagttatg atttccttca aacgtccagc cgtgatacca
    40261 caatcctgtt cgtttatcca tcaagtattt gatatggatc ataaactggt aacgagcttc
    40321 ttctaaatat tctgggcgat tcaacagttt cccgattttt gccaaaggaa gcactgtcat
    40381 catcaatgta tcgtcccaca actgattctt attttccggt ccatatgtgg catgctgtaa
    40441 cccgtcttca tttgtccgag gcatgtcatt catgacccat tctgcccatt gttctaaata
    40501 aggcaaatag cgtgaatctt tcgtgtcttc atacaagtaa gccatagtca atagcggtgc
    40561 cattgtattg acgtttttgg gcggagcgcc ttcttccata cgatcctcaa accattcatt
    40621 gaccacttga taagcgcgag gattttttgt catctggtaa tttttataaa taccatatag
    40681 accgacacct tgtggccaat tccacactgt ccagcttttg tcatctactt tcagtccatc
    40741 aaagtccaac aaaaattcac cattatcgtc tttgatcgtt gtcaggtttt caacgagcag
    40801 gtcaattttt tctttcacta attctttagg tacaaaaatc tcttctttaa taatcatctt
    40861 tacgccacct ttttaaacgc tttcatttta tatccttaat atagcagtaa taccgtccac
    40921 tataaatatc gctttttgaa caaaaactgt ctaaaattga cctaaatgtt aaataacaaa
    40981 agtaactttc agttacttgg aaattaaaaa aagaacttgg agaaacaacg ttttccaagt
    41041 tctttttcat ctattttctt atcttttttc tacgctttct tttgcttatc cgttgtaagc
    41101 atcaatgatg atctgtttca attcagagat caatggttct ttcggattag ctgtcgtaca
    41161 ttggtcttcg taagctaatt cagccatacg gtctacggtt gtatctagtg tttcttgtgt
    41221 gactccttga gcttttagac tcatgttgat gccgacagat tttcctaatt cataaacagc
    41281 cgttgctaaa gcttcgacta attcctctgt tgtttcgcct ttcagaccta agaattttgc
    41341 gatatctgcg taatctgtat ctgcacggaa atagtcatat ttagggaaca tcgcatgttt
    41401 ttgtggatct tttgcattat agcggataat atgcggtaaa agaatcgcat ttgtccgtcc
    41461 atgagggatc ccgtattccc cgccgatttt atgtgcgact gagtgacaaa tacctaggaa
    41521 tgcgttagca aacgccatac cagccattgt agaagcgttg tgcatttttt cacgtgattc
    41581 catatcaggt gtttttactg atttttctaa atagtcaaag actaacttga ttgcttgtag
    41641 gctcaatcca cgtgtataat ctgaagccat gactgaaaca taggattcga ttgcatgtgt
    41701 taacacatcc atcccggtat ctgctgtgac agatgctgga actgacatga cgaattgagg
    41761 atcgacgatt gcgacatctg gcgtcaatgc ataatctgct aacggatatt tcacatgtgt
    41821 ttcactgtca gtgatgaccg caaatggcgt tacttcagaa ccagtccctg atgttgtcgg
    41881 gatacatacg aattgtgttt tttctggttt gtcgatctta tatgtccgtt tacggatatc
    41941 caagaatttt tgttttgcac caaagaaaga tgtgtctggg tgttcataga acatccacat
    42001 tcccttagct gcgtccattg cagaaccgcc tccaagagcg attactgtat ctggcttgaa
    42061 gtctgccatc agtttcgttc ctgcgtatac ggtatttgtc gatgggttcg gttcaacatc
    42121 tgagaagact tcgatcttca catcattttt acgtcgtgcc aatacttcac gaactgtatc
    42181 gcagtaaccg aattggacca taccagggtc acaaacgatc atgacacgtt caacgttttc
    42241 cattttctct aagtacaata gagagttctt ttcaaagaag atttttggcg gtaatttgaa
    42301 ccattgcatg ttatttctcc gtttcgctac agttttgaca ttgattaagt ttacagcaga
    42361 gacgttacga gaaacagagt tcttaccata agaaccgcat cccaaagtca atgacggaat
    42421 catttcgtta tagatatcac cgatgccgcc ttctgcagat ggtgtattca ctaaaatacg
    42481 acaagctttc attcgaagac cgaatttgac atgcaactct tcgttttctg aatggataac
    42541 tgctgtatgt cctaatcctc caagattcaa cataccttca cacaaatcaa acgcatggtc
    42601 cgtgttgtta gctttgatca tggcaagaac tggagaaagt ttttctcttg atagcggata
    42661 atctgggcct acgccttcca attcagcaat cagcattttc gttccttttg gtacactgat
    42721 tcctgccaat tttgcgattt ccatcgcaga atgtccaacg attgatggat tgaccgcata
    42781 ttttccttcg ttcatcactg catcttctaa tttttgcaat tcatctggtt ttacgatata
    42841 aacttggtgt gcttggaatt ctgcttttac tgctgcatag atttctttat ccacgatcac
    42901 agcttgttca gaagcacaaa tcattccatt atcaaatgtt ttcgaaacga tcaaatcgtt
    42961 cactgcacgt ttaatttttg ctgttttttc aatataagct ggtacgttac ctggacctac
    43021 acctaaagct ggttttcctg tagagtaagc agacttgacc attccagctc cacctgtcgc
    43081 taagacgatc gcgatacctg gatggttcat tagcatagaa gtcgcttcga tcgaaggatg
    43141 ttcgatccat tggatacaat tttcaggagc tccggcagcg atagcagcat cacgtaccac
    43201 tcgtgctgct tctgcagaag atttttgggc acttggatgg aaagcaaaaa cgattggatt
    43261 tcttgttttc aacgcgatca ttgatttgaa aatcgttgtg gaagtcggat tcgttgttgg
    43321 agttactccg caaaccacac cgactggttc agcaatttca atcaaccctt tttggacatc
    43381 ttcattgatc acaccaactg ttttatcatg tttgatatta tgccaaatat attcggacgc
    43441 atacatattt ttgattgctt tatcttcgta aacaccgcgg cctgtttcct ctacagccat
    43501 cttcgctaat ggcatatgtt gatccaaagc tgccattgcc atttcatgca cgatatggtc
    43561 tactttttct tgatcaaagc tttccatttc tttcaatgct gcatttgctt tttctgccaa
    43621 atcatcaatc attgcttgta cgtcaattgt cttcgttttt tctttttcta ttactttagc
    43681 catcattatc ctcctaatgg gtcatggtta tttgttaaat aattcacatg tatatcatac
    43741 aatatttatt tttctatgta aacacttttt tgtgattttt ttcacagtta tttttaaata
    43801 taaaattgct ttatttacag gtgtttgtat agaaaaatat ataaagttat ttattattat
    43861 ttatatgaca cacccgatta ttcaatattg agcaatatat cccatgctct tatctctttc
    43921 ttttttgaat aaaaaatgag gtaggacatc tttctgccct acctcgtctt taaataagct
    43981 ttgatactta tttttcttcc ttctcttctg tctcttccag cgtttctgtt gtttcagttt
    44041 ttacttcaac tgtctcagct ggttcttctg ttgtttcaac agtagtcgtt gcatcatttg
    44101 ttacgacagt ccctggtttg accgttttga ttgctgcacg atcgtactca aggaagatcc
    44161 cttcgcagtc taaaacaact gtccgtttgt cattgtcgat ttctgaaaca actccatgta
    44221 atccgccgat cgttacgact gaatcacctg gtttcatgct atctaacaag ttttgacgtt
    44281 cttgctgttg tttcttttgc gaacgtgaca taaaaaacca cattgctaat agcgcgataa
    44341 acatcacaag cattggtaat gttcccattt ttttcacctc actaattctg tatctacaaa
    44401 aaacacttta gcagaaaaat gctgataaca ctagtttttt acttaatttt taaaagattt
    44461 tcctttagaa gctttttgcg ttttctttat tgaacccgta ttcttcaaaa aaggcttctc
    44521 taaattcaag caaattatca tccatgattg cttgacgtac ttgtttcatt agattcaaca
    44581 agaagtataa attgtggtaa gaagtcaaac gaatcccaaa tgtttcatca catttgatca
    44641 aatggcggat ataggcacgt gtatagtttt tacatgtata acaatcacat ttctcatcaa
    44701 ttggacggaa atcatgggca tatttcgcat ttttcactac tagacggcct tgagaagtca
    44761 tacatgtgcc atttcgagcg attcgagtag gcaagacaca gtcaaacatg tctactccac
    44821 ggataacacc atcgatcaat gaatcagcag caccgacacc catcaagtaa cgtggtttgt
    44881 tttcggggat caacggtgta gtgaattcta atacacgatt catttctgat ttaggttccc
    44941 cgacagataa cccgccgatt gagtaaccag ggaaatccat cccgactaga tctttcgcgc
    45001 tttgacgacg aagatcttca aaccctgctc cttggatgat tccaaataat ccttgacggt
    45061 ctggattagc atgagctttc aatcctcgtt ctgcccatcg agacgtacgt tctactgatt
    45121 ttttcacgta gtcgtaactt tcatcgaatg gtggacattc gtcaaaactc atcatgatat
    45181 cagagcccaa tttattttgg atgttgattg ctttttctgg tgataaaaac atacgcgaac
    45241 cgttcaagtg atttttgaaa tgcacgcctt cttcttcgat attccgcata tcacttaatg
    45301 aaaaaacttg aaagccgcct gaatctgtta aaatcggttg atcccagttc ataaatttat
    45361 gcagtccgcc agcttcttct actaagtctt caccagggcg tagccataaa tgatatgtat
    45421 tacttaaaat aacgccagca cccatttctt tcaactcttc tggggacatt gtttttactg
    45481 tagctaatgt tcctactggc atgaacattg gtgtggggaa agttccgtgt ggcgtgatca
    45541 attcccctaa acgcgcgcca gtatgttttt ctttcttgat taaacgatag cgaatggcag
    45601 gttgcgtcat tcatttccct actttctata taaaatatct tgacaactcc caccatcata
    45661 atcgtttcaa gtgctttttg caagttccct cggcttcctt ctactttatt taatattctt
    45721 ttttttatcc tttttttatg aataacaaaa aaaagaaaaa cattatgaca gaaatatttt
    45781 ttgatacatg gtatattggt gttattaaag aaaaggaggg aaaaatcatg caatcttctg
    45841 cagaattaaa acgccaagca aaagagagtc taaaaggacg ttggggacaa gctgttttac
    45901 taaatctaat ccctacattg attggtattg ctactattct tcttattatg attcctatta
    45961 tcttgtttgc caccaccata tatgatggat cagcgagcgt ctcagaagcg acaacttcag
    46021 gaggcggtag ttcaggaggc ggtggcggaa ttatttccag catcattagc gctttgttca
    46081 tgagcggtat ttcttggact tatcttgata ttttacgtgg acaaaaagat cagatcgaac
    46141 cattcaaaga tgcatttcgt ggattcaaag gtttgtttat cggcggagtg atcagcttag
    46201 ctgtgctgat ctcgatcttt acaacattat ggggtttgct attagttatt cctggaatca
    46261 ttaaaagcta ttcttattct caaagctatt ttatttacta cgatatcgtc caagaaacag
    46321 gacaaacacc taaagtactg gatacgatca ctgcaagtcg aaaattgatg gatggttata
    46381 aaggacaact tttctggtta gatctttcat ttatcggctg gcatatctta gcattagcaa
    46441 ctgctggtat tggttatcta tggctcagcc catacatttc tgcaacaaaa gcagcatttt
    46501 atgaacaatt gccaaaagat atttaaaaga gagtgtgact tgtgaccgag ggggtggcag
    46561 gagaacaccg tttatcgaga ggcagtaaaa gaaacattca gcccattagc aaaaaggagg
    46621 tgtgacaaca ttcatgtcac acctcctttt tgtatagttc cagtaaaaag aaaatcaact
    46681 tagaaacagt taatcattcc tcttcttctg cttccggtaa tcgatggaac ttgatcagtt
    46741 cattcaccgc gattggtacg atagataaaa tccctaccaa gaaccagtga ttcaaactga
    46801 tgtgtactag gccgaatagt tcttgcgtaa atggcagcaa agcaacagcc gttgtaatag
    46861 ctaatgccag taacgccatt tctaccaaag ctttattggt cgctaaattg actttgaaga
    46921 tcgattggct gctgcgtaca ttgaacacat gcagaatcga tgaataagct aagatcaaga
    46981 atgcaacagt ttgaccgaca tgctgacttg cgtctaccca tgggctgata gtatcgacat
    47041 aactacctaa ataataacca aacaaggtta cgatggtgaa gacccctgca tttatggcaa
    47101 ttttttgcaa taaacctcga gcaaagatcc cttcattttt cggaatgggt ggctgatcca
    47161 tgatgccgtg ttccgctggc tctcttccta aagcaaatcc tggaagtcca tcagcaacta
    47221 cattgacaaa cagtagttgc acagctgtaa atggtgctcc ccatcctaac agcatcgcaa
    47281 tcaaaacaat aaagatctcc gaaatattac agcttagcaa aaagttgata gctttacgaa
    47341 tattctgata aaccgaacgt ccttgactga cagcgtctac gatcgtcgca aagttgtcat
    47401 ccgtcaaaat catatctgcg gcactttgcg ccacatctgt tcctgtgatc cccatggcac
    47461 acccaacgtt acttgctttc aacgctggtg catcattcac accatcccca gtcatcgcta
    47521 cgactgcacc cgtacgttgc caagatttga cgatccggat cttgtcttct ggcgttaccc
    47581 gagcatagac agagagagct ttgacacgcg catctaattc ttcgtctgat aattgatgca
    47641 gttctgatcc tgaaagtgct tctgacggat cattcaagat acctaattct ttcgctatgg
    47701 cactggcagt caccacatga tctccagtga tcatgactgt tttgatgcca gcctttttcg
    47761 ctcgcttgat tgctccttta ctttctggac gtggtggatc gatcattccg atcagaccaa
    47821 tcaaacgtaa gtctttttcc agtgcttcag aagaaatatc ttgcggttct tcgtcataag
    47881 tagcataacc aacagcaatc actctcagtg cccgtttacc aaagcgatca ttaacgatcg
    47941 ccgcttgttc tacatcgcct gaactaaagc gcggagcaag aacatcgaat gctcctttgg
    48001 tgattgagat gtatttttcg cccatttggt gaactgttgt catcattttt cgttcagaat
    48061 caaaaggaag ctcgtttact cgtggatatt tttcttctaa ttctgccttt gtatgataat
    48121 tctcttcaac agcccttacg atggctactt ctgttggatt acctttgata acaaggtcgt
    48181 catcttcttg ctcgaccgtc acatctgtac atagtgcagc cattttcaat atttccatcg
    48241 cgctgttcgt cattgaatct tcgatattcg ttacttcatc ttctttgctc catacccggc
    48301 ggacgcgcat ttcattttgt gtcagcgttc ctgttttgtc tgagcagata acattagcgg
    48361 ttcctagcgt ttcaactgct ggcaactgtc ggatgatcgc atgtttcctc gccatcttct
    48421 gcacaccgta tgcaagtgtc aacgtaacaa taacagtcaa ggtttccggt acagctgcaa
    48481 cagccaagga aatcgaagtc atgaacatct caaggagtgg ttctccttgc agttcaccaa
    48541 tcacaaatac taaagccgca gccgctaaag cgatcatact gattcgtttg cctagctgat
    48601 tcaatcggac ctgtaaaggt gttttttgca tagtattttc gttcaaaaga tccgcgatct
    48661 tacccatttc tgtagccatg ccaatggcag tgacgatcgc tttcccacga cctgcagcaa
    48721 ccgtacatcc tttaaatacc atattaagtt ggtcacctaa tgactcatct tcttcagcaa
    48781 taaatgctgc atctttttca actgcttcac tttctccagt caacgccgat tcttctacac
    48841 gtaattgtga tgcttcgatc aaacgggcat cggcagaaat agcatcaccg gactctaaaa
    48901 caagaatatc acccactacc agttgctctg actcaacagt ggtcactttg ccgtctcgta
    48961 tgacagttgc cgtttgttta ttcatatctt gcaaggattc cagtgctttt tctgcgtttc
    49021 cttcttggac aatcgccaat accgagttga tcacaacgat agcgatgatc agcagtcctt
    49081 caaataaatc cccatgttct gtagcaaatg cggtataaaa ggaaatcacc gctgctacaa
    49141 gaaggatgat cgttgtaaaa tcggacaagc ttcgaaagaa ttttttgatc atcgattctt
    49201 tcggcgcctc atcgaattta ttcaatccct gcttttttcg ccgttcatcc acttcttgtg
    49261 aagacaaccc agtatctgga tctgcttctg ttaccttctt tacttcttct attgtctgct
    49321 tgtattcttc catcctattc ctcctttaaa atcttcgtac aaaaaaaggt acttatttta
    49381 ctataacatc ctttttttat ttacttcaaa taaagggctt atcgattatt tgttaagaaa
    49441 gcgtaaaatt taaagtgctt atatttgcaa acgctaccaa aggatgctat tatatagatg
    49501 taaggaagga ggataccatc aaatagacag aagaatttct cgatagaaca gtgtaagcaa
    49561 acaaagttgt tagaagtaca ggtttcaccc agacatgcgt gtagagtaca aacctataaa
    49621 tagtgaatgc gtatggatgt ttggatcacg aagtatccga aacaagaagt aatgcgatac
    49681 gtaacaattg aataacgtaa acgtctgaaa attgaatagg aacaagagat gtttcaaatg
    49741 ttgaagatgt aacgaaaaca aaatttttgt ttaacatgaa gaaattcaac accgtgcttt
    49801 gcatgtatga tggaaagtta caatcatttc tgtttatttg aatccttccg aggtacaaga
    49861 aagccgagct tatcgagagc tcggcttttt ctttgtcata aaatctttgt aaagcttttt
    49921 tgttattcaa aactagcggc ttttcataac cttatttcac gaacatcgca tctccaaaac
    49981 taaagaaacg atagcgctct tcaatcgcat gctggtaagc agataaagtc aattctcttc
    50041 ctgcaaaggc actaactaac atgactaaag tcgattttgg caaatggaaa ttagttgaga
    50101 aagcttctac cactttgaat tcatagccag gcgtaatgaa aatatcagtc catccgctgt
    50161 ctgctttgat ttctccatta aatttcgttc caatcgtttc taatgttcga attgaagttg
    50221 ttccgacagc cacgattttc ccgccatttt tacgtacttg gttcaatcgc tcagccgctt
    50281 cttccgtcaa acggtagaat tcactatgca tatggtgttc ttcaatttta tctacgctga
    50341 caggtcggaa tgtacctaac cctacatgta gtgtcagata gactaattca acaccctttg
    50401 cttcgatctc atcaagtaat tctttcgtaa aatgcaatcc agctgttggc gctgcagcag
    50461 aaccattttc tttcgcatag acagtttgat agcgatctgg gtcttccaga cgttctttga
    50521 tatatggagg caatggcatt tctcccaatg attcaagaat ctctagaaaa attccttcat
    50581 actcaaagtc aatgatccgt ccgccgtgtt ccaattcttc tttcactact gcttgcaatc
    50641 tgccatcacc aaaagagatt cttgtcccaa ctttggcacg tttcgcaggt ttgatcaatg
    50701 tctcccacgt atctccttgt gtattcgtta gaagaaggac ttctaaatgc gcaccagtat
    50761 ctggtttttc tccatataaa cgagcaggca gaactcgtgt attattcata actagcgcat
    50821 caccaggctg taactcttca atgatttcat ggaaatgttt atcttccatc tctccagttt
    50881 tccgatccaa aacaagtaaa cgagaatgat cgcggtcttt taatggtgtt tgtgcgatta
    50941 attcttccgg caagtcaaaa tcaaaatctt ctgttgttaa catatcgtca ctcactttct
    51001 atcatgtgct tcatcattct atcatgtttt tcgttctgtt ggcaaaatca gcactttttc
    51061 acttctattt tttgttttcc taaataaaaa aactacccta tttgtatttt ttcattgttt
    51121 taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg gcaccatgat
    51181 tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag aggacgtcaa
    51241 tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa gcggaaaaac
    51301 gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa ttttgattga
    51361 tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg gctatgttat
    51421 ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc ttgtccaaaa
    51481 attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga ttgatcttgt
    51541 ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg gtcagcagca
    51601 gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga tggacgagcc
    51661 attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca aagatttaca
    51721 agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag cgattaaact
    51781 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
    51841 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
    51901 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
    51961 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
    52021 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
    52081 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
    52141 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
    52201 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
    52261 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
    52321 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
    52381 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
    52441 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
    52501 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
    52561 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
    52621 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
    52681 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
    52741 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
    52801 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
    52861 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
    52921 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
    52981 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
    53041 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
    53101 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
    53161 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
    53221 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
    53281 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
    53341 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatagttt tgaaaatacg
    53401 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
    53461 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
    53521 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
    53581 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
    53641 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
    53701 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
    53761 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
    53821 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
    53881 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
    53941 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
    54001 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
    54061 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
    54121 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
    54181 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
    54241 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
    54301 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
    54361 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
    54421 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
    54481 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
    54541 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
    54601 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
    54661 tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat
    54721 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
    54781 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
    54841 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
    54901 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
    54961 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
    55021 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
    55081 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
    55141 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
    55201 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
    55261 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
    55321 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
    55381 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
    55441 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
    55501 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
    55561 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
    55621 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
    55681 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
    55741 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
    55801 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
    55861 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
    55921 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
    55981 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
    56041 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
    56101 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
    56161 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
    56221 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
    56281 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
    56341 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
    56401 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
    56461 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
    56521 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
    56581 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
    56641 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
    56701 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
    56761 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
    56821 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
    56881 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
    56941 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
    57001 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
    57061 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
    57121 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
    57181 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
    57241 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
    57301 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
    57361 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
    57421 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
    57481 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
    57541 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
    57601 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
    57661 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
    57721 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
    57781 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
    57841 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
    57901 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
    57961 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
    58021 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
    58081 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
    58141 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
    58201 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
    58261 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
    58321 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
    58381 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
    58441 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
    58501 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
    58561 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
    58621 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
    58681 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
    58741 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
    58801 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
    58861 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
    58921 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
    58981 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
    59041 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
    59101 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
    59161 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
    59221 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
    59281 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
    59341 gcggtaataa gcccaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
    59401 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
    59461 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
    59521 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
    59581 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
    59641 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
    59701 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
    59761 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
    59821 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
    59881 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
    59941 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
    60001 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
    60061 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
    60121 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
    60181 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
    60241 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
    60301 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
    60361 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
    60421 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
    60481 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
    60541 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
    60601 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
    60661 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
    60721 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
    60781 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
    60841 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
    60901 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
    60961 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
    61021 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
    61081 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
    61141 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
    61201 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
    61261 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
    61321 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
    61381 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
    61441 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
    61501 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
    61561 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
    61621 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
    61681 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
    61741 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    61801 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    61861 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    61921 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    61981 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    62041 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    62101 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    62161 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    62221 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    62281 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    62341 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    62401 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    62461 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    62521 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    62581 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    62641 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    62701 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    62761 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    62821 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    62881 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    62941 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    63001 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    63061 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    63121 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    63181 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    63241 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    63301 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    63361 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    63421 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    63481 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    63541 ttaagtttac gtttatgcct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    63601 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    63661 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    63721 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    63781 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    63841 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    63901 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    63961 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    64021 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    64081 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    64141 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    64201 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    64261 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    64321 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    64381 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    64441 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    64501 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    64561 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    64621 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    64681 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    64741 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    64801 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    64861 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    64921 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    64981 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    65041 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    65101 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    65161 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    65221 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    65281 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    65341 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    65401 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    65461 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    65521 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    65581 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    65641 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    65701 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    65761 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    65821 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    65881 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    65941 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    66001 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    66061 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    66121 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    66181 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    66241 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    66301 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    66361 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    66421 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    66481 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    66541 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    66601 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    66661 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    66721 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    66781 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    66841 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    66901 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    66961 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    67021 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    67081 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    67141 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    67201 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    67261 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    67321 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    67381 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    67441 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    67501 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    67561 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    67621 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    67681 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    67741 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    67801 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    67861 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    67921 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    67981 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    68041 ataatactat ctgttgcttc aagattttgt ttgatagcat caatcaattt attcttttct
    68101 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    68161 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    68221 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    68281 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    68341 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    68401 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    68461 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    68521 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    68581 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    68641 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    68701 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    68761 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    68821 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    68881 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    68941 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    69001 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    69061 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    69121 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    69181 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    69241 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    69301 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    69361 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    69421 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    69481 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    69541 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    69601 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    69661 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    69721 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    69781 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    69841 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    69901 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    69961 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    70021 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    70081 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    70141 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    70201 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    70261 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    70321 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    70381 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    70441 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    70501 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    70561 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    70621 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    70681 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    70741 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    70801 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    70861 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    70921 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    70981 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    71041 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    71101 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    71161 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    71221 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    71281 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    71341 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    71401 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    71461 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    71521 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    71581 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    71641 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    71701 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    71761 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    71821 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    71881 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    71941 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    72001 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    72061 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    72121 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    72181 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    72241 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    72301 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    72361 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    72421 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    72481 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    72541 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    72601 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    72661 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    72721 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    72781 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    72841 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    72901 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    72961 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    73021 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    73081 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    73141 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    73201 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    73261 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    73321 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    73381 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    73441 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    73501 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    73561 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    73621 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    73681 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    73741 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    73801 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    73861 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    73921 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    73981 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    74041 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    74101 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    74161 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    74221 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    74281 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    74341 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    74401 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    74461 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    74521 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    74581 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    74641 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    74701 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    74761 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    74821 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    74881 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    74941 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    75001 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    75061 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    75121 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    75181 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    75241 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    75301 ggcacaaaaa cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    75361 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    75421 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca cggtttcaag
    75481 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    75541 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    75601 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    75661 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    75721 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    75781 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    75841 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    75901 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    75961 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    76021 atcttttttc atttattaaa actgcctcct tatgaactga accccaaaag ttgaactatt
    76081 taatggactg tttccgatat tctactggag atagtccatt taactttagt tttatccttt
    76141 tgttattata ccacctaata tactcattta atttactttg aaatatctca attgatcgga
    76201 atttttctcg ataaaaaaac tctgatttaa gcacaccaaa aaaattttct attacagaat
    76261 tatctaagca atttcctttt ctagacatgc tttgaataat gttattctct tttaattttt
    76321 tttgatattg tggcatctga tactgccatc cttgatctga atgtagaatc agtgaaccct
    76381 cagttccctt ttttttaatt gcttgttgaa gcatttcttc aatcagttta tatgttggac
    76441 tagttgatat actataacta attatttctc cgttaaataa atcaagtata ggagatagat
    76501 agatttttct tccttttatc ttgaattcag tgacgtctgt cacccatttt ttattgggtg
    76561 tatctactga aaaatttcgt tttaacacat tcttggcaat ttttcctact gttcctttat
    76621 aagatttata tcgctttatt cggatttgac aggtaagtcc catttgggac attaattttc
    76681 taactgtttt atgattgatt gtatatcctt tcatctttaa cgctaaagtg actctacgat
    76741 aaccataaga gtttcttgat tcttttacaa tcgctgtaat ttcttgcttt atcttgctat
    76801 atttatctgg cttatctaat ttttttaccc agtaataata agttgacttc gctaattgtg
    76861 caattgaaag taatagattc aatttaaatt cttttttgag ctgaaggatt gttttaacct
    76921 tgatttcttc ttgctcaaat cttgttcttg aatcaaggtt tct
//
LOCUS       DAFGZE010000004        71385 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.004, whole genome shotgun sequence.
ACCESSION   DAFGZE010000004 DAFGZE010000000
VERSION     DAFGZE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.004"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            190..1254
                     /locus_tag="LTU54_000245"
     CDS             190..1254
                     /locus_tag="LTU54_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL8294986.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            1500..1925
                     /locus_tag="LTU54_000246"
     CDS             1500..1925
                     /locus_tag="LTU54_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL8294987.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            1918..2367
                     /gene="nusB"
                     /locus_tag="LTU54_000247"
     CDS             1918..2367
                     /gene="nusB"
                     /locus_tag="LTU54_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBL8294988.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            2464..3318
                     /locus_tag="LTU54_000248"
     CDS             2464..3318
                     /locus_tag="LTU54_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HBL8294989.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            3319..4656
                     /locus_tag="LTU54_000249"
     CDS             3319..4656
                     /locus_tag="LTU54_000249"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBL8294990.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            4661..4885
                     /locus_tag="LTU54_000250"
     CDS             4661..4885
                     /locus_tag="LTU54_000250"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBL8294991.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            4887..5777
                     /locus_tag="LTU54_000251"
     CDS             4887..5777
                     /locus_tag="LTU54_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL8294992.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            5786..6604
                     /locus_tag="LTU54_000252"
     CDS             5786..6604
                     /locus_tag="LTU54_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBL8294993.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            6711..7160
                     /locus_tag="LTU54_000253"
     CDS             6711..7160
                     /locus_tag="LTU54_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL8294994.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            7178..8860
                     /gene="recN"
                     /locus_tag="LTU54_000254"
     CDS             7178..8860
                     /gene="recN"
                     /locus_tag="LTU54_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBL8294995.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(8915..9031)
                     /locus_tag="LTU54_000255"
     CDS             complement(8915..9031)
                     /locus_tag="LTU54_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HBL8294996.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(9104..10060)
                     /locus_tag="LTU54_000256"
     CDS             complement(9104..10060)
                     /locus_tag="LTU54_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL8294997.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            10231..10599
                     /locus_tag="LTU54_000257"
     CDS             10231..10599
                     /locus_tag="LTU54_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HBL8294998.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            10769..11200
                     /gene="mraZ"
                     /locus_tag="LTU54_000258"
     CDS             10769..11200
                     /gene="mraZ"
                     /locus_tag="LTU54_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBL8294999.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            11221..12180
                     /gene="rsmH"
                     /locus_tag="LTU54_000259"
     CDS             11221..12180
                     /gene="rsmH"
                     /locus_tag="LTU54_000259"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBL8295000.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            12185..12586
                     /gene="ftsL"
                     /locus_tag="LTU54_000260"
     CDS             12185..12586
                     /gene="ftsL"
                     /locus_tag="LTU54_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBL8295001.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            12587..14779
                     /locus_tag="LTU54_000261"
     CDS             12587..14779
                     /locus_tag="LTU54_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL8295002.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            14805..15767
                     /locus_tag="LTU54_000262"
     CDS             14805..15767
                     /locus_tag="LTU54_000262"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBL8295003.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            15781..17142
                     /locus_tag="LTU54_000263"
     CDS             15781..17142
                     /locus_tag="LTU54_000263"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBL8295004.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            17168..18256
                     /gene="murG"
                     /locus_tag="LTU54_000264"
     CDS             17168..18256
                     /gene="murG"
                     /locus_tag="LTU54_000264"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBL8295005.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            18271..19491
                     /locus_tag="LTU54_000265"
     CDS             18271..19491
                     /locus_tag="LTU54_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HBL8295006.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            19647..20972
                     /gene="ftsA"
                     /locus_tag="LTU54_000266"
     CDS             19647..20972
                     /gene="ftsA"
                     /locus_tag="LTU54_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBL8295007.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            20995..22236
                     /gene="ftsZ"
                     /locus_tag="LTU54_000267"
     CDS             20995..22236
                     /gene="ftsZ"
                     /locus_tag="LTU54_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBL8295008.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            22253..22930
                     /locus_tag="LTU54_000268"
     CDS             22253..22930
                     /locus_tag="LTU54_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBL8295009.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            22944..23543
                     /locus_tag="LTU54_000269"
     CDS             22944..23543
                     /locus_tag="LTU54_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBL8295010.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            23558..23845
                     /locus_tag="LTU54_000270"
     CDS             23558..23845
                     /locus_tag="LTU54_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBL8295011.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            23894..24676
                     /locus_tag="LTU54_000271"
     CDS             23894..24676
                     /locus_tag="LTU54_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBL8295012.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            24697..25404
                     /locus_tag="LTU54_000272"
     CDS             24697..25404
                     /locus_tag="LTU54_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBL8295013.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            25666..28464
                     /gene="ileS"
                     /locus_tag="LTU54_000273"
     CDS             25666..28464
                     /gene="ileS"
                     /locus_tag="LTU54_000273"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBL8295014.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(28513..30039)
                     /locus_tag="LTU54_000274"
     CDS             complement(28513..30039)
                     /locus_tag="LTU54_000274"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBL8295015.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(30054..30701)
                     /locus_tag="LTU54_000275"
     CDS             complement(30054..30701)
                     /locus_tag="LTU54_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBL8295016.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(30885..31214)
                     /locus_tag="LTU54_000276"
     CDS             complement(30885..31214)
                     /locus_tag="LTU54_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HBL8295017.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            31391..32119
                     /gene="sfsA"
                     /locus_tag="LTU54_000277"
     CDS             31391..32119
                     /gene="sfsA"
                     /locus_tag="LTU54_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBL8295018.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            32135..33148
                     /locus_tag="LTU54_000278"
     CDS             32135..33148
                     /locus_tag="LTU54_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8295019.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            33148..34425
                     /locus_tag="LTU54_000279"
     CDS             33148..34425
                     /locus_tag="LTU54_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL8295020.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            34488..37190
                     /locus_tag="LTU54_000280"
     CDS             34488..37190
                     /locus_tag="LTU54_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8295021.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            37342..37659
                     /locus_tag="LTU54_000281"
     CDS             37342..37659
                     /locus_tag="LTU54_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8295022.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            37689..38009
                     /locus_tag="LTU54_000282"
     CDS             37689..38009
                     /locus_tag="LTU54_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL8295023.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            38117..39577
                     /locus_tag="LTU54_000283"
     CDS             38117..39577
                     /locus_tag="LTU54_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8295024.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            39645..39866
                     /locus_tag="LTU54_000284"
     CDS             39645..39866
                     /locus_tag="LTU54_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295025.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            39897..40079
                     /locus_tag="LTU54_000285"
     CDS             39897..40079
                     /locus_tag="LTU54_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HBL8295026.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            40079..40492
                     /locus_tag="LTU54_000286"
     CDS             40079..40492
                     /locus_tag="LTU54_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBL8295027.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            40615..41796
                     /locus_tag="LTU54_000287"
     CDS             40615..41796
                     /locus_tag="LTU54_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295028.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(41867..42031)
                     /locus_tag="LTU54_000288"
     CDS             complement(41867..42031)
                     /locus_tag="LTU54_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295029.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            42185..42258
                     /locus_tag="LTU54_000289"
     tRNA            42185..42258
                     /locus_tag="LTU54_000289"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42219..42221,aa:Arg,seq:cct)
     gene            complement(42327..43466)
                     /locus_tag="LTU54_000290"
     CDS             complement(42327..43466)
                     /locus_tag="LTU54_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8295030.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(43765..44400)
                     /locus_tag="LTU54_000291"
     CDS             complement(43765..44400)
                     /locus_tag="LTU54_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295031.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(44513..45148)
                     /locus_tag="LTU54_000292"
     CDS             complement(44513..45148)
                     /locus_tag="LTU54_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HBL8295032.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(45182..45643)
                     /locus_tag="LTU54_000293"
     CDS             complement(45182..45643)
                     /locus_tag="LTU54_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HBL8295033.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(45773..46204)
                     /locus_tag="LTU54_000294"
     CDS             complement(45773..46204)
                     /locus_tag="LTU54_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL8295034.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(46222..46542)
                     /locus_tag="LTU54_000295"
     CDS             complement(46222..46542)
                     /locus_tag="LTU54_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295035.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            46841..47617
                     /locus_tag="LTU54_000296"
     CDS             46841..47617
                     /locus_tag="LTU54_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL8295036.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            47632..47835
                     /locus_tag="LTU54_000297"
     CDS             47632..47835
                     /locus_tag="LTU54_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295037.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            47851..48189
                     /locus_tag="LTU54_000298"
     CDS             47851..48189
                     /locus_tag="LTU54_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HBL8295038.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            48176..48355
                     /locus_tag="LTU54_000299"
     CDS             48176..48355
                     /locus_tag="LTU54_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295039.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(48398..48868)
                     /locus_tag="LTU54_000300"
     CDS             complement(48398..48868)
                     /locus_tag="LTU54_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295040.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            48955..49653
                     /locus_tag="LTU54_000301"
     CDS             48955..49653
                     /locus_tag="LTU54_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HBL8295041.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            49831..50172
                     /locus_tag="LTU54_000302"
     CDS             49831..50172
                     /locus_tag="LTU54_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295042.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            50165..50836
                     /locus_tag="LTU54_000303"
     CDS             50165..50836
                     /locus_tag="LTU54_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HBL8295043.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            50842..51528
                     /locus_tag="LTU54_000304"
     CDS             50842..51528
                     /locus_tag="LTU54_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295044.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            51531..52280
                     /locus_tag="LTU54_000305"
     CDS             51531..52280
                     /locus_tag="LTU54_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295045.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            52292..52561
                     /locus_tag="LTU54_000306"
     CDS             52292..52561
                     /locus_tag="LTU54_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295046.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            52567..52731
                     /locus_tag="LTU54_000307"
     CDS             52567..52731
                     /locus_tag="LTU54_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295047.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            52724..53023
                     /locus_tag="LTU54_000308"
     CDS             52724..53023
                     /locus_tag="LTU54_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295048.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            53023..53325
                     /locus_tag="LTU54_000309"
     CDS             53023..53325
                     /locus_tag="LTU54_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL8295049.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            53365..53730
                     /locus_tag="LTU54_000310"
     CDS             53365..53730
                     /locus_tag="LTU54_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295050.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            54220..54360
                     /locus_tag="LTU54_000311"
     CDS             54220..54360
                     /locus_tag="LTU54_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295051.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            54357..54545
                     /locus_tag="LTU54_000312"
     CDS             54357..54545
                     /locus_tag="LTU54_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295052.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKNLI"
     gene            54553..54747
                     /locus_tag="LTU54_000313"
     CDS             54553..54747
                     /locus_tag="LTU54_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295053.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(54780..54992)
                     /locus_tag="LTU54_000314"
     CDS             complement(54780..54992)
                     /locus_tag="LTU54_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295054.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            55117..55320
                     /locus_tag="LTU54_000315"
     CDS             55117..55320
                     /locus_tag="LTU54_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295055.1"
                     /translation="MVKVREQFKIVSDEDNVYKIKIVNINEYREADHKYLVDLYMNDK
                     YLGEGFCGDSLFDDERILKEETE"
     gene            55317..55589
                     /locus_tag="LTU54_000316"
     CDS             55317..55589
                     /locus_tag="LTU54_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HBL8295056.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            55590..55775
                     /locus_tag="LTU54_000317"
     CDS             55590..55775
                     /locus_tag="LTU54_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295057.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            55762..56109
                     /locus_tag="LTU54_000318"
     CDS             55762..56109
                     /locus_tag="LTU54_000318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295058.1"
                     /translation="MNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVLKIE
                     QNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIRGGV
                     CEISLVKRLPRTR"
     gene            56069..56545
                     /locus_tag="LTU54_000319"
     CDS             56069..56545
                     /locus_tag="LTU54_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HBL8295059.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(56691..57419)
                     /locus_tag="LTU54_000320"
     CDS             complement(56691..57419)
                     /locus_tag="LTU54_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBL8295060.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            57467..57745
                     /locus_tag="LTU54_000321"
     CDS             57467..57745
                     /locus_tag="LTU54_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295061.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            57747..58133
                     /locus_tag="LTU54_000322"
     CDS             57747..58133
                     /locus_tag="LTU54_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295062.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            58130..58510
                     /locus_tag="LTU54_000323"
     CDS             58130..58510
                     /locus_tag="LTU54_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL8295063.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            58622..59074
                     /locus_tag="LTU54_000324"
     CDS             58622..59074
                     /locus_tag="LTU54_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBL8295064.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            59071..60798
                     /locus_tag="LTU54_000325"
     CDS             59071..60798
                     /locus_tag="LTU54_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL8295065.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLSRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            60862..62049
                     /locus_tag="LTU54_000326"
     CDS             60862..62049
                     /locus_tag="LTU54_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL8295066.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            62021..62590
                     /locus_tag="LTU54_000327"
     CDS             62021..62590
                     /locus_tag="LTU54_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBL8295067.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            62603..63967
                     /locus_tag="LTU54_000328"
     CDS             62603..63967
                     /locus_tag="LTU54_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL8295068.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            63969..64250
                     /locus_tag="LTU54_000329"
     CDS             63969..64250
                     /locus_tag="LTU54_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL8295069.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETDQ"
     gene            64228..64554
                     /locus_tag="LTU54_000330"
     CDS             64228..64554
                     /locus_tag="LTU54_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL8295070.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            64544..64882
                     /locus_tag="LTU54_000331"
     CDS             64544..64882
                     /locus_tag="LTU54_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295071.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            64872..65237
                     /locus_tag="LTU54_000332"
     CDS             64872..65237
                     /locus_tag="LTU54_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL8295072.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            65244..65870
                     /locus_tag="LTU54_000333"
     CDS             65244..65870
                     /locus_tag="LTU54_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8295073.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLAKVGGAG"
     gene            65870..66334
                     /locus_tag="LTU54_000334"
     CDS             65870..66334
                     /locus_tag="LTU54_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295074.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            66529..68838
                     /locus_tag="LTU54_000335"
     CDS             66529..68838
                     /locus_tag="LTU54_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL8295075.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            68850..69554
                     /locus_tag="LTU54_000336"
     CDS             68850..69554
                     /locus_tag="LTU54_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8295076.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            69551..>71385
                     /locus_tag="LTU54_000337"
     CDS             69551..>71385
                     /locus_tag="LTU54_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8295077.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
BASE COUNT        24305 a        11731 c        14901 g        20448 t
ORIGIN      
        1 tttggaaaac gatcataagt ttttacaaaa tagtaaacta aaaggagttg cagcgagact
       61 gtgactcctt tttggcttta tttatttaaa ataaaaagaa gttttccgga aatttttatt
      121 taactgtcgt gtacgaagaa aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg
      181 agtttcatta tgatgctacg tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg
      241 gattcatttt tggttaccag tccgtacaat cttcgttact taacgaactt tacaggtacg
      301 acaggattag ctgttattac tttggaaaaa gcatttttta ttactgattt tcgttatacc
      361 gagcaggcgg cagcgcaggc tcaagggttt gaaattatta aaaatgtcgg acctattttt
      421 gatgaggtag caaacttagt acgcaaagaa gaactgcact cattagcttt tgaggaaaca
      481 acagtctcat ttttagaata ttcggtttta gaagaaatca tcgatgcgga attagtacct
      541 gtttcacaga tgatcgaaga actcagagaa gtgaaagatg aagaagaaat cgctattatc
      601 gaaaaagcat gtcatattgc ggatatggca tatgatcaca tcctgaaaat gatccaaccg
      661 ggcatgactg agattgaagt cgcaaatcaa ctagactttt atatgcgttc tcttggcgct
      721 tcaggtgttt cttttgatac gatcgttgct agcggattgc gttcggctat gccgcatggt
      781 gtagcaagca aaaaaatcat cgaacaaggt gacttgatca cattggactt tggttgctat
      841 tacgaagggt acgtttccga tatgacacgt acattcgcta ttggtgatcc tggggaaaaa
      901 ttaaaagaaa tttatcaaat agtcttagat gctcaattag cagttattga tgctgcaaaa
      961 ccaggaatga ctggtgtcca attagatgca gttgctagag attatatttc aaaacatggt
     1021 tacggggaag catttggaca ttctacagga catgggatcg gtttagagat ccacgaagga
     1081 ccaaacgttt cttctcgtgc agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa
     1141 ccagggatct acttaccagg aatcggtgga gtacgtattg aggatgactt gttgatcaca
     1201 gaagatggaa atcgtgtgtt gactcgttca ccaaaagaat tgatcatctt atagtttcat
     1261 caatagaaga agtaagcgta aaaatcagtg ttcacagact ttgttataga aaatcactcg
     1321 atttaatgat acactataga agaactttta agctgtaaga gataataaag ttgggtgttt
     1381 tctccaattc atagcagttt gcagctgatc ggcggcagtt ttcttaccac gtgatcacta
     1441 tgccgcatca tagagaattc ggatgtatct cgtgcttagg caaagggagg aataaataaa
     1501 tggcagatga aaaaaatttg attttaaacg caaaccaaga tttaggagag atcgtgatcg
     1561 ctcctgaagt catcgaagta attatcggga ttgcagcttc taaagtagaa ggcgtatacg
     1621 gaatgcgcgg aacttttgcc agcaatgtca ctgaattatt aggacgtgca gctcacggaa
     1681 aaggtgttta cttagcaaac gaggaagatg gcttgaaagt agatctttac tgttatctag
     1741 aatatggtgt ttcagttcct aaagtagcaa tggaaatgca ggaacgggtc agccagcaag
     1801 tccttttcat gacggatatc gatttatctg aagtcaatat ccatgtagta gctgttgttc
     1861 ctgaaaaatt agaacaacca acaatcgaag aactttttga agacgaagag gaagagaatg
     1921 aataaagaat tatcaagaca cgagattcgg gaaatggcat tgcaggcttt atttccatta
     1981 gacttcaatg cagacctaac aaaagaagat gcgattttca acgcaatcga attagaccac
     2041 cgcgacatga tcaacgaaga tgaatcagag tttgttcctg tttatcttga tacgctagtt
     2101 ggcggtgtct gtgcaaaaaa agatgaattg gataaagtga ttgaaaaaca tttaaaaaac
     2161 aattggcaca tcaatcggat ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt
     2221 gaaatgactt atgtgacaga tgtaccagcg gctgttgctt taaatgaagc aatcgagttg
     2281 gcaaaaacat ttagtgatga ccgttcacgt aaattcgtca acggtgtttt atccaatgta
     2341 ttgaaagaat tagaagctgg ggcgtaagtc ctagcttttt tctatgaatc atcataaacc
     2401 tgttataaat cgggaaaatt ctatggtaga atgaaaacac aaaaaatatc tgggagtggt
     2461 tagatggcag aattgattaa cggaaaagaa ttagctgaaa aaatgcaggc agagattgct
     2521 gaaaaaatcc aaaaattgaa agataatggt atccatcccg gattagtcgt tttattggta
     2581 ggggaaaatc cagccagcca aatctatgta agaaataaag aacgtgtagc aaaagaaatc
     2641 ggaatccatt cattggtaga gcgttatcct gagacgattt ctgaagacga actacttgca
     2701 gaaattgaaa aatacaatca agatcctagt tttcatggta tattggtcca attaccattg
     2761 ccaaagcata tagatgaaga aaagatcctg ttagctatcc gccctgaaaa agacgtagac
     2821 ggttttcatc cgatgaattt aggcagactc cttgcgggta aaccggacaa gattccttgt
     2881 actccatacg gcatcatgaa aatgtttgaa gcttattcta tcgatccggc gggtaaacga
     2941 gcagtagtta tcggacgaag caatatcgtc ggtaaaccga tggctcagct actcttgatg
     3001 gcagatgcaa ctgttactgt tgctcattct aaaaccgaaa atctggctga actggcacaa
     3061 gaagcagata ttttagtcgt agcaatagga cgtggacatt ttgtcacaaa agaattcgta
     3121 aaaccaggtg ctgtagtgat agatgtaggg atgaatcgtg atgctaacgg taagctgatc
     3181 ggtgatgtga aatttgatga agtggagcct attgcaggct ggatcactcc tgtgccaaag
     3241 ggtgtagggc caatgactat cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct
     3301 tttggagcag gtgaatagat ggcacaagaa tatttaactg tcactacatt aacgaagtat
     3361 ttgaaaagaa aatttgatgc cgatccttat ttagaacgcg tatatttaac aggcgaaata
     3421 tcgaatttcc gtttacgtgc caatgcccat caatatttca gcttaaaaga tgaccatgca
     3481 aaaatatctg ctattatgtt caaaggagca ttccaaaaat tacgattcca gccaaaagaa
     3541 ggaatgaaag tccttgtcgt tgggcgtatt tcattatatg aagcgggagg ttcttatcaa
     3601 atctatgtag agcacatgga gcctgatgga ataggtgctt tatatcaaga attggaagaa
     3661 cgaaaagaaa agctgatgaa ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt
     3721 ttcccaaagc gaatcgcggt ccttaccagt ccaagcggag cggttatccg cgatatcatc
     3781 acgacggtga aaagaagata tccaattgtc caattggttt tgttcccaac agttgtacaa
     3841 ggaaaccaag cagcggacaa tgttgtcagg aatatccaga gagtagaaga gatgggcaat
     3901 ttcgatacaa tgatcatcgg tcgtgggggt ggttcgattg aggatttatg gccgtttaat
     3961 gaagagcggg ttgctcgggc gatttatcaa gctaatacgc cggtgatttc atctgtagga
     4021 catgaaacag ataccacgat cgctgattta gtagcagatg ttcgggctgc cactccaaca
     4081 gcagctgcag aattagctgt tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg
     4141 caagctagat tgcagcaggc gtttgtacgc cagatccagc gcaaacaaga acggttcgag
     4201 cgtgctcgac gttcctatgt atttcggcag cctgaaagat tgtatgaagg tcagaccatc
     4261 aaattagacc agttgaaaca gcgattatat caagctacac agcaaattta tcatgcgaaa
     4321 gaaaaacaaa cgatcgcatt aagccaccaa ttggaacaag tggctccgct ttatcgagtt
     4381 aaagcagctc gccaagaaac tgcttatcta gaaaaacgct tagaagagaa aatccaacaa
     4441 tatatgaaac aacagcagca gcgttttgct caagctgttc aatctcttga tttgttgagt
     4501 ccattgaaaa tcatgggaag aggctacagc tatacgacgt taaatgatca agtagtaaaa
     4561 agtgtcaccg agattcatca aggggatttg ctgacgatcc attatcaaga tggaatcgtt
     4621 aaaggacaag tacaagagat agacaaagga gaataaaaat atggaaaatc caacttttga
     4681 agagtcactg caagaattag aaaaaattgt catgcagtta gaacaaggag acgttccatt
     4741 agaatcagct ctagattcgt ttaaacgagg aatggaatta agcaaacatt gccaagatac
     4801 attagctaaa gcagaagaga ccttgacgaa aatgatgaca gaatcaaatg aagaagttga
     4861 ttttgacgga aacgaggaaa gctgagatga gattaactga cttttcagct attcatctac
     4921 ctgctgtaga aaaagaaatc ttgtcttttt tagatgagca tacaacggat agaggactct
     4981 atgatgcgat gagttattca gtgaaagcag gcgggaaaag aatacgtccc ttattacttt
     5041 taacggccgt tgcttccttt gatgagccta ttgatgttcc cgtctatcaa gtagcagctg
     5101 ctttagaaat ggtgcatacg tattccttga tccacgatga tttgcctgcg atggacaatg
     5161 atgatcttcg tagaggaaaa cctaccaatc ataaagtatt cggcgaagca ttagctatat
     5221 tggccggaga tggtttgctt acaggagcat tccagctaat cagtatggca cacttaggta
     5281 attctcctaa gcttctgctg cttcagcaat tagctgtttg tgcgggatca caaggtatgg
     5341 ttgctgggca agctgcagat atcgagggcg aatcgaaaaa gctttcttta gaagaattag
     5401 catttattca tgaaagaaag acagggcatt tgattcgtta tgccttgctg gctggtggca
     5461 tattagcgaa gcaacctgaa gaaatcttat tgctacttca acgattagcc gaacatttag
     5521 gtttagcttt tcagataaga gatgatctac tcgatgtgat tggtacaacg aaaacattag
     5581 ggaaaacagc tggtaaagat gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc
     5641 tagaaaagac acgtgaggcg ttggagattg agctgttatc cgccaacaag atcatagaca
     5701 aattagaaga ggacgttttt tcttttgatg gcgggctgat gcgccagatg atcaagcaat
     5761 ttgatgtgga gaaatgaggg aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa
     5821 acaaggatta tttgaaacaa gagaacaagc caaacgttca gtgatggctg gtttgattta
     5881 caacgaaaaa aacgagcggt ttgataaacc gggagaaaaa attccagtga gtagtgaatt
     5941 aagagtcaaa ggtaaaaaac ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc
     6001 actcaaagca tttgatctat ctgttgaagg aaaaacattg ttagatattg gtgcatccac
     6061 tggcggattt actgatgcag ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt
     6121 tggctacaat cagctagctt ggaaaataag acaagatcct agagtcgtcg taatggaaag
     6181 agtgaatttt cgttatgcaa aacccgaaga ctttacctta ggaattccag aagttgctgt
     6241 tatcgatgtt tcttttattt cactaaaact gatgctgcct ccattacatg ctattttaaa
     6301 agaagaggga gaagtgattg ctttaatcaa accccaattc gaagcaggca gagaagctgt
     6361 agggaaaaac ggaattgttc gtgaccctca gacccatcaa aaagttttag aagacatttt
     6421 gtcatttgcg gcacaaggtg gatatgatgt gctggaatta agttattctc cgatcacggg
     6481 gggagaagga aatatcgagt tcttggctca cttgaggaaa gtacccgaaa gcggaacaat
     6541 caattcagct attaatatgg cagaagtggt ttcaaatgca cacgaacagt ttgaccataa
     6601 ataaattgta actgtgaaaa tcaagtgaga agtatgcgga ttcttcttta ttataccaag
     6661 gaaataagct atcattgatt aagaagaata tgctaaggag cgaagacgta atgagaaaaa
     6721 aagatcggca tcgtttgatt actcgattgt tgagtgagca agatatccga aagcaggaag
     6781 aatttgttga aatcctgaaa agcaaaggaa tatctgttac tcaagctacg atttccagag
     6841 atataaagga actgaaactg atcaaggtac cggcttcaga tggcggctac cgttatagca
     6901 taccagctga aacaaacgaa gatgtaaata ctaaattgga aaaactatta aaagacgctt
     6961 tcgtggcagt tgaccaaatg gaaaaatttg ttattttgaa aacattgccg ggaaatgctt
     7021 cggcagcagc aaatttgatt gataaacgtt acaagaaaga attattttcg attatcaatg
     7081 atgacgataa tgttttgatg attacacgaa ctgaagacca ggcagtttta ttgaagaaag
     7141 acttcctgca ttacctttaa gttgaggagc gaatgtgatg ctacaagaaa tcagtattac
     7201 taactttgcg attattccgg aattgcggct ttcttttcac gaaggaatga ccgcgctgac
     7261 cggagagact ggtgcgggta aatccattat tatcgatgct ttaggtctgt tagcaggagg
     7321 aagaggttca agcgattata tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt
     7381 tgaactacca aaacaagaag gattctctga actgatggtt gaattaggga tcgaaacaga
     7441 tgaggacaat ctgattgttc gacgggatat gtccttaact ggaaaaaatg tttgtcgagt
     7501 gaatggtcat attatcacat tagctaatct gagaagaatc ggcagttatt tagtagatat
     7561 ccaaggacaa aacgaacatc aggaactgct ccagcctgaa tcacatcttg ctttattgga
     7621 tcgttttgga gatgtagctt tccaacagaa gaaaaagagt tatcagcaag aatatatctc
     7681 ttatcgtgag ctggaaaaaa gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca
     7741 gagaatagat atgctccatt tccagcaaga agaaatagct gctgctgatt tgcaagtcgg
     7801 agaagaagag aaattaaaag aagaaagaga aaaacttagc aattatcaaa aaattgctga
     7861 tggattagct gcaggttacg gagcactgac gaataatgaa caaaacagtg tagatggtgt
     7921 tggcttggcc gtttcagaac tgcaagggat cgcccatcta gatgtggagt atgaagcaat
     7981 ttatgaaaat atccaaagtg cttactattt gctgcaagat gcaatcggcg atatgagtcg
     8041 tcagattgat ttattggagt tagatgagag tcgtttagaa gaagttacgc aacgtttaga
     8101 attaatccga cagttgaaac gtaaatacgg cgaatccatt gagtcaatat tggcttacta
     8161 tgacgaaatc accgaagagc tagcttcttc tgatttttca gaaggtcaat tggacaaaat
     8221 gaaagaggaa ctagagcaaa aagagcttct acttcagcaa caagccgcag atcttcatga
     8281 agcgcgtaaa aaaatcgcaa aagaattgga aaaatccatt ctgcatgaat tgaaaagtct
     8341 gtatatggaa aacactgagt ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa
     8401 tcatgatgga tttgaccaaa tcgaatttta cattacgacc aatccaggag agccactgaa
     8461 gcctttggtg aaagtcgctt ctggtggcga actgtctcgg atgttgcttg ctttgaaaac
     8521 tattttttct tctgagcaag gtgtgacaag tattattttt gatgaagtgg atacaggtgt
     8581 gagcggaaga gttgcacagg ccatcgcaga taaaatttct caaatatcga aatactctca
     8641 agttttgtgc atcactcatc tgcctcaagt tgctgcagtc gccgactatc aatattatat
     8701 tgtgaaagca gtgattgatg gacgtacgca aacttctgtt tcagagttga aaacaaaaga
     8761 aagagaagaa gaaattgcac gtatgctggc tggaagtgag attacaaaac tcacactcga
     8821 acatgcaaaa gaactattga aactcgcaaa aaggacctga gccacctcgc agtggttcag
     8881 gtccttttca gcagctgata gctatatttt atttttacat gacactcatt acagcagtca
     8941 atacaacaga tccaatcgtt aaaatcagca ttgtccaaat gacaaatttt gttactttag
     9001 caaaagtact tgtttgtttt ttttcacgca ttgttttcaa cctcgtcatt aattagttga
     9061 ttcaagtgta caatcttttt tccatttcca caagagggct tttttataaa aacttccctt
     9121 tttttaaata ataggtagtt acgatacaaa taaataatgt taatagaact agccatataa
     9181 aggcatcatc cctttcagca ataggtaact taatgttcat tccatagata ctgccgacga
     9241 ttgtcggtat agttaaaata atcgttaaag aagttaaaat tttcataata ttattcagat
     9301 tattcgatac gattgctgaa aaagtctgat tcatttgttc tactagtttt aattgaatgc
     9361 gagaagtagt cattgcctgc tctgtttcca ccaatacatc acgtaaatga tttttcaaag
     9421 catgattttc ttggaaaagt ttattttgcg ctagttcttt caatgcatct aaattagcag
     9481 aagtcgcttc tctgaaataa acgaggcttt tttggatatc catgatttga taaagttgtt
     9541 tgttttcagt ggatacttga agtttggctt ccaaagtatc gcattgagat tgaagcgtag
     9601 ctaaataact gttgtaagag agtgtgacct gccaaatgaa ttgaaagaag atattgagat
     9661 taggcgaaat atcacttttt ggaaaggtct ggctaaagac tttctttaaa aacagtggtt
     9721 catgattcgt aattgtgata agcttctttt tgggcgtcaa aataatagag agcgggtatg
     9781 tatccacttg aaaataaccg ctaggacttg ttttagcaaa aggatattgt aacaataata
     9841 acgctggctt tgtcaacttt gattgattga attcttctgt acgggaattt tctgcatcgt
     9901 ccaatatgga agtgatgtaa tctgacggaa tttcaaattg atgtgtcagc tgatgaatct
     9961 cttcttgatt aggacgttct acacaatacc agatcgtttg gtcagtttcc tttccggtat
    10021 tacgcagttt ctcattatct aatgtaaaat atttgatcat gattttcctc ctgttgcctg
    10081 tttcttttat tataacggat agggaattgg aatacatgga atgtgtttgt aagatataga
    10141 atataactgc ataaatttaa ataaaatgaa acagttgtgt aaagtatata cagtatactt
    10201 gaagcaacga taaaaaagga gtttacgttc atgggcacat tcacaccact tatcttgttt
    10261 tggtatattt acccggtaat cgttttgttt ggttgccaat ttcttgtaaa attattttcg
    10321 ttaaatcgtc gttttaaaat aaaagcccct gatttggcga ttcctttttt gtggggcggt
    10381 atgcatgcat tgtctagaaa tacagggaca atttctatct taccgttttt ctttatttct
    10441 gttttgctaa tgggaatttg tattgctgtt ttccaagcat attattatga agaaattatt
    10501 tatccgcgtt atttcaagat gacttggcgt ttggtttttc tgttgacaat cgtcttgtac
    10561 gctgtcttga tcattgtgaa catactttct tacctatgaa aaaaatagaa aagctgtaaa
    10621 aagtctttta aaaggctttt tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa
    10681 gttgttcccg gatttcccag aaaaggtggt agaaagtggg ggattgtggt agactaattt
    10741 tagttagtgg aggaatgggg ggctttcaat gttgatgggt gaattccaac ataatattga
    10801 tgcaaaagga cgtttaatcg tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt
    10861 tctgacacgc ggattggatg gctgtctttt cggctatccg atgagcgaat gggaaaatct
    10921 ggaagctaaa ctgaacgaaa tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt
    10981 cttctattcg gctgccactg aatgcgaact tgataaacaa ggacgaatca atatacccag
    11041 cacgttaaga aattatgcag ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg
    11101 aattgaaatt tgggatgaga cacgatggca agatttttct gcagcagcag aagagaactt
    11161 tgacgaaatt gctgaaagta tgattgattt cggactataa gaataggaag aggctgacta
    11221 atgactgaaa catttcaaca ctatacagtc atgttgaaag aaacggtaga tggattgaac
    11281 atcaaaccgg atggcgttta tgtagactgt acactcggcg gtgccggaca tagtgaatat
    11341 ttattaagcc aactaagttc taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca
    11401 atcgatcacg ccaagctgcg tttagcttct tatgtcgaac aaggtatggt cacatttatc
    11461 aaagcgaact tcagagaatt agaagaacgc ttgagtgagc atgtagataa ggtagacggc
    11521 attttgtatg atctaggggt ttcctctcct cagctagatg aagcagaacg tggatttagt
    11581 tatcatcaag atgcaccgtt ggatatgcgc atggatcagt cagctccttt atccgcttat
    11641 cacgtaatta atgaatattc ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag
    11701 gagaaatttt ccaaacaaat tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc
    11761 caaacaactg gtgagcttgt cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga
    11821 aaaggcgggc atcctgcaaa aagaattttt caagctgtgc ggattgcagt aaatgacgaa
    11881 ctcggtgccg aagaggcctc tttagaacag gcaatccggt tattgaaagt caatggaaga
    11941 atcagcgtaa tcacattcca ttcattggaa gacagaattg tcaaaagtat gtttaaagaa
    12001 tatagtacgg tacaagatct accaccggga cttcctgtca tacctgacga attccaacca
    12061 gaactgaaag tcattactcg taaaccaatc cttccttcag aggaagagct aacagaaaac
    12121 aatcgatcac gcagtgcaaa gttacgtatt gcagaaaaaa taagaatgaa agaggagtag
    12181 aatgatggca gaattaaaga aaatgcaaga ttacgaatat gatttacctg aattggtgga
    12241 tcagccggaa caacaaatgc ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc
    12301 accggcaaaa cgattaaagc agatttctgc tttggaaaaa attgctgtgg caagcgtggc
    12361 gattgctatt gttgctttat gtgttttaac agttatgctg cgcacaaata tcagcggagc
    12421 tgaaaaagaa atcacgtcaa tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag
    12481 tttagaacaa gaaaaaaacg aactttctcg tactgaacgg attaaacaaa tcgcagaaga
    12541 aaaaggattg tctattaatg acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa
    12601 taaaattcgg cggtttatgg aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt
    12661 aggtatcatt ttatttgcta cgagtattgg attgttcttt ttatttgccg tccgtttctc
    12721 gtatatcgtt attggaggtc atgttgctgg tacctcatta gctgaaaaaa caaaacagtt
    12781 gtaccaagga agtgaagtag taaaagcaaa aagaggaacg atttatgatc gtaacggtgt
    12841 ggcgctcgca gaagatgcct cctcttattc aattaaagcg atactctcaa aaacatatac
    12901 ctctggggat aaaaaactgt atgttgaaga aaagaatttt gataaaatag cagaaatcct
    12961 gcataagaat ctttctatag acaaaaaaga tgcactgaat attttagagg acggcgcaaa
    13021 aaaagagctg tatcaagttg aatttggtag ctatgggaaa aatatctccc aagaaaccaa
    13081 gcaaaacatc gaagcggaca tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca
    13141 tcaggctcgg atgtatccaa atggcgtctt ttcttctcat tttattggat acgcagttcc
    13201 agacaaagat gagaatggct tagttggtaa actaggtttg gagtctgctt acaatgatat
    13261 cttgagcggt aaagatggta agattattta tcaaaaagat aattttcaaa atcctctgcc
    13321 aggtacagtg gcagaagaag aaaaagctgt cgacggacaa gatatctaca cgactttaga
    13381 cagcagacta caaagttatt tagaaacact gatggatcaa gtaaatgaag aatatcagcc
    13441 agaagaattg acagcagtcc tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca
    13501 acgacctact ttcaatcctg aaacaatgga aggattgact ggaaaagatg ctatctggcg
    13561 taatttcttg gtacaagata gttatgaacc aggatcaacc atgaaagttt tcactactgc
    13621 agctgctatc gaagaaggag aattcaacga gaatgaaacc ttccaatctg gaaaaatcca
    13681 agtagaagat gctacgatca atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg
    13741 tcaagctctt tcatggtcaa gtaacgtcgg gatggtcata ctggaacagc gattaggcgg
    13801 acgatggtat aactatttac aaaaactggg atttggacaa agtactcatt ctggattaga
    13861 tgatgaagta aacggtgctt tacctacatt aaatattgtc gaccgagcaa tgagtgctta
    13921 tggacaagcg gtaggtgtga cgaatttcca aatgatgaaa gctttcactt ctattgcgaa
    13981 taacggtaca atgattcaac ctcgatatat cagcaaagtg gtggatcccc aaactggaga
    14041 agagcgaaca actcaaacag aagttctagg gcaacctttt tcaaaagaaa caacagaaaa
    14101 agtccgtgaa tatatgagag atgttgtcga aagcgaaaac tacggtagtg cttatggtgt
    14161 atatagtgta cctggataca atgtatcggc aaaaaacgga acagcccaaa tcgcttctga
    14221 tacaggggga tatcaaacag gagatacagc ctacctgtat tcgattgtgg agatggttcc
    14281 ttctgaagat ccagactatg tcttgtatct aacaatgaaa caccctaaga catatgatcg
    14341 aatggcattg gctaaaatcg caaatccatt gatgaaacgg gcaatggatt ttaaagaaac
    14401 tgaagaagat tcagatacag aaacaaaaac agaaaaagta tccgtagccg attatcgtaa
    14461 tcttgaagcg gacgttgcag cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat
    14521 cggtaatggt aaaaaagtac aaaaacaatc tactgccaac ggtgatcagt tgatctctgg
    14581 agaaaaactg atcctatata ctggtggtga taagctgatg cctgacgtaa caggatggtc
    14641 aaaagcagat atcatgaagc tggggaaaat cctcggtatt gaagttagtt ttgatggaga
    14701 tggttattgt gtcaaacaag aacttgctcc atatgaaaaa atcactgaag acaaacttag
    14761 ttttacttta gaagaataag aagagaaata ggagagaaaa gagcatggaa tggacacaag
    14821 cattgattcc cattgtcagc agttgtgcaa tgacaattgc cgcaatgccg ctatttattg
    14881 gttattttca aatgaaaaag caaggtcagg cgattcgtga ggagggacca aagtggcaca
    14941 atagcaaagc cggaactcct acaatgggcg gcttagtgtt tctgatcggc tctattttga
    15001 caggaatttg ggttggagct tggcaaaaac agctaactcc tactcttttc atcttgcttt
    15061 ttgtattagc gttatacgga gtaatcggct ttcttgatga ttttataaaa atattcaaga
    15121 aaagaaacat gggactgaac tcgaagcaga aattgattgg acagattcta gggggaatca
    15181 tcttttatct tgtttaccgt tctgaaggtt atccaggtgt attgaatttc ttcggactag
    15241 atctaccttt aggaatcatt tatggtattt tcgctctctt ctggcttgtt ggattttcaa
    15301 atgcagtaaa tctgacagat ggcatcgatg gattagttgc tggcttagga agtatctctt
    15361 ttgcagctta cggtctaatt gcttggcatc agcatcagta tgatgtatta atcatttgct
    15421 taagtgtttt aggaggttta ctgggtttct tcgtctacaa cagaaaacct gccaagattt
    15481 ttatgggaga tgtcggttca ttagcgttgg gcggactttt agcggcaatt tctataatgc
    15541 taaaccaaga atggacattg cttttgattg gtttgatcta tgttatggaa acagcaagtg
    15601 tgatgcttca agttacttcc tttaagctta caggaaaacg aatttttaaa atgtctccaa
    15661 ttcatcatca ctttgaaatg tgcggatggt cggagtggaa gattgataca gttttttggt
    15721 tgatcagcat tgtgacttcg ttgatcacat tatggatcgt ttggtaggag atgacgggaa
    15781 atgaaaaaaa tatcaacata tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc
    15841 ggagtaagtg cggccaaatt attacatgaa ttgggcgcac ttgtcaccgt taatgatgga
    15901 aaaccttttg acgaaaatcc ggaagcacaa gagctgcttt ctttagggat aaaagtaata
    15961 acaggtagtc atccaatcga attgttggat gaagaatttt cattgatggt caaaaatcca
    16021 gggattccct acagtcatcc tttagtagca aaagcccaag aaatgggtat tccggtaatt
    16081 acggaagttg agctagctta cgaagtagcg gaatgcccaa ttattgggat tacaggaacg
    16141 aacggaaaaa caacgacaac aacgatgaca ggactactac tgaatgcagg agcagatcaa
    16201 ggaattgcac gtttagcggg taatattggg tatccagcaa gtggtgtagc gcaagaagca
    16261 aaatcagaag ataaaattgt gatggaattg tctagcttcc aactcatggg aatcacagat
    16321 ttccgccctc atatagcggt gattaccaat atctatgaag cacatattga ttatcatggt
    16381 acaagaaaag aatatgtcaa agctaaatgg aaccttcaaa aaaacatgac agaaaaagat
    16441 tatctgatcc ttaactggaa tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca
    16501 cgcgtactgc ctttctcgac aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac
    16561 tcaatatact ataaaaaaga aaaaataatg gaaatatctg aacttggcgt accaggtaag
    16621 cataatgtag aaaatgcact agctgctatt tctgtagcaa aattgtatgg tatatcaaat
    16681 gaagctatac gagaaacgtt gcattttttc catggcgtac cacatcgtac gcaatatgta
    16741 ggagaaatcc aaggcagaaa attttataat gattcaaaag caaccaatat tttagcgact
    16801 aaaatggctc taagtggatt tgaaacgtct aaggtcgtgt tattggctgg cggattagac
    16861 agaggaaata catttgatga attaattcct tcattaaaag gaatcaaagc aatggtcgta
    16921 tttggccaaa caaaagaaaa gttgatggat gctggaaaaa aagcaggcat cgagacaata
    16981 gtaacagccg attctgtaga acaagcagtt cctttagcat tagaaaacag tacagatggt
    17041 gatgtagttt tattatcacc agcgaatgcc agctgggatc aatacccaaa ttttgaaact
    17101 agaggaaaca gattcatgga agctgtgaac cgattaaaat agaaaaagta gaagcgagcg
    17161 ataaaaaatg aaaattttgg ttacaggcgg aggaacaggg ggacatattt atccagctct
    17221 tgcctttgtc aattatgtaa agactcaaga accgaatgct gaattcatgt atgtgggagc
    17281 aaaacgaggt ctagaaaaca aaatcgtccc tgatacagga atgcctttcc ataccttgga
    17341 gattcaagga tttaaacgaa aaatttccat gcataacatc aagacggtcc gattgtttct
    17401 taagagtatc cgtcaagcaa aaaaaatcct cagtgatttt caaccagata tcgttatcgg
    17461 tactggagga tatgtttccg gagcggtagt gtacgctgca gccaaaatgg gtattccaac
    17521 agttatccat gaacaaaaca gtgtaccagg tgttaccaat aaatttttaa gccgatatgt
    17581 ggatcggatc gctctttcat ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt
    17641 tcttgtaggt aatcctcgag cacaagaagt agcagatact aaaaaatcag atattttgaa
    17701 aacatttgat ttggatccag aaaagaaaac agtcttgatt tttggcggta gtcaaggagc
    17761 acttaaaatc aatcaagcag tatcagtttt tttaagtgaa tatattcctt cttcttacca
    17821 agttttgtat gcttcaggag atagatatta tgaagagatc ctaaataaaa taaatcctga
    17881 aatacaaaat attagtgtcc agccttatat caaaaatatg gcagaggtca tggcttccag
    17941 tgatctgctg gtggggcgtg ctggtgcaac ttcgatcgca gaattcacag ctctggggtt
    18001 accggcagtg ttggttccca gcccatatgt tacgaatgat catcagacta aaaatgcaat
    18061 gagtcttgtt cacgcaggag cagcaaaaat gattgcagat aatgaactaa caggtgaatc
    18121 actaagtcag acagtcaacg aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg
    18181 agcttctaaa gaacaaggaa tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca
    18241 aataatcaaa aaataaaggc ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg
    18301 tctcatccgt cttctgatga aaacaatttg actccttggc aaaaggctaa ccgaaaatat
    18361 ctggcagaac aagagatgaa aaaccaagag ccaaaaaaag aagacataac attagataca
    18421 cccctttcag attctgaaga agaaaaaacg gttcaagagg aacagcaaga agaaagccag
    18481 tccgagaaaa aaatcgaatt ttttgaagag atcaagcaaa agaaaaacgg tggaccatat
    18541 aacggttctt ttttgaaccg gctgcccaat ttaaaaagtc aacggcacaa agtactattt
    18601 cgtcggttag ctttgatcat cactgtattg ggtataccgc tgattttttt gatctattat
    18661 gtttctcctt atagcaaatt acaagcagtt gcagtatcag gcaataagac agtcaacagc
    18721 caagaaatca tctctgatac aaaattgtca ttaggagaaa atgtttgggg acagtatttt
    18781 catcgatcta cttatatcga tcgccttaaa aaagcacagc caagaatcga aacagcaaat
    18841 atacatttca aagggatgaa tgaattcgat ttggatgtaa cagaatataa agagattgct
    18901 ttgatcgcaa aaaacaatca atactaccca gtgattgaaa atggaacagt attagatgaa
    18961 aaagtagcaa atcctacaaa aaatctccct atcttggaag agttcaaaga ctctgcaaaa
    19021 atcaaagaat taacaaagca atacaatcag ttatcatcag aattacaaaa agcaatttct
    19081 gaaatcaagt acacgccaag agcaagcaat aaaaatctga tccaattgaa tatgaatgac
    19141 ggcaatcaag ttattgtaaa tatcaataat cttgcgaacc aaatgaaata ttattcgcaa
    19201 gtagccaaag atatggatga aaaaggtgtg atcgacatgg aagttggtat cttctcgcat
    19261 ccttacggta gcactcaaga aacggaagaa tcgggccaat cggaagaaac aactgaaagt
    19321 gaagaaaatg cggatcaaac aaacgaatca agtgaagagg cagaaacgca aaaagcacaa
    19381 acacctggat cgtctagcac aaacggatca tctgattcaa atgattcaag tgaaacaagt
    19441 caaatagaag ataatccaag tacaactccg tcaagtttaa atgaaaattg ataaaacaac
    19501 tttaaaaagt aagaatgtac tttctaaaaa ccagtaattt atggtagaat tggaagcagt
    19561 gaatatatct attaataagt atattttaaa acggacgcta gttgttcgct tgtatatgga
    19621 atagtaagta ggagggaatc ccattcatgg caaaaacagg aatgtatgta ggccttgata
    19681 ttggaacgac atctgttaaa gttgtcgtgg ctgaatatat cgacagccaa atgaatatta
    19741 ttggagtagg aaacgcaaaa tcagaaggga ttaaccgagg cattatcgtt gatatcgata
    19801 aaacggtcca agcgatacaa cgagcagtac gacaagcaga agaaaaagca ggtatccaga
    19861 tcaaaggcgt aagtgtcggt ttgccagcaa acatgcttga agtagaaaat tgccagggaa
    19921 tgattgcagt taacggagat tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt
    19981 cagctgccct tgttcgttca atacctcctg aacgtcaaat cgtgtcaata ttgccacaag
    20041 attttactgt cgatgggttc gagggaatca aagatcctcg tggaatgatc ggcgtaagat
    20101 tagaaatgta cggtctgttg tttacaggac ctaaaacgat catccataac atacgtaaat
    20161 gcgtggaaaa tgccggatta atcgtcaacg aaatggtgat tactccttta gcgctgactg
    20221 aatcaattct ttctgacgga gaaaaagatt ttggtacgat cgtcattgat atgggtggcg
    20281 gccaaacaac aacggctgtt atgcatgata agcaattgaa gtttaccaat cttgaccaag
    20341 aaggtggcga atttgtcact aaagatatct ctattgtatt aaatacgtca tttaacaatg
    20401 cagaagcgtt gaaaataaac tacggggatg catacccaga acggacatca ccagatgaag
    20461 aatttccagt agatgtgatt ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt
    20521 cagaaattat ttcagctcga atggaacaga ttttcaacaa agcaaaagat gcattggacc
    20581 aaatcgaagc attagagttg ccaggtggcg tcgtattgac aggcggagct gctagtcttc
    20641 ctggtgttgt ggacttggca caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa
    20701 atcacatggg gctgagaaac ccggtattta caaatgtaat aagtatcgtg gattattcag
    20761 caaacttgag tgaagtgtat caattggcaa aaattgctgt tacgggtgaa acctcagcag
    20821 ctcgtcaaat ggtagttgaa caagaaacaa caaacacata tgagagctat gaagcacctg
    20881 aagaaacaat ctacgatgag ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa
    20941 tcaagggttt ctttacaaat atttttgatt aattcatcct gggaggaata agatatggaa
    21001 ttctcaattg ataataatat caatgacggc gcagtaatca aagtcatcgg agtcggtggt
    21061 ggcggtggaa atgctgtaaa ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt
    21121 acagccaaca cagacgtgca agcattaaaa aattcaaaag ctgaaacagt tatccaatta
    21181 ggccccaaat atactcgtgg tttaggtgct ggttctcagc cagaagttgg acaaaaagcg
    21241 gcagaagaaa gtgaacaatc cttacgcgaa gctttagacg gtgcagatat gattttcatc
    21301 actgcaggaa tgggtggagg aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg
    21361 aaagaattgg gcgcattgac tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg
    21421 aaacgtggcc gttttgctgc agaaggtatc gctcgtttga aagaaaatgt ggatacatta
    21481 ttaattatct caaataaccg tttattagaa gttgtagata aaaagacacc gatgcttgaa
    21541 gctttccgtg aagcagataa tgttttacga caaggtgtac aaggtatctc tgatttgatc
    21601 acagcgccag gttacgttaa ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa
    21661 ggtacagcat tgatggggat cggtgtggct agtggagaag atcgcgtagt agaagcaacg
    21721 aaaaaagcaa tctcctctcc attgcttgaa acatctattg atggagctga acaagtactt
    21781 cttaacatca caggcggttt ggatatgaca ttgttcgaag cacaagatgc ttcagatatc
    21841 gtagcaaatg cagcaactgg cgatgtaaat atcattctag gtacatctat caatgaagaa
    21901 atgggcgatg aaatccgtgt gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa
    21961 cgcaagtcta gccgaccagc tagacaagca caaatgcaat cacctgctca aaagactgta
    22021 ttagatatgg atcaagctaa accaacttct tcagaagaag aaaacagttt tggcgattgg
    22081 gatatcagac gtgaacaaaa tgttcgtcct agagttgatg attccaattt tgacaatatc
    22141 gaaaaaaaag aatttgatac attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa
    22201 ttaaatacgc caccgttttt ccgtcgtaaa aaataaagga gaggaagagc acatgattgc
    22261 tgacaacttg cagaaaatca agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga
    22321 tccgaaagaa gttacattag tagctgtaac aaaatctgtt gaatccgctc aagctgaaga
    22381 attggttaga gctggcgtta tgaattgtgc agaaaaccga gtagataaat tattagaaaa
    22441 gaaacaagat ttatttgatt ttcctgaaat aaagtggcat ttaattggta atttacagcg
    22501 cagaaaggta aaattgattg taaatgaaat tgattacttt catgcgttgg acagtttaag
    22561 attagcagaa gaaatccaga agcggttaga gaaagaatta tcctgtttta ttgaagtaaa
    22621 tgtttctggt gaggaatcaa aacatggaat ttcaccagat gagcttttac cgtttgtgga
    22681 atctttagct gcctgcacaa aaatcaaggt tgttgggttg atgactatgg cgcctaaaga
    22741 agcttctgat gagcaaatcc ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt
    22801 tcaagcaaaa ggttatgccc atgcaccatg tagtgaatta agtatgggga tgagtcaaga
    22861 ctttcaaatt gcgattgaag aaggtgcaac atttatccgt gtaggaacag cgctattcaa
    22921 agacgaataa aggaggaaca aaaatgccgc ttttaaacag aggagcaatt gctaattttt
    22981 tcggattagc tgatgaggaa gagtacgaat accaagatta tcctgaaaca acaaaaaagg
    23041 ctgtacagcc aactaaaaaa aatacaacac agaatcaatc aactcattct gtatctgctg
    23101 cgaatactca tgaaaaacaa aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc
    23161 agccggctga aaagaaaaaa gtaattgaac tacatcaatc acctacaaaa actgtagcga
    23221 aagaaagaac agcttcaaga acctctacac aacctaaagc aaatcttgct ggaaaaatca
    23281 ctgtaatgga accaagagct tattctgaag ctatggcgat tgctaaacga atcattgctg
    23341 gagaagcaac cttagtgaat tttcatttag tagaagaaaa acaagcgaga agaatcgttg
    23401 attttctaac aggaacagtt tatgcactag atggagatat caaacgtgtt ggagatgaga
    23461 tttttctttg tacaccgact gggatggaga ttgacagttc aaccgctcaa tcttttgcag
    23521 atgcgaattt gtttgatctg taagaggagg aactacgatt tatactattg cactattact
    23581 tcatttactt caacgggcag cctatttcta tagcattatc ttgatggtat atgcattgct
    23641 ctcttggttt ccaggcgggt atcaatcagg gctaggacga ttattagcta agatctgcga
    23701 accgtatatc agtttgttcg atcgtctgcc tttgaggatt ggacccttgg atttcacaat
    23761 tttaggggca atcctgatct tgaacttagc cgtacaaggt ttagcacaga tcgttatatg
    23821 gataactgtc catttagttt attgattgaa gcaacaaaat ctttattctc agaatagata
    23881 ggaggggaaa attttggatg caaatgtata ccagcacttt cgggcagatg aacgtacatt
    23941 tatcgattcg gtaggcgatt ggattgaaca ggttcaagga caatatgcac cctatctgac
    24001 cgaatttctt gacccaagac aatcctatat tttagaaaca ctgattcgac aaagcagtga
    24061 tttgcgtttc atgttttatg gcggatatga acaggcagaa agaaaaagat gtttgatttt
    24121 ccccgattat tacgaacctg tagaggaaga ttttgaaaca gcattgtatg aaatccatta
    24181 tcctagcaaa tttgccagcc taagtcatgg gaaaatcctt ggaactttag tgggaacggg
    24241 gatcaaacgc tcttactttg gcgatatcat ctcggacggt gatcgctggc aagtatttat
    24301 tgcaaatgag gtggatcatt ttgttgctgc acaagtaaca aagattggaa aggttgctgt
    24361 acgcttggaa ccaatcaaat acacagaaat gattatgccg aaagatagct ggacacaaga
    24421 aagaggaaca gttagttcca tgcgactaga cagtgtgatc tctgagattt ataatatttc
    24481 cagacagcgg tccaagcagc tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa
    24541 tacaagacct gattttgtac ttgaactatt agatattatt tcagtcagag gttatggcag
    24601 agtccagatc caagaacttg aggggaagac gaaaaaagaa aagtacaggg tactgttcgg
    24661 tgtattacgt aaataaaata aaaaggtggt ttactcatgg ctttaactcc attagatatc
    24721 caaaataaga cattcccgac aaaaatgcgg ggatataatc aagacgaagt agatgatttc
    24781 ttagatttag ttgttcgtga ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa
    24841 gcagtcaaac attctgaaga aaaacttgag tacttcaatg aattaaaaga tgctttgaac
    24901 caatcaatca tcgtagctca agatacggct gataaagtaa aaacaagtgc aagcaaggaa
    24961 tccgaagtga tcgttacttc tgcacaaaac aaagcagatg agttagttgc gaacgctgaa
    25021 aaacgtgcac atcaattaac aactgatgca gaagaaaaag caagaaaaat cttgacagat
    25081 gcaacagaaa aagcaagaca gcttgcgaca gaaactgaag atcttaagaa aaaaacacga
    25141 gtattccatc aacgtatcag cttgatgctt gagtctcaat tagaacaagt gaaaagtccg
    25201 gaatgggatg aaattttaca accattctcc agctacgtga ctgacagcca tgaagtgatc
    25261 aaagaagttt tagcaaaaca gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca
    25321 ggagtcatag aagcacctat gacggcattt gatacacagg ctattgatat ctcgatcgtg
    25381 cctggtgaaa atgaaaacga gtagaacaga aaagcacttt gttattctta aagcgagccg
    25441 atgagagtgg aagttcggta agacactgca tggtgaaaga tcctctactt gagacctgct
    25501 tttccgaaat tagagtaaga agcagcggct gacctcgtta ttggtcacag agagtttgca
    25561 ctagatagat gcaactaaat ttgggtggta ccacggcatg ttcgtccctt gcggctgaac
    25621 atgctttttt gtgtccggaa aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa
    25681 accttgcaat taggaaaaac aaaattccct atgcgtggta atttgccaaa ccgagaaggc
    25741 gagtggcaaa aagaatggga agaaaatcga atctaccaaa aaagacaaga gttgaacgaa
    25801 ggaaaaccta gctttgttct tcatgatgga cctccatatg ccaatggtaa tatccattta
    25861 ggacattcat tgaataaaat cagtaaagac attatcattc gttcaaaatc aatgtctggc
    25921 tatcgtgcac catatgttcc aggatgggat acacatggat tgccgattga acaagtattg
    25981 gctaataaag gcgtgaaacg taaagaaatg tccatggcag aatacttgga aaaatgtcgt
    26041 gaatatgcac tttcccaagt agataagcaa cgagcagatt tcaaacgcct aggagttgca
    26101 ggagattggg aacatcctta tgttacatta gatccttctt atgaagctgc acaagtacgc
    26161 gtatttggta aaatggctga aaaaggctat atttacaaag gattgaaacc aatttactgg
    26221 tctccgtcaa gtgagtcttc tttagcggaa gctgaaattg aatataaaga tgtaaaatct
    26281 ccatctattt ttgtagcttt caatgtcaaa gacggcaaag atattcttga tgaagataca
    26341 gcttttgtca tttggacgac aacaccatgg acattgccag caaaccaagg tattgctgta
    26401 aatccaaaat ttacgtatgt attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa
    26461 gatttgctag aaacagtcca atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa
    26521 tttgctggac aagaaatgga atatatgact gcaagtcatc cattctatga acgtgaatca
    26581 cttgtgattt taggcgatca tgttacttta gacgctggta ctggattagt ccatactgct
    26641 ccaggacatg gggaagatga ctatatcgct ggtagcaaat ataaactatc tgtagtgtca
    26701 cctgtagact caaaaggatt gtttacagaa gaagcacctg gatttgaagg tatcttttat
    26761 gacaaagcaa atcctatgat cactgatcta ttaaaagaaa aaggagcttt gctgaagcta
    26821 gatttcttta ctcatagtta tcctcacgat tggcggacga aaaagcctgt gatctatcgg
    26881 gcaactccac aatggtttgc atctatcgat aaattccgtc agaatatttt ggatgaagta
    26941 gaaaaagtag agtgggttat cccatggggt aaaacacgtt tgtataatat gatccgtgac
    27001 cgcggcgatt gggtaatttc ccgtcagcgt gcatggggag taccacttcc gattttctat
    27061 gctgaaaatg gagaagcaat catcacacca gaaacaatcg aacatgtggc aaaattattt
    27121 gctgaacatg gatcaattat ctggtttgaa aaagaagcga aagaacttct tcctgaagga
    27181 tttacccacc caggttctcc taatggcgaa tttacaaaag aaaaagatat catggatgtc
    27241 tggtttgatt ctggttcttc tcatgaagct gttttacgtc agcgaccaga attatcattc
    27301 ccagcagata tgtatttaga aggatcagac caatatcgtg gctggttcaa ctcaagtatc
    27361 acgactagtg tggctatcaa tggtgtagca ccatacaaat cagttatttc tcaaggattt
    27421 gctttagatg gtgaaggccg taagatgagt aaatctttag gaaacatcat tgcaccagaa
    27481 aaagtgatca aacaatttgg tgcagatatc cttcgcttat gggtatctag tgtagattat
    27541 gaagcagatg ttcgtgtttc tatggatatc ttaagtcaag tggcagaagt atatcgtaaa
    27601 atcagaaata cgatgcgttt cttattagcg aatacagaag atttcgatcc taaaaaagat
    27661 actgtttcgt acaatgattt acgttctgta gacaaatata tgaccgttcg attgaaccaa
    27721 gtaatcaaag aaatccgaga agaagggtac gacaaatata acttcatgca tatttaccgt
    27781 acagttatga acttcttgac ggttgatcta tcgtcattct acctggattt tgctaaagat
    27841 gtagtttata ttgaagcaga agatgattat caacgtcgtt gcatgcaaac tgttttctat
    27901 caaacagctg tagctttgac aaaattattg actcctatca ttccgcatac agcagaagaa
    27961 atctggtcat tcctgaaaga agaagaggag tacgttcagt taagcgagtt tcctggttat
    28021 gaagagtttg caaaccaaga agaattgatg gatacctgga ctgctttcat ggacttccgt
    28081 gataatgtct taaaagcatt ggaagaagca agaaacagca aattgattgg taaatctttg
    28141 gaagcaaaac taactgttta tccaaaacaa caagttcgtg aaatgttaaa agctttggat
    28201 gcagatattg cacaattatt gatcgtttca ccagactact ttgaagttat ggcagcagat
    28261 gaacaagtgc cagataacgc tatggtattt gatgatgttg ctattacagt ggaaaaagca
    28321 gacggggaaa cttgcgatcg ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa
    28381 cttcctcatc tatgcggtcg ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta
    28441 gctgaaggat tcgaagaaaa gtaaaaggga aaagggggtg tgacatgttc tttgtcacac
    28501 ccccttttgt tatcaatcgt ttaattttcc acgttcacga taccagttat ctggttgcca
    28561 gatccattcg aatccgtctt tttcaagtag ttcgaaagct gctttaggtc ccattgatcc
    28621 agctttgtag ttgggaaaat ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac
    28681 gatatgccaa gattgagcga cttcttccca atgagagaag ttcgttccat caccattcaa
    28741 cgcatctaat agtaattttt cgtaagcttc tgggctattt tcaaccattt ctgcactatt
    28801 tctaaattcc aatttgattg gttccgtttc aaatccttgc cctacttctt tcccattcaa
    28861 ggataaagaa aatccttcag ttggctgaat atagatcgtt agaacattag gtggaagtgt
    28921 cgtatcgtca catggttcat cgacagatgt tttgaataca ttaacaggaa cttgtttgaa
    28981 tacgatgttg atccgagtcc ctttttcagt caaacgtttg cctgtacgta catagaaagg
    29041 aacacctgac caacggaagt tatcgatagt gaatttgcct gcgacgaatg tttcagtctc
    29101 tgattgagca tccactttgt cttcttcgcg atagcccttg aattgtacac catccaattc
    29161 accttgagca tattgcccgc gaacgaagtt ttctaacact tcttcttcgt tataaatgcg
    29221 gatagctttc aatgctttga tcttttctgt acggatttct ttttcggaaa atgctactgg
    29281 cggttccatt gcaagtaatg ctacaacctg taaaatatga ttttgcacca tgtctttaag
    29341 tgcgccacta tgatcgtagt agcccccgcg atcttctaca ccgaggtttt cgccaaaagt
    29401 aatttggata ttgtcgatat agcgattgtt ccattgagcc tcaaaaatat tattggcaaa
    29461 acggatagct gagatgtttt ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat
    29521 atcttgttct gggaatactt ctctgatttg ctggttcaat tcaaaagcag actggtaatc
    29581 agaaccgaac ggtttttcta tgatcaaacg attgaagcca ttttttgtca taatattttg
    29641 tgatttcaaa tgagaaacga tcgttccaaa aaattgaggt gccatagcca aataatagac
    29701 acggttaccg tttaattgat agtgtccatc taatcggtca gacaattctt ttaatgtctg
    29761 ataatgcgtt gaatcattta catcatggga ttgatagtaa aaatggcttg caaaggtagt
    29821 tgcttcttca tctgaaggat tcaatgcacg gattgtttct ttgacaactt cacggtagtg
    29881 ctcatcgctc cagggacggc gagcggttcc gattacagca aaatgctcgc ctaaatttcc
    29941 ttttctgtaa aggcggaaca aagacgggta caatttacgc tgggctaggt cacctgtacc
    30001 gccgaatatc gtaaacaata catttttttc attagacatg tggatacact cctttatatt
    30061 ttatcgacaa aaatcgtgct ggccgcctta aagctgacgg ggattttctt tctatcattt
    30121 tggatagtga tcggtccttc ataagctgcg atgtcaacga tctcatattc ttcgttgata
    30181 ttcaggtcaa tagtgactaa gtaatccaac aattcttttt catcaagcac tcgtgcgata
    30241 cgcacttttg taccgacagg ataggactcc aatgtttgtc ggcgttcttc atggacaact
    30301 tcctcttttt taggaatcga tcctccatga ggacaatgtt caggatgatt caaatagttt
    30361 tctaaatgat ctgcaagatg ttccgaagtc acatgttcca atacttcagc atcatcatgg
    30421 acttcattcc agctatagtc taaatgttca actaaaaaaa cttcccataa gcgatgtttg
    30481 cgaatcagac tgctagcttt ttgcagtccg cttgcagtca attgtacacc ttgataaggg
    30541 gaatgttcta caagctcttc tttcacaagt ttagaaatca tttcactgac agatgcagct
    30601 gagacatcta gactagatac gatttgttta ttgttgactt tcgttgtatc tccacctagt
    30661 tctaaaatga ttttcaaata atcttctcga tttggtgtca tattcgatca tcctttcatg
    30721 gtaaaaaacc atcattagta agtgtaacaa aaaaaacgtt aattgactat ttttctaaat
    30781 aaaaaacaaa ggatttttag acgtttttac aacaaaaaac gaaagatgat agagaagact
    30841 cattctttcg tttataaagg atatatatat acattgggag ggatctattt ttcagataac
    30901 aacttatatg aagtgtctgc agattcgtca taaagagttt gttcgggata agaaaagata
    30961 agataaaaac catactgatc acgtacgact aaacgatatt catctatttc taccacagat
    31021 gtgccaagag actgaccgtt gatcaaaaac gtgtaatcag atgtaatctg taattgttgt
    31081 ttttgctggc tatcactaaa gaaccatgta ccttgatact ttgagagtcc ttctgacttt
    31141 tccgcttgtt ttttcttttt caagtaaata aaacttgaga gaacggtaat gagtgaacca
    31201 agaaaataga tcattttttt tatcaccaaa tcactccttt atgagaaaat aatcgcatag
    31261 acatattctt tcttctttat ttcattgtac cgaataaaag ctaaaatttc tctaagactt
    31321 tcgactggtt tttttactgt ttttctacaa attatgatag gatacatagt aggaagaaag
    31381 gtgacataat ttgaagtatc agcaagtgga aatagctttt tttattgacc gcccgaatcg
    31441 gtttatagca cattgtatga aaaaagacgg agaaataatt attgcgcatg tcaaaaatac
    31501 aggtagagga aaagaggtcc tgataccagg cgctgaagtg gctattgtat ttgctcctgg
    31561 acctaaacgg aaaaccgcct atgatctgat tgctgtaaaa aagaagggtg actggtttaa
    31621 tatcgatagt caattgccta atcggctggc aattgatggt attttagacg gcacgatcct
    31681 tttaccagga ttaactggtc catttacggt atacaagcgt gaagtaactt atcttcactc
    31741 caaatttgat atttatttag agacggtggt cggacaaaaa gcatttgttg aagtcaaagg
    31801 tatgacactt gaaaataaag cgattggtgc ttttcctgat gcgcccacac tcagaggatt
    31861 gaaacatata ggagaattaa cgtatgccgc acaagatggt tatgccgctt atgtcttgtt
    31921 tattgctcag ttcgaacatc ttcatctggc aacgatccat gaagaaatgc aaccagcact
    31981 agctgatatg gttcgacatg cgcagcaaag cggagttcaa atcctcgcct ataattgtca
    32041 agtatcggca gatgaggtaa ctgtcaagca ggccattcca tttgatgtag atatcccttt
    32101 tgaagatcct atacaataga aagaaggtaa aataatgatc aacttaggta ttatcggaac
    32161 aaattggatt actcatcaat ttgtccaagc agctttaagc acaaaaaaat atgaattgac
    32221 agcggtatat tctagacatt tggaaaaagc acaggaattt ggttctcatt atgaagggga
    32281 tatcgaattc gcaacagatt tgaagacttt tttcaatatt actcatatgg acaccattta
    32341 tattgcttct ccgaatagtc ttcactttag tcaagctaag caagcaattt tggcaggaaa
    32401 aaatgtgata gttgaaaagc cagctttttc gacgccaaaa gaaatggatg aaatcatcca
    32461 tttggcaaat gaacacaatg tgtatttttt tgaagcagca agaaatatcc atgaatcagg
    32521 ttttaaaaag attgccgatt acttaccatt aaaagacgag ataatcggtg caaatttcag
    32581 ctatatgaaa tattcttcgc gctatgacca agtattagat ggagaagaac cgaatatctt
    32641 ctcgactcat ttctcaggtg gtgcattgat ggatttaggt gtttaccttg tatatgcagc
    32701 tgttgcttgg tttggaatgc caaaagaagt ccattatttc ccaagaaaga tttctactgg
    32761 tgtagatggt cttggatggg ggatcttacg ttatgagaat tttgatgtat cgatccagcc
    32821 aggtaaaatc ggtgattctt atttaccgtc agagatttat tttgactcag gaacgctagt
    32881 gatgaatgga gtcaatgcaa ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat
    32941 cattgatttg gcggtaacag ctgcagaaaa tccaatggca gatgaagcag aggattttgc
    33001 caatgtcatg gctcatccga aagatccagc ttggggattg ttgtatgaag agtggatcga
    33061 gctagctaga aatgtgaacc aagtgattta tgatttaaga aaaaatggcg gcatcaaatt
    33121 cgatgcagat aatgaggaag atttttaatg aacgaatttg tacaagtatt tcccgaaaat
    33181 tggcagaaaa aatatgagga taaagggttt atctcacctt ctcttattca acgagcagtt
    33241 tttcagccac taagtaataa acaaagtata gtagcggttt ctcctacagg ttcaggtaaa
    33301 acactggctt atctttggcc tctactacta aacgtggaac caggtgaagc aagtgcgttg
    33361 gtcatttttg cttcttctca agaactagct attcaagtag cagatgttgc acgtgaatgg
    33421 ggaaaagaca aagagttgaa agtccaatct ttggtgggcg gagccaacgt caaacgtcag
    33481 attgagggtt tgaaggaaaa accagaaatc ttgattggta caccaggaag aatattggaa
    33541 ttgatgaaag caaaaaaaat aaaagcacat caagtgaaaa caatggtatt tgatgaagca
    33601 gaccagctat ttgacgctgg gaacagccaa atcattgatc aaattcttca tcaagctcca
    33661 acagagtatc agctagcttt tttttcagcg acagcagatc gttcccttga aagcattgaa
    33721 aaaatcactg ggaaaacgtt tgagacaatt gacgtgacag cagaggacga ttctcgaaaa
    33781 ggactgagac attacttttt acgtgtaccg atacgaaaga aagatgacta cttgagacgt
    33841 ctcacgcata tagaagattt tcatggtctg attttcttta atcaactgac tgaattaggc
    33901 gtaatggaag aaaaattgct gtatagaggt gtaccggtag gaagtctggc ctctgatcag
    33961 aacaagctac tccgaaaagc agcgctagaa caattcaaaa agaaaatggt cagagcactt
    34021 ttgaccacag atgtggcggc tagaggttta gatattacag gtcttcctta tgtagtgaat
    34081 gcagaagttc cgttaagtga agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg
    34141 ggtaatgaag gaatagtaat tacactcgta caagataata atctaagaga tttaaaacgg
    34201 cttttacgtc catcaaatat aacacttgaa gagatttttt tatacggagg taagctgcaa
    34261 actgagcagc caaccaaaga agatgcagct cctgcagtaa ataaaaagta tgcgaaaact
    34321 ttttctggaa aggagcagaa cccagaatat aacaaagaaa aaataagaaa aaaagttaaa
    34381 aataaatcaa aaaaagagag aaataaagga gcaaggcgca aataagcgcc tttttatgct
    34441 atgattatga aaacgggaac aaaattggtt gccagaggag gggttatgtg aaagaacgtc
    34501 aagaagagat cttgcagcta ttgacagaaa accaacaagg attgacagca agtgatgtag
    34561 ctgagcgctt aactatcgat cgaagcaatg caagccgata tttaagtgaa ctatacaagg
    34621 cccatcatat tgtaaaaacc gctggccgac cagtcgttta ctctttgcca acggaaaagt
    34681 caaaatctga tgaagtgcat gtcgactctt ctacgcaagt gacttttgaa acactcgttg
    34741 gagaaaatga ttctttaaaa gtcagcatac agcaggcaaa agctgcgatt ctttatccac
    34801 cgcgggggtt gcatacgatt atttttggag aaactggtac agggaagtcg atgtttgctg
    34861 aatgtatgta tcattttgca atcgattcag agatgctttc tgcagatgcg ccatttgttt
    34921 ctttcaattg tgcggattat gctcaaaatc cgcaactgct ctttggacac atctttggga
    34981 tcaaaaaagg ggcttacaca ggagcagctc aagatagtcc cggtctaata gccaaagcag
    35041 acggcggtat attgttttta gatgagatcc accgtttacc gccggaaggg caggaaatgc
    35101 tgtttacttt cattgataaa ggtatttacc gtccgcttgg agaaagtgca caagtacatg
    35161 aagcatctgt tcagatcatt ggggcgacga ctgaatcttc agaaagtttc ttatctacgt
    35221 tcaatcgacg gattcctatg gcaatcacct tgccgaatct tgcttcacgt tcactggatg
    35281 agcgttatga aatcatctct ctttttatca aacaagaagc gaatcgtttg aatcaacgta
    35341 tcgatatcga aaaagaagcg attcttgcct tcatgcttta tgatgcggaa ggaaatatcg
    35401 gtcaagtgaa acgagacttg aaactagtct gtgcaaaatc ttttttacat taccgtactc
    35461 atcatgagaa aaagctaatc attcgaaaag aagaactttc ccttcaagta caaaaaggat
    35521 tactgaaaat caaggaaata ccggaacgtc tcgaccgatt catggacagc aaaagtcagt
    35581 atttgacatt tgaaccagga tttgctgatg ttgtctggtc acaagatcca gaacgaaaca
    35641 tgcaggtcta taacgatatt gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga
    35701 acattgatct agagaccctc atttcaaaag atgtggatgc ttatttccag acatatgtca
    35761 aagaattgac taaaagttcg attcccaaag atcttttgcc agatgaaatc tggcggttaa
    35821 ccaatcaatt gtatgatgtg gctgaaaaac ggttagaccg tatttataat gaaaaagctc
    35881 gttttgcttt tgctttgcat ctgcaaagta cattggatcg tgtgagagaa ggtcatatga
    35941 tcgtccaccc agatttgaat aacgtacgga aaaattttaa gagggaattc caggtagcaa
    36001 tcgatctttc aacgatcatt gaagaagaag aacaagttga gattcctttt gatgaaatcg
    36061 gctttattag tatgtttctt tctatcaatc taggagaaag tgagcctgtt aaagaaaaca
    36121 aagtagatgt tctcatattg atgcacggca gacagactgc ttcaagcatg ttggagatgg
    36181 cacaagaact attaggaaca aaagtaggga aagcttttaa tatgccactg gttatggaag
    36241 ttcaaacaat gtatgaagaa gtgttacaat atgtcaaaaa ctatcaagag catttgacga
    36301 atggcctact cttgttaaca gatatgggat cattaaacac cttcagtgat ttgatttatg
    36361 aagagactgg cattcggacg aaagctatca cgatgacaag taccatgatt gtgatagaag
    36421 cgatacggat ggcaagtgtt ggaagaagct tagaagatat ttatcaaaat atccagcttt
    36481 cctttgaatc gatcgtacgt gatcagttca aagcggaaaa gtctgaaact ttacagcgta
    36541 aaaaagccgt aatcgtgaca tgctttactg gtgaaggagt agctgccaaa ttatatcagc
    36601 gtattatgcc agtgattaat caaaataagg tagaactgat ccagatgcag tttttagaac
    36661 gtgaatcatt caaaaaacac atcgatgcct tattggaaga atatgagatc aaagcaattg
    36721 cgggaacagt tgatgttgat tatcaaaata tcccattttt ttcagcctat gacgtttttg
    36781 atgatgaaaa actaaatatt ttaaagcgta tagctgatga tgagatcccg acagctaaaa
    36841 ttgtcgattc acttgctgga tcattaagcg atgtcttttc tattgatgaa ctgatccgtt
    36901 cattgcaaaa gacagttcag cagatccaga ccgatttgtc gattatcgtg gaacctggag
    36961 tagatactgg aatcgttatc catttagcct tcatgttaga cggattgatc aaacaagaaa
    37021 gccatcggca atttgagaaa ctgtctattt tctcaaaaat ttatcgtttg gaaatggata
    37081 tcgttcgggc aaacttgatg gcactagaac gaaaatatca ggtaaaaatt ccagaagatg
    37141 atatcgcatt tcttacacaa atgtttcttg aaaataaaat aaaaaaatga acacactaat
    37201 catactgttt acacactaaa catctgtgtg cgcactataa aaaagagcta aaaagcatga
    37261 aaacgctatt tcagctcttt tttttattgg catgcttctt gcgttatata aaagtgtaaa
    37321 aaaataaaag gagtgttaaa aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg
    37381 atgagtacga gtttattggt cacaaaaatg caaaaagcag cagaagaaaa agggatggaa
    37441 gccgatattt ttgcggtctc tgcctcagat gcagataata atttagaatc taagaatgtc
    37501 gatgtattat tattaggtcc tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt
    37561 gcgcctaaag gtattccatt agatgtcatc aatatgcaag actatggcat gatgaacgga
    37621 gaaaaagttc ttgcacaagc agaaaatcta atgaaataaa tcaagtatgc agttgggggt
    37681 ataacaacgt ggaagatcaa caaaatctag aagccatcat ggggttaatc atgtttggcg
    37741 gtaatgcaaa gagcgatgca atggaagcga ttgcggctgc taaaatcggt gattttgagt
    37801 tagcggatag gaaaatcgct gatgcagaag aatcacttat acaagcgcat catgcacaaa
    37861 cagacatgtt gacacaagaa gcacaaggaa atcatatgca agtgacatta ttgactgttc
    37921 acagtcaaga tcacttaatg acatctattg cgtttactga tttagcaaaa gaaatcatcg
    37981 aactttatcg tcgcgtatac agcgaataaa tacaacttct tttttgaacg gaagaagaaa
    38041 aatttttgaa cggatttttt attttgttat aaggagaact ctctttatat aaaaaactta
    38101 taggaggaag taaaaaatga acggactatc aagttggatg gagaaataca tcctgccggt
    38161 tgccgcaaaa atcggagcgc aaaaacattt agttgctttg cgtgatgctt ttatcggcac
    38221 actgcccgca acaatggcgg gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt
    38281 accgcaacag tttaacagta cctatgctga cagtttagca gcagacaaag gaatctttgc
    38341 cgtattgaat gtcatcatcg gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc
    38401 cggattgatc tttgcctttt cttgggggta taatcttgcc cgagcttatg gagtaaatga
    38461 tctagctggg gggatcgttg gtttagctac cttgattcaa ggtatttcct ttggtccagc
    38521 attatcagca gcattagatg tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc
    38581 tgagattggt gcaacgattg cagatggcac actgtcagtt ggattatggg gttggctaaa
    38641 actagatcat ttgaatggaa acgcttattt tacagttatg atcatgggag ctatttcagt
    38701 cattattttt tgtaaattga tgcttgccaa cattacgatc aaaatgcctg attcagtacc
    38761 accagcagta tctaaagcat ttgcagccat tttaccagcg acgatcgctt tatacgtcat
    38821 tgctattatc aattatatcg taggtcgtgt agctgatggg caactgatca ttgatttggt
    38881 gcaaaaatat attgcagaac cattcctagg attatctcaa gggttgggag cagtattgat
    38941 cgtcacagta tttgttcaag tattctggtt ctttggtatt catggaccaa atgtattggc
    39001 accagtgcta gagggaatct ggggacaagc acaactaatc aatattgata ttttccaaaa
    39061 aggttataac ggaaaaactg gtacaccagc cgtgttagat gcaatcgaag atggaaaagc
    39121 ttatatgtgg gtccgcggat cgttcgatgc ttttgcatgg tttggcggat caggcggaac
    39181 gatcgtctta ctgatagcta tcttgatttt ctctaaacgt gcagattatc taacagtagc
    39241 taaattatcc attgggcctg gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc
    39301 aatcgtattg aatgcaatca tgtttatccc attcttgatt gctccagtag tagcaacagc
    39361 aattggatgg acagctactt atcttggcct agtcgctccg gtatcgcagc aagtcacatg
    39421 ggtagtaccg ccgttcttat tgtcattctt agcaacagga gcggattggc gggcaccgat
    39481 cgtaacatta gtatgtatgg tcgtgacttt cttgatttgg gcaccatttg tcatctctgc
    39541 aaacaaaatg gaccctgccg caggcgaaca acaataagat gaacgtgagg agcgggacgc
    39601 tttaagtttc gttcctcata atgaattttc taggaggaac tgaaatgatt acaattgcca
    39661 gtgcaatgca acaagatgaa atcaaagaat tgttagaagg atacaatgaa gaaggaatga
    39721 cttttacatt taaagaaaaa caaggaatca agctgttctt tgaaacaagt gcagaagatc
    39781 cggaagttgc ggctaaaaaa gcaaaggaac taatcaaagc acagccttgg ggaacagtcc
    39841 tttattttca atcaacagct gtttagaaaa tggtttaaaa agataggatg gttgagatga
    39901 taaaaaacgg tttattttta tgtagtattg gttctttgat cgtgttgtat agtttgaatc
    39961 cacattcgca aaggtatgat ttgtatagta tggtcacagg tctttttcta acaggattag
    40021 gaatctattt cttcatgaag gggaaaaaaa gagaagaagc aaaggagaaa aataactaat
    40081 ggaaatcacg aaaaaattaa atattccagc atcgtttttt tacgataaag ttatggactc
    40141 tgtccttttc gatgtacgta aagcgaccgg caaatcagta actagaaaac agctgaaaaa
    40201 tttggagtac gtcaaacaat tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt
    40261 cattgaaaac cagtcttata agtttcgtac atcgacaacg aagaatgaat tcgtcgttca
    40321 ttatcaaatc aaaccattgg acgattcgac ctgtgaggtt cattacacag aaaaaatgga
    40381 gtcttatggc atgcttcaaa agttgaatga tatgctttta ggaacaatct tgatgtattt
    40441 taaacggaga cgtttcaaaa aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga
    40501 aacatggtac tatttagtta ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa
    40561 gatagatctt tcctgcgaat cctcactagc ttttttgtag gaaggggcag caaaatgaca
    40621 aacaaagtaa gagagctagg tgtatcttcg attgaggaaa tgtttgattt agccacttat
    40681 gcatttaatg gggcagatac acctgaaagg cgagaacgtt ttcgtacact tgcggagaat
    40741 tcttggaatt acggattttt tgatgaggag gatcggctaa caagtcaagt catggcgacc
    40801 ccttttccag tgaattttta cggacaagag tacctaatgg ccggcattgg ctatgttgct
    40861 tcttatccag aagcaagggg acaaggcggg atcaatcaga tcatggaaaa gatccttgaa
    40921 gattgccgtg atcgtaaagt aagcttatct tatctggcgc cattctccta tcccttctat
    40981 cggcgctatg gctatgaaca atcatttgat aaaatcagtt atcaactaaa cagtcgtgat
    41041 gtgccgttta tcaaaaaaaa atctggaaaa ataaagcgta tggattttga agatgcaaaa
    41101 gctgcaatca gaagaattta tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc
    41161 gaagactggt ggtatacgta taaattcaag caaaaaggca attaccagtt tgctttgcat
    41221 tttgatgata atgaggaagc aacgggatat atcgtttatc agcttgcaac gtcttccttt
    41281 attattgcgg agtggggatt tttggatcat gatgcttttt gcagccttgt tcgcttcgta
    41341 agttctcaca atggtgcttt tgaaacattt gaatattcat gcggctacgg cggtaatgat
    41401 caaaattatc tattagaaaa tccatttgct tctgtaatga tcacacctta tatgatggcg
    41461 aggatcgtag atatttcttt gtttttggaa aattatccat ttaaaaagag acaagctgca
    41521 tttgccctac aaatagaaga agatcattat gcgaaatgga atgaaggaat cgtggaagta
    41581 acaatcgaaa atggaaaaaa tcatctctca aaagtagaac aaacaaaact cccaatcgtt
    41641 cgaggaagta tccaacatat cacgcagcta ttattagggt atcgtaaagt ggaagagttg
    41701 gtattccaag aaagaataca ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg
    41761 ccacagcaac agccaattct taatgactat ttttgataaa aaacgggtga tgacaatatt
    41821 tttgtcacca cccttttgtt tttcatttgg catttgaagg aaaatgttaa agtaaatcct
    41881 cttttttcgt aaatttgaat tttttgtttt cttttgtttt agggttttct tttttgtggt
    41941 cttggattgc ttctttcgta atcttttccc gatttttttt agatgaatct aagcgttctg
    42001 cgtatacttt ttcttttggc ttatcattca ttggaaagga cctccctaca ttatttcatt
    42061 ctacctctag tttatcatct gttaaggact agtggaaacg ttttgcctaa cttataagct
    42121 gattttttta gcagataagc ttgcaagtaa aaaacaaaca agctacaatt gatttattga
    42181 aattgttcct gtagctcagt aggatagagc aatcgcctcc taagcgatag gtcgctggtt
    42241 caattccagt caggaacgta taaataaccc ctgtatcctt tgcggtacag gggtttgttt
    42301 tgtatttgtg ccaaaaatgt gccaaactag ttatcatttt tatataaact atcgaacaca
    42361 gtattgattt tttctttatc ctgatcttca agctctcgaa caatatgaag ataagtagac
    42421 attgtagttt ctaaactgct atgtcctaaa cgtttcgata cgctaagtat atttactcct
    42481 tgataaagta aaatagatgc atgtgtatgc cttagtccat gcaaagtaaa ttgcttttca
    42541 atccctaatt cttttaattt tttgcgcaaa tatttgctga cggcgtttga tgaaactaat
    42601 ccatttttta agttgaaaaa aacaaaatta tgagggtttt tgatttcaaa attctcgaat
    42661 aattcttttt gattgatttt aaactttttt aagagatcaa tcgtatgttt atcaatggaa
    42721 attttcctat ttgaagtttc gttttttgtt tttccccatt catttaattt ataattccat
    42781 gttttattaa tagtgatgat ttgatcttcg aaatcgatat cttcccatgt taatcctaga
    42841 agttcagcaa atcgcattcc agtggcacca gcaactaaca cgagcatagg agaagagtac
    42901 tgcgcagaaa ggttttcttc tacgagtttc attaaagatt taaattgatc ataatctaaa
    42961 tatttatctt cttttggctt caatgaatct tttcctttga ttactgcttt tctagttgga
    43021 tcaaacggaa ttaatccttc ttctacggca tctttcaacg acgctctaat atggttatta
    43081 aacttaataa cggtagattt tacatggtct ttagcatact tatttaaaaa gcgttgatat
    43141 cccactctat ctaaatcaga aatcaatacc gctggcatat atttctttat gttcattaac
    43201 gtatcttcgt attttctata agtgatagga gatactgttc cttctttata aagttgcatc
    43261 caatcctcaa agtaatctga taataataga tttttgcgat cccctttaag gcctttgttc
    43321 aagttatatt ctaattcgtt ggcggcatct tttgcttctc cttttgtctt aaatcctgat
    43381 tttcttatct tcgtgtattt tccgtcatct tttttgtatg aaatttcgta ttgccaacta
    43441 tttccacgtt tgactaatct tgccataatt gaatcagctc tctttctctg atacaatagg
    43501 aactataaag aagcccattg tttgggtttt attatttttt gcacatcctt tttgcttgcc
    43561 ggcgcaagga tgtgctattt gtcgttttaa tggtataatt ggatgtgtat tagatcatcc
    43621 aatcagaggt gtttattatg attaaagtga tttttaaaag aataactgaa cttccagagt
    43681 ttagattatt tgttgattat tggtatgtct ggttttttat tgttgcattg ctgataggtc
    43741 tagatgtcat attagaaaga aaacttaagt aagagttgag ttaataaatt gaattcctaa
    43801 atgagatatt ttgtagccca tattttgttt gccgacatca cctaatacta tggagctaat
    43861 cgttttttct aacagaccaa aatcagataa tctatttaag ctaccatcta ttagctcttg
    43921 tgaatagcca gacatgctga tatttccagt gaaattttcg tttccacgac tcattaagca
    43981 tgctaacaat tgaatctcaa tcgatgttaa ttgattcagt acaagaagat aatactcctc
    44041 gttatcccat gaagcgctgt taattcgaag taaacaattc ttataaagat ttttatacat
    44101 atctatttta ttttgtgcct ttgatttttc tacctcgtca tttatccctt cgaatattcc
    44161 aatgagttgg tccttgttct ctaagttgaa aacatcttct ggaatttgat cgcgaatgtt
    44221 ttctaatgat tcgtttaaat ttttgtagaa tttctcgatg cgtttgaaac gcttctcgtt
    44281 tttagaccca aaataaagtg actgcacaac gccaccaata taaggaattg ctccaatacc
    44341 gccttcaact attaattcaa gatattcttt tttggttagt ttttcattgc tattatccat
    44401 agttacctcc tattttgtca tttcctttac aagcattgtg actcctgatg gcactctttt
    44461 gtataggttt tattattgtg cacaatctaa ctcttagtgg ggcgtggttc atttattttt
    44521 tacttacttt actattaatc atatcccatt gagcatagga tttttctatg ttcgatcctc
    44581 ttgaactaat ttctcctgta aaagttactt tatcgccctt tgtaaaggta tcagcattgt
    44641 ttaagtcctt cgcaaaaatt acatatgaaa ctttaggtgt tgcagaaata tcattccaac
    44701 ttttatcatc gtactctttg tctgctataa tggctattct agtagtcatt gattcgataa
    44761 cagttccttc aaaagtatat gttttaccac taatatattt agtaaaagct gtattttgct
    44821 cagtagctgg agtaatagaa taataactat ctaagaaagc accccagtca tgattatttg
    44881 tctctaaaaa ttcttcaaat tgagttaggc ttttattgct gctgttagat gtagtagttt
    44941 gagtatcaga tgatgagctt tcctgtttgt tattgtcatt tgcctcgttt gatgaggaaa
    45001 gcgagaaacc tacggtagta aaaataatcc cgataataag gagaatcata ggtgttttct
    45061 tatttgtttt tttgataaat cttacaatta acatgattaa acctataata aataaaacaa
    45121 taccagtaaa accaaatata ccgtccatta atagatcctc tttctctttt gatattgaat
    45181 attataaacc aagcaattct tttttcttaa aatcgaattc ttcttgatta ataatccctt
    45241 catctaacaa ctctttatac tttttaattt catctgcact tgaaatttgt gtttctttgg
    45301 aattattatc taatatagat tgttgcttct ctatatattt ttttaaattt tccatttcat
    45361 tccaatattt ttttgtaaac ataacggtgt tttcatcttt cgtggccgct aatatacctc
    45421 cgcgactttc gtttccgcct agcaaggtaa attgtatata accattactc atacctggtt
    45481 tttttaattg tacggctgat atatttttaa aggggatagt tttttctcct ttaatacctt
    45541 gattaactaa atttataaat ccttttctaa taattgaaat agaatcatca gttactttaa
    45601 tcaaggtttt tcctggagat tttattaaaa tctctttttc cattttttct tcctcatttc
    45661 tgtgatatga ttttttttgt agaagctcat atatgaggaa agagcccgtg ttgcagcacg
    45721 ggctttttta atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat
    45781 attgccttgc gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt
    45841 atctgagctt attctgaata gaataatact ccataaattt ttcgagatta aactgagatt
    45901 catcagtcag ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg
    45961 cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaaaattcaa
    46021 aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct gcagtttcac
    46081 cgtatagatt ttcattcaaa aaaatgattc tgggttttgg atagtagaaa ccaggctctt
    46141 tcatctccat ataaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat
    46201 acatacaaac catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc
    46261 tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgtcttc
    46321 tgataaatcc acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga
    46381 gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat tatttttcca
    46441 ctttgctaga taaccgttag aatatccaaa attaatttct aactgccgta tagatatctt
    46501 tttcctcttc gccaagtctt ttattatttc ataggtattc atcgataaaa caacctttct
    46561 aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata atttgactaa ttctgagtaa
    46621 tgagctatac tatatcttgt aaacagattt aacaactaaa aagacaacaa aaaacactat
    46681 tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca atgtttttat gtcttattta
    46741 attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag aaaaaagttg
    46801 ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat atgaacacac cgcaaatttt
    46861 taatttcgaa caaaacgaag ttcggacaat tttagtaaat gatgaaccat attttgtagg
    46921 caaagacgtt gcgagtgttt tgggttattc aaacactaaa gatgctttgt cgcggcatgt
    46981 agatttggaa gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa gagagatgac
    47041 aatcatcaac gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc cctctgccaa
    47101 aaaatttaaa cgttgggtaa caagtgaagt gttgccagca attagaaaac atggaggtta
    47161 tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca ttcaattagc
    47221 aactcaacta aaagaagaaa gaactggaag attaatcgca gaacaaaaga ttgcagagta
    47281 cgaacctaaa atctcctatt tagatagcat attatcttct acagattcag taacaattag
    47341 tcagattgca gcagattatg ggatgtctcc acaacagatg aataaattac ttcataaact
    47401 aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca tgaaccaagg
    47461 atacacaaaa tctcatacaa ctgagatccc gaaagccgat ggtggcacta aaattgtaat
    47521 gaataccaaa tggacacaga aagggcgtct atttatctac gaattactaa aaaaagaagg
    47581 atattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag tatgactgac
    47641 attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga aagttcaaag
    47701 ttcttaactt acaccatgct tgctgaaaga tttggaatta gcaaaagcta cttatcttta
    47761 attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc gattatcact
    47821 atgtacgaat tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa aatcccaatt
    47881 ccagaaggct atgtaattat ctcccaagtg gattatgagg agttaaaaaa agctgatgat
    47941 actggtagat ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa atatgattgg
    48001 tttacttcta gagttttaaa gaacccaaga tatagaaata ttatcgatat cgagaaaaac
    48061 aaaaatggat ttgtctatta tccagttgaa ggaagagaca catatctatt tttaagaagt
    48121 aaaacacttg aatttttaga aacaaatttt tcggaaatct taaggaggca agcggatggg
    48181 aaaatttaat agagcgctag tgttcagcgc accgctaatc atctacgctt taggactttg
    48241 gggaagcagg caagcgttga taggaacgat tgtttacatg gtttggattt ttatggggct
    48301 tgatgaagct gaagctgagt acagagcgaa aaagccaacc gagggggctg actaaaaatg
    48361 aatagaaaag agaaactaga atgtatatta ctattactca gtttaattct ttcactaatt
    48421 tctctattgg gaagtttgta tttttgatat caacaatttt gtggatttgg gttctattta
    48481 gagtaaatat catttttgtt tggtgattaa ggagtttatt aaaattaatt ttccctactt
    48541 caaatgcaag gtaacaagat atagcacttt taggaggaat atttagcggt acagcttcag
    48601 agtatattct attatcatcg actgatcctc ctttagcaat taatttctta aatggagtag
    48661 ctgaccatct gcggtttaag ttagcggatg aaagttctaa attaactaac atagctggtt
    48721 ctgttgaata gttagaaatg attacttttg tataaatcat atctgcagcg aagtaagaag
    48781 cattaagttc aacttctagt tgagggcgca ttccttttat ttttatccct gttaaaaacg
    48841 tactcaatat aaaacttatt attgacatcc attgaaaaat tgttaagtga aaaaaattca
    48901 aaataaacac caccagtttt taactaaatt ataccaaaaa ggagagaaga aataatgcaa
    48961 gaattagtaa ttttgaaaaa taaagaagct gtgactacga gcttacaagt ggcagaaagc
    49021 ttcgagaaaa aacatcaaca tgttttaaga gatattgatg cactaaaaaa agatgtgtcc
    49081 aattttggac agatgtttgt agaaggtaat gaaccagatt catatggcag aaatcgacga
    49141 gttttcttca ttagtagaga tggatttttc ttgctggcta tggggtttac aggaaagaaa
    49201 gctatctact tcaaacaaaa atacattgaa gcattcaacg aaatggaaga tgttattcgc
    49261 aagaatactg ttcctcaaac aattgaagat atgatgatct atcaattaga agaaatgaaa
    49321 gatgttaaaa aagatgtttc catgcttaaa gatactatgc gaattagcgg acaacaagag
    49381 tttgaaatta agcaaaaagg aaatatgaaa gttatgggag ttctaggggg aaaagaaagc
    49441 cgagcttatg aagaaatcag caaaaaagta ttctcaaaat tttggtctga atttaaacgt
    49501 accttttcaa tcccaagata tggcgagtta cctcgtaaga gattcgatga tgctgtttca
    49561 tttattgaaa tgtggttgcc agaaactgcg atccgtatgg aaattgatca actgaacaga
    49621 caacagagac tttttggtga tgacaatgaa tagagctgaa gcgctaagaa tagggacggc
    49681 aattgctaat tgctggtgga aatactataa accaatcatc ctaagccaac aacatattga
    49741 caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa agagtcacaa
    49801 agaaaacaca tcataaggag attttagcat atggaaaaag aactttccac tctagatcaa
    49861 tatttgattg atcctgattg gggcaagccg aaaattgagg aaacaagtgg tcgaaaaatc
    49921 agacgaaatc ttttgacgaa tgaagaacta gcttgggatc aagatgattt aggcaaccat
    49981 gtaactattt gggatcatgt ttatcttatc catctatcga agcattcgaa taaacctgaa
    50041 tatatttacg tcatcgaaga tggcttgatt gatgcgctag aagagtacga cagagataac
    50101 ttgattgata tctcttatta cggaccaggt aagaaataca ttgctgaaat ggaggcagaa
    50161 tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc gtaagttaaa
    50221 taaaattctc aagaaaaaag gaaattttga ttatttatct tgggctcacg cgtgggagat
    50281 tatgaaaaag aatgatccac aggcaacggt aactattaat gagtacaaac actacagggt
    50341 tgtttctgga actcatcaag actttcttgt tgaggaatat aaaccttttc ttatggatga
    50401 aactgggact tatgtatctg tctcagtaac ggttaaagga cacacggaaa ccgaattatt
    50461 tcctgtttta gattatcgaa accaaccagt tgttaaacca aatgcaatgc aaatcaataa
    50521 ctcattgaag cgatgctttg tgaaagcatt ggctctacac ggactgggat tatatgtatt
    50581 tcaaggggaa gatattccaa caccacctag aatcgataca aagaaattaa acatgctaga
    50641 gacgattcta gaagctttca atgagcagat gggtaaagat atgaccaaaa ccttaatcga
    50701 atatgttaat gagcagacag ataaattagg gctcttagct gataacgttg aaactattga
    50761 acagttaagc tatgagcaat gtgccttgat ggagcgagca atagcagcta agagaaaaga
    50821 attagataag aagtgatatg agtgtttaaa ccattaatcg attcatattc agcggttctg
    50881 aaaaagttca aaggaaaaga cataagcgca accatcaatg aggaagtgaa cattgatcga
    50941 ctaaagacga tgtatgacgg ctacgatggt gatcgagtca ttgaaattcg ttttattgat
    51001 cctagacgtt tcaccgtaca gcaacgaaac ttcatctatg cgctgatagg cgatattttt
    51061 atcgatacag gcatgccaac ggacttctgg aaggaattct tctactttcg ttttgaaggt
    51121 gtcacagggc gcaaaataag cctgaaagac gaatcgaata caactgtgag tgatgccaat
    51181 gtcttagcaa atatcatctt agatttcatc tttgaacatc atattccttt caaagaaggc
    51241 tatgagattt tacctgcgaa tcaagaatat tacttctaca aatgcatcac aaaaagagtc
    51301 tgctgcatct gtggcaaaac aggagctgac atcgatcact ttgacaaagc gctaggaaga
    51361 cgaaagcgca aagaagttga tcatgcagag tacacatttg cagcactctg cagaatccat
    51421 cacacagaga agcacaaaat aggtgtgatt aatttcaaaa ataaatatca aatcaaaggg
    51481 atcaaattaa accagaaaac aatcaaaaag ttaaatattg gagggtaaaa atgacagaac
    51541 atcgaagtta ttatgcgatt ataccagcca acgtaaggta cgacaaaaga cttaaaccaa
    51601 atactaagtt gttatacgga gagataacgg ccttgtgtaa tgaaagaggc ttttgttggg
    51661 caggcaatga gtactttgca gatttatatg gtgtgaataa agagaccata tcgcgatggg
    51721 taagtgattt gattaagttt ggatacttga atcgggaaat catttacaaa gagggtacca
    51781 atcaaataat caataggtac ctacgaatta atcaataccc tattgacgaa aaacgcaata
    51841 cccctattga cgaaaaagtc aaagataata atacatctat taataataca tttaataata
    51901 caaaagaata tataagagag ttaccgcctt cgaaaaaatc gaaggctaag cccgtccgtc
    51961 ataaatacgg agagtataaa aatgttcttt tgtcagatga gcagatggag aaactcaaaa
    52021 cagaattccc taatgattac caagagcgaa tcgaacgact gtcagagtat tgtgaatcat
    52081 ctggtaagac ttataaaaac tatttggcaa ctattcgaag ttgggcaagg aaagaaaaaa
    52141 gtgaacctaa gaacgcaagc agtggataca agcgcacagg aagacgagag aagcttcctg
    52201 aatgggcaat cgaccaagaa gcctatctca agaaaaaagc gctagaacga gctaatagac
    52261 aatcaaaagc accattctaa gaggtggaaa aatgaagatc gattatctag aactaattaa
    52321 tgaaatagcg aattataaaa agggcgagga attagacgtc ctgagagacg tatatgatca
    52381 actcgaagaa gctggaattg aaggaattaa gaatgatcgt tcgagttgga gtaaactcag
    52441 atactatttc gcactctata tcgatggaac gcaattaaga aatttagcat ataccaaatt
    52501 actattcatt gattgtgtta aaggcttgca aaaacatctt aatgaacttg agcaggtgta
    52561 aacaagatgg acctaaagac atttacagca cagatcgaat taatgcatca agaagcttta
    52621 agacaaagtg tgtcgtacga agacaagtgg ctcaacacat ttcatggcgg acgtgagagc
    52681 gcacttgatc aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc
    52741 aatgcagcga attatcgaat tgacttattc agaagattgg caggaagata aaaaaatagt
    52801 tgcagaagtc caaaagctcg gtaaatcaat gtggactgaa aagcccaaac ggagaacgcc
    52861 gagaaagatt gcaatctggc atggtgatcg aattctagta acgggtaccg ctaaacagtt
    52921 atctgaaatt actggtctga gcaaaaatat tatttgggat agagctagga gcttatggat
    52981 tgattcaaaa ggaagacagt ttaggtatgt ggaggagaaa taatggacga actaatcgcg
    53041 aaagtagagc agtgggcaaa agataaggga ttggatcaag cggatttcag caagcaaatg
    53101 ttaaaaacgg tcgaagaaat cggggaagtt gccgcttctc tagctagaaa agatgaacat
    53161 ggtttaagag acggaattgg cgatgtagta gtaaccttga tcatcttggc aatgcaaaat
    53221 gatatggatt tatacgagtg cttggatttt gcttatgacg aaatcaaagg tcggactgga
    53281 aagatggtca atggtgtttt cgttaagtcc agtgacctga aataattcca ttaatggaag
    53341 aaataactga ataggaggat agacatgatt gatgcaaaac aagtgcaaaa acagaaagat
    53401 ggaatgttga tgtttgaagc atatgttcta ccatttctta atcaatttga agttttggaa
    53461 tgctctgcca gcggagaaga attggagtat gttgtaattc gtgaaaccaa agaaaacgtt
    53521 cagaagctaa acgaatttct ttgcactatt aactgttggg atatgatagc tccaggattt
    53581 ttatgtccag ctatgggaga atttttggaa tattgtcgtc ttgaagatgc aggcactttg
    53641 gatttagctt atctggtata caactaccta aacatcaaca cagatcattt gtggtttggc
    53701 actgccgaaa gaaaatgggt agtccgctaa ccaccaatat caatgaaaaa aggcggtttc
    53761 ctgaaagtgg aattgccgcc attgctgtaa ggttctttac gcgactcaag gtcacagaaa
    53821 tataaatgca aatctctttt gaagtgatcc aaagctctcg gaaaatggaa gttttttgtt
    53881 tcaaagaata aatgagattt gcgtcaagaa cctgagagtt atttccaaaa aagcttccaa
    53941 agaaccgaat gagctaagga catcatgtgt aaacacataa tatagctctc ctttcacggc
    54001 taatcataac atgaaaaata ttcgatgtca gtaggaaaaa aggtgaatta tgaaaagaat
    54061 attaaattat cccggcagta acagattctg caattaaact ccgaagctga tgtatcagta
    54121 tcaactgtag aatattatga gcgtggagaa agtcaagcag tcacaaatgt aaaaatcgat
    54181 aacgtcaacg gtcttatctg tttagatgaa ggcgatagta tgaatgaaat tgctgcagac
    54241 catgaattat cagaaactgc tttaattgat caaatcgaat tagctagaca atactttgtt
    54301 ccatatgcag atacctggtc caagcataag aatgacatca tttttccaga ggaacaatga
    54361 gagcaactga tccagtaatt atccttgagg aagccaaatt tatttggact cacgaagaga
    54421 tagagcaagc acgcttgctc ttttctcaag gagttaagcc gagcaaagta gctgaaataa
    54481 tgggtcaaaa gattcttgat gtcggattgc ttttgctcca tctagcagaa aaaaatttga
    54541 tttgaggtga aaatgatgat tcaacttgca ggcatccaaa cagggaaaat ttatttttct
    54601 ggagaaagca aaagcgaagc caaccaatgg ctattgaaaa cttacacgaa caataagaag
    54661 ctaaggaaaa aatatcctga tttgttgcta aaggatgatc agattatgcc ggaaccgatg
    54721 attttgaccc gtaaggaggg aagttgaaac taaaaaacca gccgtgtggg ctggagttgc
    54781 tatttctttt taataatgag tttttctaaa tttaattcat tctcacgagt ttgcgtccaa
    54841 taatcagaat aatattttaa ttgtttaaga gtaggaatgt cgtcttcggt aaaattagaa
    54901 aataattctg gagaattatc tttttttaat tgttctaatc ctgattcttt aaaacgttgt
    54961 tcaacgagtt ttactaaatt gttgatattc atgataaatc acctttttaa atttaatttc
    55021 gatttgatta atcgatacaa gtattatatc aaataaaaaa gctgatttag ggggtcttac
    55081 aaaaacacta aaattttttc aaaaagagga ggaacggtgg tgaaagtcag agaacaattc
    55141 aagatagtga gcgacgaaga taatgtttac aaaataaaga tcgttaatat taatgaatat
    55201 cgagaagcag atcacaaata tttggttgac ttatacatga acgataaata tttgggagaa
    55261 ggattttgtg gagattcact ttttgatgat gagcgaattt tgaaggagga aacagaatga
    55321 tccacgaact aaaaatacta ccagaatatt ttgaagcagt gacgagcgga cgcaaacaat
    55381 tcgagatccg caagaatgat cgtgacttca aagttggtga tcagttaata ctacgtgaat
    55441 ataaaaaata cattaaacat gaattacctg gactaacgag accaacattt agttacactg
    55501 gtgactcata caaagcagag atcacttata ttactgatta catgcagaag tccggatatg
    55561 tagtgttggg gattaaaggg gtgaattaaa tgaaagtata catcttagct atcacagagg
    55621 gcacatggat gttcccagtc ggttccggaa aaatctataa atcaaaaaca gctgcttata
    55681 aagcatttga aaaatacaaa aaagaaaacg gtggcggcac taacgcaaag attctagtgg
    55741 ctgataactg gcatgaagaa ggtgaacgta attgatttac ctgttcattt tcgtggtact
    55801 aactattttt atgctagtaa tcttattcaa aaaaagatga gtaaagacga acgaaaaatt
    55861 ctggccaact tttatttagt attaaaaatc gaacaaaatg tgttttcgga aaggagaatt
    55921 gtatgggaaa aacaaaatca aagattaaga agaaaaaacg acgtttggaa caaaaggcaa
    55981 tccagaatgg aacggctaag aaaaaataaa aaagctaccc ctttcgagat agcagcgcaa
    56041 tactattcta ccataagggg tggcgtttgt gagatttcat tggttaaaag attaccaaga
    56101 actagatgag cagattcttt acttaaagtg gaatcttaat aaaagtaagc ttgaattgaa
    56161 tcgatgggtc tgtggtgatt tagcaaacgt ccgcatcgaa aagaattcga gaacttcttc
    56221 tttagaagag aatatccaaa agatagaaaa tgaattggaa ttgttgatcg agcaaaaaga
    56281 agaaatgtta ttgctgatag atagcttttc tggtatcgat aatcaaattg ttaagatgaa
    56341 atatgttgat caaatgagct tagaagatat tgctgaatct gttgggtata gctcatcgta
    56401 tgtcagacag cgacatgcag aaattagaaa gacattgaac tttttagatg agtatgaaca
    56461 cagacaagct gaccgattga aaaaagaaaa cgaaattgat ttttacaata gcgagaagta
    56521 caaagaacaa ctctctttat tctaaaatta cactgttcac taaatgttcg gtcattgtag
    56581 ctatgtaaac attgttagca gcatgatatt ctattagtgt caaaaaaata tgaaagagcc
    56641 aagatatccc aaccgtttta ttaattggta tctgtggctc ttttattctt ttaattagta
    56701 gttggcataa agttatttcc atttataaat tgatcagcta aatcagcatc ataatcagcc
    56761 gcgataaatt gcgctgctga tttaataaat gcatccatat catgaacatc tttattagta
    56821 tgtctacgaa catagtgcgt ttcatcatta ccaatccaag tagcagcttt ggctaatctt
    56881 tggagtttag gaaaatcatt caaataattt tcaattgttt tacctaataa catttttttt
    56941 attttatctt catcctctga atttttagat attgcataat ctttaattag aaattctaga
    57001 gcttttctat aaccaactcc agcaatttgt tccaattgct cagatttagc ttgtgtggct
    57061 tgagtataaa tatcaacaaa tgtaggagaa actttttcaa tattttctgg taaatctatt
    57121 ttaatcggcg gacggtaata atctttaatt aaaatatttt cagaatatcc ttcatcttca
    57181 ttaaacgtat ttataaattg taaactataa aatttgttgc actcatcatt tatacactgt
    57241 actaatacgc caaagattct atcattatcg aacgcactat atgatgaaac cccaaaatac
    57301 acatacggag tcattatatt tccgcaatgc ggacaactat ttggcaaata aattgtctct
    57361 gtattttgac gatcacgata cttaatatta acttcttgaa gcattaaaac acctcgcatc
    57421 ttttttataa atcataccac aaaatatctg ataggagagt aaacaaatga atgattttca
    57481 tgaagcggta cttagtattg aagtagaatc aagtttggct aaagtttata agaaagcaat
    57541 tgaagcagag aatagtccgt acagagaaaa ttggaatggt aatcatgcac atgtgcaagt
    57601 ggagaatgat gattacagaa ctggaatgaa tacgttagtt atttctttat tgtcacacac
    57661 tctacccaat ctgcaggaaa cgattgaatg gtatgagcga atgggcgcaa aagtaatccg
    57721 tacaaattac aaaggagaga attaaaatgg caatgatcaa aattagaaca tccattagtg
    57781 gaacagagta ttgggattca gaaaagaaaa ggacagtagt tgttccgaaa gatcaagaac
    57841 caaattttga agtaaaagaa gaaaaaggaa ttttgagtga tggcaaatca tttgttgctg
    57901 tcaatggcga actcatagct catagcagtg aaattcttga tagcgatgga aacactgctg
    57961 ctaattttga tggagacgaa gctactaatg atcagtccac tgaagatacg gatgaactgg
    58021 acaatatgac tgcaaaagaa ttgcgtgcat atgctaagaa acatggtatt gatattcctg
    58081 gtgctatccg tgcaaaaggc gatatcataa atcttattcg tgaagtagaa tgaaatactg
    58141 tcagtttgac ggatgcacga acaaaatagc aaagggtatc tactgtactg aacacaagag
    58201 atcaagcaga tcacgcaaga agaagcagca agcaaagtct gtttatcatc atgagaacaa
    58261 accattctat cgaacgcaag catggaaaga tatgcgtcaa tttatttatg aaagagaagg
    58321 tggtcattgt cagcggtgtg gtcagttcat atttggaaag agagcacacg tccatcacat
    58381 tgtaccaatc aaagacaacg aactgcttaa gcttgatcca aacaatctca tgctattatg
    58441 ttcaaaatgt catccaattg ttgaaaacga aacggaagac aaaaaagttt ttccttcgta
    58501 tttcaattga agccccccta ttcattttca aaattttttc gcgtggggag atagggtagc
    58561 ggggagtcac gcgcatcgtt aggtcaaatt tttcaaaaaa caaagggggg tgtatacaaa
    58621 aatgacgact aaagcgcaac gtaaagcgat tattgatgaa aaagtaagtg ctgaaaaagc
    58681 tcgtatctta gaaataatga atttgtctga tttgtacacc atcactcttg atccattaat
    58741 cgaatcatac ttggatattt ttgaaattta ccaacacaaa tatttattgt ggaaggaaaa
    58801 aggatttccg gagacgcaaa aattcacgaa taaatcagga gctactaatc aatcaaaaca
    58861 tccattggca caacaagtag aaacttgggc agataagaag atgaaagctc tagatttatt
    58921 aggattaaca aacaaagcaa aaactggtag actaattgct ggaggatcga ccgcaagaaa
    58981 agatgaagaa attacacgcc cagaagtaaa gccagtagat gaactagcag cgcacagaaa
    59041 taaatggcgt aagaaggcag gtgctgaaaa atgattgaac ctggtgtaaa ttatgctgat
    59101 ttatttgcaa aagaagtaag aaagaaacct aagaagtatc ctaagaccgt tcgtttagca
    59161 atagatcgct ggtatcagtg gaaaaaaaga aaagatattt ggtttgatgt agatcgcgcg
    59221 aatgaaatga tggattgggt agaatcattt attgttcaca caaaaggaga aatggtggga
    59281 aaaccgtttc ttttagaacc ctgggaaaaa tttatttatt cttggattta tggttgggtt
    59341 aaagaaaatg aaaaaggcca aattgtacgc gttactcgtg aggcttatgt ccagattcca
    59401 aaaaagaatg gtaaaacatt gattgctgtc ggttcattag gatatgcaat gtacggagaa
    59461 ggggcgttat cagttgattg ctatgcttgc gcatctgatt ttgctcaagc gcaatatgct
    59521 gccaaacctt ttgcagttac aatcttaaac aatccaatcc tacttgaagg aactaaaata
    59581 tttagggggc caaagggaac tgtttctagt attacttatg actatattca tgaagatatg
    59641 gcttactcaa ataaatttat tgttcaaacc aagaatattg ataatatcga gggatccaat
    59701 ccatattttg tattgaacga tgaattgcat aaacaggaga aaatggaaca gtatgacaat
    59761 tttaagtctg ctcagatatc tttaccacag ccattgatgt ttaatatctc aactgctggg
    59821 aaaggttcgt cgtctgttgg aatgcgtgta tatcgcgaag caaaagaagt gttgaaacgt
    59881 gatgataatg attcaaactt tgttctaatc tatgagccaa ataaaaatta tgattggacg
    59941 gatagaaaag tctgggaaat gtgcaatcct aactggggaa tatcagtcga tttatctgct
    60001 ttagaatcgg catttaaaac agcgcaacgg tcagctcatt ccaaagctga atttctaacg
    60061 aaacacttgg atgtatttgt gaacggcgcg gataatttct ttgaacagga tcaagtagag
    60121 ccatgtttag ttaccacaca agaacttggt gatttaagtg gcgaaccatg ttatatcggt
    60181 ttagatttat cacgtacacg agatttgacc tgtgtatctt taaacttccc aacatgggat
    60241 gaagatggta aagcggttct taaagtaaag caactttatt ttattccgaa tgaagattta
    60301 gagtttcgag aaaaagaaga taatgttcct tacagtgatt tggctgaaca aggtttcgtt
    60361 gaattttgcg acggaaaaat gattgatcaa gatcagattt tgcagtacat agaggactgc
    60421 atgaatttat atgatattca gcaagtgaat tatgatccag cgatgagtga caaacttgtt
    60481 gagaaattag aaaacttagg attggaatgc gttgaagttg ctcaatatcc taaagtattg
    60541 aatgctcctt ttgacgatgt cgaacggttg ttttatgaga aacgaattca atttgataat
    60601 ccattattcc tttattgcac tttgaacgtt gtagcaatta ctaacatcaa tggacaaaaa
    60661 gcgccaagta aacgtcaatc aaagaaaaag attgatgggt tcgtggcgtt tttgtgtggt
    60721 cataaggaaa cgatgaatca aatgacagat attgattcag atgagttaga tgattaccta
    60781 agttccatct atagataaat agaaaggcgg tgaaaaaaat tgaaattacg tgatcggtta
    60841 tcgaatgcag tctattcttt tatggaaaaa cgtggatata tcgaggatat ttttggacac
    60901 tacacacgtt atggtcaaag atatgtgacg gattcatcta tcatggaatc ttctgatgtt
    60961 tatgaattag ttcaagacat atctaatcag gtggcattgg ccacaccaat tgttattggt
    61021 cccgacggca acgaagtcaa agatcatcac ttactaaata ttttgaagag tcccaatgat
    61081 tatttgacag gattcgaatt taccaaattg gaaacaaata ctttattgat caatggtgag
    61141 acattcccat taacggatag ggatcagctt catttggcgt acggtgtaac aactaaaatc
    61201 aatgaacgac ttcaagaaga atttgaaatg aatggtcaaa aaatacctgg tcaaatgatt
    61261 cgacacatta agaacatcgg aactgattca ttaaaaggtg ctggaataat tgatcttgca
    61321 agaaacactc tagaaggcgt tctgagcgct gaaaaagttt tgacggacaa atatactaaa
    61381 ggcggtttac ttgcgttcat gcttaaacta gacgcccaca tcaatccaaa taatagcgcc
    61441 caaacaaaaa ttgtcaaagc tatattggat caactggaag gaacgcaaaa tgagagtgat
    61501 cattctgtta agatgattcc tttgggaaaa ggatattcca tcgagacatt aaaaagtcct
    61561 gttgatgacg cggcaatttt gaattattta ggtgtttaca aaaaagactt aggaaaattt
    61621 ctaggaatca atgttgatac gtatcaatcg ctgatgaaga cagatattga aaaagcgatg
    61681 atgtatctgc acaacaaagc aatcaaacca atattgaaaa acaagagcga acattacacc
    61741 gctctttttt ttatgcctaa ttctggctat agagtggaat ggaaaattaa tattttggat
    61801 tttgttcctt actcaacaaa aacaaatatt ggctacaaca tcgttcgtac cgggattaca
    61861 agtccagata acgtggcaga aatgcttggt tttcctaaac agaatactcc agaaacacaa
    61921 gctatctata tttcaaatga cttatctagg attggccaga aaaatgcaac agatgattcc
    61981 ttaccaacga atgatcaaga cttgaaagga ggtgatggaa atgaagaaga aggaaattcg
    62041 cacgattgac atcaccaatc tttcaacacg ttccgatgaa gaaactcata cgaggaccat
    62101 tagcggctac gctgctgtat tcaacagtca aacactttta tgggatgact taagcgaagt
    62161 gattgttcct ggtgcattct caaaggcaat tagtaattcg gatgttcgtt gtttgttcaa
    62221 tcacgattgg tccaatgtgc taggacgtac taaaagcgga acccttcgcc ttgaagaaga
    62281 tgaacgtggt ttgaaattcg aagttgattt accggacaca acggtagcaa gggacttagt
    62341 taaatctatg gaacgaggag acatcaacca atgtagtttt ggttttgtgc cgactgaaga
    62401 aacgtgggac tacaattctg aaccaatgct tcgaacaatc catgaagtgg aattatacga
    62461 ggtttctatt gtgcctttgc cagcatacga agatacagaa gctgcattaa gaagtcgtga
    62521 tgaattagaa aaaaacgtcg aagaaagaaa aaaattaatc aaaaaaatta atcaagcgct
    62581 agaagcgtag gaggaaaaat acatggataa agaattattg aaaaaaatga aggcacgtcg
    62641 tgagcaacga ttgactgaat tacgtgaaaa agttgaatca ggagaattac gcgaagcaga
    62701 tttagaagcc gtgaaagaag aaattgacag tgttattgat gaattgaacg gaattaaaga
    62761 cgaattaggc gcagattctg gaactgatga aacagacgac aatacagatg atcaatcgaa
    62821 tagtacggat tcagacgaaa gccgttctgg tgaagacaac gatcaagaag aagattcaga
    62881 cagtgaagat agttcagaaa atcgttctgg aatgattact caacagcaac gagatggatt
    62941 acttggatca attaagaacg gattggaggc acgtgcaaaa atgaccaaga aacaaaaaga
    63001 tcaacaacta cgaaaagcat ttgctaattt tgtagctgga aatatttctg aagcagaagc
    63061 tcgagcttta gggattgaag ctggcaacgg ttcagttact gtcccagaag taattgcatc
    63121 tgaagttatt acttatgctc aagaagaaaa tttacttcgt aaatacggaa cagtggtgcg
    63181 aacatcagga gatgtcaaat atccaattct tgtgaagaaa gcagatgcga atgtaaacaa
    63241 gaaagagcgt tcaactgata ttgctgaaac agctattcag tttgatgaaa ttttgcttaa
    63301 tcctgccgaa ttcgatgctt tggcaacagt aactaaaaaa ttattaaaaa tgtctggtgt
    63361 tccagttgaa gatattgttg tggaagaatt gaaaaaagct tatgtgcgta aagaaatcaa
    63421 ttatatgttc aatggtgatg acgctggaaa tgaaaatcct ggtgcattag ccaaaaaggc
    63481 tgtagcattt aaaaaacctt tagatctaac tgcttcaggt gctgggcaaa aattatatga
    63541 tgcattaatc gaatttaaaa atacaccagt gacagaagtg atgaaaaagg gacgctttat
    63601 tattaatcga gctgctttga ctgccattga aaaaatgaaa acagatgatg gatttccttt
    63661 gttgcggcca ttcacacaag cagaaggtgg aattggttac caattagttg gctatcctgt
    63721 ggattggaca gatgcagcag ataaaaaggg tgaaccagac acgccagttt tatattttgg
    63781 cgatttttct gcatttaaaa ttcaagaagt tattggtgcc ttggaaattc aaaaacttgt
    63841 tgaaaaattc tctggtaaaa atcaaattgg attccaaatt tacaacttgc tagatggtca
    63901 attggtttat tctccatttg aaccggcagt atatcgctac gaaattacaa aaccagttgg
    63961 tggttaagat ggaagagcaa actaaagaat tgtctttaga ggaaaaattc aaatcacata
    64021 ttcattttga agagggcatg gatgattctt tgctctcttt ttatttaaat atggcaaaag
    64081 attatgtcaa aacagcaact ggtggccaac aagaatatct tattttgatg gttgccggca
    64141 ttgcctatga atatagagtt tcagaggatg aactcgacaa agctatgaat gccatgacgc
    64201 catttatcgt gcaaggagcg attcaaaatg ccgaagagac agaccaataa tctcagatgg
    64261 aaagccgaat tgctagacat caaaacagga acagacggaa acgatcgtcc aactacaatt
    64321 tacgaattta agcgtctaat attctatgaa gaactcggtg tgacttctca agaaaaatat
    64381 ttatcacagc aagccaagac agacgttgtc agacgaatta aagtcagatg ggataaatcc
    64441 atcacagaga aattaagtgc gctcaaaatt gattctgtaa cgtataacat tactcgcatt
    64501 tatacgaatc ccgatgcaag agaaatggag ttgagtttag cttatgtcga ttagctttga
    64561 tgaattgaaa acagcgctga aatcaacaaa gttagcagtg ttcagagaca aagccagatt
    64621 agggacgatg tatccataca ttgtgtactc aaatgtgagt aacagcaaaa aaatggcatc
    64681 cggtaaagtc tataaaaaat taccttatta ccaagtctct ttttttacac ttggaacaga
    64741 acaggattta gttgttattg aacaagcgct gcaaaatgct ggcattccat attcagattt
    64801 tacaggtata caaggtgatg agaacgacga taccgtaacc aactattaca catatgtgag
    64861 gtgtgtggaa aatgccaagt aataagaatg gtttttctga aatatcggat tatttaggaa
    64921 acctttcaaa agttgatccg aaaaaattat cgttggaatc tttagaagaa gcagctaact
    64981 tttatctaaa gcagttgctt cctaatattc ctaagtcgct cttaaaaaag aaacatatgc
    65041 gcgatcaaat aaaagtagta gttgaaaacg atcaagtgaa agttcaattt gaagagacgg
    65101 ccttttactg gcgctttgcc gaaaatggca caacaaatca acgtgcccaa cattttgcaa
    65161 gtggtacgta tgaacaaaac aaagaaaaaa ttgaagaaat tatgactaga aaaatacttg
    65221 atttatggga agggtgattt taattgggaa aacaagatac tttttatttt gaaggattgg
    65281 acgacatttt aattgcgatg atggcaacgc cagattctgt tggaactgca ccgacttata
    65341 gtgaagtttt acgattgcca attgccacaa aaatcggtgt caaagggaat ggaacagcat
    65401 tagaaaaatg ggcatccagc aaaatgtttc gtcgtgtgtc gcgagaaaca aaacatgaac
    65461 ttgcgttgga tcacgtgggt attccgatag aagtgatgga tgaaatcaaa ggattagttg
    65521 ctaaaagcgg agtaacgttt agcaaaaata ccgcacgaga atttccgtac ttcgcctttg
    65581 gatttattgg aaatattgaa aatggcggta aaaaagcagt ttggtatcct aatacacaac
    65641 tatccaatgt gattgacgaa gagtatgcca ctgcagagga agaaacaaaa attgacgatg
    65701 taactgcaaa tctggtttcc atcggactga aatacaataa tgtgatgtat gcaagttttg
    65761 attcaaatag agacggagct tctctagaat tatttaataa attcattgct caacctgtat
    65821 acgatgaaga acagtggaaa acattggcta aagttggagg tgcgggttaa tggctcggtt
    65881 atctgattat ggaattcacg ttgaagactt aaaaaattct gctgttgtaa ttattcaagg
    65941 cgcagaattc cctatttcat ttactatgca aacgatggaa tttatagcag atgtgtatgg
    66001 tggagattat tcgcaatttg aatctgatat gaacgccatg ctatctaaaa aagaaggaca
    66061 aatttcttct gctaacttat cgcctagtga tctaaaaatc atgcgtgcct tgatttatgc
    66121 catgctgcgt actggtggtt tagaggaaga tccagaaact attttcaaat ttttgggaat
    66181 gagtggagag gtgttgtctg cttatagtac ctgtatggaa atttttgcta gccagacatt
    66241 tcaggtggaa gacctaaaaa aatccaagaa gccacaagac tttcaaaaag cgcaagcaaa
    66301 aagaaagaaa aacaaaaaga atcggaagag ataggaactc cttggagttt ttatatttac
    66361 gttgctctca ctctattgaa ttggagtgag agtttctttt taaagtctac acctaacttg
    66421 tggctcaaat cttatttaca gtggttacaa caaaacaccg attttgaacc acctcaatct
    66481 gtaactatgg ataaatcgcc ttggtggtag aaaggagcgc taagataaat ggctggtaaa
    66541 gaatctgatg tcgttcttaa ttttaaaacg aatggcgaag tcagttattc gaaaacaatc
    66601 aaagaaatca acaaagaaat gaacttagcc gctgccgagt acaaaaacca agtgtctgcg
    66661 atggacaagg atgcaactca aacagaaaaa ttgcgagcgg ctaaacaaaa attagaaaaa
    66721 caattaggat tggctgaaca acgatctcaa atgctgagag aggaatatga aaaatctgtc
    66781 aaagaaaccg gtgaatattc tgctgaatca gaaaaacttt ataaacaatt actcaattct
    66841 gaaaccggag aaaataaact tcgtacagca ttagaacaga cgaacgatgc acttaaagaa
    66901 caaggtgacg tttctgttga tacagcaaaa aaactccaga aaatcgaaga aacaggtgag
    66961 aaagtaaaag gcgttggtga aaaaatgtct gttggagtaa ccgcgcctat tgtagcggca
    67021 ggagcagcag gacttgcagc atttggtgaa gttgacgagg cacttgatac catcattaca
    67081 aaaaccggag caacaggtga tcaagctgat agactttcac agtctttcaa aaacgttggt
    67141 tcaaatactc atttaccttt acaaacggtt ggggaagcta ttggtgaggt aaatacacaa
    67201 tttggattca tggataaaaa actggaagat tcaaccaatt atctcctaca gtacgctgaa
    67261 atcaatgata cagatgtttc gcaatcagca atatctgctc gacaagctat tgatgcttat
    67321 ggactagaat atgatgattt gaattctgtc cttgatgtaa caacgaaaac atcgcagaat
    67381 actggtcaat ctgtagacga cttgatgcaa aaagcaattg atggcgcacc acaaattaaa
    67441 caattagggt tgagctttgg tgaaggggtc actttacttg gacaatttga gcaatctggt
    67501 gttgattcaa gcgcagcttt aagtagttta tctaaagcaa cagtggctta tgcgaaagac
    67561 ggaaaatctt taagtcaagg actagaagaa ctacaagaca aggtaaaaaa tgcaggttct
    67621 gaaacagaag ccatcaatgc agcagcagaa gtattcggga ctaaaggcgg tcctagaatg
    67681 gccgatgcaa ttcgtagagg tactttgaac ctggaagatt tagcaaaaac tgctggagaa
    67741 agtgggggag ctgtaggaga cacattcgat gctactctcg acccaattga tcaagccgat
    67801 caagctatga acaatgcaaa attagcaatg gctgacgtag gtgaagcagt ccaagtaagc
    67861 cttttgcctt tttttgaaaa agcaacttcc ttattacagg aattctctaa atggtggggg
    67921 tctctagatc aagatacaaa gaattggatc ataacaattg ctggtatagc ggcagcagtt
    67981 ggaccagtac taatcgttct tggttcactt atgggatctg ttacaaaaat cgttggggaa
    68041 gttaaaagtt ttataggagt ttggcaagga cttgctggtt tatttggaat gtcgggaggc
    68101 tggtttgcat tagcagttat agctattggc gcattagtta ctggactaat ttgggcatat
    68161 aataacgtta aatggtttca tgatggagtg aattctttct tcaaaggtgt ttcagatgta
    68221 gctgttgaaa tatttaattt tttaagtggt tattttggtg gatttttcga tggtgttata
    68281 gcaaatttca ataattttta taatgctgga aaacgaattt tcagtggttt tctagatttt
    68341 atcactggag tatttacagg gaattggtca aaagcatggc aaggtctagt tgatattttt
    68401 ggaggtattt tcgatggtat tgttgctgta gggaaagctc ctattaatgc catgattgga
    68461 ttgatcaatg gatttattgg tggattgaat aatataaaga taccaaaatg ggttcctggt
    68521 gtaggtggaa aatcattttc tatttcaaaa ctgccttatt tagcacaagg cggtcatcta
    68581 atcaatggac aagcgattgt tggtgaagct ggacctgagt tactgactgc caagaatggt
    68641 aaaaccacag ttactccatt atcagacgaa gaaaaacgca aagggatcag tggaagagtt
    68701 tctggaggta gcattgaaca gcatatacac attggcaaag tcgatgcaaa caatccatct
    68761 gagttagatc gtatgaatcg taaatttgct aaagcaaaca aacaggctat tcgtgatttg
    68821 ggaggtgttc ctatttgagt cgacaattta tgaatccaga tgaaccaaat tttatttgga
    68881 aaaatcggaa tgcagtaatt gatatgaatt gcatcattga ggcagagctt ccagatatta
    68941 tgccgaacaa gcgatacgaa acttacacga ttcagggccg aagcggagaa ttgacggaga
    69001 cgtttgatga ctatgaacct tttgatttgg aaatagaagg gatcactatt ccacattcga
    69061 aactgagaga agtcaaacga tggctcactg gtaaaagcca attaatcaca cataatgatc
    69121 cagacaagta tctagaggct atctgcagca tggataaaga agtccctttc gaaaacgagt
    69181 ggggcttctt ttatacattc gatgttactt ttcgttgtca gccactcaaa agaaaaatcg
    69241 gtgaagcacc taaaattttt cattctagtg ttatggatat ttttgatcat ggtgacgaaa
    69301 ttgcacatcc ctatctggaa atagaatcga acggaggaga tataacgatc gctgtcggtg
    69361 ataaaagcct aacaatactt aatacgttag ctgcaacaat cgtggttgat actcagcttg
    69421 gaaaagcaat acaagaaggt ttgaatttat ttacgcgtgg tgattggcca gtattgcagc
    69481 ctgaatggaa tcaggtaaaa atatccggaa aatttaaaga agtgcggttt tggaatagga
    69541 gtgtgtatct gtgacgcaag aatttattta tgcttataaa aaaatgccag atgatttgag
    69601 cgtgaacgga gcgtcgttag ttgattggga agatttgcca gaaatcaatc gtgtgctgaa
    69661 tggccaatat cgtttttatg gtaactattc aagaagcgga cagtatcgct cttatttaaa
    69721 aaaaggaaat tttataaaag cgaaggtacc agatggatca tggcaatatt ttgaaattta
    69781 taatgtcaaa aagaatttga cctccgtttc tgtaacagct cggcatattg gttttatggc
    69841 caataagaac tttattgtca agtcgtttac ggataacggc aatgggtctc agatcatgac
    69901 caatcttaaa aacagtttgg catttgatca aaaatttaac tatctttcga atgtagggac
    69961 aacgcaccaa tttacagcaa gacaagtcgc gccagttgaa gcaatcattg gttcaaataa
    70021 cggtaatcaa aatttgactg gcgtgactag cgcggaactt gatatggata actatgattt
    70081 gaaactggta aaacagattg gatcagataa tggttttcgg attgattttg gcatcaattt
    70141 agaagcaatc gaagaagaaa ttgatgaaga atcaattgtc aatagtttgt atttggttgg
    70201 cggtgttcct gataacgatt atgatgaaga caaagaacct atcgaatatg gttatttaga
    70261 aattgatgga gtaaccaacg agaatcgacg tattgccaaa cgtgaaaacg gagattgtaa
    70321 aacagttgat gaattgatca aatggggcaa aaccttattt gacaatgatc gtatccatga
    70381 gccaaaagct actcatacag tcagtatggt ggcgttggaa cacacactcg aatacggtga
    70441 catgtatcaa gagctagcgt ctttacactt tggtgatgtc gctcatgtta gggcgaaaga
    70501 gctagatatc gagataaaag agcgtatggt cgaatacact tatttcccaa cgcttgggaa
    70561 atataaggat attgttttag gtaatgattt atcactgtac acttcggcag taaatactca
    70621 agcacaagaa ttgaaaaaga aaatcgacaa tcgaacagaa acgttagttc aaaatgtgtt
    70681 gaatgcgacg gcatggatca ctggtaattc tggtgggcat gtcgtttttc gtccagaaaa
    70741 agctccatca gagattctta tcatggatac tccaaatgtg gcgaatgcaa agcgagtgtg
    70801 gcgttggaat ttaaatggct taggttattc agacaacgga gtaaatggcc cgttcggtat
    70861 tgctatcaca tcaaaaggag aaattgttgc tgacttcatc aaagtaggaa caataaatgc
    70921 agaagtattc gaaacttcct ttaatgctta tggtgatgtg ttaaaacttg taaaaggcac
    70981 gcttcaaatt tggaatgaaa acaaaaaaat catggagcta accaaaaaag gtatggaatt
    71041 ctggaataga aaagaatcca ttggaacgat cggaacaaca gattcagcag gaaatccatt
    71101 tccaaatgct gtaacaccta cacctcttga ggaaaattct ttggtaatac gtacaaatgg
    71161 cgatggaaaa tacattttaa tttctccaac tgcagaaaaa ggatttgtgt tattaggaaa
    71221 cggaaaagca tattatttcg gtgatttaga tattcaggga aaacttactg ttagaggtaa
    71281 agaagtgata ccaggacaaa atggcggtcc atctggcgga ggagaaacac ctggtgggta
    71341 tcctgatgaa ttaaaaacag atgcagaaaa gagagcttgg agaat
//
LOCUS       DAFGZE010000005        63019 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.005, whole genome shotgun sequence.
ACCESSION   DAFGZE010000005 DAFGZE010000000
VERSION     DAFGZE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.005"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..352)
                     /locus_tag="LTU54_000338"
     CDS             complement(<1..352)
                     /locus_tag="LTU54_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBL8295078.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTT"
     gene            complement(565..951)
                     /locus_tag="LTU54_000339"
     CDS             complement(565..951)
                     /locus_tag="LTU54_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HBL8295079.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(1007..1630)
                     /locus_tag="LTU54_000340"
     CDS             complement(1007..1630)
                     /locus_tag="LTU54_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HBL8295080.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(1694..2362)
                     /locus_tag="LTU54_000341"
     CDS             complement(1694..2362)
                     /locus_tag="LTU54_000341"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBL8295081.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(2374..2814)
                     /locus_tag="LTU54_000342"
     CDS             complement(2374..2814)
                     /locus_tag="LTU54_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295082.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(2873..3544)
                     /locus_tag="LTU54_000343"
     CDS             complement(2873..3544)
                     /locus_tag="LTU54_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8295083.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            complement(3729..4127)
                     /locus_tag="LTU54_000344"
     CDS             complement(3729..4127)
                     /locus_tag="LTU54_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBL8295084.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(4124..4897)
                     /locus_tag="LTU54_000345"
     CDS             complement(4124..4897)
                     /locus_tag="LTU54_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL8295085.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(4894..5577)
                     /locus_tag="LTU54_000346"
     CDS             complement(4894..5577)
                     /locus_tag="LTU54_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HBL8295086.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(5744..6631)
                     /locus_tag="LTU54_000347"
     CDS             complement(5744..6631)
                     /locus_tag="LTU54_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8295087.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(6628..7497)
                     /locus_tag="LTU54_000348"
     CDS             complement(6628..7497)
                     /locus_tag="LTU54_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295088.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(7634..8920)
                     /locus_tag="LTU54_000349"
     CDS             complement(7634..8920)
                     /locus_tag="LTU54_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8295089.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            9193..9771
                     /locus_tag="LTU54_000350"
     CDS             9193..9771
                     /locus_tag="LTU54_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL8295090.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            9771..10700
                     /locus_tag="LTU54_000351"
     CDS             9771..10700
                     /locus_tag="LTU54_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295091.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            10697..12433
                     /locus_tag="LTU54_000352"
     CDS             10697..12433
                     /locus_tag="LTU54_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL8295092.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(12496..13332)
                     /gene="pheA"
                     /locus_tag="LTU54_000353"
     CDS             complement(12496..13332)
                     /gene="pheA"
                     /locus_tag="LTU54_000353"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBL8295093.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(13329..13838)
                     /locus_tag="LTU54_000354"
     CDS             complement(13329..13838)
                     /locus_tag="LTU54_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBL8295094.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(13831..15126)
                     /gene="aroA"
                     /locus_tag="LTU54_000355"
     CDS             complement(13831..15126)
                     /gene="aroA"
                     /locus_tag="LTU54_000355"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL8295095.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(15139..16230)
                     /locus_tag="LTU54_000356"
     CDS             complement(15139..16230)
                     /locus_tag="LTU54_000356"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBL8295096.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(16247..17413)
                     /gene="aroC"
                     /locus_tag="LTU54_000357"
     CDS             complement(16247..17413)
                     /gene="aroC"
                     /locus_tag="LTU54_000357"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBL8295097.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(17415..18479)
                     /gene="aroB"
                     /locus_tag="LTU54_000358"
     CDS             complement(17415..18479)
                     /gene="aroB"
                     /locus_tag="LTU54_000358"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBL8295098.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(18512..19534)
                     /gene="aroF"
                     /locus_tag="LTU54_000359"
     CDS             complement(18512..19534)
                     /gene="aroF"
                     /locus_tag="LTU54_000359"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBL8295099.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(20193..21167)
                     /locus_tag="LTU54_000360"
     CDS             complement(20193..21167)
                     /locus_tag="LTU54_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HBL8295100.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(21148..23031)
                     /locus_tag="LTU54_000361"
     CDS             complement(21148..23031)
                     /locus_tag="LTU54_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HBL8295101.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(23049..25274)
                     /locus_tag="LTU54_000362"
     CDS             complement(23049..25274)
                     /locus_tag="LTU54_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL8295102.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVVDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEEKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            25389..26423
                     /locus_tag="LTU54_000363"
     CDS             25389..26423
                     /locus_tag="LTU54_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295103.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(26490..27503)
                     /locus_tag="LTU54_000364"
     CDS             complement(26490..27503)
                     /locus_tag="LTU54_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8295104.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(27581..27769)
                     /locus_tag="LTU54_000365"
     CDS             complement(27581..27769)
                     /locus_tag="LTU54_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBL8295105.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(28209..28337)
                     /locus_tag="LTU54_000366"
     CDS             complement(28209..28337)
                     /locus_tag="LTU54_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL8295106.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(28340..29713)
                     /gene="rlmD"
                     /locus_tag="LTU54_000367"
     CDS             complement(28340..29713)
                     /gene="rlmD"
                     /locus_tag="LTU54_000367"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL8295107.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(29824..30879)
                     /locus_tag="LTU54_000368"
     CDS             complement(29824..30879)
                     /locus_tag="LTU54_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL8295108.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(30889..32319)
                     /gene="gatB"
                     /locus_tag="LTU54_000369"
     CDS             complement(30889..32319)
                     /gene="gatB"
                     /locus_tag="LTU54_000369"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HBL8295109.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(32316..33788)
                     /gene="gatA"
                     /locus_tag="LTU54_000370"
     CDS             complement(32316..33788)
                     /gene="gatA"
                     /locus_tag="LTU54_000370"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HBL8295110.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(33788..34093)
                     /gene="gatC"
                     /locus_tag="LTU54_000371"
     CDS             complement(33788..34093)
                     /gene="gatC"
                     /locus_tag="LTU54_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HBL8295111.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(34199..36235)
                     /gene="ligA"
                     /locus_tag="LTU54_000372"
     CDS             complement(34199..36235)
                     /gene="ligA"
                     /locus_tag="LTU54_000372"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBL8295112.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(36252..38486)
                     /gene="pcrA"
                     /locus_tag="LTU54_000373"
     CDS             complement(36252..38486)
                     /gene="pcrA"
                     /locus_tag="LTU54_000373"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HBL8295113.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            38641..40200
                     /locus_tag="LTU54_000374"
     CDS             38641..40200
                     /locus_tag="LTU54_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBL8295114.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVVGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(40311..42242)
                     /locus_tag="LTU54_000375"
     CDS             complement(40311..42242)
                     /locus_tag="LTU54_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8295115.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(42260..43174)
                     /gene="pfkB"
                     /locus_tag="LTU54_000376"
     CDS             complement(42260..43174)
                     /gene="pfkB"
                     /locus_tag="LTU54_000376"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBL8295116.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(43171..43923)
                     /locus_tag="LTU54_000377"
     CDS             complement(43171..43923)
                     /locus_tag="LTU54_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8295117.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(44082..44291)
                     /locus_tag="LTU54_000378"
     CDS             complement(44082..44291)
                     /locus_tag="LTU54_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295118.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(44302..45630)
                     /locus_tag="LTU54_000379"
     CDS             complement(44302..45630)
                     /locus_tag="LTU54_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295119.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            45766..45921
                     /locus_tag="LTU54_000380"
     CDS             45766..45921
                     /locus_tag="LTU54_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295120.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(45995..46582)
                     /locus_tag="LTU54_000381"
     CDS             complement(45995..46582)
                     /locus_tag="LTU54_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBL8295121.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(46665..47915)
                     /gene="clpX"
                     /locus_tag="LTU54_000382"
     CDS             complement(46665..47915)
                     /gene="clpX"
                     /locus_tag="LTU54_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBL8295122.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(48162..49451)
                     /locus_tag="LTU54_000383"
     CDS             complement(48162..49451)
                     /locus_tag="LTU54_000383"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBL8295123.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(49605..50537)
                     /locus_tag="LTU54_000384"
     CDS             complement(49605..50537)
                     /locus_tag="LTU54_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295124.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHQVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(50694..51353)
                     /locus_tag="LTU54_000385"
     CDS             complement(50694..51353)
                     /locus_tag="LTU54_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL8295125.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(51356..52381)
                     /gene="sppA"
                     /locus_tag="LTU54_000386"
     CDS             complement(51356..52381)
                     /gene="sppA"
                     /locus_tag="LTU54_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL8295126.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(52394..52684)
                     /locus_tag="LTU54_000387"
     CDS             complement(52394..52684)
                     /locus_tag="LTU54_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295127.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            53093..54898
                     /gene="glmS"
                     /locus_tag="LTU54_000388"
     CDS             53093..54898
                     /gene="glmS"
                     /locus_tag="LTU54_000388"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBL8295128.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(54970..56325)
                     /locus_tag="LTU54_000389"
     CDS             complement(54970..56325)
                     /locus_tag="LTU54_000389"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBL8295129.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(56349..57518)
                     /locus_tag="LTU54_000390"
     CDS             complement(56349..57518)
                     /locus_tag="LTU54_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295130.1"
                     /translation="MISKEKRTSILYSFIALLFSITLYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(57515..58402)
                     /locus_tag="LTU54_000391"
     CDS             complement(57515..58402)
                     /locus_tag="LTU54_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBL8295131.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(58591..62283)
                     /gene="nifJ"
                     /locus_tag="LTU54_000392"
     CDS             complement(58591..62283)
                     /gene="nifJ"
                     /locus_tag="LTU54_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBL8295132.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            62482..63003
                     /locus_tag="LTU54_000393"
     CDS             62482..63003
                     /locus_tag="LTU54_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL8295133.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        17464 a        13652 c        11252 g        20651 t
ORIGIN      
        1 ctgttgttcc aagtaaagaa gtcaatacag tcgcactaat tttccctagc ttttcagaaa
       61 ctttcatttt tgtgaatgca ccaatgatcg aaataaacac taaaggcata actagcattt
      121 gcaagaatga tacatatcct tgacctacaa tcccgatcca ttccattgat tgagtaacga
      181 ctttgtcgtc tacaccaaag atcaattgaa gcagaccgcc aaagacgata ccaactccta
      241 atgctgtaaa gacgcgtgtt gaaaattttg tatggcgctt ttgcatacga tagaacacat
      301 ataataatgc cgcaaaaact agaaccacta aaactgtgat gagtgttgtc atttttctcc
      361 tcctcatatt attaatgaca agtccatcga aggaagtaaa ggttcccgag aatgattctt
      421 caaccatcct cctgaaaaaa tttttttcag tgacttttat attttagacg atttttatcg
      481 aaatgacatt ctttttgctt acaattagta ataaaaaaac atacctgtga tagctttaat
      541 ctatcgcagg tatgttttta ctgtctattc ttgtttttct gattgaaaat tcaattttac
      601 cccaggctga tctaaggcga tctctgtcac ctcataagac attcggcgta cgatatcgaa
      661 taacggtgct actagctcgt ccacttcttc ttgtgtgaca tcattttgac cttcaatttt
      721 ccgattgagg atatgattga tctgactcac acttccagat aaaacatatg tatcaaacac
      781 taaacggaac tctaagcgtg ctcctacgat gctatgttct ttcggatagt tttcgatcgt
      841 ttcttttaaa ggcgagaagc ctactttcaa atctgtttct acttcttggt tcggcaatct
      901 catatcataa tgaaatgctt ctacaacttc tttttgtctt ctgatttcca tataatttta
      961 tcactccttt atgaaaatta taacatatct cctttgaaag gggatactac tttttaatga
     1021 tgctctgcaa ggtaaggatt tcattcagat ttcctaccac ataatctgcc gggatctcac
     1081 ccagaaaatg atcgatgtcg taaagacaag tagcgactcc tgcatttttc cctgcttcga
     1141 tatctaaacg acgatcgccg atcatcatcg tgtctgttct ttctaaatga aaagtatcga
     1201 tcaaataatt taaagatgct ggatcaggtt tacgaggaaa gtcctgatcg atccctacta
     1261 cttcctcaat cagatgtgcc aatcgatgtt cttttaacag gccccatgtt gattctgtca
     1321 aacggtgggt caggatgaaa tgacgaccgc cgttgtcttt tagcatttcc agtacttgtt
     1381 tcgtttctgg aaatggacgg gaaacttccg tactttcttt ttcatatcgg tgaaataatt
     1441 cattaaactc tctggcattc aagtttgatt cattgatcaa ataactagta gaatactttt
     1501 tcattttgaa gtagatttct tttttgtcca tactgatacc aaaatctttt agtgcttgcc
     1561 atgcaccatc taccattgcg ggatacgtat caaacaatgt accatcaaag tcccatatat
     1621 aatttttcat gttccttttg ttccattcct ttgcttcttt tttctatctc tcacttttag
     1681 ataaaggtgt tctttaaagt atttttttgt ccagaattgt caaatcatct gctaatgtat
     1741 actggattgg ttgtgcattt gggatatcga tcgtatccat ttgatcttca ggaacatctt
     1801 ctaaatattt tgtcagtgct cggatgctat taccatgacc cacaactagt aaatcttgcc
     1861 gatcttttag ttttggtgct agatgatctt gccataaggg aatgatccga ttcactgtca
     1921 tttccagact ttctccgtat gggatcaaat gcggatccaa gtgatcatat ctgcggtcaa
     1981 atctattttc tgcggttaac ggcggaactt ccgtatatcc tctgcgccat gtttttactt
     2041 gatcttttcc gtatatttct ttcatcttat ctttgttttt acccacaaga tccccataat
     2101 gccgttcatt tagacgccat gtttttatta taggaagcca taactgaccc atttcttcta
     2161 gtgcgacttg tccggtaaga atcgcacgtt tcaaaacaga ggtataaacg gcatcgaatt
     2221 gaagtccggc ttcaagcatt ttttgaccgg caactgctgc ttgttctttt cctttttctg
     2281 ttaaggcgac atctagccat cctgtccagt aattttcaaa atttgcttcg ctttcgccat
     2341 gacgtaataa tactaatttc attcttttct cccttaaaca ttcgtttcga attttatacc
     2401 acatgttttg catgtcacac gtattcgtcc ttttccttta ggtgcacgct gcttttgatg
     2461 acaattcggg cactcaaaaa aagtataact ttttcgatcc attttgactt gctttttgtt
     2521 ttttatctga tgatattttt ctttgactct atcaagatat ttcaaataac gttgattctc
     2581 atttagtctg gggtagattt tttttgatag gaaacgaaat atcatccatc cgaaaaagac
     2641 gatgaacaac aattgtccga ttctataggg caaccaacga cttagcaaac taaagacaag
     2701 cgcagcgatc aatagaccgc gattcaactg atcaaatttc gcatatcttc cctgcataag
     2761 tttgtgatag ttctgctgaa atcgtattag cctttctaac catgtttgca tcatattatc
     2821 tcctccatca tctaatattt attatgacaa aaaaatccaa aaagaaaaag ccttaattgg
     2881 ccttttccat tgcaacttgg tgaaggacat tccgatttct ggtaaagtca atcacaatca
     2941 ctttcttttt atcttctctg ttgactactc gaaatgtgtc cgtcaatgga agaaacagct
     3001 tcttagatgg tagctgttgc atacgtataa aacgatggat catcgtaatc aattgaggaa
     3061 attgaataga cgtgtgaagc agacctggat attttttcaa cagctgtgtc gttttgatca
     3121 gattccgttt tttactgatc acttttaaaa agctttttag aatcttttgc tgacgtctaa
     3181 aaatagaaaa agaattttcg tcatctattt gataacgcat ttgtttttgc agttctgctg
     3241 cagtcagatg gatcttccca tcgtttgtat cgatcattaa accttctgga aacagttcaa
     3301 ccactgctcg atcccaagat tcaagagaaa gctcgatata atcttttact ggtaattcca
     3361 attctacctt aaaaaagtgc aaaacagccg acagcccttc tgcttgatag atttctccta
     3421 atgttagccc ctctagatgg aactctggtg aaaaatgcag caatgtttta ttttcctgca
     3481 attgaacata tagaaatgta gatcgctcaa tcagagtatc ttttaaaaca actaaaaaat
     3541 tcatttttct cccccttttt tcatcatttt taaaatagca tattttgaaa agaaagtctg
     3601 atgcttagag tagaatttca taaaaattta ctatttcata agtattcacg agaaaaatac
     3661 aaacaaaaaa gcggaaataa cgctcatccg ccttcttgtt tgtgtttatt tcctcttcaa
     3721 acgcttgtct atgattcaga cttttctcta tcgttatttt catctaaaag ttcttgcatt
     3781 ccgataattt ctcgatattg atgaatctta gtgataccaa tgactttgtc tacatcaata
     3841 ctaaaacaaa taccattacc tggaatatcg atgttgatgc cttgaccaat cgcatccatt
     3901 actttattcg tcaaactatc gggtactaat gtcaacacaa tatctttttc tggcgaaatg
     3961 tccaaaccta aaaatttccc tgaatcatgt acaccagaac ctcttccatg taagatcgtt
     4021 ccgcctgctg cacctgcttc ttttgaaaag tcgataacat cttcgcccat tcctccgtca
     4081 acgatcgtca caatcatgtg aagattcaat gctacaagat ctttcatttg ctttccccca
     4141 atttttgcaa tctttctttt tcgattctgt ctttttcatc ttttcgttta taaatatatc
     4201 ctagtactaa agtggataat attggtgtca atgcaatcat tgcaaccaca ccaaacccgt
     4261 caatgatcgg atctcttcct tcaatttgtg cagcgattgc cacgctcatt gataaaataa
     4321 atgtcgaaca catcgggccc gttgctactc caccgttatc aaaagccatt gctaaaaaga
     4381 tcttgtccac ttttcgtcct aaaataaatg ctagcccgta tcctgggatc aagaaataat
     4441 aaagagagaa tccgacgagc aaacgccaca tagacaatcc tactgctgcg ccgatcccaa
     4501 ttgaaattac catcagcatg attttcgatc ggattctccc ctcgctcaat tcttcaacct
     4561 gtttgatcat tacatgaatc gccggctcag caaaccctac taagaacccc attaaaaagc
     4621 caaggggaac cagtatataa ttgtaaggca atcccgcaat agcactgcct aaatgctgtc
     4681 ctaccggtac ataagccata ttgactccgt gcaggaaaag gatcaaccca atagtcgtta
     4741 tcaagaaacc tttcataatg tcaaagaaac gctttttgcg catacgaaaa ctaatgatat
     4801 tcattaaaac aaataggatt acgataggtg ccactgccaa aatgacttcc atggtaacga
     4861 tttcgattcc atctatcact tgctgccaga aactcattgg aagatcaccc ccaaaatcat
     4921 cacggcaaga atcggcccta acgaagcaat accgaccatt ccaaaactgt cattttcatt
     4981 tttcttttgt ttgatcatac tagtcatccc tccggcaagg gccaagataa atggaacagt
     5041 tactggacca gtagtcactc cccccgaatc aaaagcaatc ggcataaatt cggaactggt
     5101 gaaaaatgag gcgatcaaaa cgcctatgta gcctatagcc atcaattgat aataggatag
     5161 cttgaatacg acccgaagca gcgcaaaggc tagaaatact ccagtgccga cgctgacgat
     5221 cccaatcagc aacactctgc caatctctcc gtcagatata tcatatactt gttgtcccaa
     5281 aacttgcacg gaaggttcag caatcgtgat cacgatcccg atagcaaatc ccaaactgac
     5341 caaaacggtc aaacttttct ttttgatcat atatttccct actagatctc cgacttccat
     5401 catagaataa tctgctccaa acaaaaagag cgtcattccg atcatcatga ttgccgcacc
     5461 aaccagaaat gaagcgaggt cttctccttc taacggtgca aaaacaaacg ttaaaatgac
     5521 aatcaggaca gtcatcggca atatagcagc aatcacttct ttaaaatttt ttagcatgta
     5581 tcctcctcct ttactcttta agtttacctt aaaatgaaag agttttcgag cacaaactta
     5641 aaacgttatc atttaatttt tctcaacata aaataacaca cgtcccaaaa gtatcttttt
     5701 acttggaaaa atgactttga aggaacgcgc ttattgtgtt tttttatttt acagagccga
     5761 tcattccttg aacgaattgc ttttgcaaaa caaaaaatac gatggcagtt ggtaaagtag
     5821 cgatgactaa tccagccatg atcattccat agtctggtgt ataggaagca cccatagcag
     5881 ataaaacaag cggaaccgtc cgtttatcag gagattgcaa agctactaag ggccataaat
     5941 aattgttcca gctactcatg aatgtgatga ttgcagccgc ggcataggta tttttagccg
     6001 tcggcatata cactctaaaa aaaatactta gttcttttag accgtctaat cgagcggctt
     6061 ctactagatc ttttggaaaa gatttaacat tttgtcgaaa gaagaagatc aaaaaagcag
     6121 tgctaacaga tggcaggatg acaaccaaga aactgttgat tcccaaaacc gttccgttca
     6181 attgtgaaaa cattcgatat aaagggatca tcaatgccgc aaaaggaacc atcatcgata
     6241 gcagtagaat attgaaaatt aggtcttttc gtttactttg ataaatctca aatccatatc
     6301 ctgccattga cgaaatcagc aatgcaaaca cagtggtcac caaagcgatc atcgctgaat
     6361 tccaaaggga acgagtaaaa ttcagctcat tggaaaacaa attttgaaaa ttgatgatca
     6421 agttactgcc aatctttatt tttcccgatg tgatatccac gggtgtattc gtcattccca
     6481 aaaccatcca gacaaaaggg aaaatggaaa taaatgatag gatgcttaat gctgtatact
     6541 ttaatactga cccaattgtc gaacgaagtt ctaatgcttg ttttttgacg ttggggcgtt
     6601 cgattgtttc tttttctacc atatttttca tcgactttct cctcctactt tcgtttggat
     6661 tatcgacaat atcacaataa aggaaacaat aacaaaggaa actgctgccg catatccaaa
     6721 attaggcgtg aatttataac tcaaattata gatgtattga gatagggtgg tcgttgcatt
     6781 tgctggtccc ccgccagtaa tattttgtac ttcatcaaaa agctgcaatg tcccgatcgt
     6841 cgaagtaatg gaagtaaaca aaatgattgg ttttaaattt gggatcgtca ctttgataaa
     6901 ctgctgccat ttagaagcac cgtcaataga tgctgcttca taaatctccg aatcaatatt
     6961 ttgcatgcct gaaaggaaaa aaatcatatt gtatccagtc caacgccaag tgatcgaaag
     7021 aatgatcaga actctcgccc aaattggatg agtcaaccag ccaatcggtg tgtcaatcat
     7081 accaatgccc aacaacaagt tgtttacaag gccagattga gaaaatagac ttttcatgat
     7141 cagagaataa ctgaccatcg aagtgataca tggaagaaag atagcggttc gaaacaaccc
     7201 tttatatttc aactttttat catttaaaag gttggaaata aaaagtgcta gaaacaacat
     7261 gatcggtact tgtacaagta aatacaggaa tgtattaaaa aaagcttttt taaaagttgc
     7321 atctgtcaac atccgctgat aattacctaa cccgttaaat gtcatattga cagggggacc
     7381 actttgaaaa gacgtgatca aagccaaaac catcggaata aaaaccatga ttgatatcaa
     7441 aatgactggc aagattaaaa aagcatgccc gtttttgaat aaatctttct gcttcattct
     7501 tttccctcca attctatcat tgattatcta catgaaaaga caagaaagac aatatctgta
     7561 aggcagacgt gatctttttt gtcttttcgt ttgtctgatg aaaaagcgaa gaaattcact
     7621 tttttaccac cgatcaatta gccaattgtg cttctacttg tttttgtgta tctgctagta
     7681 ccttgtctgt ctcttctcca ttgatgattc gatgaagagc ttcagctacg actgcttcaa
     7741 ttgcataggt ctcatgtccg taattcactt ctggtatttc agatgtccat gttgaaaaat
     7801 cgtcaaagat cttttgacca ctgtaaaatt cagaagcttc ttgataagct tcttgttcat
     7861 tcgcgctaag catcgtagat actagtccga tttctttcgc aagagtgccc atcagttctt
     7921 cattcgaagc aaacgttgct tctaggaaat ctttcgctga atcttctcca gctactcctt
     7981 tgatcacata ccagccagcg ccgccaaggt tagaagcttg cgctttttgc agattttctg
     8041 gaagtgcagg tattggtgca atcttccatt ttccagattg gttttctgct ccttggatcg
     8101 ttgaagaata ccaagcgcca gttgggctag aagctactgc tcctgattgt atcgcagtta
     8161 ctccagcatt ccagtcagag gtttgttcga ctagatcttc tttcaaaaga gtggcaaaca
     8221 attcaagacc atactttaaa gatgcattgt cttggatcgt gactgtctcc ccatcttcag
     8281 atgtatacca ttctcctgcc tgttgcataa tcatccttac tcgaccaagg tcagaaggat
     8341 tgacttcagc gattttcttt cccgtctttt ctttgacatc tcgcgctact tgaatatact
     8401 cgtcccactt cagattctcc atatcctctt ctgtataacc tgcttctgcc atcagatccg
     8461 tacgatagaa gtttgctgtt acgcccgaat cgaaaggaac accgtatatt ttatctccta
     8521 ctttgtttac cgcaaattta tacggtgcaa aatgatcttc ctttacaata gcagacaaat
     8581 tagcgaatgc atctggataa gaagtcaaat agccttgtat cctataatct tcaatcaaga
     8641 cgatattggg caatccttct gtattcccgc tcgctaaagc ggtgttcagt ttctgaacga
     8701 tatcatcctg agacatcgta acaacttcta tctctgtttc ttcgttctca taaacttttt
     8761 ttgcttcatt cactgctttg atattgaatg tctcatccca cgcccagaca gtgacttttt
     8821 cttctttgtc tgctgtacta ctagcacttc cgcatcctgt taagaaacct gatgctgccg
     8881 ctaaacaaac gactccagca attaactttc tgatgttcat ttttatccgc tccttttcca
     8941 tccattagtt gtttacgttt gctacattca tcataccaaa attcgaaaac ggtttcttta
     9001 ttattcagat acttcaaaaa tttctcctaa aataataaaa aaatacaaat caactatgac
     9061 aaaaggaata atcttagcat ctagaccaaa gatcgaaatg gtgaggtttc tttaaaataa
     9121 aaatatcctt gcattttctc ctagaaataa gtatttttaa attacaagac tattcttaag
     9181 gcgggtaaaa aaatgccaat acgcgtaatt cttatcgatg atgaacagac gattttagat
     9241 ggaatgaaac agttgatcaa ttggtctgag cacgggtttc aattaatagg gactttttcc
     9301 agtccgctga aagcgcttga tttttctttg gtacatgcaa tcgatatcgt ggtcacagat
     9361 gtaatgatgc ctgaattaaa tggaatcgaa ttatcgcggc tattaaaaca gcaacaacct
     9421 gaaacacaga tccttatcct ctctagctat gatgaatttg atatggttaa agattctttt
     9481 aaagaaggcg tcgctgatta tcttttaaag cctcgattga gtcctgattt ttttctaaat
     9541 tccttaactg ccgtttcaca aaaaatcaaa aaaaagccca acaaaagccc aatcaccaat
     9601 cgttatgagc agatctgcga gaatctttca cagcagattg ctggacaaac tcgtccattg
     9661 cttgaccctc ttctttttga acacaccttg tttttctgtt atataccgaa aaacggccat
     9721 tcagttcaaa acaaggctct aaacaaaaac agtcgatcac ccattcattg atggatagcg
     9781 acgattcgtt tactatttat cctgtttcta ccttttcgga agaatccggc tatgtaatca
     9841 atactgattc caaagataca cttaggaagt ttgttcaaaa gtttcgtcaa gaagtttttc
     9901 caggtgattt ttttgttgtt tctgattctt ttgcaatcca agatatcaat gaaatattcc
     9961 aagagatcag aaattgcacc aaaggacaac gtttctatca taagggaaag aaccttgctt
    10021 atcagcatga acttcttccg ttattaacca accacgagca gcaagcaagt agttatttaa
    10081 caaaaatcgt tcataaagac tacgctttag ctgtcaaaga acttgagaat ttcttcacga
    10141 cgatattgga acagcctatt gatccagctt atttgaaaca tgaggcaatc aatcgtttct
    10201 atgcattact caacgctttg gctgacgagc atgttgggaa tgaagaactt tctttgctca
    10261 agatcgccct tccttcttta ttagccgatg ctgagtattt cgaagatttt tctattcttg
    10321 tcaaagaaac acttgagaaa ctcacccact tttctgcaaa tgcaaaaaaa gacaatttcg
    10381 atatcttgac tttgatctac gagttcgtcc aggaaaatta tgagaaagaa atcaatttac
    10441 aacttttatc cgaaaaatat catttttcat atagctatct ctcagccatc tttactgaaa
    10501 aatttggtat caacttttca aaatatctaa aaaaagtccg tatcagtaaa gccaagctct
    10561 ttttgacgga aacacggctt actttgacgg aaatctgtga gaaagtcggc tataccgaac
    10621 tgggttattt ctccagagtt tttaaagaag agacaggaat gacaccttct caataccgta
    10681 aaggaacttt tgtaaaatga gaaaaaaaat caaagagcat gaactctttg ccaaacttct
    10741 gttcattttg ttcatcggtc tttttctgca agccatcgtc atttctttgt ttatttatca
    10801 tcgatcaagg gatgcctata tccagctgtt caatcaatcg aatgatgtgg tactaaaaaa
    10861 gattcaatct gaatttgaaa gtttgaatga tacaatcgag aatacattag ccgcttttga
    10921 ttctaatcca gctgtcaaaa gctatttttc caatgatcca gcccaacata tggaacagct
    10981 tcagcaacta agaacaatcc aaaaaatgaa tgactctctt tcaaaaatcc atccaatgat
    11041 cgattacgat gtattgatct ttggagaaaa tggaagaact tttgtcggaa acgacatgct
    11101 cactgccgta tctgccgatt ctttttttca atctgcgatt gctcagagag tgaatgaacg
    11161 tgctgctgat acacaaatgc tttttgcaca ccacggattg actcttcgcg ataagaaagc
    11221 acccagcgtc ttctttgtca aaaaactgaa aaatacattg aatcacgttt acggatatgc
    11281 tgttttatcg atctcatcca agcaactagc gaatcttttt caatctgtag taaatcctga
    11341 gatatcgaag atctccttca tgaacgaaga acagacgatc atcgcatcaa atgaagaaaa
    11401 tacaattggg agccgatcaa tgattttcaa aaaactaaca gtaggtgaaa caacgatttc
    11461 aaatggacag cagttgaccc gtcttcctct gtaccgccag gattgtacat tactcagtca
    11521 agtcgatctt cattctttat ctaaccaaat gggcgtgatt tttccgatta ttttgtataa
    11581 tatcgtgaca cttgcttttg ctggctcatt ggtatttatg tatttggatc gacacacaaa
    11641 atcgatctac cgcttgattg attcgctaaa acggctagag gaagatggct taaaagctac
    11701 tgttcctgta caaggaagcc atgaagtcca attgctaggg aatacgatca atcaattgct
    11761 aagtaatgct cagcatcatt atgaacagac gatccaagac gaaaagaaga aacgttcgct
    11821 tgaaatccaa actatgcaag cacagataca gccccatttt atttataata cgctgactag
    11881 cttgaaattt ttgatttggc agaaaaaaac agatgaagca gttcaaggaa tcgacagttt
    11941 cattacttta ctaagaagta cgattgggaa aaaagaagaa gtgattccag tcaaggaaga
    12001 aatcaaaagc gtacaaagtt acatcgatct tttgagtatg cgatatggcg aaagaatctc
    12061 ggctaaaata atgattcctg atgaactgca gtcattatct atgccgaata tgatccttca
    12121 accaattatc gaaaacgcct atcttcatgc ttttcacaat aaaacgcaag gattcatcac
    12181 ggtgtacggc aaaacaaaaa atcagcttct tttattcgaa gtgattgata acggcgatgg
    12241 cttcgatact gaaaatacca aacaaaaaaa tgatcatttt tcaggaatcg gcatcagcca
    12301 tgtagatgag cggatacgcc taatgtatgg cgatgcatat ggcttaacga tccaatcagt
    12361 gattgggaca ggaacgactg taacgatttg tctaccgctt cttcctaaaa atcaatcaga
    12421 atatcaaaaa taaagaatag ccaagcgctg atttttttat tttaagttaa ataaatcaat
    12481 cagtattctt gactttcatc tacgcgtctc ttctactgtc agcactggat aacagcccaa
    12541 tatttgactt tctcctccga gcatcttgat ttcttggagc gcattctcta ctaatgtcat
    12601 tggacggtcc aacaacaaat cgatcacgaa aaaatattct cccaagctag ttttcaatgg
    12661 tcgggattca atcttactta agttgatttc ccgccagcca aaagctgcca gcattttatg
    12721 aagcatccct ggccgattag caggtagtgt caagatgaca ctcattttta ctggagctga
    12781 ctgctggctg gtcatctttc ggtcaccgac gatccaaaaa cgtgtctggt tcaactcatt
    12841 atcttggata ttctcagcta agatctccaa tccaacatgc tgtgctgttt ctaatgaagc
    12901 aatcgctgct gcgtcttctt tcggatgttc tgctacatac attgctgcag cagttgttga
    12961 ttctgttgct actaaaggaa cattaggata atgcgtttct aaaaactgtt gtgattgtgc
    13021 tagtgcttgt ggatgtgaaa ggatttttgt aatctttgtt gctggattgc ctaaaagctg
    13081 ctgcttgata ggaagaacga tttcactttt aacttcgact tctggatgtt tggaaagaag
    13141 atcgattgtg tgatgaacag ctccttccaa tgaattctct acaggaacga cagccagatc
    13201 gacttgtttg cgaaacaaag cgcgcaaaca aacagggatg gaagcatacg cagtcaattg
    13261 ttctgtagga aatagctgac aagccgcctg ataagtgaat gaactttctg gccctagata
    13321 gctgacgttc atatgctttt gatcctttct ataatggtat cgatgatctt ttctggtgtt
    13381 tgattggtcg tgttgattcg atgtgtcgca ctttcttcat accaagaaag tcgttgtgcc
    13441 aataatgctt ttatttcttc tcttgttttt ccaagagcta aaggtcgaat agacgtttca
    13501 tcttgttgga tacgatcaac tagacagtcc gcttctgcct ctaagtaaag aacaatcggc
    13561 tgtgttttta aaaaacgccg attttctgca ctttggacaa tccctccacc agtcgcgata
    13621 attttgtctt catttgacag ctttcttaaa atatctgact ccacttttcg aaagcttttt
    13681 tcaccgtaat agtgaaaata ttcggcaatc gacaattgga tatgttcctc aatctttgta
    13741 tctaaatcta catactcttt ttgtaaacat tgagctaacc ctttaccgat tgttgttttt
    13801 ccagcgccca tgaaaccaat cagcaaaatg ctagccatga gcttctcctc ctgacacaag
    13861 ttctgctaaa tcgtcaaaaa atgccggata agagattgaa acagcttcag ctccttctag
    13921 ttcgcaaggt ccatcagcta tcaaagcagc tacttgcagc atcataccga ttcgatgatc
    13981 gcctctgctg gacacttttg cgccatgcag aggtgtcggc ccatggatga tcagaccgtc
    14041 tgctgtcgct tttatctttg ctcccatttt ctgtaattct tctgccaccg catcaattct
    14101 gttggtttct tttactttta attcttctgc gtctttgatg atcgtgattc cttctgcttg
    14161 agtagccact aaagctagaa tgggcaattc atcaatcaga cgcggaatga tttctccacc
    14221 aatcgttgct ttcttcaaat gactcgtctt gacacttaaa tcagccgatt ggttctgttc
    14281 attgtgattc gtctgggtaa tcgctgcgcc catctcctcc aacacatcta agataccagt
    14341 tcttgttgga ttgaccccga cattttttaa caacagttgg ctttccggaa caagaagtcc
    14401 tgctgctaga aagaaagcag ctgatgaaat atcacctgga acagttatct gttgaccagt
    14461 taatttttgc ggtccggtca ctatgatcgt tttatcttcc actgtgatcc ttccgccaaa
    14521 ttgacgaatc atttcttccg tatggttacg ggaacgttct ttttcaacga ttacggatgt
    14581 gccttctgct tgtaatgccg caaacagcaa agcagatttg acttgcgcac tggctacagg
    14641 catattgtat cggataggtg ataaagagcg ggttcctgtc acagtgattg gtagatactg
    14701 gtcattttct tctccttgca agtcagcgcc catctcgcgt aaaggttcca tcactcgccc
    14761 cattggacgt ttagacaacg aagcatcccc gaacagagta gtcgtaaaag gctgtccagc
    14821 caaaataccc atcaataaac gtatggtcgt accggagttt cccatgtcaa ttgattcggt
    14881 tggttggaca aatgagtggc taccgcgacc atgaatcttg atcacctctt cctcttcttc
    14941 gatctcgaca cctaattgtt ggaaggcatg taaagtactc agacaatctt ctgcgcgcaa
    15001 aaaattttgt atagtcgtcg ttccttcagc aattgctcca aacatgatgc tgcgatggga
    15061 aattgattta tctgctggta tcctaacagt ccctcttaat ccatgtattt gttgtaataa
    15121 ttgcatttat ccagcctcct atttgatcca gcaatgataa ctggtattag acataatgat
    15181 tctttttgct tgtaacaaat cctgttggtt tttaaaactg atttgcagta ttcctaaaat
    15241 atcttccctg gtctcttgga tcttcaaatt aatcaaagaa actcccgctt ttccgatcag
    15301 tccagttact tctgcaataa cgccaggttc atctggcaca tcgataaata aatcaaaaaa
    15361 ggcaggaatc gcgccttgct tatggacagg caactggtct cgactttctt ttgcccgatt
    15421 gaaaaaagaa aagatttccg attggttctc agtcatgatc cattctgcta tttgtgtcat
    15481 ttcttgttgc caatctgcca atcgttccaa taatatctgc ttattgctca acaaaatatc
    15541 tgtccacatg acaggatctg aggaagcgat cctagtgata tctcgaaagc ctcctgctgc
    15601 cagttgtttg gctcgcggat actcttgatt gaattgttct gcctgattga ctaagccagc
    15661 tgccagaata tgtggaaaat gacttagcat tcctgttata cggtcatgtt ctcttgctgt
    15721 aagagttaca tattttgctt tggttccgca aaatagttct tgcagttttt caacatcttt
    15781 tttcgtcttt tccaaaggtg taaagatgta ataggcattt tcaaatagtt tagcatctgc
    15841 tgcctgtata cctgatttat gagaaccagc cattggatgt ccaccgacaa accgaagatt
    15901 tttctgctca gcggatgtca cgacttgctg cttcgtactg ctgacatccg taatgatgac
    15961 gtgatcagac aagttgattc tttcaaggac atcaagataa gctaaggttg tacggacggg
    16021 aacggctaat aagataacgt ctgcttgttc tgcacctgtt tcaaaatcac ttgcaacgta
    16081 atcaacgatc tgctgttcta ctgcttgctc acgggttttt gactgactat cccaaccaac
    16141 tactatcagt tctggattgg tctgacgaat acaaagtgcc atagaagcac caatcagtcc
    16201 aagccccacg ataaaaacat gttgtgccat cttatttgcc ccctttttag taatgtttca
    16261 agtagtcacg gtactccttc actgcagctt tcaactcaac aagcgaatca cttgggaatt
    16321 ttgacaatag ctctgtcgct accacagtgg ctacaacatt ttccgcaacg acgctagctg
    16381 caggaactgc tgtactatcc gaacgttcta cacttgcttt gtaaggttct tttgtatcga
    16441 tatcaacact ttgaagtggc ttgtacaatg taggaatggg tttcatgacc ccgcgaatga
    16501 caagtggttc gccattggtc attccgcctt cgaagccgcc tagattattg gtacgtcgga
    16561 aatagcctct ttcttcttcc cataggatct catccatcac ttctgagcct ggtaatgaac
    16621 cagaagtgaa tccgacccca aattctacgc ctttaaaggc attgatacta accacagctt
    16681 gtgcgatttt ggcatctagc ttcgtgtccc attgtacgta gcttcctaat ccagctggaa
    16741 cgcctccaac gagcacttct acaattcctc cgatcgtatc tccatttttc tttgtctgat
    16801 cgatcaaatc ttttattctt tgctcaactg ctggatcaac aactcgtact tctgacgctt
    16861 cagaaatttc tttgatttct tgaatcgaga tatttccagg tatttctgct tggattccac
    16921 caagacaaat cacgtgtcct gctacgatga tgtcaagttc ttccagaatt ttcttggcaa
    16981 ttgcgccgat tgctacacgc atggtcgtct ctcttgcaga ggaccgttca agcacattcc
    17041 gaagatctcg atgatggtat ttcataccgc ctaccaaatc cgcgtgtcct ggacgcggac
    17101 gtgcaactcg ccgcagtgat tggtcttttt caggcacttc tgctacagac atgacagttg
    17161 tccaattttt ccaatcttta ttttctacca ctaaagtgac aggtgaacca attgttttgc
    17221 cgtgacggat gccagacgtg atccgaacac gatctttttc gatcttcatc cttcctcctc
    17281 tgccgtaacc agcttgtctg cgagcgagtt cgtgattgat ttcttcttct gacagaggta
    17341 atcctgctgg tagtccttca ataatggctg ttaattctgg tccatgggat tctcccgcag
    17401 tcatataacg catcttacag tcctcctaag taatctttga atgtctctgt gggaacagga
    17461 acgatttttg cttgcccaat cttttctagc aagatgatat tcaattggct tcctcttgct
    17521 ttcttatcat gggttatcgc gtggaataat tcatctggat gacttagatc gtacgtaatc
    17581 ggcagatgga atttttgaag tatcctttct aaagcagctg tcgatccagt tggagacaag
    17641 ccgatttttt ctgcatgaga agtgatggct accattccaa tagctactcc ttctccatga
    17701 ctgactaccc catatcctgc tgtattttca attgcatggc caattgtatg accaaaattt
    17761 aaaataagtc gatttccttg gtcaaagaca tcttcttcca ctacttttcg tttgatttcc
    17821 aacgtagcag taatgattgg aacagcatgt tccagaagat ctttttcgtc tttcagctcg
    17881 ttcagctgtt tccaaagcgt tgcatctgct atagctgctg atttgatgat ttcagcgatt
    17941 ccttctcgta aacgacgtac cggcaaagtt tttagaacct caggatcaat caatacacca
    18001 gctggctggc taaatgttcc gacaaggttt ttcgctttgt ttgtattgac tgctgtcttc
    18061 ccgccaatac tagaatctac ttgtgcaagt agcgtagtcg gaatttgaac gaaatggatc
    18121 ccacgcatga acgtcgaggc cacgaatgct gctagatcac ccaccattcc tccaccaagt
    18181 gcaagtatcc catcgctgcg ggtaaaatca ttatctgcta aaaaatcgta taactcagct
    18241 gcttgttcta aagatttact ggtctcaccc gccggtacga ttgaaaggac tggttcaaaa
    18301 ttagcttgtc tcaattggaa catgattttt tctgcatata gtggagcaac gtttgtatca
    18361 gtgatcacag ctactttttg aggtctccaa atcgtttgaa gccatgatcc agcctgttct
    18421 agtgcgcctt tttcaatatg aatatcgtat ttttggttat caacatgtac agtaagcatt
    18481 cgtttcactt ctttctttta aaaagctgga attaatcgag tgtctgaatc ttttccattg
    18541 cttctttcat gatcgcaact tctttcatca tgcgtgagta agtcttttca ttcaacgatt
    18601 ggggaccgtc tgaccaagca tttgcaggat caggatggat ctctacgatc atgccatctg
    18661 ctccgcttgc cacacccgca cgagccattg gtggtacaag ttcccaaatt cctgtaccat
    18721 gacttggatc aacaatcaca gggaaatgag ttaatttttt catcaatggg acagcactta
    18781 aatcaaacgt attacgtgtc gctgtttcgt aagtccgaat cccacgttcg ataaaaatga
    18841 caggagattt gtcttcgact gcaatgtatt ctgcagcatt aagccattca ttgatcgtac
    18901 cggaaattcc acgtttcaat ccaacaggtt tacctgtttt accgacagca gatagcagtt
    18961 taaaattttg catattacgt gcgccaatct gtaggatatc gctgtactca gccaccatat
    19021 cgatatgccc ttcatccatc acttcagtaa tcactttcat tccaaattca tcagccgctt
    19081 gacggatgta tttcaatcct tcttcttcta aaccttggaa agcatatgga gaagtacgtg
    19141 gtttgaaggc accacctcgc agaatttttg cgcccccggc tttggccatt cgcgcacatt
    19201 ctctgatttg gtctaatcct tcgatagaac aaggaccagc catcgtcacg aaactgccgt
    19261 caccaatttt tactccgtct acatccacga ctgtattgcc aggatggaat tctcgagaag
    19321 tcaatttgta tgtgtttaaa atacgcacag cattttctac tccttcatag cgtgtaaatg
    19381 ctccttcttg taaacttcgc gtatcacctt ttagtccaat cactactcgt tctgttcctt
    19441 gatcgattga gactttgaat ccttcttttt cgatatgatc gacgactttt tttatttgtt
    19501 cttccgttgc ttcttttttc ataattacta tcattgtttt tcccgctttc ttttgtttac
    19561 ttatctatct tggctgattt tctctacttt ttcaaggtgg ttttacatta attcctacta
    19621 aatgaccgtg tcctgttgtt ctcattttcg ctccttcttt cctacttatg acccagataa
    19681 tcaacaaaaa aagatgtccc tgtaggaaat tgcctctaca gtgaacatct tcaacgagcc
    19741 ttaaaaagcg ctgcaaaaat ataaaatagc cactatagat gaatttctct acgtggctgt
    19801 aagtcgtgtt taaaaatctt tagccatcat agatacaaac ctggtttgtt tgtacccacg
    19861 aagtcatgaa tacaaaccta tctaaaatag ctaaagtaat acgtattcag ttttgtaagt
    19921 tttctagatt tcataaaaaa tcgctccaaa ctgatgtaag aattgtacct agtatagaca
    19981 taaaaaaatc gactgtcaat catttttatg aaactttttt taattttctg atagttaact
    20041 ttgtttttca gaaaaataac aattggtttt attgaagaaa atagtcatga acaaaagtat
    20101 ctgagaactt ttttactctc ttgttcatga catatagaat aaagcagctt tgtcttgtaa
    20161 actccgtttt tcaaataata atctaaagat tgctactgaa taataaagcc gatttcatgg
    20221 tcctcttcgc tgctgattcg gtaaggtgac tcaacatccg ctccccagct attgataccc
    20281 ccgactcctc ttacttttgc aaaaatcgta agaaccgttc gtctttctgg cggtagttct
    20341 tctatatgtg ttgcctgttc cagttcttct gctttgaatg gtaaagcgga aaaagcaaat
    20401 ggcgtatcct gctgttcaaa tcgcaacgaa aaatcttctt gatctttatc cgtattatcc
    20461 aacgtttgat ttcttgttag ttctacccat ttcgtttcca tatgcatgcc gcattcttgg
    20521 ggaacaaggt atggggaaac ggggagtcct gccactttat aaataccttc gacaccacca
    20581 gccatccgat ccggatatgt ttcccctgat aggccttcat actcaaaaaa ttttgctttc
    20641 gttggcataa tgaaccttaa acccaatgcg ggtaattccg gtaatccttt ttgtccataa
    20701 taatgcatat tgacaagcat tttaccctct ccagcgattt ggtaggaaat tgtcacttgg
    20761 gtttttggta ctgtgttgat ttccagtgta aaaatgattt caaccgtttc agcgtattca
    20821 ttacttgaat attgattatt tactggagca atcggcaaat cgatcttttt attgtccacc
    20881 agtacttcta tttctttgac ttttggatac aatccggctc ctaaccacat accggattta
    20941 aatccaaatc cgctacctct gtcattatcg gtcgttgctc gccaaaaaac tggaagtgtt
    21001 tcccggtata gccattcttt tccgttttta ttgagcgatt ccagtccgcc tcttgtataa
    21061 gaaaaaatat aatgcgtatt ccccgatttg acacctaatg tacagtcgcc gaagacgatt
    21121 gcaacttttt ccgtatccat atgactttta tttactgtat ttttcataat aatatcttac
    21181 ctcctgcatg gcgggttttt cttgtccatc tgcaaaaacg atcccattcc ctgagaattc
    21241 atagtcactt ggtttatcgt caaagtcacc gccatagcgc atcacttctc tgcctgtgat
    21301 tggatcagtc acttggatcg cctgatcttt aaaatcccat atatagcctc cttggtacat
    21361 ttcataacga tcaagcaacg tcatgtaaga atccattcca cctaacgaat tccccatgtc
    21421 atgcatatat tcacataaga tgaacggttt ttccgcttta ttttcaaggt agtcaatgat
    21481 ctcttctggt ggcgcataca tgcagctttt tacatctgaa atcgaatctt cgaatgctgg
    21541 atttggaaaa ttcccttcat aatggacaag tcgatcgggg tctacttttt tgaagtaatc
    21601 gttcatttgt cgaatgtttt cttccgcata agattcattt ccaagagacc aaaaaagaat
    21661 acttggatgg ttttttaatg tttcgaaatt cgtttttgct cgatcaagaa cagccgcctg
    21721 ccactgaggt actgcacctg gtacgttgta agaaggctca aatgcgccca ttttttgcca
    21781 tgagccatgg gattcgaggt tcgtttccgc catcacatgg attcctgctt ggtcgcataa
    21841 ataataccaa gtgatctggt tggggtaatg agatgtccgt acgccgttga tctttgcatt
    21901 ttttatgata gccaaatctt tttccatatc ttctatagtg atggcgcgtc cttttttcgg
    21961 atgccactca tgtcgattta ccccgttgat aatcatccgt tttccattga agtagatgat
    22021 tttatctttg atttcgattt tacgaaaacc gaaggaatag tcaatatact ccgttactct
    22081 tttttcttcg tctatcgtct ccaattcaag cttatataaa ttgggctgaa aatgccccca
    22141 cagtgccaca ttttcaaatc catcgatgcg aatcgtcatt tgctctttta acggatattg
    22201 ctgttgatag atttccttcc catcaggatc tagtatcgct acattcagct gactgttttt
    22261 ggctacttgc tcaaaagaca aatctacttc aagtttgcca ttttgacatt tgtcatctaa
    22321 gcgaggtttg atccaaagat cttctaaatg gttttgtcct cgagcaacta agaagacatc
    22381 tctgaagatc ccaaaaaatc ggaaaaagtc ctgatcttcc aagaatgcag ctgtacttct
    22441 tttgtaaacc tccacagcta gaatattttc ttcttccaaa taaggtgtta aatcaaattc
    22501 agacggagtg aacgaatctt cggcataccc gataaaatga ccattcaacc aaataaacat
    22561 tgcttgttct acaccagcaa aatgaatcga gatttgtttt ccctttagac cttcttctaa
    22621 tcgaaaagtc gtcaaatacg aaccaaccgg attgtcttta gcagctgaaa agatcccttc
    22681 cgcctctttc tcagggcttg tcgtgaaagg gggacggcga aatacatgtc cctcccacgg
    22741 atacatcgta ttgatgtaat ggatcttgtc ataatttgct aattcaatat gactaggaac
    22801 agtgatttca tcaaaagaac aggcatcaaa atctttttta taaaatgttt ttggtcgtac
    22861 cgaaggattt ggcgaataac aaaaacgcca tgatccattt aaagactgtt taagggagga
    22921 tgtggtttct acttcttccc ttgttgcata acaaacatga tcgctatgtg catttgattg
    22981 gttcactcta aaaacttgtg gatcatctaa ccattttata tttgctttca tacttttttc
    23041 cttctctctc ataacttttt gaaatgaagc agttctgttt ggaaatcacc ttgaaaatcg
    23101 gggaagtaaa gtccaactgt catcaattcg gcccccgaaa atatccgctt cgaggtctgt
    23161 tcctgataaa gtgcctcatc atctaacccc atcagatata cttcatggaa cgctggttga
    23221 gctgaagcta gtatcctccc taaaaacaca atagcttcat cttgtttagg agacacaaac
    23281 atccatgcgg caatattttc ttcaaaagga ctcgccaaac gataataatg gccatactgg
    23341 atcaccggac gaatcgtttg ataggatata atttggtctt ttacttgctt ttcttcttca
    23401 ggagacagct ttgtcaaatc cagttcataa ccaaaaacag cgctcatagc tgtttctcct
    23461 cttgttttca acgaagtaga tctgtcggtc tgatgatttg gtacagctga tacatgagct
    23521 gtgatggacg aaatcggata agcaaggctt gttccatatt ggattttcat acgctccact
    23581 gcatccgtgt tatcacttgt ccatgactgc ggcatataat aaataaaccc ggcatcaaac
    23641 cgtccacctc caccagaaca gccttcccat aagatttccg gataatcagt tgtcaaatgt
    23701 tcaagtagtt cataaagccc gagcatataa cgatgcgcta cttctccttg tctctgtggg
    23761 tcaagggtaa tcgaatagac atctgaaagc gaacggttca tatcccattt gatgtagtca
    23821 agaggaatcg tatctaaaat ctttctcatt tgtttctcaa tcgtctgccg aacatctaac
    23881 cttgtaaaat ccagtacatg ttgtgatctg gaagcagaag gcattctacc tggctcctgc
    23941 ataagaaaat caggatgtgt tcgatacagt tcagaatcaa ccgagatcat ttcaggttca
    24001 aaccataaac caaatttcaa tcctttgcta tgagcataat ctgctatttc acgtaatcca
    24061 tttgtcaact tgccttcata ctcgaaccag tctcctaatg aagaattgtc cgcatctctt
    24121 tttccaaacc agccatcatc taatacgaac atttcgatgc ctagctcctt tgcttcatca
    24181 atgatcgcct tgattttttc actattgaag tcaaagtaag tggcttccca gttattgacc
    24241 agaatcggcc gttcagcaaa ttgatgacga cctcttgcga ttctttcacg tagcaactca
    24301 tggtggatct gactcatttt gttcaatccc tgacttgagt aagaaaggat catttcaggt
    24361 gtttgaaaag actggcctgc attcaattcc cacgaaaaac gatggctgtt gatcccaccg
    24421 acgactctca attgattgat ctgatctttt tcaagttcga aagaatggtt gccggaataa
    24481 acaaattgaa gtccgatcgc ttctccagta ttttctgtcg tatgatgatg aactaaagca
    24541 ataaagttat tcatatggtg actgctcgtt cctcgacggc tttcaaatac ttttgttcca
    24601 gataaaacag attgccgtga aatctgtctt tcattgacat gtgctcctgg caaagcaatg
    24661 acttcatcaa atcgcctagt atgggcaaag tctagctgga aactagccgc tttttctagt
    24721 ttgattgttt ctccggtttc atttcttatc tttacagatc gtgtgatcac tgctctatca
    24781 gtaaatatgg tatagaacaa atcgaaataa atcgctgttt ctctatcctt aagtgaaatc
    24841 accaacgttt gtgcctctga cttgtccacc acataagttt gaggcaagcc ttcgatctct
    24901 ggcttcccat ctgtaatata atggctgtca tatctcagat ccagtaagtc tgatccattc
    24961 ttgcgctgaa tgatcgatgc gggcgttcga aagtccatgc tgccatgaga agagtattct
    25021 tgcggtagtg tatctttcga cagagaccgg tcaggattta agggaacatt tcctgaaaat
    25081 ccacgatctc ttcggggata atttttattg tctttgtact gcttgactgg tttgccaaaa
    25141 taaatatgcg ccaatacatt gctttcttct atctttatca gataagaaag aaatgtattt
    25201 cgtaaatgga agactcggct cttttcatca aataagatga aatttttatc tgtttttcta
    25261 tgcgtaatat ccatcatttc actcccaaca aaacggaaac cgtttacttt tttttataat
    25321 tcctgtatac tgtttactaa acaattttgt caataaaaat tttttatttt tttgaggagt
    25381 gtgtaaaaat ggttggaatt agagatgtcg cacgaaaggc aaatgtctct cctgctacag
    25441 tttcccgtgt gttgaataac gatcagacga tccatgtggc tcagactact cggcaacgga
    25501 tagtagaggc agctgctgcg ttggattatg acatcagtac tcgtaaatat atcaaaaaga
    25561 gaatgccttc gattggtgtg atctctacga tcggcaaagc gagcgaagaa caggatatct
    25621 attacaaaca tttgcgagta gggatggaag aagaagctcg acgtctgcat ttagggatga
    25681 atcgaatcta taatctatct gataatccta aacaatggga agatctagat cagttaggtg
    25741 cggtgatcgt tgttgggact gttacgaaag aatcgattac cgccttgtta aaacaaaaca
    25801 tgcaaatagt cgtcgtggat aaccctgaca tccagcaaga ggtcgatatg gtgtatgcag
    25861 atcttgaacg gatgactaaa accgtactgg aattgttctt agaaaatggt cataaaagaa
    25921 tagcctatat cgggggatat aaagtcgata tggatgaatt cggtgggaag cagatttcta
    25981 taaatgaaaa gaggatgcgt gcctatcagc attttatgat cgatcatcac ttagaaaaat
    26041 atatgtctta tcatctaggt ggctggacag aaaaacaagg tgaggtactt gcgactaatc
    26101 tattagaaaa tgaatcccct cttcctactg ccattcttgt cgggagcgat ccaatggcaa
    26161 ttggtgtcta tcgggcattg aaacaaaaaa atatccaaat tggagaagag attgttgtag
    26221 caagctttga taatatcgag acaatcgcaa acctctcccc tggcttgacc agtgttcaaa
    26281 tcaatgcgaa atcaattggg caagcagctg ttcgaatggc attggagcga attgacggta
    26341 tacgggacga accaatcatt ttgacttatc ctgcacgatt gattgttagg gaaagtttta
    26401 tcccaagaac aaaaaggtat tgaaagagag gttgtgaaaa gccgcttcgt ttcttcatac
    26461 tcaaaacgaa acggcttttt cacaacctct tacttatact tattcacaaa atccaatgtc
    26521 tgctggtaac aattttgcga ttcttttagc gtgtaatcaa attggtattc atgggtaatc
    26581 tctttttttg tatctgtata aaacaaggat tgcacgggta cctttaattc tgtcaaccga
    26641 ttttctaatg cttgaccttg ctcctggaat gagtatgcat ttccgtcagt tatatacgtt
    26701 ggcggaaatt ccttagaaac atggtcagcc acactagctt ctttcaattg ctcactcgtt
    26761 ttccaatctt ttgttccaat caacgaccac gcaacggttt tgacgaaaaa cttcataaat
    26821 cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa caggtccgca ataagaaatg
    26881 gttcctttca gactatcttt tggaagtagc tgtttgattt tcatttgttt agcatacgca
    26941 tcattcgttt gtatagctgc atattgcaat gcgatttgtg aacctgcact atctccacca
    27001 aaaaataatt gttccatgtc tagcaatttg tcgtcttttt cttcagctaa taaatctttg
    27061 atcagttcat tcacttgaag aacttgatta gggtattctg aatcaggcgc taattcataa
    27121 ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt ttgtcgcaaa ttctttgaca
    27181 cccgatttat ctccgccgac gaatcctcca ccatgtaccc aaaatagaac aggaacaggc
    27241 cccttgctgt tttttggata gtagatgtca taagtattct ctttatactt ggactcatac
    27301 gtcaaatctg tttttttcgc cacgtttttt tctgcttgat cgaacgtttt tttatcagtg
    27361 atttgaacag tatttgaaaa catatggttg atcaagaatg ctccaggtct gggggaaagc
    27421 tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga ttccgaagaa ccatccgata
    27481 aatttaagaa ttttcttttt cataaggctc cctcagtagt tagtattaag cattctgatt
    27541 ataccatagt gttatttcct atcaagctct cctagatgat ttaatgattt tccccaaact
    27601 tcttctgcaa tgcttttgac aaaagaaatt ttttccattg ttcttcaaac aaaacatttt
    27661 cttcttctgt tgaagcaaat ccacattgag gacttaagca caaacgatcc aaaggcagat
    27721 atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg ctcttccatt tcgccatttt
    27781 tcgttacagt caaaatctaa gacaactaat ttatcttcag agactttctc taaaagaaca
    27841 aaactcccag cacgttcact atcgtattct aaataatagg cacggacatt ttcttgagta
    27901 aaataatggg tctgctacgt cgtcatcccc cctgatgccg ccaaaatcga ataataattt
    27961 ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga atatgaaaat aaaaatcttt
    28021 ttgatcaatc agttaatcaa attgaaataa cgaaaaatct gagacagaat gacgttcatt
    28081 ctgacccaga tttgattttg cacaactcaa cagtccttat tggcaaatcg ccaaaataat
    28141 gtagactaac taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt
    28201 tagcacgtct atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta
    28261 ggtgtttcca tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt
    28321 ttttcttttt ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat
    28381 gggtttgcgg gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa
    28441 ataacttagc atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg
    28501 gtgacatttc gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa
    28561 ctacgatcac agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt
    28621 ttccgacttc ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt
    28681 caattgcttc tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca
    28741 agccaatcgt acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg
    28801 caaactcgat ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg
    28861 actttgctga gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat
    28921 ataaaacttt ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga
    28981 tgaccgaaac cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa
    29041 agaatttttc tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac
    29101 ggataatgat gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga
    29161 accgttgcaa gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat
    29221 aaaaatcttc gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact
    29281 gtccatcgat acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca
    29341 ttccgaccgt tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct
    29401 ctacttgttt ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga
    29461 tacctatgcg aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa
    29521 atgtttcgac ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt
    29581 cttctccagg gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca
    29641 ttccttcata actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt
    29701 gctgttctgt catattttgc tcctttaagc ctatttttca atcctactga actctactat
    29761 aaatcagcta attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt
    29821 tgattaattc ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc
    29881 atccgttaaa cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc
    29941 atctgtacct gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg
    30001 ttgatgaaga ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat
    30061 cattactggt cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata
    30121 gattattctt ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa
    30181 gatctcaaag atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt
    30241 ggcatctggc gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat
    30301 cgaagcattt ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac
    30361 tcgagggagc atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag
    30421 ttctgatgga acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa
    30481 gtaactgtta tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc
    30541 tgctgctgct ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt
    30601 accagctggg atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac
    30661 ttcgttgatt gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac
    30721 tctacatgct tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc
    30781 aaatcctgct tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt
    30841 tcccgaggtg gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc
    30901 taattcatct ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt
    30961 catgatttga ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac
    31021 agattgttgg ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga
    31081 taattgcacc caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa
    31141 ttcacggaag acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat
    31201 cattcctgct aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt
    31261 caagtaagca gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt
    31321 ttccaaaaca gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc
    31381 atactctgga agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg
    31441 caaactagct tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg
    31501 ctctgggaag taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt
    31561 cgttgtttcg tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt
    31621 tgcttgacgt ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt
    31681 tttcaattct gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc
    31741 atcacagcgc atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat
    31801 tgatcgcaat gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga
    31861 aacgatttcg atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat
    31921 accatgcatg ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt
    31981 ctttttacct tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg
    32041 ggaaatttga tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt
    32101 atctttagag atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc
    32161 tttattcatt actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt
    32221 tggttctgca ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc
    32281 gacgtggact tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata
    32341 gctggttgtt gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc
    32401 gttttttcat caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa
    32461 cctgccggta cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca
    32521 ctcatgtaca ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt
    32581 gaagatggcc cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc
    32641 aatgtacgga cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag
    32701 aatgtaccta acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg
    32761 acatatacat catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg
    32821 aatcgttgca aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg
    32881 tattttgaat gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct
    32941 gctgctttca atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta
    33001 ggtaaagcaa tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact
    33061 gaaacgcctg aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat
    33121 gcattatctt tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca
    33181 atcaaaccaa aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa
    33241 gctgctggtt ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca
    33301 gctgcaactg ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa
    33361 gcattttttg ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc
    33421 atgtttagct taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca
    33481 tcataaattg gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt
    33541 gttacgatat tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc
    33601 cctttttcat ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat
    33661 gcctcgacct caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt
    33721 acagtgattt ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat
    33781 tctgtcatta tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt
    33841 cttctgtttc cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat
    33901 cttgtcgcat cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat
    33961 ctacttcttc cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg
    34021 caagttcttc atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt
    34081 cactaatcgc catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta
    34141 acacaagagt gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc
    34201 attcttctac tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt
    34261 cgattcctaa ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga
    34321 tatccgtttt tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt
    34381 ttgcttcttc acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa
    34441 aaggagactc gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc
    34501 ctgcttgttt taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta
    34561 cactgtcggc aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt
    34621 tactcaatgt ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga
    34681 catgtcggat tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga
    34741 ttgctgtcaa aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt
    34801 cttctgtcaa tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt
    34861 ctagtacgcg cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca
    34921 aaccttcttt catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat
    34981 ccaaatggac aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc
    35041 tgtctttagg tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt
    35101 tataaatcag aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat
    35161 gcaggctggc acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg
    35221 gcgttactac ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt
    35281 cttctggcgg aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt
    35341 gatcttgtag cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat
    35401 ccacacgttt gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata
    35461 gacgtttttc ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact
    35521 gggtttttgc ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc
    35581 ttttcgctgt gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat
    35641 tcgcaaagat atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt
    35701 ttggcatgaa acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga
    35761 ccgaccggac agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag
    35821 tcgcacctct cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt
    35881 ttagttcaca gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag
    35941 ctatcagctc ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga
    36001 ctttttcaaa accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga
    36061 tcaaatcagg atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat
    36121 agtcttccac tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg
    36181 tacgaagttc ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt
    36241 accctccact actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct
    36301 tgacttggga aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact
    36361 gttcctagac cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc
    36421 cctccagtag cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct
    36481 gtttttttag cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt
    36541 aactcctgat cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat
    36601 aatgttctgg agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca
    36661 tatgcgagac ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga
    36721 aaaaccccct cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca
    36781 tgcaaagtca tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca
    36841 agtgccagat catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca
    36901 ttttgctgtt cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt
    36961 tcgatgcgtg cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta
    37021 cgatccaaaa cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact
    37081 tcttggatca tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc
    37141 agatccacat tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt
    37201 agtttttcga tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa
    37261 ctgattgaat cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct
    37321 ttacgatcgt agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga
    37381 gtttcttcca ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa
    37441 ttccgatgat tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct
    37501 cgttcattat ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag
    37561 ttttttggtt ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt
    37621 ttcgtggagc gatagttttg ttctagcaaa attactgcag catctggata atctttttca
    37681 aaatccaaga tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct
    37741 cccacgacgc acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg
    37801 tgatttgtat cttggtactc atctacatga atataatgga atttgttttg ataatacgtg
    37861 agtgaatcag gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc
    37921 atacattgat tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct
    37981 tcgtaataac tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct
    38041 tgagagattg ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct
    38101 tttagtatcc gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg
    38161 ttatacccga taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc
    38221 gatacccaga catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct
    38281 tttgcagcct tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc
    38341 tcaatcaaat aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc
    38401 attagcagta atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg
    38461 tttaataatt cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat
    38521 tatagcattt tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat
    38581 ggatttttcc ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga
    38641 atggaaaaaa aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa
    38701 ggaatatttg ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa
    38761 aagctaagcg atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta
    38821 attggcctag ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat
    38881 gaaccggata tcaagggaag cgggattcca caagtagtgg gacagcttcg cggggaatta
    38941 tcaatgaact ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt
    39001 gcgggccttt ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa
    39061 ggagctggac aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt
    39121 gcaagtagcg gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta
    39181 gaagaaatcc atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc
    39241 actgctaact tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac
    39301 cttccttctt tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc
    39361 atcttaggtt ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg
    39421 cctttaccag cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat
    39481 ttatggccta gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc
    39541 ttgccaatca ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct
    39601 tacggtgggg gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc
    39661 ggtagctttt atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat
    39721 tttgtcgtta ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca
    39781 attattttag tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct
    39841 gctcttgttg cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta
    39901 ttggaacgtc tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt
    39961 gattttccag taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg
    40021 cctaaaaata tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt
    40081 gatacgatca tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca
    40141 agaatcaagc aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa
    40201 cgaacatgga aaaactattt tacaacaact attcatctac actacttgat tctatatgat
    40261 tctataaaaa aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct
    40321 gctactgcag gagcttttct caataaacca taaatcacac cagatacgac tgctccgata
    40381 gcaataaaca ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg
    40441 tgaggagcca gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca
    40501 ataataaaac ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc
    40561 gtgacaaacg atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg
    40621 aatttattct taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca
    40681 gccataacag aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct
    40741 gtaccaaaca cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca
    40801 gctaataatg caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc
    40861 aatgcatcat tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc
    40921 agtaagccaa acactggata aaagaggatc gttttaattc catccaatga ttttggtaag
    40981 ttcttgaata atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt
    41041 gcacctaaga atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc
    41101 aaaccagggc ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa
    41161 ccaaatgcag cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga
    41221 tttgataatt gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata
    41281 gcgattcctc caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg
    41341 taaattcttt gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca
    41401 tcaccatgga atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta
    41461 cggataccat cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct
    41521 acttttttgt ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat
    41581 cggtttttga ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc
    41641 gcttttttct tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct
    41701 gtaacggcaa caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct
    41761 tgttctgctt tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact
    41821 tcttctggtg tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga
    41881 gatagtgctg ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg
    41941 aaaaacagat aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt
    42001 gcgaataaaa ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca
    42061 tcaccaagac ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg
    42121 atatcggaaa tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt
    42181 ttatcagtcg cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc
    42241 atgattatcc tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat
    42301 caatcaattc gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc
    42361 cccatttaaa ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa
    42421 tcattgaatc ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat
    42481 aagttccttg ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct
    42541 gtgccccttc agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg
    42601 ttgtgttgta aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa
    42661 caagtgcatt ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc
    42721 gaatgatatc aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg
    42781 acaatacgac gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt
    42841 cttcatctga aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt
    42901 tgatattgat tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt
    42961 caagccactt cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt
    43021 gagaatcgat ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga
    43081 agtcttcctt cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat
    43141 caattgatgg attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt
    43201 ttcacggaat tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc
    43261 caaaggtgca acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa
    43321 tgtctcttcg gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt
    43381 cgtgaatcct tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa
    43441 attttttagt tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt
    43501 tccacctaaa ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga
    43561 atttgtcact actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga
    43621 gccagcgtct aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat
    43681 ttgctgtttt tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa
    43741 taattgctct ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc
    43801 ttgtaaatca cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga
    43861 tttcacgatt ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag
    43921 cattttcacg atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa
    43981 tcagtcaaaa acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag
    44041 aaagactggt tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg
    44101 cttcttgttt ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa
    44161 taaagtccca tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta
    44221 ggaacaaaaa agagacagac ggataataat gatgccggaa ccattattcg ataatagagt
    44281 tgtttatcca ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg
    44341 gtaatctaaa aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact
    44401 tcgccgcttt ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt
    44461 taacggtaat tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc
    44521 ttgatatttt tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc
    44581 ttcgattcca ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga
    44641 tcgattctcg tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct
    44701 ttctttataa tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt
    44761 tttaggaaca ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt
    44821 gtaaatgtat tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg
    44881 atcaaaccca agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca
    44941 gttaaagggg tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt
    45001 ttctacttgt tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact
    45061 ataatacatt tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg
    45121 aaattggttg caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc
    45181 gatttcatcc tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat
    45241 aaactcgcta taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat
    45301 gaagtctcga atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat
    45361 caataattga ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa
    45421 ccaactttcg tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata
    45481 cggatccgct tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa
    45541 aatagcccaa tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat
    45601 agtcatcaat aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat
    45661 ttaattataa ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac
    45721 gattacatga tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt
    45781 gaacgtacag ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca
    45841 gttgcaccaa aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa
    45901 aaagacaaga aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg
    45961 aaagagtttg actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg
    46021 ctgcccacgc ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat
    46081 tgcgatcaaa atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag
    46141 ggatcttatc tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt
    46201 gatacatctg gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg
    46261 cacgtagctg gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct
    46321 cagttttgga tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct
    46381 cgatcaaata aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat
    46441 tctttcgatc aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca
    46501 gagctatttc tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa
    46561 tttcagcttg atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg
    46621 gctgtcgcaa aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc
    46681 tctgaatctt tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata
    46741 atcacttttt cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct
    46801 tcaatgatgg aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg
    46861 gctctcaacg cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga
    46921 tattgtttga ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc
    46981 aatttctcta aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat
    47041 tttagtaaat cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg
    47101 ttttgtgtac caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg
    47161 ccatcaaatg caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct
    47221 tgatgtggat gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt
    47281 ttcagcaagg cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg
    47341 cttttacgcg caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc
    47401 acattgtaat cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca
    47461 taaccagctt ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt
    47521 gctaatgttt gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg
    47581 cttttttgca gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag
    47641 tgattgtata cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta
    47701 ttcaatactt caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct
    47761 tcataaaatt cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag
    47821 acccctggac cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag
    47881 cgtacggtct cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa
    47941 agtatgcaag aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa
    48001 aaagaaaaag tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa
    48061 caaaaaataa ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt
    48121 tctagaagta gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc
    48181 gctgtacctg tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct
    48241 tctgtcaaca cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg
    48301 atttctgcat tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg
    48361 atcacaagat ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct
    48421 tcttctgtag aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg
    48481 ttgttcaaga attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat
    48541 tcaacgattt ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc
    48601 gctgcttttt cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat
    48661 gttgatactt cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt
    48721 gcttttactt catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct
    48781 tgataatctt ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact
    48841 aattgatctt caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg
    48901 ccttttccgc catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta
    48961 tcgccatttt cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca
    49021 cgtttgatac gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact
    49081 agatctttat attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc
    49141 cagtcttgac ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg
    49201 ccagcttctt tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca
    49261 tataaagctt cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg
    49321 aatccaggta cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg
    49381 atttcgtctt gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat
    49441 gttgcagtca tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc
    49501 atgcatacct ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga
    49561 atccttgttt ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc
    49621 atttgctgca aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct
    49681 tcatacatcg tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct
    49741 tcgatgtatg tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt
    49801 aacacggaac taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc
    49861 tggtaacttg cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt
    49921 tcttcaatcg tcaaatagaa aacttgatgg cattcgttca attgcctaag tgtctccagt
    49981 aattttgccc ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg
    50041 ataaactcat gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt
    50101 tccaacgaag gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt
    50161 tgtccggata attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca
    50221 taaaaatctg tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac
    50281 aagtcaaaat aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt
    50341 gcttgcttca atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta
    50401 ttagcctcaa agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga
    50461 tattgctggt ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga
    50521 aaatcaatcg gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt
    50581 agcaaaacaa aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt
    50641 ttagatctga cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg
    50701 ttgacttcct ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa
    50761 aaatcgccca gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc
    50821 aaaggaaatt ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta
    50881 agttcttctt catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg
    50941 ccatgattga atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat
    51001 ccgtagagac tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca
    51061 agtgttaaac gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg
    51121 ataaaaaaac ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt
    51181 gtgtccgtat actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc
    51241 tctctttcaa aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc
    51301 tgctgttgct tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact
    51361 cacctccgta ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg
    51421 ataagatctg actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta
    51481 accatgtact agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa
    51541 gctgttgttc ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta
    51601 aacctacttc ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt
    51661 cttcttcaga tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt
    51721 cgatatatgc ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca
    51781 tatctttcaa cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc
    51841 ctgaataatt caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg
    51901 caaagatttt atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca
    51961 tgctgacata cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt
    52021 ccgcactttc gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg
    52081 ttggatcttc ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag
    52141 aagcaaataa acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga
    52201 ttttttgcgt actgtctccc tcttccaaca catttggagc tagttcttca tccccatata
    52261 accaactgtt caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa
    52321 ctaacgaaac cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca
    52381 ttctttctcc tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag
    52441 tgtcgaaccg ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt
    52501 catcttctac cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt
    52561 ggatgctatc ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg
    52621 cttcgaattt ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat
    52681 ccatttttac tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg
    52741 atagataaaa acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata
    52801 taccaattta ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt
    52861 taaatgtttc cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag
    52921 gtctgtgtaa acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg
    52981 gactgcactc tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc
    53041 ctgcagctgg aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg
    53101 aattgttgga atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa
    53161 attagaatac cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta
    53221 tctggtcaaa gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac
    53281 aggaaatata ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa
    53341 cgcccatcca catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga
    53401 aaactttgaa gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga
    53461 tactgagatt atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc
    53521 gtttttaaaa gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac
    53581 agcacctgat gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga
    53641 tggattcaat gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt
    53701 tgagattgaa gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt
    53761 tgcaggtgac atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga
    53821 gaaaggtacc tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg
    53881 tcgtatcgct caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt
    53941 cgatacattg tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc
    54001 ggggttagca ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt
    54061 agccagtgaa tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt
    54121 gagtcaaagt ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg
    54181 ctacccttca cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt
    54241 tacactgcta ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc
    54301 acaaatagct gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac
    54361 ttctcttgta ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt
    54421 catcgcccaa aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa
    54481 tgccttttac attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt
    54541 gaaagaaatc agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac
    54601 gattgctttg atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg
    54661 tgcgcatact cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat
    54721 tgtctcagag ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta
    54781 tctaactact ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt
    54841 acggggattc gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata
    54901 aaagaacaag gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga
    54961 aaatatctct taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata
    55021 gtaattaact ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc
    55081 ggaaggacga actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac
    55141 ttttttgatt gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa
    55201 tttttgcgga tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt
    55261 catgatattc agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa
    55321 gaaaatcatg tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc
    55381 tacaacgtaa cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc
    55441 tttgtggaat cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt
    55501 tttttctgcc aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc
    55561 aagttcatcc accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc
    55621 tcctttttca acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt
    55681 gatatttaac ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa
    55741 caaacgatta acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc
    55801 ggaaaggtca ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg
    55861 aaattcttct acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc
    55921 atctaataat gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc
    55981 gaaaaatttg attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc
    56041 gcttgctttt tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg
    56101 gaacacttcg atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc
    56161 tgagattctt gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg
    56221 gcttaggaca taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt
    56281 gttggcttca ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact
    56341 cctttaactt aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct
    56401 gatgtttctt ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg
    56461 gttgttgtcg atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta
    56521 ttttcttgtg tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt
    56581 gtaatctcca cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca
    56641 attttacgag ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca
    56701 ccgatcgatg caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg
    56761 aaatcatagc tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc
    56821 gacttgcttg gcttgatcga atcagctctc actgttactt gcactggatc agagacgata
    56881 cttaatactt cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca
    56941 tctgtgacca tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg
    57001 tcacctgtag tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca
    57061 gatggtgtga acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc
    57121 tcaaccgtta ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt
    57181 tgaattttta gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg
    57241 cggaacatcc gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa
    57301 agtttgacag tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa
    57361 atcttgatcg gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt
    57421 ggaacggact ggccatttgc attgaagtac agcgtaatac tgaacagcaa tgcaataaaa
    57481 ctgtaaagaa tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca
    57541 taccgtctag gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta
    57601 gttctctacg caagattgcc aaatattctt gctgggataa gccagccatc agttcattat
    57661 ctaacgtgat gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga
    57721 cttcgcttat accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac
    57781 tttctgatag aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg
    57841 caccgtcatg cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat
    57901 ctgcatctaa agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag
    57961 tgatcaacgc accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga
    58021 tcatcttctc atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa
    58081 gatgttccag tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt
    58141 acgtaatgac ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga
    58201 ttcggataac aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca
    58261 gcagttggat cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca
    58321 aaaaattcag agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa
    58381 gttccccgat ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt
    58441 atatcatatg ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat
    58501 aattttcaag aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac
    58561 ttcagagaat tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg
    58621 ttccattttt ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc
    58681 ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg
    58741 ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg
    58801 tgttttagcg tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt
    58861 atctagcgag gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg
    58921 ttttttgtag tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg
    58981 atataatgac caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat
    59041 tccgccaagc aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg
    59101 atacttttct gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat
    59161 ttgcgccaca taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc
    59221 atactttcca ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg
    59281 accgccagta ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct
    59341 ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat
    59401 catccattgg ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg
    59461 gaaaattgat cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc
    59521 ttcactctca aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac
    59581 ttttggcaat acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag
    59641 aagcatacca taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc
    59701 ttgaccacat tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga
    59761 acagcctgtc gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac
    59821 ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa
    59881 ttgtgtagac aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat
    59941 tgcaaaagcc caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc
    60001 tttttctttt gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga
    60061 aacttggata cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc
    60121 tggcgcttct tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca
    60181 aacaaaggca cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa
    60241 agcgacgccc cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc
    60301 atttgctaca agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag
    60361 tatttcttgt atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct
    60421 ccaatggtct gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat
    60481 gtttttttct acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag
    60541 cagcggaagc acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat
    60601 tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt
    60661 atagaatcta atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc
    60721 taattgttcg tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt
    60781 gaccaagtca tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc
    60841 gacaagatac gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc
    60901 gccagatttt ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc
    60961 aataatcttg atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa
    61021 cttggcctgg aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg
    61081 ataagtcaca tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt
    61141 tttcaattca tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc
    61201 gtagcgacca ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc
    61261 taataagaga ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt
    61321 ttttactgta tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg
    61381 gatattcaaa aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat
    61441 cgtctgtgca actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata
    61501 agtcaccaag tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac
    61561 gatttcaggt aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc
    61621 tggattctgg ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa
    61681 tgaagctaat ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat
    61741 tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc
    61801 ttcaatagaa gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc
    61861 agctaacata gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat
    61921 attcaacgca tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc
    61981 cccagcaatc ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt
    62041 agttgtcagt gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga
    62101 ttgcatttct actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca
    62161 ttgatctact aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc
    62221 ggtaaatgca taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg
    62281 catgattcat catccttgtt catattctct cttattatct gttccattat aacgtaaaac
    62341 atgccatttt tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat
    62401 ctaattttta gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat
    62461 aagcgaaaga ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca
    62521 caaaaaggaa tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac
    62581 ttagcttgtc cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca
    62641 gtccaaagcg ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta
    62701 cagcgaggga acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa
    62761 gagatcaaac aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta
    62821 gcggattttg cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa
    62881 gctgtcatca acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt
    62941 aaagaaatct ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa
    63001 taaaaaaata aggagcgtg
//
LOCUS       DAFGZE010000006        61077 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.006, whole genome shotgun sequence.
ACCESSION   DAFGZE010000006 DAFGZE010000000
VERSION     DAFGZE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.006"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            130..858
                     /locus_tag="LTU54_000394"
     CDS             130..858
                     /locus_tag="LTU54_000394"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBL8295134.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            858..1112
                     /gene="purS"
                     /locus_tag="LTU54_000395"
     CDS             858..1112
                     /gene="purS"
                     /locus_tag="LTU54_000395"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HBL8295135.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1114..1785
                     /gene="purQ"
                     /locus_tag="LTU54_000396"
     CDS             1114..1785
                     /gene="purQ"
                     /locus_tag="LTU54_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HBL8295136.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1786..4008
                     /gene="purL"
                     /locus_tag="LTU54_000397"
     CDS             1786..4008
                     /gene="purL"
                     /locus_tag="LTU54_000397"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HBL8295137.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYLPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3993..5432
                     /locus_tag="LTU54_000398"
     CDS             3993..5432
                     /locus_tag="LTU54_000398"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBL8295138.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5455..6507
                     /locus_tag="LTU54_000399"
     CDS             5455..6507
                     /locus_tag="LTU54_000399"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBL8295139.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6504..7082
                     /gene="purN"
                     /locus_tag="LTU54_000400"
     CDS             6504..7082
                     /gene="purN"
                     /locus_tag="LTU54_000400"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBL8295140.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7079..8614
                     /gene="purH"
                     /locus_tag="LTU54_000401"
     CDS             7079..8614
                     /gene="purH"
                     /locus_tag="LTU54_000401"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBL8295141.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALVFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8630..9904
                     /gene="purD"
                     /locus_tag="LTU54_000402"
     CDS             8630..9904
                     /gene="purD"
                     /locus_tag="LTU54_000402"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBL8295142.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10026..10940
                     /locus_tag="LTU54_000403"
     CDS             10026..10940
                     /locus_tag="LTU54_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL8295143.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11073..12638)
                     /locus_tag="LTU54_000404"
     CDS             complement(11073..12638)
                     /locus_tag="LTU54_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL8295144.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12631..14082)
                     /locus_tag="LTU54_000405"
     CDS             complement(12631..14082)
                     /locus_tag="LTU54_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL8295145.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIRESKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14096..15112)
                     /locus_tag="LTU54_000406"
     CDS             complement(14096..15112)
                     /locus_tag="LTU54_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8295146.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15332..16420
                     /locus_tag="LTU54_000407"
     CDS             15332..16420
                     /locus_tag="LTU54_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HBL8295147.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16474..18000
                     /locus_tag="LTU54_000408"
     CDS             16474..18000
                     /locus_tag="LTU54_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBL8295148.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18002..19207
                     /locus_tag="LTU54_000409"
     CDS             18002..19207
                     /locus_tag="LTU54_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295149.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19464..20441
                     /gene="guaC"
                     /locus_tag="LTU54_000410"
     CDS             19464..20441
                     /gene="guaC"
                     /locus_tag="LTU54_000410"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBL8295150.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            20705..22129
                     /locus_tag="LTU54_000411"
     CDS             20705..22129
                     /locus_tag="LTU54_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL8295151.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            22590..23699
                     /gene="pdhA"
                     /locus_tag="LTU54_000412"
     CDS             22590..23699
                     /gene="pdhA"
                     /locus_tag="LTU54_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HBL8295152.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            23702..24679
                     /locus_tag="LTU54_000413"
     CDS             23702..24679
                     /locus_tag="LTU54_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBL8295153.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            24704..26347
                     /locus_tag="LTU54_000414"
     CDS             24704..26347
                     /locus_tag="LTU54_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBL8295154.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVIKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26355..27761
                     /gene="lpdA"
                     /locus_tag="LTU54_000415"
     CDS             26355..27761
                     /gene="lpdA"
                     /locus_tag="LTU54_000415"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBL8295155.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            27923..28198
                     /locus_tag="LTU54_000416"
     CDS             27923..28198
                     /locus_tag="LTU54_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HBL8295156.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28204..28971
                     /locus_tag="LTU54_000417"
     CDS             28204..28971
                     /locus_tag="LTU54_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HBL8295157.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29139..30968
                     /gene="typA"
                     /locus_tag="LTU54_000418"
     CDS             29139..30968
                     /gene="typA"
                     /locus_tag="LTU54_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBL8295158.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31229..33001
                     /gene="recQ"
                     /locus_tag="LTU54_000419"
     CDS             31229..33001
                     /gene="recQ"
                     /locus_tag="LTU54_000419"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBL8295159.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33115..33417
                     /locus_tag="LTU54_000420"
     CDS             33115..33417
                     /locus_tag="LTU54_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HBL8295160.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            33420..34583
                     /locus_tag="LTU54_000421"
     CDS             33420..34583
                     /locus_tag="LTU54_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL8295161.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            34609..38037
                     /locus_tag="LTU54_000422"
     CDS             34609..38037
                     /locus_tag="LTU54_000422"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBL8295162.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38058..39182
                     /locus_tag="LTU54_000423"
     CDS             38058..39182
                     /locus_tag="LTU54_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295163.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39172..39609
                     /locus_tag="LTU54_000424"
     CDS             39172..39609
                     /locus_tag="LTU54_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL8295164.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            39593..39931
                     /locus_tag="LTU54_000425"
     CDS             39593..39931
                     /locus_tag="LTU54_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HBL8295165.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40050..40610
                     /gene="rsmD"
                     /locus_tag="LTU54_000426"
     CDS             40050..40610
                     /gene="rsmD"
                     /locus_tag="LTU54_000426"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBL8295166.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            40607..41098
                     /gene="coaD"
                     /locus_tag="LTU54_000427"
     CDS             40607..41098
                     /gene="coaD"
                     /locus_tag="LTU54_000427"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBL8295167.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41088..42131
                     /locus_tag="LTU54_000428"
     CDS             41088..42131
                     /locus_tag="LTU54_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HBL8295168.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42290..43018
                     /locus_tag="LTU54_000429"
     CDS             42290..43018
                     /locus_tag="LTU54_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBL8295169.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43134..43379
                     /locus_tag="LTU54_000430"
     CDS             43134..43379
                     /locus_tag="LTU54_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL8295170.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            43376..43594
                     /locus_tag="LTU54_000431"
     CDS             43376..43594
                     /locus_tag="LTU54_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBL8295171.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            43730..44269
                     /locus_tag="LTU54_000432"
     CDS             43730..44269
                     /locus_tag="LTU54_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HBL8295172.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44262..46535
                     /locus_tag="LTU54_000433"
     CDS             44262..46535
                     /locus_tag="LTU54_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HBL8295173.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            46573..47619
                     /gene="holA"
                     /locus_tag="LTU54_000434"
     CDS             46573..47619
                     /gene="holA"
                     /locus_tag="LTU54_000434"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBL8295174.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(47708..47962)
                     /locus_tag="LTU54_000435"
     CDS             complement(47708..47962)
                     /locus_tag="LTU54_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBL8295175.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48362..48631)
                     /locus_tag="LTU54_000436"
     CDS             complement(48362..48631)
                     /locus_tag="LTU54_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295176.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49101..49556)
                     /locus_tag="LTU54_000437"
     CDS             complement(49101..49556)
                     /locus_tag="LTU54_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBL8295177.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(49694..51622)
                     /locus_tag="LTU54_000438"
     CDS             complement(49694..51622)
                     /locus_tag="LTU54_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL8295178.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(51819..52652)
                     /locus_tag="LTU54_000439"
     CDS             complement(51819..52652)
                     /locus_tag="LTU54_000439"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBL8295179.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(52844..53548)
                     /locus_tag="LTU54_000440"
     CDS             complement(52844..53548)
                     /locus_tag="LTU54_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL8295180.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(53571..54197)
                     /locus_tag="LTU54_000441"
     CDS             complement(53571..54197)
                     /locus_tag="LTU54_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL8295181.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            54523..56898
                     /locus_tag="LTU54_000442"
     CDS             54523..56898
                     /locus_tag="LTU54_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBL8295182.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(56922..57413)
                     /locus_tag="LTU54_000443"
     CDS             complement(56922..57413)
                     /locus_tag="LTU54_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HBL8295183.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            57600..58520
                     /locus_tag="LTU54_000444"
     CDS             57600..58520
                     /locus_tag="LTU54_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL8295184.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            58674..59018
                     /locus_tag="LTU54_000445"
     CDS             58674..59018
                     /locus_tag="LTU54_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HBL8295185.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59208..59708)
                     /locus_tag="LTU54_000446"
     CDS             complement(59208..59708)
                     /locus_tag="LTU54_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295186.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60236..60319
                     /locus_tag="LTU54_000447"
     tRNA            60236..60319
                     /locus_tag="LTU54_000447"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60270..60272,aa:Leu,seq:cag)
     gene            complement(60643..>61077)
                     /locus_tag="LTU54_000448"
     CDS             complement(60643..>61077)
                     /locus_tag="LTU54_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HBL8295187.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20045 a        10551 c        13106 g        17375 t
ORIGIN      
        1 aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa
       61 atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga
      121 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
      181 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      241 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      301 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      361 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
      421 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
      481 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
      541 tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct
      601 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
      661 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
      721 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
      781 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
      841 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
      901 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
      961 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
     1021 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
     1081 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1141 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1201 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1261 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1321 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1381 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     1441 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     1501 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     1561 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     1621 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     1681 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     1741 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     1801 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     1861 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     1921 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     1981 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     2041 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     2101 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2161 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2221 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2281 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2341 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     2401 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     2461 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     2521 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     2581 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     2641 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     2701 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     2761 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     2821 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     2881 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     2941 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatctgcca
     3001 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     3061 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3121 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3181 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3241 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3301 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3361 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     3421 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     3481 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     3541 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     3601 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     3661 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     3721 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     3781 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     3841 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     3901 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     3961 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     4021 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     4081 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4141 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4201 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4261 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4321 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4381 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     4441 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     4501 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     4561 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     4621 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     4681 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     4741 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     4801 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     4861 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     4921 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     4981 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     5041 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     5101 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5161 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5221 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5281 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5341 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     5401 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     5461 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     5521 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     5581 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     5641 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     5701 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     5761 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     5821 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     5881 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     5941 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     6001 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     6061 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6121 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6181 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6241 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6301 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6361 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     6421 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     6481 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     6541 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     6601 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     6661 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     6721 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     6781 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     6841 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     6901 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     6961 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     7021 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     7081 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7141 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7201 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7261 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7321 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7381 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     7441 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     7501 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     7561 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     7621 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     7681 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     7741 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     7801 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     7861 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     7921 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     7981 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     8041 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     8101 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8161 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8221 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8281 attagtgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8341 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     8401 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     8461 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     8521 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     8581 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     8641 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     8701 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     8761 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     8821 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     8881 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     8941 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     9001 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     9061 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9121 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9181 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9241 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9301 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9361 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
     9421 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
     9481 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
     9541 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
     9601 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
     9661 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
     9721 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
     9781 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
     9841 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
     9901 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
     9961 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
    10021 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
    10081 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10141 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10201 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10261 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10321 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10381 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    10441 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    10501 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    10561 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    10621 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    10681 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    10741 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    10801 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    10861 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    10921 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    10981 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    11041 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    11101 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11161 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11221 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11281 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11341 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    11401 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    11461 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    11521 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    11581 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    11641 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    11701 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    11761 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    11821 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    11881 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    11941 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    12001 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    12061 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12121 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12181 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12241 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12301 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12361 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    12421 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    12481 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    12541 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    12601 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    12661 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    12721 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    12781 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    12841 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    12901 tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    12961 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    13021 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    13081 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13141 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13201 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13261 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13321 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13381 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    13441 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    13501 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    13561 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    13621 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    13681 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    13741 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    13801 gctctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    13861 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    13921 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    13981 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    14041 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    14101 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14161 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14221 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14281 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14341 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    14401 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    14461 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    14521 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    14581 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    14641 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    14701 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    14761 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    14821 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    14881 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    14941 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    15001 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    15061 tcatttggta agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15121 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15181 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15241 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15301 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15361 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    15421 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    15481 cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca
    15541 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    15601 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    15661 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    15721 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    15781 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    15841 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    15901 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    15961 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    16021 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    16081 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16141 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16201 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16261 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16321 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16381 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    16441 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    16501 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    16561 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    16621 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    16681 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    16741 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    16801 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    16861 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    16921 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    16981 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    17041 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    17101 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17161 atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct
    17221 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17281 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17341 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    17401 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    17461 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    17521 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    17581 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    17641 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    17701 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    17761 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    17821 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    17881 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    17941 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    18001 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    18061 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18121 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18181 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18241 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18301 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18361 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    18421 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    18481 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    18541 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    18601 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    18661 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    18721 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    18781 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    18841 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    18901 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    18961 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    19021 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    19081 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19141 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19201 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19261 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19321 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19381 attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat
    19441 gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc
    19501 ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac
    19561 atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg
    19621 cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc
    19681 gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg
    19741 tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt
    19801 atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata
    19861 ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg
    19921 tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag
    19981 tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta
    20041 gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg
    20101 gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg
    20161 ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat
    20221 actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa
    20281 tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc
    20341 aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg
    20401 tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag
    20461 gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct
    20521 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
    20581 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
    20641 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
    20701 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
    20761 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
    20821 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
    20881 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
    20941 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
    21001 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
    21061 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
    21121 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
    21181 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
    21241 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
    21301 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
    21361 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
    21421 aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt
    21481 tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg
    21541 tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa
    21601 ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc
    21661 gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt
    21721 tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca
    21781 aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa
    21841 aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa
    21901 gcttcattca attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga
    21961 ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt
    22021 tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc
    22081 gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt
    22141 aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg
    22201 aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat
    22261 gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa
    22321 cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt
    22381 ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca
    22441 tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga
    22501 agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt
    22561 cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc
    22621 aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg
    22681 gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa
    22741 tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag
    22801 gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt
    22861 acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa
    22921 aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt
    22981 cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac
    23041 aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt
    23101 atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg
    23161 cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc
    23221 gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg
    23281 gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg
    23341 aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac
    23401 cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt
    23461 ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt
    23521 ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg
    23581 ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag
    23641 tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat
    23701 catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa
    23761 tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc
    23821 aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga
    23881 atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga
    23941 aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg
    24001 tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg
    24061 tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc
    24121 tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc
    24181 ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt
    24241 ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg
    24301 tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc
    24361 agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc
    24421 gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt
    24481 ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg
    24541 cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc
    24601 atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa
    24661 agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa
    24721 attgccggat atcggtgaag gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc
    24781 aggagatacg atcaatgaag atgatacatt actagaagtt caaaatgaca aatcagtaga
    24841 agaaatcccg tcacctgtta caggaacggt taaaaatgtg attgttccag aaggtaccgt
    24901 agcaaatgtt ggggatgtat tagtagaaat cgatgcacca ggacacgaag acaatgaagg
    24961 ggacagtggt gtagccgcag aatcccaaac tccagctaaa ccggctgctg aacccactgt
    25021 tgatacagaa tcagctggat cgtcaagcga aggagtcttc caatttaagt taccggatat
    25081 cggtgaagga atcgcagaag gtgaaatcgt caaatggttc gtcaaaccag gagatacgat
    25141 caatgaagat gatacattac tagaagttca aaatgacaaa tcagtagaag aaattccgtc
    25201 acctgtcaca gggacagtta aaaacgtgat tgttccagaa ggcactgtag caaatgttgg
    25261 ggatgtatta gtagaaatcg atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc
    25321 atcagctgaa gcaccaaaag aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc
    25381 tgccgatcca aataaacgtg tattggctat gccatctgtt cgtcaatttg cccgtgaaaa
    25441 agatgtagat atctcacaag taactgcaac tggaaaaggt ggcagagtaa taaaagagga
    25501 catcgaaaac ttcttggcag gtggtccaag cagtgcacca gcgaaatcag aagcgccaga
    25561 agcggcagca ccgaaagaag cagctcctgc agcagaaagc aaaccagctg caccagcaaa
    25621 accattcaag tccaacttag gagacttgga agaacgtgta gcaatgacgc caacacgtaa
    25681 agctatcgct aaagcaatgg tcaacagtaa gcatacagct ccacacgtga cattgcatga
    25741 tgaagtagaa gtgtctaaac tatgggataa tcggaaacga tttaaagaag ttgctgctgc
    25801 aaatggtacg aaacttactt tcttgccata tgtcgtgaaa gctttaacag cgactgttaa
    25861 gaaataccca gtattgaatg catctatcga cgatgcaaac caagaaatcg tttataaaca
    25921 ttactacaat atcggtattg caactgatac ggatcacgga ctatatgtac caaatgtgaa
    25981 agatgcagac cgcaaaggaa tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt
    26041 agctcatgac ggcaaactat ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa
    26101 tatcggatct gtaggtggcg gttggtttac accagtcatc aactatccag aagtagctat
    26161 tttaggtgta ggaacgattg cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt
    26221 cggtcgtgtg atgaaactgt cattaagttt cgatcaccgt atcgtagatg gagcaactgc
    26281 gcaacaagca atgaataata tcaaacgtct tttagctgat ccagagctat taatgatgga
    26341 aggatgataa acaaatggta gttggagatt ttgctgtaga attagataca gttgtaattg
    26401 gtgcgggacc tggtggctat gtagctgcca tccgcgctgc tgaaatggga caaaaagtag
    26461 caatcattga aagagagtat attggcggtg tttgtttgaa tgtcggctgt attccttcaa
    26521 aagcactgat agctgcagga caccattatc aagagtcact agattctgaa atgtttggag
    26581 taactagtga aaacgtcaaa ctagattttg caaaaacaca agaatggaaa gaaaacaaag
    26641 ttgtccatac gttgacttct ggtgttggtt tcctattaaa aaaacataag gttgaaacaa
    26701 tcgaaggaga agcgttcttc gtagatgatc acacactacg cgtcattcat ccagattcag
    26761 ctcaaactta ttcgttcaat aacgcaatca tcgcaacagg ttcccgtcca atcgagattc
    26821 caggatttaa attcggagga cgtgtgttag attctactgg cggattagca ttgaaagaag
    26881 tacctaagaa atttgtgatc atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt
    26941 acgcaaactt aggagcagaa gtaacgattc ttgaaggctc accacaaatt ttaccaactt
    27001 acgaaaaaga tctagtaaaa ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg
    27061 taacgaatgc aatggcgaaa gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg
    27121 cagtggacgg gaaagaagaa tccgttactg ctgattatgt gatggtcact gtaggtcgtc
    27181 gtccgaatac agatgatatg ggcttagaac aagcgggtgt cgaagtagga gaacgaggat
    27241 tgatcacggt ggataaacaa ggacgtacga acgtacctaa catctatgcc atcggtgata
    27301 tcgttccagg ggcggcatta gcacacaaag ccagttatga agcaaaaatc gctgctgaag
    27361 caatctctgg caaaaaagta gctgtagatt ataaagcaat gccggcagta gccttcactg
    27421 atcctgaatt ggcttctgta ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag
    27481 caaaagcata taaattccca ttttcaggga acggtcgggc attatcgctt ggtaaaacag
    27541 aaggattcat ccgtctagtt acgacgatcg aagataacgt gcttattggc gcgcaaatcg
    27601 gtggagtcgg cgcaagtgat atggtctctg aattagcttt agcaatcgaa tcaggaatga
    27661 atgctgaaga tatcgccttg acgattcatc ctcatccatc tcttggcgaa atcgtcatgg
    27721 atgcttcgga attagcatta ggtttaccaa tccatatttg atttgaataa tacaggcgaa
    27781 acgcaactga aaaatcagtt gcgttttgtt ttttagaatt atcaataata ttattatgta
    27841 aacttaaaaa tatgacaact tcttggttta ttaccacttc ctatgctaca ctgaagagga
    27901 atatgaaata ggagagttga agatgcaaga atatcagtat ccacttgatt tagattggac
    27961 aacagacgaa atggtcattg taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa
    28021 agggataaac aatgaagctt ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc
    28081 cattggtgag gaaaaacgat tgggacggga atttgaaaaa gcttccggtt attctttata
    28141 tcgtacagtg cagcaagcaa aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga
    28201 taaatggaac agatgattga tcatatcaag tcctggcttg aagaagctgg tgagaaaatc
    28261 aaatcttctc aacaaacatt aacaatctcg caaaaatcaa atcgaacgga tctagtaaca
    28321 aatgtcgatc gaaacatcca gaattttcta atcgaaaaaa tccattcctt tgatccagag
    28381 gcgaaaatat tggcagaaga agaagggtgt aatcaattat cggatctcga tggaagagtc
    28441 tttatcatcg atcctattga tggcaccttg aattttgtga ttgaagggga aaatttttgt
    28501 attatgctcg ctgtttatga atccggcgtt ggaaagcttg gtttcattta tgacgtgatg
    28561 agaaatgaac ttttttgggg tggaaaagga ataggcgtgt ataaaaatga tgagccattg
    28621 cctagaccag caaataaatc cttatcggaa ggcttacttg gtgtgaatag ttatcttttc
    28681 ggtcataata aatatggaat ccgagaaatt gcggaaagca gcatggggac acgtatgtac
    28741 ggatgcgctg gtcttgaact agtggcaatc ttgaaaggaa accacattgc ttatatttca
    28801 aaccttagcc cttgggatta tgcagcggga aatgtcttat tggaagagtt tggtatggta
    28861 acaagcggtc tttttggtac tcctttgaca tttgaaaaaa gggaatacta tttagcaggg
    28921 acttcttgtg catatgcaga aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt
    28981 agaaatcatt cacaaaaatg ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa
    29041 aacattttac tatatttcat gaaaaaagtc tgctataatt agcagtcgag taaaatttgc
    29101 cctgtcataa agaaccaata aaaaaggagt tagttacatt gaattataga aacgatatcc
    29161 gtaacgtcgc aattattgcc cacgttgacc acggaaaaac aacattagtt gatgaactat
    29221 taaaacaatc agatacatta gacgcacata cgcaattgca agaacgtgcg atggactcaa
    29281 atgcgctaga aaaagaacgt ggtatcacga ttttagccaa aaacacagct gtagactata
    29341 aaggtattcg cgtaaacatc atggatactc caggacacgc ggacttcggt ggcgaagtag
    29401 aacggatcat gaaaatggtt gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa
    29461 tgcctcaaac acgttttgta ttgaaaaaag cactggaaca acatatcaca ccaatcgttg
    29521 tcgtaaacaa gattgacaaa ccttctgctc gtcctgagca tgtcgtagat gaagttcttg
    29581 aactattcat cgaattagga gcagatgatg atcagttaga ctttcctgtg atttatgcat
    29641 ctgcactaaa tggtacgtca agcttgtcag atgatccagc agatcaagaa ccaaccatgg
    29701 cacctatttt tgatacaatt attgaaaaaa tccctgctcc tgtcgataac agcgacgaac
    29761 cattacaatt ccaagtttca ttacttgact acaacgatta tgtaggtcgt atcggaatcg
    29821 gacgtgtatt tagaggaaca atcaaagtag gagaccaagt agcattaatt aaattagacg
    29881 gaacagttaa gaaattccgt gtaacgaaat tatttggttt ctttggacta aaacgtctag
    29941 agattcaaga agcaaaagct ggagacttga ttgctgtctc aggtatggaa gatatctttg
    30001 tcggtgaaac agttactcct gtggatcatc aagatgcgtt acctatcctt cacattgacg
    30061 aaccaacatt gcaaatgact ttcttagtta ataattcacc atttgccggt cgagaaggta
    30121 aattcgtaac cgctcgtaag attgaagaac gtttgatggc agaacttcaa acagatgtat
    30181 ccttacgagt tgaaccaaca aactctccag atgcatggac agtttctggt cgtggagaat
    30241 tgcatttgtc tatcttgatt gaaaacatgc gccgtgaggg atacgaattg caagtatctc
    30301 gtccagaagt tattgaaaaa gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc
    30361 aaatcgatac accagaagaa tatatgggta gtgttatcga gtccttgagc ttacgtaaag
    30421 gtgaaatgca agatatggtc catacaggaa acggacaaat ccgtttgaca ttcttaacac
    30481 ctgcccgcgg tttgattggt tattcaactg aattcctttc aatgacacgt ggttacggaa
    30541 tcatgaacca tacttttgac caatatctgc caatgcttcc tggtcaaatt ggcggacgtc
    30601 accaaggggc gcttgtttcg atcgatacag gaaaagcaac gacttattct atcatgagta
    30661 tcgaagaacg cggtacagtc ttcgtagaac caggtacaga agtatatgaa ggaatgatta
    30721 tcggggaaaa cagccgtgat aatgatttga ctgtcaacat cacgaaagca aaacaaatga
    30781 caaacgtacg gtctgctact aaagaccaaa catctgtcat caaaaaacca aaacaattga
    30841 cgttggaaga atctttggaa ttcttgaatg atgatgaata ttgcgaagta acaccagaaa
    30901 gcattcgctt gagaaaacaa attttagaga aaaatgctcg tgaaaaagca tctaaaaaga
    30961 aaaaataatc tgtaaaagga tgaagagaga aaccagcttg ttataaaaaa tagctggttt
    31021 ctttttttgt ccaactcttt tctgtggaga agtcatcttt gtcaatggac ataaattcag
    31081 aaaatacgcc gaaagacgtt taaaagactt ttaaaaaact tatttctaga gagtttttgt
    31141 ttcataataa gataatgttt cacgaaagga gatcaacttg tccttcgaag aaaatcgaag
    31201 taaaatggca agagaaagga gcgggaagat gaacgactta ttaaaacaat actttggcta
    31261 tgatgagttt agacctggtc aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt
    31321 attaggaatc atgcctaccg gaagtgggaa atcaatctgt taccagcttc cagctttact
    31381 attagatggg cttactgtgg ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga
    31441 cgcggcgaat caattaggta tacccgccac ttttatcaat agctcgttag atggttacga
    31501 aacagctaga cgcttccaag aaatcgatag acagcagtac cgtttacttt atatcgctcc
    31561 tgaacgtttt atcatgccgg attttatcca agcaatgaag cgatggaacg tccgtatgat
    31621 cgctattgac gaggcacatt gtatctctca gtgggggcat gatttccgcc caagttattt
    31681 acagatggcg aaccagctag atcaactgcc aaatcgtcct gtaattgttg ctttgacagc
    31741 tacagcgact gtgcaagtag ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa
    31801 ccatattcag actggttttg aacgagaaaa tctacgattt caagtaatta aagatcaaaa
    31861 aaaagaacaa tatctaatcg aatatttaaa gatcaacaaa aatcaatctg gtatcattta
    31921 tgcagcaacg agaaaagaag tagaccgtct ctatcatttg cttaaaaaat tcgatttttc
    31981 cgtgggtaga tatcatggtg gtctaaatga gaatgagcga acagaaatgc aagaagcctt
    32041 tttatacgat cgcttgcagc tgattgtagc gacaaatgca tttggtatgg gtataaataa
    32101 aagcaatgtc cgttttgtta tccattatca aattcctggc tctctagaag catactatca
    32161 ggaagctggt cgggcgggaa gagatggtct ttccagtgaa gccatcctgc ttttctctcc
    32221 tcaagatatc caagtccaaa agttttttat ccagcagtcc caaagagaag aagggcaaaa
    32281 gcaaaaagaa tatgaaaaat taaaagcgat gacagaatac gtgtatattg agtcctgtct
    32341 tcagcaatac atcctaaact attttggcga gacaagcagt ccgtgcaacc gttgtggtaa
    32401 ctgtttagat gacagagaaa tagtagaagt aacgacagaa gcacagatgg ttctttcttg
    32461 cttaaaacgt atgggtgaga actatggtaa acaaatgctg atgaaagtct tagcaggatc
    32521 gaaagaacaa aagctgcaag ctttaggttt tggacattta tctacctatg gactgatgaa
    32581 aaaccaatcg caaaaagaaa cgatgcagtt aattgaatat ttgatatcga atggctatct
    32641 tctgaccatc aatggtgagt atcctgtttt gaaagtaaca gaaagaggga tacaagtatt
    32701 gaaaggacaa gaatctgttt atcgaaagga acccaagaaa gtccagcagc tatcggatga
    32761 agaaaccgat acactatttg aagtgctgcg ggaactgcga acggatctag catcagaagc
    32821 aggcgttccc ccgtatgtcg tattttcaga ttccacattg aaagaaatga gccgaatacg
    32881 tccatcaagc agattggaga tgcttcagat aaaaggagtt ggccaaagca agcttgataa
    32941 atatggcgaa gcctttttat ctcggataaa aaatgcggac tctaacgttc tggataagta
    33001 gaaagaacga atacgtaaaa atgaataaaa acaactggtt aaatctgttt tttttgctcc
    33061 ttatgaaaat caaatatgtt ataattaagg aattagaatg ggaggtaagc acagatggat
    33121 tctttgtcga aaaaacttgc gatccaagta ttaaatgatg aagcagaacg gatcaaacga
    33181 ttaatccgaa atcaaaacaa cagtctctgt atctcacaat gcaaagcctt cgaagaagtg
    33241 gtagatacac agatgtacgg cttttcccgg caagtcggtt ttgctgtccg tataggagta
    33301 atcggaaaga acgagggcca gctgatgtta agtgagctag aacgagaatt gaatcagtta
    33361 tatagtgagg tttaccaaga aatttatgat aaaaaagaag ttggcaagga ggaatagact
    33421 tgcctaagaa agtgaaaaaa aggcatctat tagactatag catcttgatt ccctatttaa
    33481 ttttatgtgt attgggattg atcatggtat atagttctac gtcgtatcta ttattagaaa
    33541 acggacaaaa tccgtccgca tcagtcatta accagagtat tttttgggtg cttagcttga
    33601 ttgctatcgc actactttat aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca
    33661 tggcagccat cgcagttttg acgattttgc tgctgattgt tgtgtttttt ggtaaggaga
    33721 tcaatggggc gaaaggttgg ctgcaaattg caggattctc gatccaacct gccgaatacc
    33781 taaaaatcat ttctatctgg tacctgtctc tgactttatc taaaagacaa aacagtgtac
    33841 aaaaagattt tcttgggact gtgaaacgtc cacttgcaat ggttatcgga cttactgcat
    33901 tagtcgctat tcttcccgat tttggtaatg cggcagttat ctttttgatt attttggttc
    33961 tgcttttagc cagcggagtc aattatgtgt atactttgat tgtaggagta ggaggctttt
    34021 gtctaagtac ttttacgatc tggctgatca atatcacaaa cggaaagatc ttacccggac
    34081 gtttacagta tatctataat cgatttgcta tttaccaaaa tcctttctca gacgaattaa
    34141 ataaagggca tcaactagtc aacggctact atgccatgtt caacggagga ctatttggca
    34201 ggggattagg taacagtatc cagaaaaaag gatttttaca agaagcacaa accgatttta
    34261 tttatgcaat cgtagtggaa gaactgggag tgattatggg gattttgata ttagcgttgc
    34321 tattctttat gattgtgcgc attgtccttg taggtatccg ttcaaaagat ccgttcaatt
    34381 cgcttttgtg cattgggatc ggcgcaatgt ttttgatcca agtatttgtc aatcttggag
    34441 gaattactgg cgttattcct ttgacaggga tcacgttccc atttttaagt caaggaggct
    34501 ccagtttgct gatgctttcg atttgtgtcg gctttgtcct caacatcagt gcggatgaaa
    34561 aacgcaagag cttaggttta taataaaaaa agatgtagga gtggttcgat gaaaaaagtt
    34621 ttagttgcta acaggggtga aatcgctgta cgtgtatttc gtgcttgtac agaattagga
    34681 attaaaactg ttggaatcta tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg
    34741 gatgaagcat atttagtagg acaaggaaaa aaaccgattg atgcgtatct agatattgaa
    34801 gggatcattt ctatcgcgaa agaatgcgga gctgacgcta ttcatccggg ttacggtctt
    34861 ttgtcggaaa acttgaattt tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga
    34921 cccaaactgc atcatttaga tatttttgga gataaaatca aagcaaaagc tgcagctatc
    34981 gaagcgggga ttgcttcgat tccaggcaca gatggaccaa tcgcttctat agacgatgcc
    35041 ttggagtttg cgaaacaata cggttatccg attatgatca aggcagctct aggcggcggc
    35101 ggacgcggca tgcgtgtagc tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg
    35161 aaaagcgaag caaaagcagc ctttggtagt gatgaagtgt atgttgaaaa atacatcgct
    35221 aatcctaagc atatcgaagt acagattctc ggggatacgc atggaaacgt catccatctt
    35281 tttgaaagag attgttctgt tcagcgccgt caccaaaaag tagtagaagt agcgccttgt
    35341 gtctctatga atgaacagca acgtcaaaaa atctgccagg cagctgtcca attgatgaaa
    35401 tatgtcgggt atgtcaacgc aggtacagta gaatttcttg ttgaaggaga tgatttttac
    35461 tttatcgaag tcaaccctcg cgtccaagtg gaacatacga tcacagaaat gattacagat
    35521 attgatatcg taacaacgca actgttgatt gctcaaggtt tggatctgca taaagaaatc
    35581 ggattgcctc agcaagaagg aattaaatta aatggttcag ccattcaatg ccgaatcact
    35641 acagaagacc cgttgaataa ctttttacca gacacaggga aaatcgatac gtatcgttca
    35701 cctggtggtt ttggcgtacg tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca
    35761 ccgtattttg attcattgct agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc
    35821 attcagaaaa tggaacgttg cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt
    35881 ccatttatgc tcaatgtcat cacacatcct gaattccaat caggaaatgc aaaaacaacc
    35941 ttcattgata gtacagcaac attatttgaa tttcctcgac tacgagaccg cggaaacaaa
    36001 acgatgaaat atatcggtga aattactgtg aatggttttc caggtatcga aagtggcgaa
    36061 aaaccatttt atgaagaacc acgtatgcct aaagatctaa tcacaagaac tgattacgta
    36121 acggctaaga atgtattaga tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga
    36181 caggaaaatt tgttgttgac ggatacgaca tttagagacg cccatcaaag tctgctagca
    36241 actcgtgttc ggacaaagga tttcaagcag attgcacgtt tgactggaga aggtcttcct
    36301 gaactctttt caagtgagat gtggggggga gcaacatttg atgtggctta ccgctttttg
    36361 aatgaagatc cttggcaacg gttgagaaaa attcgttctt taatgcctaa tacgttgctg
    36421 caaatgcttt tcagaggttc aaatgccgtt gggtattcta attatcctga caatgtattg
    36481 gtggaatttg tcaaagaagc agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt
    36541 ttgaactgga ctccacaaat ggaaaaaagt atccaagctg tacgggatac tggtaaaatc
    36601 gcagaggcag ctatttgcta tactggcgat atcaacgatc caagtcgggc aaaatacaat
    36661 gttcagtatt ataaagacat ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca
    36721 atcaaagata tggctgggct gcttaaaccg caggcagctt atcgtttgat tagtgaattg
    36781 aaagaaacga cagatctgcc aatccatctg catactcatg ataccagcgg gaatggaatc
    36841 atcacttatt ctgctgcttc aaaagcagga gtagacatcg ttgacgtggc aatgagtgca
    36901 atgagtggga atactagtca gcctagcatg agcagcttgt attatgcact ggtcaatggt
    36961 ccacggctgc cagaaatcac gattgaaaat gcacagaaat taaaccatta ttgggaagat
    37021 gtgcgcatgt attacaaacc gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat
    37081 atgcatgaaa tgccaggagg acagtattcg aatctacagc aacaggcaaa agctgtcgga
    37141 ttaggtcatc gctgggatga aatcaaacaa atgtatcata ctgtcaactt gatgtttggc
    37201 gatatcgtta aagtaactcc atcatctaaa gtagttggag atatggcact atttatggtc
    37261 caaaatgatc ttacagaaga agatatttat gaaaaaggcg aaacactaag tttccctgaa
    37321 tctgttgtga ctttcttcca aggagaacta gggcaacccg ttggcggatt tcctaaaaaa
    37381 ttgcaaaaga ttattcttaa aggcagacct gcattgaatg aacgtccagg tcttttggct
    37441 gaatcagtcg acttcaacga agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca
    37501 aaacaagacg aagtattaag ctatttgatg tatccgcaag tctttttgga ctatcaaaaa
    37561 gcatataatc aatttggcga tgttacatta ctggacacac caacattttt ccaaggtata
    37621 cgtttgggtg aaacaatcaa tgtacaaatc gaacgcggaa aaacgcttat cattcgttta
    37681 gatgaaatcg gagaacccga tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga
    37741 caaagacgtg aaatcactat caaagataat tcgatcatca gtacggtcca aacccgattg
    37801 aaagctgaac cgacaaaccg agaacaaatt ggagcaacga tgtctggttc tgttttggat
    37861 gtattagtga aaaaaggcga taacgttaaa aaaggtgata cgttgatgat tactgaagcg
    37921 atgaaaatgg aaacggccat cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg
    37981 tcttcaggag acactatttc gtctggtgac cttttgattg aagtgactga gaaataataa
    38041 gcgaaaggtg gggaaacatg aaaagactgg gtttgttttg gctgattctg ctattagtta
    38101 ttgtggcatt ctacatggaa ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact
    38161 ctgatcaaca aatatcaaac caaatgacaa cgtggaaata ccaggagctt aaagcaactg
    38221 gtttttctac ttatatcgga aaaccgatcg atcagctgga aaaggatttt ggccaagcct
    38281 atgatcgatt aactagcgga tttggttttg aaacaagata ttatgaaaat aaagctagtc
    38341 atctcacatt tgaagcgaac atccaagaca gcagagtcac aactgttaaa gtactgcaaa
    38401 cagggcctgc tgatatcgcg ccgttttatt tagggatgac gatgcaagat cttacgaaaa
    38461 tcactactat ctatactaat ttcacttttg agtaccatga tacagaagtt ggaatcgaac
    38521 taatggaaga ggatatgaat tatcgtccgc taattgcttt tgataacgac acttttgcta
    38581 ttttattttt tgatcaaaca acaaacggcc tattttcagt tgtgtattta gataaagata
    38641 gtcttttgaa actattgcca tatcaagtgt ttggtgaagg ccttccacat tatcaagcag
    38701 ataaatcggc tgattgggaa tcaatcaatc gcgttaaaga acgaaaaagt acgactttat
    38761 taaatatgct tagaagagaa gacgaacttc ccatatacaa tcaaacactt gcctttcaaa
    38821 accacagtca gctgctacta catgattttt tgacaaggcc tgaggaaata ttgtcagagg
    38881 aacgtatggc tgattggcaa aaatcgctag gcagtgctca aacgacagtg aaattctcac
    38941 tgacgaatga tgaattggag aagttaatca gtaaacaaaa attagaaaat actagtgggc
    39001 tatttattca tccggcgatt gacgctactt tttcgttttt atattggtac agcgatcctt
    39061 acaaccatga ccgtttcatg gacccgggaa cggattctgt aggcattgct ttttctaaag
    39121 aaaatatgtt agtcttattg caagaagaaa acggagaaag tagtgatagc agtgatcgtt
    39181 aacgaagagc tgtttcaact ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag
    39241 agtaaaacga tggcttctta tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt
    39301 tgccagttga aagaagcgtt cttgtctaag aaagaagcat ttgaaaatgt agcggcatat
    39361 ggtgagtatg cacctgatta tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag
    39421 cttgatatgg atgagcatgt tgcagctttc cgatatagtg aaacacagct gcaaagtctg
    39481 ttggatcaaa ttgtccaagt acttgccgaa gaagtttcta atgaaatcaa agtagatgca
    39541 ggtagcccgt tttttgaaac gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt
    39601 agacaatagt gaatttgaaa taacaaaacg acgcgggctg atcgtatggg tctacagctt
    39661 aaaacaatta aagaatctga aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa
    39721 atatgtcgtg ctttacttaa atgaagagac attggaagaa acagaagaac gaatccgtaa
    39781 attacatttt gttcgaaacg tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc
    39841 cgaaaaaata ggaaacaaaa cagtattaaa agacgaaggt ttcgaaattg aagagctaaa
    39901 tacagaaatc cgattagctg aacatcttta aaaatctctt atgagcagtt tttaaataaa
    39961 aaataacttg gataacgatc gatttttgat cattgtccaa gtattttttt ggtatactag
    40021 taccagtacc atttctaagg agtgagtaga tgcggataat atcaggaaat tatggcgggc
    40081 gccgtttaaa aagtttagca ggggcgaaca ccagaccaac tacagataaa gtaaaagaat
    40141 ccatattcaa tatgattgga ccatattttg atggagagac agtattggat ctgtttgcag
    40201 gaagcggtgg tttagctatc gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag
    40261 acaaaaatta ccaagcgcta aaaatcatca aagaaaacat cgaaattacg aaagaaccag
    40321 agaaatttac cacaataaaa cttgatgcgg acaaagcaat cgaacggcta gcagcggaga
    40381 aacaacaatt cgactacttg tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc
    40441 aaatccataa aatggaagaa ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa
    40501 cggataaaac agtcgaattg ccagaaactg tggctggatt ccatcaaata agaaaacaga
    40561 tttatggcat aacggccgta actatctata gaaaagaggg aaatcaatga aacgtgcatt
    40621 attcccagga agttttgatc cttttacaaa aggtcactta gatacggtgg aacgtgcggc
    40681 taaattattc gatgaggtag taattggtgt ttttatcaat acttcaaaga aaagtttgtt
    40741 tcccccagaa gaaagaatga cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa
    40801 agtcatgcat caagaaaatc aattgacggt agaaacagca aaagagattg gtgcagatgc
    40861 gttgatcaga ggcatacgaa gtatcaaaga ctttgaatat gaacgagaga ttgctcagat
    40921 gaatcatcat ctatatgatg aattggaaac tgtcttctta ttagcaaaac cagaatatag
    40981 tcacgtgagt tccagtattt taaaagaagt actgcacttt ggcggagatg tgagcagcta
    41041 cttaccgcca gtgatcaacg aagctttagc tggaaagcgt gaatcaaatg aaagctaaac
    41101 aatggttgaa aacagtactg attatcatat ttggaatgtt gatcattgga tttgtagtag
    41161 ttcccttgcc gtattatgta gaatcaccgg gagccacgat cgatctgaaa gaactaatta
    41221 cggtcaatga taaggaagat aaaggttctg ggtctttctc tcttacctct gttggtatca
    41281 ggcaagctac aggtttctta gctttaaaag ctaaaatgac cccttttgaa gaattgatca
    41341 gtgaagaaga attgactggt ggggcgacaa atgaagaata taatcaaatg caacagtatt
    41401 atatggattc ttcccaaaat gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt
    41461 ataaaatgga attcaaaggc gtatatgtca tgagtgtaga gaaaacatcg aatttctttg
    41521 gcaagctttc agttggagat acagtaacaa aagtagacgg gaaaagcttt caaagtacag
    41581 aagcatttat ggattatgta aaaagccaaa aagttggaca aacaatcgaa atcagttatt
    41641 tgcataatgg tgaagaaaaa acagcttctg gtgatctgat cgagttacca acagataaaa
    41701 aggcaggaat aggtatcacg cttactgacc ataccgagat cgaatcagat acagatgttc
    41761 gtattaattc tggctctatc ggtgggccat cagcgggatt gatgttcact ttagaaatct
    41821 atgaacaagt cactggtaaa aacttgcgac atggtaagca aattgcagga actggaacga
    41881 tcaacagtga aggagaagtt ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg
    41941 ataaagctgg cgtagagatt ttctttgcac cagatgatac gatttcagac gatgttaaga
    42001 aagaatatcc tgaaataaaa accaactacg aagaagcaaa agctgccgct aaaaaattga
    42061 attcctcaat gaaaatcgta ccagtaaaaa cagtacaaga agcgcttgac tatctagaga
    42121 aaatgaaatg aaactaaata attaaagagg ggttgtgaca cgagttctgt cacaacccct
    42181 ctttttcgtc aaaaatcttc acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta
    42241 gatgttgaag ggacgatttt gccagagctc actgtttctt caattttaaa tgaaaggagt
    42301 aatgaatgat ttttactggt cattctttga aaaaagaaca atgatagaaa aaatcagcaa
    42361 gaaaatacta gcttcaccca aattgatcat ttggatgtct atgatagttc ttatcttttg
    42421 tatcgcaagt ggttttttat tatcaaattt gctattttat agagaaacag aagagactgc
    42481 tgcaaaagaa atactttctt cgtttagttc cagtggagaa gatccatcag ctgaaggaaa
    42541 cgatgtagaa gaggatgctc gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa
    42601 ggagcctggt atctattcct tttcttcaga agaacgggtc tatgatgtac taaagcgtgc
    42661 aggtggatta ttagaagaag cggatagcga tcgaatcaac ttttctgcga agatagaaga
    42721 tcagcaagtc ctatatatcc ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt
    42781 ggcatcctcg gaaggaaaac agcctacagc agattccgag cctcccaaaa taaatatcaa
    42841 tacagccact ccatcagaac ttcagcagat tcctgggatc ggatcagtca aagcgcaaga
    42901 aatcattcga tttagagaag aaaacggatc cttccagaaa gtggaagacc tgcaggagat
    42961 ttctggtatc ggtgaaaaga cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa
    43021 tgattcaaaa tcgactggca cgagcaataa atggacgcta atggatacta agaataatcc
    43081 aattctaaga gcttggcagg acgaggaggt agtatttttg gataggaaat agcttgattg
    43141 ttcgcaaaga aacattaaaa aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg
    43201 ggattcatat tatgaatacg gctgtcagcg gcaataacag tcaagctcta cgagaacgct
    43261 ttgccaaaga tgtacttagc tatacagcag ataaagtatt tattctcatt gggacgaatg
    43321 atctagcaga gcataaacaa ctttctaaag aaacgtacca aaaaatctgc agtggatgat
    43381 agttcagctg aaaaaagact atagttctga tcatgctttc tttatcacgc caccagcagt
    43441 cgatgagaca aaacaaaaat atcggaataa caagttgatc aaagaatata tctctgttct
    43501 cacagaagta gcaaaacgcc atgattgtga agtatcgatt tttatacgga atttcttgaa
    43561 caaatggatt cgaagaattt ttataatggg ctaatggatg acggacttca tttcggcagt
    43621 gaagggtagg atcttttgtc agacaggatg attcgttata tttaaagaat attttgttta
    43681 agcaatgagt actttttaat caacttgaaa aaacggagga atataaaaca tgttagaaaa
    43741 atcttcaaaa gcatgcgata cgaataaaga aaaagttact gataagtcac ataatgaacg
    43801 cattccttgg gaccagtact ttatggctca ggcagtactg ttatctttac gtagtacgtg
    43861 tacacgttta gaagtaggag cgacactcgt tcgagaaaaa aggatcattg ctggcggcta
    43921 taatggagca gtcagtggag atgtccactg catagatgaa gggtgttacg tcgtggatgg
    43981 acactgcttg cgaacgatcc atgcagagat gaatgcactc ctacagtgtg caaaacttgg
    44041 tatcccgacg gaaggttcag aaatctatgt cacacatttt ccatgtttgg cctgtacaaa
    44101 ggctttgttg caagctggtg tgaaagggat caattatctc catgactatc gaaatgatcc
    44161 ttatgcccaa gcgttgatcg aacaattagg tgtatctgta caccaagttg ctttagactc
    44221 acagcatttt tcaaaacttc aaaatgaatt aacaggaaaa catgtctagc gttttagaaa
    44281 ggcatgttcg aaatcggttg atttttccgg caattacgtc gatgattatc tgtttgttgt
    44341 attacactga caattggggt tatcgtttgc ttcttgccgg aattttttta tggttttcgc
    44401 taaaagaagg acgttttatc gcgtacttgt gtattttgtg tagcataacg gtatccattt
    44461 cttataggct acaagagtat tcatttttag aggaaaaaac aacatctgcg caaattactg
    44521 tatttacaga tacgattcgt gcaaatggta attttttgac aatggaagga aggttatcag
    44581 agaataaaca aaagctccag ctttcttatc aagtaaacac agaagcagaa ttgaaaacat
    44641 ggcttaactt ccgaggaaag ggaatagaat tgactgctga aggcgagttc atctctccag
    44701 caaaaaagcg gaatctttat aattttgacc aagaagctta cttcaggaac catagaattt
    44761 ctggacggtt ccaaattcaa aagattgaac agatcaccat gcaaaatcac tggcgaaact
    44821 ggcttcagag aaaaagagga tcttttatct cctttgtcca atctgaattt ccgcaaaaaa
    44881 caagtgtata tattaattct ttattatggg gctataaaga tgcaggattc agagaaccag
    44941 aagaaatttt tagggagagt ggtttgctgc atctgttcag tctatcgggt ctacacatcc
    45001 aattttattt agggtggctg tattatttat ttcgacgtat cggatggcca ctacacgtca
    45061 gtattattcc gttgagtcta ctaactatct gttatgtttc tattgcagga agttctattt
    45121 ctgttttgcg tgctagtttg ctgtttttat tgcgattgct agtcaaattg ctgaataagc
    45181 attattccac catggactta tttgcagttg tcttttggct tcttttacta tttgatcctc
    45241 gatttctttt tcatgcgggt ggacagctta gttttttcat gagttttttg tttatcctga
    45301 tttcttttga tggagattgg ttgaaaaaaa cggcctctca cgtcctgttt actatgatca
    45361 cgatgccgtt gattgtatgg catttttatg agtggcctat tttaggtagt ctttttacat
    45421 tactgattgc accggtattc aagtttcttt ttcttcctgg tgtgttattt ctcacactct
    45481 tcaatcgctg gctgccggaa actttacttt tgctgataga agagggatta atcttatttg
    45541 agcgaataat cgatttttct tcagggtcgt ctctcattat aggacgtttg ccccttgtgc
    45601 tatctgggat gtatattttc ggattactga tttgtatgga taggttgaaa gaacaacctg
    45661 tgaaacactt gttttatgtt ggtctttttt cttttatttt tttgactttg ccatttttac
    45721 gcttttcttc aacgatcgct tttatcgatg tcggtcaagg agacagtatc tttttgcaaa
    45781 gtccttttcg aaaagaaacg attttgatcg atactggagg tcagttacac tttgaaagag
    45841 aaaagtgggc gcaaggtatg atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa
    45901 aaggtcaggg gataagtgta atcgataaac tcatcttgac acacgacgat acggatcatg
    45961 tgggagaatt acccacgtta tctcagcatt ttactatcaa aaaaatttat atcggttggg
    46021 gcgctggacg aaatgagcca ttacaaaggc atttatctga agctcaaaaa aatggtactg
    46081 agatcattga aatcaaacat ggggacagga tcagcggtta ttatgatctt tatgtattga
    46141 ctccttttga aaagggagag ggaagaaatg aagattcgat tggtctatgg atggaatata
    46201 acaataggcg ctttttattt ttaggagatt tgaatcaagc aatggaaaaa caattgcttt
    46261 tgatttatcc taatctgaaa gccgatgtag tcaaattagg tcatcacggt agccgtacgt
    46321 caagtaacac agattttctt tcccatattg aagcaaaaca tgggattata tcctgcggcg
    46381 taaataatcg ttatggtcac ccgcacagcg aagtattgga gacactagaa gaaaatcaga
    46441 taaaaacatg gcgaacggat caacaaggaa tgatcaccta tcgctggcat ccagtttttc
    46501 atcctcatgg gctagtggaa acaatgatag actagtaatt ctcatctgct catggtagga
    46561 taatttttga aagtggtgag taatttgagt atacaagcag agttacaaaa aatacgtaaa
    46621 gaaaaacttg catcttgtta cttgatttta ggaacagaaa agtttctcca agatcaagtt
    46681 cggacagaaa ttgtcaaaaa aatccaatta gagggcgaag atgacttaaa ttttttaagt
    46741 tttgacatgg aaaatgcaag tttagatgag gtagtagctg aagcagagac gttgccgttt
    46801 ttcggagatc aacggttagt attcgtagag aatccttatt ttttaacagg tgaaaaagta
    46861 aacaatggaa tcgaacaaaa tactgattta ttgacggctt atttgaaaga accattagaa
    46921 tcgactgtat tagtattctt tgcgccatat gaaaagttgg atgaacggaa aaaagtaacg
    46981 aaacaattga aaaaaacagc aattatcatc aatgtccaac agttgaatga aaaagaagta
    47041 cgtcaatatc tgatgaatac cttagagaat tcctcaatca aaatggaacg aagtgcaatt
    47101 gatttattct tgaggttgac cgacttagat ttatccaaaa tgatgagaga gttacagaaa
    47161 ttggtccttt atggacaaaa tcaacagcaa atcacggtaa gagaagtaga agaattagtt
    47221 cctaaaacgt tagaacataa tatttttgac atgacccaat atattttaaa agggaagacg
    47281 gaacaagctt tgcgtttata cgaagattta gttatgcaag gggaagaaac aatcaaaatt
    47341 aatgcgattt tgctttctca attacgttta tttttacaga caaaatttct tgtaaagatt
    47401 ggttatcagc aggcaaatat tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt
    47461 gccatgcagg aagtaaaaaa attcgatgaa catttgctgg tacagttgtt tgatcaatta
    47521 gttgagatgg attatcaaat caaaacggga caaatcgaaa aagaattaag ttttcaacta
    47581 ttcgttttgc gcacagggca gttgttaaga aatcgataag aagaaagacg agggcaaagc
    47641 atgcgggaag gtcgtttgtt tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc
    47701 gattctttta tttagcgatt tttttgctta aacgagattt gtcgcggtta gctttgtttt
    47761 tgtggattag accttttgat tcagccatat caatagcttt aacagcttct ttatataacg
    47821 catctacgtt gtcagcacct gaagctacag catcttcaaa ttttttgatt gcagtacgca
    47881 tagcgcttgt ttgagatgaa tttttcacat tagcgttttc gcttgtacga acacgtttga
    47941 ttgcagattc aatgtttggc attagtttca cctccgataa cttaagttct tacgcgtgtt
    48001 accgcataat tcaacattca ttattatacc taaaggcagc gtgctttgca atatgaacat
    48061 gacaagtttt tttccttaac agtcttttat taggattttt tttcagtaaa aacggatagt
    48121 acaagctgaa aaaaatcaaa aagacgctat tgtgggaggc aatagcgtca aaccaaacta
    48181 gatttttatt tatctattta aacaaagttt gaatgggaat gtttggtact aagttattat
    48241 ataacaaaga gaaccgtttg caaagttttt tttgaaaagg gatacgattc cctgttgttc
    48301 tttgtttttc tattaagaaa ctgcagttat tttataacca gtatcctttg atcagtaaag
    48361 gctattctgg tgtaccgatt attttaatgt attcatcgat attttttatc aacatttttt
    48421 ttatgaccaa ctggttccga cggttccatt tgccacttac ttccagcaca tatttattta
    48481 ggggcaaacg taagatatat tttgccaaat agcgatcttt aagcaggcca ttgatcacac
    48541 ggtcctcaag ctgcaagttg atcccagtca gcatcattgg ctgttcttgt aaaacgtacg
    48601 tatcaattaa aagaccttca aaagtttcca caagcgtctt cctttctttt caaagtaacc
    48661 tgaaaacagg cactaattct tccattttga tattcgttga agtggatgcg atcattcttt
    48721 cttttgtcca ctgtaatcta gtataacaaa aaagagcaga agagccaaaa agaaaaaaga
    48781 gcatcgaatt ctataggaag tctcattggc acggcctaaa ttattacaaa acattgatta
    48841 aaaaaagccc ctgcatcgca ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag
    48901 cagtatcaaa cacattttgt atcaaatcag gaaataatag ataagctaag cccagaatca
    48961 aaagtaccgc tagaagaaac cagccgattg ttcctatgaa atactttaat ttttcgaaca
    49021 ttgtatcacc tcgttgtcag tatatcacgg atcagtaaat tgggggtgtt aagaatctcc
    49081 taaaatagac aaaatttgtt ctattttcct aaagcaaata cttgtaagat acagtaagtg
    49141 ataccagaag caatgaccgg tccggcggcg attcctctta agaagacaac acctaaaatc
    49201 gtgccgaaaa caagtgcaac agtgatttgt ggatcagaag caatcaatcc tacacccttg
    49261 gaagataaga cagccactag cccaccgcaa aaaacagcta cccagccaat aggggattta
    49321 aaggcttcta atagatcacg tagaccgatt tctcctgttg cgatagggat tagaatagcc
    49381 gctgtgatga tcgtcacccc aatattgatt cctttgcctt gaagccaagg gaaaaaggaa
    49441 cgggaggctg gtatgagctt cataatcaaa acaaaagctg ccgctatcat cagactttga
    49501 tttttagcta aaattgctac aagtaaaata attcctaaaa aaagccaact ttccatgaaa
    49561 tatattcctc actttcttcg aaacaatcat aacatgtttt tagaaagtta caaaagtagg
    49621 aattcatcct tcaagaccta ttcataagct gtttttttca taaaataaac aaaatcataa
    49681 aaaaacactt tgcttatttg acagaagaca gccgcgcttt gtatagtgga caagacttac
    49741 atttttttga agaatcagaa agatcaggta aaggtcttag taaacgcaag ccattcagaa
    49801 taaccaatat tgtgcttcct tcgtgtccta caactccaag aggtagattt aaaaattgca
    49861 ggaagttaga agcaatcaac aagagaataa caagaatcga aaaaataata ttttgtttga
    49921 tgatccgccg catttttaaa gatagccgat gactgtagac aaatttattt aagtcgcttt
    49981 tcattaagac gatgtctgcg atatccatag cggcatctgt cccttgcccc atagcgacac
    50041 ctaaagaagc ggctgctaaa gcaggcgcat cattgactcc atcaccaacc atcgcattta
    50101 cttggtaaag ttcattctct tttttgacta gttgtgtttt ttcttccgga gtacagcttg
    50161 catagtaatg ttcaatccct acttgttcag caatggcctt tgctgtgcca gaatggtcgc
    50221 cggttatcat acttgtatgg atgttttggg atttaaagta ggaaattact tgttgggtat
    50281 ttgcttttgg aatatctagc agacctaaaa cagcaattaa ctgctgatct ttagaaagat
    50341 aaatcacggt tttgccttga ttttccaaac gttcgatcat ttccgcaatc tctttgctga
    50401 ttatcatcgt ctcaggatca tatgcgtgct tgccaacttt ccattgcgca cctaaatagg
    50461 tagtttgtaa tccaaaacca gtaatttctt ctattggtaa gtattggatt tcttcaggta
    50521 ttgtgcaatc gaaatattgc aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca
    50581 tagcaacaag aagttgttgt gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa
    50641 ctggttttcc tttagtgagc gttcctgttt tatcaaaggc aatcgctttt aattcagaaa
    50701 gcttttctaa atggataccg cctttgatta aaatcccatt ttttgcagaa tttgaaagag
    50761 cagctagaga agctggtgta gcagatgcga caagcgcaca aggagaagcc acaactaata
    50821 ataccattcc tcgataaaag ctttcatccc aactccaacc taggaaaaag tgggggagaa
    50881 ggatcattag aggaacagca agcaaaatca attttacata attattttcg attcgttcga
    50941 taaatgaagc tgtttgagtt ggcgtattct gcgcttcttc tactaattgg ataatcttag
    51001 caaagacagt ttcggaactc gtttttgtta ctcgaatcgt gatgggattt ccttgattca
    51061 gcgttcctgc atacatctca tcgcctaaat atttttctac aggcacagat tcaccgctta
    51121 atgcagactc atcgatcgtt gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg
    51181 ctcctttagc gacaaatacc aagttatcca cctttagttg agaaacatct acttcttgca
    51241 tttgtccatt ttcttttaat aattgggctt tttgtggttg aagattcatc aaagcagtaa
    51301 tttctttttg acttttatta gttgtgtatt cctctaatgc accgcttaaa caaaagatga
    51361 atgtcaacat tgctccttca aagtaattac cgatcaaaca ggcaccaata gcagctaaag
    51421 ccatcaataa atccacattc agtgtccgat cattccacaa ttctttcaag ccttccgatg
    51481 tttgtttata tccaccgatg ataatggcac tgatataaaa aagtggggtg atggtaagag
    51541 agaatcggtc gaagataaaa ccgaagatca taaataaaag gcagccgatt gttgctgcaa
    51601 aagctttttt ctgattagtc atcgttatta gacctccttg tcaaaacaag gatagcataa
    51661 taaaaatgta attgataaaa ataacagcta aatgagaacg ataatggtta tcatttggtg
    51721 attaagcttg atgttgtttt tattatttat aatgattatt attaaagaaa ataaaaaacc
    51781 agaacatttc tcaaataaga gaagtgttct ggttttttct acattgcaat aaaccaataa
    51841 ccaatcaaaa tgataccaag gataatacga taccagccaa aagcggtaaa gtcgtttttc
    51901 ttcaaatagc ttagtaagaa tttgatagct aagacggaaa ccacaaaaga tacgaatgtt
    51961 cctacaagaa gaacaacagt ttcttccatt ttgaatgaat tcccttcagc taaatatttg
    52021 acgatcttca agccgcttgc gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct
    52081 gtagcgacaa aacgcgaagc accaatcaag atagatccta ggatcgttgc gccagaacgg
    52141 gatgttccgg gaacaagtgc caagacttgg aataacccaa caagaagagc tgcttggtac
    52201 gtaaagtcat tcagagaagt ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata
    52261 ataaaggcaa taccgtaaac gatcaaaaca atggcaactg gtaagaactt gtggaaattt
    52321 gcttctaacc aatcatccaa aggcaaacca atgatgatgg ctggtatgca agcaacgatc
    52381 actttaaacc aaagtgtcca cgtatctttt ttttctaccg ttgatttact tggagaaaat
    52441 gggtttaact tgtgaaaata aagaacgacg acagccaaga tcgcccctaa ttggataacg
    52501 acattgaaca tcgacataaa ttgtgaactg gcatccagtt tgatgaattc atctgctagg
    52561 atcaaatgac cagtactact gatgggcagc cattccgtta ttccttcaat aataccgaga
    52621 atcactgctt tgataatatt tgcaaaaaac ataaattcct tccttcccaa ttaagataca
    52681 tcttatagta taccgttatt ttttgagaaa cccaatctgt tttttaatat taagaaaaac
    52741 ttgagaaata aagcagataa gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga
    52801 ctttgccttt tatgattctc tggtaaaaat taaaatatcc ccattataga aacgagtcgc
    52861 gactatgtgt tttcccgtta tcgatataag acgtatcatt gatgccgtgg atagaaaatc
    52921 gaccgtttgc ataagtgacc tttgttacgc tgccatttaa aagcccagca cgaacaggct
    52981 gtttaggatc gatcagatta agcaaaaagg cgatcgtcaa accatgagat acgactaaaa
    53041 catttccacc accatttttt tcgcagtgat gggcgatatc ttcaaatcct gtccatacac
    53101 ggtctcgcaa tgtttcataa gtttccgccc acccagcggt atctaagttg tatacagcat
    53161 tagcaatttg ttcaaaagaa acttctgtgg taaacatttc atcatatgtt ttaaaattca
    53221 atacacgagg gatcacaccc cacatttcca tgtcatatcc accttcaaga gacccgaaac
    53281 accattctct aatccttttt tcaataaaat aaggaatttc ttttccctta gaatgttctc
    53341 ctagaataat acgggcagtt tcgattgcac gaccactgtc actagaataa gcaaatgaaa
    53401 agtcgacatt ttttaacccg cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga
    53461 ggggtgtatc acaccagcct tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc
    53521 gtacgatata aagtgtagtt tctgtcattt tattctcctc ctaagtcgtt ttaacgattg
    53581 aaaaaaggat ttgtcctttt ttcctgttca atcgtcgttg catcgccatg tcctggatag
    53641 actgcaaatt catcaggtaa tacaaacagc tgggtccgaa tactatgaag taattgttct
    53701 aaattccctg tatacagatc ggttctaccg atacttcctc taaacagggc gtcgccagaa
    53761 acgacaaaat catcaaaaat aaagctgacg cttcctatag aatgccctgg ggtagggacg
    53821 acgcgaaaag acatgtttcc taaagtataa gttttcattt caaattcata ttctgcaggc
    53881 gagacaataa catcagcaat atcatcatgt ctaccgagtc cagaaagatt caaaatcgga
    53941 tcacctagcc atttttgctc tagtgggctg acataaactg ggatttgata atacgcgcgt
    54001 aaaggttcca ctgcaccgat atgatcgtag tgagtatggg taagcaaaat cgctaccggc
    54061 tgttggtttg tttttttgat ttcttgtttg attcgttccg catcttctcc agggtcaatg
    54121 atcaaaagtg cttcttcgtt gtagaccaaa taacagtttt cttcaatcat tcctgttttt
    54181 aatctttcga tatgcatgtg cattcctcct tgatagccaa aacttctttc atccattagt
    54241 ataacgcatt cattgctagg aaacaaaaac aggtacttat ttctctttac ttatctgaat
    54301 tatcagacta tatgaaaagt atttggatat tctaaagaga aggaaagtaa gttttcatag
    54361 cattttaata tgtccttgtt acattagatt catccaaaaa tccaaattct atatcctata
    54421 tccctgccta atcagcaggg tgttttttta tgtttggaaa gagagctctt tttgctaaac
    54481 taggtttttt caggtaatct tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa
    54541 atatctgtgc ggcacttagt agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa
    54601 aaaagtaacc atactgcatt agaaggagcg agaatccacc gcaagctcca aaaagatgcg
    54661 ggagaaaact atgaaaaaga agtcttttta aaaacaacag ttcaattaga tgaatattac
    54721 ctgatcgttg aaggccgtgc agacggtatt ttcaaaagag acggtattta ttatatagat
    54781 gaaatcaaaa cctccgaacc acgatttgaa gatttagaac cagaacaagt ggaacttttc
    54841 tttcatcaag caagggtgta tgcctatatc tatagtcaag aacatgattt acaagaaata
    54901 aatctgcaat tgacttactt ccagacggca gaagaagtga tcacgagaaa ggttgaacat
    54961 cagacaagag agcagttaga aacttttttt aaaaagctga ctgaagatta taaagaatgg
    55021 cttgtttttc aagaaaactg gcgcactgta cgcaatgcct ctttgatggc tttgaaattc
    55081 ccccatgatg agtataggaa aggtcagcgt gaactagcgg tagctgctta taaaacgatc
    55141 cgtacgaaac agaaattgtt tgtagaagca cctaccggaa caggaaaaac aatctctacg
    55201 ttattccccg ctttgaaagc tgtaggggaa gaagagggag aaaagatctt ttatctaacg
    55261 gctaaaacaa taactagaca agtagctgaa gatgctatga cagcattaaa agatacagga
    55321 gctgaagtaa aaagtgtcac tttgacggcg aaagacaaga tctgcttttt agatgagacg
    55381 acctgtaatc cagatcagtg cccatatgcc aatggttatt acaatcggat taatgaaggt
    55441 ttatgggatc ttttgaatca tgagaatcag atcactcgtg aagtcatcga aacttatgca
    55501 agaaagcata cactttgtcc tttcgaacta tcattagatg tcagtatctg gtgtgacgta
    55561 atcattgggg attacaatta tttatttgat ccaactgttt atttaagacg tttttttgaa
    55621 gatgaaaaaa acgaggacta tctgtttttg attgacgaag cacataattt ggtgaatcgt
    55681 tcccgagaga tgtattcggc agaactatct tatgaaaaaa cgaaacgaag taaggaagct
    55741 ataccaaagg aattcaaaaa acttcatcgc cgtttcaaca aactattaaa agaatttgat
    55801 tcgatccgag aaatagcaaa agaagatcat tgggattatc atcatcaaaa agcaccggct
    55861 gaatctttag taaaggcagg ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa
    55921 tttcctgagc acccatcaca agaacaattg cttccttatt attttgattt gcttcatttt
    55981 ttgaaggtca gtgaatttta tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc
    56041 gatctgatag taaaagagtt ttgtattgat ccaagcttgt ttcttgagca gtcgttggat
    56101 aaaggaaaaa gcagtattct tttttctgct agtttttcac cactctctta ttatcaggaa
    56161 acactggggg gacaaaaaag tctggcttac aaactgccga gtcctttccc agaaggaaac
    56221 cagcaagttt tcatcgcaaa ttacctagag acgacttatc gaaaaaggga acacagtctg
    56281 gcaaaattag tagagacgat tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt
    56341 ttctttcctt cttatcaata tttagatctt gcggtggaag cattcagaca gcaatatccc
    56401 gacagcaatg tgctaatcca agaaacggac atgaatgagg aagaacgcga acgattttta
    56461 acaaaatttc aaacaaatcc cgctgaaaca ttgattggat tttgcgtact tggcgggatt
    56521 ttttcagaag gaatcgattt gagaggggac cgattgatcg gcagtatgat cgtaggtgta
    56581 ggtttgccgc agatgaatca tgagcaggaa ctgatcaaat catattatga tgagaaggaa
    56641 caccttggat ttgcttacgc ttatcagctt cctggaatga acaaagtctt gcaagctggt
    56701 ggtcgagtga ttcgtggcat ggcggatcaa ggaatcgttg tattggcaga tcaacgattt
    56761 tctagttttt cttatcgacg tttattccca caacattggc aaacagcaag agaagtacga
    56821 agtgttgggg aattggaaga aggaatacag cgtttttggt tatcaaaaga agcagtaaat
    56881 caaaaaaacg aggaatgaca gacgtcagcc tcgttttttg attaacgata gttagtgaat
    56941 tgcaactcga ttggtaagtc aagtttacgt aataaagcaa tcacagcttg tagatcatca
    57001 cggttcttac ctgtcactct aatctgatct tcttggattt gtgcttttac tttcagtttt
    57061 tcatttttga tagctgtagt gattttcttc gcgttttcac gatcgattcc gttgaccaaa
    57121 tcggcagttt gtcgagcttt gccacctaga gcgtgttctg attcggaaaa atggatattt
    57181 ttgattggca catttctttt gatcagctta ccaaataata catctttgat ctgttcgatt
    57241 ttgaaatcat catcgctcaa tacaactaga ttgctgttct ctaatttgat ttctactgta
    57301 gaatccttga agtcaaagcg attggacaat tcttttgttg tttgctgaat cgcatttttt
    57361 acttcttctg tattgacttc tgatacaatg tcgaagctag catcttttgc cataggaacc
    57421 ctcctgatat acatataaat atttaccata catctatctt atcataggat tagggcagat
    57481 tcttacctgt ttgctaaaat tcttctcatt tgagttcgaa gtattttatt ttaatagata
    57541 aactgttatt cttacatttg gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta
    57601 tgactgcgta tcataaaaat gaaagattaa agaaaatagt ggttattttg ataaccggat
    57661 tgaccgctgc ggtcgcttta aattattttt tgattccagc caatgtcttt tctgcaggga
    57721 tgaatgggat cgctcaaatc atttcctcgc ttttatatga atggtttcat atcaagatag
    57781 acacaggaac atttattttt cttttgaata tccctgtgtt tgtattaggg tttctcaaag
    57841 taggaaaacg agcaacgatc cttagttttg tgaatgttgt atgtgtatcg gtgatgacga
    57901 tgctgcttcc agtcggacag atcactgata atattttgat gaatgcccta gtaggcggtg
    57961 ttttattagg tcttggcgta ggattatctt taaaaatggg ctttacaact ggtgggatgg
    58021 atatcatctc tcttgtactt tcacaaacaa ctggaaaaac agtcggaaac tacatgctga
    58081 ttctaaatgg tatcattgta gtcatcgcag gcttcttctt taactgggaa agtgccttgt
    58141 atacgattat ttccatttat gcaatgactc aagtaattga tgccatccac acgagtcatc
    58201 aaaaagtaac agcaatgatc gttaccgtca agccagaaga ggtaacagaa gaactagcaa
    58261 aacggatggt acgtggaatg acattactgc cgtctgtcgg tggctattca aaaaaacaag
    58321 ggaacatgat catgatggtc atcacccgct acgaaatgta tgatcttgat cagatcgttc
    58381 atgaaatcga cgacgatgca tttatcaata ttttaccaac tcattcagta tttggccggt
    58441 ttgcaaatga aaacgaacaa aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa
    58501 atcgttcaca aaaaaaatga ggaatcaggt ttattttaga ataaatttaa tctattacat
    58561 ctaataagaa catttttata aatttaaacg agaatcttac ttctaaattc tcatttacaa
    58621 gcagctaaaa aaagagtatg ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg
    58681 aagcaaacca agaacggtct ggacaagaaa tagaactaat caaagaacgt attcttgctg
    58741 ccttggaaat ggtgattgat cctgaattag gaatcgacat cgtcaattta ggcttgattt
    58801 atgatataga attcaatcct gaaaatggag aaacagtcat taagatgact ctaacaacta
    58861 tgggctgtcc attagcagat attttaacag aatctatcca tgacgcatta aaagaagtgc
    58921 cagaagtaac gaaagcagaa gtcaaactag tctggtatcc agcatggacg acagataaga
    58981 tgagccgtta tgcacggatt gcgttgggga tacgatagaa atcttggctg actagctatg
    59041 agaagcaaat aggaaaaaca aaaaagcctt ttatcctttc tagaagaaaa gagataaaag
    59101 gtttctttgt ttataaaatt ttgtctgcta tattataagt taattaaata aatgtgttct
    59161 attgataaag gacattcaaa tccctttcct tttggaatcc ttctttttta catcagtttg
    59221 attttcgtat agtattcttt ctcatcttca cgataaatcg cagccccatt tttttgttgt
    59281 gctttaagac tgccaagatt ttttttattt acagacaaaa gtgcctcatc aatgccgatt
    59341 ttttttgcct cttgtagtac taatgctaat ccttgtgtgg catatccttt tttacgataa
    59401 ttaggggaga tgccgtaacc aatatggcca gcgccgtttc ttaatgtttc gttcagttta
    59461 tggcgcaaat taaaaattcc tacatactca tgttcatcac tttctaaaat aaagacggtt
    59521 gcgggaacgt aactctcagg aagatcgatt cctaaagacg ttttttcgca tttttttaca
    59581 taatctaaaa attcagcaaa tgtatagcca taagcattgt tgataaatcc actctcattt
    59641 ggtccaaagc tagtttgtag ctgataagct ttctttaaat ctgcttccca tagtttgatc
    59701 agtttcatcg tattcacctc tttaagtaac cttattcatt tgttttctac tatagcacta
    59761 ttgttgtaaa tagccatgcc tatttttatt tattgtgtca tatcgtatta agaatcatcg
    59821 aacaatacaa atatgaatta caataaaaaa attatttgaa gttcgttcat tcgttactta
    59881 tgatctctag aagaaagaaa acgtgaatta cttcagcgtc tagaattcca gaaggatagt
    59941 aatcaacaga agaaataatc aatatccaaa tgaaagaaga tagctgaatc gagaaaaaga
    60001 ggaaaaaaga aatgaagaaa aaaataaaat agttactaat cccagagtcg ttcgtttaac
    60061 attgaaatcg atcatatgga taaaacagat tttttcacca accctcaata gatgatggag
    60121 ttgctcggat gaattatttg tgtagatggc ataagggagg gcgtacagaa atgaaaaaga
    60181 acaaaaatat gttcttgaca aaaaacacat gtaatcctaa actaaaagat gtgatgcggc
    60241 tatggcggaa ttggcagacg cgctagcttc aggcgctagt gagggtgacc tcgtggaagt
    60301 tcgagtcttc ttagccgcat aatttgtttt tttataaaga gaaggtacac ctggagcgat
    60361 gagaaagaaa ataacatatt atctaaaaga atataggaaa aattttaaac aacggaacca
    60421 gattcaaata aatcaaaaaa aatttgtgtg aaggtgggac agtttaaaac ctaatagatt
    60481 gattcaaata gtagatttat agattctgat acaagaaata ctggcaaacg atagttaagg
    60541 aataaagact aaacttaaac tcaaaaagag gtcatgcgaa agaataaatc gcatgacctc
    60601 ttactatatt ttgaatgtgt ttaaacgttc aaataatgaa tcttagtacc aaccgtttgc
    60661 ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat attgttctgc
    60721 tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat ctaattggta
    60781 acggccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt tttgtgcgat
    60841 ccattctttt gcagagcttg tacttgctgg agtagcagca gtttctgtta cagcaggagc
    60901 aggttgtgtt tcagcaacag gcgctgcttg tgtttccgtt tgaaccgttt cttgtacaac
    60961 aggttgttca gcttgaacag gttcagcttg gactggtgct tcttgaacgg ctgcttgagg
    61021 ttcagcagct gcttgtgcag agccttctgt tgggattgtt aattgttgtc ctgagta
//
LOCUS       DAFGZE010000007        59452 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.007, whole genome shotgun sequence.
ACCESSION   DAFGZE010000007 DAFGZE010000000
VERSION     DAFGZE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.007"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(451..1761)
                     /locus_tag="LTU54_000449"
     CDS             complement(451..1761)
                     /locus_tag="LTU54_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL8295188.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="LTU54_000450"
     CDS             complement(1773..3191)
                     /locus_tag="LTU54_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8295189.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="LTU54_000451"
     CDS             complement(3431..4999)
                     /locus_tag="LTU54_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL8295190.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="LTU54_000452"
     CDS             complement(5003..5413)
                     /locus_tag="LTU54_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL8295191.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="LTU54_000453"
     CDS             complement(5410..6333)
                     /locus_tag="LTU54_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HBL8295192.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="LTU54_000454"
     CDS             complement(6330..7208)
                     /locus_tag="LTU54_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HBL8295193.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="LTU54_000455"
     CDS             complement(7195..8532)
                     /locus_tag="LTU54_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HBL8295194.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="LTU54_000456"
     CDS             complement(8535..9638)
                     /locus_tag="LTU54_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL8295195.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="LTU54_000457"
     CDS             complement(9712..11220)
                     /locus_tag="LTU54_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL8295196.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="LTU54_000458"
     CDS             complement(11186..11878)
                     /locus_tag="LTU54_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL8295197.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="LTU54_000459"
     CDS             complement(11900..12874)
                     /locus_tag="LTU54_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295198.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="LTU54_000460"
     CDS             13174..14046
                     /locus_tag="LTU54_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8295199.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="LTU54_000461"
     CDS             complement(14504..16702)
                     /locus_tag="LTU54_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295200.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="LTU54_000462"
     CDS             16934..17926
                     /locus_tag="LTU54_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295201.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="LTU54_000463"
     CDS             17986..19407
                     /locus_tag="LTU54_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBL8295202.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="LTU54_000464"
     CDS             19400..20899
                     /locus_tag="LTU54_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBL8295203.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="LTU54_000465"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="LTU54_000465"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL8295204.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="LTU54_000466"
     CDS             22491..23174
                     /locus_tag="LTU54_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HBL8295205.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="LTU54_000467"
     CDS             23176..24012
                     /locus_tag="LTU54_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL8295206.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="LTU54_000468"
     CDS             24023..25777
                     /locus_tag="LTU54_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295207.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="LTU54_000469"
     CDS             complement(26053..26394)
                     /locus_tag="LTU54_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL8295208.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="LTU54_000470"
     CDS             complement(26462..28633)
                     /locus_tag="LTU54_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL8295209.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="LTU54_000471"
     CDS             28802..29701
                     /locus_tag="LTU54_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL8295210.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="LTU54_000472"
     CDS             complement(29772..30629)
                     /locus_tag="LTU54_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8295211.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="LTU54_000473"
     CDS             complement(30614..33316)
                     /locus_tag="LTU54_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBL8295212.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="LTU54_000474"
     CDS             complement(33329..34618)
                     /locus_tag="LTU54_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HBL8295213.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="LTU54_000475"
     CDS             34768..35814
                     /locus_tag="LTU54_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295214.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="LTU54_000476"
     CDS             35816..37969
                     /locus_tag="LTU54_000476"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HBL8295215.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="LTU54_000477"
     CDS             complement(38442..39893)
                     /locus_tag="LTU54_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295216.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="LTU54_000478"
     CDS             complement(39890..41614)
                     /locus_tag="LTU54_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL8295217.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="LTU54_000479"
     CDS             complement(41607..42221)
                     /locus_tag="LTU54_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL8295218.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="LTU54_000480"
     CDS             complement(42566..44023)
                     /locus_tag="LTU54_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8295219.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="LTU54_000481"
     CDS             complement(44064..44984)
                     /locus_tag="LTU54_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8295220.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="LTU54_000482"
     CDS             complement(44996..45943)
                     /locus_tag="LTU54_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295221.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="LTU54_000483"
     CDS             complement(46422..47141)
                     /locus_tag="LTU54_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL8295222.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="LTU54_000484"
     CDS             complement(47190..48836)
                     /locus_tag="LTU54_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8295223.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="LTU54_000485"
     CDS             49015..49605
                     /locus_tag="LTU54_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL8295224.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="LTU54_000486"
     CDS             49697..51202
                     /locus_tag="LTU54_000486"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HBL8295225.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="LTU54_000487"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="LTU54_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL8295226.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="LTU54_000488"
     CDS             complement(52638..53156)
                     /locus_tag="LTU54_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295227.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="LTU54_000489"
     CDS             complement(53172..53498)
                     /locus_tag="LTU54_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HBL8295228.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="LTU54_000490"
     CDS             complement(53511..55490)
                     /locus_tag="LTU54_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL8295229.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="LTU54_000491"
     CDS             complement(55511..55825)
                     /locus_tag="LTU54_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HBL8295230.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="LTU54_000492"
     CDS             55993..56286
                     /locus_tag="LTU54_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBL8295231.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="LTU54_000493"
     CDS             complement(56364..57476)
                     /locus_tag="LTU54_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL8295232.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57629..57853
                     /locus_tag="LTU54_000494"
     CDS             57629..57853
                     /locus_tag="LTU54_000494"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295233.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            57863..58042
                     /locus_tag="LTU54_000495"
     CDS             57863..58042
                     /locus_tag="LTU54_000495"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295234.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="LTU54_000496"
     CDS             58236..58406
                     /locus_tag="LTU54_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8295235.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(58830..>59452)
                     /locus_tag="LTU54_000497"
     CDS             complement(58830..>59452)
                     /locus_tag="LTU54_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8295236.1"
                     /translation="QSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEI
                     ERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGAD
                     GKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDI
                     RGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17787 a        12134 c        10392 g        19139 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttccaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcact tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 tttccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcgattt
    59281 tctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gattggtgtc
    59341 gcatccaatt cataccccgt tggcgcttgg gtttctacca gctgatacac tcctggtgct
    59401 aagccgtcaa tcgccagttt tccatctgct cctgtggtta gacctgactg ca
//
LOCUS       DAFGZE010000008        56801 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.008, whole genome shotgun sequence.
ACCESSION   DAFGZE010000008 DAFGZE010000000
VERSION     DAFGZE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.008"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            470..856
                     /locus_tag="LTU54_000498"
     CDS             470..856
                     /locus_tag="LTU54_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8295237.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            950..1141
                     /locus_tag="LTU54_000499"
     CDS             950..1141
                     /locus_tag="LTU54_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295238.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1655..1987
                     /locus_tag="LTU54_000500"
     CDS             1655..1987
                     /locus_tag="LTU54_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8295239.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2283..2438
                     /locus_tag="LTU54_000501"
     CDS             2283..2438
                     /locus_tag="LTU54_000501"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL8295240.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2714..4054
                     /locus_tag="LTU54_000502"
                     /pseudo
     CDS             2714..4054
                     /locus_tag="LTU54_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4295..5911)
                     /locus_tag="LTU54_000503"
     CDS             complement(4295..5911)
                     /locus_tag="LTU54_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL8295241.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEVCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            6029..6247
                     /locus_tag="LTU54_000504"
     CDS             6029..6247
                     /locus_tag="LTU54_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295242.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6442..6903
                     /locus_tag="LTU54_000505"
     CDS             6442..6903
                     /locus_tag="LTU54_000505"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8295243.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(7096..7335)
                     /locus_tag="LTU54_000506"
     CDS             complement(7096..7335)
                     /locus_tag="LTU54_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295244.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7359..8882)
                     /locus_tag="LTU54_000507"
     CDS             complement(7359..8882)
                     /locus_tag="LTU54_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL8295245.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8900..9022)
                     /locus_tag="LTU54_000508"
     CDS             complement(8900..9022)
                     /locus_tag="LTU54_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL8295246.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(9052..10035)
                     /locus_tag="LTU54_000509"
     CDS             complement(9052..10035)
                     /locus_tag="LTU54_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBL8295247.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(10059..10208)
                     /gene="rpmG"
                     /locus_tag="LTU54_000510"
     CDS             complement(10059..10208)
                     /gene="rpmG"
                     /locus_tag="LTU54_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL8295248.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(10229..10606)
                     /locus_tag="LTU54_000511"
     CDS             complement(10229..10606)
                     /locus_tag="LTU54_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295249.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10638..10817)
                     /gene="rpmF"
                     /locus_tag="LTU54_000512"
     CDS             complement(10638..10817)
                     /gene="rpmF"
                     /locus_tag="LTU54_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL8295250.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10832..11101)
                     /gene="rpsN"
                     /locus_tag="LTU54_000513"
     CDS             complement(10832..11101)
                     /gene="rpsN"
                     /locus_tag="LTU54_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBL8295251.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11624..13390)
                     /locus_tag="LTU54_000514"
     CDS             complement(11624..13390)
                     /locus_tag="LTU54_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295252.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13350..15104)
                     /locus_tag="LTU54_000515"
     CDS             complement(13350..15104)
                     /locus_tag="LTU54_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295253.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(15214..15783)
                     /locus_tag="LTU54_000516"
     CDS             complement(15214..15783)
                     /locus_tag="LTU54_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HBL8295254.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15801..17216)
                     /locus_tag="LTU54_000517"
     CDS             complement(15801..17216)
                     /locus_tag="LTU54_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL8295255.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(17227..17895)
                     /locus_tag="LTU54_000518"
     CDS             complement(17227..17895)
                     /locus_tag="LTU54_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBL8295256.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(18025..18609)
                     /locus_tag="LTU54_000519"
     CDS             complement(18025..18609)
                     /locus_tag="LTU54_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8295257.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18940..19173)
                     /locus_tag="LTU54_000520"
     CDS             complement(18940..19173)
                     /locus_tag="LTU54_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HBL8295258.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(19188..20138)
                     /locus_tag="LTU54_000521"
     CDS             complement(19188..20138)
                     /locus_tag="LTU54_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL8295259.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(20135..20977)
                     /locus_tag="LTU54_000522"
     CDS             complement(20135..20977)
                     /locus_tag="LTU54_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL8295260.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(20979..21698)
                     /locus_tag="LTU54_000523"
     CDS             complement(20979..21698)
                     /locus_tag="LTU54_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL8295261.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22390..22548
                     /locus_tag="LTU54_000524"
     CDS             22390..22548
                     /locus_tag="LTU54_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295262.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            22620..23846
                     /locus_tag="LTU54_000525"
     CDS             22620..23846
                     /locus_tag="LTU54_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8295263.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(23934..24326)
                     /gene="rpsI"
                     /locus_tag="LTU54_000526"
     CDS             complement(23934..24326)
                     /gene="rpsI"
                     /locus_tag="LTU54_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBL8295264.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24342..24785)
                     /gene="rplM"
                     /locus_tag="LTU54_000527"
     CDS             complement(24342..24785)
                     /gene="rplM"
                     /locus_tag="LTU54_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBL8295265.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25304..25771)
                     /locus_tag="LTU54_000528"
     CDS             complement(25304..25771)
                     /locus_tag="LTU54_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HBL8295266.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25891..27429)
                     /locus_tag="LTU54_000529"
     CDS             complement(25891..27429)
                     /locus_tag="LTU54_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBL8295267.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27509..28195
                     /locus_tag="LTU54_000530"
     CDS             27509..28195
                     /locus_tag="LTU54_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBL8295268.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28396..28923)
                     /gene="msrA"
                     /locus_tag="LTU54_000531"
     CDS             complement(28396..28923)
                     /gene="msrA"
                     /locus_tag="LTU54_000531"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL8295269.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(29007..29684)
                     /gene="pgmB"
                     /locus_tag="LTU54_000532"
     CDS             complement(29007..29684)
                     /gene="pgmB"
                     /locus_tag="LTU54_000532"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL8295270.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29681..30811)
                     /locus_tag="LTU54_000533"
     CDS             complement(29681..30811)
                     /locus_tag="LTU54_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL8295271.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30811..32994)
                     /locus_tag="LTU54_000534"
     CDS             complement(30811..32994)
                     /locus_tag="LTU54_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL8295272.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32991..34016)
                     /locus_tag="LTU54_000535"
     CDS             complement(32991..34016)
                     /locus_tag="LTU54_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8295273.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(34061..34888)
                     /locus_tag="LTU54_000536"
     CDS             complement(34061..34888)
                     /locus_tag="LTU54_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBL8295274.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34885..35952)
                     /locus_tag="LTU54_000537"
     CDS             complement(34885..35952)
                     /locus_tag="LTU54_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBL8295275.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35978..36814)
                     /locus_tag="LTU54_000538"
     CDS             complement(35978..36814)
                     /locus_tag="LTU54_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8295276.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36811..37680)
                     /locus_tag="LTU54_000539"
     CDS             complement(36811..37680)
                     /locus_tag="LTU54_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295277.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37750..39033)
                     /locus_tag="LTU54_000540"
     CDS             complement(37750..39033)
                     /locus_tag="LTU54_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL8295278.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(39047..40732)
                     /locus_tag="LTU54_000541"
     CDS             complement(39047..40732)
                     /locus_tag="LTU54_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBL8295279.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            41007..42032
                     /locus_tag="LTU54_000542"
     CDS             41007..42032
                     /locus_tag="LTU54_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295280.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42627..46301)
                     /gene="rpoC"
                     /locus_tag="LTU54_000543"
     CDS             complement(42627..46301)
                     /gene="rpoC"
                     /locus_tag="LTU54_000543"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBL8295281.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(46359..49976)
                     /gene="rpoB"
                     /locus_tag="LTU54_000544"
     CDS             complement(46359..49976)
                     /gene="rpoB"
                     /locus_tag="LTU54_000544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL8295282.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50704..51672
                     /locus_tag="LTU54_000545"
     CDS             50704..51672
                     /locus_tag="LTU54_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8295283.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51690..52595
                     /locus_tag="LTU54_000546"
     CDS             51690..52595
                     /locus_tag="LTU54_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295284.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52592..53398
                     /locus_tag="LTU54_000547"
     CDS             52592..53398
                     /locus_tag="LTU54_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8295285.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53534..54523
                     /locus_tag="LTU54_000548"
     CDS             53534..54523
                     /locus_tag="LTU54_000548"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBL8295286.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54840..55364)
                     /locus_tag="LTU54_000549"
     CDS             complement(54840..55364)
                     /locus_tag="LTU54_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HBL8295287.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55651..>56801
                     /gene="scm"
                     /locus_tag="LTU54_000550"
     CDS             55651..>56801
                     /gene="scm"
                     /locus_tag="LTU54_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL8295288.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTS"
BASE COUNT        17184 a        11483 c         9676 g        18458 t
ORIGIN      
        1 atgatccgtt attatctaag cactatacag ataataggaa tttttcgttt ctaaatattc
       61 caatttggga atccaataaa aattttcgtt caaatacgtg tctattcgaa ctcttttcta
      121 gttgttaatt atgaaggggg aagaaataaa aaatacaatc tatacacgaa taagaatatt
      181 atccaaaagg aggttttaga aaagatttat tatacgtaaa aagctcaaga cattttctta
      241 gccataagga catgtttgat atttaagaag atatatgaat tttcttttct aagtataaag
      301 ataactatcc attatcaact aaaaaaacat ttttgaaaat aaacatcagc agtcctatca
      361 atgcatgtga gcttatagaa gttattcgca gcgcagcata attgcattag cttcttttcg
      421 tgttttttgc ctcttttccc tttaaatctt aatggaggag gtgtgcaata tgtcagataa
      481 cgacgaacgc gtaaatgccc agttgattcg attctttgaa aaggtcattt tgaacaccgc
      541 taatacttat tataaaaaac aagaagaaat tcaaaaacat gaacaacttg atcaactccc
      601 accatatttg tttactaaag atactataaa atttaaacaa ccgattaatg ttttaaatgt
      661 gactctcctt gttgaagata taaaattagc aaaaatcctt cctttgttga aagaacctga
      721 acaaattttt cttatcgaaa aatttatctt tgataaaact gataaagaaa ttggaaaggt
      781 tctggggatt acaagacagg gtgcgacaaa tttaaaacat cgactttaca aaaaaattca
      841 aaaaatgctg gtttaatatc tgcataataa ctaattttgt atgcttcttt gctcgttctc
      901 ctccttccca aagaaataga ttataagtcc atgggaagaa ggtagattta tgaattcttt
      961 aaaagaagat ttcattttag caaaggctgg aaatgaagaa gctgttgagg caattctcaa
     1021 aagatttagc tctttgattc ataagcaatc ctggagaact ggaaaatatg atcaagattg
     1081 ttatcaagaa tgtatgcttg caatatatct agctatctcg aaattcgaaa ttaaagaata
     1141 actcaactaa gggagtctgt gacataagta aaaagatagt ttcaaatttc gaacaataaa
     1201 tttgaaacta tcttctttat aattataaag tatcgatttt acagcgttct ataatgattc
     1261 atagaatcta aaacgaatga ttttaattat caacgttttt taattcgttt ttagcttaaa
     1321 aataactttt gtcacagtct cactcctaaa attactttat ttacgtatga agtgggcagg
     1381 aggtcgaatc cagaactggg ctatggtact tcaacgagga cttctcctct agaattgaaa
     1441 agcatgcgat ttaccttccg taacgacggt agatttattc aactatgtgt ccaatcaaaa
     1501 tttgtctgat ttcaaaaata gtcaagagaa ttttggttgt taaaaactct tatctatcaa
     1561 cttccacagt ttagttgaca aaccctgata ctaagttggg ctatgataca cttttcttga
     1621 ctatcagtta cgttaccgga aggaatgaac tctattgaga aaaacatatg atcgtaaatt
     1681 taaactaaag atttcacagg atatccttga gaaaaaagta actaccaaga aaattgctga
     1741 agaatacaat atctctcgtc caacaatttc ccgatgggtt tcagaatatc gtcgatacgg
     1801 gaaaaatgct tttgctggac aaggaaaaag attacctgat aaagcagatt tctacattct
     1861 tgaacaggaa aataaacgac taaaagagga agacgagtgt tctacgaaca caaagatcga
     1921 tacggatctc cagggatcac tcaaaaactt tacgatgaag gaatcgaaat caataaacgt
     1981 gttgtagctg ttcttatgcg tgaaatgaat ttatgcacaa gaggatttca tcgacgcaaa
     2041 tcttctcatg gtagagaaaa aacaatcaaa gaaatggtaa aaaaatgttg aatcgataat
     2101 ttgaacagaa ccaaattgat gctgtttggg tgatggatat tacttacatt ccatgtaatg
     2161 acggtcgcct ctatttgtcc acctatattg acttagcaac tcgtattcta cgttactata
     2221 tggtagattc acacatgaaa aaagagattg tcgtccgtcc attacaaatt tattaaggag
     2281 aattgcctac ggtaattcat tccgatagag gtagccagca tcggtctttt atttatcaag
     2341 agctgttgaa cgaccaccac atcacacatt cagtgagcca aactgggact cctgttgaca
     2401 atgcagtaat tgaagcgttt catcgatcaa tcaaatgaga attaatcgac tccaacaaat
     2461 ataaaagtaa aatagagatg aaggtgttaa tccaagatta tttagaagac tactacccaa
     2521 acaaaagaat ccatacaaaa tttatgatga ctccttgaca atttgaagcg aagaaaataa
     2581 gggatctata atatctagtg ttggtgacgc cttctgttgc caatactttt tcttttttct
     2641 aataaacgca aacagacacc ttaaaatcct ttagaataaa gtcgacgaaa accattcaaa
     2701 ggagtgtttc aagatgtcct attcagagtt taccaaagag ttactagata ttctagacct
     2761 aaatcttatc tttcatgaag atgcctttcg caaagagcga atcaatgacg aaacatgttt
     2821 tgtttttgat ggcacattga cctatcaacc ggaggaatgc tttcatcgcc actatcaaaa
     2881 tgaacaaacg atcattaaat agggctggaa aaaagtatcc atcttattga atgatgtcag
     2941 taactacaag accattctgc ggattaacaa acagcgcttc aaatgtaaac actgtggaaa
     3001 gactttttta gcagaagatt cagttagtga tcgtcgctgc tccattgctc gcagagtcaa
     3061 acaagccatt cttgagctat taagcgaacc tctttctatg tctttgatcg cacgtatgaa
     3121 gcacatttct ccgaccactg tcattcgaat acttcgcagc cttcggccta aaaccatttc
     3181 tttgagccag ctgttaccag aagtggtgtg tttcgatgaa ttcaaatcag tgaaaaatgt
     3241 ctcgggagcc atgagctttg tcatgatgga cggcaagacc catcaactga ttgatattgt
     3301 ggaaaatcgg caactcaatc ctttacgcga ctatttcttg cgctacccta taaaagtccg
     3361 tgaacaagtg cgtctagtgg tttcagattt ctatacaccc tatcgcacat tggtgaaaga
     3421 gtgttttccg aatgcccgaa tcgtcgccga tcgctttcat attagccaac acattggtcg
     3481 ggcttttacc aaccaccgaa ttcaagtaat gaagaccttt aaaaaagggg atcgtcggtc
     3541 gaagcactta aagaaatact ggaaactact tcaaaagaat gcgtgggaat tgaaaggtca
     3601 gcatcgctac tggcgtccgt catttcgaga tcatctgaca gaagcagaga tagtcgatcg
     3661 gctattatct tatgatgatt ccttaaagcg aggctacgaa gtctaccagg attttctttc
     3721 cgttatcaga agacaagatg ttcctgaatt cgtcgcgctc ctaaaagaag actacaagga
     3781 gttgccagag cactaccaac cagtgttcac gaccttcaag aaataccaaa cagaaatcaa
     3841 acgagcgttg cgcgttccct attccaacgg ccctatagaa tgtttgaata accatatcaa
     3901 agtactaaaa cgaattgcct atggctatag aaactttcaa aactaccgag aacgaatctt
     3961 tttatatcga ggaaaatatt ttaagaaaac taagaatact acccaactga ctaaagccag
     4021 aacaacaaca cggctagaca agatagctgt ctagccgtgt gagtatctcg attattctat
     4081 tgaatttctt caccaacact cattgacgaa gagccaaaat aagtaatcta accgactgtt
     4141 aactaaacca ttgcaggtcc cgttaaagat acttgtgcct gttgtcaccg cttctttttc
     4201 ttgtccacaa gcgcttaaca cacttaataa agcccctgct attattaata ataatatctt
     4261 tttcttatta aaaacctcct catctatttc cagattagta aatgtagtaa tctgtgcttt
     4321 cttttttctc aatactaata actaacttat ggggtaaaca aatactcgtt tgaccgggtt
     4381 ttgagatcca tccagttcta accgcgattt gatcaggact attatcttct ttaactcgaa
     4441 ttcttccatc tttaatctca acaatattat attgtccctt cgcaggatga taattcacga
     4501 gtttatgatt gatcttatcc aaatcgaagc gatccatttc cttgccatca attcgaacga
     4561 tggcatattt gatagcatct tcagttgttt cattctcctt ttctatagag aatacaaata
     4621 agggaataaa agaggtaata atcaaaatgc caataattat gacatcgcct atcttgacac
     4681 cagcttttct taaaaatttc attaacgata tctggctaaa catattagac tttttccatg
     4741 gaaatggtga aatttttcgg aaattatctg cattaagaga atcagttaaa caaacctcaa
     4801 gttgttcctt aatacattgt tgatccatct tcataccaat aaaaactagt ttattaatgc
     4861 gttcaccaaa caacggatgc caggcttcct tcaattcggg atttttttgt ataatcattt
     4921 gacactcaat tttaggtaaa ctagctaccc aataatttaa tgattcaata cttttatagc
     4981 tacccgatgt ttcaaagatc gtcgaaaaat cactatgttg ggcaaaccaa agaaaccctt
     5041 ttgctcttgt aatattgcca ggaaaatttt tatccgtaag atgggcaaat ttttgtgcgt
     5101 caaggagtaa ccgatttcga tagataaaac ttgagatacc atattcttct gtttcaggaa
     5161 tatgattttg atagccaagc tctaactctt tgaaccagcc cactgaatag gaagtctcat
     5221 ctatattaaa aagatttgta tttagaattt tcgataagtc tacttcacct ttaattgtcc
     5281 taattatttt ggcattggac tgtaacttag caattagacc gttaattttg taaatcgtct
     5341 tggttgacac taagtcacta ttatttaaaa gtaatatgtt acaacactcg aattgatcaa
     5401 ttaacaagcc acgaatatcc tgttcttctt caactacatt accttcagct tttctttcct
     5461 gaatcttttc acccgtttcg aaattatccc aaaaacgttt tacatccact actataataa
     5521 tggcaccaat ctctaatcgt tgggtcaaat caatgtctaa agggttttta gctaatataa
     5581 ttgcttgaat aattggaatt ggtttgctga ttcctgaagc ttcaataaca atttgattca
     5641 agtcagaaaa ttgcactaat tgatctagtt caacaatcaa atcttcgcgt aatgtacaac
     5701 aaatgcatcc attcggcatc gaaacaagtt tttctccagt tcttttaaaa cctttcttgt
     5761 cgactaatac cccatcaaca tttacttccc ctatgtcatt tacaatgaca gcaatttcct
     5821 ttccgtgatc accgtacaga atatgattta gaagtatcgt ttttcctgaa ccaagatagc
     5881 cagttaagat tgtaataggt atttttttca tagggaacct ctttctatac cttgaattta
     5941 cactttaagt gcaacacata aaaatgagtc ataatcgata tccaactagt ataagtttgt
     6001 cgagaacact cctagaagga aaataattat gagccagtat cgtcatctta ctatactcga
     6061 acgagaaaga atctttctgc ttcacgaaga aggattttct attagtataa ttggcaacga
     6121 cattcagcat agcccgttta ctattttaca tgaactgaga agaaacaaga aaaaaactat
     6181 tcaccttcca aagcggaaaa aaattatgct tatcgaagaa aaaaatgcgg acgttatcac
     6241 attctaaatg atccgtgagt ttggtcaatt gttcgtcatt tatttgtcga ttttcagtgg
     6301 tcgccagagg aaatctcgaa tcgtttgaag catgaacata tggacttttc aattagtttt
     6361 atcacgatct aacgtgcaat ttgcaatgat cttttcgata cagaacccct ctcacacggc
     6421 aataaaggcc tgatccgttc attgcaccat cgaggaaaaa caagacatac aaaaaatcat
     6481 gtcgagtctc gtggaaagat ttgccttact cattcgatcc atggccgtcc aaaatccgca
     6541 gatgatagaa gtgattttgg tcattggaaa gccgatattg tagctggcaa aactggtggt
     6601 acttgtttag tgactttgac agacagaaaa tcatgcttcc ttttagcagg aaagaaggtc
     6661 cccagaaaga aagccgctta tgtgagcgag aaaatgattg aacttttaag cagtattcca
     6721 aatgaggcgg tcaaaacgat cactccagat agaggaaaag aattctccaa tcatttcgca
     6781 gttacagaag cattgaataa tatgcccttt tattttccag atcctcatgc ccttggcaac
     6841 aatgaacaaa ttaaaatacg aatagactca ttcgagaata cttgccaaaa tcgaaagaga
     6901 tagctacggt agataactca acgataaatc agatgattga aaaactgaat aacagaccta
     6961 gaaagtgttt agattggaga acaccttatg aagtcttctt tgataaagtg ttgcgcttga
     7021 tttgacaatt caagacataa taaaaagcag atcaaatcca aataagggaa aaatgatcta
     7081 ctctggtatt atcaattaat tggccaagct attatgccgt tttgctaaac gtctacttct
     7141 tccagtattg accttgataa aacaacaatc aggatcccgt tttaatactc tatgaactgt
     7201 gtcaatacta acatgtaaag ttgcagcaat atcagctaat gtactcccat ttaatctcat
     7261 ttgctttgca aatcgataac gccgataagc atcaatattc ttggaatcca tagacaataa
     7321 tacttttctt accatgtatt tcctccaaaa attgaatttt aatgagccat catttcttga
     7381 gcaatttcat gtttagacaa tgaagctgga tagaaagtag gccaattatg caattcatta
     7441 tagagttttt cttgactttc tccaccaaag aaaatatgga aatgctctgc cttacttggt
     7501 gcaattccat gatcactaaa ttgaatatat ttaaaacgac cagcacctgc atcatcagtt
     7561 tcaaaattaa aacgaacacc acggtttcct ttttcataat tcaaaatttt gtatccaaca
     7621 tatttgtatg tgtattgatg atgttcaccg ttgactacaa aatcaatggt gttgtcttta
     7681 atgttgatag tatcaatatc tgtcttatag ccagtagtat aataatcttt gtactcggca
     7741 gctgtcatgt ctttattgat ttttgctttg taatcgaaaa cttcatccaa aattccatct
     7801 tttaacaacg gataaacaga ttgccattcg cctgtataat cactcaattc gcgatccttc
     7861 acatcggcat ctttaaagta cccattagca actgtctttt catccttcgc ttctttttct
     7921 ggaactacct cttcaccagc aaccatagtt gttttttcta acgcttttag attgtcttcc
     7981 attactgaaa cataatcttc accattatcc atttgttctt ttgtaagact ttcaagagga
     8041 ttcagtactt ccaattttat cccagcttcg ttggccaaag tttttgcaat tttatcatta
     8101 gcattctttt caaagtaaat ataattgatc ttattttttt taacatactc ttttaattca
     8161 gctaatcgac cagaagatgg ctcttcttca ggacttagtc cagcaatagg aacttgtttc
     8221 aatccataat ccaaagctaa atatccaaag gctgcgtgtt gcgtcacaaa acttttttgt
     8281 ttcgcttctt taagactagt ggtatagtca gcatccaacc gtttcagttt agtcaaatac
     8341 ttttcagcat ttgtctcaaa tacttttgct tttttcggat acaatttaac taactgatcc
     8401 ttgatatttg aaacttcctt aattgccatt ttgggtgaaa cccacgtatg aggatctagt
     8461 tcatgatggt gatcttcttc tccgtgatca tgatcttctt cttcactacc tggcaataaa
     8521 atcatgtctt ttgttccttc tactacatta ggcccagctt tcttccaact ttctttagct
     8581 tttggtaccc atgattccat gttttcgtta tgatatacaa aaacatcagc atcatgagct
     8641 tttgccatat ccttagctga gggctcatag tcatgaggtt ctgatcccgc tggaaccatt
     8701 aaatccacgt tcgcttcatc gccgacgatg tttttggtga aatcatacat tggatagaat
     8761 gttgttacaa tttctaattt tccatcttta tcattcgata cttttgcatc attagtgctg
     8821 gagcaagctc ccaataatcc tagagctgct atcccaataa gtcctacact gattttcttc
     8881 attctttttc cttccttaat tatgctaatt tcttcatttt atttcgttta tgttctttga
     8941 taatttttaa tgcccttttt ctagttttaa tattcgggct tttaagtcgt cgatatgcac
     9001 tagatatatc cgttttctcc attagaaatt acccccaatc actacttatc attatttgct
     9061 ttccgccagt aatttatcaa aactctgttt gatcctttct tcatccaaca tttttccgat
     9121 tagaataata gtggtttggt ctttttgtcg agtggtgtat tgaaaattaa cctgttcgtt
     9181 tacctcttgc atggcaacta taaaatcctg ctcttgtaaa gcaatcaatc ctttaaaacg
     9241 atacaggctc ccttggttgg ttaaaatcag ccagtcaatc cattggagta aaaaatcttt
     9301 ttttatgtct tgatctgaag ttaagagaat tgatttaaac tcatgtactt gatggtgttg
     9361 atgatagtat ttgccattat agtgattttc tgatgaatca ctttcaaaaa ctaagggatg
     9421 attaaatttt ttcaaattat aaaaatcctt tttttgaatc gtttcactat gtgtgaacat
     9481 tcgaatatcg gataaagggt taatactttt gatttctctc tgtaattttt ttagtgcaga
     9541 taaattcaag ttttgttttc tagacacaaa aatgcgatct gccatgacaa gttgcttaag
     9601 ggcttgtggt tctctttcaa taagttcaaa actgtcacta tcaataacag tcaataaact
     9661 atcgatatat actcttcctc ttagttgagg cgctgttaaa agtgtttgaa tgattggttg
     9721 gggatctgct atacctgtag tttcaatgat gatttgtgaa attggttgtt gatgttcttc
     9781 tattgtttcc agtaaagaaa tcaatattgc taataaatca ctgcgaagac tgcaacacat
     9841 acatccatta tttagttgta acacccgttc ctcgctatga actaataatt tgtgatcaat
     9901 cccaacttca ccaaactcat tttcaattat aataatttct tccctggggt agggtgagac
     9961 ttgcaaggct tgattgatta gcgttgtttt tccagcccct aaaaacccag aaacgattgt
    10021 aactggtatg cccattttgt caccttttta ccacttagct atttcacttc tttaaaaatt
    10081 gcccgtctgc gcaattttgg cgaatacttt tttaattcta accgttcagg gttattccgt
    10141 ttatttttgc tggtgagata cagacgttct ccagtttcga cacattccaa aataatattt
    10201 tgacgcataa atcttcctcc ttcaatactt atcgatcttc aagatatgct tgaatttgtt
    10261 gtgcatcttc aaaagttaac tcgccagatt ccaattgtga aactaaattt tctttcatat
    10321 caacaggtat ctcccaataa ccatcgatac attgaaataa ctcctcgtca tactcttcaa
    10381 aaattggata gattcttaaa aaatcatcaa atgataagcc ttggtcctca tagtaatctt
    10441 tgactaatcc attttcaatt gcctgttggg tcggtaaacc cgtttcagta ataattcctc
    10501 tctctaccat cgaacgataa aaagtgcatt tatctagcga ttgctgggtc gtttcaagat
    10561 aatactttaa tccttcttca actaaaggtg ggtaaataat ttccattttg attccctcct
    10621 aaaagttata tattaaacta ttttgaagct cctttaatta ctttctttcc attatagtac
    10681 ccttttaaag atacatgatg gctacgtcta taatcaccat tcgcttcgtc aaatgaaatt
    10741 tctggtttta ctacagtttg atgagttctt cttaaccgtt tcttcgcctt agatgttttt
    10801 cttgctggta ctgccatgat atttcctcct tttaccaact tgcttttttt actccaggaa
    10861 tcaacccttg atgggcaagt tctctaaact taatacggga cataccgaac tttcgcatat
    10921 accctcttgg acgaccatcc gtctgatcac gaagcctcag acgattcgga tttgaatctc
    10981 ttggcaattt agcgagagcg gtgcgatctc ctgcggcttt taattcctga cgtttatctg
    11041 catacttttc aatcatcgcc atttgtttct ttgctttagc aattttcgac ttttttgcca
    11101 tataccttta tactcctttt attttgattt agacacctca aatcgtaatc gttacgtttt
    11161 gacactgata ctttaccaac aagagttgct cttgtcaaca ataaataaca tgaaataaaa
    11221 ctcatatgaa tagataacta tttgaaaacg aatttatcgg tacatagaga taatcaaatc
    11281 atttaaaaga atatgcagtt taggctctga aaaataagaa ttcactttta ttagtccaat
    11341 caaacacaaa taccgtttta agtagctttt cctctactta aaacggcaat atgaaagaaa
    11401 ctataacttt ttattcaatc ttttatgttt aactggttta tcgccaattt tttaaaactt
    11461 tgattattcc ttatattata agaatagaca ttaagtgaac taattcaatc atactgattc
    11521 tttcgtgtat tcaaaattag atgctgttta ataaaattat tctttttgaa aaagaattat
    11581 aactatatcc caaaaattgt aggttaattt aaagcctatt tcatcatcgt gattgagctt
    11641 ctttttcata gacgaatttc tcagatagtt tagttttctt tatgagattt ttgtatattt
    11701 ctgaccttcg atacaactca ttatgagtac cttgatcaac tagttttcct tgatccatca
    11761 ctacaatttt atttaccttt tcaatagact tcaagcgatg cgatataatt agtactgtct
    11821 tatcttgtat taaacgattt aaactttcct gaatacgttt ttcattttcc gcatctaaac
    11881 tggcagtaat ttcatctaaa attaaaattg gcgcattttt caaaaaagcc cgtgctatgg
    11941 ataggcgttg tcgctctcct ccagaaagtg aacttccgtt ttctccgacc aatgtatgat
    12001 aaccgtcagg tagtttttca ataaagtctt cgcagttagc taaacgagct gcttttttca
    12061 cttcctcatc tgacgcttga ctatttccaa tacgtatatt ttccaaaata cttgtattaa
    12121 acaaagttac ctcttgaaaa acaatagcga ttttgctaaa caaagaagct ggagatacac
    12181 gtttaatatc atacccatca attaaaatac agccttcatc ataatcatac agtctagaca
    12241 ctaatttcaa gatactagtt ttcccggaac cactagcacc tactaacgca gtcacttctc
    12301 cttgtttagc cgtaaaagag atatgatcta aaattggggt attgttatca tatgagaaag
    12361 agacatctcg caactcaatg tcaaaagatt tcagaggact atctgatccc tcttggatac
    12421 tggtttcctt catagctcta attcgctgta ttttaggagc taaataaaat atttcaagca
    12481 ctgcttcttt catagaatcg aaggaatcct ttatttttat tgcggctagt aagtaaccaa
    12541 cgacgtagag tatagtcact tcacccgtta acagtaagtg aacacctact agaatgacta
    12601 ctgctaaact aacatatgag aaaatagagg ataaggccat gactgaaaaa gtacttaatt
    12661 ctacctttaa atgaatcctt tcactctctt ccattttttt gaaaagtctg tcttgaactt
    12721 ttttagatag attaaagcta ttaatctctt gctgcaattc tatagtttct tgaaattctt
    12781 ccgagttttc tctcaaagta tcgtaatatt tagtattggc tttcgtttgg gatttctttg
    12841 ataacaagat taagacaaaa ctaaataacg ttggcaaaat aacagctaat cccattttga
    12901 cattaccaac caaaagcatc actgaaataa aagggaaaaa cagagccata ccgccggctt
    12961 taggtattgc atgactcatc gcatgctcaa taccttcaac gtcagacatg atagtttgag
    13021 ataaatctga taaattatgt tttgaaaaat aggataatgg taaatttgac aaatcgtctg
    13081 caatttgcac tcttaaatgc gcactttctt tataagtact attatataag ttttcatact
    13141 ccctatctaa caaccaatac atgaaaagca aagtaacaaa cgatacggca aaataaagcc
    13201 aacggggctt tgtattttct aaaactaatt cgtctatcag catcattata atgaatgctg
    13261 gtaccatatt aatgcagtat acaaaaaaac tagaaataga agctttagtt aaagcttctg
    13321 cgccctgatc acttaaagca aatttgtttt tataaaaact tttcattgtc aagcctccaa
    13381 tcatttgcat tctcataatt agtgaccaat tgtttataca cttgatgttt agccattaat
    13441 tcattatgat ttccacgttc tactattttc ccttctgaca agacaagaat ttcatctaca
    13501 gatttaatgc tggataatcg atgagctatc ataataactg ttttttcttt tatcagatgt
    13561 tgaaaagcct tttgtagttc atactcatta tctggatcaa tagatgcact ggcttcatcc
    13621 ataataacaa tctttgcatc ttttaaaatt gctctggcaa tagctatacg ttgtttttct
    13681 cctcctgaca gataaactcc tttactacca attaaggtat gctctctttc gggaaatttt
    13741 tctaaaatcg attcgcaacc ggctaatctt agcgcgtcaa atactttact tgctggcgct
    13801 tgaggatttg ctatagaaac gttatcgtaa atactctgat tgaatagttt cgtatcttga
    13861 aagacaaaag caattgtgtt aataattgtg tcttttgaat agtgatttaa aggcttacca
    13921 ccaattttaa tctgtccctt atccacattg tagaaacctg aaattagttt agctatcgta
    13981 ctctttccgc ttcctgattt tcctactaat gcataaattc ttttttgttc taaaactaag
    14041 ttgaaatttt ctaaaacaca ctcttctttg tatgaaaagc tgacattttc aaatgcaatg
    14101 tcacaatttt taatctgtgt ctcgttccca tattttagtt ggtcttgctt catttctaaa
    14161 taaagggagt ctagtgtttc gactgcatat cttgcttcaa acaagtgcat acttacatac
    14221 attattctca tgaaagaaac aaaaataatt ccacttaaaa aaaacaacat caacaagtca
    14281 acagctaata tcttgggagt ggtaagagaa gttataaaaa aggttatagg aactatcaaa
    14341 atgcaaacta tgccaaaaaa tagccattga aaaagaacaa aaggtgtttt cccactcaaa
    14401 gaatacttat atgcaaactt agcataatcg ttaattgctt tattcaaggc tttaaatgat
    14461 gttactttag caccaaatat ctttataact tgaattccgc ggatatattc taccgtttca
    14521 gagcttaaca catctaacga ttcttgatat ttttgaataa aatttgtatt ctctgtcatt
    14581 ttctttaaca agaatacacc aattactaac atggcgatta gaaccaatcc aattttaaag
    14641 ttaatgaaaa agcctaaggc aactgataaa acaggtgtta ctatggcttg agcactatct
    14701 ggaatcaagt gagcaattgc catatgtgtt ttagccgcgt tgtcatcaat tgtctttcga
    14761 atatatcccg aagaatttaa atcaaaaaaa cgaaaacttg cttctgttaa accgctaatt
    14821 cccttttttc tcaaattagt ctctaagcga aacccaagga tatgagaaaa taagccagaa
    14881 aaaatataaa ataaagctcc taaagttaac caagcaacag tttgaaccgc ataactctta
    14941 gctaaatatt catctcctac aataattaga gactgaagaa atttgtaaat actgtaatat
    15001 ccatagactg ttaatacgac agaaaaatca gaaaataaag tggctaacca tccgtagtat
    15061 ttcattttag ggacataact aaataatttt ttgtatactt tcattttttc ctcccccgca
    15121 ttcaatttct tctcttattc gtttacaact gttaatgctt tccgtaatag cgtaaattag
    15181 agatgtacaa acagataaaa ggtaaactac gttctatttt ttgtattttt ttaagacttt
    15241 tcttccaatc aatacaccaa tcatagataa acttgctaca actaacactg aaatgattcc
    15301 ccatatacct accgcatagt ggatcattgc atccattgtt gcagcagatt gtcctttatc
    15361 tatccaatct tgacgaaaac gatctgtaaa gaaccatata ggaatccact gtccggcagc
    15421 actgcctaat tgcattagag cccaagacgc aacttgagga attgctcgat catatccaaa
    15481 tacagataga ataatatcag ctaagatcgc catactaatc gaactgataa accaaggtaa
    15541 gtacgccccg cccattaacg taaaaaataa caataaaaca attgtgtaaa tagtaaaaat
    15601 tccttttcta gggtagttat agctaatata aacaaatatg attccagata ataatccaaa
    15661 aatcccgggg gatatgctgt gaccaaacgc cccaccaata ctactaacca ttattgaaaa
    15721 aaagtaaatt actagggaaa taactgtagc taaaccaata aaaatataat cttttgtttt
    15781 catatatatt ctcctcaatt ctatttcgtt atgtcattaa aaatctgttc tatcaaagtt
    15841 gaatcttcta ctctaagttt tatatctttt tttattccac cattttttaa ataaataatt
    15901 ctattgcatg ttttataaag aaattccgga tcatgagtag ctataactac tgcacccttt
    15961 tcttttaagt ctttaataag gtcagcaatc ctttccatat taatataatc caacccactt
    16021 gtaggttcat caaatagaaa taaattagtt tttgacaatg aagctataga aaccaataat
    16081 ctttgttttt ctccaccgga taaatcaaac ggtaattgat ctttcatttt ataaagttcc
    16141 atttttttta aaattttttc ttgctgatct ttactaacta tttcattccc cagggctaac
    16201 tcgctcctca ctgattctgt gaaaaattgg taatccattt cttgcattac taaaaacggc
    16261 gatgacaaat tttttttatt ttcgattatc tttcctttgg tagggcgaat aatcttcatg
    16321 agtattctta gtaaggttgt tttccccaca ccgtttttcc ctaccaaacc aataacatct
    16381 ccaactttga catcaaaatt tatgtctttt agaatatttt cataagtaat ttcttcaact
    16441 tttaatacat ctgcacttac cttttcgata tagggaactt caatcgaact attaaaaatg
    16501 tcaaatttcc ttaatgcgtt attgttttca gtttttaaca actgaatagt tgtagttttt
    16561 acagttctat ttttaacaaa tagaacttga tcaattaatt ctttcaaata gtaaaaacga
    16621 tgttcagcta taacaatagt aaatccttta ttttttaaat cacaaagtaa gtcagcaagt
    16681 ttcttagtcg ctttgaagtc caaatttgaa gacggttcat ccaatataat taatttagag
    16741 tttagcgata aactacatcc aatagaaatt ttctgccgtt caccacttga tagcgaaaaa
    16801 atgtttttat ctaatatatt tgaaagatct aataattcag ctaattcatt gattctttta
    16861 tccatatgct cttttgtaaa tccaaagttt tccatcgaaa atactaactc actcgttgta
    16921 tttgttgtaa agaattgtcc tctaggatct tgacttacgt agccaatatt ttttgatata
    16981 ctttcttgtg ttaaggattg tagcgagtta ttttcaaata aaatttcccc aaccaatttt
    17041 ccgtcataaa gtcttggaat aagtccattc attaacttta atagtgtgga tttcccactc
    17101 ccagaagaac cagtaatcaa tgtaagactg ccttttttta ttgaaaaatt aaggtcaaca
    17161 agcgcttgtt ccttaacatc cttatatgaa aaagaaacat tgttaaattc aaacaattct
    17221 ttcacctcaa atcaaaatac ttaagaaaaa aatagtaacg gaaaacagga tgatatagtc
    17281 taataagctc atgtttgttt tatagtaaga acttcttttc acaggagatt ctatcccctt
    17341 tgttattgca ttagcggtca tttcttcagc aatcatagtt gcacgtataa gaacgggaac
    17401 taacagaaat tccaaatatt gcagaggctg aaaaaaagac aagcgaatgt ttctcatttt
    17461 taaagattct ttaatcattc ttatttcaga acgataagtc ggaaagaatc tgatcactac
    17521 tgtaatactt aacattattt ttttcggtaa atgtattttt tctaaagagg ataataattc
    17581 attgctagta tagacactaa ccaacaacca tgagatcatt acgaccggca acattcgaaa
    17641 tacaatatat aaggatgtcg caaaaaatat agtaaaaaac tctatcttga tagcatcaat
    17701 aattttcatt actaaaaaca gaactgaata aatcaagatg aaataaatac atgtctttat
    17761 tttacgaaac atcaatacta aaactgtagc aataatgaaa caccccactt gaaatatatt
    17821 tccctttcct aaaaaaataa tgaccggaaa aaaaatattt aaagtaatca aggttaacgg
    17881 attgattttt gacactcttt cacctccaaa aaataagatc gcactgttat cttaagctga
    17941 taaaaatgca ccgttattca gataacagtg cgatcttatc aaaaagcata actgagaaca
    18001 attctcaatg caagcttttt ttaattaact cagtaattca atccacccag gaacagaaaa
    18061 ttgatacaat cttctggtta atagcatagt cttttctttt gaatagctat gcttcaatat
    18121 atcatagaat gtagcgtaga atgagtaaat gtagaagtgg atttcttctt cattaaagac
    18181 taaattttga ttgatcattc cgatagtctt taacttatct agacaacaga ctgtcttttt
    18241 cgtcacaaat gatgtaattt tttcaacaat atcttgatat ttggttccag ctgattggaa
    18301 taaaatcaaa tcgaatatac gtttattctc aaaaatataa ttaataaatg gatctaattc
    18361 attgaagttc tctctccaac ttttctctat atctatattt tctaagttta agaggtattt
    18421 ggtaaactct tcattataca tattaataag cgcattaata tgcggctgaa taatttcttc
    18481 aaataacgct tccttagaag taaaatgttt ataaaaagca cccgtagtta cgcctgcggt
    18541 caggcacagt tgccttatct tagtttgctg aaatccttct ttttcaaaat ggcttagtcc
    18601 actttctatt aacttttggt gagttataat ataatctctg gtttctatgg gtacttcctc
    18661 ctaataagat gaattaactt tattatctta cacaggactt gaagtttcaa tcgttaacgt
    18721 ttattactga actcattcct tagagctaaa ctaatgagaa agagcagaat aactgataca
    18781 aaaagcaatc ctacaagaat taatagactc atctaaatca ttcacctttt ctaaaataat
    18841 ccgagaaaga tttaaaaaaa ttatatccag ctattaggat aagtcctatc ataatcactc
    18901 caatagccat tttcttatcc tccctaaaaa taaagtcttt tacttaaaac cttttttgta
    18961 atcgtcaata aattttttcc ctttatcagt taattgaaat cctatatcta aatagtcctc
    19021 atatctaagt ttagccagca tctcacttac agatgctgga ctaacactta gaacttttgc
    19081 taaatcatta ttttttaatt tcttttttct ttttgataat tcctgaattg ctagtaaata
    19141 aattagtata gtagtcgttt taatttgtac catacctacc ctccaaatta tttcgaaagg
    19201 ccttcagcaa ttttatccag gttccatttc atcatcgcat agtaactatc accatcttgt
    19261 cctttttctg caattgaatc agtaaaaatc gttgaataga taggaatatt ggtatctttt
    19321 gatactgttt tcattggtct atcatccaca ctgctttcta cgaataagga gggaactttt
    19381 gttgtgcgta atttttcaac taagtgtttt atttgatctg gtgttccttc ttcttcagtg
    19441 ttgatttccc aaatgtaagc agaaggcaca ttatacgctt tcgagaaata tttaaagcat
    19501 ccttcacttg taacaatcat ttttttatca ttcggaattg aagcaaattt agatttagct
    19561 tctttgtcta gagaatccaa tttttcaata tacttatcta gattttcttt atagaatttt
    19621 ttattatcag gatctttttc cgctaactgt ttttcaatat ttttagcgta aataataccg
    19681 ttttctaaat ttaaccaagc atgaggatct tccttccctt tctcactctg accctctaag
    19741 taaataacat ctatgccatc acttgctgca aaatagtctt tgttttcctc tttgttcgca
    19801 tttttaacta attttgtaaa ccaagcattt cctccagttt ccaagttaac accgttataa
    19861 aaaatcaaat ctgcttttga agttttttga acatcttcag gcaaaggttc atattcgtgg
    19921 ggatctttcc caataggtac gatactgtgt aaatcaattt tatcttttgc tatattttcg
    19981 gtaatatctg ctaaaatcga attagtaact actactttta atttttcgtt cgaagccact
    20041 ttatctttgt ctttactatt tattgaacat gccccgaata taaaaagagc aatcaaaatc
    20101 cccagacttc ccagtactgc tttccatttt ctcatcataa cgatctctcc ttctttctta
    20161 aaaatttttg ttttggtgag attagaaaac taattacaaa taagaatgcc gctgttaaaa
    20221 caatacttga tcctgctgct atgttaaaac tataaccaat aaacaatcca agcaatgatg
    20281 aacttgctcc gactgttgca gaaataaata tcatagtttt caagctattt gcatataaat
    20341 atgctgtagc tgctggtgta attaacattg caacaattaa tatagttccc acactttgca
    20401 tagctgttac tgctactaaa gttaatagaa ccatcaatag ataatgataa aaactgacat
    20461 tcatccctat tgcttttgcc attaatggat caaaagaagt gattaataat tctttaaaaa
    20521 atagaactac tataaccaac actaaaaccg aaacgccaat tgtaatccac ttatcaatat
    20581 cttgtacagc taaaatattt ccaaataaaa tatgaaataa atccgttgag ctatttgcaa
    20641 ctcctataag gataactcct agtgctaaaa aagaactgaa agtaatacca attgctgtat
    20701 ctccttttat tacactattg ctttttataa aagtaataat gatagaagct aatagaccaa
    20761 atacaatcgc accaataaaa aaattaatac ctaaaatata agaaattgct actcctggca
    20821 aaacagcatg agaaatagca tcacccataa gagacattcc tcttaaaata ataaaacaac
    20881 ctactgttcc agaaacaatc cctataacta cagatgtaat tagagcattc tgtaaaaagt
    20941 gaaagtcata cagaccatta ataaaatttt gaagcatatt attccccctc tccaataaat
    21001 atagtatctc cataggcttt ttttaaattt tcttcattaa atacagattc tacacttccg
    21061 tgagcaatta gatttcgatt caaaagaata atattatcaa aatacttctt taccttgctt
    21121 aaatcatgat gcacaattag cactgttttt ccctgatgtt tcaatttttt taacgtatcc
    21181 ataattattc gttcactaac taagtctatc cctacaaatg gttcatctaa aaatatgaaa
    21241 tccgcatttt gagcaagaca tcttgctaaa agtacacgtt gaaattgtcc tccagataat
    21301 tctcctattt gatgattaga atattttccc atatcgacct tttccaaagc ctttgaaact
    21361 ctttgccatt ctgcattttt aattctttga aaaactttca ttccggcata tgttcccata
    21421 gatacacatt cttttacttt tattggaaaa gtaaaatcaa tatcagcttt ttgctctaca
    21481 tatgcaatct tcaccagttt tttattgata tcttcattat taatggagac ctctccctga
    21541 tgagggataa tatttaacat tgctttaagc aatgtggatt ttccagcacc atttggacca
    21601 ataatacctg ttaaagcacc ttcttgtatt tttaaagaaa tctcgttcaa tgctaaaaaa
    21661 tcgttataac aaacattaag ctttttcact tctaacataa ctttctcctt ctaaatgata
    21721 atgattatca cgatcatatg gtatacaaaa attatatttt agtcaaccct aaattttata
    21781 gtttagattt ttttctgttg ctgaagatta gaatgacaaa actatttgaa actacgcgta
    21841 tagtaagtag ctttcaaaat atataaaaaa ggagcgttct caaatgagaa catgctccta
    21901 aaactacttt attggcgtat ggaaaaatta tacttgaagt ggatagcggg ttgaataagt
    21961 tgataagcat caaaacattt ataaaaatta ttcaagacta tgtctgttcc atatacaaat
    22021 atgaaaatca gtaaactcca tttaactttt ttcgtagtgt gtctttttat acatgtttat
    22081 cgttattaat agtgaatagg aaaaaaacta tttttgatca ttgacaatta aatacaattt
    22141 ttgaataacg atccctgcta ttcgagctag aaactcaagt acgccatgac ctgattagcg
    22201 acaaatactt agagatttct ttttgttctg ggaaaacaaa atgccatgac aatagcagca
    22261 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
    22321 tacccaaagc aattcaaaaa tttcataact actattttag gagatgattt tatagaatcc
    22381 agagtctacc tgaataaaaa acaaacctgt gcatttctag gaatcgataa taatactcta
    22441 gataagtgga ttcgtatata taatttccca gtaataagag ttggtggtgt agttcgcttt
    22501 gacgtagaag atattaaaga ctttatgaat acatttaaaa taacttgaaa ttttcttctc
    22561 gccgcatata ctaaaattag tgaaatatac tgccttactt tttttaagga gtgaggattt
    22621 tgcctacaaa gctttcaaat gggaaatata aaactaatct gagatatcca aaaaggttta
    22681 aagaaataac aggaattgca tcagaaaagt atcaaaaaac atttccaaat aggcaattgg
    22741 cgattaaagc cgaaaatgat atgaagaaga aaatagaaaa agttttgaga gaggagaatg
    22801 caaactcatt agaattaaaa ggaaaaataa cgtttaaaaa attttacgaa tctaaatggc
    22861 ttcctagata tgaattaggt caaacaatta gatcaaacag gcctcctagt gatataacca
    22921 ttagtaatac aaaagatata tttcggttgc atattttacc tatgtttggt gaatacgcaa
    22981 tgaattatct aaatatcaat actgagataa tatcagatga attaaccaaa aaatcgaaag
    23041 aatatgcaaa tataaaaatc ataaaaggat atgttcgttc catgttcgat attgcagaaa
    23101 tactaaatta tatcgaattt aatcgaacta ctaaaattat tcaaagtatc actgcaccta
    23161 aaaaaaatgc tttagaagaa aaaagaatac aagaaggtaa gcaagcacta tcatcaaaag
    23221 aacttactaa ttggatagaa gcagtaaatg atgattttaa taatcattta cttacttttc
    23281 atgattacac actttttatg attaccctgt atcttggtga tagaaaaagt gagacttacg
    23341 ctttgcaatg gaaatatatt gattttgaaa aacaaacggt ccgtcttaaa catactttag
    23401 ataaatatca aagaaaaaag tttacaaaag gtcgaaaaga taccgtcatt caagtaccag
    23461 aagttgtaat gacattgcta agtgaatgga aatcggtaca agctgatcaa ttgttgaaat
    23521 taaaaattaa gcaaacgtta gatcagtatc tctttactta tactaaacca tctggagaag
    23581 taaattgtcc tgttcatgcc gattacctaa actatcgaat caattcaatt aaaagaaggc
    23641 atcctgactt agttcattta tctccacaca aactccgtca tacttatgca actatagcta
    23701 gacaaggtgg agcagatatg aatcaaattt caaatgcttt aacacactca gatatttcta
    23761 caacaaagat atatgtgaac acaccagata tagttgataa ggctgttttt gaagcttttc
    23821 agagaggatt gaataagtgt gattgatatg tgttcttaaa tgtgttctta aaaacaaaag
    23881 aagcactttc caaataattg aaaagtgcca gaaacgttga tatataaagg atattaacgt
    23941 tttgagaatt gtgaagcttt acgtgctttc ttaagacctg gttttttacg ttcgaccata
    24001 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
    24061 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
    24121 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
    24181 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
    24241 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
    24301 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
    24361 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
    24421 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
    24481 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
    24541 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
    24601 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
    24661 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
    24721 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
    24781 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
    24841 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
    24901 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
    24961 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
    25021 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    25081 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
    25141 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
    25201 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    25261 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
    25321 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
    25381 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
    25441 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
    25501 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
    25561 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
    25621 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
    25681 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
    25741 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
    25801 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
    25861 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
    25921 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
    25981 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
    26041 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
    26101 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
    26161 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
    26221 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
    26281 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
    26341 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
    26401 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
    26461 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
    26521 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
    26581 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
    26641 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
    26701 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
    26761 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
    26821 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
    26881 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
    26941 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
    27001 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
    27061 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
    27121 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
    27181 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
    27241 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
    27301 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
    27361 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
    27421 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
    27481 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
    27541 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
    27601 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
    27661 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
    27721 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
    27781 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
    27841 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
    27901 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
    27961 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
    28021 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
    28081 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
    28141 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
    28201 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
    28261 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
    28321 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
    28381 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
    28441 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
    28501 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
    28561 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
    28621 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
    28681 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
    28741 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
    28801 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
    28861 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
    28921 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
    28981 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
    29041 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
    29101 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
    29161 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
    29221 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
    29281 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
    29341 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
    29401 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
    29461 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
    29521 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
    29581 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
    29641 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
    29701 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
    29761 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
    29821 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
    29881 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
    29941 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt ttatcgaact
    30001 ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca aatgctaaca
    30061 gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact tgaggggcaa
    30121 atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt tctttcaagc
    30181 ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga ttagtaacat
    30241 cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt ttatcaactg
    30301 tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct tgatcgtaaa
    30361 aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta cttccgccaa
    30421 cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt tctcctactc
    30481 ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc aatccgacga
    30541 tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt tctttgtttt
    30601 tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg tctgctaatg
    30661 atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca gcctgaacaa
    30721 aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc agactttctt
    30781 tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca agatcagttc
    30841 tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt tcgtctcttt
    30901 ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt tccaaataaa
    30961 aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa gagttccatt
    31021 cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc ctaatgaagc
    31081 agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca ggcacgcttc
    31141 ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta tggcgtgaat
    31201 cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca aattcttaga
    31261 aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa gatctgcttg
    31321 cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa tagactggct
    31381 gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat acgtgtcatc
    31441 ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta aaaaattttg
    31501 acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg ccacattata
    31561 atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt catctggacc
    31621 aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca gccaaaaagc
    31681 tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat cctctgttgc
    31741 ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt ctgcggcagc
    31801 tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt tgggtgtctc
    31861 ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt gataatctct
    31921 tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat tctttgctac
    31981 ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc agaaaacatg
    32041 ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg gatcattgcg
    32101 gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat cgaaagaaga
    32161 atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag acatcgactg
    32221 ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt tcttaaacgg
    32281 gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa aggacgtatt
    32341 ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt ggaatacacc
    32401 tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg tctggtagga
    32461 tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact gctgtgtacc
    32521 aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt cttcggtcaa
    32581 cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag caacacgaca
    32641 agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc gtttaagctc
    32701 tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct caagagaaaa
    32761 atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca ctaactcggc
    32821 tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc ttacttgatt
    32881 ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa tggtcatctg
    32941 actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt tcataaggta
    33001 ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg gatgacatat
    33061 ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt cacaaaatga
    33121 gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga aaccagcttt
    33181 ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg atcgccacaa
    33241 aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt cagttggatg
    33301 atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt gaatttgctt
    33361 ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg gaagctgtac
    33421 gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc aagcatgtga
    33481 atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa cgttccccag
    33541 cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc cccgagaaca
    33601 tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa tccataagcc
    33661 aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc agctgtcata
    33721 gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa tacatgttcg
    33781 aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc attcagcatt
    33841 tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc ttctgctcgc
    33901 tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt ttgataataa
    33961 gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt cttcatttat
    34021 ttctccctcc tttgcttatt tacttattgt tattctttct ctattctttc tctaaatcat
    34081 ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc tgataaattt
    34141 tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca tactcatttt
    34201 gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt tggtagcgat
    34261 gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat gcttttgaaa
    34321 tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca tgtttcaatt
    34381 gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg tcttggtatc
    34441 ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta tctaaataac
    34501 gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa accatcggcg
    34561 gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg cctccaacct
    34621 cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc cgttcttcga
    34681 taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg atccctgttt
    34741 ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg tcgatttcgt
    34801 attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct cttgggaaaa
    34861 atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa cgcccatttt
    34921 gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc tttctgcaaa
    34981 tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca ataattccca
    35041 acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg gttcactaga
    35101 tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa gattaaaccc
    35161 agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc cgatccctct
    35221 taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat ctactccacg
    35281 tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat ctagcgtgta
    35341 atcggcaccg tatttttctg ctaattctct acgctgcgct atgggatcga tcccaacgat
    35401 ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag cgcctaaacc
    35461 gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac ctaaagcgaa
    35521 ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa gtttacgtag
    35581 tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat aagtgcagac
    35641 taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg taccgacaat
    35701 catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt cttctcttgg
    35761 caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta atcctctaaa
    35821 gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac gtacttcgtt
    35881 cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt ctaatgcaac
    35941 taattttttc atcttttctt tcctctttct tttcttttta tccttttacg ccgccttcga
    36001 ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact ggtaaagcgg
    36061 tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg aataatgtgt
    36121 tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa gcaaataaat
    36181 aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca gaaatagcca
    36241 atggtataat gatccgaata atgatctgga tccggctaag accatcgatc attgctgctt
    36301 cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc gcagtaggaa
    36361 gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa cctagaccgg
    36421 aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa acggtgtaaa
    36481 agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg tatgccccgc
    36541 tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa ctatttttga
    36601 aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt tcaaaagtcc
    36661 attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc ttgaatgatg
    36721 tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa atcgtcgcgt
    36781 aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct ttcgtgagag
    36841 caagacgacc gctgcaataa tgatgaacaa tagaatcgat atagctgctg cttttccaag
    36901 atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga ctttattcgt
    36961 cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa caatcacagt
    37021 tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc aagcatttgc
    37081 accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa tagccaaaaa
    37141 tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca taaagaagct
    37201 agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc tatctactaa
    37261 cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa aaatcaaaga
    37321 aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc taaatctgaa
    37381 cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg ctacaactaa
    37441 gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac ttaaaatttc
    37501 aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg gcatatcgaa
    37561 aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta ataaaagcat
    37621 gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg gacgattcat
    37681 tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca ccattggcag
    37741 aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga catcttcgtt
    37801 tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga tatctcccat
    37861 tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa aagcactagc
    37921 aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt aaccatccag
    37981 ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga cccacttttc
    38041 catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt tttgtttctc
    38101 gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt gatctttttg
    38161 tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag aaggcaaaat
    38221 gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt tgatttctgt
    38281 cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg acaatgcttc
    38341 tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat ttgctccatt
    38401 tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag taggcatagc
    38461 aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta aaatatcatc
    38521 ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat accaaatacc
    38581 ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg aacgaacaag
    38641 gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt tttggactgc
    38701 cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac tgacttccat
    38761 tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga acgctgattc
    38821 ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat cagcaaccag
    38881 ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt gaatcgttgt
    38941 ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa atccagtagc
    39001 tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat tttattattt
    39061 tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg caatggataa
    39121 tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct gctgattgat
    39181 tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata aaactgataa
    39241 aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct ttttcttttt
    39301 ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat ggatctgtct
    39361 gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt cgattctctc
    39421 ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga atataaattg
    39481 ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt ttgcttctcc
    39541 attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt atctcatatg
    39601 gactggtcgt tccatctggg tttttcttgt agtttactcg ggtaccctct ttcttcagct
    39661 gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga ttcaatccga
    39721 taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg aaatcgatgg
    39781 tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga agggtcaaag
    39841 gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca aaataactga
    39901 tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga gctgcttcat
    39961 cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt atacaagaag
    40021 ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa gcgccttttt
    40081 ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt ttaaaattca
    40141 agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg tttttgtcaa
    40201 tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta tctgtatcca
    40261 gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat tcctgaaacc
    40321 aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt gtttcgcttc
    40381 tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga taatttacaa
    40441 cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca actctaaatg
    40501 tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt tgctgctctt
    40561 caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa tgcagtttgc
    40621 ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata gaggcggttt
    40681 tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac atcggattcc
    40741 cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac gcttactgtc
    40801 aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa agcttatgta
    40861 tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa gctattccta
    40921 aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta ttctaaaacg
    40981 ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt gcgaaaaaag
    41041 ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat atctctgata
    41101 aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg agcaaaattg
    41161 caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat cgtacttctc
    41221 atacgaccta tggcaatcct tttttttcag atgttattca atccatttct gccgtttctg
    41281 aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa gagctagaaa
    41341 aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta agctctacga
    41401 tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg gtaatcggaa
    41461 aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc caggatagtt
    41521 acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt ctccatgctc
    41581 cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc ttatttgatc
    41641 accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta gaagatagca
    41701 tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt ttttctaccg
    41761 atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag cagatacctg
    41821 atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt tcgccacagt
    41881 tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa cttttattcc
    41941 aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc gagattactc
    42001 tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa cagaaacact
    42061 gaggataagt cagttactct gttctttttt caatcactca tccctcgtta ccccctcccg
    42121 gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga taactataat
    42181 gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc acgttgtgta
    42241 taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc atgttatttg
    42301 gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt tttgttttat
    42361 tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct atcttcccat
    42421 caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga cttctcttgg
    42481 aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga tcctttccca
    42541 cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg tgacaataat
    42601 ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc gtcttctgca
    42661 gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac accgacttct
    42721 tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat cttacctata
    42781 ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc agcatccgtc
    42841 aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt ttctaatgat
    42901 gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc tgagactagc
    42961 gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg taagatatct
    43021 gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat tacttcgata
    43081 tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc acgaagcaag
    43141 tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc gattgatcct
    43201 tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat acgtgctgta
    43261 taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc tttggtacgt
    43321 gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat cactgcttgc
    43381 cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg agtgatatcg
    43441 tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc aggttcaccg
    43501 attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga gccagttgca
    43561 aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt gaagacagaa
    43621 cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc ttcggaaatc
    43681 aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc tttacgggtg
    43741 taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc gcggatggct
    43801 tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac atcttgggcc
    43861 acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc tgtatcggtc
    43921 atccctttac gagccccgtg agttgagata aacatttcca agacggatag tccttcacgg
    43981 aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat caaaccacgc
    44041 attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc agacatcatg
    44101 aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct gtcttttgca
    44161 gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag accacgacgg
    44221 aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc ttgtttttca
    44281 ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga atgtttgtac
    44341 cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa tcgtttgaag
    44401 acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa ttcttgctct
    44461 ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg tgtttggacg
    44521 gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat gatctcgttg
    44581 aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc agtaaatggt
    44641 ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac atatccgttg
    44701 cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc ttcgcgtcct
    44761 tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgggaagg agtaacaact
    44821 ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat acgtgcttct
    44881 gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc gaaatcggca
    44941 ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac taaaactggt
    45001 tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa tactgggtgt
    45061 tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc gattttacgt
    45121 ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat cacgaatggt
    45181 ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt caagaaaggt
    45241 ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa gttttgacgg
    45301 aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg gttacctggt
    45361 ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc ttcttgcagc
    45421 atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa acgtttcaaa
    45481 cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt tgcaaaacgt
    45541 ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat aacgtccata
    45601 accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc caaacggcgg
    45661 attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa ttcggctgct
    45721 tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc catagcagct
    45781 tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt caacaattgt
    45841 tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa ataaatgatt
    45901 tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg aatcccttta
    45961 aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg ttcacggcga
    46021 actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt ataacggata
    46081 cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat ccgctcacag
    46141 aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg tttttttact
    46201 tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg catactttcg
    46261 aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca aaccgcaaga
    46321 cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt atcttctgcg
    46381 gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc agctttcttt
    46441 tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac agtgatcaaa
    46501 tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat atccaagaca
    46561 cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga ttccggtaca
    46621 cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac acgtccgact
    46681 acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc gtaagcttcc
    46741 agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt acctcccaat
    46801 ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt gtcatcgacc
    46861 atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc aaatggttca
    46921 cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc ttcacgaact
    46981 gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac gtggatacct
    47041 aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt catacgtgaa
    47101 ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag gaatggcata
    47161 tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc ggccatttta
    47221 tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat gtttacgcct
    47281 ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac gataccgccg
    47341 ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc gccaaagatc
    47401 gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt gactttccct
    47461 acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat ttcgtctaaa
    47521 tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc aggtcctaat
    47581 tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta tacgtcatct
    47641 tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc ccaagtcatg
    47701 aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga tggtccgtct
    47761 gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg ttggttgtag
    47821 cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa tgcaccgttg
    47881 tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc atgtttacat
    47941 aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc aacccatggt
    48001 gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat caaggcacgg
    48061 ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac tacttgttta
    48121 ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc actttgagca
    48181 cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct gtttgcttgg
    48241 gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc agtaacacga
    48301 cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt atttactttc
    48361 gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt ttcgatcgga
    48421 cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc gcggtcacga
    48481 gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc gccaagaggg
    48541 tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt gatacttgcc
    48601 acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc ttggatcgac
    48661 attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg gttttgtaaa
    48721 agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc gacattgccg
    48781 ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc tggacgaacc
    48841 gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg ttcagggtct
    48901 cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac accgtcttct
    48961 gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag tgtttccatg
    49021 acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc tggatcaact
    49081 aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt tttgtttact
    49141 ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg agcattcagc
    49201 aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc gtaaacgtct
    49261 ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt ttcgactgta
    49321 ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga accaaatcct
    49381 aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg tacataagag
    49441 atgtcttttg catcagtttc catttctagc caagctccac ggttagggat aacagtagaa
    49501 ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc tggtgaacgg
    49561 actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc ttgttctgtc
    49621 attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt ctcgcggttt
    49681 gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc acgagcttct
    49741 gctactgtat actttggttc tttcaattca taatctacaa attccaaaga tagattgcca
    49801 ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc atctaagaac
    49861 cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac ttcactgatt
    49921 cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc agccaagctc
    49981 ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca ataatatttc
    50041 aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa aaaaaaccaa
    50101 aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt ccttatatta
    50161 aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt tttgcaactt
    50221 catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt ctgctttttc
    50281 gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat tcttaattga
    50341 cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa cttctatgcg
    50401 aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta gctactttag
    50461 ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta tctatgatgg
    50521 ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt ggctgagcag
    50581 tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt ccgacttttc
    50641 aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa ggaggaaaaa
    50701 gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc tttaggaggt
    50761 tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat tggcgtattg
    50821 caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga aggattagca
    50881 gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc ccaaaacaat
    50941 caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga tttgctactt
    51001 ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat cccgatgact
    51061 gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga aaaacctggc
    51121 ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg cttactgtta
    51181 aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga agcaaactct
    51241 aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa agtcaaagta
    51301 ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc taaagatgta
    51361 gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt tgtcggtgaa
    51421 gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt caaagacggc
    51481 ggtctagcaa cagtaggaat cgactacgaa agcttgggta aacaaacagg gatcatggca
    51541 gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc gaatgatttg
    51601 gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga cagcatcaaa
    51661 gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt ttcttatcat
    51721 ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc actttccgta
    51781 tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca gggatcgcgg
    51841 cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc tttttagggg
    51901 gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt cccgcccttc
    51961 ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg atgggtgcgc
    52021 cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca ttcggcataa
    52081 caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt gtatgtttac
    52141 ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc gataatctgg
    52201 caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt tatatgattt
    52261 ctaatggatg cattgctctt tctggctcct tgcttgcgca aaacaacggc tttgctgatt
    52321 taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc gcagaagttt
    52381 tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt gcggtcattt
    52441 atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat tcgaaacttg
    52501 cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa aaattccatt
    52561 tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat tggaaaatct
    52621 ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag gcattaatct
    52681 ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg gtaaatctac
    52741 tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt tattgaatgg
    52801 aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc gcgtattcca
    52861 agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg ctcttgctat
    52921 gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc gaacattttt
    52981 caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct cagaaatcgg
    53041 tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat tacagcgtcc
    53101 agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt ccaaaacggt
    53161 gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta tggtcactca
    53221 tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc aaggaaggat
    53281 cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat tattagcgtt
    53341 gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat cttcataatc
    53401 agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct ttccttcttt
    53461 cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc tctccttctt
    53521 cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca aggtacttca
    53581 attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc aactggaagt
    53641 ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct atcgttttga
    53701 gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa aagaaatcca
    53761 gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga cttctgaatc
    53821 tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga agttatttgt
    53881 tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg ctgaacctgg
    53941 acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg aattgcctca
    54001 atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa ccggtcgttc
    54061 tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct gtgggatttt
    54121 atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca ttgggatcgg
    54181 gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa aagccatctc
    54241 tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca aaatctggca
    54301 agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata aagaaaaatc
    54361 attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg tcggtcttgc
    54421 aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga agatgagttt
    54481 gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag atttcacaaa
    54541 aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag actctcctta
    54601 ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa tatctataaa
    54661 gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat cagtttacac
    54721 gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc atgggtaacg
    54781 ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta gctagtgcct
    54841 taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg catagcagca
    54901 tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca gttctcgtca
    54961 attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc aaaatcagca
    55021 gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg ctttgcttgt
    55081 tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa ccggctttcc
    55141 cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg gaaatctaat
    55201 tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc tctatcttcc
    55261 actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc aaaccgatcg
    55321 tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt tcctcctttt
    55381 tatttatcct acttactttt tcctaattct aggataccta cttatatgtt actacacacc
    55441 cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga aagaaaaaga
    55501 tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt aatattcttt
    55561 aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga gtataatagg
    55621 aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt actagtccta
    55681 gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga aacatacatt
    55741 cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt gttaggaatt
    55801 gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca tacagacgga
    55861 aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct tcatgaaggt
    55921 ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa ttcttcaggc
    55981 atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt ggatgtagca
    56041 cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac tccagatgat
    56101 ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt tgctaaattg
    56161 aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac tgctgcagat
    56221 tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac cgggttttta
    56281 ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat cattaatcca
    56341 aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa cgtacagtca
    56401 ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga tgcgaatatc
    56461 tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt tcaaggaaca
    56521 atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac gattatagga
    56581 acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat atttatcagc
    56641 gacaatggta cagatacgac agacacaagc gacagtacag atacatctac cacaagtgat
    56701 agctcagatt catcaacttc aagcgacagt acagatacta cctctaccac aagtgatagc
    56761 acggatacat cagcttcaag cgacagtaca gatactacct c
//
LOCUS       DAFGZE010000009        56062 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.009, whole genome shotgun sequence.
ACCESSION   DAFGZE010000009 DAFGZE010000000
VERSION     DAFGZE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.009"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(164..709)
                     /locus_tag="LTU54_000551"
     CDS             complement(164..709)
                     /locus_tag="LTU54_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL8295289.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(721..1413)
                     /locus_tag="LTU54_000552"
     CDS             complement(721..1413)
                     /locus_tag="LTU54_000552"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBL8295290.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1432..1716)
                     /locus_tag="LTU54_000553"
     CDS             complement(1432..1716)
                     /locus_tag="LTU54_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295291.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1729..2292)
                     /locus_tag="LTU54_000554"
     CDS             complement(1729..2292)
                     /locus_tag="LTU54_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL8295292.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2520..3182
                     /locus_tag="LTU54_000555"
     CDS             2520..3182
                     /locus_tag="LTU54_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBL8295293.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3306..4502
                     /locus_tag="LTU54_000556"
     CDS             3306..4502
                     /locus_tag="LTU54_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBL8295294.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4603..5979)
                     /locus_tag="LTU54_000557"
     CDS             complement(4603..5979)
                     /locus_tag="LTU54_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8295295.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(6016..6882)
                     /locus_tag="LTU54_000558"
     CDS             complement(6016..6882)
                     /locus_tag="LTU54_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HBL8295296.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6971..7996)
                     /locus_tag="LTU54_000559"
     CDS             complement(6971..7996)
                     /locus_tag="LTU54_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL8295297.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(8014..8688)
                     /locus_tag="LTU54_000560"
     CDS             complement(8014..8688)
                     /locus_tag="LTU54_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295298.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8872..9765)
                     /locus_tag="LTU54_000561"
     CDS             complement(8872..9765)
                     /locus_tag="LTU54_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8295299.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9839..11536)
                     /locus_tag="LTU54_000562"
     CDS             complement(9839..11536)
                     /locus_tag="LTU54_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL8295300.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11529..12842)
                     /locus_tag="LTU54_000563"
     CDS             complement(11529..12842)
                     /locus_tag="LTU54_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL8295301.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12842..16879)
                     /locus_tag="LTU54_000564"
     CDS             complement(12842..16879)
                     /locus_tag="LTU54_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL8295302.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16892..17350)
                     /locus_tag="LTU54_000565"
     CDS             complement(16892..17350)
                     /locus_tag="LTU54_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8295303.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17648..18166)
                     /locus_tag="LTU54_000566"
     CDS             complement(17648..18166)
                     /locus_tag="LTU54_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL8295304.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18168..18785)
                     /locus_tag="LTU54_000567"
     CDS             complement(18168..18785)
                     /locus_tag="LTU54_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL8295305.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18782..19645)
                     /locus_tag="LTU54_000568"
     CDS             complement(18782..19645)
                     /locus_tag="LTU54_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8295306.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(19632..20543)
                     /locus_tag="LTU54_000569"
     CDS             complement(19632..20543)
                     /locus_tag="LTU54_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295307.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20584..21918)
                     /locus_tag="LTU54_000570"
     CDS             complement(20584..21918)
                     /locus_tag="LTU54_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8295308.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22126..22956
                     /locus_tag="LTU54_000571"
     CDS             22126..22956
                     /locus_tag="LTU54_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBL8295309.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23200..23518)
                     /locus_tag="LTU54_000572"
                     /pseudo
     CDS             complement(23200..23518)
                     /locus_tag="LTU54_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23640..23834)
                     /locus_tag="LTU54_000573"
     CDS             complement(23640..23834)
                     /locus_tag="LTU54_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295310.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24339..24524)
                     /locus_tag="LTU54_000574"
     CDS             complement(24339..24524)
                     /locus_tag="LTU54_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295311.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25374..25715)
                     /locus_tag="LTU54_000575"
     CDS             complement(25374..25715)
                     /locus_tag="LTU54_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL8295312.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25715..25945)
                     /locus_tag="LTU54_000576"
     CDS             complement(25715..25945)
                     /locus_tag="LTU54_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL8295313.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26232..26570
                     /locus_tag="LTU54_000577"
     CDS             26232..26570
                     /locus_tag="LTU54_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295314.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26775..27014)
                     /locus_tag="LTU54_000578"
     CDS             complement(26775..27014)
                     /locus_tag="LTU54_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL8295315.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26990..27337)
                     /locus_tag="LTU54_000579"
     CDS             complement(26990..27337)
                     /locus_tag="LTU54_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HBL8295316.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(28069..28650)
                     /locus_tag="LTU54_000580"
     CDS             complement(28069..28650)
                     /locus_tag="LTU54_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8295317.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28833..29459)
                     /locus_tag="LTU54_000581"
     CDS             complement(28833..29459)
                     /locus_tag="LTU54_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL8295318.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29481..30812)
                     /locus_tag="LTU54_000582"
     CDS             complement(29481..30812)
                     /locus_tag="LTU54_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HBL8295319.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(30967..31368)
                     /locus_tag="LTU54_000583"
     CDS             complement(30967..31368)
                     /locus_tag="LTU54_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL8295320.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(31454..31660)
                     /locus_tag="LTU54_000584"
     CDS             complement(31454..31660)
                     /locus_tag="LTU54_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295321.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(31755..33257)
                     /locus_tag="LTU54_000585"
     CDS             complement(31755..33257)
                     /locus_tag="LTU54_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBL8295322.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(33275..33730)
                     /gene="argR"
                     /locus_tag="LTU54_000586"
     CDS             complement(33275..33730)
                     /gene="argR"
                     /locus_tag="LTU54_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HBL8295323.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(33828..34445)
                     /locus_tag="LTU54_000587"
     CDS             complement(33828..34445)
                     /locus_tag="LTU54_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBL8295324.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            34666..36039
                     /locus_tag="LTU54_000588"
     CDS             34666..36039
                     /locus_tag="LTU54_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8295325.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            36114..37226
                     /locus_tag="LTU54_000589"
     CDS             36114..37226
                     /locus_tag="LTU54_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBL8295326.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(37298..38935)
                     /locus_tag="LTU54_000590"
     CDS             complement(37298..38935)
                     /locus_tag="LTU54_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBL8295327.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(39103..39711)
                     /locus_tag="LTU54_000591"
     CDS             complement(39103..39711)
                     /locus_tag="LTU54_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL8295328.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(40095..41246)
                     /gene="queG"
                     /locus_tag="LTU54_000592"
     CDS             complement(40095..41246)
                     /gene="queG"
                     /locus_tag="LTU54_000592"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBL8295329.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(41265..42017)
                     /locus_tag="LTU54_000593"
     CDS             complement(41265..42017)
                     /locus_tag="LTU54_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295330.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(42158..43108)
                     /locus_tag="LTU54_000594"
     CDS             complement(42158..43108)
                     /locus_tag="LTU54_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HBL8295331.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(43109..43948)
                     /locus_tag="LTU54_000595"
     CDS             complement(43109..43948)
                     /locus_tag="LTU54_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL8295332.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(43945..44691)
                     /locus_tag="LTU54_000596"
     CDS             complement(43945..44691)
                     /locus_tag="LTU54_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL8295333.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            44929..46299
                     /locus_tag="LTU54_000597"
     CDS             44929..46299
                     /locus_tag="LTU54_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8295334.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEILYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(46354..47250)
                     /locus_tag="LTU54_000598"
     CDS             complement(46354..47250)
                     /locus_tag="LTU54_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL8295335.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(47364..47534)
                     /locus_tag="LTU54_000599"
     CDS             complement(47364..47534)
                     /locus_tag="LTU54_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295336.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(47531..48208)
                     /locus_tag="LTU54_000600"
     CDS             complement(47531..48208)
                     /locus_tag="LTU54_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL8295337.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(48299..49204)
                     /locus_tag="LTU54_000601"
     CDS             complement(48299..49204)
                     /locus_tag="LTU54_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL8295338.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            49379..50833
                     /locus_tag="LTU54_000602"
     CDS             49379..50833
                     /locus_tag="LTU54_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8295339.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            50849..51880
                     /locus_tag="LTU54_000603"
     CDS             50849..51880
                     /locus_tag="LTU54_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL8295340.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            51887..53074
                     /locus_tag="LTU54_000604"
     CDS             51887..53074
                     /locus_tag="LTU54_000604"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBL8295341.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            53230..54612
                     /locus_tag="LTU54_000605"
     CDS             53230..54612
                     /locus_tag="LTU54_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL8295342.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            54739..54969
                     /locus_tag="LTU54_000606"
     CDS             54739..54969
                     /locus_tag="LTU54_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295343.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(55169..55837)
                     /locus_tag="LTU54_000607"
     CDS             complement(55169..55837)
                     /locus_tag="LTU54_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBL8295344.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        16148 a        11704 c         9928 g        18282 t
ORIGIN      
        1 aaaaaatata acttaagttg ttgataataa agagatttat taacggaaaa agaataagtt
       61 tttttaaaat aacgtataaa aaagagcata aaacaaagtc gtttgtttta tgctcttctt
      121 ttattatttt gtgcttttta ttttatcaaa tcactttgtt tttttataaa atttctgcgc
      181 ctagtgtgga ctcaaaatgt ctcaatgccc attcatgtcc aacgggatcg aaacttgcca
      241 cggaatcttt gaaaatatgg agtttgtatc ctagattata cgcatcgact gctgtatgca
      301 acacacagat atctgtacat acgccggtca aataaatatc tgtgatctgg cgttctcgca
      361 aacggatgtc tagatctgtt ccactaaaag cggaataatg ccgtttgtct atccaataaa
      421 cattttcctc ttgctcatag tcttggtata aactttttaa agagccgtac aattcccgtc
      481 cacccgttcc tatcacatta tgcggaggaa ataagcggtt ctccggatga aaggaatctt
      541 ttggatcatg cgcatcaatg gcaaaaacga caaaatcctt ttgatcaata aacttctttg
      601 tatgagcagt cagctttttt tcaattactt gtcctgctgt cccagtagtc agctttccat
      661 cctctgcaac aaaatcatat gtgtagtcaa tcgagatcaa tgcgttcatc ttagtcctcc
      721 ttatacgaga tgtttgacaa attcgatcac catttctgct gatcgtttgc ctgcgtcttc
      781 gataaattcg tcaaagcttt gtgtcgccga gtgatctgct gtgtcgctca ttgcacgtac
      841 gatcaaaaat gggatattga actgacgagc tgtttgtcca acagcggctc cttccatttc
      901 gcaagctaat gcttcaggga aattcgttaa gatttctttg attttatctg gcgagtcgac
      961 aaatgtatca cctgtaacga tcaatccttc ttttgcgttc aaattcgttg cttttgctgc
     1021 tttaaccatt tcactgcgca aatatgtgct tgcttcataa tatagaggca ttcctggtaa
     1081 ttgtcctggg ttgtagccaa atcctgttgc atctgcatca aaataagcta ctttgtctga
     1141 tataacgatg tcacctactt gtaagccttc accgattccg ccagctgaac ctgtattgat
     1201 caccatgttc acgccgtatt gttggatcaa cagacttgtg gtaatagatg ctaagacttt
     1261 tcctataccg gaacgtacga cgatcacttc gtggttacct aatgaacctg aaataaataa
     1321 agcgcctgcg cgttcccatg ataatggttc gcttaggttc tctctcaaaa tttttacttc
     1381 ttcttccatt gcgccgataa taccgatttt catcttttta attccttctt tctatataaa
     1441 tgcgattgct aaaaaaacca cacataacat taggataaca ataataagag atttcatcaa
     1501 aaaagagttc catttggtcg tcttttcccg ttcgctgatc cttgttttcg tgattggttt
     1561 attctttttg ctttctttac gataaaagct ggcaatcttt ttttcttctt gctgataagc
     1621 tgtttctgct tttcgctgtt tttttaatga ctcgcttgct tgtgcttgtt gtcgcttgcg
     1681 aagttcgctt cgcgtaataa gcggtccttt ggccataagc cgatccctct actttccttt
     1741 ggtcaatagt tcccagtatt gaatcgcata aatcgtcttt gcatcacaaa tcaactgatc
     1801 tttcatcgct tgttgcgcct cttcaagagt tagatgatac aactccagta cttcgtcttc
     1861 atcttgtgct aaaggattct cgaccttctc taccccttgt gcatgataaa tatgaaggac
     1921 ttcatttgca aaacctggtg aaagatacat cgaagtaaga tggctcagcg acttcgcacg
     1981 atagccagtt tcttcttcta attctctagc tgctgtcatt tcgggatttt gtccttctcc
     2041 tggatcgatt tttccagctg gaatctccaa aatcactttt tctaaaggtt ttcgaaattg
     2101 tttgacaaga agcagacgat cttgttcgtc aaaagcaatg atcccgacac cgccaggatg
     2161 aaataccagt tctctcttcg aagttcctcc atctggcaaa cgaacttcgt ctacagcaac
     2221 atcaataatt tttccgctat agatttcttt gcgactgatt gttttttctt caaattgctc
     2281 attgttcagc atatcttctc ctgctttctt tgtaatcata actgcttttt tttcattttt
     2341 caatttttct caaaaactcc ttattagttt accatagata aaccttagat tttctctaaa
     2401 tatagaaaat ccttctttag tccgatactc tttctagtag tccgtatgct aaaataagta
     2461 tgtaatcgaa taacgaaaag aattcaatac tcaacgataa atgacatgga gggtttatta
     2521 tgataaaacg aaattggccg tggcttctag cattggtctt gctatttctc tttgtccgaa
     2581 acaatagttt ttcttttgtt ctaggacttt tattgatagg tgtagtttta gttttgtggg
     2641 gactctttgg ttcgcgcagg aaaaagggaa aacaagaaga aattcctgca ctgactgacg
     2701 aacttgaaac tcactatgaa aaaagcggta tgacagcgaa tgaaatttca ttttttcgac
     2761 aaacaatgaa tcaaacaaaa aatgagatca atcaattgca aatgaatatg cagcagacag
     2821 ccaaacttaa agcaatcgat ctccgccatg atccagtcaa agcagcgaaa gctttattca
     2881 aagaattggt gaaagaacca aaccgactgc atgaagcaag tcagtttctt tatacgcatc
     2941 tgccgaatct agttgatttg acaaataagt ataatgagat caatgaccat gagatcaaaa
     3001 acaaacagac ctatgaaaaa ctagaagaaa gcgcgcagat catcgaccag ctgtcagctt
     3061 taatcgcaca agattatcaa aaattcgttg ctgaggatct agatgatatc gacgtagaga
     3121 tatctgttgc gaaacagagc ttaaagcgtg ataataaata tgaaccagaa caaaaggatt
     3181 aactcttttg ttcataaaag aagactcgga acattcagat gatgtctggc tgtttcgagt
     3241 actttttcta aagtagagac attcatgtaa gaatcgagaa cagattcaac aaggaggaat
     3301 tttttatgga tcataaaccg actgaaacaa aagatgttac accagttaac gatacacttg
     3361 aagatttatt gaacaacccg ttttcaactc caacagatac gctgaccact tcacaacaag
     3421 cggagattga tcagctccaa gagcaacaga cagctgctcg tttaattgac aaactaccgg
     3481 cggaacgcca agaacaagct aaacagctag cagcaaaaat cgatgctaat gatgcacagt
     3541 ctgtcatcag ctacggatcg gctgcccaag caaaattaag tgaattctca caatcgatgt
     3601 tgaatcatgt gcaagcacaa gatattggtc ctgtaggaga ttccttaact gaactgatgt
     3661 atcgtctcca agaagccaat cctgacgaac tgcgtgccgg agaagggaac atcttccagc
     3721 gtgtcttcgg gaaggtcaaa caatcgatct atgaagtgac agcaaaatat caaaagattg
     3781 gtgcacaaat tgacaagatt tcagtgaaat tagataaaga aaaagacggt ctgctaaaag
     3841 ataatctgat gttagaacag ctttaccaga aaaacaaaga ctactttgat gcactgaata
     3901 tttatatcgc tgctggagaa ttgaaaatgg aagaacttca aacaacgatc attccagaag
     3961 cgatgaaaag agcagaagaa actggcgatc agatggatgt tcagattgcc aatgattaca
     4021 cgcaattcct agatcgtctg gataaacgta cgcatgatct tcgcttagct agacagatca
     4081 cgatccagca agcaccacaa atccgtttga tccaaaacac aaatcaggcc ttggctgaaa
     4141 agatccaagc atctatcgct acagccattc cgctgtggaa aaaccaagtc gtgatcgcat
     4201 tgaccttgct tcgtcaaaaa gatgcagtaa ctgctcaacg ccaagtctct gaaacaacaa
     4261 acgacttatt gaagaaaaat tcagaaatgc tgaaaatctc agccatcgaa acagcgaaag
     4321 aaaatgaacg tggaatcgtg gatatcgaga cccttcaaac cactcaaaat gatttgatcg
     4381 aaacaatcca agagacattg cgtatccaaa aagaaggaaa agaaaaacgt cgtcatgcag
     4441 aagtagagct tggacacatg gaagaagatc tgaaacaaaa actgttggat ttaacccaat
     4501 agatcacctt tcgcttgtta ctaaaacaaa cgcccaacaa cagtttatta caactgttga
     4561 tgggcgtttt tcttatcttt cttctaactt ctccattgga gtctatttct cattcatctt
     4621 gctgtgtcca tagccatagc caaaataaat gatcagtcca ataaccatta cgataccgaa
     4681 cacgatccat gtcacagttt gcagacgaag catcaataca atacaagtca agatggagac
     4741 gattggcaat agcggaacga atggcacttt gaattctcca gcttttggtt tgccaaacat
     4801 ttttctcaac ttcagtaagc ctagactaac aagcatcaaa taggcgagcg ccatgatatt
     4861 cgttagttcc gctaatacat tcaatgggaa aaatccggca aagaatacag ctgccacacc
     4921 agcggcataa gtcgcttttt tcggtgtacg atgcttttga tcgatttctg tcataaactt
     4981 aggaagtaag ccatccttgc tgacagcaaa cagtaaacga gcaagtgaat acatcatcga
     5041 gatcgttacc gtcagtagcg ttaaaattgc ccctacagcg atcaccgcgc ccactgttcc
     5101 atgtcctaca tacctcattg caaaagccac tgggtcattg actcctagtt cagtatacgg
     5161 aacgattcct gtcaagatca gcgtcacgat gatgtaaagg acagtagcaa tcagtatcga
     5221 accaatgatt cctcttggca catctttttg cgggtttttg acttcctcgg ctgccattgc
     5281 aacggcatca aatcccaaga aagcaaaaaa cacaagagct gcaccatcta gcacaccgga
     5341 aaaaccaaaa ggcatgaatg gctgccagtt atcaggttta acgaaaaata ttcctacaat
     5401 gataaacaac gcgatgatac caaatttgac atataccatg gcattattca aacgtaatgc
     5461 tttcttggct tcaagcgaca cgatgtaagt gacgaatatc actacgagca ccgcaatcaa
     5521 gtcaatgtat gtaccatttt ccggattgta cccgccagtt aatgcttgtg gaagttcggc
     5581 ccctaaactt ttcaaaaaac cttgcatata gccagaccat ccggaagcaa cagaggaaac
     5641 cgctagtaaa aattccccga tcaacaacca ccctgtcagc caggcagcaa attcaccaaa
     5701 tacaacatac agataggcat atggcccacc aatcactggc actcgcgaag caaattctgc
     5761 gaaacttagt ccagacaaaa tgacaacgat tgcagctaag acaaatgaaa gtgacagtgc
     5821 tggaccagca ttttgattgg ccgcaacccc tgtcaccaca aatattcccg taccgatgat
     5881 cgcaccaatt ccaagcatga tcaaatcaga cgtttttagt tcttttttca tctcactcgt
     5941 atgattgaga ttgatctctt tcttacgaaa caaatccatt gaagttcctc cccattttag
     6001 gttggaaaac agcgattaaa ctgttttcat ataccctaaa aggataccac ctaaaatgac
     6061 taaaaggcaa ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat
     6121 gaaaatccca cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc
     6181 taaaccaagt gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc
     6241 acagatcatg tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct
     6301 tgcgcctaag atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc
     6361 ccagttgacg atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa
     6421 acccttacca aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg
     6481 ggcagtcaaa tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc
     6541 tttcgtttgt gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg
     6601 aaaaccagta gacaatggta gccctacaga aacacccatg tacttcattc catggaactg
     6661 tccattttgt ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg
     6721 ggtgattaca ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt
     6781 cattcctagt gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact
     6841 accccatgcc aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt
     6901 tcacatgata agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc
     6961 taacgatttt ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg
     7021 ttgtcccttt tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat
     7081 tttttgctaa ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc
     7141 cagtaaatcc cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat
     7201 ctttaactga aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt
     7261 gatccggcgt atatttgatg gcattactaa gcaattggtt gaagataaaa gcgatccatt
     7321 tagcatcggt caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga
     7381 taaaataatt tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt
     7441 ggatgaggta gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt
     7501 attcatcgat ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga
     7561 actcgatcga acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc
     7621 atgagtcaat ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt
     7681 ggtgttcacg gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc
     7741 caccactcaa ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc
     7801 atcttctctt gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca
     7861 aagctaaata agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa
     7921 agatcgtcaa tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat
     7981 atttaaaaaa attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac
     8041 ttttgtttcg atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat
     8101 gttgactgtc aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa
     8161 tttttcccga cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc
     8221 atttttgctc aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa
     8281 cgtaatgcca ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg
     8341 aagtaatgca ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc
     8401 atctgccccc atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa
     8461 aatgatcggt actttcgata cttcacgtat tttttgacac caataatagc catccaaaac
     8521 gggaagatta atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac
     8581 ttgattgaaa tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat
     8641 caattgtcga atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc
     8701 catctttctt cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct
     8761 ggaaaatgtc attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt
     8821 gtctcttcca agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt
     8881 atttttcttt cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt
     8941 tagcactagc aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt
     9001 ctgttctatc tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa
     9061 tcggtgagtg ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc
     9121 tctaattgct aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata
     9181 agcgagcatc cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag
     9241 atccgtaaag agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact
     9301 ctctcctcca gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag
     9361 atcattcgtt tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc
     9421 gatcatatgt tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc
     9481 aaaaggatct ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt
     9541 tttcttttca tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg
     9601 cttttgataa ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa
     9661 tggatatgta aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa
     9721 aagactgatt tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt
     9781 atttaaaaaa actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt
     9841 atacttcaga cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta
     9901 ttccgattag taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt
     9961 tcatattcgg tactctttct ggccctggat tttctactcc tacgatctga atttcttcat
    10021 tatagcttcc ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat
    10081 aaccttctgg cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct
    10141 caaagtatcc atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa
    10201 ttctatctgc tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt
    10261 attcagaacg gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg
    10321 atgcatcata tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc
    10381 cttcttcata atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga
    10441 aatcatccgc ttctagttct gtggaaacct tcaaattata cgtgatcgtt aaatctcctc
    10501 cacgtgcatc tgctaatcta cctaaagtat ctactttggc tactccattt tcttctgtca
    10561 aaatcgtcac gacaaacccg ttgcttacta cttctactat ataatcttgt ccttttgtta
    10621 aaatcgtctc gccggctcgc acttctattt cctgatcata ataagttaga ccaactgtag
    10681 gcgtatctgt gattttgtaa tttttgataa cagcgcttcc gttttcaagg acagaatcga
    10741 tgaaataagg aataggtgct gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa
    10801 tataagaaat cgtttcttgt tctccagctt cattaataat cgttacttgt ctaaggtcgc
    10861 tttcctctac attcatctgt ttatctgctt ctctgatttc gtttttcgga tacaaatgca
    10921 catctgtatt gtatgtgtct ttccatgtag aaccatccca cacgccatcc accatatcag
    10981 gcatcatcac tggcatcata acaaccattg gagacgcgat tctggaaata tgagctggcg
    11041 aataagtttc taagaaaaga tagactttat ctctagaaga aggtgcttct tccgtcactt
    11101 ggacggtaga gaataccgct tctccagctt cattcgttgt ttgtttgtca atacttgtca
    11161 gtcgatattt taaaaaccaa ctttctgtgt gccgtttttg aatccattca atcgcttcgg
    11221 cagaattaag accgtcttct cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat
    11281 atacttccgt tacgtcaaac aaatcaaaat cgactcctgg aatattttct tcaccaaaat
    11341 ccgtagaaac caatccgcta ttcggtcgtt cttccggaac agatgagaaa ccttttttgt
    11401 gtaatgtaat cgtcacttca cttggttttt gtgcgacttc ttcggctttg actagttttc
    11461 cgttcccgaa aatccctatt cccgcagtaa cgagtaacag tacactcatt cccgtgacaa
    11521 tcattttttt attcattttt ttctccccgt tcttttttta ttaataaaac agcacctaac
    11581 aataaaagaa ttcctagtaa agaaatcaac gttcttgctt cattcgtttg cgggaatacg
    11641 gaccattttc ctgattgacc tggattgctt ggaccactcg gctctttcgg tttgtccgtt
    11701 gtttctttta cggtctcgtt ataaacacgt ggtgttgcgg attcataggc agagactgga
    11761 actttcccgg attctaattc agccatctcc tgtccgttaa ctactacata ttgtggttgg
    11821 ccttttgcat ccacccaaaa ttcagggatg atgacctcga ttctcttact tgcttcatcc
    11881 atctcgtaac cttccacacc ttttaattct tcaaaataat aggtgccaga aggcaagaaa
    11941 cgttgtcctg tcgtaacaag gccctcttta tccgaaatga atttgcttac atttttgtca
    12001 ttcaaaggat cattgtctgc tggtttgatc cattgatttt tcaagtcatt agcaggagcc
    12061 atatctaaat aatatttccc cccttctatc atctgatata gagcaaaaac agcaccttct
    12121 aatggttttc ctttttcagt tgttcctctt tgacgtccat acttgaagaa gtatggatct
    12181 cttagataac cgacattttt aggatagata tgaatctctt gcagttcctc ggttgattct
    12241 gtcgggtgat aaattggcaa aatagcagca attgggatag ctgtctgatc gatatcaaca
    12301 tccaatccaa cttctggatc aatcgattct tcgaaaatca aataaactgc atcccgatct
    12361 tggtttttct taggtgcttt gatacgtgca atcccgtctc ctaatccgcc aaccgtatct
    12421 ttcccagttg ttaccgaagt ccctatttgg acaagccttt cttctgtgat tagctgacgg
    12481 atattgtttc tgttcgtttc agcgatttgc tggacgaatt ctttaggagt catgttttca
    12541 gcagcttttt tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt cgctccattt
    12601 agtggcaccg tctgtgaaat gatatctgct gtttcccctg cttcctcaga gatagccaag
    12661 ccatcatttt catggtattc aaattgatcg ttataatcga tatctcgaat catccgttta
    12721 tgcaacacga attccacgac atcttcccct gattcttctg ccgacatcgc atgtggaaga
    12781 aggactgctg caagcggtag taaaacaagt aattgaagca tcaaacacaa cagctttttc
    12841 attaccttgc tccctccttt ttagattgtc tgacaaacaa accataggct gctcctgcaa
    12901 gcgccatgaa gaatgcagca agcaggatat agaataatcg tccggatcct cctgtagcag
    12961 gcaacggatt ttcaggatcg ttcccgacac taagctgtaa tctgattcga tttgtctcca
    13021 aaagttcata ttctaactct tgctgtagat caggcgtctc tgcttcttcg tttggataat
    13081 acgaaatctc aacttttagt gtacccgctt cttttcctgg aactacccga tcattttcta
    13141 tgtcaggttc ttctcgataa ctaatttcta aaataaagta acctggaagt tttctatagc
    13201 tgtcaggtga cttcacttcc ttgatccgat agaccccttc caacaagtct gaagcattga
    13261 atagggaaga atcgtttgga tctggtgcca agcctccctc gatgatttca tactctcctg
    13321 cactattttg tttctcaatt gaaaattctg cgccttcaag acgtcggcta ttcatactgt
    13381 caattttcaa aagttccaaa tcgattggcg ctagttcatc ttctaaggtt acttgtagta
    13441 caccatcgat gatttcatag ccttccggca agggagcagt ttcgtcttcg atgaccgtat
    13501 cttcaatgat aaacttacct tcttcattca gtgcaaactg gaacacttga tcagatagcc
    13561 gatacccatt tggtgcgaca gattccgaga cctcatattc accaatagac agatgcagag
    13621 gttggtcgta atttaaatcg acatactttt ctactcctac ggcacgagaa tagaagtatc
    13681 cgatcccagg caaacgtttc gattcttctg gttctaacgg attaccagtg tatgctcgat
    13741 acagcgggaa ggaagaatcc cccactttcc tgatatcgaa acctgcacca tctaatgtat
    13801 ggccttcatg gtctaatttt ctaactcgga tctcccgata atcaaacgta ttgtaagcag
    13861 tgaatgctac ccataaacga tcctcttcat ctgttgtata cgtgagatat tgccaatttt
    13921 cttcacctgt ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt cgcaagatcc
    13981 atgtgtcatt ttcatcaggt tctttgaaac cttctgggtt ctggctctca atgattttgt
    14041 attctgcata gttggtagtt tcttcactat ccttataaac aaatgaatct ggctctccga
    14101 ttggatgaat aactttctca ccatccacga ttacatattt cccgtcttca tctactgaat
    14161 agaacgtgag tctccctttc tcttcctcta cttcgttcac accagaaagc cctttcgcta
    14221 ctggatatcc gatttccagt ccgttaccat ctgtttcata aagttcaaac tctgcgccat
    14281 tgattggcat attactgcct tcaattagtt tattgatatt catgtggatt ggcttgaatt
    14341 catttcgcat ttccagtttg acatacaact catcatactc tgtgccttct atgttaggct
    14401 cttgtacaac agaaagcttg tgatattctc cttcataata ggaattcgga tctttacctt
    14461 ccggtccgcc gactctcgaa ccagatgtat attcatctac aagctcgtag tgacctaaca
    14521 tctcatcttc gttggctgca tattttttga tttggaattg atacgtctca tctactaact
    14581 gataacgtgg tggtccagcg atctctctga tccaaaatat gtcgctgact gctccagttt
    14641 catctcctaa aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg gtagttttac
    14701 ttgtttcgat tggttcggct tccccactct ctggattttg gtcatataat gcaaattcga
    14761 ctccagccaa cggctcgcca ttttgaccct tcttgctgac ttggaaatta cggcctttat
    14821 gttcgtaacg aatggaagaa gatgggaaat acatccgtcc agtagtgata tcattctcat
    14881 aatcctccag gttctgaata taggccatat tgttcactgt gcaaaaattt ccgtcttgag
    14941 ctcccgcatc aagttttact tggtacttga tcctgaccat ttccccttca cctaaagaaa
    15001 tcggagaaaa tttataagct tggttttccc gtccgacata cgttgtcgga gtcgccacta
    15061 cttctggtgg ggcttgtcca tgacgttctt catcccatat ttctggctct tggtacttca
    15121 tgccgttttc tcctggatct agatggtaaa actgaatatc tgccgatctg ccagctccaa
    15181 atggtgagac attaaaacca gtttcatcat aaagtgtggc ttgctggatc gttctcttga
    15241 aggtagaaga gatatcctcc aaggctgtaa caatgtcatt cttgttgttc gcattgtaat
    15301 atcgcggtgt tacgccatct tcttctaaat cagcaatatt tttcaataca tttcgtccaa
    15361 tagcatctag tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca atggagaaga
    15421 tacttggcgg gttctcttga tacttgtttt tcagccaatg atgataagca actgtgttga
    15481 tcgcagatga cttttcttgc gaagtaggac tgccattcgt attgttatta gaagaaccat
    15541 caccaaccca gtgagtcgct ttattatctc cttcttttac ctctccataa cgatagtgta
    15601 catttacttc atcttctgga cggttaaagt cagatgcata ggtaactgga tgagtaaacc
    15661 ctgcattaga agtattccca gaaaactcct caaaatttct gaacaaaacg tcattatctt
    15721 cagcaatctt atcttccatc acagtccagt tgctccaagc tccattagtg ctcgtacgat
    15781 aacttgactg aatcggtgca taactgaagg tcggtgtccc atctcccaca acgattagga
    15841 ttttttctgg atttctattt tctccgccat tgtctttata caatgtttca tagccatttt
    15901 taagccctag tgtaagcgga gttcctccga ttggtgtacg atttaatgct gtattctcac
    15961 ttcccgtcaa acgattgata tcatttgtca atggaaagat gtccgaactg atttgctcat
    16021 ggttattgat agctgtacta ttatggtaaa aattcaccat accgattcga atatccagac
    16081 tcgggtcaga gagcagatct ttactcattt cgtttactgc ctcaatcaac gcagcatttt
    16141 tagtttgact attggtccca gcggttaatt cactcatact cgctgactta tctagaacaa
    16201 aaacgatatc gattggactg atttcctttt tctcgccccc gatgacatct agatagacat
    16261 tgtaaagggt tgttgcatct tcaggatgta gatcagatgc tgtgctgctt ggcagaacag
    16321 gttctaccca ttttttgatg tacgccccgt tataatccaa gtaactgttt tcaaaattcc
    16381 gttcatttcc ccaaagctga acggtttgag ctggaacgtt tctatcttct tcgcctgcag
    16441 ttgatccatt attagtagca tagtcgtaat ttcgccagtt cgctcccccg tttgtataat
    16501 cactatcatc gatcaagttc tctccgtcat atggcgagcc tttgtctcct tgatagcgtt
    16561 cattgctttg tgtccaattt cctaacactt ttgtatagtc attcgttgta aacttcgggt
    16621 agcgatgctg ttcgttttca acttctccgc taggatagat atattcatag gggtcttgcg
    16681 tagctgttgg cccaagacgc tccaaaatac cgttgaataa ctggcgttga atgcgctgga
    16741 gtgcttgacc gctgggtacg attggttcgt ccagcttttc catctcattt tcttcgctac
    16801 gttgaatttc agcggaatct tctgtatcag tttcattttc ttgtaattct tggttatcta
    16861 ttctttcatc aaccaccact tgaagaggta gttacccttc gttcacatcg gggatctttt
    16921 ctgtttcaaa aatcaatgtg ccggtttctg cttcttttga ataaactttt ccgacatacc
    16981 attcttgacc atcgatcgtc tcgactttta ggtcttctcc atcttcatca taggaggata
    17041 gacctgttcc acgaacgttt tttacggtac caatcccggc ggctgccgca tcgattgcta
    17101 aacgtattct accttcattg gcactttcct ttttttcaaa agccagatgc cattcaaggg
    17161 tttcttcttt ttcaatatac ttcccttcaa tttttgcata gtcctcatct aagaatacct
    17221 catcctcctt tgtttctgtt gactcagcag ctacatccag ggtcgtcata tgatagactg
    17281 actggaaata tggtccaaat agcaaactga cagttactaa ctgcaaaagc ttcaatttta
    17341 accctttcac atccttatac tcctttcaaa aatatttgtc ttctttcaca acaatcaaca
    17401 aaacaatgcc catctaatta taatttaatt taattttaat tttaaatcaa atgatatcct
    17461 ttttagttaa aaaacaccaa ttatacaaca tttattatta aaattaaatt ataaaatttt
    17521 ataacgttct catttaacaa aacttaagtt attaagacaa atgaataaac aactacgctt
    17581 tcctcgaaac aaaaaaataa agcacaaatg cgcatttacg cactcatgct ttattcgtac
    17641 aattaattca gataacggca gcgcatagga tgccaataac acttcctaaa atagctcctc
    17701 ctaaaatgtc agtaggataa tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag
    17761 caaataaaaa ggctaatatt cctgcaactg gacttaattc gaacaacgca gctactgata
    17821 aaaaggctag cgctgtatgt cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa
    17881 ggacaagccg acgatttttt tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa
    17941 gcaaaccaac aaaaaaatag atcaaactgc cgataagaat ggctagacca tattctctag
    18001 tatcaggtat caagactaaa acagcagcaa ggagcaacca aataaaacca ccattagaga
    18061 tattactgat aaatttcaaa aaattgattt ttcgactatc cgttttccgt tgcttggcga
    18121 acttgttgat aatactatca tcgattttag caatcaaacg caacatgtta ttcctccttt
    18181 ttaagaacct tttcttgcgt acgatggata aaaagcagga cttgaaaata aaacaagaaa
    18241 ctgaaaccta ataaagcaaa atagattggt cctgcaaacc agaatgcgac aagaaggatc
    18301 aacgtgcctg ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca
    18361 gtaatccaag aagcttcttt ttgttccagt tgaagcaaga ttttgtaact aaagagaagc
    18421 gcgcttaata gaagcaaaaa caacgtagta tacatcaatg tatagccaat tcccccaccc
    18481 caatagcgga aaatctgata atcgatcaat agaagaagaa aataaacact tgctaaacta
    18541 tcttgctttc cataaagaga gaagttctca cgaaactttt tccagaatag tgaaccgagc
    18601 tttttctctt catttttttt gtaatgataa atcatataca gaactgttcg acttgcagtt
    18661 cctaatgtga aaacaggcaa gacgcacagg aaccatagta gctgtaaatg gataagacgt
    18721 ggaatccatt caatgatttc taagaaataa tcattggctt ttttaccaat cgatcgattc
    18781 atcatccttt tgaacctcct gtcaaatcca tcccttcaat gaaataacgt tgtgcaaaaa
    18841 agaacagcaa aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga taattccatt
    18901 gtgtcgttgc ttggttctga aaaacttgca agccaatctg taacgtgtat ttttcttgat
    18961 cttggatata taacagagga cctaagaaat cattccaagc tgcgttgaag gaattgatcg
    19021 caatggtaat caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa atatacaaat
    19081 gattcgctcc gtcgatttca gcagcttcga tcaattcatt attgatcgat aagtaaaatt
    19141 gacgcatcag aaatatattg aacgcattgc cgaagaacga tggaacgatc aggggcaaat
    19201 aagtacctac ccagccgatc ttggagaata atacatattg agggatcatc gttacaaacc
    19261 ccgggatcat catcgtagaa agaaccaaag caaacatcag ctttttccca ggaaaatcaa
    19321 gcttcgcaaa tccataagca ataaaagaat ttgaaaggac attaccgatc actactagca
    19381 cagtgatgaa taatgtattt cttgcatagc gccaaaaagg aaaggcggca atcgtatcca
    19441 gatagttttc ccatacgatt ttttctggga aaaaggtcgg aggataacga acgatttctt
    19501 ccattggttt caatgaagta aacaccatcc aaagcaatgg aatgatcaaa atgattccta
    19561 ttactgtcat gaggacataa cttagtattt ttttgtttgc cgattcacac tggtcttttt
    19621 gaaataatcc atcatttgcc gtcacctcca gaataataga cccacttagg tgcaagcttc
    19681 aaattgacaa acgtcagaac caacacgatc agaaacagga tcattgacat agccatcgca
    19741 taacccatat caaaagctac gaacgccttg ttccacatat gcagattata aaaaagcaaa
    19801 gaatttgcag gtccgcctgt tccatttttc gtcatgacat aagcttcttg aaaaatttgg
    19861 aaagaaccga tcgtagaggt gatcacatcg taaaaaataa taggagtgat taatggcaaa
    19921 gtaatacttt tgaactttcc ccaaacgcct gctccatcga tttctgctga ttcatataaa
    19981 gatctaggaa cattttgtaa tgtagctaag taaagtagca ttgctcctcc agaactccat
    20041 attttcatca aaatcaacgc aggtttcgtc cattgtggat caaacagcca agctggtcca
    20101 gggatatgga accagcccag aaatgtgttg atcaatcctg cagatggaga aagcagctgc
    20161 atccataaga tgtagactgc tactcctgat aagactgccg gtaaatagaa agctgtacgg
    20221 aatacactga ttcctttgat tttttgattg agtactgtgg ctaaaaacac actgaaaatc
    20281 gtcgtcagcg gtacattgaa gatgacgaaa taaacggtgt tgtataacga tttccaaaat
    20341 aaatcatcgt gaacgaacat tcttttgaaa tttgctagac caatgaaatt catcttggat
    20401 gtcaaattat aatcggtgaa actaccaata actgaaaaaa gcataggccc tagcataaat
    20461 accaagaacc cgataataaa aggcgagata aataaatagg cccatcttgc ttcttttttc
    20521 tttcggatag ataaacgctt caaattttct tgcctcctct gtacctagac agtcaaaaat
    20581 ggattattga ttttgcttga ctaagttttc tactgctttt tgtgcatctg ccaatccttc
    20641 ttttggtgat tttgttccta aaagaatctg gtcaatctgt tcattcacta atgtcgtgta
    20701 atccggagca tctaaaggaa ctggtgtgat caccgtattt ttaaagtttt cgtccgccat
    20761 ttgatagatc atttttcctt tatccgttag gttgtcatta ttcactaaat tctcattggc
    20821 tgtcttgctc gccatgatat caaaactttt ttcgccgaat ttttcttgta cttctggaga
    20881 agaaagatat ttgatgaatt cataagaggc ctcaggatct tttgctccgt atggtacttc
    20941 caagacgaat ccgccacccc acgaccaatg gcctgaacct tcttcttttt ctgggagctg
    21001 catcacgcca aaatcaaaat catccggtgc attttccatc aaactagagt aataattgat
    21061 attttgggca cgcatggcta ctaatccgga aataaatgga tcagatacac cagacccaaa
    21121 ttcggcttct aatttattga tcgtattttt cccataataa tcttgccagt cttggatcca
    21181 ctccaaggct tccactttat ttttagtatc aatcttgact ttttcatctt tgtcaaacca
    21241 actagtcccg ttatctgcgt ttagcgccca tacatcagcc cctaaattcc ataatggata
    21301 aaaaccgatg cgtgtaaatt cttttccttc ttttttatct agtttacgtg ctgtttcttc
    21361 taattcatcc caagttgtcg gtagatcttg ttcactgatt ccagcttctt taaatatctt
    21421 tttattataa aaaatgactt gtgtatctgt attgaatggg atcgcataag gatcaccttc
    21481 atattctact gtatcccaaa gctgcggata aaaatcctta gaagtgtctt tttctacata
    21541 tttggagaga ttagttgctt gctgtgcttc tgctctttgg gcaacggaat ttatgtcctg
    21601 tacaataaca tcgggtgcat ttccagctgt tattgcagat aaagatttag tccagatgtc
    21661 accccatggc tgatacttat aatttacatg gatctcgttt tgtgattgat tgaaatcctc
    21721 aatgatttct tcgatagctt cttttcgtgc accggatccc caaaaggacc agaaatcaat
    21781 attttttctt ccatcacttg ttttttcaga agaatttgct gcacttgaac tggcatcgct
    21841 tgcattactt gatccgcatc ctcctaataa aaacaatgct gaacttgcca gcaaccctgt
    21901 aaacaataat tttttcatat tttacctcca aattttaatg taaccgatgc cggttttaat
    21961 ataacgatat cattttgttc tgaaaataac aatttataag gtatgattct aaaaaaatta
    22021 ttaatgatac caatacttta tataattcgc gagcgctttc tttgaatttt ttaatctttc
    22081 gtagcagaat gagaatgtat taaaaataga ttaggaggaa ttttgatgtt tagacaccag
    22141 aaagaattgc agtttgaagc aaaaccagat cgtccgaatc cattaatcgc gaaatatctg
    22201 caggagctga tcggcggcca atatggcgag atgagtgtag ctatgcagta cctgttccaa
    22261 ggctggagct gccgaggaga agaaaaatat aaagatatgc tgatggacat tgcaacagaa
    22321 gaaatcggcc acgtggaaat gttggcgaca atggttgctc gtttattaga gggtgcgcct
    22381 gttgaagatc aagaagcagc gatgcaaaaa gatccaacaa tcggtgccat tatctctgga
    22441 atgaatccac agcacgcgat cgtagctggc ttaggacctc gtcctgctga cagcgtgggc
    22501 aatccatgga gcggtgccta tatcattgcg agtggcaact tgttagcaga ctttagagct
    22561 aatttaaatg ccgaatcaca aggaagactt caagtagcac gtatttacga aatgatcgat
    22621 gatcgtggcg taaaagatat gctgtctgtt ctccttgcaa gagatagcta tcatcaaaat
    22681 ctatgggaat gtgcagtgaa agaattagaa gaaaaagaag gttcaatctt agtcccaagt
    22741 actttcccgc gtgacaaaga acgaaaagac attgcttatg acttctataa cttctcagag
    22801 ggagaccaaa gtagtgaagg ttcttgggct agtggcaaag cactggatgg cgaaggcgaa
    22861 tatcaatggt tgaaagaacc taagatcgaa ggcgctgcac caaaattaga accagtaaca
    22921 ccaaaaatgt atgggacacc tccattcaag aaataaatat ctacccgtag aaacacccgt
    22981 tttttgttcc acacgctttg tgagcaaaac tgactaaagt cattaatcat aaccaccctt
    23041 caaaagggtg gttttctcta gagctataag ctcaaatcac cggccagcgc ctaaagacgc
    23101 tggctttcat attttatgtt caagccatat tgcctttgcc agttttgccc acctcttaaa
    23161 aggtttatgt actactcctt agcccttaaa aggatcgtct tactctcgta cactcagctt
    23221 atctaacgca ctatcatgtt tttcttgttc ttgaatgtac tttttgatgg tcgcttcatt
    23281 ttgtcctact tttgtcacat agtatccttc cacccaaaag tgtcgatacc caaatttata
    23341 ttttagattt gcacaatctc ttgatgatct cctgaatatt ggctcgatat tgattataaa
    23401 ttatttttct tctatacttt ggtgtgaata caatgtggta tttacacatc tactttgtac
    23461 gggctaaact atttcccttt attgctccac ccgcatagca agtggttttg tatttcaatc
    23521 gctcggcgcg ctcaattagg tctaaagaca ataaatctaa aaacccgctg cttcactgac
    23581 agaaacaacg ggttttcaga aaaaaatgaa atgaaaaaga tctcttacgg caatctattt
    23641 tatttttttg attttttata aatggaaaat gcttcaagaa gtccaagaaa taaagccgta
    23701 taaaaacaag aacctatgaa atctttattt attagatatt caacagaaat cccgataaca
    23761 ctagccaaca atatatttat cagaagtgta aaataaggat gctttttata gaatagaact
    23821 agtgttgttt tcatagactc tcctttcttc atatttccaa ttaatcacaa attcacggca
    23881 ttactagaaa aattaaaggg gcgcagaagt ggaaaaatac tcttcgactt tccctacaaa
    23941 ccaggccatc ttgtgtttaa taactatacc aaatctaagg tacctctaaa tctaaattcc
    24001 atttgtggcg gtgacctcca atttttaact cttctatctc ccatccagta tacataacct
    24061 tgatacttcg accacttata tatcgaacac acaaatacct atccattgca ggagtactac
    24121 ctaacttagt tgctacttgc atctaaataa cagctgaaaa gataggactt actcttagta
    24181 aagtatctga actattaatt tttaaactat catttttatc ctcaactaca tgcacatctg
    24241 ttgcaacttc actttctgtc tcatccagat aaatgattaa ggaatcggga attgcaatat
    24301 cttcagctga cgctggaata gttgagctaa attagtcatc atatctttat tataaaataa
    24361 ccaacagcaa aaaatacaat gaaaaacaaa cttggaacta atgtatcatt aataaatgtt
    24421 gaaaaattca gcaactcatt actgtatgat tttagcagcc caaccaacat ataccctata
    24481 aaagctaata agatggttag gaatccttta gaatttttac tcattcatag acacttcctt
    24541 aaataacttt tattatagaa ggtattgtaa ataataatat tgacaaaaag tgactcaaaa
    24601 taaaaaaaac cttacattat actggaatga actgtattga tagtgtacct aaaagctcta
    24661 ctgacataag atcgtgtaat cggtaaagta attttattaa ttatacggga tgtaatcaat
    24721 tagagaataa actaatcata aaaggtactt ttacattcat aattgtcgct ttgagattct
    24781 actttggttc actatctgac ccaattgcac attttcgatc attacaagta ttggtgtcgt
    24841 tagaacaact aaataaaaac tagcggtaga tttttcagaa tctatgctaa tttctgtatg
    24901 gatgttccag tgatggcaaa tttctctttt cattgttttt cttgtcgcat gatatgcctt
    24961 tttgtgttat tcagttaaac tatagaaatt ctttcctaga cagatcttga tcccaaattt
    25021 tacgtttttg agcatcgata tagtttaaaa tgacattact catatctaaa gagtttagat
    25081 tttttaggac taaaactgtc agttgttctt tttcttcttt ctgttaaagt attgtcgcga
    25141 cttttcgtat acgttaagat atgtcgtgaa aaaacaagtt ttgtgacaca aaaaaacgaa
    25201 tgacttgagg aaatcacctc aatcattcgt ttaaaatgct atatattctt tagtttaaga
    25261 tacggaaatt aatgccatcg accacactag tagaatgaac gacatttact tggccttttt
    25321 ttaatttcgt tatgtttagc atataaaaat attatagagt ttacttacaa tgcttaaaac
    25381 atatactgta taatctgatc aattttagcc atgtcttgca atggtaagtt ttctattttt
    25441 ttgagttttc gttccttaaa atcaagagat tttaattgtg taataattac ttgtcctttt
    25501 atatctagct cttcaggtaa tgagtaacgt gtcggtaaat tctttatagt ggaagtaatt
    25561 ggacatatga ccgaaaacat tgttttacgg ttaaattcat aacgagagac gactagtcct
    25621 ggtcgtcttt tttgaatttc ttttcctacg gagggatcaa aatcaatcca tacaatatca
    25681 ccttttttag gaatataaat tctttcttct gacactataa aatttctctc ccttcaggag
    25741 ttaaatcttc ccattcgtct ttctcataat actcggcctc ttccccacca ctaaatggat
    25801 cttcaatttt tggaactaac gtaattgttc catcatcaga atacacaacg atatattctt
    25861 ggttttctga aggtttcttt ccgttatcag aaggtaaagt taccactaca gaacttcctt
    25921 gtaatctaga ttttgtaaca agcattatca tcactccctt aataaaagta tactatataa
    25981 cactaagtaa cgcaaaattc ccctttgtaa actatctaga aaattgtttt gtttaaagta
    26041 tatcttattg gatataaata gaaataaatt gtagccagtt cattttttct ttaactaaaa
    26101 aaataatcca agaaacattg aacctatgga tccaaaagct acccaaatac tatagctaac
    26161 cccccgctac cttcaatttt gcagataaaa cgctttggga gaaaatttca aagacaacag
    26221 gccatcctat catgcccgat tataaacaaa aagctggcta ttccttttgc tctgacaaat
    26281 ttcaattggg aaagttcacg aaactagcag gtgaagaagt ttcaactgtt cggatcaaag
    26341 aatgcccgct tcaaatagaa acattggttc aacgaatttc aactagacaa gactttgcta
    26401 ttgtagaacg tgagatcgta gctatttttg tagaagaagg gattttacat gatccaactc
    26461 atatcaatgt ggataaatgg caacctctta tctataaatt cagagaatat acaactgcca
    26521 accagtcgtt aactttgaat tttagattcc aagaattcaa agagtcataa tgataaacat
    26581 cgttaggtat aaataaaaaa gactcgcagc acgagtaaaa aacaaataga agctatacat
    26641 tagaaaggtc gactgtgacc ctatagatgc agtatatgca gcttatgagc atttcttctg
    26701 ttctctttgt ttttgggatc tgtgctgaag tctttttcgt tttttgccgc tctttctttg
    26761 aaaaatcgat tatgttatac cttctccatt actaattcag gcatctgctg agtaatgatt
    26821 tttcgcgcaa aagaaatatc tgttacccaa ggttcgattt gttcttcagt caagacaagc
    26881 ggcattcggt catggattgg tgcgactgtg cggttaggtt cagtagtcat tatgattgat
    26941 tctgccattt tttcatcctg atggctacga taaaaaccgc caatatacac tattttttcc
    27001 ttatttgaaa acataaattt gtctttttct tcattccact cattgggaaa acacaacgat
    27061 tttgcaaaaa tggttgatga aaggtctttt tttcttctac tgtctctgca cgggcattaa
    27121 tcatcaattg tcctttttta aatccggtaa atccccactt catgatttcc ggagtgagga
    27181 tgttttcttc ctcttttctt aacgtcacaa caagatttga cggaaaaact tctccagctg
    27241 ctaattctgc tgcctgcttc ggttgtttcg tctgggcttc tcgataataa ttctgaagga
    27301 gctctgactc tagatcaaaa aaataacgac cacacatcct aatccctccc aatctttttt
    27361 tctatcatac actgcaacaa gaagttcgca ccaacaaaac gctttcaatt tttgtagaac
    27421 taaaaaagtt gtgacaagtg acaaactctc cagtgaatgg tattctcctg ccaactcctc
    27481 ggtcacaagt cacaatattc aacaaacatg agaagctgct ttttgaaatt gttccttgtg
    27541 actcggagtg aaacggcagg cttacgacct ctgggtaaac ggtgttcaaa agccagtttc
    27601 tcggtattaa gccaaattta tgcaaaaatg tagaaagcta ttttcacaaa atttttctta
    27661 atactcgaaa ctaagcgact ttttcacaac ctcttcctta aacggtgttc aaaagcttgc
    27721 atctgcggta ataagcccaa ccaaacgaaa aatatgagca gctattttta tttggctgtt
    27781 cttattactt gatgcagggc agctttttca caacctctcc actaaacggt gttcaatcag
    27841 ctaaccttcg gtattagctc aaaaaacggc aaaatatgag gaactatttt gccgtttgtt
    27901 tctctaatac tcaggtcaaa gcgctgattt cacaacctct aatacacggt gttccagaag
    27961 tagcttcttc gaaaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat
    28021 tccttcttat ttcttgaagc tgaccacttc tgtcacaacc tctaattatt aaagaacctt
    28081 ttgcgaagtt aacaatcttt ctttgatcaa ttgaaaattc tgcctaagta gtgatagatc
    28141 ctgtgtgact ttcaacaata agatacttcc ctggatttca gcaatcaacg ctttggcttg
    28201 acggtctgct tcttcatcgg acagtttttc ttcagaaagt gccaggatat tttgtttcgt
    28261 atacttgacc cattgattga ctaattctag tagcaacaga cggtattctt catccactgt
    28321 actcaactca ataatcaaat tacctacagg acagccatga taagtgatct gtgtctcatg
    28381 atcagcgaaa atccagtcca acatttcttc taatgcttct tctggctgtt ttccagcatt
    28441 taagatgtct tcgaacaatt ctgtctgcca tacgtccata tgcgcatgga tcaccgctag
    28501 acaaacttct ttttttgaag aaaagtaata ataaaattga ccttttcctg tatcagaagc
    28561 tgctagaata tctcggattg acgtttcatg atagcctttt ttataaataa gatctttggt
    28621 cgcttccaag atcttttcct ttgttttcaa tgatattctc ctttttcatt tctcatagtc
    28681 ctatattata cagtaaattc atcatttttt cttcttaatc gtttaaaaaa gactgtgcca
    28741 aattatctga cacagccctc actataaaac tgttctcata tagctagatg ttcattgatt
    28801 caagaactgc gaggacaaaa gcaattccta tcttatgata gttgctctaa tacttcctct
    28861 gttgtcatca cttgatctgc aataaagcca aagttcgtca acgctgcatc cattgcttct
    28921 tggccaggag caccaattgc atctgtcgct accacaacat tgaatccact ctctaccaat
    28981 tcacgcatat gggaatcgct acacaagttt ccgttcattc caccaagaat tactgtgtcg
    29041 attccatttt ttctcaattg gagcgccaaa tcattgcttt ctggtccaaa gattttatga
    29101 ggagagctga tgatcgtatt ttcatccaaa taaggtttca attcttcgat aaaatcggct
    29161 cctgaacctt cttccactga ttcatattga ctttttctag caaacatctg ctgctcaagc
    29221 atcatatgtt ctcctgcacc gccaaattgc catttatggt catgtgggta aaaataatga
    29281 ggagaaataa agagtttgta tcctttttct tttgcttgtt ttaccatttc aatcatattt
    29341 tcgatcgtat tgtatttttc taagatatct tttgtcaaac caaagccacc gcctgctggt
    29401 ttcaagaact cgacttgcgg gtcagtgaga actactgctg tatttttatt attgaacatg
    29461 gtggattctc cattcgttat ttattttaat acatcattta gagcttcact cattgtcgga
    29521 tgagaataga tttgatctct caataacgta taaggaagtt ttccattcat agctagcgaa
    29581 atcaagttga tgacttcatg agaatcttcc gcatagatac ttgcccctag aatcaagtca
    29641 gtttcagggt cgactaaaac tttgaataca cctttaggat cttctaatac ttgtgcttta
    29701 gggacaccgc ttgtcatata cttgaataat ttgtaattga cgccttgtgc tttggcttgt
    29761 ttttctgtta aacctacatt ggataaagtc ggagtgataa atacactgta aggcacagct
    29821 ttccggttat tcgttgttcg tttattctcg cccttcaact gatccataat gattcgataa
    29881 tcatctagag agatataagt gaactgcaat ccgcctttga cgtctccaat cgcccaaatg
    29941 ttgtctgcat ctgtacgaag gtattcatcc acagcgattg ctccgcgatc tgttagttta
    30001 acatctgtat tttctaatcc aagtgatgct gtacttggtt ttcttccagt tgcagccaaa
    30061 acttttgacg cttggattgt tacttcttgg ccatcttctg taaaggtgac ggcagctgtt
    30121 gattcttggt ctgctacttg atctacagat acacctagat ggaattcgat tcccgcattt
    30181 gtcatgtctt ctaaaatcat ttgcgcgata tcttcatctt cacgtggaag aaattcagaa
    30241 tgggcatcaa gaacgacaac tttagatccg tattcattga acattgatgc aaattccaag
    30301 ccaatatatc cagccccgat gatcactaat ttttccggta attgttcctg atccatcgct
    30361 tgcgtagaat caatcaagta gcgacttgta ttcaatcctt tgattggtaa aatgattggt
    30421 tcagaaccag tattgatgaa aatccgttcg ccttcaacaa tcactttttc accattattc
    30481 gtcacttcga tttgatggtt agaaagaaaa cgtgctgtac cgtccatcac tgtcccagtt
    30541 gcctcatcgc tgatcatatg ataatttttg gcgcgaagca tgcccgtcaa tttttctttc
    30601 cgagcaacag cctctgtaaa gtcgattcct ctttctccat tgaaaatcaa tgatttagaa
    30661 ggaatacagc cgatgttgat acatgttccg ccatacatac gtgttgactt ttcaacgact
    30721 aaaaccgatt caccttttga agctaacgcc ttcgctaaag tcttaccgcc ctttccaaaa
    30781 ccgatcacaa tattttgata tttttccatc atttttccct cgcttctttt ttacctaccg
    30841 attggtacaa aaatatgata accgataatt gtctattctc caaatcatct gctttatttg
    30901 tcaaaaaaaa ccagacaaaa agcggtcctt tcacgttttt agtctggttc gatttccttt
    30961 attcttctaa acaatcgtta cttctacatc gatatttcct ctagttgctt tggagtaagg
    31021 gcagaattca tgcgcatctt ctgcaatttt ttgggcttct tcttcgctga tgccatcgat
    31081 atgcacttct aatactacac cgatctgata gccgccatca tctaaattat aaagagagac
    31141 acgtgctttt actgtggact ctgctgtcat tcctgcttct ttgatcatat gttgtaccgc
    31201 cccattgaaa caagaagcat aacctgctgc aaataattgt tctggatttg ttccttctgc
    31261 atggattcct ggaggagtga ttttcatatg catcgagcca tttggtgcct cgacttctcc
    31321 attgcgcccg ccatggttga tcattgctgt ttcgtatgct tttttcattg tactgctcct
    31381 ttcgattgac catttgctac ttggctataa ttatagtgta tgacaagtaa ggcgctttct
    31441 acaaaaattc tgcttattcg aataccagaa tgccgtcttc tactattttt ccaaaaaaca
    31501 aaaaaatact actcgtgaac aacagcgtca tatttgttcc aaaataaata gcaatctttt
    31561 ctacataatc ttttatatga ttgattttaa gatacatata taccagaaag ttgaccccat
    31621 aaaccaatac tgttaaaaaa ataaccatat tcgttaccat tttttcctcc ttttgctggt
    31681 ctccctacca gcactcgatt taaccatacc actttttttg ccgtccaagc aactagcctt
    31741 tcaaaaagac tggtttattc tgctgttaat gattcaagat cagaaaaatg tccgaataat
    31801 tttgctaatt ttactaacgc caacttgttc tttccgaatt catcattttc tatctgtaat
    31861 actagtaacg gctcatgtaa gcttaaacgg atcaaaaacc agccgtttcc gtatggctct
    31921 tctagatcat atcgaatacc ttcttcattt tccgggtcga agttcatccc tttttcttct
    31981 tccaacagtc gcttcatttc ttctaatatc ttgtttcctt gttcgcgtgg ttcatcgctg
    32041 aacaatttta atcggatttc ttgtacttct acgggctgtt tcaactcttt gatttgttca
    32101 tctaatgtga taccttttct ttttaattct ggcaaaagca tcaggatctt tgcgatgaca
    32161 tatgcaccat catccagaaa atagttttcc ttgaatgcag cgtgtccgct tgtttcgatg
    32221 gctagctgtg tatctttccc aagacggtta agttctaatg ctttattgat tacatttcga
    32281 tagccggaaa tataacgaac ttggatgcct cctttttctt caatgaatct ttgtagatga
    32341 gaagaagtag gggaattcgt cacgattctt gttcctggat gctcttttaa tacgattgta
    32401 cttaatactg cgatcaaatt atttcgattg atcactgttc cagaagcact gacaactgca
    32461 gaacgatcca catctgtatc gaaaatgatc ccaagatctg cttcatttgc taaaacagcc
    32521 tgcttgatac tttccatcgc ttctttatta tcaggatttg ggatatgatt aggaaatcgg
    32581 ccatctggtt ccaaaaattg ggaacctgtt gaatccgcac ctaagctatt caatactttt
    32641 tcagcaaaga atccaccagc accatttccg gcatcaacaa tgattttcca accatttagt
    32701 ggtttgcttt ctagtgttcc aatccccttt ttaatttttg tgactaaatc ttgcgcataa
    32761 ggtgtcaaaa ggtctgcttt ttctacgctt ccagtttgtt cgctttttat tttttgctgc
    32821 tgtgatagga taaaagcaat atcttctttt tctgctcctc cttctgaggt aaagaatttt
    32881 attccattat aataataggg aagatggcta gcagtcagca taaccgcagc atcgcacgag
    32941 aactgttcaa attgagtgga cataaacata gcaggcgtag tagctaattc gaaatcgatg
    33001 acatgtatcc cttgttcttt cagactttgg atcaacgcct ctttcagtgc tggtccgctt
    33061 atcctactgt catgaccaat cccaatcgtc cattttttat ttggatcggt tgatttcttc
    33121 tttttcttta tccagtttgc tagtccccag ccgatctttt ttaccatgtc tgtcgtcaag
    33181 tttacttcat atccttcaac tggtaatgcg attcctcgaa tatccgatcc attttgcagc
    33241 gctttcatat ctttcatata aaagtcctcc tcatttattc cctcattttt tccagctttt
    33301 ctgcaaatcg ctgtgcttct tcttctgagc ggcaaataat aatgaatgta tctacaccgg
    33361 ccatcgttcc gacaacttca gggtaagcca cttcatctag ccaattcgtt accacatcgg
    33421 caccatttcg ttcagtcaat acgataacca tgaactgaac agactggata cgcaaaacct
    33481 cgcgtctaat tgctgtacgc aaacgttctt catttaaaga aacagatggc tggttaaata
    33541 atgtatagcg tactattttg ttttcatttt gactttttac taacttcatt tctcgaatgt
    33601 ctctagaaat cgttgcttga gtcacatcgg cgcctgcttc atgcagccgc gcaagcagct
    33661 cttcctgtgt cccaataggt tgttctgtaa tgatctggcg gatcagtgct tgtcgctgtt
    33721 tctttttcat attagacacc tctcaatgtt gattatagca aaagataagt aatatttctt
    33781 catattttgg tattttttac ttttattttt tgttttcatt caggttgcta acctttttct
    33841 attttaacac gcaaaatcgt cttcattttt tcaggtgctg attttcttgg atcgctgatg
    33901 taaatctctt tgtgttcttt agaagctctt tcatagcctt cttttgcgat gaatgcttct
    33961 aatttctcaa acgttgctgg ttcttcggca tagggcccga cgtgtaagat ttgggcacaa
    34021 agaccttctt ccatcttttt gaatactact tgttctttca agtcttccgg aagtttgcta
    34081 gaacgctgaa tggcttcttg tgctatcgct tctgtaacaa agtcaggctg tcggatcatc
    34141 aattgataag aaaaatgttc ttttttcatc actggttcat gcaaatactt ttcttgcagt
    34201 ccccattgtc cttccagtgg atagacagta tacatctgat aatcaggaat cagccaattt
    34261 tttttaggcg acatgcgtat tgcatagctg actgcatata gacaggctac tcttcgttta
    34321 aagtcttcct gattcgggtc gccgatccct ctgatgctaa agaatgattt tttaggaacc
    34381 tctagtattt gcgggctttt tttcatacta tagctcttgt ctttacgcca ttcaattttt
    34441 tccatttttc ttcctccgcg aaattccatt tagttattta ttatcatacc tctttttgag
    34501 tgacttggct aatcgtgttg acactatttt tcagtttgtg ctagaataag tgaagtaaat
    34561 aaatcgtaca gataacacgt ttcggcgtgt cgagagagag gcggtttcta tttttataat
    34621 agaaatcact ctcttttttt acgaaatttt ttagaggtga tgtaaatgac aaaacaaaaa
    34681 cagctgcttt tagtacctgc agtaattgca acagggatga tgtcttttgc gggcgtattg
    34741 atcgagacgg ccatgaatgt aactttccca actcttatca gacagtttga tttaacgact
    34801 gcacaagtcc aatgggtaac aacgatttat cttttgatga tttcgatcat tgttccgctg
    34861 tctacttatt tgaatcgcaa ttttagtctc aaaaaactct ttttagcttc aaatctgctt
    34921 ttcttggcag gtataatgat cgatttcttc tctcctacct tcatggtcct attgtttggt
    34981 cgattgctcc aaggtgcagc gacaggtatc gctcttccgt tgatgttcca tattatcttg
    35041 acatttgccc ccctcaatcg acgaggcgcg atgatgggtg tcggtacttt gacaacggcc
    35101 attgctccag caattggccc cacttatgga ggcattatga cttcacattt tacatggaat
    35161 catatcttcc tgtttcttgt gcccgttttg tttctttctt tgattatcgg ctggtttgct
    35221 attcccgaga tggcagtaca aaaaagtggt tatttggatt ggataagcgt tttaggaatt
    35281 tgtcttttgt ttagtggaag tctaacattt ttgagtacgt tgggaacagt ttccagctgg
    35341 attgctttaa tcatcggcgg tattggctgg ttcctttttt actatcgaag taaaaaagtg
    35401 gagcatccac tgatccgact gtctattctt tcaaataaaa cattccgctt gtttttgatt
    35461 ggcttcttag tttatcagtt tttattactt ggtgtatctt ttgtcttgcc gaatttcctt
    35521 cagatcgttc aaggaagtag tccttttgtc gctggattag cgatgcttcc cggcgcagcg
    35581 gttggtgctt tgctttctcc tttttccggg aaaatgctgg atcaatatgg tccgaagaag
    35641 ccaatttttg ctgggctgat tctttctttg attggttggc tagcattatt cctactcata
    35701 ggacacgctt cagtgggtgt attgattgct tgtcatgtgt tttatatgat cggtatcgga
    35761 ttatcttaca gcaatatgat gaccaccgga atgaatgcac tgaatgaaga actacaagga
    35821 gacgggaacg caatgttcaa taccttgcag cagttttctg gcgctgtggc tacttcatta
    35881 gtagcggtca ttatcaatta cgtccagcat catacttccc ataactatga agtgtctact
    35941 acattaggat caaaagcagc attaggcgtc ttgttgctgt tgcttctcgt aagctttgct
    36001 cgattcgcct attatctgtt ctttgctaaa aaagcgtaga ttccatatac ggcaggtgtt
    36061 gcatctgccg tttttttgtg tgaaaatggt tgtttgtaaa aggaggcttt tttatggaaa
    36121 aaattgccat tatcggtggc gggatcattg gtatgaccct agccagttac ttagatacat
    36181 caaaattcaa tgttgcttta tttgatgaag ggacaggaca ggcaactaaa gctagcgctg
    36241 ggattatctc cccttggttg tcgaaacgca gaaacaaaca atggtatcgt ctggctaaag
    36301 acggcgctgc ttttttccct aaacttataa aagatttcca acttgatcaa acgatttacg
    36361 cacaatcagg gacacttttg cttcgacctg aaaaagattt agctgaattg gctgttcttg
    36421 ccgagaaacg aaaagaagaa gcgccagaaa ttggaaacat cgaatggctt tcacctgaac
    36481 agacatctca agcacttccg ttattgaaac cagcagtttc actgcgtatc agtggtggtg
    36541 gtcgtctgga tggaaaagca tatttagaac atcttggtca gcgtttagca caaaaacaaa
    36601 tggagcagat ccaggcaaaa gtcactttgc ataagaagag cgattcttgg ctcatcaagg
    36661 gaccgtttgg agaatacaca gctgatcgtg taattttgac acctggcccg gcacttaagc
    36721 ctttattaca agaattaggc tatgctgcag atatacgtcc gcaaaaaggg cagctcttag
    36781 catttcagac atcttataag atgagtcaag actggcctgt tgctatgctt gacggagaag
    36841 ctgatttcat tcctttcatg gatggaaaga tccttttagg tgctacccat gaaaacgatc
    36901 aaggctggga tttggctcct actagcgaag cttataaggg attgactaag agtacagaac
    36961 agttcttgac tgatccagat cagataaaaa actggccata cgagttccgt gtaggtaccc
    37021 gtgcttatac gtcagatttt tccccatttt tcggcgtact gccagatgat ccaagcttac
    37081 tggctgctag cggtctaggc tcttctggct tgaccacagg tccttatatc ggctatctat
    37141 tagccaactt cttaaatact ggtcattggc ctacggaaga ttatcaaaag gacatctcca
    37201 cctatattac gaaaaatcca tcatagaaaa acagcttttt caagtaagga tcccgagcga
    37261 tattgcgcat ggattacttc ttgaaaaagc tgttatttta gttcaatttc tgaatcaaat
    37321 tctccaaact ttcttttgct tcatctggca agagatcttt tcctatctga gcttcaatga
    37381 atcgtaaacg aatatcaagt cgttgtttca ccagattttt ataggacgta tcctcaaaag
    37441 aaacaggaaa atctggtata ggtaaatagt ggatgtcttc aaatggacca aacgacttga
    37501 attctgccgt ttgttgcacg attctttcaa cgattgacaa tgtcgtttca ggtttgattt
    37561 ttttatcatc gaaatatcca gtattcagga catagcaggc tgtttgctgt ttcaaaaaca
    37621 actctcgaaa agcttgatag tcttccctaa gaggatagac acggaaagga ttagcaaatg
    37681 gttcaatgac tagctcatct agctgtcttt ttgtagtgat attttccgcg tctgatcgtt
    37741 ttgttgctaa tgttgcacca aaagacgctg ctaaaactgg gtcattgatc ttgataattg
    37801 gcggaagcga atcatctttc atgatccaaa aaacagcatc gatcttttcg ctaagatgat
    37861 cgactcggtc gggtgtcgca taacgtgatt tgactgtccg cccatttccg tttcgtatat
    37921 ctcctgttac taggactttt tctcctttac ttgtacatgt cacgcccaca ttctgacaag
    37981 taaggaaaga agaaatggcc ggatcatcag acggatagtc ttgtgtttta tcaaagtaag
    38041 aaggttccaa ggcagtagca aaaccatttt ttcgagaaat aacgaaagca tcgtcatgca
    38101 agatcgaaac agagtgacct tccgtatgtt ttgctaaggt aatcgttgat tttccagaac
    38161 cggacaaccc aaatacagcc atcgtatagg tctggtcatt actagcatat tgtttcatcc
    38221 caccgtggca ggcaataaag ccattgcgat ttcccaatgc ccaagcaagc gtcaaagtcg
    38281 ctttttttaa ttctccaaag taacgtaatc ctagcacagc cgctgtatta tggagagcat
    38341 caaacaaaat gagtccattt ggaaatgttt catgtgtcca ttcgggatca caaaagatat
    38401 aaagatctcc ctcatcaaag gaaacagact tttgataagt ctctaagatc ttcgaattcg
    38461 ctggctgaaa attcagcaaa taagaataca aattgttctc aaatccttct ggaatcagca
    38521 aatgactttg cacagaaaat tcttcagata ggccaacgat tacttttgta tggtaaaact
    38581 ggcgcttgcg actttgatac acagcttctc ttaaaatttt ctcgtatttg ctttgttcct
    38641 tgtctaagtc ctcaaagatc cgccgagcac ttgctgttct tcccacaatg cttccatcat
    38701 tgaatactag tgtcctaaca tcagctggta aatctagttc ttcagcatgt gagattgtta
    38761 aatctgtgat gatcgttcct ggactccctt ttgctagttg ataagctgtc ttcctatcaa
    38821 aaacttcatg aacattatta ccgtaaaatg tcgtttctac aatcgatcga atactggata
    38881 acagtggatt tccacttgtg atttcgttta attcgagact ctcttttgta ctcatggctc
    38941 ctcctcattc cctaaacaaa aaaacgtttt ctttattata cattattcga gtttttattt
    39001 gtactgctgc gctgtgggaa gcaaacgagt tatttttcac acaaaaaaac cgagaacttg
    39061 aataaacaag tttctcggtt tcatcatttt ccttttatcg gcttagtacc agccgtttgc
    39121 taaccagaat gttttagcag catcccatga accataacga gatgttacat attgttctgc
    39181 tacacgttct tgattggcag ctgaatagtc gccgtttaag tatgaagcat ctaattgata
    39241 acggccgatg taacgtccgt tagtagctgt gtatgaaccg cctgattctt tttgtgcgat
    39301 ccattctttt gcagagcttg aagttggcgc tgttgcagca gctggggcag caggagctac
    39361 ttcaactgtt tcaacaggtg caacgtatgt atgttcttgc gttgctgttt cagttgtaga
    39421 tgctgtattt tctgttgttt tgttttctgt tgttgctgaa gaatcatttg aagttgggat
    39481 tgttaattgt tcaccaacat aaatgcggtt gatgtttgcg atcgaatttt tttcagcaat
    39541 tgcatcgatc attgagttgt ctcctgcaaa tttttgtgag atcttagata atgaatcacc
    39601 tgattttact gtataaactt catcagcgtg agcatttgta gttcctacaa acattgcagt
    39661 agcaccagca gcaaaagttg tacctaaaac aattttctta aatgaattca tattaaactc
    39721 ctttttttct ccgtttttta ttctttcgac gctattaact ttatcattta gaaatatttc
    39781 ataggtaaaa gaaacatgac atttaatgac aaaataacga attatttaat atttgtaaca
    39841 tttaaacaaa tatttttttg atacaaaacc ataaaagcct tgttgtataa gcaaagtaat
    39901 cgtttttcta tgttttttta tttttgaatg aaaaacgcag attctgtaac attcactgca
    39961 ataatccata aaaaaaagca aaaaaatttt tgatttttat caaattctct taaaaaaatg
    40021 taaaaaatac aaaaaaacca gtaattgtta caacaattac tggacaaaaa ctttgaataa
    40081 tgttaactat ttttttattt taactttctc cgcaataatt tctgggcatg ttccaattcg
    40141 tttataacga cttcttctgt ttctttagct aatatttcat ctaatacatc aagccatttt
    40201 tccggttctt ttgttcctag acgtccaata gcccaagctg ctgttcctct gatgacaggg
    40261 cggacatctg ttaaacattc tgaaatctca ggcaatgccg ttcgatcacc tagattagct
    40321 aaagcaatca atgcgtttct ctgcaaaggt ttcttcccac gccatgaacc agctaaatgc
    40381 ccaaattgct gtttgaagtc tttattagac atcgaaagca ttggctttaa ttttggatat
    40441 atttcgtcta cttttggttc catctcttca tgaaaatgaa aatcttttcc ttgattgtaa
    40501 gggcagacta actggcagat atcacatcca taaatgacat ttcgcatttt tttacgatat
    40561 tcttccggca tcatcccttt tgtctgagtt tgataagaca aacatcgctg ggcattcatt
    40621 cttccgtcac caagcaatgc ttttgtcgga caagcagtca cgcagcgcat acagtcaccg
    40681 caaccaaatt ctcctggttt atcaggctca aatacaatat tagtaacgat ctcaccaaga
    40741 tagacaaatg aaccgaactc ttcagtgatc aacaatccgt ttcgtccaat aaatcctaaa
    40801 ccagctcgct gtgctacagc gacatcgatc agttctccag tatctacttg cggagctaga
    40861 cgccattcct cttctttttg ccatttttct gctcttgttt ggatgaattc gatcaatcgg
    40921 ttcaaacggt ctctgagtat atcatggtaa tcgatccccc aagaagcacg agcaaactgc
    40981 ccgcgttttt catccctagg cacttcttca tggatcctag taggatacgc aagtgcaatg
    41041 gaaatgatcg atttcggctg atcaaatgtc atttctggat aaagacgctc atcgatattt
    41101 ggatgttcaa agccagaagt atggccagct gctttttgtt cttccaacga ttcttttaat
    41161 gaatcaaaag gttccgccgt ggtgaacccg attttgtcaa ttcctaatcg tttgctttct
    41221 tgtatgattt cttcttttaa tgtcatggtc ttcctcgctt gctgttaacg gctgtataac
    41281 ccataatata gtttttcttt tttcagctgg acaaactgga tctgctgatc ggcaatcttc
    41341 ttttcttcca tccatttttt tcttgcagca aatacttccc aataatttgt tgaaattaat
    41401 tttcttttgt tatctaatgc attaaaaaaa ttcaatgaaa ctggttttgt acttaacagt
    41461 tcatggagcc ttacctcttc ccatggcgaa aactcagaca gatcgcggga atacaatact
    41521 tcttcataga gatttttttg ctgtgtgtac gtgaatgttt gcgggatttt cccaggccta
    41581 gcaatttcta ctagacgatt ataaaacata ttcattaatt tgctgttggc aataaattga
    41641 cttttataat aggccgactg gatctttttg attactcctt ctgctttgat gactgaaaat
    41701 aatgcaggcc atgctttgac ggttatttcg tcgttttgtt taactaatac gccaggtgtg
    41761 aagccgactg gagaaaggaa cacaaaataa ccgataccgg tattttcatc taaaggctga
    41821 aattcatcaa acaaccattt gcctatgtac agctcttcat ctggttctgt tgtctcattt
    41881 tgatttgcta tgaataattt agctaattct gcttcaagaa attgaaggtc ttgatccttg
    41941 tcggtatttt cctgctggct gatcaatcga aagacttctt ctttaatcgt atatttatct
    42001 ttacgtgtaa aattcatagc cacaccttcc ttccttactc taactataac ggaatttctt
    42061 caaaaaaggt atctgtacga cgaaaaacag cataattttt tttcatatct cttataaaaa
    42121 atgcaaggtc tgctcctcaa gctagacctt gcacttttca gctttctttt gtttgtgaca
    42181 aaccttcatg gatcgtttca atattccatt tcatcatctg atagtaagaa tctccttgtt
    42241 ctccttcttt tgctatggaa tcagtgaaca gtttatcata gattttcaaa cccgtttctt
    42301 ttgctacccg ttccatgctt cttgagtcta cactggtttc cacgaataaa accggtactt
    42361 cttttgctct tatctgatca atgatcgctt tcatttgatc aggcgtacct tgactttctg
    42421 tattgatctc ccatatataa gctgctggca aatcatatgc tcgtgaaaaa tacttgaatg
    42481 ccccttcact tgttactaat aattttttgt tctcttcgat agaagcaaaa ctttccttcg
    42541 cttgtgtatc taattctttt agtttttcca catatgcttc tgcatttttt ttatagagtg
    42601 tcgcattttc tgcatctttt tcagagaata tacgtgcgat ttcctccaca tattttattc
    42661 cgttagatag gtctagccaa gcatggggat ctgcttttgt atgttcttca ccgctagtta
    42721 aataaagagg ttctacattt ttgctaactg caaaataatc ttttccttct tcttttttag
    42781 ccgtttccat caagttatcg aaccagctgt tacctgtttc aagattcaaa ccgttgtata
    42841 aaataacatc tgcatcgctt gcctttttga tgtcttctgg tagtacttca tattcatgcg
    42901 gatctgttcc gactggtacg atactgtgga tatctatttg atctgttccg acctcttttg
    42961 ccatgtccgc caagatcgaa ttggtagcca cgactgacaa cttttcttta cttccttcat
    43021 ccgcttggtc agaagcctgc tttccgcaac cggctaatac caagaaacta actgccaaga
    43081 ttaaaaagag tgattttttt gttttcatct atctgcttcc tttctgaaat aaaattcctt
    43141 ttgttggaga aaataaaaaa gcaaggataa aaaatgcggt acatgctaac acgatcgtag
    43201 ctcctgatgg ccaattgtaa ctataactga aaaacacacc gacaactgca cttagcatac
    43261 cgattcctgc agctgtaatg atcattttca gtaaatggtt cgttagcaag taagccgtgg
    43321 cggctggtgt gatcaacatg gcaatcacca agattgttcc tactgtctgt aaactgacta
    43381 cagccactaa tgtcaaaaag aacatcaaaa gataatggat cagcgatgtg ttcagcccat
    43441 aagcttttgc cattgtcgga tcgaatgatg tgatcttcaa ttctttataa aagaaaaaga
    43501 caaatatcaa gacgattcca ctaacgagtg ctgtcagcaa caaatcgctt tccctaacag
    43561 ctaagacatt cccaaataaa atatgataca aatctgtcgc actctgggca tatgaaatca
    43621 agataatccc taatgcaaaa aaggagctaa agacaattcc aatcgctgta tcatttttca
    43681 atcggctccg ctgagtcacg aaaccaatca ctgctgctgt tagcatccca aaagcagtgg
    43741 ctccaaagat gtaactaaaa ccaaacatat aagaaatggc tacacctgga agaactgcgt
    43801 gagagatagc gtctcccatc aaagacatcc ctcgtaaaat aataaaagaa ccgatgactc
    43861 cagaagcaag tcctacaagc atggaagtga ccaaagcgta ttgcaggaat tgataattca
    43921 ttaatccatc aataaaggat tggatcattt tttctctcct ccttttacta aatgtctgat
    43981 gatctcacca taggccaatt gaaggttctt cgatgtaaag acttcttgta ctggaccgga
    44041 agcgatcagc tgtttattta aaagaataac ctcatcgaaa tattcttcta ctttatgaag
    44101 atcatgatgg acgatcaaaa tcgtttttcc tgaattcttc aattcctgca agatgtcaaa
    44161 gatctttcgt tcacttaacg catcaatccc tacgaatggt tcatctaaaa agatccattc
    44221 tgctccttgg gctagagctc ttgcaataaa aactctctga agctgtccac ccgatagttc
    44281 gctgatctgt cttttggcat attcttccat ccctactttt ttcaaagctt gtcttgcacg
    44341 ttctttttct tttttcccag gtctttgtcc aattcgtaaa gatggatatg ttcctaaaag
    44401 tacaacgcct aatacatcaa ttggaaagga aagatccaat tcacttcgct gttccacgta
    44461 ggctattttt ttacggactg agtttaccga ctgtccagaa aaggtgactt taccagtagt
    44521 agctggtatc aactctagca aagatttcat aaatgttgat tttcctgcac cgtttggtcc
    44581 gataataccg gtaatttttt gtggatttat cgtcaagttg atagttttca aaacagtttt
    44641 cccttgataa gcggctgaaa ggtcttgaat tacaatggcc gtacgttcca aatcttacac
    44701 tcctttttag gtttacctaa ctttttattt aaatatagtt taactaaaat ggaaagcatt
    44761 ttcaagtaga atgatgaaaa agtttttttc gaattttcct cgcattttat gaaaattttc
    44821 tgtagaatca catcaagagt tcaataaggc taacctaata atgaaaaaca tgtgttgaaa
    44881 cgtttgatta ttttttcttg ttgactcaac aacgaaagga gtttttgtat ggaagaagaa
    44941 aagttaactc gaagtcttag tgcccgtcat atccaaatga tcgcactagg tggaacgatc
    45001 ggtgtcggtc tgtttatggg tgcctcctca accatacgtt ggacagggcc ctcagtgatg
    45061 ggagcctatg cgattgcagg attctttttg tatttgatta tgcgtgcttt aggcgaaata
    45121 ctttacgttg atccttcgac tggttctttt gcaaattacg cgagcgagta catccatcct
    45181 gtggctggct acttgaccgc ttggagtaat atttttcaat atattgtcgt aggaataagt
    45241 gaagtgattg ctgtaggatc ttatatgaat tattggtggc ctgatttgcc ggcaattatt
    45301 cccggaatca ttgtcgtctt gtttcttatg ctggcaaatc ttatttctgt caaggcgttt
    45361 ggcgaattag aattttggtt ttcgatcatc aaagtcataa cgatcatttt gatgatcatt
    45421 gcaggtcttg gggtaatctt atttggattt ggtaatcatg gtcaggcagt agggatttca
    45481 aatctttgga aaaatggtgg attcttcact ggaggcgtca aaggattttt ctttgcatta
    45541 tcgatcgttg ttgcttccta tcaaggaatc gaactgatcg gaatgactgc tggtgaagca
    45601 gagaatccta aaaagacgat cattgaagct gtccagtcca ctatcggtcg aatcttgatt
    45661 ttttatattg gtgctatttt tatcattgtg agtatctatc cgtggaatca attgagtgag
    45721 attggttcac cttttgtaca aactttttct aaagtcggca ttacatttgc tgcaggactg
    45781 attaattttg tcgtgattac agcggcatta tcaggttgca attcagggat tttcagcgca
    45841 agtcgtatgg tctatacact tgccataaat ggaaagatgt ctaaaaaatt tctgaaacta
    45901 tctcgtcacg gtgcgccttt ttatcctgtc gtagcaatct caggtggaat cttaattggt
    45961 ctgatcttaa attatcttct accaatgttc atcaaccagt cagaaaatat tttcgttttc
    46021 gtatacagct caagtatttt gcctggtatg gttccttggg tagtgatttt gatcagcgag
    46081 attcgcttca aaaagaagca cgcagataaa atgcatgatc atccttttaa aatgccacta
    46141 tctccttata cgaattatgt gacacttgct tttttagcta tcgtattgat tttcatgttt
    46201 atcaatcctg aaacgagaat ctcattgatt attggactcg ttttcttgct ttacatgacg
    46261 atccattact ttgtacgcga aaaaagaacc ggaaaataaa aaaagaacga agcttcacct
    46321 tagtggaagc ttcgttcttt tttattgctt ttatcaattc aattttagcg tctcaccggg
    46381 agtcaggaaa taattctcca tgctcatccc attcagcgac aagatcgttt ctactggtac
    46441 gccagttcgt tcggctattt gatttggacc ttctccttcc aaaactacgg ttgtcgcagc
    46501 ctgtgaagtc gcgctgttgg tattagtttc agaggaatct gttgtgtctt gtgttgttcg
    46561 agcttgcatt tcagaagctt ctaccgactc tgctgtctca gaatcagaag atgttgtgtc
    46621 ttgtgtgctt gttgactctg cttgaagaga ggcgctagtc tgcaaataaa tatcggaaat
    46681 cttagcgaca tgattcgtat aattagccga taattgattg atcaaagtat ctaagtctac
    46741 accgtcttta ttttctgctg cctgatacgc tgcacgtaat tttctgatcc cttcgtcgga
    46801 gattgcttga agctcacgtg ctttttggtt agcaactgct gataaaccag ctattccctc
    46861 ttgatttccc tgaatttctg tctggtattc ttcaatcaat gcaggcgtct tttcttcgat
    46921 ctttttcgaa taatcattta cgattgcttg aagttgctct ggcaaaccat ttgctgtttt
    46981 ttcttcagaa gtttgtgtct cttttgtttt agttgatgac tcacttgttt gtttcgtctc
    47041 tgttttcttt gtcgttgttt ccgttatggt cgtacttttc gcttctgacg aattttttcc
    47101 atgaaaatat agacccgcaa ctcctcctgc tatgatcaaa aacacggcaa ctgctgcccc
    47161 aatcatgatc atccgcttac gagctgcctg tttttgctgt cttcttcggg tcatccgaac
    47221 attttctccc cactcatttt catcatacat ttttttcacc accaattttt atgtataata
    47281 gcgttatcaa acgcttcata ttcttattgt agcaaataat tgtcgctgtg cacattttta
    47341 tctttgttat ttataagaaa atttcaccag ttgagatgat tccaagattg tccgtcgatt
    47401 tctccttgca gctgcattgt tcgttcgtct atctcagcca tcagtttctt tatttcatat
    47461 aagagcgcac ggtcttgata ttctgatttt tcgcttaatt catctaattg ttggatcagc
    47521 caatcatttg tcataatgaa gttcctttca tttcttgcag tgtaaaggtc aagtcttcga
    47581 tctgctcttt tgcctcagtc agtgcttcta cgatatatgc ttgtttttca gattcttctt
    47641 cctgcatttt ctgattttcc tgtactcttt gaataaacca gtcaatccgc tgagtcagcg
    47701 atggtttgcc ttccaatgag agatactgct gataagcaat tagcttctcc caacgttttt
    47761 cctgggataa atacataaat tgtgctaaaa cgaatggcgg aagataggtc atatggaatt
    47821 tatttgcgat tcgctgatac ggcctcaaaa attcggaaat cgtatattct tcttttcctc
    47881 cagcttggta ttctttttcc gccacacctg ttgtcaatac gattcctagt tctttccctt
    47941 ttaatacatg tgctgctcgt tccaagacat tatcttgcca aatttttaaa tgagcaggag
    48001 aactatacca gtaaaacgga aactggaaaa tgatccgatc atgctgggcc aagagctgta
    48061 gttcagtctc tatatctatt tttccgtctg gataacttga ttccaaatga tgccaagtag
    48121 ctggtaaacc agcagaagct tctttgaaaa attgctgatt taaagattct tcgatcgttg
    48181 gatggctaat gatgataagt gtacgcatcc cttttcctcc tttttagtct tcgctttaca
    48241 gtgtagcata accgccacaa gtttcttttg ccgatgcttg tcattttaac cagactaatc
    48301 aggttgtcca agaaactgcg gtttgttttt ccaactgttt tttcaaggta tccaaagcaa
    48361 tattcttaaa attttcgttt ttcatacgtt tacgatagtt cgtagccgtt tctccggtac
    48421 tctttttgaa cattcggcta aatacgacta gactattgaa ccctacttga taggcaatac
    48481 tttccacgga ttcatccgtc agagaaagaa gaaacgcccc tcgattgatc cgtaccccga
    48541 tcaaatattc cttgatactg gtacccatat gatttttgaa tactcgcgac aaatggctcc
    48601 gattgaccgc taaatgctgg gcgatttcct caacagacaa tttttcagca taatgtgcct
    48661 cgatgtattt gactgtttca acagctaaag aggaatgaac cttttgtaca ccaccgctga
    48721 cctttccgcc atcttttaaa aggactgata aaaaacggta ggttaatgct gttaactgta
    48781 attcattttc caaggtttct tccgtatctt tcatgcattc taaaataatg tcgatatatt
    48841 tttgtatgtc agaagagacc atcgtgtatt ggtcttctct gaaaagcgac tgccgaatga
    48901 tttcttctgc agcactccca ccaaatgaaa gccagcaata catccacgga tcttctccat
    48961 ctgataagta aaacgtcgag tctcctggac gaatcaagaa cagatcacct tttttcaatt
    49021 ggaattgttg atttttcaca tgataagatc ctttgccttc catcacgaca tgcaggatgt
    49081 aatcattgcg aatagcagga ccaaaagaat gaaacggcat cgttttagag aaaccacaaa
    49141 aatcaaaata caattctttt tctacttgtt gatgttcctt ttttaaaaaa atcgattgct
    49201 ccacgtctat ccctccttac tcctttagta taatgcaaca aaaagttaaa taaaagtcac
    49261 agaaatatat taaaaaggaa gcgatttctt gttaagttta agtcattaaa agatctttgt
    49321 tattcgttgt aaccggttta cttagtatta tatattgaac aggagaggaa tacagattat
    49381 gaaaagcaaa atgatccaac gtgtgtccta cgctttaggc gcttttggac acgacacatt
    49441 ctatgttaca ttaagcactt attttatggt attcgtgaca agccagatgt tcgcaggaac
    49501 agataaggca acgaatgcaa aaatgattgc tacagttaca agcttagttg taggtatccg
    49561 cttagttgaa attatttttg atcctttgat tggtggcatc atcgataata ctcgtacaaa
    49621 atacggtaaa ttcaaaccat ggttagttat cggcggcgtt gttagttccg tactcttggt
    49681 agcaatcttt accaatttct ttggcttagc tactcataat acgactttat ttatcatatt
    49741 atttgcaatc gttttcatct tattggatag tttttattca tttaaggata tcgctttttg
    49801 gtcaatgatt ccagctttga gtacggaaac aaaggaacgt gaaaaatttg ctacgtttgc
    49861 tcgtttcggc tcttctctag gtgcaaatgg tactactcta gtcgttgtcc ctatcgttac
    49921 tttctttacc tatcttgtta ctcataaaca tattgaaggc gcttcaggct ggttctggtt
    49981 cggtgtgatt gctgctgtta tttcaggcgg tactgcgatt atcactgcct tgggcacaaa
    50041 agaagtcgat actgcgatcc gtcagcaaaa tgaaaaagct gggatcaaag atgtgttcaa
    50101 agcagttgct caaaatgatc agttgatgtg gcttgccctt gcttatatcg cttatgcgat
    50161 tgccaatgta gcaacaactg ccgtattatt ctacttcttc aaatatgtac ttggccaacc
    50221 aactgaattt tggatcgttg gtctagttgc tgccttactt ggtttagtag ctgttccact
    50281 attcccaatg cttacacgcg tcatctctcg tcgctacgtt taccttggag ggatcgcaat
    50341 gatgatttct tcttactttt tctttacgat cgcaggttca agtttgccaa tcgttatcat
    50401 cgggctagta ttgttctact tccctcaaca attgattttc ttatccgctt tgatgacgat
    50461 cacagactct gttgaatatg gtcaatggaa aaatggtatc cgtaacgaag ctgttactct
    50521 atcaattcgc cctttactag ataaaatcgc tggtgctttg tctaacggga tcgtagggtt
    50581 tgtcgctatc gcttctggga tgactggtaa tgctactgct gctgatatca ctgctcatgg
    50641 cgtaacgact ttcaaagctt atgcattcta tgctcctgct gctttgatga ttttctccgc
    50701 tattattttt gcttggaaga ttacattgac agagaaaaaa catgcacaaa tcgttgagaa
    50761 attggaaaac aaattggctc cagctgatac aatggaagat gaacttcacg atgcagtagt
    50821 tccttcccat taagaaagga tcttgaaaat gaagattaca gaaaaagatt ttggccaagg
    50881 ttatcacttg ataaccttgg agaacaaaaa caagctagca ctttctatct ctgatttagg
    50941 tgctagaatc gtcagtttaa aatcaaatga tcgagaatta gttttgggat ttgacaccgc
    51001 tgaagaatat atcgaaaaag atccgtatat cggtgcttct atcggtcgca ccgcagggag
    51061 aatcgaaaat ggccgctttt ctctcaatgg aaaaacttac caattagcca cagatccaaa
    51121 aactggacat aatctgcatg gtggtgctcc tggatttgaa ttaaaaaaat ggtcttatgt
    51181 cattttgaat ggcgaaaatg aagcttctgt catttttact actacaagtc ccgacggaga
    51241 acatggtttt ccaggtacaa tggatgtgga aatccgctat actttgacaa aagataatat
    51301 ctggcgagtg acctctcgcg gcaccagtga ccaagatact ttattcaatc cgacaaatca
    51361 tgtttacttc aacttgacgg gagatgcttc tcagtcaatc gatcaacatg agctttggct
    51421 caatagcgaa gcttatgctc ctttgcgaac agatagtatt cctatagggg taaaagaaaa
    51481 tgctgcagga agtgctttcg attttcaaat acctaaaaaa ttagcgtccg tttttgctag
    51541 tgatttggat caaaaaaatc ttgttgacgg aatcgatcat ccatttttct taaaagaaac
    51601 cggcttgggt aaagaagccg ctcgcttgac tagccccgat aaaagaatcc aagtagatat
    51661 tgctacagat gcttctagtg tggttatttt cactgcaaat ttcggcacag aaacaccaga
    51721 aatgcgcaac cgaaaactgg cacatcacgg tggcatcaca tttgagactc aaacagctcc
    51781 aggtgcagag cgcttctcag catttggttc gattcattta aaagccggat cagtatttga
    51841 aacagtaaca gaattcaaaa tcaaaacaag aaaggaataa ctcatcatgg aaatcaaaag
    51901 aagcttaaac acaaaatttg ccgaaacatt cggacctaaa aaaactacac aatacttctc
    51961 accaggacgc atcaatttga ttggtgaaca cacagattac aatggaggcc atgtcttccc
    52021 cgcttccatc acttatggaa cgcaaggtgt agcagcacct cgagacgata ataaaatatt
    52081 agtttattcc acaaactttg acgatatcgg tgtcattagt tttactttag atgagctaga
    52141 atatgataaa aaagcaaact gggcaaatta cgtaaaagga atggtcctta aattaaaaga
    52201 agctggacac tcaatcgatc atgggtttga gttattagtg gaaggaacaa tccctaatgg
    52261 tgcaggactt tccagtagcg cttcattaga attattagta ggtgttgtat tagaagatct
    52321 gtttgattta aaaacagatc gcttggaact agtccaaaca ggtaaaaaag tagaaaatga
    52381 attcatcggt gtcaattcag gtatcatgga tcagtttgcc atcggcttcg gtgagctaga
    52441 tcatgcaatc ttattagata caaatacgct gaaatacgaa atggttccag tgaaactaga
    52501 tggttatgca atcgtcatca tgaatacgaa taagcggcgg gaattggcag attcaaaata
    52561 caatgaacgc cgtagcgaat gcgaagaagc cttggcacgt ttgcaaaaaa ctttgcctat
    52621 ctcttcttta ggagacttag atgaagaaga atttgaatca actattgacc aaatcggcga
    52681 tgatacattg atacgcagag ctcgccatgc cgtttctgaa aatcagcgga ctttgaaggc
    52741 aaaagctgaa ttagaagctg ggaacttaga agcatttggt caactgttaa atgattctca
    52801 ccactctctt cgctatgatt atgaagtaac tgggatcgaa ttagatacac tagtagatgc
    52861 tgctcaaaaa caagaaggtg tcttaggtgc acgaatgaca ggagctggat ttggtggctg
    52921 tgcgattgct cttgtcaaag aagaaaatat acctgccttc aaaaacaatg tctatgatga
    52981 atacatgaaa gttgtcggct atgctccgga attttatgtt gcacacattg gaaatgggac
    53041 aacaaagatc gatccagaag ctgttcatgt ttgataaaaa aatcaacgat ctcagctaaa
    53101 aacaaataga taaaaataac ctctcagcaa tttccaaatg tgagcctttt gctcgtattt
    53161 gaaaattgct gttttttatt gacacggaaa gagaacgtgt gttctaattg cgttgaagag
    53221 gtgattgtta tgtgggcgaa tcaaaatccg gtatttgttt atgaaaaaga accttcaaga
    53281 gatattttat gtattgactg caaatctttt tacgcctccg tagagtgcgt tgaaaaggat
    53341 cttgatcccc ttacaactaa attagtagtc atgtcctatc cgtccgattc gacagaaaca
    53401 cggggtagcg gattaatctt ggcttccagt cctgcagcaa aaaaagctta cggcatcacc
    53461 aatatcagcc gtgccagaga tcttcccttc ccctatccaa gtgatttata tattgttgcc
    53521 ccaaggatgg cctattatat ggaaaagaac agagaaatca attctatcta taaaaaatat
    53581 gcagacgaag cgaaccatca tgtctattcc gtcgatgaaa gcttccttga tgtaacaaat
    53641 agcatggcac tctttggtaa aacaaatgcg tacgacatgg ctaaactgat ccataaagat
    53701 gtgtttgagc agaccggaat ctacacgact ataggaattg gcgataaccc gctacttgct
    53761 aaactagcct tagacaatgg ctcaaaagac ggaaagcaaa tgtttgctga atggcgttat
    53821 gaagatgtac cagaaaagtt atggaaaatc tcagatatca ctgacttttg gggaatcggc
    53881 aaacgaacag caaaacgtct gaaacaaatg ggtatacatt ctgtttatga tttagctcac
    53941 gcggattatt atcacttgaa aagcaagcta ggggtcatcg gtactcagct ttatgcccat
    54001 gcttggggca tcgatcgaag ttttttggga caagaatata caccaaaatc aaaaagtatc
    54061 ggaaatagtc aaatcctaga tagagattat acaagacgag aagaaatcga gatcgttatc
    54121 aaagaaatgg ccgaccaagt cggcactcgc ttgagacgat ctgcagcaaa aacacaagta
    54181 gtcagcttat gggtaggata ctctttaggc tatcttgatc caaatggcaa gaccgggttt
    54241 caccagcaaa tgaaaatcga agcgacaaat gctagcagag atctagcaga tgctttattg
    54301 accattttcc accagcatta ccactaccag gatattcggc gtgtaggcgt gaactgttcc
    54361 gacctcgtat acgcgacagg tttacagctt aacctttttg aagatcctga aaagcagata
    54421 aatgaagcga aagttgattt tgttgtcgat acgattagaa aaaaatacgg ctttcaatca
    54481 atcatccatg cgcatagtct cttgcaagga ggacgcgcca tcgcacgtag ttctctcgtt
    54541 ggcgggcatg caggtggtat gagtggatta gaaggtggaa aagatgagac ggacagcaaa
    54601 aaaattcatt gactataacg agtatcgaga tcggccattt ggattaaaat gggggactgc
    54661 ttttgcgatg gaagaactag tcaacggtat caaaaaaatg aagaagaagc acgcaaacag
    54721 cctcttccta aacaacaaat gacattgcaa gaaatcgata gtatactgac agaagccttt
    54781 ttatctcgta aagcagtaat cattcagctc aatttaaaag atgaatttgg aagactcttg
    54841 gatgaggtcg aagggcactt cattggtgaa gcctatgaag attactttat gattgaccat
    54901 cagccgatat tgtgggaaga cgtacggtat attgagttaa aggaaacaaa aaaatggttt
    54961 gtgacatgag aggttgtgac agaagtgatc aacttcaaaa aataagaaag gatttttcga
    55021 aaattgcttc ttaaattttt gtggaatttt ggcttatttt tcgaagaagc aacttctgga
    55081 acaacgtata ttagatatca gaggttgtga aaaagtcgct tagttttgag taacaagcca
    55141 aggcgctgat ttcacaacct ctgtctatct atccatctat tcggtaagat tcatgcatga
    55201 cgtcttcttt atttagtatg tagagccctt tttcttctga ccattcaaca taaatcagct
    55261 gttttacatc tgcatagcct tctttcttca agttctccat aagccatgct tcagtcaatt
    55321 gattggactg aagcacttta tgctgaattt gcccttcatc tacaagcagg atcgataaag
    55381 ctttgtcttc atctgatttt ttcaatacac tcatttgacc ggcattctct aggataacat
    55441 gtttggcatt ttcaagaaaa aagcattctt gctgtcttaa gattgctcgc aactgctcca
    55501 tctcgatatg gttatttgtc aactctgtca tgttgatgac gccgtctttg attagaacag
    55561 atggctcgcc tttgacccaa cgattgacct tttcattttt ttggacgata cgttcgatac
    55621 cataaatcat tactgcccaa agcgcaatgg caaataggac atgccctaca tggacattgt
    55681 catcataggt tgattcttct aagatcccac ctaaaacaag cgtatagact aaatcaaacg
    55741 gtgtgatttc tgacatggat ttcttcccaa gcaaacgtac cactaataat aaaccgatca
    55801 acccgaacac cagtttcatt gtaattgaaa aaatcatttt ttctccccct aactatctgt
    55861 taattaaatt atatatgctt tctcacgaac acgcatggaa tatacaagga agaaatgtat
    55921 ccttttacta aaaaaaggac tgtgacactg tagtgacccc caaaagttag acttttttgg
    55981 agtggagaaa tccactcctt ttttttatgc tctaaaatga tatttttaca caaccgttac
    56041 ggttgtttta cgaaattgga cg
//
LOCUS       DAFGZE010000010        54519 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.010, whole genome shotgun sequence.
ACCESSION   DAFGZE010000010 DAFGZE010000000
VERSION     DAFGZE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.010"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..360)
                     /locus_tag="LTU54_000608"
     CDS             complement(<1..360)
                     /locus_tag="LTU54_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295345.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGI"
     gene            complement(657..3038)
                     /locus_tag="LTU54_000609"
     CDS             complement(657..3038)
                     /locus_tag="LTU54_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HBL8295346.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3028..3675)
                     /gene="recU"
                     /locus_tag="LTU54_000610"
     CDS             complement(3028..3675)
                     /gene="recU"
                     /locus_tag="LTU54_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HBL8295347.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3739..4278
                     /locus_tag="LTU54_000611"
     CDS             3739..4278
                     /locus_tag="LTU54_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HBL8295348.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4368..4787
                     /gene="gpsB"
                     /locus_tag="LTU54_000612"
     CDS             4368..4787
                     /gene="gpsB"
                     /locus_tag="LTU54_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HBL8295349.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4809..5176
                     /gene="rnpB"
                     /locus_tag="LTU54_000613"
     ncRNA           4809..5176
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="LTU54_000613"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5385..6551
                     /locus_tag="LTU54_000614"
     CDS             5385..6551
                     /locus_tag="LTU54_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBL8295350.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6597..8093
                     /locus_tag="LTU54_000615"
     CDS             6597..8093
                     /locus_tag="LTU54_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HBL8295351.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8174..8857
                     /locus_tag="LTU54_000616"
     CDS             8174..8857
                     /locus_tag="LTU54_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL8295352.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9502..10692
                     /locus_tag="LTU54_000617"
     CDS             9502..10692
                     /locus_tag="LTU54_000617"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBL8295353.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10794..11747
                     /locus_tag="LTU54_000618"
     CDS             10794..11747
                     /locus_tag="LTU54_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBL8295354.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSGNVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11816..13165
                     /locus_tag="LTU54_000619"
     CDS             11816..13165
                     /locus_tag="LTU54_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8295355.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13348..13671)
                     /locus_tag="LTU54_000620"
     CDS             complement(13348..13671)
                     /locus_tag="LTU54_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295356.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13800..14963
                     /locus_tag="LTU54_000621"
     CDS             13800..14963
                     /locus_tag="LTU54_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL8295357.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15033..16382
                     /locus_tag="LTU54_000622"
     CDS             15033..16382
                     /locus_tag="LTU54_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL8295358.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16427..16858)
                     /locus_tag="LTU54_000623"
     CDS             complement(16427..16858)
                     /locus_tag="LTU54_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295359.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            17246..19888
                     /gene="alaS"
                     /locus_tag="LTU54_000624"
     CDS             17246..19888
                     /gene="alaS"
                     /locus_tag="LTU54_000624"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBL8295360.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKEHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            20088..20684
                     /locus_tag="LTU54_000625"
     CDS             20088..20684
                     /locus_tag="LTU54_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL8295361.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20806..21954
                     /locus_tag="LTU54_000626"
     CDS             20806..21954
                     /locus_tag="LTU54_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL8295362.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21992..22405)
                     /locus_tag="LTU54_000627"
     CDS             complement(21992..22405)
                     /locus_tag="LTU54_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HBL8295363.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22484..22915
                     /locus_tag="LTU54_000628"
     CDS             22484..22915
                     /locus_tag="LTU54_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HBL8295364.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(23014..23247)
                     /locus_tag="LTU54_000629"
     CDS             complement(23014..23247)
                     /locus_tag="LTU54_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HBL8295365.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            23430..25100
                     /locus_tag="LTU54_000630"
     CDS             23430..25100
                     /locus_tag="LTU54_000630"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBL8295366.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            25247..25633
                     /locus_tag="LTU54_000631"
     CDS             25247..25633
                     /locus_tag="LTU54_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295367.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25620..26318
                     /locus_tag="LTU54_000632"
     CDS             25620..26318
                     /locus_tag="LTU54_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL8295368.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            26323..26787
                     /locus_tag="LTU54_000633"
     CDS             26323..26787
                     /locus_tag="LTU54_000633"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBL8295369.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26784..27695
                     /locus_tag="LTU54_000634"
     CDS             26784..27695
                     /locus_tag="LTU54_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL8295370.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27930..28469
                     /gene="pyrR"
                     /locus_tag="LTU54_000635"
     CDS             27930..28469
                     /gene="pyrR"
                     /locus_tag="LTU54_000635"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBL8295371.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28483..29766
                     /locus_tag="LTU54_000636"
     CDS             28483..29766
                     /locus_tag="LTU54_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBL8295372.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29779..30705
                     /locus_tag="LTU54_000637"
     CDS             29779..30705
                     /locus_tag="LTU54_000637"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HBL8295373.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30724..32004
                     /locus_tag="LTU54_000638"
     CDS             30724..32004
                     /locus_tag="LTU54_000638"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBL8295374.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            32009..33085
                     /gene="carA"
                     /locus_tag="LTU54_000639"
     CDS             32009..33085
                     /gene="carA"
                     /locus_tag="LTU54_000639"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBL8295375.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            33090..36275
                     /gene="carB"
                     /locus_tag="LTU54_000640"
     CDS             33090..36275
                     /gene="carB"
                     /locus_tag="LTU54_000640"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBL8295376.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            36329..37105
                     /locus_tag="LTU54_000641"
     CDS             36329..37105
                     /locus_tag="LTU54_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBL8295377.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            37102..38034
                     /locus_tag="LTU54_000642"
     CDS             37102..38034
                     /locus_tag="LTU54_000642"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBL8295378.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            38031..38747
                     /gene="pyrF"
                     /locus_tag="LTU54_000643"
     CDS             38031..38747
                     /gene="pyrF"
                     /locus_tag="LTU54_000643"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBL8295379.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38754..39383
                     /locus_tag="LTU54_000644"
     CDS             38754..39383
                     /locus_tag="LTU54_000644"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBL8295380.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            39371..39685
                     /locus_tag="LTU54_000645"
     CDS             39371..39685
                     /locus_tag="LTU54_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295381.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            39723..40364
                     /locus_tag="LTU54_000646"
     CDS             39723..40364
                     /locus_tag="LTU54_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL8295382.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            40407..41099
                     /locus_tag="LTU54_000647"
     CDS             40407..41099
                     /locus_tag="LTU54_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HBL8295383.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            41096..41998
                     /locus_tag="LTU54_000648"
     CDS             41096..41998
                     /locus_tag="LTU54_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8295384.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(42156..43862)
                     /gene="efbA"
                     /locus_tag="LTU54_000649"
     CDS             complement(42156..43862)
                     /gene="efbA"
                     /locus_tag="LTU54_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HBL8295385.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            44052..44675
                     /locus_tag="LTU54_000650"
     CDS             44052..44675
                     /locus_tag="LTU54_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295386.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            45125..46174
                     /locus_tag="LTU54_000651"
     CDS             45125..46174
                     /locus_tag="LTU54_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8295387.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            46171..47052
                     /locus_tag="LTU54_000652"
     CDS             46171..47052
                     /locus_tag="LTU54_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295388.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            47055..47825
                     /locus_tag="LTU54_000653"
     CDS             47055..47825
                     /locus_tag="LTU54_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8295389.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            48104..48958
                     /gene="aroE"
                     /locus_tag="LTU54_000654"
     CDS             48104..48958
                     /gene="aroE"
                     /locus_tag="LTU54_000654"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBL8295390.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            49136..50299
                     /locus_tag="LTU54_000655"
     CDS             49136..50299
                     /locus_tag="LTU54_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL8295391.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            50416..50826
                     /locus_tag="LTU54_000656"
     CDS             50416..50826
                     /locus_tag="LTU54_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8295392.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            51000..53228
                     /gene="topB"
                     /locus_tag="LTU54_000657"
     CDS             51000..53228
                     /gene="topB"
                     /locus_tag="LTU54_000657"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL8295393.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            53399..53683
                     /locus_tag="LTU54_000658"
     CDS             53399..53683
                     /locus_tag="LTU54_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295394.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            53843..54079
                     /locus_tag="LTU54_000659"
     CDS             53843..54079
                     /locus_tag="LTU54_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL8295395.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            54076..54475
                     /locus_tag="LTU54_000660"
                     /pseudo
     CDS             54076..54475
                     /locus_tag="LTU54_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
BASE COUNT        17800 a         9504 c        11763 g        15452 t
ORIGIN      
        1 aataccattt ataactaaac ataaactaat ggatttgggc ggtttcaggc tcatcagaaa
       61 aatatagatt ctggtgaaca acaaccataa aaaagtgaga atcattgcat gagagcttct
      121 ttccataaat tttcctttaa gagtagtgct taaaagtaca gaaaaaataa taaatgcaag
      181 gataggagca attaaagtaa atttatttaa taataaacaa aatttttcag cttttttatt
      241 cttggaataa cgaggtattg ttttcgataa aaaaagatgg tgcagacaaa ttatgataat
      301 taagcatact atcagataac tcaaaatgaa gccttctttc aaaaaattat tcaaaaccat
      361 atattgtatt ttttatttga taataacaat gggattatta agaagtggca ctaaataaaa
      421 gactagtgtg tatttagccg atttaattat aaacaagtaa atggataaag tatatgtaat
      481 gataaaagaa aaatggattt tctaattttt aagctaaatg ttagatcaca catgtatatg
      541 ctggatagtc ttataaaaaa tcatgtaata cagcttggta catgattttt atttgtagct
      601 gttttttaca tagacacaaa aaagaaacca cgcttgtaga cgtggtttct tttttactat
      661 ctgtttccag aagctgaggt gctgctggat ggtgtatggt catttgcagc atccgctccg
      721 ctagttgctg gctcactgga agaagcagga ggagcagatg aagacggtgt cgagctttct
      781 tcggatgaag aagaaggaac cgttgttgaa gacgctggct cgcttgtttc tgctgtcgtg
      841 ctttcttttg aggactcagc agttgattcg ctttgactag tagagctaga gctttgagtc
      901 gttgtctctg ttgttgagct tccaggagtc tggacatagc tggaagatgg aatagttgtt
      961 gatggtgtaa ttgcattcga tctagctgta tattgatctt tgtagtacag ttcaccgccc
     1021 acacgaatta atccgcttgg catctcccag tctgtatttg tcacgttagc tgaaacatac
     1081 tgcataagct cgcgataaac atcagaggct acgtgtgacg attctgaagt gacaggtgtc
     1141 atttttttat tgtaacctgt ccacacggaa atcgaataat ttggcgtata gcccgcaaat
     1201 aggatatctg gatacacccc agaagaaata ccaagttttg catactcgtc atctgtgtaa
     1261 ttggatgttc cggtcttacc ggcttgatat aacccagcga tttgcgcatt cgtaccagtt
     1321 ccttcagtga tcgtatcttt taaaatgtct gtgatcatat aagctgtttc tggagacatg
     1381 gctgtttttc catctggctc ataagtttct tctgttccgt cttggaagac gattttatta
     1441 acatattgag gtttgtaata agtccctcca ttggcgaaag ccgcataggc tgctgccatt
     1501 tttaaggaag aagctccgta ttttgtccca tcttgttctt ccgtattact agagatcgca
     1561 ttcgattgat ggatcgtact gtattcgatc ccaagatttt ttaagaaaga agccacttta
     1621 tctgaaccga cttcattgaa taatttgact gctggaacat tacgggataa gtaaagtgct
     1681 tggcgtaaag taatggtgcc catatattga ttatcccagt ttcctacagg agtcgaagtc
     1741 ccttcatagt tatacggtgc gtcagtaata gtttttccag tagaatattt caaatattca
     1801 aaagctggtc cgtaatctgt gacaggtttc atcgtagaac caaaatcacg ggaagtattc
     1861 actgctaagt tgttgcctaa agttacgtct tctgcgatgt gacgtccgcc aatctgggct
     1921 tttactttcc cggtattggt gtcgataagg gtggaggcaa cttgcatctc gtcgtctggg
     1981 taagagacat actgatccgt gttgacgata tcatatagtt tcttttgggc gtccagatcc
     2041 aaattcgtat aaatctctaa accgtctgtg tagacattct tatctgtttt ttcttgtact
     2101 tcattgatga cttctttgac ataattatcg acgatcttag tattgtcatc ggattgagtt
     2161 agttcttgca gaccatccgt taccggtaca ttaacagctt gatcatattc tgtttgagag
     2221 attttttcgt tttgaagcat cgtatacaaa acagtatctc gtcttttctt cgcctgatct
     2281 ggataaacat aaggatcata ggcggaaggg gcttgaggca tcccagcaag caatgctgtt
     2341 tgcggtaaag aaagttctga taatttctta ccaaagtaca tctcagaagc tgtctccatc
     2401 ccgtacaagc cgtttgacat atacacttta tttacgtagt aggtcaagat ctcttgtttc
     2461 gatttttttt gttccaatct tacagccatc cacgcttctt gggccttgcg tttcagagtc
     2521 tgatcctcgg cactggtgga gaagaaggaa agtttgatca attgctgtgt caaagtactt
     2581 ccgccttgca gtccgccaga ggtaaagtta gagagggcag aaccgatgat ccgaataggg
     2641 tcaaccccga tgtgtttata aaaacggcga tcttctaccg acacgatcgc atcttctaat
     2701 gttttcggta attcattcgc agaaattttt tcacgctttt ctgcgcctaa atcttcgaat
     2761 agttcgccat cttgtgtata tagtttggaa gatacggtag cgtctaattt tttatccgtc
     2821 aattcgggcg catcttttgc gtaataccag aaaagtccta cacctgctaa aaagaggata
     2881 cagaaaaatg agagtattcc taaaaggatc ttgataaata atcctttttt atgtgttcca
     2941 gaggactttc cagaaccttt gccagaattc ttcttcacac tctttttagg agtaggctgt
     3001 ttctgtcgtc tagagcttct agtttgttca tttgccattc ttgctttctc cttcaataga
     3061 taatgttgat aaatagaagt caaccgcgtc caaataagga atccgcggag aaataccagt
     3121 tttgatctct attcctagtt cttcgatcaa agataaaggt agtgatttgc gtcctttttc
     3181 cttttgggcg tcccagtaag caatcaaatg aacactattc aaataaaaac agcgcttcaa
     3241 agacgaaaac cataacaaga caaagcagac cccattttgt tcgaggcatg ctttcatatg
     3301 ttcgatttga tgggcatgga agtttttaaa gggaaaagat gttttgttct tggtttcttt
     3361 tgcttcaaag tccaagtaaa agcctcgata gacgccatta tagtctgtag ttgaggcttg
     3421 ggtaaagtag gcttccttga tcacagcagc acttctctta ggatagtcta ctttcacgat
     3481 ttggataggg gtaggctttt tatggataac cgccaacccg cgggaaagat aatatttatt
     3541 actttcattg attgcttctt caaagcgcat cccccgattg ccgaattcag tgatctgatt
     3601 tcttttttct tgaatttttt cgacgggaga ggcattctta taataaggtt ggccatttgg
     3661 atagcgcaat accatcttta atcacactcc taggccacat tatagcaaaa aagcggatag
     3721 aaagaaaagg aatctgagat ggataagtca aaagtaatct atgtgtctgg gtatcgaagt
     3781 ttcgaactcg gtgtttttaa agaaaatgat cctaaaatcc aagttgttaa aaatgtttta
     3841 aaaaaagaac tccatcagtt agcagaagaa gggctggaat gggtacttgt cagtgggaat
     3901 ttgggcgtcg aactatgggc tgtgcaaata gtagcggaat tgaaagaaga atatccggaa
     3961 ctatctcttg gtattttgta tcctttttct gattttggta ataactggaa tgaagggaac
     4021 caagaaaaaa aacaattagc agaacaatta gcagattatg tcgaagctgt tagccatcaa
     4081 ccctaccaat cacctagtca gctaaagaat cacactcgtt ttatacttga gcatacagtg
     4141 ggctgtctgc tgatctatga tgaagaattc cctggaaaaa cgaagtattt ttttgaagat
     4201 gcacagaatt accaagcaga tcatccctac gaaattcggc tgatcagtat ggacgatctt
     4261 caaaatttta gtgaataatc gatattgtgt ttgatttatc aacttttttc ctatacaatg
     4321 aaaaaggtag aaatgttatt acgtaaatga aaactagagg tgtaaatatg gcgaatttgg
     4381 tttatagtcc taaagacatt ttacaacaag aattcaaaac aaaaatgcgc ggctatgatc
     4441 ccgttgaagt tgatgaattc ttagacaata tcatcaaaga ttatgaaact tacagcaaag
     4501 aactccttgc tttacaagaa gaaaacgatc gattgagtgc aaaagtagct caattatcta
     4561 aaactcaagg agcagctcaa acacgcgtgc aacaaacaga ggcaccaaag agcgcagcag
     4621 tgaccaattt tgatatctta aaacgcttgt caaatcttga acgtgaagta tttggtaaaa
     4681 agcttgatca acaagcaagt gcagtcaaac cagcacaacc taatccaaat aactacacaa
     4741 atgcagacac aagcttagat gataatgaaa aaacacgtca attttaattt ttgattaatg
     4801 aatttgcggt tttcgggtaa tcgcggtctg acttgatcag gctgaggaaa gtccatgctc
     4861 gcacaggctg tgatgcctgt agtgttcgtg cttagcgaaa ccataagcta aggtactctt
     4921 tggagtaacg gcaggaaaac aagctaaggc ttcggctatg cttaagtatc cttgaaagtg
     4981 ccacagtgac gaagcggctg gggaaaccca tccggtggaa cgcggtaaac ccctcgagcg
     5041 agcaacccaa acaatggtag gggcgctttt tgtctggaag aagaacagga caaaaaggca
     5101 gtataactgc agatagatgg ttaccgcttt gttttttctc cctggaaaag caaagtacag
     5161 aacatggctt atagaaaacc gcaaacatca gggaattagg tcgtgacaaa agtaatgcgt
     5221 tataagaaac aagaaaaaca tttgaaagct atttttcaat acttcagatg aatcgttctt
     5281 attacaaaac aaacttttgt atcaccggtt attcggaatt tgagtaaaag tgtggggtag
     5341 gactgatgtc ccacctcctt tttttataaa taaggagatt gtgtatggaa aaaaacagga
     5401 agttcaacct cgtcgcaact gcggcaagcg ggctagaagc actggttggt aaagaattaa
     5461 gagatcttgg aatcgaatgc caagttgaga acggacgtgc acgttttgaa ggaacaatgg
     5521 aaacgatcgc tacagctaat ttatggttac gtacagcaga tcgagtaaaa atcgtggttg
     5581 gggaattcga tgcgtatacg tttgatgaat tgtttgaaaa cgtaaaagca ttgccttggg
     5641 aagattttct tccactagat gcagcttttc ctgtagctgg aaaatcgatc aagtcgaaac
     5701 tatatagcac acctgattgc caagcgatca caaaaaaagc aatcgttgaa cgtctccgta
     5761 cctattatca ccgtccagct tctgtaccac tgacggaaac tggcgcacat tttcagttgg
     5821 aagtagcttt gctgaaagac catgtgatgg taactctgga tacgacagga ccaagtttat
     5881 tcaaacgtgg ttatcgctta gaaaaaggtg gagcaccatt gaaagaaaac atggcagcag
     5941 cacttattat gttgacaaat tggcgaaaag accgtccatt ttatgatcct gtatgtggtt
     6001 caggtacgct ttgtattgaa gcagccttaa taggccataa tatcgcacca ggattcaatc
     6061 gtgagtttgc ttgcgaatct tgggattggt tcagtcaaga aatcatggaa aatgttcgat
     6121 cagcagccga agaaaaagca gattatgata ttgaattaga tattactggt tcagatatca
     6181 atggacggat gattgaaatt gccaaagcca atgcagaaga aatcggatta ggctcttcga
     6241 ttacctttaa gcaacaagca gtcaaagact tcaaaacgga taaagaatac ggggtcatcg
     6301 tggctaatcc gccttacgga gaacgtctag gagaagaaga aactgttcgg aaattatata
     6361 aagaaatggg cgatgtattc cgtccattaa aaacatggag taaatatatc ttgactagcg
     6421 acttagcgtt tgaggaattt tacggacaaa aagcaaccaa aaaaagaaaa ttgtacaatg
     6481 gtgcattgcg tacggatcta ttccagtatt ggggaactcg tccgcctaga aaaaaagaga
     6541 aagacgaata atcttactaa ttgattcatt caattggatg tttggaggaa tagggaatga
     6601 aagaaaaaga gtttttacaa gaagtaaaag aaatcaatct tctacaacaa gctttaggaa
     6661 tcctcgattg ggatacacaa acaggtatgc ctgaaaaagc tagtgcatac cgaagtgaag
     6721 tagacagcta tctttacagc atttattttt caaaaaaaat cggtccaaaa atcaaagaag
     6781 ccattcgcta tttttctgaa catccggaag aattgtcgga agttggaact gccgtttttg
     6841 ccaaagtaaa agaagagtat gatttggaac atcgcgtacc ggaagatctt atgagaaaat
     6901 atacagctgc cgtttcttca gcacacggac aatggcaaaa agctagagaa acacaacaat
     6961 tttctgattt tcaacacgca ttgaatcaaa atattgaatt aacaaaacag ttgatacctt
     7021 actggagaaa agatgaaaag acgaattatg atgttttgct gaatcaatac gaacccggga
     7081 tgactgtcga aatccttgat aaggtatttg atcagcttaa agaaggaatt ctttcgatcc
     7141 gccgtacgct ccaagaaaaa gggactgaac ctgaaacaga ctttttaaat cggagaatga
     7201 cgaaaaaaca acagaagaga tttgtagtga aagtaatcga acaacttggt tatgatttta
     7261 gtcgtggtcg tttagatgac actgtccacc catttatgac tggaattaat ccgaatgatg
     7321 cacgaatcac gacacgatgg aacgaaacag atttcaaaat ggctgttttt ggagtgatcc
     7381 acgaggctgg tcatggtatg tatgaacaag acatcgatga taaatatgcg tatacaccgg
     7441 tttatgaagg tacatcaatg ggtatccatg aatcacaatc gttgtttaac gagatcatta
     7501 ttggaagtag tcgcagcttc tggaaaaagc aatatccttt ctttcaagaa tgtgcagaag
     7561 gaacattcga tgatattcct tttgacaaat tttatcgttc attgaaccat acaaaatcaa
     7621 gtttggttcg tatcgaggca gacagtttga cttaccctct ccatatcatc attcgttatg
     7681 aaatcgaaaa aatgattttc aatgaagggg tagatgttgc tgaactacct gaaatatgga
     7741 atcaaaaata tgaagagtat ctgggaatac gaccaacaaa tgatgtggaa ggcatcctac
     7801 aagacgttca ttggtcaggt gcaagctttg gttattttcc ctcttatgca ttagggtaca
     7861 tgtatgcggc acaattactt cattctttgc agcaagaaat agatgtggaa aatgttctag
     7921 cgtcagacga ttattctcca atcaaaaact ggatgtcaga gcatatccat caatatggtg
     7981 catctagaaa accaaatcaa ttaatcatgg atgcaacaca tgaaccgctt aatccgcaat
     8041 acctgatcga ctatatgaaa gacctgtatt ttaatgtata tcaggtaaaa taaaatacaa
     8101 tttacaaaga tagctgaaat tcatctaatc tatagatgtt ttcagctatt tacttaagaa
     8161 agaagtggaa cgaatggata aattacaagt gatcgctgat tatagtatgg accaattagc
     8221 gcatgatcaa acaggacatg ggagcgatca taccaaaaga gttgtcaagt tagctgaaaa
     8281 aattttggag acggaaccgc aggcagaccg ctttgtgacg cttgcagcag cttacctaca
     8341 tgatacgatt gatgataaag tcgttaaaaa tgagaacgag gcaaagcaac agcttcgtgt
     8401 attccttcgc tctttgccaa tagtagagga acagatctcg atgatttttt ccattatcga
     8461 aaatatgtcc ttttctaaaa atctctctga agcggccgaa ctatctctgg aaggtaagat
     8521 cgttcaagac gctgatcgta tcgaagcatt aggagcaatc ggcattttgc gtactgctta
     8581 tttcggcggt ggacatgggc acccgatttt tgatcctgaa ctgtatccgc aaacattcaa
     8641 ggacaaagaa aattaccgaa aaggcacgac agtgatcaat catttttatg aaaaactgtt
     8701 cttgctgtct gataaaatga atactgatta cggatatgaa gaagccaaac gaagagaaac
     8761 cttcatgcgt gaattcttag aagaattttt tctggaatgg tctattgacg atgaaaattt
     8821 tactccagac aattggatca aaaataacaa aaaatgatgg aggttgtgac agaagtggtc
     8881 agctccaaga tatttcggat cattttccgt aaaagttact tctggaacac tgtttaccaa
     8941 gaggtcgtga gcctgccgtt cagctccgag tcacaaggaa caatttcaaa aaatagcttc
     9001 tcgtgtttga tgaatattgt aacttgtgat tcggaattgg caggagaaca ccgtttattc
     9061 ggaagaatag gactgtgaca agacttttgt cacagtcctt ttttcgaatt caacaagtaa
     9121 aacttttaag aaatattcag agagtaaaat taaaaattgc tgataaacga agatggaaat
     9181 tttatgtatt tttattggtc tatgcaataa ataaattaaa tattcagaat acaattgatt
     9241 ttaacgaaag ggcttctatc aatggttgtg ttgtatttat ctgcccaggt agagtaaaat
     9301 agatcattat ctaaaaggta attgttcata ctatataagt catttgaaag taaaaagata
     9361 ctgtaaataa aaaatgtggt taaataattc tcataatcag tgtaagcgtt atcaaaaata
     9421 tagcatactt gctttgtaga tgaatgacac gatatatgtt tggtatgttc tgcaagacag
     9481 atgaatatgg aggtggagac aatgtcttcc aaagaactaa caatgtattt agagaaagaa
     9541 ttggatcaac tgaaagaaaa ggggttatac aatacgatcg acatactaga aagtgaaaat
     9601 ggtgcttgta tcatcgtaga cggaaaaaag atgatcaatc tagcatccaa caattatctt
     9661 ggatttgcca atcgcgaaga attgaaaaaa gcatgtattg aagcaaccga aacttatggt
     9721 gtaggagcag gagcagtccg aacgatcaat ggttcgctga aaatccatca gcaattagaa
     9781 gaaaaaatcg cagaattcaa agggacagaa gcagcaatcg cctttcaatc tggattcaac
     9841 tgcaatatgg gcgctatctc tgcagtaatg acaaaagaag atgctatctt atcggatgaa
     9901 ttaaaccatg catctattat tgacggttgc cgcttatccg gagcaaaaat catccgaatt
     9961 aaacatcaag atatgaaaga ccttgagaaa aaagcaaagg aagcgataga aagtaagaaa
    10021 tataaaaaaa tcatgtatat cacagatggt gttttctcca tggatggaga cattgcacga
    10081 ttgccggaaa ttattccgat tgttgaaaaa tatggactga tcacctacgt cgatgatgcg
    10141 cacggctcag gcgtaactgg aaaaggtgca ggaaccgtca agcattttgg attaagcgac
    10201 aaaatcgata tgcaaatggg cacgttatcg aaagctatag gtgttgttgg gggttatgta
    10261 gctggatcga aaacactaat cgactggttg aaagctagaa gtcggccatt cctattttct
    10321 acttcgctaa ctccaggtgc agctgcagct gcattggctt cgattacttt gatgcaagaa
    10381 catccggaac ttgtcgaaaa agtttgggaa aatgctaatt attttaaaga agaattgaaa
    10441 aaagtaggct ataacatcgg tatgtctgaa acacctatta ctccagtcat tttgggagac
    10501 gaaaaagtga ctcagacttt ctctaagaag ctgatcgaac atgggattta tgctaaacca
    10561 atcgtttatc caactgtgcc tcttgggaca ggcaggatca gaaatatgcc gactgcggaa
    10621 catacgaggg aaatgcttga tgaagcagta gcagtttatc aaaaaatagg aaaagaaatg
    10681 gaaattattt aactaaattt agttttttcg tctatttaaa attagagaac atgttcacgc
    10741 atatattgtt aatcatctga agatagtagt aaaaaagatc agggaggatc atcatgaaaa
    10801 aagtattggt gacaggatgt ttaggacaaa ttggttcaga gttgatcaca cggctacgtc
    10861 aagaactagg cagtggaaat gtcattgcga cagatatacg catgcctgaa gtcattgaga
    10921 aaaacgatat ctttgagatc ctagatgtaa tggattacga aaaaatgcgc agcatagcag
    10981 aacaatatca agtggacaca ttgattcatt tggctgctct tttgtcagct gttgcagaag
    11041 caaaaccaca attagcctgg aatttgaacg tgactgggct ggtcaatgca ttggaagtcg
    11101 ctagagaatt ccagctgaaa ttttttacgc caagttcaat cggcgcgttt ggtccaaata
    11161 ctccaaaaga tcatacccca caagatactc ttcagcgtcc tacaaccatg tatggggtaa
    11221 cgaaggtagc tggagaacta ctatgtgatt attattatgt gaaatatggc gtagatactc
    11281 gtggtgtccg tttccctgga ttgatttctt atgcaacctt gccaggaggc ggtacgacag
    11341 actacgcagt cgatatttat tattcagcaa tcaaagaagg aaagtacact agtttcattg
    11401 atcaaggtac atcaatggat atgatgtata tgccagatgc gatcgatgcc attataaaac
    11461 tcatgaatgc agatcctgat cgattggttc atcgaaatgc cttcaatata tcggcgatgt
    11521 cttttgaccc ggaagagatc aaagcatcta ttcaaaaaat cataccagat tttgaaatgg
    11581 attatgatgt ggatcctgta cgtcagtcaa tagcagaatc ttggccaaat tcactagatg
    11641 tttcttgtgc gcaagaggaa tggggctttt cgccgaaata cgacttagat gtgatgacaa
    11701 aggatatgtt ggaaaaactg aaagaaaaat tggtagagac ggtataaaac aagaaccaca
    11761 tccaaaaagt tttttatact tttgggattg gctatggtat gaaaggggac aagggatgga
    11821 agaatatcaa gccacgccag taaaagaagt aaaaatagaa aatgaattaa aacgtacgat
    11881 gggatttttt actgctttat cgactgttat gggcacagtg atcggtgcag gggtattttt
    11941 taaagccgca agcgtagcag aagttacagg atccgccagt ttgcatatgt tttcttggtt
    12001 tctcggtggt atgatttctg tctgtgcagg actaacagga gctgaactgg cagctgctat
    12061 tccagaaaca ggagggatga ttaaatatat cgaacggatt tacggaaata cagcagcttt
    12121 tttattgggc tgggcgcaag tcgtgattta ttttccagca aatgtagcag ccttgtcgat
    12181 cattttcggt acacagttcg tcaatctatt tggtctttcc cagtcaatga ttgttcccgt
    12241 agctgttaca gctgctgtat cgatcctgtt gatcaatttt ttaggttcta aagctggagg
    12301 ggcttttcag tcgattacgc ttgtctgcaa gcttattcct ttgtttgtga tcgtcatttt
    12361 cggtttgttc cgacaagaag gagtagactt ccaactgttt ccgatccaag caggagaaaa
    12421 tctttcattt ttctcagcct tgggagcggg cttgctagca acgatgttcg catacgacgg
    12481 ttggatacac gtaggaaata tttcgggaga attgaaaaag ccggcgaaag atttgccaaa
    12541 agcaatttct ttaggaatca tcggaatcat gatcgtttat ttattagtaa atgcggtgtt
    12601 cttaagaacc gcatctatcg acggagtcgc aggaaacagc aatgcggcaa gtgacgttgc
    12661 aaaaatgatt tttggcggct tcggtggaag actggtcaca gttggtatct tgatttctgt
    12721 ttatggaacc atcaatggct atacccttac tggaatgcgt ctcccttatg ttatggcaaa
    12781 agaaaacaac ctgcctttta gcaagctctt tgctaaactt catgataaaa ctaaagttcc
    12841 tgtagccgca ggtatcttag agctagtcat cgctattggc atgatgatga tcggtggttt
    12901 tgatacacta acggatatgc tgatctttgt catttggatt ttttatacga tggtctttgt
    12961 tggtgtcatt cttcttcgta aaaaagaacc tgatttgttt cgaccataca aagttccaat
    13021 gtatccgttt attccactag tagcaatcat tggagggact tttatcgtat caagcacgct
    13081 tatcactcag acctttttag cttcgatggg gatagtattg acacttgctg gtatcccgat
    13141 ttatctctat ttaaaacgta gatagctaaa acattcgaat tttattagaa ctgcacttct
    13201 ctgccaaaca aaatatttta tttaaatgag gagtacgtgg aagaaatgtc aaaagtactc
    13261 gttaattttg atcacgtaaa tgtcgttttg tctacgatga aatagcaaag taaaagacag
    13321 aaatcgggct caaccgattt ctgtctttta ctttgatttg cgtaactgaa ctattcggcg
    13381 tacgccgcga taaatattta ataaaaccaa gcaaccagaa atgaaaatgg gaagccaaat
    13441 cagatagaca gggtagccaa gcactttaga caaagcatgt ggagtcattt tatccatttc
    13501 aagaagtgct tgctgccaag tctgaagatt gaagactagc atgaaacccg aaataatcag
    13561 gagaataaat cctaaataat gaattgtagt tctttttctt acataagcga tcgtagcata
    13621 aaggaacagt aaaaaaatag gtacgccaaa aacaaagtac atattcagca tctcgacacc
    13681 ccctttgtgt ttatcttact ttactttgaa agaaatgaga aataatagcc tcgttacttg
    13741 cttgaaacag caaaggactg ctaagataag agagatacaa agaatcaagg aggagtcata
    13801 tggcaagtta tatcgaaccg attcgttttg cgctgattgt ttttccattt ttagccttag
    13861 cgatctcatc tgtttttttt atttatgaat atcgaaagta tggaacattt ctagtaacaa
    13921 gggcagttat tttgtattct ttcgtttttt atttattgtg tgcgtatttt ttggtgatct
    13981 taccacttcc ggcaaaggag acggttgccc aaatgacagg gccaaagatg gagctgcatt
    14041 tatttgcttc atggcaacat ttcagagaca acacggtatt ggtcttgtct gaacctagta
    14101 cctacctgcc ggcaatgaag cagagtgtat ttttggaacc agcttttaat gttttattac
    14161 tcataccatt tggcatttat ttaagatatt atttccggct ttcttttatg aaaacgatga
    14221 ttgctagttt ctgcctttct ctattttttg aattgacgca gctaagtgga ctttatttta
    14281 tctacccaag accataccgc ttatttgatg tgaatgattt agtgacaaat acattgggtg
    14341 gaatcattgg ttttgtcgtt acgccgttat ttacatttat gcttcctaca agaagacgaa
    14401 tggatgaact ttcttatgaa aaaggaaaga ctgtctcatt gactcgacgc ttggtcgctt
    14461 atttgatcga ctggacattt ttatctctta ttaatttggt agcggcgatt ttctttaaaa
    14521 ttgcgttcga ttcaggggca gttacttctc atatctggtg gcttttatta gaagtagggg
    14581 tatactttat gattgtaccg tttataacca atggatggac acttggcaaa aaaattgtgc
    14641 gtatccgttt ggtggaagaa gaccgcgaac gaatttcttt aaaagcattg atgatccgct
    14701 acgggtatct gtactttctt ttttatggtt taagctggtt ggggcagttg cttcagcaaa
    14761 tcatgaattc gacaacgaat acggtgttgc agatgagtgc agtattcatt tctctcggtg
    14821 tcttgttact acaaggtatt tttattttaa atatcttgct atcatttttg agaaaaaaac
    14881 ggattctgtt ttatgaaaaa gctagtcaca catatactgt tagcacgatt ggccaaacaa
    14941 aggatgatga ttctcgaaat tagctgagca aaaacgcttt gtctgtgtta taatgccaaa
    15001 gtcgtgataa tttaaagaga agaggtgcac gaatgaataa ttttgatata ttccgcttca
    15061 aaccgtttat caatgaagcg ttaaccgcaa agggcttcaa acagcccaca gaggtccagg
    15121 aacgtttgat tcctttaatt gcaaaaggaa aaaatatcat tgggcaatct caaactggta
    15181 caggaaaaac acatacgttt ttgttgcctt tgtttgaaaa aatcaatgca gaaaaagagg
    15241 aagtacaagt tgttattaca gctccaagta gggaactagc tgcacagatc tatgaagcag
    15301 ctacacaaat cgctaaattt tctacaccgg agatccgtgt ctcgaatttt gtcggcggta
    15361 cagacaaaca gcgtcaatta aatcgtctgc atcaccaaca gccacaggtt gtagtaggca
    15421 cgcctggtag aatattagac atgatgaatg aacaagcgct gaagattcac accgcatttg
    15481 catttgttgt cgatgaagcg gatatgacat tggatatggg ttttttaaat gaagtagacc
    15541 aaattgcaag tcgattacca gaaaagctgc agtttttagt tttttctgcc actattccag
    15601 aaaaacttcg tccattctta cgaaaataca tggaaaatcc aatcattgaa gaaatcaaac
    15661 caaaagcagt tatttctgaa gacattgaaa attggctgat ttcaacaaaa ggtaaagatg
    15721 agaatcaaat gatttatcaa ctgctgacaa ttggtcatcc ttatttagca atcgtatttg
    15781 ccaatacaaa acagcatgtg gatgaaattg ccgattactt gaaaaaccaa ggcttgaaag
    15841 tagcaaagat tcatggagac atcaccccaa gagagagaaa acgagtgatg cgacaagtac
    15901 agaatctgga gtatcaatat gttgtagcga ctgatttagc tgctagaggg atcgatatcg
    15961 aaggtgtctc tcatgttatt aatgcagaaa ttccagaaga tctagacttc tttatccatc
    16021 gtgtcggacg tactggacgt aatggcttaa aaggaacggc cattacgtta tattcgccaa
    16081 atgatgagca agccattgat gcgatagaaa aattgggcgt ctcatttgaa ccaaaagaaa
    16141 tcaaaaacgg ggaaatcgtc aaaacatacg atcgtaatag acgtgccaaa cgagaaaaat
    16201 caaaagatgt attagatccc acgctgattg gtcttgtgaa gaagaaaaag aaaaagatca
    16261 agcctggtta taagaaaaaa atcgattggg caattgcaga aaaaaataaa aaagaacgca
    16321 aaatcgaacg cagacagcaa agccgtactg ctcgtaaaaa taagaaaaat tcacataaat
    16381 aaaaaacaag aggttgtgac aaaaggcgtg tcacaatctc ttgtttttaa gctagacttt
    16441 ttttcattcc gatacaaagt acttcatctt caataaaagg ttctgtgcaa gtatgatacc
    16501 cttgtttttc ataaaaatct acagccgtca tttcagctac tacataacta gtctgacaac
    16561 cttctgaaac cgcccgtttt tcagcagctt caagcaactg accgcctaac ccttttcctc
    16621 ggtattcttt tgctacgcaa aatcgttccg gcttgaagca agtaggtgtt ttcttttcat
    16681 aacgcaaagt agctactggt cttttttgat caagaaatag ataataggtt tgttgcggga
    16741 tatcttttcc gtcgaattct agttctgcag gaatcccttg ttctaataca aaaacttgat
    16801 atctcaaaaa gcagctagct gcgcgtatcc attcatccgc tccaactatt tttttcatca
    16861 tattcctcct tgtcatgaat agcagagatg tattgattcc tttattctta tcgtaccaaa
    16921 aatcgtggag tatgaccaaa aggaaatgaa aataaaaatt gagatattga cagtgatcaa
    16981 tagaatgcgt atactaaata agtacgaaaa ggatatgatt tgttttttaa gactcacaga
    17041 aaattctttc ttggctgaaa aaagaatagt cactaatcaa attgctccct ttttctattt
    17101 tggtagaaat accgacgcag ctcagcgtta tcgagtttaa gaggtaatga ttacacatca
    17161 ttgcaatcaa ggtggtaccg cgagttttcg tccttggctg tgatggtgtt tttttgtatt
    17221 taatttatag aaagcaggta atgacatgaa acaattaagt agtgcacaag ttcgacaaat
    17281 gtttcttgat ttcttccaat ctaaagaaca tacgattgaa cctagtgcgt ctttgatccc
    17341 cgtgaacgat ccaaccttat tgtggatcaa ctctggtgtt gcaacattga aaaaatattt
    17401 tgatggttca gttgtgccag aaaatccaag aatcacaaat gcccaaaaat ctattcgaac
    17461 aaatgatatc gaaaatgtcg gtaaaacagc acggcaccat acgatgtttg agatgctggg
    17521 taatttttcg attggtgact atttcaaaaa tgaagccatc cactgggcat gggaattttt
    17581 gactgatcca aaatggatgg cattcgatcc agaaaaactt tatgtaacag tttatccaaa
    17641 agatacagaa gcaaaacgca tctggcatga agaagttggc ctgccactag atcacattat
    17701 tgatgtagaa gacaacttct gggatatcgg tgcaggccca tgtggtccgg atacggaaat
    17761 tttctatgac cgtggagaag cttataacga tttagcagaa gacgatcctg aaaactatcc
    17821 aggaggagaa aatgaacgtt atttagaaat ctggaactta gtattttcag aattcaacca
    17881 taagcctgat aacacatttg aaccacttcc tcataaaaac attgatacgg ggatgggatt
    17941 ggaacgcatg gtctcaatcg ttcaagatgc gtcaacgaac tttgagacag acttgtttat
    18001 gcctattatc cacgccgtag aaaagctaag caaacaagta aaatatggtg aaaacccagt
    18061 aacagatatt tcttacaaag taatcgcaga tcatattcgc gccctttcat ttgctattgg
    18121 cgacggtgca ttgccatcga atgaagggag agggtatgtc ttacgccgtt tgcttcgtcg
    18181 tgctgtcatg catgggaaaa aattaggtat tgaacaagca ttcttatatc gtttagtccc
    18241 tgttgttggg gaaatcatgg taagttatta tcctgaagta cttcaacaaa aagaatttat
    18301 tgaaaaagtt atccgtacag aagaagaacg tttcaatgaa acaatcaatg aaggtttgga
    18361 aatcttaaat caagtgatcc aagaagtaaa aaacgaaaac gggacgactc tagcaggaaa
    18421 agacatcttc aaactatacg acacatatgg cttcccggta gaattaacgg aagaggtggc
    18481 agaagatgaa ggactgaaag ttgatcatgc tggttttgaa aaagaaatgg atgcacaacg
    18541 cgaacgtgct cgctctgccc gcagtactga aaaatcaatg ggggtacaat ctgctttatt
    18601 gacagatatc aaagtagaaa gtaaattcat tggatacaca gaacaagaat cagatagtca
    18661 attattggtc atcgttaaag gtgagtctct tttgaatgaa gtcgcagaag gtgaagctca
    18721 aatgatcttt gatcaaacac ctttctatgc agaaatgggt gggcaagtag cggatcaagg
    18781 atggatcatg aatgaagtag gcgaaatcgt agcagttgtt acagatgttc aaaaagcgcc
    18841 taatggtcaa tttatgcaca ccgtagaagt aaaagcacca atgagtgaag gacaaaccta
    18901 tcatctattg atcgatcaat cgatgcgcaa tcggatcatc aaaaaccata cagcaaccca
    18961 tctactgcac aaggcattga aagaagtact tggcgagcat gcaaatcaag ctggttcttt
    19021 agtcgctcct ggacatttac gattcgactt tacccacttt ggtcaaatca cttcagatga
    19081 attagctgaa atggaaagaa tcgtcaacga aaaaatctgg gaagctatcc cagttgaaac
    19141 aattgaaaca gatatcgata cagccaaaaa catgggtgca atggctttgt ttggtgaaaa
    19201 atacggacat gacgttcgag tagtaaatat tggcggctgg tcaatcgaac tgtgcggagg
    19261 cacacatgta gcaaatacag aaaacatcgg aatcttcaaa atcgtttctg aatcaggaat
    19321 tggtgcaggc gtgcgacgaa tcgaggctgt aacaagtaaa gaagcgtacg aactacttga
    19381 agaagaagaa aaacaactaa aacaagtagc aacaattgtt aaatcacctc aattgaaaga
    19441 agttgtttca aaagcggaac aattgcaaca gcaagtaaga gagctacaaa aggaaaatga
    19501 acaattagct agcaagttag cgaatcaaca agctggtgat gtattcaaag atccagaaac
    19561 gatcaatgga aaaacaatca ttgcagcaca agtaaaagtg aaagatatga atcagctgcg
    19621 tcaattagct gaccaatgga aacaaaaaaa tatttcagat attcttgttt tagcaaacga
    19681 acaagaagga aaagtcagtc tgctagcggc aatgacgaaa gaatcaaatg aagctggact
    19741 aaaagctggc gacttgatta aagcaatcgc accgaaagta ggcggtggcg gtggtggacg
    19801 tccagacatg gcacaagctg gcgggaaaaa tcctgctggt attccagatg cacttaaagc
    19861 tgcgaaagaa tgggttgcgc aacaataggg cgtttacgtt tgacagaaaa acaaaagttc
    19921 aaacattgaa cgaaacatga ttatgggggc gaagacaaac aagtcttgcc ctcatacttt
    19981 tttcaattgg atggcgcatc taatcaaatg gtcggattcg gatggtcttt agttgacgaa
    20041 agtgacagaa atttttacga ttagtataaa taaaaagggg tgaaaaaatg aatagtagaa
    20101 ataagacctt tcgactagtc ttacgtgcga tcttgttagc aatcatcatc gtacaagcga
    20161 tggttccttg gctggggttt attccattag gattcattag tttaacaatt atccatatca
    20221 cagtaattat tgctgcagta gtattaggcc caaaagatgg catggtgatt ggcttgtttt
    20281 ggggaatcgc tacaattgtt cgtgcctatg ccatgccaac aacgccattt gatacattag
    20341 tgttcacgaa tccgattatc tcggtagttc ccagagtact tgttggctta gtagcaggct
    20401 tggttttcca ctggatttat caaaaaaata aatcaataac gatcagctca gcctttgcag
    20461 gggtactggg ttcattggtc aatacagtct tagtgttagg attcatggga ttattttata
    20521 caggtgcaac ggcagatgcc tatggtgtag actctgctct tttatttaaa accttagcag
    20581 gggttgcagc aatcaatggg ataccagaag caataggtgc gggaatcata acaccattac
    20641 tagcaaaagc gttatttgca gcaacaccgt tgaaaccaga ataatgacca tcttttaata
    20701 gtaataggac gcacgataga tagaaaaagg aagagaggag aggaaaacaa tcgtcctctc
    20761 catcttttta cctgagcaac aagcaaacta gaaggggtaa aaaacttggc taagaaacaa
    20821 gaagaaaaaa agaaacaaat accgcaacag cggacaaaaa aaggagtact agcgggtatc
    20881 ttaggattgg ttacgaatct gatcttgttt gtctctaagc taatgatcgg gcttttttca
    20941 ggaagtgtct ccattatggc tgatgcagtg aacagtctat cagatactgc ttcatccata
    21001 ttgactttga tcggatttaa aattgctgca aagccagcag atcaggaaca tccttatgga
    21061 cacgaacgct tcgaatatat cagtgggtta tttgtttcga tcatcattac ttatgtagga
    21121 tttcaatttt tagagtcttc tgctgaaaaa atcatccatc cagaaaatgt ctctttaaca
    21181 cccatcgtct tttttgtcct aatcatttcc atctttttga aatttgcaca aggcaaaatg
    21241 tatcaggtta ttgcgcaatc cattgattct gaaacattga gagctacagc aacagatagt
    21301 tataatgatg tctttactac actggctgta ttgctttcag cagcaatcga atgggtaact
    21361 ggatggcgta ttgatggata tataggtttt gctttagcat tatatatcct ttatagtgga
    21421 ttgacgatgc ttcgaaattt tgtatacgaa ctattaggaa gtcgcccaac gccagcagag
    21481 attgaagaaa tggaagagca tttaaatgct tgcgatgata tacttggcta tcatgattta
    21541 ttggtgcata actatggacc caataaacgc tttgcatcag tccacatcga agtagatgaa
    21601 tcaatgaatt tgaatgtggc tcataagatt attgatctga tagaaaaaga ctttaaaaag
    21661 tctttggatg tggaacttgt ctgtcacttg gatccaatgc ctgttgataa cgaaaaatac
    21721 tataatatct tgcggcagtt aaagcaaatt cttcttgaaa caggtacagg gcttaatatt
    21781 catgatttta gagtaattca tcaaggaaaa acgctgcaat ttgatgtagt ggtccctgag
    21841 aaattccagt ttgttgatac agaactagaa agaatcttac gtagtcaaat agaagaaaaa
    21901 atcggagatt accaattgga aattacgttt gatcacaatt atcttttata ttaaatgaga
    21961 aactataaaa agactcctta aagtttttaa tttaaagagt ctttttttct tttaattgtt
    22021 tatggagagc ctgacgtgca agattatctg ctcctttatt tttgttttcg ggcaaccatt
    22081 gcaggatcaa taaagaaaat tcggatagaa gttcttggat tctttgaagg tagggaagga
    22141 agagctcgtt cttcgttgcg tttttgtcga ccgtagaaac tagtgttttg ctgtctgaaa
    22201 aacagaaaat cgtttcattc tgtaagtcgt ttttctttag aaattctaaa gtttttaaaa
    22261 agacttcgaa ctctgcttga tggtttgtcc ctatagaaag cgggatagat agctgttctt
    22321 gtttaccttg atagacaatc aagattccac ctccactagg accagggttg ccttttgtag
    22381 aggcatcaat gtatactttt aacataaaaa gagattctcc tttttgttca aactaatgtt
    22441 atggtaagat tatatcatta ctgaatcggt gagaggttag agaatgaaaa aaaatattcg
    22501 atggcagcct gaactggcac atacgatcat ctattggtca ttgaccttga tcattctgtt
    22561 ttttagtctg attctttctt tagaaaacac aggtccttat tggaaaagca attttgtttt
    22621 aggtattttt cttgtttttc tatttctagg tttgcggcgt tcttttattt taaaatcaaa
    22681 ctctgtgatt atacgttatg ctgctttttg gaaagaccga gaaatatctg tcggacagat
    22741 tcgatctttg acgccaacta atactggggt gcaaatgatg ttgcaagatg agaaagcccc
    22801 atctgttttc atgatgcgaa aaaaagatca agtagtcctt tttgatcatt ttgaaaaaaa
    22861 cggacccaaa tcagaatcaa ttccttatcc ttatacgaaa aaaagaaaaa gctaatttta
    22921 aaaatcagaa cctaaaaaaa cgaattttta caggaaaaga aaagagtgag acgtacggtt
    22981 catcccgttt agcgtctcac tctatttgtt tatctattca tgcatactag tatcggtagt
    23041 ctgaacgttg cctttcttga catgggcagc ttgtgttcct cgattccctt cgatgatctc
    23101 aaattcgacc acttgatttt cttccaaagt tttgaatccc tcttcttcaa tcgcagtaaa
    23161 atgtacaaaa atttcttctg tgtcatcata actgataaag ccgtatcctt ttttgttgtc
    23221 aaaccatttt acaattcctg tggtcaaagc actcacgacc tttctaaagt ctgatcatag
    23281 ccttcattat aaagagtatt tataagtacg tcaaataaat taaaaacctg taattttatg
    23341 ttttttttct gaataaaaaa aggtataatt ggcatatctt aacaaagaag atattttgtt
    23401 gaataaagtt cgtgaaaagg ggtaatcaaa tgaagaatga tctgcaaatt tcacaagaaa
    23461 cagagctaga accgattgtt gaagtggcta gtaaattaga tcttaaggaa gacgatttgg
    23521 aactgtatgg gaaatataag gcgaaaatca atttttctgg aatcaatcga tcaaaggata
    23581 acgctgaggg gcatttgatc ttagttactt ccatcaatcc aacaccagct ggcgaaggaa
    23641 agtcaacgat cactgtaggg ttaggagacg cgctcaataa aatcaataaa aaatctgtta
    23701 ttgcattgcg agaaccctca cttggtcctg tgatgggaat caaaggtggt gctgcaggcg
    23761 gaggatatgc acaggtattg cctatggaag atatcaactt gcattttact ggtgacatgc
    23821 atgctatcac aacagctaat aatgcattat ctgctctttt agataatcat attcatcaag
    23881 gaaatgaatt gaacatcgat gcccgccgag tgatttggaa acgggtagtc gatctcaatg
    23941 atcgtgaact gcgtaaagta attgttggct tgggtgggcc aatccaaggt gttccaagag
    24001 aagatgggtt tgatatcaca gttgccagcg aaatcatggc tatcttgtgt cttgcggcag
    24061 accttgaaga tttaaaggct cgtcttgcaa ggatcgttgt cggctataca tttgaccgtc
    24121 agcctgtgac tgctggagac ttgaaagcag aaggcgctct ggcattactg ctgaaagatg
    24181 caatcaagcc gaatcttgta caaacaattt acggaacacc agcttttgtc cacgggggac
    24241 cgtttgccaa tatcgctcat ggctgcaata gtattttagc taccaagaca gcattgagat
    24301 tagcagatta cgttgtcaca gaggcaggat ttggtgcaga tcttggcgga gagaaatttt
    24361 tagatatcaa agtacctaac ttgaaaaaag cgccggatgc tgtggtgatt gtcgccactg
    24421 tccgtgcatt gaaaatgcat ggcggaatga aaaaagatga acttaaaaat gagaatcttg
    24481 atgctttgaa agtaggtttt gcaaatttaa agcgccatat acgtaatatg gaacaatatc
    24541 agctacctgt gatcgtcgca atcaatgaat tcgtaacaga tacagacaat gaactcacac
    24601 tgttggagca cttgtgtgaa aatcagggga ttcttgccaa gagagcgagt gtatgggcag
    24661 atggagcaga aggcggcgtt gatttagcaa aagctgtagt acgattgatt gatagaaaag
    24721 aagctaatta caagccatta taccgcctag aagaaacgat acaagaaaag acagaaacaa
    24781 tcgtcaagaa aatttacgga ggaaatggtg ttgttttcag caaaaaagcg caaaaacaaa
    24841 tagaagagtt tacgaagaat ggatgggaca aactgccgat ttgtatggca aaaacacagt
    24901 attcattttc tgatgatcct tcactgctag gtgcaccgga agatttcacc atcacgatcc
    24961 gcgaaatcat cccaaaatta ggggcaggat ttttagtagc cttgactgga gatgttatga
    25021 cgatgcctgg tttgcctaaa aaacctgctg cattgaatat ggacgtgaca aatgacggag
    25081 aagtgttggg attgttttaa gcttgattat aatctaccat cgaaggagct gtattttgac
    25141 tacagcttct ttttttagac gatagggaat taattttgtt tacactagga tcattctttt
    25201 tctttattgt attttgagct atactaaagc ataataggag ggtatgatga aaacgacatt
    25261 tataagaaaa accggttttt acggtatggg tagctcgatt cagctaagaa tcaacaatga
    25321 aaaacgcagt gttctcagtc atgaacaaaa gataacgctt gatctggaag tgccctttac
    25381 gatgcaggtc accttttatt ggctaaaaag ccccgtttat acagtgaaag aagcaaagcc
    25441 tgcttatgtg atcacaatga attacttgat tcttcaaatc tatccgtttt tatttctagc
    25501 aactgggttg ggggccgttt tcctacaaaa tttatttttc agtttttttg cagttattgt
    25561 gttgattgcg ttctttttct atataaaaaa ccgggcatat ttgatcaagg aggaaaatta
    25621 tggcaaactc tgatcttttt ttaatcagtt tcaatcgaat cgaaaaatgg atgagagacg
    25681 agatgggaaa tgcacgaaat atgggattta ccgaacttgt cagacgccta gctcaaagaa
    25741 aacaattaat gatcagaaaa tacgaagatg atcttttaca gcttgctcag ttaaggaatg
    25801 cgatcgtcca tgatcggatc gcagtcgatt ttgtcattgc agagccaaat gattggacag
    25861 tcaagcgaat ccagctgatc gaacaagaac tgattcagcc tgaaaccgtg ctgcctcgat
    25921 ttgcaaaacg tgtaacaggt tttgaacaag atcttccgtt acttgaactg ttaaagatag
    25981 tagcagaaaa aaggtattca caatttccgt tatacaataa aggaaaattt gtagcattga
    26041 ttactttacg taatatcggg ttttggctag caaaagaaag ccaaaaaggt ccggttgatt
    26101 taacaaataa acgagcgttg gatttgatca tccaaaacgg aaagtacaca aattatcatt
    26161 ttgttccagc taaaacacat atttacgagg ttgaagcgat gtttcgtgag caagggactt
    26221 tggaagccgt acttatcact aaagatggga atcccgatgg taatctgctc gggatcgtac
    26281 gacctcgtga tatttataaa caaatagaaa aggattgatt ttttgttaat tatttattgg
    26341 atattcagcg ctttgctagt aggagtggat cagttggtca aatggtggat cactgataat
    26401 ctagcgcttg gagagacgaa aaatcttata ccaggtattt tgtcactgaa tcatattcga
    26461 aacactggag ccgcatggag tatgctagaa gggaagatgt ggttctttac tatagtgaca
    26521 ctcattgcag tcgttgtgat tctcacattg atgattaaaa atcgtgaaaa aggtaatcgc
    26581 tggttcatga ttggattgag tttgattcta gctggtgcga ttggcaattt tatcgaccgt
    26641 attcgtcttg gctatgtagt agatatgttc cagacagatt ttatgagctt tccgattttt
    26701 aacgtagcag atgtgacact tgtatgtgga gtgatttgta tgttaattta tattatttta
    26761 gatgaaaagg atcaaagaaa aaaatgacag aacttaaaac gacgattcac catgaatctg
    26821 gtaggatcga taaagtattg actaatttat tcaccgatta cagtcgttcg caaattcagc
    26881 tgtggctgaa aaatggagcg gtttctgtca atggacagcc agtcaaagca aattacaaag
    26941 taaaacaagg agacgagatt actgtcttga tccccgaacc agaaacatta gatgtggtcc
    27001 cagaaaatat cccgttgacg atcgtttacg aagatgaaga tgtagcggtg ataaacaaac
    27061 ctcaaggaat ggtcgttcat ccttcagcag gtcatacgca tggcacaatg gtcaatgcgc
    27121 tgatgtacca tatcaaggat ctttctacga tcaacggcgt tattcgtcca ggtattgttc
    27181 accgaatcga caaagatact tccggactgc taatggttgc caaaaatgac caggcacacg
    27241 aatcattagc tcagcaatta aaagataaga cctctttgcg caaatatgtt gctttagtcc
    27301 atggagagat tcctcacgaa aaaggaacca tcaatgcgcc aatcggccgt tcgaaggaaa
    27361 accggaaaat gcaggcgata agagaagatg ggaagtcggc tgtcacacat tttactgtat
    27421 tggaacgttt cgaaggattt actctggttg aattaaaact agaaaccgga agaacacacc
    27481 agatccgtgt gcatatgaag tatatcggtt atccgttagc tggagatcca gtatacggac
    27541 cgaagaaaac tttgaaaggc aatggtcagt ttctccatgc gcaaatgtta ggttttacgc
    27601 acccgactac aaacgaaaag atgatctttg aagcacctct tccagaagtt tttgaaaaaa
    27661 ctttagaaaa attaagaaaa aacattgcat tttaatttag ataacggtat agtaattaac
    27721 aacgaaagca taaaacttaa tagaattcct ttaagtttag tcccgtgagg ctaagaaggg
    27781 tgggagcaag gatacgtatg gtgcgccata agtagattgc tcattgacat agtatagcta
    27841 tcagtgtatc agtatacgct cagtctaaac attcctcctg tcctcttagg gcaggagttt
    27901 ttttatgcaa aaaagtaggg ggaatgaaaa tgcaagcaaa agaagtagta gatcaggtaa
    27961 ccatgaagcg agcattgact cgaattactt atgagattat cgagagaaac catagtatcc
    28021 aagatatcgt attggtaggt atcaaaacac gaggaatcta cattgcatca agaattgccg
    28081 aacgcctgaa gcagctagaa gatatcgata ttcctgtagg agaactggat atcactttat
    28141 accgagatga caaaaaagaa aatccagaag aaccggaact tcattcttct gacatcccag
    28201 tttccttaga aggaaaagaa gttattttga ttgatgatgt tttgtataca ggacgaacga
    28261 ttcgagcagc aatggacgca gtaatggatt ttggcagacc aagaaaaatt tcattagcag
    28321 tcctggtcga tcgtgggcat cgagaattgc caatccgtgc agattatgtt gggaagaaca
    28381 ttccaacatc tcgggcggaa gagattttag tagagatgca agagcttgat ggacaagatc
    28441 gcatcatgat tttgaaagag gaagattaga ggagagagaa acatgtcgga gaaaaaagga
    28501 tttcataatg atgacgtggt gttagatatc cacgatcgcc caaccccagg acattgggta
    28561 ggattaagtt tacaacactt gttcacgatg ttcggagcaa ccgtgttagt ccccatctta
    28621 gtaggtatcg atccaggtat tgcgctagtc agttcaggac taggtacatt ggtgtactta
    28681 tttactacta aaggaaagat acctgcgtac ttaggaagca gcttcgcttt cattgcagcg
    28741 atgcagcttt tgatgaaaac agatggttat ccagcgattg cacaaggagc agtcacaact
    28801 ggattagtct atttgattgt tgcgctgatc atcaaaaaga tcggttcagc atggctggat
    28861 aaaatcttgc ctccaatcgt cgtcggtcca gtcatcatgg ttatcgggtt gggacttgcg
    28921 gcaaatgcag caacaaatgc tatgtataat cagggcacgt atgatttcaa gtatattgct
    28981 gtagcgctga tcacattagg attaacaatt ttcttcaaca tgtggctaag aggttttctc
    29041 ggattgattc cgattttact aggtattatt agcggataca tcgtagcctt gctgtttgga
    29101 atcgtggata ctaagccaat cgtagacgct gcctggtttg ctgttcctaa ctttgagatt
    29161 ccctttgtac aatatgaacc aaagctatat cttggagcaa tcacaacaat ggcacccatc
    29221 gcttttgtga cgatgacaga gcatatcggc catcttatgg tattgaataa actaactaaa
    29281 cgtaatttct ttgaagaacc gggtcttcat aagacgttga caggtgatgg attagcacaa
    29341 atcgtggcag cctttgtcgg aggtcctcct gtaacaagtt acggtgaaaa cattggtgtt
    29401 ctggctatca cacgtgtcca tagcgtgttt gtgatcggtg gagctgcagt atttgcagtc
    29461 attttaggat tcgtaggaaa attaagcgct ttgattttaa gtatccctgg accagtcatc
    29521 tcaggaatca gttttatctt atttggagta attgccgcaa gtggaatgaa gattttaatc
    29581 gagaacaaaa tcgattttga taaaaagaaa aatctgttga ttgcttcagt tatcttagta
    29641 gtaggaattg gcggactcgt ctttgaagta ggcacattta cattgtcggc aatggcatta
    29701 gcaactgtac ttggcatcat tttaaatctt atcttaccag aaacttcacg aagcgaagaa
    29761 aactaaggag gaaacattat gatcatcaaa tcagaacgta tcagcttaaa gcatttattg
    29821 accgttgagg cacttactga tcaagaagtt atgggcttga tccgcagagg gcaggaattc
    29881 aagcaaggtg ctaattggac tccgcaaaag cgccagtact ttgctacaaa cctctttttc
    29941 gaaaatagta ctcgcacaca taaaagcttc gatgtagcgg agaaaaaact agggctagaa
    30001 gtgattgaat ttgaagcaag cacaagctct gtccaaaaag gggaaacact atacgataca
    30061 gtactgacga tgtcagcatt aggcgtagac gtagcggtta tccgtcatgg tgatgagaac
    30121 tattatgacg aactgataca aagtaaaaca atccagtgtt cgattatcaa tggaggagac
    30181 ggaagcggac agcatccaac ccaatgcttg cttgatttga tgaccattta tgaagaattt
    30241 ggtcattttg aaggtctgaa agttgccatc gtaggtgata tcactcactc tcgcgtagct
    30301 aaatccaata tgcagatgtt aaaacgtcta ggcgcacaag tattcttctc aggtcctaga
    30361 gaatggtacg acgatgaatt cgaagtgtac ggacattata tgccattgga tgaactacta
    30421 gatcaagttg atgtcatgat gatgcttcgg gtgcaacacg aacgccatga tggaaaagaa
    30481 agtttctcaa aagaaggcta ccatcaggaa catgggttaa cagttgagcg tgcgaagaag
    30541 ttgcaaaaac atgcaatcat tatgcaccca gcaccagtta accgtgatgt agaattagct
    30601 gattcattag tggaaggatt acagtcgcgg atcgttcagc aaatgagcaa tggagtattc
    30661 gtcagaatgg caatcttaga agcagtgcta aatggaaaag cgtaattata ggaggatgaa
    30721 aaaatgaaaa cactcattaa aaacggtcag atcaatacac gaaaaaatat gacgacacca
    30781 gcagaaattt ggatagaaga tgggcgaatc aaagcaatcg gtacaggatt ctcagaagcc
    30841 gaatttgatg aagtgtttga tgcaaaggga caattgatca cacctggttt agtggacgtc
    30901 catgttcatt tgagagagcc aggatttaca tacaaggaga cgatcgaagc tgggactcgc
    30961 tcagctgctc gtggcggttt cactactgta tgcgcgatgc cgaatttaaa tcctgtacct
    31021 gatacggcag aaaagctgag acaagtatat gacatcatta gaaaagatgc agttgtgaag
    31081 gtattgcagt atgcgccaat cacagaaaat ctgcgaagtg agaaattagt tgatcaagaa
    31141 gcactgattg aagaaggtgc ttttgctttc acaaatgacg gtgtaggtgt ccaaacagct
    31201 gggacgatgt atctggctat gaaagaagct gcaaaaaaca ataaagccct agtagcccat
    31261 acagaagatg aatctttatt atttggcggc gttatgcacg ctggcaagaa ggcagaagaa
    31321 cttggtcttc ccgggatctt aagcgtaacc gagtcttctc aaattgctag agatctgttg
    31381 ctagctgaag caactggtgt tcattaccat gtttgccatg tttctactaa agaaagtgtc
    31441 cgtgtaatca gagacgcaaa aaaagcagga atccacgtaa cagcagaagt ttcaccccat
    31501 catcttattt tgatcgacga agatattccc gaagatttcg gcttttggaa aatgaacccg
    31561 ccgctaagag gacgggagga cagagaagct ttgatcgaag gcttgcttga cggtacgatc
    31621 gattgcatcg ctacagacca tgccccccat ggattagaag aaaaaagcca aagtttcatg
    31681 aaatcaccat ttggtatcgt aggaagcgaa acagcgtttc aactgatcta tactcatttt
    31741 gtagaaactg gtcgattcac gttggaacaa gtgatcaatt ggttggcagt taaaccggct
    31801 gagatttttg gtttgaatgc aggaacgcta acggttggtg cccccgctga tgtagcagtt
    31861 tttgatatta cacaaacttg cacgatcgat aaagaagact ttttatcaaa aggggagaat
    31921 acacctttta tcggctggaa agtaaaaggc gaaacacaaa tgacttttgt caacggaaaa
    31981 cttgtgtggc agaagggaga ataaaagtat ggaacgtttg cttatattag aagatggaac
    32041 agttttcgaa ggaaaggctt ttggtgcaga tgctagtgtc ttcggagaaa tcgtttttac
    32101 aacaagtatg actggttatc aagaaactat cactgatcaa agttttaacg gtcaaatcat
    32161 tacatttacc tatcctatgg tgggtaatta tggggtaaat agagatgatt atgaatcgat
    32221 ttctccaaca tgcaaaggag tagtagtaaa agaacatgca cgtgtagctt caaactggcg
    32281 aaaccaaatg acgctagatt catttttgaa acaaaaagga attcctggaa tatcaggtat
    32341 cgatacgcgc gcccttactc gcaaaatccg tgaagtcgga acgatgaaag ctagtatagt
    32401 tgatgctgga gattccttcg aacatgcatt tgatcagctg aaagcaaccg ttctagcaac
    32461 caatcaagtg cagcaagtct caactaacaa accttatcca agtccaggaa caggtcggaa
    32521 agtcattgtc attgacttcg gcttgaaaca tagtatttta cgagaattga gtaaaagaaa
    32581 ctgtaacctc acagtacttc cctataacac agatgccgag acgattttag cgctatcacc
    32641 agacggagtc atgctgacga atggacctgg tgatccaaaa gatgtgccag aagcaatcga
    32701 aatgatcaaa aaaatccaag gaaaagtacc gatttttggt atttgtctcg gtcaccagtt
    32761 atttgcttta gccaacggtg cagataccta taaaatgaaa ttcggacatc gaggattaaa
    32821 tcacccggta cgagaagtag cgaccggaag aattgatttt acttctcaaa atcatggcta
    32881 tgcagtcgat gaagccacga ttgatccaga aaaactcatc gtaactcatg tagaagtaaa
    32941 tgacggaaca gtagaaggag tcaaacacag agattatccc gctttcagtg tgcagtttca
    33001 tccagatgct gcccctggtc cgcatgatgc actacattta tttgatgaat ttatggaaat
    33061 gatggacgca ggaaaggagc agtaaacaga tgccgaaacg tacagatatc aagaaaatta
    33121 tggtgattgg atctggtccg atcatcattg gtcaagccgc agaatttgac tacgcaggaa
    33181 cacaagcctg ccttgcctta aaagaagaag gatatgaagt ggttttagta aattctaatc
    33241 ctgcgacgat catgacggat aaagaagtgg cagatcaagt atatatcgaa ccgatcacac
    33301 ttgaatttgt atcaaggatt cttcgaaaag agcgtccaga tgctctcctt ccaactttag
    33361 gcggacagac aggcctcaat atggcaatgg agctggcagc atcaggtatt ttagaagaat
    33421 tgaacataga acttctagga acaaagcttt ctgcgatcga tcaagcagaa gatcgtgatc
    33481 tattcaaaca gttgatgaaa gaattgaatc agccaatccc cgagtctgaa atcgtcaata
    33541 cagtagaaga agctgtagat tttgcaaata aaatcggcta tcctgtcatc gttcgtccag
    33601 cctttacttt aggcggaact ggtggaggta tgtgtgaaaa tgaagaagaa ttacgtatcg
    33661 tatcagaaaa tggattaagt ctttcccccg ctacccaatg tttgattgaa cgaagtattg
    33721 caggttttaa agaaattgaa tatgaagtga tgagagattc ggctgataat gcaatcgttg
    33781 tctgcaatat ggaaaatttt gacccggtgg gtatccatac aggtgattct atcgtatttg
    33841 cccctagtca aacattgtct gaccatgaat atcaaatgct tcgtgatgct tctctttcga
    33901 ttattcgagc acttaagatc gagggaggct gtaatgtcca actcgctctt gatccacata
    33961 gttttaatta ttatgtgatt gaagtcaatc cgcgtgtttc tcgttcatct gcgttagcaa
    34021 gtaaggcgac tggctatccg atcgctaagc tagctgctaa aatagcgctt ggattaacat
    34081 tagacgaaat gaaaaatcct gttacaggaa cgacttttgc agaatttgaa cctgcattag
    34141 attatgttgt agctaaaatt cctcgctggc cgtttgataa gtttgaaaac ggagaacggc
    34201 ttttaggcac gcagatgaaa gcaactggtg aagtgatggc tatcggtcgg aatatcgaag
    34261 agtctttatt gaaagctgtg cgttctttag aaatcggtac acaccatatc gaacttcctg
    34321 agttggcaga catcacggac gatgacctaa tccaaaaagt ggttcgagca caagatgatc
    34381 gcctgtttta tgtagctgaa gctattagaa gagggtacac catagaagaa ttagcggatt
    34441 tgacaaaaat cgatttattc tttttagata aactgttgca tatcgtagaa atcgaacagc
    34501 agttgaaaca gcagccgttg gatgacaaga tattgaaaac agcgaaacaa aacggtttta
    34561 cagacaaaaa agtggcggag ctgtgggaaa taaacgaaga agccgttcgc cagatgagaa
    34621 aagaaaatga tttgattcct gtatataaga tggttgacac ttgtgcagcg gaattcgaat
    34681 caaaaacacc ttatttttat agttcctatg aatatgaaaa tgaaagttca cgatcagaaa
    34741 agccctcaat tctagtactg ggatctggtc ctatccgtat cggacaaggg gttgaatttg
    34801 actatgcgac tgtgcattct gtcaaagcaa tccagcaggc aggatacgaa gcaatcatca
    34861 tgaacagcaa tcctgaaaca gtttcgactg acttttctat ttcggataaa ttatattttg
    34921 agccattgac cttcgaagat gtgatgaatg tgatcgagct agaacagccg ataggagtaa
    34981 tcgttcaatt tggtggtcag acagcaatca acttagccga acctttgact gctgctggtg
    35041 taaaaatctt agggacaacg atcgaagact tggatcgtgc ggaaaatcgt gatttattcg
    35101 agcaagcgct gaaagagttg gatattcctc aacctttagg ggatactgct acaagtaaag
    35161 aagaagccgt ggttatcgct tcgagaattg gttatccagt tcttgttcgt ccaagctatg
    35221 tattaggcgg acgtgcgatg gaaatcgttg aaaaccagcg agatttagaa gattacatgg
    35281 aacatgctgt gaaagcatct ccagaacatc cagtactagt ggaccgttac ttgcttggtg
    35341 gtgaatgcga agtggatgcg atttgtgacg gtgaaacagt actcatccca gggatcatgg
    35401 agcatattga gcgtgctggt gtccattcag gggattcaat ggctgtgtat ccacctcaaa
    35461 cattatctga atcgatcaag aaaacgatcg aagattacac gatccgctta gcattaggtt
    35521 tgaattgtgt cgggatgatg aatatccagt ttgtcatcca tgaaaatgag gtttatgtga
    35581 tcgaagtcaa tcctcgagca agtcggactg ttccattctt aagtaaggtg acaggaattc
    35641 caatggcaca aattgcgacg aaagctattt tgggagagaa actaaagaca cttggttatg
    35701 aaaacggact ttatccagaa acaaaagaag ttcatgttaa agcacctgtc ttttctttta
    35761 cgaaattgca gaaagttgat acttacttag ggcctgaaat gaaatcgact ggagaagtaa
    35821 tgggatctga cagacaatta gacaaagctt tgtacaaggc attcgaagct tctggattac
    35881 acttgcctga atatggagcc gtattattta cgattgcgga cgaaacaaaa gaagaagcac
    35941 tagaattagc taaacgtttt acagaaattg gctatagtct gataggaacg aaacatacag
    36001 ctgcttattt cgaaaaaaat ggtctggtcg ttgaaacagt agcgaaaatt tctgaggaag
    36061 cagcagaaaa aaatgtagtc gacttaataa gagatggtaa aacacaagtg gttgtcaata
    36121 cgattgataa agatcgggga aatgcttcta aagacggttt catcatccga agagaagcag
    36181 ttgaacatgg tattccattg tttacttctt tagacacagc agatgcgatc gttcgagtga
    36241 tggaatcccg tgctttttca actaacccta tttaacggcg agcacatgtt aagacaaaaa
    36301 gacgaaaaac cgttgcagga ggggacagat gaaacaagaa atgatggaaa tcgttcagca
    36361 aaaacagctg gcaccaaaga tttttgaaat gacattgaaa ggtgatctag taaaagaaat
    36421 gcagcaacca ggacagttct tgcatattct cgtgccgcgt gcagacttgc tattacgccg
    36481 accgattagc ttgaatcata tcgaccacga aacaaatact tgccgtatca tttatcgggt
    36541 ggaaggtgag gggactagag tcttctctac tctttctgcc ggagaccatt tagatgtcat
    36601 gggtccttta ggcaatggtt ttgatttgtc caatcttcat acaggagacg aagtatttat
    36661 aatcggtgga ggaatcggtg tccctcctct atatgagtta tcaaaacagt tagtcgaaaa
    36721 aggaattaag ccaacgcatt ttttaggttt tgccacacat gaagtcgttt attatgaaaa
    36781 agaatttaat cgtctaggag aaacaagaat cgctactgac gatggaacat acggtatgca
    36841 cggaaatgtc ggtaatttat tgttaggaac gaagacggaa cctacagctg tttttgcatg
    36901 tggttcaaac gggttattga agacagtgga acagctgttt tcttcgcatc aaaatgtcca
    36961 gttatcatta gaatcccgca tggcatgtgg gataggggct tgctatgctt gcgtttgtca
    37021 tacgccggaa gatgaaacag gaacaaaaag tgtgaaagtt tgtgacgaag gaccgatttt
    37081 taaaatagga gaggtaatta tatgaatgag aatcctttag cggtcaaact tcctggccta
    37141 gaattaaaaa atcctatcat gccggcaagc ggctgtttcg gttttggcaa agaatacgga
    37201 aaatactatg atttgaatca gcttggatcg atcatggtca aggcaacgac tccaaatgcc
    37261 cgctttggca atcccacgcc aagagtggcg gaaacaccat ctggcatgtt gaatgcaatt
    37321 ggtttgcaaa atcctggtct agacgttgtt atgcataaac ttcttccaga attagaggag
    37381 tacaatgagt tacccatcat cgcaaatgtt gctggtgctt gtgaagaaga ctatgtagaa
    37441 gtttgtagca aaattggcga agcaccaaat gtgaaagcaa tcgagctgaa catttcttgc
    37501 ccaaatgtca aacatggcgg aattgctttt ggaacagatt ctgatgttgc ttttcaatta
    37561 acacaagcag taaagaaagt ttcttctgtt cctgtctatg tcaaattgtc cccaaatgta
    37621 acagacatcg ttccaatcgc tcaggcaatc gaagctggag gggcagatgg gtttacgatg
    37681 atcaatactc ttcttggtat gcggattgac cttaagacaa gaaaaccgat tttagccaat
    37741 caaacaggtg gactctccgg accagcaatc aagcctgtcg ctattcgttt gattcaccaa
    37801 gtagcaagta tatccagcct tccaatcatt gggatgggcg gcgtgcagac agtggatgat
    37861 gttcttgaga tgtttatggc tggagcaagt gctgtagcta tagggaccgc gaatttcacc
    37921 gatccgtata tctgtcctaa actaattaaa gagctccctg tgcgaatgag cgaactaggc
    37981 atcgaatcgc tggaaagatt aatcaaagaa gtgagagagg aacgtgaacg atgaatcaac
    38041 tagaccaacg accaattatt gcattagatt tttcaactag gcaagaggta gaagactttc
    38101 ttcgattttt cccgaaagaa gaaaaattgt ttgtgaaaat cggaatggaa ttgttctacc
    38161 aagaaggacc tgaaatcgta cgttatctaa aagaggctgg tcataatgta tttttagatc
    38221 tgaaacttca cgatattccg aatacggtcg aaaaagcaat gcggggattg gcaaagcttg
    38281 gtgttgatct cacctgtgtt catgccgctg gcggtattcg tatgatggag gcggcaatgc
    38341 gaggattaga agaggggact ccagaaggtg gaaaacgtcc gctacttcta gctatcactc
    38401 agcttacttc tacaagtgaa gaagaaatgc atgccgatca actaatcgaa gtttctttag
    38461 aaaaaagtgt gatccattat gctagctgtg ccaaaaaagc aggattagat ggtgtggttt
    38521 cttctgcttg ggaagtagaa gcgatcaaag aaatggcagg agacgaattt gtttgcttaa
    38581 ctccgggtat aagaccagaa ggaacagtcg caggagatca gacaagagta gtcacacctt
    38641 ctcaggcaaa aaagatcggc tcaactttta tcgttatagg tcgaccaatc actcagtcaa
    38701 ctaatcctta taaagcatat caaacgattc aaacagaatg gagctaacct aaaatgaatg
    38761 ttgaacaaag tatcgcaaaa gacttattag aaattgaagc agtattttta aatccaagta
    38821 atccttttac atgggcaagt ggaatcaaaa gcccaattta ctgtgataac cgaatcacga
    38881 tgagctatcc aaaagtccga aaagagatcg caaaaggatt agcaagcaaa atcaaagaag
    38941 cttaccctga agtacaagtg attgctggaa ctgcaacagc aggaatccct catgccgcat
    39001 gggtagcgga aatactggat ttgccaatgg tctatattcg aagtaaggcg aaagatcatg
    39061 ggaaaggtaa ccaaatcgaa ggacgtatat ttgaaggtca gaagatggtt gtgatcgagg
    39121 atttgatttc tacaggcgga agtgtcttgg aagcagctga agcagcaaaa agagaaggtg
    39181 cggatatcct tggtgttgcg gctatcttta cgtatgagtt gccaaaagga aaagcaaatt
    39241 tcgaaaaagc cgaaatccct cttatgacgt tgaccaatta ttctgtattg attgaagcag
    39301 ctttagaaga tcgttatatc gatgagcaag aattaactct gctaaaagaa tggaaaaaag
    39361 atcctgaaaa ttggcagatt taagcgcgct tgatgaagat agagaaatcc gatttgtgtc
    39421 tccagaatca tcaattggaa tagcaaaaat ccaaaaactg attgcgagaa gcatcttttc
    39481 atcaggtatg aaaagaaact tctatcgtga tcagtttttg atttttttaa aattcatttt
    39541 tctaagactg gttagcagaa agaagagaat aatctcttct cttttttcta ttatatatag
    39601 attttctcta ttttttggta agggagctgt ttcaaaaatg gtaaagagcg gaaatgttgc
    39661 gaatatcagt tatttaatga tatgatattc ggaaaaagaa atctcaaagg gaggtaactt
    39721 ttatggggtt gaatgaattt attttaggat tgaacaattt agagacgatc acaagggcac
    39781 ctggattttt taaatataca gagcatacag ttgcagcaca ttcgtatcgt gtagcatcta
    39841 ttgcgcaagt tctaggagac attgaagaag cgaatggaca accgatcgat tggaaactgc
    39901 tctatgaaaa atcattgaat catgactaca cagagcgttt tattggtgat atcaaaacgc
    39961 cggtgaaata cgcaagcaaa gagttaagag ggatgctgca aacagttgaa gaaaagatga
    40021 cggacgaatt catcaaccaa gagattccag ctgagttcca agaagtttat cgccgtcggt
    40081 tgttcgaagg aaaagatgaa accgtagaag gagaactatt agccatttca gataaggttg
    40141 accttcttta tgaatctttt gatgaaatca tcaaaaataa cccggaaaaa gtttataagg
    40201 agatgttcat tgaggcagtg actacgatta aaagctttga acatcggcct tctgttcagt
    40261 atttctttaa agagatattt ccggaactat tgaaccagga attttatgga aaagaggcgt
    40321 ttttagaaac cgtctcaacg attgtaaaga aagaagaaaa ataaaaatga tatgagatgc
    40381 agggggaagt atagttgaaa aagaatatgg atgtcgaatg gggctattct ggagacaaag
    40441 gtccggaaaa ttgggcgagt ttatgtgatt ggtttgcgag aggtgcggaa tttccattgc
    40501 agtcgcctat tcatttgacc aagggggaag aaaccaaaat agaaggagac acgctttcct
    40561 ttcattatca aaagcaacgg tttactgaca aggaattcaa aaacacgatt catttagtac
    40621 cttttgacca actcagttat gtagagttca agaaagaacg ctattatttg acagatattc
    40681 atttccactt gccttctgaa catatcatca acggaaaaca ggaagatatt gaatttcatt
    40741 ttgttcatat gaacagcaaa aaagagaatc tagttgtggg tgtcatgtac cagctaatgg
    40801 tacaggaagg ttggctgtat aatcaagaaa atggggaaag ttggaactta gaaaaacatg
    40861 agcattgggt aaatccggat gtgttctttc cggagcaaaa aagccatttc cattatattg
    40921 gctcattgac aactccgcct accagcgggc caatccaatg gtttgtaatg gatcatgtag
    40981 gaaaactgaa ccaagagttt cttctcccct tcgatggtca gtatgctcga ccgaacaacc
    41041 gaccaatcca gcccttgaat ggaagagaaa tttattattt cgaggagttt gagcaatgaa
    41101 tattcagcag atgaaatacg tggtcgctgt agcaaacaat ggtagtttca gagaagcggc
    41161 aaagaaaatg tttgtctccc agccaagtct ttcagctggt atcaaagaat tagaaaccga
    41221 gttagggatc acgctgttta ctcgaacgaa tcgaggggca tatttaacag aagaagggca
    41281 agacttctta aatcgtgccg agaaaatatt ggtccagctt gatacattag aaactcacta
    41341 tctaacgagt gaaaagagag acaatttttc gatcgctgcc cagcattatg atttcttagg
    41401 tcctttgacc gcacagctac tcgaggaatt caaagacgag atcaaacaat ttcggatcat
    41461 ggagacgaca acagcgagag tgatcgaaga agtcaaagag cagcacagtg agatcggcat
    41521 tatttatatg aatgagcata atcaagctgg tatcagaaga tatttacaac aaggagagct
    41581 aagtggccgc agcattggaa aattcaaaac gcatattttt ttaaggcaag gacatccatt
    41641 agctgaacac gcagagatca ccaaagaaga attgttatct tatcctcaag ttcgttttac
    41701 acaggatgga aataatttcc cgtattttta tgaagacctt atcgagatcc ctgatcaaga
    41761 aacagtgatt tatacgagtg atcgaggtac tttgatgaat atcgttcttg aaactgatgc
    41821 ttacgcttca ggatctggta ttgtgattgg agggattcgc gagcacttgc ggttaatccc
    41881 tttggcagaa ggcgaactaa atgaatttta tatcatttat tcggcgaaac gtccgctcag
    41941 tgaaatcgct caaagattta tcaaagagct tactcggtta ctggaccagc agagctaaga
    42001 aaagcagatg agaacgctgt ttattgaaca ccgttgatca aaaaagaggg gttgtgacag
    42061 aattttgtca caactcctct ttttgtagca aacaatttat aactaattgc tgttgtggga
    42121 aagtacgcaa atatgattct agaaacactc attgattacc tgttttgttt catagctgta
    42181 attttttctt cttctggtgt cacgataatc gtcttttggt tttcataaat aacataacca
    42241 ggcttggcgc catttggttt tcggatatgc tttacctgta caagatcaac cggcacttgg
    42301 gcagagtatc ggtattttga aaaataggca gctaactcgg cagcttctgt aattgtttca
    42361 tctgagggct tgtcactttt aatgatgacg tgcgaaccgg gtatattttt tgcatgaagc
    42421 caatagtcgg tttttttagc tgtcttcatt gtcagctggt cattttgaag attgttttta
    42481 ccaacaagaa tgagcgttcc gtcagaagaa aagtattgat caggctgaga tggtttctta
    42541 cgtttttgtt tttttaagtt cttctttttc aggtatcctt ctgaagtcaa ttcttcttta
    42601 attgcttcga tatccattgg gccggcaatt tccagttgag ataagacaga ttcaagatat
    42661 tggatttcat ctttggcttc ttggatttgt tcgccaatca gcttaacagc gttcttcagc
    42721 ttttggtatc gatggaaata tttctgtgca ttttgatttg gtgtcaatgc cggatctagt
    42781 gctattttga ttggccgatc ttcttcataa taattaggta aaacaacttc ttttgcgccg
    42841 cgtggtactt gtgtcagaaa agtagtaagt agttcgccat ttctacggta attctcagcg
    42901 ttttcggatt ctttgagtgt ttgttcacgt tttttcaatt tatttttatt tcgttttagt
    42961 tcattttcga tttttcgaat cagttctccg ccttgttgtt ttgcccgatc tttctctgct
    43021 ttttcgtgat aatagctatc taataattga ctgagtgttg gataagctgt tacagcagat
    43081 gcctgttcgg acaatacctg ataagcaatc ggtgtgaaaa aatctttatt ttccgtttcg
    43141 taaaaagtag gaataggctg atggctgatg gctgagaaaa attcgtccca tacaaccatc
    43201 ttttcattag gacgttccag taatcttttg gtcagctctt gagcgatatc gaaaccgatt
    43261 ccctgaaact gacgttggat tgcttttgga tctagttcag tttgggaaag ccgctgaaag
    43321 attttttctt tctcggagct aaatgggttc aagacctctt gttttggcgg agcaacatat
    43381 tctacaccag gaagcaaaga acgataagtg ttttgagagc ttccaatgtg tttgatcgca
    43441 tccagtattt ttccagtttc tcgattaact aagacgatcg tactatgacg tcccattaat
    43501 tcaacgatca atatgatatt ttgtagatca cccaactcgt ttcgtttagc aaagtggaaa
    43561 tggatcactc gatcattttc gatttgttcg atgtcttcta aaatagcgcc atccaaatat
    43621 tttcgaagca tcatcacgaa gttaggcgga ttatcaggat tctgataatc gatttgggta
    43681 atctgtacac gagcataact tgggtgcgca gataataata atcgatggtt tttgccgcgt
    43741 gaacgaatca ccagcacgat ttcattttca taaggctgat ggattttcga gatccggcca
    43801 gatagtaacg tttcacgcaa ttcgttgacc atcgcgtggg taaatacacc atcaaaggac
    43861 atggttcatt cctcatttct tttcataagt atattcatta taacgaaaaa aagaagaaaa
    43921 accaatttgg ccgaatagta aagcaaaagt ctagaaaaat cttggactaa ttacataaaa
    43981 aacagtgttt ctatcgttga aatccaaatt tttaggtata ataaaaaata ggagaaaaag
    44041 tagaaaagaa ggtgtttgtt atttgggaag tagttaaatc gagagtacaa gaaatgaatg
    44101 aacatttctc agtgtcattc gcagaaaatc caagtaagta ttttcgacgt cttgtttttc
    44161 tgctcatctt atcattgctg ttaacagttg caatgttttt attgccggaa aaaatccgag
    44221 aagagcaagt aaatagtctc gtttattcgt ccataccaac cagcaaattg aaaccttttg
    44281 attacagcaa agctgatcag aaaatcaaac aaagccgggc gatatcggtg atgttcagca
    44341 aaccgaacgg gaaaacctat caggacatgc ttgatgtttt taatgatcct gagcttatgg
    44401 aagaattgaa tcgaccgatc ttttattatc cgatcgttta tgatgtacat gaactggaac
    44461 aaaaatataa catacgaacg gatgaagtga catttatttt ctttgatgga ggaaaagaag
    44521 caaatcggat aactgcgggt aagggaggaa taactgattt tgataaggag ttgatccctg
    44581 aactgaatcg acttccgtta gccaatatca aacaattaga agaagaattg gatagaacgg
    44641 gagtaacttc ccaaaattcc gagattgcta attgagacat tggcgcgatg aaacggtggg
    44701 tctatatagc gctattttta attatcagaa caagtgatgt ttgatcttca aaataacaaa
    44761 taaaaaatta aaaaatatag ttgacgaaat tcaaaaattc gattagactt aaatcaattc
    44821 ttaaggaaag ggtgacctgt aatgaactta tcaaaacgta tttcagcaga aaacaaaaaa
    44881 aattggcgca gctggcgtag ataagttgta ttaatggtga gactttccca tagatacaac
    44941 tttttagagt gcatttctaa agcttgtatc tatgctagac atgaacattg actgccgcat
    45001 agattagaat ctatgcggtt ttttgtgtaa agacaatgac cgctttggcc attgtctttt
    45061 tttgtagact ggatacttgc ctgattataa taaataaaaa gattgacttt gcaggaggaa
    45121 aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt atttagcagg
    45181 tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga accagacaaa
    45241 aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag atgagattta
    45301 tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa atcttgagat
    45361 caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc aacagctagt
    45421 acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat cattagcaaa
    45481 cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg gggctggatt
    45541 agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc agccgcctgt
    45601 tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag tcggaatctt
    45661 gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag aagctgcaaa
    45721 aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga ttgcacaaac
    45781 ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata ataccattgc
    45841 caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg tcattccatc
    45901 tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc aatatcaatt
    45961 agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa aaccggaaac
    46021 gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc aagctgatcg
    46081 cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta cagatgactc
    46141 gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc acaaggcatg
    46201 ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga ctttcctgat
    46261 atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc tattactcat
    46321 ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat agcaggactc
    46381 gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg gattttagtg
    46441 atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct ttctttattg
    46501 aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta ctttgacaca
    46561 gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt cttcttcaat
    46621 acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc tcggtcgtta
    46681 gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg tgtcattgca
    46741 ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa agggattggc
    46801 gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca agaattgacg
    46861 ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct attgatttta
    46921 gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc tatcatctta
    46981 gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac gggttttgag
    47041 aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa gtcgtaaaca
    47101 atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc agagaagggg
    47161 acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc aatacgatcg
    47221 ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg atcacaaaag
    47281 attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc aagatgggaa
    47341 cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga ggtcaaaaac
    47401 ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga ctatgtcaag
    47461 aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta tctggtggtc
    47521 aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta ctattattag
    47581 atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga ttaacaaatc
    47641 agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg gaagacgcct
    47701 tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa gatatttctg
    47761 aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa gaagtagtgg
    47821 attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat tgacatcttc
    47881 ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt gttttgtagg
    47941 atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga caagtaaacg
    48001 atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg tatcaaacgc
    48061 taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt ctggacaaac
    48121 caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat tgatgcacaa
    48181 tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag taaatccaat
    48241 gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg taaatctttc
    48301 gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg aagcgcaatt
    48361 gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct ataatactga
    48421 tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa agaaaaaaat
    48481 gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagcag cgttagacgg
    48541 cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag taaaagaaac
    48601 attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact ttgccgatac
    48661 gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatgcaa cagatatcgg
    48721 gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc atgagaatct
    48781 agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag aagcaaaaaa
    48841 agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag caatcgcgtt
    48901 tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt ttgattaaaa
    48961 aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa agtaatcctt
    49021 tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat gataggcttt
    49081 tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa ccacaatgag
    49141 tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc gggatacggg
    49201 gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg aagatgctac
    49261 ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg agcaacagat
    49321 atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt tcaatcggca
    49381 aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa tcattctatc
    49441 gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta aatatgtagc
    49501 tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc aaaaagcgac
    49561 gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag atgccgggcg
    49621 agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga aactgatttc
    49681 ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga tcaattcagc
    49741 ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt ttggaatcga
    49801 ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg atgccttatt
    49861 catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg tagatattaa
    49921 aaaagggggc cagaagatgg atggacatgt tcttttgcag tacgcacgtt tccgaatgga
    49981 tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg ctattttcag
    50041 ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta aagctttagg
    50101 atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt cgattgcacg
    50161 aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga attatgggca
    50221 aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat ctgctattac
    50281 agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa ggcttttgtc
    50341 atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat ttaaccaaga
    50401 gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg tgaagccatc
    50461 gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata cggtgaaatg
    50521 cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac gaatgcacgg
    50581 ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat tggtcctgaa
    50641 ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga agaaacgaag
    50701 cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga tgctgttccg
    50761 tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca gattaaccaa
    50821 gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg atcatcgttt
    50881 gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt attgccagat
    50941 gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga ggtcagaaga
    51001 tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc gaaaccttcc
    51061 aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt gctggtgaag
    51121 taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga gcttatgaag
    51181 agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc ttccgttacg
    51241 aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg cagtcagccg
    51301 ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg tggtcgatta
    51361 ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg atcaattctc
    51421 ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag aactattttc
    51481 caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga atgaatggaa
    51541 gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt tctcttggtc
    51601 gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc aaacacttca
    51661 aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg cagttttctg
    51721 gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag gaaatagaaa
    51781 aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg aaaaaggaaa
    51841 agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag atgaatcagc
    51901 taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac gaaagcaagt
    51961 ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat cagtatctgg
    52021 ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag acaactgaga
    52081 tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat gcaattattc
    52141 cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta cagcaagcaa
    52201 tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat atctacgaag
    52261 agacgaccgt aatgacgggc gtacagcaat taaagctaaa aagcatcgga aagatcccat
    52321 tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa gctcagactc
    52381 ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg gagaaagaaa
    52441 cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa actgcgggaa
    52501 aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga atagggacag
    52561 aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc gaagtcaata
    52621 aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta gccaatgaac
    52681 cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga aaaattggta
    52741 aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc ctacatttat
    52801 tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt agcgggacac
    52861 gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca aaatgtaaaa
    52921 agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa tgcgacttca
    52981 aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga gatttgcttt
    53041 caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg aaattcgatg
    53101 cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg atcgaaaaca
    53161 gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat gaaaacgaag
    53221 tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga gaatataaat
    53281 accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag gctgatttta
    53341 tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa attatcttat
    53401 gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg attacgggac
    53461 gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga ttttatcgac
    53521 tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa gcatgctaaa
    53581 gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca ttatttattt
    53641 tctaccagtt attacacaag tatttcatca atattttggt taagaatacg agagtttgtg
    53701 acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa agcaaaataa
    53761 aagtatatac attgattaaa agtatatact tggatataat gtacttacta tagaatgaga
    53821 atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta gaaaatctgg
    53881 caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg tgcagacagg
    53941 tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca aagcacaaga
    54001 agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag acgcgttaaa
    54061 agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat caacgcgaaa
    54121 gttattttac cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa atgcattaga
    54181 tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc ctgaaatcta
    54241 tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc ataatggcaa
    54301 taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt gtccagttgc
    54361 tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt cattggattt
    54421 cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa aatgaacgaa
    54481 ttaaaaactt tatttctcaa ttagatcagc atgtcatta
//
LOCUS       DAFGZE010000011        54468 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.011, whole genome shotgun sequence.
ACCESSION   DAFGZE010000011 DAFGZE010000000
VERSION     DAFGZE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.011"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            2..370
                     /locus_tag="LTU54_000661"
     CDS             2..370
                     /locus_tag="LTU54_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBL8295396.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="LTU54_000662"
     CDS             550..1491
                     /locus_tag="LTU54_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBL8295397.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="LTU54_000663"
     CDS             1560..2291
                     /locus_tag="LTU54_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBL8295398.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="LTU54_000664"
     CDS             2435..4615
                     /locus_tag="LTU54_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBL8295399.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="LTU54_000665"
     CDS             4635..5117
                     /locus_tag="LTU54_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HBL8295400.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="LTU54_000666"
     CDS             5133..5894
                     /locus_tag="LTU54_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL8295401.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="LTU54_000667"
     CDS             6025..7359
                     /locus_tag="LTU54_000667"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBL8295402.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="LTU54_000668"
     CDS             7666..8151
                     /locus_tag="LTU54_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HBL8295403.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="LTU54_000669"
     CDS             8144..8827
                     /locus_tag="LTU54_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBL8295404.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="LTU54_000670"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="LTU54_000670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBL8295405.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="LTU54_000671"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="LTU54_000671"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HBL8295406.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNTYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="LTU54_000672"
     CDS             12443..13072
                     /locus_tag="LTU54_000672"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBL8295407.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="LTU54_000673"
     CDS             13124..13456
                     /locus_tag="LTU54_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL8295408.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="LTU54_000674"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="LTU54_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBL8295409.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="LTU54_000675"
     CDS             14282..15655
                     /locus_tag="LTU54_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL8295410.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="LTU54_000676"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="LTU54_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HBL8295411.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="LTU54_000677"
     CDS             16710..17150
                     /locus_tag="LTU54_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL8295412.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17294..17995
                     /locus_tag="LTU54_000678"
     CDS             17294..17995
                     /locus_tag="LTU54_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HBL8295413.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17992..19113
                     /locus_tag="LTU54_000679"
     CDS             17992..19113
                     /locus_tag="LTU54_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBL8295414.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19128..20360
                     /gene="pepT"
                     /locus_tag="LTU54_000680"
     CDS             19128..20360
                     /gene="pepT"
                     /locus_tag="LTU54_000680"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBL8295415.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20492..21400
                     /locus_tag="LTU54_000681"
     CDS             20492..21400
                     /locus_tag="LTU54_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBL8295416.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21435..21707
                     /locus_tag="LTU54_000682"
     CDS             21435..21707
                     /locus_tag="LTU54_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBL8295417.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21718..22764
                     /locus_tag="LTU54_000683"
     CDS             21718..22764
                     /locus_tag="LTU54_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBL8295418.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23015..25624
                     /gene="clpB"
                     /locus_tag="LTU54_000684"
     CDS             23015..25624
                     /gene="clpB"
                     /locus_tag="LTU54_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBL8295419.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(25775..26461)
                     /locus_tag="LTU54_000685"
     CDS             complement(25775..26461)
                     /locus_tag="LTU54_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295420.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(26439..26933)
                     /locus_tag="LTU54_000686"
     CDS             complement(26439..26933)
                     /locus_tag="LTU54_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL8295421.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(26953..28173)
                     /locus_tag="LTU54_000687"
     CDS             complement(26953..28173)
                     /locus_tag="LTU54_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295422.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28320..30155
                     /gene="lepA"
                     /locus_tag="LTU54_000688"
     CDS             28320..30155
                     /gene="lepA"
                     /locus_tag="LTU54_000688"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBL8295423.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            30351..31262
                     /locus_tag="LTU54_000689"
     CDS             30351..31262
                     /locus_tag="LTU54_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295424.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            32258..32509
                     /locus_tag="LTU54_000690"
     CDS             32258..32509
                     /locus_tag="LTU54_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295425.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            32592..32864
                     /locus_tag="LTU54_000691"
     CDS             32592..32864
                     /locus_tag="LTU54_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295426.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            32954..33193
                     /locus_tag="LTU54_000692"
     CDS             32954..33193
                     /locus_tag="LTU54_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295427.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            33340..37623
                     /locus_tag="LTU54_000693"
     CDS             33340..37623
                     /locus_tag="LTU54_000693"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HBL8295428.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            37722..38087
                     /locus_tag="LTU54_000694"
     CDS             37722..38087
                     /locus_tag="LTU54_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295429.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            38089..38541
                     /locus_tag="LTU54_000695"
     CDS             38089..38541
                     /locus_tag="LTU54_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295430.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            39155..40465
                     /locus_tag="LTU54_000696"
     CDS             39155..40465
                     /locus_tag="LTU54_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HBL8295431.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            40488..40799
                     /locus_tag="LTU54_000697"
     CDS             40488..40799
                     /locus_tag="LTU54_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295432.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            41160..42416
                     /locus_tag="LTU54_000698"
     CDS             41160..42416
                     /locus_tag="LTU54_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL8295433.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            42413..42958
                     /locus_tag="LTU54_000699"
     CDS             42413..42958
                     /locus_tag="LTU54_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295434.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            42959..43459
                     /locus_tag="LTU54_000700"
     CDS             42959..43459
                     /locus_tag="LTU54_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295435.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            43540..44096
                     /locus_tag="LTU54_000701"
                     /pseudo
     CDS             43540..44096
                     /locus_tag="LTU54_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            44116..44460
                     /locus_tag="LTU54_000702"
     CDS             44116..44460
                     /locus_tag="LTU54_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295436.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            44479..45390
                     /locus_tag="LTU54_000703"
     CDS             44479..45390
                     /locus_tag="LTU54_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8295437.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            45469..46461
                     /locus_tag="LTU54_000704"
     CDS             45469..46461
                     /locus_tag="LTU54_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL8295438.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            46458..46676
                     /locus_tag="LTU54_000705"
     CDS             46458..46676
                     /locus_tag="LTU54_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295439.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            46689..47099
                     /locus_tag="LTU54_000706"
     CDS             46689..47099
                     /locus_tag="LTU54_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295440.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            47151..49679
                     /locus_tag="LTU54_000707"
     CDS             47151..49679
                     /locus_tag="LTU54_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8295441.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            49695..51905
                     /locus_tag="LTU54_000708"
     CDS             49695..51905
                     /locus_tag="LTU54_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295442.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            51913..53004
                     /locus_tag="LTU54_000709"
     CDS             51913..53004
                     /locus_tag="LTU54_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL8295443.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            53025..53567
                     /locus_tag="LTU54_000710"
     CDS             53025..53567
                     /locus_tag="LTU54_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295444.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            53762..54019
                     /locus_tag="LTU54_000711"
     CDS             53762..54019
                     /locus_tag="LTU54_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295445.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            54033..54365
                     /locus_tag="LTU54_000712"
     CDS             54033..54365
                     /locus_tag="LTU54_000712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295446.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
BASE COUNT        18506 a         8909 c        11728 g        15325 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tacttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat aattatctag
    17281 aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag tgggtgactt
    17341 tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact tacccgttgc
    17401 attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc aaggtcctta
    17461 tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca ccgtacgcct
    17521 tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca cgattgcggg
    17581 tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc aacgcttaag
    17641 cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac gatcttggct
    17701 gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata aaaaaatata
    17761 tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa tggagctgat
    17821 gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat ggacaagaga
    17881 gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc aagaggcacg
    17941 tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc catgacaatc
    18001 agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct agctgaagaa
    18061 ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg agtaatgatg
    18121 acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat cgatttgctg
    18181 attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga tcagccacaa
    18241 gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc acacacgaac
    18301 atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg tattgaagtg
    18361 gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt ttatgtacct
    18421 gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg tacacaaggt
    18481 gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc ggaagctggt
    18541 gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc aaaagttgag
    18601 gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc agctcatcct
    18661 tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat gtttgggtta
    18721 ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga acaagtaaaa
    18781 gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa atcctctgtg
    18841 aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc aatcgcccaa
    18901 agagcagatg tctatatcac gggagatatc tattatcata cggctcatga tatgcaaagc
    18961 gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa gcaacgattt
    19021 atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt tttcgtttct
    19081 gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg tacgaaaatc
    19141 ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca acaagcaaaa
    19201 cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa gaattagaag
    19261 agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg ggaacattac
    19321 caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg gatacagcag
    19381 attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc gagtctacga
    19441 tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca aatttgaaaa
    19501 attatgaagg ccacacattg atcacaacag atggcagtac attactggga gcagatgaca
    19561 aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat ccttctatcc
    19621 ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc ggagcggata
    19681 aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt ggtcctttag
    19741 gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc caagggaaaa
    19801 atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta gcaatcgatt
    19861 ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac gaaggtttct
    19921 tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat atcattcgcg
    19981 atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc caagaagagt
    20041 tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa tattacaaca
    20101 tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa gcaatgatcg
    20161 agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt tcaaaaatct
    20221 cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg cacggacgat
    20281 tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta aaaattgcag
    20341 aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg ttcgtcccaa
    20401 tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt tgtcccgccc
    20461 tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt tttataacaa
    20521 aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt atgtaaaaga
    20581 aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg atgatgcgga
    20641 attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag gtggagacgg
    20701 cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc atgtcggatt
    20761 acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc tcaaaaaaga
    20821 agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg gtcaagtcaa
    20881 tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag cagtttggat
    20941 cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt tttttaagtt
    21001 attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc attggaaagg
    21061 aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta caaattttga
    21121 cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt ccttagatat
    21181 tttccgattt attttttatt tgcctagaat catgagaggc aaattctata cgatccccgg
    21241 cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat ctgtgggcac
    21301 tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg tatcaaaagg
    21361 attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt taacagaaag
    21421 ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac cttttcattg
    21481 tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga actagcggct
    21541 ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt ttgggaggat
    21601 tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact ataggattcg
    21661 ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga gcaggagatg
    21721 tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat tgtgattttt
    21781 gccattattt attatttaat caatcaatca tttgcagata tctttgccga attgctgtct
    21841 accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca aatcgctgaa
    21901 gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac gaaagatggg
    21961 ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac aggaacgctg
    22021 atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg agcaggggtg
    22081 acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt agctggcttg
    22141 gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt tatcatcgct
    22201 ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt cagcttgaat
    22261 gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg tttgcggaaa
    22321 tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca aaccattatc
    22381 caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact tgtattttgg
    22441 tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt tctactgacc
    22501 atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc tgctggtatt
    22561 ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac agttgacgca
    22621 gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct ctatggattt
    22681 gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga agtaaaaaaa
    22741 gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta tcaaatcaaa
    22801 gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta tatacataca
    22861 atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa aactatgtgt
    22921 atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc aaagaaataa
    22981 gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa tgactacgac
    23041 gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac atcaagatat
    23101 cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc gaaatttcta
    23161 tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc tactggatga
    23221 gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc agaatttctt
    23281 tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt ttctttcaac
    23341 ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg tttatttgaa
    23401 taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga gaggaggaga
    23461 aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat acggcgttga
    23521 tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg atgaagaaat
    23581 ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc tgataggaga
    23641 acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg tacgcaaaga
    23701 tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg cattgatcgc
    23761 tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga aagaagtcaa
    23821 aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg taggggcagg
    23881 aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg ctcgcggtga
    23941 attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg aaaaagacaa
    24001 agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg aagatacgat
    24061 cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta acatccatga
    24121 caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc ggttcttacc
    24181 ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag aaatgaactc
    24241 tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa tcgaagaagc
    24301 tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt tgcaagaaga
    24361 attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa cagaaaaaga
    24421 agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac acgaattaga
    24481 agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg gtacgatacc
    24541 tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg agattaagat
    24601 ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat taacgggtat
    24661 ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga atgaaacact
    24721 tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg cggtaattcg
    24781 ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt tcctaggacc
    24841 aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt ttgactcaga
    24901 agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg tgtctcgtct
    24961 agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa cggaagctgt
    25021 cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc atcccgatgt
    25081 cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa aagggcgagt
    25141 agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac agttactgct
    25201 ggaaggcgtg actgctgatg gaacgattcc agaagcggtg gctgaacaag tcaacacgct
    25261 attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga ttttatttac
    25321 accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc aattggcaca
    25381 acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa cgtggattgc
    25441 tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta tcactaagga
    25501 agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta aatcaaaagt
    25561 aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag aagaaatcgt
    25621 ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag tgtcagacct
    25681 ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag ccgaaatttc
    25741 ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact ttttctaatt
    25801 tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac aatcccatta
    25861 aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat gctgctcgtc
    25921 cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct aagaactggt
    25981 cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac gcaaagccga
    26041 ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc aagtaaccaa
    26101 tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag attttgtccc
    26161 aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag atgaacaaga
    26221 aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact ttcaaaatga
    26281 cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat gtgattcctt
    26341 gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca acgaatacac
    26401 caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa agtccctcca
    26461 tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac ttttccgttc
    26521 aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc agctgtttct
    26581 tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc agtatataag
    26641 ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac cccggcatcg
    26701 atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt ttctgtgtca
    26761 gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc ttcattttct
    26821 tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac taactgttgg
    26881 ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt catctaaaca
    26941 tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa tgatacgctc
    27001 tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca aataagaagc
    27061 acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa cggattttcc
    27121 tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag cgaagacacc
    27181 attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag ctgttccaac
    27241 aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa aatatggttc
    27301 gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat ttttataaat
    27361 atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc ataagccatt
    27421 tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt cagctaccca
    27481 gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg ctgtgcgctc
    27541 atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga ataaagcacc
    27601 atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat caactggtaa
    27661 tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc tgattttaaa
    27721 ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga tactgtaagg
    27781 atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa cgataattag
    27841 atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag tcacaccacc
    27901 cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga tttgagagat
    27961 cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg gttttttgga
    28021 acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt cagtcacttg
    28081 gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga ccggttttaa
    28141 ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta ttagcttgat
    28201 aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa aaactcttta
    28261 aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga ggagacgcta
    28321 tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg atcatcgctc
    28381 atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg cacactgtga
    28441 cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt gaacgtggaa
    28501 tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga gaaacatata
    28561 tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc tctcgaagcc
    28621 ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc gaagcacaaa
    28681 cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca gtcatcaata
    28741 aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa gacgtaatcg
    28801 gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg attgaagata
    28861 ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat gcaccgctaa
    28921 aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta aatgtccgga
    28981 tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat ggcaaaacat
    29041 ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac tttttgatgg
    29101 tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca cgagtagggg
    29161 atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac cgaaaaatga
    29221 atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac gatttgcggg
    29281 aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca gaaacctctc
    29341 aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg gatgtcgtcc
    29401 aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca tccgttattt
    29461 accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca gattttcctg
    29521 aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc atggtcccaa
    29581 atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa ttcattacga
    29641 tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt tctgaaattg
    29701 tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca ttggattatg
    29761 aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg aatggagaaa
    29821 aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt ggaaaagcaa
    29881 tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca atccaagcag
    29941 ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa aacgtcttgg
    30001 ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa caaaaagaag
    30061 ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc ttcatggccg
    30121 ttctgaaaat ggatgagcaa gatagtgtga aataacttat aatcaatcga aaaacctatt
    30181 aaataaacct ttttgagtag atgcaacact gaggattatc tgtataaaag attttaaact
    30241 gtcatttggt tatcacggcg atactaaaat taacaaaatt ggaatatttt tcttgaagat
    30301 ttttagctgt cagtatatga tgaaagcatc tcataggagg tgcttttttc atggttgata
    30361 attcaagtgg aagggttctt aaatctctaa gaaaagagaa aaaactatct caaaaaaagt
    30421 tagctgattt agcaggtatt tcacaaagta ctttagttaa atatgaaaaa gggtcaagaa
    30481 aaatccctaa agacgtagat aatactctaa gcaagattct gaatatagaa actctaataa
    30541 aagatgagga agataagatt gagattctca tcggtaaact aattgcatac agagatatga
    30601 ataagttact taacaaagag ttagctgata atataggaat tagtgaagta ttactcagtt
    30661 atgtgttaaa tagaaagaga aatccgtcaa aagaaatgca aaaaaaaatc gatatttttc
    30721 tattaagtaa tgaaaaagaa attttgaagg agattaatcg tgatagtgag atatttagtt
    30781 tatcaaaaga cgataaaatt gttatgggaa aaagaatacg agaagtccga aagaaccggg
    30841 aagagacgct tgaaaaattt ggtaaaaact ttaccatcta tacaggcaaa aatgttataa
    30901 gtagatggga aaaaggtatt aatattccag atattgaaaa actaatgaat attgcctatc
    30961 ttggtaaggt cactgttcct taccttatgt acggagaaga ttataagaat atacttccaa
    31021 aagatgaaag agttagcgat tttaaaaaga taaattcttt tagtatggga ttaaggatga
    31081 ggaaaattag gaaagattat tatctagaaa gggaggagtt tggaaagctt ttttctcctt
    31141 caatttcaaa atggtccata gataggtatg agaatggtag agatataccc aatactaatc
    31201 gaattatcca atatgcttat ataggaaatc tttctttaga atttttgatt tacgggatat
    31261 aaaaatagtt tacgtcgata cttattaagt tatcgacgtt ttttattata gaaaaaattt
    31321 ataaaaggag gtttcttgat atgataagaa acaaaaaggg tttgttgaaa gttgctttac
    31381 tggcagcttt tttctaagta actttttgac aagctcaatg gcaacacaag cggaagaagt
    31441 aatacaagaa ctaactgtta tgaataacag ctagataaaa tgggctatgt atgttatcca
    31501 acaatttagt aatcactaga agcctagcga aagttcgctt gataagttat agatgagtat
    31561 tgatttgtag tgtttccaaa tcaaaagggt acagagccac atgaaatatg ttgtcctctg
    31621 cagcgtcagg atgggaatcc ataccatgga gattcggaga tctgtgaggg aatacttacg
    31681 tggttaatat gtcagttgtg ttggacgtag taatggtcat agtgggtaga ttgactgaat
    31741 tcttatatga gaaaatgaaa ccaacaagaa gatagggtta tttatacaga attggggatc
    31801 ttttctggaa aagtaatcgg ataatgcgag attcgtacgg tcttttttaa atttctctaa
    31861 aaaaaacggc atcgaatggg ttgggtgatt gataagcaat gaatcataga ctgatataga
    31921 ataaaaaatg attctattta tctttttaca tctatcgttt tttcaagtag ccgcaaatta
    31981 tatcagcatt caacaatttt tgtatacaag ttctagtaaa aggatctgaa gaaaaagttg
    32041 gttgttgtga agataaaaaa agaatcaggt tttactgagt ttttcttcaa aatttgtgtt
    32101 attaggttag gacaaagata agaagaagta tgaagattta aaaaaggaat gaggaaaaga
    32161 tagtaatttc cgattattgg agttttttta tttataaaga agaagcgaat aagttgtgat
    32221 tcctagattt cgcgcgtgat taattaatga gcaaaaaatg cggaaagttt tagtgtttaa
    32281 tacacgagga caaagtttct taggtattga aggcacacgt gtaacggttg atgggtggtg
    32341 tgattgtttc gaggttggaa aacatgttga acttatggca tttactgagc taaaagataa
    32401 gaacaacgtg gaatttttcg agagtgatat tggttgggac gatcatgaag aagtttatgg
    32461 aaaatttatt ttgcggaagg gaaattctta tacgaatggg gaagcataag tagcgatttg
    32521 ttagagatca ttgataggat tgaaattgtc gggaatattt atgagaatct agagttattt
    32581 gaggtgatag aatgagtgat ggttttgaac aagtatcaat aattaaaaat caagttcggg
    32641 agattcttag aaaaaaaggc tatttggtag atagttattt tgaaggagat tacgaaacat
    32701 gggtaggtgt ctatgctcgt ccagagaata aacctaccta tttagatcct acgacgtcag
    32761 aagatgccta tttacaaaat aggtatcgag tagatggttt taagcaagac tttgcggagt
    32821 ggtttgaatg ggaaattgaa aacggagaag taaaagaaga ataagagtac ttgttgtatc
    32881 aaaataagcc agtagaacaa ccaaaccagt agctagtaat gacaataata ctgaggaatc
    32941 ggatcattat ggtatgattg atatttctct tccagaagat cttaagccag atcctgtaac
    33001 aattggcaat agtgtcatga tttttgattc attagatgaa gctatgtcat atggttggaa
    33061 agaaattaag gatgaaggta gtaattggta tggcatgaga tttggtgatg gtgaagttgc
    33121 caaaaaagct ggtattccaa ttgggaaatg gacagttgat ttccacgaag caggttcttt
    33181 ttttgttctg taattatagt gggagggtaa aatcattgat gaaatgtgtt cagataagag
    33241 cgccagataa aaatagaaga gacagttcac gtcgttatca cttttggtaa cggcgttttg
    33301 ttttattata taaaattctc aggaaaagga ggaattcttg tgtatgcaaa aacgaaaatg
    33361 aattggttga aagcactcgc attggtggct ttttctcaat tctgtttctt ggtgggaact
    33421 gctcaagcac atgcagaaga aacgacggct atacaaccta cgatggaagc cattgcccaa
    33481 gtcgcttctg aagtgacaga agtccaacca gcagaagccg gggtcccagc tggtgaagcc
    33541 actgttgaac aggcccctgt gacagaagcg ccaacggtca attccaaaac gactctcgat
    33601 cagacgattg gggaaacaca acacccaggg aatagtcctg gtacttcaca accagttgaa
    33661 gaaactgcct caattacaac ccctgcttca gaaacgtcca ctctagaagg agaaaaagct
    33721 gccgaagcaa cgccttctgt gcagccagag tcttctgaga aacaagcgga agaaaagaaa
    33781 cgaatagaag aaacagtgga acaaccagcg gctactgctg acaaagaagg aaaaaatgag
    33841 gaaaacgttg aaaaaacaat ctcttcaacc gcagctagtc aaaatcaacc aacgaaacca
    33901 aacaaagaat ctacggctag tattgaaaat caaaagaaaa aagagcagaa cattccaaaa
    33961 acaggtacac ctacactgtt agtaacagta ccaaccaatc gttcggttcg tgttcgccgt
    34021 gcaaccaatg cagctgtttt aggtggtcgg aaaattgatt ggggcgaaaa tattggtgtt
    34081 acttctagtc cgaacaatct aaacttcact ttaaattata tggttcgttt tgagattaat
    34141 ggacaagtgg cgttttgtat tgagcctggt gtctcagccg cagaaggtca agtttataca
    34201 gctactgctt tgaaaaacta tttgaaaaat tcaaatgtac ggaaaaaaac aagcttgatc
    34261 tcttatttag gatatgtcac aaataaagac aaatccgatg aacaatacat tgcggcacaa
    34321 tttatgattt gggaagcttt gggcacaaaa atcccaatca aaaatatccg tgttgattat
    34381 gatcgtcgaa aagcagaaat tcaacgtttg attaataact ttggtgaaaa agcaactttt
    34441 aataatcaaa cacatacggt aaaaattggc gaaacgttga aagtaaagaa taaaaatgat
    34501 tttttgaaat atgtgaaaga cattcaaaaa ccaaaaggtg tcaatgtatc tattcacggc
    34561 aatgaagtat ggattacagt cacaaaagag gcaccggata aagcaaaaat tcaattgaat
    34621 aaaattaaag ttcaaggtgc gccattagcc tatcgtcgtc caggagctca aacaattggt
    34681 gtgttacatc caacagaacc taatgcgtct gttattaatt tacaaattct aaaacaagga
    34741 aacattcgtg tgtttaaaga agatgcggaa actggtgtga aagcacaagg tgcagcttct
    34801 ttagaaaatg cggtctatgg tttgtatcaa gcagatggga aaaagattaa agaaattacc
    34861 ttgaaaaaaa ttaacggcaa agtacaggca gaaattaaag atcttgatcc tggcaaatat
    34921 gttttaaaag aaatcaaagc gccaaaaggt tatgtgcttt ctgatgaagc aattgaaatt
    34981 gttgttgaac ctggaaaaac tgtcaatgcg accgcaaaag atcaagtgat caaaggaacc
    35041 attgatttag tgaaagttgc taataaagat ttagtcgatt caacgaatcc agataacaaa
    35101 ccaaaattag ctggcattga aatcactttg acgtcaaaaa caactggcaa agtggtgaaa
    35161 accgttgtga cagacaatga tggttatgct acttttggta aaaataccgt tgtctttgat
    35221 acgtacattc tttctgaaac caaaggaaaa gaagggtata aactatttga accacttgaa
    35281 gtcacaattt ctgaaaatgg gcaaactttc cattatgttt tagaagataa agtaattgaa
    35341 caacgtctta aagtggtgaa agtggatcaa gaaacaggaa aagtgattcc acttgctaat
    35401 acagaattca agatttttga tactttagcc aatcgttatg tcacaatgga gattccaaat
    35461 gatacagaaa caacggatat ctttaaaacc aatgaaaaag gctttttcct aactaacggt
    35521 acacttactt atggaaaaga tcgctatcgt ttggaagaaa tcaaagcccc cgtaaattac
    35581 gttttgaatc aaaacccatt agtgttctcg attaccaatg atacagaacc gatcaaagtg
    35641 atccattttg cgaaccgtca agcacggaaa agtgtaaaat tattcaagta tgaagaatgg
    35701 aacaagaaga aaacgccgct cgcaggtgtg aactttacct tgtataacgg taaaaatcaa
    35761 gcaattggtg aatataccac tgatcaaaac ggggaaattt tagtgaaagg tctagttgcc
    35821 gatgactatt acttcttgga aaatgcccca ttagaaagtt tccaaccaaa tacagataaa
    35881 caagcattca cggttgcatt caaacaaaat gcgccagatg atggggaaac gttagtggtt
    35941 tctgtacgta attacttgat tccaccaaaa ctagaaacac aagcgtcaaa taaagccgat
    36001 ggtgaaaaag tggttgatcc attagaaacg atcgtcatta aagacagtgt ttcttatacc
    36061 aacttgtttg tcggaaaaga atacaccatt tcaggagtat tgatgaacaa ggcaacgaat
    36121 gcgccaattt tacaagatgg caaagaaatc acaagtacct tggcctttat tgcagacaaa
    36181 gtcaatggca caaaagacct tgaatttgtg gttaatgcta gtgttctgaa aggtcaaacg
    36241 atcgttgttt ttgaaaaact tttccgtgat ggcattgaag ttgcagcaca tgcggatatc
    36301 aacgatgaag cccaaagtgt gaaagtgaat aatccaaccg ttcataccac ggctgtcttt
    36361 gaagacggat tgacagtcgc agacccaaca ggattgttta ccttaaacga taccgttgaa
    36421 attaccgaga ttcttccagg aaaagcatac ttggtcaaag ggatcttaat ggataaagca
    36481 accggtgaac ccttgcttgt gcatggaaat acggtagaaa cgagtgttgg ctttattgcc
    36541 aaagcagaaa atgaaacggt gatcgtacca tttacctttg atttgacagg tataaatggt
    36601 cgtgaaatcg tcgtgtttga aagtcttttt agaaatgatg aagaactagc aaaccataaa
    36661 gacatcaacg acaaagggca aacggtacga gtaactaacc caagtgtacg tacaacggct
    36721 acacataaac ccactggctt gaaagaggtt aatccattaa cgaaagtcac cattacagac
    36781 gctgtgaaat accacgattt ggtcgttgga aaaacataca cagtcaacgg tgttctaatg
    36841 gacaaagtaa cggaaaaacc agtgctagta gatggaaaag aagtcacaag taccttaacg
    36901 tttgtggcag aaacaacgga tggtacagtc aatctagact ttattctaga cgcacgtgcg
    36961 ttgcgaggca aagaagtcgt ggtctttgaa gatctgttta gagaaggcgt attattggct
    37021 tctcacgcag atattaccga caatgaccaa accatcaaaa tcagtaatcc aaccattacg
    37081 acgaaagcga cgaataaaaa tggcggaaaa gagatcttgg ccttgccaaa tcaaactgtg
    37141 cttgaatggg tcaaagtaga cggtcttgcg attggtcaaa tctatcgctt aattgttcaa
    37201 ggctacttaa caccagacgg cacaccattt gaaggcactc atgcggaaaa agtctttgcg
    37261 gccgataaag aaatgatgga attcttattt gaattcttag tggacggtcg caatcttgca
    37321 ggaaaaggct tgagttttgc ggaatggctc caagcgaaaa cggaagatga gaaagaaacg
    37381 tttgaagaag tggcgaaaca caatgtagat ctaacaaaca aagatcaaac ggttcaaatt
    37441 gtggaagcgc caaaaccacc gcaaccagtg aaaccagaac aacgggttaa accagctact
    37501 gttgtaccaa tgttgccaaa aacaggtgaa caaacaagta gtatacttat ggttcttgga
    37561 ttagtagtat tagtcattgc tttaggtata acactttata aagtacgtaa agccaaagat
    37621 tagtcatgag gaacaagcgg ttaggatttt taaccgcttg tttgattgta atgtagaaat
    37681 atttagaatt tattttggtt tttaagagga ggaaaaactt tatggcagaa aaagcattgc
    37741 gtatgaagaa agagttttta ggtcaagtat ttttattggg ttcacgggaa cctaatccac
    37801 caaaggaaat taatggtgtg tctgggcgat atgttcaatt acaagcagag tgttcgcgag
    37861 aagaacagcc tgcttttttt gtttatctca cagctgaaga ggataaaaga ttacgattac
    37921 cagaaaaaga ggtgcgtatt tcatttgaag aagcttattt tgtttttcgt aaagaacgag
    37981 tgaatagtcg tgaaacaaat gatatgggac aaacagagcg tcgaacaact ggctatcaag
    38041 atgttggtgt ctttttaact ggtgtaaaaa ttggggaggt tttgtaagat gggattaaaa
    38101 tttccaaata attatgacat tacagaagca ttagatgaaa cttgtttagg agccgtacgc
    38161 tttggcgcat tagaagagtc tgatcgtgta tttgataatg aaacaggcga ttatacggga
    38221 gaaattcgat cacagcgagc acaattagtt tttggaaatc cagatgaacc aaagattgca
    38281 cgacgagtta aatttccagc agatgtagat ttgactggtt ttaaacgacg tgatcctgtg
    38341 aaattaattg atccagaata tcatgtggta tatattccag cacaagatgg caatcgtgaa
    38401 tattcagatt tagtgattta tgcgaaagga ttagagaaag tagaagctcc aacaaataat
    38461 cctaatatga aacaatcaca agaaaaaggt ttagatcaaa aacaaccaag tgttgattct
    38521 aaggaaaaag agaaaaaata aggttttttt gaaagaatag gaaggagtct tcctattctt
    38581 tttatctaag atagaaggaa ggggagattt tgaattcaga taaaaccaat caaatacttt
    38641 gagagattgt gcttgttatc aaaatatcca acaattgatt gagtattttg gagacaatcc
    38701 aacgattttt gtacaaacaa atgcatatat ttgtgcggtt attgggcagt gtatgttttt
    38761 acgtgatctt gttcgttttt cattaccgta ccagtggaaa caagatcatc aggaagtgat
    38821 ttggattcgt atagtacgaa tatgtaatat gtgtgcacac ccttataaaa atcgattaaa
    38881 tctttttcga ttatgttttt ttactaaatg tgatattcct gtattagttg ctttttgtca
    38941 aaagttttta gcacaggaaa agtaaaaaca gaaaggagga atgtgacaat tgaaagctca
    39001 ttttttcaag tatcgtggtc ggcgaattcg ctatttcaat cgttatttat ggttttgcta
    39061 ttatggtttc tttttgtttc catttttgat tctgttaggt tggtttagtt ggaaagtatt
    39121 ttatccacgt ttaagtacgt tttcgaattg gcaaatgtat cttattcctg gaataagtat
    39181 tgtgttgtat ttgttgctgg ttagtgggtt aattttaggg ttgatgcatt ggtcgcgact
    39241 aaaagaagga tattttgcct ctgtttattg gcgtcagtta cttgttcgaa tgttaattga
    39301 caatcggttt gtttatacaa agaaacaagt tagtgagaaa caaacaaggg aaaaaatgag
    39361 attaccaaaa gtttattttt atcaaaagaa acaagaatta gtggtttctt ttcctttaga
    39421 tggtgggcgt tttcacgaac gatttttaaa aatgggtcat ttgttatctg aagtattttt
    39481 gcgtgattta attcgtgaag aacgagaaaa agcccgaatc aattatgtat tcctttcaga
    39541 ttctggtcgg attcctattg atcaatgtat agcagaaaat ggacgtattc atttaatgaa
    39601 aactttggat tgggtatatg atcaatcacc aaatatgttg attgctggtg caatcggtgg
    39661 tgggaaaacc tatttattgt attctttaat tcaagcttgt ttaagtgttg ggacagtaga
    39721 tatttgcgat ggaaaagcgg cagatttagc agcattaggt gacgtcgatg tattcaaagg
    39781 acatgtgttt tacaaaataa atgaagaaat gattatttgt ttacgaaatg cattaaaaga
    39841 aatgaatcaa cggtatgcct atatgaaaac catgaaagat cctaaatatg aaccagggaa
    39901 aaattatgct tattatgggt taccaccgca ttttatcttt tttgatgaat gggcagcttt
    39961 ttatggttcg ttggatatgg aagcgagaaa tagagtcgat aaattgattc agccattagt
    40021 attaaagggt cgtcaagcag ggatgtacat tattttagca atgcaacgac cagatgcaga
    40081 atattttcca agtggtgttc gggataattt aacattccgt atttctgttg gtcgattgtc
    40141 tcccattggt tatttaatga cttttggaga agattttaaa aataagccat tttacaacaa
    40201 agatcaacct ggtcgtggat atgcttttcc aggatttgat gtacctgtag agttttttgc
    40261 gccatttgtt tacaaagatt atcactttat caaagaattt gagaaattta ctgaaatgat
    40321 tgtctatgat tttacagtaa aagaggatag tcaagatcaa acgatgggag atgataatca
    40381 gttgttacaa gaacaactag agcgccaaat gtatttagca aaagatccaa aaggaacaag
    40441 tttttctgat gcgcatattt tgtaatcaac taaggaggtg aagttaaatg gcagagagtt
    40501 gttattatag agtagctaca gcaatacgaa tgattaatcc ttctctttct tccagaacgt
    40561 tttatgattg gttgaatcgt attgaacaag taactgatta ccgctttttg cgcaaagaac
    40621 gtgtttttac aggaaaagtt attaatcaag tattgctaac gaaaaaagat atagaacgat
    40681 tgactcgatt gtatcattat cgagtagatt tagaagagga tttaactttg tcaatttatc
    40741 gcgttttttc tcctgaaaaa tatagtgaaa taacaaaatt agatcattta attttgtagt
    40801 tattatctat cgggagggct ctagacgtag tctggtagcc gcactctgcc tggcggtgcg
    40861 cattaaggct gcgaagcatg ggcctataat gcgcactcgc tagcagcgtg tgcagctcac
    40921 aaagaggtcg tgcgcttgcg cacggcctct ttgtgtaaag ccccccgata tataatatgg
    40981 ggggcagaaa taacataaca catagaaaaa agttttataa acccttttgt atcaagtgat
    41041 tatctaaggt cagcagttgt actacttttc ccaaaaaacg agggggacta gttgatatat
    41101 cggtgtttct aaagggttaa aaaaaatcga ttttgttatt ttataaaagg agcgagaaaa
    41161 tgacaccgat tgagctaaaa agctacagaa agaagttaag tgcaattcat aatataagtg
    41221 gagataaaaa gccgctatca caagcaagat ttgcgcaatt gatgggctgc tcaaaagatt
    41281 acattagaaa gatggaacaa gggaaaagaa aaatcccaga ggattttgaa caacgaatca
    41341 aggaacgctt tcgattgtat atgaaaagcg gaatagatct tcgagcaatg attgattatt
    41401 tgcgtctttc cttttttggg acagactaca aaacagtgat tcaacgtgta ttacgaatgg
    41461 atctgacaga atttgaaaca cgtccaacgg gcttgtatct ttttgactgt ctttctgttc
    41521 gtgggaatat ctgggtgttc tggcattcaa aacgaactgt aacagaaaat gtacttgtgc
    41581 aattttcagg acaaggttgc cgggaatatg aggaagtcat gcgtgaacaa caaacagatt
    41641 ggcgaacatg gctatattcc atttggaaaa atcaaggaaa gttggaagct ggttatacac
    41701 gtgtgcaatg tagccgattc gatattgcgt tagatgaact ttggggattg gaaacggaag
    41761 aacatttcga tctctttgaa ttactagaaa aacaaaaagc aggtttatta gaaatggact
    41821 ttaagagatt ccagaatatt ggcggttgtt ttttagatga tggctatttt cgaagtgaag
    41881 gattatcttt gtattttgga tcgcgaaaga gtcctctctt ttttaatttt tatgagaaac
    41941 gttttgaaat tgcgaaccga gaaaaaattt cagaattaga agcattaacg aaatatggta
    42001 tttataatcg atatgagtta cggttagcca atgagaaagc aacacaagca gtagaagcat
    42061 ttattttagg tgattcaccg aaacgtttag gagagattgg cgttggttta attaatgcaa
    42121 aacttattgt ttgggataga ctggaaagtg atgcacggat tgttgataaa cgatgggcaa
    42181 aattatttcg ttcgatggaa gaattggatt ttacagtaaa gccagaaaaa ttctcattag
    42241 atcgttcaga acgctggttt ttgaaacaag tagctccgac attaaagttg ttacaagaag
    42301 cagatcgtat tttaggaaga gactttgtac aagaagggat tgataatgcc gaactaaatg
    42361 acgagcaaca aaactatcta gaatggatgg gaacgtcagg agggaaaatt gcatgaatgc
    42421 atatacgaca acagaatggc tgcgcctgtt ggatttaaag gctgcaatcg aagcattaaa
    42481 tgaaaagatg gttgatttgt cttattttcg ttttcgagtt ccttatattg aacaagcggt
    42541 aaaagctgga cgttatcagg aaaaagagaa ctggcaagaa attgcacgct tattagaagt
    42601 tcgtaaggga tacgaacaag aactcgaaga gttggaattt agccgaagga aaggaaggct
    42661 agaattcatt cgcttttatc gattttcatt gccaatacct gcgattttag cggttaaaaa
    42721 aggctgcgat aaaatgaaga tatatgaaaa ctgtgtggca gcacttagca gcgaaaaacc
    42781 tcttatggaa gaaatttcat tggtgactgt cacttgggaa atgagtcgcc agccaacaga
    42841 agaacagaca tatctttctt tagaagagat tgagatagaa ttagaggaaa ttggtcgcta
    42901 tgccacttgt tctacctatt gtggatcggt cattagtatt gctggtgtga ttgtctaaat
    42961 gaaaaaaatt atgatttatg ttggtgcgta ttggtcacgt gatccaacag tattagaaaa
    43021 tccagaggcg gtacactatt gcttgcgcca attattctat ctgtataaag aacgattaga
    43081 atcgttgatt cgtcagttac catatactga cagacgttta gatgaacttc ttctaaggta
    43141 tccagcaatg tataaacgac gaaaaaatag attgttacca gaagagtatc ccattgaaaa
    43201 acatgaactt gaagggcgat ttgtggctta tttctatgat gatgttcgta tgcgcttagt
    43261 agaacaacga atggaaatag aaaatgatcg ctactatttt atcaattatt gtaagaagcg
    43321 taagtatcaa gtaactgatg atttttatga ttgtatttta caagatgggg aagtgattct
    43381 atccaaaatt gctccaagtt ttcgtgaact tgtgccgatt gattttttga aaaaatgtca
    43441 tttgcggatt cttccgtaaa aaagagctat aaaaggaaag aaaagtagaa gccaaatcgg
    43501 tttctgcttt tttatttttt ttaggaagga gaagatggta tgtcactgga atttttagga
    43561 gagccaattg aaaaggaaga gttcgtttcg cattatatgg atttgtttat tcaggtgata
    43621 aaaaactttg tgcgattgat gaattttatc cacgcgaaca agactatcta gccgctgaac
    43681 gaataaaaat cagtttatta tgggaaaaat tcacagatga aaataaccga ccgccagatt
    43741 atcgctattt atcagattgt ttaacgaata atgtgctttc taaagaatac ctatttcaca
    43801 aacgtgagaa aaatcaaatg tcggttgaac atttttgtga ttgttatatt gcattattgc
    43861 gtagtcaaca gttattaaca gtagaacgat atggtgcaga gtttggttat gtcatgcaag
    43921 ctgagcgtca tagagctaca agatgctatg aaaaagaaga tcaagtgatt gatggctatg
    43981 aagaattatg tattcgaaac aatcgctatt tgcaacaacg tttatatgaa caattgatga
    44041 tggatttttc aaatctctat agagggtatg aatgttcgat gattcaagaa aggtagaaga
    44101 aaagggggaa tttttatgtg gcgattatta tttttggtgg tatgtggtat ggcattgttc
    44161 tatgtgtatc gtgtctatct ctatccacga atggaacgat ggacaagttg gtatagagat
    44221 cctttaaaac aacgaaaagg attgaagcgg tatcgttatg aattacttgt tgaattttcg
    44281 gatgggcgac aagcacagta ttattttttt gcaaactatc caaaatacaa caccagtgaa
    44341 ctattgattc aattattcaa aaataatcca tatgttgggt tagaagaaaa cggacgggag
    44401 ttcttttatc gttcagataa tattagtgcg gcgtctatga acaaaacacg aattgcataa
    44461 aaaattggag gaataatcat gaaaaaaaca acattagcag tcgtaacaac actaggaatt
    44521 tcattatttt cgtttcaatt agggagttca ttggtttatg cagatgaaac tgtagaaaca
    44581 cctaagtcag aaattgtaga aacattgcca ggggaaggat taccagatgt tgagacagag
    44641 ctaaatggag gaacaacgga aaaaccagca gaacctgaac aattgcagtc agagaaacct
    44701 gaaaaagagc cagtgacacc agaaccagag tttcctgaat caactttacc gactgattcg
    44761 atcgaagaag aaggtgtgca tagttcaaca acaacggatt caagtatagt cacaactccg
    44821 gaacaatcag gaacaaccag ttcttctgaa aagaatactg attcgtcaag tacggaacag
    44881 ccaacgatgt cgtctaccag tgaagaacaa aaagaagaac cgaaacaacc tgttgaaaaa
    44941 tcagaagcaa ttccaaaaga aaataaacct attgttccag caaaagaagt cacagtatcg
    45001 gtaaccccaa gcggtgaaat tacgacgaat acttctcaag gaatgagtgt accgattgtc
    45061 actagtaatg tagaggaatt gacccatatt ccaacaccaa ccaccccatt aaaagtagca
    45121 ggtgatcaaa caattgttgg tgtaaaagat ggtattccat taatccaaga tagtgaaggg
    45181 aatttggtaa aggatttgtc gattcctgta aagaaacttc caagtggcaa tattgaaatc
    45241 aaaacagcag atggaaaaac aaaagtctta ccaaagactg gtgaagaaat acatgttgca
    45301 ctttctgttc taggaggagt gttgacaagt attgctggat ttgttggata taaaagaaag
    45361 agaaaaggaa tagaaaatga gggtaattga atggactatt tttcagaaaa acaagcttat
    45421 cgttgtcaat cgtacgtact agtaaaaaca tactagaagg agtagcttat gaaaaaacga
    45481 caactacggc ggaaagaaaa agtttataca gagaaagtac cgaagcaaaa gaaaatccat
    45541 tcaaaaaaag cagtgaaatg gatttggtgt ttggttttat ttctttgttt ttcagggggc
    45601 ttggcatttt ttcaatccat gaaaacgaag gttcaagtgt cgtttttgaa agatgaaatc
    45661 caacaattac aaaagaaaga agtacaaagt cggtcagaat taacgtacac acctgaaatt
    45721 gaatccttta tgaatcgttt tgttgctttg tatatgaata cattcgataa tcatgaacaa
    45781 gaacaacaac gtcaagaaac attaaaaaaa gagttttatg cacaaaactt tattcaagaa
    45841 ccaggaagtg tttcaggtgt tcgaaaatta actgctgctt cgtttattgg attaaaagaa
    45901 attgaaacca ttccaactgc tagttataaa gtaacttatg atattcgctc gatgaatcca
    45961 gagagtaaga aagaaacaag tgtgacaatc acacaagtac tcaatcttcc cttttctatt
    46021 tcaaacaagg gctgtcgtat tattgcttat ccttatttta cggcactacc aaagaataca
    46081 gaaaaacaac gaatgattac ttatgatgac acattatacg aaacggttga tgcgtcggaa
    46141 gcgaaagatg ttgaaggatt tgtggaagat ttcttagata agtatgcgaa aaacaatgca
    46201 aaagatatgt catacatgat gaaagaacct gaaggactca atcaaacagc taaagttgat
    46261 caagtagaga tgaaagtatt gcgttataaa gaaacgaaaa aaccttttat tgtaaaagca
    46321 acggtcgtct ttcgagatgg tgatacgtca cttttacaaa aagaacaaat gacgattgtc
    46381 attacgaaaa aaacaaatca gtattatgtt gaaaaattaa cacataattg gttggatagg
    46441 aaaggagaag aacggaaatg acactgaagg gaattgtaga ttttatatta acgcaaggtg
    46501 gctatttttt aataatggtg gttgtttatc tgggattgcg aaattttatg aaatcaaaag
    46561 taggaaatgc attaatatct atcgcagcag gtgccgcagt ttatttcttc ttaaatgatc
    46621 caacacgtgt atttgatgcg tttggtaaaa tcattgaaaa attatttatg gattaggagg
    46681 atagtgaagt ggattgggaa ccttatgatt attcgattgt ttatgcggaa ccttatcgcg
    46741 tacaacagat tacaagcaat tggcgtcttc catttccagt tgcattagtc aatgcagcaa
    46801 cttttttgtt ggtcgtaatc tttgaatatt tatttttacg ttcatttatt ggttgggtca
    46861 tttctttttt tccaccagca ggagttgttt tttatatagg actcccctat ttatgtgtgt
    46921 tagcactggc aaaactaaaa cctgatggaa aaaaagtgca tttttattta tgggattggt
    46981 tttgttatat gattcgtatg atgaatggtg attgtgtgta ttataactgg gacaaggtat
    47041 caaacaatgc attggaagaa ttactttttg tgaataagta tgtttattta gaggattaga
    47101 aaaagggaca atcaactggt tgttcctttt ttattgggag gtgaaattca ttggtgcttg
    47161 taaaagaacc agtaaaagca ttcaagaaaa attgtttgtt aaatggagag gattgtatct
    47221 gggcctacta tcatgttttt ccacgaacaa tttcaaaaaa cacaccaaaa aagacacagg
    47281 aaataaaaga aatgatggct cgtttactgt tgcttttagc agattatgaa gatgtggagt
    47341 tggtgatgta tccacgaaca atgaatttac ggacgaagtt tgacacatta ggaaagggat
    47401 tttctcccga tagtcaagca attggtgcgt attatgcaaa agaaacagtg acattgttgg
    47461 aacgagaagg tcaagtctat cgtccttcat ttattttagg aatcaaagtc aaagaaaaag
    47521 caacggtgga gagttttcat gatgtaataa atctaatctg gttagattta gcaggaagtt
    47581 tagcacgctt atttggatta gaagtagatg atttggaaac tttgttttct gaaagtgatc
    47641 cttatgtaaa agaagtgaca caattacttt atagcttttc tattcgtccc ttagtaaaaa
    47701 aagagctgtt gtatctcaat cgtctgcaat atattcgagg aatggctcac aaagtagaag
    47761 aacaaaataa ttgggaagta ctgaaagaca atcaaggaac gtatgtacgt ataccgaagg
    47821 aacataaagg ccttcttcaa ttagaagatg agttagggag tagttattta gcattgttgc
    47881 ctattgggga aacacctgaa aatatgagaa attgtgatct atttcaattt gctcaggatc
    47941 tttctttccc agcagaattg cgaatgaaaa ttcactatcc acgcttgaaa ggacagggag
    48001 gtattggttt aaaactgaat tggctatcgg aacggttcaa aacagagcat aaggaattgg
    48061 ttcaaaatga tgaagaacct agtgaacgct tattaactgt tcgaggatta gtaaaacatt
    48121 taaagcaacg agtagataat gggcaacctg tcgtcgattg gatcgcatgt tttattgtat
    48181 acggtcaaac ggttgaagaa gttcgtcaaa atagtgcaac ggttattcaa caacttcata
    48241 cgattatcca tatccaccgt gcaaaaaatg atcaacgaga attgttctat aaatgtttat
    48301 tagggcaacc attgggacgg cagttacatt ggaagcaccg aacaactgta gaagcagttg
    48361 gcgaatggtt gttttcagca gtatcagaat taggtatgga atccggctgg tatattggtc
    48421 ggcaagatac attaggggat ggtggttctc gaggagaaaa aacaccatta gaagaattaa
    48481 tttatgcgtc aaatcgtttt gtctttttga atcttctagc cattgcggaa ggaatcaaag
    48541 gtgcgttgtt tgatgcgcct catgtagcag taactggaaa aaccggtaaa gggaagacct
    48601 ttttagttgg cttgctcttt ttgtatagtt cgtttatgaa tgtacaaagt ttatttgtgg
    48661 atcccaaagg agaaaagcgc aaatggttta cgaaactcat tcaagacccg tattatcaga
    48721 aacattaccc tttgtttgta gaacatttgc aaaaaatgag ttatgtcacg ttagatgcta
    48781 caaatccccg taattatggt gtattagatc caattgtttg tttatcccga gtagatgcga
    48841 aacaagtagc ccaggatatg attgatgagt taagtccgtt aggtaccaat caccttttaa
    48901 aaggggcggt attaaaggga atccaagaag ttttagttca aaaagaagcc ggacaacaag
    48961 ttggcttgat gcatgtggtc gactatttag aacaaatgga acaaaaagag gttcgagaat
    49021 atggtgagtt tttacgcttg acggtagaag attcgatttt acgtttagga ttttcttatg
    49081 gagaaaaccc gggattagac tttaatgcca aaacaacgat tttagagatc caagatttga
    49141 aattaccaaa tgacaatgtc cgaccagaac tatatacaga tgcggatcga aagtctttgt
    49201 gtttgatgat tagtttaggt cgtttttgtg aaatgtttgg aaaacgagat agtagtaaga
    49261 aaacagcgat ttattttaca gaagcatggg tatttaataa ttcgaacgca ggacgtagta
    49321 ttattgcagc aatggcacgt gtggggcgtt cccagatgaa tcaattagtg ttagatacac
    49381 aatttattgg tgacttaggt tcagataaag aaaaagggaa ttttggtgtc gtctttgctt
    49441 ttgatgaaga cagtgagcga gaaaagattt tgaatcattt acgtttggaa gtaaatgaag
    49501 cgaacttagc tgaaatgagc cacatgatta aaggacagtg ttggatgtta gatccctatg
    49561 gacgtgtagg aaaactcaat gtacacagtt tgtttgaaga aatgaccgct gcattacaaa
    49621 caacggaaaa aacaagtcac tctcaagtag aagaacagga tacgttaatc atgcaataga
    49681 aatgaggtga aacaatggct tatataaagc ataaggaaaa gaaatggtgc gttttgtttt
    49741 ttttaacgat tggattcgga ctgctttttg ttggtggatt ggtgtatgcg gcagaaaagc
    49801 aggtggatca atcgatctat gagattggga aatttcatat tgataccttt tcttcttatt
    49861 tatctgatag tgaattttca ttaggcaaaa tgttatttgg ggcaaatggc acagattttc
    49921 taaaaatgat gacgaatatg ttctttagtt taagtaaatt gatttggcaa ggccttgatt
    49981 ttgtcattga gaagctctat gaaggagctg caatgaacga gttaattcac cagtttttca
    50041 ctttttcgga tactatttat tcaaaaatat acgatcctat tggtgcgggg attgtcgtct
    50101 tgtatgtgtt atatctattt actcttcgta tatttagagg gaaacattac gcaaaaatgg
    50161 tgtttattcg atttctcttc gttgtcattt tttcaatggt ttggtttggc tatggtagta
    50221 acgtacctag taaaggagaa gcttttacca aagggttaaa tgatttgtcc gtggaggtag
    50281 aaggctttat tttttctgct acaaatagtc tagatgaatt ggcgacaaaa gatgtcaatg
    50341 gtgttttagt gccaacgaca caaaaggaag ccattcaaaa agtgcgccaa ctttattata
    50401 aagcggctgt tgttgatccg tatatattgt taaattatgg gacaacaaat ttagaagaat
    50461 tgtatcaagc agagattgaa ccacaagaat ttctagccaa ggaagcgtca agtgaagaat
    50521 tatctgatat tgagacaaaa gtgaccactg ctgcaggaga agatagaggg aaaaagaagg
    50581 cttatcgtgt ctatattcaa ccaactaaag ctgtttataa agcagtcgtt ggttctatgg
    50641 tgccattgct taatctaagt attggattac cacttgtagt gattggcatg attcgtttta
    50701 tctttcaaat gttggtgttg tgtttattac ttgtgattcc tttttcatta ctcgcttctt
    50761 ttgttccagt atttgattat atgctcatga ataccttaaa gtcctttttg ggcttgatgt
    50821 ttcaaaaatc tttttacagt gtgattattt tggtggcatt tttgatctat aacatagtag
    50881 acgatttaat tccaatgaat actgtttttg gttttttagg aaatatgttt gtcaaagcac
    50941 tcctatcaat tttactcatt gtaaaacgga aagatttatt aaagaaacta gggttaggag
    51001 ctgcagatca aactataaac aacacgaaaa acatagcaag tcattctttg tctcaaatga
    51061 aacaactgcc tggtcaacta tcaactgctg gacaaaattt ggcaatcaaa ggtgcaggat
    51121 tagcagggaa tgtgtatcga gcaacgaatc cactcgcttc tgcagcgata catggatttc
    51181 aaaagtttca acaaaaacgt acaccacaag aatcggtaaa agggaggcaa gtggttaatc
    51241 cctttgaact aggtgggcgt tcaaatcaag ccttgcctta tagtggtcgt catgttcgtt
    51301 atcatggggt agagcctgaa atgtcttcta ccaaatcgaa aacagagtgg aattctggtc
    51361 tttcaaatgg tttgattcca aattcgtcca tgaattcagt aagaccaaaa caacaggtac
    51421 aatcattggc acaattgagt aagcaacaag ggaataatca aagaaaacca ttgttaaata
    51481 aaggtatcgc ttttgttggt caatcgattc cgttgccttc tggaaaagta cccgtgccac
    51541 aattaacagc tgctggttct ctttcacaac agattcgcca agtgaaaaat caggcgaaaa
    51601 atgggagtta tccagcaatg cagcagttat tgaaccaata ctctgttcct aattcgtcgg
    51661 tagccactct atcaggaaat cgttctgtgc aacaaccggg gcaacgttcg tctgaaagta
    51721 aaccgtcgat ttccgcacca aaatcgttac aacctgcatt ttcttcttta gataagccaa
    51781 cagagcaatc ggaaaaaata gaaaaggatc ctaaaacact gccgattttg gacgcaaaac
    51841 caggaactga ttttacttta ccaaaaggac gaacagtcca aaaaccatta tcgaagaaag
    51901 gatgaggaag aaatgaagaa agggtgtcta ggttgtcttc ttttctttat tcttcccttt
    51961 tttctattgc cattcttttt ttcaggagaa ctttctgagc atggaggaga agctacaggt
    52021 ctagctgtcg cttttaatgg ggaatatacc gaaggattac cgattttttc agagattaaa
    52081 ggacgtggtc caatcacaga tgaacatgcg caagcagcag ttggtgcagc tgtaaagtat
    52141 cagttattac cgagtgtgat tctttctcaa ttaggctatg aatcagctta tggacagtca
    52201 caatctggaa gaaacgatac gaactttttt gggattacct ggtttcctgg ttgtccctac
    52261 ccacaaggtt ctgcacgtgg aattggtggt tctgaaggtg gtttctatat gaagtttccg
    52321 aacttaaaag cttgttatga ctattatggc tttatggtgg cgagccaaag caactttaat
    52381 caatgtgtgg gcaataaaga tcctggaagt tgtttattga ttctaggtcg tggaggctat
    52441 gcggcagcag gtattaccga agggagtccg tattatcgag gagcaatgag tatcataacg
    52501 tcctataact taacggaata tgatactttt gcgatcaagc attggaatag cctaccaaca
    52561 aaaaaaactt cgcttattgc aggaaaaagt aaaggcaatg cgaaggtttt ggatcggatc
    52621 attggacgat ccgtaaataa tggggaatgt tatggtggta cggcatggta tgtatctcaa
    52681 ttaggtggcc cacagttaat gggttctgga cacatgtttg cgatgttaat aggggaagat
    52741 tataactgga caacctatgg ttggcaagta atcatgaacc caaaaccaag tgatttacaa
    52801 gcaggagatg tggtgaattg gcaagcaggt ggtgcattgt ctcctggaat ctatggtcac
    52861 accggaatta taacagatgt ttcatatggc ggacaacatt tctctagtgt ggaacagaat
    52921 gccgagcaag gacggattat ggctcggtat caacgcacgt acaatcaaac aaaaattcgt
    52981 agtctggttc gtaaggtgaa ataagaaagg cagtgactag aaacgtgaaa aaagggataa
    53041 ttttagtatt tagttggtta tgtttgatag gtcttactgg ttgtcattgg ttttcttctg
    53101 aaaaacaaaa aacacaattg agaacgacag aagaaacaat cgaagcagta aaagaaacaa
    53161 gtggaactct tcaaagtact gaaagtaaca gtcaaattgg ttcattagaa gacgagacag
    53221 cgcaacaaat gcaaatagat gaaatgacaa aaaatttctt aaaagatttt gctagaaaat
    53281 ggagtaattt cacagatgtt tataaacgca atcaagctgt gagggactat atgacagatc
    53341 gttgtattaa agacaatagt attgatgtag acccgcatgt acaaatgaaa acaactggga
    53401 aactttacca ggtgacacaa gatatagaga atccggatca atatttgtta tttggagaag
    53461 aaacaatgaa taaggttact caatttgtat tacttcaatt gaaagtagat caagaagctc
    53521 aaaagattga tgcgatcaag atttattatg tacggagtgc gtattaaaat aaaaaatgaa
    53581 cactcatatt tattatacac cgaagaaata gaaaaaaaca gactgttttt ttaagaaaaa
    53641 ctatgtcatt ctttacttga aaaaagttgt tataggacag aaaggataaa acaaaaacgg
    53701 aaaaaatatg tcttatgaat aactataaga actattctat tttttaggag atggatacga
    53761 gatgaaaaaa ctaattggct atagtgtgat gtgtattgga tttgttggtg tgggattgtt
    53821 tgtatcgact gaatctgctt ctgcggctga atggtataat cctatctggt ataatccgaa
    53881 cattgaatgt caaaaaggct atccgtatgg accgacaaat ctgaaaaatg gaaaatgtag
    53941 agtaaattgg aatggcgttg tagatagtgt aattactggt tcaatggact ctgctctaca
    54001 tggctttacg ggtcactaga aggaggaaga aaatgtctga agagaaaaaa gaaatcgttg
    54061 aatcattagt agctttaaaa gattctcttt ctaagattga atatgttgat aggcaagaaa
    54121 ttcaaaaact aattgatgat actattattg aaatccaaga tgcgcgttgt gaaggtataa
    54181 aaatttctgt agctttatct aaagtaatag aaaaaatgaa ccgttcatta gcgtttaatg
    54241 gattgaagct agatagacaa actagtctga tatgggatca tttaaaagac ttgtatgaca
    54301 agtctaaaag aagtgaacgg actgctgtat caatcttaaa aggcctttgg ggaatgaatt
    54361 cataaacaag atatttaaaa agtaacttat tttttatgga ggagaaatta tgaaaagaat
    54421 aattaaaata tttggtgttt taggtgtttt cttattaggt ttaagtac
//
LOCUS       DAFGZE010000012        53727 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.012, whole genome shotgun sequence.
ACCESSION   DAFGZE010000012 DAFGZE010000000
VERSION     DAFGZE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.012"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            24..254
                     /locus_tag="LTU54_000713"
     CDS             24..254
                     /locus_tag="LTU54_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL8295447.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(458..823)
                     /locus_tag="LTU54_000714"
     CDS             complement(458..823)
                     /locus_tag="LTU54_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295448.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1031..1513
                     /locus_tag="LTU54_000715"
     CDS             1031..1513
                     /locus_tag="LTU54_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295449.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1710..2600
                     /locus_tag="LTU54_000716"
     CDS             1710..2600
                     /locus_tag="LTU54_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HBL8295450.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2925..5396
                     /locus_tag="LTU54_000717"
     CDS             2925..5396
                     /locus_tag="LTU54_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL8295451.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(5499..7235)
                     /locus_tag="LTU54_000718"
     CDS             complement(5499..7235)
                     /locus_tag="LTU54_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBL8295452.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7232..7936)
                     /locus_tag="LTU54_000719"
     CDS             complement(7232..7936)
                     /locus_tag="LTU54_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295453.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8067..8570
                     /locus_tag="LTU54_000720"
     CDS             8067..8570
                     /locus_tag="LTU54_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBL8295454.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8530..8868
                     /locus_tag="LTU54_000721"
     CDS             8530..8868
                     /locus_tag="LTU54_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBL8295455.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            8889..10307
                     /gene="ffh"
                     /locus_tag="LTU54_000722"
     CDS             8889..10307
                     /gene="ffh"
                     /locus_tag="LTU54_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBL8295456.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10419..10997
                     /locus_tag="LTU54_000723"
     CDS             10419..10997
                     /locus_tag="LTU54_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBL8295457.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11001..11522
                     /locus_tag="LTU54_000724"
     CDS             11001..11522
                     /locus_tag="LTU54_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL8295458.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11601..12155)
                     /locus_tag="LTU54_000725"
     CDS             complement(11601..12155)
                     /locus_tag="LTU54_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBL8295459.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12408..12683
                     /gene="rpsP"
                     /locus_tag="LTU54_000726"
     CDS             12408..12683
                     /gene="rpsP"
                     /locus_tag="LTU54_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBL8295460.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12695..12946
                     /locus_tag="LTU54_000727"
     CDS             12695..12946
                     /locus_tag="LTU54_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBL8295461.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13071..13862
                     /locus_tag="LTU54_000728"
     CDS             13071..13862
                     /locus_tag="LTU54_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295462.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14032..14553
                     /gene="rimM"
                     /locus_tag="LTU54_000729"
     CDS             14032..14553
                     /gene="rimM"
                     /locus_tag="LTU54_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBL8295463.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14543..15304
                     /gene="trmD"
                     /locus_tag="LTU54_000730"
     CDS             14543..15304
                     /gene="trmD"
                     /locus_tag="LTU54_000730"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBL8295464.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15440..15787
                     /gene="rplS"
                     /locus_tag="LTU54_000731"
     CDS             15440..15787
                     /gene="rplS"
                     /locus_tag="LTU54_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBL8295465.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(15919..17055)
                     /locus_tag="LTU54_000732"
     CDS             complement(15919..17055)
                     /locus_tag="LTU54_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8295466.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLHKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTIDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PIDEVVIDYKRESKTIKIKDKFLEDDEYNRLVDFTTSHNKRYSLLFQWLYLTGMRSGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRSHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(17171..17737)
                     /locus_tag="LTU54_000733"
     CDS             complement(17171..17737)
                     /locus_tag="LTU54_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HBL8295467.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWLLAVVVVFIAIGSQGGSD
                     DAKNTVAETTKESVTEVSSAESVAESTVVEEETETTETTIEEVTQEESVPREYRNALS
                     KAESYLGWAGMSEQGLREQLEFEEYPSDAIDYALANVDVDYNEQALAKAESYDDWASM
                     SDSQLYDQLIFEGFTNEQAQYALDNLPQ"
     gene            complement(17873..18526)
                     /locus_tag="LTU54_000734"
     CDS             complement(17873..18526)
                     /locus_tag="LTU54_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295468.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDTTSNQETKISKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQSTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     AQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(18576..19004)
                     /locus_tag="LTU54_000735"
     CDS             complement(18576..19004)
                     /locus_tag="LTU54_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL8295469.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRDRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIEELKNLYGLPESAYRFLI"
     gene            complement(19009..19383)
                     /locus_tag="LTU54_000736"
     CDS             complement(19009..19383)
                     /locus_tag="LTU54_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295470.1"
                     /translation="MSLVTKIKELADEKHVTIAEVERQVGISNGQIRRWDKASPKSEN
                     LKKVADYFGVTTDYLLGNNNVPKWATKEEVVELDKLLDSNVNMSYGGETLTPEQIQRV
                     KDILIATFWDIVKEDKEKGKKM"
     gene            19687..19872
                     /locus_tag="LTU54_000737"
     CDS             19687..19872
                     /locus_tag="LTU54_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295471.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            19885..20133
                     /locus_tag="LTU54_000738"
     CDS             19885..20133
                     /locus_tag="LTU54_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295472.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            20164..20334
                     /locus_tag="LTU54_000739"
     CDS             20164..20334
                     /locus_tag="LTU54_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295473.1"
                     /translation="MGKFNRALVFSAPLIVYALGLWGSRQALIGTIVYMVWIFIGLDE
                     AEYKTKKPVGRD"
     gene            complement(20436..20894)
                     /locus_tag="LTU54_000740"
     CDS             complement(20436..20894)
                     /locus_tag="LTU54_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295474.1"
                     /translation="MIKILSDYQVAITLIISISGFFLSLYNLFKDKRKITLSYFLVKH
                     DRSNRMILTGVIANPSKMPNSIIECTYLYNGKKINCSHYHANGFDMGSIHKSSLLSPI
                     PLAQAISPGYSVAFSEVLDMKDILPGEKLVMVIRTANYQKKFKLKLKDSF"
     gene            21062..21379
                     /locus_tag="LTU54_000741"
     CDS             21062..21379
                     /locus_tag="LTU54_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295475.1"
                     /translation="MLIATDTLDRIFLKDEYKLRNIDASGILVFDLYDNGKIGIYQAS
                     DIEETNLAFEQIDDSVELDLDEAILAFEQIAKLLKEAQKNGNSLPTQRVIYQSPGTGR
                     TIG"
     gene            21312..21791
                     /locus_tag="LTU54_000742"
     CDS             21312..21791
                     /locus_tag="LTU54_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus Gp157 family protein"
                     /protein_id="HBL8295476.1"
                     /translation="MATLYQLSESYIKVLELAEQLDEEILRDTLDSINEAIEYKAENL
                     AKIVKEVEGKAELIDSEIKRLQERKTSLLNNAKSIKHYLQEEMEKTGKTKIKGELFNI
                     GIQNNPVSVNVINENLIPKGFFTPVPPKLDKKQLKEELKHGDIPGAELVQTKGLRIR"
     gene            21808..21984
                     /locus_tag="LTU54_000743"
     CDS             21808..21984
                     /locus_tag="LTU54_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295477.1"
                     /translation="MEHENVKDALKAAIEIAEAKGIKVDGKPATVHDIQNLTKEHLYF
                     IADLLGLSELYLDK"
     gene            22241..22672
                     /locus_tag="LTU54_000744"
     CDS             22241..22672
                     /locus_tag="LTU54_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295478.1"
                     /translation="MKNKLAKTTAIIGLALGSGVIGYAASNAFQDLDTIKANFNTVLQ
                     YGQTKSQRVSELESQLSNNTRTQEQLKAEIEQIKSDKQKEIEAKQREVEQKQQEIATK
                     QQEADSLRQQLSTVQNDKEQLEQRVSELRQYTDQKVGELGK"
     gene            22678..22995
                     /locus_tag="LTU54_000745"
     CDS             22678..22995
                     /locus_tag="LTU54_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HBL8295479.1"
                     /translation="MTIYDPNISWAKHTIKVSFMIWGYKGYVTYKVGGNTKGLSLIAI
                     DSDDLYDANFEDNPVNFRDLDEDWFSMELTNDKGDSTLVEDEFDRLGDYIVGVEIIAH
                     EPE"
     gene            23114..23800
                     /locus_tag="LTU54_000746"
     CDS             23114..23800
                     /locus_tag="LTU54_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295480.1"
                     /translation="MQIKKATDIEQTKGTYLIYGAPGKGKTSTAKYFPGKTLILDIDR
                     TSKVLRGEKNVDIVYIDNEDTWNDWGNTLADLTTNYVGVYDNVVVDNVSELERCILSS
                     LGAEGKNNGVPSQGDYQYMQFRMVNSLRYMKNLDSNLIWTAWEEIDLWTDSDGSSYNI
                     ALPQINRKIRNNILGLCDVVGRLMVKEDDERGFILQATDSTYAKNQLDNRSGCKQSEL
                     ILDGIVRLPE"
     gene            23775..25124
                     /locus_tag="LTU54_000747"
     CDS             23775..25124
                     /locus_tag="LTU54_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBL8295481.1"
                     /translation="MELYDYQNDLVDRARQAYVDGYKAPCIVSPCGSGKSVMVAEIAK
                     RTTRKGNRVLFLVHRRELIDQIKDTFQKMGVDNQLVNFGMVQTVVRHLKTIKKPQLII
                     TDENHHGLAASYRKIYEYYSDVPRLGFTATPIRLNGSGLGDVNDLLIEGVSAKWLIEN
                     HRLAPYEYYAPKLIDTAELKKASTGDFTKKSMDKAVKNTIYGDVLKHYKTLAEGEQAI
                     AYCHSIDASKHTAEIFNDAGYKATHIDAKTPKDERADIIEAFRNHEIKILCNVDLIGE
                     GFDVPDCSTVIMLRPTQSLSLYIQQSMRGMRYRPDKTSIIIDHVGNVNRFGLPDMDRE
                     WSLDTKKKTKTDSDLSIVQCAFCFGAYERPKGDNICPYCGELQPIEERKSELEIDESA
                     ELMKVGETKITLNFENNKYYNMTEDEAESIEDLYAIARAKGFKPGWAYMAAKRKGWL"
     gene            25115..25588
                     /locus_tag="LTU54_000748"
     CDS             25115..25588
                     /locus_tag="LTU54_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8295482.1"
                     /translation="MVVGMGGIFGKKAEDVQGSIINGVTIIGDTGKRDSSGNQIVIAR
                     NKNNEVFESSLSNIKRGVVNGTGEESGYVNLKKANEKYHVEGTYVKALSVGGTKGVNW
                     DKRRNKWKARLYFKGEYVLQKDFNTKEEAIQARLAAEEKYFKPILEKYENKKEND"
     gene            25563..26090
                     /locus_tag="LTU54_000749"
     CDS             25563..26090
                     /locus_tag="LTU54_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HBL8295483.1"
                     /translation="MKIKRRMINMTGFNLDFSKAQQGNEIKDGTYEVVVNKAVENATK
                     SGAEFIDIDLIVRNDVDQPFQNKHIFAKIWKAKATGKYNEGMIMAIAQALQLEDGKSY
                     NGFDELLADFVLKTASVRVKTEESNGYKNVNVKSWDKTNTRGVMNHQFKNGDEPSFGP
                     ERSRATTVRNDNLPF"
     gene            26105..28417
                     /locus_tag="LTU54_000750"
     CDS             26105..28417
                     /locus_tag="LTU54_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL8295484.1"
                     /translation="MYEKIPKELKNLKQWCVYKLVWDEKRQKHTKIPYNANNGHKAKS
                     NDESTWSDFQTALAAIDHYGMSGLGFFFKPPYFGIDIDNAEGEVERFKSGDIEENIIY
                     EFIESMRSYAEYSQSGTGIHIIARGELPGGRRRKGDVEMYQNGRFFVMTGNAASKYLE
                     ITEPNPKDIKRLYDRYVGDEKIIQFKEENPLMNTVDLPVEEIIKRAESSSQGARFKVF
                     MNGGWEAFYSSQSEADMAFANDLAFWTGRDFEKMDEIFRASAMIRSKYDQKRGSATYG
                     ESLLNKAIADTNSVYNPKRKSDFKIFIKDQEQPKEEKYYSYDDTGNADRFTDIYGTLV
                     KYSYIDKSWYYYDGKVWLQDNTGEVRKMIDTTVDIMGKEPLTIPEGADDETKEALMKA
                     KEKHVKRSRSNAGKNAMMDELKHRLSVLPEEFDKDKTLFNTQSGYLSLHDGLLHEHEI
                     DKMFTRVSNVEYTESVDCPMWEEFINQIFDNDQELIRYIQKCVGYSLTGSTREQCMFI
                     LYGHGSNGKSVFLEIISELMGNYAMTMQAQTIMVKQSQSSANSDIARLKGARLVTSSE
                     PNEGVRLDEGLVKQLTGGDKVTARHLYGKEFEFEPEFKLWLATNHKPIIRGTDDGIWR
                     RLNLIPFTVQIPDHKKDKNLKFKLQTELQGILKWAIDGCLLWQREGLEKPKSVVAASQ
                     DYRNEMDQIGTFIETCCETGPGLKISGGELYKVYREWASDNGEHTFTNTKFGREISKK
                     YSKEKAGGFMVYKGITLKPRKYDNVRELFK"
     gene            28664..28981
                     /locus_tag="LTU54_000751"
     CDS             28664..28981
                     /locus_tag="LTU54_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HBL8295485.1"
                     /translation="MKSEHQIQDEIRIALSKHGCTIFRSNAGQVTTIEGRQFYGMPKG
                     FPDLCGHRNSDGKAIYIEVKNERGKLREDQKRFAEFLNGQPVLYGVARSVEEALKIVE
                     ESS"
     gene            28978..29139
                     /locus_tag="LTU54_000752"
     CDS             28978..29139
                     /locus_tag="LTU54_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL8295486.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            29142..29441
                     /locus_tag="LTU54_000753"
     CDS             29142..29441
                     /locus_tag="LTU54_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295487.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWSEKTKRR
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRAKRENVDSKGRQFKHWEKK"
     gene            29441..29755
                     /locus_tag="LTU54_000754"
     CDS             29441..29755
                     /locus_tag="LTU54_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBL8295488.1"
                     /translation="MEELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EYGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     DE"
     gene            29748..30278
                     /locus_tag="LTU54_000755"
     CDS             29748..30278
                     /locus_tag="LTU54_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL8295489.1"
                     /translation="MNKKVLIDKQALIDELMKIPGVGSNSDALETIKRFPSYEPQKPV
                     VPKFVAELLDYYRQSTDVDLLALLITFHDWYYRKTKDGEHEEAIDWLVDHPEIYMRAW
                     LFGYELEKEPLYCVIINGRYLVKVFSNTNVVILVPADEFSQYVTQKFQLTEKQIKAID
                     ERYWPFAVPVDEVVEG"
     gene            30275..30544
                     /locus_tag="LTU54_000756"
     CDS             30275..30544
                     /locus_tag="LTU54_000756"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295490.1"
                     /translation="MSDPYITKLEKLSEDFEKNKNKLTKDKLIELVRLVIRGAITREK
                     AQQNIEIFARSVRDGLEKKVKQNEQQTPQNNKTKKTGTECTKDKV"
     gene            30474..30677
                     /locus_tag="LTU54_000757"
     CDS             30474..30677
                     /locus_tag="LTU54_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL8295491.1"
                     /translation="MNNRHRRITKLRKRELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASGAIRKFGISILSDDRKWEEKE"
     gene            30674..30961
                     /locus_tag="LTU54_000758"
     CDS             30674..30961
                     /locus_tag="LTU54_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295492.1"
                     /translation="MKLKDGFYSSSHGIGGLMLDMPTKNPKTREKPKFKVGDMVRCEA
                     EGFIYPFCGYVEHLYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMEVI"
     gene            31448..31861
                     /locus_tag="LTU54_000759"
     CDS             31448..31861
                     /locus_tag="LTU54_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL8295493.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            32481..32645
                     /locus_tag="LTU54_000760"
     CDS             32481..32645
                     /locus_tag="LTU54_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295494.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKENNEV
                     ISFRRRRKAK"
     gene            33057..33602
                     /locus_tag="LTU54_000761"
     CDS             33057..33602
                     /locus_tag="LTU54_000761"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295495.1"
                     /translation="MHMHIGEKIYKLRRLNNETMNDLVKQTGVSKSWLSDIESGKKQR
                     VDFNKICAIAKHYKVSLEYFRDDYLDSDEHEDIIPLTGETRVKSVKSLEQSLKYLNNK
                     QEPNLLEIKLKDTDSVPEVWYKGERLDELPKGLVDVSYHWKTDDFTNDDRGANDITIQ
                     YFSSFNDKYPDKKTIGHKRDM"
     gene            33603..33920
                     /locus_tag="LTU54_000762"
     CDS             33603..33920
                     /locus_tag="LTU54_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL8295496.1"
                     /translation="MKEVRPRDEIDKLYKTKRWRDLRQVVIARDFGMCQECKRRGRNT
                     RGTIIHHIVEAREDLSLFWSVDNLECICVACHNREHPERSGGKKKPKPKSHIVKMYST
                     PER"
     gene            complement(34077..34241)
                     /locus_tag="LTU54_000763"
     CDS             complement(34077..34241)
                     /locus_tag="LTU54_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzC family protein"
                     /protein_id="HBL8295497.1"
                     /translation="MGELYKPGEDNKPKGTYKEVGPRGGNVQGGQEVKIDPGDRLPPT
                     SQKGNKWTKK"
     gene            34343..34864
                     /locus_tag="LTU54_000764"
     CDS             34343..34864
                     /locus_tag="LTU54_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBL8295498.1"
                     /translation="MPQPAKSAKLQLLNGNPNKKNTEELRKRAAAEDKLKMATDKIKP
                     PLWLDSLGKDTFEFIADELLSVDLISNPDVHTMALYSNWYSQYVSLEKQLRKLQREYK
                     LNYALAKKEAEARGEPFNEPNELIGNPLSRQMDTASRNLRSFGADLGLSPAARAKLAI
                     KMADDGGDDDDDF"
     gene            34851..35324
                     /locus_tag="LTU54_000765"
     CDS             34851..35324
                     /locus_tag="LTU54_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL8295499.1"
                     /translation="MTTSNILDMSYTERVDYWQSYLEEQASWGGFLKCPYPELLTTWY
                     AERLIDGSIPASKENIQAAKRHMRDLQRQGTDDFPWIFDEEKGHRPIRYIEKKCKPTE
                     GDFGSFVLQPWQHFIIGSMYGWVHRDTGERRFREALIFVGRKNGKFCRLKIGQNR"
     gene            complement(35331..35489)
                     /locus_tag="LTU54_000766"
     CDS             complement(35331..35489)
                     /locus_tag="LTU54_000766"
                     /inference="COORDINATES: protein motif:HMM:NF024064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HBL8295500.1"
                     /translation="MDKYGLKNRIRISNAIDKDLYEGLKKMSEETMVPMSKLLDKAIE
                     LLLKESQK"
     gene            35582..36343
                     /locus_tag="LTU54_000767"
     CDS             35582..36343
                     /locus_tag="LTU54_000767"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL8295501.1"
                     /translation="MKKYIIYKHLNKINGKIYIGVTNDIGRRWRSGGIEYKPPKNETQ
                     HHRSFWNAIQKYGWENFDHLIIEEDLTMKEAFEKEKFYIELYDSTNKKKGYNIAKGGN
                     GGIIYKVHPKGMLGKKQSKEFSSNHSKWAKNHKNNCMTNGDVVWGVTHEHPKGMLGKH
                     HSKESIMKKKAYSGENAVTSKPIVAIERDGTKREFHSAKLCMAYYSISTCVFYRLLKD
                     GAPYVINPKANYRNKEKILAIEGIMFKHKEDTEVS"
     gene            36563..37882
                     /locus_tag="LTU54_000768"
     CDS             36563..37882
                     /locus_tag="LTU54_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL8295502.1"
                     /translation="MVAYDDEQGANVYVLANARDQASLLFDKAAEMVKQSPALFKKFG
                     KPKRSSINYAPAFSKMEPRASDSRKLDGLNTHFGIFDEIHEFTNYNLINVIKKSRGTR
                     KQPLIVYITTAGYVLDGPLMSYFEQGVDCLEHLEDDIDERTFYYLAKLDSAEEADDPR
                     LWIKANPNICLMNFVGMLDDYVKDKKDPKEYADWITKQFNLFSDIDELSFVDMPTIKR
                     NNKTIDIETLKGKKCVGGFDLSETEDFTAAVLEFPLETGEVFILQHTWIPQARFDRDN
                     NQERIKAWEKVGDLTIIPGDYVNYEYVLNWFVENSKIYDIVKINYDKAKALRLNKELE
                     NAGFETNEIRQGFLSLGGPMQNFKEMLLDGKVIFNNSKLYRWYLSNVKLVMDRNSNWM
                     PSKQSKSRKIDGFAASLNSHAEVLNMLVNPVGTGKVTYYSISDLMNM"
     gene            37898..39118
                     /locus_tag="LTU54_000769"
     CDS             37898..39118
                     /locus_tag="LTU54_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL8295503.1"
                     /translation="MSILDRLRSFGRAKPKASKQEYFLNDPGLIPYLVGKDEISEGIF
                     SVISRVSNVFASLPLKMIDVEFGQPDDCPAYNLLSEGPRYFTKFDFFRDVEVLRNYQG
                     NAYVQIFRNINGEVADMALVKPGACHPVIDMDSGELYYQVTATDKGSYKQVIYVHYME
                     MLHFKQPRFGGLEGTDPTKVLTNTLGYDREVRKISLSQLKGSNEGLKVKFASNMDEEA
                     KKATVKNIADFYRQNGGLLVEENGVEIERLQRELVDSKLLDTDKVSRSRIAMVYNVPE
                     HFIGNNQSSYSSQEQLNMEFLTYNLVPTVNQYEAELNKKTLSRTEKAKGYRYKFNVAS
                     LLRADTQARGQFYQIMRRGGAYSANDVRRFEDLQPINKTGMDDYHISGDLYPIDMDPT
                     LRKTTSSKSVAENG"
     gene            39153..39830
                     /locus_tag="LTU54_000770"
     CDS             39153..39830
                     /locus_tag="LTU54_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL8295504.1"
                     /translation="MKKVTLSGDVVDNDTAWLYDWFGINCISPGKISAALTEAAGDEV
                     ELDISSNGGDVLAASEIYTAIRAYPGKVSGNVVSIAASAASVIACACEPLRISPTAHI
                     MIHNAWVTTSGNAEELKANAEMLSSVDESIVNAYEIKTGLDRKKLADLMAKDTWLNAQ
                     TAVAEGFADEIMFAEAPVTVLNASQPVIPKNAVTKLKNLILKAEAPQEETLLQKKLKA
                     LNGGKNE"
     gene            39827..41020
                     /locus_tag="LTU54_000771"
     CDS             39827..41020
                     /locus_tag="LTU54_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL8295505.1"
                     /translation="MNLEQLKNAWVEAGSKVSDLNAQLNAALVDDEKTEEDVVSLQAQ
                     VKAARAKRDGLKEQVANMEAEQVLNVKKEPLDKKDENLKNKFIKDFKAMVNGDPAIMA
                     TLTSNTDESGNAIGLTIPVDVQTTIHTLVRRFDSLQEYVNVEKVTTTSGSRVYEKWSD
                     ITPLTALDTEDGEIPANDDPALYLIKYLIKRYAGISTVTNSLLKDTAENILAWLSKWI
                     AKKVVVTRNTKILAAIDGIKAAQKKDVTDVDGIKDIVNVQLDPAIEATSMFITNQDGF
                     NVLDKVKRADGSYLLQKDVASATGYTFLGKPIKKIASRFLPNKGTQATPKYPLYIGDL
                     KEAVTLYDRENMSLLTTNIGGGAFETDTTKVRVIDRFDVQLVDDEAVVLATFTTIANE
                     TPAKV"
     gene            41035..41361
                     /locus_tag="LTU54_000772"
     CDS             41035..41361
                     /locus_tag="LTU54_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HBL8295506.1"
                     /translation="MILDPKMDLGEIKNALKIDTDDDDVEVSRAAQAAIAYIRGAIGN
                     DKPSFYKQENETVDLINLAILQLADHYYNARSATVSGNLREYDLGFTSLILQLKASYL
                     LFVEEE"
     gene            41365..41700
                     /locus_tag="LTU54_000773"
     CDS             41365..41700
                     /locus_tag="LTU54_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL8295507.1"
                     /translation="MPLIQTGNLNQRIKFVRDTTVKDEDGQVVPTSTTILTCWASVQT
                     QRLNDIKTSIGTALEGTLTFIIRYQQKSELTNDMKVRWNGKTFEIITITKGEFAKDFT
                     TIIAKEVSK"
     gene            41697..42092
                     /locus_tag="LTU54_000774"
     CDS             41697..42092
                     /locus_tag="LTU54_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295508.1"
                     /translation="MSVEVDATEVYKALREVKANVQRVESPALRKAGEYAQEELRQNT
                     PYWDGTKSNGKRGSYMQEHAKNHVVTSSIKNGLVEVGYDKDVSWRMHFIEFGTIKQRP
                     KGFVQKTQKQIEKQVTQIIADEVKRRLGL"
     gene            42089..42460
                     /locus_tag="LTU54_000775"
     CDS             42089..42460
                     /locus_tag="LTU54_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295509.1"
                     /translation="MKTAVSQVYSILNSNEKTKNIDFYTNSVPESAQTVPSLPVGRIT
                     EISGNYEDFASNNPLTIQFNVQVDVWVSTMKEVDAFYFALDEVMRGNGWQCAYTEQTD
                     DEDLEGAKRIIKRYVANISLN"
     gene            42476..43036
                     /locus_tag="LTU54_000776"
     CDS             42476..43036
                     /locus_tag="LTU54_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8295510.1"
                     /translation="MATVGFESVIFGVKTGAGGTLKELVADKSKGGAIEAKITGLGAT
                     SNTTYASNVPFFIASKGVSSPKVTLDVADLMDNGIYSEIIGAKTVDGANVIGSETEAP
                     YVSVVMVTANKEGKRLFMGLTKGKFSHPDIDMKTAEDKGVELQTDSIEGEFISDERGY
                     VYLTAVESEGMTLQKFKDLVNNKEGE"
     gene            43161..43472
                     /locus_tag="LTU54_000777"
     CDS             43161..43472
                     /locus_tag="LTU54_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295511.1"
                     /translation="MIELQLKLDGKKKTFKQQDISARAMRECIKFYEKAEKADLTDLE
                     AIDSMIAITADIFQDPAVTFDAILDGLTASELVPALESVFEQINELGNNEKKQTASKK
                     K"
     gene            43496..43687
                     /locus_tag="LTU54_000778"
     CDS             43496..43687
                     /locus_tag="LTU54_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295512.1"
                     /translation="MDQIYKDLIEAGWTMRDVDEADYHYLLHLFGEVESGEEYVDGAD
                     FIKQFLSAEDLVKLEEGGK"
     gene            43687..47535
                     /locus_tag="LTU54_000779"
     CDS             43687..47535
                     /locus_tag="LTU54_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL8295513.1"
                     /translation="MAGKGQPAGNIKLGISLDSTSFGNTLDEINAKVKQAESNMRANL
                     KAYDSAGRSYEALSQKTKDLSTVMEGQNAKVRELTKRRDEAISKYGEESKQVANLNTQ
                     INNATAKYNAYSRQLNDTKKELVYSKTAVNDLSNEIKENERQMNAEVKALKAAGDESG
                     AFEAKQKGLAKQTELSEKAIEEQRKVVKLMADEFGDSANETEDAKRALEKLERQSQIS
                     SRQLEALKSSSDQSGKEIEDFGDKSTRSARKLDGLKDKLSSLKSAFSFGAVAGLAHNA
                     ISSVVSGVQGLVGEAVNASDSLMKFSKTMEFANFGKSQIESSKKEMKDYADKTVYGLE
                     EILNTTAQLASNGIPNYTELTKAAGNLNAVAGGSSDTFKSVAMMLTQTAGAGKLTTEN
                     WNQLADAIPGASGLLQDAMLKNGAYTGNFRDAMAQGQITSDEFNQAIVQLGMNDGAVK
                     AATSTDTLSGSWEQMKSTVINGLQSIIEKIGVENITGFINRVTKGIENSIPKITQFIG
                     WLRDIGTWIVENREPLTWIVGIIGGITLAVKALNVASMLLAITGGTLAAPFVAIGVAL
                     GALAGALVIAYTKSETFRNIVNAAFTAVKNVVMSVINNLVEYYKMLWSVLQWLWEKIK
                     EWASWIGNKFIEMKNSVVNTVQNLWNGVKNFFSNGVGDTWNKVVGWVKNIFNKATELK
                     NNVSDVIGNLWNGIKDTFRRGIDTVFNWFSELPTKMKNAIIGGKNAIVDAFKSIFNAA
                     LKAIGKPVNAIIHGASWVLEKLGAEPLQEWDVPQYATGTPAGGHPINGPMMVNDGRGA
                     ETVITPDGRAFIPKGRNVVLNAPKGTHVLTAEETAQLQGSKAPKYRYKKGTNFFGNMW
                     DSVKKVAGNVGHTIKNVVGDVWDFISDPGALARKVLGGLDVLGGLTKYPLEVGKGILS
                     KATSALTEKITGLFSSGNLDTSVGTNGVYKYLADVAKSVMKKFPGFVATSGYRPGDPY
                     SHGKRNAIDIALPGVTGGSPRYTEAANYAFDKFASKIGYVITNGKVRDRSGQSGQPAT
                     GAWEPWPDGDHYDHVHLNGVKDPQNTQISGDSVGGSGVERWRNVAIRALKMTGQYSTA
                     NLNALLNQMRTESNGNPNAVNNWDINAKNGTPSKGLLQVIDPTFRQYAMPGFNSNIFD
                     PLSNILASIRYALSRYGSLTNAYRGVGYANGGIVNQHQIAEIAEGNKPEIIIPLDKAK
                     RSRAMQLLSIAMDKLGVSPKTYGNATTVSSDYEILEAIEKQAATTNQLLSLLLAFFKG
                     NSRTDRDLALDIQKILVRRM"
     gene            47536..48408
                     /locus_tag="LTU54_000780"
     CDS             47536..48408
                     /locus_tag="LTU54_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HBL8295514.1"
                     /translation="MRTVLLRNRINEEIDLSTEDYFATGLSNMGFEVKKEHVGQWGNF
                     RESSESVEISEFQSSVIISVHGFREKELYNSLVQFLSEGPFELEFAFDGETMVRRCSL
                     KSLSKTEIDPKTSLLTDTLELYFTSNWYSVKREKLIQRPNVVKTRGKVFPYKRSYIYT
                     QNLWEKKGVFKFNNNSVYLTNSKERMSPLKIRVIGKCSNPYWEVIQNSQIIATDGYFI
                     DMTETQTLEVSSLFEDTTAILKDIAGVESSVYQQQDYTKTNFVQAPTGEFSIVFHVGG
                     ADVEIELYEERDLF"
     gene            48405..49454
                     /locus_tag="LTU54_000781"
     CDS             48405..49454
                     /locus_tag="LTU54_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295515.1"
                     /translation="MILAVTLFHRDLMLYNEHLFSNSFEFGVDEINEEASSFTMDKYV
                     PVKTGDFLLAKYIPSGKFAYFGVITSQEDEKISCKSLLSLADSEIPTARVSGDNYEEH
                     IRRLIEYYLLNDPTKQLKDILDVKAESATSHSYQATDTNKHKLSAYILNGFKKYNVKW
                     YFKGIQNRKIYTGIRAVNESIYIKDNSSEFSDWDVFVQAPGAGNENKLLIVDKAMKDI
                     EKPIILSTWYLDEENNLTQDGSKENITKPTVNLVNIYDQTAEDKASYEDVAKSELKGN
                     TYSHEIKVNVVRNAKNLNVETIETGMFATISYKGKIYKSVLTAWRISSDKEFVELTFG
                     NIRSRFMDYFEDNGG"
     gene            49458..50282
                     /locus_tag="LTU54_000782"
     CDS             49458..50282
                     /locus_tag="LTU54_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295516.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERV
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            50300..52360
                     /locus_tag="LTU54_000783"
     CDS             50300..52360
                     /locus_tag="LTU54_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HBL8295517.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDQTNQQIGDLGKLKKMYSNSIDFGGYDYSGNPNL
                     LSKLSYDLIENQNTSAGTLSKGENSFKYNKISAEVEGGVELYYKRRGIANWLPSNKTL
                     VMTVKLRAGADYSPVDGKLILIRYRYVDSGTGKIVLDLPINSNSITQEWKEFSITGTT
                     PTFSPQAYHPWIQFRAQDGVLGEIEMSYDIKIEEGSTATPYQPNLLDDSYWLGKTPLG
                     ENLITNAKFPIITRSNPISGFDISEELIIGETYTVSLKGTKPATKEFHLYYGEANYQQ
                     LQYQATLLPVEGLTDVWSATFTARNTSETTNLLRVALWQKPNSATYDTVQIDWLKIEK
                     GDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYTEKSLNNTVSLTEPQSIEGLKNF
                     EDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNGKSSGGN
                     DSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAI
                     TYTI"
     gene            52377..52526
                     /locus_tag="LTU54_000784"
     CDS             52377..52526
                     /locus_tag="LTU54_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295518.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            52523..52969
                     /locus_tag="LTU54_000785"
     CDS             52523..52969
                     /locus_tag="LTU54_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295519.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            52971..53108
                     /locus_tag="LTU54_000786"
     CDS             52971..53108
                     /locus_tag="LTU54_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL8295520.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADRIISKEESA
                     S"
     gene            53143..53385
                     /locus_tag="LTU54_000787"
     CDS             53143..53385
                     /locus_tag="LTU54_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HBL8295521.1"
                     /translation="MKEEALQDVVERLVRIETKLDNYESLREKADSAKDLADKAYSVA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            53401..53598
                     /locus_tag="LTU54_000788"
     CDS             53401..53598
                     /locus_tag="LTU54_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL8295522.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            53609..>53727
                     /locus_tag="LTU54_000789"
     CDS             53609..>53727
                     /locus_tag="LTU54_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL8295523.1"
                     /translation="MKKKIILSLSLIMALFLLPINGFAYTINNEFNLGVNEGSS"
BASE COUNT        18319 a         9189 c        11418 g        14801 t
ORIGIN      
        1 ttgataggag tagaaatgca agtataggtt ttagaactaa acgaagtctg tcttctaaaa
       61 aaaattgggt atactcacaa actatttttt atggaggaat tgttttaatt agtttacttt
      121 cttcaacact ttattctttg aatattatag atgtttccac ctcaaattca atatctatta
      181 ttggaatcat tattgctgca attattacac aactgttttt agtgtttgga gagaagaaaa
      241 gaagtaaaaa atagaaaaac tagtgatagt tttggtgata gtgatattaa aaatcagaaa
      301 agatagaaaa agcaggagat agataaaatg ctgatatttc agcatttttt aagagtataa
      361 aagagaaaaa aagtaataaa tgtatacatg atataagaaa aacatatatg tataaaaaga
      421 agaaagtagc ttctctttgt acatatatgg tttgttttta tggatgccga gcttttttat
      481 aagataaggc taccgcccat aacgacccaa ggatttgtgg aactaagtaa atacctgcaa
      541 gaagcaagat cagctgggtc ggttttaaag gatgatagaa gtaacgccat tcagtaaaat
      601 tggcgcaaaa taaataggaa gtgacagtag atataatatt ggcaatgaat aaagatataa
      661 tacgcctgtt cctttgaaaa taatatccag caaaaagaga taaaaatatc aatgtgatga
      721 acccgcttac ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca taaggaacaa
      781 aagataaaag caacggaagg tttttccagc aagtttttat catctttcat caccacctaa
      841 caagtgttat tattttgtta gttttattgt aaattgaatc ctgcaataat caattacttt
      901 ttcattattt ctagattaaa tgaataaact ctcatgccat tatctctaaa aaaaaagttt
      961 ttccccctaa tttttttaga tagaaaattg tatagtatga tgagagaaaa agtagtttgg
     1021 gggaacgtta atgagaagaa aagcagtaat cgatcggctt tatttatttt tttggttttt
     1081 tcctatcgtt ttgctacctt ttacatgggt aaatgctgtt gtacaaggtg cggagatatt
     1141 aggcctgatc caaggaagcg gatttgtggt ttctaggaat tttccagctt tgactgtttt
     1201 ctatttagtg ataagtgtag gtctcttatt accaaaatct ctgatcaatc actacaattg
     1261 gcagctgctt tacttttctt gcttattagt atttacatct atgttttctt tgctgcctcg
     1321 cttgattatc ggccctaatg gtttgagcga gagtggattg aaggattcag cttattcctc
     1381 catgtatttc ttgacgatca aaccatggtt ctatttagta attgcctcat ttattcttgc
     1441 aacatttttt tacttctgga cagaaatcag gataaaaagt gaacgatctg tatctataaa
     1501 aaataactat tgatggtgga tcatcagtaa aaaagcatat gagctgcgta aaatgcatgt
     1561 ttctcatatg ctttttttat tattatgtaa agaatgcgaa aaacgtttac atgattttta
     1621 cataacttaa ctatattttt acagcaacca tacgagggat ttacagtcga ctgataaact
     1681 ttagttgtaa ataagaaagg agcaataaaa tgaaaaaagt attgtttact ctaggtatga
     1741 tgggaattct tttagccgga tgcggctcag gatcaggaaa tacagatggc tctgccacac
     1801 aaaataattc aagtgactca aatgaacaag taaaaatcgt tgcggtaggt tcgacagcat
     1861 tacagccgtt agtcgatgca gcacaagaaa gctttgtaca agaaaatcca aattatcaaa
     1921 tctctgtaca aggaggagga agcggaactg gtttgagcca agtcgaagct ggagcagtta
     1981 ctatcggaaa ctctgatgta tttgcagaag aaagagacgg agttgatgct tcaaaattag
     2041 tggatcataa agtagcagtg gtcgggatgg cgccaatcgt caataaagat acagatgtca
     2101 aagatatcac caaacaggaa ttgattgata tctttacagg aaaaatcact aactggaagg
     2161 aagttggcgg gaaagatcaa aaaatcaacg tcgtgaaccg tgcaaacggc agcggtaccc
     2221 gcgcgacatt cgaaaaatgg ggactggatg gtgctacccc tgtgcagtcc caagaacaag
     2281 attcatcagg aacagttcgg cagctggtaa gtcaaacacc aggagcaatc agctatttag
     2341 ctttctcata tctcgatgat tccacacaag cactaagtat cgacggtgta gaaccaaaag
     2401 aagagaatgt agcagataac agctggggaa tctggtcata tgaacatatg tacacgaacg
     2461 gaaaaccttc ccctgaagtc caaaaattct tagactacat gatgacagaa gaaatccaag
     2521 aaggacctgt caaagaattg ggctatttac caatcacaat gatggaagta gaacgtgatc
     2581 acgaaggaaa tatcaaataa ctagcagata aacatgccac ggtagtcgtt actattaagg
     2641 aaaaatcaac tatgtttccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2701 atattttgcc gttttttgag ctaataccga gggtcagctg attaaacacc gtttattcga
     2761 aaaaaagagc tacgacatag aactgtcgta gctctttttt tgctatatga caggaatgaa
     2821 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     2881 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     2941 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3001 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3061 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3121 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3181 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3241 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3301 atgtcatacg ggacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3361 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaaggaaaac
     3421 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3481 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3541 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3601 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3661 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3721 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     3781 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     3841 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     3901 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     3961 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4021 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4081 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4141 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4201 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4261 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4321 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4381 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4441 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4501 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4561 ttcaattgat aaagcgtaac aatacagtag ctatgacagg tgatggcgtg aatgatattc
     4621 tagccatgaa agaagcagac tgcagtatag cgatggcttc cggaaatgaa gccgccatgc
     4681 aggcctcaca agtggtctta cttgattctg acttttcacg catgccagaa gttgttgcag
     4741 aaggacggcg cgttgtgaac aatattgagc gatcagccag cttgttttta gtgaagaata
     4801 ttttctcctt tttgctctca ctgttttcag tgatctttgc tctgacctat cctttagaac
     4861 catcacaaat cacattgatt agtttgttta ctatcggact tccatctttc ttgcttgctc
     4921 tagaagaaaa caaaaagcga atcagaggga aattcatcat gaatgtgatg gaaaaagctg
     4981 tgccaggtgg actaacagat atgatcgtag tcggtgcgtt ggtgatctgc ggggtgactt
     5041 tgaatctaaa taaaacagat gtttctacag catctaccat gcttttgatt gcagtagggt
     5101 tcctcgttct ctataaaatc tgttcaccgc tgaataaatt cagaagtcag atcattcttt
     5161 tctgtgctag cggtaacttt ttttcggtca tctttttgca taagctgttt tcaatcaccg
     5221 gtatctcagc tgtatcactt ttgctgttga gcattctatt tttctcggca gattcgattt
     5281 ttaggcattt gaccacattg gtagaatatt tgtttgaacg taaaaatgaa aaagagcaaa
     5341 agccgtttag tcgcgtagct aaaaaatttc tatcgatatt taaacgagaa aattgacgaa
     5401 aagtatctca aaaaagagta aaaaagaaca accactcgtg tatctttcga agcaggatga
     5461 gtcatccaac tttaaaacgc acgagcagtt gctctttttt acattttcgg aatggtaacg
     5521 gtgaaagtcg aacctgttcc aagatgactg tctacagtaa tcgtaccatt aaggagttgc
     5581 acgtagtctt ttacaatcgc taaccctaat ccggtcccgc ctgaatgtcg gcttcgcgcc
     5641 ttgtctacgc ggtaaaaacg ctcaaagatc cgttcctgat cctcaatgtc gatacctata
     5701 ccaaagtctt ggacagaaaa cgatagatca tctgttgcct gatagcggat aataatctcg
     5761 ctgtcgcttt ttgaatattg gacagcattt tcaatcagat ttttgataat tggataaagg
     5821 atatctgttt tagtagtaaa tggttcgttc tttccaatca atcgaatcgt caaccgtttc
     5881 ttttcgatga ttggctgata attttgaacg atttgctgga aataaggttc tgtataaatt
     5941 gtctggatct cgtaagaatt ctcgccatct tttgacagct ggatgatttc ccgaatcagt
     6001 ttatctaacc ggatcgcatc tttttgcatg atctcaagaa aagaagtcag agtttgcggg
     6061 tcatcttttg caccatccag taaagtctcg gtaaaaccaa tcagtgaagt cacaggagtt
     6121 tttaattcgt gagagacgtt gccaacgaaa tccttttgta gcttttctaa acgtcgcact
     6181 ttcgttaagt catacgccac ccctagaatc tgtccggtac cttctgaatt agtaaagtag
     6241 cgcaaactca agtctaatgt ctgccgacct tctgtaaggg tgatctcctg atggatcagc
     6301 ggatgctcag gagtcacttg atgaatcagc tgaatgagtt ttggctcttg aatgacttct
     6361 gtgtaccgct gttcaggttg atagcttacc acgtttaaat gtagctgcat ctttggatta
     6421 agcagtagta aacgagaatc agcatcatca atcaggaata cgccaatcat cagttcgttc
     6481 agtaacgtat ataattgctc ttctgtggta gtacacgccc gatacatctt actcatttgt
     6541 tcgctcaacg cattaatcgt ctgatacaat tcttcccatt gatcgggtgt ttgcatgatt
     6601 acttccgcct gattaggatt ttgcaccatt ttttttagta cgggcagcac ggttttcaat
     6661 ggctggtttt tttgatagat cagataatag atcattcctg caagaacaag gaagaaagac
     6721 agaaagaaga aaaagaccca acggcgaaaa ctctcagttc tcggtaaaaa accgcttgtc
     6781 ggttctgcga tacgtaaaat ggcttctaac tggccacttt ttttgactgg taatgcgaca
     6841 tataaaagat cctcattcaa agtagtactt ttgcgcaaag cgctacctag gttcccacca
     6901 ttcaatacgg ctttgatttc gggacgggag tttcgctgtt cattcaatgt ttcattgttt
     6961 gtgtcaaata agatctttcc agtagcatcc attaaagtaa tccgttcgtt cgactgatgg
     7021 acgaattctt ccaaagcagt tttgttttgc gctgcttgga ggttttcgac atctaattgc
     7081 cggatcagca gttgtgcttt ttttgtcagg tagctttctt gctgttccac gatctgctgt
     7141 tgaaaataat gggaaatcaa ttgccagcca ccaaaaaaca aactaactaa aagaataaga
     7201 aagaagagac gttcataaat ttttttccat ttcatcgttt tggttcctga aacttatagc
     7261 caaatcctct aatagtcacc aagtactttg gatgttttgg atctcgctcg attttttctc
     7321 gcaaatgact aacgtgtaca tctacgattc ggctttgtcc tgcgaagtca aaattccaaa
     7381 tgcggtctag caatgtgtct cggtcgatca cacggtcttt tcgtttcatg aaataaacaa
     7441 gtaattcaaa ttctttcggt gttagttcaa tcagatggtc gtctacggtc acttgataat
     7501 tggtcaaatc agcagtgatt tgtccaatcg atagatattc aggctcagct tcatcttttg
     7561 attgcttacg aggttcaatc cgtctgaata ttgctttcat acgggctaat acttcgcgag
     7621 gactaaatgg tttggtcaaa tagtcgtcag cacctatttc taggccaatg atccgatcca
     7681 cttggtcatc tttagccgtc agcatcaaaa tcggtgtttc atttttttct tgacgcagcc
     7741 gctgagtgat ctccatgcca tccatcgttg gaagcatcac atctaaaatg atgaaatcaa
     7801 atgattgttc taatgctaat tctaatcctt cgcctccatc tgtggcactg gttaccttgt
     7861 agccttcttt ttccaaattg aacgtcaaga gcgttacaat agaaggttca tcatcaacaa
     7921 ctagtatttt tttcattcaa atctccttta aataggtttc attctgttta tattttagca
     7981 tagaatcagt acaaagggct attttacaaa agaaataaat ggtataatag gaaggcactt
     8041 tgctgaatag aaaggaagag cagtcaatga ttggaatcag tgcatgttta ggcggtctgg
     8101 cctgtcgcta tgatggacag aaaaaagaag taccagaatt gatggaacta gttgaaactg
     8161 gagaagctgt catggtctgt ccagaagtga tcggcggatt accaattcct agagatcccg
     8221 ctgaaatcat tggtggaaat ggattcgatg tgtggaaaga tcaagcaaaa gtatggacga
     8281 caagcggcga agatgtaacg gaagcgtaca aaaatggcgc gatcaaagcc tatcaaaaat
     8341 tgcaggaaaa acacattgat ctagtcatca tgaaggaaaa aagcccttca tgcggaaccc
     8401 attcgattta tgacggaagt ttttccggaa taaaaaaaaa tggtgccgga gtggctactg
     8461 cttattttat tcagcaaaaa atgactgtgc tctctgatga agaatggctg aaaactagag
     8521 gtgagcagaa tggaaatcga aaagaccaat cgaatgaacg cgctctttga attttattcg
     8581 accttattga cagaaaaaca aatgaactac atggaattat attatgcaga tgatttttca
     8641 ttaggtgaga tcgcggaaga atacgaagta agccgacaag cagtatatga caatatcaag
     8701 cggacaagca agatcttgga ggattatgaa aaaaaactcc atcttttttc taattatatt
     8761 gttcgagaac aggtattgga aaatatgact gcatatatta cagataagta cccggaagat
     8821 aaaaaactac ttgaatatgt acagcaaatt caagcaatcg aagaataaat aaaaggaatg
     8881 aagaaaatat ggcatttgaa agcttaactg aacgtctcca gcaggcgatg ggaaaaataa
     8941 gaaaaaaagg aaaagtatca gaagctgacg taaaagaaat gatgcgggaa atccgccttg
     9001 ccttactaga agccgatgtt aacttgcaag tagtcaaaga ttttactaag cgcgtacgcg
     9061 aacgtgcggt aggggcagaa gtgctggaaa gtttatcacc tgcacaacaa atcgtaaaaa
     9121 tcgttgatga agaattgacg atcacacttg gttctgaaac agcagagtta aataaatcac
     9181 caaaaatacc aactgttatc atgatggctg gtcttcaagg ggcaggtaaa acgaccttta
     9241 ctggtaaatt agccaattat ttaaagaaaa atgaaaatgc tcgtccgctc ctaatcgcag
     9301 gggacgtata tcgaccagct gcgatcgacc aattgaaagt attaggtcaa cagctagacg
     9361 ttcctgtatt tgatatggga actgaagtca gcccggtaga gatcgtccgt caagggatgg
     9421 agttagcaaa agaaaagaaa aacgactatg tattgatcga tacggcagga cgtctgcata
     9481 tcgatgaaac attgatggat gagttgaaac aaatcaaaga attgacgcaa ccaaatgaga
     9541 ttttgcttgt tgtcgatgcc atgactggtc aagatgccgt caacgtagct gatagcttca
     9601 accagcaatt aggtattaca ggggtcgtca tcactaaact tgatggggat actcgaggcg
     9661 gggcagcact atcgatccgc tcagtgacag gcgcaccaat caaattcatc ggttctggtg
     9721 aaaaattgac tgacttagaa gtattccacc cagatcgtat ggctagccgt atccttggta
     9781 tgggggatat gctgacattg attgaaaaag cgcagcaaga ctacgacgag aaaaaagcgg
     9841 aagaacttgc acagaaaatg cgggaaaatt cattcgattt caatgacttt attgaacagc
     9901 ttgatcaagt gatgggtatg ggcccactgg aagatctaat caaaatgatc ccaggaatga
     9961 atcaagtccc tggaatcgaa aacgtcaagg tagatccaaa agatgttgaa cgcaaaaaag
    10021 cgatggttta ttctatgacg ccagcagaaa gagcaaatcc tgatctgttg aacccaagcc
    10081 gtcgtcgccg aattgctgcc ggttctggaa acagtgttgt tgaagtgaat cgaatgatca
    10141 aacaattcaa ggaatcaaga aaaatgatgc agcaaatgtc caaaggggat atgaacatcc
    10201 caggtatgga ccaaatgttt ggttcaggcg tcaaaggaaa actaggcaaa atggcgatga
    10261 atcgaatgat caagaaaaat aagaagaaaa agaaaaaacg gaaataagat gaaaaaaaca
    10321 gcttcggtac agactgaaaa tgtctactgc cggagctttt ctatcagcaa tttttattct
    10381 ttactaatac aattactcta aacgaagagg tggcacagat gagatgtccg tggagtgaac
    10441 aaacagaaag tatgcggcaa tatcatgata ctgtctgggg tgttcctgaa tatgatgacc
    10501 aaaagctgtt ccgaaaattg atgcttgata ttaatcaagc cggattgagc tggcagacga
    10561 ttttaaataa aagtgaagca ttcgatgaag catatgatcg attcgatatc aaaaaagtcg
    10621 caaattatgg agaaacaaaa atcagtgaac taatggaaaa caaaggaatt attcgtaacc
    10681 gaagaaaaat cgaagcagcc gtccaaaacg ccaaagcagt cttgaaaatt caagaagaaa
    10741 tcggcagttt ttcagagtat ctttggtcgt tttctaatgg aaaaatcgtt gattcttctt
    10801 atcattcaca agaagaagtc ccaacaacaa gtcctctttc agatgaatta gcaaaagatc
    10861 taaagaaaag aggatttaaa tttatcggaa gcacaacaat ctatgctttt ttagaagctg
    10921 tcgggatcat caatgatcac cttgacagtt gtttccgcaa agaagaggtc gtacgtatcg
    10981 gcagaaaggg cgaataaagc atgcgaaaaa tcatatttta tggtgctata agtttagatg
    11041 gctatttggc aacgaaaacg gatgatcttc aatggctttt tgacactcca acaggtaagc
    11101 aaactactta tgaagctttt tatgaaacaa tcgatacgac catcatgggc agaaaaactt
    11161 atcaggaagc aaaaaaatat ttggagaccg aagcaattta tccagagaaa aagaatttcg
    11221 ttttttccac ccacgcacaa ttacaattgc cagatgctga ggtcgtaaac gaagatcctg
    11281 ttgcttttct aaaaaatcta caaatgacag aaggaaaaga tatctgggtt gtgggtggcg
    11341 gtcgattgat caaaccgcta ttggaacatc aaatgattga tgaatggtat atccagatta
    11401 cccctgtatt attaggggac gggattcctt tattccaaaa aggaacatat gaagcgcgtt
    11461 ttgaaatgct agacacgaca cgattcggtg aatttatcga attgcattat gtacgaaagt
    11521 agaagtaaat aaaataaaaa aggcaactat atgcagaaat gtcctgtaca tagttgcctt
    11581 ttttattacc gttatgcttt ttactctgtt aatttgttag aaaaatctag atacgcatct
    11641 aaaatcgttt ggaagaaact taatgttcct tcaacgatat taccattttc atcaattaga
    11701 ttcgcaacgt ttccgatata tgcttccggt tgctgcaagg ttggcacatt caaaaagaca
    11761 agtgattggc gtagatggtg gtttgcgcca aagccgccga tcccacctgg tgatgcagta
    11821 acgactaaac ctggtttatg atcccaaaca ctgtgtccat aaggtcttga gcctacatct
    11881 aaagcatttt tcaatactgc tggcacagaa cggttgtatt caggagtaac gaaaacgaca
    11941 ccgtctagtc ccctcacttc ctcacgaaaa cgagtccatt cagcaggagc ctgttctgga
    12001 gtttcgagat cctcattgta aaatggcaga tcatcgattt ttacaaatac actttcgtac
    12061 ccttcaggaa gcaatttcgc aaaagcttct gctacttttt tgttgtaaga atcttttctt
    12121 aaactaccaa taaaaaatcc aatttttttt gtcatacgat ttttcctcct tcaaactatc
    12181 tataataatc ttacaaaaga taagtaactg gagcaaataa aaacacttac tgacaaaagc
    12241 tacatagaag atagattact tatttttaac aaaaaagaag tctaaaatct tctattttct
    12301 aaaaaaaatc gaatttaatc aagctgtaaa gaaaaaaagc tttacagggt aaaaaaaaac
    12361 tgttacacta catcttgtga ataaaacaat ggaggtggaa ataaataatg gcagttaaaa
    12421 tccgtttaaa acgtatgggt tctaaaaaga gtccgtttta ccgtatcgtc gtagctgatt
    12481 cacgttctcc tcgtgatgga cgtttcatcg aaactgtagg tacttacaat cctttgaaag
    12541 accctgcaga agtagtttta aaagaagact tagttcttga ctggttatca aaaggtgcac
    12601 aaccttctga cactgtacgt aacatccttt caaaagaagg cgttatgcaa aaacaccacg
    12661 aagctaaatt ctcaaagaaa taaggtgacc ggatatgaaa gatttaagcg acttagtctt
    12721 aacaatcgtt cgtccgctag tcacttatcc tgatcaagtc aagttagacg tcgtagagtc
    12781 gaaagacttt tacgagtaca atctaacagt ggctcctgaa gatattggcc gtatcattgg
    12841 aaaacaaggg cgtgttgcta aagcaatccg cacaatcgtt tacggtgttc ggatcaatgc
    12901 accgaaaaaa gtgcgtttga atatcatcga tgataaggaa aagtaaataa acgtgctgta
    12961 ctcgaatttt cgagtgcagc ttttttgttt tttttattaa ttgaagaaat gattatttgg
    13021 caaaacaagc aagaaagcgt atccttatag gaggagggag tgaacagcca atgagaaaga
    13081 aaatgttatt tattccattt gctctattgt taagcgcttg tagttcggtt actcaagaat
    13141 cagacaatac acatctatca agcagtatat ctgaaactgt taccagtcca gaaaaaagta
    13201 gtaccaaaac ggcttccacc actgaacaaa caatggctac ctcaaataat gagaaaaaga
    13261 ctgctttaga ccagttgaaa gaacaacagc caaatgttcc catgcctcta gatgtaccag
    13321 tctcttcagg ctatctcaat atcgcagcga cacatacaaa gcaaggttat tctattttat
    13381 attacagaac agatcgtccc ctcgggttga atgctgacga attgaatcaa gaaacgccta
    13441 ttgcaaccta tctatatcaa tatgggtttg cttctagtca agaaacaatc caagttttgc
    13501 agccatttga gatcgacaca aatggtcagc aagtggactt aggttctcga atcacagggt
    13561 atcagcaagg tgcagcaggt tcaagttttc ttgaatggca agaaggaaac tggcgtattc
    13621 gaatccgtgg gaataatata gaaggccaag atcccttact attagctaaa gaaatcgtag
    13681 cttatttgga agaaaacagt ctaccagcac ctgaacaatt tggtaaaatt actgttgata
    13741 tgggtgatac gacgaatcga gccgtagaag taagctggca agaaccgaaa aatgcctata
    13801 cgatcactca tcaagatcct atgtctgcgt tgaagatggc agtttctatg aagaggttgt
    13861 gaaatcagcg cgctgatctg agcccatcat ttaactccaa gtcacaggaa gtaatcaaaa
    13921 atagcacgcc gcatttttgc ataattttgg cttaatacct taaaagaaaa ctagaattgc
    13981 ctcttcacac atgctaaaat aaaacagaca aaaaagaaat ggggatataa gttgactgaa
    14041 tacttgaatg taggaaaaat cgtaaacaca caaggtatca aaggcgaagt ccgtgttatc
    14101 tcaacaacgg atttcccaga agaacgttac aaaaaaggaa cggtgctaac actattccaa
    14161 gacggaaaag cacctgtgga attaacagtc aaaagtcatc gcaaacataa aaattttgac
    14221 ttattgagtt ttgaaggaca tccttctatc aatgatgtgg aaaaatatcg ggatggcata
    14281 ttaaaagtgt caaaagacaa ctctgtagat ctagctgaaa atgaattcta ttaccatgag
    14341 atcatcggtc tgcatgtatt agaagatgga actgagttag gtaaagtaaa agaaatcttg
    14401 tcaccaggtg ccaatgatgt ttgggtcgtt caacgtccga aaaaagctga tatattgctt
    14461 ccatatatcg cttctgtcgt caaagaagtc gatttagaag ctggagtggt ccgtgtagaa
    14521 attccagaag gactgattga cgatgaagat tgatgtattg actttatttc caagaatgtt
    14581 tgaaggtcct atgggggaat ccatcatcgg taaggctgta gataaaggat tattagatat
    14641 caatatctct aacttccgcg attattcaga taacaaacat cagacagtag atgattatcc
    14701 atacggcggt ggagcaggta tgctgctcaa agtccagccc atctatgata acatccaagc
    14761 cattgaaaaa gctgcaccgg atgtcaaaaa aagagtaatt cttcttgacc cagctggtaa
    14821 acgattcgat cagaaaatgg cagaagaatt ttcacaagaa gaacacttag tattcatttg
    14881 tggtcattat gagggatatg atgaacgtat ccgcacatta gtgacagatg aagtttcctt
    14941 aggagattat gtccttactg ggggagaatt aggtgcgatg gtcatgatcg atgcaacagt
    15001 tcggcttttg ccagatgtgt tagggaatca gacttcagct caaacggatt cttattcaac
    15061 cggtttattg gaacatcccc aatatactcg cccggcagaa tataaaggga tgaaagtacc
    15121 agaagtattg acaaatggaa atcataaatt gattgaagaa tggcaattaa aagaatcact
    15181 aagaaggacc tatcaacgtc ggcctgatct tttggaaaca cttgaatgga cacctcaaat
    15241 gacgaaattt ttagaagaaa tcaagaaaga agaacaagaa aaaacagcag atgaagccaa
    15301 gtgacttttt ctaaaatctt ctttacagta ggttgataat atgttagtat tttatggtga
    15361 gtgcaaaagc acttaactat tacgatattc cgctgtgaga aaagctcata agaatatttg
    15421 gaataaggag aagaaaacca tgaatccatt aatcgaagaa ttaacaaaag aacaattacg
    15481 ttctgacatc ccagcgttcc gccctggtga cactgtgcgt gttcacgcaa aagttgttga
    15541 aggtactcgt gaacgtatcc agttatttga aggtgttgtt atcaaacgcc gcggtgctgg
    15601 aatcagcgaa acttatacag tacgtaaagt ttctaacgga gtgggcgttg aacgtacatt
    15661 cccattgcat acaccacgtg ttgcaaaaat cgaagtagtt cgctacggta aagtacgtcg
    15721 tgcaaaattg tactacctac gtgcattaca cggaaaagct gctcgtatca aagaaatccg
    15781 tcgttaatca tcgttatcag acagagaaag aacccttggt attcaagggt tcttttttta
    15841 ttatctgttt ttttatatta acgatttcta tttggtacat ttttgattaa taaaaagggg
    15901 caaaaaaggg gcaacaagtt acagtttttc tatatcttct ttcaattttt cttttactcc
    15961 ttttgtcaca tgcaaataaa ttttttcagt gatatcacta ttttcgtgac caacgcgatc
    16021 ctgaatggca tacagaggag ttcctagttc cgctaatttt gaaatatggg tatgtctaaa
    16081 aatatgtgaa ctaagttttt tatcaatctt catgtcagct ttatgacttc tcaaataggt
    16141 gttaattgct gttagttgga aaggagttcc tttcgttgtt tgaaatagaa attgaccatt
    16201 tggatttaat tctagaagct cattgtaaat agctatcgct ttttttggta aatcaatttc
    16261 tcgcattccg gcagctgttt ttgtagaatc agattttttc atatcagcta ttgaacgttc
    16321 tctatacatc atcgtcccat ttataactac tgatgcatta ttattggtaa tgtgtacatc
    16381 atctttactt aatgcgattg cttcaccaga cctcatccca gtcaaatata gccactggaa
    16441 aaggagagaa tatcttttat tgtgtgaagt tgtgaaatct actagtctat tatattcatc
    16501 atcttctaaa aatttatctt taatttttat cgtttttgac tctcttttat aatcaataac
    16561 tacttcatcg ataggatttt tttctacata accttttttc atagcatatg aaaaaagaag
    16621 attcaatttg gatttaatta cactgacgta cttatttgac aagtcatctt tatatatcat
    16681 gtcttcaaaa atattattca aatctattgt attaatacca gaaacgatgt aagtctcagg
    16741 tatcttcttc tttatagtgt ttaatatatt attcgtagga taatatgtag attccttaac
    16801 ttgtcttttg tatataacta accattcttc aacaagctca tgaaatgtta gatcaggctt
    16861 atgaagtgtt ttttcgttta attttatatc aattttttta tttaactcta gcaatgccac
    16921 tttctgagtt tctcgagatt tatttttata agtaatgctc actttttttc tttttctagt
    16981 ttttggatca atatatcgct cattatattt atatactttt tttccgtttt tatcagtttt
    17041 agtttcgatc cacattgttt tcatctccta tttgttgcta taataggaat agataagtaa
    17101 gccgattata gcaggtttat ttttcatcac gtccacaaac tttggcgagg gaggggcgtg
    17161 tttttgttag ttattgtggt aggttatcta aagcgtattg tgcttgctca tttgtaaaac
    17221 cttcaaatat aagttgatcg tacaattgcg aatctgacat tgatgcccaa tcatcgtaac
    17281 tttccgcttt agccaaagct tgttcgttgt aatcgacatc aacattagcc agcgcataat
    17341 cgattgcatc acttggatat tcttcaaact ctagttgttc acgcaaacct tgttcagaca
    17401 taccagccca acctagatag ctttcagctt tgctcaatgc atttctgtat tcacgaggaa
    17461 cactttcttc ttgagtaact tcttctatag tagtttcagt agtttcagtt tcttcttcaa
    17521 cgactgtaga ttctgctacc gattctgcag aagacacttc tgtcacgctt tctttagttg
    17581 tttcagcgac ggtattttta gcatcatcac tgcctccttg cgaacctata gcaataaaca
    17641 ccacaacaac cgctaacaac caaaaccata cccgtttata aaacggtttc ttaactttat
    17701 acattttccc atcttgaccc ataacttttt ttgccattta aatatccctc gcttcttgtt
    17761 ataatatatt tgtgatctca gaaatgaggt atgagtccgt gttgcagcac gggctttttt
    17821 ctttataact tttttagaga ttataggcaa aatagtaggg cataaaaatg tattattgaa
    17881 ttccgtattt agaaaatcct aattgaactt caccggaagt cttttgctgt gttgtacgca
    17941 atgcttcttc ttcagacatt ccattctgta ctttccatgc aacaggcgac atcccgtatt
    18001 tgttaacaaa atcagtaagt gataaagtgt cagcgtcttg ctgagcgctt gtttgttggt
    18061 cttctggatt ctgttgagat gctgcttgtt gttctttctg atcttgactg ataatattgc
    18121 cagcatcatc tgtagtcaat ccattttcat aaagggccac gccgaaagct tcccactctt
    18181 tgttggacca atttgcacga tcagctggag ttgactgtaa agtgcgttgt ttcatctgtt
    18241 catatgtttc ttcttgaggt gcggtttgga ttgtactctg actggagctt ataactgttg
    18301 ggctaggttc cgccgtagct tggttggagc ttgtaactgt tgaactagaa tctgttttag
    18361 atgtagattt gctagtagat gaactggttt cagttgtttc ttttgtttta cttatttttg
    18421 tttcttggtt agaagtggta tctgttgatt cagctttttt attatttgaa caagctgaaa
    18481 gtagcagagc agtacttaac aacaacataa cgctaacttt tttcatttta taattcctct
    18541 ttctcgttgt aatatgtgtg ctaacacggg cttttttata taagaaaacg ataagcgctt
    18601 tctggaagcc cgtaaagatt ctttaattcc tcgatttttt taggatattg atcattatct
    18661 tctttataaa gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg
    18721 cttctagatg ttcttgttga tacataatag ctggataagc cacgatgaaa aatagcgtga
    18781 cctaattcgt gagcgcaaat gtagaatctt tcctcagagt ctcgcagttc atcatttaaa
    18841 aagattatcg cacgatctct aatttcttga aactgcccct tgggattctc gataaaagga
    18901 acgtattgaa ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat
    18961 ttccgcttca actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt
    19021 gcctttttct ttgtcttctt tcacaatatc ccagaaagtc gctatcagga tatcttttac
    19081 gcgctgtatt tgttcgggtg tcaatgtttc cccaccataa gacatattaa catttgagtc
    19141 tagtagttta tcaagttcaa ccacttcctc ttttgtagcc cacttgggaa cattattatt
    19201 tcccaataaa taatcagttg tgacaccaaa ataatcagca actttcttta agttctcaga
    19261 ttttggcgag gctttatccc atcttcttat ttgtccatta gagatgccca cctgtctttc
    19321 tacttctgct atagtcacat gcttttcgtc tgctaattct ttaatcttag taactaaact
    19381 cattattatc aacctttcaa agctgaaaag aaaataaata gcttaaaagt tatatttttg
    19441 gttgacaatt agcttttaag ctagtatatt taattcgtaa gctaaattgt tagctaaata
    19501 agagcaacaa aaaactctac taatttaaaa cattctctcg gtcgccaaac ttagaaatgt
    19561 tattttagag gctttttata agtcttattt aactatgtat tcataatagc ttaaaagcta
    19621 ataggtgtca acgatttagc taatttttta gcttacaaat tatttgttta gaaaggagct
    19681 atttttatgt ctgagaattt agacttaaaa attcgagcgg agatgagaaa aagaagaatg
    19741 actttcaaag aactagctgc gcttatcggt atttcaggag cttatttatc agatattcta
    19801 aacggcaatc gtgatggaaa gaaagcacaa cagcatatcg aaacagtgaa aaaaatattg
    19861 gacatccgat agggggggta ggcgatgaca aaactaaaaa aacaagattt tgtaaaaaaa
    19921 tataattatt ctccatctac ttatcaacgt cgaatgtcgg aactaaaaaa tacagcaatt
    19981 ttctcagcgg cgtatgaacg ggtcacagga caagaagttt ggatcaatac agaattatac
    20041 gataaatttt tgtctttcaa atcctataac aggttacgca caagaaaggt aacacctaaa
    20101 gaatttatcg agaagcattt agttgattta taaaaaacag aacattttga gaggtgaagg
    20161 ttaatgggta aattcaacag agcattagta ttcagcgcac cgctaatcgt ctatgcttta
    20221 gggctttggg gaagtagaca agcgttgata ggaacgattg tgtacatggt ttggattttt
    20281 atagggctgg atgaagctga gtacaaaaca aaaaagccag tcgggaggga ctgactaatg
    20341 agtaaggacg atattgtatt tctgttaatt atgttttgtg ggattggatc aataataatt
    20401 aatttgtatt tgattattaa agaaaaattt ttcaattaaa atgagtcttt tagctttaac
    20461 ttaaattttt tttgataatt agctgttctt atgaccatta caagtttttc accaggtaat
    20521 atatctttca tatctaaaac ttctgaaaaa gcgactgagt agccaggaga gattgcttgt
    20581 gccaacggta taggagataa aaggctactt ttatgaatgc ttcccatatc aaaaccattg
    20641 gcgtgataat gtgaacaatt aattttctta ccgttgtaaa gatacgtaca ttcgataatt
    20701 gagtttggca tttttgatgg gtttgcaatt acgccagtaa gaatcattcg attacttcta
    20761 tcatgcttaa ctaaaaaata ggagagcgta atttttcttt tatctttaaa taagttatag
    20821 agggataaaa aaaatcctga aatagaaatg attaaagtaa tagcgacttg gtaatcgctc
    20881 aaaattttta tcaagtaatt caccaccttt ttactcaaat tatatcaaaa aggagagaag
    20941 aaataatgca agaattagta attttgaaaa ataaaaaaag acacgaacgc cggcaagcat
    21001 gatcgtgtca gggaaataaa ctatctttct atattttacc acagaaagga atgaaccgta
    21061 aatgctaatt gcaacggata cactagaccg catcttttta aaagacgaat acaaactgcg
    21121 caatatagat gcgtcaggaa ttttagtatt tgatctttat gacaatggaa aaattggtat
    21181 ctatcaagca agtgatattg aagaaacaaa ccttgctttc gaacaaattg atgattctgt
    21241 ggaattggat ttagatgagg caatcctagc ttttgaacaa attgcaaaat tattaaagga
    21301 ggcacaaaag aatggcaact ctttaccaac tcagcgagtc atatatcaaa gtcctggaac
    21361 tggcagaaca attggatgaa gaaattcttc gcgatactct cgattcgatt aatgaagcga
    21421 tcgagtataa ggcagaaaac ttagcaaaaa tagttaaaga agtagaaggg aaagctgagt
    21481 taatcgatag tgaaattaaa cgtctgcagg aacgtaagac atcgcttttg aacaatgcta
    21541 agagtatcaa gcactattta caagaggaaa tggaaaagac tggtaaaacg aagattaaag
    21601 gtgaattatt caacattgga attcaaaata atccagtatc ggtcaatgta atcaatgaga
    21661 acttaattcc aaaaggattc tttacccctg tgcctcccaa attggataaa aagcaattga
    21721 aggaggaact gaagcacgga gatattcctg gtgctgaact cgtccaaaca aaaggtttga
    21781 ggataagatg atgaaggagg agaaataatg gaacacgaaa acgtaaaaga cgctttgaaa
    21841 gctgctatcg aaatcgcgga agctaaagga ataaaggttg acggcaaacc agcaactgtc
    21901 catgacattc aaaatttgac gaaagaacat ctttatttta tcgccgactt gcttggtttg
    21961 tcggaattgt atttagacaa ataaccccac acggaaggag tggctagggt ggttgtatta
    22021 caagtaatag tctgcgtaac attcatttgg tcgtgcatac cggtttgtat atatatcatg
    22081 aagaagcttc gccctgagtc ttacaaatta tacgcaattt acagcataac agtagctctt
    22141 gcatttacat tgtttgcaat ttctaggctt ccgttttaat cgtgggaatg aatttttaat
    22201 gaactaaatt atctataaag caaaaaaagg agaaacaaca ttgaaaaaca aactagcaaa
    22261 aacaacagca atcatcggac tagcactagg tagcggcgtt atcggctacg cagcaagcaa
    22321 tgcgtttcaa gatttggaca cgatcaaagc aaatttcaac acagtcctac aatacggtca
    22381 aacgaaatcg caacgtgtgt cagaacttga atcacagcta tccaacaaca ctcgcacaca
    22441 ggagcagctg aaagccgaaa ttgagcaaat caaatcggac aagcagaagg aaattgaagc
    22501 caagcaacga gaagtcgaac aaaagcaaca ggagatcgct acaaagcaac aggaagccga
    22561 tagcttgcgc caacaactat ccacagtgca aaacgacaag gagcagttag aacagcgcgt
    22621 gagcgagtta cggcagtata cggatcaaaa ggtaggggag ttggggaaat gaaagagttg
    22681 actatttatg acccaaatat ttcatgggct aaacacacaa ttaaagtttc ttttatgatt
    22741 tggggttaca aaggatatgt tacttacaaa gtgggcggta acactaaggg tttatcatta
    22801 atagctattg attcagatga tctatatgat gctaatttcg aggataaccc agttaatttc
    22861 cgtgaccttg atgaagattg gttctctatg gaacttacta acgataaagg cgacagtaca
    22921 cttgtagaag atgaattcga cagactaggt gattatattg tcggtgttga aattattgcg
    22981 cacgaaccag agtaggagag tgaatacaaa atgattaaaa gaatcactat atcagttgaa
    23041 gaagatagtg gatcaacaat gcagcatacc gacaatcgaa aaactttttt aaccaataga
    23101 aaggagaatt ttgatgcaga ttaagaaggc gacggatatc gaacaaacca agggaacata
    23161 tttgatctac ggtgctccag gaaaaggtaa aacttcaacg gctaaatatt ttccagggaa
    23221 aacactgatc cttgatattg atcggacttc aaaagtcctt agaggtgaga aaaatgtcga
    23281 tatcgtatat atcgacaacg aagatacctg gaacgactgg ggcaacacgt tggctgattt
    23341 aactaccaat tatgtaggtg tttatgacaa cgtggttgtc gataatgtca gcgaattgga
    23401 acggtgtatt ctgtccagtt taggcgcaga aggaaaaaac aacggcgttc cttcgcaagg
    23461 cgattatcaa tatatgcaat ttagaatggt gaacagccta cgctatatga aaaatttaga
    23521 cagcaacctt atctggaccg catgggaaga aatcgatttg tggacggatt cggatggatc
    23581 ttcttacaac atcgctttgc cgcagatcaa tcgaaagatt cgaaacaata ttttgggatt
    23641 atgtgacgtt gttggtcgat tgatggtaaa ggaagacgat gaacgcggat ttattttaca
    23701 agccacagat tcaacctatg ccaaaaatca attggataat agatctggct gtaagcaaag
    23761 tgagctgatt ttagatggaa ttgtacgact accagaatga tttagtcgat cgtgcaagac
    23821 aagcctatgt tgatggttac aaggcaccat gtatcgtatc accgtgcggt tctggaaaat
    23881 cagtcatggt tgcagagatc gctaaacgaa ccacacgaaa aggcaacaga gtgttgttct
    23941 tggtccatcg tcgtgaattg attgatcaga tcaaagatac gtttcaaaag atgggcgtag
    24001 ataatcagct tgtaaatttc ggcatggttc aaacagtcgt acgacatctt aaaaccatca
    24061 agaaaccaca actgatcatc actgacgaaa accatcacgg gttagcagcg tcctacagaa
    24121 aaatctatga gtattattcg gacgttccgc gattaggttt taccgcgaca cccatccggt
    24181 taaacggtag tgggttgggg gatgtgaacg acttgctaat tgaaggagta tcggctaaat
    24241 ggttgattga gaatcataga ttagctcctt atgaatatta tgcacctaaa ttgatcgata
    24301 cagccgaatt aaagaaagct tcgactggag attttacaaa gaaatcaatg gataaggcag
    24361 tgaagaatac tatttatggc gacgtgttga aacattataa aacgttagca gaaggtgaac
    24421 aggcaatagc atactgtcac agtatcgatg caagtaaaca cactgctgaa atattcaacg
    24481 atgctggtta taaagcaaca catatcgatg caaaaactcc aaaagatgaa cgtgcagata
    24541 ttatcgaagc gttcagaaat catgaaatta aaatcctatg caatgttgat ttgatcggag
    24601 aagggtttga tgttcctgat tgttcgactg taattatgtt gagacctact caatcgttat
    24661 cattgtacat tcagcaatcg atgaggggga tgcgttaccg accagataaa acgtcaatca
    24721 tcattgacca tgtgggtaat gtgaaccgtt ttggattgcc tgatatggat cgtgaatgga
    24781 gtttagatac aaaaaagaaa acaaaaactg atagtgattt atctatcgtt caatgcgcgt
    24841 tttgttttgg tgcttacgaa cggccaaaag gcgataatat ttgtccgtat tgcggagagt
    24901 tgcagccgat tgaagaacga aagtcagagt tagaaatcga tgaatcggct gaattaatga
    24961 aagtcggaga aacgaaaatt actttgaatt ttgagaacaa taaatattac aacatgacgg
    25021 aagatgaagc agaaagtata gaggacttat atgccatagc aagagctaaa ggatttaaac
    25081 ctggttgggc ttatatggct gctaagagga aggggtggtt gtaggaatgg gcggtatttt
    25141 tggtaaaaaa gcagaagatg ttcaaggttc aataataaat ggcgtaacaa taataggtga
    25201 tactggaaaa agagatagtt caggcaatca aattgtaatc gctagaaata aaaataacga
    25261 agtttttgaa tcttctctat ctaatataaa aagaggtgtt gttaacggga caggcgaaga
    25321 gagtggctac gttaatttaa aaaaagctaa tgaaaaatac catgttgaag gaacttacgt
    25381 taaggctttg tctgtcggtg gaacaaaagg cgttaattgg gataaacgaa gaaataaatg
    25441 gaaagcacga ttatatttca aaggagaata tgtcttacaa aaggatttca acaccaaaga
    25501 agaagcaatc caagcacgac ttgcagcaga agaaaaatat ttcaaaccta tcttagaaaa
    25561 atatgaaaat aaaaaggaga atgattaaca tgacaggatt taacttagac ttttcaaaag
    25621 cgcaacaagg gaacgaaatc aaagacggta cgtacgaagt ggtcgtaaac aaagctgtag
    25681 aaaatgcaac taaatcaggc gcagaattta tcgatatcga tttgatcgtt cgtaacgatg
    25741 tggatcaacc gttccaaaat aaacacattt tcgcaaaaat ctggaaagcc aaagctaccg
    25801 gcaaatataa cgaaggtatg atcatggcaa ttgcacaagc gctacagttg gaagacggaa
    25861 aatcatataa cgggtttgat gaattgctgg cggactttgt tttgaaaacc gcctcagtac
    25921 gcgtgaaaac tgaagagtca aatggatata aaaacgtcaa tgtgaagtct tgggataaaa
    25981 cgaacacacg gggcgtaatg aatcaccaat tcaaaaatgg tgatgaacca tccttcggac
    26041 cggaacgttc aagagcgaca acggttagaa atgacaatct tccattttaa ttgaggtgta
    26101 atcaatgtat gaaaaaattc caaaagagct taaaaatctc aaacaatggt gtgtttataa
    26161 gctagtttgg gatgaaaagc ggcagaagca cacgaagatc ccttataacg ccaacaacgg
    26221 gcataaagcc aaatccaatg acgagagtac ctggtctgat tttcagaccg ctctcgctgc
    26281 tattgatcac tacggaatga gtggactggg gttctttttc aaaccacctt atttcggcat
    26341 tgatatcgat aatgctgaag gtgaagttga gcgatttaaa tcaggtgata tagaagaaaa
    26401 tattatctat gaatttatcg aaagcatgag atcctatgct gagtattctc aatcaggtac
    26461 tggcatccac attattgctc gcggagaatt gcccggcgga agacgcagga aaggtgacgt
    26521 agaaatgtac cagaacggcc gattcttcgt tatgactgga aatgccgcat caaaatattt
    26581 agaaataact gagccgaatc caaaggatat caaacgccta tatgatcgct acgttggaga
    26641 tgaaaaaatc atccaattca aagaagaaaa tccgctgatg aataccgttg atttaccggt
    26701 tgaagaaatt atcaaacgtg ctgaatcgtc ttcccaaggg gcaagattca aagtctttat
    26761 gaacggcggt tgggaagctt tttattcctc gcaatccgaa gcggatatgg cgttcgccaa
    26821 cgacttggct ttttggaccg gtagagactt tgaaaaaatg gatgagattt tcagagcgtc
    26881 tgcaatgatc cgtagcaaat atgaccaaaa aaggggatcg gcaacttacg gagaatctct
    26941 actaaataag gcaattgctg atacaaattc cgtctacaac cccaaacgga aatctgattt
    27001 taagattttt atcaaagacc aggaacagcc aaaagaggaa aaatactatt cgtacgacga
    27061 caccggcaac gcggatcggt ttactgatat ctacggaacg ttagtcaaat attcgtacat
    27121 tgataaatct tggtattact acgatgggaa agtttggcta caggacaata ccggcgaagt
    27181 tcggaaaatg atcgatacga cagtagacat catgggaaaa gaaccgttaa cgatccctga
    27241 aggcgcagac gatgaaacca aagaagcttt gatgaaagcg aaagaaaaac acgtcaagcg
    27301 ttctcgcagc aatgccggaa aaaacgctat gatggacgaa ttgaaacatc gactatccgt
    27361 attgccggaa gagttcgaca aagataaaac gctgttcaac acacaaagcg gctatctgtc
    27421 tttgcatgac ggtcttctac acgagcatga gatagacaaa atgtttacac gtgtttcaaa
    27481 tgtcgagtac acagaatcgg tcgattgtcc tatgtgggaa gaatttatta atcagatttt
    27541 tgacaatgat caagaattaa tcagatatat ccagaaatgt gtcggatatt ccctgaccgg
    27601 atctaccaga gagcaatgta tgttcatcct ctacggtcat gggtcaaatg gaaagtcggt
    27661 atttttggaa attatttctg aattgatggg aaactatgcg atgactatgc aggcacaaac
    27721 gatcatggtc aagcaaagcc aatcatcagc aaacagtgat attgcacgat tgaaaggtgc
    27781 gcggttggtc acttcgtcag aaccgaatga aggcgtccga ctggacgaag gtctggtcaa
    27841 acaattgact ggtggtgata aagtcacggc tcggcacctt tatggaaaag aattcgagtt
    27901 cgaacctgaa ttcaagttgt ggctggcaac gaaccacaag cccatcatcc gtgggaccga
    27961 tgacggcatc tggcgacggt taaatttaat tccattcact gtccaaatcc cggatcacaa
    28021 aaaggataaa aatctgaaat ttaaattgca gacggagttg caagggatcc ttaaatgggc
    28081 aatcgatggt tgcctgttgt ggcagagaga gggcttggag aaaccaaagt cggttgttgc
    28141 agcgagtcag gattatcgaa acgaaatgga tcaaataggt acatttatcg aaacgtgttg
    28201 cgaaacaggt ccaggactaa aaatatcagg cggagaatta tacaaagttt atcgtgaatg
    28261 ggcatcagat aatggcgagc atactttcac taacacgaag tttggcagag agatttcaaa
    28321 gaaatatagc aaagaaaaag ctggtggatt tatggtttat aaaggaatca cattaaaacc
    28381 aagaaaatac gataacgtta gagaactttt taaataaatc tggagggttc tggagggttg
    28441 agaacgaaac cctccaaccc ttagaaacgt tgatatatca gtatttatat tatatttttc
    28501 tttctttctg gagggtttta aaaaaataat aaagtattaa agtaaaaaat aaaaagtaaa
    28561 ttatataaaa aagaataggt ttttgggtct gaccctccaa actctccacc aaaatcttcc
    28621 taaatgcttt gataccaacg attaaacagt ctggagggtt acattgaaat cagaacatca
    28681 aatacaagac gagatcagaa ttgccttatc aaaacatggt tgcacgattt ttcgatccaa
    28741 tgcgggacaa gtgacaacga tcgaagggcg gcaattttac gggatgccaa aagggttccc
    28801 agatctatgt ggacatcgta attctgatgg gaaagcaatt tatatagaag taaaaaatga
    28861 acgcggtaag ttgcgtgaag atcaaaaaag gtttgctgaa tttttaaacg gtcaacctgt
    28921 actatacggt gttgcacgat cagtggaaga agcattgaaa attgtggagg aatcatcatg
    28981 acaacagaag aagtgattca aatgcgtatt cgaagccttc agcgtgagat tgacgatctg
    29041 gaacggacaa aggcagtgat ggtcaatgaa acggcgagga aggcaatcga tttgcacata
    29101 gagaatttaa gaagggaaat ccatcgattg gaggaatgag cgtggataag aaagcagcaa
    29161 tgaaacgaat catcgaactg acacattctg agaattggca agaagacaaa gaaatagttg
    29221 cagaagtcca aaagctcggt aaatcaatgt ggagtgaaaa aaccaaacgg agaacaccga
    29281 gaaaaattgc aatctggcat ggtgaccgaa ttctagtaac aggtactgct gaacagttat
    29341 ctgaaattac tggattaagc aaaaacatta tctgggatag agctaaacgc gagaacgttg
    29401 attcgaaagg tcgtcaattt aaacattggg agaagaaata atggaggaac taatcacaaa
    29461 agtagagcag tgggcaaaag ataagggatt ggatcaagct gattccagca agcaaatgtt
    29521 aaaaacgatc gaagagattg gggaagttgc cgcttctcta gctagaaaag atgaatatgg
    29581 tttaagagac ggaattggag acgtagtagt aaccttgatt attttagcta tgcaaaatga
    29641 tatggatttg tacgagtgtc tgaaccaagc atacaacgaa atcaaaggac gcacaggaaa
    29701 aatggtagat ggtgtattcg tgaagtcgag tgatttggag gacagcgatg aataaaaaag
    29761 tattaattga taaacaagca ttgattgatg aattaatgaa aatacctggt gtgggatcta
    29821 atagtgacgc tttagaaacg attaaacgtt tcccatcgta tgaaccgcag aagccagttg
    29881 tgcctaagtt tgtggcggaa ctactcgact attatcgtca gtcaacagat gttgatttat
    29941 tagctttatt gataactttc catgattggt attatcgcaa aactaaagac ggtgagcatg
    30001 aagaagcaat tgattggcta gttgatcatc ctgaaattta tatgcgtgca tggctgtttg
    30061 gctacgagct cgagaaagag ccgttgtatt gcgtaataat caatggtaga tatcttgtca
    30121 aagtattcag caatacaaac gttgtcatat tagttccagc agatgaattt tcacagtacg
    30181 ttactcaaaa atttcaatta actgaaaaac aaatcaaagc aattgatgaa agatactggc
    30241 cgtttgctgt gccagtggat gaggtggtag aaggatgagc gacccataca ttactaaatt
    30301 agaaaagttg tccgaagatt ttgagaagaa taaaaataaa ctaactaaag ataaacttat
    30361 agaactggtt aggttggtta tcagaggagc tattacaaga gaaaaggcgc agcaaaacat
    30421 agaaattttt gctcgtagtg tccgagatgg attagagaag aaagttaaac aaaatgaaca
    30481 acagacaccg cagaataaca aaactaagaa aacgggaact gaatgtacta aagacaaagt
    30541 ttgaaaaaga atatggaatt tcagcagaag aaacatataa agtggcaagt cagtgtgttg
    30601 ctgatgcgag cggcgctatt cgtaagtttg gaatttcgat attaagtgat gatcgtaaat
    30661 gggaggaaaa agaatgaaac taaaagacgg attttactcc agcagtcatg gtatcggcgg
    30721 tttaatgcta gatatgccga caaagaaccc taaaacacgt gagaaaccaa aattcaaagt
    30781 cggtgacatg gttcgctgtg aagcagaagg attcatctat ccattttgtg gatatgtaga
    30841 acatctctat aatcactcag caatcattcg tattgaaaac acgatggaat gtgacaagtg
    30901 gttagcgaaa agcaaagaga atttagcagt agcgagattg gtggatatgg aggttatata
    30961 aacagcgaaa aaatgtactt ctaagagaaa aatttatttt ttataaaaat aggaaagtat
    31021 cattatgaaa aagaccacag agataaataa acctaccaca taaaaaatga atatttcttg
    31081 cgattcaata taggtatatt taacaatttg tacaataaaa aatactatag gtaaaaaaga
    31141 taacatgagg agcttatttg ttctagagtg tttgacaaag taacaaaatg cgacgatacc
    31201 aatacaaacg ggaatccaaa ataacagcat gctccacata gtcactcaac catctcctat
    31261 acgctttatt atcaatagat aatatcattt ttcgtaataa agataaaaaa ataagtaagg
    31321 cttctcatta aaatataaaa aagacagccg accactgact gcctatataa gagtattgaa
    31381 ataaaaagct gctgatataa taaattccac aagtttatta tatcacataa aggagcggtt
    31441 tgacttgatg caattgttac gagaggtaga tttcaaacag acaagatgta atgcgagaga
    31501 tgtgctgaag aactttcggc gtttggagcg gatggcaggt cgctctttga tagatattaa
    31561 gtcgccgatt attacggata tgccgaaggc gccgaagcac ggcaataagg cagaagacgc
    31621 aatcattcag atgatggata tagaagcgga gagagacgcg attctagcag ccttgatggc
    31681 tcttagtctg attagtcgtc agatactcta ctatagcttt tgtgtgccag atagcttctc
    31741 aaactacaga attagccgtg aagtgggtta ttcagaaaga agtatacaac ggatgaagtc
    31801 ggaagctcta atagagtttg cagaagcata taaacacgga agaataattg cttataaata
    31861 atttggcggc tttttggcgg aatgatggcg gtttttagct atttaccagt gatattatgg
    31921 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
    31981 attattgttt cacaattaag cttcgataga cagcaacgga aatattaaga ataaggatgt
    32041 gaattttaac tccttctaaa ttgttcttat tatctatcat ccgttgctgt ctattgtttt
    32101 tttaattatt cacacgataa ctaaaggtga gcgaagagaa atgattccat taataatttc
    32161 aatttttgcg ctttgtctta atgtctatat gattggattt aaaaatgggc aaaataaaag
    32221 atagtagtaa ctaagaaaga aatttttata ttgtcactgt ggcggaaagg gtagacgcta
    32281 agcatgtgtg ctaggtcaat gcttcggcaa ctatgcaatg ttcgattcat tgccagtgac
    32341 tttggcaacc gaggcatcgg cggtttaaaa atataggggt gcgcaatttc gtacgcgttt
    32401 tgtgcatcgt gtaggttgct attacatatt agatcactcg ttgagtggtc tttttatttt
    32461 acataaagga ggtaacaacg atgtatagac cacaatactt agaacagaag tatgaagtaa
    32521 tcactgtgca aaacggtaac ggtgagatag tacgaaagta tagaagacca ataaagagcg
    32581 atacatataa acgaaaggaa aacaatgaag ttatttcttt tcgcagaagg agaaaagcca
    32641 aatgagaaac tactggtatg tatcgctaac taatgaatat cctcgaacca ttgatgattg
    32701 ttcagtgcgt gttgtgcgtt ctgtacaaat caaagggaag tactctattg tcgaaatgct
    32761 aagagaagct acaccaaacg aagtggataa atgcaagctg atatattgcg gtcatggcta
    32821 ttggaaagac gagtatatcc aacaaaacat tgagaggtgg atagatagat gagttacctc
    32881 gaacatttga aacgttgcta catgcattct aagaataaac ttcctgacag ctacactgta
    32941 gatgatgttg ctatacatgt attgaagact gagagccata gcagtcctga tggtagcagt
    33001 aaggagcaga cgctcgcgtg gttcaagttc tttaagtgga ttaaggagga agagtaatgc
    33061 acatgcacat aggagaaaag atatataaat tgagacggct taataacgaa acaatgaacg
    33121 acttagttaa acaaactgga gtgtcaaaat catggttatc tgatattgag tctggaaaaa
    33181 agcaacgcgt tgattttaat aagatatgtg caatagccaa acattataaa gttagcttag
    33241 aatactttcg ggatgattat ttggatagtg atgaacacga ggatattatt cctttgacag
    33301 gcgaaacaag agttaaatca gtgaaatcgc ttgaacaaag cctaaagtat ctgaataaca
    33361 aacaagaacc taaccttcta gagatcaaac taaaagatac tgactcagta ccagaggttt
    33421 ggtacaaggg tgagaggttg gatgaattgc ctaaaggatt agtagatgtc tcgtatcatt
    33481 ggaagactga tgattttact aatgatgata gaggagcgaa cgacatcacg attcaatact
    33541 tttctagctt taatgataag tatccagata agaaaacaat cggacacaag agagatatgt
    33601 aaatgaaaga agttagacct agagacgaaa tagacaaact atacaagacc aaacgatggc
    33661 gagacctaag gcaagtagta atagctaggg acttcggcat gtgccaagag tgcaagcgtc
    33721 gagggcggaa cacaaggggc acgatcatcc atcacatagt cgaggcgagg gaagacctgt
    33781 cactgttctg gtccgtagat aaccttgaat gtatctgtgt agcttgtcac aacagagagc
    33841 atccagagag atcaggcggg aagaagaaac caaaacctaa atcacatatc gttaaaatgt
    33901 attcaactcc tgaaagataa gtttgcagcg aaatgaaggt agccccccta ctctaaaaga
    33961 ttaaagagta aggcttgaga agaacggtgc tgtccttcct tcgtaaaaag accgcttttc
    34021 aagttttttg gagaaaaagg aaaaagccga ccaatttaag ccggctttgg acgaagctat
    34081 ttcttagtcc atttgtttcc tttttgagaa gtagggggta atcggtcgcc tggatcaatt
    34141 tttacttctt gtccgccttg gacatttcca ccacgaggac ccacttcttt gtaggttcct
    34201 tttggtttgt tgtcttcgcc gggtttatag agttctccca taggaatccc tccttaacaa
    34261 atttcggcac agcactgccg ataacctaat tataaggatt gtgataacga tttcaatcta
    34321 tcttttgaaa gaaggtgata ttatgccgca accagcgaag agtgcaaaat tacaattatt
    34381 aaacggaaac ccaaataaga agaataccga agaactccgc aagcgagcgg ccgcagaaga
    34441 caaattaaaa atggctactg acaaaatcaa accgccgtta tggctagatt cgctaggaaa
    34501 ggataccttt gagtttatcg ccgatgaatt gctgtctgtg gatttaatca gtaatccgga
    34561 cgtccataca atggctctat actccaattg gtattcgcaa tacgtttctt tagaaaaaca
    34621 gcttcgaaaa ctacaacgag agtacaagtt gaactatgcg cttgcgaaaa aggaggcaga
    34681 ggcgagaggt gagccgttta atgaacctaa tgaattaatt ggtaatccgc tctctcggca
    34741 gatggataca gcgtctcgga atctccgttc ttttggcgct gatttaggac tatcaccagc
    34801 agccagagct aagttagcta ttaagatggc tgatgatggt ggtgatgacg atgacgactt
    34861 ctaatatttt ggatatgtct tacacagaac gtgtggacta ttggcaaagc tatcttgagg
    34921 agcaagcttc ttggggtggc tttttaaaat gtccatatcc ggaattgtta actacttggt
    34981 atgcggaacg attaatcgat ggaagcatac cagccagcaa agaaaatatt caagctgcta
    35041 aacggcatat gcgtgatttg cagcgccaag gaacagatga ttttccttgg atctttgacg
    35101 aagaaaaagg tcaccggcct attagatata tcgaaaaaaa atgtaaacca actgaaggcg
    35161 actttggttc gtttgttttg caaccttggc agcatttcat aattggatcc atgtacggat
    35221 gggtacatcg tgatacagga gagcgtcgct tccgcgaggc tcttattttt gttggacgta
    35281 aaaacggtaa gttttgccgt ttaaaaatcg ggcaaaatcg gtgaaaacct ttatttttgg
    35341 ctttctttta gtaataattc gatagcttta tcaagcaatt tagacatagg aaccatagtt
    35401 tcttccgaca ttttttttaa tccttcgtat aaatctttat caattgcgtt agaaattcta
    35461 attctgtttt ttagtccgta tttgtccatg tgtgacactc ctttttattt tactataaca
    35521 tccgattcca ccgcttgcaa ctaccatcaa ttgatggtag tatataaaag aggtgagtag
    35581 attgaaaaaa tacattattt acaaacactt aaataaaata aatggcaaaa tttatatcgg
    35641 tgtcacaaat gatattggca gaaggtggag gagcggtggg attgaatata aaccaccaaa
    35701 gaacgaaacc caacaccaca gaagtttttg gaatgcaata caaaaatacg ggtgggaaaa
    35761 ttttgatcat ttgattattg aagaagactt aacgatgaaa gaagcttttg agaaagaaaa
    35821 gttttatatt gaactttatg actcaactaa taagaaaaaa ggttataaca tcgcaaaagg
    35881 cggaaatggc ggaataatat ataaagttca ccccaaagga atgctaggga aaaaacaatc
    35941 taaagaattt tcttcaaatc acagtaaatg ggcaaagaac cataagaata attgtatgac
    36001 aaacggtgat gttgtatggg gtgttactca cgaacaccca aaaggcatgt tgggaaagca
    36061 ccacagcaaa gaatcaatta tgaaaaagaa agcatatagt ggtgaaaatg ctgtaacctc
    36121 aaaaccaatt gtcgcaattg aacgggatgg aactaaaaga gaatttcaca gcgctaagct
    36181 ttgcatggct tactattcga ttagtacatg tgttttttac cgacttttaa aagatggcgc
    36241 cccttatgtg ataaatccta aagcgaacta cagaaataaa gaaaaaatat tagctatcga
    36301 aggaattatg ttcaaacata aagaagatac cgaggtaagt tagtagattg cgaaaggtta
    36361 ctaactaccg taacgcgtag gagttgaata aatataatgc tcccaagagt gtccgacagc
    36421 agataatttg ctgaaaatgt acgctaaact gggccagaac tgactgaccg atgaaaatga
    36481 gggtgacctc cagagtgcga gataaaaagc tcgcagataa taacaatcga aaacatcact
    36541 aatttcaggc ctttccacat acatggtcgc ttatgatgat gaacaaggcg ccaacgttta
    36601 cgtattggca aatgctcgtg atcaagcaag cttgttgttt gataaggccg cagaaatggt
    36661 caaacaatcg ccggcgctct ttaagaaatt tggtaagcct aaacgatcaa gtattaatta
    36721 tgctcccgcc ttttctaaaa tggaaccacg cgcctcagat agccggaaat tggatgggct
    36781 aaacactcac tttggtattt ttgacgagat ccacgagttt acgaattaca atctgatcaa
    36841 cgttatcaag aaatcaagag gaaccagaaa acagcctctg atagtttata tcacaactgc
    36901 tggatatgta ttagatggtc cgttgatgtc ttattttgag caaggtgtgg actgtttgga
    36961 acatttggaa gatgacatcg atgaacggac tttctattat ctggcaaaac ttgacagtgc
    37021 ggaagaggct gatgacccaa gattatggat caaagccaat ccgaatattt gtctaatgaa
    37081 ttttgttggc atgctagatg actatgttaa ggataaaaaa gatccaaaag aatatgctga
    37141 ctggattacc aagcaattta acttgttttc cgatatcgat gagctgtcat ttgtcgatat
    37201 gcctaccatt aaacgaaaca ataaaaccat cgatattgaa acgctcaaag gtaagaagtg
    37261 tgtcggtggt tttgacttgt ccgaaacgga agactttacc gcagccgttt tagaatttcc
    37321 gcttgaaaca ggcgaggtat tcattttgca acacacatgg atcccacaag ctagatttga
    37381 tcgagataac aatcaagagc gtatcaaagc gtgggagaag gtgggagatc taacgattat
    37441 tcctggtgat tacgtcaatt atgaatacgt cttaaattgg tttgtagaaa attcgaaaat
    37501 ctatgacatt gtaaaaatca attatgacaa ggccaaggcg ctacgattaa ataaagaact
    37561 agaaaatgca ggatttgaaa ccaacgagat tcggcaaggg tttctatcat taggtgggcc
    37621 aatgcaaaac ttcaaggaaa tgctattgga cggtaaggtg attttcaaca attccaagct
    37681 ttaccgatgg tatctatcca acgtcaagct ggtgatggat cgcaactcaa actggatgcc
    37741 gtctaagcag tccaagagta gaaaaataga tggttttgca gcaagtttga acagccacgc
    37801 cgaagtgttg aatatgttgg ttaatcctgt cggaaccggg aaagtaacct attactcgat
    37861 ttccgattta atgaatatgt aagaaaggtg tggaggaatg agtattttag atcgtttgcg
    37921 ttcttttggc cgagcgaagc cgaaagcgag caaacaagag tattttttga atgacccggg
    37981 attgataccg tatttagtcg gaaaagatga aatatcagaa gggatttttt ccgtaattag
    38041 ccgtgtatcg aacgtttttg cgtctctccc tctcaaaatg atagatgtgg agtttggcca
    38101 accggacgac tgtcctgcat acaacttgtt gagcgaaggc cctcgatatt ttacaaagtt
    38161 tgattttttc cgggacgtgg aagttttgag aaactaccaa gggaatgcgt atgtgcagat
    38221 tttccgaaat atcaatggag aagtagcaga tatggcgtta gtaaaacctg gtgcttgcca
    38281 tccagtgatt gatatggata gcggggagct ttactaccaa gtaactgcga ctgacaaagg
    38341 cagttacaag caagttatct atgtacatta catggaaatg cttcacttta aacaaccgag
    38401 gtttggcggc ttggaaggta cagaccccac aaaagtatta acgaataccc tcggatatga
    38461 tcgagaagtc cgaaaaatct ctttaagtca gcttaaagga agtaatgaag ggctaaaagt
    38521 taagtttgct agcaatatgg atgaagaagc taaaaaagct acagttaaaa acattgctga
    38581 tttttatcga caaaacggtg gactacttgt ggaagaaaac ggtgtagaaa tcgaacgttt
    38641 acaacgagag ctggtagaca gcaagctttt agatactgat aaagtatctc gctccagaat
    38701 cgctatggtc tacaatgtgc cggagcattt catcgggaat aaccagtcga gttactcctc
    38761 tcaggaacag ctcaatatgg aatttttgac gtacaatcta gtaccgaccg ttaatcaata
    38821 tgaagcggaa ctaaataaga aaacactatc gagaaccgaa aaagcaaaag gttatcgata
    38881 taagtttaat gtcgcaagtt tgctaagagc tgacactcag gcaaggggac aattctatca
    38941 aatcatgcga cgaggcggag catactcagc gaatgatgtc cgaagatttg aggacttgca
    39001 accgataaat aaaaccggta tggatgatta ccatatttcc ggagacctat atccaatcga
    39061 tatggatcca acattaagaa aaacaacctc gtctaaaagc gtagccgaaa acggttaggt
    39121 ttttttagtt tgcaccgaag ggaggtggaa aaatgaaaaa agtgacgtta agcggcgatg
    39181 tcgtggataa cgataccgcg tggctttatg actggtttgg gatcaattgt atctcaccag
    39241 ggaaaatttc tgccgctctt acagaagcag cgggggatga agtagaactt gatatctcat
    39301 cgaacggtgg ggatgtccta gcggcaagcg aaatatatac cgctatccgt gcctatccgg
    39361 gaaaggtatc cggaaatgtt gtgagtattg cagcaagcgc tgcaagtgta atcgcttgcg
    39421 cttgcgaacc gcttagaatc tcacctacgg cacacatcat gattcataac gcatgggtga
    39481 ccactagtgg caacgctgag gaattaaaag ccaatgcaga aatgttaagc agtgtggatg
    39541 agtctattgt taatgcttac gagatcaaaa caggactcga tcggaaaaaa cttgctgatt
    39601 taatggcgaa agatacttgg ttaaatgctc aaacagcagt agcagaaggg tttgcagatg
    39661 aaattatgtt tgcagaagca ccagtaacgg tactcaatgc ctctcaaccg gttattccaa
    39721 aaaacgcagt aactaagttg aaaaatttaa tactcaaagc ggaagcaccg caagaagaaa
    39781 cactcttaca gaaaaaacta aaagccttaa atggagggaa aaacgaatga atttagaaca
    39841 attaaaaaat gcgtgggtcg aggcgggaag taaagtctct gacttaaatg cacaactcaa
    39901 tgcagcattg gttgacgatg aaaaaacaga agaagatgta gtaagtttgc aagcacaagt
    39961 aaaagcagca cgggctaaac gggacggatt gaaagagcaa gtggcaaata tggaagccga
    40021 acaagtctta aacgtcaaaa aagaaccatt agataaaaaa gatgaaaact tgaaaaacaa
    40081 gtttatcaaa gactttaaag cgatggtcaa tggtgatcct gctattatgg ctactttgac
    40141 atctaatacg gatgaatctg gtaatgctat cggattgact attcctgtag atgtgcaaac
    40201 gactattcat actttggttc gtcggtttga ctctttgcaa gaatacgtaa acgttgaaaa
    40261 agtgaccact accagcggtt ctcgcgttta tgaaaaatgg tctgatatta caccgttgac
    40321 tgctttggat actgaagacg gtgaaatccc agcaaatgat gatcctgcac tttacttgat
    40381 caaatacttg atcaaacgct atgcaggtat ttccacagta actaacagct tgctaaaaga
    40441 tactgccgaa aacattttgg catggttgtc taaatggatc gcgaaaaaag tagttgttac
    40501 tcgcaataca aaaatcttgg cagctattga tggaatcaaa gcggcacaaa agaaagatgt
    40561 tacagatgtt gatggaatta aagatatcgt aaacgtccaa cttgatccag ctatcgaagc
    40621 tacatctatg tttattacaa accaagatgg cttcaatgtt ttagataaag tgaaacgtgc
    40681 tgatggatct tacttgttac aaaaagacgt agcttctgca actggatata ccttcttagg
    40741 taaaccaatc aagaaaattg cttctcgttt cttgccaaac aaaggtacgc aagctactcc
    40801 taaatatcca ctgtacattg gtgatctgaa agaagccgtt acattgtatg atcgcgaaaa
    40861 catgagcttg ctgacaacga atattggtgg tggagctttt gaaacagaca ccactaaagt
    40921 acgcgtcatc gatcgcttcg atgtgcaact agttgatgat gaagcggttg ttttggctac
    40981 ttttacaact attgcgaacg agacaccggc gaaagtttaa ggagctgatt ccttatgatt
    41041 cttgatccta aaatggattt aggcgaaatc aaaaacgcac taaagattga taccgatgat
    41101 gacgatgtgg aagtaagccg tgcggcacaa gctgcaattg catacattag aggggctatc
    41161 gggaatgata agccctcttt ttataagcaa gaaaatgaaa cggttgatct gattaattta
    41221 gctatcctgc aattagcaga tcattattac aacgctcgat cagcaaccgt gagtgggaac
    41281 ttgcgagagt acgatttagg ttttacaagc ctaatcttgc aactcaaagc aagttatttg
    41341 ctttttgtgg aggaggagta gcgtatgccc cttatccaaa caggaaattt aaatcaacgc
    41401 atcaagtttg tccgagatac gacggttaag gatgaggacg ggcaagttgt cccgacttct
    41461 acaaccattc ttacttgctg ggcaagtgtg cagacacaac gcctgaacga tattaagacg
    41521 tcgattggta cggctttgga aggaacactg acgttcatta tccgctacca acaaaaatca
    41581 gagctgacca atgatatgaa agtgcgttgg aatggaaaaa cgtttgaaat cattacgatt
    41641 acgaaaggcg agtttgcgaa ggacttcacg acaatcattg caaaagaggt ttcaaaatga
    41701 gtgtagaagt cgatgcaacc gaagtgtaca aagcgcttag ggaagtaaaa gcaaacgttc
    41761 aacgagtgga aagcccagca cttagaaaag ctggggagta cgctcaagaa gagttacgac
    41821 aaaacacacc ttactgggat ggaacgaagt caaacggtaa acgtggttcg tatatgcaag
    41881 aacatgctaa gaaccatgtg gttacaagct cgataaaaaa cggattggta gaagtcggct
    41941 atgacaaaga tgtttcttgg cggatgcact ttatcgagtt cggaacaatc aaacaacgtc
    42001 caaaaggttt cgtacaaaaa acacaaaagc aaatcgaaaa acaagtaaca caaatcattg
    42061 ctgacgaagt aaaaaggagg ctaggacttt gaaaacggca gtatcacaag tctattcaat
    42121 tctgaatagc aatgaaaaaa caaagaacat tgatttttac accaatagtg ttccggaatc
    42181 agctcaaaca gtacctagcc ttccagttgg cagaattaca gagatatccg gcaactatga
    42241 agattttgca agcaacaatc ctttgaccat tcaatttaac gtacaggtag atgtatgggt
    42301 gtcaaccatg aaagaggttg atgcctttta ttttgccctt gatgaggtta tgagggggaa
    42361 tggttggcaa tgcgcataca cggaacaaac agatgacgag gacttggaag gtgcaaagcg
    42421 gattatcaaa cgatatgtag caaatatttc actaaattaa aaggagagaa aatagatggc
    42481 aacagtagga ttcgagagcg tcatttttgg cgtaaaaaca ggtgcaggcg gcactctaaa
    42541 agaattagta gcagataagt cgaaaggcgg agcgatcgaa gctaaaatta ctggattagg
    42601 cgcaacttct aacacaacat acgcttcaaa cgtaccgttc ttcattgcaa gtaaaggggt
    42661 ttcgtcgcca aaagttacgc ttgacgtggc agacttaatg gataacggca tttacagcga
    42721 aatcattggt gctaaaaccg tggatggtgc aaatgtaatt ggttcagaaa ctgaagcgcc
    42781 ttacgtgtcg gtagtcatgg ttacagcgaa caaagaagga aaacgcttgt tcatgggatt
    42841 gacaaaagga aaattcagtc atccagatat cgacatgaaa acagctgaag acaaaggggt
    42901 agaattgcaa accgattcca tcgaagggga attcatttct gatgaacgtg gctatgtata
    42961 cttaacagcc gtagaatcag aaggaatgac cttacaaaaa ttcaaggact tggtaaataa
    43021 caaagagggg gagtagttaa ccctgcatct acaccaacga cagatacagg gacaccaaaa
    43081 gaaccagaac caaaaattga tacacaaggt tagccatttt tggctagcct tattttttgt
    43141 aaaaacaagg aggaaaacaa atgattgaat tgcaattgaa acttgacgga aagaaaaaaa
    43201 cattcaaaca acaagatatt tccgcacgtg caatgcgtga gtgtatcaaa ttttacgaga
    43261 aagcggaaaa agcagaccta actgatttag aagcgattga ttcaatgatt gcaattacag
    43321 cagatatttt ccaagatcca gcagttacat ttgatgctat tttagacggt ttgactgcga
    43381 gcgagttagt accggcatta gaaagtgttt ttgaacaaat aaatgaactg ggaaacaatg
    43441 aaaaaaagca gacggcgagc aaaaagaaat aagtttttct gaagctagga aagcaatgga
    43501 tcaaatctac aaagatttaa tcgaagcagg ttggacgatg agagatgtgg acgaagccga
    43561 ctatcattat ttgttacacc tttttggaga agtggagagt ggcgaagaat atgtagatgg
    43621 tgctgatttc atcaaacaat ttttatcggc tgaagactta gtaaaacttg aggaaggagg
    43681 taaataatgg caggaaaagg acaaccggca ggaaatatca agctagggat tagtttagat
    43741 agcactagtt ttggtaacac gctggacgaa atcaatgcga aagtcaaaca agctgagtcg
    43801 aatatgcgtg ccaatctaaa ggcttatgat tcagcaggac gttcatacga agcacttagt
    43861 caaaagacga aagacttgtc tacggttatg gaagggcaaa acgccaaagt aagagaatta
    43921 acaaagcgcc gtgatgaagc gattagcaag tatggcgagg aatcgaaaca agttgctaac
    43981 cttaacacac agataaacaa tgctaccgca aaatataatg cttacagtcg ccagttgaac
    44041 gacacaaaaa aagaattggt gtattccaaa acagccgtca atgatttatc taatgaaatc
    44101 aaagaaaatg aacgacaaat gaacgccgaa gtaaaagcgt tgaaagccgc tggtgatgaa
    44161 tctggtgctt ttgaagcaaa acaaaaaggg ctagccaaac aaacggaatt atccgagaaa
    44221 gctatcgaag aacagcgcaa agttgtgaaa ctgatggctg atgagtttgg cgattcagca
    44281 aatgaaaccg aagatgcaaa aagggcatta gaaaagttag aacgacaaag ccaaatatct
    44341 agcaggcaat tagaagcact caaaagctcc agcgatcaat caggaaaaga aatagaagat
    44401 tttggcgaca agtccacaag gtcagctagg aaactggacg gactaaaaga caaattaagc
    44461 tcgctaaaaa gcgcattttc gtttggtgca gttgctggat tagcgcataa cgctattagc
    44521 agtgtagtaa gtggcgtgca aggtttggtt ggcgaagcag taaacgcatc ggattcattg
    44581 atgaagtttt ccaaaaccat ggagtttgct aactttggga agtcacagat agaaagctcg
    44641 aaaaaagaaa tgaaagacta cgccgataag acggtttatg gtttagaaga aattctgaac
    44701 acaaccgcac aattggcatc taatgggatt cctaactata cagaactaac caaggcggca
    44761 ggtaacttga atgccgttgc aggcggttct agtgatacat tcaaatccgt tgccatgatg
    44821 ctaacgcaga cggcaggagc tgggaaacta acaactgaaa actggaatca attagcagat
    44881 gcgataccgg gtgcttcagg attgttacaa gacgctatgt tgaaaaacgg agcttataca
    44941 ggaaacttcc gtgatgcaat ggcgcaaggt caaatcactt ccgacgagtt caaccaagca
    45001 attgtacagt taggtatgaa tgacggagca gttaaggcag ccacttctac agacacattg
    45061 agcggttctt gggagcagat gaaatccact gtaataaatg ggctacaaag tattatagaa
    45121 aaaataggcg ttgaaaatat cactggtttc atcaacagag taacaaaagg gattgaaaat
    45181 tctattccta aaattactca atttataggt tggttgagag atattggaac gtggatcgtt
    45241 gaaaatagag agccactaac atggattgtc ggaatcatag gcggaattac attagcagta
    45301 aaagcattga acgtagcaag tatgttgctg gcaattactg gcggaacatt ggcagcccct
    45361 tttgtggcga ttggtgtagc attaggcgca ctagcaggtg ctttggtgat agcttataca
    45421 aaatctgaaa catttagaaa tatagtcaat gcggctttta cagctgtgaa aaacgtagtt
    45481 atgagcgtta tcaataattt ggtggaatac tacaaaatgt tgtggagcgt gttgcagtgg
    45541 ctttgggaaa aaataaaaga atgggcttca tggattggca ataaattcat tgaaatgaag
    45601 aacagcgttg tgaatacggt tcagaaccta tggaatggcg tgaaaaactt cttcagcaat
    45661 ggcgttggag atacgtggaa taaggtagtc ggttgggtaa aaaacatttt caacaaagca
    45721 actgaattga agaacaacgt ttctgatgta atcggtaacc tgtggaatgg tatcaaagac
    45781 acattccgta gaggtatcga tacggtattc aattggtttt cagaactacc aacgaagatg
    45841 aagaatgcca ttattggcgg taaaaacgcc attgttgatg cgttcaaaag tattttcaac
    45901 gcagcactta aagcgatagg taaaccagtt aacgcaatca tccatggagc ttcatgggta
    45961 ctagaaaaat tgggcgcaga acctctacaa gaatgggatg taccacaata cgctacaggt
    46021 acaccagcag gtgggcaccc aatcaatggt ccaatgatgg ttaatgatgg acgtggagca
    46081 gaaacagtta tcacaccaga tggtagagcc ttcattccta aaggacgcaa tgttgttcta
    46141 aacgcaccaa aaggaacgca tgtcttgaca gcagaagaga ccgcgcaact tcaaggttct
    46201 aaagctccta agtatcgtta caaaaaaggt actaacttct ttggtaacat gtgggatagt
    46261 gtgaaaaagg ttgctggtaa tgtaggtcac acaattaaaa acgtagtagg tgacgtgtgg
    46321 gactttattt cagacccggg agcgttagct agaaaagtac ttggggggtt agatgtatta
    46381 ggtggattga caaagtatcc attagaagta ggtaaaggca tcctatctaa agcaacaagt
    46441 gcactgactg aaaaaatcac tgggttgttc tcatctggta acttagatac ctccgtagga
    46501 acaaatggcg tctataaata tttggctgat gttgctaagt ctgtgatgaa gaaattccca
    46561 ggatttgtgg caactagtgg gtatagacca ggtgaccctt attcacatgg taaacgtaat
    46621 gccattgata ttgcattacc aggcgtcaca ggaggctcac ctagatacac agaagcagca
    46681 aactacgctt ttgacaaatt cgcttccaaa attggttacg taatcactaa tgggaaagtt
    46741 cgtgaccgtt caggacaatc aggtcaacca gcaactggtg catgggagcc atggcccgat
    46801 ggagatcact atgatcatgt gcatttaaac ggtgtgaaag acccacaaaa cactcaaatt
    46861 tcaggagata gcgtgggagg cagtggggta gaaagatggc gcaatgtagc aattagagcg
    46921 ttgaaaatga ccggtcaata cagtactgca aacttaaatg cattactaaa tcaaatgcgt
    46981 acagagtcaa atggtaatcc taatgcagtt aacaattggg atattaacgc caaaaatgga
    47041 acaccatcaa aagggttgct ccaagtgatt gacccaacat tcagacagta tgcaatgcca
    47101 ggattcaaca gcaatatttt tgacccacta tctaacatct tagcttcaat cagatacgca
    47161 ctatcaagat atggctcact aacaaatgcc tatcgtggag ttggttacgc aaacggtgga
    47221 attgtaaacc aacatcaaat tgcggaaatc gcagaaggaa acaagccaga aattattatt
    47281 ccgttagata aggctaaacg atcaagagcg atgcaattat tgtctatcgc aatggacaaa
    47341 ctaggagttt ctcctaaaac ttatggaaat gcgacaactg tttccagtga ttacgaaatc
    47401 ctagaagcca ttgaaaaaca ggcggcaaca acgaatcagc tgttatcgtt attgctggca
    47461 tttttcaaag gaaatagccg aactgataga gatttagctt tagatattca gaagatcttg
    47521 gttaggagga tgtagatgcg aactgtatta ctgagaaata gaataaacga agaaattgat
    47581 ttgtccacag aagactattt tgctactggg ttgagtaata tggggtttga agtaaaaaaa
    47641 gaacatgtgg gacagtgggg gaatttcaga gaaagcagtg agagcgttga aatatctgaa
    47701 tttcagtcat ctgtaatcat ttctgtgcat gggtttcgag aaaaagaact gtacaattca
    47761 cttgtgcagt tcctatcgga aggtccgttt gaactggaat ttgcttttga cggcgaaaca
    47821 atggtaagaa gatgcagtct aaaatcttta agtaagactg aaatcgatcc gaaaacatcg
    47881 ttactaacag acactttaga actatatttc acatcaaact ggtattccgt aaaacgcgaa
    47941 aaattaattc agcgaccgaa tgtagtgaaa acacgtggta aggtttttcc gtataaaaga
    48001 tcttatatct acactcaaaa cttgtgggag aaaaaaggtg tattcaaatt caataataat
    48061 tctgtatacc taacgaatag caaggaacgg atgtccccgc taaaaattcg tgtgattggg
    48121 aagtgttcaa atccgtattg ggaagtaatc caaaactcac aaatcatcgc aacggatgga
    48181 tacttcatag atatgactga aacacagact ctagaagtat caagcctttt tgaagataca
    48241 acggcgattt taaaagatat tgcaggggta gaatcttctg tctaccaaca acaggattat
    48301 acaaaaacta attttgttca ggccccaact ggagaattca gcattgtgtt tcatgttggg
    48361 ggggcagacg tagagattga gttatacgag gagcgtgatc tgttttgatt ttagcagtca
    48421 cgctttttca tcgtgattta atgctttata acgaacattt attctctaac agttttgaat
    48481 ttggtgtgga cgagatcaac gaagaggcta gtagctttac gatggataag tacgttcctg
    48541 taaaaacagg cgattttcta ctagcaaaat atattcctag tggaaaattt gcatattttg
    48601 gtgtaattac gtcgcaggaa gacgaaaaaa tcagttgtaa aagcttactt agtttagctg
    48661 atagtgagat accgactgca cgtgtgtcag gagataacta cgaagagcat atccggcggt
    48721 tgattgaata ctatttgctg aatgatccaa cgaaacaact aaaagatatt ttagacgtca
    48781 aagcagaaag tgcgacctct cattcgtatc aagctaccga tacgaataaa cacaagttaa
    48841 gtgcgtatat tctcaatggt ttcaaaaaat acaacgtaaa atggtatttc aagggaattc
    48901 aaaacagaaa aatttataca ggcatacgtg ctgtaaatga atcaatttac attaaagaca
    48961 attcttctga atttagcgat tgggatgtgt ttgttcaggc gccgggcgct ggaaacgaga
    49021 acaagctatt aatcgttgat aaagcaatga aagatataga gaaaccgata atactgtcaa
    49081 catggtattt ggacgaagaa aacaatttga cacaagatgg atcgaaagaa aatatcacga
    49141 aaccaactgt caatttggtt aatatctacg atcaaaccgc agaagataaa gcatcctacg
    49201 aagatgtggc aaaatcagag ttgaaaggca atacgtattc gcatgaaatc aaagtgaacg
    49261 ttgtaagaaa tgcaaaaaat ttgaatgtcg aaacgattga aacagggatg tttgccacga
    49321 tttcttataa aggaaagata tacaagtcgg ttttgacagc ttggcgaata tcaagtgata
    49381 aggagtttgt ggaattgact ttcggaaaca taagaagtcg ttttatggat tattttgaag
    49441 ataatggggg ataaaaaatg gttagcaatg tggatggata tcaatttgaa aacgtgaaag
    49501 taagcgcaga aaatgatgct agactttatc acgttttata taatcggaaa aatcaggtta
    49561 ttgatggtta cgatcagtct atgaatttat cttcaagcgg attaacagta aaagttgctg
    49621 caggagcagc gattattcaa ggtcgtatgg tcgttgttcg acaagaagaa agtataacag
    49681 ttccagcaaa ctcaagtggt tatatagcat taacagtgga tttgacacaa gaagttatac
    49741 ctggatctat tcttccagaa tcggaagaat atgaatggac taataatcaa gtcaagctag
    49801 aatttataac aaaagttata aaaggtaatt taaacaatgg tgataaggtc tataatttac
    49861 cactatgctc agttaattct actggatcaa ctgtttcaat ctcaaagata tcggatagtt
    49921 acgagctgac tctctctaaa ggagaaattt tgtggagggg gactgcgtta atgcatgata
    49981 ctcaaactgt ccaaccttca aaaaaaattt ggcagacagt ttcaggtttt ttgttagtat
    50041 ggctcccata cgaaaacggg caagcaattg aggatagata tgtaactacg cctttttata
    50101 aggagcgtgt aacttttact aatgttttag gagaaattgt ttcaggattt gatgactatc
    50161 acaaaaagtg gtttagtaaa cgaataaact ataattcgaa tactaatata tttacaggtg
    50221 cagcaagcaa tgcaagtgga gataacgcaa atatggtgct taggtatata gtttcttttt
    50281 agttaggagg tatggaagag tggcaaattt agaaattaaa ttatctgcaa ataagagaca
    50341 gccttatcta cgtcaccgtg ttgttggtag agttggtgat ggggggctca caacaatcaa
    50401 tgtacaactt cttgaggaag atgaaattac accttttgta attaatctaa acggtacttt
    50461 gaaatttgtg ggcgaagttt caaacggtaa ctataccgag ggagaaccag aaataatcga
    50521 ttcgactaat gggttaatta gttacacgtt cactaagtca aatttcagca cgagtcatca
    50581 attcaaacaa gcatattttg aatatgtaga tcctaacggc aaaaaagtaa cttttcagaa
    50641 cttcatcata gacgtgttag aacgagtgga tattaattcg gagcaagcga aatactatat
    50701 ttcttcattg gaaaaattac agagtgaaat gcaaaccact ttcaatcagt tcattagtga
    50761 taaacaggtc caatacgatc aaatctactc gaaatataac gaattagtaa gattgataaa
    50821 tgaatcagat aagcaagtaa atgatcgtat tgatcaaacc aatcagcaaa tcggcgatct
    50881 cggcaagctg aaaaagatgt acagtaacag catcgacttt gggggctatg attattcggg
    50941 gaatccgaat ttgttaagta agctatcata cgacttaatt gaaaatcaaa atacttcagc
    51001 tggaacactt tctaagggtg aaaactcgtt taaatataat aagatatcag ctgaagtgga
    51061 aggtggagta gagttatatt ataaacgaag aggtatagct aactggttac cctctaataa
    51121 aacgcttgta atgactgtta agcttagagc tggagcggac tatagtccag ttgacggaaa
    51181 acttatactg attagatata ggtatgttga cagtggaact ggcaagattg ttttagactt
    51241 acctattaac agtaattcga taactcagga atggaaagag tttagtatta ctggaactac
    51301 tccaacattt agcccacaag cataccatcc ttggatacaa tttagggctc aagatggggt
    51361 acttggggaa atagaaatga gctatgacat caaaatcgaa gaaggctcta cagctacacc
    51421 atatcagcct aacttacttg atgactctta ctggctaggt aaaacaccgt tgggtgagaa
    51481 tttgataacg aatgccaagt tcccaattat aacacgaagc aatcctattt ctggttttga
    51541 tatttcagaa gaattaataa taggtgaaac ttatacggta tctttaaaag ggactaagcc
    51601 agctactaaa gaattccact tatactacgg agaagcaaat taccagcagt tgcagtatca
    51661 ggcaacttta cttcctgtgg aaggattaac agatgtgtgg agtgccactt ttacagccag
    51721 aaatactagt gaaacgacaa atttacttag agtagcgtta tggcaaaaac ctaattctgc
    51781 aacatatgat actgttcaaa ttgactggct caagattgaa aaaggcgaca cacgcacacc
    51841 aaatatcagt cagtttaaat actttggtga aggcttgaaa gacagtaaca atccgaacga
    51901 ctacagctgg gatgtcacac cagagtatac tgaaaaaagc ttgaataata cggttagtct
    51961 gactgaaccg caatccatcg aaggattgaa aaatttcgaa gatggtattc aatcaaaagg
    52021 aaaatctgta ttgacatcag acgacaacaa atatgaagtc gtaaccttaa cagttacaaa
    52081 cgggaatacc ggatcagcaa agctttatcg tgaaggaaaa accgtcacta tttatttttt
    52141 tgcgttgaat gggaaaagca gtggtggaaa tgattcaacg atactaacaa ttccagaagg
    52201 ctatcggcca ccaattagtt ttgagcaact ggttggctcg atagaccgtt ctactttgaa
    52261 cagtgctcag ttatctattg gtgcagatgg agccattaaa tggcgaagaa actcaagtta
    52321 tggatcggat tatacctttg caattactta cacgatttag aaaggcgtga atcgatatga
    52381 aggcagcata tagaccaatt gaaccttacg gattcgagca aatcattgtg aatgatgaag
    52441 aacatttacc ggaagaatgc acagaagtcg aaccaccgat tccaaattgg aaaccgaaat
    52501 tcaattactg ggagggaaat aaatgaaaaa catttggaaa tatggccgta ctggcggaga
    52561 gtatgcagga aaagtattgg acgacatgct tgtatccgtt ccttacacag atcagccacc
    52621 gctcgaaggg gttcgttcag atggcgaacc gctaacgatt gctgatcaga tgtttgatcc
    52681 taaattgaac caatggatta ttttagcgaa cgcgttagat cacaacgatt taaacaatct
    52741 caaagcgatg tacgaggctc ttgaacatga aaacggcaac ctaaaacagc taaatgccaa
    52801 actcatgcta agcgatgtag cgattaaaca ggaaaatact gcattgaaag aaaaagctga
    52861 cagtttagca caaatcaatt caaaaatgat gcttgcttcg ttacaaaata gcaaagacat
    52921 ttcagaaatt aaagagcaac taaatccagc ttcaaaggga ggtgagtagt atgtttagtt
    52981 ttagcgatgt gaaaatgatg tatgattggg gctgttttac tgacgatcaa gttcgtctat
    53041 tcgttccact atgtatcaca gatgaagaag cagatagaat tattagcaaa gaagagagcg
    53101 catcttaagt gatgcgtttt tttgttggaa agttggtgga acatgaaaga agaagcgctc
    53161 caagacgttg tggagagatt agtaagaatt gaaacaaaat tagacaacta cgaatcactt
    53221 agagaaaaag ctgatagtgc aaaagatttg gcagacaaag cctactcagt agcgctaaac
    53281 aacgcagaag atatcaaaga aatgaaagcc aataataaat ggtcgtgggg ttacatgatc
    53341 ggtttaggca ttacgatcat tggctatttc ttgactaaat tgtaaaggag ttaagaagaa
    53401 atgattttac ccgataagta ttatcaagtc attaaatgga cggttttaac agtattacca
    53461 gctgcatctg ttttagtagc cacgttagga aaagcgtatg gatggaatgg aacagatatg
    53521 acagtactca ctatcaatgc agtagcaaca tttttaggcg ttatcactgg tgtgtctgca
    53581 tataacttaa aaaaatagga ggaaaaaaat gaagaagaaa atcattttat cattgagcct
    53641 aataatggct ctttttttat tgccaattaa cgggttcgcc tatacgatca acaatgaatt
    53701 taatttaggt gtaaatgaag gtagctc
//
LOCUS       DAFGZE010000013        53723 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.013, whole genome shotgun sequence.
ACCESSION   DAFGZE010000013 DAFGZE010000000
VERSION     DAFGZE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.013"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            160..840
                     /locus_tag="LTU54_000790"
     CDS             160..840
                     /locus_tag="LTU54_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL8295524.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            930..1964
                     /locus_tag="LTU54_000791"
     CDS             930..1964
                     /locus_tag="LTU54_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HBL8295525.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            1978..2313
                     /locus_tag="LTU54_000792"
     CDS             1978..2313
                     /locus_tag="LTU54_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295526.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            2297..4777
                     /locus_tag="LTU54_000793"
     CDS             2297..4777
                     /locus_tag="LTU54_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295527.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            4833..6392
                     /locus_tag="LTU54_000794"
     CDS             4833..6392
                     /locus_tag="LTU54_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295528.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            6389..7753
                     /locus_tag="LTU54_000795"
     CDS             6389..7753
                     /locus_tag="LTU54_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL8295529.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            7924..8994
                     /locus_tag="LTU54_000796"
     CDS             7924..8994
                     /locus_tag="LTU54_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295530.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            9009..9677
                     /locus_tag="LTU54_000797"
     CDS             9009..9677
                     /locus_tag="LTU54_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295531.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            9697..10275
                     /locus_tag="LTU54_000798"
     CDS             9697..10275
                     /locus_tag="LTU54_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8295532.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            10676..11587
                     /locus_tag="LTU54_000799"
     CDS             10676..11587
                     /locus_tag="LTU54_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL8295533.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(11965..12882)
                     /locus_tag="LTU54_000800"
     CDS             complement(11965..12882)
                     /locus_tag="LTU54_000800"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HBL8295534.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            13084..13521
                     /gene="zapA"
                     /locus_tag="LTU54_000801"
     CDS             13084..13521
                     /gene="zapA"
                     /locus_tag="LTU54_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBL8295535.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            13579..14127
                     /locus_tag="LTU54_000802"
     CDS             13579..14127
                     /locus_tag="LTU54_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HBL8295536.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            14242..16602
                     /locus_tag="LTU54_000803"
     CDS             14242..16602
                     /locus_tag="LTU54_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBL8295537.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            16689..17003
                     /gene="trxA"
                     /locus_tag="LTU54_000804"
     CDS             16689..17003
                     /gene="trxA"
                     /locus_tag="LTU54_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBL8295538.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            17185..18963
                     /gene="uvrC"
                     /locus_tag="LTU54_000805"
     CDS             17185..18963
                     /gene="uvrC"
                     /locus_tag="LTU54_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBL8295539.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            19108..20007
                     /locus_tag="LTU54_000806"
     CDS             19108..20007
                     /locus_tag="LTU54_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295540.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            20000..21262
                     /locus_tag="LTU54_000807"
     CDS             20000..21262
                     /locus_tag="LTU54_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295541.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            21423..23321
                     /locus_tag="LTU54_000808"
     CDS             21423..23321
                     /locus_tag="LTU54_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBL8295542.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(23651..24370)
                     /locus_tag="LTU54_000809"
     CDS             complement(23651..24370)
                     /locus_tag="LTU54_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBL8295543.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            24707..25039
                     /locus_tag="LTU54_000810"
     CDS             24707..25039
                     /locus_tag="LTU54_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295544.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            25026..27029
                     /locus_tag="LTU54_000811"
     CDS             25026..27029
                     /locus_tag="LTU54_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBL8295545.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            27048..27518
                     /locus_tag="LTU54_000812"
     CDS             27048..27518
                     /locus_tag="LTU54_000812"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBL8295546.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            27539..28123
                     /locus_tag="LTU54_000813"
     CDS             27539..28123
                     /locus_tag="LTU54_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HBL8295547.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            28138..29124
                     /locus_tag="LTU54_000814"
     CDS             28138..29124
                     /locus_tag="LTU54_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HBL8295548.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            29114..29425
                     /locus_tag="LTU54_000815"
     CDS             29114..29425
                     /locus_tag="LTU54_000815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBL8295549.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            29447..31228
                     /locus_tag="LTU54_000816"
     CDS             29447..31228
                     /locus_tag="LTU54_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBL8295550.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            31221..32597
                     /locus_tag="LTU54_000817"
     CDS             31221..32597
                     /locus_tag="LTU54_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBL8295551.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            32604..33239
                     /locus_tag="LTU54_000818"
     CDS             32604..33239
                     /locus_tag="LTU54_000818"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBL8295552.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            33269..34624
                     /locus_tag="LTU54_000819"
     CDS             33269..34624
                     /locus_tag="LTU54_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBL8295553.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(34816..36210)
                     /locus_tag="LTU54_000820"
     CDS             complement(34816..36210)
                     /locus_tag="LTU54_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBL8295554.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            36302..37273
                     /locus_tag="LTU54_000821"
     CDS             36302..37273
                     /locus_tag="LTU54_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBL8295555.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            37270..38052
                     /locus_tag="LTU54_000822"
     CDS             37270..38052
                     /locus_tag="LTU54_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBL8295556.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(38221..38592)
                     /locus_tag="LTU54_000823"
     CDS             complement(38221..38592)
                     /locus_tag="LTU54_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8295557.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            38726..39169
                     /locus_tag="LTU54_000824"
     CDS             38726..39169
                     /locus_tag="LTU54_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBL8295558.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="LTU54_000825"
     CDS             complement(39212..40021)
                     /gene="yidA"
                     /locus_tag="LTU54_000825"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBL8295559.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(40040..41407)
                     /locus_tag="LTU54_000826"
     CDS             complement(40040..41407)
                     /locus_tag="LTU54_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL8295560.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(41391..42221)
                     /locus_tag="LTU54_000827"
     CDS             complement(41391..42221)
                     /locus_tag="LTU54_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBL8295561.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            42384..42821
                     /locus_tag="LTU54_000828"
     CDS             42384..42821
                     /locus_tag="LTU54_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBL8295562.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            42897..43517
                     /locus_tag="LTU54_000829"
     CDS             42897..43517
                     /locus_tag="LTU54_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HBL8295563.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            43706..44212
                     /locus_tag="LTU54_000830"
     CDS             43706..44212
                     /locus_tag="LTU54_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBL8295564.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            44229..45128
                     /locus_tag="LTU54_000831"
     CDS             44229..45128
                     /locus_tag="LTU54_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBL8295565.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            45215..46099
                     /locus_tag="LTU54_000832"
     CDS             45215..46099
                     /locus_tag="LTU54_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL8295566.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(46291..46362)
                     /locus_tag="LTU54_000833"
     tRNA            complement(46291..46362)
                     /locus_tag="LTU54_000833"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(46327..46329),aa:Arg,seq:ccg)
     gene            46422..47756
                     /gene="rpoN"
                     /locus_tag="LTU54_000834"
     CDS             46422..47756
                     /gene="rpoN"
                     /locus_tag="LTU54_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBL8295567.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            47905..48996
                     /locus_tag="LTU54_000835"
     CDS             47905..48996
                     /locus_tag="LTU54_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HBL8295568.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            49037..50038
                     /gene="gap"
                     /locus_tag="LTU54_000836"
     CDS             49037..50038
                     /gene="gap"
                     /locus_tag="LTU54_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL8295569.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            50148..51338
                     /locus_tag="LTU54_000837"
     CDS             50148..51338
                     /locus_tag="LTU54_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBL8295570.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            51442..52197
                     /locus_tag="LTU54_000838"
     CDS             51442..52197
                     /locus_tag="LTU54_000838"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBL8295571.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            52313..53611
                     /gene="eno"
                     /locus_tag="LTU54_000839"
     CDS             52313..53611
                     /gene="eno"
                     /locus_tag="LTU54_000839"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBL8295572.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT        17826 a         9357 c        11351 g        15189 t
ORIGIN      
        1 ttaataaaaa aggaagaata ttcatgtaaa cgattttatt gtttttttct cttttataca
       61 aaaagggtca taatttcaag taagcggatg aattcatgag gattttttgc atactgtctc
      121 tactatactt aggtcactaa gaaaaaggag tgatttatta tgaaaaagaa catgattggg
      181 acattattac tttcaacact tcttctaggc ggcttggcaa caccagcttt tgctgagggt
      241 caagcgactt caaaaggaga tattaccttt actgaaccaa caaataccgt ggagcctctt
      301 aaccctactg atccttcaaa accagtagag ccagcagatc cggaaaatcc agcaactggc
      361 cagacaggtc ctttaacact ggatgtagtt cccgaattgc catttggtac acacgagatt
      421 gaaagcggaa caaaaacgta tcaagtagac gcttcaaaaa acgatacacc ttatctccaa
      481 gtatcagatc gtcgaggtgt aggtgcagat ggccaagcac aagggtggaa cgtgacagtt
      541 tcggtttcag attttgtaaa cggttcacaa gttttgcaag gcgctgaatt agattttgga
      601 acatccactg ttaaaagtac gtcagataac gaatccactg gaccaactag tcaaactgta
      661 actgggttga gtaaagcaag tgcagcgaca ccaatattta cagcagcaaa agatcaaggt
      721 ctgggaacat ggctttctgt ttatgatcca gcaaatatca cactgaaagt gccgaaagca
      781 gcagctggaa cattcacggc tgatttgact tggaacctcg tggctggccc agtagcatag
      841 agtatgaaat aaattcaaca aactgcggtg aaaatactat aggtgaagga ttttttaccg
      901 cagttgttgt tgaaaggatg tggtggacaa tgaatcaaag taaacgactg tttgtttcgt
      961 ttttttctat tttaagtcta tttttcattt ttccgtcaat cagccaagca aaggattcag
     1021 caggagactt tggaatcaaa ccggtatttc cagagaacca aatagataaa gcaatcggtt
     1081 attttgattt attagtggca ccggaacaaa atcagacact agaagtaatt atcagcaatt
     1141 cttcagatga agaacgaaca tttgaggtct ctgtcaatcc ggcggtaacg agtgatggtg
     1201 gaacgatcga ttatagtcaa aaaaacccaa cgttagatga gacattgccg tttgatgtcc
     1261 gtgacgtttt gctcattgcg aaaaaggaaa tcaatgtatc agcacatgca gaaacaactg
     1321 tacctataga agtgaaaatc ccagcaaaaa gctttaaggg acgtgtatta gcgggtattc
     1381 atgtttcacc taaagaagaa gctgaaacag aaaatgctac agaaggagca caaatcaaaa
     1441 atcgtattgc ttataatctc gcagtcgttt tacaagaaag tcaagagacg atagaaccgg
     1501 atctgaaact tttatcagga gatttggacg aggtcaacgc aaagccaact gttcagcttc
     1561 gtttccaaaa tcctcagcct aggattatca gcaatttgat atttacgagc aagatctttt
     1621 atgaaaatca gctttatatt gaaaatacgt ctaatgcatt tttagttgca ccaaattcga
     1681 attttcactt gaatcttgat ttagctggtg ataaagcaaa agccggcgat taccgcgcag
     1741 aaatcatagc aaaatcaggc gacagtaatg aatggcgatt tacacaaaac tttacgatca
     1801 aaaaagaaaa agcacaaaaa gtaaatgaaa attccgtttt tgcagtacaa gaacaatctt
     1861 tcccatggct ttatgcagga ttggttgctg cagttctagt gttatggttg attttgctgt
     1921 ttttgtgggt ccacaagaaa aagaaaaaag gagaaacaac atgatgaggt gatttcgttg
     1981 aaaacatcga tcttattggg agggattatt gcaagtttgc tgttcttcag ctggcaagag
     2041 ccagtccaag cagataccaa aagttcaggt acgattacat tttatgatct aagtccaatt
     2101 ccgcatccaa gtggttcagt caatggcgca tcaaaacagc agacacagct accgatttat
     2161 aaagaatcta atcaacatgg cgtattcccg gagacgaatg aacaaaagat ccgttttctt
     2221 tatttgatca ttggtataga attatggctt cttgcgtggt tgttgttatg gagaagaaag
     2281 gaaaaggagg atgaacatgt tgctacaaaa taaattttca ttttttcgct ccttcttttt
     2341 tacttcgctt tttgttttaa gtatatttat cttttcacct tcggcagaag ctgttggagc
     2401 aacgcctcct gccatttctt atgatccata cacgtatgaa aatatcgtac ctccaaatgg
     2461 tggacgtagt gcagtcaatg gcgggaagct tgtccaacta actggggcta accaattgcc
     2521 taaattgact gctacttatt tgaacgatgg ttcaaatgat ccttggaaag gtggcacggt
     2581 ccgttttcat tgggaaagcc atgtgaaact aacaaatgta cttgatttgg tgaatatcac
     2641 tgcacaagga tttgcatttg tggcgatcat caatttgccg gaacatgtat caagcagtga
     2701 tgtattaaaa gcaattgact ggaacaacgc ctacttgtat attgccggtg cccccatcaa
     2761 ggttactagc ggaagtttat tatcagcagg aagccatacc ttgcgttttg ggatggggtc
     2821 gtgggataca aatactctat tgacagatat tattgctatt attacgcgag gcgaattcaa
     2881 cgtggacgat atcccgcttc aatttgatgt tacagctaat gtcgcgaaga tgacagaaaa
     2941 cggagataaa tatgatacga gtccaaacaa aattttgacc aagggtaaat tacaaccttc
     3001 tatgtctaaa aaggccgttt tttctgttga tttttatgat tcgaataata ttatccaaga
     3061 tcgtatgttc aattcgattt tagctccagc tgcaggtaga ttttatccta aattaactgg
     3121 cggaaacttg aatccaggag catatgcatt ttctgcaagt gcagagataa atacgtggaa
     3181 tagttacatc tcaccaagtg attcaacagg aaaatataat acatttgctg ataccacaga
     3241 atacgtggat ggaaataacc agaaccttcc aggctctacc aatagtcgga cattgacgat
     3301 ggggctgact gaaggacgtt ttgatcagtt gccatttagt cgctttgatc gcgtagtcaa
     3361 ctattttaca ggaaaaaacg ttacacaagg agcaatactg actcatactc cagcaagcac
     3421 aagtggattg aatcagacag ccaatatcgt gtacagtggg aaagatgcgc aaggcggatt
     3481 gctttctcct gttgctttaa aggtattgga gcagtacaag ctagacggaa cggttttacc
     3541 aacaaactta acagccgata gtcagtgggg aaagctcctt tcttctgcat cgtaccaagt
     3601 cacggccaat tggtcagcaa ctgaactaaa aaagggttcg atccattaca ggctttattc
     3661 gaaaggaacc caaaaattag cgacaggatg gacagatcaa cttttccaaa cgatccaatc
     3721 aaataacggg ggtgtaaata cagctacttc tacactacct gcattggctt cagggcaata
     3781 ttattttgat tatcgcttgg tggacgatgt acttactaac atgtatccgt cttttgcgta
     3841 caaatggcag tctgaacaag ctggaatcac cactcttcct caaataacag tggctaattt
     3901 tccatctgtg agtgcagata gtcagctaaa aaaccttagc agacaacctg agacaggaga
     3961 tcccctgtct gctctatcag gagatacgat tcaagaaaat gtgacattca cgttaacaaa
     4021 atctggggat gcgataaccg acaaaaaaat cacgatctca cttcctgaaa atactactta
     4081 tatcaatggt tccttaaaat taaatggtac aactatcgca gataccggca ttcaacaagg
     4141 agtttctgtc ccagcagatc tattgagcaa aataggagat accattcatc ttacttacaa
     4201 ctatcaatta aatactgtag atacttcttt acaatccgtc agcatactga caaaggcagc
     4261 tgtactttcc agcaatatta cattagcaga tggtgctaag cttcctaacc cagttgtcca
     4321 aacatctgct aaaacaattc ttgtccccaa acaagaatta acattagtta atgtaccaga
     4381 tgattttaca tttggtaacg atctccccaa accgttgaag acaagttatt atgaatcaaa
     4441 aggcgatttt tcttttgatg tgcgggatac tcggcttcca agcacgtctc cttggcaact
     4501 gacaggtaca ttgacttctt tatttaagaa tgatcaaggt caagaattaa gcggaacaaa
     4561 attatacttc aatcattctg gatcaaagca attgattcag caaggtcaaa acacgttgat
     4621 ttatgagagt gatggaacag ctaaaggaga ggttctcgtt gactttcccg ataccgatgg
     4681 tttactctta gaggtaaata gcagtacgaa tgctcaacct ggcgcaacgt atcaaggcat
     4741 ggtcacatgg gaactgacag ctgggccaac cagctaagtg attagaattt gtatggatct
     4801 gtgataactg tcacagatcc tctttttcaa ggatgagaag gaaacttctg ctgttaaaat
     4861 cagaaatgct tgaaggagtg gacttggaac ttttcagttt gcttgaaaaa aatgaacaac
     4921 ggcaaatcaa acttttccaa cttttattag aggaaaaagg agaggtttcc gttaataacg
     4981 tacttacccg cttatcagtt gatcgggcta ccttgaaaga agatctgttt tatttacaag
     5041 ctaacttgga aaatatcaaa caattggaaa ttgttatcca agaagggaat attttattaa
     5101 tcagacatgg aaatatagca gcagtcgatg tttattatca ttgttacttg cgctattcta
     5161 caaaatatca gatacttctt tatctgttgg aaaatggctc atttgatcgg cagaaacttg
     5221 tcatggaact taatatcagt tcggctactt tgtctcgcag agtgaaggaa ttaaatgatt
     5281 ctttaaatga gtttcatctg aaaataaaaa gcgggcggct gaccggtcca gaaagtcaaa
     5341 ttcgttattt ttatttccaa ttattttggt ttggacgtcc ctatcttgaa aacaaaaaag
     5401 aatacagtga tttatcagga aacacttttt tagatctgct gcaaaaagag tttagttttg
     5461 ctattacaga agaaggacga ttgaaatttg aaatatggct ttccattatg aagaagcgaa
     5521 tgcgctgtac gaaaaaatcc aaaccagatt atagcaatct tcctgttgga ttgtttcaaa
     5581 cgacacaatt tttattggac ctccatacat ttttgacccg ttacttcttt caatatgcct
     5641 ttccttggaa tcaaaatgaa acgttggttt tttatctttt catgatcagt aactttacat
     5701 tagatacaca taactcgcat gtccaggagt ttttgataag cgaaaaatac aaagacgacg
     5761 tagtagctca attgaatcga acgtttaaac aatcgataga agaatatttc tctttttatg
     5821 tttttccaga agagatttcg caaaagattg cgttgacagt gatgcaaaat cacgctcatt
     5881 ttgcctattt tagaggatgg atgaatattt ttggccaaga aggattaaaa cagcggttaa
     5941 ctgaagtaga agataaacaa ttcctggttt tatgtgaaag attggtcaga caaacatttg
     6001 ctattgttga ttttactgaa gtagacgagg aaaatgcccg ctttgaatta tttgctcgct
     6061 atgcatctat tttacggacg ctgatcaagc agttagaatt acgtctgcaa attgcttatg
     6121 atcttcctta tgaaaggcta atggcggata tcgtgaaaga aatcatccag gaacaattgc
     6181 tgaatacgat caaatatgat ttagtcgaat acgagaaaga aaagcagtat gatatcattt
     6241 tgactagcca attgaaagag tacccgtcac agaagtccgc taaagttttt gtattcacta
     6301 gcaacgaata taattatgat ttcccctatt tgaaccaatt tttaaaagaa tgttatttag
     6361 aaaaattgaa tttaaggatg aataaaacat gaaaatcgtg atcattggtg cttcccatga
     6421 aggcatcaca gctgccctaa atctaagaaa acttcaaccg aaagcagaag ttttactgat
     6481 cgatgaagat cataaagatg gactaggcta tgtttcaaat ggaatcaacc tttatttaaa
     6541 aggaaagata cgtagtttag ctgaagttgc ccataacatg cggactcttc aagaaagtgg
     6601 ggcaaaactg atcaccgaat ggagagtaac tgaacttgat ccagacaaac atcagctcat
     6661 cctcgcatcc aaagaaggaa aaaacgagac cataacctat gataaattaa tcgtagcgac
     6721 aggttcctct cctgtcacct tgtataaaca aattgaagct gaaaatgtct acacctataa
     6781 aaatctagtg caatctaagc aagtacttgc cgctttaaaa gaagcaaaag aagtagtgat
     6841 cttcggtgct ggttacattg gattggaatt agcagatgct ttgagaaata aaggatacat
     6901 tattcatcta gttgattata tgccgaatgt tttatcaaga tatttcgata aggatatgat
     6961 caacagtttt caaaatcagc tgcaaactaa gcaaataaat ttttatccaa atgaattctt
     7021 gatcgattgg aaaaaatcag aagagaaagt agtatccgta caacttctgt ctcaaacaat
     7081 aaaggcagat atggtcattt tttctgccca aactcgtcct aatacgacat tgttaaaaga
     7141 aaaagtggct ttgtatgagg atgagactgt gatagtcaat gaatatctgc agacaagtga
     7201 tccggacatc tatgcgattg gagatatcgt cccagtcagt tttgacaaaa ataaacggca
     7261 tctcttttta cctttagtta ctcgagctgt acatatggca agagcagtag cgctaacttt
     7321 gagtggtcag ccaaccgctt acgacctcag acaaaagata acagcagcag tcattataga
     7381 ctacttttta ggaactgtag ggctgacaga agatgaagcg ccattcttgg aacaaaatac
     7441 cggttcgtgt tcaggagaat ttgatctatt tccacaatac gacgaagaaa acaaaacagt
     7501 aaatgcaaag ctgatctacc atccggatac attagaaata attggcggac agctgataag
     7561 ccaagaattc ttattatcag atttgaacct gcttgcggat atcgtgaaac acaaaactac
     7621 gattccacag ctagcagttg aggattttgg atttctgtct gaatacacct ctcgttttca
     7681 ctacctcaat gagttagctt ttaaagtact agctaagaga gcaaatagca atagagtaga
     7741 taaaagactg tgagaaatat cacggtcttt tattgtgtgg ggatataata aatattaatc
     7801 aatcgagaat aaaaaattca tataatgaaa tagcggaaga attttttgag cataatattc
     7861 gccaaaaatt tatcaagtga taaaattgtt gtataaaatg attatttcag gaggaggagt
     7921 taaatgtttt tagcattaaa tgaaattatg cattcaaagt tacgttatgc gttagttgca
     7981 ggggtgatgt ttttgatcgc ctacctggta ttttttttga ctgggttagc ttatggctta
     8041 gctcaagata atcgaacagc agtcgacaag tgggaggcag attcgattgt gctttcgaaa
     8101 gatgcaaatt caaatctagg gatgtccatg attactaaga agatagcaga agaagtcgaa
     8161 ggtgggaaag tggcgtatct tgcccaaaca cctggagtcg tgactagtaa agacagtaca
     8221 gaagaaggaa aaatcaatgt tagcttcttc ggtatcgata aaaatcaatt catcatgccc
     8281 aatcttgttg aaggaaaagc ttttgataac gatgatgagg cagtaggcga tattagtttg
     8341 aaagaagaat atggtctagc cataggagat acagttaagt tatctggtag tgacaagacg
     8401 tttaaactaa ctggctttac ggatcatgcc aaattcaatg tctcaccagt tctctataca
     8461 accatcaatg cttaccaaga aatccgcttc gaaaaagaag atacaagtga aaatgcacgg
     8521 attaacgcaa tagttgtcag aggtaaaatc agtaatttac ctgaagattt agaacagatc
     8581 aaaatttcta aattcatcaa tgaattacct ggatataatg cgcaagtatt gacttttgga
     8641 tttatgatcg gatttttgat cgttattgcg gctattgtga tcgggatttt catttatgta
     8701 ctgacgatgc aaaagattaa tatctttggt gtaatgaaag cacaagggat cacaggagga
     8761 tttattgcgc gttctgtagt ggcacaaaca ttcattttat catttgttgg tattcttctt
     8821 ggtctattag gaacagtggg aacatccctt gttctacctg atgcggttcc attccaaagc
     8881 aattggctgt tttttggcgt cattagcttg ttgatgttag tagtagctgt gttaggcgca
     8941 ctattttctg ttcggacaat cgtaaaaatc gatccgttga aggcaattgg ataagaagga
     9001 ggaagaacat gaaagcaata gaatttaaag aagtagaaaa aagtttccaa gatggcgacc
     9061 aagtagttaa agcattgaaa gaaacaacca tgagtatcga taaaggggaa tttgttgcaa
     9121 tcatcggtcc ttctggttca ggtaaaagta cttttttgac tttggcaggc ggattgcaga
     9181 cgcctagcag tggctctgta tttatcaatg atcaagaatt cagtaatcaa aaagaaagcg
     9241 aacgcgttaa actacgtttt aaagaaattg gctttatttt gcaagcttcc aatctggttc
     9301 cttttctgac agtaaaaaag cagttattat tagtagataa gattaaacat gaacatcgtc
     9361 aaaaagaagc aaacgaatta tttgagcagc ttggcgtgga taagttgatc aacaagtatc
     9421 cagaagaact atctggtgga gaacggcaaa gggtcgcaat cgctcgtgca ttgtacaatg
     9481 atccgtccat tattctagca gatgaaccaa ctgctagttt ggattcagaa aaggcacgag
     9541 aagtagtcga tatcttggca aaagaatcaa aagacaaaaa taaagcaacg atcatggtta
     9601 ctcacgatac acggctgatt gacaaatgcg atagaatatt tatgatggaa gatggtgtat
     9661 tgaaagaaaa agaataacca aaaagcaggt ggaaaaatgg tccatcgata caacaaagat
     9721 acacagcaaa aaattttgaa agaagcgact gacttgttta tgtccaaggg atatctcggc
     9781 acttcaacaa gagaaatcgc acaaaaagtt gggatcaccc agcccaatct ctatcattat
     9841 ttcggtgata aagaaaaact atatacggca gttttagaaa accacttaaa agatgttgga
     9901 aaagctttga gagaaatcgt tcaaacaagc gaaacgggat ttcaagtaac cttgacaaaa
     9961 atggcgcagt ttttgattga gacacatctg gtcgatttgt ttctgatgct tcacgatctt
    10021 gaaagcaatc tttcaaagga aacaagagat catcttttct ttctatggaa gaaaaattat
    10081 cgtgaacctt ttgaagaaat cttttcagca aaccaatctg tgctaagaaa tgggatttca
    10141 aaagaaatcg cagcaaggca ttttttctta gtgttagcac cttacatcac tcggtcgaaa
    10201 agacctgtgg aagaaccgct aaatgtggcg caattgatcg atttatattt gaatggtgta
    10261 ttaggagaag cgtaaagctg ttatataggg agaaaatagt taggtgaaga agcaagatat
    10321 gacgaagatc gttatatctt gcttctttat ataggtagtt tttcataaat acaaatttgt
    10381 tattctctca tgattatcag ggtatatttt tatttattat gtcggtgttc caggaagtgt
    10441 tttaatttaa taacgaagaa attaattgtt tgtagaatga aggaaactat ttattgggta
    10501 gatgaatcac cacgaatagt caaaaggaga tttcaggata tgccaaaaat gaaaaaaatc
    10561 attttagcag taatgttatt ttctacggtt atatctttta cagccccttt tttgcttact
    10621 taagctgatt ttaagttttg gcccttagtc tggatctacg aggtgagagt caagaatgaa
    10681 aaaaataatt ggagcttttg tatttgcaat cattatcatt attatctgtt ttcaaataga
    10741 tgtttttggt caaaaaaaga tagttggaca aacggtcagc agtacgaaca gtacggttac
    10801 aaagacaaag gaatcatttg atgatacaga acaagagaca ttaatcaaaa tcggaaaaaa
    10861 tgaccgtaca ctatatacag atgactcgct aactacccca cttgcaacta taaacggcgg
    10921 agagctggcg gagtttcttt ctgaaacaaa aaatgcttat cagatcaaga caaatgatgg
    10981 ctatactgga tatttagccc tcgaagatgg aaccaaagtc acaaagaata tacaaacaaa
    11041 accggaagag ctatctgatg cagtgattgt attagatcct gggcatggcg gaaatgatac
    11101 tggggcatta agcaatgatg aacaaacaga agaaaaagat ataacattaa gtacagccat
    11161 aaaagtaaaa aaggcgctag aggatgctgg agcaacggtt tatttgaccc atactacaga
    11221 tgaattagta cagcttggag atatatgcga ctacagtgaa gaaaaaaagg cagatgtctt
    11281 tatcagttta cacgcagact ctacggaata tgcgaatgaa gcaactggaa tcacgacata
    11341 ttattactat ggacaagagg aaacattagc acagacaatt gctgactcct tcactgatct
    11401 gcctctggat tcaagaggaa tctccactgg gaactatcaa gttcttcgag aaaatcttca
    11461 accttctatt ttaatcgaga tgggttatat gaataatgat agtgatttaa aggaattagt
    11521 aacagacagt tatcagcagc agattgctgc aagtttgaca caagcattga ccacttattt
    11581 tcagtaataa ataagcccag ccaaacaaaa atagctcctc atatttttcg tttggccggg
    11641 cttattatcg cagatgcaag cttttaaaca ccgtttaagg aagaggtcgt gagcctgccg
    11701 ttcaactctg agtcacaagg agcaatttca aaaaacagct tctcatgttt gatgactatt
    11761 gtgacttgtg accgaggagt tggcatgaga acaccgttta agaaaaaggt tgtgaaatca
    11821 acgttttgtt ctgagtatta gagaaacaaa cggaaaaata gttcctcata ttttgccgtt
    11881 tgttgagcta ataccgagga tcagatgatt gaacaccgtt tattcggaac aagggactgt
    11941 gacatgactt ttgtcacagt tccattattt ttttagtagc ttcaaagctt tttctgtatt
    12001 ggcaaaatga agtttggcat atttttcaag cataggcatg ccgcctttct ccaatagttt
    12061 tgccgctact tggtcagatt tcgttccagc tcctgacgga agggtgattc cagcttcagc
    12121 tgatgctttg tctaactctt ccaaaaagct ggcacgactg atgatagaag cagcagcaac
    12181 agctaaatga tattgttctc ctttagtaac gaagtacagt ttttcttgta attgattttt
    12241 ttctaaacgc gcatattttt taaaatttgc ttccggcgta aactgatcga tcaaaatcgc
    12301 ttctggtttt tctggctcga ttttttttag cagcaaatgg atcgcttgat tgtgcaaagc
    12361 gactttcatc cgaacggcat tgtattttgg ttggatctca ttgtattttt ctggagataa
    12421 gacaagcagt ttatagggga tggttttttt taagatgtca gcaagctgga tgatttggct
    12481 atcttttaac tcttttgaat ctctaacacc aagtttacgt aattctgcaa gctgatcacg
    12541 tttcacataa gcagcacata ctgttagcgg accgaaatag ctgccatttc cgacctcatc
    12601 actacctaaa acggataatt gactgatatt ttttggcaaa gaagaggctg tttttgctgg
    12661 cttggcttgt ttatcctgtg cctcccccca tttatcagct tctattgctg cattcgtacc
    12721 ttgaaacatc acttttccgg atgtataagc ggtaatggtc gtcccgtctt tttttgcagc
    12781 aaagctagta taaggaatct gtttatttaa caaataagag cgatagtatg ctttcatttg
    12841 actaagttgg tcgtttgttg ctttgataat gacgttgctc ataaaattct cctcgttttt
    12901 atacataaaa ctataccgca actcacgact tttcgttttc taagagattg cgagacatag
    12961 cattttttta aagtttatca tataatagtt aagtatgtga aaaatatttg tggagaacgg
    13021 tgaaagaacc aacttcatgg tacactaaat aaaactttca tcaaatagga ggaaatgatt
    13081 cgtatggcgc acgaaaaaac acgctacaaa gcggtgattg ccaaccaaac atatactatt
    13141 atcggacggg aaaccaagca tcatatggat atcgtaacga agctgatcaa tgaacagtta
    13201 gcagaattga aacaattatc accacaaatg gataatgaac aagcagcaat cttgatggct
    13261 gtcaatgcgc tttcggatca attgaagaaa caagaaagaa tattggagct ggaagaagaa
    13321 acagcagaat taaagaaaaa aatgatcaaa tttacagagc ttgaaaatcg tgtgaaacgc
    13381 attgaagcaa tcgagaacga agctcgggaa gtattgaaag aaaatggtca agcagatcat
    13441 accattaaaa atcatgtgga agcacaacag attttaaacg agaaacgaaa aggacaaatt
    13501 aaacaaaaat cttctaatta agatcctttt atcaaaataa gaaaaattta aaaaagttag
    13561 aagagaaagg aacgtagcat gctgagtttg ctgattttat ttttattgct aattgcgttt
    13621 ttttctggag cacgtcgcgg gtttgctttg caaatcatat tcgcgattgg atatgtattg
    13681 tcatttattg ctgcccagca tttttacaaa ccattagcaa gtcatttgga attatacatt
    13741 ccatacccag ctgtcacccc aacatcaaag ttagcatttt ttgatcaggt atttgccttt
    13801 cacctagatg aagcttttta tgcaggaact gcttttctga ttattttgct aattggctgg
    13861 ctgcttaccc gatttgtggg ggtttttgtc catggattga cgtatgtgcc gatcttgaga
    13921 caggtagact ggattgcagg aggcatcctg tcactaatca tggcttacgt tactatattt
    13981 ttgattttgc agttacttgc ctttgttcca ttagatgtgg tccaaaatca attcggcgag
    14041 catagtttcg ctcgttttat cgttgagaga acgccatttc ttaccaacaa gatgtatgag
    14101 ctttgggtaa cgaatatttt gaattaagga aaatagtgct ggaaaatatt tcgttttttg
    14161 tacagagtag tttatttaag aaggctgcgt agcgtatgtc cggcacagct cttttttcat
    14221 agtgaaagat aaggtgataa aatgaataac cgaatccttg aaacattgga atttgaaaaa
    14281 gtcaaacaga tggtcagaca atttgtagtg actgcacaag gaaaagaaga actggcagaa
    14341 ttggtaccag tgagtgagaa acaaacgatc acaaactggc ttcaagaaac tgaggatgga
    14401 ttgaaagtgc aacgtctgcg aggcgggatc cctataccta aaattgaaaa tatccgtccc
    14461 catatgaaac ggattgagat cggagcagac ttgaatggag tggagcttgc acaagtttcc
    14521 agagtattgt ctacgacaag cgaattgaat cgtttcattg atgatctttc tgacagtgaa
    14581 atcgaatttg cacgtttgta tatgtgggca gatcagttag tcacgatccc tgttttgagc
    14641 cgccgtttaa aggaagcaat cgatgaagac ggacgcgtga ctgatgacgc ttctcctgag
    14701 ttaaagagca taagacagaa cattcggcga agtgaacaag ctgttcgtga acagttagat
    14761 gggatcgtcc gtgggaaaaa tgcaaagtat ttaagtgatg cgatcatcac catgcgaaat
    14821 gaccgctacg ttatcccagt caaacaagaa taccgcggtg tatttggcgg tgtagtccac
    14881 gatcaaagtg cttcaggtca gactttattt attgaaccaa aacaagtggt ggatttgaat
    14941 aatcgtttgc gtcaatacca aatcgctgaa cgaaatgaga ttcaacgtat tttaagtgaa
    15001 ctttctgctg aacttgttcc ccatcgtcaa gaaatcatcc acaatactta tgtgattggt
    15061 aaaatggatc tgatgaatgc aaaagcacga tttggtaaag aagtaaaagc catcgtgccg
    15121 ggaatcagtg aagacaatca tgtcgtcttg aaacaagcaa gacatccact gatcgatcag
    15181 gaaaaagtag tttctaatga catcacaatc ggaaaagact atcaagccat cgtcattaca
    15241 ggacctaaca caggtgggaa aacgatcacg ttgaagactt taggtctgtt acaactgatg
    15301 gggcaagcag gattaccgat tcctgcaggt gaagaaagtc aaatcggtat ttttgaggaa
    15361 gtatttgcgg atataggaga cgaacagtcg attgagcaaa gtctatcgac cttttcttct
    15421 catatgacga acactgtaga tattttatca aaagtcaatg aaaaaagtct ggtattattt
    15481 gacgagctcg gtgcaggaac agatccgcaa gaaggtgctg ctctagcaat cgcaatctta
    15541 gatgatcttg gtaaaaagtc tgcttatgtg atggcaacaa cgcattatcc tgaattgaaa
    15601 gtttatggat ataatcgggc aaatacgatt aatgccagca tggagtttga tgtggataca
    15661 ttaagtccaa cgtatcgttt gctgattggc gttcctggac gaagcaatgc gtttgaaatc
    15721 tctagccgat taggtcttga tacagaagtt attgatgaag cgaagcagtt gatgaatgat
    15781 gagagccaag atttaaacga aatgatcaca gatttagaga atcgccggaa aatggcagag
    15841 accgaatatc tggaaatgcg tcattttgtt tctgaggcac aagaacttca cgatgatttg
    15901 aaagaagctt acagctattt ctttgaagaa cgtgaaaaag aaatggaaaa agcaaagaaa
    15961 aaagcaaatg aagtcgtgtc agaggcggaa gaaaaagcag aaaaaatcat tgctgatatt
    16021 cgtaaaatgc aacagcagat tgggcaagga aatgtgaaag agcatcagtt gatcgatgca
    16081 aagacccaat tagcaaatct acatcaagaa gaaactttga agaaaaataa agtattgaag
    16141 aaggcgaaag aacaaaaaac gttgaaaccg ggagacgaag tgttggttac gacttacggc
    16201 caaagaggga cgcttttacg taaaaacggg aatcagtggc aagtagaaat cggtatatta
    16261 aaaatgaatg tgtccgaaga cgagctgacg cctgttgctc ctcaaaaaga gccgacacaa
    16321 cgagttatcc atgctgtacg atctgaatct agtagtcacg tgccgaatca attggacctt
    16381 cgaggaaaac gatacgaaga agcgctaagt gaggtagatc aatatttgga ttctgccatt
    16441 ctagctggtt atcctcaagt gactattgtc cacggaaaag gaacaggcgc acttcgcaag
    16501 ggaattactg attatcttaa aaaccatcgg agcgtcaaaa gctttgagtt tgcaccggca
    16561 aaccaaggcg gaaatggtgc gacaattgtt aaatttaagt aaagcttact aaaggtaagc
    16621 agatggttcg ctatccaagt atagaaatgc tatactagtc atagatttta aaaatggagg
    16681 tctttgatat gtcacaagta attacagata agacatttaa tgaagaaaca gataaaggac
    16741 ttgtattgat tgatttttgg gcaacttggt gtggtccatg tcgtatgcaa gcaccgattt
    16801 tggatcagtt ggaacaagaa tatgatgaag aagaattcag aattgctaaa atggatgtgg
    16861 atgaaaatcc agaaactcct caacaattcg gtatcatgag cattccaaca ttaatgttga
    16921 aaaaagatgg tcaagtagta gaaaaagctg ttggtgttca ttcaaaagaa caactacgtc
    16981 aaatgatcga tcaatattta taaaaactta cagcaaaaat aagctgaggg tgggactgaa
    17041 tccagttccg tcctcggttt ttttgcggtt gagttaaggt tattcaaatt aatatttttt
    17101 gagtataatt acgttgtcag gctggattgt ttttttgaca gacatgctat aataacttta
    17161 attgaatatg aaggggagta ccagatgaac gaacgaatca aaaacaaatt agctttactt
    17221 cctgatcagc ccggctgtta cttgatgaaa gataaaaatg gaacgatcat ttatgtcggc
    17281 aaagccaaaa tattaaaaaa tcgtgtccgc tcatatttta ctggtagcca taatacgaaa
    17341 acggaacggc ttgttagtga aatcgaagac tttgaataca tcgttacaga atcgaatatc
    17401 gaagcattgc tcttagagat caatctgata aagaaaaatg atcctaaata caacattatg
    17461 ttaaaggatg ataaaaccta tcctttcctt aagataacga atgaaaaata tccccgtttg
    17521 gtgattactc gtaaggtact aaaagacaaa gcactgtatt ttggaccata tcccgatgtc
    17581 ggtgcggcga atgagacaaa gaagatcctc gatcgattat ttcctctgag aaaatgcaaa
    17641 cctagtcaga cgaaggagcc ttgtctttac tatcatttgg gacaatgcct gtgtccttat
    17701 tatttcgacg tagatccgaa agtctatacg aaaatcgttg atgaagtgaa aaacttttta
    17761 aacggaggac acgaaaaaat cgagtcagag atccatcaga aaatggaaaa agctgctgaa
    17821 aacatggagt ttgaaaaagc tgcagaatac cgagaccaga tccgtgccat tgagacaatc
    17881 atgactcgcc aaaaaatgac aaatactgat ttactggatc gagatgtatt tggttatgcg
    17941 attgataaag gatggatgtg tgtccaagtg tttttcgtaa gacaaggaaa gctaattgaa
    18001 cgagatgttt caatgtttcc tttctataac gaagcagaag aagatttctt gacttacctc
    18061 ggtcagtttt atcaggaaaa tgagcatttc attcctaaag aagtcttgat tcctgatacg
    18121 atcgacaagc ccagtgtaga agctttgtta gctacgaaag tgctgcaacc aaaacgagga
    18181 gaaaagaaaa aattagttga attggcgagt aagaatgcct cagttgcttt gaatgaacgg
    18241 tttgatctga ttgccagaaa acaggaacgg acgatcggag cagttgaacg tttaggaaac
    18301 gcgatgaata tcccgacccc aatacgtatc gagtcttttg ataactctaa tatcatgggg
    18361 acgaatcctg tggctgcgat ggtcgtattc attgatggaa aaccggcaag aaaagaatat
    18421 cggaaatata aaataaaaac agtcaacgga ccggatgatt atgcatcaat gagagaagtc
    18481 atctatcgca gatattcgcg agtgattcgc gaagggttgc ctttacctga tttgatcttg
    18541 atcgatggag gaaaaggaca agtagatgtg gcaaaagatg tcttagaaaa tcaattagga
    18601 atcgatattc ctattgctgg catggtaaaa aacgataaac ataaaacaaa tgagttgcta
    18661 ttcggtacag atttggctgt agtaccgctt gagcgaaatt caccagagtt tttcttactc
    18721 cagcgaatcc aagatgaagt ccatcgattc gcaatcactt tccatcggca gctacgcagt
    18781 aaaaacagtt ttgcttcccg attagatgaa attgaagggt taggaccaaa acgaaaaaaa
    18841 atgctgctta aagagttcaa atcgttgaaa aatatcacag cagccacgac agaagaatta
    18901 caagaaatcg gactgccgaa aaatgtcgca caaaacgtat ttgataaatt acatcaggaa
    18961 tgaggaaagg gcactttaca aagaaagtaa agtgtcctat cattttatgt acaacaaaaa
    19021 gagcgtttac gttttgttgg taaataataa atcgcttacg tctggtgtct aaggatatat
    19081 gtgatttctt gaagaaggag tgaagaaatg ttagaagtca aggatcttgt taaaactttt
    19141 gggaactaca cagctgtcga tcatgtctct ttccaaatcc ccgatggaaa aatcatgggg
    19201 ctaattggcc aaaacggggc tgggaagaca acgacttttc gtttgatctt ggattttttg
    19261 acacaagacc aaggagaagt gttatggaat ggccatcatc ttggggagaa agattatgat
    19321 gtcatcggtt atcttccgga ggaacgggga ctttatccga aagtaacgat tcaagaccaa
    19381 ctgctttatt ttgcagaact tagagggaaa tcaagaaaag agattgaacc aaaaatcgac
    19441 ttttggatgg aaaaatttca ggtaaaaggg aaaaagacgg ataaagtaaa atctttgtct
    19501 aaaggaaatc agcaaaaagt ccagctgatc gccacgttaa tccatgaacc taaattgatc
    19561 attttggatg agccattcag cgggttagat ccggtaaatg ctgaattgct aaaagatgga
    19621 atattggaat tgaaaaaaca tggttcatgt gtcatttttt ctagccacaa tatggataat
    19681 gtagaaaaaa tctgcgatca tttgatcatg ttgcgaaacg gaaaaatggt tttgaatgga
    19741 aaagtacatg agattcgtga atcatttggc cgtacgaagt tattcttgga atctggttta
    19801 agccaacagg aagtagaaga aatcgaaggc gtgaaaaaag tgatccttca tgaagacaat
    19861 acgttagaga ttaccttaga agatcctgaa gctgggaaag aaatctttac tcgtgcgact
    19921 cagttcggct acattcctat gtttaaccag caaccgccaa ctttggaaga gattttcaaa
    19981 ttgaaagcag gtgaaatcga tgcgtaagtt ttggattatt accaaagatg tctatttaaa
    20041 aaatgttaag tcgatttctt tcttgatcat gatattagtc ccttttatca tgctgggtat
    20101 catttatgta gcaggaaact ttgctcaaca aaatagtgat gtcaataaaa tcggcgtatt
    20161 ggctgataat gagcaaatca gtcagcaaat gagccaagtc aaaacagatg attttgagtt
    20221 caaagcagtt gattctgaag aaaaagcaca aaaagagctt tcggaagaaa aaatcgatgc
    20281 gtatttagta gtctcaactg agaatgataa gataaatggg atgctttata gcgaaaattc
    20341 attaggacaa agtacacaac tactgataca acaacaactg actggcttgc aatcgatgct
    20401 tcgagcgagt caattaggca tttcaccaga agaagtagct agcttgagcc aacaagcaag
    20461 tttgactcga caaaaagtca attttgattc aaaaggaaaa atgacagtcg gagaagataa
    20521 ctcgacagtc caatacgtcg taagttatgt agcaacgatt gtcttgttca ttattatttt
    20581 gacgtatgcg cagatcattg cacaagagat cgcttctgaa aaaggcacgc ggatcatgga
    20641 agtgatcctg tccagtacga ctgcacaaaa gcatttttac ggaaaattaa caggtgtttt
    20701 acttgttgca ttaacgcaaa tggcattata cggtattatt tttggtatag gatacagtca
    20761 attcaaagat atggaaatgg tcaaaaatat attagacggt atctcattgg ataacttgat
    20821 tggaccattc ttgtggtatt ctcttctatt catgatcttt ggcattttga ttttttctgt
    20881 tttagcagct ttatgtggct ctttggtcaa caaagcagaa gatacagcaa aagcaatttt
    20941 gccagtgact tatctatctc taggcggata catgcttgga ttgatactag gagcttcaga
    21001 tccaaacaat atcgttattc ggatcacttc gtatattcca ttcttatctt catatattat
    21061 gccgattcgc ttagcaaatg agacagtcgg tatgggtgga gcattgattt ccttgttggt
    21121 acttgtagcc gtaacattct tattgatgat cttgtctgca aacatgtaca aatcgaatgt
    21181 actcgtttat agcgaaggcg gattatggag ttcattgaaa caatcgattt caattatgag
    21241 gaatgaacga aaaaaagcat aaaacaatcc taaaatacag aaggaagctg gacatctatt
    21301 gttcccagct tctttttgta tagacctatt ttatttattt tactatattg gatatatatt
    21361 cattgaaaat agtgtataac aactttataa tattgagtat accaattttt ggaggcgttt
    21421 taatggaccg ttatttagat ttactttcag aaaaattccc ctcaacggag gatgtcatca
    21481 cggaaatcat caatctcgaa gcaattttgc agcttccaaa aggaacagag ttatttttga
    21541 gtgatataca tggcgaattt cctgcttttg atcatattct gcgtatcggt tcaggaaatc
    21601 ttaaagaaaa gatcaaagac cttttttctg aacaaatgac ggaaacggat atcagccaat
    21661 tcactatttt tacggcttat ccagaatatg cattaacgac tgattggtac aaagaacaag
    21721 ataaatcgca gctgatccat catttgatcg atcttttacg gtttacgaca gtcaaatata
    21781 cccggtcaaa agttcgaaaa ggattgccga aagaatatag ctatatcatt gaggaactct
    21841 tgtatatcga tgaccgtatc aatggaaaaa aagactatgt aaaaaaaatc attgaacaac
    21901 ttgttttgat gaaggaagaa gaaaaatttt taacgaaact tgcacagacg atccaaaaat
    21961 ctgtcatcga tcatttgcat atcgttggag atatttttga ccgaggaact caagcagcca
    22021 aagtgatgga tcgaatcatg gatttttctt ctgtagatat ccaatgggga aatcatgata
    22081 ttttgtggat tggtgcgtat tgcggctctg aggcatgttt actgacactt ttacggattg
    22141 cagcacgcta taattattta tttgaattag agaaagaata tgggttgaat ttacgtccat
    22201 tgttttcttt tgctcatcaa acttatcaga agaatccagt atttgcgcct aaaaacagta
    22261 aaaatctatc cgaaagagag caagaagaat tagaaaaaat ccatcaggca ctttctatcc
    22321 ttcaattcaa atcagaacat cagttattgg atcgtcgacc tgaatttaaa atggacgatc
    22381 gtaaattgct ggaaaaaatc gactatgaag catcaacgat tgatttagaa ggtcagctct
    22441 atgagttgaa aggaacttgt tttcaaacga tccagccaga tgatccaaaa aaattcttgg
    22501 aagaggaaca aaaagtgatt gatagtttga tgtattcatt ccagcactct ttgagaatgc
    22561 aaaaacatat gacgtttttg atcgaaaaag ggggaatgta tctcacgaac aatcagcatg
    22621 tgctgtttca tggctgtata cctgtagacg agaagggaca gctcttagca tttgaattag
    22681 accaatctta tcgcggaaaa gaactgctgg gcttttttga aaaacagata actgaaagcg
    22741 caaaagatct gactgcaaaa gaagatttag caaccgattt gatttggtat gcgtggagtg
    22801 ggccattttc gcctttattc ggcaagagta aaatggcgac ttttgaacgt tattttctca
    22861 cagagcctca tacgcacgtc gagaaaagca atccttatta tcacttgcga gatgaagagt
    22921 ggttcagtga aaaaatttta gcagaattcg acacaaaaga agaagggtca gcaatcatta
    22981 atgggcacac gccagtaaaa gtaaaaaaag gggagtcacc gatcaaagca aatggaaaag
    23041 tatttgtcat tgacggaggt ttatccaaag cttatcaaaa aacgacagga attgccggtt
    23101 attcactatt atgcaattct tatggtttcc aaatcgtgac acattatccg tttttgccta
    23161 ttgaacaaca atttgcaaaa aaatccgacc aaacaaatgt gaaaaaagta atcgaacagc
    23221 aactaccacg caaactcaat cgtgatacaa caaaaggtat ggaactgcag cagcagatta
    23281 cccagctcaa aaggctgttg gactatcgaa aggacgattg agaagaattc cagtgaacat
    23341 tgttccatag actggagact gagacaaaag tcgtgtcgca gtctcttttt ccggataaac
    23401 ggtgttcttc tgccaactcc tcggtcacaa gtcacactat tcaacaaaca tgaaaagctg
    23461 ttttttgaaa ttgctccttg tgactcagag ttgaacggca ggctcacgac ctcttggtat
    23521 aaacggtgtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtgaaaa
    23581 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca caaccccctc
    23641 agaaagtcta ttaaaattca ttcacaaaga aatcgaataa gcgctgttca gatggatgta
    23701 cacggtgggt aagttccgga tgccattgaa caccgaggat tttatgcata tcatctttgc
    23761 tttgaaccgc ttcaacgagt ccatcgttag aaaaagcgat ggcttttaat aaaggtgaaa
    23821 gatctttgac tgcctgatga tggtaagaat tcacaaaata tggatcgggg agaaggttgc
    23881 ttaacaaact gtctgaaaca atcttgactt cgtgagtcgt aaattgcggg gcagtgggct
    23941 gttgttcatg ttttactgac cactttggat ataatgaaag atcttgataa agtgtacctt
    24001 ccaaaacgac attcaatagt tggagtcccc ggcagactgc aaaaattggt tttctttgtt
    24061 tcaatgcttc caagatcagt gcctgttcaa attggtctcg ttgaatattt gtttccgtta
    24121 gctttggatg aggatcttcc atgtaaagtt gtggactgac atcttgacca cctgcaagaa
    24181 taagtttgtc gatttgtcca atcagttgag gagctgaatc tggagacatg actggcagaa
    24241 taagaggcaa tccttgagca tctaaaactg catccacgaa tccttgcggt gtatagctca
    24301 cttgatttcc ttggaaagta tctgttgctc gaatcagttg atttccggca atgccgatga
    24361 ttggtttcat ttgtattccc cctaaagtag acttattgta acaaggattt aaaaaaaatt
    24421 aaactatgta agacttgccc ctatacttat ctgttcttgc tgtgttacta tctgtaaaga
    24481 gaaatttcgt gtgaaattca tgacgatagt tgaaaatatt gcttaatttg ttataataaa
    24541 aagtaagcag aaataaaaag gttgggcgta agcttcaagg atctagttct tcaaggggtg
    24601 agaaggacat tttattgaag gggcctgcct atactaagtt atctttagag gtacatactt
    24661 tcaatgaaaa aagaaagcat gtacctcttt tttaaatcga aaggaagtga agcaagtgtt
    24721 agaagctttg gaacaaatcc gaaaagcaga agaagaaaat gaaaagcgtg ctcaaacact
    24781 gcggcagcag ctgaagatct atgcaacaga aaaagagcag gaattggaaa cgatccaaac
    24841 aaactatcga caacaactaa aacagttggt ggaagaaaaa gaacaatttg aaatacaaga
    24901 attagaaaaa gaaaaaatga aattgaaagc agaagcagac aaggtgcaag caactttgaa
    24961 agatcaatat caaaagcatc agagtcaagc gatcggtgcg atcattgaaa gggtgaaaga
    25021 aacttatggc cgtcactaag atggaaaaag tgaccctcat ttcagataaa agaaatcagg
    25081 agaggatttt acaagcagta caaggaatcc aaaacgttga aatccgggat ctgtatcaaa
    25141 atagaacaaa caaccagtgg gtggagaagt attttccaga taccacgatt attgataaag
    25201 aagcaaaatt aagcgcttta ggtaatcggt tgaaagacat ccaagaagct atccaattca
    25261 tacaacacca cggcgatagc cgtcaaaaga aactccattt gaaacgtcaa gaactttcct
    25321 tacaagattt ggaagccaat tattccgaag aaaatttcat aaataaattg aaagaagtcc
    25381 ttgatttaaa agaaaagtgg gaacaactaa ctgaagaacg tgatcgttta gaagaagagg
    25441 aagaatggct tcttcattgg caacagctgg atattgctcc aagtatgtat gcaagtaaga
    25501 cgaccatttt tgagatgggg actgtgagtg cagcaaattt tgaaagtttc aaggaagaac
    25561 tgagtaagct tgatgacgtt tatatggaag aaatagatta tacgccaaaa caagtttatc
    25621 tttcgtatgt tgctttaaaa aaatcagttg ctgttataga agaaattgct gggaaatacg
    25681 ggttcaataa agaaacgtat ccttatgaac aaaatcctaa agaaaaactg caagagacca
    25741 agcagcaatt atccattgtt cacgagcagc aaaagaagat aacgacagcg ttaggtcagt
    25801 gcagtggcta tatccaagaa tttgaatggg tagaagaagt cacacttgcc gttgcagaac
    25861 gagaaaaaat aaaagaacgg tttatccatg cgacttatct gatcgttttg caaggttggg
    25921 tagatgcaga agaaaaacag aatttgctgc atgtgttaaa cgaaaccgta tcagaagaag
    25981 atatatacgt cagtttcgaa acaccaaact cagaagagat tcagcaggaa gtccctacta
    26041 aattgaaaaa ccatccgctt gtcgaacctt ttgaattgct gactgaaatg tacagtctgc
    26101 caaaatatga agaagtagat ccaacacctt ggatgacacc attttatctt gtcttcttcg
    26161 ggatgatggt cgcagatgtt ggttatggtt tgctgatgct cattgggaca atactagctc
    26221 aaaaactact tgttctacca cgcgggatga aacgtttcgt aaaattcttt gagattctct
    26281 cgataccatc gatcatctgg ggattgatct acagttcatt tttcggacag tcattaccaa
    26341 aagaactgtt tggcatccgc ttgcctttcc caatcctatc tacgacggat gacgtcaata
    26401 cgattttgat tttatccgtg attttcggtc tgatacagat tttggttgga ttatttgtgg
    26461 cagcaaagga aaatatcagg agaaaagatt acttgagtgc tgtcagtgat ggatttgcct
    26521 ggcaagggat cctaattggg atcgttattg cgttggttgg tgccatgttg ataaaaaata
    26581 cagcatttgt ttaccttggc gctgggctat cgatcattgc tgccttatgt atcttgatcg
    26641 ttccgattat ccaaacgccg tctaaagtca aaggtgctgc caaaggtgcg tataatcttt
    26701 acggactgac cggctatatc ggtgatttag tcagttatac acgtttgatg gcattgggta
    26761 tttcaggcgg aagtattgga gcggcattca atatgctagt tgctttcatg ccgccagctg
    26821 ctcgattcag tgtagggatc cttttgatca tcgctttaca cgcactgaat atgtttttga
    26881 cactgctaag tgcctatgtc catggcgcac gattgcagta tgtcgaattt tttgggaagt
    26941 tttatacagg aggcggacgt gcctttgatc cgctgaaaac ggcggaaaaa tacgtcaaca
    27001 tcaatcacaa gaaaaaaaac aataaataat tggaggattt ttatataatg atggattact
    27061 taattagtca aaatggtgga atggtatttg cggtattagc gatggcaaca gcgacgattt
    27121 tttcagggat cggttcagct aaaggtgttg gaatgacagg tgaggcagca gcagcattga
    27181 caacaagtca acccgaaaaa ttcgggcagg ctctgattct gcaattgctt ccaggcacgc
    27241 aaggtttgta tggattcgtt attgcctttt tgatttttat caacttagga aatgacatgt
    27301 cagttgtaca aggactgaac tttctaggtg cttctctgcc gattgctttt acaggcttgt
    27361 tttcaggtat tgcccaagga aaagtcgcag cggcaggtat tcaaatttta gcgaaaaaac
    27421 ctgaacatgc tacaaaagga atcatttttg ctgcgatggt tgaaacatac gcgattttag
    27481 gtttcgttat ttccttccta ttagtgttga atgcctaagc ataaaggagg aaaagcagat
    27541 gaacgccatt gaaaaaatca tctctcagat gaacgaagca gcagagcagg aaagagcggc
    27601 gttagaacaa gaagagcgaa tgaagatcga tcaaaacttt gaacaaaaac gtacgcaagt
    27661 tgaaacagaa catcaaaaac aaaaagaaaa acaaatcgaa ttactcgaaa aaaaataccg
    27721 tcagctaagg aatcgccagc aagtagaagt acgacaagaa aatttgaatg cgaagcagga
    27781 atttttaaga cgattgtttg cagatgcggt aactgaaatg gaaaattggg atgaatctga
    27841 acaaatccag tttatcaaaa atgcattgta ttcattacct ttgacaggaa aagtagcatt
    27901 cattgctgga gaaaaatcag cagcctatct ttctcagacg ttgttggatg aatggaacaa
    27961 tgaacttccc tttatgatgg ttttaagtga tgaaactgtt gctgatcaag cgggttttct
    28021 gataaacgat caaggagttc agtataattt tcttttcagc agccttgtac aagatatcca
    28081 aggaacgatg agttttgaga ttgccaatca attattcgaa taaggaggga tcagtgtatg
    28141 gaataccacg aactaaatcc cttgattcgc ggaagagagc ttgaactttt gtccaatgag
    28201 acatttgaac ggatgattca aacaaattct gtcgaagcac ttggtgaaat attgaaatcc
    28261 acgatttaca gtccatatat ttatgacgga tatgaatatg acttcgaaca gaatctttta
    28321 aaggagcaga gccgtctatt tcgctggtta aaggaatcag caccggagcc tgaaatcgtt
    28381 tggatctata cgatgcgtta tactttccat aatctgaagg ttttgacaaa agctgagatg
    28441 acaaatcaaa atctggacca tttgtatatc gacgatggat tttattcatt agaaaccttg
    28501 aaggatgcca ttcatacgca agtttcaaat gaacttcctg aaagcattat ggacagtata
    28561 cgagaagtac gtgattattt tgaagagtct accattcttc agggaattga cgtgatttat
    28621 gatcgacagt ttttgacaga gcagcgacgg cttgccgata agctaggata tcaagaattg
    28681 ctgacagaag tcattgcttt tattgacttg accaatatca caacgatggc tcgaggaata
    28741 ttgcagaaaa gaacatatgg gttcatgacg acggtacttt ccagctcggg aagtatcgaa
    28801 aaagaagtat ttttgaactt tgtagataaa gatatggaag attacacgaa ttttctatta
    28861 gagacagatt atgctgattt tctgcgccct gtcatccgtg agggaaatat tgatctcgta
    28921 aagcttgaac agttgaaaga tgattatcta agttcattgt atcaagaagc acaaacccag
    28981 gctttcggac cactgccact actagccttt ttgaacgcaa aagaggtaga aagcaaaaac
    29041 cttcgtttac tggttatcgg caaaagaagc catttcgaat cagaaacaat cagaaagcgg
    29101 gtgagacaag tctatgacgc ataagatcgg tgtagttggc gacaaagatt ctgttttgcc
    29161 attcaaattg tttggctttg atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc
    29221 aattgaagaa atggcaaaga atgagtatgg cgtgatttat gtcacagagc aatgtgcagc
    29281 attgagccca gaaacgatcg aacgttacaa ggaacaaatg atacctgcta tcgtattgat
    29341 tcccaatcat caaggatcat tagggatagg gctggaagaa attcaaaaaa acgtagaaaa
    29401 agcagtaggt caaaatatct tatagtatga aaaggagata gaaattttgc aaattggaaa
    29461 aatcataaaa gtctccggac ctcttgtaat ggctgagaac atgtctgatg ccagtatcca
    29521 agacatgtgt ctagtgggag atctaggagt tatcggagaa attatcgaga tgcgtggcga
    29581 tgtggcgtct attcaagtat atgaagagac ttctggaatt ggtccaggcg aaccggttcg
    29641 ctctactggt gaagctttat ctgttgaatt aggaccaggg atcatttcac aaatgtttga
    29701 cggaatccaa cggccattag atacctttat ggaaatcaca caaagtaatt tcttagggcg
    29761 cggagttcaa ttgcccgcat tggaccatga gaaaaaatgg tggttcgaac caacagtaga
    29821 agccggagaa acggtgagtg ctggagatgt tatcgggatc gtagaagaaa caaaagttat
    29881 aaaacataaa atcatggtgc caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg
    29941 ttcattcact attgatgaaa cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt
    30001 gacgatgctt caaaaatggc cagtaagacg tagtcggcca atcaaagaaa aattaaaccc
    30061 ggatgcaccc atgatcactg gacagcgagt gattgataca ttcttcccag tgacaaaagg
    30121 aggagcagca gctgtacctg gtccttttgg tgcagggaaa acagtcgttc agcatcaaat
    30181 tgcaaaatgg gccgatgtgg acatggttgt ctatgtggga tgtggcgagc gtggaaatga
    30241 gatgacggat gttttaaatg aattccctga attgattgac cctaatacag gagaatcgtt
    30301 gatggaacgt acggtattga ttgctaatac atcaaacatg ccagtagcag ctcgagaagc
    30361 ttctatctat acgggaatca caattgcgga atatttccgt gacatgggtt atgacgttgc
    30421 tatcatggca gactccactt cacgatgggc agaagcactt cgtgagatga gcggacgttt
    30481 ggaagaaatg cctggtgatg aaggttatcc agcttatctt ggttcacggc ttgcggaata
    30541 ctatgaacga tctggaagag tcattgcatt aggatcagaa ggtcgcgaag gaagtataac
    30601 cgcaatcagt gctgtatccc cgtctggagg ggatatttcg gaaccagtca cacaaaatac
    30661 attgcgggtc gtaaaagtat tctggggtct tgattccagt ttagcccaaa aaagacattt
    30721 tccttcgatc gattggatac aaagctattc tctttattca acagaagtag ggaagtatat
    30781 ggatcatatc cttcaagctg actgggcatc catggtcacc gagggcatgc gtattttgca
    30841 ggaagaacag cagctgaatg aaatcgtgcg tttggtaggg attgattccc tttcagataa
    30901 tgaccgtctg acattagaag tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc
    30961 ttttgatgat gtcgatacat tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat
    31021 cttgaccttt ggtgaagaag gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat
    31081 catggacggt accgtatcga ttcgtgaaag gatcggacga agcaaatata tcccggaaac
    31141 agaattagaa aaactagatc aaattattgg tgaaatcaag caaacgatgc aaacgatcgt
    31201 ttcagaagga ggcatgagca atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc
    31261 cattgatggc ggttgaaaaa gttgctggtg tcaaatatga agaattaatc gaagtccgta
    31321 tgcaaaatgg cgagattcgt cgtggacaag tattggaagt ccaagaagat aaagcaatgg
    31381 tccaaatatt cgaaggaaca agtggtatca atttacgtga ctcttctgta cgatttttag
    31441 gccatccttt agaactaggt gtatcggaag acatgatcgg tcgagtattc gatggtttgg
    31501 gacgaccgaa agataacgga ccagagatcc tgcctgaaaa gcatttggat attaacggag
    31561 aagtaatcaa tccagtagct cgggattatc cagatgagtt tatccaaaca ggtatctctg
    31621 caatcgatca tttgaataca ttagttcgtg ggcaaaaatt accagtattt tctggctctg
    31681 gtcttcctca taaagaatta gctgctcaga tcgcgcgaca agcaacggta ctcgattcat
    31741 cagatgattt cgccgtagtc tttgctgcta ttggaatcac ttttgaagaa gctgaatttt
    31801 tcatggaaga ttttcgtcaa acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag
    31861 caaacgaccc tgcaatcgaa cgtatcgcta ctcctcgtat ggctttgact gctgcagaat
    31921 atcttgctta tgaaaaggga atgcatgttt tggttatcat gacagacatg accaactatg
    31981 cagaggcatt gcgagagatt tctgcagcac ggcgagaagt tccaggaaga agaggatatc
    32041 ctgggtatct ttacacgaac ttagctactt tattcgaacg agcaggaaga atccgcggac
    32101 taaaaggatc ggttactcaa attccgattc taacaatgcc agaagacgat aaaactcatc
    32161 caatccctga cttgactggt tatattacag aaggacagat catcttgact agagagcttt
    32221 acaagagtgg gattcagcca ccaattgatg tactcccgtc tctttcgcgg ttaaaagata
    32281 agggaactgg acccggaaag acaagagaag accatgcagc tacgatgaat cagctttttg
    32341 cagcctatgc acaaggaaaa caggcaaaag aattagccgt agttttaggt gaatctgctt
    32401 tatctgatgt agacaagatc tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca
    32461 atcaaggatt ctacacgaac cgttcgatta ctgaaacact tgatctaggg tgggaactgc
    32521 tatcgatgtt gccaagaacc gaattaaaac gaatcaaaga cgacatgctg gacaaatatc
    32581 tgccagaagg gaagtgagtt ctgatggcac gattgaatgt gaatccgacc cgcatggaat
    32641 tgactcgatt gaaaaaacag ttgacaactg ccacaagagg gcataaatta ttaaaagata
    32701 agcaagatga actgatgcgt caattcatcc tgttgattcg aaagaacaac cagctgcgtc
    32761 aagaaatgga agagtctatg acagaagcaa tgtctgattt tgtattggca aatgcttcga
    32821 tcacggaacc tttcatcgaa gaattatttg ttttgccggc ggaaaacgtt gatttgtcta
    32881 tcgtagacaa aaatatcatg agtgtaaaag tacctgtgat gaattttgaa tatgatgaac
    32941 agctgacaga cacacctttg gaatacgggt atttgaattc cagtgccgaa ctggatcggt
    33001 caatcgatcg gtttactacc ctgcttccaa aacttttgga actgacagaa atcgaaaaaa
    33061 cttgtcaact gatggcgagt gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat
    33121 atatgacgat ccctcaatta gaagaaacga tctattatat tcgtatgaag ctggaagaaa
    33181 acgagcgcgc agaagtaaca aggttgatca aagtaaaaaa tatgggtacc actgactaag
    33241 tggtatcttt ttttaaaaga ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg
    33301 tttgccggct gttcagcttc tcgcattagg attttttctc ctgattttag ttggcggcgg
    33361 cctacttacc ctaccatttt ttagtaatag tgggcatggg acaaatttta tcgatgcgct
    33421 tttcactgca acttctgctg tctgtgtaac gggattgaca acattgaata cagcagagca
    33481 ttggaacagt gctggtcaat ttttgatcat gcttttgatt gagatcggcg gtcttggttt
    33541 tatgatggtt ccgattctat gctttgcatt gataaagaag aaagtcagtc tcagcacacg
    33601 tattatatta aaagaagcgc tgaatctaga agaagtatca ggtgtgatga agctcatgct
    33661 ttacatccta aaattggcct ttgtgatcca attgattggt acggttgctt tatcttttgt
    33721 atttgtgcct gaatttggct ggttgaaagg tatctggtac agtttgttcc atgcaatctc
    33781 tagtttttgt aatgcagggt ttgatttgtt aggagacagt ttagccaatc accagcaaaa
    33841 tgtttatttg atcatggtgg tatctgcttt gatcatttct ggtgggttag gcttcatcgt
    33901 atggcgtgat ttactggaat atcatcagaa aaagaagatg acgattcatt caaaaatcgc
    33961 attaaccgtt acaggtaccc ttttattagg cggattcgtt gtgttctttt tcacagagcg
    34021 caatggggca acgctagtgg aagggaatat gtttcaacga ttggcaaaca ccttttttat
    34081 gagtgtgaca cctagaacgg ctggatatta ttcagttgat tacctccata tgagccatgc
    34141 aggtctgatt ttaacgatgt ttttgatgta catcggcgga acatctgggt cgacggccgg
    34201 cggattgaaa acaactacgt taggtgtgtt gctgatccag atgcgttcga tgttgaaagg
    34261 acgtacgaga gcggaagcat ttggccgtac catccgacaa ggcgcagtgt tgcgggcact
    34321 tactttattt tttgttacgt tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac
    34381 tgaaacgata cctaaaactt ctggcatcga atatattgct tttgaggtct tctctgcgtt
    34441 tggtacagta ggtcttacga tgggattgac gccagacttg actgcaatag ggaaattgat
    34501 cattatagcg cttatgtaca ttggacgagt tggtgtattg actgtggtct tctctctgat
    34561 gacaaaagca caaagaacac aatcaaaata caaatatcca gaagaaagtg tcatgattgg
    34621 ataatttcat cttctgacag aaacctagtg atttagtaaa ataagaaaat aaaataattt
    34681 ttcttgcttc ttggatactt taagtgcaaa atgttcgttt attttttgag gcactacccg
    34741 ttgttgaacc tcgggtagtg cctctttttt attgaaagga atcaataaga tttgattcct
    34801 ttatttatca ataggttata attcatattt ttttattttt tcttttacga tacttagtcc
    34861 agaagctaaa atttcaaggt tattagagga tagggaaatt cgaacgaatg catctggttg
    34921 cgcacgtgta gtaaagcgtc tggaatgata aagtcggata ccgttagcaa gaaagtcatt
    34981 ctcaattatc ttagggtcaa tctcaggatc aactggaaga attcgatagt aagggacacg
    35041 attcgttggt ttttctactt tgaaaagaga atcaaaaagc tgattggctc tttttgtaag
    35101 acttaattta gtttctaaga tcctatttgc ttcatttgaa tggaggattt gagtgatgac
    35161 ttccacatct aatccagaag ttttcacatt tatattaaaa attgcttgtt ctatcttgct
    35221 ttggacatgt actggaacta caagataagc aactcgcaat ccagaacaaa taaattttgt
    35281 cattccagct aagtagattg tttggttagg taataaattt tggaaaggag gaatgatcgt
    35341 atgttgatag taagttgtta aaaatgaatg gatatcatct tcaataaccc atagaccttc
    35401 tttttcaatc acttcggcta attgctctcg tcttttgtga gagatttgaa accctatagg
    35461 attattacaa gaaggcataa gaaagacacc atgaatcttc ttttttctac attcctgaat
    35521 caatatatca ggtcgcattc cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata
    35581 taactgagcc aattcaatga aattagcgta tgtatatcgg tctgttgcta ttctatcacc
    35641 tggagagaaa agtgcagaaa gtgtgatagc caatccattt tgaacaccgg aaacaataga
    35701 tagagattct aaggacggaa tgactccttg agtttttaac caatttgctg cagtagtcaa
    35761 ttgaaaattt gtgcctttgg gatcttggta attaagcaaa ccaacaagat ttgggttatt
    35821 actagttgat tgaataaacg ggacaacgaa tttattacat tcttcaaaag aactaaccag
    35881 accaaaatca atgatatgtt cggttacttc attggtagag atggtgatgg actcaaagac
    35941 attgggagaa ataaatgtgc cacttccaat attcgtatac agcaaacctt tctcaattcc
    36001 atatttataa gccttgccaa cggtagtaaa atttaaatct aaataatcag ctagctctct
    36061 ttgggagggt aactgagtgt ttttctgtaa tttattcttt aaaatatctt gttctaattg
    36121 ttgaatcaac gatcgataat aaggacgctt caatttttgt ttgtctggtc gccaagttaa
    36181 cgggtaattg tcataagaat taataggcat tttctccact ccttaaaatt gtataccata
    36241 caattttacc attgattgta ttgattttag aagttaatct tttgtgagga gggaattgtc
    36301 tatgaaaatt attaattttg aggaagtagt agcttctttt agttttgaag aagcgattca
    36361 agtaatgaaa aaatgttttt ttgattatga aagtggtaaa atcagccaaa atcctcgatt
    36421 ggtggaaatt ttacctgacg gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt
    36481 gggggagaac agatattttg gcacaaaagt cattaccgct tttccggata atcatcagta
    36541 tcatttacct tctcatttag gagagatttt cttgtttgat gctcaaaacg gactacccaa
    36601 agcattgata aacgcaaatg cagtcacctg gattcgaaca gctgctgtat ctgcgctggc
    36661 aacagattac ttagcaaatc cgcatgcaga aacacttgct ttaattggag ctggtcaaca
    36721 agcattgtcg catttgactg cgatttctac cattcgtccc atcactacag tgtttgttta
    36781 cgatatttta gaagaaagac gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa
    36841 aataacgttt ataaatgcaa tatcagtaga agatgcagta cgtccagcgg aaatcatttg
    36901 tacgttgacc tctagtaaag aggcatttct tcaaaaagag tgggtaaggg aagacgcaca
    36961 tatcaatgct gtgggcacat ttactcctga tacaagagag ataagtacat cgttgatgga
    37021 aacttctcaa atctatgtag atgattactc agctgcctta acagaaagcg gggacctgct
    37081 tatagccatg gcagaaggag atttagagga atcttgtatt gttggtacat taggcgaatt
    37141 agtttctgag aaaaaaacat tttataaagg agaatcgagg ccgacgattt tcgacgcagt
    37201 tggtctagct attgaagatc tttgttgtgc agaatatatt tataacaaat tagagaaagg
    37261 ggctttaaaa tgaacttaga ttattacgta gttgatgcat ttgcagatga ggtgttcaaa
    37321 ggaaatccag cagcggttta tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa
    37381 attgccattg aaaacaattt atctgaaaca gcatttacag tgaagaaaaa ccaagaattt
    37441 gagcttagat ggtttacgcc agatcgagaa atcgatcttt gtggtcacgc gacattagca
    37501 acagcatttg ttttatttaa ctattacaaa atacctgatg aaacgattaa attttctact
    37561 caaagcggta atctatttgt gacaagaatt aaagattatt attacatgga ttttcctagt
    37621 attatgccga aaaaagtgcc gatattgtct gaatatgaag aagcgattgg ggctaagatt
    37681 aaagaagcct acttagcacg tgatttattt ttcgtattag aagatgaaaa aacggtggct
    37741 aaattgcagc cagattttac agctatcaaa aattttgaat taggaatagg agtcatcgtg
    37801 acagcagaaa gtgtgcaaaa agactttgtt tctagaacct tctttccaaa attaacaatc
    37861 aatgaagatc cagtatgtgg atcagcacat tctaatctta ttccttattg ggcagaaaga
    37921 atgaataaaa caaaattgtc agcttatcaa atgtcatcaa ggggcgggga tctagaatgt
    37981 gaattaaaag aagatcgtgt gataatagga ggaaaagcag ttttgttttc taaaggtacc
    38041 tcgtttattt gatttctttc aaatgaatta aaaataaatt ggtggagagt ggcaacctta
    38101 tagctacaat ggttgttaaa aaaagacgca aactttttca atttaatgaa aagtttgcgt
    38161 ctcattttaa ttattggtgt ttgtttttta ttcaatcaag agtaaaatgc cccatactaa
    38221 ctatcctcgg atgacttcga ttttataacc atcagggtca atgacaaagt agtaagaagg
    38281 cgcagtgcca ggtaatcctt ttaagtcagt aatctcaaga ccagcagctt tatgttgctc
    38341 gtgtaatttt tctagatcat cagctgctat agcgatatga ccatagccgt ctcctagatc
    38401 atacgcacca tgatcatagt tgtaagtcaa ctctaattca tagtcatccc caggaagtgt
    38461 caaataaaca agtgtaaatt tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc
    38521 aaatgccttt tgataaaatt cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg
    38581 agccattttc atagattcat ctctcctttt ccttttactc aatcataaca aaaagaagta
    38641 aggaaatagt tgatttgtgc ttgttcatga gaaatttctc atgtaaaatg gatagaaaat
    38701 aaaactgtta aggtggaaac tattcatgga taaaccaatt tttggtgaaa aaaagcaatt
    38761 tgctgattat caaacccgtt atgcagcata tattgtcata gaaagagatc agcagattgc
    38821 agtaatcgag gctccaaatg gtgctttttt tcttcctggc ggggaaattg aaggagaaga
    38881 gacgaaagaa gaagcgattg tccgtgagct gatagaagaa atgggaattg tggcagaaat
    38941 ttcttcttat ctgggagaag ctgatgagta tttttactcg aattatcgtg caacttatta
    39001 ttataatcca ggatatttct ttgttgctga ttcttggaag cgtataggag aaccaactga
    39061 aaagaccaat aaaatttggt gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg
    39121 tagccaccga tgggcagtcg agagatggat cgaaaaaaat aaaaactgaa agaaagcgag
    39181 tagatcagct ttctctcagt ttttttattt attacagtac atattttttg attgcttcag
    39241 ctacaccgtg ctcgtcgttt gatgaagtga tgacatttgc tgcttctttg acaagagaaa
    39301 cggcatttcc cattgctact cctagaccag catattcaat catggataag tcgttctcgt
    39361 tatctccgat agccatgatt tcatcttgat caataccaag gtgctgtgcg agattcgcaa
    39421 ctgccgcacc tttacttgct tctttattta gtacttcgaa gtaatagggc gcacttttga
    39481 ccgttgtgta tttatcacgg aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt
    39541 ctggttcgtc gatcatcatc attttgacga tgttcatgtc tggtgtcatt tcttcaggtg
    39601 ttcgatattt tagcggcatc ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat
    39661 ctcgattagg cgtgtagatc gctttgtcat cgatcgaatg gagatgactg ccgacacggc
    39721 gtgccattac ttcgatctct aggaaatctt catgtgtcag tccgtgacgg gcgatgattt
    39781 tacctgattt cgtttgttgt acgagtgccc cgttataagt aataacgtaa tcattatctt
    39841 gaaaaaggtt caactcgttt aactgttcct ggacacctgg taagggacgg ccagtgcata
    39901 aaacgatcga aacacctttt gcaacagcag cggcaatcgc ttcttttact tcttttgtta
    39961 cttctctttt ctcatttaat aaagtaccat cgatgtcgat agcgactagt ttgatagaca
    40021 taatttcctc caatatattt taatttattt caatcaatgc gccattacgg atatggctgg
    40081 caaattcttg ataagtttcg tcaaataaat cataatgatc acgcaaggac ggatcaacca
    40141 tttcttttgg aaagaaaaat cgctcatctc cttgttcttg tcccgctaat gcagcaacaa
    40201 gctgactaac ctgtgataat tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg
    40261 tgcgatgtct gccttgttct gggcgataaa aatcataagg tagatcgtag ctagaattca
    40321 cagcagtgta ataaacagga ttatatccga ccttttctac taacaaagtg agttcttgga
    40381 ctaattttga atcccggttg ccagaaaaag tagcagattt cagtggttta cgattcaaaa
    40441 aacgtttagc taaatcattt aaaataggat cagcagattc agaccattga gtaaagtaag
    40501 tgcccaagac cccatcatca agtttgaggt attcttctaa agtaaagctt ccgtcaagaa
    40561 atggcagaag taattgagaa tggagttcaa aactgctggc atcttcttga tacaactcgt
    40621 gtgcgcgatg aagcagatgc tccaaaatta cttccatacc acgggaaaca ggatggaagt
    40681 acacttgcac atacatttga tatcggctaa cgatataatc ttccactgca tgcatgccac
    40741 tcatggaaaa agcgatcccg ccttcataag gacgaatcac acgtaatatt cgggtaagat
    40801 caaatgttcc atattctgtt ccagtgaaat aagcatcccg aagcaaatag tccattcgat
    40861 ctgcatcgat ctgactagaa atcatttgga caacttgtgg gttggggtaa gttttttgga
    40921 tcacactagc taccttttca ggaaaacctt cttctactcg gttaagaatc tgatacactt
    40981 ctgtttctgg agaagtgatg atctctacag tgatggcttc atgattcgta tggaagatat
    41041 gttcaaatgt atgagagtaa ggaccatgac cgacatcatg caacagtgcc gcacataaag
    41101 tcaccagccg ttcctgatca tcccagcctc cgttgcctat tttttctttt gaaaaatttc
    41161 gagaaaagat atcgcagatt cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac
    41221 ggctatgttc tgctccgtga aaagtgaaag aagaagtgcc taattgttta atgcgtcgta
    41281 gacgttggac ttctttcgaa ttgatcaaat ctaagattac ttgatgctgg acatggatat
    41341 agttatggac tggatcacga aaaacttttt ccataggcag catttgatat ttataagggg
    41401 ttgtcataat gtagctcctt tatgttatta cgttgttcca ttctctggat ttggttgttc
    41461 cattgctcca gctggatttc tttttttagc cagtcggtag aatcctgtaa atcacttgca
    41521 gaatacagct tttcaaaagc ttttgcaaat gcttctttga cttcttttac aaaaaggggt
    41581 tggtcaagca gttcttcaag tgttgccatt gtagcaggat ccactgcagg atagccattt
    41641 gtaccaaagt cttcttttaa agcagattga taaaatcgct tcatgacttg cccacgtttt
    41701 tcctgattac cgttgacact taagtacatc ataacagaaa tcccattttt gatccgacgc
    41761 tgagcgatac cggcaaattt tttgccatgg atgcttaaat cataagttcc aggacaataa
    41821 gaatgggtga tttcgaatgc ttgtatcggt gcctttggaa aagcgtattt agtcaatagg
    41881 agcatttgct cgtaagcgtc atctatggtt attttttttg tgtcactttg ggggaaaatc
    41941 aaagaaatat tcaaaattcc ttcatctgat acgatcccta atccacctga atttctgatc
    42001 actggagcgt aacctgcatc ttccagtgtt tggatcccgt cacttagaaa aggagttcga
    42061 acatccttca tacctaagat gactgtgcag tcatattgcc agaaatgtaa aaagatttgc
    42121 tggtgttctt tagaatattc cgttaatata tctgttaatg caaaagggga aaagtcgttt
    42181 ttttgataaa tgtgctgatc tagtcgaatc gcagggttca ttcaaccggc tcctttgtat
    42241 aaattcatat tttctacaat attatactct aaaatcaaag agttgttttg gaaaaaaggt
    42301 tttctgtaaa aatggacaag ttggttgaca aaacaaaaga aactaggaac aatgtagcta
    42361 tgagaagtaa aggagaatga ataatggaat tatcacaagg aacaccgatt tcaatcaaat
    42421 tgcgtacaaa agtaaaacag caaggggaag tgcaagattt ttattttgat ttgaaagggc
    42481 aaatggtgac aattggcgat acgctgtaca tacgttataa agagattcag gaggagacgg
    42541 gcgatgagat tcctgtaacg atcaaattag ttccagatgg tcatgtacag ctgatccgtg
    42601 caggagaaat gcggatgcgt ttgaaatttg gctataaaga acggttagaa acatcctatc
    42661 gaacaccata cggtatgatc cagattgcta cttttacgaa agaactgcat gttagtttga
    42721 aagaccgtcc cacagcaggg aaggttcgaa tcgattatga tctgttcatg ggaccggaaa
    42781 aaatcggcga atattatttg accttggatt ttacagcgta aatataaaag aaaagtttga
    42841 ttttttgtgt ctgattttgt atgataagga gtagactgtg aaaggacgtg tcctgattgg
    42901 aaattagtgt atttgaaggt gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg
    42961 cgatcttaga acaacgtggc gatgtcatgg atttttctga tttagctaac cagatccaaa
    43021 actatcttgg gaaatcagac agtgaaattc gtgattcact cgcacaattt tatactgact
    43081 tgaatattga cggcagcttt atctcattag gggataatcg ttggggcttg cgttcatggt
    43141 atgctatcga ttcgatcgat gaagaagtaa accatggaat ggacgaagag gatgaagata
    43201 ctccacgtcg ccgtaaacgc aagaaagtca acgcctttat caatgatgat gaagacgcga
    43261 tcgattacaa tgacgatgac ccagaagata cagatctgac agaagaagat gacgatgatc
    43321 tgtttgatga cgatgatgat gaagatgaag aaatcgctgc ttataattct gacttgcaag
    43381 aaatcggagc agatgacccg acagatgatg aagaagactt gccaggaaat atcgaagaag
    43441 atttaagtat catagacgaa gacgatgatg atgaagatta tgaagaggaa gaattttctg
    43501 actctgaaaa agaataaaca ttattttctt acgagaggtt gtgacagaga cgatcagcct
    43561 atcagaataa ggaggtgtga cacgattttt gtcacacctt cttttttgta tataaaaatc
    43621 ccaagtaaat acataaaaaa taaaaaaatt gaaactttta gcgcaccttg tacgtcatat
    43681 taagtgaggc agaaaggagt ggagaatgat ttttactgga tataagaaaa aacggcagat
    43741 cgaaaaatac cgtcacctgc tgaatggact catcgaaact gattatcaaa aaatgttccg
    43801 tatcacgatg aattacgtac atgataaaga agaggcctta gatgtgatgc aagatagttt
    43861 tcataaagcc ctgaactatt ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt
    43921 ttatcggata ttgatccgca cggcattaga tagttggcgc agacaaaaaa ggacgcctgt
    43981 ggacttattt gaaattcaag atatccccca acagttacct ttagaaactt ccgttgcaga
    44041 gctccatatg attttagata aaatagacag tccggaaaaa gagattctga tcctctactt
    44101 ttttgaagga tttcgtttga aagaaattgc gactatcttg gatatgaatg aaaacacagt
    44161 taaaacgaaa atgtaccgct cattgaatgc tttacgtcaa atcctagaat gaaggagaga
    44221 agagtgccat gtattcccaa aaagaaaatt taaaaaaact aaagaaaact tataaagaac
    44281 aagcaattcc caaagggctt aaagaggaga tccgccagcg ttttattatc gaagagcaat
    44341 catttataaa agcaaagcgg agaaagagac gtctccaagg gttcggagta tctttggcta
    44401 tcgtaatgat tacaacaagt agccttcttt ttaatagtca agttcgaagc tttgcagaag
    44461 atttacccat cttaggatca gtcattgaat tgatcttagg cgaacgcttt actgatcgat
    44521 ctgaaaaaat cgatattcag gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa
    44581 caattcatgg attaaatcaa aaatactttc gcgaaggaca agcagatttt gaaaaagctg
    44641 aaaaagaata tggaaatttt gaaacggatc attaccaagt tttgggagat tatcagaaaa
    44701 tcgtagacga taaccggttt ctcgtgattg aaaggcagat cacccaaaca gcagcagata
    44761 gccatgttga aaaaagatac gacacaatcg acaagaaaaa cagtgtccag ctctcacttc
    44821 cacttttatt caaagatgac acctatctat ctgttttgac aaaagaagtc aaacggcaga
    44881 tggccgaaca agtcaaggaa gatccatcta agtactattg gactgagcaa gatattcaag
    44941 aagggacgat agaaaaacca acacttgtta cgcctacacg aagtttttat ttgaacaaag
    45001 agcatcaatt agttttgact ttttctcaat acgaaattgc tcctggttat atgggtacac
    45061 cagaatttgt cattcctaag tccgtgacaa aaacgatttt ggcatcggaa gattatttgg
    45121 atcattagac gagaagtagt tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa
    45181 taataagaag agaaaaagag aaagaagaga tatagtgaaa agaaattatt tagttatcag
    45241 tattattgct ttatccgtgc ttttgttttt cgaaattact tcggtggcct ccttgatcac
    45301 cgaagtaaaa ggacaaacac actcttcgga gcattcgtca caatctttca aacaatcaga
    45361 acaatcaacc attaaaagcc aatcaactga aacagctaac gtcaaagaag ccattcaaga
    45421 atcagcggaa aaggtacaaa gtcagtcatc acaatcaaca caagacagta caaaaacgag
    45481 ccgagatccg gctgtcaaaa gcgtcgcaat cagctttgat gatggaccag gagcgaccac
    45541 aacaccgcag cttctgcgaa tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt
    45601 aggagaaaat acggcgcagc atcctgaaat cgtgaagcag acagctgaag cagggcacga
    45661 gattggcaat cacacatacg atcatcagaa tctggccact ctttctgccc agtcgatgac
    45721 ggaggaagtg acgaaagcag acactgagat caaaaaagtc actggcaaaa cccccacttt
    45781 tgttcgtccg ccctatggtt ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat
    45841 catcgaatgg tctgttgatt cagaagattg gaagacaaga aatcctgatt tgattttgca
    45901 gaagatccaa gcaacagtat acgatggggc aattatttta ttccatgata tctatcctga
    45961 aacgattcgt gccgttcctc aagtgatcga ttatctgaaa gagcaagggt atcgcatcac
    46021 gacagttggg gatttattag ggcatccaac ggctgtcgag aactattatg gcagaaacga
    46081 tcatcggcca gttcagtaag tgagacaagt atctttaata aataacacat tttaataagt
    46141 agttgaacac ataactacct tacttgcaaa ggcttaacaa tatctataca ggtacaacaa
    46201 aaaactgtcc accaattttc tttggcggac agttttcact taaaaataaa gaagcaacta
    46261 ttcgtaaatt atgctaaaat agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc
    46321 aagaaccgga atcttacgtg atatccacta cactaaggac gcaagcactg ggataattgt
    46381 aactaaacag ataaaaaatt acaaggggat tggacaaaaa aatgaaattt gagcaaaatt
    46441 tttcacaaaa acagcagcag acacagaaac tagccatgac gcaacagttg caacagtcca
    46501 tccaagtttt acaatataac agtgaggagc tgttggcatt cgttgagaat caagcaatgg
    46561 aaaatccatt ggtggaagtt gttgagccgg aatggcagcc agattacata aaagcttctt
    46621 catcttctta tgaaggagaa gagacaaatt acctaaatca gattcctgat acaaaagggt
    46681 ctttatttga ttcattgatc gaacaagtcc atttgaatta ccgagatact tttttgagga
    46741 aaatcgttct ttatcttgta gaatacatcg atttgaacgg atttttgacg attagtttag
    46801 aagaagccgg aaaagagatt ggagctacgc ctatccaaat gctcgatgcg ttgactctga
    46861 ttcagcaatt agaaccagct ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc
    46921 aaacagaacg agatgattat gcgccggaat tagcttatat cgtgttggaa gaatgttttg
    46981 aggaactggt ggagcgaaaa tggaaagaaa tcgcacaacg tttttctgtt gatctacatg
    47041 ctgttcagca gatattcgac tatttacaaa cgttgagtcc aagccctggc cggattttcg
    47101 accgtagcag cgaattgtac attattcctg atgtccgggt attagtggac gacgataaaa
    47161 atgtacaagt tatctcaaac cgaaaaaatc agccaaatat acgctttcaa gagagttact
    47221 tcaaacaaat gtcacaacaa gcagataaag aaactgaaaa ttatttaaaa gaacggaaac
    47281 aggaattcga atggttgaag aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg
    47341 ctcaagtcat tgtttcccgg caaaaagctt tctttatcga caaggaacgt ccaatcaagc
    47401 ctcttacgtt aaaggaagtt gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg
    47461 cagtcaacgg gaaatatttg gaaacagact tcggtgtatt tgaattaaaa aaatttttca
    47521 ctacacgaat tcctacgaat agtacagaac agacagaaga cctttcagca gatacagcga
    47581 agaaaaagct gcaggaatta gtcgatcaag aagacaaaaa caagccgttg tctgaccaga
    47641 aattggttga attgctgaaa aaagatgaga tagcgatctc aagacgaaca gtagcaaaat
    47701 atcgtgattt attaggaatc ccgagttcat caaaaagaaa acgttatgat aactaatcag
    47761 tcaaaagact agaaaaagta tttcactttt tctagtcttt ttagttgaaa aacgagagca
    47821 aaactgttag acttacagtc gtgaggttga gatttccctt tattgatgtg aaattcgaat
    47881 agttaatttc tttttttatc gtatatgggt cgttttaagt ctacattgga cgtaaagtgt
    47941 ccaactaagg agatcgctat gctggatgaa ataaaaatga ttgaatctgt tgcaccagat
    48001 attatcgaag tgttgcaaga acgctataaa attttacgaa atatctattg gatgcagccg
    48061 attggacgtc gaagtttatc ggaaagtatg gggatcacag aacgagtatt gcgaacagaa
    48121 acagatcttt tgaaaaatct gaatttgatc gacacctcaa aaagcggtat gacgctgaca
    48181 actaagggcg aagaagttta tcaaagcctt gaaaacttca tggatcagct tttaggtacg
    48241 catcaaaccg aacaacagtt ggcagaatat tttggtaacc aacgctgtat cgttgtttca
    48301 ggaaacagcg aagagcagac caaagtggct gatgcattcg gcgaagcgct gagcgaagcc
    48361 ctagaccgac tacttccaga aggcgagaat atcatcgctg tgatgggagg aactactatg
    48421 gctgtggtag ccgaacaatt gtctaatctg gaaaataaga aacggcataa cctgtttgtc
    48481 cctgcaagag gcgggattgg tgaagcgata actgtacagg caaattctgt cagtgcaaga
    48541 atggctgcta aagcaaacgg taaccaccgt gcactttatg ttccggaaca attaagtctg
    48601 gcaacttaca attctttact caatgagcca tctatccaag aagtcttaaa cttgattagt
    48661 gaagcgaatt gtgttattca tagtattggg cgtgcattac acatggcagc acgtcgtaag
    48721 atgactgaaa aagaattagt catgctgaaa caagcaaatg ctgttgcgga atcttttggc
    48781 tactttttca atgaaaaagg tgaagtcgtt tataaggtcc ctagaatcgg catagaattg
    48841 caagatttgg aaaaagtccc gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa
    48901 gcaattcgag cttatatgaa aaatgcacca aaacaaacgt ggctcatcac tgatgaagct
    48961 gctgcaaatg agattttaaa aggggtaacc ctttaaaata aatatttttt tgattttcat
    49021 aaggaggaaa tcttaaatga cagttaaagt aggtattaat ggttttggac gtattggacg
    49081 tctagcattc cgtcgtatcc aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt
    49141 gacagatgct aaaatgttgg cacacttgtt aaaatacgac acaactcaag gacgtttcaa
    49201 cggaacagtt gaagttcatg aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt
    49261 agctaaccgc aacccagaag aattaccatg gggcgaacta ggcgtagata tcgttctaga
    49321 atgtactggt ttcttcactt ctaaatcagc agctgaaaaa catttaacag ctggtgctaa
    49381 acgcgttgtt atctctgctc ctggcggaaa cgatgttcca acaatcgttt ataacacaaa
    49441 ccacgaaaca ttaactggta aagaaacagt tatctcaggt gcttcatgta ctactaactg
    49501 tttagctcct atggctaaaa cattgaacga caaatttggt gttgttgaag gattaatgac
    49561 aactatccac gcttacacag gtgaccaaat gactctagac ggacctcatc ctaaaggtga
    49621 cttccgccgt gcacgcgctg ctgctgcaaa catcgttcct aactcaacag gtgctgctaa
    49681 agctatcggt ttagtaattc ctgaattgaa cggtaaatta gacggagctg ctcaacgtgt
    49741 tcctgtacca acaggttcat taacagaatt agtaacagtt cttgaaaaag aagtaactgt
    49801 tgacgaaatc aatgcagcaa tgaaagaagc ttcaaacgaa tcttacggat acaacacaga
    49861 cgaaatcgtt tcttcagata tcgttggtat gacttacggt tcattatttg atgctacaca
    49921 aactaaagta atgacagttg gcgacaaaca attagttaaa actgttgctt ggtacgacaa
    49981 cgaaatgtca tacactgcac aattagtacg tactttagaa tacttcgcta acttataaga
    50041 tagacaaacg tacaagtgta aattatagac atgtacattt aaaagcgggg aagcaatcgc
    50101 gcttcttccg ctttttttga taataagttt attcaggagg aaataaaatg gctaagaaaa
    50161 cagtgaaaga tatcgattta aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc
    50221 ctttgaaaga cggcgtgatc actgacgaca ctcgtatcaa agcagcattg ccaacaatca
    50281 actacgttct agaacaaggc ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa
    50341 cagaagaaga taaagaagga aaatctttag ctccagttgc aaaacgtcta ggcgaattat
    50401 taggtaaaga agtaacattc gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc
    50461 gcaacatgaa tgacggcgac gttgtcgtat tcgaaaatac acgatttgaa gatattgacg
    50521 gtaaaaaaga aagcaaaaat gatacagaac taggtaaata ctgggcttca ttaggcgatg
    50581 tatttgttaa cgatgctttc ggtacagctc accgtgcaca tgcttctaac gtaggtatcg
    50641 cttcaactgg tatcccaaca gttgctggat tcttgatgga aaaagaaatc aagttcatcg
    50701 gtgaagcagt agaagaacca aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt
    50761 ctgataaaat cggcgtaatc gaaaacttga ttcctaaagc agataaaatc ttgatcggtg
    50821 gcggaatgac ttacacattc tacgaagcaa aaggaatcaa gatcggtaac tctctagtag
    50881 aagctgataa agtggaacta gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct
    50941 tgccaatcga caatgtctgt gcaccagaat tttcaaatga tgttgaaaca caagtaatcg
    51001 aaggagacat tcctgatggt ttgatggctt tagacatcgg cccagcatca gtaaaattat
    51061 ttgctgatac attacaaggt gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg
    51121 aaatgagtaa ctttgctaaa ggtacaatcg gtgtttgtga agcaatcgct aacttagaag
    51181 gcgcaacaac aatcatcggt ggcggagact cagctgcagc agcagaacaa ttaggatttg
    51241 ctgacaaatt cacacacatc tcaacaggtg gtggcgcaag cttggaattg cttgaaggta
    51301 aagaattacc aggactagca gcaatcaacg ataaataaac gaccaagttt gctcaattct
    51361 gacgagtaat cgatcaaaag gtgaagggag ttttcatgac tccctagcca tttttcaata
    51421 aattaaggag tgtgttcctc aatgcgtaaa cctattatcg caggtaactg gaaaatgaac
    51481 aaaactgcat cagaagcaaa agaatttgct gaagcagtaa aaaacaaaat cccttcaaat
    51541 gacaaagttg attcagtcat cggatctcct gcattattct tacaagaatt aatgacatca
    51601 gctgaaggta cagaactaaa aatctcagca caaaactgct actgggaaaa ctcaggtgcg
    51661 ttcactggtg aaacttcacc agcagctctt gctgatctag gtgttcatta tgtgatcatc
    51721 ggacattcag aacgtcgtga atatttccat gaaacagacg aagacatcaa taaaaaagca
    51781 aaagctatct ttgcaaatgg tatgacacca atcctatgct gcggcgaatc tttagaaacc
    51841 tatgaagcag gcaaaactgc tgaatggatc gaaggacaaa ttacagctgg tttagctgat
    51901 ctttcagctg aacaagtgtc aaacatggtt attgcttatg aaccaatctg ggcaatcggt
    51961 actggtaaat cagctgacgc acaaatcgct gacgaaattt gtggagttgt acgtcaaaca
    52021 gttgaaaaac tttatggaaa agaagtatca gaagcagttc gtatccaata cggcggttct
    52081 gtaaaaccag aaaatattgc tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc
    52141 ggtggcgcaa gcttgcaagc agattcattt ttgtcattgt tagacgctgt aaaataattg
    52201 ttgaaaattt gcgaaaaatg caagaaatgt ttgcactaat gagcaaaatt aactaaaatc
    52261 gtatatgagg gaaatccctt aggaaaaaac aaactcaaag gagagacaaa acatgtcaat
    52321 tattactgat gtttacgctc gcgagatctt agactcacgt ggtaacccaa caatcgaagt
    52381 agaagtatac acagaaagcg gagcttttgg ccgcggaatg gttccttcag gtgcttcaac
    52441 tggtgaatac gaagcagttg aattacgtga cggagacaaa gctcgttatg gcggtaaagg
    52501 tgtaactaaa gctgtcgaca acgtaaacaa catcattgct gaagcaatca ttggctacga
    52561 tgttcgtgac caaatggcta tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa
    52621 aggtaaatta ggtgcgaacg ctattttagg tgtttctatc gctgttgcac gtgcagctgc
    52681 tgactaccta gaagtacctt tataccacta cctaggcgga ttcaacacaa aagtattgcc
    52741 aacaccaatg atgaacatca tcaacggcgg atcacatgct gataactcaa tcgacttcca
    52801 agaattcatg atcatgcctg ttggcgctcc aacattcaaa gaagctctac gtatgggtgc
    52861 tgaagtattc cacgcattag cttcaatctt gaaagctcgc ggattagcaa cttctgttgg
    52921 tgacgaaggt ggatttgctc caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat
    52981 cgaagcaatc gaaaaagctg gctatgtacc tggtaaagac gttgttcttg ctatggatgc
    53041 tgcttcttca gaattctacg acaaagaaaa aggtgtttac gtactagctg actcaggcga
    53101 aggcgaaaaa acaactgacg aaatgatcaa attctacgaa gaattagttt ctaaatatcc
    53161 aatcatctca atcgaagatg gattagacga aaacgactgg gacggattca agaaattaac
    53221 tgacgtatta ggcgacaaag ttcaattagt tggtgacgac ttgttcgtaa caaacactca
    53281 aaaattatct gaaggtatcg aaaaaggaat tgctaactca atccttatca aagtgaacca
    53341 aatcggtaca ttaacagaaa ctttcgaagc tatcgaaatg gctaaagaag ctggctacac
    53401 agcagttgta tctcaccgtt ctggtgaaac agaagattca acaatctctg atatcgctgt
    53461 tgcaacaaat gctggccaaa tcaaaactgg ttctctatca cgtactgacc gtattgctaa
    53521 atacaaccaa ttactacgta tcgaagacca acttggtgaa gttgcagaat acaaaggttt
    53581 gaaatctttc tacaacttaa aaaacaaata attcttttct aatgaagata catgatcgac
    53641 tattgaaaga tagtcgagta tgatcactta gttaagtaat cgaactctct acttgctgaa
    53701 atatcagcag gtagagagtt ttt
//
LOCUS       DAFGZE010000014        52349 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.014, whole genome shotgun sequence.
ACCESSION   DAFGZE010000014 DAFGZE010000000
VERSION     DAFGZE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.014"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            330..1175
                     /locus_tag="LTU54_000840"
     CDS             330..1175
                     /locus_tag="LTU54_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8295573.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="LTU54_000841"
     CDS             1456..2676
                     /locus_tag="LTU54_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HBL8295574.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="LTU54_000842"
     CDS             2673..5393
                     /locus_tag="LTU54_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295575.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="LTU54_000843"
     CDS             5377..6321
                     /locus_tag="LTU54_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL8295576.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="LTU54_000844"
     CDS             complement(6390..7400)
                     /locus_tag="LTU54_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8295577.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7445..8404)
                     /locus_tag="LTU54_000845"
     CDS             complement(7445..8404)
                     /locus_tag="LTU54_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8295578.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(8646..8990)
                     /locus_tag="LTU54_000846"
     CDS             complement(8646..8990)
                     /locus_tag="LTU54_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL8295579.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(8983..9417)
                     /locus_tag="LTU54_000847"
     CDS             complement(8983..9417)
                     /locus_tag="LTU54_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HBL8295580.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            9555..10292
                     /locus_tag="LTU54_000848"
     CDS             9555..10292
                     /locus_tag="LTU54_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295581.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            10285..11496
                     /locus_tag="LTU54_000849"
     CDS             10285..11496
                     /locus_tag="LTU54_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295582.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            11700..12482
                     /locus_tag="LTU54_000850"
     CDS             11700..12482
                     /locus_tag="LTU54_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HBL8295583.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            12552..13214
                     /gene="trmB"
                     /locus_tag="LTU54_000851"
     CDS             12552..13214
                     /gene="trmB"
                     /locus_tag="LTU54_000851"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBL8295584.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(13312..15066)
                     /gene="ade"
                     /locus_tag="LTU54_000852"
     CDS             complement(13312..15066)
                     /gene="ade"
                     /locus_tag="LTU54_000852"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBL8295585.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            15198..16127
                     /gene="tadA"
                     /locus_tag="LTU54_000853"
     CDS             15198..16127
                     /gene="tadA"
                     /locus_tag="LTU54_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBL8295586.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            16084..17133
                     /locus_tag="LTU54_000854"
     CDS             16084..17133
                     /locus_tag="LTU54_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HBL8295587.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            17130..17408
                     /locus_tag="LTU54_000855"
     CDS             17130..17408
                     /locus_tag="LTU54_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBL8295588.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            17383..17853
                     /locus_tag="LTU54_000856"
     CDS             17383..17853
                     /locus_tag="LTU54_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBL8295589.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            17870..18172
                     /locus_tag="LTU54_000857"
     CDS             17870..18172
                     /locus_tag="LTU54_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295590.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            18189..18560
                     /locus_tag="LTU54_000858"
     CDS             18189..18560
                     /locus_tag="LTU54_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HBL8295591.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            18532..18891
                     /locus_tag="LTU54_000859"
     CDS             18532..18891
                     /locus_tag="LTU54_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295592.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            19063..20070
                     /locus_tag="LTU54_000860"
     CDS             19063..20070
                     /locus_tag="LTU54_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8295593.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            20095..21279
                     /locus_tag="LTU54_000861"
     CDS             20095..21279
                     /locus_tag="LTU54_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBL8295594.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(21716..23299)
                     /locus_tag="LTU54_000862"
     CDS             complement(21716..23299)
                     /locus_tag="LTU54_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL8295595.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(23877..24020)
                     /locus_tag="LTU54_000863"
     CDS             complement(23877..24020)
                     /locus_tag="LTU54_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBL8295596.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(24242..24721)
                     /locus_tag="LTU54_000864"
     CDS             complement(24242..24721)
                     /locus_tag="LTU54_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL8295597.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(24794..25078)
                     /locus_tag="LTU54_000865"
     CDS             complement(24794..25078)
                     /locus_tag="LTU54_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295598.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(25156..25350)
                     /gene="ssrS"
                     /locus_tag="LTU54_000866"
     ncRNA           complement(25156..25350)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="LTU54_000866"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(25422..26708)
                     /locus_tag="LTU54_000867"
     CDS             complement(25422..26708)
                     /locus_tag="LTU54_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBL8295599.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            26846..27517
                     /locus_tag="LTU54_000868"
     CDS             26846..27517
                     /locus_tag="LTU54_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBL8295600.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            27483..27971
                     /locus_tag="LTU54_000869"
     CDS             27483..27971
                     /locus_tag="LTU54_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HBL8295601.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            27984..28931
                     /gene="prmA"
                     /locus_tag="LTU54_000870"
     CDS             27984..28931
                     /gene="prmA"
                     /locus_tag="LTU54_000870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBL8295602.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            28932..29696
                     /locus_tag="LTU54_000871"
     CDS             28932..29696
                     /locus_tag="LTU54_000871"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBL8295603.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            29929..32142
                     /locus_tag="LTU54_000872"
     CDS             29929..32142
                     /locus_tag="LTU54_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBL8295604.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            32153..32599
                     /locus_tag="LTU54_000873"
     CDS             32153..32599
                     /locus_tag="LTU54_000873"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBL8295605.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            32770..34029
                     /locus_tag="LTU54_000874"
     CDS             32770..34029
                     /locus_tag="LTU54_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HBL8295606.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(34118..35065)
                     /locus_tag="LTU54_000875"
     CDS             complement(34118..35065)
                     /locus_tag="LTU54_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL8295607.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            35459..36751
                     /locus_tag="LTU54_000876"
     CDS             35459..36751
                     /locus_tag="LTU54_000876"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBL8295608.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            36766..38535
                     /gene="aspS"
                     /locus_tag="LTU54_000877"
     CDS             36766..38535
                     /gene="aspS"
                     /locus_tag="LTU54_000877"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBL8295609.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            38644..39519
                     /locus_tag="LTU54_000878"
     CDS             38644..39519
                     /locus_tag="LTU54_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL8295610.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            39785..41668
                     /locus_tag="LTU54_000879"
     CDS             39785..41668
                     /locus_tag="LTU54_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL8295611.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(41743..42027)
                     /locus_tag="LTU54_000880"
     CDS             complement(41743..42027)
                     /locus_tag="LTU54_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL8295612.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(42017..42751)
                     /locus_tag="LTU54_000881"
     CDS             complement(42017..42751)
                     /locus_tag="LTU54_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBL8295613.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            42880..43503
                     /locus_tag="LTU54_000882"
     CDS             42880..43503
                     /locus_tag="LTU54_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBL8295614.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            43695..44816
                     /locus_tag="LTU54_000883"
     CDS             43695..44816
                     /locus_tag="LTU54_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HBL8295615.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            44823..47951
                     /locus_tag="LTU54_000884"
     CDS             44823..47951
                     /locus_tag="LTU54_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HBL8295616.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            47970..51164
                     /locus_tag="LTU54_000885"
     CDS             47970..51164
                     /locus_tag="LTU54_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HBL8295617.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(51310..52017)
                     /locus_tag="LTU54_000886"
     CDS             complement(51310..52017)
                     /locus_tag="LTU54_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL8295618.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        17073 a         9153 c        10833 g        15290 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta ttttttttcg
     7441 tttgtcaaat taagttagac aaatcatctt ttgtgacgta actcaaaaat acttccattg
     7501 gtgtccgata gtgtaatgat tttctaggaa tatgatttcg tttcgatgcc accgattgga
     7561 taaaggtttc agatacacca ttaaaatcca tagctttata caaaccatct ttacgtaata
     7621 agccatttga atgttcattc aatcctcgtt gagaaggtgt tcctggatcc gcaaaataaa
     7681 ttgaaatatc gttggtatta ctaatctttt tccaatttga gaattctttt ccacaatcaa
     7741 atgtgatgga tttaaataag ttttttggga cacaacgaaa ccattgattc agcctatttt
     7801 caatgtcttt agcctgtctg ccttcgggtt tcaaggtaat gatgacttta gatagccgtt
     7861 caactaatgt gatgaccgcg cttttatgtt ttccacccac gatagtgtca ccttccaggt
     7921 gaccaaattc atgatcaaac tgggtatagt ctgtctttct gtctcggatt gaccggcgaa
     7981 acccttgttt tcctcttttt tctttatgac cgtttggttt tctttttccc ttcattggta
     8041 aatcgtgagt gtcaaaggtt ttatctttaa acattcgata cagtgtgcgt accgaacaag
     8101 caataggaaa ctctgctcga ccaataagga catcaggagc ccacccttga acaacctttt
     8161 tttgaatgta ttcctgttgc tcatcaggta aaacaatcgg atgtcttccg cagttacgtt
     8221 tgtttttctt atattgttta tagtagctca ttgcgcttcc accagattta agaaaacgat
     8281 agaccttata aatagtttgt cgagaacgtt gtaacagatt ggctactttt gaaacaggtt
     8341 gtgattgatt gaaataagct tctatcatta cgagttcagt tggtgtaaga tggttatagg
     8401 tcatttgtac tcactcctta taattctttc gtcggaacta ttttgagtgt agcacaaatg
     8461 acttttttta gttgtctagc ttaattttac aatcggcgtt tatatattaa ctataccata
     8521 tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac
     8581 attcctagga aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat
     8641 cattattata tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca
     8701 ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt
     8761 gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt
     8821 ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct
     8881 tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca
     8941 gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac
     9001 ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg
     9061 attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg
     9121 tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt
     9181 catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc
     9241 tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa
     9301 tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata
     9361 tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata
     9421 aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat
     9481 tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg
     9541 gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc
     9601 ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac
     9661 tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa
     9721 aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac
     9781 aaattgggta tattccagag actccttctt tatatgaaga attgacattg agagaacata
     9841 tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa
     9901 agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa
     9961 agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata
    10021 tcatcgacga accattttta ggcttggatc cactagctat caatgcattg cttgaattga
    10081 tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg
    10141 cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt
    10201 ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata
    10261 ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat
    10321 caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt
    10381 cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca
    10441 ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt
    10501 aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa
    10561 atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg
    10621 atattggcat gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg
    10681 atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag
    10741 agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt
    10801 ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca
    10861 ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga
    10921 ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt
    10981 atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg
    11041 gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca
    11101 agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt
    11161 gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt
    11221 ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc
    11281 gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca
    11341 tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg
    11401 cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta
    11461 tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa
    11521 tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa
    11581 aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt
    11641 gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca
    11701 tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa
    11761 aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga
    11821 tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg
    11881 ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg
    11941 agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc
    12001 ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa
    12061 aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc
    12121 gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag
    12181 atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt
    12241 caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac
    12301 tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg
    12361 aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac
    12421 gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct
    12481 gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat
    12541 ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc
    12601 cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa
    12661 acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg
    12721 caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga
    12781 ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg
    12841 gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt
    12901 ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg
    12961 ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag
    13021 gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat
    13081 ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat
    13141 tttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa
    13201 agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat
    13261 ctcagaaaat gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt
    13321 atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa
    13381 tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa
    13441 aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa
    13501 ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac
    13561 acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag
    13621 attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc
    13681 atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa
    13741 tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc
    13801 gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac
    13861 tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa
    13921 cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga
    13981 atgatcagca aataaatggt cttggagttg cccagattga acaactaatg ttcccttata
    14041 gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata
    14101 cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc
    14161 catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc
    14221 gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct
    14281 tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc
    14341 ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt
    14401 agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat
    14461 accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag
    14521 gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac
    14581 ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc
    14641 aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct
    14701 tgcatcttct atcataaact caagacctgt actcccagca atgtttccta tttcatgggg
    14761 atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga
    14821 taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc
    14881 attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca
    14941 gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt
    15001 gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt
    15061 ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt cggcttttgc
    15121 caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt
    15181 ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt
    15241 ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt
    15301 ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac
    15361 tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt
    15421 taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag
    15481 gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac
    15541 cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg
    15601 gattatacct tttcagcgga ccagtcggat caggcaagac ttctctaatg tatcggttgg
    15661 ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt
    15721 tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgaaac
    15781 tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg
    15841 cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac
    15901 gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat
    15961 ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt
    16021 ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa
    16081 gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga
    16141 agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg
    16201 caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat
    16261 atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat
    16321 ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact
    16381 ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag
    16441 ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg
    16501 ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt
    16561 cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg
    16621 gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata
    16681 agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta
    16741 gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag
    16801 gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag
    16861 gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt
    16921 aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc
    16981 gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca
    17041 atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct
    17101 gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg
    17161 gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt
    17221 tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca
    17281 tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta
    17341 ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc
    17401 ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt
    17461 ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa
    17521 ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc
    17581 gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt
    17641 tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt
    17701 accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta
    17761 aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata
    17821 gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct
    17881 tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt
    17941 gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga
    18001 tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata
    18061 cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa
    18121 agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa
    18181 ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca
    18241 tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac
    18301 aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact
    18361 acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa
    18421 gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa
    18481 aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat
    18541 ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt
    18601 ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg
    18661 gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta
    18721 tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct
    18781 gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc
    18841 tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt
    18901 cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt
    18961 cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca
    19021 ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg
    19081 gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact
    19141 tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt
    19201 gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag
    19261 ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg
    19321 aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt
    19381 tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt
    19441 ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat
    19501 gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca
    19561 gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag
    19621 gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg
    19681 aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat
    19741 catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg
    19801 atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa
    19861 gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg
    19921 caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc
    19981 ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag
    20041 tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct
    20101 aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca
    20161 gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt
    20221 accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa
    20281 gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat
    20341 gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta
    20401 gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat
    20461 aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt
    20521 gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc
    20581 ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt
    20641 catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa
    20701 atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc
    20761 atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt
    20821 gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa
    20881 atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat
    20941 atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc
    21001 gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca
    21061 attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa
    21121 ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa
    21181 gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa
    21241 ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata
    21301 aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc
    21361 aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg
    21421 cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat
    21481 cccaatattt tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac
    21541 atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa
    21601 accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc
    21661 ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt
    21721 caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt
    21781 ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt
    21841 cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat
    21901 ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt
    21961 caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg
    22021 ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac
    22081 ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac
    22141 ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg
    22201 attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac
    22261 tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa
    22321 cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg
    22381 gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga
    22441 aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc
    22501 gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt
    22561 tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt
    22621 cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg
    22681 atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat
    22741 tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga
    22801 ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacgg tgtggaatcc
    22861 ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa
    22921 agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg
    22981 tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat
    23041 ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga
    23101 tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga
    23161 tttgtaaaaa ggttgatttg ccagttccgt tgataccgac taaggcaatt ttttcaccag
    23221 cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag
    23281 aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc
    23341 cctggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg
    23401 attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc
    23461 aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt
    23521 catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag
    23581 tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc
    23641 agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga
    23701 gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac
    23761 ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca
    23821 ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa
    23881 ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa
    23941 aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca
    24001 gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt
    24061 aaaccacacc agggcgacta atcgccctgg tgttcacttt ttttatttta cttgaactca
    24121 ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt
    24181 tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt
    24241 gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa
    24301 gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt
    24361 ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga
    24421 tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt acattattca
    24481 agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag
    24541 cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga
    24601 catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag
    24661 ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca
    24721 taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga
    24781 aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg
    24841 tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa
    24901 aagctgttaa aatacccatt acgcctaaaa cagcatatgc agcaccgtaa aattgtagga
    24961 atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata
    25021 aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga
    25081 acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt
    25141 attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg
    25201 cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt
    25261 taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca
    25321 aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa
    25381 gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc
    25441 tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta
    25501 tcttttggct gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc
    25561 caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca
    25621 cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata
    25681 tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga
    25741 tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct
    25801 aatacagcgt tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa
    25861 ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg
    25921 agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata
    25981 acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct
    26041 tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta
    26101 gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga
    26161 agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt
    26221 aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc
    26281 tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg
    26341 atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct
    26401 aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct
    26461 acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta
    26521 gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga
    26581 atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact
    26641 agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc
    26701 tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca
    26761 agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt
    26821 gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg
    26881 gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac
    26941 accagaagga agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga
    27001 cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa
    27061 accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca
    27121 gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga
    27181 tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa
    27241 attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc
    27301 gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat
    27361 cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa
    27421 tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga
    27481 aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag
    27541 actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct
    27601 tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag
    27661 aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg
    27721 atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag
    27781 tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc
    27841 ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcaac
    27901 aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc
    27961 caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg
    28021 aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg
    28081 aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa
    28141 atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt
    28201 tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc
    28261 tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag
    28321 cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct
    28381 gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg
    28441 catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct
    28501 tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag
    28561 caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt
    28621 cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg
    28681 atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata
    28741 tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg
    28801 tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt
    28861 ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag
    28921 aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg
    28981 ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg
    29041 aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg
    29101 aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt
    29161 cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat
    29221 ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat
    29281 gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag
    29341 cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt
    29401 tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc
    29461 aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct
    29521 tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac
    29581 acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt
    29641 atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt
    29701 cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa
    29761 tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg
    29821 aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat
    29881 ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa
    29941 gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat
    30001 gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga
    30061 aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta
    30121 aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa
    30181 atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc
    30241 gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac
    30301 cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat
    30361 agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct
    30421 caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa
    30481 tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc
    30541 cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat
    30601 attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac
    30661 atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac
    30721 ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca
    30781 atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa
    30841 gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa
    30901 attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg
    30961 gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc
    31021 tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa
    31081 ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt
    31141 actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc
    31201 ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg
    31261 aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac
    31321 tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc
    31381 caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat
    31441 ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt
    31501 tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc
    31561 ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa
    31621 aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta
    31681 cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc
    31741 tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata
    31801 tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt
    31861 ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg
    31921 gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg
    31981 ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc
    32041 catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa
    32101 gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc
    32161 agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat
    32221 tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga
    32281 ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa
    32341 tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc
    32401 ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat
    32461 tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg
    32521 acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat
    32581 tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg
    32641 cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct
    32701 tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg
    32761 ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg
    32821 tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc
    32881 aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta
    32941 ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca
    33001 ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat
    33061 atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag
    33121 aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag
    33181 atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa
    33241 gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct
    33301 ccgattgatc acataaaagg tgtcgagaat cgcaaaaaat cagaaaaatg ggcgcaaggc
    33361 ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct
    33421 tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa
    33481 ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat
    33541 gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa
    33601 gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt
    33661 tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta
    33721 gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc
    33781 aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca
    33841 caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat
    33901 agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa
    33961 gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac
    34021 aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc
    34081 ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat
    34141 caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata
    34201 cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc
    34261 aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat
    34321 cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag
    34381 gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg
    34441 aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt
    34501 ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat
    34561 ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt
    34621 ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat
    34681 ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt
    34741 ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat
    34801 gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct
    34861 cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga
    34921 tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa
    34981 aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg
    35041 aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa
    35101 ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact
    35161 ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag
    35221 tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc
    35281 ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta
    35341 agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg
    35401 ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat
    35461 gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca
    35521 gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac
    35581 accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt
    35641 ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga
    35701 aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc
    35761 gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg
    35821 acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac
    35881 tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat
    35941 ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt
    36001 gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca
    36061 tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga
    36121 agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt
    36181 ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact
    36241 agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca
    36301 agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc
    36361 aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga
    36421 ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga
    36481 aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga
    36541 ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc
    36601 caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc
    36661 aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga
    36721 agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg
    36781 atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg
    36841 gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga
    36901 atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa
    36961 tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca
    37021 gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg
    37081 aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata
    37141 cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta
    37201 agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc
    37261 gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca
    37321 cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg
    37381 ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat
    37441 ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca
    37501 gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc
    37561 ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac
    37621 ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc
    37681 aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca
    37741 ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa
    37801 tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg
    37861 aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat
    37921 gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca
    37981 ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc
    38041 aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat
    38101 gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa
    38161 gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc
    38221 ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc
    38281 tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga
    38341 ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga
    38401 gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg
    38461 agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg
    38521 aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac
    38581 aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt
    38641 tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta
    38701 tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat
    38761 atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg
    38821 ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta
    38881 ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg
    38941 acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt
    39001 gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta
    39061 tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt
    39121 ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc
    39181 tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc
    39241 gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta
    39301 gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt
    39361 gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct
    39421 ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac
    39481 gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca
    39541 tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat
    39601 cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt
    39661 atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt
    39721 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
    39781 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
    39841 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
    39901 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
    39961 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
    40021 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
    40081 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
    40141 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
    40201 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
    40261 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
    40321 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
    40381 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
    40441 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
    40501 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
    40561 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
    40621 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
    40681 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
    40741 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
    40801 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
    40861 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
    40921 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
    40981 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
    41041 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
    41101 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
    41161 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
    41221 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
    41281 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact
    41341 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
    41401 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
    41461 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
    41521 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
    41581 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
    41641 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
    41701 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
    41761 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
    41821 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
    41881 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
    41941 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
    42001 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
    42061 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
    42121 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
    42181 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
    42241 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
    42301 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
    42361 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
    42421 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
    42481 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
    42541 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
    42601 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
    42661 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
    42721 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
    42781 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
    42841 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
    42901 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
    42961 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
    43021 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
    43081 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
    43141 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
    43201 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
    43261 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
    43321 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
    43381 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
    43441 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
    43501 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
    43561 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
    43621 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
    43681 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
    43741 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
    43801 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
    43861 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
    43921 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
    43981 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
    44041 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
    44101 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
    44161 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
    44221 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
    44281 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
    44341 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
    44401 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
    44461 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
    44521 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
    44581 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
    44641 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
    44701 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
    44761 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
    44821 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
    44881 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
    44941 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
    45001 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
    45061 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
    45121 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
    45181 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
    45241 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
    45301 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
    45361 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
    45421 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
    45481 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
    45541 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
    45601 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
    45661 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
    45721 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
    45781 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
    45841 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
    45901 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
    45961 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
    46021 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
    46081 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
    46141 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
    46201 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
    46261 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
    46321 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
    46381 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
    46441 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
    46501 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
    46561 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
    46621 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
    46681 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgcaagaata gagctggaag
    46741 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
    46801 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
    46861 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
    46921 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
    46981 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
    47041 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
    47101 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
    47161 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
    47221 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
    47281 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
    47341 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
    47401 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
    47461 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
    47521 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
    47581 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
    47641 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
    47701 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
    47761 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
    47821 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
    47881 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
    47941 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
    48001 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
    48061 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
    48121 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
    48181 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
    48241 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
    48301 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
    48361 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
    48421 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
    48481 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
    48541 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
    48601 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
    48661 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
    48721 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tccgatcaaa
    48781 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
    48841 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
    48901 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
    48961 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
    49021 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
    49081 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
    49141 tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
    49201 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
    49261 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
    49321 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
    49381 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
    49441 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
    49501 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
    49561 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
    49621 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
    49681 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    49741 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    49801 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    49861 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    49921 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    49981 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    50041 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    50101 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    50161 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    50221 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    50281 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    50341 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    50401 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    50461 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    50521 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    50581 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    50641 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    50701 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    50761 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    50821 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    50881 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    50941 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    51001 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    51061 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    51121 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    51181 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    51241 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    51301 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    51361 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    51421 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    51481 ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg
    51541 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    51601 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    51661 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    51721 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    51781 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    51841 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    51901 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    51961 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    52021 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    52081 aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac
    52141 agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt
    52201 ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg
    52261 tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa
    52321 ataacgttat ttgatttatt tgtttatag
//
LOCUS       DAFGZE010000015        50110 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.015, whole genome shotgun sequence.
ACCESSION   DAFGZE010000015 DAFGZE010000000
VERSION     DAFGZE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.015"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..305)
                     /locus_tag="LTU54_000887"
     CDS             complement(<1..305)
                     /locus_tag="LTU54_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL8295619.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKS"
     gene            complement(307..504)
                     /locus_tag="LTU54_000888"
     CDS             complement(307..504)
                     /locus_tag="LTU54_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HBL8295620.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(898..2625)
                     /locus_tag="LTU54_000889"
     CDS             complement(898..2625)
                     /locus_tag="LTU54_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HBL8295621.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2618..3811)
                     /locus_tag="LTU54_000890"
     CDS             complement(2618..3811)
                     /locus_tag="LTU54_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HBL8295622.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3998..4642)
                     /locus_tag="LTU54_000891"
     CDS             complement(3998..4642)
                     /locus_tag="LTU54_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8295623.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4736..4870)
                     /locus_tag="LTU54_000892"
     CDS             complement(4736..4870)
                     /locus_tag="LTU54_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBL8295624.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4872..7004)
                     /gene="feoB"
                     /locus_tag="LTU54_000893"
     CDS             complement(4872..7004)
                     /gene="feoB"
                     /locus_tag="LTU54_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBL8295625.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7001..7474)
                     /locus_tag="LTU54_000894"
     CDS             complement(7001..7474)
                     /locus_tag="LTU54_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBL8295626.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7875..8099
                     /gene="nrdH"
                     /locus_tag="LTU54_000895"
     CDS             7875..8099
                     /gene="nrdH"
                     /locus_tag="LTU54_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBL8295627.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8258..10417
                     /gene="nrdE"
                     /locus_tag="LTU54_000896"
     CDS             8258..10417
                     /gene="nrdE"
                     /locus_tag="LTU54_000896"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBL8295628.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10467..11432
                     /gene="nrdF"
                     /locus_tag="LTU54_000897"
     CDS             10467..11432
                     /gene="nrdF"
                     /locus_tag="LTU54_000897"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBL8295629.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11521..12660)
                     /locus_tag="LTU54_000898"
     CDS             complement(11521..12660)
                     /locus_tag="LTU54_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL8295630.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12969..13682)
                     /locus_tag="LTU54_000899"
     CDS             complement(12969..13682)
                     /locus_tag="LTU54_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBL8295631.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13936..14637)
                     /gene="nagB"
                     /locus_tag="LTU54_000900"
     CDS             complement(13936..14637)
                     /gene="nagB"
                     /locus_tag="LTU54_000900"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBL8295632.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14871..16403
                     /locus_tag="LTU54_000901"
     CDS             14871..16403
                     /locus_tag="LTU54_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL8295633.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16594..17796)
                     /locus_tag="LTU54_000902"
     CDS             complement(16594..17796)
                     /locus_tag="LTU54_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8295634.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17878..18567)
                     /locus_tag="LTU54_000903"
     CDS             complement(17878..18567)
                     /locus_tag="LTU54_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL8295635.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18673..19041)
                     /gene="rplL"
                     /locus_tag="LTU54_000904"
     CDS             complement(18673..19041)
                     /gene="rplL"
                     /locus_tag="LTU54_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBL8295636.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19094..19597)
                     /locus_tag="LTU54_000905"
     CDS             complement(19094..19597)
                     /locus_tag="LTU54_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBL8295637.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19801..19928)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(20007..20696)
                     /gene="rplA"
                     /locus_tag="LTU54_000906"
     CDS             complement(20007..20696)
                     /gene="rplA"
                     /locus_tag="LTU54_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBL8295638.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20800..21222)
                     /gene="rplK"
                     /locus_tag="LTU54_000907"
     CDS             complement(20800..21222)
                     /gene="rplK"
                     /locus_tag="LTU54_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBL8295639.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21565..22233
                     /gene="sdaAB"
                     /locus_tag="LTU54_000908"
     CDS             21565..22233
                     /gene="sdaAB"
                     /locus_tag="LTU54_000908"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HBL8295640.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22246..23118
                     /gene="sdaAA"
                     /locus_tag="LTU54_000909"
     CDS             22246..23118
                     /gene="sdaAA"
                     /locus_tag="LTU54_000909"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HBL8295641.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23209..24228)
                     /locus_tag="LTU54_000910"
     CDS             complement(23209..24228)
                     /locus_tag="LTU54_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL8295642.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24401..25306)
                     /locus_tag="LTU54_000911"
     CDS             complement(24401..25306)
                     /locus_tag="LTU54_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL8295643.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25365..25910)
                     /gene="nusG"
                     /locus_tag="LTU54_000912"
     CDS             complement(25365..25910)
                     /gene="nusG"
                     /locus_tag="LTU54_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBL8295644.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26011..26181)
                     /gene="secE"
                     /locus_tag="LTU54_000913"
     CDS             complement(26011..26181)
                     /gene="secE"
                     /locus_tag="LTU54_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBL8295645.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26205..26357)
                     /gene="rpmG"
                     /locus_tag="LTU54_000914"
     CDS             complement(26205..26357)
                     /gene="rpmG"
                     /locus_tag="LTU54_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL8295646.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26519..27163
                     /locus_tag="LTU54_000915"
     CDS             26519..27163
                     /locus_tag="LTU54_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL8295647.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27219..27722)
                     /locus_tag="LTU54_000916"
     CDS             complement(27219..27722)
                     /locus_tag="LTU54_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL8295648.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27735..27781)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28319..29284
                     /locus_tag="LTU54_000917"
     CDS             28319..29284
                     /locus_tag="LTU54_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL8295649.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29383..30555)
                     /locus_tag="LTU54_000918"
     CDS             complement(29383..30555)
                     /locus_tag="LTU54_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295650.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30542..31687)
                     /locus_tag="LTU54_000919"
     CDS             complement(30542..31687)
                     /locus_tag="LTU54_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295651.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(31701..32625)
                     /locus_tag="LTU54_000920"
                     /pseudo
     CDS             complement(31701..32625)
                     /locus_tag="LTU54_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            32742..33821
                     /locus_tag="LTU54_000921"
     CDS             32742..33821
                     /locus_tag="LTU54_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL8295652.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            33853..34446
                     /locus_tag="LTU54_000922"
     CDS             33853..34446
                     /locus_tag="LTU54_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295653.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(34556..34783)
                     /locus_tag="LTU54_000923"
     CDS             complement(34556..34783)
                     /locus_tag="LTU54_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL8295654.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(34886..37474)
                     /locus_tag="LTU54_000924"
     CDS             complement(34886..37474)
                     /locus_tag="LTU54_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBL8295655.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(37494..38153)
                     /locus_tag="LTU54_000925"
     CDS             complement(37494..38153)
                     /locus_tag="LTU54_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL8295656.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(38202..38705)
                     /gene="trmL"
                     /locus_tag="LTU54_000926"
     CDS             complement(38202..38705)
                     /gene="trmL"
                     /locus_tag="LTU54_000926"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBL8295657.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(38926..39792)
                     /locus_tag="LTU54_000927"
     CDS             complement(38926..39792)
                     /locus_tag="LTU54_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL8295658.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            39901..40542
                     /locus_tag="LTU54_000928"
     CDS             39901..40542
                     /locus_tag="LTU54_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBL8295659.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(40604..41965)
                     /gene="mgtE"
                     /locus_tag="LTU54_000929"
     CDS             complement(40604..41965)
                     /gene="mgtE"
                     /locus_tag="LTU54_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBL8295660.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(41983..42882)
                     /locus_tag="LTU54_000930"
     CDS             complement(41983..42882)
                     /locus_tag="LTU54_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBL8295661.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(42884..43681)
                     /locus_tag="LTU54_000931"
     CDS             complement(42884..43681)
                     /locus_tag="LTU54_000931"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HBL8295662.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(43659..44363)
                     /locus_tag="LTU54_000932"
     CDS             complement(43659..44363)
                     /locus_tag="LTU54_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBL8295663.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            44494..45069
                     /locus_tag="LTU54_000933"
     CDS             44494..45069
                     /locus_tag="LTU54_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HBL8295664.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            45187..45864
                     /locus_tag="LTU54_000934"
     CDS             45187..45864
                     /locus_tag="LTU54_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HBL8295665.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(45943..47751)
                     /gene="pepF"
                     /locus_tag="LTU54_000935"
     CDS             complement(45943..47751)
                     /gene="pepF"
                     /locus_tag="LTU54_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBL8295666.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSDAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(47927..48925)
                     /locus_tag="LTU54_000936"
     CDS             complement(47927..48925)
                     /locus_tag="LTU54_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HBL8295667.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(49151..49804)
                     /locus_tag="LTU54_000937"
     CDS             complement(49151..49804)
                     /locus_tag="LTU54_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HBL8295668.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT        15003 a        10509 c         8783 g        15815 t
ORIGIN      
        1 tgagatttat ctccataatc tgctcgatat aaaccattta ttccagcagt tcttccaatt
       61 tcactcaaat ttcttattaa ttcctcttga tattcagtga atcttatttt attagtataa
      121 gcagtaaaat atagataatt caccaatcta tttattaaag gaccgggatc taattcatat
      181 ttttgttttt ctaattttaa atataccttt tgtaaaacct caagaatatc agaatattta
      241 ggatctttac ttatagatat atcattatat aattcatgaa caatttgctt agcatttttt
      301 ttcattttag cacttccctg gaattgctcc acctaaaaaa ccacctatag acattcctgc
      361 aatacaagta gttgccttgg cccaatcgac cgtacattta tttttagtgc aatacactcc
      421 atttccataa tattttccac tatgagtggt acccccatag ataagttgtg tctctttaat
      481 agacaaaatt tttaaatgtt tcataatcta atcaccccta aattaatgtt tttagactat
      541 tattctaaca ttcattaaat ttcaaaaaac aattccttct tgaaacgtag caaaataatt
      601 cctgaataag aatttaatca cttttaagta tataaactat gtatattctt taatattttt
      661 tgagaatgca cttcgtacat aataaacttt aatcacatta gtctttcata cctacgatcg
      721 atctctttga ctcgcaccca taaattcata atagtttgtc ggcgtctttg gacgtttcga
      781 agatgagcta cgcattctct ttttttatag acaaaaaaga tagagacgaa catggagctt
      841 ccaaatttgg atatttgatc caattttgga agctccattt atttcgtgtc tgtcttttta
      901 ttttttccag ctttcgacta attcttggtt ctctttgatc cagtcttttg ctgcttgctg
      961 tggatctttt ccattgttga tttccaacat aactttttcc atatcttttt ctgaccaatg
     1021 gaaattgtct aatactttgt aagcttctgg ctggtcttct ttcaatcctt ttcgaaccat
     1081 cgtatggatc tgctcttcgc ttcccattcc gttttcagga tcttctaagt atttcagatc
     1141 atatttggca aacatccaat gaggtgtcca tcctgtaact acaattggtt cgtgtttttt
     1201 gatcgcttga cctagtgcaa cggtcattgc tcctgaagaa gatgtctcta ctttccagtc
     1261 tttcaagttg tcatattttt ggatcgtgtt ttcagcagcc gtaactacac cagctcctgg
     1321 ctcgattcct atgattttct tacctgcctg atcggttaac tcatcgatcg aatttacatc
     1381 catgtaggct ggcactgcta aaccaacttt tgctcctgtc aaattggcac ctaggtcttc
     1441 gacttgatct ttatattgtg catattgtga accatgagtt ttaggtagcc aagctgaaac
     1501 catggcatct gactcgccat ttgaaacaga tttccacatg attgaattat ctaatggcgt
     1561 catcgttaca tcatagccca tttgtttcaa tacttcaccg actacatttg ttgaagcaac
     1621 ttctgtatcc cattcaacat aagataagtt gatacgtttt gtttctttgg cagatgaaaa
     1681 actccctata ccaatcgcac ctaagatcac tactactaca gcaccaatga tcagtccttg
     1741 acgtttttta cttttctgtg ttgttgctcc tgcagctttt ttattgtttg gctggttcaa
     1801 atgctgtgta aaacggtcaa tgatgattgc taagataact aatgcaaccc cgttgacaaa
     1861 tccattcccg acttgtgctc tttgcaatgc tgataatacg ccgcgtccta agccgggtgc
     1921 accgatcata gaggcaataa cgaccattga aagtgacagc attgttgttt gattgatccc
     1981 tgccatgatc gtacttttag ctaaaggcaa ttctagtttg aatagttttt gacgacttgt
     2041 gctcccgaat gaatcagaag cttctactaa ttctttagga acttgtcgaa tccctaaatt
     2101 cgtaaaacga acggttggtg gcagagcaaa gatgactgag gcaaataccc ctggaaccat
     2161 cccgataccg aaaaatgcaa ctgctggaat caaataaacg aatcctggca ttgtctgcat
     2221 aaagtcaagg atcggtttga taatactttg ggctttactg ctttttgcca tcaagatccc
     2281 taatggtacc ccaatgatga tcgagatcac actagataaa agaacaagtg tgacagtatt
     2341 catcaaatca ttccatagac cttggttata aataaaccat agaccgatta atgtgaacaa
     2401 cgtaagtcct ggacgtttct tagaaataaa gaaagctgct accgtcaata acaagataaa
     2461 caaaggagct ggaatcacta acagtaaatt tgtaatcccc gacatcactg cctgtccaat
     2521 cgtttgtaaa aatgaaaata ggccagaaaa agttgtcgta aaccattctg tgatattttc
     2581 tacccagttt gccactggta attgatagtt caatagatta ttcatgttca ctcacctcgc
     2641 ttgtttctgc taatgcttca atcacactac ctcggatgac gacacctttt aattttccat
     2701 cctctacaac tgcaagtgga gtcggtgagt cataaataat attgaaaata tcacttacca
     2761 ccatgtcttt cccaatcacc gtcacatttt catctaggaa gtcgatcaat ggacgttttt
     2821 gcttcctagc ttctaacgct tgatctgcag tgatgatccc tttcaggtgt ctctttctgt
     2881 caacagccat cagcatgctg acttcttcat tacgcatcct tgtcaatgca acgtttggtc
     2941 catcagattc gatattcgtc gtcaatgcag gtaccatgat gttttgagcc gtaagcactt
     3001 tggaacgatc gacttcttcc acaaattcac gtacataatc atttgctgga ttggtcaata
     3061 tttcttcacc tgttccgatc tgcatgattt cgccgtcttt catcaaggcg attctgtccc
     3121 caatccgtaa agcttcattc aaatcatggg taatgaaaat aatcgttttt tgaacattgg
     3181 cttgcaggtc cagtaattcg tcttgcattt ctcgccggat cagcggatca agagccgaga
     3241 acgcttcgtc catcagcaag atctcaggat catttgctaa agctctggct aggccaaccc
     3301 gttgttgcat cccaccagat aactgactag gatattggtc tttaaatgaa agcaaactag
     3361 aattttctaa tgctttttct gctttttctt gtcgttcttc tttaggtaca ccacggactt
     3421 ctaacccgta ttctgtattt tccaagatcg ttcgatgtgg aaacagaccg aagttttgga
     3481 aaaccatatt gattttatga cgacggactt ctcgcaattc ttctttgctt aacttcgata
     3541 catcttctcc atcaatatag atactgcccg aagttggctc aatcagacga ttcaatagac
     3601 ggatcaatgt ggatttccca ctcccggaaa gccccatgat cacgaagatt tctccttctt
     3661 ttacgtcaaa attcacgtca tacacaccaa ccgttgcacc ggttttcttt aaaatctccg
     3721 ttttcggctg gtgatcattc atcatatcta aagcggcttg tgtcttttta ccaaatatct
     3781 ttgttaaatg ttttacttct acttttacca aacaaattct ccttttttat gtaaattcat
     3841 ttttctttct gaaaaaagtg accacactat gttaacatca tgtagtcact ttgtcaaaaa
     3901 tgaaagaaat ttgcttgtcc aaactgaaaa aaacttggct aaatctttgt aaataaaagg
     3961 tttagccaag ttaatatttt tttaatattt cacacaatca ttctgcaagc tgatgctccg
     4021 ggtaaaaaaa gtttgtcata cgctgtagtg tatattcatt tccaataaaa tgaacgatgt
     4081 cccctgccgt aaatttagca taggggccag gtgaaacaag cagttcttca ttatgagaaa
     4141 ttgcgataac tgtggcgcca gtcgcctgcc agatattcaa ctcgctaatt gaacggtcca
     4201 gatgcattgc tttttctgat aaagataatt caaacggaag taaaggattc atcttatgta
     4261 ttcgcttcgt ttgattgacc agctgatcca ataattgaga aaaatgattc agctcatctt
     4321 gttgcttctc cacacttgcc atgatttctg cttttgtatc ttggatggat tgtacatctt
     4381 gatattgcaa taggaaatct tttgcttttt ccttcgaagc aacaaatgcc ccacttccat
     4441 gccgtacttc catgattcca agatctacca gcacattgat agccttgcgg gtagtctcag
     4501 gagaagcatt gtacgtactg gcaagtgttg agcgggcatg gatcttctca cccactttat
     4561 atcggttttc aacgatacgt tcggctaaat caacagcaat ctgctggtag cgcgggcgga
     4621 cgattttttt ctttccagac attttctttc ctctttcttt ttttactttc cctcatctta
     4681 ctatgctcta gcaaaataag caaaaaaggg gagaatagcc tcttgcaacg actgatcatc
     4741 gcttgatctg ttcctttttt actgggcaag ctgtttgaca gtcatcacaa ttcttaccgg
     4801 attttaaacg agtatagaca attgcgcctg cacttccaaa gattaacaca cttaataaga
     4861 atgtcgccat cctattcctc tccttctaat gacaatatag tataaaactg gttcttggat
     4921 tttggctgac ggaacaaaac ataaacgagt cctatcatca gtaaaatagc aagatacgtc
     4981 cctaacgcta cctgtccacc ctccaataag tgtcccaact gataaatcac aaaactaact
     5041 aaataagcta aaccgcattg atagcctatg gcgatccaag tccatttctt ggcattcatt
     5101 tcccgatgga tagccccaat tgccgcaaaa caaggcgcac aaagtaaatt gaaggctaac
     5161 aatgaataag ctgctgcggg agtatatgtg tgttgtagca cttgccagat ttctctgcca
     5221 ttttcggata cttccgcatg accaaacaaa ataccaaatg tgccaataat attttcttta
     5281 gcgaccaatc ctgtaatcgc tgcgactgtt ccttgccagt ttccccaacc aagcggctga
     5341 aaaacaactg ccaggattcg cccgaaaaag gctaagatac tttggtcttc agaaacaggt
     5401 tgagcataaa aattataaga agagctgaac cagatcacga tactagaaac aaagataatc
     5461 gttcctgctt ttttaacaaa agacttgctg cggtcatagg tttgtcttag gaccgctgac
     5521 cattgaggaa aatgatatgc gggcaattcc atgataaaag gagctgtatc tccagcaaac
     5581 aaacgtgttt ttttcaagct gattccagag aaaataattg cagtcatacc aagaaaatag
     5641 gcagaaggag ctacccaaga ttgatgagga aaaaaggccc ctgctactaa ggcaataatc
     5701 ggcaactttg cagaacaagg catgaaggtt gtgaccatga tcgtcaaacg acgatcttgc
     5761 tcgttttcaa tcgtgcgact ggccatgacg cctggcactc cacatcctgt agcaatcagc
     5821 ataggaatga aggattttcc tgaaagaccg aatttcctga aaagtcgatc catgacaaaa
     5881 gcgatcctcg ccatatatcc gcagtcttcc agaaagctca agcataaaaa caatataatc
     5941 aattgcggta gaaatcctaa cactgctccc acgcctgcga tgatgccgtt caagatcagc
     6001 tcctgcatcc atcccgctac ttgccattca gctaacaatc tgccaacatg ttctggtaac
     6061 catgtaccaa acaatgtgtc attgatccaa tctgtcccca ttgttcctac tgtttgaata
     6121 gccagataat agatcagcca catcacaaat gcgaagatcg gcaaggctaa ccaacgattc
     6181 gttgcaatct gatcgatttt gtcactcatc gataattgga aagaatcatt tgacacaaca
     6241 cagagtgtac atagttgggt aataaattca taacgttcat tgaccagaat cgtctcacta
     6301 tcgtcgtcca atagtttctc tgttaactgg atcagttgct cgatctcttt ttgctgagaa
     6361 actgctatct cgtaatcctc tcttaccttc tgatctcgtt caaaatattt cattgcatac
     6421 cagcgtttct tgccgctttg ttctcctagt acatcttcaa tttcatgtaa agctgcttct
     6481 aaacgtggat catatgtaca gggaacagga atagaaggcc gcttttttgc agactcctga
     6541 atcttttcga ttagtttttc gattccttgc tttttcaata cgctgattgg taaaactggt
     6601 acaccaagac cataggaaag cttttctaga ttgatttttt ttccttcttt tttgatgaca
     6661 tccatcatat tcagaccaat gatcatagga atgtctaaat ccaataactg cgtagttaaa
     6721 taaagatttc gttctaaatt ggtagcatca ataatgttga tgatcagttc aggctgttcc
     6781 tgaaccaaat aatctcttgc aatcacttct tctggtgtat aaggagacaa agaatagatg
     6841 ccgggtaggt cctgaatatg aatgttcgga ttttttttca acgatccttc ttttttttct
     6901 actgttacgc ccggccaatt ccctacatat tgattcgaac cagtcaagag attgaacaag
     6961 ctagttttcc cgctgtttgg atttccgact aacgcgatat tcattctttt tcctcccttt
     7021 gtatcaagat tagttcggcc tctgactttc gtaacgtcag ttcataccct cgcactgtca
     7081 gttcgattgg atctcccaga ggggctagtt ttcggataac tatttttaca tttcgcgtca
     7141 atcccatatc cattaaccgt cgcttcactg cgccttgtcc gtggattgct acgacgatcc
     7201 cagtctctcc tgcttttaat tgatcaagag aagtccagct ttcagaggaa gcagtctttt
     7261 tattgacaaa gatttgacct agtatttccc gacttactgc cactcgatga ctttttacca
     7321 gtaaaatggc attgttgcct gataaattga cgacgacgat tttacttccg gcaacgatcc
     7381 cgatattttg caaatggcgt tttgtttttt cttctgtttc gattcgttcg acttgataaa
     7441 cgaaattctg ctttgcctgc gctaaggtct gcatattgat tcctctcttg tgaaataaat
     7501 catgtattaa aatcattcat tatatgttct aattttctta cgtgttacgc aaataaatga
     7561 gaatcattct cacatagaat actctcggta attgagaatg tcaattatac ttgtgatatt
     7621 atgagcgaat ctttagtatt gatagataaa aatgagaaaa aaatatttct tcatagtttt
     7681 ccacaatttt ttctctatta aatttgctat tttcccacac gatacttgtt ttcagctctt
     7741 ttttcaaaaa cacaaaactt agtattccac agtttccaca acacaatatg tagtgtttgt
     7801 tgtttgactt ttttactaag agttttatac tatctattat agataataag aatgtgttag
     7861 aggaggcact caccatgaac gtaaaaatct tttctaaaaa taattgtatg caatgtaaga
     7921 tggtcaaacg gtttttaaca gaaaatcaaa ttgcttttga agaagtcaat atcgatgaac
     7981 agcctgaagc agtagactgg ttgaaagcac aaggattcca aagtgttcct gtcattactt
     8041 ctgatgcaga aacagttatc ggattccgtc ctgatcagtt gcgtaaactt gccagctaac
     8101 atgactaaaa gaatcaaaac gtgatacatg gatttgaagg gcttgagaca gcaccagttt
     8161 ttgtcgcaaa cttccaaatc cttttttaaa gcttaaaaac aatgcaatga tttgcaatca
     8221 agcatcatca aatacgaaat gaaggttggg cgtacgtatg agtcttaaaa atttacaaga
     8281 tgtcagttat ttcaagctga ataatgaaat caatcggcca gtcaatggtc aaatcccttt
     8341 gaataaggat aaagaagctt tagttgcctt ttttgaggaa aatgtcaaac caaatacgat
     8401 gacttttcta tctattatgg aaaaaatcaa ttacttgatc gatcaagaat atatagaagc
     8461 agaattcatt caattgtatt caccatcatt tattgaaaag ctatatcaat ttttggacga
     8521 gcaacatttt tctttcaaat cttttatggc tgcttacaaa ttttattctc aatatgcgtt
     8581 gaaaaccaac gacggttccg cttatctgga aacttatgaa gatcgagtag ccttcaatgc
     8641 gctttacttt gcgaacgggg atgaagaatt agcacttgct cttgcagacg aaatgatcca
     8701 tcaacgatat cagccagcta caccttcttt cttgaatgct ggacgtaaac gtcggggcga
     8761 attggtgtct tgtttcttga ctcaagtcac tgacgatatg aactctatcg ggcgctcaat
     8821 caactctgct ttgcaattgt cccgtattgg tggcggcgta gggattactt tatcaaatct
     8881 tcgtgaagcc ggcgctccta tcaaaggata tgagggtgct gcaagtggtg tcgttccagt
     8941 catgaaatta ttcgaagata gtttcagcta ttctaatcaa ttaggtcagc gtcaaggagc
     9001 tggcgtggtt tatttgaacg ttttccatcc agacattatt gctttccttt cagcaaaaaa
     9061 agaaaatgca gatgaaaaga ttcgcgtaaa aactctttct cttggcgtga tcgtacctga
     9121 taaattttat gaattgacac gtaaaaacga agatatgtat ttatttagtc catatagtgt
     9181 cgaaaaagaa tatggtgtcc cattctctta tgtaaatatt acagctgagt atgatcatct
     9241 agttgcaaat ccaaacattc gcaaacaaaa aatcaaagcg cgtgatcttg aaaatgaaat
     9301 ctcaaaatta caacaagaat ctggctatcc ttacgtaatc aatattgata cagcgaaccg
     9361 cgagaatcca atcgatggca agatcatcat gagtaattta tgttcagaga ttttacaagt
     9421 tcaaacgcct tctgtgatta acggcaaaca ggaatatgaa gtattaggaa cggatatctc
     9481 atgtaactta ggctcgacaa atatcgtgaa tttgatgcaa agccctaact ttggtcaatc
     9541 tgtgcgtacg atgactcgtg cattgacatt tattacagat gcatctgata tcgatgtcgt
     9601 accaccgatc caaaacggaa ataagctgaa tcatacgatt ggattaggtg ccatgggctt
     9661 acacacttat cttgcaaaag aacaaatgga atatggctca gaggattcat tagactttac
     9721 aaatatttac ttcatgctgt tgaattattg gacacttgtt gaaagcaaca atatcgctcg
     9781 tgaaagaaaa caagttttcc ataattttga aaaatcagct tatgctgacg gcacttattt
     9841 cgaaaaatac gtaactggag aattccagcc aaaatcagaa aaagtcaaag cattgtttga
     9901 aggcattttt atcccaactg ctgaagattg gaatgctttg aaacaagcag taatgaaaga
     9961 tggactgtat catcaaaatc gcttggcagt agcaccaaat ggctcaattt catatatcaa
    10021 cgatacgagt gcaagtattc atcctatcac tcgcatgatc gaagaacgcc aagagaagaa
    10081 aatcggtaaa atttattacc cagcgcctta tctatccaat gagacgattc cgtattacac
    10141 ttctgcttat gatatggaca tgcgtaaagt aatcgatgtt tatgcaatgg cacaacaaca
    10201 cgtcgatcaa ggcatgagtt tgactttatt catgcgttct gaaatcccag aaggcattta
    10261 tgaatggaag acaacaacga agcaaacgac acgtgatttg aatattttaa gacattacgc
    10321 tttccacaaa ggaatcaaat ccatttatta cgtacgtacc tttactgatg atgcagaaga
    10381 aatcggaagc aatcaatgtg aaagctgcgt gatctaatat catagataga ttcgcttatt
    10441 catattttaa ttaaaggagc attctcatgt cagaaacata ttatgaagca ataaactgga
    10501 atgaaattga agatatcatt gataaatcca cttgggagaa gctgacagag caattttggc
    10561 tagatacacg tatcccactt tcaaatgacc tagatgattg gcgtacattg tctcaattgg
    10621 aaaaagatac agtaggacat gtatttggcg gtttgacact gctagatact gtccaatctg
    10681 aaagtggcat ggatcagttg cgtaaagatg tccgcactcc ccatgaagaa gccgttttga
    10741 acaatattca attcatggaa tccgttcatg ccaaaagtta ttcttctatt tttagtacac
    10801 tgaatacgaa aaaagaaatc gaagaaattt ttgactggac aaatacgaat ccttatctgc
    10861 aaaaaaaagc tgagcgtatc aatgaaatct acaaacacgg tacaccgcta gaaaagaaaa
    10921 ttgccagcgt ctttttagaa acattcttgt tctattcagg tttttatact cctctttatt
    10981 atttaggaaa caacaaattg gctaacgtag cagagatcat caaactaatc atccgagatg
    11041 aatctgtaca tggaacctac atcggttaca aattccaatt aggattcaac gagttacctg
    11101 aagcagaaca agaaacgtta aaagactgga tgtacaatct tctttatgag ctttacgaaa
    11161 acgaagaacg ttatacagaa gaattatacg atccaatcgg ctggacagaa gaagtcaaga
    11221 cattcctacg ctataatgcc aacaaagcat tgatgaacct agggatggat ccactctttc
    11281 cagatactgc caatgatgtc aacccaatcg taatgaacgg gatttcaaca ggcacaagca
    11341 accatgactt cttctcacaa gtcggaaacg gctatttgtt aggtcatgta gaagcgatga
    11401 aagacgacga ttatttgatt ggattggaat aaggatttat tttaattcaa aaatttttcg
    11461 aaaaataaaa agagtttagc gtacagatat tttaaatgta cactgaactc ttttttatgc
    11521 ttattcactt gtttcttcca gcacttttgg actgtctaac ttttccgaaa catgttccat
    11581 tcttctcaat aaatagattt tccggatatt taatattatc gtcttaatca ctgcatagac
    11641 aggtatgcaa atcagcatgc ctaatattcc tgcgacactt cctgctccaa tcaataccaa
    11701 gatgatcgtc aaaggatgga tattcattga tttcccaaat aacaacggct tgatcaaatt
    11761 tccatctaat tgttggatca ctaaaatcga caacgccata tacaaagctt gaaatggaga
    11821 gataaacaaa ccgacaatga ttgctggtac cgctccaata aaagggccga catacgggat
    11881 aatattcgtg attccgcaaa tgaccgcaag caacaagcta taaggctgat cgaatattct
    11941 cattgccaaa aagctcatta caccaataat caaagcatct aagatcgtac tgctgatata
    12001 ggcagataat gtttcgttta attcttttac cgtttggcgt aactctttac gaatcgcttt
    12061 cggaaagaag tgagacaacg catcaataaa tttgtgccca tccttgaaca taaatagcaa
    12121 aataaatgga acggtaaata acaaaataaa aaattgcatc ataaatgaaa tgatttttga
    12181 gacacttgca gataaactga ctaatatgat attgaacaaa ttactaagag ataaatttgc
    12241 tgatgctaat tgttcttcca aattgaaatt tttaaactct tcccgctgcg ccaattcatt
    12301 taaccatttg cttgtttcat ctgcatacat tggcaagctc tgggtcaatt gaattgtctg
    12361 ttccattaac tgaggcacta cattcatgac agttaaaaca actaaaatga ttacgaccaa
    12421 aaacgtcaat aaaaaaccga acactctcgg gatccgatgc ttttcgagaa aaacaacgat
    12481 tggatcgaac atataataga taaaaccagc aacaagcaat ggtaaaaaaa ctgccgaaag
    12541 cattttcaat attggactaa aaatattcgg catttgtaaa ataaagtaca cgccgatgat
    12601 cgtgagtact agctccactg tccagaaaaa taattttgat tgtttaaaac gtgattgcat
    12661 acatctcctc caaggtcctt tgatacttct attctatcat ttaacaaaga gttacgcgct
    12721 gagattttca tttgcttaaa aaaaacaaaa aaaataaaca tattttaatg agtagacgag
    12781 tacttaaaca aaaacacggt ttggagtagt gacaaaaatt atactgcagt ccctttttcc
    12841 aaataaacgg tgttcaaaag cttgcatctt cgtaataagc ccagccaaac gaaaaatata
    12901 aggaactatt tttatttggc tgggcttatt acttgatgta ggacatctat tttacaacct
    12961 cagatcattt attgcgcaat actgactaaa tgcaactttg tgatattttt gtgtacggac
    13021 aactcctcga tcacatcggc ataagtgagc ccttttggaa gatcaatcgt atagatattt
    13081 ttgtacactc ggtagtcatc aacgaatttt acgtcaaaat tcacatcttc gattacgata
    13141 tttttctctt caaaataatc ggtaagaaaa tctttggttt cttttctatg gataaattgg
    13201 atttctaact ttttagtcgt aggtacatgg atcacgcgtt tcactactgt gagtgcaata
    13261 gcaatcccga taaaactgat gatcgcaatg gaatagtaac ccatgccgat ggcgatccct
    13321 aatccagcaa ttgcccataa agaagcggca gtcgtaagtc ccgtcaccga ttgtttcgtg
    13381 acgatgatcg ttcccgcgcc taagaaaccg acaccactca ccacttgagc aatcagcctt
    13441 gcttcatctg aacgaatcgt tccagcaagc tgtggatact ctaaagcatc tcttaatgca
    13501 ccactagcga tctcaacttg gatcaatgca atgatacatg ctcccataca aactaaaata
    13561 tgcgtacgca tacctgctgg acgattttta tattgtcgtt caaaaccgat tgctccgcca
    13621 aatagcatag cgatccctaa acgtaaaata atctctggaa ttgtcaatac catatcattc
    13681 atctaaacac cctccagtcc aatatatcat gtctgaaagg agcagacaaa aaaagtgcaa
    13741 tttttacctc ttacgaaaaa aggactgtga caaaattttt gtcatagtcc tttttatgaa
    13801 taaacggtgt tcaatcagct gaccctcggt attactcaac aaacggcaaa atatggggag
    13861 ctatttttcc tttgtcttct ctaatactca ggtcaaaacg ctgatttcac aacctcttta
    13921 gcctactatt gaaaattaca acttacttgc tgcttcttca tcgatgatca caacaacatt
    13981 gtcgtgattt tgcaatacac ttgctggcaa atgattcgtt acaggtcctt caatcatccc
    14041 tttgattgca tcagctttgc tttcgccaaa tgccatcaag acgatttctt tacttttcat
    14101 aattgaaccg attcccattg aaacagcttt tgttggtaca tcttctgctt tatcgaagaa
    14161 tcgtttgttt gcattgattg ttgattctgt taagtcaact actgaagtcg ttccgtcaaa
    14221 tgaagcacca ggttcattga aaccgatatg tccattttgc ccgattccta agatttggat
    14281 atcgattgga tgtgcatcga tgatttgatc atactttttg catgctgctt ccagatcttc
    14341 tgcttttcca tctggtacaa acgtttcttt gaatggtttt ttatcaaata attggacatc
    14401 catgaaatga cggtagcttt gttcatctga accgcctagt ccaacgtact catctaagtt
    14461 tacagaagtc atttcactga aatccaaatc gcttgaagtc atttctttat ataaagtaac
    14521 tggtgtgctt cctgttgcca atcctaatac ttttgcacct ttgttcatac cttctttgat
    14581 gatttcaaat gctttttttc cgccttcttc agcgtttttt acacgaatga tttccatagt
    14641 gattgctcct ttatattttg gtatagccca attatagcat tctttttttc tttttacaat
    14701 aattagtata gaccaaattg catttttttc attttcttga caataaatag gaatccgtgt
    14761 taggtttcat aagatggttc aatcaaacaa tccatgttgc atgaaaacca agcaagtgat
    14821 tcactgtgga cgctttatgt ctgcagtttt tttatgaaaa aggagttatt atgagtatac
    14881 tgacattaaa caacattacc cagcaatttg gcgacaaaat cctctatgaa ggtgtcgatc
    14941 tgcaattaaa tgctggcgaa catcttgggc tgattggaca aaacggagcc ggtaaaagta
    15001 cattgattaa aatcatcact ggtgagatct taccagatga cggacagatc cattggcaaa
    15061 aaaatattca caaaggttac ctcgatcaat atgtggaagt cgatgaaaca ttaacaatcg
    15121 aagaatttct aaaaactgcg tacgcaaaag aatacgaaaa ggaagaaaaa attgcttttc
    15181 tttaccaaac atatagcgag accttcgatg attccttgct ggaaaaagct gggaaactcc
    15241 aaacggaatt agaccaagga gtgttttatc aaatcgacac cttagttgct gagatgagca
    15301 gcgggttggg gatcgatgtc ttaggtttag attcactgtt gaaaaatcta agtggtggac
    15361 aacgttcaaa ggtgatttta gcaaaactac ttttagaaaa cccagatgtt ctgatcttag
    15421 atgaaccaac caatcactta gatgatcagc atatccaatg gctgacgcag tttcttcagg
    15481 attttgacgg tgcgtatatc gtgatttccc atgaccaaga atttcttgat cagatcacca
    15541 cccatatcgc agacatcgag tttggaaaga ttactaagta tactggccat ctaaaacaag
    15601 cattgaaaca aaaggaacaa aatcgcgaaa gctatttgcg ccaatatcat gctcaacaaa
    15661 aacaaatcga aaagaccgaa gcttatattc gaaaatacaa agccggttcc cgatcaacga
    15721 tggcaaaaag ccgacaaaaa caattagata aaatcgaacg gctgacgcct ccagcttcag
    15781 caagtaaagc tgtctttgat tttccattct cgcctattgt cacgacatta gcagtggaaa
    15841 caactgattt agtcatcgga tatgatcgac cgctacttgc acccatcaat ttgaccatcc
    15901 gctttggtga aaatattgcc attcgaggct tcaacgggat tggaaaatct actttattaa
    15961 aaacactgat cggtgagatt gaaaaaataa gtggcgattt ccacttccca gataacacaa
    16021 aaatcaatta cttttcacaa gatctgtact gggaaaatcc attagaaaca ccactacaat
    16081 atttaagcag tcaatttcca aaagcgacta tcaaagaatt acgtcgccaa ctagctaaag
    16141 cagggttagt taatcaatta gcaagtgaac ctctcactac attaagtggt ggggaacaaa
    16201 ctaaggtgaa acttgcacag ttgacaatga accaaggtaa tttactgatt ctcgatgaac
    16261 cgacgaacca tatcgatcaa gaaacgaaag aaagcttaca agacgggata cagaaatttc
    16321 caggaacagt aatcatcgtc tctcatgaac aagaatttta tcaagatctt gtgcaacgag
    16381 tcattgagat tgaaccaaaa taaacgtttg attgaatagt tgaaaaaaag gagctgtgac
    16441 aaaagttttg tcacaactcc ttcttctgaa taaatggtgg ccaattagct gaccttcggt
    16501 attagctcaa aaaacggcaa aatatgagga gctattttta tttggctatt cttattactt
    16561 gatgcagaac agcttttcac aacctctttt cgtctatttt ttattttcga gatttctctt
    16621 tcctttaaaa acaactgttt gaaagaagaa cgcacatcct tgaaaaacaa gcccgctaac
    16681 cgttacacct aaccagccat actgctgcca cattgtcgtc cctagcaatg aaccagccgc
    16741 acctccaata aagtacataa acataaatac agtattattt cggttgcttg ctgcttcacc
    16801 tagcccttgt actcgtgttt gattggcgac ttgaccaaat tgattcccga cgtctaataa
    16861 aataatagct aaaagcaaaa tggccaaatg gcttccgcca accaataaag caataaagct
    16921 tattgtttgc ataacaagtc ccatatacac gatttttctt tcagaataag aatctgccag
    16981 tcgtcccacg ataggtgcag caactgctcc agacaaacca aaaatcgcta atatccctgc
    17041 ttcgaatgtt ccccaattgt aggcgggact gctgatataa aaaatcaacg tagaccaaaa
    17101 gatcgaaaat gttccaaaca tgaaaaatcc actgactgcc gcttctctta atagacgttg
    17161 agaagcaact aaatgcggca aacttttaag tgaatcaagg tatgaaacag ctgtactact
    17221 ttgctggaca gaacgcggca ttttccagta aagcagaagt gtaagaattc cgactgcaca
    17281 taaagcaaat aaatagatca tccgccaaga aaaaagactg gcaacaatac cagataccgt
    17341 ccgagaaagc aaaatcccca cgagtaaacc gctaagcaat gtacccatga ccttccctct
    17401 tgcttttggt cccgctagta ccgcaccgta aggaatgatg atttgcggca cgatcgaaag
    17461 taatcctatt aaaaaagaag cgcatgcaaa taacgtaaac gaaggcgcaa aaaaagcggc
    17521 caacagagag acggcagata acgctgccat tcgaatgatc aattttggac gatccaccac
    17581 gtcccctaaa ggaactaaaa acagtaaccc taacgcatag cctaattggg tcaacatcgt
    17641 caatatcccg actgcacttg tgctaacaga taaactagct gctacttttg ttccaatcgg
    17701 ctggatgtaa tacatattgg caacaacaac gccacaagtg attgataata gtaaaataag
    17761 cgaagttgtt aaaggttttt tttcattact cttcattgat ttcccccatt tcctatctta
    17821 gtttacaact tatggaaaag attggaaata gaaaaataca actattcgcc acacttatta
    17881 tttgtgcttt tgttttcctt gttttttctt gcgatcttta taaatgaaga caactaaccc
    17941 tactagcaca gtaacacttg caaccaacgt gactgcagtt ttgttatatc cgtgaaacag
    18001 agcttctcca ccacacgcat acaacacaga cgaagggatg actccaatca cgatcgacaa
    18061 tatgatttgc tgtagaggca atttcatcgc atctgcagca aaattgacca tagaagatgg
    18121 aataaccggt atcatatagc ctaacattac acctattttt ggatgtttca tttgtcggat
    18181 tgcttgcacc caatgattcg tttcggtttt cttatctaaa aattttaagc gatgcataag
    18241 gaaaattgca gtgatgttac caagagcatt acccaaaatg ttgatgatcg cacctgtcag
    18301 tggtccaaag ctcaaaccag caatgactcc aactactgaa gtaggaatac ctggaacagc
    18361 acacatcacc gctgtcaata cgatcaagag tatcgatgca aaagtcccat gcgaacgaat
    18421 atgtgcgagt agctctgcct tactcgctct cggatttaaa aataattgga tgtctgtctg
    18481 atattctaaa tacaaccgat agcccactac cagtatcaga atgattccca acgagatcaa
    18541 tatcaatcgg attattttct ttttcatatt tcctcctgaa aaaaatctat attcattaaa
    18601 taatagttta acataaaaac aaaaaagcac cgaaatccaa cataggatct cagtgctttt
    18661 aaatagtaaa aattatttaa cagttacaga tgcgccaact tcttcaagtt tagctttcaa
    18721 ttcttcagct tcttctttag atacgccttc tttaactggt gcaggagcgc catcaactac
    18781 agctttagct tctttcaagc ctaaaccagt tgcttcacga actgctttga taactttaac
    18841 tttttggtct ccagctgcag ttaattctac agtgaattct gtttgttctt cagcagcagc
    18901 agcgccacca gcagcagcta cagctacagg agcagcagca gatacgccaa attcttcttc
    18961 aatagcttta actaggtcgt taagttctag gatagttgct tcttttaact cagcaacgat
    19021 gttttcaatg ttcaatgcca ttataatttc ctccgtttaa ttgttctttt ttatttaatg
    19081 ctttatgcgg caattaagct gcatctccat cttctttgtc tgccactgct ttgacagcgt
    19141 aagccacgtt gcggactggc gcttgtagta cagatagcaa catagataga agtccttcgc
    19201 gatttggcaa ttttgccaat gctgtgattt cttccaatga agagacttta ccttcgatga
    19261 taccgccttt gatttctaat gcttttgctt ctttagcaaa ttcgtccatg attttcgctg
    19321 gtgcgactac atcttcgtta ctgaatgcaa cagctgtagg tccagtgaat acttcgtcca
    19381 tgccttcaag gccagctttt ttagctgcac gtgaaagaat agaatttttg ataactttca
    19441 tttctacgtt agcatcacga agttgtttac gtaaacgtgt tacttcatca actgttaatc
    19501 cgcggtagtc aacgactact actgatgcag ctgctgtaaa tttttcgctt acttcatcaa
    19561 cgattgctgc tttttttgcg ataattgctt cactcatttt tgagtttcac ctcctgaatt
    19621 ttgatggaag agttttttgc actgaaaagg caagtcactt ttatagttac tttgctcaac
    19681 cagtattatt catcacctat tcacttcgtt catagtgatt ctactcgctt tgctcgaaag
    19741 gaaactcctt tcgctttgct caaccagtat tgttcatcat ctcttcactt cgttcatagt
    19801 gattcacaat aaaaaactct atgccaccgc agacatagag ggactattga atatattcaa
    19861 taaacgtcct cggtaggaaa ttaaggcttg atgccaccta ctgtcttcgg tacaagttaa
    19921 ttattaacct caactagctt agcatgctaa actagttgag tcaagaatta tttttcagtt
    19981 ggatcaatca acattctaat atttaattag aaagaagctt ggtcaacatg gataccaggt
    20041 ccaaatgttg ttgtgatcgt tacatttttc atgtattgac ctttagctgc tgatggttta
    20101 gcttttagca atacatcatg gattgctgca aagttttcaa ctaatttttc atcgtcaaat
    20161 gaaacttttc cgattggtac atggatattt ccagctttgt ccacacggta agttacttta
    20221 ccagctttca cttcgtttac tgcttttgtt acgtccattg taactgtacc agttttaggg
    20281 tttggcatta agccttttgg tcctaatact cgtcctagac gacctacagt agccatcata
    20341 tcaggtgttg caacaactac atcaaagtca aaccatccgt tttggatttt ttgaaccatg
    20401 tcgtcgtcac ctacgaaatc tgcaccagct gcttcagctt cttttgcttt gtctccttta
    20461 gcaaaaacta aaacagtttg tgttttacca gttccgtttg gtaatacaac agcaccacgg
    20521 atttgttggt ctgctttttt agggtctacg ttcaaacggt atgcaacttc aactgttgca
    20581 tcaaattttg caatgtttgt ttcttttgct aaagctactg cttcggcaac aggataaact
    20641 ttacttgaat caactttttt taatgcatct tgcatttttt tgctcttttt agccattttg
    20701 gttcctcctt gattgtggtt ataacggttg tacctcccac gtgttctata tctttcaata
    20761 tagagccaac tgagaagctg ctgtcttagg gtgtatagat tattctacag tgatccccat
    20821 gcttcgtgca gttccttcaa ccatgcgcat tgctgcttca acgttagctg cgtttaggtc
    20881 ttccattttt aattcagcga tttctcttac ttgatcgcta gaaactttcg caactttatt
    20941 tttgtttggt tcgcctgaac ctttttcgat tttagctgct tttttcaata gaacagcagc
    21001 tggcggtgtt tttgtaacga atgtgaatga acggtcttcg tatacagaaa taacaaccgg
    21061 aatgatcaaa cctgcttgat cagctgtacg agcgttgaat tcttttgtga atcccatgat
    21121 attgataccc gcttgaccta gtgcaggacc tactggtgga gctggagttg ctttacctgc
    21181 aggaatttgc aatttaacga ttttttctac tttctttgcc acgagacata cctccttaag
    21241 tccgtgatgt ggttaattgg agatgcatat ttctcctccc acgttttatc tgatctgaaa
    21301 aaatccagac aaataatgtg caaaacaatg cacactggta cagtatagca aatttatttg
    21361 ttttttcaag atttttttct gctattttta cattttgctt gaataaacgc taaatttatt
    21421 ctaaaaaaac aacaattttt cgttctcatt tcttctttta tgttacactt gcgctagcat
    21481 ggttcaacag atgaaagcaa acaataacac ggttttttag acccttgtac aaagattcct
    21541 acataacaaa atgaggtgga gaaaatgaaa gatttaaggt atagaagtgt ctttgatatt
    21601 attggacctg tgatgattgg tccaagtagc tctcacactg ccggagctgc tagaatcggg
    21661 aaaatcatac gtagtatatt tggcgaacag cctgattctg tagatattta tctatatgaa
    21721 tcatttgcaa aaacctatcg ggggcacgga acagatattg cattagttgg cggcctgtta
    21781 gggatggaac ccgatgatga acgtttagct gattctttga aattggcata tgaagccggt
    21841 atggaagtct tgtttgttcc taagagtgaa aaagccgaac atccaaactc tgtaaagatg
    21901 cttctaaaaa aaggaacacg aaagctttca gtcactggca tttcgatcgg cggtggcaat
    21961 atccagattt ctgaattgaa tggattcaaa atttctttaa gcatgggtac tcctaccttg
    22021 atcgttgtcc accaagatgt tccaggtatg attgctaaag tgactagtat actttctgag
    22081 accaatacga atatcggcac tatgacagtg acacgggaat caaaaggtga aaatgccatt
    22141 atgataattg aagtagatga tcctcaagta gaagaaacgg taaaaagact caaacagctg
    22201 ccaaatatcg acagcgtaaa ttatttcgat taaggagcta aaaaaatgtt tttatcgatt
    22261 gaagaattag tagatcaagc aaaagcttat ccaagtgtag cagaattgat gattgcagtt
    22321 gaaatggaaa tgagtggccg ttcccgtagt cacatcatcg agatcatgga aaaaaacctc
    22381 tcagtaatgg aacaatcaat caacgaggga aaagaaggtg ttacctccgt cacaggaatt
    22441 accggtggtg atgcaaaaaa attagatgat tatttaaatg aggcagactt tttaagtgga
    22501 gaaacaatct taaaagctgt acgcaatgct gtagctgtta atgaagtcaa tgcaaaaatg
    22561 gggttgattt gtgctacacc gactgccgga agcgcgggtg ttgttcctgg tgtgctgatg
    22621 gctgtccgag atcggcttga tttgacacat gaacagcaat tggaattctt atttaccgct
    22681 ggcgctttcg ggttagtcat cgcaaacaac gcttctatca gtggtgcaga agggggatgt
    22741 caagcagaag ttggttctgc aagtgcaatg gcagcagcag ctttggtttg tgcaaaaaat
    22801 gggactcctc atcaagcagc acaagcagta gccattacat tgaaaaatat gatgggactg
    22861 atttgcgatc ccgttgcagg cttagttgaa gtcccgtgtg tcaaacgtaa tgctttaggc
    22921 tcttctcaag cttttatctc tgctgatatg gcattagcgg gtatcgaaag tgtcattccg
    22981 ccggatgaag tcgttgctgc aatgtaccaa gtcggacgtc aaatgccgtc gattttcaaa
    23041 gaaacagcag aaggtggctt agctgttacg ccaactgcta aacgacttgc ggaagagatt
    23101 ttaaataaga gggcgtgata aagctcctta atttcgagta ttaagagatt agttatcttc
    23161 ataacaaaaa acgaagagag gactaattgc ccttctcttc gttttttatt aatccatcaa
    23221 tttcgtattt tttacttcat cgatcgtttt tgttcctgca agttgcatcg tgatcgacaa
    23281 ttctttgttc aggtgttcaa aaacagatgt tactccttct gccccaccta gattcaaccc
    23341 ataaatcact ggtcggccaa tcgcaactaa gtcagcacca cttgccagag ctttgaagac
    23401 atgctctcct cgacggacac cagagtcgaa aatcactggt acacgtttgt tgactacttc
    23461 ggcaattttt ggcaatactt caaaggacgc aggacctcca tctagttgac gtccaccatg
    23521 attggatacc cagataccat ctgcccctgc agagatagcg attactgcat cttctggcga
    23581 ttgtattcct tttacgataa ctggaagatg ggtgatttct ttgattgttt tgatgtcatc
    23641 tggtgtcaag ccttgttttg cagctgcata aatttctgcg attccttttc cttcgccatt
    23701 accacttgca ctttgttcgc tatacgcagc taagttaggc attggtaatg gaaattggaa
    23761 ttgattgatg acgtcttctt cacggtaacc accgagcgtt gaatctgccg ttaaaatgat
    23821 agctttagcc cctgcttcta ctgctttgtt caaaatgaac tcattgaatc tgtcgtcttt
    23881 actcatatat aattggaaaa actgcggtgc gcctggcgct gcatcggctg cgtctttgat
    23941 tgtcgtattt gcataagtgc tgatggaaaa gattgatcca gcagcagcta ctcctttagc
    24001 tgtatcagct tccccttttt catgtgccaa tccttgggcc gctgatggcg cttggataat
    24061 cggtgtatct aattcaattc cgaaaacaga tgtatgcaaa tcggctgagt cgattccgcg
    24121 taatatccgt ggcatgattt tctttgtatt aaaagaagtc gtattttctt tcatcgtcca
    24181 ctcatcttca gaaccaccgc gaatatagcc aaaagctcct gcttccatgc gctcttttac
    24241 acgaccttct agttaggcaa tgtttacaat atcgattgga tgttcttcat tacttgtatg
    24301 ataagtcata gtagatcctt ctttcatctt cttgattaca tataaatcat actaaactta
    24361 caaaaagtaa gcaaacattt actctcagat acttttatcg ctattctttc caaataaatc
    24421 gacttacaag ctgatctact tcttggtttt catgaagcat actatgagag gcattttcac
    24481 ctgtcactac tttttcctct atttcatttc cgcgttgctt caacaacgca tagaccgcta
    24541 atgcactgtt taacggtact gttccatcac tcaagtctgt ttcatctagt tgaccagcaa
    24601 tcaagaaaaa tcgagtggtt ataggaatat taccgatcat ttgctggtaa tcttgatagc
    24661 ggttagaaac gacagcaggc ccctttgcta actcatctgt cagagactgc gcactatcat
    24721 cgacaaaatc attaaaaggc gcgccaattg ctgttaatgt ctgaacttgt ggcaatgacg
    24781 tatcctgtcc atacgttcct aaatagcgta gtgcactaac tcctcccatg gaatgcccca
    24841 ctatgttgac tttatctatg ccgtagtttt ctttcagata caaaagacaa gttttgatcc
    24901 attctgcttg attccactcg ttatttttat tatcttcaaa caatacttgg accatcggat
    24961 tgtctgcttt tccagacaaa tctcctgttg cttgtatttg tccatctgtt cctactgtta
    25021 aaaccaattc ttttactgct tgctgatttt tttccaaacg ccgcaacatc cctttaaagg
    25081 aattttctgt cccgctgtag ccgtggaaaa aaagtgttgg aatagtggtt ttttctatca
    25141 ctttactgga ttctgccgtt ttttctttat cagaaacttc agtagtattt atcttcgtcg
    25201 tctggcaacc acttaacgca aacaaactga taaggactaa agctgctgca gttgcacgtt
    25261 tgcataaatt tttgattggt tctgtcatac acctcttcct ttccatatag aaaaaagaaa
    25321 gacttgaaag gccaatcata tgatcgacat ttcaagtctc tttattaatc gattttatct
    25381 acttgatcga aatcaagttc tgtactggtt tcacgtccaa acatatcgat gttgactttc
    25441 aatttttctt tttctccgtc aacttctgtt acttgtcctt ctaatccagc aaatgcgcct
    25501 tcgatgattt tcacgacttc gccaacttct acttctagat cattttgtcg caggctcata
    25561 ccgatagaac gcaagatatg attgacttct tctggaagta atggtgctgg tttgcttccg
    25621 gctccgtgtg aaccaacgaa tcctgtcaca ccaggtgtgt tgcggacaac ataccatgaa
    25681 gcgtctgtca tgatcatttc aacaagtacg tatcctggga atgttttatg aacgatttct
    25741 ttttctttcc catttttgac ttcagtttct ttttcttcag gaacaacgac acggaaaata
    25801 aagtcggcca ttttcatact ttgcgcacga gattcgatat ttgcttttac tttgttttca
    25861 tatcctgaat atgtatgcaa gacataccag tttttttcaa aagattccat tgaaggctcc
    25921 tcatttcatt gtaaaataaa aaaacctcag tacacccgaa gtttttcttt cgcttcatta
    25981 tagcactaaa agtacgttgt ggctagcgag ttattttaaa atccagccaa ataagctttg
    26041 gatggctgta tccattacgt aaaacatcaa tccgaaaata atagaagttt cgatgactac
    26101 taacgtatct ttacggagtt gttttctgct tggccaagtt acttgtttga tttcatcttt
    26161 tacgcttttt agaaatttca tgagacaacc tcccttgcta ctgtttattt cgtttcttta
    26221 tgtaatgtat attgattaca atatttacaa aacttattga tttccaaacg ttcgccgctt
    26281 tttccttcac tgactgattt tgtgtagttg cgagacccac aaacggaaca agcgagtgct
    26341 gcttttttct ttgccattta cttctgcccc cttgctgaga atcagactta tacttcataa
    26401 cagtatcacg taacttttta cttgtcaatt tgtgaaataa actatctatt gttttggata
    26461 aaattatgat atgattaatt tgaaattaag ctaaaatgag acaattggag gtatagcgat
    26521 gctattaaaa acacttgtat ataagaaaaa agatctgaca accgttacag agactagtac
    26581 attagaagaa gcattaaaaa ttttagaaac ttccggatac cgttgtgtgc cgatcttaga
    26641 ttcttcaggt aatattttcc gtggaaacat ttacaaaatg catatctatc gtcataaagc
    26701 aaatggcggt gatatgacgc ttcctgtcac tcatcttttg aaaaacgcta caaagttcat
    26761 ttacctggat acttctttct tcaaagtatt ctttacgatc aaagagcttc cttatattgc
    26821 tgtccttgac agtgaaaatc atttttatgg tattttgaca catagtacgt tgcttaatat
    26881 gctctctcaa tcttggagcg tagaacaagg aagctatgta ctgacaattg cttccacagg
    26941 aaagcaagga gatttggctg ctattgcaaa aatcatttca aaacacagca gcattgccag
    27001 ctgtattact cttgatatcg aaaaagacga atacattcga cgtacattga tcacgctacc
    27061 ggctaacact gaagcgaaag tatgtgaaac gatcgtcaac cacttagaga aaaagaatta
    27121 taaagtggta gaaattgaaa atttgcaaaa ccaagaagca taatcaaaaa gcgaaaaaag
    27181 aaaacagcta atctgctttc ttttttcgct ttttataatt agacacgctt ttcaaaatca
    27241 agcgcgcgat ctaaataata catcaatggt gctgaaacag ccatgacgat aaactcactt
    27301 aatgcaagcg tgccataggt tgtccaaaat gcttggccac ctgttggaac taacatataa
    27361 gcaatcagtc ccatgctgat ggtaaagaag atcgtgttca atactaaacg catctttaca
    27421 tttttcactt tgttttgcaa taaagctgtc aggcctaaag aaagcaatgt ttggccgcca
    27481 ccaaaaacca catccatgat accaaaacca aagaaggcat tatagacgat gaccccgcct
    27541 aaaactcccc aaagcaattt acggttaaag acaactaaat gattcaaact ttctgaaata
    27601 cgaaactgga tcggccctga ggatacaggc gcaactaaaa tagttaacgc tagataaaga
    27661 gccatgatga tgccatttac aacgattacg gataaccgtt gtctgctttt cgttttttgc
    27721 attttctctc ctcttttctc ctagtttttt taacgcggga tggttgatga accgcggtta
    27781 tgtgaaacaa acaataatta taacagactt ttcatttatt tcaatccgtc ttatctgctt
    27841 ttaaaaaagc aaaagcttaa acaatgtaaa cgtttgctaa aaatcgtggg atatggtata
    27901 aagtaagtgt aaggggtcat aagaattccc agtaaagcag acgaagtagg atctgcaaga
    27961 acagaattgt tgtgaaactt aaataaataa aaaaggatgt gggaatttga gcaacctctt
    28021 actagagaac ggtctagcaa gcagctgaag atctaagtgg acatgccgaa ccttggaact
    28081 tctccttacg taagtaatgg atcatgcgct tgctaggctt ttttgtgttt ttcggtcttc
    28141 ggaccttttt cattttccta gacttctgct ataataacca agaattaatt ttgtaacaga
    28201 tttttttgaa aaaacaagtt tctttcattc aaaaaataga aaaagcttgc taattattgg
    28261 ttaaccagca aacaggagta ttaaaatatc ttgttttatt gaatagcagg aggaaatcat
    28321 gaaattccat tatcatatat tgatcaatcc atcggcaggc agcggaaatg gctataaagt
    28381 agcagaaaga attttaccgg tattaaaaaa taagcatatt gactatacac ttcattatag
    28441 tgaatacaaa ggacacgatg ctgagattgc tgaaactttg gcaaaagaaa cattgattcc
    28501 ttggatagaa gaagaacaga aaacaatcga tactttcccc cttttgatca tcgtaggagg
    28561 tgacggcacc ttacatcagg tacttgatac tttctatcag atggaagtag aattcccagt
    28621 tgcttatatt ccagcaggtt ctggagatga ttttgctcga ggggcaggcc ttccaaaaaa
    28681 tcccaaaaaa ggacttcagc tgattttagc agctcaatca ccagaaaaag tccacataat
    28741 ggcttatgaa gaaaaaataa gtgaaaaaaa aggaatagct gttaataatt tcggtatagg
    28801 attagatgca gctatcgtcc atgcgacaaa tcattcttcc acaaaaaagc gattgaacaa
    28861 atacaactta ggatcatttt cgtatttatt ctctattctc cgtgctttat tcactcaaaa
    28921 aggatttcca attctggtag atgcaggagg aaaacaatta agtttttcaa acgcattttt
    28981 atgtacagca acgaagcatc ctttttttgg cggaggaatc gcgattgttc caactgctga
    29041 tgcgtctaaa ccagtcattg attttgtcgt ggtcgaacga atcaatttct ttaaaatctt
    29101 gtggctcatt cttttactta tacagaaaaa acaattgaaa tcgaaatatt tccatcacta
    29161 cacaacaaac agattacgta tcgtttcaac aacaccgcaa catggtcaag aagatggaga
    29221 agaaatggaa aaacgacctt ttgatatcac aataaccaca actaaacagt tgttttggtg
    29281 ttaataaaag aggttttgtc agaagctaca tctggaacac cgtttattcg gaaaaaagga
    29341 ctgtgacacg acttttgtca cagtcctttt tttactatgc agttaattct ttgcccaatt
    29401 gggttgccat cgatagtttc tttctttacc caaaataaat tggatcattg aaaacaccgc
    29461 agcaaaagca actaagacac aaccattgaa ccaaaaagat tctgcaaatg tttgattgta
    29521 actgagtgtt tcactgatca agttattatt ttgaacaaac caatatgttg gtgtgaatgc
    29581 cgctattttg ctaaccattt ctggcaagta ttctgctggt acaaacaccc caccgataaa
    29641 gcaactgcct gtgataaaaa tatctttgat cccaccgctg gtttctgagt tttttaccaa
    29701 tgaagtgata caagccgaaa agctaatgat cggcaataaa aacaagaaag tatttaaaat
    29761 atgataccat accgttatat cccacgaggt gtggatcact aacagaacca ttagaagtaa
    29821 gaagccactg aacaacagca gcgagtaact aatcaaacta aaggttactt tgcgattcaa
    29881 gtttcgtttg gtaatgggcg cactctggtt tcgtttactg atctctttgc gattaaatgc
    29941 tagattgatg acgctgaaac cgctaaaaat ggatagaaac aacccataag acaagaaatt
    30001 aaaggttgtg cccgtcaaca gctttttcat ccgctgacta tatcccgcac ttagctcaac
    30061 ttctccagta accgagagtg ccatttgtgt ctgttccata gctgcatcga ttgttttatc
    30121 ttcttcttga aacagaatca gtgtttgcaa aaaggtgttc acttgctgtg taacaactgt
    30181 tttagaaaag gcatctgggc gactctgtat cttgatcgtc ggcatctttc ctacttcgac
    30241 ttgttcggta aagtctgcag gcagatgtaa gataatgttt acacgattaa aataaagagc
    30301 atcatcgatt tctttttgtg aggttccatc caatgtaacg actgtttcct tgtcattcaa
    30361 atatttacga aggccatcga ctacaggcga ctcttcttca ctaatcaagg cgattttcgc
    30421 atcttgcaga gaggtatttt caacatcaat attttgcgca aaaaagaatg tggcaacaac
    30481 agtgataatt acacctatca gtaaattcga tttatttgct ttaagcaatg tccaaatcgt
    30541 tttataaagc gtcataacgc acccctcttt ccatccaagc atttcctaac gcaaaaagga
    30601 aaataaatcc gatcaaccat agaagattgg tataaaaact agccaaatca gtgtagaaat
    30661 aaagtttata caaactttcg ctgatcaagt tgacgagatt taattgacca acaatcggaa
    30721 tatgaagatc aatccaatac ttcacttgtt ccgaccccat cataccagca aaaaagctca
    30781 tcgcaatcgt cactgaaatc aagatgctat ccttttgcac cagattcatt tttggtatca
    30841 aattaccaac caatgtacct aacaaaatcg cacacaacga ccccagagcg gtaacaatca
    30901 tcaaccattg ccaacgttca ccaaagtcta cttgatagat aaaacgaaag acagctaaca
    30961 taatataaat ctctgtcaaa acaatcgtaa agcttgctgc caaattagcg attgaaacta
    31021 acgattgctt tctaggaatc aaggacaagc gaataccatt tgacgattga tttgcttgct
    31081 gatcctgtac attgcggacc ccccacatcg tcccgtagag aatcgccatt ccaatcagag
    31141 tgaagaaata aaaacttttg aaactcatat tacgacctgc atgaacttct tgaaaagata
    31201 cttcttgttc aaaaacagtc atgatgggcg cgagatcaac gttctccact tctgccactt
    31261 tactcgaaat tataccaata cgctgtaaaa attgatccat gacgtttttc atcaacgtct
    31321 gactcattcc tgcttgaccc acatggagcg aaacagatga accatcaatg agataaaaac
    31381 cagccaaatc atctgcattt agcttttcat tcgcttctac ttccgatagt tcctgtactt
    31441 caaataatgg tttatcattg acttcgactt gttctaagac tgtggtaaaa ttttccgaaa
    31501 gcgctgtttg atccgctttg attacaattc cagtattaat cgtttcgaga ttgccacctt
    31561 gatcaatctc gccaaaggcc gccataaaca tcaatcctaa aagaattgga aaggccaatg
    31621 tccaaaacaa catggattta tttcgcagca gtatttttgt gcgatattta taaaaatgcc
    31681 aaaacattat ttcccctcct ctagtctctc aaatccttgc cagtaatctc taaaaagaca
    31741 tcattcaagg acgcttcttt ggttgctatt tttccgtaac tgatcttttc ttcttgtaag
    31801 attgtaagaa tcggcaacaa tactgtattt agttcagtag ttgttacttg gagctgacct
    31861 tttttataag cgattccatc agaagctatt gttcgctgac aacacgtttt ctgggagtaa
    31921 tgggatgtcg atcgtcaaac gatccgccgt ccgtaccata tcttttagag attctttggt
    31981 tcctttagca atcacctgtc cttgatccat gatcacgatc tggtcgcaaa gaatttctac
    32041 ttcttccatg taatgtgttg tatagacaat ggttgctcct tgtcgattca gctccttgat
    32101 actttctaaa attttattcc gactttgggg gtctacagct actgtcggct catctaaaaa
    32161 aattaaccgc ggcttgtggg caatcccaca tgcgatgttc aaccgacgtt tcaagccacc
    32221 actaagttct cctggacgaa acgaaacata tttttctaat cccactaatt gaatcacctc
    32281 ttcaatcaag gctttccttt tctctttttc ggaaacatat aaaccgcaaa aataatcgat
    32341 attttcttgc acacttagtt cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat
    32401 tgccttgaca gcataatttt caggagtcat tgttgtcccg aacaatttaa tggttccttt
    32461 gtcataattt aacaaagaaa gcatacagtt gatggcagtc gatttcccac tgccgtttgg
    32521 tcctaacaga cctaagactt ctccttcttg tacttgcaga tcaaaatgat tcaatgctac
    32581 aaaatctcca tagcgtttga ctaatttatc aatttcgata atcatcatga aaaactcctt
    32641 ccaaatttct tgttgatctg actataataa agtgcaagaa aagaaactag tgacgattgg
    32701 ctaaaccaca catgacattt gtcacaatga aggagtctgc tatgcataaa tctcggctac
    32761 tcaacgaaac tagctttcta ctggttatct cattgctttt gctggtaagt caaggattcg
    32821 ttgctatgca agttatctat ttattgatcg ccgttttatt cactggtgtc agttatttac
    32881 tgcctacaaa gccaaattgg attttccaag ttattctatt tcttttatgt gcctttttac
    32941 ctgatcttgt cctgtttctg ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc
    33001 ttgattactt gtttttgtta ggttttttgt tgatttcgac ggttggtaat catcccccca
    33061 tcaaggtcgc ctttgtcgta attttgctgt ttttttcctt ttacttacga aaaaaagatc
    33121 aacgaacgac cgatcttgag aaaaagtaca ttgtctccaa agatactcat tgggaaaaac
    33181 aacaagagtt atcggaacaa aataaagcat tggccctgtc acaagaaaca caattagcct
    33241 tgcaaattgt ggaagaacgg aaccgaattg cccgagacat ccatgacaat gtcggtcatc
    33301 tgctgtcaag tgcgatttta caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac
    33361 taaaagaacc attagaaaat ttaagtgcca ccgttcacac tgggatggat cggattcgtc
    33421 aaagtgtcca tgacctccat gagacatcga tctcttttca acactcctta gagtttctga
    33481 tcgctgattt tccttcttct gttacgattg aaggaaactt gtttgaagga ttgaatgaaa
    33541 cgcagcaaaa ctgcttctta atggtcatca aagaagcctt aaccaacagt atcaaacata
    33601 gtcatgccaa aaccgttgtt ttacattttc gaacgttgcc ggccttttat cgcttgcaaa
    33661 tcacgaatga tggcaccagc ccacaaaaaa attctgctcc cggtattggt ttgaaaacga
    33721 tgcgtcaacg tgttcaagaa caaaagggac aagtccatat tttccaagaa aaccagcaat
    33781 ttcagttaac gatcatttta ccaagggagg gacaccaatg ataacagtag tcattattga
    33841 gatgatccat ttgtgacgaa atcgttacaa accattttag aaagcacgaa agagatccgt
    33901 gtgttaggca ttggtcattg tgccaaggat gcgctagtct tatacgaaac ccatcgtccc
    33961 gatgttttgc tgacggacat ccgcatgcct aaacaaacgg gcatcgatgc tgccaaagaa
    34021 attttggcta aatttcccaa agccatcctt ttgcttttga cgacattcaa agatgaagaa
    34081 tacatccgcg aagcattttc catcggtgtc aaagggtatt taatcaaaca gaacttgcaa
    34141 gcgattattc cttctgtcaa atccgcttat aatggacagg ccgtatttgg taatgaaatc
    34201 atcgagactt tcactcaaat gatgaagaat aaaccgacga ttgatagtca tgcttatgct
    34261 tcttttagtg aacgtgagct tgcaatcatc aaagaagtag ctgccgggaa aaacaataaa
    34321 gaaattgcag atgccttgta cttgagtgac ggcactgttc gcaattacat cagccaatta
    34381 ctagaaaagc tagatttacg ggatcgcacg caattggcta tctattttta tcagcatttg
    34441 caataagtct tatctaacac aaaaaaacct gaatgatgct ccttcgcatc attcaggttt
    34501 ttttcacatc ttctattcaa ttgtcccgca aaccatttcc tcacgattga aagaatcaag
    34561 gattcgcagg gctgccagga ttcgttggat tgttcggcgt gttaggcgga aaaataggcg
    34621 ccttgtctaa cgttttttct agttttgatt cttcacgatc gtctcgttct acatcaggac
    34681 tttcattcaa tttgaattct ttttccatct catctaactt tttaaagtca ttgccagatt
    34741 cttttgctaa tttcgctaat ttatcttcgg tttctttagc cataaagtaa cgcccccctt
    34801 ttgatttcag cttagcatat cacactgaaa aaacaaaaga aaacgctaac caacgatcat
    34861 ttttagctat ttctcatctt taaaatcaag cattttttgc cggtataaaa tcgtagggag
    34921 aactgttttc cataccaatc atcggatcta ctaactggtt atcgatcact tctgctgtaa
    34981 gaacgaaatc tttagaaaca gcaggttcta cctcttcttt cttttcttct ccaacaaatg
    35041 aagaaaacag atctggttcc actgtttcca ttacttggtt tttcgtttca ttcgtcccac
    35101 tgtcatcttc tacaggtagc ttttctttta caacccgtgg ctcttttgta tagttcccat
    35161 cttcaaatgg atcatttccg accaaagaat cttcataagc tgctagtttg gtccggagag
    35221 tgatattcat tttatcatta tccatgatcc ttgatttcaa cgtagtcatc cggcttgctg
    35281 attcattcgc tacacaagtg acataggctt gctcttcccc taacaaaatc aacagttcct
    35341 tactgcgagt cactgccgtg tagagcaaat tacgctgaag catccgctga tattgatgga
    35401 ccatgggcaa aataaccatc ttgaattcac tgccttgtga tttatggatt gagcaacaat
    35461 aagaaagagt gattttattc cattcgtttc ttttataagt gacctcattt gcatcaaact
    35521 ggataactaa ttcatccact ttatcttcag aatcttttgc caagatgata cccgtgattt
    35581 ctcccatatc tccattgaat acattcaatt cgggagtatt gactaattgg aggactttgt
    35641 ctcctatccg ataaacggtg tcgttccaat gtacttcctt ttttccaggt ttccctggat
    35701 tgaatatctc ctgcatcatt ttatttaacg catcgatccc tgcaggtccc cgatacattg
    35761 gtgctaatac ttggatatct tgtgcacgga atcccttatt tttcgctttt tccacaattt
    35821 tagagatcaa gggttcaatt tgataagcat cacttcggaa ataggaacga tctttttgat
    35881 tctttgtgaa atcttttggc agacgtcctt ctttgatctc atgggcaaga ggaataatac
    35941 tagaaccatc accttgccga taaatctgat tgagttccat tttgggaatt tgttcgattt
    36001 ccaaaagatc atggagtact tgaccaggac cgacagaggg aagctgatct ttgtctccta
    36061 ctaagataac ttgcatattc gtcggaatcg ctttgaataa tgtgttagcc agccatgtat
    36121 cgaccataga catctcatca acaatcaaaa gtccgccttc taattctttc gtactgactg
    36181 aaggtgtttt ttcccgtcca ttcagcccca gaagacgatg gatcgtactg gcaggcagac
    36241 ctgtcgtttc gttcattcgt ttagctgctc ggccagttgg tgctgcaagt agaataggaa
    36301 aaatttcttg ggtatatttt gaaggatcca attccagtcc gttcaactct gcaaaaagat
    36361 tgacgattcc gttgatcaca gtagttttcc ccgtacctgg tcctcctgtt aaaataaata
    36421 gaggggaacg gattgcttca gcgatagctt gttcttgcga aggtccatac tgtatgccga
    36481 atattttttc gatacgccga atattcttct gcacttcttt ttcaggataa tggatttctt
    36541 ttttacgact gagaaggcgc tggatcgaat taccgatccc ccattcagaa aagtacaaac
    36601 tgttttcgta aagattagtc tcttcttgtt gtatctttcc ttcttcaaca agttggataa
    36661 ttacagtagc aacctgatca ggctggattt caattgggcg acttgtttct aaaatacgga
    36721 tcgtttgctg aagcaatgtc tccgccggta cgtatgtatc tcctgtttga actgcatgct
    36781 gaaaaatctg atgcaagata gctgctcgaa ttcttctcgg tgaatctgct gcaataccga
    36841 tttgttcggc tagattatct gcacgtttga acccgattcc ttctatgtct tcgactagct
    36901 ggtacggatt ttcttcgatg atatccaaag actcgttctt ataggcctgg tagatactga
    36961 aagctaactg actaccgaac ccgtaacgat tcagtcccat gatcacttga tccatcccgt
    37021 ggtttagccg aatcgtctca gcaagcattt ctctttttgg cttcgtcaaa ccggagattt
    37081 gttttaatac cgaaggatca gcaatgattt tttcgatagc atcttctccc agcaattcta
    37141 caattttttc tgctgttttc ttcccgattc ctgggaattt ttcgctggaa agatacgcta
    37201 cgatcccatt tgcagttgtt ggcttttctt gctcatatct ttcgagttta agctgccttc
    37261 cgtatttagg atgatccact aattctccat aaaaacgata agtctcattt tcttgaatat
    37321 cgccgaaact tccggtgact acaatctctt tttcacggta atcagctgtg gtttcactga
    37381 tgtgtaccaa aagcactttg taaaaattac taggattttg aaaaaaaatc gcggcaactg
    37441 taccaacgaa ataaggcatt tcttctggct gcatcggaag ccgcctcctt cttctatagt
    37501 aaaggttctg gtgctgtttc agttcctaaa aaatctgttt ggttccatac tttcagttca
    37561 tatggcaata atctgcaagg ctcttcagtc tctaaaatcg tcaaactact attgaacaat
    37621 ccacccattt cacgcaactc acttagctct tttcctgtca tccattgaat ggctgcagtt
    37681 aaagaagctc catgtcctac gaataaaagt gggccatctt tatgttgggc aaccgcatct
    37741 gctacaacag tttcgatacg tgtgattgcc gattcaatcg tttctccgtg aaaaggagaa
    37801 ggatcataca gatctaaatg attacgcaaa ttcggcagat tatccggata tttctggtac
    37861 atttcatcga tcgactgacc ttctaaagta cccagcccta gttctcttag cttatctgta
    37921 taaaaaatct ctaccggacg agcaagctct ttgttgatac cttctgctgt atcacgtgct
    37981 cgctttgctg tactggaata aattgcttta aaaggaacat attttacctt tttcccaaat
    38041 gctttgattt cttcgtaact ttgtggcaaa agcggagaat cgccattgct cccttgaaaa
    38101 cgtctttcct gattccattg tgtcttccca tgtcgggtaa aatatagttc catacgcagc
    38161 aaactcctgt ctttctttta ttttactcta taataaactg tctaatccag cttgtcattt
    38221 tcatagtgat gcgtcaattc taaattaggg aatccttgct ggcgaagtgc ttcatacact
    38281 atcattgcag ctgtattcga taaattcaat gagcggacat gctcatcatt catagggatg
    38341 cgtaaacact tttcttcgtt ttcacgcata aatgtttctg gcaaaccggt tgtttctttt
    38401 ccaaataaaa agaagtgctc ttctccatca gcaaaatttt cgtcactata tactttatgg
    38461 gcaaattttg agatcaaatg tagtttacga ttacctaaaa atgttaaaaa agcttctaaa
    38521 ttactgtggt aagtaatatt tacatcattc caataatcca accctgcccg ttttagatgt
    38581 ttgtcatcag ttgaaaaacc tagtggctcg attaaatgaa gcggagtatt cgtggctgca
    38641 cacgtacggg caatatttcc agtgtttgca gggattaatg gttcaaataa aacgatatga
    38701 ttcatcagga tcattcctct catatttatg ttcgtttcta ttttctgaca atttgatttt
    38761 tgcataaaaa aaaacgtatc aactcggtgt cagtcactgc gagtcgttac gttagtgaag
    38821 ctcttctcac taacttttat cagaacagga acctgataaa aaccaatgaa aaaacaactt
    38881 gttgctaata taacaccctc aacttttttt tgcaacctct actcattaaa aactgctaga
    38941 aaaacgtttt ctcttagttc cttttaataa ttctatgtca attgtaatct tcttcaaagg
    39001 attcttatat aagtctgctt tgattcgctc ttctacctgc cattctaaag gtgacatggc
    39061 caaaagatct ttttgatttt cctctggtat ctcaaattca taggttacac gctgacgttc
    39121 agttaatttc attgcttcag caaacttggc tactacacgt tcattggaat aactctgttt
    39181 ttcaggttga ttcggataaa aagcctgacg tagttccttt aaataatttt cttgtggcac
    39241 caccttcaac acttctccat cttctgagag tacacgttgg aattcttggt aatgagaagg
    39301 cgaaaagata tttaaaaccg cgttgaatgt ttgatcagca aacggcaaat tggtcaaatc
    39361 agctacacac caaaatgctt cggcagattg ttctgtagct gcgtagactc cttcttttga
    39421 aatatcaaat ccaacagcat tttgaatctt tttcaatttt gaaagttcat tcaagaagct
    39481 accttctccg cagccaacat ctaatagcga gacttcttca ggtaaatgct gcgcaatagt
    39541 ctcaataacg ggctgataca tgccagattg gatcattcta cgccttggaa cgaacattcc
    39601 ttgatcgtat tctgttttaa ccgcatggga taaaaaataa agtgttcctt ttttggaaag
    39661 gtcaaaacgg tgattatttt tgcatatcaa gccattatca gactttttta cttcttcttg
    39721 gcaaaccgga cagcggaaca gctgatgatt ttctgccaaa aactggcgtc ctcggtcaat
    39781 tttttttaac atagttcccc ctcatttctg ttttatctta ccgttagatg ctcactttgg
    39841 caactgctaa aaatatgata caatgcctag aagaaagtga aagataaagg agaagattag
    39901 atgatcaaag aattttgcgc tgaaaactac acatctattc ctctagcaat tgctagagga
    39961 gccgatcgga tcgaattgtg tgacaactta gcggtcggtg gcacaacccc gagtactggt
    40021 gtgatcgaag aaacgttaag ttatgctggg gaaaaagata tccccgtaat gacgattatt
    40081 cgtcctagag ggggcgactt cgtctataac gatatcgaat tgaaaatcat gcatactgat
    40141 ttgattgaag caaagaaatt agggacggat ggcgtagtat ttggttgtct tactccttca
    40201 ggctggctag acgaagaggc cttagaacta ttgatcgaaa cagcagaagg gttgcagatc
    40261 accttccata tggcttttga cagtattcct gaagatcggc aattcgaagc aatcgattgg
    40321 ctggtagaac atggtgttga ccgtatcttg actcatggcg gtcctgctga caaaactatt
    40381 gtagaaaatt tcccacggct taaagaattg attgcctata cagatggccg cttaatcatt
    40441 ttgcctggtg gtggtgtcac tgcagagaat gcagcacatg tagctaaaga attgaacgta
    40501 agcgaagtac acggaaccaa aatcgtggat cttcagttat aatcttataa acaaaaaaac
    40561 atttgcaaca ggcataatgc ttattgcaaa tgtttttgtt tttttacata aaatagccca
    40621 taaataaaga agcaatattg aaataaatca gtacactcgt caaatcgctc aaagtcgtga
    40681 taaagggacc gcttgctact gctggatcaa atcctagacg atccattagc atcgggatta
    40741 aactacctgc aagattcgcc actgtgattg caaatagcat agccatccct atgacaaacc
    40801 ccaagataaa attatgtttc caaaaaccta cgattagaaa aatcgtgatc cccgtaacag
    40861 ctcctgtgac aacgccggtc aagacttcag ccaaaacaag tcgaacaaat ccatgatctt
    40921 tctcatcaga aatcgcaagt cgacgaacag ccacagctag cgactgggtt ccagcattac
    40981 cggcagttcc tgtaatcagc gaaatgaaga ctgctaaaat actcgcttca cttaccaagt
    41041 cttcatagtg actgattaaa gaagcggtcg acatacctaa aaacaacaga gtgatcagcc
    41101 aaggcaaacg tcgagacgct gcctttactg gattttcatt aatttcttcc acattgaccc
    41161 cggcaagtcc tgaataatcg ctagcagctt catcatcaat aacgtcgatg atatcatcga
    41221 ctgtgacgat tcctaacaaa tgatcatcat aatcggtaac aggaagagct aagaagtcat
    41281 aatctctaaa tgtttgtgcc acatcttctt ggtcgtcgcc tacatgaact gaaagcactc
    41341 gttcgctcat cagatcagaa atcattgcgt cgtcatcatt cacgattagg tcgcgcagag
    41401 aaataacccc tactagctga ccttcttgat taacgacata gatgtaatag attgtttcag
    41461 ctacatctgc ttcgttcttc agcacgtaca tcgcagagcg aacagtttga ttggctacga
    41521 tcgaaacaaa ttcagttgtc atgatcgcgc cagccgtttc atcctcgtaa tggagaagtt
    41581 ccttgatttc actggcatcg tctgccgaca tcaaacttaa atatttagca atttgctttt
    41641 tatccagcgt attaagcaag tcaacagcat tatctgtata catctcagaa agcatatcag
    41701 ctgcatagct aggacgcatt tcatctaggt attctttcat atgttcgttg tcttcttcaa
    41761 tcacatcgaa catatccgct aattctttag gcgacagata ggaatagatc agctgcctgt
    41821 tctcttctgt aagagattgg tagaattgcc cctgctcata gatatgcatc tctaaaaaac
    41881 tgtttctgaa tgcctgaata ttattttccc ttaaatcttg agtcaactgg gcaaatcttt
    41941 cctctaattc ttgattttca tccaaaccaa tctcacctcg ccttattctt caaaccgtct
    42001 cacaactgcc tccatgtctg cggctaaagg ctgcttgaac tccagcaact cttctgtaaa
    42061 tggatggtaa aaacgcagat cataacaatg aagggcttgt ctttttattc ccagatccat
    42121 tcgcccgcca tacatatcat caccgagcag ggcacagcca attgcggaaa aatgaactct
    42181 tatttgatgt gtccttcctg tatgcagctg gatatcaact aacgcgattt ctgttagtcg
    42241 cttctctatc cagtactcag tctgtgccga acgtccttca tcagagacca ttcgcttcaa
    42301 tagcgaagaa taatctcggg cgatcggcaa atcgatatgt ccatgtgccg ataaatctga
    42361 aagccttcca ctgactaagg cctgatattt tttcacaaac ttcttttcac gaagttgcat
    42421 atctaatttt gcgtgcgcaa atccatgctt tgcaaaaagc atcaaaccag atgtatcccg
    42481 atctagtcgc gtcaccacat ggattacttg gttttcgtaa ccttgttttt tataataagc
    42541 ctttacacga ttagccattg tcccatttgg atggtattgg gcaggaatag acgaaacgcc
    42601 tgctggttta tttactacca gaacgtgttc atcttcatac acgatatcga tcggtgtttc
    42661 atccaaaagt acggtttcat gttctccttc agcaggaatc acaatggtta ccttatcatc
    42721 cttagctaga gaaaacaaga cattttctac ttgatcatta actttgattt gaccgccttg
    42781 aaattttatt ttcgctaaca gaccctttga gatccctttt tcttttaaaa aatattttac
    42841 ttgctgagaa tgatctttct cataatccca tgtaaactgc atatcaatcc tcactaataa
    42901 aggcatcttt cacacgatgc caaaaatgca tatgtcgata agaagcaaaa tggatccgtt
    42961 cttctgctat tcgataataa attgagcgga tatttgcttt tgcgacattc aattgatcaa
    43021 ccgttacaag ataatcttct gtattttcca attttatttc tagccattca tcatgggcaa
    43081 tcacgatcgg agaacccaaa gtacgaaaca ctcgattatt cagtgaagca atctctgcca
    43141 actgaaaggc attgatacta ggatggatca cagcaccgcc gacactttta ttatatgctg
    43201 tcgaaccagt aggcgtagaa atcgtcaatc catctcctct aaaactttca aacagctcgt
    43261 ttttgatgta tacatcggct accatcgtac gattggcccg tttaatcgtt gattcattca
    43321 gtgctaaaaa atgctggtcc ggtgtttcat ccaaataact gatccgaaca tccagcaagg
    43381 gataactaac actttgttcc cggttcgttc gtaagctgtc tactagttct tccagctcgt
    43441 aatcccgcca atccgtgtaa aaccctaaat gacctgtatg cacgcccaag aaacgcacat
    43501 cattcagaca atgactgtag tgatggaacg ccgacaataa tgtgccgtct ccgccgactg
    43561 agatgaccaa ttctggctca tgatcatctc gttgaatccc tgcttgttct aaaagtgcgt
    43621 tcaaacgttc tgtcacttct atagtctttg cttctttatt atgaacgatc gctactttca
    43681 tcccctgcct cctctctttc gctttctttc ttcaaaagcg ttcgatagta gacatcatct
    43741 gattttcctc ttccatggga gaataaccgc tgtgcttctt ggatttcttc acggatactg
    43801 gacatctctt catccaactg ataggcagct tctgccgcac gctgaagtcg ttcactcatt
    43861 tcttctggaa aaacgccttg atatttataa ttcagcgaat gctcaatcgt tgcccagaaa
    43921 ttcatcgcaa gagtccgaat ctggatttcc gccaaaattt tcttctcacc gctgatcagc
    43981 tgtacaggat actcgatcac cagatgataa gaacggtagc cacttgcttt tttatttgtg
    44041 atgtaatctc gttcttggag aactttcatg tcttttcttt tacggatgat ctcaacaact
    44101 tgatggatat cttctacaaa ctgacacatg atccttaacc cggcaatatc agacatttct
    44161 tcttctaaac gtcccattgg aatatgccgt aattgtgatt ttgttacaat actatcgact
    44221 ggcttgactc tcccagtgac aaattcaatc gggacatgtc gattttgttc acggaactgt
    44281 ttgcggatcc cccgcagttt cactttcaat tctgatacag tttgttcata tggtgctaaa
    44341 aatgcttccc aatctcgttc catagctgat acctctactt cattaacatt cattgtttat
    44401 tctaccatat atgcaaagtt ttttcgtact cttgatttat gcattgacca aaaaaagaaa
    44461 attaggcaaa atagacaaaa aggagtcgat caaatgacac aaatgcttga aatcgaatat
    44521 aaatctatgc tgacaaagca agagtacgag atattcatcg ctcactatca attaacagat
    44581 aaagatttcc gtgtacagac taatatttat ttcgataccg ttgatgaaca gctgaagaaa
    44641 caaaattgtg gcctacggat ccgtttcgtt ggtcatcaag ctgaatatac attaaaaaca
    44701 cctgctgaaa aagggcgtct cgaaacaacg gataccttta taacagaaga agcagaagca
    44761 tttatcttcg aaaaacgtct gcctcgttca ggcgcagttt ttcaaaaact ttctgatttg
    44821 aatatcgatc ctgcttcatt aatccaaacc ggaaagctta caacaaaacg ggctgaattc
    44881 cctattgaag aaggactttt agctattgat gaaagctggg gagacaatct acacgatttt
    44941 gaactggaac tagaggtagg ggatgcatct gtgggaaaaa ttgcttttat aaatttttta
    45001 aaacgttttt ctttacctta tcgtcctgct aagaacaaga tccaacgtat gcttgaagct
    45061 aaaaattaga aacctttcat tttcacaagg cacttgagtt cattttttta cttttttctg
    45121 ttaagttata ggatgtgaaa gaatctagtc actagttgta tagaatctat tttgggggag
    45181 attcatatga tcgaaatcta tttatttgtt aatcctttag gggaatactg tttcgaaatg
    45241 gaacaacagt tattacaatt tatcgaagaa gagtacggga caccatcaaa agaaaagatg
    45301 caatttcgct ttcttccttt ggtcaatttg cagacaattg gggatgtcat gcaacaaaaa
    45361 ggaatttctc agaacaatct tgaggaacgt aatcacttat tctcaatgac ttattctgca
    45421 gcattggact gcaaagcggc gcagtttcaa ggaaagaaaa aaggccgccg ttttttgatc
    45481 aaattgcaag aagcagtcgg ctgtaatgac ataccatatt ctcaagaact tgctgaatcc
    45541 atatttgaag aaatcggcgg cgatttgaat atgtttaaag aagatcgtca gtctgacttc
    45601 gttaaagaag tattcttttc tgatcaaaaa attgctcggg aaatgggcat cacaaaacac
    45661 ccttctgcag tcgtttacaa ttatgcctgc gaccgtgatt ttggtgtttt agtagaagta
    45721 aaaggtacaa gtagcctttg gagaatcaca gagctgtgta aaacaacaga taactcatat
    45781 caagttttta atggtgaaca tttacataca aatgattctc agttccattc aagaactagc
    45841 catttacact tgctttctaa ttaaaaaaat acgtcgactc ccttacaaga gagccgacgt
    45901 atttttttcg tttctattcg agaaaatcaa aaatagaaag ccttataaac gatcgatcaa
    45961 ttcttctaat tcatttagac gttgttcaaa catcgacatt gcatcttcaa tatatgcagc
    46021 ctgtgtcata tcaactcctg cttttttcat tacttccaca ggataatcgc tgttacctgc
    46081 ttttaggtaa gccaagtagt tctctagtgc ctctggttcc tggtttagta ttttcttcgc
    46141 cagtgctgaa gcagcagaaa agccagtcga gtattggaaa acatagtaat tataataaaa
    46201 atgcggtatc cgtgaccatt cgaatttaat ttctgggtct tcttcgactg ctggaccata
    46261 atattttgca ttcaatttac cataactatc acttaggtat tcactagtca atggcacacc
    46321 tttttcatct tccgtatgca tgaaatgctc gaattcagcg aattgtgtct ggcggaaaac
    46381 tgttccttta aacccatcca agtaatggtt gagtacataa gcccgtacac gagggtcttt
    46441 ttctgtttcc aataaatact ccgttaggat attttcattc gttgtcgaag caatttctgc
    46501 taaaaagatg gagtagtcgc cgtacacata aggctggttc gaacgagtga aataactatg
    46561 aacactatgt cccatttcgt ggacaagcgt aaatagctga tccagcgtat catgccaatt
    46621 caataaaata tatggatttg tgtcatagct tcccgaagaa taagcatcgc ttcgtttccc
    46681 tttattttcg acaacatcga tccaacgttc agagaatgct ttttctacga tggccatgta
    46741 ttcttcaccc attggtttca gtgcttctaa agctttttct tttgcttctt cgtacgtaaa
    46801 ggtaattgga gcttccccta agaccggtgt ataaagatca tacatgtgca gtttttccac
    46861 ttctaataaa cgtttccgta attccatgta tcgatgtaac aaaggcaaat gcttgtttac
    46921 cacgtctacc aaagtatcgt atacactttc aggaatatga ttattgctca aagacgcttc
    46981 tctggcagag ctgtaattac ggactttcgc tttaaaatta tgtcctttta tatgtgtgcc
    47041 taaagtagaa gcaaatgtat ttctaaattg ttcgtaaaca ctgtacaatc ccttaaatgc
    47101 tgcttcgcgc acccttcgat ccgtgctttc tagcaactga ccatacacgc catgagataa
    47161 ttggactatt tcaccatttt ctccttcaat cgttggaaaa actagatctg cattattcaa
    47221 aacagcaaat gtatcacttg aagcatcaaa gatttcacct gctccagcaa gaagagattc
    47281 ctgctcagca gataagacat gggctcgatt atctactatt tgctggatat aatggcgata
    47341 gacttccaat tttggttctt ctttgaaata ttgccaaatc tggtcatctg acaattgcaa
    47401 tatttctggt tcaaaccagg aaacggcttc actaactttc gaaaacagac tgcttgctct
    47461 tgcataaagc gcttggtaat ctgtatttcc agtgtcttga tcgtttttaa ggtgcgcata
    47521 tacataaatg acttcagttt ggcgataaac cctcaatacg aattcaatcg catttaaaaa
    47581 ttgagaagcg ccttgatcaa gtgttccttt gtgtttttca gattgtttta actcttctga
    47641 taattccaaa tatttctcat caaactcttg gtcgcttgaa aagatcttgg tcaagtccca
    47701 agttaaattt tcaggcaatt cttctcgttt tggcaactgc tttacttcca tgaaatccac
    47761 tccttttctt cccttagctc tatctatcaa aatagatcat agtctaacat ccacatgtca
    47821 cagcatctaa cttttctact cattcatgtg tctgcttcca tcaattttag cataactaaa
    47881 tcttgtttta catagaaaac cgattcctag cctattttta tacttactat tttttcaaag
    47941 aagacaagtg tatgcattcg ttaaaaaact gctgatagac catctcttca tcaatcatag
    48001 gaaaaagcca ttccctttta tgttctttta tttggcaaag ccatgagttg aaatcaggaa
    48061 ctttctggtt ttccaaggct tcttcgtaca acattcgata aagaaaaacc tgttcttgta
    48121 aataaaagaa aaaccgactt ttttgataga tccacgatga tagatgcaaa agatgtttat
    48181 gacgcaagta gcattgttct tgtatggata ctgtattctt ttgcttgtga ataagttggc
    48241 gtgtcagcca tttcttccca tcttcactca ctttttctat ttttttcatt tggttcattt
    48301 taatttcgtt actagtgaat atttctttaa ggtccctact agaaaaagtc cagcagctac
    48361 tgcaaaagga aatatgccct gatacagtct ctgtaatatg atgatacaaa taaagctttt
    48421 gtttcttgag atcagctttc cagcaatgca ctcctctttt tctattatag tagcagcaac
    48481 ttttttctat caatgagaat tgcgattgtc caagaaaaga attaccaagt atccaccata
    48541 cttggtaact gttattcaaa taattttggg ttctttcaat aaatctctgg tgagacagtc
    48601 tagaacattg gatttctatt gctagattcg ccaacaaaat atccggcctt tgccgcaacc
    48661 tcggcaaata ggcttccaaa aatactggtt ctactgactg tcccagccaa tccatgaagc
    48721 attcctttaa ctgtaagtgc tcttttgatt ctctttcact aaagaccgaa cagttatctg
    48781 atttttgatg ggcaaaatga gcacattttt tctttccctt tttcaaaatc actctttttc
    48841 gacagccacg acaataatat ttctttcctt tttctgcatg tttagcaaaa cagcacttat
    48901 tatattcatt cattgcaagc agcatttttc tccctctgaa cttatttacg tctaagaatt
    48961 tatttacacg aaaaaagagg gcgtgaaaaa gctattctgc atcaagtaat aagaacagct
    49021 aaacaaaaat agttcccaat atttttcgtt tggttgggct tgttaccgca gatgcaagct
    49081 tttgaacaca tttatacgaa aaaaagactg cgacacaatt tttgtcacag tctcttacta
    49141 taatgctgat ttatacaaaa taaaatttcg ttcgctcgat agcattacgc gccatcagac
    49201 attgtccata ttcattcaat gtttctggtg tacgatcgct cttttcagca aactccaaaa
    49261 ttcgagcaat ctgattctct acatccgctt ggttgagatt ttccatccag aagtgaacga
    49321 ctaggtaata gcgctcttcg aataaataca aatcagtcca agcagactct agttcgacat
    49381 ctgttgcaag ttggatcact tgttcaaaat cttcaaaagc aaaagttgct tgtctttcct
    49441 gttcttctag ggcgcttcca gataattctt cctccggatc gctgccaata atctgctgac
    49501 gaagaaagtc actcacatct tctgaatcaa cagtagaatc ttctgattga gaaatttctt
    49561 catccggaag atttttactg ataaacagtt ctaatccgtc tccttttggc aaaacttgga
    49621 aagtgacagc ttcgcttcct cggaactcct cttcgatatc aacttcttcc aaaatactat
    49681 aaaagaaatt ttcgatttct ttgtgattgc caagtaaatc caaaaatgaa acgccccgtt
    49741 cctctaaatc ttcatgacca attagtacac ggatcgtatt ttcattaata tgttccattt
    49801 ccattttgct acacctcact ttgccacaat gtttaatata gattcattgt agcgaaatca
    49861 gcttaaatgt aaagaaaaaa aatctcgctg ttaagcgaat ttaataaaac cagcggattt
    49921 ttcaaccttt caagctatct tatttaacta taacattttt ggcagacgaa tactatatag
    49981 ccgacttttc agctaatttt ccctctctat ttttcgtgca atcatcatct tctactacct
    50041 ttttatacat ctaatcaaga atactttttg taaaaacaaa aaaacttgga aaaactatct
    50101 ttgtaatagt
//
LOCUS       DAFGZE010000016        47470 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.016, whole genome shotgun sequence.
ACCESSION   DAFGZE010000016 DAFGZE010000000
VERSION     DAFGZE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.016"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(56..790)
                     /locus_tag="LTU54_000938"
     CDS             complement(56..790)
                     /locus_tag="LTU54_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL8295669.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1117..1527
                     /locus_tag="LTU54_000939"
     CDS             1117..1527
                     /locus_tag="LTU54_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBL8295670.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1520..2221
                     /locus_tag="LTU54_000940"
     CDS             1520..2221
                     /locus_tag="LTU54_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBL8295671.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2221..3807
                     /locus_tag="LTU54_000941"
     CDS             2221..3807
                     /locus_tag="LTU54_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295672.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3826..4047
                     /locus_tag="LTU54_000942"
     CDS             3826..4047
                     /locus_tag="LTU54_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HBL8295673.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4044..4805
                     /locus_tag="LTU54_000943"
     CDS             4044..4805
                     /locus_tag="LTU54_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HBL8295674.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5185..5694
                     /locus_tag="LTU54_000944"
     CDS             5185..5694
                     /locus_tag="LTU54_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBL8295675.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5769..6308)
                     /locus_tag="LTU54_000945"
     CDS             complement(5769..6308)
                     /locus_tag="LTU54_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBL8295676.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="LTU54_000946"
     CDS             complement(6448..7059)
                     /gene="rpsD"
                     /locus_tag="LTU54_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBL8295677.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7321..8172
                     /locus_tag="LTU54_000947"
     CDS             7321..8172
                     /locus_tag="LTU54_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBL8295678.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8200..8835
                     /locus_tag="LTU54_000948"
     CDS             8200..8835
                     /locus_tag="LTU54_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBL8295679.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8855..9628
                     /locus_tag="LTU54_000949"
     CDS             8855..9628
                     /locus_tag="LTU54_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8295680.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10036..10833
                     /locus_tag="LTU54_000950"
     CDS             10036..10833
                     /locus_tag="LTU54_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HBL8295681.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10811..11224
                     /locus_tag="LTU54_000951"
     CDS             10811..11224
                     /locus_tag="LTU54_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HBL8295682.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11208..13853
                     /gene="mutS"
                     /locus_tag="LTU54_000952"
     CDS             11208..13853
                     /gene="mutS"
                     /locus_tag="LTU54_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBL8295683.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13871..15979
                     /gene="mutL"
                     /locus_tag="LTU54_000953"
     CDS             13871..15979
                     /gene="mutL"
                     /locus_tag="LTU54_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBL8295684.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16001..16561
                     /gene="maf"
                     /locus_tag="LTU54_000954"
     CDS             16001..16561
                     /gene="maf"
                     /locus_tag="LTU54_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBL8295685.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16646..18634)
                     /gene="treP"
                     /locus_tag="LTU54_000955"
     CDS             complement(16646..18634)
                     /gene="treP"
                     /locus_tag="LTU54_000955"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HBL8295686.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18857..19570
                     /gene="treR"
                     /locus_tag="LTU54_000956"
     CDS             18857..19570
                     /gene="treR"
                     /locus_tag="LTU54_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HBL8295687.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19647..20093
                     /gene="msrB"
                     /locus_tag="LTU54_000957"
     CDS             19647..20093
                     /gene="msrB"
                     /locus_tag="LTU54_000957"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBL8295688.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20263..20865
                     /gene="ruvA"
                     /locus_tag="LTU54_000958"
     CDS             20263..20865
                     /gene="ruvA"
                     /locus_tag="LTU54_000958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBL8295689.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20878..21879
                     /gene="ruvB"
                     /locus_tag="LTU54_000959"
     CDS             20878..21879
                     /gene="ruvB"
                     /locus_tag="LTU54_000959"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBL8295690.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21908..22486
                     /locus_tag="LTU54_000960"
     CDS             21908..22486
                     /locus_tag="LTU54_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8295691.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22538..23020
                     /locus_tag="LTU54_000961"
     CDS             22538..23020
                     /locus_tag="LTU54_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HBL8295692.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23137..23511
                     /locus_tag="LTU54_000962"
     CDS             23137..23511
                     /locus_tag="LTU54_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295693.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="LTU54_000963"
     CDS             complement(24189..25541)
                     /gene="brnQ"
                     /locus_tag="LTU54_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL8295694.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
     gene            25688..26278
                     /locus_tag="LTU54_000964"
     CDS             25688..26278
                     /locus_tag="LTU54_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL8295695.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            complement(26406..27755)
                     /locus_tag="LTU54_000965"
     CDS             complement(26406..27755)
                     /locus_tag="LTU54_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8295696.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(27923..28663)
                     /locus_tag="LTU54_000966"
     CDS             complement(27923..28663)
                     /locus_tag="LTU54_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL8295697.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            complement(28676..30268)
                     /locus_tag="LTU54_000967"
     CDS             complement(28676..30268)
                     /locus_tag="LTU54_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL8295698.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            complement(30836..31132)
                     /locus_tag="LTU54_000968"
     CDS             complement(30836..31132)
                     /locus_tag="LTU54_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295699.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            31308..32033
                     /locus_tag="LTU54_000969"
     CDS             31308..32033
                     /locus_tag="LTU54_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBL8295700.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEMAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            32026..32868
                     /locus_tag="LTU54_000970"
     CDS             32026..32868
                     /locus_tag="LTU54_000970"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HBL8295701.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELIALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            32871..33392
                     /locus_tag="LTU54_000971"
     CDS             32871..33392
                     /locus_tag="LTU54_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBL8295702.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(33645..34208)
                     /locus_tag="LTU54_000972"
     CDS             complement(33645..34208)
                     /locus_tag="LTU54_000972"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL8295703.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            34459..34728
                     /gene="rpsO"
                     /locus_tag="LTU54_000973"
     CDS             34459..34728
                     /gene="rpsO"
                     /locus_tag="LTU54_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBL8295704.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            34916..37042
                     /gene="pnp"
                     /locus_tag="LTU54_000974"
     CDS             34916..37042
                     /gene="pnp"
                     /locus_tag="LTU54_000974"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBL8295705.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            37552..37818
                     /locus_tag="LTU54_000975"
     CDS             37552..37818
                     /locus_tag="LTU54_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL8295706.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            37995..39953
                     /locus_tag="LTU54_000976"
     CDS             37995..39953
                     /locus_tag="LTU54_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HBL8295707.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            40307..41608
                     /locus_tag="LTU54_000977"
     CDS             40307..41608
                     /locus_tag="LTU54_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL8295708.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(41738..43108)
                     /locus_tag="LTU54_000978"
     CDS             complement(41738..43108)
                     /locus_tag="LTU54_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBL8295709.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVNPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(43290..43613)
                     /locus_tag="LTU54_000979"
     CDS             complement(43290..43613)
                     /locus_tag="LTU54_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL8295710.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            complement(43938..44546)
                     /locus_tag="LTU54_000980"
     CDS             complement(43938..44546)
                     /locus_tag="LTU54_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HBL8295711.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            44876..45592
                     /locus_tag="LTU54_000981"
     CDS             44876..45592
                     /locus_tag="LTU54_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HBL8295712.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            45605..46300
                     /locus_tag="LTU54_000982"
     CDS             45605..46300
                     /locus_tag="LTU54_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBL8295713.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            46384..47013
                     /gene="udk"
                     /locus_tag="LTU54_000983"
     CDS             46384..47013
                     /gene="udk"
                     /locus_tag="LTU54_000983"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBL8295714.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
BASE COUNT        15392 a         8787 c         9878 g        13413 t
ORIGIN      
        1 aataaaaaaa cttggtacgc aatcgctgta ccaagttttt ttatttagct tttgattaag
       61 ccataaataa taatttatat aagaatactg ctacgacacc agcacagata ggtgctacaa
      121 ctggtaccca tgcatatccc cattgagaag aacctttatg tttcaatgga agaagtgcgt
      181 gtacaagacg tggagcaaag tcgcgggctg ggtttagtcc aggacctgta ggaccaccaa
      241 gtgaagcaac taatgtccat acaaggaaac caagtgctaa gtgagctgta ccaagattat
      301 cttggaagaa tggtgctttt gtaataccta gggcaccaaa gaataaaacg aatgaaccaa
      361 agaattcatt gacgaaacca ttgaatttag aaccaacgct gttgattgtt gagaatgtac
      421 ctaacacatg gtttacgtct tctgtttggt cgtagtaagg tttgtaacaa gctactacga
      481 tcaattgtcc aatcgctgca ccaatcaatt gtgcaatgat ataaggaaca acttcagccc
      541 aagggaatag accattagat gctaaagcaa ttgtaaatgc tgggttgatg tggttaccgc
      601 tgattccacc aaaaatcatt gctggcatca taaccccgaa tccatacccg acagcaatca
      661 gcatccagtc actgccatat ccttttgtac cttttaaatc tacgtttgct acagcaccgt
      721 tacctagaac gattagtaat gctgtaccaa tcatttccgc cgcaagacgg gtcattagtg
      781 agtaatccat aaatacctcc aaaagttata aaaatgcaaa aaaaaaagaa acatcaacac
      841 gaaaagcatc ggtgcagtgt ttcgtgtcgg aaggatttct gcagtcttaa caaatgtgaa
      901 taaaaaaatg aattccatta cttataagaa accagaccaa cagtaaaact ccaactcttt
      961 ttaaaagttc tccacaaatc accaagctta actataaata taccaaaaaa aaaacgtttt
     1021 ttttctcaaa aattgtatct tagaaacagc atgttttgaa actttcagaa aaggtttgtt
     1081 agtaaaatga aagctatacg taaggagtgt tgtttgatga aattttttca agagtttttc
     1141 attattattt tcttttctgt attaggagaa ggcatacgga tttttgctca tttacctatt
     1201 cctggaagta tcttaggtat tcttttttta tttttagcat ttgaaatgca tcttgttgat
     1261 cctgaaaaaa tcggagcaac tggtgatttt ttattgaata atctagccat tttatttgtt
     1321 cctgctggtg taggtttatt ggaatatttt gatgatattg cttcgatatg gcctgtttta
     1381 ctgggagcag tcgtcgtgtg ttccatcgtc acgatggttg cagcgggaaa aacggcagaa
     1441 ggtgtagaag ctttgctggg ctatgtccgt aagaaaaaag cagaaagggt atctgtaaac
     1501 gaaatggagg agaatcaaat tgactgagtt ttttgtgaat ccattatttg gcttaagttt
     1561 gactttagga gtgtacgtat tatctgacaa atttttgcgg aaatataaac tgcctgtttt
     1621 gaatcctctc gtattatcga ttgttgttat tatcttattt ttgcattttt ctgggatcag
     1681 ctataaagac tataatatcg ggggcaattt tttgactatg ctgattactc ctgcgacagt
     1741 ggcacttgcc atccctcttt ataaaaactt ccgtttactg aaagagaatt tttatcctgt
     1801 aatggctgcg attttagcag gaatagtcgc aaattgcttg gttagtgtag ggttcggtta
     1861 cttcttctct cttcataaag agatggtgat ctcattacta cctaaatctg taacgacggc
     1921 tatttctgtc gatttatctc atacgatggg cggggtgaat gccgtgacac ttgctattgt
     1981 tgtctctaca ggtatttttg gttcgctgat cgccactcat attttccgcc tattcaagat
     2041 cgaaagtcca gttgctcgcg gtgtggcgtt aggaagtact tctcatgcta ttggtacagc
     2101 aaaagcaatc gagattggtg aaatagaagg aatcatttcc ggacttgcga tatgcgtcaa
     2161 cggaatattg accgttttat tgctaccgtt attctttcag ccttttgctg gcttgttttg
     2221 atgcgaaaac cactatttta tgctacaata accaaaacgt tcgagcataa aaaaggaggg
     2281 aaagacgtgt atttgtcttc cgagtatctt aaacaatttg ataaagatct atattataag
     2341 attctcctcc tagaaagttt tgaggatcaa gcttggcata ccgctgcaca attagcgcaa
     2401 gttgttcagc tggatgcccg cagcgtgtcg aaatacctga acgagctgtc taaaaactat
     2461 cagcagttta gcggtaagac acaccctttg tttacaaaaa atcaccgttc tggttataac
     2521 ttttatgata cgctcgattc aattgaacat gagcgttttt tgatctatct cgtgcaaagt
     2581 acgctgaaat tccagttgct gcacgacatt ttttttgaag aattccatac aatgtaccaa
     2641 tttgcgcaaa agcattacat tagtgaatct actgctcatc gaaaaatcaa tgaatggaaa
     2701 cagcaactgc aaacttacgg aattcggctg caacgaggta catatatcgc tcaaggcgaa
     2761 gaagaaatca ttcgacttta cttgcatatg acattctggc agttgtttcg agggaaaatc
     2821 tggccatttg aaacgatttc tcaaatggac gtaaagaata tggcagaaca tatcatggct
     2881 ttttttaatg ttcgtctgaa cgagatcaaa aaaagacgat tggaatatat gctaggtgcc
     2941 ttttttttaa gaaaatcaca gaagcattat gttgtattga atgagaaaaa aagacggctg
     3001 atttctgaca atctgttgtt ccagcgattt tgccaggtca tggagccggt atttcctaat
     3061 tattttcaag tagaagacga gttaggcgca ttatttcttg tactgatgac tcgtgaagaa
     3121 tattatagtg atcctaagat acggaagaaa atttttgatt ttcatcaaca agcaaaaaca
     3181 ccgcccttta cagccctttc agaagcaaaa gccgctcttt ctctttatca ggaagagcag
     3241 gggctgccag cagaaaatct aaccttcgag gcagaaaact atttattcag cagtcatttt
     3301 ttcgcctatc tttttcctaa tgcgaaagaa acgattgatg gcaatagtag tgattttatt
     3361 aatcatttag ttattgaaaa caaggaattg aaacaatggc tcgtacattt ttttgaaagt
     3421 agacacaaac atcccaatca tctggcattt aaaaatcatg cgtttttgat ggggcggtat
     3481 ctgactgtgt ttaaaacatt aggagccttt actccacagc ttccaaagat cacgatcctt
     3541 ttaatgacag atttcccatt atttgaagag caactgttag aggagggatt acggacattt
     3601 ttccgtaatg agtatcagct gatttttctg ccaacagatt atcgggggag agaagttgat
     3661 cttctgattt cgacaagtaa agtccaccgc aaaccatggg cggatcttga ctattttatt
     3721 gtgactgaag aactaaaatt aattgattac atccaactat ctcaaaaatt tgaaatgatt
     3781 caaaaacaaa aactattaaa acaataacaa agaaggagaa aaaagatgac atttgaagaa
     3841 attttaccag aattgaaaaa aggtgcaaag attattcgta aaggatggag tggatttgaa
     3901 ctttatgttg ttctcgtatc cgatgatgaa tttgatggct gtccggtgac gccgtactta
     3961 ttgatcaaaa caagtgacga aggtttttcc agcttcgctc ctacagtttg tgatatttta
     4021 gcagatgatt gggcgatcgt agaatgaaaa accagtatcc agatctagct aaacaggttg
     4081 tatttatcac aggagctgct tctggaattg gtgctgcgca agcccgagcg tttcttgaac
     4141 aagatgcttt tgtatttgga ttagatcaaa agttcggtaa aatggaagaa atttctttgc
     4201 gatttcctga aacttttgcc tacgcactag gtgatgttcg acaaatgaaa gaactccaac
     4261 aagcggtatc gaaatgccaa aatcattttg gtgaagttac gattttactg aatacagcgg
     4321 ggatattgga tgcctacaaa cccttgatgg aaactgacga atcgctgtgg gatctgattt
     4381 atgagacgaa tgtgaaaagc atgtatcagc tgacaaaaat catccttcct gatatgatcc
     4441 gccaaaaaag cggaacgatc atcaatatgg cttcgattgc tggcttgatt gctggtggag
     4501 gtgggatcgc ttatacaagc gcgaaacatg cgattgtcgg atttaccaaa caactcgctc
     4561 tggattacgc aggacagggt atccgagtaa aaggaattgc tccaggagcc atccaaacac
     4621 caatgaatgc ggctgatttt gaagggaaag gtgagatggc agagtgggtg gcaaatgaaa
     4681 caccagtcaa gcgatgggca cagccggaag aagtagctga gctgacgtta tttcttgcct
     4741 ctcctcaagc aagctacata caaggaacga tcatcccaat cgacggcgga tggttgttaa
     4801 aataaccttg tgtttttttc aagcttctgt atgataaaac taaagagaga aacaacggtt
     4861 gatgataaaa aaagtcgatg actgttcccg acattcgatg ccgaacagcc agttcaaaaa
     4921 attcttttta ataatcgtta atattcgaaa ctaagcggct tttcacgacc tcttgatata
     4981 aacgatattc aaaagcttgc aactgcggta acaagtccag ccaaatgaaa aatataagga
     5041 gctattttta tttgaccatt cttattactt gttgcagggc agctttttca ctaccttctg
     5101 cgacacttca gttggaattt tcttcccatc ctttggtcgc ggtcattacc aagataaaat
     5161 tatcttgcaa aggagaaata gaacatggaa aaaaataata cccgttcagc caaacgctgg
     5221 acaacgacag atgtgactcg gatggcgcta gtcactggtt tatatgtggc agtgacgctg
     5281 gttttgtctg tctttagctt tggagtgatt cagattcgtc tctcggagat gttcaactat
     5341 ctgtccttgt ataacaaaag atacatcgtc gcggtaacag cgggagtagc tttggctaat
     5401 attgcttctc ctcttggact gatagatgtt gcagtaggaa gcgtcagcac gctgcttgta
     5461 ttgcttatca actataaaat cactagtcgc atcaaaaaca tgaaaatcaa aatggccgtg
     5521 acagcatgcg tatttgcttt ttccatgttt acagtagctg gtcagctaac gattttatat
     5581 caactacctt ttttcttgaa ttggtgggtg attgctttag gagaattggc atcaatgatt
     5641 gtcggtggga taattattta ttggatcggt aaaaaagtgg atttgtcaaa ataaagatct
     5701 gtttaattta aaatagggca gcatggaaga gactgaatgt tgagtcttct tccatgctgc
     5761 ttttttgatt aatttgtatg aagaaatcga tttagccgtt ttttcatcaa gataccgata
     5821 taagctgctc cagttgcctt gattgcttcc ggcagtaaaa acggcagtag tccactggca
     5881 aatgcagttg taaaagtaag atcaccactt attttcagcc agatcgttcc gcagatcaat
     5941 gcagcaagag agcccattag attggcaaag attgcatata agtaatgagc atccgttctt
     6001 tcgatcatcc aaccagttat ccacgtttgt aagagaaatc caatcaaata tccgccagtt
     6061 ggcccaaata aaacagctat acctccagtc atccctgaaa atacaggaag accaatcaag
     6121 ccaagtaaaa gataaactcc aacagatatc aatcctagtt ttcttccgag aaaagttgct
     6181 gttaatccaa tagcaaatgt ttgccctgtc aaaggaacca aaccaaaagg aatggtcatc
     6241 tgagaaagga cagaaataat cgcggcaaat tccgcagcaa gtatcaattc tttcaatgtt
     6301 cggttcataa cgccctcttt tcgttaactt aatattttta tttggttaac gaaattgtaa
     6361 aacaaagtga ttactttgta aatagaaaat ataaaaaaag accagctatc caatagatag
     6421 cggtcttttt caagaaagct tttgaaatta taagctttgg ttgtagtatt caacgacaag
     6481 agcttcgtcg atttctggat atagttcgtc acgttctggt aaacgagtca atgaaccttc
     6541 caatttttca gcgtcaaagc ttacgaatgc tgggcgtcca acagttgctt ctacagcttc
     6601 ttttactgtt acgatgtttt gtgatttttc acgaactgag atcacttgac ccacttctac
     6661 gtggtatgaa gggatatcaa cgcgtttgcc atcaactaaa atgtgaccat ggttaactaa
     6721 ttgacgtgct tgacgacgag tcgttgcaag tcctaaacgg taaacaacat tgtctaaacg
     6781 ttgttctagt aagatcatga agttaacacc atgtttacct tctttgattt tgctagcttt
     6841 tacgaacaag ttacggaatt gacgttcgtt cattccgtac atgtgacgaa gtttttgttt
     6901 ttcagttaat tgcatgccgt attctgattg tttgccacgg ctattaggtc catgttgacc
     6961 tggtgcgtaa gggcgacgtg ctaattcttt tcctgtgcct gacaatgaga tgcctaagcg
     7021 acgagaaatt ttccatgatg gtcctgtata acgagacatt aaaatttcct ccaatgaata
     7081 taagtttttg gagtaaaata atccgttgaa aaattcataa tcatatagtt tgttcttcaa
     7141 tcttcacctt tgcagccgcg gttacacaat cgaacccgag acggggcaac aaactaggga
     7201 tgagttatta tttttctgct gcattatttt acacaaatta cagtatatga tagatcatcc
     7261 acgaatgtca agataattag tacataaaaa cgctttacaa acctaggctg tctttataaa
     7321 atgagagggt atgtgcaaat ggaaaaaagc gaaggaagtt taaggagaaa atcaatgaat
     7381 ccggtatcac cattatttga gaaaatcgat gcttcaattg aaaagaaggc gaatcttttt
     7441 gaaaacagct tttcacgtta cgctgttcga gcggttctcg cctgtttgtt tttgacttta
     7501 ggaacggctg tggcatttgc gatcgcgatg aagggggaag atatcgcgca tggtctagga
     7561 aagattttat acgcatttat gttcagctgg ggtctggtca tgattctata catgaacgcc
     7621 gaattaggaa catcgaatat gctttatatg accgtgggtg tttttcggca gagacttgct
     7681 ttaagtaagg cagccaagat tctatttgcc tgtatttttt tcaatatgat tggcggtata
     7741 ttatgtggat ttctaatttc tatgactacg ccatttcaag atttaacgtt ggacaacttt
     7801 atgttagaat caatagcagt aaaactagca aaaccttcag caacgatttt ggtggaaggg
     7861 atgtttgcga atatcgttgt caataccgct gtactgatca gcatgcggat gaaagaggat
     7921 gtcggaaaag tgatcacagt cgtattcatc attttcattt ttgctttttt aggatatgaa
     7981 cacgtgatag cgaattttcc tgcctttacg cttacttact ttgcttctca cggacagatg
     8041 gatggaatga cagtcagcaa tgtgttacat aatttgtttt ttgctttggc aggaaattat
     8101 attggtggcg gtttagtaat gggactggga tatgcttggc tgaatcagtc aaaatcatcc
     8161 tatgtggact aaaaagtcgt tcattaaagg ggaagtgcca tggaagtttt agtttttcca
     8221 aaaaatgagg aaaaagcgtt acaaatggaa aagtacatga aaaatctttt ttcctttgct
     8281 ggagtaccta agccggaacg cgctctttta caacaaccgt tactggatga gagcaaaaat
     8341 ttatctattt ctgaagtttt agcttttgtt cattattatt atgaacaaat cgaaagagag
     8401 tatcagtatg tagcgatcga tttagtccag aaaaacatca aacggcttac atttgaagac
     8461 ttagtcgtat tgttgccact agttcagaaa aaagaatggt gggacagcat cgactcttgg
     8521 agaaaagtat acagcacatg gtgtaaagcc tacccagagc agcgtgaaga agtctttcaa
     8581 ttgtttgaaa aaaaggaaga tttttggcta cgtcgaatag caatcacgtt gcaattaggt
     8641 tataaacagc aaacagacac aggactattg atccgagcaa tagacgaaga tcgtcaaaca
     8701 aaggaattct ttatccaaaa agcaattggt tgggcattac gcgattatag taagaccgat
     8761 gccgattggg taaaaagaca gctgacaaca gatttgagta agttagctgt tcgagaggca
     8821 agtaagtatt tataagaaag cgagacgaca agagatgaga cgcaaattat ttgcttttga
     8881 tattgatgga acgttgttag attcaaacaa acaagcttta gatagcacaa gagaagcatt
     8941 ggaaaaatta cgcaaagccg gtcatctggt cacgattgct acagggcgga gccgatttca
     9001 tgcgcaagag atcattcgcg atcttgattt tacgaactac attttatgta atggagcggc
     9061 cggtttttta gaccatgaac aagtatataa gaatttacta gatcgcgacc agttggatgc
     9121 gttcgttcaa gaagcgcatc atgagcagat cgatacagct tttgtcagct tagacaatat
     9181 caagcgttct tcttccaatg atgtaacggt gatggaagaa gcgatgcatt ctttcggtgc
     9241 tgtcttgcct gaattagatc agttttttgc agaggaacaa gaaatatacc aagcgttagc
     9301 atttttcgat caaacatatg atgggaagtt ctcttcatat gataaaattc gatttgtccg
     9361 ctggcatgag aacagtgtag atgtcgtacc taaaaatggt tctaaagcag cgacaattct
     9421 gcacttagca gaacaagtag gcatccctca tgaagacatc atcagctttg gcgatggaca
     9481 aaacgaccgt gaaatgttgc gcatgtcagg aattgggata gctatgggga atgctgtgcc
     9541 agaagtacaa gcagaagcta agatggtcac tgatacaaac gatcaggatg gaatttggaa
     9601 ggcattgaaa gagttaagcg tgatttaaaa gaggttgtga aaaagctgtt ctgcatcaag
     9661 taataagccc agccaaacaa aaatagctcc tcctattttt cgtttttctg ggcttattac
     9721 cgcagatgca agctttcgaa caccgtttaa ggaagaggtc gtgacagaag tggtcagttt
     9781 caagaaataa gaagggattt acgaaaattg cttctcaaat ttttgtaaaa ttacggctta
     9841 tttctgaaga agtagatgaa acaacgttta ttcgggaaat gggactgtga caagatgttt
     9901 gtcatagtcc cattttttta ttttattttt caaaactaag aaataccagg aaagagttcc
     9961 ttttgtttga ccttttcttt cttattgaaa acaactataa tatagagtga tgttaaaaga
    10021 gaagaggaat ttagtgtgcg ggtattgttt attggtgatg tagtaggttc aatgggacga
    10081 gagatgatta ctgaatatct tcctagattg aagaagaagt accgtccgca agtaacgatt
    10141 gtaaatggtg agaatgccgc ttctggacgt ggaatcacag aaaaaatcta taaaaaattt
    10201 ttgcaagacg gcgtagatgt cgtgacgatg gggaatcata cgtgggacaa ccgcggaatc
    10261 tttgagtttg taaatgaagc taaaaagatg gttcgtccgg caaattttcc ggaagggacg
    10321 cctggacaag ggatggtctt tgtcaaagtg aaccaagtcg aactggcagt gatcaacatg
    10381 caggctcgtt cttttatggt ggacttggat gatccttttc gtaaaatgaa tgagctggta
    10441 gaagaagcca gaaaacgtac gccgatcatt tttgtagatt ttcatggaga aacaacaagt
    10501 gaaaaacaag caatgggctg gtttttagat gggaaagttt cagcagtggt cggtacacat
    10561 acacacgttc agacaaatga tgcgcgaatt ttgccaagag gaacagcgta tctgacagac
    10621 gttgggatga caggtcccta tgatggtatt ttgggtatgc gcagagaacc tgtaatcgaa
    10681 aagtttctga ctgcattacc aaaacgtttt gaagttgtgg aacaaggccg atcgatttta
    10741 agcggctgta tcttggaatt ggatgatacg acaggtcatg caaaagaaat ccagctgatt
    10801 cagatcaacg atgatcgacc gttcatggag tagaaagaga ggcagttttt tgcagcagga
    10861 gccggaaatt caaaaagaag taaggaaact gacaaaactt ttaagagaga atgaaacgat
    10921 cattcgctac aaagaacttg aagaaaagat ccagcaaaac cagtatttag ctgagttgag
    10981 agaaaaaatc aaacaagcgc aaaaagatgc tgttcatttt gcccactatg acaaaccagc
    11041 agcagaaaaa gaagcaatca aacaagcgga tcaattcatg caagaatttg atcagcatcc
    11101 gcttgttgta gcttatagaa aacagctgtt ggaagcagat gatctgcttc atcatttaac
    11161 aactatgata caagaagaaa tcaatggaca aatagaggag gaaaaacatg cctcaaaaaa
    11221 ctaaaaatac tcctatgatg gaacaatatc taagcatcaa ggcccaatac caagatgctt
    11281 ttttgttcta tcgtttagga gacttttatg aactatttta cgaagatgcg atcaaagcgt
    11341 cacagctttt ggaattaacg ttgacgagca gaaatcgaaa tgcagaagaa cctattccta
    11401 tgtgcggtgt acctcaccat gcagcacaag gatatatcga tgccttgatt gaaaaaggct
    11461 ataaagtagc gatttgcgag caggtagaag atccaaagac gactaaaggg atggtcaagc
    11521 gcgaagtgat ccagctagtc actcctggta cagtcatgga cagcaaagga ttgtcggcaa
    11581 aagacaacaa ttttttaaca gctgtagtta gtcaaggaaa tgaatttggc tttgcttatg
    11641 ccgatttaag tacgggagaa ttgaagacgg ctcggttgca tgatgaagaa gctgttttaa
    11701 atgaagcctc tgcattgcag acaaaagaac tcgtattagg cagtgagatc actgataccc
    11761 ttcgtgaaca gctaagcggg cgtttagggc tagtcttttc tcaacaaaat gagatggaag
    11821 aaaacgcgga atttagcttt ttgacaagcg aactagtcga tccgctggaa aaagaagcaa
    11881 ccggcaaact attgacttat ttatcggtga cacaaaagcg gagtttagct catatccaaa
    11941 aagcagtaga ataccaacca gatcattttt tgaaaatgga ttattattca aaattcaacc
    12001 tagaattaag ccaatctatt cggactggga aaaagcacgg cactcttcta tggttgttag
    12061 atgaaacaaa aacagcaatg ggtggccgtt tactaaaaca atggctagac cgtccgctta
    12121 tccaggcaaa acaaattaaa gcaagacaag aaatggtcgc ttctttgcta gacagctatt
    12181 ttgaacgtat cgatttgcag tcagcattaa cgaaagtcta tgatctggaa cgcctagcag
    12241 gtcgagtagc tttcggtaat gtcaatgggc gagacttgat ccagctgaaa acttctttag
    12301 aacaagtgcc aatgatcaga gggttgattg aaggaatcga tcaaggtcaa tggcaaagtc
    12361 tgctttcaga aatgcagccg atggaccatc tcgtccaatt gatcgaccaa gctatccaag
    12421 atgatccgcc gttacaaata acggaaggaa acatcatcaa agatggattc aatgagcagc
    12481 tcgatatgta ccgttctgct atgagaaacg gaaagaaatg gttggcagaa cttgaagcaa
    12541 aagagcgtca agaaacagga atcaagaact taaaaatcgg atttaaccgc gttttcggtt
    12601 attatataga aattacaaaa gcaaatcttg gaaatgcaga actggagaag tatgaacgaa
    12661 aacaaacttt agcaaatgca gagcgtttca tcacaccaga actaaaagaa ttggagacac
    12721 aaatcctcga agcagaagaa aaatctgttg atttagaata tcagttgttt ttagctgtga
    12781 gagaagaagt caaaaaggct atccagccgc tacaagtact tgcaaaagca atcagtgcag
    12841 tagatgtgct acaaagtttt gcaacgatca gcgaacgcta tcagtatgtt cggccagaac
    12901 ttgtcagtga caagcatcag cttctgatca aagacggacg acatcctgta gtagaaaaag
    12961 tattaggaca tcaggaatat atccctaaca gcgtagaaat ggcagaagac gaaatgatct
    13021 tgctgattac aggaccgaat atgtctggta aaagtacgta tatgcgacag ttggcattaa
    13081 ctgttctgat ggcgcaaatg ggctgtttcg ttccggcaaa acaagcagtt ttacctattt
    13141 ttgatcggat ttttacccga attggtgcaa gtgacgactt aattgctgga caaagtactt
    13201 ttatggtaga gatgatggaa gccaatcaag ccctgcgtta tgctacgcca aacagtttga
    13261 ttttgtttga cgaactaggg cgaggaacag caacctatga cggaatggcg ttggcccaag
    13321 caatcatcga atatatccat cgtcatgtac aagcaaaaac actcttttct actcattatc
    13381 atgaactgac ggtacttgag aaagaacttc cgcagcttaa aaatgtccat gttggagccg
    13441 ttgaaaaaga cggagaagtt gtcttcttgc ataaaatgat ggatggacct gcggataaaa
    13501 gctacggcat acatgtggca aagatcgcag gattgccttc caatctacta gaacgagcag
    13561 cagatatcct gcatacgcta gaaaatggcg ataacggaca ccatactgtt ccagctgaag
    13621 tagaagagaa aactaaaaca gccaaacagg agcaaataaa accacctgca gaaaaagtaa
    13681 atcaagacca tatacaagac cgtgtaaaag aagaaaacca acagctttct ttattcagtg
    13741 aattatccga aaatgaaaca gaagtcattg aatcactgaa gcaaataaat ttactagaaa
    13801 tgacgccaat ggatgcacta aacacattat atgaattaca aaaacaactg taggaagaag
    13861 gcggatatcg atggccaaaa tccaagaatt atctgaacgt ttagctaatc agatcgctgc
    13921 gggagaagta gtggagcgac cagcttccgt tgtgaaagaa ttagtggaaa atgcaattga
    13981 cgccggcagt actcagattg atatcttagt tgaagaagcg ggactgcgga cgatccaagt
    14041 gattgataac ggagaaggca ttttggcaga cgacgtagag aatgccttca agcggcatgc
    14101 gacaagtaag atccataatc gcgacgatct ttttcgcata cgaacgctgg gctttcgagg
    14161 ggaagctttg ccaagtattg cctctgtttc tgaaatgact ctggaaacgg cgaatcagga
    14221 agaaaaacaa ggcacctatc tgtcgctgaa aggcggagaa gttattgagc atcgaccagc
    14281 accactgcgt caaggaacga agattaccgt cagcaatctg tttttcaata cgccagcccg
    14341 cttgaaatac gtgaaaacac tgcacacaga attagcgaac atcggggaca tcgtcaatcg
    14401 actggcattg agtcatccat cgattgcttt tcgtttagta catgatggaa acaaaatgct
    14461 ggcaactgct ggaaatggcg atctgaaaca aacaattgcg ggaatctacg gattatctac
    14521 cgcaaaaaaa atgctgaaaa tcgaaggaaa agatctagat tttgagttat ttggctatgt
    14581 ttcattgcct gaggtgactc gtgccagtag gaattactta tcgacgatca tcaatggacg
    14641 ttttatcaag aacttttcct tgaataaagc aatcgttgca ggttatgggt caaaattaat
    14701 ggtcggtcgt ttccctattg ctgtattgga aatcaaaatg gacccgcttt tagtagatgt
    14761 aaatgttcat ccgacgaagc aggaagtccg gctgtcgaag gaaaaagaac taacagagct
    14821 aattagtaac gccattcaaa aagcattgtc tcaagaaaat ctgattccaa gtgcagcgga
    14881 taatctacgg tttaagaaaa aagtacctga tcagcctaaa acagaacaaa tcgaaatcga
    14941 tttgtcctac gcttttgaag aagaaaaacc taaaaagcca agtagtttga attttgatcg
    15001 agagacaggt acctttttcg tagaagaact aaagaacaca tttgttgaca gaattgttga
    15061 taagtctgtg gaaaatgttg aaaactattc tggtttttct gaagaaaaca ccacttatcc
    15121 agaaaaaaca gcgaaaacgg tggataagtt tggtgaaaat gttgataact ttgtagaaaa
    15181 aacagtggaa aaatctgaaa agaaagaatt tcccattgtg gataacatgg agaaaacaaa
    15241 tgaagaacaa gcagataaat ccgtagaaga ggatgaaaag atttacgagg aagaattgac
    15301 actccatcct gaatttgatt tcactcaagg aaacgacaaa acattagaac aagccatcaa
    15361 gaaattagaa acggaaaaac cagtacaacg ttttcctcag ttggaatatt ttggacagat
    15421 gcacggaacg tatctttttg cccaaagtag cgatgggttg tacatcattg atcagcatgc
    15481 tgcccaagag cgcattaagt atgaatattt ccgggaaaaa attggggaag tatctgatga
    15541 tctgcaagaa ttgcttgttc catttgtgtt agactatcca aacagtgatg ctttgaaatt
    15601 aaaagagcaa aaacacttgc tagaagatgc agggatttat ttagaagaat ttggtcaaaa
    15661 cagctttatt gtacgggccc atcctacttg gtatcccaat ggagaagaag aatcgattat
    15721 tcgggagatg atcgatatgc tgctgacaac tggaagtgtg agtgtaaaaa aattccgtga
    15781 agcaacagca atcatgatga gttgcaaacg atcgattaaa gccaaccatt atttgaatga
    15841 agcacaagca cgtgtgctgc tgaaggatct ggcacgatgc gaaaatcctt tcaattgtcc
    15901 ccatggccgt ccagtattga ttcactttac caattcggat atggaaagga tgttcaaacg
    15961 aatccaagac ccgcattaag tcaaagagaa gagggaaaac atgaagattg tattagcatc
    16021 ccaatcacca agaagaaaag agttattggc tcatatcgtt tcagaattcg agattgaacc
    16081 tgctgatatc gatgaaacgc catttccaga cgaacagccc gtcgattatg ttcgtagaat
    16141 ggctgaagaa aaagcacgaa ctgtctggga acaagatgaa aaagaagacc atttagtgat
    16201 tgccagtgac acgactgttg ttctcaatca agagatcatg ggtaaaccag aagatctggc
    16261 tgaggctgaa ttcatgctga aaaaactaag cggggaaaca catactgtat atacagctgt
    16321 tgttttaaaa acaaaggaac gagaagaacg gatactagca gaagcacatg tcacttttta
    16381 tcctttgaca gacgaagaga tcaaacgcta cttggagacg ggggattatg cggataaggc
    16441 aggggcatac ggcatccaaa atgctgcgag tgtattcgtc aaagaaattt ctggagatta
    16501 ttacagtatc gtaggatttc cgattggtgc tgtcaatcag gcactgaaaa cattcattta
    16561 aacaaaaaag cggactatct gattttcgat tgaaaatcca tgatagcccg ttttttttat
    16621 tgaaaagatt acttatctac tctgactaag attttacttc agctttaccg actgcttcgc
    16681 ccgggcgaac aagtcctgat tggtcaagag aaagtcctgc aactttttca ttattggtca
    16741 aagcaacaat aatgtcagtt ttcttgccag cttgttctat ttctggcaaa tccatttcag
    16801 caatcagggt ctctggtgtg acaaggtatc cttctttcac gaatacattg aaaggaacac
    16861 cctttagttc aacagtatct aatcccatgt gaacaagtac ttccagcccc tcattagaaa
    16921 gaataccaat ggcatgtttt gtttcaaaga cactcgtcac tttaccgttt actggtgcat
    16981 agactttgcc gtttgacggt tcgacagcat aaccgtcacc catcattttt tgtgagaaaa
    17041 ctgggtcatt tacttcagtt atttccttga tggtccctgc agcgactgcg aataatgttt
    17101 cttttgttgc aactgccgta ccagttgctg aaacaggttc gaaagactgt ggcgttgctg
    17161 ttgcgccatt tgctgtttga agttgtacat cagcagcatt ttcaggaaca gcaggatcaa
    17221 tcttattcaa aattccctga cgtctaaaga tgatcgtcaa gccaaatgga atgatgacag
    17281 caatgatcat tgcgatgatg aaaggtaccc atgtttctgc ttggatagct agtattcctg
    17341 gcaaaccgcc tactccgatc gcgttagctc gaacacccat caagttagca aacatgccag
    17401 ctaatccaga gccgaccatt gcagcgacga aaggatagac gtatttcaaa ttgattccga
    17461 acatagcagg ttcagttacg cctaagtaac aagaaatcat tgctggaatc gagatttgtt
    17521 cttctttttt attcccacga tgtaagaaga tgatggctaa gactgcagag ccttgggcga
    17581 tatttgataa agcaatcatc ggccataaat tcgttgatcc gaaatctgca atcagctgca
    17641 tatcgatggc attggtcata tggtggagac cagtaatcac taaaggagca tacaggaatc
    17701 cgaatacggc actaaacaac cagctgatag aagacgtcaa tccagtattt acgatatttg
    17761 agatccagct tccgactgtc cagccgatag gacctaaaat aacgtgtgca gctaaaacag
    17821 ttggtaacaa tgaaaacaat ggaacaaaaa tcatcgagat cgactgaggg atatatttgc
    17881 ggaagaagat ttctagatac gctaacataa accctgccag catggctgga ataacttgcg
    17941 cttgataacc aatcatctgg acttgagcaa aaccaaaatc ccaaaatgga atatctgctg
    18001 cggctgtgga agctacacta taagcattta ataattgcgg agaaactaac gtaattccta
    18061 aaacgattcc taatatttgt gtagtaccca tcttttttgc aatactccaa gtgataccta
    18121 ctggaagaaa atggaaaatt gcttcaccgg gtaaccacaa aaaggcattg acaccattcc
    18181 aaaacttcga gtgttcaacg attgttccac caagactttc aaactggata ccttcaagta
    18241 catttctgaa tccaaggatc aatccaccga caatgattgc aggaatcagt ggtgtaaaga
    18301 tttctgctaa aacggcaata gcacgttgaa cgggatgaag gttttgtttt gcagctgctt
    18361 ttccttgttc tttggaaacc ccttcaactc cagatactgc aacgaagtcg ttataaaaag
    18421 tagaaacatc gtttcctatg atgacttgga actgtcctgc attcgtgaac atccctttta
    18481 cgctaggtat atcttcgatc gctttttcgt ccgcttttcc tggatcattc aggacaaaac
    18541 gcatacgagt cgcacaatga ctaactgcag caatattttc ttttccgccg atatcatgta
    18601 acagcttctc ggcatctgct tgatattttg ccatatcaat aaaccccttt ctcttaactt
    18661 gtctatgcaa gttgtttctt catttactat actttttttc ggagagaatt gcaagcgttt
    18721 tattaaaaag tacgagagaa aatgatatcg ttttaaaaag cttaaaataa gcttttctta
    18781 aaaataggaa cattgaaata caaaaagagc tgtagtatag tgaagaaatg atggaagaac
    18841 gagtgaggga aaaaacatga ataagttcaa tgaaattttt ttagatttgg aacagaaaat
    18901 tttagcgaaa gaatatcctc cgcattcgct tttacccagt gagaaccaat tgatcaaaat
    18961 ttatggtgtt tcacgagaaa caattagaaa agcgctgaat cttttgacta cggaaggtta
    19021 tattcaaaaa aaacaaggaa aaggttcgat cgtactggat cggaatcgct tcgactttcc
    19081 tatttctgga ttgactagct ataaagagct acaggagacc caacgtattc caagtgaaac
    19141 gatcatccat acgttagaag aaacagaagt gacaaaagca ttgaacgaga tcactggctg
    19201 ggagatcggt gcgcctgttt ggcatttgat acgcgaaaga aaaatcaatg gcgaagtagt
    19261 tatattagac accgattatc tattaaagga aatcgttcct attttaactc cagcacaagc
    19321 acaaggatcg atctatgaat attttgaaaa tgagctttct ttgacgattg cttacgcgca
    19381 aaaggagatc acagtagaag aagtaacgga aatcatgaaa acagcgatgg atcttcacga
    19441 tgacacttat gcagtagtag tcagaagcct cgttcatctg gaagataccc gctgtttcga
    19501 gtattctgag tcggttcacc gattagataa attccgtttc gttgagtttg cccgacgccg
    19561 aaaagtgtaa atagttcaaa agaaagcaaa ttgttggcat ctgtccactt tggcgctatg
    19621 ataggatcac aaagggggca tttttaatgg ataaaccaat aagaccagaa aaagaagaac
    19681 tgaaagaaaa gttatcaccc atggcttatg cagtcacaca agaaaatgca acagaacgac
    19741 catttagcgg gaaatatgat gatttttacg aaaaggggat ctatgtagat atcgtcagcg
    19801 gtgaaccgtt attcagctct gcagaaaaat atgatgcagg ttgtggctgg ccatcattca
    19861 gcaaaccaat aacgaaaaga caagtgcgcg aaaaagccga tttttctcat ggcatgcatc
    19921 gaatagaagt tcgaagcaaa gaagcagatt ctcatctcgg acatgtgttt accgatggac
    19981 cagtagatca aggtggtcta cgttactgca tcaattctgc ctcactgaaa ttcattccat
    20041 atgaaaagat ggatgaatta ggttatgggg aattcaaatc acttgtagaa taatggaaat
    20101 acacaaagag cggtacaata cggggattta ctcgatcgtc cgctcttttt ttattcaagc
    20161 gattgctatt tgaaagaaac gctccctaga ctcctttttt cacaaggtga aatgtgctac
    20221 aataaaggga acgtatgtgt gcaaaagaaa gaggactaga atatgtatga atatctgaca
    20281 ggtgtggtta cctttatcaa cccctattac atagtagtag aaaccaatgg tatcggctac
    20341 cagatcgcac taggaaatcc ttaccgctac agtagcaaac taaatcaaaa aataaaaatt
    20401 tatgtccatc aggtaatacg tgaagatgcg catcttctgt atggatttga ttcgctagaa
    20461 gaaaagcaac tgtttttgcg attagtaagt gtttcaggaa tcggtccgaa aagtgccctt
    20521 gcaatcatgg caagcgaaga ccactccggt ttgattcaag cagtagaatc cagtgatgtc
    20581 acttatttga cgaaatttcc aggagtaggg aaaaagacag cacaacaaat gatcttggat
    20641 ctaaaaggaa aattcggtga gctgagtatc gatacacctt ttagtctatt tgatgaagca
    20701 aacacaaaag acgctacagc tctatctgaa gcaatggaag ctctctcggc tcttggttac
    20761 agtgatcgag aaatcaaacg tgtagaaaaa caattaaaag aagtagataa tcagacaacg
    20821 gatgagtatc tacgccaagc actcaagcta atgatgaaaa aataaggagg agaccaaatg
    20881 acagatgaat acttgttatc accagaatct ggcgaaaatg aactgagttt ggaaaaatct
    20941 cttcgaccac agctgctttc tcaatatatc ggacagagaa aagtcaaaca agaacttcag
    21001 atttacatcg aagctgccag aaatcgggaa gaacctttag atcatgttct gttgtacggg
    21061 cctcctggat taggaaaaac gacgatggcc atggtcattg ctaatgagat gaccgtaaat
    21121 attcgtacga cgagtggccc tgctatcgaa cgtccgggtg acttagtagc aatcttgaat
    21181 gaactggaag caggggatgt cctgtttatc gatgagatcc atcgattacc tagagtggta
    21241 gaagagatgc tttattcagc tatggaagat ttctatgtag atatcatggt aggacaaggg
    21301 ccaaccgcac atccggttca tttccctctt cctcccttta ctttgattgg cgcgacgaca
    21361 cgggcaggta tgctttctgc acctttacga gatcgatttg gcatcatttc tcatatggaa
    21421 tattatgacg aacaggatct gcaggagatc gttttacgtt cttcggatat ttttcaaaca
    21481 gagatcattg aagaaggggc attggaaatt gctcgccgat cacgaggaac gcctagaatc
    21541 gctaatcgct tgctcaaacg ggttcgtgat tttgcgcaag tccaaggaga cgggaaaatc
    21601 gatcgtccta tagcggatca ggcacttact ttgctacagg tcgatcaggc tggattggat
    21661 tatgttgacc agaaattgtt gcgaacaatg atcgagttat atggcggcgg accagtaggg
    21721 ttaagtacgc tttcagtaaa cataggagaa gagcgtgaga cggtagaaga tatgtatgaa
    21781 ccgtatctga tccaaaaagg atttttaaag cgtacaccta gagggcgaat tgctactgct
    21841 tatgcctatg aacattttgg ttacgactat catatatgat ttttgctttt agattggagg
    21901 aaaatgaatg agtgatacaa cacgtacgaa acaattgatc aaaaacgtat tgattgattt
    21961 gtgccaaacg caatcttttc gaaaaatcag cgtccaaaaa atcgcaaacg aagcaaaaat
    22021 caatcgacaa acattttatt atcattttac agataaatac gatttgcttc gttgggtcta
    22081 ttgtgaggat gcattgcatt atttagatcc acaatgtctt tcacttgata attgggaaga
    22141 gcaagcattg aaaatgctga aagcaattgc tgagaagaaa gaattttatg caaatactgt
    22201 gctttctgac ccagaaattt tacaaaaaga gttttctcaa ctgatccaaa aatcatttat
    22261 caagattttt gaacaaatgg atgaagaaag acaactttct gcaagagaca aactatttta
    22321 tgctcgtttt ttttcttacg gttgcagtgg tgtattagtc aattggatca cgaatgaata
    22381 tgaagaaaca ccgatggaaa tcgctatgca gcttttccgt ttagccaagg atacggaatt
    22441 atattcttat cgtctttatg caaaagaaga gttggaagaa cagtaataga aaaatttttt
    22501 gttggaaaag caataatcgc taaaaggaga tggggaaatg acaaaaattt tatttgtttg
    22561 tttgggaaat atttgccgtt caccgatggc agaaggcttg ctgagaaaac gaatagcatt
    22621 agaaggaagg gaagcagaat tttttgtgga ttctgctgct acaagcacgt atgaagttgg
    22681 aaagacaccg caccctggaa cgaaaaaaat attaaaccaa gaaaaagtag atatgacgaa
    22741 tatggttgct cggcaaatca caccccacga ttttgaaact tttgattgga tcattggcat
    22801 ggatcaagaa aatgtagaag aactgaaacg aagagcacca cgctcagcgc aggggaaaat
    22861 ccatttgttt ttaagtagca ttcctggaaa agaaaaagaa aatgttcccg acccatacta
    22921 taccaataac tttgatgaga catatcactt gatcacagaa ggcctgaatc attggtggaa
    22981 tatttggatt gatgaaaata aaataacaaa caatttgtaa agttaacgct ttctttttaa
    23041 ggcgatgtta gagcatttcc ttgttttttt gtaaaatgtt aggtaaaata aaattaatta
    23101 agtaacactt tcttagctaa aggaggagtg cattcgatga acgaagagac agtatcgcca
    23161 atgaagaagc ttacgaagtt agttactcat acttcgtatc aactttttga ttcttttgta
    23221 aatgacactg aagaacatgc taagaatgta cgattgcaag gaaagtttat tcttgagctg
    23281 caaaaagctg ctactgaaca tagtctagtt atcttacaag taagagcaaa tcgtttgagt
    23341 agcaagtatg aaacgatctc aggctgggtg gcgacaaaaa cggtcgaaaa agaccgagtg
    23401 atgatccgcc tacaaaacga agagcagcag atccggatgg ttcctgtaga acaaattgaa
    23461 aaaataacaa tcctttcacc agcaggggaa caggaacggt tatcaaaata atttatacca
    23521 gatagaaaag cacgtatgag cttgtaccat caatcattac taatcaaaaa taaccgaatg
    23581 ctttaagctg atatcagtaa aacggaacgt tttaggatga gtatcacagc atgacagcat
    23641 tgggttgatt agttgaatcg attggatggt acaagttttt ttgataaaaa aaggtcctgc
    23701 tagccgggga gctagcagga taaggagtta aaaatgaaaa agtgttgtta gggttgtttg
    23761 ttggtatgct tatataatag tcactagttg tgaatatttt gtgacgaaag ttttcgaaag
    23821 ttgttaagtt tttttgcgaa aactattttt atatcatata tatgtctaaa atctatatta
    23881 taaaagaaat gttgtttgat cagcttctct tcggtattag ttcaaaacaa aacggcaaag
    23941 tatatggagc tactttgccg ttttgtttct ctaatactca ggtcagttta tagacgctca
    24001 ataaacggtg ttcaaaagcc agtttctcgg tattaagtca aatttatgca aaaatgtgag
    24061 aagccatttt caaaaaatat ttcttaatac tcgaaactga gcggcttttt cacgacctct
    24121 caataaacag tgttcaagag acagcctatc ggcaataagg ctgaacgtct ctttcacaac
    24181 ctcttttatc aatctgctac ttcttctttc ttagcgaaac tccaaatcag tccgataaca
    24241 aaaccgataa ctgctggtac gatccacccc atccccaatt tgaaaaacgg caagtagctt
    24301 tccgcaacat ataaaagatt ttgaacaata ggtgtttgtt tgatagtttc tgggcaagcg
    24361 ttcaacccat caaaaatcga agcaatcaat gtaaaataag tagtcattcg ataaacagcc
    24421 gtacgctgct taaacaaagg accaactaac actagtaaaa tcagtgtcat tgctaatgga
    24481 tagatgaaca tcaaaacagg caacgaaaat tgaatgatat tcgtcaagcc tacgtttgcc
    24541 gctaaacaag gaaggatact agcaagtgca acaaagaatg cgtaatttct ttgcggaaac
    24601 aaatctgtga atgtttctgc aaaagctgta atcaaaccaa taccggtctt caagcaagct
    24661 aatataacga taaatgctaa taagatactg ccataagtac ctaaataatg atcagcaatc
    24721 tgagccagcg tgatcccgcc attttcactt agagcaaagc tacctaagct catagcacct
    24781 aaataagcga gcaaagtata aatgattccc atcaatataa tgctgatcgc accagatttg
    24841 atcgtgtctt tagcgatttc agcaggtttt gttacaccca tgttttggat tgttgtgaca
    24901 atgatgatcc caaatgctaa agcagcaagc gcatctaatg tgttatatcc ttgagtaaac
    24961 cctgtgaaaa aagcatgctc ttgataattt ggctgaatag gcgcttgatt tacagaacct
    25021 agtggattca aaaaagcaag gagaagcaaa gcacctaata aaaccaaaaa tgcaggattc
    25081 aggaactttc caacataatc taatatcttt gtcggttttc ttgaaaacca ccaagccagc
    25141 acaaaaaata caatactgaa aattgctaga tatagcgtct gtctgctagg atcgataaat
    25201 ggtgctaaac caatctcgaa agatgtggtt gcaaggcgag gtaaagcaaa aaacggtccg
    25261 ataactaagt aaagcaagat ggtaaaaacc atcgcatatg tttgatttat ccgttttgct
    25321 aattcatata cacctgagct tttcgataca ccaatggcaa tcacccctaa gaagggaaga
    25381 ccgataccgg taaccaagaa acctagattc gcccaaaagg tagcagcgcc tgcttcttga
    25441 cccaaatgaa cgggaaagat aagatttcca gcaccaaaaa atagaccgaa aagcatggag
    25501 cctataaaaa gatagtcttt aaatgataat ttttttgtca tacataactc ctttacggtt
    25561 tttttaaaag taacaaaatt tcgaaaaata tgcaacaatt ttcagaaaat ttcctatata
    25621 aaacgacggt tatttaataa cgtaagtgat aaaataaaag cgctgaatca tctgaggagg
    25681 aatcatcgtg acaaagtcga gattagaagc atttactgat ggagtggtag ccattgtttt
    25741 gagagttctt gtattagaga tcaagatccc taatacatct agctttcatt ctttatgggt
    25801 catccgaaat acattattag cttataccat cagctttata tttgttgcgg tgatctgggt
    25861 gaaccatcat cgtatatttc agatggcaga acgaattaat taccgagtgg tctggtcgaa
    25921 tatctttttg ctcttttggc tgactttatg tccttctgtt actagttggg tcgggcgtaa
    25981 ccctgaagct ttttggcctg agatggctta tgtcttagta tacacgatgt ggagtttttc
    26041 ttttggtatc ttgatgaggc aaatcatgaa agcaaataaa ccagattccc atgtcgtgag
    26101 agttttgtca aaggaccgtc gaagtcttat ctccatgatg attaacttat gcttgattgc
    26161 cggtgtcttt tttgtaccgt tgatcgggtt attcggacgt ttttttgtat ctggtatttg
    26221 gattttttcc tataaaaaag cagatgccta ttatcagcgg ttgtttccgg ggaaatagaa
    26281 aagaggctgg gacagaagct tttaactcca agaaataagc cgaaatttca caaaaattta
    26341 agaagcaatt ttcgtaaatt ccttcttatt tctcgaagct gaccacttct gtcacaacta
    26401 ctttcttact tcttacgttc atatttcata tgatagatta gtaatagaag caagaaccac
    26461 acaggtgtta gtaataaagc gattctcgta tcttttccta ggaacaagac gacacacaca
    26521 aaggccatgt aaagaaatac cagataatta ctaaatgggt acaacggcat tttaaatgaa
    26581 tgagcttttt tgccgaggat acttttacga tatttcatgt gcgtgtagac aagcatactc
    26641 cagataaaca agaaacaagt agtggcaata ctgcttacta gtgtgaatac ttcactaggc
    26701 atcacgtaat tcaagataac agaaatgaaa atgacgatag tagaaaatgt caaagcattc
    26761 cctggcacat gatgtgttgt taactttttg aattttttag gggcactgcc ttcttgcgct
    26821 aaagaacgaa gcatacgtcc tgtactatat attgcactgt tgcaggcaga agctgctgca
    26881 cttaatacga ctaaattgat caaggaagca gcaatcgtga tcccaatttc cgaaaatact
    26941 tctacaaatg gactgttatc tggattcaag ctgttccatg gatagattgc cataatgaca
    27001 aaaagagatc ctaagtaaaa taagatgatc ctgataggaa tattattgat agcttccggc
    27061 aacacttttt caggattttc tgtttctcca gcgactaatc caactaattc tatcccagca
    27121 aaagcaaagg ttaccatttg gaaagctaag ataaatcctt ttgcaccagt tggaaagaat
    27181 ccgccatgag accatagatt cgtgatactg gctgtgccaa tatctgtcgt atagtgggtg
    27241 aggatcatat aagctcctac gaggataaag gcaatgatcg ctacaacttt gatcaatgca
    27301 aaccaaaact ctaactcacc aaacaatgag acagccacca tattcaagca aagaagaaga
    27361 cctaatgcaa tcagttcagg aagccactga ggaacaccag gcgcccagta gcgtacatac
    27421 attccgatgg ctgttaaatc ggccattgca atcgataccc agcagaacca atacgtccag
    27481 ccagtgatga aagccgcttt tttgccaaga tattctgcaa caaaatccag aaaagaatgg
    27541 ttattggtgt ttgataacag aagttcgcct aacgcacgca taatgaaaaa acagatgcat
    27601 ccagtaataa gataggcgag caatatcgaa gggccagcta gctggataga ctttccggaa
    27661 cctaaaaaca accccgttcc aatcgctccg ccaatagaaa tcagttggac atgtctattt
    27721 ttcagtccac gctgcagttc ttgctcttca ttcattttta aatcatccta actaaaaaag
    27781 tttaattact tccatattaa aagagttttc tgaaaaacac aactaagatt ccaaaatttt
    27841 cagaaattta ccttttatga gtaattcata aaaaagaact gcggtaaaca ccgcagctct
    27901 ttgtcccaat cagctattga tcctaagcat ttaaaatacg tgaaaggaat tcttttgtac
    27961 gttcttcttt cggatggaca aagatatctt ctggtgtacc ttcttctgca atcacacctt
    28021 tatccataaa aatgacacgg tcagatacat cttttgcaaa ttccatctca tgcgtcacga
    28081 taagcattgt caagccagta tgtgctaagt ctttgatcgt attaagcact tcgccaacca
    28141 tttcagggtc taacgccgaa gtaggttcgt cgaatagcaa tacatctgga ttcattgata
    28201 atgcccgagc aattgcgaca cgctgtttct gtccaccaga taattgtgct ggtttggctt
    28261 cgatataacg atccatacca actttttcca gattttcgat tgctactttt ttggcagttt
    28321 ctttatctcg tttcaggaca gttgtttgac cagacataca gttttccaag acattcatat
    28381 tgttgaacag gttgaaggat tggaaaacca tacctagatt cgttcgatat ttaggtaaat
    28441 tatatttagg agctaataca ttttctccat tataaatgat ctcgccgcct gttggttttt
    28501 ccagtaaatt gatacagcgc aagaaagtag atttaccaga accggaagaa ccgatgatcg
    28561 tcacaacttc acctttattg actgttacgc tgatatcttt caatacttca ttttcaccga
    28621 agctttttct tagatgtttg atttcaataa ttttttcatt catgattctt cctccttatt
    28681 cctgcaaatt tgaattgtcg atttcttcaa gcttggtata agctgaagga ccatcgattt
    28741 tcttttctac cgcccgcagg attctggtga tcgtaaaggt catgactaag tagatgatcc
    28801 cgacgatcgt aaatgtttgg aagaattgga aattcgcacc ggaagcagag tttccttgga
    28861 agaacaaatc agctacgccg attacactta aaactgctgt atctttgata ttgataacaa
    28921 actcatttcc tgttgctggc aagatattcc gtaaaacttg tggaataaca acttttctca
    28981 tcgtttggcc atgggtcata ccaatggctt gcgcagcttc gaactgccct tcatctactg
    29041 caaaaatccc cccacgaacg atctctgtca tgtaagctcc agtattgaga gagacaataa
    29101 acaaagcggc aatcgttcgg ttcaatgaaa tgccaaaagc gagtgccaga ccgtagaaga
    29161 tgaccattgc ttgtaccatc atcggtgttc cgcggaatac ttcgacatag atagaaagaa
    29221 gtgcatttcc gattttttgg aagaagcgag tcagtgcatt tcctgattca ggtaccgaac
    29281 ggaagacacc aatcagtaat ccaagtgtag taccgacaac tgtcccgatc aatgcgatga
    29341 acaaggtaag tccagcacct cgtaagaaca tattgccgta ttgtttccaa atcgtagtga
    29401 aatcagaaat gatgccaggt ttttcttcac tatcagttgc agcaggctga tctttgactg
    29461 cttggtccat aattttggtt cgttcatcgt tagagatgcc agaaagaatc tggtttactt
    29521 tttggatatc cgggtcgcct ttacgcatac caacagaaat ctgaacatct tccgggtttg
    29581 tctggaagcc atctgcaccg ctgaattcaa gcatttccaa gtctttgttc acactagttg
    29641 ccgttacacc ttccggacgt tcgctgacat aaccatcgat cattccagaa gctaaagctg
    29701 ttctcattgc tgagaaatta tccattgctt gttgtttctg cacatctggt atctgatcga
    29761 tcacagtata gtggaatgta tttaactgag cagtaatctt tgctccagaa agatctgcta
    29821 aactttttgc ttttgcatat ttgctatctt tttgaactac tactactaat tgtgattcgt
    29881 aataaggatc ggtaaaatca atttcttttc gtcgttcagc agtagggctc attccggcga
    29941 tgatcgcgtc gatcttgcct gattgaaggg caggagccag accatcccat ttcgtttgga
    30001 cgatgactaa ttttttccct aaaccatctg ctactttttt agcgatttga acatcataac
    30061 cgccggcgta actattttgg ccttgaattg gcaaggcatt attctcatct gtttgttgag
    30121 accagttgaa gggtgcatac ccagcctcca tacctacgcg aaactcatcg ctcggtgttt
    30181 tttcatctgc atttgccaga gatcccacac tgaatagtga gaagacaaaa gcaaatagta
    30241 cggcaactga aaccatattt tttttcattt ttttctccct ttctgattat atgatacacg
    30301 agcggtctag atgaaatgcg gtaaagcttt atctgaaaaa ataaaaagcg accgatttat
    30361 agatataaaa aggccgctaa aattccataa ggtgataaac ctctcacaaa acatagcgca
    30421 acttagcatc actgctaaga cagtccgcaa gctcttaact tgcgtcccaa catacactcc
    30481 aagcaaaaaa gtgcacattt cggcgatcct ccctattcct gcttctccat gtttgcttca
    30541 ctccacaggg ttaatgattc tcgcgacctc tacctcattg attgaggcat ctcgtattca
    30601 gttatttata caaaaaaagt ttacgatact cgagaggctt tgtcaatcta taactgctgt
    30661 tttttgaaaa aatactgata aatatagaaa tctttacttt taccgaaaaa acaatcaaaa
    30721 tgtgaatcat ctatccattg caaacgatag tataattata cacaaaaatg tatgtaatac
    30781 aataaaaaaa gaactagaac agaaaatttc ctgtgtccag ttccatctcg atcatttaag
    30841 ctgccgcttt ctttggctca cgtcctagga tcgcttgcaa aagaacaacg acgccataaa
    30901 tggctaatat tagataaatc aaacttgagt attgggtgat tccccagccc catgttcgat
    30961 atacggctaa tgtaatggct aaaacaattg cggcaatcac gttgaataaa gcaaaagccc
    31021 aaagatttcc attttctttg cgtacaagat aaaaaacaga gaaataaacg cttaacgcta
    31081 accaggcgat acaagccaga ataacgcccc aataaataaa gaatggtaac atatcgattc
    31141 acctcacttt tatacagcag tattgtaaca cgttttcatt cttttgtcag tgaacattgt
    31201 gataaattgt tcaaatgccc ataacacttt tttattccca tttttctgac ttttcaacaa
    31261 taaatttgtt cgagtatggg ctataatgga aagtgaggtg acaaaacatg aaacaattaa
    31321 cagtagacga agcagcaaaa gagatattag cttatcagca aattacattt ttgaccggtg
    31381 caggggtatc cacaccttct ggagtgccag actatcgatc tttgtcagga atctaccacg
    31441 ggatggagca gccggaatac ttgctgagtc atcaagcaat ggtgaatgaa ccggataaat
    31501 tctatcaatt tgtcaaaaag atttatcatc ctgaagcaaa acccaatatc attcatttgg
    31561 aaatggcaca tctagcgaaa gaaaaaaatg tttgggttgt ttcccaaaac atcgacggtt
    31621 tgcacgcaaa ggctaaaagc ccgcaactcg tcaattttca tggaagtttg tatcaatgtc
    31681 attgtcgaaa atgtcaacaa acggtagact ggaaagaata tcttcacagc gacaaacatc
    31741 aagcatgcgg aggacaaata cgcccagaca tcgtgttata tggtgaaggg tttcaagatg
    31801 gagtcatgga gcaggcggct tatgccgtca gccaagcaga gctgatcgtc attgttggaa
    31861 cgagttttca agttcatccg ttttgtgatc tggtacagtt tcgccagccg tcagccaaaa
    31921 tcttagtaat caatcaaacg cctgtttatt tagcggagcc ttatacgttt attcaagaag
    31981 acggagcggt tgttttcaaa aaaatacaag aattaggagc agattatgac tgaattcaaa
    32041 accgaaaaag aaaaaatgat tgcaggcgag ctttattttg ccaatgatcc tgagctggtt
    32101 gccgaccgta tgtttgcaag aagtcaaagc caaatcatca accaagcaga atcagcggaa
    32161 ctgcgatccc aattgttgaa agagactttt ggccgtacag ggaaaaaaat ctatatggag
    32221 ccagttatca attttgatta cggatataat atatttgttg gggagaattt ttatgcaaat
    32281 ttcaattgta catttttaga tgtcagcacg attgaaatcg gtgataactg catgttcgct
    32341 ccgaatgtcc agctttacac ggcgacgcat ccactacatc cagtgaaacg taacagcggt
    32401 ctggagtacg caaaacccat taaaatcggt aataatgttt ggttaggcgg aggagttatc
    32461 gtcacgccgg gtgtcactct aggggacaac gtagtagtcg gcgcaggttc ggttgtgacc
    32521 aaatcttttc ccgacaatgt agtgattgca ggaaatcctg ctcgtatcat caagacagta
    32581 gaggaagaac taacagaaga gtctcttgaa acattacgtc ataagatcga tatgatcgat
    32641 cgagagttga ttgctttatt agaaaaaaga atggatacgg taactaaaat aggacaagtg
    32701 aaaaaagaag aaaaccaagc agtttatgat gcaaaaagag agcagcaagt attggataaa
    32761 gttgttagtc tgctgaaaaa taagggatat aaggaaacaa taacagatac gtatgttgat
    32821 ttgatgaaac actcgcgcga ataccaaaac aaaatgaagg aggaataagt gtgatcgaag
    32881 gagagacacg gcgaaaacaa atcatgcaaa ccttggcaaa tacagaaaaa ccagtgagtg
    32941 ctagcaagtt tgcgaaaact tttggagtca gccgtcagat cattgtaggc gatattgctt
    33001 tactacgagc tgcaggtaaa agtatcgtgg caacagcaag aggttatatc ctcgaaacag
    33061 aagaaaccaa tactgggcac atcagtaaaa tcgctgtcca gcatggaaaa gatcaaacag
    33121 aagaagaact gagactaatc gttgaaaatg gtggtgaaat cattgatgta attgtggaac
    33181 atccccttta cggtgagtta gcaggaactt tgcacatcaa aacgccacaa gatatcgatt
    33241 cctttatgag acgttatcaa aaaagtaatg ctactttgtt atccgaatta acaggcggca
    33301 tccatctgca tacgatccgc tacccagaaa agcatatgct gaaaaacatc aaacaaaaac
    33361 tagctgaagc aggaatcctc tacgaaggat aacatcgtag ctgctacttt ttatcctaga
    33421 aactattcta tcatctctga gataaacacc catatggaag aaataagaaa aaataagaaa
    33481 agatgcgtat ccaatacaca gaattttttg gaattgcgat ttctagatgt ctatctaaat
    33541 ttcgattagt ggagaaacat aaaaaaggga agtgcagctt ttgagcactc ccctttttta
    33601 taaagctttc ttgtagcata gtatcgaagg aacctgccaa ttctctactc gatcaccaat
    33661 acgccttctt ttagtgcaaa tggattttga tcgttgatgt ggtcatagaa catcacaccg
    33721 ttgatatggt cgatttcatg ttgaacgacg atcgattcat aattcttcaa acgaatcttg
    33781 tgtttttcac cattcatatc ataataagac acagtgattt tggcatggcg aacaacatat
    33841 cctggtactt ctcgatcaac agataaacag ccttcacctt cccctaaaca agcatcttga
    33901 acagaatggc tcaaaatttt aggattatac ataacagtac ttagagaagg tccatctgcc
    33961 tctgggtcag gacttggtac gtgaacagca atgattcttt tagaaatatc taattgagga
    34021 gcagctaagc caacaccgcc tcgtaagtgt agttcttcag ctttgattgg atcttggctg
    34081 ttttttaaga actcaagcat ctcttttcct aaagagatat cttcttcgct taatggaaga
    34141 gaaacttctt ttgcaacttc gcgtaaggtc ggatttcctt cacgaatgat atcatccatt
    34201 gtaatcatgg aatatgtacc cctttcttaa aagactgtgt ttcaagtcta ttttcgcatt
    34261 atagtttacc acaaaaatgg aaaagtagta gtaagattgt ttgaaaacta ccttttctta
    34321 atgattaact acttgatttt caacccggtt tcaggtaaag tatagctgtg tgctctaggc
    34381 acatgacctg ccacttggtt aagcgaatca ccaacgtttt actcagtcgc aggggaataa
    34441 tcgaaagagg tgaaagaaat ggcaatttca aaagaacgca agaatgaaat catcaacgaa
    34501 tacgctcgcc atgaagggga tactggttct ccagaagtac aaatcgctgt attaactgaa
    34561 gaaatcaacc acttgaatga acatgcacgc gtgcacaaaa aagaccatca ttcataccgc
    34621 ggcctaatga aaaaagttgg tcaccgccgt aacttgttag cttacttacg taaaactgac
    34681 gttcaacgtt accgcgaatt gatcaaacgt ttaggcttgc gtcgttaatt ttagtcaaaa
    34741 agagtgggat tcacatgaat ctcacttttt ttactcgtat atcaattaag taaaattccg
    34801 tgatcaagct agatggactc ctactaacca aatgaaagct gctcaaaaga cggctttcgg
    34861 cagttttcat ttgtttagta gcgtcttgct ttcacgagaa ggaaaaggag aagttatgtc
    34921 agaaaaacaa gtattcaaaa cgacatgggg cggccgtccc ttacaaatcg aagtcggtca
    34981 gctagcaaaa caagcaaacg gagccgtttt agtccgttat ggagatacag tagtcttaag
    35041 tgcagctgtg gcctctaaag aagcaaaaga tacagacttt ttcccattaa cgatcaatta
    35101 tgaagaaaaa atgtatgcag cagggaaaat ccctggaggt ttcatcaaac gagaaggccg
    35161 tccaagtacg gaagcaacat tgactgcccg tttgatcgac cgtccaattc gcccgatgtt
    35221 tgcagaaggc ttccgtaatg aagtccaagt aacgaacatc gtgatgagtg tagaaacaga
    35281 ctgctcgcca gctatggcag caatgctagg ttcttcttta gcactatcaa tctcagatat
    35341 cccatttgac ggcccaatcg caggcgtaga agttggtcgt gtaaatggcg agtacgtctt
    35401 gaatccaaca gtagaacaag cagaacaaac agatatcgaa ttgactgttg ccggaacgaa
    35461 acaagccatc aatatggttg aaagcggcgc gaaagaagtt tcagaagaag acatgcttgg
    35521 tgcattgctg tttggttttg acgctatcaa agagttagtt gctttccaag aagaaatcgt
    35581 tcaagcagta gggaaagaaa aaatggaagt gactttactg caagttgatg aagtattgaa
    35641 aaaagaaatc tttgatgctt cttacgctac aatgaaagca gcagtaatga ctgaagaaaa
    35701 acttgctcgc gaagataata tcgagcaggt gaagatcgat atccgcgaag cttatgctga
    35761 gaaatttgca ggtcatgaag atgaagatca cttgttgaaa gaagttaaac aaatcacaga
    35821 agatcttgaa aaagatgtcg tacgtgaatt gatcaccatc gataagatcc gtcctgacgg
    35881 gcgtaaattg gatgaaatcc gtccattgtc atcagaggtc agcttattgc cacgtgtaca
    35941 tggatcaggt ttgttcacac gaggacaaac acaagcttta tcagcttgta cactcgcacc
    36001 tctaggagag caccaaatca ttgatggttt aggggtggaa gtcagcaaac gattcatcca
    36061 ccactacaat ttccctcaat tttctgttgg ttctacagga cgtgcaggct ctccagggcg
    36121 tcgggaaatc ggacatggcg cattaggtga acgtgcgtta gcacaagtaa tcccttcaga
    36181 agaagaattt ccttatacga tccgtttagt agcagaagta ttggaatcaa atggttcttc
    36241 ttcccaagca agtatctgtg ctggcacatt agcattgatg gatgcaggtg taccaatcaa
    36301 agcgccagtc gccggtattg cgatgggact tgtttctgat ggtgaaaact atacgatcct
    36361 aactgatatc caagggctgg aagatcatct tggcgatatg gactttaaag ttgccggtac
    36421 aaaagatggt atcactgctt tacagatgga tatcaagatt caaggaatca cagaacaaat
    36481 cctaactgaa gcattaactc aagcaaaaca agcacgtatg gaaatcttag aagaattgac
    36541 ttcaacgatt gctgctccac gtgaagaatt aagccaatat gcaccaaaaa tcgaaatgat
    36601 ccagatcgaa cctgctaaaa tcaaagatgt tatcggtaaa ggcggcgata cgatcaacgg
    36661 aatcatcgat gaaactggtg tgaaaatcga tatcgatcaa gatggaaaag taagtattgc
    36721 ttcagctgat gcagaaatga tcaaaaaagc tatcaagatc atcgaagatt tgaccaaaga
    36781 agtcaaagtt ggtgaagttt atctagggaa agttgtccgt atcgaaaaat tcggtgcatt
    36841 cgtcaacttg atcaaaggaa aagatggact tgtccatatt tcccaattag caaacgatcg
    36901 tgtcaataaa gtcgaagatg ttgttaaact aggtgatgag atccttgtga aagtaactga
    36961 aatcgataaa caaggacgcg taaatctgtc aagaaaagca atgttgaacg aagatggttc
    37021 aacgaaaaat gaagaaaaat aatttaattc ataggaataa tgtacttgtg atcgtgaagg
    37081 tgacaggggg acaataatta ttcgaaaaaa ggggactgtg acaaaagccg tgtcacagtc
    37141 cttttttttc gaataaacgg tgttcaaaag cttgcatctg cggtaataag tccagccaag
    37201 ttaaaaacat taggagctgt ttttatttgg ctgttcttat tacttgatgc agaacagctt
    37261 ttttacaacc tctccaataa acgatgttca atcagctgtc cctcgatatt agctcaaaaa
    37321 acggcaaaat atgaggaact atttttccgt ttgtttctct aatactcagg tcaaaccgct
    37381 gatttcacaa cctctgattc acggtgttcc agaagcagct tctacggaaa taagctgaac
    37441 acttttgtta caacttcttt ttttctggag tttcttggcg catatttttg gagtccttct
    37501 gaataaagcg taaaataaaa gtgagaaata tggataaagg agggaaagga gatgctgaat
    37561 tttttatggt cgttatttat cggcggtata ttaggtttga tagctggcgc aattttagga
    37621 aaggaagtcc cgggaggcgt atctggaaat atcattatcg gttttttagg tagttggctc
    37681 ggcgaatttt tactaggacc actgggaccg gaactcggcg gtttttattt gataccagca
    37741 ttattgggtg caattttttg ccttgctctt tattcatttg tggcaagacg tatcagagaa
    37801 cgtccaaaga aagaataatt ttattcaaat aagttgacac ttcagtgtgt tacttggata
    37861 attagaagca atttaggaaa gcgggtgtca tggataagaa tatcatctat cattcaagct
    37921 tacctaaagg atatccatat aacaagaaat gagggaaaaa cgtggcaaca acgcacaata
    37981 ccaaaaagat atcaatgact ggcctgcttg tagcgatggg agtcgtctac ggagacatcg
    38041 ggacaagtcc gctttacgtt atgaaatcga ttgttgaaga taatggcgga ttgtcaggaa
    38101 tcaatcctga ttttgttatc ggatcagtct cattgatttt ttggacattg actctactga
    38161 caacaatcaa atatgtcgtg attgcattga atgcagataa ccatggtgaa ggtggaattt
    38221 tctcactcta cacacttgtc cgaaaaggtg ggaaatattt gatcattcct gcaatgatcg
    38281 gaggagctgc cttactagca gatggggtat tgacccctgc tgtgacggta acgactgcga
    38341 ttgaaggctt gcgaggaatt ccgcagtttt ttgaccggtt cggtaataac cagaacgtga
    38401 tcgtcattat cactttagtt attatttttg ttttattcat ggtccaacgc tttggtacag
    38461 atattgtcgg aaaagcattc ggaccaatca tgttcgcttg gtttactttt ttaggcgtaa
    38521 tgggattaat gaacttcagt caagactgga cagttatccg tgcactgaat ccttattatg
    38581 ctatccagct gctttttagt gcagataaca aattaggctt gttcattctt ggaaatattt
    38641 tcctggctac gacaggtgca gaggcgcttt attccgatct tggacatgtc ggcaaacgaa
    38701 atattcgtct gagctggcct tacgtcaaaa tttgtttggt tatcaattat tttgggcagg
    38761 ctgcgtggtt gttgaatgtt tatcaaaacc ctgaagcaca gcaaatcaag aacctgaatc
    38821 cattcttcca aatgatgcct caaagttgga tcgtgatcgg cgttgctttt gcaacagtcg
    38881 ctgcagtcat tgcttcgcaa gcattgatta caggttcgtt tacgctagtt tcagaagcaa
    38941 tcaagttgaa actgctgcct cggttaaaga tcatgtatcc tgggaacagt atcgggcaaa
    39001 tgtatatccc agcagtcaat ctgattttat ggatcgcctg ttctctagtg gttgtcacat
    39061 tccgaacgtc tcatcatatg gaagctgctt acggtctatc gatcactgtg acgatgttga
    39121 tgacaacagc actgctttac ttttatctta tccaaaatgg gcattctcgt tggcttgcat
    39181 atttagttac ctttttcttc ggtgcgatag aagtcgtttt ctttatctca agtgttgtca
    39241 aattcttccg aggcggtttt gtagccgttc tgattggttt gatcatctta gtcgtgatgt
    39301 ttatttggga acaagggaac attattcgcg aatcgtcaac cgaggatgtg tctttgagag
    39361 attatatccc gcagcttaga gaattacgtg atgatacttc tttaccaatc tatcagacaa
    39421 atgtggtctt cttggttcct gatatggctg aagataaagt tggccgacag ttcatctatt
    39481 cgattttaga taaacggcct aaaagagcac gagtctattg gtttgttcat gtggaagtga
    39541 cggatgagcc atatacgaaa gaatatcaag tagatatgat ggacactgat tttattgtcc
    39601 aagtgaatct gttcttaggt ttcagggtcc aacaggaaat caacgtatat gttcgccaag
    39661 tcatccatga tttgatgaaa caaggccgtt tgccaaaaca gccacaaaga tactctctga
    39721 caccagggcg agaagttggc gatttccaat ttgtcctgat tgatgaagta gtttccaatg
    39781 tgacaactct aggaaaatgg caacgtcaaa tcatgcaggc aaaactggca atcaaaaaaa
    39841 tcgctacctc gccagaaaca tggtttgggc tagagtacag cgaagtaaaa catgaaactg
    39901 ttccgcttat cattggaaat actcggaaaa catggttgaa agagcggttg tgaaagtagc
    39961 gctttgacct gaactaatgt cgaatgtcag ctacttgaac accgtttatc agaggtcatg
    40021 aaaaagctgt actgcaacaa gcagtaagaa cgagcagata aaaacagctt ctcatgtttt
    40081 tcatctgttt gggcttaatg ccgtagatgc aagcttttga ataccgtttt tatcagaggt
    40141 catgaaaaag ctgtttagca acaagtaacg caatgatttt gaagtgataa ggagatgtgg
    40201 ctgaaaaatg ccgcatctcc ttttttattc ctaaatatga atacgcatta ttttcaatct
    40261 ttttgtagaa aaagggtaca atagaaaaag agaaaggcgg gagaatatgg ttatcaattt
    40321 cattatttta tttttgatga ttgcaattac ttcatttttt gtagcaagtg aatttgcttt
    40381 agtgaaaatc agacgatcaa gattggaaca actagaaaag gaaaatgtaa agaacgccaa
    40441 gctagctctt catgttacac accatttaga taactattta tcagctagtc aattaggaat
    40501 tacgctaacc ggtttgatca tcgggtgggt cggtgaagga tctgtcgcgg ctttactaga
    40561 accagtgatc ggtaaactac cttttagttc ggctattagc agtacaatct ctgtggcgct
    40621 gggatttatt ttagttacgt atatcgatgt ggttattggc gaactgcttc ctaagagcta
    40681 tagtatcgtc aatacggaga aggtcgtctt gttcgtagtt aaaccacttc attattttta
    40741 taaagtaatg tttcctttta tttgggtatt gaatcattca gctgcagggc tagggaagct
    40801 attaggtgtg cgtctcgtat ctgaaggaga agagacattg tctcaagaag agctgacatt
    40861 agtcgcattg aattcttatg aaaaaggaga gttgaccaaa gaagaatatt attatttgga
    40921 aaacgtgttt gaatttgatg ataccttggc aaaagatata caagtagacc gtacgtccat
    40981 gcaagtattc gaagcttcag aaaccgtcaa agaagcgata aaagcttcga ttgaacaagg
    41041 acatactcgc tatccggtta tcttagaatc aaaagacaat gttttaggtt atgtcacctt
    41101 gccagatttg atcaagcaat cttataaaga tgaccagctg acagtagaac agttgattga
    41161 agagccgatc gtaacaactg aaacgatccc tataaaaaaa ttattgacga tcatgcgaaa
    41221 aaagggtaag catattgcta tcttaaaaga tgaatacgga gggacaagcg gcttagtgac
    41281 gattgaagat attttagaag aaatcgtcgg ggaaatccga gatgagacag atttggacga
    41341 agcattgata gcggaacagt cagatggctc ttatatcatc tccggcaaac taacattaga
    41401 tgatttccag cgttattttc atgtagagat tccagagttt gaagaaacga attttactac
    41461 acttgcgggc tttgcttcta gccgatataa agaaataaaa gcaggaacaa tcattgagat
    41521 tgcatcattc cgtttcacgg tattagaata ccagcatgca catatcgatt atttcaaagt
    41581 agagtccacg gaaagaaaaa cagaataaaa gaaaaaaggc tgtgacattt tttgtcacag
    41641 cctttttgtt atctaaaaaa cctgttttcg gtaaacgaag aagatatttt caataaaagt
    41701 tttcattttt agccgcaacg atttctttta atcaaggcta tcttgcttct gcatgttcta
    41761 atgtgaaatg ttcctcttca cccatgtgtt tcaattgttt catcgaacgc atataagagt
    41821 aaagcaacac ggcaacagaa ccgatccaag ctaaaggaga agctgctgct gctcctgggt
    41881 atccccacat tcctgtaaga acgatagctg caaaagaacg cataagcaat tccatcatac
    41941 cggcgatcgt agggatcttg ctttgtccaa gcccttgaag tgtgtagcgt aaaatgaaca
    42001 ggactgctaa gatccagtaa agagacccat tgatattgaa gtaggtttgt gctaattcaa
    42061 atacacgtgt ttctgatggg ttaacaaaca attgaacaaa gaaatgaccg aagaaaatga
    42121 cgatagcacc tgctaagaga ctaaatccga cactcatcag taaagtttga cgtacccctt
    42181 gcaagattcg gccatattct ttcgcaccat agttttgtgc agtgaaagtt gccattgcaa
    42241 taccaaatga catcatcggc tgatttgcaa attggtcgat acgaccagca gcggcttgag
    42301 cagctacgac atctgtccct agactattta gtgctgcttg taacacgata gcaccaacag
    42361 cgatgatcga agattgaaac gccattggca aagcagcatt taggtgtaca gcaaactctt
    42421 ctttttcaaa tttgaaatct tttttgcgta actgtaacaa aggcattttt ttcttgatat
    42481 acactagaca cagtacgctg gaagaaactt gtgcgattac tgtagcaata gctgcacctt
    42541 ccacacccat gccaaaatag ataataaaga tcaaatcaag tacgacgttg atgatgaccg
    42601 caatgatcag aaagaatagt ggtgtgcggc tgtcaccaag tgcgcgaatg acattagaaa
    42661 gcaaattgaa actgactgat gcaaagatcc ctaataaaat aattgaaata aatgtttgtg
    42721 cttgatcaag tatttcagga ggagtttgca tcaattgaag catcggacga ataaacagga
    42781 gacttaggac agttaaaatc acagtgacga caagactaat gacaacacta gcagcaaaac
    42841 ttttcttcag accccgataa tcttttgcac cgtaacgttg tgcggtaata atggctaaac
    42901 cagctgttaa accttgggca aagccaatga tcaaaaatgt caggcttcca gttgcaccaa
    42961 ccgctgccag cgcattttta cctagcgtct gacccacgat gatcatatcg atcatattgt
    43021 agaactgctg aaaaacattt ccgatcagta aaggaacagt gaacatcaaa atcagtttag
    43081 cggggcttcc ttttgttaaa tcgcgcatct tttaaactcc ttatatcttt aaaatttagt
    43141 tagtagtata tagcgtttat cctgagaata ccaattagaa atcaatgata atttatgaaa
    43201 ctccataaaa agaagccaag aattgagtca aagaatcaac aacttggcct ggtctgtaaa
    43261 ataagggaca tccacgttta tctatcacct taatattgtc taatctcact tggtaaaagg
    43321 atcgtttctc tcgtaccgtc atcataggcg tatacttgag caggaaattg gttcgaatag
    43381 gcaaacggca aatcaatatc catttccaca ttgcttcctg cttgagaaaa atgcatcgtc
    43441 acttttccac gattttcgat caaatcaaag gttaatatat tttctaatgg aatgactccc
    43501 ttaagatcta ggtcaataat aaaccatata ctgtcaatca attcatctgg caaacttgat
    43561 acgacaccaa ctgaagcgta acggttgcgg ctgctatcaa aactttcaaa catctgttta
    43621 acctccttcg aatcaacgaa tgttttattt ttataattaa tcctaggata aactagaaat
    43681 acttacttca acgagggttc attctaataa agaatcagga aaaatgcaac aataattgaa
    43741 tacttttttt caaagtataa atttttataa aggtacgata aaagtatgcg aggaaaagct
    43801 tggtaaatta ggatttttcg tttgtaaagc catttacaaa agtggaagcc ccatatgaaa
    43861 ataaacgatt agaaataaaa aaggcgtgtt aaaagctgct gacccgccgc ttctaacacc
    43921 acctgattgt ttcagtatta ttcttctgtt gaaccgatca cttctggttc gccaccttca
    43981 cctgtgcttt cttcttcttc actttgagcg actacagcag cgatttgctc ttcactgtcg
    44041 gtcacgaatt tgtaatcgtc gttttttgga agatcggcta ctgtaataga atcacctaat
    44101 tccaagtttg tcacgtcaac ttcaattcgt tcaggcaatt tgtctggtgt agcagatacg
    44161 actactgtat acaagttttg agctaacact ccaccagatt tagcacccac tgattcgcca
    44221 acaagaacga cttctgtttc gacttctgtt tcttccgaca tgtttacaga tagaaattct
    44281 gcatgttcga aatgattggt aaaagtgttc gtttggatct tgtgaaccaa tgtattgact
    44341 ttttgtccat cgatgtccat cgtgatgact gtgttcgctc catgttcacg caacaatttg
    44401 cttagtgtca aaccatcgaa tgagataggg gtactttcta ctttataccc ataaacaaca
    44461 gctggaactt tcccttcgtg acgaagtttg tttcttaatg aacggggacg tacttctctt
    44521 ttacttactt ctaatgatac tgacataacc aaaattcctc cttaaaatgt gactgtctgc
    44581 tttggtaacg gacagatttt atttaagtga attctaaagg ctaatctcgt gcacatcatc
    44641 gaaggcaggg cgcttaaaaa agcgcaaaag gagagatgcg cgcatctccc cttagaaagt
    44701 caggattcaa cttttctcca ccaggtcatt acgctgtgta tgagaaatcc aattcacttt
    44761 actatttaac cgtaacgtga actgttaatt aagtcaagga ataactatga tgatgtattt
    44821 gcaagcaaga atattcaagg tataattaat tagtagtaat aagaaaggaa acagcatgcg
    44881 tttagataaa ttaatagaag aaaaattaga aacgacacgc aaggaaatga agcggctgtt
    44941 tgctttgaag caagtgatga tagatggcac agtcgaattt cgacaaaatc gaaatgtaga
    45001 tagtttactg catcaaatcg aagtagcagg tgaacactta gaaacaaatg agtcctatta
    45061 tcttctcaat aaacctgaaa aagctgttac agctgtatca gatccagaaa agacgacagt
    45121 aatcgatctg attgcaagtg ccgatcgttt ccaaccatta tatccagtgg gaagactaga
    45181 tagagataca acaggactat tgctgatcac ttcgaatgga caattgggat atgaaatgct
    45241 acttccagaa aaaaaggttt caaagactta tcaagtagtt gtgaacgaaa gggtgacacc
    45301 ggcagacgta acagcatttg aaaatggaat cgtattcgct gatgggtatc aatgtaagcc
    45361 ggctgattta aaaatactca aagcagacaa gcaccaaagt acggttttat tgacgatcca
    45421 agaaggaaaa ttccatcaag tcaaaaagat gttcttagcc caaggaaaga aagtgaccgc
    45481 attgaaacgg ctaagcatgg gaccattgga attagatgaa cagctaggag taggcgagta
    45541 tcgatcgttg acattagcag agatgaaaaa gttaagtatt tattttaaat aggaggtcaa
    45601 aaagatgaaa tataaaactt tattgtttga tgtagatgat acattgttag attttcaatt
    45661 aactgaagcc aatgctttaa gtgctttgtt tgcagaacaa ggaatcacat tgacaccaga
    45721 aatcgaaacc agctataagg ctttgaatca tcatttatgg caggagtttg aaaaaggcag
    45781 actatcacga gaagaaataa taggcagtcg ttttgggtta cttttccagc aatttgggaa
    45841 aacagtagat agtgatcaaa tggaaaaacg ctaccgccac tacttgaacc aaggtcatga
    45901 tcttgttcca ggaagtctag aaatcctgaa aaaggtccat ccgcatgctg atctttatat
    45961 tgtgacgaac ggtgtgtcgc acacgcaaca tcggcgatta acagatgcag gaatgcttga
    46021 ttacttcaag gatatctttg tctcagaagc tgtcggagcg caaaagccga tgaaagaatt
    46081 ttttgatcat gtcttttccc acattcctaa ttttgataaa aagcaaactg tgatcattgg
    46141 tgattctttg acttctgata tcaaaggagg aaatatggca gggatcgata cgatttggtt
    46201 caatagaaat aaattaccag aaatcgaaga aatccagcct acttatcgaa tcgattcatt
    46261 agaggaactt tattcattat tggaaattga aaaggattaa ttttcagaaa aatatagcat
    46321 tttcgtgttt tttcgctata atagattaga atgaaaaaac ggatacgaaa gaggttttag
    46381 tcaatgacaa aaagcaagcc aattatcatt ggtgtgacgg gtggatccgg aagcggaaag
    46441 acaagtgtca gccgtgcgat tttcaacaac ttcccggacc attccatcat gatgcttgaa
    46501 caagattctt attacaagga tcagagccat ttgagttttg aagaacgatt gaacacaaat
    46561 tacgatcacc catttgcctt tgataatgat ctgctgattc agcatgtagg cgacttactg
    46621 aactataaag ccattgaaaa acccgtgtat gactatgtag cgcatacaag aagtcaggca
    46681 acgatcattc aggaaccaaa agaagtgatc attctagaag gaatcctgat tttagaagac
    46741 gaacgtctgc gtgacttgat ggatatcaaa gtatacgtag atacagatga tgacattcgt
    46801 atcattcgtc gtatcaaacg ggatatggaa gaacggggac gcacattgga ctccgtgatc
    46861 gaacaatatt tgacagttgt taaaccgatg tatcatcaat ttatcgaacc aacgaagcgc
    46921 tatgcggata tcattgttcc agaaggcgga gaaaaccacg ttgctattga tttgattaca
    46981 acaaaagtag ctagtttctt aaaccataaa tagtgcgcag cgaaacagtt gaacggactg
    47041 cgaccgtttg aaaagcaagg attctgcttt ttgaacagtc accagccaac tgttttttta
    47101 tttataacaa gaggttgtga aaacagcatc ttgacctaag tattaaaaag aagaaacgga
    47161 aaatagctgt ttatatttta ccgttttttg agctaatacc gaaggtcagc tgattgagca
    47221 ccgtgaagta gaggttgtaa aaaagctgtc tttgcatcaa gcgataagag catccaaata
    47281 aaacagctcc tcacgttttt cgttgggttg ggcttattat cgtagatgca agcttttgaa
    47341 caccgtttaa cgagaggttg taacagaagt ggtcagatgc aataaataag aagaaactta
    47401 cgaaaattat ttctcgaatt tgtgtgagat ttcgacttat ttttttgaag aagctctttt
    47461 gtcgcacccc
//
LOCUS       DAFGZE010000017        46071 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.017, whole genome shotgun sequence.
ACCESSION   DAFGZE010000017 DAFGZE010000000
VERSION     DAFGZE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.017"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            41..922
                     /locus_tag="LTU54_000984"
     CDS             41..922
                     /locus_tag="LTU54_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295715.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            935..2545
                     /locus_tag="LTU54_000985"
     CDS             935..2545
                     /locus_tag="LTU54_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HBL8295716.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2622..3281)
                     /locus_tag="LTU54_000986"
     CDS             complement(2622..3281)
                     /locus_tag="LTU54_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL8295717.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3564..4229)
                     /locus_tag="LTU54_000987"
     CDS             complement(3564..4229)
                     /locus_tag="LTU54_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HBL8295718.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LTU54_000988"
     CDS             complement(4376..4981)
                     /gene="dhaL"
                     /locus_tag="LTU54_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HBL8295719.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LTU54_000989"
     CDS             complement(5009..6019)
                     /gene="dhaK"
                     /locus_tag="LTU54_000989"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBL8295720.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LTU54_000990"
     CDS             complement(5994..6440)
                     /gene="dhaM"
                     /locus_tag="LTU54_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HBL8295721.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            6543..6863
                     /locus_tag="LTU54_000991"
     CDS             6543..6863
                     /locus_tag="LTU54_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBL8295722.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(6975..7844)
                     /locus_tag="LTU54_000992"
     CDS             complement(6975..7844)
                     /locus_tag="LTU54_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8295723.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(7940..9244)
                     /locus_tag="LTU54_000993"
     CDS             complement(7940..9244)
                     /locus_tag="LTU54_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL8295724.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIANAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(9241..9750)
                     /locus_tag="LTU54_000994"
     CDS             complement(9241..9750)
                     /locus_tag="LTU54_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295725.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(9925..12060)
                     /locus_tag="LTU54_000995"
     CDS             complement(9925..12060)
                     /locus_tag="LTU54_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295726.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(12143..13798)
                     /locus_tag="LTU54_000996"
     CDS             complement(12143..13798)
                     /locus_tag="LTU54_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL8295727.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(13800..14114)
                     /locus_tag="LTU54_000997"
     CDS             complement(13800..14114)
                     /locus_tag="LTU54_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL8295728.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            14284..14544
                     /locus_tag="LTU54_000998"
     CDS             14284..14544
                     /locus_tag="LTU54_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBL8295729.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            14558..14872
                     /locus_tag="LTU54_000999"
     CDS             14558..14872
                     /locus_tag="LTU54_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBL8295730.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(15602..16156)
                     /locus_tag="LTU54_001000"
     CDS             complement(15602..16156)
                     /locus_tag="LTU54_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBL8295731.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(16282..18211)
                     /locus_tag="LTU54_001001"
                     /pseudo
     CDS             complement(16282..18211)
                     /locus_tag="LTU54_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(18517..19233)
                     /locus_tag="LTU54_001002"
     CDS             complement(18517..19233)
                     /locus_tag="LTU54_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HBL8295732.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="LTU54_001003"
     CDS             complement(19234..21057)
                     /gene="glpO"
                     /locus_tag="LTU54_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HBL8295733.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="LTU54_001004"
     CDS             complement(21071..22567)
                     /gene="glpK"
                     /locus_tag="LTU54_001004"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBL8295734.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(22703..24163)
                     /locus_tag="LTU54_001005"
     CDS             complement(22703..24163)
                     /locus_tag="LTU54_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295735.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(24153..25448)
                     /locus_tag="LTU54_001006"
     CDS             complement(24153..25448)
                     /locus_tag="LTU54_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL8295736.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(25780..26457)
                     /locus_tag="LTU54_001007"
     CDS             complement(25780..26457)
                     /locus_tag="LTU54_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HBL8295737.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(26487..27578)
                     /locus_tag="LTU54_001008"
     CDS             complement(26487..27578)
                     /locus_tag="LTU54_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HBL8295738.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(27679..28113)
                     /locus_tag="LTU54_001009"
     CDS             complement(27679..28113)
                     /locus_tag="LTU54_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8295739.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="LTU54_001010"
     CDS             complement(28697..30154)
                     /gene="ascB"
                     /locus_tag="LTU54_001010"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL8295740.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(30151..32046)
                     /locus_tag="LTU54_001011"
     CDS             complement(30151..32046)
                     /locus_tag="LTU54_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8295741.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(32104..33879)
                     /locus_tag="LTU54_001012"
     CDS             complement(32104..33879)
                     /locus_tag="LTU54_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL8295742.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(33929..35425)
                     /locus_tag="LTU54_001013"
     CDS             complement(33929..35425)
                     /locus_tag="LTU54_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8295743.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(35619..37241)
                     /locus_tag="LTU54_001014"
     CDS             complement(35619..37241)
                     /locus_tag="LTU54_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL8295744.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(37569..38882)
                     /locus_tag="LTU54_001015"
     CDS             complement(37569..38882)
                     /locus_tag="LTU54_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL8295745.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(39127..39351)
                     /locus_tag="LTU54_001016"
     CDS             complement(39127..39351)
                     /locus_tag="LTU54_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295746.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(39371..39793)
                     /locus_tag="LTU54_001017"
     CDS             complement(39371..39793)
                     /locus_tag="LTU54_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HBL8295747.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(39935..40645)
                     /locus_tag="LTU54_001018"
     CDS             complement(39935..40645)
                     /locus_tag="LTU54_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295748.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(40663..41160)
                     /locus_tag="LTU54_001019"
     CDS             complement(40663..41160)
                     /locus_tag="LTU54_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295749.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(41197..41649)
                     /locus_tag="LTU54_001020"
     CDS             complement(41197..41649)
                     /locus_tag="LTU54_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8295750.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(41784..42389)
                     /locus_tag="LTU54_001021"
     CDS             complement(41784..42389)
                     /locus_tag="LTU54_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL8295751.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(42839..43459)
                     /locus_tag="LTU54_001022"
     CDS             complement(42839..43459)
                     /locus_tag="LTU54_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HBL8295752.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(43461..44330)
                     /locus_tag="LTU54_001023"
     CDS             complement(43461..44330)
                     /locus_tag="LTU54_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBL8295753.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(44353..44760)
                     /locus_tag="LTU54_001024"
     CDS             complement(44353..44760)
                     /locus_tag="LTU54_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL8295754.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(44779..46068)
                     /locus_tag="LTU54_001025"
     CDS             complement(44779..46068)
                     /locus_tag="LTU54_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8295755.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        12925 a         9837 c         7891 g        15418 t
ORIGIN      
        1 ttgtttagta tatattttta gaaaaaggag tggggttttt atggcagaaa tcgtcaaagt
       61 cacacatttg aaaaagagct ttggtcgttt cgaagcttta aaagatgtct ctttcaccgt
      121 acattcaggt gaagtcgttg gatttatcgg accaaatggt tctggaaaat ccacgacgat
      181 tcggacactt ttagggatta taaaaaaaga cggcggaacg gcaacgattt ttgggcaaga
      241 tgtatgggaa gatagtttat ctatccatga acgtctttct tatgttccag gcgatatctc
      301 tttgtgggga aatttgactg gaggagcgat catcgatctc ttcatgaagc ttcatggaaa
      361 agggagtgct caaaaaaaag atgaactgat tcaacgtttc tctcttgatc ctaagaaaaa
      421 agcaaaaaac tattcaaaag gaaatcgcca aaaagtgggc ttgattgctg ctttggctgt
      481 tgattcggat ctctatttat ttgatgagcc tacttctggt ttagatcctt tgatggaaac
      541 gattttccaa gaagaagttg aaaaattgaa agaacagggc aaatccatcc tactttcttc
      601 tcatattctc agtgaagtcg agcgactagc ggataaagtc gtgatcatac gcgaaggaac
      661 actcgatgaa gaaggaacac tcgatgaatt gcgtcatttg actcgttcga ccatcactat
      721 agaaacagaa gcagagaata gtcacttagc acaattgaac ggtgtccatg actttgtgca
      781 aaaaggtcac ctcgctcaat ttgctgctga taacgaagct ttcgatgtga ttttagctga
      841 agcgtccaaa ttaggtgtga aaaaatttga atctattcca ccgactcttg aggatttatt
      901 catgagacat tatgaaggat gaggaggaag cgaaatgact gataaatttt cacgctggtc
      961 cacccttttt tctcaaaacc ttagccgtga ctggaaaaag atcctgatat ggctagtcgg
     1021 tgtcactgct ttttctggtg gttttgtccc tgcgtttgaa gaattgactt ccggacaagg
     1081 attagctgga ttatacgaaa ccttgcaaaa cccggcaatg attgcaatgg tcggaccaac
     1141 tccagcttcg aatgctgctg agtatacttt aggagcgatg tatgcaaatg aaatgttgct
     1201 tttttgcagt ttgtttgctg ctgtgatctc tgttttacac gtggtgagcc aaacgagaaa
     1261 aaaagaagaa cttggtctga ttgaacttat tcgttccttt cgagtcggac gtcaagcaaa
     1321 ctctttagct gtcatcgctg aaagtattct gatcaatttg cttttagcaa tatcgatcag
     1381 ctgtacgatg atttttttcc aagcggattc gatcactgca gaaggtgctt ggctttttgg
     1441 gctatctgta ggtagtatgg ggattttggg gagtgccgtt gcactgtttg ctgcacaact
     1501 gatgccaagt tcagccagtt cgaatggtct gtctttagcg tttttaggtg gactttacct
     1561 tctacgagca ataacagatg tagctgattc ctccctttcg atgtggaatc ctttaggctg
     1621 gtcttatttg acctatccat ttacaactaa caactggatt cctttgcttt atttgttttt
     1681 gttcagtcta atcttgataa tcagttcatt tgttttagag gaacatcgag atttgaacac
     1741 cggctatatc cccgaacgtg aaggaaaacc gtatgctaaa cgttctcttt tatccttgcg
     1801 tggtttatta ttctatctaa ataaagggat gacaatcagt tggcttatca cttttggttt
     1861 gatgggggcg gcatacggct ctatctacgg gaatatgcaa cagtttttag aaagtaacga
     1921 cttaatgaaa cagatgttta tccaatctgg cgtctcaatt gaaaaaaact ttacaagcac
     1981 gatcatggta gtcttgattt gtctagctgc tattttacca attgctagca tcaatcgttt
     2041 atttacagaa gaaacccacg gacgtatgaa tcagctgttc gcaacaaaaa tttctaggaa
     2101 ccaactttat tggaccaata tgttactagc tattctattt gctatattgg caatcctgat
     2161 tagttctggt gcactagggc tttctgcttt ggcttcgatg aaggatgcga aagacatgga
     2221 tctattgatg tttcttaaag ccgggttcaa tttcctgcct gccgttttag tgtttatcgg
     2281 aattagtggc tttctattcg gatggcttcc tcgaatcggt aaacttgctt atatctactt
     2341 agcttactcc tttgcactca attactttgg cggaattttg gatttaccag aatggttttc
     2401 caaaacagct gtccctagct gggttccacg gatgccaaac gattcttttg atctttccgt
     2461 gttccttacg ttatcatgta tcagccttgc actgttcgtc cttggttcta ttggttatag
     2521 gcgaagagac ttactagaag gataataaaa aagcatgtac aatttcatgc tagcctccaa
     2581 atctgggtca aaaatccaac atttggaggt cattttttta tttacaagtc ttctaactgc
     2641 tctttatcaa tgatccagac ttctcgattc tttcttttta cataaccatt ctcctctaat
     2701 aatttaaatt ttctagataa agtttcgggg gtcgtaccga taaatgcagc aagttctttc
     2761 attttcattg gcaggaaaaa gtgatccttc cctgtcgctt tagctaagtc aagcagatag
     2821 acagcgactc gttcttctac tttttccatc atcaaaaaag ttgtctgctg ctgtactttt
     2881 gttaatttct caacgttgat tgctaaaagt ttcagactaa gggcgggata atgcagtaag
     2941 agttccgaga aatctttttg tctcaatatg caaatttccg ttttttctag tgcttcgcca
     3001 aataaagact catttggaac gcctagcaac tggctctctc cctcatatcc gcccggctct
     3061 attactcgta aaagctgttc ctttccgcta gcagataatt gatacacttt catactgccg
     3121 gaggccacga tcactaattg aggttctcca ttcggcgcaa gaatctgttc tcctttttcc
     3181 acatagtgat gtttgacaag tgcgtgtact ttttcttgat cttgctgtac taagtgattg
     3241 aacaaaggaa caaatgaaac acataaatga tcatgactca taatctctcc tccttctaaa
     3301 cagctatttc taataaagaa acataacttc ccttattcca actgataatt ttcttttatc
     3361 atagcataaa cgtcaagcga accagatacc agcaaataga gtggatttct ttttgtccct
     3421 tgctttattt tccttctttt gagaccttaa gtaattgatt cctatcaatt ttttaaatat
     3481 ttactttttg aataatcaaa aacaaacctt cactcgcaag ccgagagtaa ggtttgtttt
     3541 tcgcattgct tatttcgtgt tgtctattga aatttttccg cacctagctt ttgatatagt
     3601 ggtgctagtt cttgtaaggt ttcagcggtt atttgctcaa cgtgttcgat ttcttgcgga
     3661 tagaagaata gtttacccat ttgaaaggtt cggtctctca tcgctttttt caactcttct
     3721 tgtttctttg tatcggacaa atagtcagac aaagctaagt ggtcgtcaag ttcatgttct
     3781 ggttgcggcc atatgtaata atccttgata tcgactgttt tgctccactc tttcagtatc
     3841 gcaagcaact ggttatacgc tgcaatgcta ccatatcgtt cttcgctttt ataatgttgg
     3901 tacatcaaat agatttggta ttgtttttga ttcaacaaaa ctgctagaca ggcattctcg
     3961 tcttgacgag attcgcttcg gtacgcgctc caaaaatgac tgcgtaaatt ccagccattc
     4021 gtccagcttt cgattttagg tttgctcatg ccgtacatat caggcaaaag tgcagctgtc
     4081 tggagctgaa tctctttcca tttttgccag tgttctttat attctttctt gatttgttca
     4141 atcttatctg ttggatggct ctctttgagc tgtttgaatg cgtagattgg tcgatcaaac
     4201 aactgataat ctgattttcc aagttccatt gtttcgcctc tttccgttat cttttgtatt
     4261 ctacctgaat tttgcgatat ggacaaatag aggttgtgaa aaagctgttc tgcatcaagt
     4321 cataagaaca gccaaacaaa aaactgcgat gagcatcacc gcagttttat tgaacttaag
     4381 gttgattgat cacatctaaa agcgttgaaa ataaaatggc actagaagca gctcctgggt
     4441 cgatatgtcc gattgcacgc tctcctagat aggaagctcg tcctttttta gctaccaaga
     4501 ctttggtgtg ttcttttgcc tggtcaatga tttcttttgt taaattccct gccttcaatg
     4561 cctctgtgac tggcagccaa acatctaaca tcgttttatc gtcagcttct gcctttcctc
     4621 ggctttgaat acccgctgtt ccctgctcga tcacttcgcc taatttttct tgagaatcaa
     4681 tagaatcgac ctcttttgtc gctttcatca tgttcatgaa tgcagatccg tataacggac
     4741 cagaagcccc accgactttc gatatcatcg ccatagagaa tactttgaat gtatcgctga
     4801 ttgtttcagg atgatcggct tgaaaagctt cttcataagc tgaaacccct cttcccatgt
     4861 tatttccatg gtctccatcg ccaatcgggg tatctagatc actgagatat tgtttgttct
     4921 gattgattgc ctctgcaaat tgcgtgagcc attcttggat ttcttgaacg gttagtttca
     4981 tattatcacc cttttatctt gagatttttt accatgaaat cgtctgtaca ttgctttcta
     5041 aagaatcttt ccattgtgtc tccgtcaagt cgatcagtgt tagagaaaga ccttgcatat
     5101 ctagtgaggt cataaaatta ccgacttttc ggaaaactac attgacgcct ctttcttcca
     5161 acagagctaa aacatcattc atgaacacga actgttccat caaaggcgtc ccgcccattc
     5221 cgttgactag tacacctgct tcttttggta aattcgagta atcatctaaa atcttagtaa
     5281 caagttcctt tgccagtact ttagaaggct gcatcttttc tcgacgatag cctggctcac
     5341 cgtggatacc aacaccatac tcgatctcat cttctcctag ttcgaatcct ggtttcccta
     5401 cttctggtac agttgctgct cgaagcgcta gaccgatcgt cttagttgcc tgtacgacct
     5461 tttctcccag ttctttcaac tcagctaggg aagcaccgtt tcttgctgca tcgcccacga
     5521 ttttgtgtac aaggacagta cctgcaactc cgcgttttcc agcagtatac gtactatttt
     5581 caacggcgat atcatcatct acgatcacac tttctacttc aatatcgtcc atagcagcta
     5641 attcctttgc catatcgaag ttcaatgcat cccctgtata attcttaacg atcagcaaaa
     5701 ctccttttcc actatcggct tccttaatag ccgtctgaat ctgatctgga gttggagaag
     5761 taaacacatc accaagcacc gctgcactta acatcccttc ccctacaaac ccggcatggg
     5821 atggttcatg tccgcttcct ccaccagaaa ccaaaccaac ttgttccgct ttctgattct
     5881 tggctacaac acgggaagat tctacacgat gaacaagttc tggatagctt tttactaatc
     5941 cttccagcat ttcttcgaca atattgcttg gatcattcat gatttttttc aatttattcc
     6001 catcctttct ttccggcatt ttgtgcttct gttaagattt gctgagtatc atctgttata
     6061 ccggcagtga tggctgctgc aaaagctcct tccactaaag gcgcatccac cagatgataa
     6121 cgttgctgct gctcttcttc caacatgtct tttgccagtt ccgagttcaa taccgcgcta
     6181 ccgatatccg caaaaacaaa ataactagct gcatcgcttg attcattgac tgcttctacg
     6241 attttcatcg ggtctgatcc gatctcgcca tttattccgc cgagagaaaa aatttgcacc
     6301 tcttcggatt tttgcatctg ctcaatcatt tctttaatac catctgttac tttttgacta
     6361 tgagaaataa gcaaaatact ttttttcaca agaatcttcc tttcttttac atagttgcta
     6421 acgattggtt tcgctttcat taatgaacaa ttcaagtata taataaattt ctaattttgg
     6481 gaaaatatgt gtagtgcttc ttgaagtttg ttatacttta attaaaagag aggagtgatc
     6541 gaatgattac aacaacaacg aacagtgtag agggaaaagc cgtagtagct tataaaggga
     6601 tcgtttttgg tgaggtcatt actgggatcc atgcatttaa agacttggag gcggggttgc
     6661 gaaatatctt tggcggccgc tcaaaaagct atgaaaacga actaatgggt gcaagagaag
     6721 aagcacttgc cgaaatggcc gaacgtgcaa aagcattagg agctgatgcg atcattggcg
     6781 taaaaatgga ctatgaagta ttaggcagcg acaatggcat gctgatggtc acatgtagtg
     6841 gaacagctgt ctccacgaga taagtagggg acatctgttt taataactaa agagagatgg
     6901 gacatgtagt ggcactcaag aattagattt ttcagtctaa cttcgaggta ctctaccttc
     6961 ccatcctctc ttttttattt ctttttgcgg atgattccgt aagctgctgc gactaacaca
     7021 cccgcccctg ctaaggaata actgacagaa gctgtttttt catttgtttt tggcaaagtc
     7081 tgacttgttg atttggatgg ctctgtcgtt tgctgaactg tttctgtcgt gcttccagag
     7141 gtcactgatt tttctcctgc cgaatgcaaa atatctttta ttcgttggaa actagctgct
     7201 aagtcagctt tttttgcttc aaaatcatta gcaactgttt gtgcttcgct gatctttccg
     7261 tggattgctt gactggccgc tgtgatatcc gcttgagttt gacttagctt tccagaagct
     7321 gtttgagcaa cctgatttaa ttgctgattg aattcttttg cttgttggat cttcccgtca
     7381 agatctgttt ggacagcttc cgcttttgct ttcgcttcgg caagcttttt ctcaagttgt
     7441 gcacggtcat ttcccacttt atttttaaga tcagctaaat cttgatcgat tgctgcttgg
     7501 tcttctttca atttgttttt tgcagcttct aatgcttctt cgagttcttt tcttttattt
     7561 tccaagtctt gcaaatgatc gatttggtca gaagaggtag aactatttat tgatgtatct
     7621 tctgaagatg aaatcgtttc tcctgaatta tctgtcggta cattggaagt catttctgtt
     7681 tccgaggcac cgttttcagc accagaagag cttatggttg ttgaaggttc tgtttgagca
     7741 ggttcagaag tttccgcaaa agctgtctgt cccaataaga aaatactgga aacagcaatt
     7801 ggccagatta acggcttcat tctttttttc atcacttttc ccatccttac tactttttaa
     7861 ctaatttctt attataagca aaaatgtttg gaagaaccat aaacacaaca ggctgtttgt
     7921 ctaaaaaacg gtgcatcttt tatttttttg tacgttccgg gcttttccat gtcccgcttc
     7981 cataacctaa aatcgttttg actgctggaa tgaaagtcgt cacgatcgtt atcgcgttcg
     8041 ctatccagaa caaaagcata tatagtggtg cgaaaagaag gtatttcaat tttcttcgtc
     8101 gatcatctac aatcagcgaa gctagcaact ggaaaaatcc tgcgaccatt tcaaagcaga
     8161 tgaacacaaa actgaatgta aaagtaatat aaatctgttc gtagtttcct tgatatacat
     8221 aatgcccgat taatccagca aaaagaacta cggaaataca aaagaatatt gaccagacaa
     8281 tactcaatgt ttgatcaatg aagatgatcg ttcttccaat atgtttaaaa ggatggagca
     8341 tcactttttt gaaattcgtc agccagactt ctgttccacc ttttgcccaa cgttttctgt
     8401 gtcgatatag catcttcaac gtcacaggta cttccatgaa aaagataatc tcaggagcaa
     8461 aaacagataa ccagtcattt aactgatgat cccaagcaat actgatgtct tctgtcgctc
     8521 gatcttgtct gaatccgccc acatcgatca aggcttcttt tcgatacatc gtatttgctc
     8581 cactgtacgc ataaagacca ccgaaaacag ctgactgtgt tcgtttaatg atgcccacga
     8641 tacttgagaa ttcaaccgtt tgagattttg caatcaattt ggtacgattt tggacatcca
     8701 tatttgcagt gaccgcagca atatgtcttg caccagggcg aatgaaataa ttcacatacc
     8761 taatcagtgc atctggctcc ggtaccgtgt ctgcatcatt gcttaaaatc aatttccctt
     8821 ttgcaaaggc aagtccgata ttaaacgcgt gggcttttcc tttatttttt tcgatccgaa
     8881 ccacacgaag attagcgtat tttttcatca gtctagctaa tatttctggt gtttggtccg
     8941 ttgaaccatc gtctgtcact aatacctcat agttgtgata attcaatttc gtcatcaaat
     9001 actcgattgt atattcgatc acaatttctt cattatgggc tggcaccatg atcgtgatga
     9061 acggttcgac actcaaggga acatctaccc attctttttg cttatgtttg tataaaaaca
     9121 cataacacca gacaccaata aaccatacaa atccgcctaa aatcggataa gaaacgagaa
     9181 aaaaatacat aatagacaaa caagtatcta agaataaatt acctgtatgg gtgattgaga
     9241 tcatttcagt tccaccccat tttctttata caaatctgca atgaatgtcg tatctagatt
     9301 tttctcttcg gagacagaat aaaaacggac cgtttctctc tcttcttttg ttccaaaacg
     9361 ttccgtatac acttcttcaa gtaattcttg tctttttttc aatttttcat cttcatattc
     9421 aaaagattta tttacttttt tgtatagatt tcgattgttc cacctagtca atacaaatga
     9481 ggtaatcagg aaactgaaaa aaatcaatac aaaaaagccg attaagaaat acagcatttt
     9541 cttttctgat aaaaaagcgt acaaggaaaa atccttggct ctttctggtc tcgttaatgg
     9601 gaaaaagacc catataaaag gaagaacaac gatcatccat cctaaaagag ccactaacgt
     9661 ttgccaaatc tttaaccagt aattaccttt tgaaaaatac ttatctttca ccaaaattga
     9721 tgcaacttct tctcttgtta cttttttcat atcctcacct cacgtttttt tgttacgacg
     9781 taggagttta ccatacattt catttctttt cacataaagt gttcctaaat ttttcctaag
     9841 aaaaagaaca aaacatttct catcagcaaa tgaaaaatat tttgttcttt ttatcagttg
     9901 ttctttttat tttttatctc ttaattatgt tgctctttcc aatacgacaa tgcttttcat
     9961 tcctctagat atcgccgtgt acatttgtct gcgtcttctt tgctcatctc catcgttttc
    10021 aaatggatat aagattacat gatcaaattc cagtcctttt gctagactca aaggaatgat
    10081 ttggcagttt tctaatccat atgaattttt tttaatcgat tcttgtagcg tttgggtttc
    10141 ctcttctgtt tgggtgataa ctgccgcttt ttctcctttt agagaaacca aaatctcagc
    10201 tagtattttc aacaattctt cagaggatcg aaaagagcgg tattcgggtt cttttccttg
    10261 cggtcggatc gatacgatgt cgatcttctc ttgatccaca gcataggttt tgaaaagctc
    10321 tgtgattgct ccgctagaac ggtaactatt tctaagatca taccgatgga tcggcagatg
    10381 gtagtcgtcg aaacaacgca taatatcagc aaaagtagtg gaagaattaa agattgcctg
    10441 attctcgtca cctactaaag taaagtgagt ttttggaaat aattcgatca atagacctaa
    10501 ttgtgccagg gtataatctt gtacttcatc aatcaataga tagcgaaatg ctggtacatt
    10561 taccttttca acaagtaaat gacgaatagc taatataatg actgcttcat caacagtacg
    10621 tgtgggttgc caagcataag ctgagccata tcgtttttca taaattcttt caaacaactg
    10681 atgctcatcc acccatgcca tttcttcgat ttgtctggtt atttttcgat attttttttg
    10741 taaaagtttc cttgcataag ctacaatact ttgttccgaa tcatcagaaa tcaatttacc
    10801 gaataattcc aactgcctat cttctgtcaa cgagagtacc tgattctgca cagcaggact
    10861 ttttgcttgt ttcaaaagat gattcttcca ttcgctcatc agtgcctttt tcgttgcttg
    10921 aagcctatcg atcaatttag gatgagcagg aactttctca tatatttttt cgatcgtttt
    10981 tttagaaagg actgtttttc catttttagt aagtccacgg ataaaattcg gatgatctaa
    11041 aaacaaagga tctgattgtt ttagattatc gataaacttt ttgctacgca aacgttctgt
    11101 ctgctccgat acattttctc ccgtgatacg tttgaaataa gcctcttcac cttctatttc
    11161 ctctgctgaa agctgtgaaa ccagctgtat gatggttaga ttcagtggat ttctttcacc
    11221 taatgatggt aggacattcg aaatatagtc gataaaccga tgattaggtg atagaatcag
    11281 caagtcatcg gatgtcatcg tatctcgata taaatagagc agatacgcaa tacgctgcat
    11341 gatagcagag gttttaccac ttcctgctac gccatttacc aagatatttt gacttttcac
    11401 atcccgaatg attgtgtttt gttccgattg gatcgtagca gtgatagcct tcatttcatt
    11461 ggtctcatct tggcctaaga cttctaataa tacgtcatct tgaatagcta cagacgtatc
    11521 aaaaaacttg accaaacgat ctctttccaa aagaaattgt cgtctttctt taataaccgt
    11581 atcgatttta ttttgacgaa ccgtataaga agatggacca atcatgttat tgtaaaaaag
    11641 ttcggcaatt ggtgaacgcc aatcataaac aagattttca tctgcatcat ctgtgaaatt
    11701 tgctgtaccg atataaaagg attcttttgg ctcattatcc agaaagtcta caacgacttt
    11761 tccaaagtag ggacttttca ataaacgttc gatctttgct agctgatttt ctgcgttttt
    11821 ctgtttgata ttcatctgat cgatttctct atttttcatt tcaatcatcg aaaaagtatc
    11881 caggttatct aagtaactgt caaaattcaa tcgaagctcg ccagccattt ctttcaacga
    11941 ctggtttcct tctatttttg ccgtatccat cgattcccgc agttcgtttt ttttatcaat
    12001 tagcttttga tacacgttct tcagatatgc ttcttctaac tgtttttgtg tgttgaccat
    12061 acacattctc ctcttttttt aaagtggtgg aaccgattag tttacctctt ttaagagtcg
    12121 aatgcaagct ttttaacgag tatcatttgt taggatatat cagttcttca ggtcggacag
    12181 tttgatataa agaaaatgca ccatcaaggt tttttacgtg gtagccagac tgcttcaaga
    12241 ttctttccgc tagatagcta cgtaatccgc tatgacagct gacgatatat tcttgttggc
    12301 tgtctaattc atttaatcgg tcccgaagtt cattcaaagg gatatgtttg gcttccttga
    12361 atttgccctt ttccaattct tctgctgttc gcacatctaa taaaattttt ccttttgcta
    12421 gttctgtcgg caattcatgc cattgtacgg tttcactaag cccttctgca atgttcattg
    12481 ctgcatatcc tagcatgttt actggatctt tggctgagcc aaatggcggt gcatacgtca
    12541 attctaattc cggtaaatcg aagatcgtca aatgcccttt gattgctgtt gctaaaatat
    12601 cgatccgttt atccacacct tttgccccaa caccttgtgc accataaatc tcgccagtct
    12661 ctggatggaa gatcaacttc agtaaaatat ctgttgcttc tggataatag cttgcatgat
    12721 cttttcctga tacatgaatg acttcaaaag acagttcgtt ttctttggct gttctttcac
    12781 ttaaaccggt ggatgcggca gtcattccaa acgttcgtat aattgctgtt ccaataccgc
    12841 cttgatttct tcgagaaatt ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg
    12901 ctggtgaagc aagagaaata agtgcatctt ttccagtgat ttgctgtttt acaacgatag
    12961 catcccccac cgcataaata tcaggaatat tcgtttgata acgttcgtct actaggatac
    13021 ccccgcgtat gcctaattcg attccagcgg tcactgctag ctggttatca ggacgaacac
    13081 caatggataa aataactaaa tcagcaggga tggtacgccc atcatccagt cgcaatcgtt
    13141 tcccgtggtc ttcaaatcca gcgacagcat gagaagtaat cacttctact ccttgatgag
    13201 acaattcttt ttctataaaa gctgccattt cttcatccag tgttggaaga atatgtggtg
    13261 cttgttcgat caccatgact tctaaacctc ttctttttag gttttctgcc atttccaatc
    13321 cgatgaatcc tgcaccaacg atcacggcac gctttggctg tttttctaat gcatgcatca
    13381 ctttatcgat atctggtaca tttctaatag aaaaaacatt gtcggcttct gttatccctg
    13441 gaatcgatgg aacaactggt tttgcaccag gagataaaac caatgcatcg tactgttcaa
    13501 tgtgtttttg accctcatgt acgacttcta ccgttttgtt ttcgggaaag atggctgtca
    13561 cttcatgatt tggacgcaca tctaaacaaa aacgagcgga taatgattct ggcgtttgga
    13621 caagaagatt ttcccgttca ctgatttctc ccgaaagata ataaggcaag ccgcaatttg
    13681 caaaagaaac atacggtcct ttttcaaaaa caacgatttc tgcatcttcc attaatcggc
    13741 gaagccgtgt agcagcagac attccacctg ctacacctcc gacaataacg attttcatct
    13801 tatttccctc ctactgttcg atcaaaccat tgattcatgc caccacgtac attcactaca
    13861 tcataaccag atttctttaa ttctttagct gcttgtttac tcctcatccc tgattgacaa
    13921 atcacataga cggtcttttc tttgtctcca tcatatctgt ttatactttg taatggaaca
    13981 tttttagctc cttttatatg cccgccccgg tattctgcag gtgttctaac atcaagtaac
    14041 gctggatttt ccaaaagctt ttcctttaat tctgggactg ttatcgattt tattgattga
    14101 aatagcaaat tcattttctg cacctcattt acatttttat aaatacctca tggggtatat
    14161 taaacaggag ctttattaat atgtcaacca gaatgactct tcattttttt cactactttt
    14221 tatatttgca taaaataccg ataaggggta ttatctaatt ataaaaatag aggagaatgt
    14281 gttatggaga ttgataataa aaatatcctg aaccgtttga aacgtacaga gggacaaatt
    14341 cgtggtatcc aaaaaatgat cgaagacgaa aaagaatgta tggatgtcat cactcaatta
    14401 agcgctgttc gttctagtat tgatcgcgta atgggcatga tcgttgcaga taatctgaaa
    14461 aattgctttg agaaacctga tagtaatcca gaagaacaag cggctaaact agaacaagcg
    14521 atcaagatga ttattaaaaa gtaaaggaat gatttcaatg aaagtcgttt tccatattga
    14581 tgaattagaa aaatggtcag aaacagggaa aaacgtaaaa aacctgataa aagcttctcc
    14641 agaaacgacg attgtcgtat ctgtaaatgg catcgcgatt accggttatc tagactctgc
    14701 caatgcagaa ttcttagatt tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg
    14761 tgctaatcat atttcagaaa gttctttacc tgaacaagta atcgttgtac cagcaggtgt
    14821 ccttgattta gttgaactgc aatcccaagg ttatgcttat atcaagcctt agtcagtaga
    14881 ggttgcgaca gaagtgatca gcttcaagaa atttcggctt atttttcgaa gaagctactt
    14941 ctggaaaacc gtgaatcaaa ggttgtgaaa acgctgtcct gcatcaagta ataagaacag
    15001 ccaaacaaaa atagcttctc atatttttcg tttggttggg cttattaccg cagatgcaag
    15061 cttttgaaca ccgtttaagg aagaggttgt gaaatcagcg gtttgaccta agtattagag
    15121 aaaaaaacgg gaaaatagct cctcatattt tgccgttttt tgagctaata ccgaaggtca
    15181 gctgattgaa caccgtttaa ggaagagatt gtgaaaaagt cgcttagttt cgagtattaa
    15241 gaaaaatttt gtgaaaatgg atttctacat ttttgcataa atttggctta ataccgagaa
    15301 actggctttt gaataccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat
    15361 tagagaaaca aacggaaaaa tagctgctca tattttgccg ttttttgagc taataccgaa
    15421 ggtcagctga ttgaacaccg tttaccaaga ggttgtgagc ctaccattta actccgaatc
    15481 acaagaaata atttcaaaaa caacttctcc tgtttttggt tcggttggct tattatcgag
    15541 aagtagctac tgggagtcta tttatcaaaa aaaggaatgc gacataggtc acaccccttt
    15601 tttattttct ttgaaacatc tttcggtact cgacagcata caagacaaaa gattgtaaaa
    15661 gaccgtaagt gatgacggca aaaatgactc tcacccacag cgttggtaat tctgggaaaa
    15721 gcagcaaaag gatcatgcca acatagatga cagcagtcgc tactttgcca taccacttcg
    15781 ctccacttaa tttgatattt ttagatagcc cgattaatcc agctacaaac ataaagcctt
    15841 cttttatcaa gaatagacca aacaacagcc aaagccacgg tcgataccat gccatagaca
    15901 aaatcagaaa aagttgtgtc aatttatctg caaacggatc aagcaccttc cctaagtctg
    15961 ttacctgatt atatctccga gcaatgaccc cgtctagata atcagacaaa cctgagacaa
    16021 caatgaccgc tcctgctata taaaatgact gtctcagcac aaaataaaga taaatcggca
    16081 gcaaaaaaat acggaatatc gataaaagat tcggaatcgt ttgtatttct ttacgccagt
    16141 ctgtgcttaa tttcatttta tcgccctacc tcctcagcac tcttttcttt ttattttagc
    16201 ttacacgggg aataaaacca atgatttcac tgtttttatt tttctttaca gacactgata
    16261 ttatgagcta atttgagata atcacttgct gttttcatcg tttactcttc tttcttcctg
    16321 tcgtttgcgc atttctttta ctagtacttt atgagctttt tttgatcctc gctctaggtc
    16381 acgctgccgt ttacgtgttt tataatcgat aaacagactg tctaaatcgt aacctgtcgg
    16441 gtacagatct tctgctgaac gcaccagacg aacacgctga cgaggcacgt ggattatttt
    16501 tttgtctaaa tatacatcaa tcgtatcttc acctatatct tcataaacta acgcttcttt
    16561 ttggtattca ttgacaaata tccgatcacc ttttgcaaag agctgacgtt cttttttatt
    16621 ctccttccta tttgtaactg ctgtcttagg aagaaattct tttttctgtg tccgataggc
    16681 tttttccgcc aagtaacgct ctgcttgatg gatcaatttc atcgacatat ccattttatg
    16741 agcaatccac aaagcttggc tttctcctgt ttcttccaac ccgcaactga tatttaggac
    16801 gaagtgcttc ccgatcgaaa gccatagcag ccggcacaaa atcttcatga tctccggcga
    16861 atttcttgat ttctccataa tgggtcgtgg caatgatcaa tgccccttgc tcatacatag
    16921 attccatgat tgcgatcgct agtgcagccc cttcatttgg ctctgttcca ctgccaatct
    16981 catctaaaag taccaacgta ttccttttta tctttttcaa aatagcagcg atgttttgca
    17041 tatgaccaga aaatgtactc aacgcatttt ctaaattttg gtggtcaccg acatcgacga
    17101 aaatctcatc aaagacagca agctctgtcc cttcttttgc cggaacttgc ataccgaaca
    17161 tcgccatcaa agtcaataaa ccgactgtct tcaacacgac tgttttgcct ccggcatttg
    17221 ctccggtgat aatcaagcct cgatagtctt taccgatttc aacatccaat ggtaccgcat
    17281 gctcaggtaa aaatggatgt ctgccttgct tgataatgat atgttccgat ttattgattc
    17341 taggtgtgct tccattgatt tctcggctaa attttgcccg agcaaagatg atatctaaca
    17401 ccgtgatcgt ttcgatcaat aaatcgatca cttcttcgtt ttctgcgata gcaccagtca
    17461 atgccgccag gacttgatat tcttcactga tttcttcagc ttttaacaac tgatagtgtt
    17521 cattcaattt agaaatcaca gttggttcaa taaaaaccgt cgttctttta tttgattcat
    17581 cgataatcgt tccatctatt ttatttttat agctggcttt gataggaatc gtatagtatt
    17641 cgcctttttg aacaatcatt gcttcttgga tcatttcttt gtttttcgga tgacgcaaga
    17701 atttcagtaa ttttgactgg atttcttttt cgattagttg taattgtttg cgtactttac
    17761 gcaaattacg tgacgcgtcg tcactgactt tattattttt tattttctgg tcaatcaact
    17821 cttctacatt tatcaaatca ggaagatgtt tgctataagc aaacaacaag ggtgtctggt
    17881 attgattctt atcaaaaaac tttgtgatca tccgactgct tctaagaaaa tccgcatatt
    17941 cgatcaaatc ggttggctgc agaatcaacc cttttttgac ttgttctgtc agtgcatcga
    18001 tcctcggcaa ccccataaat ggaacatgct gactgctttc taagatcaag cgtgcttctc
    18061 ttgtttcttc ttgacgatcc aagacagttt gtaggtttgt cgagaccgtc atatcgctga
    18121 ttcgtttttt gctatagttt ccaattgctc ttgcttggat ttcctttttg atccgatcaa
    18181 attgtaattg actaatcatt tcgttgtcca tattctctcc ttttcttgaa gagatacctg
    18241 tcccaaaata aataagggcc tagagataaa taaaaggata gaacacagct taaaaaagtc
    18301 atgtcctatc cttattagaa tacaaagata ttgttttcct gacaggtacc gtttcataag
    18361 cacaccaaat aaactttgtt tattggctat tcgacttact gattagatac cagctacact
    18421 cacaaaacat accccattct tctaggtctt atttattttc cacttaacta tagcaagaca
    18481 cattttggtt gtcaaacttt agtatgtttt atagatttac accattaatg aatagacacc
    18541 taccgcgata attgctccga tcattggacc tgcgattggt acccatgaat atgcccaatc
    18601 tgaagttcct ttattagcta ttggcaatac cgcatgtgct aatcgaggac caaagtcccg
    18661 agcagggttg attgcatatc ctgttggccc tcctaatgaa agaccgatgg ctaagatcaa
    18721 tccacctaca gcgaatacgt tcgttccagg tgcaaattcg ttttgtccaa aagctagcaa
    18781 cccaagaact aatacaaatg taccaattaa ttctgtgatc acatttgctg gataattccg
    18841 tatagcagga cctgttgcaa acgttcctaa aatcgcgctt tcatctttcg ttgcattcca
    18901 atgtggcaaa taagaaagcc aaacaagaat ggcccctgca aaagctccta aaacttgcgc
    18961 aatgatgaaa ggaacgacaa gattccaact aattgcccct gtcatcgcca tagctagaga
    19021 gactgcagga ttcaaatgag ctgggcccat gaatccagaa atatagacag ccattgttac
    19081 agcgagtccc caaccaaatg cgatgacaat ccagccagaa gcctgtgctt tacttttgtt
    19141 taaattcaca gccgcacaca caccatctcc aagcaaaact aggatcagtg tcccaataaa
    19201 ctcccccatg atttgagtca tatcactgtt catttatttc tcccccttta gttcccgcag
    19261 atctgattct gcaatcaatg cttctaaacg tttagtctgt tgcgccttct cctcttctgt
    19321 ccattcaaaa tatccagcaa ttgcatcgat cacaggttgc ttgatctcat caagctgatc
    19381 tcgttcaaac aacagatgat ttgtacgacg aatgagataa tcgcctgggg ccaaaaccat
    19441 ttcttcttct aaaccataac gtaaccgagc tgactcagcg agactcaaac caggataagg
    19501 tgccatttct tttgctaatt caaagatacg tcgggcatta gtcccgtaaa aatcagcgat
    19561 ataagtagca tcttcttcct ctaaaccggc tgctactcct tcttttgcta attctgtcac
    19621 tgtttcttct aatttagttg gatcgaagtt tccacctgaa atctgatatt ttttagagtc
    19681 gatttctttc gtctctattc cgtattcttc ttttaataat tggcgtatca atcgcaaagc
    19741 tccttctgcc atcttacggt aatcggtgat tttcccacca gataaagtca gcaaaccatc
    19801 tggttctctt tctaaagaac tacctctgga aatcgtagaa ggtgcttttt catcacggct
    19861 gttttccaaa tggttcaaca cgtcttctac ttcagcacga gaaactttgt tttccttata
    19921 ttcacttact gtatcgacaa ctttattgaa gctcttgtct gaaatcgatc cattatctcc
    19981 accattgtaa tcagaaccag aattaccaat caacagtgga cgaagtcctg cccagcttgc
    20041 ttcgatatca ttcaatgtga tatttgcttc tggatagcga tgattgatca catccaatag
    20101 ataatctaca tcttcttgtg tgactttcgg atccgtaaaa tctccttggt aatccgtatc
    20161 tgtcgtacca aagtaagtct tgttttctct tggaatagca aaaaccatcc gcccatcttg
    20221 ctttcctgta tcgaagtatg tgggttgcgg tacaggcagt tttttcgcat ctacaactaa
    20281 atggatccct ttagttggac gcattttagg agagactgga cgcgtaaaat tcaagtttcg
    20341 tactttatct acccaaggac cactcgtatt gatcactaat tttgctttga tttcgatcac
    20401 ttcatctgtc agcaggtcac gagctttgac gccaacgatt tgatcgccat catataaaaa
    20461 gccaaccgct ttcattttac ttactagata agccccatct tcggcagcct ttttgatatt
    20521 atcaatcact aaacgggcat cattgttgcg gaaatctaga tagacacccg cgccttttag
    20581 cccttctttt ttcaaaaacg gttctctttc caatacttct tctggtgtca gcgtataatt
    20641 ctcatatttg gttcctgtca catttgccag tttgtcataa aggtccattg ctactttgac
    20701 cgagaacata ttgaaggttg ttgctccttc gccctcatag attggcaaaa gcattggatc
    20761 tggttttggg atgtgtgggg caatactttg tacgactgca cgttcaccaa ctgtgtcagc
    20821 tactacttcc acatcaaatg tcttcagata acgaataccg ccatgaacaa gtttggtcga
    20881 gcgagaggaa gtcccttctg caaaatcctg catttcgatc aatcctgttt tgattcctga
    20941 tgctgctgac tggactgcca caccggcacc agtgattccg ccaccgatga tcaacagatc
    21001 caattcttct tgctgcattt tttgaatgct atcttgtctt gtcttgtttg aaaacatatg
    21061 gatttcctcc ttattcaaat gcttgggtag cttttactgc ttttttccaa ccgttgtaaa
    21121 gtttttctcg gcgctcttcc tccatttgaa cttcaaatag cttgccagct tcataaaatt
    21181 cgcggatctc gtctgtatct ttccaataac cgacagctaa tcctgctaaa aatgctgccc
    21241 ctagcgccgt tgtttccaag ttttctgctc gttggatcgg tacattcaag atatctgttt
    21301 ggaattgcat cagatattca ttgtttgctg caccgccatc tactttcaat acaggaatct
    21361 tgattccagt gtcatcttgc atcgtatcga tgatatctct cacttgataa gcaatcgact
    21421 gtaaagtagc tttaatgata tcttcttttg tcgtaccacg tgtcaagcca aacatagccc
    21481 ctcgtgctgc ctgatcccaa taaggagcac ctagcccaac gaatgcagga acaacataaa
    21541 cctcatcttc gcttgtagag cgtttcgctg cgtcttctga atcgctagct ttttgcaaca
    21601 tttgaagtcc atctctcagc cattgaatcg aagaaccagc cacaaaaata cttccttcta
    21661 aagcatagta aacctttcca tttatgccgt aaccaattgt cgtcaataaa ttatttttgg
    21721 ataattgcgg tttttcaccg gtattcatga caataaatga acctgtacca taagtattct
    21781 tgaccatgcc tggttcgaat gccatttgtc cgaataatgc ggcttgctgg tctcctgcca
    21841 tccctgaaat tggtgcttcg cttccataaa aatgataacc ttgcgtataa ccataaactt
    21901 ctgagttaga agtgactttt ggtaacatca ctcgaggaat atttagtaaa tctaaaatct
    21961 cctgatccca atctagctga tggatattga acaacatcgt ccgactggca ttcgagtaat
    22021 cagtaacatg tgcttgtcca tcggtcagtt tccatactaa ccaagaatcg atcgttccaa
    22081 acatcaattc gcctttttca gctcgttctt gcgcgccttc tacatgatca agaatccagc
    22141 gtatcttcgt tgctgagaaa taggcatcga tcactaaccc tgttttttcg tggaaaaatt
    22201 cttgatagcc ttcatttttt aagttatctg caattccagc tgactgtctg gattgccaaa
    22261 caactgcatg atagattgga cgtcctgtat ctttttccca tattactgtt gtttcacgct
    22321 ggttcgttat gccaatccca gcaatctgat ttggtttgat ccctgattcg atgaacgctc
    22381 cggcaataac tgactgtaca gaattccaga tttcatttgc atcatgttcc acccaacttt
    22441 cttgcgggaa atattgcgtg aactcttttt gagaactgcc gatgtgtctt cctttcttgt
    22501 caaagataat tgctcgtgag ctagttgtac cttggtcaat cgccatgata tacgttgatt
    22561 ctgtcattgt gattccctcc attatatgca aacgctttct ttaactactc ttatcatact
    22621 actgttttaa aaaaagaaac tgtcaaaatt ttattctttt tttctttttt tgacagtttt
    22681 ttattatgaa gctatttttt aatcaattct tttctttttt cagcgtctct attcttcttt
    22741 tcaatcgaat aatgtcgtaa ggtgacgcaa attctgacag ataatattca cactgttttt
    22801 tgtttctctg gcaaaatgtg atcaccaaat catattcttt accttcttca taacttccaa
    22861 tctcaatacc gccgattccc ctcagttctc tgatcagata ttgttgaaaa gcaatacgat
    22921 aaatcggcag actttctaaa tcgattccaa tcactactgg ttttgcaata taaaaatcga
    22981 tcatcaaaag aacattggca tactggatca tcaggtaact aagctcacta tcttcagctc
    23041 ttttcctcgg caattgctgc accgctgttt cctgtaattt cgccagtaac tgtgttaagg
    23101 aatgcccgag cattttctgc tgctgttcca aaagccgatc acgctcatag acttcgatct
    23161 ttccaacaaa gaaaaaccat tcattgttga tctgggaaat ttggtagcct actgcctttt
    23221 cttctttgat tttcaaacga cgaggacgat aatgccataa catcgtttcc cgaatgtaag
    23281 tatctaaaac agcggttggt aactttttgc tccgtgtcaa atcatactga taaaatattt
    23341 cttcgtctac tatcgaaaaa gaaacaaaaa agctgtaaaa catcattgtt tcgtatacat
    23401 tcagttctgc tgctgttcga ctaagatgca tcacgatgat tggatcgatt gcttgataaa
    23461 gccggtccga ctgatagagt aatttctttt ctttcaaagt tgcgtacgta cttttttcat
    23521 tcaatagtct ttttttcgta atcccgagcc agcaagcaat ctgtgcttct gctgatgccg
    23581 taaacgttgt ttctaataca cggtccaatc ctagaatgaa cggtcttttt tctggtgtat
    23641 tttgcaaaaa aagcggtcgc tgatcttccg ggatatattg gaatagttca taataaaaat
    23701 atcgtatctg tagctcttct ccgtataatt gaccgttttt gatttgtaat ccaaattcct
    23761 ctaagagctg gttcaattct ttgatctttc gaaaaacaga agactcactg atattaaacg
    23821 ctgtcgctaa tcgaacgatg gatacttgct gatgttctaa taaatagacg agcatcttaa
    23881 acttcaagct ttcctgcaat aaaaacaata gaattttttc caaacttaca tcttcgtcca
    23941 acacaagcag cgcctgatac tcatttcgct gtacttctac ttttttttcc atcattcggc
    24001 caatcaattc aatatcttcc aaatattgat caaacgaggc ttttgacagt tggacatgct
    24061 ctctcaaacc attgatagag gctgttcccc cttgcagaac aaggtttcgc aaaatcagga
    24121 tctgtaactg ctcttttctt tctaataatg cttcaatctt cataggcagt attcgccacc
    24181 tttgaccaca ccatatctgg cacacggtat tctggctgga aaaatagctc atgttcttct
    24241 aactgttcaa acgtccatcc ttcttctact gcaattgtca gttggtcaag aaggtggaac
    24301 atctcttttt tgctttggag ctgtgctcca agaatctttt tttcagaatc aaatagaaat
    24361 ttgagtttaa ctttgctttg atttattgct gtaagcttgt agtctacgta tcctgtttca
    24421 attttttggg gataaaaaat gctctcttct tctgtcaaac ctacgcttcc taagaaatag
    24481 ccaaaccact gattgccgac tggacgatag gttccctgat cctttgtctg tgagccacta
    24541 attgtcttgc tgacatttcg tgctgtacgg acagcgttat ttactagaga aacatacatt
    24601 ttttcatttg ttggacgaaa agttacttgg atcgcatcgc caatcgcata aacgtctttt
    24661 tgagacgttt gcaagtagtc atctacccag atcgttccat catcattcaa tgttaattgt
    24721 tcttcccaca tctgattgtc gggacgagaa tgattagcca gcaaaacaag atcatagttc
    24781 tcctttcttt tttccgtctc tacaatagcg gtttcatttt cggtcaaaga cacaacttgt
    24841 tcttctaaaa aaagtgaaag cccttgattt ttcatttcct ttactaacgg ttctgtcatt
    24901 tcaggatcta gataacgaaa aagaattgac tttcggcttt cgtaaagatg gatttgtttt
    24961 tttcttgatg ccaacccttc agctatttcg attcctgctt gtcctgcacc aataattgca
    25021 attttctttg cattcatgat cttttgctga agagccgtta cgttttgtat ctttttaact
    25081 gggtgaataa gcgttgattc tgcagaaata tttcgaaact tttgatttga accagtcgct
    25141 agaatcaaac gatcaaaatc caattttttt cctcctgcta aaacgacttt tttttctttc
    25201 aatcctatta ctgtcttttc tgtgtaaaca tggattcgat agcggtcagt cagttcttct
    25261 ttcgtgatcc aagaaagttg tttttcactg gtaacctttc cttttaatat caaattgaac
    25321 ccgctgggaa tccatcctaa cttcgattgc ttttcaatca aatatatttc acttgttggc
    25381 atcaattggc gaagataaat agccgcatga atccctgcaa acgacccgcc gacaattaca
    25441 atgttcatct gcattcctct ttcttataag gtgttctact gctaacttct caatcagaaa
    25501 acacagtatt cataactcgc tttcaacctt atgatacata gcgatgcagc agaatcaacc
    25561 ctctccatca taccgtgttc taggtctgct tctcaataat gtactagtgg acctaattga
    25621 agagtatcat ttctgaagag atgtttcaaa aaatgtactc ttataaaaga aaaaaactcg
    25681 ccttttacgt acctctgtcc aaataggaaa cagaaaagaa cgaaaagacg agttttagcg
    25741 tgtgtaatgt aatagttaga atagacacat catttatttt taattcttta atgcttcaac
    25801 aactgcttcg cctaattgat gagcagattg aggattttgc cctgtaagaa gccgcccatc
    25861 aatttgaacc acttctgaaa aagctgctcc agactgatag tttgctcctt tttctttcaa
    25921 agcatcttct gctaagaatg gtacttcatt cgttgtacca tttgcttcct cttcttcatt
    25981 actgaaccca gtcactgtac gtccagctaa aatagatttt ccagactcat ctgtaactgg
    26041 caacaatcct acaacaccgt gacaaacagc tgagaccagc ccttcatttc gatagatttg
    26101 tgaagcgact ttcccaagat ctattgcttc tggaaaatcc cacattgttc cgtggccacc
    26161 agcataataa accgctgcat aatcatttgc atccacatca tcaggacgta aggtatttcc
    26221 caaggcccga tttctgaaat cttcatcttc ataataggcc caatcgattt catccatatt
    26281 caaaacactt tgcggttcta aaggtacata tccgccttta ggactgacaa aatctgcatc
    26341 gatcccattt tttactaata catcataaaa atgagtcagt tcacttaacc acaaacctgt
    26401 aggacgctcg atcttttcgt acttttctac atttgttaat acgatcaata ctttcatttt
    26461 cccatcctct ttctataaaa attgcgttat tgataacgtg ctttcaagaa attatataat
    26521 gggcttggca aataaagctc tttatatgct ttttcaaaag gtttcgttac aaattcatcc
    26581 tcacggtttt ccaataattt ctctgcccag tcaggatcga cgatcatttg ctgtccaatt
    26641 gctgcaagtt ctgcatattt caatacctgc tctacatctt cgcgattgcg gatcccaccg
    26701 acaccaacta aagggatctt tccttgaatc gtgttatgga ccacttcaag aatcgattta
    26761 tcgttgtatt tatcagaacg agctattcga tcatacatat ttaatgaaac atgcagataa
    26821 tctagttttg actcacgtaa tcgttcaaga agccagatcg tgtcttcaaa acggatacct
    26881 ggtgtttcat attcttctgg tgagaaacga taccctacga tgaatggttt tttagcatat
    26941 tgttcaaccg ctgagaaaac cgttgttaat aattcttcaa taaaacgata acgattttcc
    27001 aagctaccgc cccattgatc ctctcgtcta ttcgaatgcg gagagaaaaa ttgttggatc
    27061 aaatacgtat ttgctccatg caactcgatt ccatcaaatc ctgattcaat cgctctacgt
    27121 gtggctttgc caaatgcttg gattgtttca acgatttctt cttcactcat tgctcgtggt
    27181 gtttccgcat caggacgttt tgctgcaact gcacttgcag atacaggctg ttctccagat
    27241 aaagttgctc gacttgtcat tcgtccaccg tggaaaatct gaataatcgc ttttgcgcct
    27301 tctccttgga ttgctttcgc tagctcactt aatcgaggaa tcatttcatc atgagcgatg
    27361 ctgagttcac ctggccagcc ttttccgata tcttggacat ttgcagcacc agtaataaca
    27421 gcaccaagac ctttagaacg ttgtttataa taagtgatct catcttcggt gatcactcca
    27481 ttgaaaaagc tgagttgtgt cgtcataggc gacagcatca atcggttctt caattgaact
    27541 cctgaaggta aagtaattga ttgatcaaat tgtgacattg tcattcctcc cataattcgc
    27601 tctgattaac ctatcgagaa tcatctatca ttaaaccact cgattagttg tacatgcaac
    27661 taatttgctt aaaaaatttt attttttttg taacttatct gttgcatcat tcattgtctg
    27721 ggctaattct ttacttgctt catttcccaa aactgctttg tatgactgat gaagttcttc
    27781 ccagctacca tcgacttcct tagcaaattg gcgtccttgt tctgtaagat aaactaatga
    27841 caagcgacct tcgctaactg ttttgacgta tcctttatat tctaattttt caacaaaacg
    27901 agcaatcgtt gatggggcgg aatagagcat ttgtgcaata tctttttgtg gtattccatc
    27961 tttttctttt aatatcaata aaataaaacc ataagttggt gccagacctg tccttctaaa
    28021 cgcttcatca gctaatttat tcaacatgcg atctagcttt ttcacgctaa agaaaagaca
    28081 ttcatctaac gctttttcaa ttggcttttc catttcatcc cctccattgg cgtacatatc
    28141 atagtatgat ttttattttt tgtcaaaaaa cacggaataa tgtccgcctc ctctgtctag
    28201 atttgaaaaa aagaagtagc catcttcaaa gaatttcggc ttgttttcga caaaactcct
    28261 tctggaacac cgtgaatcag aggttgtgaa aaagtcgttt agttttgagt atgaagaaaa
    28321 attttgtgaa aatggctttc cacatttttg cataaatttg gcttaatacc gaaaaactgg
    28381 cttttgaaca ccgtttatat cagaggttgt gaaaaagccg tcctgcatca agtaataaga
    28441 acagccaaac aaaaatagtt cctcatattt tgtcgttttt tgagctaatg ccgatggtca
    28501 gctgattgta caccgtttat tgagaggtcg tgagcctgcc gttgaactcc gagtcacaag
    28561 gagcaatttc aaaaaacagc ttctcatgtt ttatgactat ggtgacttgt gatcgaggag
    28621 ttggcaggag aatactgttt attcgtaaag aaggggctgt gacaagactt ttgtcacagt
    28681 cccttatttc cttttattat aggatttcgc catttgattg gatgacttgt ctgtaccaat
    28741 caaatgattt tttctttaac cgtttctttg tgccattccc ttcgtcatct tgatcaacat
    28801 agatatatcc ataacgtttt gacatttgtg aagtagaagc gctgacgata tcaatgcagc
    28861 cccacgacgt ataaccaaat aaatctactc catctttgat agcttccttc atctgctcaa
    28921 tatgctgctt caaatagtca atgcggtaat cgtcttgaac ggttccatct tcttctactt
    28981 tgtctatagc tccgagtccg ttttcagcta tgaacagtgg tttttgatac ctatcatata
    29041 attgattcaa agcaattctc aaacccttag gatcaatctg ccagccccat tcacttgatg
    29101 gaagatacgg atttttaatt cctccagtta ttaagtttcc ttggacccct tcccaatttt
    29161 ctggatgagc actgactgca ctagacatgt agtagctaaa agaaacaaaa tcaacagggt
    29221 aggctgctaa gatttcttca tcacctactt ctatttttaa gttgacacct ttttcttcaa
    29281 acattcgttg cgtatacgct ggataatatc ctcttgcttg aacatcacta aagaacaaag
    29341 aagttctttg ctgctcaatt gttttttgaa tatcatccgg atcacaagaa tacggaacaa
    29401 gaagctgata gctgatcata caaccgactt gtgaaccttt gataatttca tgacaagctt
    29461 ttgtagctaa agcacttgct ataaactgat ggtgtgcacc ttggtatttc gcttgttcca
    29521 aatggggatg gttttcttca attactccta cgctaacaaa agggtgatgt tttacacagt
    29581 tgatctcatt gaaagtcatc caatatttta ctttattttt atagcgtttg aatactgttt
    29641 cagcgaaacg aacgtaaaac tgaataagct ttcgattagg ccatccacca tattctgtaa
    29701 ccaaatataa cggcatttca taatgggaaa gagaaacgac tggctcaatc ccatatttta
    29761 gacattcgtc aaacacttga tcataaaaaa ataatcccgc ttcattgggt gtttcttcat
    29821 caccatgagg aaaaattctt gtccatgcga tagacatccg atagcattta aaacccattt
    29881 cagcaaataa acggatgtct tctttaaagt gatgatagaa atcatttccg tgtcttttag
    29941 gataataata cttgctgttg ggatcctgcg ccgcttttac ttgctgatgc gtcatcatat
    30001 gccattgatc tgtccatttt tctttaggta aatcgctgtc gaatgtatac acatcagaca
    30061 cagataatcc tttttcatct acatcccatg caccttcaca ttgattggcc gctgtcgctc
    30121 cgccccataa aaatccttct ggaaattttt tcatatcgat ccccctaaac ttgctagttt
    30181 tcttgtttga ttatttttct tatgactagt aatgatattt ttgaatctac tactatttgt
    30241 aacaataacc attgtggtcg gatcatatcc ctctttgatt aattcttgca aatctacttc
    30301 catcaataaa tcgcctttac gtactttttt tccatcagat actgcacttt gaaagtattt
    30361 gccatttaaa ttcactgtat caataccgat atggatcagt atctctgctc cttcttcact
    30421 gactacccca attgcatgtt tcgttggaaa aactgttgaa acttccccgt ttacagaact
    30481 gtagattttt ccgtccgttg gaatgatcgc aaacccttct cctaaagatc cactagcaaa
    30541 tgttggatcg tttacttcag ataaatcaat tatttcacct gctgcaggag tacttaacac
    30601 tatatccttt gaggattctt cttcgatttc atccttgtag ccgaaaagaa aagttagcat
    30661 acaggaactg atcatcgcta agaaataact agcaactagc caaacaaatc cttcaccaat
    30721 ataaatcggg aaagacaaaa tacttcttgt agcaactgcc aaagcaccgg ctcctgcggc
    30781 accagctact ccaccaaata tagctccaca tatacaagca atgtaaaatg gtcgtttata
    30841 tttaagagtc acaccataaa cagcaggttc agtgattcca aataatgctg tgataaatgc
    30901 agataaagat aaacttttta ctttcttatt tttcgttttc aaaaataccc caagtactgc
    30961 tcctgcttgc gcagctacgg cagtcataca tgctggacct agcgtatcac gaccgtagaa
    31021 agaaagattg ttggtcatta ctggtacaag tccccaatgt aacccaaaaa tcactaacac
    31081 ttgccaaaat ccgccgataa cagcaccaga taaaacaggg ctaaagttat aaacagctaa
    31141 atatccagct gcaatcagct gacttgcata atctgcaatt ggacctacaa ctaataaaat
    31201 aattgggacc atcacgatga tacaaatgaa tggtgtcaat aaatttcgaa tactttcgtg
    31261 caagcttttg cgaatcttag gctcaatata agaaagaacc catacagcta aaattgctgg
    31321 aataaccgta cttgtatacc ttgctagaat cacgggcatc cctagaaatc gcaaggttat
    31381 gctatcacta tatgcactga caatcgtagg ataaacgaga gctgctgcaa taaccatagc
    31441 gatgaaacga tctgtattga acttttttgc agcagtatat gccaaaaata taggcaagaa
    31501 atagaaaaat gcatcagctg ctgcattcaa aatgatataa gttcctgatt cagtagttaa
    31561 taaattcaaa ctcgtacata aggcgagtac tcctttgatc aaaccacttc ctgccaagac
    31621 gcctaaagtc ggggcaaaaa cagcggcgat catgtctata aaagagttta aaatgccttt
    31681 tttctcagtt cctctctttc cactagatgc atcatcttct agtttcattt gtttggataa
    31741 tacgtcatac acttcattta ctgcatttcc tactacgact tgaaacattc ctccagcttc
    31801 gactacagtg atcacacctt ctagttgctc taccttctcc ttatctgcct tcttattatc
    31861 ttttagggta aagcgtaacc ttgttgcaca atggtaaaca ttgctgacat tttcttcgcc
    31921 tccgacaaat cggacaatat ctttcgctaa ttgtgaatag tccattttta cttccccctt
    31981 gttaattaat gccgtagcaa tccttcttac aagataagta tagttcttag aaaacggttt
    32041 ctccattcaa ttagtacccc ttctaatagc gaacgaaact gtacaatagt gtgtatggtt
    32101 tacttagccg agcaaatcac tcactgcttg aaagaaaaca agtatatcgt ttctcttata
    32161 gtcaaaggag acaaaaaaaa cacgcttgat cttcctttgc tgatgtataa gtggtatctc
    32221 aagctgatac tctgtgattt ttgtttctac ttggcttttg ataagacagc acaacaaagt
    32281 ctcggcattt aacggaagca taactaggtc agaactagga gagattagtt gctgaatgga
    32341 atctatcttt ttatggattt ttatttgatt ttcttctttg tagtaatgat caagcagttt
    32401 tgttttcttt ttttcttcaa ctatggtcag cttttcacgc aaaatctgat aaatctccca
    32461 agattcaaac tcatctagaa cactaggtag ataaatcaaa tcatcccact caaaaataag
    32521 ctcttgctca gtagtcaaaa ttgcatcata ttccgttatt ttgtttgatt gatttttgaa
    32581 aagatacact ggttctatcc ggcattcttt gatccacatg ttcatacttt gttgaatctg
    32641 tttttttagt gtattcaaaa tactaattgg ctggttacta acaatcagta tctccccttg
    32701 ttttcgaaaa ggattcaacg cttcggcaag atacaaaaca aaataagcgc tttcagctaa
    32761 attgggacgt aattgaaaat gtattgggaa gaaacgccga attaaataaa tttctaaagg
    32821 ataacgggag atgatctttt gtacaaaatg attcatggta ttatgtccgg catccatcct
    32881 cgttttcatt tgtttgatat gggttagcaa aaattctgtt tcatggaaca ataacgaaac
    32941 aggaagatct aaaaaacgaa taatcgtttg ctcaaatgct cttagattct tctctatttc
    33001 tttgtttttt tcatcattaa tcatcaaata atttaactct aatagtcgat tttcaattgc
    33061 ttgtttttct tgattggaaa aagaataggg tagttctgat gataactttt ttgtgagggt
    33121 ctctaccatc ggaataaata atgtatacct gtttgttttt tctaaaagtt cttctcttcg
    33181 aagaatggtc aaaacgagat accgagaaaa tcgtatgaag tcctctcctg aaacaatata
    33241 attatatgat ataagaacct cttttaatgt cttttggaca gtcggcatga tattggaaaa
    33301 aattgtactg atttgttgag gaaaccttgc gtgttggatc accgtttctg ttccaacaag
    33361 agataagaaa attctttttg aattttcttc agcaatgatt tttagtcctt ttgtcgacga
    33421 aacttccagt tctaatttag gattgcgctc gatccaacgc aaaatcgttt ttatttgtaa
    33481 tgatacggct gtttttgaaa gaaagaaaat gtctgctatc ttttgcatac tgacataccc
    33541 tttttgaaac aataagtacg tcacaatagt tgaagctcgg aaatccatgc tggaatagat
    33601 tttatgtttc gtcacctctt gtatgatttg ctgtttagtt tcagattcaa ttgattctaa
    33661 aaaataacct gaacgtttat ttgactggat aatgattttc ttctcgtaga tagcaagcat
    33721 atttttgatt tcagtttgga tcgttttctt gttggtacct aaagcttcag ctaattcttc
    33781 gcttgttatc caatgattct tatttcttaa tactacaaga atttggagtt gtcgtttatt
    33841 taagtccatt tgcttacacc tcctctttgt tataaacata caggaaaaac ctctcaaatg
    33901 acaatagtca cttgcagagg tttaaaaatc atttgaagaa agatctatat tcaaggtttt
    33961 taccattaga ctggataatt tgtttataat attcaaaact atcttttttc aatctcctaa
    34021 agcttccgct cccatcattt tgtctatcca cataaataaa gccatatcgc ttcctcattt
    34081 cccctgttcc tgccgatacg atatcgattg gtccccacca taagtaacca atgatttggc
    34141 aaccatcttt tattgcctca gcaatttcct gaagatgtat ttttagatac tcacaacgtt
    34201 ttggatcgtg gattcgattc aattcatctg gtgcttcatc tagtccaatg ccattttcaa
    34261 ctatgaataa tggtaattca taacggtctg tcaattcatt catgacataa cgaaacccta
    34321 gaggatcaat tggccaactc caaggttcgg gagatttttc cttcaaaaag ggattatcga
    34381 aaccatttgc tccgccagta tctacgcgca tttctgcttc ttttttatag acagcgctac
    34441 gataataact aaatgcaata taatctgccg tgttagcttg caagcactgt tcgtctccat
    34501 ctaacattac tggtgcacag tcttcttcct tccatgtcct gtaaatataa gctggataat
    34561 gccctcggca catgacatcg gagaaaaacc atgaattgcg tttaaattgc agtgaaccaa
    34621 atacattctc tggatcacag tcatatgcat aagtcgctaa tgcagaaaag ctacacataa
    34681 tacccatctt agcatttgga tcgattttct ttaataattg agtagcacgg gcgttcgcta
    34741 gaaacatatg atgacaggct tgataaatca ctttttttgt tagtgtagca atgggttcaa
    34801 gtttatctct agcagggaat gtaaccaatc cagcagctgc ataaggcata cgaaaagcat
    34861 tattgatctc attaaaggtc atccaatatt tcactttccc ttggtagcgt tcaaagacgg
    34921 tctgcgcata aaattcaaaa aagtcgatta attttcgatt ttgccacccc ccatactctc
    34981 ctaccaatgc taagggggtt tcataatgac taagagtaat tactggctcg atatcatatc
    35041 gatttaatgt attgattagt ctgtcataaa atacgagacc agcctcattt ggcttctgtt
    35101 cgtctcctct tggaaatatt cgtgcccatg agatgcttgt tcgaaaagct tttaaaccta
    35161 actccttcag taaacgaata tcctcttcaa atcgatgata aaaatcaatc gcttcatgag
    35221 aaagataatg gagtgattcg tctaaagcaa tctcccaaat ctttttttct tcattccact
    35281 gaatactagg atgctggttc atgatcccga taagcgtatc tgtcacatta ggtaatttgc
    35341 cgtcttccaa ccatgcacct tcacattgat tagcagctac tgctccgccc cataaaaaat
    35401 tttctggaaa tatttcctga tccataacta tctctcctcg ctatcaacaa tttcttactt
    35461 gaagtatagc tcgattcatc aaaacaagat attctaacta tttcggcaga agagaagaaa
    35521 aaaattgaat gctactcttc atttttaata tttatatata acaaatacat aagacgatcc
    35581 ccgaaagaaa acactcgtta accatctaag caaacgattt attttacttc caacacaaat
    35641 gcttcataag gtcgtaaatc aactttgccg tctggtaact gtttcagagg ataatttgac
    35701 acaataaccc ttaaagcttc acgtggaata gtgaacgtct gcttttcact ggacatatta
    35761 caaataacga gccattgctg gtcttcatat atgcgagaat aggcaaagac atgctcaggc
    35821 gtcttttcca ctaattcata gtctccccag acgatgatgt catgttttct acgcaaatcg
    35881 atcaatttct tgtacatata aaaaacagaa tcttgatctg ctaacgcttc ttctgcattg
    35941 atttctttat aatttggatt gactcgaagc catggaattc cagtagtaaa cccagcatgt
    36001 tcacgggcgt tccattgcat aggcgtccta gcattgtctc ttcctttcgc cttgattgat
    36061 tccaagattt cctcttttgt aaatccagaa gaaatccttt cgttatacat attgattgtc
    36121 tcaatatctt ttgcttccgc gatatcatcg attgggcaat tcgtcatgcc taactcttct
    36181 ccttgataga tataaggggt gcctttcatc atatggagca agatagcaaa catcttggca
    36241 ctttcttttc gatacacatc gtcatttccc caacgagaaa caattcgcgg aagatcgtga
    36301 ttattccaaa ataaactatt ccatccttca tcacctaaag atgtttgcca tttggataat
    36361 acttttttca gttcagagat actcaagggt ttcaaatccc atttgtcttt gccttcttgc
    36421 tgatctaatc ctatgtgttc gaattggaag accatggata attcatttct tttaggattg
    36481 gaataaagtt tagcgatttc ggtagttgct ccccatgttt cgccaacagt catcagattt
    36541 ttgtcaccaa aagttgcttg attcatttcc tgaagatact catgcagttt aggtccatta
    36601 cttgtgattt ctttatccgg ttctttgcca atcatatcga tcacgtccat acgaaagccc
    36661 ccaatacctt tatcgatcca gaaattcatc atttgataaa tctcttggcg aacctgttta
    36721 tttttccaat tcaaatcagg ttgacgcttg ctatacaagt gcagaaaata ttgccccgtt
    36781 ttttcatcca actgccaagc agaaccactg aatattgagc gcaatctatt aggctctttt
    36841 ccattgaccg gatcacgcca aatataatag tcccgataag gattttcttt atttttacgg
    36901 gcctcaatga accacggatg ttcatcagaa gtatgattca ccaccagatc catgatgata
    36961 cggatatttc ttttctttgc ttcttcaatc agttcttcca tttcttccat cgtaccgaat
    37021 tctgccatga ttgcttgata attactgata tcatacccat tgtcatcatt cggagactcg
    37081 tatactggag aaagccaaat tgcatcaata cctaaatacg ccagataatc caacctggaa
    37141 ataatccccc gcaaatctcc aataccatct tgatttgaat cttgaaaact tcttggatag
    37201 atttgataaa caactgaatg gtgccaccat ttttgttcca tgattttcct cctataaatg
    37261 gatgtttcta ttttttgctt aatcatttat tttagtctct ctcactaaaa attgtaacgc
    37321 tttcaaatag ctattgcacc ttgttatcgg taacagaata aaacaaaaaa acagctgagg
    37381 aaattccctc aactgttttt tgaaaggatt gatttatgca gggaagaatg ctgatttcac
    37441 aacttcttta taaacggtgt tcactcagct gtcccttggt attagctcaa aaaacggcaa
    37501 aatataagaa actatttttt cgtttttttc tctaatactc aggccagaac gctgatttca
    37561 caacctcttt atttcacatt caatatccac atgaatccca caaatacaaa gaacagacca
    37621 tacatgattg gagatacttc ttttcctctt ttagctgcta acatggtaat tggatagaag
    37681 ataaaaccta atgcaatccc atctgaaata ctataagtca aaggcatgcc gatcaagatc
    37741 aagaatgaag ggatcgcaat ctctaagttt ccccattcga tttgtcgcaa agattgtgcc
    37801 atcaatactc ctaccataat caatgctggt gcagttactt gtgaagtaac aactgatagt
    37861 aatggagaaa agaacaagcc taacaagaag aaaatccctg tagtaatagc tgtgatacct
    37921 gaacgtcctc caacggcgat accagctgat gattctacaa atgcaccaac aggagaagta
    37981 ccaaataagg aacccgcaag catagctgtt gaatcagaag ctaaagcttt ccctacacga
    38041 ggcatcttgt tgtctttcat gaaacctgct tggtttgcta atccaactaa agttcctgct
    38101 gtatcaaaga aagtcacaag caagaatgtc agcactacga cccacatttg aagagaattg
    38161 atgtccccta catgttttaa tgcaactaaa aatgttggtt tcaaacttgg tgctgcggca
    38221 acaatttcag aaggcattgg aatcaaacct gtgaccagac ctaaaatcgt tgtagctgcc
    38281 atcccgatga agatgccacc tggtacacgc cgaacaagca agatagcagt tacgactaat
    38341 ccaaaaatcg tcaaccaagt agaaccaacg ttgagtgatc ctaatcctac taatgtagat
    38401 tcattcgaaa cgatgatccc accttcgctc agaccaagga aagcgataaa tagaccgata
    38461 ccgccagaaa tcgcatattt caaatctgct gggatcgcat caatgatcat ttcccgcagc
    38521 ttgaagattg tgatcaaaat aaaaatcaaa gaagcaacga atacgccggc taatgctgtt
    38581 tcccatggaa tccccattcc taaacaaaca gaataagcaa aaaaagcatt gatcccaaga
    38641 gcaggtgccg ttccaattgg ataacgggct aataccccca ttaaaacaca gccgatcgca
    38701 ctggctaatg cagtcgcagt aaacactgct ccttcatcca ttccagaagc acctaataca
    38761 gttggattta caaataagat ataagccatt gagataaatg ttgtaaatcc tgccagtacc
    38821 tcacgtttta ccgtggtgtt caatttttct atttcgaagt aggaaagtat tttttccttc
    38881 atcttccatc cccctaaatc cgaacattat aaataaagtc cgttaaaatt tcgatttatt
    38941 aataaaacaa cgtgttttat catatactaa aacaaaacat aagtaaacaa aaaattcgga
    39001 ttttaagcaa aatccgaacg ttccatctga ttaaatccct ttatctcaaa gttcatgaac
    39061 attcactttg gattacaaga atttctttat tgtgttaata aagtttcttc attctagtct
    39121 caaaaatcaa tagtgtgatt tgattttacc atttcgtttc tgttcttctt tgatgaattc
    39181 ttttgtatcg acaggattca gattatttag tccgccaaaa cctgtatcga ccatttttcc
    39241 ccagcctttt gtcttctttt ttcctgattt gagattcttt gctcgaagct tctcactatg
    39301 ttttgctcgc cgatacttca aataaccttc taatcttccc attagatcca ctgtttttcc
    39361 tcctttttat tcaatcagac gactcagctt ttcctataac aaaataattg ccttcagggt
    39421 cagaaaaatt gaaagtcaag atgccattgt tatctgtgat ttctcctggt tcttccatgt
    39481 tctcgtagat agtttcaaat tcatccgtaa aaaatactaa agaaggcggt ggtcctaata
    39541 cctctggtga aaacttctgc acaaatactt ttgggaatac gcctaattct aattcttttg
    39601 agatgctgag tacaatactc ttaaattctt ctggcaattc gattgtttca gaaatttctg
    39661 caccaatttt ttctacaaag aatcggcaaa tcatctctac gtcattgaca taaagcatca
    39721 cacgtgtttt ttgtatcatc atttgaccct tctttctatt tgattttctt tcctgtctat
    39781 aaactgattc cattatatca cgaaagcgca aacaccttgt gtttgatact attcaagaaa
    39841 aataaagcaa tagtttctag gattgctttc ctccacaaac tttttttaca aaaaaaaaac
    39901 ggcaatgaat gccgctcttc ttatttcgat tcttttataa ttctgcttct gattcatctt
    39961 tggttagtgt gtgtctaaca ctagttacgc caaatgtttg atcgattttt tccttcaacg
    40021 cttcgattgc ctgtgcgcca cgtgccgctt gttcagcatt gatggattca atgaccagca
    40081 ccgctgcttg tgtttcttcc gtcagcatcg ttctttgggc ttcacgccag ttgagacagc
    40141 gacagatcgc tccttgttca tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg
    40201 aatctgcacc taaaggcagg aatgattcgc caccttgggc ttttcctaaa tgcagatttc
    40261 ctgcgatctt ttcgatatct tctccgccgc aaggtacgcc aaaagacaag gaaatactat
    40321 tatagatatc tacaagcgga ttgattggga aaaattcccg ttcttgattt gcacgtttca
    40381 gtaaagcttc aatagaagaa cgtgcacctt ttttagtctt gaatgtggta aatgcctgac
    40441 gccactgagc aatgacttcg ttttggctga atgtttcttc tgtaaggtaa tttcttgctt
    40501 cttttgctgc ctcattcaat aattgatgat acgtttcttc actttctttt gttacgtggt
    40561 tatcgatacc atttacggat aagatttcga tggttgcttc aggaaatact tcccagaaat
    40621 tggtatccac tattactttt ttcatctgtt cacacctctt gattagaata attgcttgat
    40681 gaatagatag tctgtttggg gatcatctcc catataaaaa acatgttcgc tgtatttgat
    40741 aaatcctttt ttacgataaa aagcgagcgc aacatcgttg tgttcccaaa caccgagcca
    40801 aatggctgtt ttttgctttt cttttgcttt ttcaatagct gtatctagta aaaatgtacc
    40861 gataccttga tgtttaaatt tttttcgtac atagattcgc tcgatttcta aaaaatgatc
    40921 agccatttcc tcagattgac tcgttcctgt atttagtttc aggtaacccg caggctcttg
    40981 atcgatactc actaggaaaa aatcggtttc tttatctttt agctcttctg taagtttttc
    41041 tagtgcataa gcttgtgtca agtaatcagc taaatgctcc ggtgaattat cttttccaaa
    41101 tgtatctgta aatgtttcga tactaatttg ttgtagtaag tctacatctg aaggcgtcat
    41161 ttttcgaatt tcgtatttat tcacggcttc ttcctttcaa tagatccttt tattgccttt
    41221 tttgacaaaa ttccaatctt cgctaacatt cttgcatact ttatttaaaa gatattccaa
    41281 ttgttttgct tcttcgtcag aaagaccatt taatgcgact gcattggagt agttgttttc
    41341 tcgaatgatg aaaggataga tttctgctcc ttttttagtc ggatagagtt tcttgatttt
    41401 tttattttct ttatcttcca aacgttcgat catcccattg gattctaatt tcttgattgc
    41461 tcgggcagtc gttgtacgat ctactttgat catttctgct agtttttctt ggatgattcc
    41521 aggattttcg caaatacgta ctaaataaag atactgtcct cttgttaaat cgacttcttt
    41581 gaattcgata ttgctaatag aatctagcgc tcgggcaatc attccgatgt ctcgaaggat
    41641 ttcagccatt tcatcccacc tttattttgt ttgtaagact agttcacctc tttttgttgc
    41701 aaatgcaact attttcaata aaaaaatcaa gttctttttt aataactagt aaagtatatt
    41761 cttttcttct tttacgaaaa agctcaatca caatagatct ggtttttgtt ttctaaaata
    41821 agtaactgtt tcttcgttgt tcgcttttct gatagctttg gcaactcttc tatactaaaa
    41881 aatcccatgt tggaagtttc attattctca ataaacttgg cctcttcacc gtggatcgca
    41941 caagcaaaaa caagtttata atactgaaaa agctgtggaa tatctttttg tttattcgta
    42001 tcaaaaactg ctcgcaactg agtagtttct actgttaatc ctgtttcttc atagacttct
    42061 ttacgcacat tttcttcagg agacaatcct atttcagcaa atccaccagg aagtgaccat
    42121 tctttcgtcc gtttatcttc gattagtagg accttgccat cttttttgat ataggcacga
    42181 acatctactt ttggtgttgg atgtccttct ccaatcgttg tgagcgcttt tatttcttcg
    42241 aaagtttctt ttcctatttc actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa
    42301 cgctctttat caaatacatc ttttccataa tatagaccag cttgagaaag tgcgagtagt
    42361 cttttgtatt gagccaactt cttgttcatt ctcttccctt cttttttagc aaatgtatga
    42421 ctatgtaatc aagtatagaa gactggttta attacggcaa ataaatataa tgaaatctgc
    42481 aataaataaa agaatgttaa aaaactcgag aacaaaagac attcgacttc tctggggctg
    42541 tgaataaatg gcgttcaaaa gcttgcatct gcggtaataa gccccaccaa acgaaaaata
    42601 tgacgagcta ttcttgtctg gctgttctta ttaattgagg caggacagct ttttcacaac
    42661 ctccccacta aacggtgttc tcctaccaac tcctcggtca caagtcacaa tattcatcaa
    42721 acatgagaag ctgttttttt gaaattgctc cttgtgactc ggagctgaac gacaggctca
    42781 cgacctctcg gtaaacagcg ttcaaaagcc aatttttcga tattaagcca tttctttgtt
    42841 acttattgtc attgatttct ttttctaatt tatccaatag tggagtgtaa tccacttgag
    42901 tatagtctgt tacatcaaat tcatatagtg ttcctaactg gaatttgtca tattttcggt
    42961 cggcgatgat ctttttgaat gcttctttcg taatatgatt ggtccctttc gttcgatctt
    43021 ccaatacgtc accatagtga taatgatcca tgatatagct caaatgacga tctgattcac
    43081 gatcacgttt gtaccgacgt tgccataaca cttcaaagtc tgctgttagg cgaatcgtga
    43141 tcacctgata tccgtatcta tctgctaatt cttggagtcg tgctttttgc ttatcactaa
    43201 aaggatactc ggacacaatc actttctttc ctgcttccat atacaaatca agtgctttgt
    43261 aatagaaagg ataaactttc gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc
    43321 cgatggattc tgcatagtat tccttgattt catcgagcgt gatggcatac atatctggaa
    43381 atcgttcccg tagtttattc ataagatatg tttttcctgt accaggacta ccagccagaa
    43441 gaatgaaata ctttttcatc tcatttattt cctaagttaa ttggactttc caagttcata
    43501 aacagtccaa tcttatgctt gacaatttct tttactgctt catttgctgg aatcacatta
    43561 tggcgaagag acttgttgat ttctgtttca ctaaaggttt ctttagctac tttcgtccag
    43621 ttgactaaaa gctccgttcc aacattgaat ttgcggatcc cgttattgat caattcagga
    43681 taatcttctt ctttcacacc tgttcctcca tggataacta gggggacatc aactactgca
    43741 tcgatttctt tcagcaaggg aatgtttact tctgtttttg atttgaattg tccatgattc
    43801 gtaccgatag caatagctaa tgcatccacg cctgtagctt ctacaaattt cactgcgtct
    43861 tccggtcttg tatatacctt gtcattttca gcgacatgga ttccttcttc tgtaccgccg
    43921 attgttccaa gttctccttc tacggaaaca ccgtgcgcat gggcatattc tactactgct
    43981 tttgtcttca ggatattttc tttgaaaggc agatgggacc catcatacat gacagaagta
    44041 tatccgctgt cgattgcttc tttgatatca tcaaagtctc gggcgtggtc taagtgcaag
    44101 actgcatcaa ccatttcttc ttctgcaata gctgtcatta cattgactaa cacatgcatg
    44161 ccgatatatt tagcggtatc gacacttgtt tgaataatga tcggcgaacc catttctttt
    44221 gctgcgccta gcatagcagg aagcatttct aggttatgcg tattaaaagc tccaactgta
    44281 aaattcaact cttctgctgt ttttgttact tctttcaatg ttgtatacat cttttattgc
    44341 caccttatct ttttatttgt ttcctttgat cgttacttgt tttgcaattt gtcgcatctc
    44401 atccattttg cccatgtagt aatcgatccc ttgatcatca ccagcacgag cagcatctgc
    44461 acgcttctgg ttatagaggc tcatcagttt tttataccca tctccgatcg tctgtttatt
    44521 ttccaaactc ttttctaaat attggatggc ttgatcgttc acatacaatt cagaaaattt
    44581 aagtccaact tgttcataaa gtttcgctaa tttatgctta tcttgggtag tggaaatctc
    44641 ttgtttcaat tgtgtaatct ctgattgaat cttttcggtc gcttctggcg atagggcttt
    44701 ttcttctttt gccagaactt ttttcggttc tttttctttt ttcttaaaaa atccaaacat
    44761 cattttccct actttcaatt aaaatgcttt caaaactggg cttaagatcc aggcatataa
    44821 gagtgctgcg gctactgtat ctacatcggt tgctgtagca ttgttgaatc ccatcgaatt
    44881 aaagatcgtt actagcaaag cgggcaataa tgtaataaag aaaccatgag caataccgcc
    44941 aatgatcgct cctcggcgtc cgccaactgc gttgccaaat atccctgctg tccctccggc
    45001 aaagaaattg gttagcatac caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa
    45061 catggcaata atcgtaccaa tcgttgtcgt aatgaaacct aaaatgacag catttgggct
    45121 ataaggaaag aaaactggac aatctaaggc agggatcgca tcaggaacta atttcatagc
    45181 tatccctcga aatgccggca cgatttcacc taacagtaga cgtacaccag ctagcaatac
    45241 atacacaccc actacgaatt ggatggcttg taaaaaggca aacatgatat agttttgatc
    45301 tcctgataat tcagaagcga atggttcgcc tgcaaataaa acagtgatga tatatagcgg
    45361 gaccatcacg accatgacag acaaataagt atcttgcaga aattcgaaat gtgtcggcaa
    45421 cttgatatct tcgatcgact ttttattttc gcctttctca ccaaaaagct tagcaacacc
    45481 ggcttcaaat aagtacccga tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt
    45541 gattttacga atgatcggtt gcgctactgc cggcatagca atggcgaaac aagctccgat
    45601 aaaaccaccg accaagatca atacgatccc tcgcagaccg gcaaaatagc caaagactgt
    45661 cgtcatcgtt gccatccata aaattgcttg accagttaaa aaaatgtact tccattttgt
    45721 gaaacgagcc aaaataatat tgaatacaaa aatggctaag aacgtcagtg caatatctct
    45781 acctaaaccc aaatcattca ttgcttgacc attgatagat tcaatagaag ggatgatccc
    45841 ttgcatatga aatccttcag taaagatttt cccgaaataa gttaaacttc caacgatgat
    45901 gctcgaaccg gcactcaaca cttgaaatcc taggagtgtc tttaacgtgc cagagatgac
    45961 ttgtcctgct gatttcttct gcagtagtag acccaacata gcaatcaatg caatggtgat
    46021 cgatgcctga gtcaaaatat tttcgatgat aaaattgata acgcccatgt t
//
LOCUS       DAFGZE010000018        43016 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.018, whole genome shotgun sequence.
ACCESSION   DAFGZE010000018 DAFGZE010000000
VERSION     DAFGZE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.018"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(133..306)
                     /locus_tag="LTU54_001026"
     CDS             complement(133..306)
                     /locus_tag="LTU54_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295756.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="LTU54_001027"
                     /pseudo
     CDS             754..3116
                     /locus_tag="LTU54_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="LTU54_001028"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="LTU54_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBL8295757.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="LTU54_001029"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="LTU54_001029"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HBL8295758.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="LTU54_001030"
     CDS             5704..6489
                     /locus_tag="LTU54_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8295759.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="LTU54_001031"
     CDS             6502..7353
                     /locus_tag="LTU54_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HBL8295760.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="LTU54_001032"
     CDS             complement(7477..7929)
                     /locus_tag="LTU54_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295761.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="LTU54_001033"
     CDS             complement(8394..8762)
                     /locus_tag="LTU54_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HBL8295762.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="LTU54_001034"
     CDS             8912..9394
                     /locus_tag="LTU54_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL8295763.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="LTU54_001035"
     CDS             9431..10441
                     /locus_tag="LTU54_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL8295764.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="LTU54_001036"
     CDS             complement(10556..11269)
                     /locus_tag="LTU54_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295765.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="LTU54_001037"
     CDS             complement(11459..12049)
                     /locus_tag="LTU54_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL8295766.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="LTU54_001038"
     CDS             12533..12760
                     /locus_tag="LTU54_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL8295767.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13941
                     /locus_tag="LTU54_001039"
                     /pseudo
     CDS             12776..13941
                     /locus_tag="LTU54_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            14175..15218
                     /gene="hrcA"
                     /locus_tag="LTU54_001040"
     CDS             14175..15218
                     /gene="hrcA"
                     /locus_tag="LTU54_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL8295768.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15312..15875
                     /gene="grpE"
                     /locus_tag="LTU54_001041"
     CDS             15312..15875
                     /gene="grpE"
                     /locus_tag="LTU54_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL8295769.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15926..17755
                     /gene="dnaK"
                     /locus_tag="LTU54_001042"
     CDS             15926..17755
                     /gene="dnaK"
                     /locus_tag="LTU54_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL8295770.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17906..19072
                     /gene="dnaJ"
                     /locus_tag="LTU54_001043"
     CDS             17906..19072
                     /gene="dnaJ"
                     /locus_tag="LTU54_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL8295771.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19154..19819)
                     /locus_tag="LTU54_001044"
     CDS             complement(19154..19819)
                     /locus_tag="LTU54_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295772.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19795..19968
                     /locus_tag="LTU54_001045"
     CDS             19795..19968
                     /locus_tag="LTU54_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295773.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20196..20402)
                     /locus_tag="LTU54_001046"
     CDS             complement(20196..20402)
                     /locus_tag="LTU54_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295774.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(20428..20847)
                     /locus_tag="LTU54_001047"
     CDS             complement(20428..20847)
                     /locus_tag="LTU54_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8295775.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            21351..21704
                     /locus_tag="LTU54_001048"
     CDS             21351..21704
                     /locus_tag="LTU54_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8295776.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(21764..21931)
                     /locus_tag="LTU54_001049"
     CDS             complement(21764..21931)
                     /locus_tag="LTU54_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL8295777.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(22050..23969)
                     /gene="tet(M)"
                     /locus_tag="LTU54_001050"
     CDS             complement(22050..23969)
                     /gene="tet(M)"
                     /locus_tag="LTU54_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL8295778.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(23985..24101)
                     /locus_tag="LTU54_001051"
     CDS             complement(23985..24101)
                     /locus_tag="LTU54_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL8295779.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(24346..24423)
                     /locus_tag="LTU54_001052"
                     /pseudo
     CDS             complement(24346..24423)
                     /locus_tag="LTU54_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(24577..25212)
                     /locus_tag="LTU54_001053"
     CDS             complement(24577..25212)
                     /locus_tag="LTU54_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8295780.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            25724..26866
                     /locus_tag="LTU54_001054"
     CDS             25724..26866
                     /locus_tag="LTU54_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL8295781.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            26859..27065
                     /locus_tag="LTU54_001055"
     CDS             26859..27065
                     /locus_tag="LTU54_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBL8295782.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            27144..28337
                     /locus_tag="LTU54_001056"
     CDS             27144..28337
                     /locus_tag="LTU54_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8295783.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(28508..29245)
                     /locus_tag="LTU54_001057"
     CDS             complement(28508..29245)
                     /locus_tag="LTU54_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL8295784.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(29247..30749)
                     /locus_tag="LTU54_001058"
     CDS             complement(29247..30749)
                     /locus_tag="LTU54_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL8295785.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            31008..33002
                     /gene="uvrB"
                     /locus_tag="LTU54_001059"
     CDS             31008..33002
                     /gene="uvrB"
                     /locus_tag="LTU54_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL8295786.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            33012..35828
                     /gene="uvrA"
                     /locus_tag="LTU54_001060"
     CDS             33012..35828
                     /gene="uvrA"
                     /locus_tag="LTU54_001060"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL8295787.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            36091..37356
                     /locus_tag="LTU54_001061"
     CDS             36091..37356
                     /locus_tag="LTU54_001061"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HBL8295788.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            37360..38091
                     /locus_tag="LTU54_001062"
     CDS             37360..38091
                     /locus_tag="LTU54_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HBL8295789.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            38226..39110
                     /gene="rapZ"
                     /locus_tag="LTU54_001063"
     CDS             38226..39110
                     /gene="rapZ"
                     /locus_tag="LTU54_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBL8295790.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            39107..40105
                     /locus_tag="LTU54_001064"
     CDS             39107..40105
                     /locus_tag="LTU54_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBL8295791.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            40139..41074
                     /gene="whiA"
                     /locus_tag="LTU54_001065"
     CDS             40139..41074
                     /gene="whiA"
                     /locus_tag="LTU54_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBL8295792.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            41617..42267
                     /locus_tag="LTU54_001066"
     CDS             41617..42267
                     /locus_tag="LTU54_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295793.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            42454..>43016
                     /locus_tag="LTU54_001067"
     CDS             42454..>43016
                     /locus_tag="LTU54_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HBL8295794.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        14200 a         7484 c         8810 g        12522 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttctactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaacagg gattactaaa aaacatttct atgatcgcta tcaaaaaaca
    13801 attgaatcgg tgtacggtga tgtgctaagg gaattagagc aagaagggtt acttgttaat
    13861 gaagaagacc gtgtttattt gacaccaaga gggacctttt tggggaacaa tgtgtttgag
    13921 cgctttttgt ttgaaaaata gtttaaagaa tatttgtttt ttatctgaat ttaaataaaa
    13981 aaagttcaaa aatacaataa aggactattg aaacgttgat ataactggat ttacatcaat
    14041 tatttagaat tagcactctt gtgtttagag tgctaaaaaa tggagtcttt ctcttgacag
    14101 gagaagggct tcttgctata tttataagtg gattagcact tagaatatat gagtgctaat
    14161 gagaggtgag gaagatgtta acaaagcgac aaaacgacat tttacgtctg atcatccaac
    14221 actatacgag tagtggcgtg cccgttggtt ccaaaacatt gatgtctgag ggagtcaagg
    14281 ccagctcagc aacgattcgc aatgatatga aagcattaga agatgaagga ttgctgttga
    14341 agactcactc ttcttctggg cggattccat ctgctttagg ctatcgttat tacgtggatc
    14401 atttgttaaa acctgctcgc gtagcagaag atgatttgct gctgattcgt cagtctctag
    14461 gcagagaatt tcatgaaatc aatgagatta ttaagcaatc tgctgagata ttatctgagc
    14521 tgacaagcta tacgactttt tcattgggac ctgaaataaa agaccgccga ctgactggct
    14581 ttcgtatcgt acccttgaat aatcgacaga ttattgctat cgtcgtaaca gatatgggga
    14641 atgtagaaag ccaagtgttc actcttcccg aatatatggg aagtcaggaa ctagaaaaaa
    14701 tggtacagat tgtaaacgac cgtctagtcg gagatcctct gatgacggtg taccataaac
    14761 ttcgtacgga gattccgatg attcttcata aatatttcca aacgacagaa gggatgtctc
    14821 agttatttga caccgttctt ggtcatgttt ttgaagataa ggtttatgtc agtggacaaa
    14881 tgaatctctt ggattatgag ccccgtcaag atcttgatca gttcaaatcg atgttttctt
    14941 ttatgaaaga ctcagacgga cttacacaaa tgatcactcc gatggatagc aatattcata
    15001 tcaagattgg tgctgaatta ggcaatgatt tattgcagaa tatgagtatg attcaagcaa
    15061 gttacgagat agatggtcat ggaagaggta caatagcctt gttagggcct actagtatgc
    15121 cttattctcg gatgttcggt ctagtcgatg tttatcgaag agaactagca gctaaacttg
    15181 ctgattacta ccgttcgctt gacttgtcaa actcgtaatg tgaataacga agctatttgc
    15241 agctgatcgt tctctctttc ttagtaagag caatcaaaca gctgactgaa taatgaaagg
    15301 aagactgtat cgtgaaagaa aatcaagaag aactagataa agaaatgaca gatgctcagc
    15361 cagaaccgga aatcgatgtc gaagctgcag aagattcggg tatttcagaa gctgaagcgg
    15421 aagaattcga aacagcgaaa ttaaaagctg agttagaaga aatggaagat aaatacttgc
    15481 gggctcgtgc tgaaatcgca aatatggcaa atcgaggaaa aaatgagcgt gagcaattac
    15541 aaaaataccg ttcacaagat ttagccaaaa aattacttcc atcaattgac aacctggaac
    15601 gtgcattagc cacagaagtg tcagatgatc aaggggcagg tttgaaaaaa ggcgtggaaa
    15661 tggtcttgga aagtttgcgt aacgcactgg aagaagaagg aattgaaaaa atccctgcta
    15721 aaggagaagc ctttgatcct aatcttcatc aagcagtaca aactgttcca gcgactgaag
    15781 atacaccagc cgatacgatt gtagaagtac tacaagaagg ctacaaatta catgatcgcg
    15841 tgttaagacc aaccatggtc attgttgcac aataattgtg cggatataaa acttacactt
    15901 tcaattaaag aaggagacgt taattatgag taaaattatc ggtattgact taggtacgac
    15961 taactctgca gtagcagtat tggaaggaaa cgaagcaaaa atcatcgcta acccagaagg
    16021 aaaccgtaca acaccttctg ttgtatcatt caaaaatgga gaaatccaag taggggaagt
    16081 tgcaaaacgt caagctgtga caaacccaaa tacgatttca tcaatcaaac gccatatcgg
    16141 tgaagcaggt tacaaagtag atgtagaagg taaatcatat acaccacaag aaatctcagc
    16201 aatgatcctt caatatatca aaggattcgc agaagactat ttaggtgaaa aagtcgaaaa
    16261 agcagttatc actgtgccag cttactttaa tgatgcacaa cgtcaagcaa caaaagatgc
    16321 tggtaagatc gctggtctag aagtagaacg tatcgtcaat gaaccaactg cagctgctct
    16381 tgcctatggt ttagataaaa cagatcgcga cgaaaaaatc ttagtattcg accttggtgg
    16441 cggtactttt gacgtatcta tccttgaact aggagacggt gtctttgatg tattgtcaac
    16501 agctggcgac aaccatctag gtggggacga ctttgataac aaaatcatcg actacatggt
    16561 agcagaattc aaaaaagaaa acagcattga cttgtctcaa gacaaaatgg ctttacaacg
    16621 cttgaaagac gctgctgaaa aagcgaaaaa agatttatct ggcgtatcaa gcacacaaat
    16681 cagcttgcca tttatcacag caggagaatc tggaccattg catttggaaa tgacattgac
    16741 acgtgcaaaa tttgatgaat taacagctga cttagtagaa cgtacaaaag taccagtacg
    16801 tcaagcgcta aaagatgcag gtttatcaca atcagaaatc gacgaagtta ttttagtcgg
    16861 tggatctact cgtatccctg cagttgtaga agcagtacgt aaagaaacag gaaaagaacc
    16921 aaacaaatca gttaaccctg atgaagtagt tgctatgggt gctgctatcc aaggtggcgt
    16981 gatcactggt gatgtgaaag atgttgtttt acttgacgtt acaccattgt cattaggtat
    17041 cgaaacaatg ggtggagtgt tcactaaatt gattgaccgc aatacaacga ttccaacaag
    17101 taaatcacaa gtattctcaa ctgcggcaga taatcaacca gcagtagaca tccatgtatt
    17161 acaaggtgaa cgcccaatgg ctgctgacaa caaaactcta ggaagattcc aattgacaga
    17221 tattccagca gctcctcgtg gtattccaca aatcgaagta acattcgata ttgataaaaa
    17281 cgggattgta aatgtatctg cgaaagatct aggtacacaa aaagaacaaa aaatcacgat
    17341 caaatcttct tcaggtctaa cagacgaaga aatcgaacgt atggtaaaag atgcagaagc
    17401 aaatgcagaa gcagataaag ctcgtaaaga ggaagttgac ctacgtaatg atgtagatgc
    17461 attgctattc tctgtggaca aaacattgaa agaattagaa ggcaaagtgg acgaagaaga
    17521 agtgaaaaaa gcagaaacag ctcgcgatga attgaaagcc gctgttgaag caaacaacat
    17581 cgaagaaatg aaaactaaac gcgatgcatt gaacgaaatc gttcaaaact tgactgttaa
    17641 actatacgaa caagcggcac aacaacaagc tcaagaaaac ccagaagcag ctcaaggcgg
    17701 agcagatgat gtcgttgatg ctgattttga agaagtcgat ggcgacgata aataatcagt
    17761 tcactgaatt tcgagagaag gccaaagttg ctactttggc tttttctttt gaaaaatagt
    17821 ttgaagtatc ggtttttgtt tcaaacaaaa tatgatatga taacaagcga cgtgaaaatg
    17881 atgagaacca ataggaggaa agcctatggc aacaaaacgt gattattatg aagtcttagg
    17941 gctctcaaaa ggagcatcag aagatgaaat aaagaaagct tatcgtaagc tttcgaaaaa
    18001 ataccatcct gacatcaata aagaacctga tgcggaagaa aaattcaaag aagtatcaga
    18061 agcatatgag attttaagtg acccgcaaaa acgcgctgca tatgaccaat acgggcatgc
    18121 aggcacagat ccaaattacg gtgctggtgg agctggtggc ggatttggtg gattcggcgg
    18181 cttctctggt ggcggcttcg gtggttttga agatatcttc gactctttct ttggaggagg
    18241 cggtcggtca gtcgatccaa atgcgcctcg tcaaggtgct gatttgcaat atacggtcga
    18301 cttgtctttt gaagaagcga tttttggtgt agaaaaagaa atcaagtata atcgggaaga
    18361 aatctgtcat acttgtggtg ggaacggtgc aaaaccaggc acacaaccaa ctacttgtca
    18421 taaatgtcat ggtgcaggta cgattaatgt agaacgccaa acaccacttg gacgagtgat
    18481 gagccgtcaa acttgtgatg tttgtcatgg gacagggaaa gaaatcaaag aaccttgtcc
    18541 aacctgtcat ggctcaggac atgagaagaa agcacattca gttaaagtga atgtaccagc
    18601 tggtgtagaa gaaggacaac aaatgcgttt agctggtcaa ggagaagctg gagaaaacgg
    18661 cggaccattt ggtgatttgt atgtggtctt ccgtgtagaa gaaagtgata tctttgatcg
    18721 tgaaggttct gaaatttact atgaattgcc tttaagtttt gtacaagcag ctttggggga
    18781 tgaagtccaa gtaccaactg tccatggaga tgtgaaattg aagatccctg ctggaacaca
    18841 gacaggtacg aacttccgtt tacgtggaaa aggcgctccg aagttacgtg gtggtgcaac
    18901 aggcgaccaa catgtcaaag taaaactgat cacaccgaaa aatctaaatg aacaacaaag
    18961 agaagcatta cgtgcattcg ctgaagctgg cggcatcaaa gttgaagaac agcaagaaga
    19021 cggtttcttc gataagttta aagatgcttt tggcgggaaa aagcggaagt agtttttcta
    19081 gtaaaaacgc taagtcatta ataattaaaa accagacgaa caatcatatg ttcgcctggt
    19141 ttttttgtag ttgtcatgtt tgtaccaaca aaaaagccag ctttagaata tgataatcgt
    19201 catagtttct aggggtataa ccagtgagag aatggaactt gttcaatcga tattgcaagg
    19261 tgtttttatg aataaataat tcttctgagc aatttttgat gcttccgtta tttttactga
    19321 agcatgccaa agtcttcttg aacaaatgta cttcttcttc tgtcaggctt ttcaaaatac
    19381 gttttacata taatgttcgt tgatctgacg ggattgctgg taaaacgatc ccgaggtcta
    19441 aatcttcaaa aaagcggatc tcgttcacag attgatggaa agctatccat tcgcaaactt
    19501 gtttggcatg aaggaaagat tgcggtgtct cttgctctgt ataacaaagc gacccaattc
    19561 caaagtttag ctgtaactga tacattttat taaactttgt ctggatttta tcaagcaatg
    19621 atagcaatga ggctctggaa atccgattga ctaggataat gatttgttgt ctatgtacag
    19681 tgagcaaagc ctgtccgtaa taagagaaga ccctttcgag caactccaat aaaggattga
    19741 tccgttcttg acacaattgc tgattgtcag aaagttgtcc cacaactaca taatatggag
    19801 aagaaaaatc aagctcaatt aagtgagggt ggtatcgact atgtacttga tatcatcaat
    19861 gaaccgatgg tactttctta tgcgatggaa caggtagagc gattgcttgt ttgtgcagga
    19921 aaacaagcga tgcggctgat acgattaggt gagaaaataa gaagataaaa atacacggtc
    19981 ccttcatggc gaccgtgcat ttttggtttt tattaaattt tatatctaat tttcaattat
    20041 ccgcataaac actatacatt caagcatttt gaagtataga aaaacactca tcttaccacg
    20101 aatttaccac gattgaatga gccttgatat gaccgtaaaa ctactaaggg agataacacg
    20161 aaactgtgta tctcccgatt tttttataca gttgattact tcactccgat ttcaaatttc
    20221 aatattgctt tcatcatgtc tgtatgaatt tctcctttta gttcttcatc aacctgcata
    20281 atgattttac cactttcaga gtggtaaaaa ggacgcaagc ataatttaga aatataaccg
    20341 tcataaaact tcaaaatctc tttaactgcc atttcatctc cgcttgacgc tttgcaaatc
    20401 aattcaaaag agggatagcc ataatcttta cttcctttcg ctcaaaagca tttttaactt
    20461 ccttagtcct ctagttcttc tactgcaaat agcagaacgt gatacatggt acagttctga
    20521 aatttctctg tcgttcattc cttgaaaata acgtaataaa atcgcactac gctgtttttc
    20581 tgatagtcta tgtaaaactg taccaagtgt ttcatcagat acataaattg taaagtgcaa
    20641 gactttaaat gatacacaat cataaaaaca ggtatcaatc gttccacgtt caacttgttt
    20701 cgtttcggct atttcacaaa acaatacttc acgtttagaa tgtcttgcaa gctgtctgtt
    20761 tcgacttttc actttgttct taatgacaat cagcattaaa gaattgaact gtaatctgac
    20821 gatatgctcg aaagaagatg tcctcataat ctcaccccct ttctgtctag gaaaggaagt
    20881 gagccttgct cgtttatctc ctttcactct tagtcccaat gtgaaagggg gatttgttgc
    20941 attactgata aataaacttt gtaaaaaagt tctgaatagc caaaaaagca tataaacaga
    21001 tttatttctc tgtttacatg cttctgttat tctatctata tgatttataa aaccacattg
    21061 gtggacgtac ttatctattg cagatagacg actttttttg acaagaaccc aatgtaagga
    21121 aatttattgt atatgatgta cttcatggcg acgttgacct ccaacaaacc gccatttgga
    21181 agtaatatac aatattttaa cagcgtaaat agcactacca tataacggtt ttttttattg
    21241 gcgtttagta gtgcttttta ttaaatataa acctataaac catataacac gtttttctat
    21301 acctgttttt aattcagtag gaacaataaa atgtatagag gtggtctact atgcgtaaaa
    21361 aagaagataa atatgatttt agagcctttg gtttagccat taaagaagct cgattgaaac
    21421 gaggtttaac tcgtgaacaa gtgggagcat tgattgaaat tgacccacgg tacttaacta
    21481 atattgaaaa taaagggcaa caccccagca tacaagttct ttatgacctt gtatcgttac
    21541 ttcatgtttc cgttgatgaa tttttcttac ctgctaataa cttggtaaaa agcacccgac
    21601 gattacagat agagaaatac atggatagct ttacagacaa agaactatcc ttaatggaat
    21661 ctttagccag cggtatcaac gaagcaagaa acatcgaaga ctaattaaaa gaatccatac
    21721 ataacggaaa gagccgataa aatgagattg tattaatctc attttatcgg ctctgcgtct
    21781 ttgcgtctgg ctctgtaatc acagttactt tgaactgctt tatttcaatt aaattttctt
    21841 gtctgcattt cggacaatag agggggaatt tttttaattc agtatcttcc cttatcttta
    21901 atcgtgtttt atttccacat acaggacaca atatccactt gtagtttata ataactatct
    21961 cctcctttac actttaattc aaatctttat taaaaaatat ttcatcttat ttaacaagaa
    22021 accatattta tataacaaca taaaatacac taagttattt tattgaacat atatcgtact
    22081 ttatctatcc gactatttgg acgacggggc tggcaaacag gttcaccggt agtaacatgg
    22141 taccctttta actctgttaa acaaacacta cgtccatttg taaagaaagt taaatcacta
    22201 cgatattctt gaatacaccg agcagggatt tctccactaa gaatgacctc attatttttc
    22261 aattgagtgt ctacgatgtt cgcacaatat ttaggagcat cgttgtatgc tcgtgaaaga
    22321 tattcctgtg gcgcataaat tttaaaacta agatatggct ctaacaattc tgttccagct
    22381 ttttttaaga cttgttccaa tacaatagga gcaagcatcc gaaaatctgc tggggtacta
    22441 acagggctat agtataagcc atacttaaaa cagattttac agtccgtcac attccaacca
    22501 tacaatcctt gttcacagcc atagcgtatc ccctccataa ctgcattttg aaacgattga
    22561 tttaagtatc caagagaaac cgagctctca tactgcattc cacttcccaa cggaagcggt
    22621 gatacagata aaccaatgga agcccagaaa ggatttggcg gcacttcgat gtgaatggta
    22681 tattctgcat tttttaacgg tctctccata taaatgactg taggctcttt tagttctatc
    22741 tccacatgat acttttcttg caacagtgca ctaatcactt ccatttgtac tttccctaag
    22801 aaagaaagta taatttcatg tgtcgtagaa tccacgtaat atcgtagaag cggatcacta
    22861 tctgagattt ccaaaagggc atcaagcaac atttctctct gttcaggttt actcggttca
    22921 acagttgttt gtagtagagg gtgcggattt tcaatctttt ttctctgtgg caatagtttt
    22981 gtatctccaa gaacactatt taacttcaaa aactcatttt gcaaaataac aatttctcca
    23041 gaataagctc tatcaatctt acataattca ccatttattg aagtatacat ttctgtaact
    23101 tttatttttt ctttttctga tactctaacc gaatctcgta aatgtagtac tccactataa
    23161 aggcgtatat atgcaagacg ttgtcttttt tttgtatatt caattttgaa aacatttccg
    23221 caaagttcag acggacctcg atgtgttgat gaataaaatt tattagtaat aacttctata
    23281 aggttatcaa tccctatatt actttttgca cttccatgat aaagagggaa cagagaacaa
    23341 ttctgaaatc ttatgctttc ctcttgttcg agttccaatg cttctaatga tttaccggac
    23401 atatatttct ctaaaaggtc atcgtttccc tctattaccg tatcccattg ttcagattcg
    23461 gtaaagttcg tcacacacat attaggatac agttctacct tctgtttgat tacaatttcg
    23521 gcagaaagtt tctctttaat atcctgataa accgttgata aatcaattcc attttggtca
    23581 atcttattga taaaaaagat tgtgggaatc cccattttcc taagtgcatg aaataatata
    23641 cgagtttgtg cttgtacgcc atcttttgca gaaatcagta gaattgcccc atctaaaact
    23701 gataatgaac gatatacttc tgctaagaaa tccatatgtc ctggcgtgtc tatgatgttc
    23761 accttcgtat tttcccactg aaaagaggtt attcctgtct gaattgtaat tcctctctga
    23821 cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt ccacgcttcc taattctgta
    23881 atcgctccac tgttatataa taagctttct gttaaggtag tttttcctgc atcaacatga
    23941 gctaaaactc caatattaat aattttcatg tgattttcct ccattcaaaa acccaaaagg
    24001 gcataaaaat cccagtgata aatactttta tcactgggat ttttatgcat aaccataggc
    24061 atacaaagca tacagatatt ctccggatac tttagaatca catgataaag gtattcttaa
    24121 actgggtaca aaaaactaag ccctcctaaa aaaggacatc caattatttg ttcccactat
    24181 caaattgaca gtttatttaa gaataccttg ccgcatattt attaactcct tttaaataga
    24241 tacttaaata atagcacgta agagcatatt tgtaaaggaa tctccaattt tttatcaaag
    24301 agagtacgtg attacaaaat agctgtaata atgtaccaat atttgttatt ctataatctt
    24361 ccaattactc ccgttctttt caagtaccaa atcaaattga gatacctgcg ttgcctttgc
    24421 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
    24481 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
    24541 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
    24601 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
    24661 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
    24721 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
    24781 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
    24841 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
    24901 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
    24961 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
    25021 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
    25081 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
    25141 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
    25201 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
    25261 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
    25321 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
    25381 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
    25441 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
    25501 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
    25561 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata caatcccaaa
    25621 gttgtgagta gtaatgagaa gttttaagaa gatagtgcat atcacacttt ttgaatagag
    25681 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
    25741 tgcacttgaa atgaagaaca aacgtcttgc aagcggtctt tcacaaggag aacttgcaag
    25801 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ccaacgccag
    25861 caaggaaaca caaaatatta tcctgcgttg tctgaattat tatatctgcg ataagccttt
    25921 ttatctgctc gttgattatc tgtctgtccg ttatccaacg actgacgcat tggaaattat
    25981 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
    26041 ctataaagag cattacgcct acggagaaat caaggtcatg gcttctgatg acgaacacat
    26101 gggcgtattc ttggaattaa aaggggcagg ctcacgcaac atggaatatg tcttgcaggc
    26161 acagaacaga gattggtatt catttttaaa ccgctgtctt gactgtgacg gtgttatccg
    26221 acgttttgac ttggcaatca atgatatgtg tggtctgttg gatattcccg ttttgtctga
    26281 aaaatacaga aatggcggtg cggattgtcg ctgtaaaaat tatgaaaatg tgcagggtgg
    26341 aaaactaagc ggaaaaaacc gtaatttagc aagcaccctt tatatcggtt caaaggcaag
    26401 cacaaaatac ttctgcctgt atgaaaaaca aaaggaacag gcaaccaaaa agaaacatac
    26461 ggatattatc aatcgttttg aaattcgtct gcgtggtaca aaagcagtac aggcagttga
    26521 ggaattgtta ttgacatata accctcatgg gctggtgttt taccttatta ctgattttgt
    26581 gcaatttccc gactatccgc tgtgggagat attcatttcc catgacagtt taccttttga
    26641 aatgaacccc gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    26701 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggctcaaact acatgaaaat
    26761 gattgatgaa tgtacccacc tctctgaaaa gcaggaaatg cttgtggaac agatgtgtac
    26821 ggacatagca gacgtaatcg aaagtgaggg ggtgttttat gagtaatgca caggatattc
    26881 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgtatcggag
    26941 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    27001 gcaaccgcat acagattaaa cgccgacagt ttgaacaggt cattgataaa ttggacgcta
    27061 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    27121 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    27181 tgcatactgg agagagccaa cgaacagaca gaaaatactt atataaatat gtagacgcat
    27241 ttggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    27301 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    27361 acggtatcga cagcactggc aagaaaatga cgctttgcca actttatgcc aaacagaacg
    27421 cacagagggc aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    27481 tgaaagagga caagttaggt gctaggagca ttgatacaat caaaccctct gacgctaagg
    27541 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    27601 gctcgttaaa agcgtcattc tacatagcca ttcaagatga ttatgtgagg aaaaatccct
    27661 ttgatttcaa gttgagtgaa gtcctagaaa atgataccaa aaagaaagtc gcattgacag
    27721 aggaacagga acaagcctta ctctcattca tcaagacgga caatgtgtat cacaagcatt
    27781 atgatgatgt gctgatactg ttaaagacag gacttcgtat ttcggaactg tgcggactga
    27841 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    27901 gcaaggaaca gggctattat attgaaacgc ctaaaacaaa aagcggaata aggcaagtac
    27961 cattaagcaa agaaacgata caggcatttc aacaggttat gaaaaaacgt ccaaaggcag
    28021 aaccatttgt gatagacgga cggggcaact tcctatttgt caatcataag ggcaagccca
    28081 aagttgcgat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    28141 acaaggacaa tccattgcca catatcacac cgcatacgct acgccataca ttctgcacaa
    28201 gactggcaag caagaacatg aacccaaaag atttacagta tatcatgggg cattcaaata
    28261 tcagtatcac aatgaactgg tatgctcatg cgtccataga taccgcaaaa tcagaggttc
    28321 agcgtctaat tgcatagaag tatttaccac gattttaacc acgcttgata gtgaaaatat
    28381 aagaagatag acctagatat gtgaggttta ccacaaaagc aaaatgcccg tagagcctat
    28441 aaaataaggc tttgcggaca tttaagaaga tataaaaaga tagtcaaaaa gacatatata
    28501 atttatttta aatatttaat actttattca agaaatcttt tgtccgttca ttttgcggat
    28561 tttcaaagat ttgttgaggc gttccatctt ctaaaaagtt tccgtcgtca atgaacatga
    28621 cgcgattggc cacttcttta gcgaacccca tttcgtgcgt aacgataacc atcgtcatac
    28681 cttgttctgc taactttttc atgacgttca agacatcacc gaccatttca ggatcgagtg
    28741 cagaagtagg ctcatcaaaa agcatgatgt cagggttcat cgctagagca cgagcaatag
    28801 ccacacgctg tttttgtcca ccagaaagca tttcaggata catatctttt ttatcttcta
    28861 gaccaactgt ctgcagtaat ttgattgctt ttttctctgc ttcttctcta gattctttct
    28921 tcaggtccat cggtgctaaa atgatgtttt ccaaaataga aagatgaggg aataggttaa
    28981 agtgttggaa taccattccg atatgttgtc tcaccaagtt gatatctgtg cttttatcca
    29041 tcaagtgggc gccgtcaata ataatttcac cgtcagaagg ttcttctaaa cgattcaagc
    29101 aacgcaagaa tgtacttttt cctgaacctg aagggccaat gatacagacg acatctcctt
    29161 cgttgatgga tacattgatg tcgtttaata ctgtgttatc gccgtatttt ttgactaaat
    29221 gctcaactaa gattttttct gccatcttac ttcaccttct tttctaaaac ttttgctaat
    29281 tttgtcaatg ctgtgatcaa aatcaagtac atgatcgcaa tgatcaagta aacatacgta
    29341 ctttgtgttg tacgtgcaac aatgatctta ccagtttgca gcaattcgac tacaccgatt
    29401 gcggaaatga tcgttgtgtc ttttaatgag ataacgaact gattgacgaa tgatgggatc
    29461 atgatgcgta tcgcttgcgg caaaatgatt ttttgcattg tccgattata agaaagacct
    29521 aaacttctgg aagcttccat ttgtccgaca ggaacggcgt tgatcccgcc tcgaacaatc
    29581 tctgcgatat aagcacttgc gttaagagtc aaggtgataa caccagcaag gaagtcagga
    29641 atcgtaaatc caacgatacc aggcaaccca aagaaaataa agaacgcaag aaccatcatt
    29701 ggaattcctc ggatgatatc gacataaata ctagcaattg tgcgaagagc gcgtattggt
    29761 gctacactga ataatccaaa aatcacaccg attactatag ccaaagcaaa ggagatcaaa
    29821 gttaggagaa ttgttctcca aagtcctttc aacaatgctt gatagttgtt ttggataagt
    29881 ccaagaaatg ttgattcatc agcagtggaa gactcactgg aagtttctga accatccgct
    29941 acatattctt taataatctt atcgtattcg cctgttcttt ttaattcttt caatccttca
    30001 ttgaacatat taagaagctc aggattttgt ccttttttaa cggcaaatgc atattttccg
    30061 ccagattctt ttttgatcgg tgtttccaat tcttgtcctt gtttgatcgc ataaccgatt
    30121 accggataat catccatcat tgcatcgatt gcgccaactt ttaacccgtc gtacaattga
    30181 tcagcagcat caaaatattt gatagaatag ccatattcag attcatggtc gcttaaaaat
    30241 tcagcacttt cggtaccaac ttttgctccg actgtttttc ctttcaaatc ttcataagac
    30301 ttgatcgaag tatctccttt tttgacagct aattggatcc cgctatcaaa gtaagaatca
    30361 gaaaaatcat agcttttctt tcgttcatct gttacagtca ttccggcaat cattccgtct
    30421 gcttgtcctg attccaaagc ttgcaaagca gcagagaaac cacggtagtc cattcggatg
    30481 ttaaatcctt gcagtttggc gatgttttcc atcaatttta catcgatgcc ttcgtactcg
    30541 ccctttgcgt tttgaaattc aaatggagca aaagcagagt cactggcaat tacgtatacg
    30601 tcttttttgg gctctatttt ttgcattgtc tcgcttgtgc ttgactctgc cgttgtagtg
    30661 ttgtcagctt gtgcaacatg accaaacgca agtgtcacaa gtcctgtcat taagaaaaca
    30721 aatgagtgaa ataaatgctt tcttttcata atcaacctcc taaatttcca atattttatt
    30781 attataggcg aaaagtgctg aatcagacaa gactgtaagc tcgaaaaaat gaaaaaaatg
    30841 cgagtttcta acattttatg ttataaaaca taacatgtat aataagataa aaaagtggcg
    30901 aaagtatgtt cggtgtggta taatttttta gtaaggaatg cctttgcttt ccttttcttt
    30961 ttattggtcg taaaatgaaa aggaatgaat gaaaaggagg acgaagaatg attgagagag
    31021 atacctcccg acactttgag cttgtctcga aataccaacc ggctggtgat cagcctgaag
    31081 cgattaacca attagtggat ggcgtagtcg gcgggaaaaa agcacaaatc ttattaggtg
    31141 cgactggaac agggaaaaca tatacgattt ccaatcttat tgagaaagtg aataaaccaa
    31201 cactgattat tgcccataac aaaacattgg ctggtcagct gtacggggaa ttcaaggaat
    31261 ttttccctaa caatgctgtt gaatattttg tttcttatta tgattattat cagcctgaag
    31321 cttatgtgcc ttcaagcgat acgtatatcg aaaaagattc aagtgtaaat gatgagatcg
    31381 acaaattacg acattcggct actagttcat tattagaaag aaatgatgtg atcgtcattg
    31441 catccgtttc atgtattttt ggattaggct cgccttttga gtaccaaaaa caagtagttt
    31501 cgattcgaca gggagcagaa ctagatcgca atcagttgat tcgtgactta gtcagtatcc
    31561 aattcgaaag aaatgatata gattttcagc gcggtcgttt tcgagtgcgc ggtgatgtcg
    31621 tggagatttt tccggcttca agagatgaac gtgccttacg tgtggagttt tttggagatg
    31681 aggtcgaacg tatccgagaa gtcaatgcac tgacaggtga agttttagga gaaacggaac
    31741 acgtagcaat cttcccggcg actcactttg tgacgaatga tgaacatatg gagcatgccg
    31801 tagcgaatat caaagaggaa ttagaacagc gtttaactgt cttgcgaaat gaaaacaaac
    31861 ttctggaagc tcaacgttta gaacagcgaa ccaattacga tattgaaatg atgttagaaa
    31921 tgggctacac gtcgggaatt gaaaattatt ctcggcatat ggacggacgt aaagaaggtg
    31981 aaccgcctta tacgctattg gatttctttc ctgaagattt tttgatcgtg gcggacgaat
    32041 cgcacgtaac catgcctcaa atcagaggaa tgtataatgg tgaccgagcg agaaaacaga
    32101 tgctagtaga ttacggtttc cgtttgccat ctgctttaga caatcggcca cttcggttag
    32161 aagagtttga aaaacatgtc aatcagatca tttatgtctc tgcgacgcca ggaccatatg
    32221 aacatgaaca aacagatacc gttatccaac aaatcatccg tccgacaggg ctgcttgacc
    32281 cagtgatcga agttcgtcca atcatgggac aaatcgatga tctagtaggc gaaataaatg
    32341 aacgagtcga aaaagaccag cgggtgtttg taacgacatt gacgaaaaaa atggcagaag
    32401 acctcacaga ttatttcaaa gaattaggga tcaaagtcaa atatttgcat agtgatatca
    32461 aaacgctgga acgaacagaa atcattcgag atcttcgatt aggtgaattt gatgtgttag
    32521 ttggaatcaa tttgcttcgt gaaggattag acgtacctga agtttccctt gttgctatct
    32581 tggatgcgga taaagaaggc tttttacgta gtgaacgttc cttagtccaa acgattggac
    32641 gagctgctcg gaatgaagaa ggtaaagtaa tcatgtacgc agacaaagta acagattcta
    32701 tgcgtctagc gatggatgaa acgtcaagac gtcgtacgat tcagcagaag tacaatgaag
    32761 aacatgggat cgttccaaaa acgatcatta aagaaatccg agatttgatt tctattacga
    32821 aagaatcgga agatgataca aaagaagcgg tacaagtctc gtacgaagaa atgacgaagg
    32881 aagaaaaaga cacgttattg atgaaattag aaaaagaaat gaaagatgcg gcaaaagctt
    32941 tggattttga aacagccgcg aatgtgcgag atatgatttt agaattaaaa gcttcaaagt
    33001 aggaggacaa catggctaac gataaaatta cgattcatgg tgcacgtgcc cataacttga
    33061 aaaatataga tgtaacgatt ccacgagata aattcgtggt cgttacaggt ttgtccggtt
    33121 ctggaaaaag ttccctagca tttgatacgc tgtacgctga agggcagcgg agatacgtag
    33181 agagcctttc tgcttatgct cgtcagtttt taggacaaat ggataagccg gacgtagata
    33241 gcatcgacgg attgagtccg gcgatttcta ttgatcagaa aacaacaagt aaaaatcctc
    33301 ggtcgacagt aggtactgtg acggaaatca atgactattt acgcttgttg tttgcacgtg
    33361 taggacatcc gatttgtccc aacgatcata tcgagattac cagtcaatca gtagatcaaa
    33421 tggcagatca agtaatggct ttgcctgagc gcacaaagat ccaagtgttg gcaccgatcg
    33481 tggtaaagaa aaaaggacaa cataaaaaaa tattcgaacg aatccaaaaa gagggatacg
    33541 tccgcgttcg agtcgatggt gagatctatg atttaagtga agtgcctgag ctggagaaaa
    33601 acaaaaaaca tgatatcgcg atcgtcatcg accggatcat tgtgaaagaa gggattcgtt
    33661 ctcgcctatt tgattcttta gaggcggccc tgcgattagc agacggctat gctctggttg
    33721 acgtgatcgg tgaagaagaa atgttgttta gcgaacacta tgcctgtcct tattgcggat
    33781 ttactgttgg cgaactagaa ccaagactgt tctcattcaa tgcgcctttt ggagcttgtc
    33841 cagactgtga tggtttaggt gtcaaattag aagtcgatca agatttagtg attccagata
    33901 gaacgaagac acttagtcaa ggtgctatcg ttccatggaa tccaatcagc tctcagtatt
    33961 atcctcaaat gttagcgcag gcttgtgaaa gtttcggtat cgatttagat acaccattcg
    34021 aagaattacc ggaagagcat caggagatca tcttgaatgg gacagatgat cttttccatt
    34081 tccattatga aaatgatttt ggtggcgttc gcgatgtaga aacgactttc gaaggggtat
    34141 tgaagaatat cgagcgtcgt tatcatgata cgaacagtga tttcacgcgt gagcaaatgc
    34201 gtctttatat gacagaatta acttgtcaga catgtaaagg ttatcgattg aatccccaag
    34261 ctctatctgt tcaaatcaat ggaacgaata tcgggcaaac aaatgagttg tctattcaaa
    34321 aagcagtaga tttcttctca aacttaacgt tatctgaaca agagaaagta attgccaaac
    34381 cgattttaaa agaagtaaat gacagacttt cattcttgga aaatgtggga ctagactatc
    34441 taactttgag tcgcgcttcc ggaacattat ctgggggaga agcgcagcga atcagattgg
    34501 ctacacaaat tggatcgaat ctttctggag ttttgtatat tttagatgaa ccatcgatcg
    34561 gattgcatca acgcgacaac gatcgactga ttggctcatt aaagaaaatg cgtgatcttg
    34621 gcaacaccct cgttgtagtc gaacatgatg aagatacgat gtgggcagca gattatttga
    34681 ttgacgtcgg acctggggcc ggagaacaag gcggacaaat cgtggctgca ggcactcctg
    34741 aagaagtagc taatgatgaa aattctttga cagggaaata tctttcaggg aaaaaatcaa
    34801 tccctgtacc aaaagaacgc cgtaaaggta atgggaaagc aatcaagatc actggtgctt
    34861 cagaaaacaa tctgaaaaat attagtgttg aatttccatt aggagaattt gtagcagtca
    34921 caggtgtgtc tggttctgga aagagtacat tggtcaatag tatcttaaag aaatcgttag
    34981 cgcaaaaatt aaataagaat tctgctaagc caggtaaatt caagacaatt tccggctacg
    35041 aaagtatcga aaagatcatc gatatcgatc aaagcccaat cggccggacg ccgagaagta
    35101 atccagcgac ttatacaagt gtatttgatg atatccgtgg gttatttgct caaacgaacg
    35161 aggcaaaaat gcggggttat aagaaagggc gttttagttt caacgtaaaa ggcggtcgtt
    35221 gtgaagcttg tcgcggggat ggaattatta agatcgaaat gcactttttg cctgatgtct
    35281 atgttccttg tgaagtatgt catggtaaac gatataattc tgaaacatta gaagtgcatt
    35341 acaaaggaaa aagcattgct gatattttgg aaatgacagt agaggatgct gtagaattct
    35401 tcaagcacat tccaaagatt catcgcaaac tgcaaacgat tgttgatgtt ggcttaggtt
    35461 atgtgacgat ggggcaacca gcaacgacat tgtccggtgg tgaggcacaa cggatgaaac
    35521 ttgccagtga attgcacaaa atctctaatg gaaagaattt ctatatacta gatgaaccaa
    35581 cgacaggact tcatagcgat gacatcgccc gcttgttgca tgtattacaa agattagtag
    35641 atgctggtaa cacagtttta gtgattgaac acaatctaga tgtaatcaaa acagcagatt
    35701 atatcattga tttaggacca gaaggtggag aaggtggagg aacgatcctt gcgactggaa
    35761 caccagaaga aatcattaac gtaaaagaaa gttatacagg tcactatttg aaaaaaataa
    35821 tggtataaag aggatagata gctgagatag ttaaaaaaac ataaaaaaat gaatttttgc
    35881 ttgttttatt aatatcagtg ttctagaatc attacatggt agttctttct acactgattg
    35941 gtatagataa aaacttccta taagttcaat ccgttcaaaa aagaattgag caatcggagg
    36001 actttccttt gattgctttt ttctttatgg tatgatattc cttgtgtagt tggaaaagaa
    36061 aaacaagaga ttatgagtaa gtgagagata atgatgaaca gtattgaaaa tgaagaattt
    36121 atccctatat tattaggaag cgacatgaac gtttatggta tggcacgttc ttttaatgaa
    36181 gcatacggca aaatctgcca agcatatgct tcggatcagt tagcccctac ccgctacagt
    36241 aaaattgtca atgtagaagt tattccagga tttgataaag accctgtatt tatcgagacg
    36301 atgcttcgtt tggcaaaaga aagatattct gataagagta aaaaatatct tttgattgct
    36361 tgtggtgatg gatatgcaga attgatctcc caacataaac aagagctgtc agaatatttt
    36421 atctgtccat atattgatta ttctttattt gaaaggctga tcaataaagt aagcttttat
    36481 gaagtttgtg aagaatatga tcttccgtat cctaaaacat tgatcgtcag agaagaaatg
    36541 ttagtaaatg gacatttgga acaggaattg ccttttgaat tcccagttgc tttgaaacct
    36601 gcaaacagtg tggagtatct ttctgtccaa tttgaaggaa gaaaaaaagc ttttatttta
    36661 gagactagag aagagttcga tttaatttta ggccggatat acgaagccgg ttataaaagt
    36721 gaaatgattg tacaagattt tatcccgggt gatgatagta atatgcgtgt gctaaatgcg
    36781 tatgtagatg aagatcatca agttcggatg atgtgccttg gtcatccact attagaagat
    36841 cctacaccag catcgattgg taattacgtt gtgatcatgc cagattataa cgaaaaaatc
    36901 tatcaaacca ttaaggcatt tttggaaaaa atcgaatata caggctttgc caatttcgat
    36961 atgaagtatg atcctagaga tggagaatat aaattatttg aaatcaattt gagacaaggt
    37021 cgtagcagtt tctttgtgac attgaacggc ttgaatctgg ctcgttttgt cacagaagat
    37081 cgagtgttca acaaaccctt tgttgaaacg acctatggaa cgaatcaatc agataaagca
    37141 agattatgga tgggtgtgcc caaaaaaatc tttttagaat atgctcggga aaatgaagac
    37201 aaaaaactag ctgagcaaat gatcaaagaa aatcgttacg gtactactgt attttatgaa
    37261 aaagaccgat caatcaagcg ctggttgttg atgaaatata tgttccataa ctatattcca
    37321 agattcaaaa agtattttca tgtgaaagaa ggctagttca tggagaattt tttcagtatt
    37381 ttaggcggaa tgggcacgat ggcgacagaa agttttgttc gcttgatcaa ccaccgaacg
    37441 aaagctacaa aagatcaaga atatttgaat tatgtcttat tcaatcatgc aacagttcct
    37501 gatcgtacag cttatatttt agatcgatca gaagaaaatc caatgccgtt tctgctggat
    37561 gatattgaga aacaaaattt attgcgacca aacttcattg tgttgacttg taacaccgca
    37621 cactactttt ttgaagagtt acaagcggca acggatattc ctatcttgca tatgccaaga
    37681 gaagcagcaa atgaacttgt ccgtcagcat acgacaggga gagtagcgat tctaggaaca
    37741 gaaggaagta tgaaagctgg aatctatgaa cgcgaagtca aaaatcttgg tttcgaaaca
    37801 gtgattcctg atactgcgct tcaagaaaaa atcaattacc tgatctatca tgagatcaag
    37861 gaatcggatt atttgaatca agagctctat tacgaaatct tagaggaagc ggtagagcgc
    37921 ctaaactgtg aaaaagtcat cctaggttgc acagaattat ctttgatgca tgaatttgct
    37981 gaagataatc attacccagt gatcgatgct caatctattt tagcggatcg aacgatcgaa
    38041 cgtgcattag ctgaaagaag cgaggcattg gatacagcat cggaaaagta aaaaaattgg
    38101 atttggggca aatatcctaa aagatatttg gtccaaatct ttttgtaaaa gaaaaagaaa
    38161 actggtaatg atacgcccgg gatgatataa ttttattatg cacaatgtga gaggggttcg
    38221 taaatatgac agataatttg caattagtca tcataacagg tatgagtggt gcagggaaaa
    38281 cagtagctat ccaaagtttt gaagatatgg gctatttttg tatcgataat atgccgcctc
    38341 gattgattcc aaaattctgg gaattgatca aagaatcagg taaagtcaca aaaatggctt
    38401 taggagtgga tttgcgttct cgttccttct ttgaagaaat ccaaaacatg ctgatcgaaa
    38461 tcgaaaatac gaacttcatc gacacttcta tcttattttt agatgcttca gacgaagaac
    38521 tagtggctcg ctacaaagaa acaagacgag cacatccaat ggcgatggac ggtttgatca
    38581 ctgaagggat acgtaaagaa cgggcgatac tagacgactt gaaagcccaa gcttccgtga
    38641 tcattgatac gacaacattg acaccacgac aattacgtga aaaaatcaat gagtcattca
    38701 aatcaaaggg agaccaagga tttcggatcg aatttgtatc atttggtttt aaatacgggt
    38761 tgccgatcga ttcggatatt gtgatggatg tgcgtttttt gcccaaccct cattatatcc
    38821 cggaactaag accattgacc ggaaaagatc agccagtcta tgattatgtg atgtcctttg
    38881 atgaaacgca atcattctac cataaatttt tggatcttct ggaaactgta ttgcctggat
    38941 acattaaaga aggaaaaagc agcttgacag ttgcaatcgg ctgtacaggc ggtcaacacc
    39001 gttctgtggc attgactgaa cgtttggcaa atgctttagg gaaaaactat aaagtcaatg
    39061 tgacacatcg ggacaaggat aaaaggaaag agacggtgaa ccggtcgtga aaatgaaaac
    39121 ctaccggata agaaaaccaa agatcgtagt ggtcggcggt ggcactggtc tgccagttat
    39181 cctgaaaagt ttacgaaatc aaggagcaga tatcacagca gtagtgactg ttgcagatga
    39241 tggtggaagc agcggtgcaa tcagagaatc gatcgctatg gctcctcctg gtgacttgag
    39301 aaatgtctta gtggcgttat cggatatgcc acaattctat gaagatattt tccaataccg
    39361 tttcaaaaag gaagatcagt ttttagccaa tcatacgatc ggtaatctga tcattgccgc
    39421 agtttccgag atgcggggaa gcacttatga ggcgattcaa ttattagcaa aaatgatgca
    39481 tgtagatgga catgtttatc cttcttcaga aactcctttg accttgcatg ctgtttttaa
    39541 agacggaacg actgcagtag gagaatctaa aatcgcgatc aaccgtaaaa ccattgaccg
    39601 tgtgtatgtc tgcaacacaa atgataaaag tgaagccaaa gcggctcgaa aagtcgtttc
    39661 agcaattatg gacgccgaca tggtcgtttt gggacccgga agtttattca ccagtatcct
    39721 tccaaattta gtgataccag aaatcggcga agcaatgctc aagacaactg ctgaaaccgt
    39781 ctatatctgt aatatcatga cccaaaaagg ggaaacggaa catttcacag atgccgatca
    39841 tatacgtgtt ttacacaagc atttgaatca gccttttatc gatacagtat tggtcaatac
    39901 ggaaaaagtg cctgaagatt atatggaccc agagatctat gatgagtatc tagtacaagt
    39961 cgcacacgac ttcaacggat tgagaaatga aggatgtcga gtgatttcca ccgacttttt
    40021 agaattgaaa gatggtggtg tcttccacga tggagacaaa gtagtagaag aattgttccg
    40081 tttagttttc ggttcaaaat attaagcaat ttattgttgg agaaaggagg agaataggat
    40141 gtcatttgct gcagatgtga agaaagaact aactggtttg gaagttcatc gagaacatgc
    40201 gaaagcggaa ttagctgctt tgctgcgaat gaacggctct ttaggtattg tgaaccagca
    40261 gtttattttg aacgtccaaa cagaaaatgc tgcaattgcc agacgtatct attctctttt
    40321 gaaagatcat tatcaggtac gaagtgagct actcgttcgt aaaaaaatga aattaaaaaa
    40381 gaacaatgtc tatatcgtcc ggttgaagca aggcacaaaa gatgtgttga tggatcttga
    40441 tatcatggat ggtatgatgt tcaatggaca cgtttcggac gagattatgg gaaattcgca
    40501 aaaaatgcgt tcttacttac gtggtgcttt tatggcctca ggttcagtca ataatccgga
    40561 aacgagtcgt tatcatttgg aaatattttc tatttatgaa cagcataacc aagacatttg
    40621 cgatatgtta aattattacg gtctacatgc tcgaacgctt gaacggcgca atggctatat
    40681 ttcgtattta aaaggagcag aaaaaattgc tgatttctta acattgattg gtgcaaccaa
    40741 ctcaatgctc cgatttgaag atgttcgaat cgtacgggat atgagaaatt cggtcaatcg
    40801 tctagtcaat tgtgagacag caaatctgaa caagacgatt gatgcggctt ctaaacaaat
    40861 cgaaaacatc gaattgatcg aagcaaaggt cggtctacat gcccttcctg aaaaacttca
    40921 ggaaatcgca gaacttcggc ttcaacatcc agaggtcagc ttgaaagaat taggggaaat
    40981 gattccctca ggtgcaatat caaaatcggg aattaatcac cgaattcgca aaataaatga
    41041 atttgcggaa aaactgcgtg aagaagcagt ttaatccaaa gaaaaaactg atacatgaag
    41101 caggagatgc gaatgtatca gtttttttta aaagctatac ttgcttttct tccgtcaaac
    41161 tataattcca actttactgg agtaacataa tgttgaaagg cttctattgt tttttaatac
    41221 aatcgtataa aaagcgggga gacaactgaa tgaagatttt agttttatac ataaactttt
    41281 gcttaatata gaagtcctga tatagatagg atcaagtatt tatctataac tggagattaa
    41341 ttaaaaactg gaaggaactg ctcgatcaac cttgagaact gtttttctta cttctggtca
    41401 agattctcgt ggttggtgga taaggatggt tgataagttc attggattag ctagtcgaaa
    41461 acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt tattcagtga
    41521 taatcataag ttcataaata gtaattggga tgtatggttg gtatacgtta tacctgaact
    41581 tgaactatca agatttggtt tagaatttga gaatcgatgt ttataaaggg agggcaaaaa
    41641 atgtgctttt ttacaacttt tactatcatt atgattgcag tactttttat ttttctagat
    41701 atagcaaagc gaaatacagc atttttatta tatcgggttt tacttcgagc aggtctcata
    41761 acatttatat cgatcgttgg attttttcta ttcacagtta ttgtatttat ttggagaact
    41821 acagcaccac cattgccaga gattacatat ggagaatttc cgtttagtct tgaatatgaa
    41881 atagatgagg aaagaatttt gattgaagat acggtgattg cagaatttat aggaagtttt
    41941 agagggaatg tgacaactca acctagacga agttggagta taaaactttt aaataatagt
    42001 ggagagttga aagcatttca ttatgaattc ttaataatag aaagtgaaga tgtaacaatc
    42061 actttaagta gtggctctgc tggatatttt atgaatgatg gtggaagcaa gccacagctc
    42121 acagtccgtg atcgtattgc aggttttatg catttttctc actatgactt agaagaagct
    42181 cgtaaaatac ttgcagaata tgggatcaca ttaatcaatt gggaatatgc gccaccgata
    42241 agaaattttt tcaatgatta ttattagtct tataaaaaac agaatgtaaa atttatgtgc
    42301 gcgagtattt agttttccat tttttgtttg atagatatat agcattgttg aagcatggga
    42361 tagtggtgcc aattttatca atgaggttgg tcaggcagtt aacataattg gtgatgctat
    42421 aaatccattt aattggaatt ggaggaatta ggtgtggaaa cagtgagatt atggatattc
    42481 atgaaaaatt ttcgttcggc tttgagatat gttattctgg aatcaaaatc aggttattat
    42541 ctgattgaca cagatcacta tactttttgg ggaggctacc tttttccgat ttttaattgg
    42601 ataatccccc ataaggttat gaaaattaac ttgcctgcag aagaaataaa tagattaata
    42661 ctcaacaaag atttacaaag gaaaagagag aaagaacatt ctaatgttgc ttggataggt
    42721 ccagcatttg cagtagtgac ttcttcaatt gtcggtccta tgctagagag tatttatagt
    42781 gatttttcta ttggaatacg ttatttattt gttactttaa gtatagctgt tcttttgtgc
    42841 ataaagggtt atgttagcaa gaagaataaa gatattttaa cactaatcgg tgtaaacaat
    42901 ttatctagta ctaaaattat actgtttcct gaatctataa aagaaggatc aaggttgttg
    42961 ataattttta taatgcttat actgttttct gttttatttg gggtgatgtt tataga
//
LOCUS       DAFGZE010000019        41408 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.019, whole genome shotgun sequence.
ACCESSION   DAFGZE010000019 DAFGZE010000000
VERSION     DAFGZE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.019"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            214..498
                     /locus_tag="LTU54_001068"
     CDS             214..498
                     /locus_tag="LTU54_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HBL8295795.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="LTU54_001069"
     CDS             complement(779..1198)
                     /locus_tag="LTU54_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HBL8295796.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="LTU54_001070"
     CDS             complement(1200..1784)
                     /locus_tag="LTU54_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBL8295797.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="LTU54_001071"
     CDS             complement(1831..3306)
                     /locus_tag="LTU54_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBL8295798.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="LTU54_001072"
     CDS             3487..3798
                     /locus_tag="LTU54_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295799.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="LTU54_001073"
     CDS             complement(3913..6249)
                     /locus_tag="LTU54_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8295800.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLPSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="LTU54_001074"
     CDS             6391..7059
                     /locus_tag="LTU54_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295801.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="LTU54_001075"
     CDS             7062..8105
                     /locus_tag="LTU54_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8295802.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="LTU54_001076"
     CDS             complement(8342..9223)
                     /locus_tag="LTU54_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8295803.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="LTU54_001077"
     CDS             9382..10110
                     /locus_tag="LTU54_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBL8295804.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="LTU54_001078"
     CDS             10266..10583
                     /locus_tag="LTU54_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL8295805.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="LTU54_001079"
     CDS             10576..11184
                     /locus_tag="LTU54_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBL8295806.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="LTU54_001080"
     CDS             11239..12030
                     /locus_tag="LTU54_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBL8295807.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="LTU54_001081"
     CDS             12168..12656
                     /locus_tag="LTU54_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HBL8295808.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="LTU54_001082"
     CDS             12707..13579
                     /locus_tag="LTU54_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL8295809.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="LTU54_001083"
     CDS             13755..14117
                     /locus_tag="LTU54_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL8295810.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="LTU54_001084"
     CDS             14126..14878
                     /locus_tag="LTU54_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295811.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="LTU54_001085"
     CDS             14892..16694
                     /locus_tag="LTU54_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295812.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="LTU54_001086"
     CDS             16971..18011
                     /locus_tag="LTU54_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL8295813.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="LTU54_001087"
     CDS             complement(18063..20207)
                     /locus_tag="LTU54_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL8295814.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="LTU54_001088"
     CDS             complement(20564..21937)
                     /locus_tag="LTU54_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8295815.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LTU54_001089"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="LTU54_001089"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBL8295816.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LTU54_001090"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="LTU54_001090"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBL8295817.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="LTU54_001091"
     CDS             complement(23385..24398)
                     /locus_tag="LTU54_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL8295818.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="LTU54_001092"
     CDS             complement(24479..25078)
                     /locus_tag="LTU54_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL8295819.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="LTU54_001093"
     CDS             25264..25965
                     /locus_tag="LTU54_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8295820.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="LTU54_001094"
     CDS             25975..28686
                     /locus_tag="LTU54_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295821.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="LTU54_001095"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="LTU54_001095"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBL8295822.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="LTU54_001096"
     CDS             complement(30825..32006)
                     /locus_tag="LTU54_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL8295823.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..33781
                     /locus_tag="LTU54_001097"
     CDS             32258..33781
                     /locus_tag="LTU54_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HBL8295824.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33850..34578)
                     /locus_tag="LTU54_001098"
     CDS             complement(33850..34578)
                     /locus_tag="LTU54_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295825.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34591..35871)
                     /locus_tag="LTU54_001099"
     CDS             complement(34591..35871)
                     /locus_tag="LTU54_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL8295826.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(36012..36614)
                     /locus_tag="LTU54_001100"
     CDS             complement(36012..36614)
                     /locus_tag="LTU54_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL8295827.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36859..37065
                     /locus_tag="LTU54_001101"
     CDS             36859..37065
                     /locus_tag="LTU54_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295828.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37377..38096
                     /locus_tag="LTU54_001102"
     CDS             37377..38096
                     /locus_tag="LTU54_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL8295829.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(38177..38860)
                     /locus_tag="LTU54_001103"
     CDS             complement(38177..38860)
                     /locus_tag="LTU54_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBL8295830.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            39078..41264
                     /locus_tag="LTU54_001104"
     CDS             39078..41264
                     /locus_tag="LTU54_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBL8295831.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        13433 a         7338 c         8312 g        12325 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaaggaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaacttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atttaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataaa ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaaatg aaagtcaaga agtattgctg aaagaaatgg agaattggcc
    33721 aacattttat ccagatacaa tggatggacg tgaaattgca caggaaatgc ttttgcactg
    33781 ataggcaaaa aagaaccgaa gggtttggat cggctggtca tttagtcggt ccaaactatt
    33841 cggttctttt tatgaaatca atttctttaa tccacggata gaggctggga tggtctcgcc
    33901 gtttgccaac gtgacttctc tggttttttt attaatcgac cgcacatttt gtgtattgat
    33961 aacataggat ttatgacaac ggataaaatt ccgatgcatt ttctcgattt ctttgatctt
    34021 ctcatagaat tgtaattggg catgagtcaa atggacttcc agtttatgtg gcgctcctag
    34081 cgaagtagaa aaatattgga tgtctttgat cggtaaaaat ttgatctcat cattaaactc
    34141 tattttgaag atatcttttt ggatttggga catttgaaaa tcttcgaaaa ttttgctcag
    34201 gatatgctcg acattttctt ttaaatcata aagatcttct ttgacgatat aatcagtcgg
    34261 ttcgatgttg ctttttaaga ttttcatcga catttccgta tgggaagtca catagattat
    34321 ttttcctaaa ggatctaatt cacggatcaa tttaccgatc gctacaccat ccatttcact
    34381 gtcttctagt tcgatatcta aaaagtaaat accaaaagaa gaagttctga tcttggctgc
    34441 attgagaagt tcttccggat cagaggtaga aatatggaca taactgtcta agttttcaat
    34501 catgattcta tttttgatca ccgttgataa aacttctaat tgttttggat cgtcttcaca
    34561 aatataaatg ggaatcatat cattactcct ttattttatt tgtatataaa tcgtttgaga
    34621 aaaacgctgg ttttcaattg atgtttcaag gaaaagataa ggattggtct ttgtcaattc
    34681 atccaagata aacaagccaa ggccttcgtt ttttggattt gtttttgttg agaacccttt
    34741 ttgtttcaac tggtttaacg gtaatgtatt tgttgttgta ttgatcacac gaatcatcac
    34801 ggaatgattc ttcttgagta tggtcaattc gatttttttc ttctttgcag cactagctcc
    34861 ttcaatcgca ttgtccaaga taataccaag catacgtaca aggtttaccg tatgtttcgt
    34921 atccagttcg attggctcag gaaaatgaag ctggacgatc aacccttttt cctgtgccat
    34981 catgagtttt aatgacagta aactacggat ctcaggaact ttcatattat ccaacatact
    35041 tagattggcc ggaaaaaggt tcatcatctg gcgtgtaggt aatatcgttt gatcataata
    35101 agaccgtaat ccttctaagt cgccttctga aatatagctg tgaagagaaa aaagcagatt
    35161 ttggtaatca tgtttgaatt ctctcaagtc ttgatagttt ttttctagat ctttgatata
    35221 atcctgttgc atcctgactt gttgctctaa taactgatca gctaatttct tattagagat
    35281 tttccaataa taaaccaaga tgatcaggcc aacacacaga tatagaatga aaaaactcgt
    35341 attcaaagct aagacatcag gtgtttcacc agaaaaacgg gtataaaaaa taatgatata
    35401 gtatgtaaat aatatcaatg tcccgatcgt accgatcata cggcggtcca cagtgtgaaa
    35461 ccaagtctct aacgattttt ttaaacaaaa agctaataaa gcagataaaa aagcggataa
    35521 gacaatgtga aaatactgca atgaatctcc tggttcaata accgaagatt tcaaaacaaa
    35581 aaaatcgccg atgcttgtta agtgatctcc taaaagcgac gtcaataacc caaaaggaag
    35641 cgcgtaaacc aattgttgac gcgaagtcga aaagaagaaa aaagtaagca tcagcattcc
    35701 tatagcaatt agagcagtaa aaatccctaa aatcgaatag acccatccaa tcaaaataca
    35761 agaaatcaga atgactcccg caaacagata tttgtatcgg atgtgatggt caatcaataa
    35821 gatacaaatt gtgtaaatca aatattgcaa cgctatggac cataaaaaca taaagtacag
    35881 ctcccattga tttttaccaa attatagcac aattggcgta ttattttttt aatttttcag
    35941 ggattttgaa ttcagaactc ataccaaagc agcagccaga tacaaaaaag tcagataagt
    36001 tagttaattg tttaatgatt gtttttttca tgaaagattc ctccaaaatt catcaagtag
    36061 tttgtaatag gaagcaacgt agctatttct aaaacaatcc caagtagcaa ataggtagaa
    36121 aagtaagaag ctgagaaaaa taacgtaatg agagaaaaaa ttagaaacat gctgatcgcc
    36181 ttctttaata atgggggacg tttctcgggt tcaatgggat tgattgctgt acctcttgat
    36241 ccatagcgaa gcagtaacaa aaagcagctg attaacaaaa agaccatcat tgggaaggag
    36301 atagtcaatt gcagatgttt catcaccaag ggaacgcccc ataaataaag tagtgtccac
    36361 agcagacaaa cgaattcaga gtcggaatgc aatccataag catatctgcg gatgcagata
    36421 taagaaagaa aggtgattcc tgtttccgcc catgagcctg tcaccagaga aaagactaga
    36481 acgaccgcta gttttccaag attattcaat aaaacttcta agacaaattt tacttggaca
    36541 tacaccatat cttcttccga tttgtctccg ctgaataagg cttctaataa tttgttcaca
    36601 actttatctt tcatctcata cgtacctcca ccacaaattt tatgctaaaa aaaataaaaa
    36661 acgtcttttg tttcataaaa agtggttttg acatcaaaac cgtcatgatc tcctaaaaaa
    36721 atcttcgttt tccaagaacc ttaccaaaaa aagtgacagt tttgatacga aaagtacttt
    36781 ttatgacaag tttgtgcttt ttcgagtgat ttatgttaaa aaattactga ccttataaaa
    36841 ggtgatggag ggattcttat ggaaaatgaa caattgtttg aagtcggcga aacatatgca
    36901 tttaaatctg atttatttga atcaccaata aaaggaaaaa tagaaaaaat ctatgaaaat
    36961 agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca atgataccgc tcataatctg
    37021 cactggcagt tagttgtacg caaatcaagt acaatagaag aataaaaaca gataaaatgt
    37081 tgagagcata aagaggttgt gagtctcccg tttagctctg agttacaagg aataatgtta
    37141 aaaaacagct tttcatgttt tatgaatatt gccttgtgac cgaggtgttg gcaggagaat
    37201 actgtttatt tgaaaaaagg gctgcgacaa gacttttgtc acagccttta ttttcatgaa
    37261 taggagttac ttttggctac ttttagagaa gtagctttta aggagcagat aaagtcaagg
    37321 cttttatttc tataaatgaa gtagtaaaat gaagaacaga ttttattaca ggaaggatgc
    37381 cgataattgt gatcattgtt gcaggaacga tcggtgcagg gaaaagcaca ctaactgaaa
    37441 tgcttgccca agatttagaa acaaaacctt tttatgagaa cgtggaagat aatgaagtat
    37501 tgccgctatt ttattctaat ccagaaaagt atactttttt attacagatt ttctttttga
    37561 acaaacgctt tttggcaatc aaagatgctt tcagccatga tgacaacgta ctagaccgct
    37621 ccatctacga agatagtatg ctgtttcatt tgaatgcaga tttagggcgt gtttctgaag
    37681 tagaagtaaa gcagtatgaa ggtcttttag aaacgatgct gaaggagtta gaagaaattt
    37741 cgccacaaaa gaaaccagat ctactggttt atattcgtgt atcctttgaa actatgttag
    37801 cgagaatcaa aaaaagaggc cgggaatatg aacagctaga acaagatcct gaactctatt
    37861 cctactataa agaactcaat cgacgatacg aagagtggta tgaacagttt gatatatgtc
    37921 ccaaaatagt gatcgatggg gacaaatatg atttcgtagc cgatccggca tgtggagaac
    37981 aaatcgtaca agaaatcaag atgcgggcaa agaaaatgac agaagaggca gatgcccagt
    38041 cgatccgcta cactacaaat aacccgaaag caaagggaat ctttacaact aaataatata
    38101 aaaactaagc aaattgctca ctcgcagata tttctctgtg agcagcatct tgcttagttt
    38161 ttttgattca ttgagatcaa atagtgaacc gattcgtatg ataaaattgc cataaatgaa
    38221 atgtctgagt gagggccatg atcaaaaata aaatggaaac gatcaaaatc gaatgggcgc
    38281 caaacagatg atgacagaaa acaaggacaa tagctcctgc gataaaggca agaacgacac
    38341 ttagatagtg gaaaaatcga tcaaaatctg ctttttcttt agaaatacaa taatcatata
    38401 agttcgttgc tacccctttt aaatttccag ttgtaaataa attagtgtag gcacgcccgt
    38461 tgattttatc aaaagctacc cactggatcg atgcagtaaa agcaatcaaa atcgttacta
    38521 atgcgggatg ctcatggacg ggtgtcaaac taacaaagaa aaaaatcact gcttcataat
    38581 atagaacaaa caagcgccag aaatgttttt ctttttttct aaaataatca attagatact
    38641 ttgttagaag aatccctaag aaaaagaaaa gggtagacaa tactttttta ccaacactta
    38701 gccattctcc gtcaaatgct tggatgatcg atagaatgat attgccagtt tgagcagagg
    38761 caaaagcacc atattggata taagtataac tgtccatggc tcctccgata aatgtcagca
    38821 aaagtcctat tgtacgcccc tcgtgaaagg aagtctgcat ttttttcatc accttttatg
    38881 aatatttttg tcttccttat cttagcataa acgattgaaa aaaaggaaaa attttcagaa
    38941 aactaaaatc atatcgcttc ccattttcat ttttagaaag gtctgttagt ataaagaaaa
    39001 aatagacaac gaaactttaa ataaccaact tgaaataaac aaattcaaag aagaaagatg
    39061 ggaaggaaat caatcagttg aatgaaaaag aaattctccg attacaaaaa gaattgaaac
    39121 gctgggagaa caagtataaa aagcttcaac aagtctttcg gctacaagaa gaaatcattg
    39181 ctacctatga agcggcttat ggcgaagcac ccggacttat tgaggaagaa gtacaagtaa
    39241 aagaaagtga aggaaacagc tctttcttta aaatgatgga gaaacgttcc tataatcgct
    39301 tcaatcaaga acaaaaagca gctatcacat atgatatgaa gaaacatctt cgaatcatag
    39361 caggagctgg gagcggcaaa acgcaaacaa tctgtgcaaa agctgtttat ctgatgacgc
    39421 aaaaacaagt agacgaagaa cgcatactaa tgatcacttt tacaaggaac gctgcaaatg
    39481 aactaaaaaa acgagtagat aatttcagcc agagaaaaac cgctgtccat atcggaacat
    39541 tccatagtat cttttttagg atatacaatg aaatttgtcg aaaatttcct gaagtagcaa
    39601 tgcaaggaat acaaggagac ttttcaaagg atactgcaca aaaagtgaat gctgttttgc
    39661 aacagctgat acgtaaatat aatctttata tatttgatca atatggagaa aaaacgattg
    39721 cttctcggtt agactattgg cagaatatga acctttcagt agaagaaatg gttcagttag
    39781 ttaaagaaaa atatgattcc attgataaga atgcacgaca gccgatatca gaacgactgt
    39841 atggattgct cacggaactc caagaacaaa aaagaagcca gcaattgcta gagttcaatg
    39901 atgtactcca aaatctgaaa agcgctttgg agaatgagga gatccagcat tatatcggcc
    39961 aaaaatatga ttatctattc attgatgaat ttcaagatac taatccactt caatgggaaa
    40021 tcgtgaaacg gatgacaaag gaaaacagga tcaagttgat tgttgttgga gatgatgatc
    40081 agagtatcta tggattcaga ggatcagagc cagcatacat caagaacttt gaaaaagaat
    40141 atcctaccaa aactcttttc ttattaacta attatcgttc tcgggcagct attgtccaag
    40201 gagcaaatag attgatcagc tataataaaa atgatcggat accaaaggcg atgattccag
    40261 cacaaaaaga agcaggtgtt atggaagcct atttgttttc tgatactaaa gcagaaagtc
    40321 agtggctaat caaacaaatt cagacattta ttcaaaaaaa tgggaaatat aaggaatcaa
    40381 tcatattata ccgatcagct tcacaaacgc agcagcttgt ccaatctctg ctgaaaacga
    40441 atattccttt tgtactggaa gcagatagtc catatgaagg tatctttggt atcaagtctt
    40501 tccaacattt ctatcaaaaa attgttcatt ggcaaacagc actgaatctg caaaagaaaa
    40561 atcaggccta ccagcaattg ctccggcagc tgatggcaga ctgctacctg aaaaaaagtg
    40621 aatgcgatca atacttttct cctaaatatc aaaatatagc cgttgcagat tatattttgt
    40681 cgataagccc caatttaaaa tcgaaatctg cagaaatccg agagtttgag gaagcgttgc
    40741 gtaaaacaga ccagcatcaa gaaatctatt cgcttgttca aagctatctt catctgccac
    40801 gtatagctaa ggaaatcgac cagagtgatc aagaatggat acgagaagaa gtaaagcagc
    40861 atgctacttt cagaagactc caagccttat cgcaagaaac ccaagcgaca gctaaagaat
    40921 taaaaacccg cttgattgct tacagacaag gaaaacttga tgcactatgt atccagagca
    40981 ttcacaaaag taaaggtctt agttaccgaa atgtcttttt gattggttgt aacgaaggaa
    41041 gtttgcctta taacggagcg acagaaaaga aagtaatcga ctttaatgaa ataaaagcag
    41101 aaccagtaac gacgatagaa gaagaacgaa gactatttta tgttgcgatg actagagcaa
    41161 gaaatcgtct gtatctatgt gtacctcaaa tgaaaaattc aagaaaattg aaagtatcga
    41221 gatttatcaa agaaaccggg agtcgtgtga aaaaaggaaa gtgatagaag agagggtgtg
    41281 acagaagtgg tcagtttcaa gaaagaagaa ggaatgtacg aaaattgctt ctcaaatttt
    41341 tgtgaaattt cagcttattt tcgaagaagc tatttctgga acacagttta ttcggaaaag
    41401 ggaggtgt
//
LOCUS       DAFGZE010000020        41011 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.020, whole genome shotgun sequence.
ACCESSION   DAFGZE010000020 DAFGZE010000000
VERSION     DAFGZE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.020"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            72..1412
                     /locus_tag="LTU54_001105"
     CDS             72..1412
                     /locus_tag="LTU54_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8295832.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1630..2073
                     /locus_tag="LTU54_001106"
     CDS             1630..2073
                     /locus_tag="LTU54_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL8295833.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="LTU54_001107"
     CDS             complement(2577..3995)
                     /gene="nhaC"
                     /locus_tag="LTU54_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL8295834.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4398..5621)
                     /locus_tag="LTU54_001108"
     CDS             complement(4398..5621)
                     /locus_tag="LTU54_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBL8295835.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5652..6491)
                     /locus_tag="LTU54_001109"
     CDS             complement(5652..6491)
                     /locus_tag="LTU54_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL8295836.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6508..7188)
                     /locus_tag="LTU54_001110"
     CDS             complement(6508..7188)
                     /locus_tag="LTU54_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295837.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7190..8263)
                     /locus_tag="LTU54_001111"
     CDS             complement(7190..8263)
                     /locus_tag="LTU54_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8295838.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8319..8557)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8600..8833)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8910..9581)
                     /locus_tag="LTU54_001112"
     CDS             complement(8910..9581)
                     /locus_tag="LTU54_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8295839.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9668..12346)
                     /gene="mgtA"
                     /locus_tag="LTU54_001113"
     CDS             complement(9668..12346)
                     /gene="mgtA"
                     /locus_tag="LTU54_001113"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBL8295840.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            13005..13442
                     /gene="ndk"
                     /locus_tag="LTU54_001114"
     CDS             13005..13442
                     /gene="ndk"
                     /locus_tag="LTU54_001114"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBL8295841.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13464..14330)
                     /locus_tag="LTU54_001115"
     CDS             complement(13464..14330)
                     /locus_tag="LTU54_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8295842.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKETSNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(14490..16583)
                     /locus_tag="LTU54_001116"
     CDS             complement(14490..16583)
                     /locus_tag="LTU54_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL8295843.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(17332..17955)
                     /locus_tag="LTU54_001117"
     CDS             complement(17332..17955)
                     /locus_tag="LTU54_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295844.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHVGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(18114..19511)
                     /locus_tag="LTU54_001118"
     CDS             complement(18114..19511)
                     /locus_tag="LTU54_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8295845.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(19543..20514)
                     /locus_tag="LTU54_001119"
     CDS             complement(19543..20514)
                     /locus_tag="LTU54_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295846.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            20888..21313
                     /locus_tag="LTU54_001120"
     CDS             20888..21313
                     /locus_tag="LTU54_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295847.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(21801..22502)
                     /locus_tag="LTU54_001121"
     CDS             complement(21801..22502)
                     /locus_tag="LTU54_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HBL8295848.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(22518..22958)
                     /locus_tag="LTU54_001122"
     CDS             complement(22518..22958)
                     /locus_tag="LTU54_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HBL8295849.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(22958..23533)
                     /locus_tag="LTU54_001123"
     CDS             complement(22958..23533)
                     /locus_tag="LTU54_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HBL8295850.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(24381..24860)
                     /locus_tag="LTU54_001124"
                     /pseudo
     CDS             complement(24381..24860)
                     /locus_tag="LTU54_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(25019..25720)
                     /locus_tag="LTU54_001125"
     CDS             complement(25019..25720)
                     /locus_tag="LTU54_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8295851.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(25828..27237)
                     /locus_tag="LTU54_001126"
     CDS             complement(25828..27237)
                     /locus_tag="LTU54_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBL8295852.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(27285..29150)
                     /locus_tag="LTU54_001127"
     CDS             complement(27285..29150)
                     /locus_tag="LTU54_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8295853.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(29271..30122)
                     /locus_tag="LTU54_001128"
     CDS             complement(29271..30122)
                     /locus_tag="LTU54_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8295854.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(30432..31145)
                     /locus_tag="LTU54_001129"
     CDS             complement(30432..31145)
                     /locus_tag="LTU54_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL8295855.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(31213..31539)
                     /locus_tag="LTU54_001130"
     CDS             complement(31213..31539)
                     /locus_tag="LTU54_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL8295856.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(31662..31889)
                     /locus_tag="LTU54_001131"
     CDS             complement(31662..31889)
                     /locus_tag="LTU54_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL8295857.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(32211..33464)
                     /locus_tag="LTU54_001132"
     CDS             complement(32211..33464)
                     /locus_tag="LTU54_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL8295858.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            33525..34265
                     /locus_tag="LTU54_001133"
     CDS             33525..34265
                     /locus_tag="LTU54_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8295859.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(34351..35109)
                     /locus_tag="LTU54_001134"
     CDS             complement(34351..35109)
                     /locus_tag="LTU54_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HBL8295860.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(35106..36134)
                     /locus_tag="LTU54_001135"
                     /pseudo
     CDS             complement(35106..36134)
                     /locus_tag="LTU54_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(36154..39321)
                     /locus_tag="LTU54_001136"
     CDS             complement(36154..39321)
                     /locus_tag="LTU54_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295861.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(39545..40429)
                     /locus_tag="LTU54_001137"
     CDS             complement(39545..40429)
                     /locus_tag="LTU54_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HBL8295862.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(40524..40961)
                     /locus_tag="LTU54_001138"
     CDS             complement(40524..40961)
                     /locus_tag="LTU54_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295863.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        11728 a         8628 c         6980 g        13675 t
ORIGIN      
        1 gtcattgtta aatattgcta tttattctta atataaattt tgattgtgaa tttaaaagaa
       61 agaggtcatt tatggaagat aaaaaattga aaaaacaact tagctccaga catatcacca
      121 tgttagcttt aggtggagca atcggtgcag gtctatttaa aggaagtggc gaagcgattg
      181 ggatcgccgg cccttctgta ttgattgctt ttttgattgg tgggctgatt ttatatatcg
      241 tgatgaaagg tctgggaaaa atcgttttaa gcggtggcga cacgcatcac ggattatctg
      301 gtttgatacg cccttatcta ggtgctcatt ctgctgattt tactgattgg gtctactggt
      361 ctatgtggat tatcaatatc atcgctgaag ctgttgcagc tgcttccttt ttgcagttgt
      421 ggttcccgca cattccagcc tgggtatttg tttttatgtt ggctgtttta actacgatca
      481 tcaatcttta ttcggtacgg ttattcgcag aaacagaata ttggctggct tttgctaaaa
      541 tcagtgtcat tatcttactg atcatttttg ctacttatct tgtcggtcag caaatgttgg
      601 gcacaggcgt tttccctacc cttcagcaat tgactgacca tggtgggttt actcctcatg
      661 gtatgaaggg aatcatcagt tctttgcttg tagttatcta ttcttacggc ggttctgaac
      721 tgattgctat cacggtaagc gaagcagatg atcctaaacg tgcgattcct aaagcaatca
      781 aaggtgtcat gggaagaatc atttcctttt acatcattcc tttgtttttg cttttgattc
      841 tttttccttg gaatacttta gcaggcacga atgtcagtcc ttttgtgatg gtatttgaaa
      901 aaatgaatat cccatttgca gcggatatcg tcaattttgt gattgtcctt gctttgttct
      961 catctatcaa ttcgggtgtg tatgcatctt caagaatttt gttcttccgt ttgaaggatc
     1021 gaaaaggttc ctctagaaaa ctagctgttt taaacaagca tcaagtaccg caacgtgctg
     1081 ttttattttg taccagtgtt ctttatcttg gtgtagctct ttcttatttt gtcggtgaca
     1141 aattatttgg ctatctggca ggttcccttt cgtatacagt attattgatc tggatcatta
     1201 tcagtgcggc atcctttgta ctggcactaa gaaaaggaac attatgggat aaaggaatca
     1261 gcttttttgc attagctgtc ttaggattga ttttccttgg tatcctgttc acgaactcca
     1321 ttggggtaac tgtattgact ggcctgcttt acctgttcat ttatttcagt tatcaaaaaa
     1381 agaatgatgc gttcgtattg acgaatgaat gaaatcgata gaaaatttta ttttatttat
     1441 gtggaataga tggagctatt gaagaaagga gttgtgacaa aagtcttgtt gcaactcctt
     1501 tttccgaata aacagtctca tgacctcttg gtaaacggtg ttcaaaaacc ctctgtgcaa
     1561 aataattgat tgttctccat gctcatgaca tgatagaaga aaggaatgaa aaaaaggagt
     1621 ttgttttaaa tgaaaaagta ttggaagttt ttgttgatga tcggtgtttc aacggtagtt
     1681 atgtttggta tgatgtactt gaatgtctat gcgctcgatc atatcttctt cagtcgtaca
     1741 cgagtattca tggcgttgat gatgggtgca gtcatggcag tcatcatgct gttgttcatg
     1801 tggaaaatgt atgaaaacaa aaagataaat gttatgatct tagccgctag tcttcttgtt
     1861 tttgccggtt ccttgtttat ggtacgaagc caaactactg tcaacgacac agcttggatg
     1921 aaggccatga ttcctcatca ttctatcgct atcctggcaa gtaaacgagc tgatttaaaa
     1981 gatcctgaag taaaaaaatt agcggaagaa atcattcagg cacaagaaga agagatcgac
     2041 aaaatgaaaa aaatgatcaa agaaatggaa tgaccattat ccgtccaaat aaaaaaagag
     2101 gatgttccag aagttatcag cttcaagaaa taagaagaag ctacttctgg aacgccgtga
     2161 ataaggggtt gtgaaaaagc tgccctgcat caagtaataa gaacagccaa acaaaaatag
     2221 cttctcctat ttttcgtttg gttaggctta ttaccgcaga tgcaagcttt tgaacaccgt
     2281 ttattaagag gttgtaaaat cagcgttctg acctgagtat tagagagaca aatggaaaaa
     2341 tagttcctca tattttgcca ttttttgagc taataccgag ggtcagctga ttgaacaccg
     2401 tttatcgaga ggttgtgaaa aagtcgctta gtttcgcgta ttaagaaaaa ttttgtgaaa
     2461 tggctttcca catttttgta taaatttggc ttaataccga gaaactggct tttgaatacc
     2521 gtttattcgt aaaagggatt gtgacaaaag tcttgtcaca atcccttttt atgtttttat
     2581 tgttctaatt gcgctgtctt gcttactgct tcttgcggtt tcacaaattt ttttgagatt
     2641 ccccaacctg tgaaggcact gaccatcgaa aacacaggag aaagcaaact gaaaaatacg
     2701 aatggcaagt aagtgagtgt aggaatacca aatgtacttg ctacgaaact tccggcaatt
     2761 ccccaaggca ctaagtagtt gattactgtt ccaccatctt ctagcgttct acctaataca
     2821 gttggatcaa atcctgcttt ttcataagtt tctttaaatg cattaccagg cagaataaca
     2881 gataggaatt gttcaccaat aaaaatattt actccgatac ttgtcatcaa tgttgtaaaa
     2941 atcaaatttg gtgtgttcgt catctttctt gatactcgtt ccatcacggt tgagatcaag
     3001 ccaaattcga tcaacaatcc acctaatgac aaagttagaa taatcaatga cacagtaggc
     3061 atcatacttt cgattcctcc gcggctaagc agtgaatcga tttgttggtt tccagtattt
     3121 gaaacaaacc ctgattcgat gaccccagcc aacttcgtta aggaagtttg tgggttttga
     3181 ataaaaatca ttcctactgc tacagcgata ttcaacaaaa tcgttaaaat tgccggcacc
     3241 tttttccatg cacacagtaa cattaaaaat aaaggaatca gtgaccagat tgaaatagta
     3301 aagtttgctt ctagaacatc agtcacttca cgaatagatg aaagacttgc ggctttactt
     3361 gtgtgtccta aaatcatgaa taaaaagaaa gaaacaacaa atgccgggac agttgaccac
     3421 atcatatgct tgatgtgttt gaacaaatcg gcatcaacaa tagctgctgc tagatttgtt
     3481 gattcggata atggagacat cttatctcca aaaacggcac ctgaaacaat agctccaaca
     3541 accagcgctg gatttaagcc tagtgttgtc ccaataccga aaaatgcaat cccaatcgtt
     3601 gacataacag taaaggcact ccctacagcg ctacctacga ttgcacacac aacaaataca
     3661 gaagggacaa accactggac actgatcaat ttgaacccaa tgaccattag agttggaatg
     3721 atccctgctt gtatccatac agcaatcaat gaaccgacta aaataaaaat aaagattggg
     3781 atgattcctg gtttgatgcc attgatgatt ccttcgtgca gccaatctgt cgggtatctt
     3841 tttatcactg catacaacat gaccattccg atcgctgcta agatcgtgat attaggtgag
     3901 acctttaatc cgacaacgcc aatcgcaatg cctaaaacaa taaccattaa tacaagtgtt
     3961 gcttctaaaa aacttggtgt tccttttttt cccatgataa tctctccttt tttagttgtt
     4021 cttatgttct aaaaaagtta agatatcgga ttactccgcc gcaattcatc cgcgattcct
     4081 cctttataaa aataaaaaga cctgttttaa actaaggacg aatgactccg cggtaccacc
     4141 ttaattaaaa acagatctgt ttttcacttt caaactttta ttcaaatgta tggactttcc
     4201 atgtaattcg tcaattaggc ttgatcggtt cacagcgacc accgactttc tgaacaaaac
     4261 acctaaccgc tactgcgtga aaagttgact tgtacgaagt ttaccttcca taagatcatt
     4321 tgtcaatgaa aaagaaaata ttttttcatt tttctgaaaa ttatttttat aactttcaca
     4381 cacaagttta ttttttacta gaaagtagcg ccctcttgtt tttttcttgc tactatttct
     4441 tgatgttctt gcttgattct ttctaatagc tttggctctt tcaacagatc aagagctgta
     4501 ttggcgagta gttctgctgc aatcgcaata gaagcaaggc ctttgtcact tttagcagca
     4561 gctttgaatt cttctgtgtg tccggcaata tatgtctcag agattgctac cgtaggctgg
     4621 attgttggta tgacttggct gacatttcct acatcagaag aaccgagact tgttctttgt
     4681 tccgtttcta tttcttcatc tggaacacct gcttcttttg catgctggaa gaataagtca
     4741 tcaaaagaag gagtaacgat gacatcatca actgcatttt ggaacaatcc aaactcaaaa
     4801 gtcgtgcccg tacttatcgc agcacctcgt acgatatttt ccactttttc atatacttta
     4861 tctaatgttt gccgatttcc tgcacgtaaa tagaaacgtc cagcagcgta atctggaatg
     4921 acgttggcag ctactcctcc gtctgtaatg atcccatgga tattgacatc ttttggcaat
     4981 tgcaagcgta gagcgttgat tccattaaac acttggatca atgcttccaa agcatttact
     5041 cctttttcag gtgctgctgc tgcatgggaa gcgacaccgt aaaatttgat gtcgactgga
     5101 tcattcgcta aagattctgt cgttttccca taacgaaaag ctggatggac gcataacgct
     5161 acatcgacat cctcaaaaaa accttctcga acaaagctgc ctttagctga tccgttctcg
     5221 cccccttctt cccctggggt gccatataca cggatttctc caccagtttc atcaatgaat
     5281 tgcttcacta tgctggcggc taatacggag taagtaccaa acaaattatg cccgcatgca
     5341 tgaccaatac ctggtaaagc atcgaattct gccaaaaagg caattgtggg accgggttta
     5401 ttggaaatat aacgtgcatc aaaggctgtt cggtgtccag ctacttcttt ttctacccaa
     5461 aacctttctt tttctaattg tttaatcaac gctgctgagg aataaaactc atagttactt
     5521 acttcaggat gttcgtggat atctagagca agtgcttgat attctggtaa acgatattgg
     5581 attagatcct tcaattggtt tgttgagatt gttgttgtca taaaaaattc ctcctatgct
     5641 agtcattttt cttattttct cccgaatgtt tcccatgcag gcacgcttga tccttttgat
     5701 gttttctcga ttactttgac tgtgtcttct gtttgataag cgtctactat tttttgatac
     5761 gttttattgt tttcatcttc ttttcttgca acgatgatat tcacataagg acgtgcatgg
     5821 tcatccactg gttctaagaa gatggcatca gaagcaggat taaatccagc atctacagct
     5881 acaccgctat tgatcactga aatgtctaca tctgacaaag cacgtgctgt ttgggaagca
     5941 tctaattcag aaatgtctag attttttcta ttctcggtaa tatcagaaac agtgggcgct
     6001 tgtccttttg ccttatccac tttgatcaat ccagcagttt gcaataacag aagagctctg
     6061 ccgccatttg taacatcatt agggattgct acttttgctc cgtctttgat atcagctaca
     6121 tccttcaaag tgttagaata aattcccagc ggtgcattga cagtatttcc gatcgatact
     6181 aaatcagttc cgtgttcttt attgtaattg tctaaaaaga attgatgttg gaaagaattc
     6241 aaatcaatcg atccatctgc taaagccaca tttggttgat tgtaatctgt aaatttcacg
     6301 tattccaaat taatgttctc ctttttcaaa cggtctttca cgttgtccca tacatctgta
     6361 tcttctccaa taatccctag ttttacgata gtttcttttt tgtcagttgt atctgctttt
     6421 ccttggctac accctgccaa ccaaataatt gctacaaatg taatgactac aaaacgaata
     6481 atttttttca ttttttctcc tccactttca atgtgtaatt tttttaatca gttgattact
     6541 gataaattga cttaaaaaaa ctaagattag aatcaataat gtagccacaa tcgttacatc
     6601 tgtttggaat cgattatacc ctctcgatat agcaagattc cctaatccgc ctgcacctac
     6661 tgctccagcc atggtagtca gtccgatcac gttgatgata gtcaacgccg agacccgaat
     6721 gataccaggc aaaccttcta acaaatacac acgtaaaatg ataccgattg gactggttcc
     6781 catcgcttct gccgcttcga tcaccccagg atcaacttct agcagtgcgt tctcgatttg
     6841 acgagcaaaa aacggaacaa ctccaaaaat caatggcact aaagctgctt tcgcaccaat
     6901 cgtcgttcca actaagaaac gagtagtcaa tgctaaaaga gaaagcaaga taatgaaagg
     6961 gatggaacga atgatattga tcagcttatc aagtatttca aatagcagac ggttagctag
     7021 taatccatta gggcgtgtca ctactaacgt cacacctaag atgattccaa tcatcccgcc
     7081 aatgaatgct gtccaaaaag acatatatag tgtttgcttc gtcgcttcga caaattcatc
     7141 cggaatcaaa taaacattgg gcaaatatgt tttaaagaaa tcagtcattt tatcttcctc
     7201 cttcgatcac tttgatacga ctatctaaat cgtttttttg taaaatattt atttttacac
     7261 cagatttgat caaataatcc aatgcttttt tccgctgttt ggtttctcca gctaaagtaa
     7321 cgatcaaact ccctaacggc gtttcttgta ataactctac attgccatat aaaatattcg
     7381 ctgttacttg atatttgctg tacagatgag cgattagagg ttcgttcgtc tgatttccaa
     7441 tataacttaa ttccaccagc catttattgg cgatctgatc ggcaaaagca gaatgggcaa
     7501 tgatcgtttc taatgcttgg tcaagatgag tagctgttcg aataaaatct ttggtcaatt
     7561 cttcttcagg attgctgaag atctggatgc tgctaccttt ttcaacgatt cttccatctt
     7621 ccataaccgc taccttgttg cagatttctt tgacaacttg catctcatga gtgatcagga
     7681 cgattgttaa acctaactgg cgattcaatt tttttaacaa tgcaaggatc tgaagggtcg
     7741 ttttcggatc aagtgcactg gttgcttcat cacataataa taccttaggt tcattcgcca
     7801 aagctcgtgc gattgctact cgttgttttt gtccccccga cagttgttta ggataagcag
     7861 atgctttttc ttccaaaccg acaagttcca gtagttcatt cactttttga cgacgttcct
     7921 gtttcgattt tccagaatac tttaaagaaa aatcaacgtt gtcaaaaata gttcggcttt
     7981 ccattagatt gaaatgctga aagatcatac cgatggcctt tctttctttt cgcaattctt
     8041 tgggagagag ctgactcaga ttcgtctgat tgacaatgac ttctcctgct gttggtttct
     8101 gcagcaaatt catcacacga actaacgtac ttttccctgc acctgagtaa ccgacgattc
     8161 cataaacatc gcctttttca atcgttaaat ctacagaatc tactgcctgt atctcttttt
     8221 ctttttgttt gaaacataca gaaacatctc gcaattcaat cattaaaatt cctccttatc
     8281 taaaaaaagc gtcctcaaga aaatgaaaat gtcattttct tgaggacgct tttttgcgtg
     8341 ttaccacctc atgttcaagc tgtgttcaca cacaactcct cttcaagtac gatcatactc
     8401 tagcaagata tcggtcgcag ccgtcatcgc cttacatcta tgctcgggat gaattgctcg
     8461 atggccattt tccaatagtc gttccttgct tcttctcagc taccgaagct ctctgtcaaa
     8521 ggttcttcta tcgtacttac catttcaatg caaataatat tcaaaaaaaa agtcctttta
     8581 ctaacataaa ctatgtcagt aaaaggacga cgtatcgcgg taccaccttt tttcggaagt
     8641 ttccttccct caagcccaac atctcaaaaa gagagattgg cgttcgtttt aacggtcgaa
     8701 cagttccgaa tttgcctact tatcgacaaa ttattctcca aggccatttt cagcttttct
     8761 tacttgactc ttctcaccag ccagagcctc tctgcaaagt ttccaaactt actttccttt
     8821 tcaacgaaat cgatattttg ttgttgggat taatctacat gaggaaaact gaaaagtcaa
     8881 gtacgaaatt caaattattt tttctatatt tatttttctg tttttttatt taacatttca
     8941 ataacagttc gaagatcttt tccttccgca agaagtttca atttttcctt ttcttcgacg
     9001 gtataatttc ggacaaaact ggaatcaggt atcaaaacaa cgcctaatcc tgctcgtgaa
     9061 gcagctgttg ctcctactaa agaatcttcc actgcaaagg ctgaactagc ttctaatcct
     9121 gttcgttcca aagctgtcag ataaggaatc ggtgaaggct tgttctccga cacttcgtct
     9181 ccgaatgtaa acgtgtccaa gtaatcagct aatttaaagt gatccaagat atctgttgct
     9241 cttttcgtaa cagtggtcgt agcaatgcct agtttcatat gacgttcttt taagtctgcc
     9301 aatatttctt ttgcatatgg ttttaagtgg atggctccgc ttgtcagctg ttgataaaaa
     9361 tattcttcac gcttcgctcg gacttcctgt actttttcag ggctacctgc tgctttcact
     9421 aaaatatcaa aactttgttt ccagtttttt ccgccccaag cgtcaacgat ttcttttggg
     9481 atatggatat caaattgctg aaatccccaa agccagccat cacgatacgt tttttctgtg
     9541 tcaactaaca aaccatctaa atcaaatatt gcacctttca tctatacatt aatcctttcc
     9601 aaaacaatac acatagttta gcacgactgg tccaaaaaga acagaagcaa agaaccagcc
     9661 ttctcgttta taagagttct tggaatcgac ggatatagat tttcttcaca gctgtcacaa
     9721 gcgccagata agcgatgact gtagcaaata accataacca gtaattgcca ggaagtggca
     9781 tcaagcctaa ttcagcacca aaacttgtaa aaggaaggac agaaccgaca ataattccta
     9841 atgtagtgat aacaaacatg gcaaatgttg cgttgctttg gataaatggt actttcggtg
     9901 tacgcagtgc atggagcact aaggtttgtg tccatagtga ttcgacaaac catcctgcat
     9961 ggaataatgc gatgaaaatc actcgctgtg aactatccaa tgtatggaaa cttccgccca
    10021 ctacagcagg acaaataaca aaatacatca acaagtaagt agtaatatca aatatcgaac
    10081 ttgttggacc aaagtaaacc atgaattccc caatactaga tgcattccat ttttttggct
    10141 cgtccaaata ttctttatcc atccgatccc atggcaagga aatgcaggaa acatcataga
    10201 tcagattcaa aaacaaaagt tgtaaaggca gcattggtaa aaatggcagg aacgtacttg
    10261 caatcagcac ggaaaacata ttgccaaaat tagaacttgc tgtagccttg atatatttca
    10321 tgatattccc aaaagtttct cgtccagaaa gaaggcctct ttccaaaatc gtcaaatctt
    10381 tttctaaaag gatcacgtct gcagattctt tcgcaatatc cacagctgta tctacagaaa
    10441 taccgatatc agccactttc attgcaggag cgtcattgat accatctcct aaaaatccga
    10501 cagtatgacc atttttacgt aaagctgtcg tcaaacgtgc tttttgctga ggattcaatt
    10561 ttacgaatac ttcatggtct tcagctactt gtgctaattc atgatcactc atttccgaga
    10621 ttttctcacc ggtaaccaat tcattgatct ctagacctac ttgacggcac actgaacgtg
    10681 taactaattc gttgtcccca gtcagaactt ttacagctac accatgttct ttcagagctt
    10741 tcaacgcttg tttcgttgtt tcttttggtg gatcaaggaa agcaagataa ccaatcaaaa
    10801 ccatgtctga ctcatctttg actgaaaatt ctccaacgac gcttggattg gtcttttgtg
    10861 cgacaccaat cactcgtaag ccatcttcat taagtgactg tacttttgca agtacggctt
    10921 cttttcgttc ttttgttaat ggtgtaacag taccgccaag atcaataaat ttagaaatgt
    10981 tcagcatttc ttcaatcgcg ccttttgtga tcatttgtgt tttaccagct ggatcagcaa
    11041 ccacaacact catacgacga cgttcaaaat caaaaggaat ttcatccact ttttgataat
    11101 tgatcttatc tgtatctaaa gtttctgttg cttcatcaat gatagccaca tctaacaaat
    11161 ttttcagccc tgtttgatag tagctattta gatacgcatg gcgcaaaaca cgatcatctt
    11221 catttccttc acaatccata tgatattcaa gaatgatttt atcttgtgtc aatgtccccg
    11281 ttttatccgt acataacaca tcgattgcac caaagttctg gatggcattc aaatttttga
    11341 tcactgttcc ttttttggcc atcacacttg ctcctttgac caaattcgtc gtaacgatca
    11401 ttggaagcat ttcaggtgtt agaccaactg ctacagatag tccaaaaaga aaagcttcta
    11461 gccaatcccc ttttgtaagc ccattgatga cgataactgc tggagccatc aatgacataa
    11521 atttgatcag tagcatggat gttttatgga tgcctaattc aaagcttgat tgaattggtt
    11581 tttcagatag cgtttctgct acatgaccaa atagtgtttc atttccaaca gaaacaatga
    11641 ttccttccgc acttccactg atgacattac ttcccataaa gacaagattt tcgtaacttg
    11701 tttcgctttg ctgatcgtct tcacatactt tcgcatgctt ttctactgga tagctttccc
    11761 ctgtcaatgc tgcttgggaa atgaataaat cctttgcgga caataaacgc atatccgctg
    11821 gtatcatatc ccctgctgaa agccgaacaa gatctccaca gacgatttct tccataggaa
    11881 tctctgtgta ctttccatta cgttttacag ctgccgttac tttcacaagt gatttcagtt
    11941 tttcagccgc ttgattggaa cgtacggact gaatcaacgt cattgtcccg ctgacgataa
    12001 ccatagtgaa gataatggct acaccgaaaa gatctttatc tccaggagct gctaatacat
    12061 attccgtgat gaatgaaata attcctaatg cgatcaacac taatgtaaat ggcgtgatgt
    12121 atgctttcaa tatttctaca atgaatggtg tttttttgcc atatgaaatc gcattttcac
    12181 cgtaattttc tcttgaacgt tcgatttgtt cttctgtcaa tccatttgct gatgcatgga
    12241 atagttcaaa taattcttgt ttcgtttttc cagcaaaagc tgcatagatt ttttgtgtat
    12301 ctttcttttt ttccatctca atctctcctt tgtgtccaaa agacagctgt tctcgctgct
    12361 tttggtcttt agctattcat cggatcgata gagaaaaaac tgaaaggggc tgagaacaga
    12421 tagataatag ccctggcctt tcctctagat ccttgttttt cgaatgactg tcatccattt
    12481 gttctcatct cctttactaa gtaattttaa ttcatgactt ccaatcattt ttcgaatagt
    12541 tatttttcca attagaccac ctccttttta tttttcaccg atggggattt gcggcaacaa
    12601 aaaaacctct cgccaaatac tcagcgaaag gtttgaaaaa gcatattaaa aaaagcttct
    12661 cagatctcat cgttgagttt tgacactgta caacgtaaaa agcagatacc tgcttttagc
    12721 taccttatga aaagccttat ctcgacaatt cctgttctac ccattggcgt ctctcgacgt
    12781 ttttgggcag tagcctatgt ttgtatagga gcctcaccta acaaatctta ttcggttttc
    12841 cttcgttaat ataacaacta aaaaagagga tgtcaaatca attttccatg tttttcatac
    12901 tttttttaaa atacacacgt tatattcctg tctcacgcgg ttttatttgt ctcattttcc
    12961 acctcccgct ataatagaaa tatagtagga aacgaggtgc tgttatggaa agaacacttg
    13021 ttattatcaa gccagacggc gtgagacgcc atttagtcgg aagcatcatc caacgttttg
    13081 aagcaaaggg actggccatt gctgaaatga agtttgaaac tatgactcct gaattagcaa
    13141 aagaacatta tcagcacttg actgagcgca gtttttttga tgaactgatc gattacatga
    13201 cttccggtcc tgtcgtttat cttgtgctgg tcggcgagga agtgatcgat atcgtccgta
    13261 agatggttgg agcaacaaaa gctgccgatg ctgttccagg tacgatccga ggagactacg
    13321 cgctgccggg aactgaaaac atcattcacg cttctgattc ccgtgatgct gcggtaaaag
    13381 agatcgcccg ctttttcccg ccattcgata taaacaaacc gtctgaaaaa gcggcgaagt
    13441 gatacaaatt ctaaactgct ggtctagttg ctgggttcag caatggccag cagttttttc
    13501 tctaaggacc tccgttgatt ctttcctaac atatattcga gcactgtgaa gaattcttgg
    13561 tagtctttcg tcttttcttc ccctaaagca gcagaaatca atctttgata ataatctgcc
    13621 atcaatgttt catagatcac tgtacttctt tctttatgtg ataagctccg tttcatatct
    13681 tcattcacac tacgtgcctt tttcaaccga ccattttcta tataaaacga agtcaggttc
    13741 attaaaaaag taaagagata ctgctcatga ttgttcaact gtgctaatcg tcgcattctt
    13801 tttttgattt tcaaataatt gttctcaatg aaccgatcac tgaatatcca aggaaaatca
    13861 gctagaatag atagttcaaa ttgaccatag tctggtatct gcatcaagta ttcttcaatg
    13921 attcggcatt cataacgtaa aaattcaaat tctgtctcag atacttctgt tttttcgacc
    13981 atgtgaagat tcaaaaccaa ataatacaga tcttctgttt tcttaaaatg cctacggtta
    14041 ttgctggtct ctttataagg atcaaagctg tcttcttgta cccgatctag cattcgtctt
    14101 tttttcttta actttattgg ttcatccagc tggtttcgca tccattcata ttctgcaagt
    14161 gtcatgttca ttcgctctaa aaatgatctc aatgttacaa atgacatttt tttcactgaa
    14221 tttgtctcaa tcccgactag agaagtctta aaacagattc cttccgctaa ttgggactgt
    14281 gtcaacttct tgttgactcg aagtttcttg atcaattcac catcatacat aatagttctc
    14341 ctttttaatt taaatttttc cacattcaat aaatagatac gttaaaaagg aaaaatacac
    14401 gaaaaaaata aaaaaatgtt tttttcataa aaaataccaa ccttcaagca ttagaatgtt
    14461 gtctaactac tgaaggttgg tttgattgtt tattgattat ttttttgctg ttcaacgtct
    14521 gttcttgtta ctggatataa gttggttgtt gttttattac cattttttga aaagacactt
    14581 gtcgaatgtt cgcctttttg cttttcgatt gcttcattac gttccgcttc atttttatag
    14641 tcatattttt tcggatcaat cgtctcaaat ccgtttggca cataaaaccg caagagattt
    14701 tcctgattca gcttatctga aagacttaac gcagtcttga ctttttcctg atctttggag
    14761 atttcctcat cgattcctgc tgctttcgta tcgatgattt ctcctgtctg attattgtaa
    14821 gcttttcctc ctaggacggt gtatttgggt gtcacaaagt tcccatttcg aaaagcaact
    14881 acttgatcat gctgagaaga taataagtct gtaccaaaat gaagataatc tttatcatcg
    14941 attcccaata gatgtagtaa tgtcggcaaa acatcgattt ctccaccgta ttcatgattg
    15001 actgtccctt tagtataacc aggcatatgg atcatcaatg gcactcgttg catctgtgcg
    15061 ttgtcaaatt catcccatgt atctggatct ttaccaagag cgcttgctaa atctttatta
    15121 tctgtattcg agatcccaaa atgatccccg taaatcacaa agattgaatt ttggtaaata
    15181 ccagattttt tcaaatacgt aaaaaattgt tccaatgatt gatccagata atgtgccgtt
    15241 cgaacataat tatctactgt gctattgcca gtattcaaag atggaaagtc gaaattcttg
    15301 tcatccgtat aataaggcgt gtggttggtt acggaaagaa atttcgtgta aaacggctgt
    15361 tgtagatgct caagatattg ggcagattct ctgaataaat ctttatctaa tatcccataa
    15421 cctaacgttt cgtcggattc atcaaaataa gatcgatcaa agaaattgtc ataacctaag
    15481 tttttatata catgatctct attccaaaaa ctagctacat tcccatggaa aacagcgctt
    15541 gtatagcctt gcttttgtcc aaggatcgct ggtgcggctt gaaagacatt gtctgaccca
    15601 agttctgtga ataatgagcc ttgaggtaaa ccaaatgtgc cagtttccaa catattttca
    15661 gcatcacttg tctttccctg tcctacttca tgaaagaaat tgtcaaagct gatcgttgct
    15721 tgattctgga aaatactatt taaaaacggt gtgacttctt gtccgtctac cttcatatta
    15781 atcaaaaact gctggaaact ttctaaatgg agaacgatca catttttccc tttagcgatc
    15841 ccaaatgttt cgggatttgg ctctgcataa tgtttctttg tataatctaa aacttcatca
    15901 atcccattac tgcttgcatg cgcgcgaaca ctgctagtca ttcctgtttt gattccgtca
    15961 taaatagtat acgcatcgat tcctagatat ttgacaatat atgaacggtc aaatgttcgc
    16021 tgcagcaatt gtggacggtt ggcttcgctc agcgctagat ttcctgaaaa aataagccct
    16081 gaaagacagg taacagcgat agccatcggt ttgccgacag ggttgctttt gataggtatt
    16141 ttcttgacct tcaaccaaac cagtagccca atgaacacag cgatatccag ccagtagatg
    16201 acatcatggg gcttcatcaa agaaaaggag cttcctgaca atccttgcga cactttcgaa
    16261 aagcctaata cagatttgac tgtaatgaaa tctgtgaatt cccgataaaa aataatattc
    16321 aagtacaaca aaagcgtatt taaaatatca atgatcaaaa gaatagctaa cgctggcttt
    16381 ggctttttaa tatacaaaga aaggccaaaa aacaataagg tagtcgcaat tgggttgatc
    16441 cacaagataa agtactggat cgtaccttcc acacctaatg aaaaatctgc atagtaggca
    16501 atgatcgtct tcagccagaa aaagaaaaca gctgtcaaaa cgattgctaa acgattttgg
    16561 cacaattttt taagttgtgt cacgtatatc acttctctct attcacattt tttcttattt
    16621 tgctcctgaa aaatgaaaat ttcctaaaaa agttgttatc ttaagatttt tttattcttt
    16681 ctaaagatta ttccgtccac ttttcacagc ataaaaccgc tgcgatgtaa gcgattaaac
    16741 taatgaagag ataaaagtct tgtggtgacc gcttcactcc acggtattca aaagccagtt
    16801 tctcggtatt cgtacaaaaa aagaggctgt gacaaaattc ttgtcgcagc tccttatttc
    16861 ttgatgcagg acagcttttt cacaacttct tccttaaacg gtgttctcct gccaactcct
    16921 cggccacaag tcacaatatt tataaaacat gagaagctgt tttttgaaat tgttccttgt
    16981 ggctcagagt taaacggcag gctcaagacc tcttgataca cggtgttcaa tcagctgacc
    17041 ctcggtatta gattatgtca ggacgctaat tttacaacct cttggtaaac ggtgttcaaa
    17101 agcttgcatc tgcggtaata agcccaacca aacgaaaaat ataaggagct attttttgaa
    17161 attgctcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata cacggtgttc
    17221 aatcagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    17281 tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctgttt ttcattgtcc
    17341 ataggattga tattgtcctt gtgatgactc attcgtaata attgaggctt gattttcttc
    17401 acttcccgtt atcgtttgtc cactgccaga atctgaagga cttgttcctg tcgactcgtt
    17461 tttttgagaa gagacagttg ttttcttgac ttgttcattt gtctttttcc attccgctaa
    17521 acgatcggcg accacttttt tgtctacttt ttcagaataa gcggtgatca cttttccatt
    17581 tttcaaacgg gctactttgt cgccaacatg agtcaaaagc aaaacggtca aatgcttgtc
    17641 tttaaattga tattgtctga gagaaatcgc actaagtacg tcatcgatat taataaattg
    17701 attttcttct tttccttttg gcaaataaat gtccaattcg cctttttcgt aattggcagt
    17761 tattttatac tccgcccacc aataagttga gaattctttt ttgaaattat ctactgattc
    17821 tttgcttgct acgatttttt ctgtttcttt ctgagcaaga tctgctgtca tttttcctga
    17881 aaatgctgcg gataaaataa gcagtaagaa aaaaccgaat aaccaaaaga gaagaaacgg
    17941 tctttttttc ctcatgcttt cctccactcc tatttaattt tgactattct attatataca
    18001 aaaaatgaaa tagaaagaca aaaaagttga aaaagatcgt aaataattga acttcttttt
    18061 cacaaaatgg taagaatgga gaatttcacg catttgctct caactattct ttcttaagcc
    18121 tcaaatccgt ttttatctga tacgtctttg aaccaatgac ccgatttttt gattgttctt
    18181 tttccatctt gtgctaagtc tactgaaata aagccatatc gattcttata tgcattggtc
    18241 cacgaccagt tatccataca tgtccacata tgatagccta aacaattggc accttcttga
    18301 atggcttgat ggacatattt cagatgagat tggacaaatt cgatccgata atcatcgtgg
    18361 atcatgccat cttctttgat aaatcgtgct tcgttttcta cacccatacc gttttcagaa
    18421 ataaagcagt cgatatttcc ataatgatct cgtacattga tcaaaatatc atagattcct
    18481 tgttcataga tctcccatcc gcgataagga ttcattttac gaccaggcat ttcataattg
    18541 tcgaaataat cctcaggcat tggaccgttt ttagtatcga ttggtgtttc ttttgctttt
    18601 acccggcgtg gctggtaata attgatacct aaaagatcta ctgtattttc tttgataacc
    18661 gtcaaatcct ctgcatggat cacaggcatg agatcaagtt cttttactat ctcaatcaat
    18721 tcacttggaa actcgccttt tacagctgga tcaaggaatg aacgattgaa gaacgcatct
    18781 gctatttgcg ctgctttaac atcttcaggg tttgattcgt ctcttggata gcttggtgtc
    18841 agattcaaga tgataccaat aacaccacct tgctccattt catggtaaga tttgatggct
    18901 aacgctgaag ccaacgcttc atgatagcca acttggatcg cttttttcat atctgactct
    18961 tctggataat gccatcgata taaataacct gcttcgactg gaacgattgg ttcattatga
    19021 gtaaaccatt tttttacttt cgaaccaaac aattcaaaac aacttttggc atatttttca
    19081 taggcttcaa ccgtttctcg gttcaaccat ccgccttttt cttgcagata catcggcata
    19141 tcgaaatgat acagattcac aaagggttcg ataccgtttt caatcaactc atcaaaataa
    19201 cgatgataga aacgaacggc ttcttcgttg attgttccag attcatcagg tatcaaacgg
    19261 ctccattgga tagatgttcg aaatgaatta tgcccagtct gtttcatcaa ctgtacatcc
    19321 tcttgatact tcgtataaac ttgcgaggtt ttttcaggtc ctacttgatc aaagaacttt
    19381 tgcggttctt ttttgtacca ataatcaaaa atgttctctc cttttccatc gcctgaaatc
    19441 cttccttctg tttgtgggcc acttgctgca gaaccccacc aaaaatttgc tggaaattga
    19501 tattttttca tcaaagcacc tctttttctt ttttagttcg ttttagttga ttccatgtat
    19561 gatattttcc atcacaatca ctttttcatt ttcttcctct tcttttttga ttcgttttaa
    19621 cagctgttct gctgctcgtt ccccgatttt atctgttggt ggcgtgatcg tgattttcgg
    19681 aaggatactc agatcgtttt tatctaaata atcatagtga ctgaacgcaa tatccttccc
    19741 gattctgaca ccttcttgat tgaaacattc gatggcacca atcgacatat tgtaattgct
    19801 gacaaaaata gcagttgccc ctttttctaa taatgctttt gccccgtaat agccgctttt
    19861 tctagaatag tctcctgtaa agacctctaa ttggatcgat ggatcatttt ggaccgcttc
    19921 tttggctcct gctaaacgtt ctcttgctac gtagtctgtt tgagtggcag caataaatcc
    19981 gattttttta tgtccttgtt caccatgatg agcgatcact tttgctacac tttctcgatc
    20041 attcactaat attgtatcaa tacctgctgg tccttctgga caattcaatg cgataactgg
    20101 aatactcatt tttgtgagca tttcaaatcc agaccactct tcttcggata agaagacaat
    20161 cagcccatcg atcacatgct tcatcaggta ttcaattttt tgttcaattt tttctggctg
    20221 atcggcaaac ccgcttaata ataagctata cccattttct tcagctactc tttcgatccc
    20281 tgagacaact tccgacccaa aacggctgga aatcccgttt atcaaaagtc caatagagaa
    20341 gcttcgatta ttcttcaacc ctttagcaat gatgttttct ttatagtcca acgctgaaat
    20401 cgcttctgct atttttttca tgttcttttc cttcagttgc tgaccattca agtaacggga
    20461 aactgttccc actgaaacac cagccatctt agccacatca cggatcgatg ccatacattc
    20521 tcactcctgc cagttacatt tgatttctct ttcttgttgt tcgattatga tgaccgcatt
    20581 ctttgcatag tgttgcttgt catcaatatc catataacta aagtcttcag tagagcaact
    20641 tcctctcact attaattata cttaaacaag tatttgaacc ggttcatttt taatatagca
    20701 tttttttaaa agattgcaac acttttttaa aacgctttct gaaagtggcg gattttttgc
    20761 ctttttaaaa ttttattcta aaatttactc tatttgtggg tgttttgtgc gtttttctct
    20821 tccatccgtt tgaagtttta cattccaatg gtataatttc tttatcctat caaatggggg
    20881 aagtattttg aagaaattaa tcgtactatt tattttgtct gcgggacttt tgacattgac
    20941 tggctgtgat ttgtttggcg gctctgacca atcctcggac gaaacaaccg attcttctgt
    21001 cttaaaagcg gaaaaagcag ataccaccaa cagtgtatat acgctaaaag tacgtaaaga
    21061 tatggattta tccgcgggac agttgacatt tacagataaa aaaatggaat ggacacgcac
    21121 gtatcagaca caaggaactg attcttctga atcatctgaa gaaaaaacag tgctgacaga
    21181 tatcaagatc actaccaaag atgacgaata tattatttca ggaaaagaaa ataaaaaaga
    21241 aacaacagtc atatttacaa aaatcggtaa ttaccgtttg aaggatgagg atggcaacat
    21301 ctacagtcta tagtgccgtc tgttggatct tcttttgttt ctccccttct tttctgctat
    21361 agtaaagaag caagatgatt gtaaaggaga cgaaatggat gatcgatagt cctgattatt
    21421 gtacacgtaa taacaacaaa aatatccata cagaatttaa tgaccaagga agcttgagca
    21481 tatgcgacaa ctgtcacaaa ctagtcaaag attcatttaa aacatttgaa tcttatcctg
    21541 ttcaaaatta tgtaaaaagt agaagaagat aaacaaatcc aagcaaaact cactatggct
    21601 gatttttatg aacttcaaac tcagcttctt cctttgatca agcttcatca gtaaaacaaa
    21661 cagtggatca tttcagttta caaatcaaag agtaaagatt tatttatttc aaatatttga
    21721 atataaaata accaaacgat agaaaggctt attctacatc acgtagtaat ttttctttcc
    21781 cgtcgtttgg ttttttattt ttagcctaat tcttgacctt ttgcatgctg gttatccgta
    21841 aaatattttc cagcaggctt tccttccaat tcagctaatt tttcttggat ctgacggatc
    21901 accttttttg catcttctgg attttctaca aaatcgaatt tatccccatc cacttgtatt
    21961 ttaggacata catcaaaatc ttcataccat tgattatagc gggtgttcaa cgtttcataa
    22021 taggtatata acgtctcatc gctttctagc tgttcatagt cacgcccacg ttttttgata
    22081 cgttctagca tcgtgtccaa agaaactttg atatggacca tcaaatcagg ccgttttttt
    22141 ggagcaacat catctaactc gtttagcatc gtatctaata agctatcgta ctgctgtact
    22201 tcgatatccg ttacccgacc taaatcagcg tttagatgga acaacaggct atcttcatag
    22261 atggatcggt ctagcacatt gtcatcgttc actaaagcat ttttcattgc taaaaatcgc
    22321 ttgttcaaaa agaagatttg caataaaaaa gcgtactgct caggatttga ataaaagagc
    22381 ggcaatactt cgttatcttc gacattttca taaaatggtt tagaatcaat ctcttttgca
    22441 agcatatccg tcaaactgct cttacctgca ccaatcgttc ctgaaatcaa aatcactgac
    22501 atacaaccct tcctcttcta taagtactcg cctacgtaag tttcaacagg caatgtttca
    22561 aaatcatatt cacgtacagc ttggtctgat tttaaatacg tatggagact ttgtgtaatc
    22621 atcaagttcg taggtaccgt ctcttcttgt acaatgatca tcggtttttt caacatagtt
    22681 gccacaccta attcgtaagc cgttcctgga tcgattgttt gatgctcaaa gtcaaggata
    22741 gcaacaacaa attctgcatt tgtcagttct tccatatctt tttcgtatac ttcctgcgcc
    22801 catccagcgc tgaatagttc ataattgctt tcttggtgat gacgtggaga gtagaaactt
    22861 gtgactgttt tgttttcctc caacgctttt tcgatacgac ttactcgatc gatttgctcc
    22921 tctgaaaaaa atggtcctgc taaatatatt tgactcatta ttttgcctcc ttcaataata
    22981 actgattttt aatagatact gctcgtaatt cattgaccag ttccaatgga ttgttggaaa
    23041 agattacttg atccaaccat tttttatcca taaaatcctc ttttgcaaaa gtttctaata
    23101 attgttgcaa gccgctgtaa taaccgccaa tattcaatat tccgattggc ttgtcgggcg
    23161 tcagaccgat tgctatccaa gaaagaagtt gtgagaattc ctccaatgta cctagacctc
    23221 cagggaaaac gaggacagca tctccctttt caaataataa tacctttctt tcatcgatcg
    23281 tttctgtagg aataaatgta gtaacatctt ttccaggcaa ttcagtttca aaaagcccat
    23341 tcggatagat acctgtgact tttccgttat tttccagtac tgttttacta atcactccca
    23401 taatcccgga gtatgaacca ccgtaaacca gttcgatttc ttcttgtgcc atgtattttc
    23461 ctaacgtttg cgcaataaat ttgtaacttt ctttattacc aaaacgggaa ccgcaaaaaa
    23521 cagtaagttt cataaaaaaa cactccctaa ctcaaaagtc tatcttcatt ctacacgtgt
    23581 ttttttaaaa taaaagacta gattttgtag ttttttagtg gtatatagga tagacgccac
    23641 tatatatagt atttagaaaa aaatcatttt tcagccattt tatagataaa acaaaaataa
    23701 ataacgtgtt ttcatcgata tgaaatgact tacttataga agatactggg atttcgtcct
    23761 atatgccaat aacgtgtatt attttaaaca acaaatactt gtttctttta ttaacgaaaa
    23821 aatcattttg ttctcgtgtt atgtttcact gataaatgat gtttctctaa ataaaaaagc
    23881 cgacgaacta aacgatcaaa atttgatcat ttttgttcat cagcttctcc taaaaatctt
    23941 tttgttaacg ataaatagct ggatttgtaa aaatatcact tgaatcgtca ctgctggaag
    24001 aaaattcagt gataaccgca cctgttgcgc ctgcctggaa ctagtgtttg atattgggct
    24061 cgaacgtgta ctgatctcta gcatgaagaa taatttcttt ttttactgta taataacttt
    24121 ctttcctttt tggaatagta caggaaagtt tttttgtttc ctttccttcg acataaggta
    24181 taccagaccc tttcgacaac gaaaggttct catttttcct tcatcgtcat ttcaaggagg
    24241 gtgccgatgc tctggacagt ttcgatcgct cctaaaccaa aatctgtata ttcgattctt
    24301 tctaactctg attctttcat accattcctg tctcaaggta catctattgt acttttttct
    24361 tgtaatccat tctgcctctc ctaactaatt gtcaaatcat cccaagatat gtcttcttcc
    24421 tctttttctt cttggatgat ttctgcattc ggatctactg gttttttcaa taataaaatg
    24481 gttattcctg tgacaaaaga accagctaac aatgcaccaa tgaatgccca tggtctggtc
    24541 atcgtagcga ctgcaaacat tcccgatgcc ggaatacggc tatctgcgcc ccaaaacatg
    24601 cttaatccgc cacctactgt cccaccgact cccgttgcta tgacattgcg tgccaagtcg
    24661 ttcaatacga tagggaaaca tccctctgtg atctgacaga tccccatagg aaaagcggtc
    24721 ttcaatgtat cgatttcttg acgattgagt atatttttac gaatgatttt ccctaagaaa
    24781 taagcgatcg tcaagccgaa tggggttacc atcgaagcta aaatcaatac agtgattggt
    24841 tcatagattc cttctgaaaa tcgtgccaca cccccttatc cagaataaat ggtaatccag
    24901 aaacagccgt gtatctgcgg taataagcct aaccaaacga aaaatatgag aagctatttt
    24961 tgtttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct ttttttattt
    25021 atgtttcata atcaatatgg taaggatgca gctccgtatg gtagaccgcc tccgaatatt
    25081 caatcatttc cccaatttgc gtagacgatt tccgtatccg tttgatgccg atctgtgcgt
    25141 ctgttttcag aattccttgt tcttcttctg ttaaaaatac tgccgagaaa tcatcagaaa
    25201 atgagtcaat agtatatcct tttttcgtca gcaaacgata caaagattct ttgccaaatt
    25261 cttctctgct gaattgcatc atttcttgtg gaagataatg aacaaaataa atataaggct
    25321 gattgttcaa ataatataag cgcttaaaac aaacagcttg ctcaccgaaa aaggaaaaca
    25381 aaggatcttc cttctttata tggaccaatt gcaaatccag cgtttctttc cggacttcat
    25441 atcgggagtt ctccaagatt tgcgtaaagc ttgccgcttt ggataatcga ttgtaaggac
    25501 gattgcttag aaccgtcgtc ccacgaccac ttttctttgc gacatattca tctgatgcca
    25561 gcagttcgat tgcctttcgg acagtgattt tgctgacttg aaacagttct tctaattcac
    25621 tttctgtcgg caacatcgaa ccgactggat attttcctga gaaaatatct tctttaattt
    25681 tatctgcgac ttccaagtat agtacacgtg tccttttcat aacgtcaccc tttgctttac
    25741 tattactaaa agaatagcac ataaaaaata aaaaaagaac acaaatcaca tgtgagaagt
    25801 gtgttctttt tcattcagct gctttaatta caagtttacg ccatttgagg caattacttc
    25861 tttataccaa tcaaaagatt ttttctttga tcgattcagt gtcccatttc cttcatcgtc
    25921 tagatccaca tagataaagc cgtaacgttt actcatttct cctgttgatg cactgacaag
    25981 atcgatacat ccccaaggcg tatagcccat caaatctaca ccgtctgcta ctgcattagc
    26041 catcgcttcg atatgacgac gtaaataatc gatccgataa tcatcaataa tcgtgccatc
    26101 ctcttccata tgatcaatgg ctccaagacc gttttcaaca atgaataatg gtttctggta
    26161 acgattgtat aattcattca atgcgattct cagcccttct ggatcgatct gccagcccca
    26221 ttcacttgcc tcaagaaacg gattttttac tcctcccagc aagttccctt ctgaagttgc
    26281 tgcttgtgga tcagtctcat tgattgccgt agacatataa taactaaatc cgatgtagtc
    26341 cactggatat tcttttagca actggaagtc tttttccgtt tgctctaatt tttccggatc
    26401 gactccatat tttttatggg tacgcgctgt gtatgcaggg tattctccac gtacttggac
    26461 atccgtgcag aaaaaattga attcttggtt ttggatcatt gttgctgcct gattgactgg
    26521 attggcatcg atgctgtaag ttgttgcata gataatcatg cagccgattt ggatatccga
    26581 acgcaattcg tggccgattt ttacggcttt gctacttgca acgaattgat tatgccatgc
    26641 ttggaaaata ttttggtact ctcctgctcc attgcttttt actaatcctt gacttaaagc
    26701 tggaaagtga aaggccgaat tgatttcatt gaaagtcatc caatatttga ctttactgct
    26761 gtatctctct aatactgtct gagcaaatcg ttcatagaag tcgatcagtt ttcgattttt
    26821 ccagccaccg tattcttttg ccaaatgaag aggcatttcg taatgagaaa tcgtgatcac
    26881 aggttcaata tcatatttta agcactcatc aatcagctga tcataaaatt ctaatcccgc
    26941 ttcgttagga gtaccttcgt ctccatttgg aaaaattcgt gtccaggcaa tcgaaaaacg
    27001 ataacatttg aatcccattt ccgcaaacaa ggcaagatct tctttgaaac gatcgtaatg
    27061 atcgattcct cgatgattcg ggtaaatata tttttcttga tcaatcgtcc agtcaaattc
    27121 ttcactgccg ataatcgcaa agcgctgctt acctccaggc atagcatctg caacggatag
    27181 tccttttccg tctcgatcat aagccccttc taattgattt ggcagcagtt gctccacccc
    27241 ataaaaattt ttctgaaaaa cgtgattgca tcctgatttc ctccttattt tacaattgcc
    27301 agaacatctt cgtttgcaat aatctctttt tggtctaatt caagtatatc ggcaaatgca
    27361 tcagtattcg taatgacaac tggagtgatt acgctatagc cagctgctct aattgcttcg
    27421 atgtcaaaac gaatcaataa atctcctgct ttgacgagat ccccttgttt gactaagatc
    27481 tcaaagccat tcccatttaa ttctactgta tccatcccga tatgaatcaa aatctccgct
    27541 ccgtcagtcg ttgtgatccc tacagcatgt ccagttggaa aaatagtcgt gatctctccg
    27601 tctgcaggtg caactagttc tcctacttct ggatcaatag cgataccttt tcccatcacg
    27661 ccagaagaaa aaacttggtc tggtacttca ctcaatggca gtactttacc cgttaaagga
    27721 ctttttaagg tatgccgtgc tgaagtaatc ggttctcctg cattcgcatg tttgttttct
    27781 aattgattgt cttgtgtttg ttcgtcaaat cctaaaatca atgtacctac gaatgcaaga
    27841 acaaaagcaa ttcctgtagc tattactgct actgtcacat ttgactctac accatccacc
    27901 gtattaatga aagtcggaat cgtcaataag ctgaccaaac tgctggagaa agcttttact
    27961 ccggaaaatc cgataatagc tccaccaata cctccagaaa tacaagcggc aatgaatggt
    28021 ttcttcaaag gtaatgtcac accatacaca gttggttcag tgattccaaa taacgaagtc
    28081 actgtagaag aaatccctaa tgctcgaagt ttcgtatctt ttgttcgcaa tgctacagct
    28141 aaagcagccc caccttgtgc caagatggct ggaagaagca ttggcatcaa tacatcaaac
    28201 ccatattgtt cgatattcaa aaacatgatc ggtacgaatc cccaatgcat gccgaacatc
    28261 acgaatactt gccataaact tcccatgatt agacctgcta cgattggact aaatccataa
    28321 atcgagttga agagaccgcc taaagcatta ccggcaaccg tcccgattgg tcctaaagca
    28381 agaaatgtta atggaaccat cactactaaa acgacaagcg gaactaaaat catttgcaga
    28441 aattgcggaa tcactttttt tacgaatggc tctaatttac tttgaaccca tacggctaga
    28501 atgattggta tgacagaaga agtataacca gatggtgaaa gaatcactgg gatcccaaag
    28561 aaactgatag cttctcccgc accagcaact gctgtgatag ctggatacac aagtgccata
    28621 gcaattgcta ctgctaaaaa ctggttggct ttgaactttt tagctgctgt aaaagcaagc
    28681 atgacaggta agaaggtaaa gacaccatct gctgcagcaa acaatatttg ataagtaccg
    28741 ctatcagctg tcagccagcc catcaccgtt gccaatgata agaatccttt caataccccg
    28801 gcggctgcca tcgcccctag aaaaggtgta aatatactag agataatatc gatcagacga
    28861 ttcaaaatat ttttggactc ttcttttcca tcctccgaaa ctgcaagacc ttcctcatcc
    28921 acgatcgatt gataaacatc tgctacatta ctgccaatca ctacttgata ctgaccgcca
    28981 ctttgtacga cttggatcac gtccgggtct tcttttaact tttgcgtatc tgctaaagat
    29041 tcatctttta atctgaatcg caaccgcgta gcacaatgta ctaaactttt gacattcttt
    29101 tgtcctccta ccgcttccca tacacgacga ccaactgctt gattatccat tgttttttcc
    29161 ttctttcttt tttatttaaa aaataaaaac ccagattgaa aaaaacagtc acaaacattc
    29221 gtgaacctat tttttcaatc caggttttgc ctgccataca gtaacaatcc ttatgattgg
    29281 atgctttttt tctcacttac tactctttga atatgaatcg ttaaataaat ctgctctgcg
    29341 atccccattt cctgtttttg cgtttgtcga agatactcat tgatccgttc gacacaatga
    29401 aacgcttttg gatactttcc ttgaactaat gcgtagagaa attcatcttg ctcgtcagat
    29461 ccttcccctt tgagataacg ctgggcaaaa aactgcagat gagtaatgat acgctgataa
    29521 ttcaaagatt cttcatcgaa cacgatgccg aagaatttac tgatgatact cagtatatct
    29581 cttaccattg tggtagcggt catcgtcact tgcatgtctt cgtttccttg ttggctattc
    29641 acaaaatgaa aggcaataaa accggcttca tcctcagaaa gttccacacc cgtcttttgt
    29701 ttcacgaaca aaagagcctc tttcgctgct tgatattctt tcggataaaa tttttttgtt
    29761 tcaaataata atggattagg cagttggata ttttcttttg ctcgtgtgat cgcataatga
    29821 agatgatcgg tcaatgtcag ataaatatta ggcgaaaatt caacttttag tttatcttca
    29881 gccagttcga ctacttgttt gacccattcg atctcctctg ccggaatatc agccatcagc
    29941 tgctggaatt ggtttttagc aaaagaatct cttaaaacaa attctttttg gaccgcttct
    30001 ggcgaaatct cgtccccaat ctttttttga aaagctagtc cttttcccat acaaacgact
    30061 tcttccccga atccattttt cgaaatgacg acattgttat tcaaaatttt atcaattttc
    30121 attttgcttc gccctctcaa gaaaaataaa aaaacctaga attcgcctac tgtttcttag
    30181 ctgaattctg gttttgcctg ccgtacagta acaatccatt tcttattcaa aatcatcata
    30241 ccatcgaatt tttatttatg caagcctttt catataaaaa ataaaaaaac tggaacacaa
    30301 gtctatctag aaaacaacca aatcagagat gcacctcttt gcgtttctct ttttttcagc
    30361 aaattggttt ttccttatcg attcatatcc tttggctgtt tttaatctgt aacgacttat
    30421 gtcccagtat cctaaaatgt ttttgtctct agattgaaag cagatctcat atcatctgtc
    30481 gcatctttta tcgatgtaac actttccagt tctccttgta caactagtcg cgtgacggcg
    30541 tccatgtcgc cacaagtaat tagtgtgaca atttcttttc cctcgacttc attcagtaat
    30601 tgggtatctg taggagcaac tttttcttta aaaaatgttt tgtatgtata cacattttct
    30661 aaatctgtca aatagatctt ggcacccatc tctaaatttt ctagcggtgt aaacagcaat
    30721 tcagggttcg ttgcacgatg gcttgctaag gcataatttc cttttcccat ttcttgatct
    30781 ggagataatg ttcctgctcc gtaaagtagt gacgtattat ccaagccttt gaaaaccggt
    30841 aaattgattg aaactgaagg gactgcgata ctagcaatca ctggtagatc tgttcctctc
    30901 atttgcgacc tcatgacccc ttctgtcgtc attggttcta ccgcatcaaa gtcaaaactg
    30961 acatctttat cgttattctt tttcagatct tcttttgtta cattcgctat agcatattta
    31021 tctcctgtct ctctgacaaa ataatccttg atctgttcat tgaaaatcaa agctagtcct
    31081 actaataaca agaggaacaa gaggatattg actagccatc tttttgcttt tccttttttt
    31141 ttcattttct ctccctccag actcatttta ctgtttttct tttgctcttt ccaacagccg
    31201 aatgacacat ttctacaact ctgtgtattt ttttcgtaca cctgcgcttt tttctacagg
    31261 atatttcaaa ttatttttga tcaatttttc ttcaatgatc tcatctagat ccaggtgaag
    31321 gttgtctgcc atcatgtatg aatagatcaa tacatctgcc agttcttctt tgattcgttc
    31381 taattgtgtt tcttttactt cttccggcgt tttccattga aaaagttcta acaattcaga
    31441 tgcttccaaa gaaatagaga tagccaaatc tttttcgtta tgaaattgtc gccaattacg
    31501 ttcatctcta aactggttga ctttttccat actattcatt tgcttgcctt cttccaatca
    31561 cttcttactt gattaatcat accatttttt tacataaaaa atagatgatg cttttaaaaa
    31621 aactcctgtt ttcttttatt taaaataaat gtcatagaaa atcaataatt taggtagcca
    31681 ccatgtgtat gcagtagcgt gtcttcttcc cttgaggctt tttgtttttc gatacgttct
    31741 tcttcccaaa cagacaacgc tactagaatc tctcttaatg attttccttc ttcggtgagc
    31801 gagtactcta ctttaggcgg gacttggtta taggcttttc gatggatact ttcatcttgt
    31861 tctaactctc ttaactgctg ggtcagcatt ttttttgtga tcgtagggat ttgcttgctt
    31921 ctccacgcct aaaataagcg atattgctct tccctttatc tggtgtaagc aaagcagtta
    31981 gcattttgac gatcgtgggt tccgcttttc acttcttatc tacttagtat catgaagtat
    32041 actttcgtac taatactaag tagcggaagt gtcaactact ttgtttaact acatagcaaa
    32101 agaaacagaa gggtgacaac ggtcatcacg cttctgtttc ttttgaatat gctgtgttgt
    32161 tgtctctatc tccgtgtaga ctttcctcaa acttatcctt tgacagtaag tcagtaggct
    32221 gatttgctct ttctagatgc cagatacatt tctacaaaaa aatagtgatt ctgttttgaa
    32281 aattcgattt ttagttctgc attttcgtaa acatccaaca tttttttcgc actgtataga
    32341 cctaacccgc catttttttt agaggttttt cctcttttga ccatctccga tagcgtcaaa
    32401 ttcgttggat ttgccttgtt cgctatacta aaacgtgatc cctctttttc ttccagcaga
    32461 ttgatcgtaa tcggactctg atcggctaca cgatgatcga atgcgttaga aataaaaata
    32521 gataaacagc gaataaaaac aaccagatca atcgggattg ttttcacttc gacaggaata
    32581 actacttgga tcgggatttt ctccgattct gctttttcta gaaattttaa aaagacaccc
    32641 ctgacagctg gggatacaat atttgttagc tgttgtattt gcggtttttc aaaaatagct
    32701 gcagaatgat cttctaatat ttctaaaaat tccaacgttt cttttttatt ttcctgaaga
    32761 gtaattttta atgaaaacag tagactttta taatcatgtt tgaattcctc tagatgatgg
    32821 taatattctt catttatttg atacagttga agcgattcct gatgttgtct ttcttttact
    32881 tggaaactga tcaacataat ggaaaaaaac aaagaaaaaa aactgatgac tatcgacaaa
    32941 agagatagaa gcagataatt agatgagcgg aagtgaacac ttcccaatac caagtaaatc
    33001 acgccccagc ttaataaaat acaaatacct ccaaaatagt gttcagttgg ataagtaaaa
    33061 agcaagttga gaaatctcgt ctgtttgtct atacttttta ctaatagata gaaaataaat
    33121 gcgataagta gtgcaaaaaa acttgctgtt ccaagatgtc tctttcctaa ataatccatc
    33181 agatcaaaaa ccagaaaaaa gctgagtcca gtgatagcaa aaaaaagatt actgatcaaa
    33241 cagctgataa acaggttttt cgtagtaaaa taaaaaagaa acatcaataa taagccaact
    33301 aggtaaggaa agctcacttt agggatagga agtaaaagtg tcaccaccat caaagctata
    33361 caaccaaagc tgttttttac tagcagttgt ttcgaaaaga ataaatagat acctaaatct
    33421 aaaatcaaag taaaggtcaa ataaagtgtc actgttgcga ccatcttatt cctccattgt
    33481 tttgtttcaa aatgtacaca tattttttta ataggagcct gcatatgaaa aagtatttta
    33541 tcatagaaga taaccagttt cacagcgaat ggatcatcag agaattggtc aatcatcata
    33601 tcgagaatat cgtgcagctc acttctatct ctcaaatcaa tcagttgatc cagttgggca
    33661 ctattgataa tcacgatgtt ttctttatcg atatcgactt gaagcaatca ctcacaggta
    33721 ttgatgtcgc cgaacaaata cgtaaaaaaa atgcacatag taatattgtt tttttcaccg
    33781 cttatacgaa ttttgcagta gagatcatca atcggaaaat ccttccgatt ggatatatca
    33841 gaaaagacag gaatttatct gaacaaattc gtcaagtgct agaacagatc aattggaaag
    33901 aacagcagat cgtaaatgtc gataaaatct ttattgatca aaaaggtgga agtctcgtcc
    33961 tgtatcctcg ggaaatttta tatattgaaa gtatcaaagg tttgaaaaac caagttatgg
    34021 tcaaaacaac tacagaagat aaattagtga atacttcttt aaaacaattg aagacagagt
    34081 tagaaaagta tgcttacttt ttacttttaa aatcttactg tatcaactta tcgcaattgc
    34141 agaaaattga ttctgctcat tatgtgttag aatttttcaa cggtgactcc ttactcgttg
    34201 gtcgaaagat ctttgaaaag acaaaggaac gtttccatga cttccaaaaa actgcttcga
    34261 gttgatgaat aagagctgag acgtaagcat agcagatttc agggagtggg ccttgaatcg
    34321 gctccactcc ctgaaatcgt tgatatgatt ttacgcttcc tcttctgtag gagcatctgt
    34381 taacgaccat gtgaatgttg aaacgtagtt gtctacagca taattggata caggaacaaa
    34441 taattccact gattgtctcc cttgttccac attctctcct aatgaataaa tccatgtgcc
    34501 aatcccttca tttgctcctg cagttactag atctactgcc tgatctgctg ggatttcgat
    34561 tccttcagcg ccagttccag aaacggtaga tggcattcca ctgctattag ttgcgctatt
    34621 tcgaatcacg gcgtttttga atttcacctt tgaaccagtt agtttattcg tatcagatgt
    34681 tgtaccaata aattctgttc gtttgactga taatttccaa ccggcataat ttccgctttt
    34741 atccgttact tgtacgaaat tctgtacttg tgtttgtgct ccagtactgt ccgttccgct
    34801 ttgtaatgca gcatatactg tttttgctgt acctaacttg attgttccaa aatcaagatc
    34861 tggagcttgg tcaatcgtca gtaatccgga tgttccctga tttggttcgt caggtgtaaa
    34921 aggagtatca ggatcatttg gatcaacagg aggaactgga acaacgtctt caggatttgc
    34981 agcatctggt ttggtcacag aaatcgttcc ttctgtagtg gctgagctct tattctcagc
    35041 aaaaacactt tgtgtacata ataatagact tccagctaga acggtactta atgcaattgt
    35101 ttttttcatt tctttttcct tctttctttt aaaataaata atccagcaaa taagccaata
    35161 agaatgactc caacaagtat cagccatcgc cagtcaaatg tttctggctg attttttaaa
    35221 tcaatatcct tttcattcaa ttggtccgct gcatcatcag ttatttcaaa atcggtatgg
    35281 agattccagc gtttttcttt ccaagcaccc ttcacaaccg cttgataatg tcccggagct
    35341 aatttttttc cttctaatgg gacaagtaaa tcaaaatttg tattgggtgc tacatctatt
    35401 ttttcttttt ttattgaaag gtaaggctct tttgtttctt ttttgtagat ttcaaccgat
    35461 gtagtcaagc cgaacatata agaagcattt ggattttgaa gattcgcctt aatcacattt
    35521 cttccatcgc tttgatccgc ataagctttt tttaatttca aatctgggga agactctttt
    35581 tcgctatttc aaatcaagat tgctttcact tgagaaaact gattgacgac accaactgta
    35641 cttttgttag aaacgttctc ttttttttct tgaaacgtga tccctcctgc gaccacacca
    35701 gccagctctt tttctggcgc tttcagtgaa agtgtctcag tgaccgcact ttgaggagga
    35761 acagttattt ctcccactgt ttttgctaag tttttcatat cataacttaa tgtttcatca
    35821 aatttacttt ttgattctcc atattcgatc acaccgtttg aattcgtcgt agcggagtgg
    35881 atcaatgtct caactgttac tgatttatct gttcggttaa agaccgtgta ggggatttcc
    35941 tgtgttttcc caggctccat ccttacattt atatatgttt tgttcttatc aatctgatta
    36001 gccggtaatt caaaatctac cgcaaaattg aattcggagg catggacagg tgtctgccaa
    36061 aggatgaatc caaggagaag gatcataagt aaagagcagt tgtttcgatg acttttattc
    36121 catattcgat tcatcaatca tcctccttga tctttattct gtcggcgcat ctgctaatgt
    36181 ccatgttatc tctgctgagt aattaccagc tatctgattt tctttggtta agacaagttt
    36241 aaacgcccga ccgttattta aatattctga ataatctgct tgcacattcg ttgaattttg
    36301 tcctctgaca atttccagtt cttcttcatt cactaattga tactcctctc ctttcttgaa
    36361 atataggaca tcctgcatag aaaatcctgc atcatttgtg aaaggctgag ataattttat
    36421 cgttaagatc cacggatttt tcgtttctcg ctgatcataa atccataatt tttcctgtat
    36481 atccatttgg ccaaatgctt cttgtgtcaa accagttgct ttgatctgac caaaatcgag
    36541 tttggaaggg gcaagaaatg agagaccacc ttctactacg atcttcgtag caggtgcaaa
    36601 aggcggggca tcatgataaa caactggcgt atcacttgga ttgtgattcc caatttcatc
    36661 gttggtactt ggcttgttct gcacaccctt agcagtagct tctcctgctt ggtcgtttaa
    36721 tacaatttct aatacatctc ctgcaaaaag ttgattttct gggatagaaa gaacaaattc
    36781 ccctgtatca tcaatagttg tttctgacaa aacagcttga ttttttttca atgaaaccaa
    36841 ggctcctgct tcacctgtac ctgtgatcgt gcgagaagct gcattgatca atgtggtact
    36901 taaattagca ggttcaggag gaatcacatc gactgtcgtt ttggcagagt ccagttctgt
    36961 cacttcttgg ccacctctta cttttttagc agcaacaact tgcacttgat cctccgtttt
    37021 taatctctca tcattgggta cttgaacttc gaacaagccg ccttgttcgc cttctcccca
    37081 tgggttatat cctgcccctt cattttcatc tgtttgtcct tgcgtgagag cagtaatttc
    37141 tacagatgat ttcgtcgtat ctgcgtaagt aatacgtaaa gtcacttcta cttcgcctgt
    37201 ccaagcatct cttggctttg cctcgacacc ttcgggaaca acgacatgcc catagatttt
    37261 tttatctgca ttcgttggca ctcttaagga ttcaatcatc gggagctgat tattggcact
    37321 gattcgatta taatttttca ttccggcacc tgtctgagca aattcatttg ttaaacctgc
    37381 actagggctt gatacgaaat tgcctaagtc ggtcccattg atcgataagt ctgtcatatc
    37441 agaaaaataa tccgggtctt tttcaaagtt atcgaatgtt gcgcctgttt ttcgccataa
    37501 agcgatttct gaatgggcga tctttaaaga agaatctcca tttccttgga atacccagcc
    37561 gcctatgcca ttggtctcgc ttccaatccg tttgttttgg aaatcaaaat aggtaggaga
    37621 ttctaagtcc acattcaaca catccccact gatgatgccg gtcttatctt cccctgtttt
    37681 cccgaccatg actaaataag ttttttcacc tgccgtaaaa tttaaatcaa tatctccagt
    37741 tgtatctaca gttgctccgg aatctgcgcg tatatcgata tttgattttt ctccagtgac
    37801 tgtaaaatca gctgaacctg gactttctag aaaaccagaa gcgtcataaa attggatcgc
    37861 ttgatttcct tgattgagtc tgttcacgta agatttgcca tcacctgtat ttcttaccct
    37921 caatgagcct ccattggtta ctgtcagctt ttgcgtacca ttctcaaaac gaactgcgct
    37981 ggaacgccca ctctgcttat cgatgtcgat aactgatccg gctccttcta cgatcaaaga
    38041 aaggcctttt cctacaaaac gcatcccatt gttcccgggt tgatcgtcat ctccagccat
    38101 tttgaccgag atttgtccac cacttgatac atggatttga ctagaatctc cttgcaagcg
    38161 aaatccgccc gtagttgctg tattgatggt gattttccca ccattcgata cagtgacttt
    38221 cgatcctgaa cctctcattt ggatgattcc gcgattgttg tcattggtaa tattgatctt
    38281 tgtatttacc gaaaattcgc ttcccgaacc actgacttca aacatgacat taccaggtag
    38341 cgtatcgact gcctctcctt catcgctaac gccatctgcc cgatcataat caagagagac
    38401 ctttgaaccg cttaagatat ggattcctgg cgctgttttt ccactaccag tctcatctcc
    38461 tgaatctaga aaaccgtaga atttccctag atacggtcca taaagttgac taccattgat
    38521 cacattcaat tgatagcttc tacaaaaata ggctttaccc ggagcggcat tcagtccttc
    38581 cttcactggt tggacatcca acacaccgga aacaccatct aatgtgacag ttgcacgagg
    38641 agcgtaaatc agtcctaatt tggacccatc atacaaatca agtgtcccgg taaattttat
    38701 ttctcccgta aaatgatatc tgcttcccca aaaatcggtc atataagcaa tgataatggc
    38761 atttccatcg atatttggtg tcaatccacc tggatgtgca gctgttcctt taaaattctg
    38821 gaactcgatc gactgaggta tctcattgat ataaatatgg tgtgtgccga aattgtgagc
    38881 gaatccacca aaatcaataa cgatatctcg tttgatctct aattccctgt ctaaagtcaa
    38941 aggctgaatc agtcggatct tagttactga ttcgtctgcg agaagctgcg ccagttcttc
    39001 tgttgtggca gcttcgctgt acccatccgc tgaaacagct cttgccagtg ccgcttcact
    39061 gctatcagac gtttgttcag ctacttctga ggtctcactt gtcttatttt cttcttgcct
    39121 tttattagta gaagcctcac tttgagattc tgtagtttca gaagtaatcg attcttctga
    39181 aaaagtagtc ttgtcaaaac tttgattctc tgattgcgtt gattcgtttg ttttctctac
    39241 atcggcaaaa acggatgtca ccatcaagag agggaagaca aacaaaatcg acgcaatcag
    39301 cttttttaca cattgattca ttctccacca tcctctctga cgttttgaca attttatttt
    39361 atcgttttaa tctatttcca attgtacttt catatttaaa atatgtattt ttatggaaaa
    39421 atttttctat taggtgtaaa tttctttttt gtacaaaaaa aaggctgtga caaaagtctt
    39481 gtcacagtcc cttatcccga acaagcggtg ttctcccgcc aactcctcgg tcacaacctc
    39541 cattttaaca ataaacctgt aaatgtgagg gcaatacttt gattgtcaca ggtaatttat
    39601 ccccttcatc tccatcaaca ttcgtcgtca attcttcctc atcagctaat gacacaacta
    39661 attgctcaca agtaaggtag gctagatttt tcgtagattc atctactcct tttaataggt
    39721 ctggcaatgt tttaagcgta tcgatcaaag aagaatcttt taaataaacc aaatggagct
    39781 taccatcatc cacttttgca tttggaagta atgtttcaaa accgccgact gagttagtta
    39841 gtcctatcag caaggtactg ctctcaattt cttccttctt tccatcaact tctacgtgaa
    39901 acgagtagga ttgtgtggaa gctaactgtt tgattcctga catgaaataa gctagttttc
    39961 caaatttcgt cttcttctcc gcatctacat cattgattga ttcagggata gagccaattg
    40021 cgacgacatt cataaaatag tcgtcattta tttttccaat atccaatggc ttgactgatt
    40081 caatcgaaaa atgattgatc gcttcctcag gttctagcgg tatccctaat gctcgagcca
    40141 agtcattcac cgtacccaat gggaaaaatc cgaaatttgg ccgatgttct tgttcagcga
    40201 ttccactgat tccttcgttg actgttccat ctccgcccat gacaaacaca ctatgatacc
    40261 cttccgtagc cgcttcgcga gtaaagtttt ttgcatcccc tgcttttttt gtatgcaaga
    40321 cgaccacttc atcaaacacc gattccaatt ttgctatcgc taattgctca aattcctttg
    40381 cttgttcacc gcctgaactg gggtttacca ctaataaaac ttttttcatc actttctttc
    40441 ccttctgatt gatttcttta atataacgat agcataaaga aaaaaattag gctgtgatga
    40501 agtactgttc atgaaacatt catttatttt ttgacaagat aaaacatcca tactatgaaa
    40561 ctgaggaaaa ttaaaacaat gatataggaa ataaaagaaa atgataggca ttgtatacct
    40621 aataaagtaa aaatcttagg tggcagataa aaacacaaaa atagcaagag aatgtaatag
    40681 cgtttcttcc actttttgtt atcagacctt atagcgatca gaggaatacc aaatgcataa
    40741 ctgaaaaact cttgatgtac tgttaatatg tcacggcctt gaataataaa tgcgacaatc
    40801 cccgctataa ttagtgtgcc taacaccaaa acggaaaatt gatgaatata ttttatatac
    40861 aaatgatcca ttgtacttcc tgccttttta tttgtgagaa aaacaaatcc gtatacgtga
    40921 ataacttcta gaaataaaag aaatttgcaa aaccactcca tttatctact tctacgtgca
    40981 ttttttgata ttgttttctt tatttaagat t
//
LOCUS       DAFGZE010000021        40742 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.021, whole genome shotgun sequence.
ACCESSION   DAFGZE010000021 DAFGZE010000000
VERSION     DAFGZE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.021"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            116..1257
                     /locus_tag="LTU54_001139"
                     /pseudo
     CDS             116..1257
                     /locus_tag="LTU54_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(1383..2462)
                     /locus_tag="LTU54_001140"
     CDS             complement(1383..2462)
                     /locus_tag="LTU54_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL8295864.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(2565..3005)
                     /locus_tag="LTU54_001141"
     CDS             complement(2565..3005)
                     /locus_tag="LTU54_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8295865.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3062..3472)
                     /locus_tag="LTU54_001142"
     CDS             complement(3062..3472)
                     /locus_tag="LTU54_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL8295866.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(3652..4086)
                     /locus_tag="LTU54_001143"
     CDS             complement(3652..4086)
                     /locus_tag="LTU54_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8295867.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4186..5035)
                     /locus_tag="LTU54_001144"
                     /pseudo
     CDS             complement(4186..5035)
                     /locus_tag="LTU54_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
     gene            5424..7190
                     /locus_tag="LTU54_001145"
     CDS             5424..7190
                     /locus_tag="LTU54_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBL8295868.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            7292..8551
                     /locus_tag="LTU54_001146"
     CDS             7292..8551
                     /locus_tag="LTU54_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL8295869.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            8698..9999
                     /locus_tag="LTU54_001147"
     CDS             8698..9999
                     /locus_tag="LTU54_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295870.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10002..10853
                     /locus_tag="LTU54_001148"
     CDS             10002..10853
                     /locus_tag="LTU54_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8295871.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            10866..11681
                     /locus_tag="LTU54_001149"
     CDS             10866..11681
                     /locus_tag="LTU54_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HBL8295872.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            11832..12998
                     /locus_tag="LTU54_001150"
     CDS             11832..12998
                     /locus_tag="LTU54_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8295873.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13228..13875
                     /locus_tag="LTU54_001151"
     CDS             13228..13875
                     /locus_tag="LTU54_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBL8295874.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            13959..14903
                     /locus_tag="LTU54_001152"
     CDS             13959..14903
                     /locus_tag="LTU54_001152"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HBL8295875.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15038..16489
                     /locus_tag="LTU54_001153"
     CDS             15038..16489
                     /locus_tag="LTU54_001153"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL8295876.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            16510..17346
                     /locus_tag="LTU54_001154"
     CDS             16510..17346
                     /locus_tag="LTU54_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL8295877.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            17346..18005
                     /locus_tag="LTU54_001155"
     CDS             17346..18005
                     /locus_tag="LTU54_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL8295878.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18131..18820
                     /locus_tag="LTU54_001156"
     CDS             18131..18820
                     /locus_tag="LTU54_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295879.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            18826..19782
                     /locus_tag="LTU54_001157"
     CDS             18826..19782
                     /locus_tag="LTU54_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HBL8295880.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            19826..20338
                     /locus_tag="LTU54_001158"
     CDS             19826..20338
                     /locus_tag="LTU54_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295881.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(20594..21142)
                     /locus_tag="LTU54_001159"
     CDS             complement(20594..21142)
                     /locus_tag="LTU54_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295882.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21231..22412)
                     /locus_tag="LTU54_001160"
     CDS             complement(21231..22412)
                     /locus_tag="LTU54_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBL8295883.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(22648..24156)
                     /locus_tag="LTU54_001161"
     CDS             complement(22648..24156)
                     /locus_tag="LTU54_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBL8295884.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            24315..24506
                     /locus_tag="LTU54_001162"
     CDS             24315..24506
                     /locus_tag="LTU54_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295885.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            24709..25626
                     /locus_tag="LTU54_001163"
     CDS             24709..25626
                     /locus_tag="LTU54_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HBL8295886.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            25619..26692
                     /locus_tag="LTU54_001164"
     CDS             25619..26692
                     /locus_tag="LTU54_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295887.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            26840..27358
                     /locus_tag="LTU54_001165"
     CDS             26840..27358
                     /locus_tag="LTU54_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295888.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            27339..27731
                     /locus_tag="LTU54_001166"
     CDS             27339..27731
                     /locus_tag="LTU54_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HBL8295889.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            27807..28742
                     /locus_tag="LTU54_001167"
     CDS             27807..28742
                     /locus_tag="LTU54_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HBL8295890.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            28875..29654
                     /locus_tag="LTU54_001168"
     CDS             28875..29654
                     /locus_tag="LTU54_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBL8295891.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            29647..29964
                     /locus_tag="LTU54_001169"
     CDS             29647..29964
                     /locus_tag="LTU54_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBL8295892.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30208..31818
                     /locus_tag="LTU54_001170"
     CDS             30208..31818
                     /locus_tag="LTU54_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8295893.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            31975..32823
                     /locus_tag="LTU54_001171"
     CDS             31975..32823
                     /locus_tag="LTU54_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL8295894.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33054..33533
                     /locus_tag="LTU54_001172"
     CDS             33054..33533
                     /locus_tag="LTU54_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295895.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            33566..35794
                     /locus_tag="LTU54_001173"
     CDS             33566..35794
                     /locus_tag="LTU54_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295896.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            35923..37566
                     /locus_tag="LTU54_001174"
     CDS             35923..37566
                     /locus_tag="LTU54_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBL8295897.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(37639..38184)
                     /locus_tag="LTU54_001175"
     CDS             complement(37639..38184)
                     /locus_tag="LTU54_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HBL8295898.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38207..38692)
                     /locus_tag="LTU54_001176"
     CDS             complement(38207..38692)
                     /locus_tag="LTU54_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL8295899.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(38682..39494)
                     /locus_tag="LTU54_001177"
     CDS             complement(38682..39494)
                     /locus_tag="LTU54_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HBL8295900.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(39633..>40742)
                     /locus_tag="LTU54_001178"
     CDS             complement(39633..>40742)
                     /locus_tag="LTU54_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL8295901.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        13528 a         7387 c         7967 g        11860 t
ORIGIN      
        1 acttgaaaat attactgttt ttttcataaa tttagacaaa aaaacagtaa acatataggt
       61 agaatctcta ctatcttatt tcttgcatac aaaaaaatca aggagtgaga gagttatgag
      121 atattcaacc atcttaatat caacatttgc agccttaagc ttatcgctta ctataaatca
      181 gacagtccac acagaggaga cagaaacagc caaatggaca gaaaccaagt gggccaacgt
      241 caatcgaatt acctttcagg atgaattttc atccttcaat tcaggagtcg caaaattaag
      301 aaaaggacaa acagcgacca tcaaagcaaa aattgactat tcgggagact tagcacctat
      361 actacaatca gctgttacat ttgagaatgt tgatcccact ttgtcgattg gctatgacga
      421 gaacactttg actatccaac acaagcaagc gatatttacc ttcaccgtta cattaaatca
      481 ggacttgtta aaaccagcgc tttttactgt caaggtatca gatggactac cgaacgatca
      541 tcatttaacg ccgtatagtc agcggcagac aattgagcaa gattcagcca tcgactcagg
      601 aggagatctt gttgaagaac caacagataa accagaaaat gaaaataagc cagaagtacc
      661 gcctacagaa aatccagacg gagaacagaa accggaaata gaacctggag aagaacccga
      721 tacagaaact caaccagagc cagacaataa atcaaaacct gaaataaccc ctggagaaaa
      781 accagatgta actccagaac cagatacgga ttctggaaac caaacagtac cggaaacaaa
      841 tccagacaca gataatgaaa ctgaaaatcc agaaaaacca gaggtagatc cagaagaaaa
      901 accagatgtg actccagaac cagatacgga cgcaagagac caaggaatac cagaaaaaat
      961 aaacaaaaaa acaattcaag aggatggaaa aaaagagagt aaaaaatcaa atctagcaat
     1021 tctaaaaata aatgaagagc agctgaataa aaaatcacga atatttgata gcgcccaatc
     1081 agctgaaacc ttaaaaagta gcaaggatac aacctttgct gtcctgaaac gaaaaataaa
     1141 cagctaccaa aatccggaga gtcacaaaac aaagttatac tgtggagcgg aatcattcta
     1201 ttaagtatag caacaatgct ttctgcaaaa agatttaaac aaaacagatc tctctaacat
     1261 ttgaataatg tctgtgaacg catttttcaa ataaaaagaa atatccagaa aatgacccca
     1321 aaataccaga ttgaaatctg atatttgggg tcgttcatac tttatctttc ttcatattta
     1381 ttttaaggga tatctgtttc ttttggaaga tggttcggga tctttttcac tttcaataca
     1441 tctaaaaaga aggtaaagat cggaatgccg acaatcaagc cccaaacgcc aaagaaccgt
     1501 tcgctaacaa gtaagaccac aaatgtataa aaaatcggca gttctgtttt gctggacatc
     1561 aacttcggat tcagcacata agactcaatt agatggacaa tcaaaatcgt aatcaggata
     1621 taaacaacat ctcggatgcc tccttgggaa taagctatga aagatagagg aatacaagaa
     1681 atgataacac ctgctactgg gatcagactt aaaatgaaaa tcatgatcgc taaactcagc
     1741 agctgatgaa agccaaatac agccaaggcg attgtagtga gcaacgtatt taaaagagcg
     1801 acaacaaatt gagcttctaa taccaaacca aatgtattga caaacttatc ggcaaaatag
     1861 taaatatctt gaaagaacca agaaaattca ctttttaaaa acaatcttga gaatacaatt
     1921 gtttttttct tttcaatcat aaaaaagaaa cttaagatga atgacatagc aaacgccact
     1981 gccatttttc ctatatcctg taaatagcca agcgcaatcg aagcgccatt ttgaagctgt
     2041 tcaaaaaaat cgtttcgctc aatataagta tggatatatt gtaaaacagg atctgcatct
     2101 tttggctgat gctcgtaaaa attaataacg gactggatca tgtcaaatgt ttgatgaata
     2161 agcatcggga tatactttgt gaatgccaga tagataaggt aaacgatcag tgtataaatc
     2221 atgatagcta aaacacctgt agggattcgg acaaatcgct gaatgatttg aacaagatga
     2281 atcatcaaat aagtaaagat aaatgtgagc aaaatggttg taatcatact tctaactaag
     2341 aaaagaacaa aaacgacaaa taataaaaca gtgagacggc gtattttttc attcgccaaa
     2401 aaacgctgat aaacggtcaa atcgacaacc tccaaataac aaaaaataaa cacgttattc
     2461 attatatgat ggaatatgaa aataagaaag agcaaaaaga aaaaccgcta aatgacgtta
     2521 tcttatgagt tggttttcct cataaaatcg ttcatctagc agtttcagcc ctttttctgg
     2581 tcttgatgaa aattatctag catttttcgg gtcagatgtt gcagtgtatc caattcttcc
     2641 tgttcaagtg gaagataaga cttgatcttt tgtggaatac cttctgcttg tttttgcaac
     2701 gcagcacctt gagcagtcag cgtaacattt acgattcttt catcacccgt ttcacgctga
     2761 cgattaatat aatttttttc tgctaacttt tttagtaagg gagtcagcgt accagaatcc
     2821 agacacaaat catttcctag ctctttcact gaagtctttt tcttttccca taaaactaac
     2881 atcacaagat actgggtata ggtaagattc agcggcttga ggaggggagt gtagtgtcta
     2941 atgatttctt tagaggcggc atataaagga aaacatactt gattaactaa atatagatct
     3001 tccatttttc cacctccgct gttttactat agtgtatcat agcggattta cacaaaaaag
     3061 tctattcact aactgctttc agagtaactt ctatatttcc agctgtagct tttgaatacg
     3121 gacacacttt atgcgctgcc tctaataatt cttgcgtttc ttccagagaa agaccttcga
     3181 tagatccttc aatctctaca cctaagacaa agctcgtttc actttctgaa taaagggaaa
     3241 cttgtgcact gacagtagaa gccccagaga tatttttttg ctgtttgatc aattctaatg
     3301 cactgttaaa acatgcactg aaaccagctg caaacaattg ttcagggttg gttgcatttt
     3361 cgacgcgttt accaggagct gcaatattca attcgaatgt acgatcggga ttatgcactt
     3421 ctccagagcg tccgcctgtg ttgatcattt tcgtagaata catttttttc ataaaattac
     3481 cacctttaat tatattttat acaattgaat tttacacaat ataattgtgg aaagcaaaga
     3541 aatggattgg cataagataa ataagagaaa gcacatgaaa cagacgcgct ttctcgaata
     3601 gcaaacatat acaaaaaata tcagagacaa aaaataatct catcataaac attacatata
     3661 tttctcaaaa agttgttttc tttcttcaaa aattacttca cgatggacct gatgaagcat
     3721 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     3781 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     3841 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     3901 accaagagac gtgttttcat ccagcatatg gaatctcgta ccatacaagg taaaaatgac
     3961 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     4021 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatcgattt ccgttccttt
     4081 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     4141 gctaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     4201 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     4261 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     4321 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     4381 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     4441 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     4501 aatagataaa atagcataaa gaaactcttc tattccaaaa ataaacagat taagagaaac
     4561 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     4621 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     4681 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     4741 tgtttctgga actattgcaa tgattaaagg aaaaaccaag cttccgaaaa gtactttaaa
     4801 aaaagggttt tttacctaaa aatagaagag ctagtaccaa cataacgata ttcaacacta
     4861 aaataactat agctcggtca aagcctaaag cagcttccag taatattcct agaccgctga
     4921 cacctccagc agcaatatga tgaggcccta aaaacatatt gatagcgact cctaatatga
     4981 agagggcaaa aataatctta atgtatgtcc atataaattg tctcgtttct ttcatttatt
     5041 cacttcctta attcattaat aatactctta tcttattata acttactttt caccttaagt
     5101 taactttaat taataaaaaa attaggctag tctaaattat tatgtttcat tcttctagaa
     5161 tatacttcta cgccaaaaac tgttaaaaaa cttttacttt agaaaattgg agaaattgat
     5221 cgcttaagat aaattcagga cactaaatag tacaggaagt agactaaaac ttttaaaagt
     5281 tttaagtcta tctatttggc aagtttaaaa aataaaaatt cggtatcaaa aaactgttac
     5341 cgataacatt tttgaaagcg ttgtcttttg cttctctatc acttatagtg atgacgtcat
     5401 attaaaacac aaggaggaaa tcaatggata cagctgcaat ttatcatcga ccggaaagtg
     5461 aatatgcatt tctatatacg aatacaagat ttcgtattcg gcttcgtact aggaaaaatg
     5521 atatcaaaaa agtatatgtg ctatgtggag atccatatac cataacaact gaaaaatggt
     5581 atcaaaaaca acgaccaatg aaaaaatgtc taagtaccaa tgttcatgat tattgggaaa
     5641 ttgaagtgac gagcgaaaca agtcgcttac agtatgcgtt ccatgttgtt ggggaagata
     5701 acacggactg tttttacggt gatcaaggaa tttttcctta tcgagaaaat gtgattactg
     5761 aaccaaattt ttattttcga ataccttatt ttcatcaaat cgatcgattc actattccag
     5821 aatgggtaaa agaaacagta tggtatcaaa tttttcccga aagatttgca aacggggaca
     5881 aagcaaatga ccccgaacac actttacctt ggggaagtaa agatccagac cgagaagact
     5941 ttttcggggg agatttgcaa ggtgtgatgg atcatttgga ttacttgacg gaccttggca
     6001 tcaatggcat ctatttttgt cccatattta aagccacatc caatcataaa tacgatacga
     6061 ttgattatta tgaggtggat cccgcttttg gcgataaaaa acttttaaaa aacttgattg
     6121 atgaagcaca taaaagaggg atacgtatca tgttcgatgc tgtattcaat catatgggtg
     6181 tccattcacc acagtggcaa gacgttctca aaaacggaga aaaatcgatc tataaagact
     6241 ggttccacat ccgttttttc ccagtagatt cttatcagat gactgactta ccagaaactg
     6301 ctgaaaatat cccatatgat acttttgctt ttactccatt catgccgaaa ctaaatacag
     6361 caaatccaga agtgcaaaaa tatttgctgg atatcgcaac ttattggata aaagaattcg
     6421 atattgatgg atggcgtctt gatgttgcta atgaagtcga tcatcatttt tggaaaaaat
     6481 tcagagaagc agttacagaa atcaaaccag acatctatat ccttggagag atttggcatt
     6541 cctctcaagc ttggctgcaa ggggatgaat tccatgcagt gatgaattat gcatttacag
     6601 attcgattaa agattatttt gcaaaaaaga aaatcacagc ttcacaaatg gtcagtggaa
     6661 tgaatcatca gcaaatgctt tacagagatc aggtgaacga aggaacattc aacttattag
     6721 attctcatga tactgccagg atcttgaccc tttgccaagg taacaaagaa ttgatgaagt
     6781 ctgtgatggc ctttatgttt cttcaaaaag ggtctccttg tatctattac gggacagaaa
     6841 tagggatgac tggtgaagat gacccggatt gccgcaaatg tatgatatgg gagaaggagg
     6901 aacaagatct agagctattc ggttttataa aagaacttgt ttctctaagg aaacagttgt
     6961 ctaagattat ttctgaaggt tctactcagt ggctaatagt aaatgatcgt gaagataagc
     7021 tctatttcac acggaaacta gagggacaga tcatctatgt ttactttaat cagtcaaaag
     7081 aaccttgggt agtagaacag gaaaatgagg tgattcttag tcaaaattgt cagttgttag
     7141 aagacgggaa agcagagata cgacaatacg gatttttgat catgtgttga aaactgttac
     7201 cggtaacaac aagaaagcgt ttgcaaatga agatttgtgc ccattataat aagggtgtaa
     7261 cagcacgttg aatacaaagg aggaaatgaa gatgaaaaaa agaagtgtga aacagttggg
     7321 tttagggtta atctctttag gaatcttagg cggattagcg gcttgtggca atggtaactc
     7381 ttcagcagaa ggggaagccg atgaaggaaa aacgttaaca gtttccgttg attctggata
     7441 taaagattat ataaacgaaa taaaaaacac tttcgaaaaa gaaaacgatg tgaaaatcaa
     7501 gctagtagaa aaggacatgt tcgatcagtt ggaatcatta gcgttagatg ggcctgctgg
     7561 aaagggacct gacgttatga tggcagcata tgaccggatc ggtgcgttag gacagcaagg
     7621 acatctggcg gaagtaaaac tgggaaatga agcagcttac gatgaaacag acaaagccca
     7681 agtgacttat gatggaaaaa tctacggaga acctgcagta atcgagaccc ttgttttgta
     7741 ctataacaaa gatttagtcg atacagcacc atcaacgttt aaagatttgg agaatctatc
     7801 caaagacagt cgttttgctt ttgagtcaga agcaggaaaa aatacaggat ttttagctaa
     7861 atggactgac ttttattatt cctatggttt aattgcaggt tacggtggct atgtatttgg
     7921 tgatgatggc acaaatcctt cagatatcgg tttgaataat gcaggtgctg tagaaggaat
     7981 ctcttatgca acagattggt tcaaaaatgt ttggccaaag ggaatgcaag acataaaaag
     8041 tgcaggagac ttcgccagcc agcaattcat gtccaataaa actgcagcaa tcattgacgg
     8101 tccatggcaa gcgcaaacat acaaagaaaa caatatcaat tatggtgtag ccaaaattcc
     8161 tactctaaat aatggacagc cttatcaacc gtttggcgga ggaaaaggat gggtagtcag
     8221 caattatgct aaaaacaaag acctctctca aaaatggctt gattatgtaa cgaatcagga
     8281 aaatcaagaa aaattctttg aaatggtaaa tgaaatccct gcaaaccaac aagctaggga
     8341 aacagcaaag gctaaaaacg atgaattaac aacagctgtg attgaacaat atgaaacagc
     8401 tcaagcgatg ccaaacatcc cagaaatggg cgaagtttgg acaggtgcag aaaacttgat
     8461 gttcgatgca gcatcaggtt caaaaacacc aaaagaatcc gcagatgaag cagtaaaaac
     8521 aatcagtgaa gccatagaac aaaaatactg attaaaatga taaccgtcca tttttataaa
     8581 aaatggaaca ggtgcttcag ataaaagtgg caagagcgag atggaaaatt atcttgctct
     8641 tgttgccatt atcttaaaag cagtttgcgg tttagcaagt ttagaggggg cattcagatg
     8701 cagcaagata aaacaagcaa tatcaacgaa aaaaaaatca cgctttgttc acttattcca
     8761 gggatggggc agtttgtaaa tggccaagct tttaaaggta tcgttttttt cgccatattc
     8821 gtcctattca ttggtcagat gcttctgggc ggatttcatg cattggtagg attggttact
     8881 ttgggaagta taccaatgga agatcattca ttatttattt tgattgaagg cactttgcaa
     8941 ttcatcatta caatcatttt cttgattttt tacggtttga atcttttaga cgcttatcga
     9001 gtagcgaaga tgcgtcaaga gggcaaaaaa atcaatcgga cgatgaagga agtcatacat
     9061 aatataggtg atgaaggttt tccttatcta ttgacacttc ccgcttatct tctcatgatt
     9121 tttacgatca ttttcccagt cctagttaca ttgttcactg catttactaa ttatgacttc
     9181 aatcatattc caccagccag tttaattgat tgggtagggg ctaaaaactt ttttagtatc
     9241 ttctttttaa gctcatatcg taatactttt tttgcagtct tttcgtggac cgtgatttgg
     9301 actttatgtg caacaacttt gcaaatcacc cttggtattc tcacagcggt tgttgctcac
     9361 cagaaattta ttaaattcaa acgtattttc ggtatcattt tcttattacc atgggcggtc
     9421 ccagctttta ttactatcat gagtttttcg aatatattca acgatagcgt aggtgccatc
     9481 aatactcaag tgattccttt cataaaccag cttccttttg tagatatcag tgcaatctct
     9541 tggaaaacgg acccgaactg gacgaaagta gcaattatct tagtgcaggg ctggttagga
     9601 tttccctata tctatgtgat ggttacagga atcttacaat cgatttcaga agacttatat
     9661 gaagcagcaa aaatcgacgg agcaaatgca tggcagcgct tttcaaacat cacgcttcct
     9721 gctgtcttcc tagtggcagc gccaacattt attacacagt atacggggaa tttcaataat
     9781 ttctccatga tctatctgtt taacaatggc ggtcccggaa gcgtaggcgg aggagctggt
     9841 tctactgata ttcttatttc ctggatctat aaacttacga ctggaacttc tccgcagtat
     9901 tctatggctt cagcaatcac gttgattatc tccgtcatcg ttatctgtgt gtcattgctg
     9961 gtatttaaaa agacaaatgc gttcaatatg ggggattaac aatgaaaaaa tttcattcga
    10021 gtgctcgatt ccaaaaacga ctttctcact tttttactta cttttttctt ttaatactgt
    10081 cagtcattat catctatccg cttttgatca cagcaagtac tgccttcaag tcaggaaata
    10141 ttgcagcttt tagtttagat ctttcaagtg actggacttt aggaaacttt actcggctat
    10201 tcagtgaaac actttacggg acttggtata aaaatacatt gatcatttct gtcctaacaa
    10261 tgatcgtaca agtgactgta gtaactttgg cgggctatac ttatagtcgc tatcgtttta
    10321 tcggccgcaa atcaagtttg atgttctttt tagtgattca gatggtaccg actatggcag
    10381 cattgactgc cttttatgtc atggctttct tattagaagc gctcgataag tactggttct
    10441 tgacaatgat ctatataggc ggtggaattc caatgaatac ttggctgatg aaaggatact
    10501 ttgacactgt accaatagat ttagatgaat ctgctaaact tgatggggca gggcatttca
    10561 ggattttttt ccagattgta ttaccgcttg tcaaaccgat gctcgccgtt caagcattat
    10621 gggcttttat gggacctttt ggggatttca tgttagctaa gttcctgctt cgaacacctg
    10681 aaaatcagac tgttgctgtc ggcttacaaa catttatttc tgatgtgaca aaccaaagag
    10741 tctctctttt tgctgctggg gcaattttga ttgcactacc gatttgtcta ttattcttct
    10801 ttttacaaaa gaactttgtt accggcctga catctggcgg aacaaaagga taggagcaga
    10861 gtatcatgca aacatttccg ttaaactatt tttccgcatt acgtacgcca actcaagtgt
    10921 ttgcgggacg caaactatta tcatggccta aatttttctt gatatttgta tttttagttt
    10981 ccttgatggt catgcctgtg actctttttt atgccaatca gatccaagcg attccattag
    11041 aacaatttct atccgttcat tcgctgatcg atgaacaagg aacccaaaaa ttttctgaac
    11101 ttgaattatc tgaaactggg ttacaagcat cgcaacaaac gatagctgtg acccctgaaa
    11161 ttttggtagg tgtgtcttta tctgagaaac aacaatccga tcatggaact tttattgatt
    11221 ttgagaagga acaatgggtg atccaacaaa aggataaatc tggtatacgc cgctatacga
    11281 tgaattatag cccatctttc caaccagatt ctgtacgtac gcccgaagat ttccaacgat
    11341 ttttagaaag agaattttat gccagtaatc gtccaacaat cattttatct tatagtttaa
    11401 gtcttggtct tgtcctattt gtcatgacga gtctcatttt attcggggct tcctttttct
    11461 tgtggatgac aagaaaaagc caactttcat ccattcatac attcaaagag agtgcaaatt
    11521 tgatgctgaa tgttatggga ataggttcca tggcagcagc agttgtaggg tttatccact
    11581 ttgactttat cttgatgcta agtgtccaaa caacgataac tgtcttgctg cttttatggg
    11641 tttttgtcaa aacaagattc aaagataagc gtgtcgaatg acaagtttaa gcgatttatg
    11701 cgacaatgaa tgtgaaaaaa atgaaaaata aagtagtttt tgaaaaattt ctgaaaaaat
    11761 taaacttctt agttgattct tttctttttt agtattaacg taatagggta gtttttttaa
    11821 ggaggaaaat catgttagag aaaactttat acaatcaatt attttcacgt tctttctcgc
    11881 tccctgtcga agtaaattat tgggacggta caacgaaaca atacggagat acggacactc
    11941 ctccccaaat caaaatcaat atccatgaag aaatcccaat gaaagagatt acaagtaacg
    12001 catctttggc actaggagaa gcctatatgg atcatcgaat cgagatcgag ggaagtatcc
    12061 aagcattgat caacgatgct tatcagcaag caaacagctt tttacgagga aaagattatc
    12121 taaaatggct gccaaagaaa gaaaaacata cgaaacaaaa aaacaaagaa gatatccatg
    12181 cgcattatga cttaggtaac gacttttata aactttggct agatccaact ttgacttatt
    12241 cttgtgctta tttcaataca cctgaagata cgttggaaca agcacaaatc aataaagtcc
    12301 atcatatctt agacaaactc tttatcaaag aaggcgaaac gttgcttgat atcggttgtg
    12361 gctggggaac attgatgttc actgctacca aagagtataa tgtcaaggca actgggatca
    12421 cactaagtga agaacaatac gatttgatta ccaaaagaat aaaagaagaa catttagaag
    12481 ataaatgccg tgtattatta atggattacc gcgaactaaa aggcgaaacg tttgatcata
    12541 tcacaagtgt aggtatgttc gaacatgtag gttctgaaaa tcttgaaggt tatttcaaag
    12601 tggtaaaaga tttattgaaa ccaaaaggaa cagctttgat ccatggaatc agccgacaac
    12661 aaggcggtgc gacaaatgct tggatcaata aatatatttt ccctggcggt tatgtccctg
    12721 gtgttactga attagttgat aatattacaa aaaacgattt gcaactgatt gatttggaaa
    12781 gcttacgtcg tgactaccaa ttgacattag aacattggac aaagaatttt catcaagtcc
    12841 aagatcaagt cacgaaagaa atgggtgatt ccttctatcg aatgtgggat ctatatttac
    12901 aagcttgtgc agcttcattt gaatcaagta atatcgatgt gattcaatat ttgcttgttc
    12961 aacctggaaa caatgacatc ccaatgcatc gtgcataatt gaggcaaaag tactgtttaa
    13021 agactattta aaaagaagct aagttttatg atgctccaga agtgaacttt tttgtctaac
    13081 ttctaggatt gtctctaaag cttagtttct ttttctttat attttcataa taaaagtttg
    13141 gttctttaat ttttaaacat gacagaaaat tttttcttcg ttatacttgt aatagaatac
    13201 tattttataa caaaggggat cgaatcaatg aaaaaaactg ttatttatac tagtttattt
    13261 gcagctttgt ttttagctgc ttgtcaatca aataccaatc aaggcacatc tgggaatacg
    13321 tcttccagta gcgaacagcg ctcttcacag gtatcggact cctcaacaaa agcaagcaca
    13381 tcttcgtcta gccagacaaa aagttctagt gaagcaagta ctcagtcaga tatcaaagtg
    13441 tctgcagctc gtgcgatcga attgttccaa gataaatacc cagatactgc aattactagt
    13501 ttggaattag atagtgattg gggatcttac ttctacaaga tagaaggtgt cgatgatcaa
    13561 aaagaatacg aagcaagaat tgatgctcaa aatgaaaaaa tggagacaga gaatccagaa
    13621 actctagatc gagatgaaca aaatggtcaa aagaaaaaag aagatggtct ggatgttgat
    13681 aaattgatca cgattgagga agcagcaaaa atcgccgtcg acaaagtagg aagcggtacg
    13741 gcaacagatt gggatttgga taaagattta ggtactactt attgggaagt caaagtaaaa
    13801 gacggcaatc aaagtacatc agtcaagatc aatagccaaa caggcgaagt actgtcaact
    13861 gaacaagatg attaagaatt tcttcggaaa acaagagttg atttttcttt tagtcatgat
    13921 acactagaca aaataacttg tttgaaggag cgatttactt gagtttagaa acgacctttg
    13981 ccaatcatat ctttgccaat cctttgatga atgcatctgg tgtccactgc atgactaccc
    14041 aggaattaga tgaactggct cattcagaag caggagcatt tatcactaag agctgtacga
    14101 tcaatgaacg aaaaggaaat cccgaaccac gctattttga tgttccttta gggagtatca
    14161 attctatggg acttccaaac ttaggttttt cttattatct agagtatgct cttgcgtatg
    14221 aaaaagtgca ggaaaaccaa aatcaaccgt tatttttttc cattgccggg atgagtgtcc
    14281 aggaaaactt ggaaatgctt gagaaaatag aaaaaagtgg gtttaatggg atcactgaat
    14341 taaatttatc ttgtcccaat gtgcctggaa aaccacaact agcttacgat tttgaggcca
    14401 cttatgaaac actgaaagaa gtattcagca tcttttctaa gccgctagga atcaaattgc
    14461 ctccttattt tgattttgct cattttgatc agatggctga tatcttgaat cagtttccat
    14521 tgacctatgt caatgctatc aacagtgtgg gcaatggttt gtatatcgat acagaacaag
    14581 aagcagtagt catcaaacca aaagaagggt tcggcggcat tggcggagag tatatcaagc
    14641 cgactgcttt ggcgaatgtt cgcgcttttt atacacgttt gaaaccagaa atccaaatca
    14701 ttggtacagg tgggattcgg acaggacaag atgcatttga acatctgcta tgtggcgcaa
    14761 gcatgttaca gattggtacc gagcttcata aagaagggcc ggaaattttt tctagaatca
    14821 taaaagaatt aacgcagatt atgtcagaaa aaggatatac gtcaattgac gaatttaaag
    14881 gcaaattacg caccatttca tagcaaagag acgaaggtca tgattgatct tcgtcttttt
    14941 ttataaaaaa cactcgtctg ttcattcctg tgcaagcgta ctcattgtat gttagacgaa
    15001 cttgtgggaa aataacagag gcaaaaagga ggataatatg aatgatgcaa cagaattagc
    15061 aaaagagctt aaagaaaaag aatttagttt tcaagaacta gttacggata tacagaaaaa
    15121 aataaagaag caaaatcatg aattgaatgc gtttgtcgcg cttgaaccat tcgagagtaa
    15181 ttcccacgag ctattgattc cccagtcaga aaaagggcca ttggctggta tcccatttcc
    15241 tttaaaaatg ctcggtcaag aaaaaaaggg atggctagct acttcaggat ctcgtttatt
    15301 tgaaacacac cgagcttctc atacatctaa ttatgtgcgg caagcagaag cgataggtct
    15361 agttcctttt ggacaaacca atgcgccaga gtttggtttc aaaaatatta cagacccagt
    15421 catctatggc ccagcaagaa acccttggaa ccttgaatgt acaccgggcg gatcaagtgg
    15481 aggagctgct gctgcagttt catcaggaat cgttccttta gctggtgcta gtgatggcgg
    15541 tggttctatt agaatacccg cttcgttttg cggattgatt gggctgaaac cttcgcgagg
    15601 gacgatgcca gttggtccat atgcttggag aggatggcaa ggagctgcta ttgatttcgg
    15661 cttgacagtt tctatgagag acacggaggc tttattttat gggatgagaa gtatccattc
    15721 cggtgcgcca tatcaagtat cgccagttga gtggcaaaca catccaagaa aaaaacggtt
    15781 gaagatagct ttttgtgttg attcaccgat ccactcacag attagtgaag aggcagtact
    15841 tgctgtaaaa aaagctgtag atttccttgg aaaagaaggg catgatttga cagagattcc
    15901 ttacccacta gatggtcgta aactgatcac gtcttattat cagatgaatg gcgcagaaac
    15961 agcagcaatg atccgctcga ttgaaacagg catccagcga gctgtcagaa aagaagaact
    16021 tgaacccatt tcttggactt tgttccaata tggtcagaag ctctctgccg ctacttacat
    16081 cgattctctg catgtatggg atgaagcagc aattgtcatg gaaaacctac atcaagaata
    16141 cgatctattc ctttcaccaa cgactgcatt taccgcccct aaaatcagcg aagatttgca
    16201 aagtgatgcg ataagagcac agatggaaca catcagtgat tgttctgaag tagaaggttt
    16261 agacgttgtt tcttccatgt ttgaggagag cttgaagatc acaccttata cacagttggc
    16321 taatctgact ggacaaccag caatcagctt gcctactcat ttgacaaaag aaaacatgcc
    16381 tctaggaatc caatttatgg ctgccagagg aagagaagat ttgctatttc aaatcggcta
    16441 tctatttgaa gcaaatcatc agttccattt accaaatcca tggaaatagc agaaacaagg
    16501 agaagaagaa tgaagaaaaa aagattgttt gctctaataa ctagtgcctt ttttatttta
    16561 agcgcttgcg gtacagctgc cgctaatcag gaagataaca gctggagtaa tgtagaaaaa
    16621 aagaaagaat tagtagtagc gacttctggc acactgtttc ccgcatctta ttataatgat
    16681 caaaatgaat tagttggtta tgatgtagat ttggcaaaag aggtggctaa acggttaaat
    16741 gtcaaaatct cctttaaaga atacaacgtg gatggacaaa tcacttctgt ttcaaaaaga
    16801 gaatcagatt ttgctgcgaa cgattttggt atcacaaaag aaagagaaaa aaaattctcc
    16861 ctttcagaga ctttcaaata ttcatttgac agtatgatcg tacgaaaaaa agatgattca
    16921 ggaatccact cacttgaaga tcttaaaggg aaaaaagcag ctggcgaacc caatactagc
    16981 tatatgcgtt tagcggaaaa acacggagct aaattagtca catatgacaa tgccacgaat
    17041 gatcagtatt taagcgatgt agcaaatggt cgaacagatg ttattctaaa tgattattat
    17101 ctgcagaaaa tggccgtatc tgctttacca gatatccctg taaaaatact ggagaacgtt
    17161 tatttcaatg aaaacgaaag tggtttttta ttgaaagaag gaaatgatgc tctgaaaaag
    17221 aaaatcgatg gcgtattaaa agagatgaaa gaagatggga cgacaaagaa aatatctgaa
    17281 aaatattttc aaactgatgt ttcagtcaaa ccgaaagaaa cgatggctga gtcggtgagt
    17341 gagtgatgta ctttcctcta attgattttt ccctcatgtg gacttcactt cctttcgtac
    17401 tgcaaggctt ggtatacaca ttagggattg gcttcgtgtc gtttctttta ggaaatctgg
    17461 tagggttgct tttgacagtt ttaggtcttt tggattggct gcctctgaat gtatttattc
    17521 gtttttattt atcgttcttt agaggcattc ccgcactagt cctgctgttt ctgttatatt
    17581 ttgggttacc ttatcaatta agtgccttaa cggcttcagt catctgcttt acgattacca
    17641 gcagtgcatt tattggtgaa atctatcgag gatcactagc aggcgtttct tcaggtcagt
    17701 gggacgctgg tttagcttct ggtttttctt tctttggggt attgaaatat atcgttttgc
    17761 cgcaagcttt tcgaatctca gtaccggcac tcagtaacgt agctatggat ctagtgaaag
    17821 gtacttcgtt agcagcaaca atcacagtgc cagaaatatt tcaaaaagca aagatcatag
    17881 gcggtcggac attcgattat ttttctctct atattttagt tgcactgatt tattgggtca
    17941 tttgtatcgg gatcgaaaaa gtgcaacagt atttagaaaa acgttctatg catcagtcaa
    18001 tctgaaaaat agaaatggtg ctagcgtggc tggcaccatt tctatttttt gtttcttgta
    18061 tattctgcct agtattttaa aaccatttca tgtaaaatgt aaatcgaaaa tatgttcgga
    18121 ggtagaaaac atgaagaccc cgctaaccat cgaaatagaa tcctcgttat accaatattg
    18181 catcgagcaa ggcggcttgg ttgtagaaga agtcacgatg cctgatgacc aaggaattgt
    18241 agatacgctt agctgcttga caaaatttga tggaagccga gaatggcgat gctatgagct
    18301 gaaagtgaca aaagcagatt ttcgctctac agccaaactg tcatttgttg gacattataa
    18361 ttattttgtt ttaccaagta aactatatga agaagtaaaa actgaaatcc ctacaggaat
    18421 aggtgtttta gtgtatcgtc cgtacacggt tgaagaggag atgcctgcct ctggtacatt
    18481 tctgattgcg aaaaaagcag caaagaaaga gcttgctgta ccggaagaag ctttgacaaa
    18541 ccgcttcatg gcttctttat tcagggaagt aagaaaagca aagcaaatgg agcgaggcgt
    18601 aaaatatttt ccaacagatc aattgtacaa agaattgaaa agacgttcag aaaatcaaga
    18661 tccattttct aacgaacgat attatgaacg gtttttagag gaaatagcta gtcaggcaat
    18721 caatgagttg caagaagaag ttatcgcgct tcagcatgat tatgaatatt tgaggcaaca
    18781 acagcagatc cgtcggttgc ctacagagcc attggagtga aaaatatgta ctatccttat
    18841 ttaagaggaa aacaatttga cctattagca ttaaaagaag cactgtcgcg aggattgctc
    18901 tccaataaaa tccaaccagt gatcgaacca gtacgtgatt ccgctacgct taaaaatgtg
    18961 atcgaattat ttcaaaaaaa gcaccatcga atcgctgtga tccaaaatcc ccaagtagga
    19021 caattcaaat tattcgatca gcatgtgcac agttggacag tcaaagaaaa tagctctgtc
    19081 gtccctgcac agatcttaac gccagaaaat ctcagtgaag tactggattc acctccagcg
    19141 tttcttgttt tcgatggaca gcactaccca aaagatacag aggtgtggaa acagttggca
    19201 ggagttgatt cgaaattttt gataccagat actagtcgct ttcgcatctg gctgcccgaa
    19261 aacaaaattg tgatccgtga ctcttttcaa acgagaaagc atgttgaaag ttatgcagat
    19321 aaaacagatg acttttttag tgatgactat ctttttttcc acgcagacgg atatattgga
    19381 ttttctgatt tcacgatcga aggtagccgt tatttcgata aagggttccc tagccgagcg
    19441 cttgctatcc atctaacgta tattgatgct tacggcaata tacgcgtgaa acattttgtc
    19501 tctgacagta acgactctgc taaagaccaa gcattgaagt ttcttgaagc aggagataaa
    19561 atgaaaaaat ggatcatgcg tcaccatcat caactcttga ttacttcagg aatgattgaa
    19621 ttattggctc tttaccagca acaaaaattc cctggactgg gagtgttgaa aaagtggtca
    19681 cttatgcatc atttggaact tattagtcac ctgctagatc atccaaaaga ttggcttaaa
    19741 tattattcaa aagttcagga actatatgaa gtgatccaat gattagataa tgtaaatgaa
    19801 aaaggaatag aaaagaggaa actggatgta tattcggcaa acaaggaaag aagaagtaga
    19861 agaagtggta gaaatcatta atgccgcagc ggatttcatt gcttcgcaag gctctcctca
    19921 atggcaaaac gggcagaaac caacaaaaga aacgattttg aacgatattg aaagaaaaga
    19981 aagctatgta ttaatagtgg aagatcaaat tgctggaaca gctgcattag tctcaagcgt
    20041 ggatcccgtt tacacaaaaa tagatggtag ttggaaagaa tcaactggtc cttatttatc
    20101 gattcatcgt gtcgctttag cagaaagtac tcgaggaaaa aaactagcaa aaccgttctt
    20161 agaacatttg ttgacgattg cagccagcaa aaatatccaa gatatccgta tcgatactta
    20221 tccagctaat gaaccgatgg aaaagaccat ctattctgct ggattcactt atcaagggat
    20281 gattgaattt ccttttgaac acggcgaaag aaaagcatat caaataatca tcaattgact
    20341 ataaaatcat ctgagcaaag ataaagacat taacactact gaaaaaaaca atcatatgat
    20401 agccaagagg ttgtaacaga agtggccagc ttcaaaaaac aagaaagaat ttccgaaaat
    20461 gcttctcaaa tttttgtgga attttagcgt attttacgaa atagctgctt tctgaaactc
    20521 ccttcatcga gaggtcgtga catagctttt gtcacgacct ctcggcttat cctacgagta
    20581 ttattcgata catttatatc aataatcctt tttttatttt atctgcagct tcttggaacg
    20641 ctccatcgtt tgacacgact aaaagtgagg ggatagcttt caaacgtttc aactgtgcta
    20701 aatcttctac ataaaccgcc agtctggctg aaatctgttc agatgaatct ccacgttttt
    20761 tcaggcgcga ttgcaatgtt tctttagaga tcgtaataaa gataggacgg actaccgtcc
    20821 cgaaatactc atataaatgc caaaaaccat ttgctgtcaa aacggcgtaa caatttccta
    20881 ctgtcagttt ctttttaatt tctgctttac tggaaccata tttatgttga gcatattggt
    20941 cccactctgc aaactcattt cccttgatca tctgattgaa tgtattgtca gaaacaaaat
    21001 aataatcctc ttgatctttt tccccaggtc ttggtgatcg attcgtgtaa gtaatgatct
    21061 tttgttccgg ctgaaaaata gcttctgcga gtgaagtttt tccagaacca gaaggaccga
    21121 taaatacata gcaataattc attgtcattt ctcctttgat gattgaacaa gtgaaaaagc
    21181 tttggaaaat atttccaaag cttttaaaag taactttgct tttttgtata ttataccagc
    21241 aagccagtca caatagcact taaaaagctg actaaagtcg ctgttagcaa aattttcaat
    21301 ccgtgttttg cgacaagatt tcctttttct tcatttaatc ccttcatcgc accagaaatg
    21361 atcccgatag aagagaagtt ggcaaatgaa acgaggaaga cagatataat tgctgttgta
    21421 cgctcagtaa aatggaaatt accatttgct aattctgtca tagcaacaaa ttcatttgta
    21481 accagtttag tcgccatgat actaccggca tccactgctt ctttccatgg gattccgctg
    21541 atgaaggcaa gaggggcaaa gatgtaacct aggatctctt ggaaagtgat gccgaatatc
    21601 ccgtggaaga tagcattgat catggcaatc aaagcaacaa aaccgataag catagcagca
    21661 accgtgatcg ccacatggaa accatcaaga atgtattctc ccaacatttg gaaaaatgtc
    21721 tgttctttgt tttcttcaat gatcagctca tcttcttttt cttctaatac atagggattt
    21781 acaattgaag ccagaatata accgccaaat aagttcaaaa caagggcagt gatcacatat
    21841 ttagaatcga tcagtgccat ataagatccg acgatcgaca tagaaactgt agacatagcc
    21901 gatacactca tggtgaacaa acgtttctca gataggaagg gtaattcctt ttttatggaa
    21961 ataaaaactt ccgattgtcc taaaatagca gaagctactc cattataaga ttccaatttg
    22021 cccatcccgt tgattttact taatcctaga ccaacagctt tcataaataa tggaagaatc
    22081 ttgatatagc gtagaatacc gataatagca gaaataaata caattggcat caatacggaa
    22141 agaaagaaag gtgcacttcc tttatttgct acaccggcaa aaacaaagtc aacaccttgc
    22201 ccagcaaaag caagaagatg atcaaacatt tttgccaata cagaaacaac cgctgtacca
    22261 aaagttgtac gtaataatac gaaaccaaga acaagctgta aaacgaacat cacaattagt
    22321 ggtttcaatt tgattgcttt tttgttactg ctgatcaaaa aagcaagccc taatgtgaca
    22381 aataaaccaa taatacctaa taaataactc aaataatcac tcctattcca aattccgttt
    22441 tattttacct gttttgcaaa taaaatacta atctttagtg aaattttcat tactttttta
    22501 gagtgaaacg cattcatttt ccattagaaa ataaagcgaa caaaataaaa ggtttaaaaa
    22561 gtgtaacgtc ttagtaaagt ctataaaaaa acgaaactat cgattaagct aatagtttcg
    22621 ttttcaacat ttataagtaa actttttcta ttggtaatat agattttcgg aagggtagac
    22681 aggtgcgcag gtaatatcga ttcctagttt tttcaatgtt tgttcatcat ttttattgac
    22741 catcacagta gagtgggctt gtgctccctc aagttcagct agtttatcat aagcaacttg
    22801 agcgattgga ttcgttactg cactgattga taaagcgatc aagacttcat tgatgcttag
    22861 tgcatccaaa gaggaatgaa gtgtctttga tttcaatcct tgtattgttt caagaactaa
    22921 tggtgacaat aaaggaatgt catctgcgat atgggctaaa tgttttactg catttaagat
    22981 aactgccgct gacgcatcca tcaatgaact tgtacgcccg gtaatgatga cttcatctgg
    23041 taattcgatt gcaatcacag cagaaggatc ttttgtagga ctttgcgctt gaatcttatc
    23101 tgcatagtca tgggcaggga ctaccacgtt tcggtcttca ggacgtaaac cgacttcttc
    23161 cataatcagt ttgattcgat taactgcttc ttcgtcgatc aatccttttt tgaagtcgca
    23221 ttctgtttgg aaataacgac ggatgatttc ttgtttagag gcttcttgga caacggtatc
    23281 atccacgatc ccaaatccta cacgattcac acccatgtca gtaggagatt gataaacaga
    23341 ttctttacca gtaatttttt caataatacg tttgattaca ggaaatgtct caacatcccg
    23401 attatagttc acagctactt tattgtaggc atcaaagtgg aaggaatcga tcatattgac
    23461 atcttttaga tcgactgtgg ctgcttcata agcaatgttc agcggatgtt tcaacggaac
    23521 attccatact gggaaagttt cgaacttgga gtaaccagct gcatgtcctt tttggctttc
    23581 atgatacagt tggttcaagg aagtagcgag ctttccactt cctgggccag gagctgtcac
    23641 tacaacgatt ggtttagttg tttcaatata ctcatttttt ccaaaaccat cttctgagac
    23701 gattttttct acgttgactg gatagtcttc gatttctgag tgtttataga ccttgatgtt
    23761 tcgtctttct agtttattga taaagacttt cgtggcaggc tgtccactat agcgagtgat
    23821 aacgacactg ttgattaata agccataacc acgtaattca tcgattaacc gcaagatatc
    23881 catatcataa gtaatcccat aatcaccacg gatcttatta cgttcaatat ctccagcata
    23941 gacgcaaatc agtatttcag cttgatcttt caatttttgc agcaatttga ttttcgcatc
    24001 ttcttcaaat cctggaagta cacgtttcgc atgcttgtcg tcaactagct tcccaccaaa
    24061 ttctaaatat aatttgtcat agtggttcac tcgttccaaa atatacgctg actgctcttc
    24121 aatatatttt tctgaatcaa aaccaatttt tttcactagg ttccctcctt aagtactaaa
    24181 gcattttttc atttttttga ggattcgtca actggaagag cgaaattcct caaaaaaaca
    24241 aagaaaatgg tttaaaccaa cttgtctaga tagttattca ttggtatgat agtttttgat
    24301 aaggaaggtg ctacatgaaa aaaagacaga aaaagaaaaa cgcatataaa cactatatcc
    24361 gttcgatttt taccggttat gaaagaatgc ttgaagatcc cgaattggaa caattgacgt
    24421 ttacttatct aaacgaagaa acgcagctaa ctcgtgatga gcatcagcgc attcatttca
    24481 caacaagaga tctgcctagt aaataaaaaa taagataaag gtcatcggat tcgattctgt
    24541 cctatcagat ggaaagacta gttgatcgaa aaaaaggctg taccaaattt tggtgcagcc
    24601 tgttttatgg agcattgccc gctctttttt ataacaattt tttacagaaa atggtactat
    24661 tgaagtatta ataagtgaaa aacagatgag taagaggagg caagtagtat gaaagagcca
    24721 gatacaaatg caaattatga gccagacgaa ctagaggaga cgaatgcgga tcaggataca
    24781 ggacaagaat cgtcttcttt ttacgatatc aaagaaaaag caagcatgaa tctttcccaa
    24841 ttattagaag acttacaggc ttttgaaaaa gctgtccgcg aagaagatat ccctgagatc
    24901 taccgtttat acaatggccg attgaatgaa gaattaaaaa gaagctcaaa tgaaaatcac
    24961 gagatcgatc aattgctgat gcgtaagatc catgatcgtt ttttacagga atttcctttt
    25021 atggaacaag tagaaaacat ctcgccaacg atgagctatt acaaaatcgg cacttattac
    25081 catgatcggc ccacggtcgg tattgatgca agtctgccag aaatttttgt tttgcctaag
    25141 atcgatgaag agtgggagaa atattcccaa ccggagtcaa cggactatga caaaaaaatc
    25201 aatgagctag acgcaaaggt gatcactgct cagacagaaa ttgaacgttt agatgaacaa
    25261 ataaaagaaa tcaatcgaca aatgactgat ttgaacgaca ataaaggatt cttgaatcgt
    25321 aaaaaaatcg aggaagaaat tcaagaatta gagaagaaga agcaagtact atctaatgaa
    25381 aaattaggct ggcttcctta tattgagacg cctgaaacaa tccagcagca aaaagaagca
    25441 ttgaagcaag aagcaagagc agaccagctt cgtgcagcca tcgtagaaaa agaacaaagg
    25501 caaatcagac gctatttcgg tagcaaagag ggattcggtc aagcaatcca tgaattcttg
    25561 atgaattatc ttggcaatga gaatcctaat aatcaatcta atgaaggagg ttccgagtat
    25621 gaatgaacac gatgaagcag aatggacaaa aaatgaagaa caaacagaag atcatgaaga
    25681 aacgaacact tcattggaag aacaaacaag taatcctctg gatgacacag aagaaatccc
    25741 tatgctagag gaaacaaaaa tacacgaaaa agaaaaagta actccgacga atacgaatac
    25801 tgcagaaaac acccatgctt acgaaataac tgaaaatccc gggtatttag aaggaaaaga
    25861 gcaagatcat caagatatcg tcagtttgat tgaaacagcg gaagcagaat tagcggattt
    25921 acgtatgaga aaagatttga tcgaatcaga ttttccacaa ttattagaat catatgatcg
    25981 ttttatattg aatgaagaac caatctccac gatcggaaag acaagcctga tcgaatactt
    26041 cacgtatgaa ttactgaaac cgttgaatga tattggtctg gaacggtcaa attcagctta
    26101 tgacacatgg aaaacaagac atttttcgat caattatacg ttgaatggac aaaacgaatt
    26161 agtcttttcc tttgtattac cgaccatcga tcaacgatac caagtggaat cgatgagtct
    26221 cctaaacgta tcaccagaga caatggaaat caatgtccaa gatgaccggg tgctttcttt
    26281 gatccgacac tggtcagtgg atcgagtatt ctctgcaggg cagatcacga tattcaatca
    26341 caaattgaat caattattag cccatgcaag aacacttggt tttaccgtta atcagacatt
    26401 actagacaat acgaagccac ttcgtttgaa tcttcagagc gaatttgaat tgacgaatga
    26461 agtattggat gatattttta tcgtggcaat gaaaaatcca tcctacgata tggaaaagat
    26521 caacgaaact acttatcaag tacgattaga taaaggacag tctttaacga taacaacaaa
    26581 cgaaaaagat caaacagtct tgtcgatctc atctggagac tacccacgat ctgtcattga
    26641 tttctttatc aattacgaat tcttggtccc attaatggtg agaaagtcct gattggataa
    26701 gttggattac cagatgtcat tcagataatt ctgttccaaa ggacttgcgg cagttatcca
    26761 gaataattgt cacaagtctt ttctgtatgt actaaatgga cgtgaataac ttgctgatga
    26821 aggagggatt tcattaggaa tgtccacaaa taaaatagca cagcttagct tattgtctgc
    26881 tgcttgtgta gcaggtagga tcgcttttca atttattccg aatttccagc caatgactgc
    26941 tattttttta tttatagtac tttacttaag cttaaaagat gcgttaattg tcatgagtct
    27001 ttctattgcg atcagcagtt tttattttgg tgttggacca tgggtcatcg gccaatggat
    27061 cagctatact gttgttctga gtttgttttc aatcgtctgc ttgagaaaaa cagtgcaaag
    27121 gaatctatgg ttaaagggag tctgtttttt tctggcgggc atggtttacg gaataggtat
    27181 gaccgttttt gatacgctgt tatatcgttt gcctcaacct tggatctatt accttcaagg
    27241 ggtgtcgttt gatttgatgc acagtatagg caatgttgtg ttctttcttg tttttctgcc
    27301 agtcgtcaaa cgtttttaca atcattcagg aggaaaaaat gaaaaaaata atttatagtt
    27361 tgcttgcaat gagtgtcagt atactcattt tgacaagttg cagtacaacc tatacacaat
    27421 cggcttccaa ccaaaaggag gaagtaaaaa catcgcaaaa agcaacaatc acgttacaag
    27481 aaaatggtca aaacttttcg gaaaaagaag tgttcttcaa aaaaggcgat gatttactga
    27541 gtattttgaa acgaaatttt gaagtcaaag aagataacgg cttcatcact tcgcttgaag
    27601 ggcactcaca ggatgagaaa aatcaactct attggatgtt tactgtggac gggaaaagtg
    27661 ctactactgg tgccaaagag atcaaattga atgatggaca atcggtcatt ttcaatttgt
    27721 ctaaactata agtttcataa aaaactgtta aagggcgaat attgccataa ttcagtgagt
    27781 tcagttgatg aaagaaggaa aataacatgg aaaattttaa aaaaagcgga atgttttccg
    27841 taattgctgc tctaggggct aatattctag ttgctatcag caaatttgtc ggctatgcaa
    27901 tcagtggcag tgccgccatg ctaaacgaaa gcatccacag tatcgttgat tgcagcaatc
    27961 agattttgct gttattcggt gataaacaag ccaataaagg acagagcgag ttgcatcaat
    28021 ttggtgaagg acgagcaaaa tactttttca gtacaatcgt cgcaatgatg cttttcttcg
    28081 gcggtggggc acttggggtg atggaagctt ttgaaaagtt gacgcatcca tcccatgaag
    28141 tgggtaatcc gatcattgtt atagcaatac tgttatttgg tttggtaatc gaaggaagtt
    28201 cgttacgcgt ggcaatgaaa gaaatcaaag aattgaatac tgaaaaactt tccacgatgc
    28261 gctttttgcg cgaaagtcga cacagtgaaa tactgattat ttttacggaa gacttgtgtg
    28321 ctgtaatcgg tttggtcttg gctttagcag gaacaatatt gacttatgca accgggattg
    28381 ctgcatttga tgcgatcagc ggattgctga tcggtttctt gctgatggga gccgccattt
    28441 tcctagcaaa agaattttat agtctgatca tcggtgaaag cgtgacagca agcgatttat
    28501 caaaaatcaa gttagctttt gaacgtcctg agatcagtcg gctgatcgat ataaaaacag
    28561 ttcatttaag tccaacggag attttagttg cagcaaaagc agatatcgtt ggtaacaaag
    28621 agaatgaagc ttactcggtc atcaatgata ttgaaaaaaa tatgcgtcag tctcttcctg
    28681 acaagaagat gtatatatac attgaaacag ataaatatga cccaaattat tctcgtaaat
    28741 aaaacgttta ttcatgaatt gaagcaaaaa tgagaaaaat ataatcttgt ttttaaataa
    28801 aacggctaca atttagcgaa tttatgttac aatgagtccg ctgtttattt gttgtaatta
    28861 aaggagatat tttaatgaac aaaaagaaaa gtgcgccatg gcttcaagct ttgcatgcag
    28921 tgatgccact ttgtataagc tatataccag taggattagc ttgcggagta ttgttgcaaa
    28981 aagttggatt tgatcctctc ttatcaggac tgttatcgat tctggtcttt tccggtggcg
    29041 cgcagttttt agttgcatct atgcttacga cgcaagcatc atttgcaacg acattattga
    29101 tggttttctt tttagaatta cgctatacat tattaggagc aagtcttgcc ggttttatga
    29161 aaaaagaaaa gcggacgttt ttggctgtct tttctcaatc actgaatgat gaaaactatg
    29221 cggtgaacta cttaaagttt tcaactgacc cttcctggaa taaaagaaag gctttatatg
    29281 tcaattggtt ctcaatgact gcatgggccg gtagcaatat gatcggtaat tttttcggct
    29341 cggtgattcg agtagatgct gaccttgtcc attttgcttt gacggccatg ttcattttca
    29401 tgtttatcat gcagatgaaa agcgcactac tgatctttac aggtttattc tcaggcgtat
    29461 taggggtcgt attcatggta ttattccaaa atactttcgg attgattgca gcaacagtca
    29521 ttgcttcatt tgccggattt atgctggaga aggcattcaa aaaagaaaaa gctaagaaac
    29581 taagagaccg cggtaaagat gtcaatgagc cgctcttcaa cttcccggaa caggaggcca
    29641 atcaccatga ctaaactata tttgctactt ttagttgctt gtctgttcat cgtggcatat
    29701 atcccgcgct tgttcccgat gctttatttt actcatcgaa aagtgccatc ctggttcagt
    29761 gactggatga aatatgtacc ggtggcatta tttgcggcgt tagcttttaa agatgttttc
    29821 attactcatg aacatctgga tattgcttgg aatatcaaaa tagctgcaat gattctagta
    29881 gcaggcgtgg cttacaaaac acgttcgatg gcgttgtcag ttttgacagg acttgcgtct
    29941 gtctttttgc tgtctatgct ttaaactgtt ttattttatt aaaaacagag attgcgacat
    30001 aagttgttca aactgatcta tccgaactgc atttgcaaca gtcaattctg gatgcaagtt
    30061 tattcgtagg gataaaaaag attagtgttg gcttatgatg caagctcttt tttattttag
    30121 gaattagaga gggatttgtc cttctatgat tttttgctat aatagggaag atgtttattt
    30181 tatgaaagaa tagttgaggg aatttaaatg aataaaaaat tgatgcaagg aacattttgg
    30241 ctaacctttg ctaatcttct atgtaaagtt ttaggggtag tctatttgat tccctggcta
    30301 agtatgatgg ggaacaatca agatggtatg cttgctacga cattgtataa tgttggctat
    30361 cttccttatg gattgttcct gatgcttggt acagtcggat ttccaaatgc gattgcaaaa
    30421 aaagtagctg tagccacaaa agaaggagat aacgctgctt gtcggctgat ttttagaagt
    30481 acgatcaata tcatgtttgt catcggtatc gcctctgctg cactgatgta tttatttgct
    30541 cctttactag ccggaatcag tccgattgct aacgttaata atgggatact agctatcaga
    30601 agtttatgcc catctttgat tgctatccca attcttagtg caatgagagg atatttccaa
    30661 ggaaaaaatg atctgcggcc ttatggaact tctttgatta tcgaacaggc tgtgcgagtg
    30721 atcgttatct tggctggtac ttattacttg cgcgtattga ctgacggaac catattagaa
    30781 gctgtattga tcagtacagt cgcttctttc ttcggtgggt tagctgcgat tatccatatg
    30841 tatatcgtcg gacgcagaaa agacttcttc gaacttaaag attttttgat ttccagccgt
    30901 tatttcgaaa gagaaaaccg atctgcctct gtctctatca ttcgggaaac actaccgttt
    30961 atttttgtcg gctcagtcat tactatcgtt caaatgatcg accaagtaac gatgaaacca
    31021 ctgcttcact ttttccgtcc agagatcgca gatcagcaac tggaattctt attcagccgt
    31081 gcttctgtca atccaaataa attgacattg atcctgatct ccatggtagg aacagtagct
    31141 ataagcagct tgccaatatt aagcacgatg aagaaaaaag atcggataca aatcgaaaaa
    31201 acagtaggag atagtttttc gattgcgtta ttgattcttc ttccttcatt gactggaatg
    31261 tctttattag ctggaccact atacacattg ttttttggct atgatcctga aagtgtcgga
    31321 tatttccaaa tggctttgct agcttctttg tttttctctt tattcacgat cctatctacg
    31381 atgcttcaat ctttgaatca ccatcgtttt gcgatccgat taactgtaga agcaattatc
    31441 ctgaaagtca tcttccaagt aatcggatta ggattagttg gcggatacgg gatgagctta
    31501 tcaaatggct tagcattcgg tattgtcttt gttcgcggct atcactttat gtgcaaagaa
    31561 taccgaatag cacctttagc aaagatcagc aattttttct taaaaacttt ccgctccacc
    31621 ctgattatgc ttgcagtatg ttttgccgtt ttctttttgc tgaatcagcg attgtcaatg
    31681 gaatcaaaaa gctatgcaat gatttattgt gtggctgtag gaagtattgg cggcttagta
    31741 tttttatttt ctcaattcgg aaaaaatggg tttcgaatgt tgaaaaatgt taaacatcat
    31801 agcagaagca aagaataggt taactaatcg ttttttgctg tcacttgaaa aacggcaact
    31861 cgtatgatta cgcttgtcgt ttttctattt tccaatttta gtttcatatc taatcttact
    31921 tatgatatag ttataaggtc tggaaaaatt ctttgaaacg aggaaattaa acacatgaaa
    31981 cttgcagtag ttacagatag ttcggcatat ctgccggaac gtatcaagaa ccatcccgac
    32041 ttattcgtta ttccaatccc tttgattatg gatgggaaaa tttataacga aggaatcgat
    32101 atcgaagcgg atgaatatta tggactttta aaaaacagta aagattttcc tactacttca
    32161 caacctgtcg taggggaagt tttgggttta tatgaggaat tgaaagagaa agggtatgac
    32221 acgattttaa gtatccatct ttcttcaggg atttctggct ttgtcaacac attatttgcg
    32281 atgaaagatg acatcagcgg agtaacggtc attccttatg actctaagat cactagtatg
    32341 ccgatgggac acatggtaga agctgcctta gacttaaatg atgaaggtca gtcccttgat
    32401 gaaatacttg cacatatcga tcgaatccga gataatacct acgcttatct gattgtagat
    32461 gatttgaaca atttagtacg aggaggacgt ttgacaaatg gtgcagcgct gattggcagc
    32521 ttattaaaaa tcaaaccgat cctgacattt tctgaaggga aaattgtttt attcgaaaaa
    32581 atccgttcaa gtaaaaaagc atttgctcga gctgaagaaa ttattggtag aagaaataac
    32641 gaaatacaac cgccagtcaa actttacgtg atacatgcaa ataatttaga agtagcgatt
    32701 gcggagaaag agaaattgca gaaaaaatat ccaaatgcag aaatcgaaat tggtcatttc
    32761 ggaccagtca ttggtacaca tttaggtgaa aaagccatcg gcattgccat ttcagcgcaa
    32821 taattccttt tattcaaaaa acataccaaa aatattccaa catcatcttg gactttctac
    32881 atgttactaa tgagcgtgta aaagaacgag atggtgtttt tatttctatc aatgaagctc
    32941 tttttctttg caatcctcac ttcaatcctc tataatgtcg catatagttt agtaaatgag
    33001 taaactacta atttacttat ttactaaaaa taaaaggatg aggagttaaa ataatggaaa
    33061 taccaacaaa tttaaaacat aaaccagtaa tcgttgtaga agactatgat aaagttgatg
    33121 gaagaaatgc attaaatacg gatgcaaaag ggctttcact gggccttgct cagtggaatg
    33181 atcgtggaaa agtcgatatc tcaggcaagg tatggcgaca tactggagaa aaatggtcac
    33241 gacagtcgga agaattacca atcacacgta ttttagattt agcgatctta gctgcacaag
    33301 gaagtttata cttccaagat gcttatcgtt atgaaaaatt ttatgatcca gaaaatccag
    33361 tcgtagacat cattggatta caagggaacc gaatgacggt agaagtagat accaaaaatc
    33421 caaagatcga tgaagacatc attttgtatt acgatacact acaaaaagac ggagaactga
    33481 ttggggaacg attccgtatt ttaaaacgtc ttttagatga gctaggatat taatagacag
    33541 agatgatcaa aaggggaaga gaagcatgga gaatttctca ctaacagaaa accgaacctt
    33601 ttcacggcta gaaaagcaaa tgatctggaa gaaaccgaaa agccatcgga caagtgcaga
    33661 agaatggcgg atcgcctccg aaatcaaagg gaattggcaa gatcctgaaa tgaaaatcag
    33721 taacgtactt ttggaaggag atgccgggtc ggggaagaca caattagcca aagcactatc
    33781 tgctgatctg caacttccct atacaaaagt gacctgtttt gcagatatgg acaaatcaga
    33841 tgttttcgga gccttattac ctgtagtaga ttctaataag gaacaagacc aagaattact
    33901 agaagctatt taccaaacag atacacttga agctgtactg acactgatcc agaaccatta
    33961 cggcattgat cctttttctg ctaaagaaaa attagcagag ctagttcaaa ggatcgaagc
    34021 tgattatcca gatgcaatcc attacagcta ttacccttct gaaatcgttc gagcgattga
    34081 aaaaggatat ttattagaga ttcaagagcc gactgtcatc cgagatgcct ctgttcttgt
    34141 ggcacttaac tcagcgctcg aaccaaatgg gatgttgaat ttaccgacag gaatcattcg
    34201 tcgccatcca gattgcgtca ttgtgatcac tacaaatcga aattaccaag gaaatcgccc
    34261 attaaatgaa tctttaagag atcggatgca acatgcggaa aaaatggatc ttcctgcctt
    34321 agaagtcatg gcagaacgtg cgatggcgaa aactatgatc caaaaacaag aacttctctt
    34381 aaagatggca gagattatcc gtttgttgga tgaaactgca aaagctaacg ccatcaaagg
    34441 tgtagcagga atgcgttctt atttttattg ggcaaatacc tataagcaag gacaagattt
    34501 actccagtcg atttatccaa aagttttgta taaattatcc actgattcag atgaattggc
    34561 aattcttgaa caagctttgt ctgacagtgg attgctactg gaacttcagg acatattgag
    34621 aaaagaaaaa tggggtcatc ctcaatctga gcatacaaaa ggaagaacca ttagcgccga
    34681 agaagcaaat gaacgaaaca tcactgaaaa tgctgaagaa attctgcgct tagatgaaaa
    34741 gaaatcgttg tctgctgaag aaccaaatga gggagaagag aaacaagaag aaacaataga
    34801 aaaaaacgag attcctcctc aaatgcaaaa agaacgcagt agcgatatcg aaggaaaaaa
    34861 tcaaaaagaa caacaaacag gagatgattc tcaagaaatg tctgcttctg acatggaagc
    34921 acatgataag gaaatcaaga agcaactgaa taaagaagcg cgacaaatca tgaaggaaac
    34981 gattcatgaa aaagaaggtc tcatcgttca ccgaccagct ttccaaggaa aaacgccgga
    35041 agcacgtgag ctacacatgc aagtaatgcc aatagtagaa tcacttagcc ggcaaatttt
    35101 agagttactg gataatgaac aaacagagac gtaccaaaaa ggaaaatacg aaggtcagcg
    35161 gttcaatgca agcaaagtag catatggtga tttgagaaat tttgacaaaa agaacccgcc
    35221 acatgagcaa ccttctttag cggtagctgt acggatcgat gaatctgggt caatgatacg
    35281 ggatgatcgg atcgaagcgg caaaaaaagc agcgattgcc attgccgaat tttctaagaa
    35341 agtcgatatc ccattgttga tttacggaga tacagcagac cgttcgtcaa gagaaaagac
    35401 ttctttgttt tcttataaag aatttgaaga cggatttcaa tggttaaatg aaaaattagt
    35461 cactatgaaa ccaaggcaaa acaatcgtga tggtgctgca cttcgtttag cagcagaaaa
    35521 attaaacaga caatccgcaa caaccaaact gttgcttaat attagtgacg gtcagccaaa
    35581 agcgttgccg gattatacag gcaaaaaagc aaaagaagat atccagtctg ttttgaaaga
    35641 atatgaacgt caaggaatcc tatttgtctc cgcagctatt ggccaagaca aagaagaaat
    35701 caagtcgatc tacggtgaaa gtcgttttgt agatattact gacttgaatg aatttcccaa
    35761 gcaaatgatt caactgattg cccgctatct atgataaaat aaagctcaac gaacgcactt
    35821 agcagattcg ttgagctttt cgtttctagt taccggtaac aaggatactt ctaaaataga
    35881 atcggttata atcaagaggg accgttaaag gagtgaaacg atatgataca agaacaaaaa
    35941 tggtggaaag aagcagtagg ctatcaaatc tatcctgcta gtttcaaaga tagcaatggc
    36001 gatggtcaag gagacatcaa tgggatccgt gaaaaactta attatttgaa agatcttggt
    36061 atcggcttta tctggatcaa tccgatctat caatctccat ttgtcgataa tggttatgac
    36121 gtaagtgact atcaagatat agaagagaga ttcggaacga tggaagattt tgatttattg
    36181 ctaaaagaag cacatgaatt agggatcaaa gtgattatgg atcttgtcat caaccatagt
    36241 tcggatcagc atcaatggtt tgaagaatcg aaaaaatcta aagaaagccc gtatcgcgat
    36301 tattacattt gggtcgacgg ggtagatggc aaagaaccga ataattggac atcgattttc
    36361 ggcggttctg cttgggagta ttctcatgaa actggacagt attatctgca tgtttttgca
    36421 aaagaacaac cagacttaaa ttgggaaagc gagaaattga aagaagaact attcaatatg
    36481 atccgatggt ggttggataa agggatagat ggatttcgtt tagatgcgat ttctcatgta
    36541 aaaaaagatg aatattcagt aaaagccaca gagaaccctt tctcaccctt tcaaaatgtc
    36601 tcaggaatcg aagaacattt gaccgatttg aaacatgttt ttgaagagta cgatatcatg
    36661 actgtcggag aagcaagtgg tgtcactgca gaagaaggac cacaatgggt agggaaagac
    36721 ggttatttcg atatgatatt cgaatttgat catatccata tctggcagca agaaaaagaa
    36781 gggcaattag atgtccttaa attgaaactt gcattaagcg cttggcaaac ttcattggat
    36841 ggtatcggat ggaacgcttt atatatggaa aaccatgatg ttcctcgtgc cgtttcagtg
    36901 tttggagata ctcgtcccga tttttgggct atgtctgcaa aagcaatcgc tatgatgtat
    36961 ttctttttgc aaggtacgcc atttatttat caaggacagg aaatcggaat gacgaatatg
    37021 ccctttgaat cgatcgatca agttgatgca gtagattcga aaagacttta taaaaggctg
    37081 cttgcggaag gtaagacaag agaagaagca ttggatatca ttcgtgaaac aacgagagac
    37141 aacagcagga cacccatgca atggacttcg gaacaatatg ctggtttttc tacacatgaa
    37201 ccttggctga tcactaatcc aaataccaaa acgatcaatg tagaacaaca agaatatgaa
    37261 ccagagtcgg tcctgcaatt ctataagaac atgatccgca ttagacaaac caataaaggt
    37321 ctgatatacg gttcttataa agaatatctt catgaacatc cacaacttta tgtctatgaa
    37381 agatatttag aagatgaaca atatcttatt atggtaaact taacagaatc acttgctgac
    37441 tatgaacttc caaaagaagc agatcagtcc tggacattac tcttgtcaaa ttcctctagc
    37501 ggagaatttg aagctaaagg catactagca ccttatgaag ctcggttata taaaacaaac
    37561 aagtaaaact tcctcgttaa agttcattag ttggactttg cgaggaagtt tttcagattg
    37621 ctctgtcaat tactcatttc aataattcgc tctaggacca ccgatatatt taggacgaga
    37681 gcgaagggct gtcacgtgag cttgtccaag ggttttcaaa cgaggtctga caggtacttg
    37741 gacatgtttg atatgcatac cgattgatgt gtcaccgata tcgataccgg cttgtccaac
    37801 tacgtgttcc acttcgacag gatcattgaa ctggtcaaaa gcagcaacag aacaagcacc
    37861 tccagcatgt agtgcaggaa caacagagac aatttcgaaa ttttttgctt ctgccactgt
    37921 tcgttctact actaatgctc ggttcaaatg ttcacaacct tggacagcta gcgcaatttc
    37981 tttaggatcc aacaactctt ttaacgtacg gatgatccat tgaccaactt ccgcactgga
    38041 attttttcca atatgtcctc caacgacttc gcttgtgctg catcctaaaa caaaaagatc
    38101 accttttttc aagtgtgcct cagccaatat gtcattaact atttcagtca attggtcttt
    38161 taattctttt tctgacaact tcattttcta aaaccttctt tttcaattaa tattgcttcc
    38221 gtgcttgaat acgttgaaga cttgcagtta taggaaaggc aagtcccatt ccaatcagat
    38281 tttggaagat gtttcctggt atagaagcca ctccagccgg ccaatcatat aaaatccaac
    38341 cggcgatacc atagccgatg atcataacta agcttcctaa taaaagccct aatccccaca
    38401 tacgagtaga agatttgctg aaagaaccag caacatatcc ttgtaacccg tggatcacta
    38461 atgaaaaaac tgcccactgc ggatatcctg aaatcaaatc gatcaataga ccactggaag
    38521 caccaacaac taatccaccc gttggaccat aaagaaaagc agcggtatag atcccggcct
    38581 cgcataatgt cacaaatcca ttcgtagccg gtacagggat caaaattgtt aatgaaaggg
    38641 caactgtcaa cgcaatcagc atagcataga tcgttaattg cttagttttc atttctcatt
    38701 cctccttttt gaataccgcc agaccagaca cttccggtat gatcgttcat cagcacgcca
    38761 ttagaaatac aatgggcaac aaatttttta ctttctgcta ctgctttggt aaaatctgcc
    38821 cctaacacaa gattcgctgt tattgcagaa gaaaaggcac aaccggcccc gtttactgtt
    38881 gtagtgtcga tttttttatg agaaaatacc tcataatgat caattgaata gagaagatcc
    38941 acagcttcat ctccagccat ccttgtgccg cctttgatca caatgttttt tgttcctgaa
    39001 gcagtgagtt ctttagcgag atggatcatt tctgttaacg aagagggaag agcttgacca
    39061 gaaagtaaag cagcttctgc caaattaggc gtaatcatcg taacaagtgg aaaaagttct
    39121 tcgatgagct gcgaaatata caattgattg atcacagctt cagtttcttt gaatgccaaa
    39181 acagggtcta aaatgattgg gatattttgt tttgttttta tgaagtctcg aacaaccatg
    39241 attgcttcac ttgaattcaa taacccaatt ttgataccat ctaatgaagt cattttatca
    39301 atcgaaacta actgtcgttt aagcaattcc aaagatatag attccagcat aaatcgctca
    39361 ttaatatcta gtgcccctat agaagtcaag gcgcttagtc cgaatagacc aaaattctca
    39421 aaagttttta aatctgtttg gattcctcca ccagcaaatg gatcagaacc gccaattgtc
    39481 aagattcttt tcataacatt ttcctccatg aataagagta taacgaaatt ttcaaaaaag
    39541 aaaaacagcc aattggcata ttttcatctc atccaattta aaaaaacaaa aagaaaagaa
    39601 agaccaaaat ggatctttct ttccttaaat agttaggact tttgtgattt taattctttc
    39661 tcgatttgaa gccgttgcac tttcattgta gctgttcgcg ggattgcatc aaaatcacgg
    39721 atgataggga cgttcaagcg aggaagttca tggacttgct cccaccatcg attccaatcc
    39781 atttctttat caggagctaa agcaataatc ggttgtggag aatgattttt atcccttaca
    39841 atcactactt ctgccaaaaa atctaacgaa tctaataaaa agtcttcaat agctaatgta
    39901 ctattgatcg tttcgatcaa atccacttgc cgatctttca aaaataggtg gccatgctta
    39961 tccataaaac cgtaatctcc gctatcccac caatcgccat aaacattttc ctggaaccgt
    40021 gcatcttcct tgtaataagt caaagcccgt cctttagaca gtaattgtat atgaccatct
    40081 gtgttttcag gtaggacatt cccaactgaa tctgtaatcc gagcgcttgt catgtcttcc
    40141 agtccaacgc ccatatctct tgcatcactt gttttcaaac tctctagtgt atgagcctta
    40201 agaatcattg gtccgcattc actttggcca tatacttgaa gaaataccgc atcctgtgcc
    40261 atcgacgttc gcaaaaatgt cttcattgtc tggttgttta tagcatcaaa agtggagtga
    40321 tagtatcgga tacccgaaaa agcttctgga tgctccttag cagtaaaacg ccactgaaca
    40381 aagttattcg gatgggtctc tagtgcaatt ggccggtgag cttccaacat atgaacaact
    40441 tttgaacttt gtgcgtttgc taaaggcatc ataggaaatc ccatggacat caaagaagag
    40501 ataccaatat tgaatctgga atgcactgga gagatgtgaa aagcgattaa tttcttttcc
    40561 gaaatttttg tgaaaactgt tttttgccat ttcgtccgcc atcccataga atgagcagaa
    40621 tgacaaataa gtttagggat accagttgta ccagaagtat gggtcatata agaaatttca
    40681 tttttgtcta aaaatgattg agaaacagac aaagcaggtt gttccattaa ataaagaata
    40741 gg
//
LOCUS       DAFGZE010000022        37938 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.022, whole genome shotgun sequence.
ACCESSION   DAFGZE010000022 DAFGZE010000000
VERSION     DAFGZE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.022"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            57..3335
                     /locus_tag="LTU54_001179"
     CDS             57..3335
                     /locus_tag="LTU54_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HBL8295902.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3876..5783)
                     /locus_tag="LTU54_001180"
     CDS             complement(3876..5783)
                     /locus_tag="LTU54_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HBL8295903.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5913..6788
                     /locus_tag="LTU54_001181"
     CDS             5913..6788
                     /locus_tag="LTU54_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HBL8295904.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6868..7842
                     /locus_tag="LTU54_001182"
     CDS             6868..7842
                     /locus_tag="LTU54_001182"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL8295905.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8022..9167
                     /locus_tag="LTU54_001183"
     CDS             8022..9167
                     /locus_tag="LTU54_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL8295906.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9304..9648
                     /locus_tag="LTU54_001184"
     CDS             9304..9648
                     /locus_tag="LTU54_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HBL8295907.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9919..11205
                     /gene="efmA"
                     /locus_tag="LTU54_001185"
     CDS             9919..11205
                     /gene="efmA"
                     /locus_tag="LTU54_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HBL8295908.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11343..11942)
                     /locus_tag="LTU54_001186"
     CDS             complement(11343..11942)
                     /locus_tag="LTU54_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HBL8295909.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12346..13467
                     /gene="mnmA"
                     /locus_tag="LTU54_001187"
     CDS             12346..13467
                     /gene="mnmA"
                     /locus_tag="LTU54_001187"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBL8295910.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13713..15134
                     /gene="arcD"
                     /locus_tag="LTU54_001188"
     CDS             13713..15134
                     /gene="arcD"
                     /locus_tag="LTU54_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HBL8295911.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15148..16266
                     /locus_tag="LTU54_001189"
     CDS             15148..16266
                     /locus_tag="LTU54_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBL8295912.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16326..17618)
                     /locus_tag="LTU54_001190"
     CDS             complement(16326..17618)
                     /locus_tag="LTU54_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL8295913.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17750..18328
                     /locus_tag="LTU54_001191"
     CDS             17750..18328
                     /locus_tag="LTU54_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8295914.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18457..18834
                     /locus_tag="LTU54_001192"
     CDS             18457..18834
                     /locus_tag="LTU54_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HBL8295915.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18767..20839
                     /locus_tag="LTU54_001193"
     CDS             18767..20839
                     /locus_tag="LTU54_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBL8295916.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21050..21997)
                     /locus_tag="LTU54_001194"
     CDS             complement(21050..21997)
                     /locus_tag="LTU54_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL8295917.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22243..22707
                     /locus_tag="LTU54_001195"
     CDS             22243..22707
                     /locus_tag="LTU54_001195"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBL8295918.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22769..23812)
                     /locus_tag="LTU54_001196"
     CDS             complement(22769..23812)
                     /locus_tag="LTU54_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBL8295919.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24189..25154)
                     /locus_tag="LTU54_001197"
     CDS             complement(24189..25154)
                     /locus_tag="LTU54_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBL8295920.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25388..26233
                     /locus_tag="LTU54_001198"
     CDS             25388..26233
                     /locus_tag="LTU54_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8295921.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26424..27611
                     /locus_tag="LTU54_001199"
     CDS             26424..27611
                     /locus_tag="LTU54_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL8295922.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27617..27973
                     /locus_tag="LTU54_001200"
     CDS             27617..27973
                     /locus_tag="LTU54_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBL8295923.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            27975..28313
                     /locus_tag="LTU54_001201"
     CDS             27975..28313
                     /locus_tag="LTU54_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HBL8295924.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28389..28645
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28708..29742
                     /locus_tag="LTU54_001202"
     CDS             28708..29742
                     /locus_tag="LTU54_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8295925.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29735..30421
                     /locus_tag="LTU54_001203"
     CDS             29735..30421
                     /locus_tag="LTU54_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8295926.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30445..31293
                     /locus_tag="LTU54_001204"
     CDS             30445..31293
                     /locus_tag="LTU54_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBL8295927.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31490..32263
                     /gene="sufC"
                     /locus_tag="LTU54_001205"
     CDS             31490..32263
                     /gene="sufC"
                     /locus_tag="LTU54_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBL8295928.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32276..33562
                     /gene="sufD"
                     /locus_tag="LTU54_001206"
     CDS             32276..33562
                     /gene="sufD"
                     /locus_tag="LTU54_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBL8295929.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33559..34794
                     /locus_tag="LTU54_001207"
     CDS             33559..34794
                     /locus_tag="LTU54_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL8295930.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34781..35251
                     /locus_tag="LTU54_001208"
     CDS             34781..35251
                     /locus_tag="LTU54_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HBL8295931.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35256..36647
                     /gene="sufB"
                     /locus_tag="LTU54_001209"
     CDS             35256..36647
                     /gene="sufB"
                     /locus_tag="LTU54_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBL8295932.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36745..37887)
                     /locus_tag="LTU54_001210"
     CDS             complement(36745..37887)
                     /locus_tag="LTU54_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8295933.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT        12329 a         6988 c         8071 g        10550 t
ORIGIN      
        1 taaattaggt aatactttaa gtaaacgctt tctaatttgt tacgggagga atacacttgt
       61 taaaaaaacg tataaaatac agtgcgctaa tgatgaatct ccttatgctc agtaccccgg
      121 ttttaatttc tgtcgttgaa gttgctgcta acgaacaaca gtctgtgaac gaagaaaatg
      181 cacacgtgaa cattgaaggg atgtcaacaa tggaggaaat accaagaact agtgaagata
      241 tggatacgga agaaggaaat gatcctatga tgaaagaatc agaaaatgaa aagacaaaaa
      301 agcaatctga agaagcaaat gaagagtgga aaggggttgt tttcggagaa agcacaagtg
      361 ctagtaggta tgccattgaa ccattagaag acggcgtgcg gctctcttcc acgaataacg
      421 gcggaaaatt ccagtcaagc ggatcagatg gaatgaccta ttattatcaa gcaatcccca
      481 aagacatcaa ctttaggatg cgggcgacga tcgaaataga atcttggacc tatacgaatg
      541 cccaagaagg atttgcttta atggtaagag acgcagtacc aaaaaatcat ttgtttgggc
      601 aggcatttta ttcaaattca tttgccatac taggcagtcg gatcgaatat gtctgggact
      661 cggatagaca agaagtggtc aacacgagcg gcagcaaata tacgatgcga cttggattgg
      721 ggacacgggc aatcacagga atatcatctg aagatccgca agctgcgcca gctcctggga
      781 gtgtctcagt ggaaacaaca cctttagaga cttcagcagg gttcttggaa aaagaaacag
      841 gttcttataa tatattcgga aacggtcatg ggaatctgtt tccggccacg aactcgatta
      901 ctaaactgga cgtagagatg aaaaaaacga atactggttt agaggcatat tattacgatc
      961 ctgaaacagg agaagaaatg gcttctgaca tcatgtatga ctgggaaaaa ttatacgctt
     1021 cagatgaatc ggttatttat gcaggatttg ctgcagcaag gaatatgacg atcaaggtag
     1081 aaggaatcga agttgcttac tcagatcctg cgacagatcc tcctgggcaa gaaagaccta
     1141 ctcgcttgat tgatccgatt tacactgtga cctctagtaa tcattcagga aaccaagatc
     1201 atactatgtc ctttagagca aatgctgacg gattattgac aattaaaaat aaagcaacag
     1261 gagaggtgct taagaacgca aaaaacttgg ctatcactgc gaatcgggaa tttgattatc
     1321 agacaaaact gatggaagga acgaacgaat ttactttcag ttttacgcca gataaaaact
     1381 atccgccaga agaatacgta gagatgacta gttatgaaac aaaagagatt cttcatacag
     1441 tggactaccg taaacctaaa tattcgacag tttatgtaac accagaaggg tcagatgcag
     1501 gaaacggaag cagacaaaat ccattatctc tgacacaagc attgagatac gcatatgctg
     1561 ggcagacaat cgtcatggcg cctggaactt attccttcga aagaggattg acgattccaa
     1621 aaggcgttaa cgggacaaga gaaaacccga ttcagttgat tgcagagaaa aatccagaaa
     1681 acaagcgtcc tgtacttgat tttaaaggaa ctggaaatgg gatgacactg ttcagtcact
     1741 actggggttt acgagggttt gacattacaa atagtgcagc tatgcaaaaa ggattacaga
     1801 tttctggaaa tcataatttg atcgaagaga ttcatgctta tcggaatgga aatacaggaa
     1861 tccaaatcag cggtagctca aatgatcctt tcgaagcatg gcccgcgcat aacttagtca
     1921 agaactgtac atcttttgaa aatgccgatc caggatttga agatgccgat ggatttgcgg
     1981 ccaaactgac aataggagaa ggaaatgtat ttgacggttg tatcgcgtat cataatgcag
     2041 atgacggttg ggaccttttt gctaaaaatg aaacaggttc gatcggaaaa gtaatcatca
     2101 aaaactcagt tgcttatcga aacggctggg taccaggaat tgagggagaa ggtaacggta
     2161 atggattcaa aatgggcggt tcttctttaa cgggccctca tgaattgatc aatagtttgt
     2221 ctttcgaaaa tcttgcgaaa ggaatcgatt ccaatagcgg aacagatatt atcgtcaata
     2281 gcagtacaag ttttaataac ggttcatata atgtggctct ttatacctct tcagcaggaa
     2341 acacagacta ccatgcttct ggtatcttat ctttccgaac agagaatctt gagatgagag
     2401 agcagatccg cccattgaat caaaacgaag atcaaatcta ccactcgttg aactactatt
     2461 ggaatgaacg agagaaacaa tcagaaaata ctcttggaca taccatatca aaagattgga
     2521 tcgagtctct aaatacatgt tcgaaagaag gtcagcagcc tgttacgcgc tatgaaaatg
     2581 gaagtatcca tgttcatgga ctgatgcaga ttaaaccggg gaatcgtcaa acagaaaagg
     2641 aaagagtgga tggtcttcca ttatctgtag gtgctatttt tgacggagaa acctcaccgt
     2701 cttcggaata tccagagtat gcaatcgtac aagaaccaaa tcagacaaaa ccaaatgaac
     2761 aaaataatcc gattattata caagaagcaa atggtgtggc ggagaatgca gaaggaaata
     2821 tcattgaact gaagcctttt acagtttttg tgtcaacttt cacaccaaat caaggaacgc
     2881 ctgtctttga agatatgcgt tatttcccag aaaagatcca aatcgttgaa acacagtcaa
     2941 gtgctcaatt ttctaatcat accgctattt tttcagcacc ttcaaaagga gaatatacgc
     3001 tagaagtcac ttatcgttcg gaaatatttg atggggaaaa atggtctgct catacagaat
     3061 ctgttaccca gcaaaaagcg atcactgtgc gagaaactgc cgatgtgccc ccgggaaatg
     3121 acaaaccaga aagagaaaat ccaggaaatg aatcaccaaa tggaaaacca aatggaaaac
     3181 cttctcatcc ggataggaca gcaaaagaaa gtgaaaaaaa tggcactcaa ggactcccaa
     3241 aaacgggaga atttaataac ccgttgctag cattagctgg aggaattttg ttaatcggtg
     3301 tagttgttta tgtcatgaaa cagcgaaaaa aataagtgga atttctcctt ttatgatgta
     3361 aaacaattga ctgaggaaag taaaaataga aggaaatgag accaatagat gtctcacttc
     3421 cttctatctt tttatagatg aggttctcct tgctgctttt gattggctgt tcttattact
     3481 tgatgcagga cagctttttc acaatctctc cactaaacgg tgttcaatca gctgaccctc
     3541 ggtattagct caacaaacgg caaaatatga ggaactattt ttccgttttt ttctctaata
     3601 ctcaggtcag gacgctgatt tcacaacctc tcgataaacg gtgttcaaaa gccagtttct
     3661 cggtagtaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttac
     3721 tacttgaaac taagcagctt tttcacaacc tctaatacat ggtattccag aagtaacttc
     3781 ttcgaaaaat aagccgaaat gtcacaaaaa tttgagaagc gattttccca aattccttct
     3841 tatttcttga agctgaccac ttctggcaca acctcttacc agattcgcac tcttttttct
     3901 ggcgctagat acatagcgtc ttgttccgtg ataccaaatg tttcatagaa ttcgtcaagg
     3961 ttttttggtt gcacgtttgc tcggagttta gcaggggcat ggacatcgat ttgcagtaaa
     4021 agttgttggt attctttttt tgcttttgta cgccaaatcg ttgcccaatt gacaaagaat
     4081 tcctcgatag aaagatctga ttccgttttt gctgcttcca atgcacagct tagaccgcct
     4141 gcatcagcaa tattttccga aacggttaat tttccgttta cttttccgtc agcaaaaggc
     4201 agaccatcga attcttcgat cattgcttgt gctttgtttt gaaattgtgt aagatcttct
     4261 tctgtccacc agttgttcaa gttgccatat tcgtcaaata aagcgccgtt attatcaaat
     4321 gcatgggaaa tctcatgagc gataacagct ccgattccac cataattagc agagcttgat
     4381 tgttctagac tgtaaaatgg tgcttgaagg atcgctgccg gaaagacgat cacattccga
     4441 aatggatgat aataagcatt gaccgtgtca gcactcattt cccattctga acgatccact
     4501 ggtttgttcc atttgctgaa acgatctttt aatgtgagtt tgctgaaaac aagagtattt
     4561 gaaagcaaag taccaccctg atctgcaggt actgttttga actggtcgaa taatgctggg
     4621 atcttatcgg ggtagcctac ttggatacct aagttattta attttatgac ggctttttct
     4681 tttgttgcat ccccaagcca gtcattttct tctagacgtt ttttataaac ggcgatcatt
     4741 tttttcacca tttgttctac gtctttttta gcagcttcgc caaaatattt ttttccgtag
     4801 taatcgccaa ctacttgatc gaattgtcca gaggcaaggt aatacgctgc ttttttctga
     4861 ggcattgctt cgtctgttcc agacaatacg cgagaataga ttccgctgac ttggcggaat
     4921 tcttctgata gataaccact aagagaacgt actgttttaa tgatcatcca gcttttgaat
     4981 aacggaaaat gagcttctgt caggatgtca ttcaaatgat cgaagtacac tggatcagtt
     5041 acgatcactt tatctggagt tgtcccaatc aaacccgtga caagtttttt caagtccagt
     5101 ttatctgtgt atttaataaa tgtatcaaaa gattgcggat tatacatttt gctgtaatct
     5161 gcgttttctt ctgcactttt tacatgagga gcaatcaatt tatcaaattg cactgcttgt
     5221 tccgtgattg cttctgcctg acttttttct ttgccggcaa gctctaataa ctgaacagcc
     5281 atatcaaaga aaacgcctat tagttgtgca ccgttgggat ggtctgatgc ataatacgtc
     5341 ttatctggaa ggaataaaga aggcggataa gcaaagaagg cgttcgtttg cgcattcttc
     5401 atatctgcat ctacatccag attaaatggc aatggtaatg tatccaacag ccattctgga
     5461 aattgcttat tcaattcgtc gaaagactgc aatgattcaa ctttttcgat tataggttga
     5521 agcggttgag catgcaactc atttcttcgg tcataatcgt ttgccagttg ataatatgca
     5581 aggaaatgtt tcattttttc tgaagacact ttttctggat gggcaagcag atcgtctgtt
     5641 tctcccatca aaagttggtc gatcccgtca actaaatctt ggaaaccgcc tgtagctggt
     5701 ttatctgcag ggatttttgc tgttttttgc cattcttcat tgactgcttc atagaaatct
     5761 tgtttcaatc gttctttgtt cattcatcga tcgctccttt tttgtttcgt gtgcccatta
     5821 tcttacaaaa tgaaggaatt aacaaataga acataaaaaa ttgaaaattc gaacaatcaa
     5881 actgatttgt ttttttcaat ccttcactta taatgaaaaa aaggaggcga tcccaactga
     5941 atcaagtagt ggataaacct ttatatttca aagtagataa aaaaattaaa aaattagcca
     6001 gcactcagca gctgcagtca agaaaaagta aattgctttt tttagctctg gtttttgaag
     6061 atcaaagtta tgtgattatc gatcaatcag gacatccggt agaatattct ccagcaaaat
     6121 atacttatca agagggtctt tcttgtaaaa aatggaagct gatcaatgaa gagccaatcg
     6181 agttatctag atggatcaac agaaaagaag atattcccgt tttgatcgaa gaaaaaaggt
     6241 caggaaagga actcgcaaat tgctgggtgg gattgccaga agaacgtttt ttgaggtata
     6301 agaaatgggc taccccctca ggctatcttt gtggtactta cgcggcagca gttctccttg
     6361 cctattatca agactatcgt aaggggtgga tgcttccaaa tgagattcgc aagaaaaata
     6421 cggctgattc gttggttttg acgaaggcat tgcgtagcca gatccaacca ctaggacttc
     6481 ctacgattcc ttttcaggtt agtacaggga tttctagttt tttaaagaag aatggcaatc
     6541 atgagcgtgc acgagcaact cttctaggtt catggcagcg agctacaaaa cgtatacgtg
     6601 aaggcaaacc tgtgatgatt ggtattttga aagtacttgg cagtacatat ggcaatcatt
     6661 gggtgacagc gtacgcttat tttgaaactg aaacaggaga acgttattac aaagtacacg
     6721 acaactgggg cgattatcat aaagtcattc ctgccagttg gagtaacgga acagtttcac
     6781 ttccttaaga atttgctgaa attaacagaa aatcttggaa aaatatgtta aactatcgaa
     6841 gaatagagaa gttttgggga ggactacatg agtaataagt atcaggatga cacgttgaag
     6901 atttttagtt tgaatggcaa ccgtccatta gctgaaaaaa tcgcgaaagt gttcggaaca
     6961 gaattaggta aaagcgtcgt gaagcaattt agtgatggcg agatttccat caatatcgaa
     7021 gaaagtattc gaggggatca tgtgtatatc gttcaatcaa cgaatgcacc tgtcaatgat
     7081 tactatatgg aactactgat catgatcgat gcaatgaagc gagcaagtgc gaaaacgatc
     7141 aatgtcgtat tgccttacta tgggtatgct aggcaagacc gcacggcgaa acctcatgaa
     7201 ccgatcactg cgaaattgat cgctaacttg atcgaagaag caggggcaac tcgagtactg
     7261 actttagatc tgcacacggt tcaagtacaa ggattctttg atattcctgt agataattta
     7321 tttacgatgc cgttgtttgc tcattattac cgtcaattag gtctgacggg agatgacatc
     7381 gtagttgttt cgccaaaaaa cagcggtgtt cagcgtgcac gtagtctttc agaatatttg
     7441 aacagcacat tagccatcgt agatcatgca gatgatgctt caactgaggg tggttatgtg
     7501 atcggtgatg ttcaaggaaa gacatgtatc atggtagatg atatcttgaa cacaggagca
     7561 acgcttgcac gagcagcaaa tgtattaaaa gaaaacggcg caaaagaagt atatgcctgt
     7621 gcgtcacatg gattattgtc cgatcatgcg aaaagtattt tagacaatgc accaatcaaa
     7681 gatatttgta tcactgattc tgttttcaca gaagaaaatc gtcatccaga aaacttaaca
     7741 atcgttacat gttcagaatt aatgggtgaa gcggtcaaac gcatccatga aaatacaccg
     7801 atgagtccgt tattccgtct ggaagaaaaa gaattcgaat aaacaaaaaa aagagggaaa
     7861 gaagttgtga cagaagtgag cagcttcaag gagctgcgac aagacttttg tcacagctcc
     7921 ttttttctat gcccatgaaa cgtcctctag acaaaaatga gctcttcttt ggtttgttcg
     7981 ctattttatg ctagaatgtg cctagcaata atttggaagg gatgaatgaa gtgaaaacta
     8041 tttatctgga tcatgcggca actaccccca tgcatccaca agtaattgaa gcgatgacgg
     8101 caatcatgca agacacgttt ggaaatcctt caagcataca tggatttggt cgccacgcgc
     8161 atgaaaaatt ggaaacagca agacaaatca ttgcagacag tctgcaagca aaacctcacg
     8221 agatcatctt taacagtggg ggaacagaag gggataatac agctatctta gaaacagcgt
     8281 tctcgcgtca aaaagaaggc agacatctaa tcactacagc tatcgaacat cctgctgttc
     8341 ttaataccat gaaatattta gagacaaagg ggtttgacgt tacatatctg cctgtagatg
     8401 aaaaagggaa tctgtcgatc gaagaggtca aaaaagcgtt acgagaagat acgatcttgg
     8461 tctctgtgat gtacgggaac aatgaaatcg gaaatttgat gccaatcaaa gaaatcggtg
     8521 aacttttagc ggatcatcca gcttatttcc atacagatgc ggtacaagcg tacggaaacc
     8581 aaacgatcca tccagaagaa ttaggaatcg atttattaag tatctctgct cataaaatca
     8641 atgggccaaa aggcgtcggt ttcttatata aacgagacgg agtaaatatc cctgcattgc
     8701 ttttaggcgg ggagcaggaa gaaaagcggc gtgcaggtac agaaaattta gcagggatct
     8761 ggggtatggc aaaagcagtt gaactgctgt cacctgaaga acgtgaagca cgtaacaaca
     8821 aatataaagg attccagcaa ttgattcttg atacattaga agcaaacgaa atcccttatc
     8881 aagtaaatgg tgatttaacg aataaactcc ctcatgtgtt gaatcttcgc ttaattggcg
     8941 aagcaaatga tttgctactg atgaagcttg atcttaatgg ggtcgctatt tctactggtt
     9001 cagcttgtac agcaggaaat atcgagcctt cacacgtatt agaagcaatg tatggagaag
     9061 atacaccgat actaaaagaa tccatccgta tcagctttgg tttgggaaat accgaagacg
     9121 acatccagta tttccttgat caattagttc gtgctgtcaa aaaataacta gtgaagtcat
     9181 tcttatatga acagatgtct gagagaacgc cttcattttt tagggaactt aactgaaaaa
     9241 cctagtaaaa atcaggataa acttttataa taaagagaaa ctaattttat cgaggtgaaa
     9301 atcatggcat ttgaaacaac agcaacggta ctgggcgcac caattagcta tcgtttgcat
     9361 cctgatgcaa aaagatatac attgcgcgat aatggattta cagaaacaaa cggaggcaat
     9421 tatcaattga tccgaccatt agatgccacg ccgcaaagca aagaaggatt caaattaaaa
     9481 atcactgtat caaaagatat tcaaacattg aaaatgtcga tcactacagc aaatggattg
     9541 aaagcagtca atattttcaa agatccaaaa cagaaaatga gccaagataa attttatttt
     9601 ctgatggatg ggatgattag ccgtgggtta ttcgaaaaag tcgaatgatc tgtggatata
     9661 aaaagacaac tagttttgaa acggcctatt gttagatgac taactttgta aggacgacgc
     9721 aaattagttg tcttttttat ttttaaattt aacacatgtt gttattttta atattttgct
     9781 gatttattgt ttctaagtaa agaaatttaa attgaaacga ttgttattta gggaaaaagg
     9841 tattttttga aaaaagaatc ccgtataatg attgttgtgc aaacgctata caaaatgaaa
     9901 aagaaaagag ggatgtttat ggaaaatgaa cagtcggtcg ttttaacgaa ttggaagaga
     9961 aattatttgt tctttttatc gggacagttt ttgtcaggaa tcactagtat ggtcgtccaa
    10021 tatgcgatca tctggtattt gacaagagaa accggttcgg cgacaatttt gagttttgct
    10081 actttactag gaatgattcc catggtttta ctaagcccat ttgtaggacc gctagtggat
    10141 cgctgggata aaaaagctct tttgattgtc acggatatta ttgtagcgat tttcgcgctt
    10201 attttagcag tagtcggaac gatttcagaa tccttcccga tttggctagt ttttgtgtct
    10261 ttgtttatgc gttcggtcgc acaaacattc caaatgccaa cgatccagtc gattatgccg
    10321 acaatcgttc catcttctca cataacaagg acgaatggac agttaggaat ggtccaatca
    10381 gcgaatttca tcattgcgcc agctctaggt gcggctttgt tttcagtcgt tcctgtcaat
    10441 taccttattt tattagatgt attgggggca gtttttggtg tcggcttatt gatttttgtg
    10501 aagatcccaa aagtttctcc tgaaatattg gaagttccgc ttactatttt taaagatgca
    10561 aaatttgggc tgcagcaatt gatggataat aaagggttat ggtacataac gatcaatggt
    10621 gcatttgtga tgctgttgtt catgccagca atcagtctgt atccattgat gacgctagat
    10681 tactttggtg gttccgttgg acaggcggga gcagtggaag tagtctacgc agtcggaatg
    10741 ctcttaggcg gtgcactgat cagttttata ggaacatgga aagaccgaat gaaacctatt
    10801 attatagcct acatcattat gggcctgacg atcggtgcaa gcggattggt tccaaacgat
    10861 agtcaagggt tcttgtactt tcttatcttg aatgcaggag caggttgtgc aacgccatat
    10921 ttcaatacac tgctgatggc aatgatccag cagagttatg aatcgaatgt tttaggtcgt
    10981 gtcttaggga acttcaactc tttgatgaac ctggcaggtc caattggatt attatttgca
    11041 ggaccattag ctgatcgttt aggggtagaa aaaatgttct tgttttccgg aatcgggatt
    11101 ttattatgcg gaatcgtttt atttttaacg tcagctgccc gaaattatga taaagaatta
    11161 cagaaaaaac tggtaaaaga acaccatgag caaaaagacg aatagtatgg aatggagttg
    11221 tgacagaagt cttgttacaa ctccatttct gatcctaatg ttcctgtggc aataagccta
    11281 ctcaaatgaa aaatatgagt aattatcttt atttgtttaa tacttgatat agggcagttt
    11341 tttcacgacc tatcgtactt cctctttgct tcatagattt ctttgatttg ttgctcagat
    11401 aacggagtgc caaatggtag tttttccttt aagagttctc tttccggtgc gtctacgcct
    11461 acattgacat cgttctcgat aggtacgctg taatgatgga tatgtccatg aagattgcgg
    11521 atagtgtcgt taggcccgag caacatcgga taatgagtca agtaatattg ttgatggtcg
    11581 aatttaacga tggcacctac atcataaaaa taaaatttgg gacttccgtc agtaagccta
    11641 ggatcatttt tttctaaata acggaaaaaa gcgcgactgt catggttccc tttgatcaag
    11701 tggattgttc cttgcagttg agtgaccagt tccaaaattt ctgcatttgc tttttcatat
    11761 tgcggatgca tagcaaggtc gcctagatga taaacatggt cggtttcttt tattactgca
    11821 ttccagctag tgatcaactg ttggttcatt tcatccacat ttgcaaagag ccgcggagca
    11881 aagtcgtttt ttcctaataa gcgttcatgg aaaaagtgca tatcactgat aaagtaattc
    11941 atgtatcttt tgcctccctt ttttcttaga gtaaacaaaa tcacttatct gagcaactca
    12001 taacatctaa aaagaaaagg ttgtaaatga gacgtgcagc tttaagcgat aagaagcaat
    12061 tttgaaaaat agtttttcac aatttataaa attattcctt attgccgaaa ggctggccct
    12121 ttttatgagt atgccaattt tttatatcga atgctcaaaa gatgagtgat tttttggttg
    12181 actatcatgt ttgtgtactg aataggtcca gtagaacagt atttttacga cttgataatt
    12241 aagggatgaa attttcagaa atatgacgta taatgtaaaa tactgaaaag ttgcgacctt
    12301 caactcgtag atgtttcaga atctaattga aatgatggtg atatcatgac agacaacagc
    12361 aaaacacgcg ttgtcgtagg gatgagcggc ggtgtcgact catctgtaac agcacttttg
    12421 ttaaaggaac aaggatacga cgtgatcgga atattcatga aaaattggga tgacacggac
    12481 gaaaacggtg tatgcacggc cacggaagat tataaggatg tagcgaaagt agctgctcaa
    12541 atcgggattc cttattactc tgtgaatttt gaaaaagaat attgggatcg tgtatttgaa
    12601 tatttcctag ccgaataccg cgcaggacgt acacctaacc cagacgtgat gtgcaacaaa
    12661 gaaatcaaat tcaaagcatt cttggattat gcaatggatc ttggcgccga ttatgtagct
    12721 actggacatt atgcgcaagt cactcgcgat gaaaacggcg tggtccacat gcttcgtgga
    12781 gtagacaata ataaagatca aacttacttc cttagccaat tatcacaaga acagctttca
    12841 aaaacgatgt tccctctagg cggcatggaa aaatcagaag tacgggcaat tgctgaacgc
    12901 gcaggccttg cgactgctaa gaaaaaagat tcaacgggaa tctgctttat tggtgaaaag
    12961 aacttcaaac aatttttgag caactatctg ccagcaaaaa aaggaaatat ggtcactctt
    13021 gatggtgaag tcaaaggaca acatgctggc ttgatgtatt atacgattgg tcaacgtcaa
    13081 ggactaggga tcggtggcgg aggagattcg caagaaccct ggtttgttgt aggaaaagat
    13141 ctagcgacaa acacattata tgtggggcaa gggttccatc atcctgcatt gtatgccaca
    13201 agcttggatg caagtgaaat ccactttacg acgaatgaac caatgccaaa agaattcaag
    13261 tgtacagcga aattccgtta tcgtcagcaa gacgttcctg tgacagttcg cttattggat
    13321 gataatcgtg cagaagtcgt attcgacgaa cctgtacgtg caatcactcc tggacaagca
    13381 gttgtatttt atgatggaat ggaatgtctg ggtggtggtt tgatcgacca cgcatatcaa
    13441 gaaacaaaag ttttacaata cgtttaagcg atattaaatg aatcaaaaca gctatggcta
    13501 acaaaagaaa caccttttct tttggatagc atagctgttt ttttgcaata aaattaattt
    13561 ttattcattt tatacttgaa atacttgtgt aaaaagttca taatatccaa taatatgaat
    13621 aaagttgctt tttgttaagc aagagcagtt gcaaaataga agggaatctt ttgcaagtga
    13681 tttgttcaat tttataagtg agggtgatgg aaatggaaaa gaaatcgacg ggtattagca
    13741 aatggggact ggttgctctg gttgtcagtt cgtcaatcgg aagcggtgtg tttgggatca
    13801 caagtgatct ggctagttct gcagctccag ggccagcgat cctttcatgg ataatcgttg
    13861 ggattggtat tttagctttg gtattatcgc tgaatcattt aggagaaaag cgtccagatc
    13921 tagatggtgg tattttcggt tacgcggaag cttcatttgg taaattaggt ggcttcatca
    13981 gtggttgggg ttactggttg tctgcgtggc tgggaaatgt tgcttttgca acgatgctga
    14041 tgagcgccat gggagaattt ttcccgatat tcaaaggtgg acaaaatgtt ccttctattt
    14101 tatttgcaag tatttttatt tggggtctga ctttattagt aaacaatgga gttgaaggag
    14161 cgagcgtcat caatacaatc gtgacgatct gtaaattagt gccattattc ctatttttaa
    14221 tatttgtatt tatcgcattt aaagtgaata tattcactgc tgatttttgg ggtaacgtat
    14281 ctgataatct agtgaatgga acagggcagc aaggaacgat ctggttgcag attaaaggtt
    14341 gtttgatggt tatgatgtgg gtctttgtag ggatcgaagg cgcaagtgtt ttagccaacc
    14401 gcgcgaagaa acgttcagaa gcccagcaag catcgatcat tggtctgctt tgtttattgt
    14461 ttatctatat ccttgcttcg atcttgccat acggcgtctt atctcaagaa gagttagcaa
    14521 ctatttcaca acctgctatg gcaaatattt taaaaggaat cgtgggaaat tggggagcag
    14581 cgctgatcaa tatcggcttg attatttcga tcattggttc ttggttgtct tggacgatgc
    14641 tcccagcaga aacaacgatg ttgatggctc gagacggtct gttgccgaaa ggttggggaa
    14701 aaacgaataa aaaaaatgcc ccaacttatt cgttagttgt tactgcaatc ttgactaatc
    14761 tattcttatt gactttctta gttactgatt atgcctatca atttgcctat tctttatgta
    14821 cagcggcaat tttgatttgt tatctacttg tcgggatcta tcaagtccag ttttctttaa
    14881 aagaaggaat caaaaaacaa gtagtgatcg gtgggattgc tgtagttttt gaattgatgg
    14941 gcatcatttt agcaggattt tcttatgtcc ttttatgtag tattgcctat cttcctggtt
    15001 tttgcttcta ctggatcgct tgtcgtgaaa ccaaacatca aattacggga aaagaaaaag
    15061 taatgattgg gctgatttgt gtggccgcac tgctttcaat cgggttgctt gctgtaggct
    15121 ggatcaagat ataaggggga aagaaacatg aaaatcgcaa atttcggtgt agaagaatgg
    15181 ctaaataaat gggaaaagaa agcggtatat gatattgcac aaagctcgat cgaagcattg
    15241 acattagacg aactcgttgg cttagatggg actagcgtaa gtaaattttt cgaaaaacgc
    15301 aaaaacgaac cattggacta tggctggatc gaaggatcag atgttttcaa acaagaagtc
    15361 gcttctcttt atcaaaccgt cgatccggaa aacatcttac aaacgaatgg agcgactggt
    15421 gctaatcttt tagctctata cgcgttagtg gaagcaggag accatgttgt ttcgatgctt
    15481 cctacttatc agcaattata tgacatcccc aaatctttag gtgccgctgt tgactttgtt
    15541 catttgaagg aagaagaaga ttggcagttt gatttagaac agctagaagc cttagtcaaa
    15601 cctgaaacaa aaatcatctg tttgaatagt gcaaacaatc ctacaggcac attgctagat
    15661 cggaaaactt tagaaaaaat cgcagaaatt gctcgtacag tagatgcata tgtattgata
    15721 gatgaagtat atgcgccttt aacggataaa ggtgaattct tgtcgatcgt agatgtctat
    15781 gaaaaaggta tagcaaccaa ttccctatcc aaaacctact ccattccagg tattcgtatt
    15841 ggctggacag ctactggacc tgagttggca gaagtttttc gaaagtatcg agactacacg
    15901 atgatttgcg gcggagtcct gtcagatgat cttgctgtac atgcattgaa gaataaagaa
    15961 aaaatattgg cgcgaaacaa aaagatcatt acagagaacc ttgtgatttt aaaacaatgg
    16021 gtagccaatg aaccgaaagt tgaacttgtc gctcccaatt acgtctctac ttctttcatt
    16081 aaattagcca tcgaagaaga tgaccagaca ttttgtatca atttattaga agaaacagga
    16141 gtactattag taccaggaag tgcctttgat ttaccgaagc atgcccgact aggttactgt
    16201 tgtaaaaaag aaaccttgga aaagggatta ttcctcttat ctgagttctt gaaaaaacag
    16261 gcctaaacaa aaacactgca aggacagtct gtgacaactg ttcaagcagt gtttttcatt
    16321 tacgcttatt gtctatgaga aggagagtgc cctgaagcta tggagactcc tgaagctttt
    16381 tcttctgcag tttcttgctg atggatctct tgtgtattgt gtcgtacaag caagaagttg
    16441 accaaaacaa gaagagaagt cgaaagaaag acgccgcgat aaccaaatgc actggaaaca
    16501 ctggagccga tcataggccc tactacgtta ccagttgctt ggaaagattg gttataactg
    16561 aagatccgtc cagcagcttg atgaggcgaa tattttgtga tcaatgcttg gacagcaggg
    16621 agcagacaag catcagagat accaatcaaa aaacgtaagg cagctaattg ccaaacattc
    16681 gtgacgaatg ccattgggat ataaaccaga atcgcaaaac ccaaaccaat cgcaagtatt
    16741 ctttcactcc caatcttgtc acctaagcga ccaaatcttg gagcggctat caaagtggca
    16801 atcccaggta tcgaagcaat gattccgctg atcaacgtaa cattcccatg tccatgcagc
    16861 aattggcgaa tgtacagact gatgattgga ctaatggaat tgttggatgc ttggatgatc
    16921 atcgttgtga cgaacatacc gatgacgacg tgtggatatt tcaagtcttt gaaaatctgt
    16981 ttggcagaaa ccatgttttc ttttttcact gggacaaatt tttcatggac gaaaaacaga
    17041 ctcaacaaga agacgaaaaa taagatgatc cctgtaatga aaaaggtagg gcgatagcca
    17101 aacgctgaag cagagatccc gccaagaagc ggtccaagta atgttcctgt aacagaacca
    17161 gttgctaagg ttccaagaac ttgaccactt ttttcttttg gcgtcccagt tgcgacaagg
    17221 gcagtggcat tactgatata accagaaaaa atcccttgaa gcagacgcaa tgcgactaat
    17281 tgatacacac tagtcacgca acccatcaaa gaaatgacga ttgccattcc taatgaagca
    17341 cgcaacagca tcaattttct tcctttttgg tcggctaatt ttccccacca aggagagata
    17401 agcgtagtca ccaagaaagt agatgaaaaa gtcaatccgc tccaaaagtt caattgtgac
    17461 gtactataat tacctaacgt atctatgtaa agtgacataa aaggcatgac taaactaaat
    17521 ccgataccag ccataaatgt tccaaaccat aagacaaaca agttttgttc ccaagtttct
    17581 cttttcctaa ataatttttc ctttattctt gcagccaatt agactccttc tttctgaaaa
    17641 ttttcatcta cagctcttta ttataaacta caatatgtag tttatcaatg aaagtctctt
    17701 ttttctcttt ctttttcgtg gtaaactgaa agagtgaagg atttatttta tgagaggaaa
    17761 catggaaatg aaaaaaagaa atctgtctca agagaaaatc atcgactgct ttcgagaact
    17821 agcagaagag atggatgttc agcaaataac ctttcagcat ttagccaaac gcttggatat
    17881 caaggctccc tcgctgtata accatttcaa aaatattcgt gatgtcaaaa cagcgctaac
    17941 agcgaaactt ctgcaggaac ttaatgacca gctgcgacgg gctcttgtgg gaaaaagtgg
    18001 tcaggaagca ctgcagatct atgcagaaac ttatcgatcc tttgcttttt caaatcaagc
    18061 agtgtatgag ttactgatca gtgtaccgca taccaatgaa gctgttttat tagatggaat
    18121 ccatgaaacg aatcagatca ttttgcagat atttgaggct tttcaattaa cgaaagaaga
    18181 aaaagttcat cgaagcaggg aacttcgtag tatgatccac ggttatcttt ccttacgttt
    18241 tttaggatac tttacaaaag aaccaacaat cgatccagaa gacagctatt tttggatgat
    18301 caataacttt atcgctactt tgccttaaac aatcagattc atttgtaaca attttaagat
    18361 gttcattaaa tgaccctttt ataagagaaa attcttgcaa ctttcttgaa aagtgactaa
    18421 gatgagtcta gaaaagctga aagaaggagc acgagtatgt atcaagtaat taccatgttc
    18481 ggggacaatg agccttggtg gttttttgaa gactgggaag aagatattga agaagaagaa
    18541 acattcgagt ctttcgaaga agcacgacaa gcgtacgaaa aacaatggaa cgaaatccat
    18601 caaaattatg aatatatcaa tgcaaaaagt aactttctca gcgccttttg gaatgacgga
    18661 gatgagcgct ggtgtgaaga gtgtgacgat gatttacagc aatacaaggg acttgcgttg
    18721 ttgaaaaatc atcagcccat cacagtagaa agcagaaagg aattttatga aacaactaat
    18781 tctagcggaa aaaccaagcg ttgcgaaaga cctaagcaag gtgcttggtg ctaatcaaaa
    18841 gcacaaaaat tattatgaag gacccaaggt gatcgttact tgggcccttg gtcatttatt
    18901 aggattgaaa atgccggaag atctgaataa ggaatggcag agctggcaaa tggacacgtt
    18961 gccaatgatc cctaaaaaac taggaatcaa accacttcct aaaacaggac atcaattaaa
    19021 agcaatcaag cagctggctc agcgaaaaga tgtctcagaa gcagtcattg cgacagatgc
    19081 cggaagagaa ggggaacttg ttgctcgctg gatcttagag tatgtgcatt tcaataaacc
    19141 agtgaagcgg ttatggatct cttctcaaac agataaagca atcaaaactg gattcaagca
    19201 actaagacct gcgaaagaat atgatgcatt gtatgaatct gctcttgccc gggcaaaagc
    19261 ggactggctg gtaggattga atgtgaccag agccttaact gtgaagtacg cggataattt
    19321 atcagcaggc cgtgtgcaga caccgacact agcattagtg agagaccaag aaaagaaaat
    19381 cgagcagttc cgtccacaaa cgtatttcac gatcacgttg caagctggaa aagaacaagc
    19441 caaaatgctt caaaaaaatc aatttgcttt aaaaacacaa gaagaagcac aaaaacttgt
    19501 tcaattgatc agtacaagca aaggaactgt cacagaagtc caagaaaaat taaaaacgga
    19561 atctgcgcca ttgccttatg atttaacaga gatccaaaga gaagccaatc aacgctatgg
    19621 ctattctgca aagaaaacat taagtcttgt acaaagtttg tatgagaccc ataagatcgt
    19681 tacttatccg cgtacagaca gcaaatatct gacaaatgac atgaaaagta cgatgaaaga
    19741 acgattgcaa gcagtcagcg actttgctcc tgaggtcaag cagtatttga aaaacggagc
    19801 catcgtccaa cagacaaaag tattccaaga cagtaaagtg accgatcacc atgcactttt
    19861 gccaactgaa aacagagcac gttatgaaaa attaagtaat gaagagcgaa aaatctatca
    19921 aatgattgtc tctcgttttt taggactgtt tgctcaaccc cataaagtca gccaaacaaa
    19981 agttacggtt gaatttggta aagaacgatt catttttcgg caaaatcgtg tcgtccaagc
    20041 aggatggaaa ggcgaaactg aaactgagac ggaaacagtc aaatgggaaa aaggaatgag
    20101 aatcaatccc gatttcgcca tcaaaaaaga actatctgca ccgcctaaac cattgtcaga
    20161 agcaagcttg cttggtcaaa tggaaaaata cagtctggga accccagcta cgcgtgcaga
    20221 gattatcgag aaactaatca aatctgaact gatggaacgt acccctcaag gactgcaagt
    20281 atctcctaaa ggtaagcaat tattagtatt ggtaaatccc tctttagtaa caccagattt
    20341 gacagaaagc tgggaaaaag accttgaagc tattgctgca ggaaagaaac aagcaggaac
    20401 cttcttgaat ggaatcgaaa aagaaacaaa acgcttggtg aatgagatca aatcaagcaa
    20461 acaagaatat caagatttct cgattaccca aaagaaatgc ccagaatgtg gggccaatct
    20521 gcgcgagaaa aacacacgtg acggcaaaat ctatgtctgc accaattcag attgcaacta
    20581 ccgcagaaga aaagatccaa aagtctctaa tcatcgatgc ccgcaatgtc accggaaaat
    20641 ggaaatcatc gaaggtaaaa acggtgctta tttccgttgt aaatacgatg gaacgacaga
    20701 aaaattgctt gataaaaaag agcagaagaa gaaaatgacc aaacatgaag aacgacgctt
    20761 gctgaaaaaa tatgcacaag cagaagaacc agaagaaagc ccattagcag cagcactaaa
    20821 agcagcaatg aagaagtaat aagatatgaa agaggttgtg atagaagtgg tcagcttcaa
    20881 gaaataagaa ggaatttacg aaaattgctt ctcaaatttt tgtgaaattt cggcttattt
    20941 ccgaagaagc tacttctgga acaccattta ttcggaaaaa aggactgtga caagactttt
    21001 gtcacagtcc tttttcattt aaaaaaatgt ttacttcatt tgacgtatat tattggtgag
    21061 gcttcaattc atttggcttc actttttctg tttctcttat gatataaaca ggacgctgtt
    21121 tcgtttctaa gaagattttc ccaatatatt tcccgatgat tccaagacat aacaattgga
    21181 ttccaccaat gaacaggaag atcgagacca tggaaggcca tcctgatgta ggatcaccaa
    21241 acaacaatgc ccgcaaaata atgaacaaaa ttgcaatacc agaaccgaca caagacaaag
    21301 cgccgataaa tgaagcaatc gttaacggtg catccgagaa gttcacgata ccatcaatcg
    21361 agtagttgaa taatttccag aaggaccaag atgtttcgcc agccacacgt tcgcggtttt
    21421 cataagaaat atattctgtt ttaaatccga cccagctaaa taatcctttt gaaaatcggt
    21481 tgtattctgt taattctagg acggcatcca ccatttgacg agtcatcaag cggaaatcac
    21541 gagcgccatc gaccatttct gtatcgctga ttttattgat caactggtag aattttttcg
    21601 caaagaaact gcgtatcgca ggctcgcctt cacgagtcga acgccgggtg ccgacacaat
    21661 cgatatcaga agtagtgatc cggttgatca tctcaggaag cagttcgggc ggatcttgca
    21721 gatcaacatc catcacggtc actaaatctc ctgtagaagc ttgcaatcca gcgtacagag
    21781 ccgcttcttt cccaaaattt ctggaaaatg agacaaagcg gacataagga taagctgagg
    21841 ctagcttctt gattgctgct aacgtaccgt cttttgatcc gtcgtcaata aacaaatatt
    21901 cgaactcatg ggcacctttg aatttttcaa cctcatcaaa aaaaagagga acagtttttt
    21961 cttcattaaa acatgggaca acaattgata ctttcatcgt ttctatcctt tcaatcgtat
    22021 gtttatatat ggtttacatt caaaatttaa taaaccttaa ataacacctt taaaatttga
    22081 ttttacgtaa aagaacaggt attctttttg taaactaacg tatttagttt accatgtttc
    22141 ctcggtttat caaactttta agaacaattt ttattagttg gcaagtagtt ttggatagag
    22201 aaaaaggagg aagttccagt ctattgacta ggagcaaaca aaatgaaaat acacacacct
    22261 atcggctttt tatggttaga tgcagatgaa tccggattga ctgctgtatc ttttcagcca
    22321 atttcagaaa gcgaatcagc gaatcagcga attttaacgc gcacacaaga acagtttgaa
    22381 gaatattttg cgggaaaaag aaaagaattt gatgttcctt tatctataaa gagaggtacg
    22441 ccttttcagc aaaatgtctg gaaagctttg tcgaccattc cttatggaga aatccgaacg
    22501 tataaagaag tagccgttgc tgttgatagt ccgaaagctg tgcgagcgat tgggcaggca
    22561 aatcgaacca atccattgcc aatcgttatt ccttgtcatc gagtgatcgg gcaaaatgga
    22621 aaactgacag gatatatggg aaacgcaata gatggggttg aaatcaaacg ccaattgttg
    22681 ttgttagaag gatatctgca aaattaaaaa cagttgtgat tcggacattg ttccatgcga
    22741 gaatcacaac tgttttttca tgttcattct aaactttgat cagccaaata aatagtaaag
    22801 ccatcactgt ttggagaagc ccgacagcta atccataaga taagaagcgt gtaccttctt
    22861 tcatgaattt tgcaaagtct aaacgcagac caattgccgc taaagcagtg atctcaaacc
    22921 agctgctgat aaaatgtgcc gattggtcaa taactacagg taaagaaaac aagctgttga
    22981 ccaaacaaaa gatcacaaac cctaatacat accaaggaag cggtaatttt cgttttttct
    23041 gcgtatcagt tggcgtttca aacagagctg gttgtttctt gtgcatgaat ttttcaaaca
    23101 gatagacaac aacaacgagc atcataatac gtagaatctt aaatagcatg gcgaattcga
    23161 caacagaatt gtcaagtaag cttgccccag cgactacttg accaacagat tggagtgtac
    23221 cacccaaaag tgcacttttt tcaatgagat ttccaccaaa caactgtaat ccaagaaaag
    23281 ggagagtcaa catcatcaca gtacccagta gattgactag tgtgatgatc tgtccttttt
    23341 cttcctcttt cgcatggatg gaaggggcga ttgaagcaat agcagaagaa ccacaaacag
    23401 cgtttcctcc agccatcatc aaagaagaag tatcgctgaa ttgcagtttt ttaccaatca
    23461 agtaagcacc gatgatagtg actgacattt gcagtaacac aaaaacaatt ccttttacac
    23521 caatttgagc aatcgtttga gctgtgacag ttgcacctag taaaaaaaca gagaattcta
    23581 atagtttgct ttcagcaaat tttgtgccga ttgctagaac aggctgttta aaaaaagtat
    23641 ttcctaataa aatacccaat aaaatagcaa tagtcgctcc acctaactgt ggcagataaa
    23701 tagcaagaag cctgctgaag gcagcgatgg caaaagaaag cagtaaaccg ggtaagattt
    23761 cgcggaccgc tgaatccttt acagatttta ttggatgtga caatgatttc atttgatcgg
    23821 ttcctttcgt caataaatac taaaagcata ctcgaacata gtagcactaa accagtattt
    23881 tgacaaaaga acaagaggtc gtgacaaaag tcttgtacga cctcttgttt cgaataaacg
    23941 gtgttccaga agtagcttct acggaaataa gccgaaattt cacaaaaatt tgagaagcaa
    24001 ttttcgtaaa ttccttctta tttcttgaag ctgaccactt cttttacgac ctcttggtaa
    24061 acggtgttca aaagcttgca tctgcggtaa taagcccaac cagtacaaaa acatgaacag
    24121 ctgtttttgt ttggccgttc ttattacttg atgcagaaca gctttttcac aacctattga
    24181 atcgattatt aagcgatttt tttagaaaca aattttccat gtttgctatg ttctttaatt
    24241 aagaaacgta cgtaatgagc aagaacgtag aagacaacta cacttgcaaa cagacattcc
    24301 gcaactttta agaaatccaa tacgctacta tagataccta aactgttgaa gtaatgaaca
    24361 gtagtaaata tagctgttgc agagataaca agcgggaatg taaaggctgc atagcttgga
    24421 taaaaacgaa gcttgaacaa acgtggaagc atagacagca caacgaaata caatacttga
    24481 gataaagcga acaatgctac gacaagataa aaattaggtt cagcaaagtt tttaagataa
    24541 cctgttaggc aaagagaacc aggtgctgca acgatcgtaa tcagcggacg tgtcggatga
    24601 gccatatttc ttacgaaaaa gacacgtttg atgatgatcg gcaataagaa cgcatagcag
    24661 atcaatgaaa tccaaaagaa aatttttcca ataagtggga agaagttccc ggcagttaca
    24721 gtgatgaccc caaatccaac gtaaatgata aaccagcttg gataaattgc ttcaattgaa
    24781 agatcagctt tgataacatg gtgataattg aaataaaaga caaggatcac ttggaagata
    24841 acagcgcaca gccaaatgaa tttgacaatt ggtgcaatac tttcatatga aacaaaataa
    24901 gtacaaataa ccatgagtga catcgtaaaa gttggtgaaa cggaggcgat gattggatca
    24961 tgcaacgttt gtttcgtgtg ttcgaataag ataaacaatt taacaaaaat gaatatcata
    25021 agaatcatag agataccgcc agttagattg cctaaagcaa catggtgata gtctttaaac
    25081 agatttccaa gggaagctaa cccaaggatc aacccgcaga tcgggatcgg aataattttc
    25141 aaaaagttct tcatatataa tattctcctt tttatttaat agattttagt accaagatga
    25201 acgaatacat tgtaaaatga ataggaatgt atgattacga gcaagcagac agctgattgt
    25261 cttcatctaa gttactagtt ggatcagcaa ctgttttgtt gtacatttct attatagatg
    25321 gttttttgta tatttaaatc aaatagtttt tatagtaatt atcataaaaa gttatggaag
    25381 tggtgccatg tttaagttat taacgacttt tcgagcagta tatgaaacaa gaaatttttc
    25441 cagagcagca gaagtactgt ttttatctca accagcagtc tcgaatcaaa tcaaacagtt
    25501 ggagcgggaa ttgaatatcg atctttttca acgtaacgga agaaaagaga tgcttccaac
    25561 taagcaagcc gacttattat atgaacggac actggttttg ttagaagaat gggaagaaac
    25621 tcagcaaaaa ttgctcaatg aacaaaatga agaagaagta tgtcggatcg ttgcttccca
    25681 cacctttgct gtctatcttt tgcctcagtt gatgaaacat ctgatcaaaa ggtttcctaa
    25741 agtgcatttt gagatcgaaa tcacgaattc tcatgaatca ttagaacaag tagcgaaaca
    25801 tgaatccgat tttggtttta tcgaaaagcc attagagact tccggcgtac aaagatactc
    25861 tttgatggaa gaccagctag tcatcgctgg ggacccaaaa agcaaattgt ggcttgtaag
    25921 agaagcaaac tctggtgttt ttcattacac gcagcgttat tttgaagaga tgaatatcca
    25981 agataagaag atgatcgtga aaagtaacga tgtaattgtt gcgttgctga aggaaggaat
    26041 cggtcgctcg attttgtcta agcgctctgt tccagaaaat gtgccaatga ctccgttgaa
    26101 tttccatcga tatttttatt tcatccaacg aaccaatctg gtttctgatc agctgctaga
    26161 gattgccgaa gagatccttc gatacaatga aagcacagaa acttctcaca gcgaggagaa
    26221 tctcagctgg tagataattg catgtatcaa aaaaagttct atgatacact aaagataagc
    26281 agtaggcttt tgtactgcat ttgctcaagc aactgcacaa acggaaagtt aatttttccc
    26341 aaatgtctat ggaaagactt tccattatct aactggaccg ctataataat cagcagtatg
    26401 gtttttaaga aaagggatga aacatggaaa aaaaattaaa agcttcgaac gataatcgta
    26461 ttgattatgg cgtgatttta ccagtctttc tgttatgttt gattggtctg ctctcattat
    26521 atgtagcact tagccatgat ccgaacaatc cagcagtctt gaaaggcgta gggatgcaag
    26581 ttgtctggta tttagttgga gcggcagcaa tcgttgtcat catgcatttg aattccaaat
    26641 ggatctggaa actgactcct tacctttatg gtttggggtt agtggtaatg ggactcttgc
    26701 tcaaattcta tgatcagaat ttagctgctt caacagggtc taaaaactgg tttcgattcg
    26761 gtgcgtttac tttccagcca gcagagttga tgaagattgc gtacatcttg atgatggcac
    26821 tgatcgtaac taaacataat acgcaagtaa aagaacggac gatgaaatcg gacttttggc
    26881 tgattgggaa gctcttgatc gtgacaatac cggttttagc attgatcatg gcccaagatg
    26941 actttggaac gatgcttgtc tttcttgcga tctttggcgg gattttcctg atgtctggaa
    27001 tctcttggcg catcatcgca cctgtagtaa tcttggcagt agtcgttgga gcaggaacga
    27061 tcttcttggt tacgactgaa ggcggaagag acttgcttta taaagttgga ttcaaatctt
    27121 atcagtttgc acgtattgat tcatggctgg accctttcca tgatacgtca ggaatgagtt
    27181 atcagccggc ccaaggactt ttggcaattg gtaccggtgg cttgtttggt aaaggattca
    27241 acgtaagcaa tatctatgtg cctgttcgtg agtccgatat gatttttacc gttatcggtg
    27301 aaaacttcgg ctttatcggt ggtgcattcg tcattttctt gtattttatt ttaatctatc
    27361 gtatgattcg ggtgtgtttc gatacgaata atgaatttta cgcctatatc gcaagcggtt
    27421 tgatcatgat gctgctgttc catgtctttg agaatattgg agcaaatatc ggattacttc
    27481 ctttaacagg gatcccattg cctttcatta gtcagggagg atcctcgatt ttagggaaca
    27541 tgatcggtat cgggttgata ttatcgatga gataccaaaa tgaagcacct gcaagatctt
    27601 caaggaggta agaaaattgt acacctttta ctggtacccg aaatgttcaa catgcaaaaa
    27661 agccaaagcg tggttagacc agcatggcgt tagttatcaa accgttgata tgatcaaaga
    27721 agtaccgaag gcctcaacga ttgagacttg gctagaaacg agtcaagtac caatccgtcg
    27781 cctgttcaac acaagcggga tgaaatatcg ggaacttgga ctgaaagatc aagtaggcga
    27841 gtttacggct tcaaaagcga gccaagtctt atcgacagat ggtatgctga tcaagcgacc
    27901 actgatctta cgagaggacc agcttgttgc aattggattc aatgaaaaaa tttatgaagg
    27961 agaactgata taaaatggca ggcaagcatt tgaagaaaaa ggacaacctt tggatcttat
    28021 ttaatggaaa agaatactgt gttggtttga caaatgaagc gcaagaagaa cttggagaca
    28081 tcacgtttgc caatctgcca aaagcaggac aatcgctaaa aaaagatgaa cctctgatcg
    28141 aagttgaagc ggaaaaagcg gtcaatgaat ttgccagccc gctaacgggt acagtttctt
    28201 ctgttaatga taaaatcggt gaagatatca atatcttgaa tgattcagat gaaatgaatg
    28261 cgtggatctt aagtttcaaa gatgttgatc caaaagaatt tgacgaatta taaaatcaga
    28321 aaatcacttg aaaaaagttg acactgattc ttccagttgc tataatcata agcaatccta
    28381 tatgaaacga gaaagctttg aaaagaagag tacatgtcac ggacgtttga agagagccct
    28441 gtttgctgaa aatgggtaac gaaaatgaga tggaagatgg tcttagagca gagtaagcga
    28501 gcgtaagcaa acttactacg ggagtgcccg ttacagcgcg acagtatacg gaaagaagct
    28561 aagtccaagt actggaagag gctgttattg cgaagtaacg gtaaatcaag gtggtaacac
    28621 ggaagttcaa gctttcgtcc ttttgcttaa ggcaagaggg cgaaggcttt tttgtttgaa
    28681 aaattataag agaaagatgt gaatggcatg tcattaatcc aactaacgaa tgtcaaaaag
    28741 acattcaatg ggaaaaacgg aaacatccaa gccgtcaatg acgtttcact aaacgttgaa
    28801 aaaggggata tttatggaat cgtcggctat tctggcgcag gaaaaagtac attagtccgt
    28861 ctcctgaatg gacttgagct tcccacgagc ggcgaagtga tcgtaaacga tcaagatatt
    28921 acaaaattaa aaaacaaaga attacgtaca ttccgaaaga aaatcggtat gatcttccag
    28981 catttcaacc tgttatggtc aagaactgta cttgaaaata tccagctgcc gctggaatta
    29041 gctggcgtac caaagagcaa acgtaaagaa cgggcagaag aactgttgcg tttagtcggg
    29101 ttagaaggac gcggacaagc atatccttca caactttccg gcggacagaa acaacgcgtc
    29161 ggtatcgcac gagcattggc aaatgatcct gagatcctgc tttgtgatga ggctactagc
    29221 gcattggacc cgcaaacaac agaagaagta ttagacttat tgttagcgat caataagaaa
    29281 ttgaatttga ccatcgtctt gatcacacat gaaatgaatg tgatccgcaa aatctgcaac
    29341 aaagtcgctg tgatggagtt ggggaaagtc gtagaagaag gagacgtatt gactgtcttc
    29401 agacaaccga aaaaagaagt aaccaaacgc tttgtacaac aagacatcga accagaagct
    29461 gattcgacag aactattagc tgaactcatc aaagaaaatc caagcggccg tttggcaact
    29521 ttgaccttta gcgaaagaaa tgccaacgag ccagtcattt cccaagcgat tcgacgctat
    29581 aacatcgatg tcaacgttgt gtatggaaag atcaaacaag tgaaagaagg ttcctttggt
    29641 tcattgactg tcttgatgac tggtgaaacc aaagaattag atcaagcaga agtgttcatc
    29701 aaagaacaag gagtaggaat tgaggtgatc catcgtggat aaaagctttt cagaaacata
    29761 tctagatttt aaacaaatca acccggaaac gatgaatcag gctatccttg atacactata
    29821 tatgacatta atctcaatgg tcttagtgac gatcatcgga ttagttttgg gattgatcct
    29881 ttactctatt ggacgcaaaa acaaaccgta tgctcgaatc atttatggaa tcgtatcgat
    29941 catcagtaat attttccggt caacgccatt tatgatcctg atggttttga tcattccatt
    30001 tactaaagca atcgtaggca ctatgctagg tgcgcaagca gccattccag ctcttgtttt
    30061 atccgcagca ccattttatg cccgtctagt agagattgct tttcgtgaag tcagccctgg
    30121 tgtactggaa gcagcagatg caatgggagc aagttactgg gaaatcatct ggaaggtctt
    30181 gatacctgaa agtattcccg cattgatttc tggattaaca gttacaacga tctcgatgat
    30241 cggatttact gcgatggccg gcgcgatcgg cgcaggtgga cttggaggtc ttgcttggca
    30301 ggaaggctac cagcgtggta atctaacagt gacattcgtt gcgactttaa ttattttact
    30361 aatcgtattt atcgttcaag gaatcggtga tttcttaact aagaaaacag acaaaagata
    30421 ataggatata gggggatgta ggatatgaaa aagaaaattt ttggatttgc agcagcgctt
    30481 ttgctgactg taggattagc agcatgcggt aacaacagcg acagcaaaga ttcagcaaat
    30541 aaagcagacg acacgacatt gaaagtcggc gcttcaccga caccgcacgc agagattttg
    30601 gaacatgtga aaccattatt gaaagatgaa ggaatcgatc ttgaaatcgt caaattcgat
    30661 gactatgtat taccgaacca atctttggaa gaaggcgaca ttgatgccaa ttacttccag
    30721 catattcctt atttgaacaa agaaatcaaa gaaaaaggat atgactttgt caatgccggt
    30781 gctgtccaca tcgaaccaat gggcttgtac tctaaaaaag tcaaagatgt ttccgaatta
    30841 aaagatggtg caacaatcat tacttcaaat tctgaatcag actggggacg catcatcaca
    30901 attctacaag atgctggctt agtcaaagtc aaagatggag tagatctgga aacagcaaca
    30961 ttcgatgata ttgaagaaaa ccctaagaac ttgaagttca accatacgat cgacccatca
    31021 cttttagctt ctacgtacca aaatgatgaa ggtgacctag ttgcaatcaa cgcaaacttc
    31081 gcttacggcg cagggttgaa tccattagaa gatgccgtat tgcttgaaaa ggacaactct
    31141 ccttacgtga atattattgc gactcgtaaa ggtgatgaag acagtgacaa aatcaaaaaa
    31201 ctgattgaag tgcttcaccg caaagaagtc caagaatgga tcacagacaa atggggcggc
    31261 tctgttaaac cagtaaaggc tgatgcaaaa taaggtatgg aacaaaaaag atgagaaata
    31321 agtctgtaat ggcttatttc tcatcttttt tatacctatt ctaatttaga aatcaaaatt
    31381 gtttaataat aaataaaaga tatttgattt tagtaaggaa ggtcattgcg cttcattctc
    31441 aattagtcta aaatgagtga gaacgaaaga gaatttgggg gtataatgca tgtctgtctt
    31501 agaaataaaa aatctacatg tgtcgatcga ggaaaaagaa atccttaaag gcgtaaacct
    31561 aacaatgaaa accggagaaa tccatgccat catgggacct aacggcacag gaaaatctac
    31621 cttatctgcc gctatcatgg gcaatccaaa ctatgaagta acagaaggcg aaattttgtt
    31681 tgacgggaaa aatgttttag acttggaagt agatgaacgt gcgcgacttg gcttattttt
    31741 agctatgcaa tatccaagtg agataccagg tatcacgaat gcggaattca tgcgtgcagc
    31801 gatcaatgcg aaacgtgatg aaaacgacaa aatttctgtt atggcatttt tgaaaaagtt
    31861 agatgaaaaa atggctttac taaatatgcc agaagaaatg gctgaacgtt atctgaacga
    31921 aggtttttct ggcggagaaa agaaacggaa tgagatcctg caattgttga tgttagaacc
    31981 tacatttgca attttagacg aaatcgattc aggtctggat atcgatgctt tgaaagtcgt
    32041 tgctaaagga gtaaatgaaa tgcgcggaga caatttcggt gcattgatca tcacccacta
    32101 tcaacgatta ttgaactata tcacacctga tgtcgttcac atcatgatgg aaggccgagt
    32161 agtcatgaca gggaatgctg acctagctaa acgtctggaa gcagaaggct acgctggtat
    32221 cagtaaagaa ttaggaatcg actataaaga agaagaagcg taagggggag caagaatgaa
    32281 agaaaaaaac tggctcgatt atcttgacgc ggtaaatgat ttttctttaa gtaaaggcga
    32341 acctgattgg atgagaacct tccgtcaaga cgcactagcg aaagcagatg aactgccatt
    32401 gccgcatatc gatcgggtga aattccatcg ctggtcgtta tttgatgtga aagaaacaca
    32461 aaccatcagt gaaacaggca cgattccggc atttgatgca atgaaagaca atcctgtgct
    32521 tgtccaacaa ggttcatgga cgatttttga acaactgcct gtggaattag cagaaaaagg
    32581 agtcattttt accgacttgt tcactgcaat gatcgaatat ccagaactag tccaagaata
    32641 ttatatgaaa aaagcagtaa acatgaacga agaccaattg actgcactcc atgtagcttt
    32701 tatgaacagc ggtattttcc tgtatgtgcc taaaaatgtc gtgatcgatg aaccactcga
    32761 gtccttattc attcaagatg gcgcatccga tgagcacttc ttcaagcatg tgttgatcgt
    32821 ggcagatgaa catagtgaat tttcttatct ggaacgtttc cagacaacca aagaacaagt
    32881 agcaaaatca tcaggtaata tcatcgtgga agtcatcgct aaagctggtt caaaaatcaa
    32941 atattcagcc gtagatcaat taggagaaaa tattacttct tatatgaatc gccgtgggca
    33001 cattttacga gatgcttctg tcgactgggc aattggtgtc atgaatgacg gtcatgtgat
    33061 cgctgacttt gactcagatt tagcaggaga aggtgcacac gcagaagtca aaatcgtggc
    33121 aatcagctcg ggcagacaaa tccaaggaat cgatacacga gtaaccaata aagcccctca
    33181 tacgatcggg catattttgc aacacggagt gatccgagaa aaaggaacat tgacctttaa
    33241 tggtatcggc cacatcttga aaggggcaaa aggtgcagat gcccaacaag aaagtcgtgt
    33301 cttgatgctt tctgataaag cacgtggtga tgcgaatcct attcttttga tcgatgaaaa
    33361 cgaagtaact gctggacatg ctgccagtgt aggacgagtt gatccagaag aaatgtacta
    33421 tctgatgagt agaggattgc ataaagaaga agcagagcgc ttagtgattc gaggattcct
    33481 cggttctgtc ttaacagcaa tccctgtaga acaagttcgg aaagaattag tcgaagtaat
    33541 cgaagggaag ttgaatggat gatcgatgct aaaaagctac gcaatgattt tcctatctta
    33601 tttcaagacg tcaatgatga acctttagtt tatctggata atgcagcgac gacccaaaag
    33661 ccaaaacaag tactccaggc attagcgtac tattacgaac acgataacgc caatgttcat
    33721 cgcggtgtcc atacgttagc agaacgcgcg acccatgatt atgaacaagc aagagaaaaa
    33781 gtacgggcgt tcatccatgc aaaagaaaca gcagaagtgc tgtttactcg tggtacgacg
    33841 acaagcttga attgggtagc cagaaccttc ggcgaccgct atatcaaagc aggagacgag
    33901 atagtgctct cttacatgga acatcattct aatgttatcc cgtggcagca gttggcaaaa
    33961 cgaacaggtg ctgtcttgaa atatatcgaa ctaacagaag aaggcttcct ggatatggag
    34021 gatgcagcga aaaagattac ggacaaaacc aaaatcgtct ccattgctca tgtatccaat
    34081 gtcttaggtg tcttcaatcc tgttcaagaa ttagctgaac ttgtccatga aaacggcggg
    34141 gtcatcgtag tagatggtgc tcaatctgcc ccgcacatgg ccattgatgt ccaagcacta
    34201 gactgtgatt tctttgcttt cagcggacac aaaatgtgcg gaccaacagg tattggtgtt
    34261 ttatatggta aacgaaaatg gttagaagaa atggaacctg ttgaatttgg tggcgaaatg
    34321 atcgattttg tcgaattata cgacagtaca tggaaagaac taccttggaa attcgaagca
    34381 gggacaccaa atatcgccgg cgctatcgca ttaggccatg caatcgatta tttgcaaaaa
    34441 ctcggtatgg acaatatcca tcgatatgaa gaagaactag ccgcttatgt gttgccaaaa
    34501 ttacaagcaa ttgatggatt gactgtttat ggtccacaag acccgaattt tcggacagga
    34561 gtcgttgcat tcaatttgga tgggttacat ccccatgatg tcgcgacagc tttagacatg
    34621 gaaggggtag ccgtcagagc aggtcatcat tgtgcgcaac cgctattgaa gtaccttgaa
    34681 gtatcagcga ctgctcgcgc gagcttttat ttctacaaca caacgcaaga cgccgaccgt
    34741 ttagttgaag caatcatggc aacaaaggag tttttccaac atgggactat ctaaattaga
    34801 caatttatac cgtcaagtga tcttggatca ttcttcccat ccgcatcatc gtggaagctt
    34861 gactgactcg acaagcaaga tcgaattgaa caaccccaca tgtggagatg tgatcgaatt
    34921 gcaagtcgat gtaaaagacg gaatcatcaa aaatatcgcc tttgacggca gtggctgctc
    34981 catcagtacg gcaagtgcct cgatgatgac agacgccgtc atcggaaaat caatagaaga
    35041 agcagaacat ttaactgtag actttctaca gcttgtccaa ggaaaagaag tcaaagatcc
    35101 cgaccaatta ggcgatgctg ctatgcttgg tggtgtagct aaatttcccg ctcggatcaa
    35161 atgtgcgaca cttgcctgga aagcactagg aaaagcaatg gaaaacgatg gtaccgcagc
    35221 tttccatcat taccacaagg aagaggagtg agaacatggg cgtaccagaa ttagaagaat
    35281 ataaattcgg gtttcacgat gatgtcgaac cggttttcag tacaggagaa ggcttgacag
    35341 aagaagtgat ccgtgagatg tcaagaatca aaggcgaacc agaatggatg cttgaattcc
    35401 gtctgaaatc tctagaaaca tttaacaaaa tgccaatgca aacatggggc cccgaccttt
    35461 cagacatcga tttcgatgcc atcaaatatt accaaaaacc aagtgacaaa cccgctcgtg
    35521 actgggaaga cgtaccagaa aaaatcaaag atacatttga aaagattggt ataccagaag
    35581 ctgaacgagc ttatctagcc ggtgcttccg cacaatatga atcagaagtc gtgtaccata
    35641 atatgaaaga tgaattcgaa aagttaggaa tcgtctttac tgacactgac tctgctttga
    35701 aagaataccc tgaattattc aaagagtact tttcaaaact tgtaccgcca accgacaaca
    35761 aattagcagc gctgaattca gccgtatggt caggtgggac ctttatttac gtgccaaaag
    35821 gcgtcaaagt cgatgttcca ttacaaacgt acttccggat caatgcggaa aacacggggc
    35881 aattcgaacg tacgctgatc attgttgacg aaggagcaag catccactat gtcgaaggtt
    35941 gtacggcacc tacttattca agtaacagcc tgcatgccgc catcgtagaa atctttacaa
    36001 gaaaagatgc gtattgccgt tacacaacga tccaaaactg gtcagacaat gtgtacaact
    36061 tagtaaccaa acgagcaaaa gcatacgaag gcgcaaccgt tgaatggatc gacggaaacc
    36121 taggtgcaaa aacaacgatg aaatacccaa gtgtttactt agatggaaaa ggtgcacgag
    36181 gcaccatgct ctccatcgcc tttgcaggag caaaccaaat ccaagatacg ggtgcaaaaa
    36241 tgatccacaa tgcaccaaat acatctagct ctatcgtctc taaatcaatt tcaaaagacg
    36301 gcggagaagt aaactatcga ggacaagtaa cctttgccaa aaacagtgcg ggttcgatct
    36361 ctcatatcga atgtgacacg atcatcatgg acgaatggtc gaaatccgat acgatcccat
    36421 tcaatgagat ccacaacagc caagtctctt tagaacacga agccaaagtc tcaaaaatct
    36481 cagaagaaca actctactat ctgatgagcc gaggcctgac cgaatctgaa gcaactgaaa
    36541 tgatcgtcat gggctttgtt gaaccattca ccaaagaact tccaatggaa tatgcagtcg
    36601 aactcaatcg attgatcagt tatgagatgg aagggtctgt aggttaataa ctatttcatt
    36661 gctcgaaacg ttgatattaa agcgtttcga gcgttttttt atttgtattt taatggttgg
    36721 ctacgttttt ggctacgttt ggaattattc gcccataaat tttgcaaatc ggtcaccagt
    36781 tttcttaaca gattgtggtg tgacatgagc atagatattc atggtagttt taatatcttt
    36841 atgacctaat cgttcctgta cttcttttat cgacgcacca ctttcaaaca ataagctaca
    36901 gtgggtatgc ctaaatccat gtggagtaat ttgaggctcc atcggtgtct ttttgtaaat
    36961 ccagtctaac caatcgttta ctacttgggg gtagtacaac ttgtttgtgt ttgtagtgaa
    37021 aagaaactgt tttggtgaat tcgtgttata gccatataag atcattattt tcatttgttc
    37081 ttgttgccaa atacgcagtt gtttaagtgt aaaatcatca agctgaatcg ttcttgatga
    37141 atttttagtt ttaggtactt gtagaactac ttgattaaac tcgtcttgag cgatagtttt
    37201 accaattgtt attgttttat tgaaatagtc tatatcttcc cattgaagcg ccaatgcttc
    37261 gcttttacgc attcctgtat aagctaataa acggaaaaaa gtgtacagtt taactgaatt
    37321 attttcttta gtattctgta aaaacagttg taattgatct ttcgtgtaaa acttcaaggg
    37381 ttgttcttct tcaatttttc ttggaagcaa tgttttagac ataggattat ccttacagac
    37441 ttccattgct actccaaatt taaatatttg agcagttgtt tttctcaaga aaccatattg
    37501 ttttaatgtt ttgtgccatt cattcacctg tttttggcaa tagctaacac ttattttatc
    37561 caatcgtaat tccccaaaag caggtaatat atggtttttg caaaaacgtt gagcaacggc
    37621 aacagaagac ggttttactc ttgttctgta gttatcaatc cataattcat acaactcttg
    37681 aaaagttgta acattgattt taaactcttg tttttcttta aagtctacaa gtagtctatt
    37741 aagtgctgtt tgcgcctcag cttttgattt gaatcttcta cgctttgtac gtatatcttt
    37801 tccagtcttg ggatctttcc ctaaataagt ttcaaatccc caatattttt cgccattttg
    37861 taatttataa ctaaaaaatg ttgccatttg attctctcct tacttcatta ttgcaggtag
    37921 agaagttatt tgaagaat
//
LOCUS       DAFGZE010000023        36230 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.023, whole genome shotgun sequence.
ACCESSION   DAFGZE010000023 DAFGZE010000000
VERSION     DAFGZE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.023"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            257..532
                     /locus_tag="LTU54_001211"
     CDS             257..532
                     /locus_tag="LTU54_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295934.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2623
                     /locus_tag="LTU54_001212"
     CDS             545..2623
                     /locus_tag="LTU54_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBL8295935.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            2783..4669
                     /locus_tag="LTU54_001213"
     CDS             2783..4669
                     /locus_tag="LTU54_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL8295936.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4681..5628
                     /locus_tag="LTU54_001214"
     CDS             4681..5628
                     /locus_tag="LTU54_001214"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBL8295937.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5648..6175
                     /locus_tag="LTU54_001215"
     CDS             5648..6175
                     /locus_tag="LTU54_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL8295938.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6238..6891)
                     /locus_tag="LTU54_001216"
     CDS             complement(6238..6891)
                     /locus_tag="LTU54_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBL8295939.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7023..7865
                     /locus_tag="LTU54_001217"
     CDS             7023..7865
                     /locus_tag="LTU54_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL8295940.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8023..8919
                     /locus_tag="LTU54_001218"
     CDS             8023..8919
                     /locus_tag="LTU54_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL8295941.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8922..9635
                     /locus_tag="LTU54_001219"
     CDS             8922..9635
                     /locus_tag="LTU54_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HBL8295942.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9655..10173
                     /gene="msrA"
                     /locus_tag="LTU54_001220"
     CDS             9655..10173
                     /gene="msrA"
                     /locus_tag="LTU54_001220"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL8295943.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQDYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10170..10397
                     /locus_tag="LTU54_001221"
     CDS             10170..10397
                     /locus_tag="LTU54_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HBL8295944.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10549..11100
                     /gene="lepB"
                     /locus_tag="LTU54_001222"
     CDS             10549..11100
                     /gene="lepB"
                     /locus_tag="LTU54_001222"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL8295945.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11183..11683)
                     /locus_tag="LTU54_001223"
     CDS             complement(11183..11683)
                     /locus_tag="LTU54_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL8295946.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11962..12360)
                     /locus_tag="LTU54_001224"
     CDS             complement(11962..12360)
                     /locus_tag="LTU54_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8295947.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12620..13480
                     /gene="ylqF"
                     /locus_tag="LTU54_001225"
     CDS             12620..13480
                     /gene="ylqF"
                     /locus_tag="LTU54_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBL8295948.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13473..14240
                     /locus_tag="LTU54_001226"
     CDS             13473..14240
                     /locus_tag="LTU54_001226"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBL8295949.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14296..15153
                     /gene="dprA"
                     /locus_tag="LTU54_001227"
     CDS             14296..15153
                     /gene="dprA"
                     /locus_tag="LTU54_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBL8295950.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15275..17353
                     /gene="topA"
                     /locus_tag="LTU54_001228"
     CDS             15275..17353
                     /gene="topA"
                     /locus_tag="LTU54_001228"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBL8295951.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17322..18698
                     /gene="trmFO"
                     /locus_tag="LTU54_001229"
     CDS             17322..18698
                     /gene="trmFO"
                     /locus_tag="LTU54_001229"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HBL8295952.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            18896..19801
                     /gene="xerC"
                     /locus_tag="LTU54_001230"
     CDS             18896..19801
                     /gene="xerC"
                     /locus_tag="LTU54_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBL8295953.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            19838..20386
                     /gene="hslV"
                     /locus_tag="LTU54_001231"
     CDS             19838..20386
                     /gene="hslV"
                     /locus_tag="LTU54_001231"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HBL8295954.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            20400..21800
                     /gene="hslU"
                     /locus_tag="LTU54_001232"
     CDS             20400..21800
                     /gene="hslU"
                     /locus_tag="LTU54_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HBL8295955.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            21818..22609
                     /gene="codY"
                     /locus_tag="LTU54_001233"
     CDS             21818..22609
                     /gene="codY"
                     /locus_tag="LTU54_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBL8295956.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            22691..23566
                     /locus_tag="LTU54_001234"
     CDS             22691..23566
                     /locus_tag="LTU54_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBL8295957.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="LTU54_001235"
     CDS             complement(23698..24336)
                     /gene="plsY"
                     /locus_tag="LTU54_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBL8295958.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            24463..24897
                     /locus_tag="LTU54_001236"
     CDS             24463..24897
                     /locus_tag="LTU54_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBL8295959.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            25063..27108
                     /gene="parE"
                     /locus_tag="LTU54_001237"
     CDS             25063..27108
                     /gene="parE"
                     /locus_tag="LTU54_001237"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBL8295960.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            27120..29570
                     /gene="parC"
                     /locus_tag="LTU54_001238"
     CDS             27120..29570
                     /gene="parC"
                     /locus_tag="LTU54_001238"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBL8295961.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDRSIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            29847..32078
                     /gene="pflB"
                     /locus_tag="LTU54_001239"
     CDS             29847..32078
                     /gene="pflB"
                     /locus_tag="LTU54_001239"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBL8295962.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            32322..33083
                     /gene="pflA"
                     /locus_tag="LTU54_001240"
     CDS             32322..33083
                     /gene="pflA"
                     /locus_tag="LTU54_001240"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBL8295963.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            33168..34094
                     /locus_tag="LTU54_001241"
     CDS             33168..34094
                     /locus_tag="LTU54_001241"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HBL8295964.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            34276..35169
                     /locus_tag="LTU54_001242"
     CDS             34276..35169
                     /locus_tag="LTU54_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HBL8295965.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            35303..35428
                     /locus_tag="LTU54_001243"
     CDS             35303..35428
                     /locus_tag="LTU54_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8295966.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(35562..36194)
                     /locus_tag="LTU54_001244"
     CDS             complement(35562..36194)
                     /locus_tag="LTU54_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295967.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
BASE COUNT        12709 a         6210 c         7618 g         9693 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaaacgag tccaataaaa taaaaaggta aaaattttca
     2641 agttttataa aaaacaaaac atatcagaac aggatcgaat cggtcctgtt ctttttttga
     2701 gaacacaaaa agaacaaata ccaaaaaaat tttcctgaag ttcactattt ttatgataca
     2761 atatccaaga taaggaggga acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac
     2821 ttatggtgaa aaagatttgt ttgaccagat ttcttttttg gtccacgaaa aagaccgtat
     2881 cgggttgatt gggacgaacg ggacgggtaa aacaagtctc ttaaatatta ttgccggtat
     2941 cgatagcagc gacggtgatc ggcaaacagt gttttatcca actgattacc gtattggtta
     3001 tctttctcaa gcagctgaat tttccgatga attaactgtt ctccaagcag tctttcaagg
     3061 aaacagcccc ttgatccaaa ctgttcgagc atatgaggaa gcattgatcg aactagggga
     3121 aaacggagaa gaccctgatg tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa
     3181 agaagatgct tggactacag ataccaacgc aaagattatt ttgcaaaaat taggaatcag
     3241 tgaattagat aaaaaaatca gtactttatc aggtggtcag aaaaaacgag ttagtctggc
     3301 gcaagtgttg attgatgaac ccgatttact attactagac gaaccaacaa accatttaga
     3361 ttatgaagcg atcgagtggt tggaaaacta tttgaaacaa taccgtggcg cactattgat
     3421 ggttacccac gatcgttatt ttctagatag agtagcaaac cgaatcttcg aactttcatt
     3481 tggaaaatta tacgagtata aaggaaatta tgaagcttac gtcttagaaa aagcagaacg
     3541 tgatcgtgta gaagtagaac aagaagaaaa gcgcaagcgg ctttataaac aggaattagc
     3601 ttggatgagg gctggcgtac aggcacgtgg caccaagcag caagcacgga tcaaccgttt
     3661 cgaagatcta aaggagaact tgtaccaagt aaaccaagaa gatgatttag agctgaacct
     3721 agcgacacaa cgtttaggga aaaaagtact ggaaatcaaa gatggcagtt accgtataaa
     3781 tgaccaaaca ctgctagaac atttagattt attgattcaa tctcgggaaa gactaggtat
     3841 tactggtaaa aatggtgcgg gaaaatcgac attgcttaat attctagctg gcagaattcc
     3901 cctggatagc ggtacaatgt ctatcggtga aacggttcat cttgcgtatt atacacaaga
     3961 aaatgaagaa atggcaccag ataaacggat gattgcttac ttacaagaag ctgctgaaga
     4021 agcaaaaaca gcagatggca gtcaaatcgg agtagcagaa ttacttgaac gatttttatt
     4081 tccaagattt atgcatggga cactgatagg caaactttca ggcggagaaa aacgccggtt
     4141 atttttgttg aaactgctga ttcagcaacc taatgtttta ttattggatg aaccgaccaa
     4201 tgatttggac atagcaacat tgacgatttt ggaagattat tttagaagtt ttccaggtgc
     4261 tgtgatcact gtttcccatg accgttattt cttggacaag gtagcagata agttattagt
     4321 attccaagga aacggaaaac aagaacttta ttacggaaac atgtccagtt atttactgaa
     4381 gcaaaaagag acccagcagc ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc
     4441 tgctgaaaag aagaaattat cttacatgga acaaaaagaa tgggaaacga ttgaagatga
     4501 gattgcagaa cttgaagaaa aaatctcatt gctccaagaa gagatgaatc atcaaggaga
     4561 taatttcact cgattacaag aattgcaaaa cgacgtatct gaaacagaag cacaacttga
     4621 agaaaaaatg gcacggtggg aatacttgag tgaatgggta gaagattagg aggaaaaagg
     4681 atggaacaag cgtatcttga cttaggaaga aaattgttgg aagaagggca tcgtaaagaa
     4741 gacagaacag gcacaggcac attaagctta ttcggatacc aaatgcggtt tgatctgcaa
     4801 aaagggtttc ctttgttaac aacgaaacga gtaccttttg gattgatcaa aagcgaattg
     4861 ctttggtttt taaaaggaga tacgaatatc cgctatctat tacaaaataa aaaccatatt
     4921 tgggatgaat gggcatttga acgctacatc aaaagtccag actataatgg accagatatg
     4981 actgattttg gccatagatg tttgacagat cctgaattca atgaagaata taaaaagcag
     5041 agcaagcttt tttgcgagaa gatcctaacc gatgattcct ttgctgaaac atacggcgat
     5101 cttggacaca tctacggcta tcaatggcgt cactgggaaa caaaagacgg tggctttatc
     5161 gatcaaatca aacaagtgat cgaagcaatc aaaaagactc ctgattctag aagattgatc
     5221 gtttctgcat ggaatccgga agatgttcct agcatggctt tgcctccttg tcatacgatg
     5281 tttcaatttt atgttcaaga gggacggcta agctgccaat tgtatcaacg cagtggggat
     5341 gtgtttctag gcgttccgtt caacattgca agttatgcgt tgctgactca cctaattgca
     5401 catgaaacag gtttagcggt tggcgaattt gttcacacat taggagatgc tcatctttat
     5461 atcaatcata tcgaacaaat gaaagaacaa ttgagccgag aaatacgcgc atttccgact
     5521 ttagttttga atcaggaaaa gcagtcagta tttgattttg agatggaaga tatccaatta
     5581 gaaggatatg acccacatcc ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga
     5641 tgagaaaatg ttagctgcaa tttgggcaca ggatgaaaac ggattgattg gaaaagaaga
     5701 ccgtcttcct tggcgtttgc caaatgatct gaaattcttc aaacaaatga cagaagcaaa
     5761 tacactagtg atgggacgaa aaacattcga agggatgggt agccgtccgt tgccgaatcg
     5821 tcagacgatt gttttgacga gagattcttc ctataaagca gaaggcgtgc atgtcatgca
     5881 tagtgtggaa gacgtgcttg ctctggaaaa agaaacagac ggtatctttt tcatcgctgg
     5941 agggtcagct gtttatcaag aatttctgcc attttgtacg atattatacc gtacagtcat
     6001 ccatcatgta ttcgatggag atgcttattt tccacctgtt gactggtctg attggtcact
     6061 gatcaatact agtcaaggag aaatggatga aaaaaatcgt tatccgcatc aatttgaaac
     6121 gtatcagcgt aatgagaatc ctgtaaaaag cgggacaggc gaattgacga tataaaaaag
     6181 ctatggatag actagctgtt atttagagct gtcccatcca tagctttatt ttttttatta
     6241 agtaaagaat aaaacagaga agtacataaa acctgcacct aacataacaa acaaatgcca
     6301 aacaacatgc atatagcgga tagaattcaa gctataaaag aatgcgccta tcgtgtaaga
     6361 aagcccgcct gctaccatca aaataacacc agtcattcca agagagtgat aaagaggaac
     6421 agcagccacg acacagagcc accccataaa aatatagatg atcgtagata cttttgatac
     6481 tctctcccgt ttatgcaacg tcaacgcttt atatataatg ccaagaatcg caaacaacca
     6541 gattaataca aataaagtcc aacctagcca tcctcggata ctcagcaaac aaaaaggagt
     6601 ataacttcct gcaatcaaaa gaaaaatcga actatgatcg aatacttgga acaccttttt
     6661 tgcttttgta aaaatcaatg aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat
     6721 cgatccatag atagcataag agacaatatg gagggaggaa tgcaagtggg caccttttac
     6781 taaaagaacc actagtcccg cgatactcaa tccagtccca attccatggg tgactgcatt
     6841 aaacacttca tttaatattt catacgttcg attaaatttc gttttttcca tgaaaacggc
     6901 tcctttacta ggtgtaatta atgaaaaatc attatttgtc tgttatacta aaaatagaaa
     6961 ttgtcttaca tgtagtttaa catgaataag gcatcttgtt aaagaaagag agagagaaaa
     7021 taatgacaaa tgtaaaaatc gtaacagatt cttcatgtac gatggaaaaa aatgtacgcg
     7081 atgctttagg cattcgtgtc atgccgctat ccatcatgat cgacggaaca gtatatcccg
     7141 atgatgacca actagacggc gaaagattta tggaaatgat ggcgaaagca aaaaatctgc
     7201 ccaaaacaag ccaaccacca attggagaat tcatctcgat gtatgatgag ctaggagaag
     7261 acggcagtga agtgatttcg atccatatga caaaaggctt gagtggaaca gtagaagcag
     7321 caagacaagc cagtaatcta acaaaaacga aagtaacggt tatcgacagt gataccactg
     7381 accaaggatt atcttttcag gtaattcgag cagctgaatt agcaaaagaa ggtaaaacag
     7441 tagagaaaat ccttgaagaa gtcaaaaaag tacaagacaa tacaaaatta tacatcggaa
     7501 tatccacact agataacttg gtaaaaggtg gaagaatcag ccgaacaact ggtttgctgt
     7561 ctagtgtttt gaacatacgc gtggtgatga atttcgccca ttctgaatta atccctgtaa
     7621 ctaaggggcg aggaaagaaa acgttcgata aatggtttaa taatttgaaa aatgaactga
     7681 aagaattgcc aaatgtgcga caaatcggta tttcacatgc ggatgcaaaa gaattggcgc
     7741 aagaattcaa agagggtttg caagaattat tcccggacat gcatatccct gtactgcata
     7801 cgaatccggt tatcgctacg catactggta aagacgcgtt tgcaatcatg tattatgtag
     7861 attagtcgaa aagaggataa gccctcagcc tgaaaggctt gcagcttatt ctctttttct
     7921 ttataaaatt aggaaaataa ttgttttctt ttttcataaa gactaccttt ttgataaaat
     7981 ttttgataac attagaacca atcgaataca agaggtgaaa aaatgaaatt aacgaaacga
     8041 catttgatcc tcgttttgtt cccattatta attgcgatca tcatctatgc aggtttagct
     8101 gcttctatac cgaaagctgg cccactaatg aaaccagtaa ctactgtgag agaagatcca
     8161 aataaaaaag aaaaacttca ctacatagct atcggagatt cgctgactga aggagtagga
     8221 gatcaaacca agcaaggagg attcgttccc cttgtcgcaa atgatctaaa agaccgttat
     8281 aatttgaccg cggttgaaac agaaaactat ggtgtaaatg gtgaacgaag tgaccagatc
     8341 ttaaagcgag taaaaacaaa agaagaaatt cgaaacaata tcaaaacagc tgattttatc
     8401 acactgactg ttggcggtaa tgatttgatg aaagttatcc aaaataattt attcgggtta
     8461 acggtcaatt cctttaaaaa gccactgaaa aactatcagg aaaatgtcac agaactattg
     8521 gaagaatttc gatccctcaa taaaaccgca cctatttaca ttttaggcat ctacaatcca
     8581 ttttatttga actttcctga gatcactgac atgcagacaa ttgttaacaa ttggaacaaa
     8641 ggaacagaaa cggtgacaaa aaaaatgccg aactgctact ttatcccaat caatgatttg
     8701 ctttatcaag gattgaatca ggaagtaggt gtcacagaag aaggaacaga tcaaacaact
     8761 agcacgacag aaaatctaaa tggcatcaaa aataatgtgc tctatgaaga agatcatttc
     8821 catccaaata atttaggtta tcaattgatg gcaaatgcag tgagagatca gctgatcgag
     8881 acacaaaacc aatggttaga gaaggaggat gatcaataaa catgaaaaca agatctgacg
     8941 tatcttcaaa caaaacaaag aaaacaaatt actggaaatt cgccttcctt atccttcttg
     9001 gggtagtact tggttcgacc gtatttttag gcagccgtat ttttgctaat cgggaaccag
     9061 aactcccaga aataccagct cttacggaaa gacaaggaga tcctgtctta acgatcaatt
     9121 caaataaaga aaaagtcaat caaatcatca gctttttcct tagtgagtat caaaaagata
     9181 gtgatattga atataaattt tatttggaaa atgaagcact actcaatggt actttcgaag
     9241 tactaggttt tcctatcgat ttctatttat attttgatcc gtatgtgatg gaaaacggaa
     9301 atgtccaact aaaagctaga agtctttcta tcggaacatt gagtttgcca atcaaagatg
     9361 tgatgaatat gatcaagaga aactataaat taccagattg gatcgaagtc aatacagatg
     9421 atctgactat catgcttagg ttggatcaat tccgaatgca aaacgggatg tatattaaag
     9481 cagataaaat cgacttagta aatgatgaca ttcggtttag tctatatttg ccaaaagata
     9541 cctctgatgc ttcaaacaac tcaaatacca acaaaactag caccagcagt actgaaagca
     9601 agacaaaaga aaataaaaaa tcatcggatg agtagtatta gagggggaat cataatggaa
     9661 cgagcaatat ttgcaggagg ctgcttctgg tgtatgatcc agcctttcga cacgcttcct
     9721 ggtatccata cagtgatgtc aggatatact ggagggcatg taccagatcc gacttatgaa
     9781 caagtaaaaa gccaaacgac tggacataca gaagcagtgg agattatcta tgatccaaca
     9841 ttgatttcat atgaacaatt agtagaactt tactggcagc aaactgatcc aactgacgct
     9901 ttcggacaat tcgaggatcg cggtgacaat tatcgcccgg tgatctttta tttgacagaa
     9961 gaacagcgga tcattgctga aaaaagtaag aaacagttag ctgaaagtgg acgtttcgaa
    10021 cagccaatcg ttacaaaaat cgaaccagcc caaccatttt atctagcaga agaagaccat
    10081 caagactatt ataaaaagca tcctgaaaat tttaaaagaa accatgcacg ccgggctgcc
    10141 tttatcgaag aaaattggag gaacgaaggg tgaatcgaag cttttttcat tatttaatga
    10201 cactgcgagg cggtggcaca catgatgctt tatctgtttt cgctacagaa gccggtaaag
    10261 acatccaatt tcctaagcac agctctagct acgaagaaat ttctaattat ctggaactga
    10321 atgtcgacta tttgccgtcc atggatatct ttgacgaagc atgggaaaaa tatttagaaa
    10381 ataatcatga catataagag atagttttgt actttatgat caaaaaagtt ttttgtccaa
    10441 tagcctaatt gaaagaatta tttttatttc agctatctag tcgcattttt ttgatatttt
    10501 tcttataata aagaaagaat gaaaaaaaga gggatggatt gatagagatt gacaaaaaaa
    10561 caacgctata ttaatcgctt ctggcttgta ttcaaatact tacttgtttc tgtattcctt
    10621 gcgttcatgt tgcggggatt tttattcatc ccggtaccag tggaaggcaa ttcaatggaa
    10681 aacgtgttga agcaaggtga tatggttgtg atggaaaaat tttcggagat cagacgattt
    10741 gatattgttg tttttcaatt agcagatggc acgatttata tcaaacgagt aatcggtctt
    10801 ccaggagaaa atgtcagcta ccaaaatgat caattgaaga tcaatgggaa agtagttaag
    10861 gaaccttatc taactaaaaa cctgaaatca gatcatgcaa atgcatccta tacgacggac
    10921 tttacactac aagagctaac gggacaatcc aagcttccgg aagataatta ttttgtactt
    10981 ggagataatc gaagagtttc aaaagatagt cgttcgtttg gcacgatcaa taaaactgat
    11041 attctaggta aagcaagatt tgtctattat ccacttgatg aaataaaatg gatccagtga
    11101 gaaacaataa agcgtgcagt ctgttgagac tgcacgcttt attgtttaat ccaaaatcag
    11161 tttactacct tagtacgtaa aatcaaacag ctcgtttaaa cacttttcta gcctctaaag
    11221 ttttgataaa tgaataaaca accattattt gaatagggat ttggatcata ttcctgatga
    11281 atctacctag aaaaattact ttgaaatctg caccaaacaa aacagacatc agccaagaat
    11341 tgataaaaag gctgactggg atcataatca ataattgggc agcgatgacg gaaggcaggt
    11401 ttatcttttt atgatataaa aataagccat taataaatcc accaaggaaa gccgtgataa
    11461 gaaaagcagc ttgaaacgtt cctgaaccaa atatcgtgaa atttaaagtg ttcgacaaca
    11521 tttcaagcaa accagcccag atcggtccat atagataacc cataagcgcc atacttaaaa
    11581 atccgaatgt gatttgaaaa taagcaggac caatcgctat cttgtataag atgacatcaa
    11641 gggctaataa aagagcagag gtcgacaaca atcttgcatt catttcgggc aatctaaaaa
    11701 cgtcttttgc aatcaaagtg ttcaaaatat tctctccttt gtcagttaat aataaaagga
    11761 taccataaaa tcaccaatgt gtcatgacac attggtgatt tacatacact ggcatctaac
    11821 aattaaaaaa agggtgagaa gactgctttt ataaactgta aaaaagagat tgtacgaata
    11881 aaaaaaatag aggcggagaa tacttatcgt atcatccgcc tttgtttttt tgttataaaa
    11941 aacgtcagat tagttatctg cttacatctt catccatacg ggttctaatg tatttccgtc
    12001 tagatcttgg acttctaatg aaaacatttc ttcttcaggc attcccatat ctacttcgtg
    12061 gaaagtacca ccattttctt ttgcaatttg agcaaatttt tttacttcat ccgcattttc
    12121 aacactaaac gaaatcaaag caccacttgt ttttttatta tcagctattt ctttatcttt
    12181 tataaatttt ttataaaaat catgagagag aagcattatc caaaaagtgt catcccatac
    12241 cattgaacca gcttcttcag tagaaaattc ttcatttttt ttaaaaccca gcttttcata
    12301 aaaagcaacg gaacgattga catcagcaac aggaaaatta acaaaaacca tactattcat
    12361 ttgtttcgtc tcctttctgt tttgttcgtc tatcatctct tatttaggaa acgctgtcaa
    12421 aaatttagat tcatatatag aaaccaaaat aaaaatttgc atcatcaaaa aagtgaagat
    12481 gtgatagaat gaggactggt tcactttcac ttaactaata gatggaattt ctttataata
    12541 aaaaagtgaa tttatattga aaaagaaaaa aacaagtaaa gccggggaat attccggtat
    12601 gaaaggaatg gagagaacaa tgaccatcca atggtttccg ggacatatgg caaaagcccg
    12661 acgagaagtt tctgaaaaaa tgaaatatgt ggacatcgta tttgaattgg tagacgcacg
    12721 attaccttta tcttctcgta atccaatgct agatcagatc attcagcaaa aacctcgcct
    12781 tgttttattg aataaagcgg atctggcaga tccacaacag acaaagctgt ggcaagaata
    12841 ttttcgtaaa caaggccatc ttgcattagc tatcacggca caagaaagta aaggaattaa
    12901 aggtctagta cctgcggcta aagaagcatt gaaagaaaaa cgggcaagag atgctgctcg
    12961 ggggatcaag cctcgcgcta ttcgggcaat ggtcttaggt attccaaatg ttgggaaatc
    13021 tacgttgatg aatcgactag taggcaagaa aattgcacag acaggaaata agcctggcgt
    13081 aacaaaaggt cagcagtggc ttcgtttagg taatgagttg gaattgttag acacaccagg
    13141 gattttatgg cccaaatttg aagatgaaga aatcggcaaa aaactagcgc tgacaggcgc
    13201 gatcaaagac cagcttgtcc atttagatga tttagcgatt tacggattag acttttttgc
    13261 ccgatattat tcaggaagaa tcagcgatcg ttatggttta tcaatcgagc aagaagaaaa
    13321 attggcaccg gaattactga tggatattac agcaaaacgt gggtataaag aagactatga
    13381 tcgtggcagc gaattagtcg tgcatgatat tcgtcaagga aaacttggac gatacacact
    13441 ggatcgttgc gaagaaatgg agcaaaaaag tgatgactga atccatccaa agtatcaaag
    13501 aacgattgaa aagcatacag agaatagatg atcctacact agaatcatta agaaatgatc
    13561 cacgcaaagg cgtgcagcaa gcactggctt cttttgaacg acgattgaaa aaggaagcat
    13621 taattaagga aaaatatgaa gaaatgtttt gctttgaaaa gcaagggtac gcacaaggtc
    13681 accaatggat tgccggcatt gatgaagtag gaagaggacc attagctgga cctgtcgtgg
    13741 cagctgcagt tatcttgccg aaaaatgaga tcatattagg gctgaatgac tccaaacaat
    13801 tatcagagaa aaagcgagaa cttctttatc aacaagttca agaaaaagcc ctggcaatcg
    13861 ggatcgggat cgttgatcag cagacaatcg atgatgtgaa tatttatgaa gcaagtaaat
    13921 tagcgatgat ccaagcagta gaaaaattgt ccattgtacc aagctatttg ttgatcgatg
    13981 cgatgacctt ggatttgcct attcagcagg aaaaaataat caaaggggat gcacgatctg
    14041 tgtcgattgc tgctgcaagt atcattgcta aagtatatcg agaccaattg atgaaagaat
    14101 atgacgtgct ttatcctgga tatggtttct cgcataatgc aggttatgga acaaaggaac
    14161 acttagaagg acttgaacga caaggaatta cgccaatcca tcgtaaaagt tttgcacctg
    14221 taaaaaaata tttacaataa aaaacagccg cttttacgta tcttattaga aagagagacg
    14281 aaaggcggtt ttatgatgta tcaaatagaa gaaaatttat tgaaacttgc ttatacaaaa
    14341 ggaatcagta gtcaaggaaa atgggcactt gctaactgga tgatgcagta tccttataaa
    14401 gaaatcggat ttgaagacat cattaagata ggtaaaataa ccaaacaccg ggagctgttt
    14461 cgcaaaagtt ggcaggaaat tcacgctaac tggggcaaaa tccaatcgaa acagtcgttt
    14521 atcacttgct ttgatcccaa atatccccct cagctgcttc acttgaccta tccgccaatt
    14581 gtactgtttt ataatggtga tctatcttta ctttcttgta acatgttaag cgttgttggt
    14641 ggaagacaca cttctgcttt agccaaaaaa acagtttatt atttactaaa accagtgata
    14701 gaagctggat atgtgatcgt cagtggctgc gcgaaaggga tagatacata cggtcaccaa
    14761 gcagcaatct ctcatggtgg acggacaatc gcagtcattg gaacagggat agagcaagtc
    14821 tatcctgctg aaaatcgtga cttgcaagag gtgattgcaa atgatcatct tcttttgagt
    14881 gaatatcctc ctcaatccgg cccgaaaaag cattgttttc caatgagaaa tcggattatc
    14941 gcagctttgt ctaaaggaac atgtgtcttt gaagccaaga aaaaaagcgg ctctctgata
    15001 acagcacagc aagcattgga tcttggaaga acagtttttt ctgttccagg aaatatttta
    15061 acagaacatt ctaccggatg tcatcatttg atccaagacg gtgcagtttg cacggtttcg
    15121 ggtgaagata tcttggcaga atttaaagaa taacaccata cttttcattt gcttttcttg
    15181 acaaacgttt tctgaatgga tatgattagc tacgatttgt atgaatcctc ttttaatagt
    15241 aggaacacga caatggaaag gagtccattc agtaatggcg tataaatatt tagtgattgt
    15301 agaatcacct gcaaaagcca agacaattga gaaatatctt ggacgaaact ataaagtaat
    15361 ggccagtgtt ggccatataa gagatttacc gaaaagtaaa atgggtatcg attttgaaaa
    15421 caattatgaa ccccattata tttctatacg cggaaaaggc gatgtcatca aaagcctgaa
    15481 agccgcagca aaaaaagctc aaaaagttta cttggcaagt gacccggata gagaaggaga
    15541 agcgattgct tggcatttag cgtacctact tgggttggat ctgaaagaaa aaaatcgggt
    15601 ggtcttcaat gaaatcacaa aagacgcagt caaagcagct tttaaggaac caagaacgat
    15661 cgatgtagat ttagtagatg cacagcaagc tcgtcgtacc ttagacagaa tcgttggtta
    15721 ttcgatcagt cctattctct ggcgtaaggt caagaaaggg ttaagtgcag gacgtgtcca
    15781 atctgtcgct ttaaaaatta ttattgaccg tgaaaaagag atccgagaat ttgttccaga
    15841 agaatattgg agcatcgacg gtaattttaa aaaagctcgc aagaaattca aagcaaattt
    15901 ctggggaatc gacggtaaga aaaagaaatt accagatgca caaagtgtaa aagaagtcac
    15961 tgctagaatt gatggaacag acttcgaagt taaaaaagta gaaaagaaag agcgcaaaag
    16021 aaaccctgcg aatcctttca ctacaagtag cctgcaacaa gaagctgccc gtaagttaaa
    16081 ctttcgaaca agaaaaacca tgatggtagc acagcaatta tatgaaggaa tcactttggg
    16141 taaacaagga acagtcggct tgattacata catgcgtact gattctaccc ggattgccga
    16201 tacagcaaaa gcagaggccg cagcatttat tgaagatacg tatggaaaag aattttctag
    16261 acatgatcac cgaaagatca aaaatgctca aggcgcacaa gatgcccatg aagctgtgcg
    16321 tccaacgagt gtgcttcgta caccagatga actcaaacaa tatttagata aagatcaatt
    16381 gaaactttat acattgatct ggtcacgttt tgtggctagt cagatgacac cagctatttt
    16441 agatacaatg aaagtgacat tagaacaaaa tggtgtcatc ttcattgcga acggttcaaa
    16501 aatcaaattc aaaggattca tgcaagtgta tgtggaaggt agagatgacg gcaaagaaga
    16561 gaaagaaaat attcttccag aacttgaaga aggcgatgta gtccaatctg ttgatattga
    16621 accaaaacaa cattttactc aaccaccagc acgttttagt gaggctacgt tgattcgtgc
    16681 actagaagaa aacggggtag ggcgaccttc cacttatgca ccaacacttg atacgatcca
    16741 aagacgctac tatgtaaaat tgacacaaaa aagatttgag ccgactgaac taggagaaat
    16801 tgtcaattca ttaatctgtg aattcttccc tcaaatcgtc gatatccatt tcacagcaga
    16861 aatggaagga gatttggata aaattgaaga aggcacggaa gcgtgggtca aagtggttga
    16921 tcgtttctat aaaccgtttg aaaaagaact gacgaatgcg gaagaaaaaa tcgaaaaaat
    16981 tcaaatcaaa gatgaaccag caggattcga ttgtgatgtc tgtggacatc cgatggtgat
    17041 aaaattaggg aaatatggga agttttatgc ttgcagtaat tttcctgatt gtcggaatac
    17101 gaaaccaatc gtaaaagaaa tcggtgtgac ttgcccagta tgtcatgaag gacaagtcat
    17161 tgaacgaaaa tctaagaaaa acagactatt ctatggttgt tcccgtttcc cagaatgtga
    17221 attcacttct tgggataaac cagtgggcag aaattgtcca aaatgtgacg gatatctggt
    17281 cgagaagaaa gtcaaaggcg gtaaacaagt agtctgtgta aatggagatt acgaagaaga
    17341 catccaaaaa taatagaaaa gaggttcttt cattgaaaat ggtaaatgtt gttggagcag
    17401 ggcttgccgg aagtgaagct gcttggcaga tcgcacaagc aggagtccct gtccgtttat
    17461 atgaaatgcg tccagtcaaa tcgactgagg cgcatcacac aagtaatttt gctgaactag
    17521 tatgttccaa ttcattaaga ggaaacagtt tggctaatgc agtagggtta ttgaaagaag
    17581 aaatgcgccg attgaattcg gtagtaatca cttcggcaga tgaaacagca gtgccagctg
    17641 gtggtgcctt agctgttgac cgcgattctt tttctgaaac aattacccga aaagtcaaag
    17701 agcatccatt agtaacagtg ataaacgaag aaattactga aataccggaa ggaatcaccg
    17761 tagtagcaac aggacctttg acttctcatc cattagcaga aagcatcaaa gcattcaatg
    17821 gttcagatgg tttttatttc tatgatgcag ctgcaccaat cgtagacaaa gctacgatcg
    17881 atatggataa agtatatttg aagtcccgct atgacaaagg agaagcagct tacttgaact
    17941 gtccgatgac agaagaggaa ttcaaagctt tccatgaggc actaatcaat gccgaagtgg
    18001 cagaacttaa atcttttgag aaagaaaaat attttgaagg atgtatgcca attgaagtaa
    18061 tggcacagcg aggattcaaa acgatgcttt tcggacctat gaaaccagtt ggattggaag
    18121 atcctaaaac tggcaaacgt ccttatgctg ttatccagct gcgtcaagac aatgctgcag
    18181 cttctttata caacatcgtg ggctttcaga ctcacttgaa atggggcgag caaaaacgtg
    18241 ttttccggat gattcctggg ttagaaaatg ctgaattcgt tcgctatggt gtgatgcacc
    18301 gaaacagctt tatgaattcg ccagaattgc tgaaaccgac ttaccaatca acgaaacgcg
    18361 aagatctgtt ctttgcaggt cagatgactg gtgtagaagg ctatgttgaa agtgcagcga
    18421 gcggactact tgctggaatc aacgccgcac gcttagcaaa agaaatggaa ccagtcgagt
    18481 ttccacaaga aacagctatt ggaagtatgg cttactacat tactcatgcg gaaggaaaac
    18541 atttccaacc gatgaatgcc aactatggtc tgtttcctga actgccagag cgcattcgag
    18601 acaaaaaagc aagatatgaa gcaattgcac aacgagcttt aaaagctaat gaggcaataa
    18661 aaacagaatt agaaaaaact gcaatcccaa atgagtaagg aaactccatg aagagggtga
    18721 gacggatagt ctcgctcttt ttcttgcgtt tttcttaaga atttctcaaa caaaatgtga
    18781 ggaattgcgc caattgtgaa caaacttaga aatattggtt aaattctgac aatttatttg
    18841 tctttttata aacgctgtgc taaattattc tctgggacaa aaggaaggtg agaaaatgac
    18901 agaagccacg tgggaacaaa aatttttgcg ttatttgatt gtcgaaagag gatattcaga
    18961 aaaaacgaaa gaagcatatg aagaagacat tctccatttc aaacattttt tggaggaatc
    19021 tggagacgct gatctgctga aggcggaata ttttacggtt cgtgtctatt tgagtgcatt
    19081 agctgatcat cgtcctagct atagcagaaa ttctatcagt cgcaagatag ccagcctacg
    19141 ttcgttttat cagtatcttc tcaaagaaga agtgataaaa gaaaatccat tttcttacgt
    19201 tcatctcaaa aagaaaaatt tgagactgcc acgttttttt tatgaaaatg aaatgcaagc
    19261 actttttgac agcgtagcag gagatagtcc tttggatttg aggaaccgcg ctttactgga
    19321 agtcctttac gggtcaggca tccgtttaag tgagtgtagc agtttgtcgg tatcagacat
    19381 tgattttgat tcagaggtca tgttgatcca tggtaaaggg aacaaagaga gatatgctcc
    19441 tctaggttct tttgctcaag atgctctgca agaatatttc agggaaggcc gccaggtatt
    19501 gatgacaaaa tatcacgaag agcatgatat cgtttttgtg aatcatcatg gaaaaccaat
    19561 cacgcctact ggaattgaat atgtcttgaa tcaaatgatc aaaaaaagca gtttggatag
    19621 tgatatccat cctcacatgc tgcgccatac atttgctact catttattaa ataatggggc
    19681 agatttgcgg acagtccaag aattgctagg acatgccaat ttatcgacta cccagatcta
    19741 tgcccatgta acaaaagaaa gtttacaaaa aaactatcgt tctttccatc caagagcgta
    19801 aaaataagga aaagaacaaa tttaggagga aacaaacatg gtcgaatcac catttcattc
    19861 aaccactatc tgtgccgtcg aaaaagacgg gaaatttgct atggctggag atggccaagt
    19921 aacaatgggt gaatctgttg tcatgaaagg cactgcaaag aaagtacgac gtatctacaa
    19981 tggagaagtt gttgtaggtt tcgccggaag tgttgcagat gcgtttactt tagaagaaaa
    20041 atttgaaggc aaactaaacg aatataacgg gaatctacaa cgcgcagctg tcgaattagc
    20101 tcaagaatgg cggacacagc aatctatgca aaaacttgaa gcgatgctga tcgtaatgaa
    20161 tgataaagaa atgcttcttg tttcaggtac aggagaagtt atcacaccag atgatggtat
    20221 cttagctatc ggatcaggag ggaattttgc gttggctgca gcccgagcaa tgaaaaacta
    20281 tgctcaaaac gacatgagtg cgaaagaaat tgcagaaaat gcgttaaata ttgccgcaga
    20341 tatctgtatc tttactaatc ataatattat cgtagaagaa ctgtaagagg tgagtagata
    20401 tgtatgaatt aaaacaaaca ccaagacaaa tcgtaagtga attagatgaa tatatcgtag
    20461 gacagcacgc agcaaaaaaa tctgttgccg ttgctttgcg taaccgctat cgccgtttgc
    20521 agttagatga aaaaatgcaa caagatatta caccaaaaaa tatgctgatg atcggaccta
    20581 caggggtcgg taaaacagaa atcgcacgac gtttggcaaa aatcgtcaat gcaccatttg
    20641 tcaaagtcga agcaacaaaa tttactgaag tcggttatgt cggaagagat gtagaatcaa
    20701 tggttcggga tttagtagaa aatgcgatcc aaatcgtaga aaaagagcag tacagccgtg
    20761 tatacacaag tgctaaaaaa aatgcagaaa aacgtttggt aaaaatcttg gtacctggta
    20821 tcaaaaaaga acagaaacaa tccactaatc aatttgaaca aatgatgaat ctttttaatg
    20881 ctagtcagca aaataccgct gaaccgcaag aagaagtgac agaagatatt cgaatcaatc
    20941 gccgtacagt cttggaacaa ctacaaaagg gtttactgga tagtcgtgaa gtaaccattg
    21001 aagttgaaga ccaaaagaaa cctgcaccaa tgatgaacaa tggattggag caaatgggga
    21061 tcgacctaaa tgaaactcta ggtgctttga caccgaagaa aaaagtggaa cggacggtaa
    21121 cagtcaaaga agctttagag ttattgatca aggaagaatc agcaaaatta gtcaaggatg
    21181 ctgatatcca tagtgaggca atcaaattag cggaagaaag tggaattatt tttatcgatg
    21241 aaatcgataa gatcacttct aaatcacagc aatctggaga agtatccaga gaaggcgtcc
    21301 aaagagacat attgccaatt gtcgaaggtt cacaagtaaa cacaaaatac gggatcatcc
    21361 aaacagatca cgtcctattc attgcttcag gagctttcca tttgtctaaa ccaagtgatc
    21421 taattccaga acttcaagga cgcttcccga tacgggtaga attagatgat ttgacagctg
    21481 aagattttgt gaagattctg accgaaccaa ataatgcttt agtaaaacaa tacgtcgcgt
    21541 tgatcggtac agaaaatgtg tcagttacgt ttacaaaaga agcaattgaa cggatcgctc
    21601 aaatcgcttt cgatgtcaac cgtgacacag ataatatcgg cgcacgcaga ctgcatacca
    21661 ttttggaaaa actactagaa gatctattat ttgaagcacc agatatgcaa atgggagaaa
    21721 taacgatcac agaagcatat gtggatgaca aacttggctc aattgcggca aatgaagatt
    21781 taagtcgcta tattttataa aaaatcggag gacgacaatg acagacttgc taacgaaaac
    21841 ccgcaagata aacacacttt tgcagcaaaa gaatactttt gacatgcaag cagatttgcc
    21901 ttacgataaa atggctgtca cactaggtgg tattcttgac agcaacacct atatcatagg
    21961 aagtgacgga gtattactag gttataatga acgtcacgat gtgaacaatg aacgaatcaa
    22021 aacaatgttt gttgagaaac aatttcctga atcttatact gatacagtgg cacagttgat
    22081 gaaaactgaa gctaatatcc caattactag tgatattact gcatttccgg tagagtcaag
    22141 agagaaatat ccatttggtc tgacgactgt cgttccgatc tttggagctg gcgaacgttt
    22201 gggtacgatt atcttatctc gtatggaaca atcgtttgca gatgacgatc tagtcttagc
    22261 agaatatgga gctacggttg tcggtatgca gatcctttat cagaagtctc gaagcatcga
    22321 agcagatgta cggagtgcaa cggctgtcca aatggcaatc aacacattat cttatagcga
    22381 attgaaggct gtacaagcta tctttgaagc attggatgga gatgaaggtc gtctgactgc
    22441 ttctaatatt gccgatagta tcggcatcac tcgttctgtg atcgtcaatg ctttacgaaa
    22501 attggaatca gctggaatca ttgaatctcg ttcgttaggc atgaagggaa cttatttaaa
    22561 agtattgaat tcccgtttta aagacgagtt agcaaaacac agttattaga agtaagggga
    22621 ctggggcaga tagaaatgtt tgttccagcc ttcccatttt aaaaagtaag tcgaagttgg
    22681 aggaatggca atgactgtta ttattcaaaa tgaccagctg atcgccgaaa tcgctgaaca
    22741 cggtgcggaa ttgatcagtc tgaaatcaaa agaacatggt ttagagtata tctggcaagg
    22801 tgatccagcg tattggggca gacatgcacc ggttttattc ccttttgttg gacgtttaaa
    22861 agatgatcag tatgtttatc aagggaaaac atatcaaatg gggcaacacg gatttgctcg
    22921 ggatatggat tttgaagtca ttgaacatgg acaagaaatg gctagtttca tcttaaggag
    22981 tacgccggag acattagaaa agtatccatt tgattttgaa ttagtgatca gctatgaatt
    23041 aggaggagac ggcatcacgg ttcattatca agtggcaaat accggtgaaa aagaaatgtt
    23101 cttttctatc ggtggacatc ctgctttcaa tgtacctctt gaagaaggat tgtcatttga
    23161 agatttctat ctggcatttt ctccattgaa atcaagatta cgcctaccat tgaaaggccc
    23221 attcatcgat atggatcaaa agacgttagg tcagacaaat acgaatctgg ctttgaatcg
    23281 tgaacttttt gagcaagatg ccttggtttt tgaaacaagg ggtctaaatg cgtacgccat
    23341 tcgaagtgaa aaatcttccc atagcgtaac tttaagctat aaagatatgc cttatgtagg
    23401 gatctggtct ccttatccaa aagaagcgcc tttcgtttgt atcgaaccgt ggtgcggaat
    23461 cgctgacacg gtgaacagta gtggaaactt gatggaaaaa gtagggatca atcaactagg
    23521 tgcccatgaa atcttcaaga caaaatattc catcacagtg aagtaagagg ttgtgaccga
    23581 gaagttagca gggaacaccg tttaccaagg tgtgtaaaaa agctatcctg taagtaataa
    23641 gggagtgtga catcgatttg tcacactccc gttttatctc tttggaatga aaaagtatca
    23701 ggattttttc ttgctgctat tcaaaccaaa cgggattcga ctttcagtat ggtttctgat
    23761 acgcttgata ttgtctttat gccgataaaa gataaaggaa gaaactaaaa aggcaatgat
    23821 cgtcagtaac caattgggct tagccaaaat cacaggagct acataaggaa ggacgatcgt
    23881 agataaagtg atcaatactg cactgatcat actgctcaaa ctcaccatac ttgtacaaaa
    23941 taaactaacg ataaagatga tacaagaata aacaaaaaat acaggattaa atcctaaaag
    24001 cattcctgca cttgtcgcaa cagcttttcc tcctttgaac ccagcaaaaa tcggaaatgt
    24061 atggccaaga atggcgcata ccccaaacca aagagggtta atcccagaaa ggtggaagat
    24121 catcggcaaa caagtagcca atgtaccttt taaaatatcc ataagcagga caacaatccc
    24181 ggctgttttg cctaatacac gaaatgtatt ggtcgttcca gtattaccgc tcccgtgttg
    24241 tcggatatct tttttgaaaa acaatttgcc aatccagaca ccggaaggaa tagaacctaa
    24301 aagatatgct aaaataagta ataagatcaa tttcattttt taccctcttt caagttgaaa
    24361 actatccttt attttagcat gaataggtaa caggaacaat tgaaaaaatg aaagatacaa
    24421 gtataataga tggaataaaa agaaaagcaa ggggtggcag aaatgtctgt aaaaaatcca
    24481 acacaagaaa cgattttcaa ctatttggaa gaagcaaaaa atattgccgt cgtaggcttg
    24541 agcaataaac cagaacgaac aagttacatg gttgcacagg tattgcaaga caatggatat
    24601 aaaatcatac cagtaaatcc attattggct ggtgaagaaa ttctaggcga gaaagtctat
    24661 gcgaatttga cagaaatcga tgatccaatc gatatcgtag atgtatttag acggagtgaa
    24721 tttttacctg aaatagcagg tgattttttg aaaactgatg ctaaagtgtt ctgggcacaa
    24781 ttagggattg aaaacaaaga agcagctgag atgctaagaa aagcaggacg tacagatatc
    24841 gtaatggatc gttgtattaa gattgaactt ggtaagttga aagaaaacca aaaataaaaa
    24901 tgaccacaaa gaagagatga tcgatagagg ggaatgtcga aaaagcggtt aggagataat
    24961 taaaattgat ttctcgtcta ccctcttttt tttcacgacc ttttctagta tgataatcgt
    25021 ggtgcggttt tgccgcaaag tattattgaa ggagcgtttg ccttggcaaa aaaagtaaac
    25081 aacgaataca atgactcgtc catccaagta ttggaaggat tagaggcagt tagaaaaaga
    25141 ccagggatgt acattggatc cactgacagc cgaggcttgc atcatttagt ctatgaaatc
    25201 gtcgataatg cagttgacga agcattatca ggttatggca gtgagattga tgtgaccatc
    25261 catgaagaca atagtataac tgttgcagat agcggacgtg ggatgcctgt aggaatgcat
    25321 gcttccggaa taccaacagt tgaagtgatt tttactgtgc tgcatgctgg cggaaagttt
    25381 ggtcaaggag ggtacaaaac ttctggtgga ctgcacggtg taggagccag tgtggtaaat
    25441 gccttatcga aatggctaat tgtaacgatc gttagagatg gcgtagagta tcagcagaaa
    25501 tttaaaaacg gcggtaaacc tgacggtact ttgaaaaaga taggaaaaac aaaaaaagca
    25561 aacgggacga ctgtccattt tttaccagat gatacaatat tcagtacaac gaaattttca
    25621 tatgaaattt tagccgaacg gttaagagaa tctgcctttc ttctaaaagg cgttaaaatc
    25681 tctctttctg atcttcgtgg agaagaacca gtcaaggaaa tttttcatta cgaagaagga
    25741 atcaaagaat ttgttgatta tctgaatgaa gaaaaagata cattgacacc agttgtttat
    25801 ttttcaggag aaaaagaagg tatcgaagta gaggtcgctt atcaatacaa tgacggttat
    25861 tcagaaaatg ttttatcttt tgtcaataac gttcggacaa aagatggggg aacccatgaa
    25921 gctggtatga aagcggcgat gacgaaatct tataatgaat atgcgagaaa agttggtttg
    25981 ctgaaagaac gggacaagaa tctggaaggt agtgatttca gagaagggtt agctgctgta
    26041 ctttctatcc gtgtacctga aaatcttctg caatttgaag ggcaaacaaa agaaaaactt
    26101 ggtacgccag ttgctcgtac cgtagtagat aacgtcatca gtgaacaaat gggcttttat
    26161 ttgcaggaaa atagcgaaat gagccagatg ttggtccgta aagcaatcaa agccagagaa
    26221 gcgagagaag cagcacgtaa agccagagaa gaaagccgaa atgggaagaa gcgtaaaaaa
    26281 ggcgaatctc ttctttcagg aaaattgaca cccgcacaat cccgaaaccc taagaaaaat
    26341 gaattgtatt tagtcgaagg ggattctgct ggcggatctg ctaaacaagg aagagacaga
    26401 aagttccaag caattctacc tttacgagga aaagtcatca ataccgaaaa agctaaaatg
    26461 caagatatct tgaaaaatga agaaatcaat acgatgatct atacgattgg tgcaggagtc
    26521 ggtcctgagt tttctatcga agactgtaat tacgacaaag taatcatcat gaccgatgcg
    26581 gatacagacg gcgcccatat tcaagtatta ctgcttactt tcttttatcg ttatatgaaa
    26641 ccgctgattg aagctgggaa agtatatatc gcgttaccgc ctttatataa agtatccaaa
    26701 ggacaaggaa agaagcaagt cattgaatat gcgtggacag atgacgaatt ggcagcaatg
    26761 attaaaaaag tcggcaaagg atatatgctg cagcgatata aaggacttgg agagatgaat
    26821 gcggaacaat tgtgggaaac aacgatggat ccgacttccc gtacgttgat ccgcgtccgt
    26881 atcgatgatg ctgcgcaagc tgaacgtcga gtaaccacac tgatgggtga caaagtagaa
    26941 cctcgaagaa aatggatcga aaatcacgtg caatttacgc tcgaagaaga tggcagtatc
    27001 ttagataaaa aagaggatac tgaaatctct ccttcagttt ctaatgattt attagatgaa
    27061 gaacgagcag ataaaaatga aaacaatcag ttatttgaag tagaatagga ggtcacctga
    27121 tggaaaatcg acaagaagta caagaattaa ctttagaaga agtgatgggt gaccgcttcg
    27181 gacgttattc caaatatatt atccaagaac gtgcactgcc tgatatccgt gatggattga
    27241 agccagtgca acgccggata ttatttgcca tgaacaaaga tggcaatacg tatgacaaag
    27301 gattccgtaa atcagcaaaa tctgtcggaa atatcatggg gaattatcac cctcatggtg
    27361 accgcagtat ttatgaagcg atggtccgca tgagccaaga ctggaagttg cgggaagtac
    27421 tgatcgaaat gcacgggaat aacgggagta tggacggtga tccacctgct gcgatgcgtt
    27481 atactgaagc cagattatcc aagttaagtg gagagatgct agcagatatc gagaaagaaa
    27541 cagtcgatct cgtgtggaat tttgatgata cagaaaaaga accaacagtc cttccagctc
    27601 gatacccaaa ccttctcgtc aatggatcga caggtatatc tgctggatat gcaacagaaa
    27661 tcccgactca taacttagct gaagtgatcg atggcacaat ctatatgatc gatcatccac
    27721 aagcgagcct tgaaaaattg atggaatata ttcctggacc tgactttcca acaggaggga
    27781 tcttgcaagg aaaagatgaa atcaaaaaag cttatgaaac aggacgagga aaagttattc
    27841 ttcgttcgaa aacaaagata gaggcaatga agggtaataa acagcaaatc gtgatcacag
    27901 aaatcccata tgaagtaaat aaagcgacct tggtgaaaaa aatggatgag atccgtttga
    27961 ataagaaaat cgatggaata gctgaagtac gagatgaaag tgatagaaca ggtctgcaaa
    28021 ttgtcgtcga attgaaaaaa gacgccaatg ctcaaggaat tttgaattat ttgttcaaaa
    28081 acactgagct acagatcaat tacaatttca atatggtcgc tatcgaccat atgactccgc
    28141 atcaagttgg actgaaagac attttaagaa gttatatcga gcatcgaaag caagtcataa
    28201 caaaacgtag tcaattcgat ttagccaaag ctcaaaaaag acagcatatc gtagaaggat
    28261 tgatgaaagc tttatcgatt ctggatgaag taatcgcaac gattcgtgaa agtaaagaca
    28321 aaaaagacgc caaaaagaat ttagtcgacg tctttcaatt tacagaagaa caagcagaag
    28381 caattgtcac attgcaatta taccgtttga cgaacacgga tatcacagag cttcaaaagg
    28441 aatccgaaag cttgattgcg caaatcacag agttgaataa aatcctttca aatgacaaag
    28501 agcttttctc tgttatgaaa aaagaattgc gagaagtgaa aaaaaattat tcttccgctc
    28561 gtctaacgac aatcgaagac gaaatcgaag aaatcaaaat tgatacgcaa gtcttagtgg
    28621 cacaagaaga cgtaattgtc agtgtgacgc gagaaggcta tgtgaagcga acaagccttc
    28681 gttcttatag tgcttccaaa ccagaagaaa tcgggatgcg agaaggcgac tacttgctat
    28741 actcaggtga attgagtaca ttggaccatg ttcttctgat cacaaataaa gcaaatgtga
    28801 tctaccgccc tgtccatgaa ctacccgatt tgaaatggaa agatgctggt gagcacattt
    28861 ctcaaacaat ctccaatctg tctgtagacg aatcgatttt agctgttttc ccatatcaaa
    28921 aaattcaagc agagaaaaca tttgtattca tcagcaagaa tggattgatc aagcaaacac
    28981 gaatgacaga gttcgaacca tggcgcacct ataagagccg tccgctcagc tgtatgaagc
    29041 taaaggcgga agaagatgaa ctgattgctg tgtatctgaa agaagatcag ccaggcttag
    29101 atgttttctt agtaacgcat caaggaatgg gtttgcgcta tcctttaaca gatgttcccg
    29161 ttgttggagc aaaggcagct ggagtgaagt ctatgaattt gaaagatgga gataggatta
    29221 tcaacgggct gcttgtgtta gcagaaggtg atacgccgat cgtaatcgtt actcagcgag
    29281 gagctgttaa acgcatgctt gctcaagaaa tcagccaaac ttcacgtgcg aagcggggag
    29341 tgacggtcat gcgtgagctg aaaaaaaatc cgcaccgtat tatctatatg tcggaaggaa
    29401 gatcaaaaga aataaccttg atgaatcaaa aagggcagca gttaacgatt gacccaactg
    29461 atttccctat aggtgatcga acatcgaatg gatcatttgc gatggatgag aaaaaaggtg
    29521 gagaagtcat cgaagttatc gatgctcctg agattttcat cgagtcgtaa ctgttgtagt
    29581 attttaatga agagacgcca tctgtattaa tacaggtggc gtctcttttt cattcatttt
    29641 taataaatcc ttatacgttt gcaaaaaaag atgaaagtgt tcacaatgct taaatgttgg
    29701 tatgtcaacg ttgatttaaa tatatgtaaa taaaacataa aaattgttaa ataaaaaaca
    29761 agaaaaccct tgcaaagata gggtgctgtg ttatactaat cacgtaaaaa ctgttacata
    29821 aaacagaaat ttaggaggtc catacaatgg agcaatggaa tggatttaaa ggtaccaaat
    29881 ggaaagaagg cgtagacact agagacttta ttcaaaataa ctacactgaa tatcgcggtg
    29941 atgacagctt tttggaaggc ccaacagaag caacagaaaa attatgggat gaattgcaaa
    30001 agttaaatga tatccaattc gagcgtaacg gtgtctatga tatggacaca gaaatcgtat
    30061 caactattac ttcacacgat gcaggctatt tggataaaga tttggaaaaa atcgtcggtc
    30121 tacagacaga caaacctttg aaacaagcat tcatgccgtt cggcggaatc aatatggcta
    30181 acaatgcgct aaaaagcaat ggctatacac caaacgatga attgacacat attttcactg
    30241 actggcgcaa aacacataat caaggtgtat ttgacgctta tactgctgaa atgcgtgctg
    30301 ctcgtaaaaa caaaatcatt acagggcttc cagacgctta cggtcgcgga cgtatcatcg
    30361 gtgactatcg tcgtgtggca ttgtatggaa tcgactattt gatggaacaa aagaaaaaag
    30421 atcatgatat gactggttat catacaatga gtgaagaagt gattcgttta cgtgaagaaa
    30481 tcactgaaca atatcgtgca ttagcgcaaa tgaaagaaat ggctgcgaaa tatggctatg
    30541 atatttctaa accagctgca aatgcaaaag aagctgtcca atggctatac ttcggatatt
    30601 tagcagcaat caaatcacaa aatggtgctg cgatgtcaat cggacgtatc tcagcattcc
    30661 ttgatatcta tatccaacgt gatttggata atggagtaat cacagaaaaa gaagcacaag
    30721 aattgatcga ccacttagta atgaaattac gtatggtcaa attcgcgcgt acacctgaat
    30781 ataaccaatt attctcagga ttccctatct gggctacttt atccattgca ggtatgggat
    30841 tagatggtcg ttcattagtt acaaagaatg atttccgtct gcttcataca ttaacaaata
    30901 tgggaccttc accagaacca aacttgactg ttttgtattc tgaacacctt ccagaaggat
    30961 tcagaacata tgctagcaga atcgctatcg aaagttcttc gattcaattc gaaaatgata
    31021 atcagcttcg tgaacaatgg ggttctgatg attgtgcgat tgcttgctgt gtatcaggaa
    31081 cagttgtcgg aaaagacatg caattctttg gcgctcgtgc taacttggct aaaacactat
    31141 tatatgccat caatggcgga gtcgatgaag tgactggcgc ccaagttgga ccagaatatc
    31201 gtccaatcac atcagaaata ctagactttg aagaagtgaa acataacttc atgaatatga
    31261 tggattggtt agcagaacta tatgtcaata cgttgaacat cattcattat atgcatgata
    31321 aatattctta tgaagcagct cagctggctt tgatggattc tgacttgcaa cgcacatttg
    31381 ctacaggtat tgctgggatc tctcatgcgg cagacagctt gtcagcgatc aaatatgcaa
    31441 aagtaaaagc aatccgtaat gaaaaaggaa tagcagttga ttttgaagtg gatggcgact
    31501 tccctaaata cggaaatgat gatgaccgtg cagatgaatt agtttcatgg atcttggaat
    31561 acttcatggg acaaatcaaa cgtcataaaa cttaccgcaa ctcaaaacca acaacttcat
    31621 tattgacgat tacttcaaat gttgtttacg gtaaaaatac aggaaatact cctgatggcc
    31681 gtgaagctgg aaaaccactt gcgccaggag caaatccaag ttacaatgcg gaacaaagtg
    31741 ggttattagc tagcctgaac tcaacagcta aaatgaatta cggctgtgca ttggatggaa
    31801 tttcaaatac ccaaacaatc aatccttcag gtcttggaaa agaacaagat acaagaatcg
    31861 aaaacttgcg taatgtctta gatggatact tctcaaaagg agcttatcat ttgaatgtca
    31921 atgtattcag taatgattta ttgcgtgatg caatggaaaa tccaatgaaa tatccgaact
    31981 tgacgatccg ggtatctggt tatgcagtta aattccgtga cttgacaaga gaacaacaat
    32041 tagatgtctt gatgagaact tcacacgaca gaatgtaaaa agttcgtgaa aaaagttttt
    32101 cgacttagcg tcgaaaggac gttgaatagc catcgcatca aaatcgaata atcagtatgc
    32161 ttatttgaaa gaaacgattt acaggggcag gacttgtatg ttccgtccct gtaaatttta
    32221 cacataacga agcggcaatt gcgaaaaatt ttgttcagaa agcttcgctt cgacagctaa
    32281 cttgtactta aaatgaaatc aaagaaaaga tgtgaactta aatggaagaa aaaacaatcg
    32341 gctatgttca ttctatcgaa actttcggtt cagtagacgg acctggactg cgctttgtcg
    32401 tgtttatgca aggatgccgc atgcgttgcc aattttgtca caatccagat acttggaata
    32461 ttggcggagg gaaagaatac acagcagatg aacttttaga taaagcagaa cgcttcagac
    32521 cttactgggg agataaaggt ggtattactg ttagtggtgg agaacctcta ttgcagatcg
    32581 attttttgat cgaactattt aaaaaagcaa aagagcggaa gatgcatact acattggata
    32641 catgcggaaa accttttacg tatgaagaac ctttcttttc acgttttcaa gaattgatga
    32701 agtatacaga tttactgtta tttgacatca aacacatcga taatgaagag cataaaaaac
    32761 taacgcattg ggacaatgaa aatattttag aaatggctca atatttgtca aaaatcaaca
    32821 aaccggtttg gattcgccat gtactagttc ctgagcgaag tgattatgat gaatatttga
    32881 tccgactaga caactttatt caaaccttgt caaatgtaga cagagtagag attcttcctt
    32941 atcatacgat gggaaaatac aagtgggaaa cacttggttt gaaatatcca ttagaaggga
    33001 tcgaaccacc tacaaaagaa cgtgtcgaaa atgctaaacg attgcttcat acagaagagt
    33061 atacaaaata tttaacgcga taggtcgcat cggaaagaag ataagtattg cttctttccg
    33121 tttttttgct attattaaat aggagaaatc acaaaggagc gaatgacatg tcaaaaattt
    33181 tagtttttgg tcaccaaaat ccagatacag atgcaatcgg ggcagccatt gcgttttcac
    33241 acttgcaaaa tgaattaggt aaagaaacag aagcagttgc tctaggtaca cctaatgaag
    33301 aaacacaata cgcattagac cactttcagc tatctgcacc acgagtagta gagtcagcta
    33361 aagcagaaaa tgcagatgtc atgcttgtgg atcataatga attccaacaa agtatttcag
    33421 atatcagcga agtaaatatt ttagctgtag tagaccacca tcgtattgca aactttgaaa
    33481 cagctaatcc attgtactat cgtgcagaac cagttggatg tacaagtacg attattttaa
    33541 aactatataa agaaaaccaa gtaacaattc ctaaagaaat cgcaggaatc atgctctcag
    33601 ctatcatttc agatacattg ttgttcaaat caccaacatg tacgcctgaa gatgaagcag
    33661 ctgcaaaaga acttgcagca atcgctgaag tcgatctaaa cacttatgga cttgaaatgt
    33721 tgaaagcagg aacaaatcta agtgataaat cagctgcggt tcttcttgat ttggatgcaa
    33781 aaagtttccc aatgggcagt tccaacgttc gtatcgctca agtcaataca gtggacctta
    33841 atgaagtgat ggatcgacaa gcagagctag aagcagcaat ggaagatgaa atgaaagcaa
    33901 atggctacga tctgttcgta ttagttgtca caaacatctt agacagtgat tcagaattac
    33961 ttgttgctgg taaaggaaaa gaaaaagtag aagaagcttt ccaaacaact ttgaccaaca
    34021 accgtgcttt gcttaaaggt gtcgtttcac gtaaaaaaca agtcgtacct caattgacgg
    34081 ctgtatttag ctaatcaaat ggtagtatga agcattcaaa atgaatggat aagcgattga
    34141 tataaaaaga aacatagaac atactaaagg ccgaagaaat ggaggagaat aacagtcaat
    34201 tatgactctt gttcttcctc ctttttacgt aaaagccttt taagtaaaat aaaacggata
    34261 gaaaagagaa catatatgaa ctattattca aaaaataagg aaaactttta tcaaaaactg
    34321 accgatgatc cgcttttttc ttcattgacc gattatctat atgaacatcg agaacaagaa
    34381 acgattctaa gagaattgaa aaaagaattt tcgcaaaata aattttccca ttttcttgat
    34441 ttattgatcg atgcaggatt aatcaagagg gaagaacgaa gatatcattt gaactttcca
    34501 atatttgatc ctaaagatta cttgcaacaa gccacttcgg ctgctgaaac catagctgaa
    34561 caattgaaga gactctctgt tgatgaacaa aaattagcga tgggagaagt tatttgggcg
    34621 tattgtttcg aagatgaacg gaaagaagcg tatttttatg gagtaagaaa cagccgagag
    34681 acagaattgc tgaggacaac tgcaggaaat gagaagtacc gttttatcac actttcctct
    34741 atagaacact tcccgcttac tttggctaat tacttttttg tacagaagaa tcagcttcct
    34801 gtaacgaagg cattcaaaga attagccgaa cttattggag acgtaaatga atcgtacttt
    34861 tttgatcaaa tagaagttat cgttgacagg atacgtaaga ataaatataa aaatcgccgg
    34921 cctagtattt tccatcagtc tctacttgtg acgaatacta taaaggaaga agaatcattc
    34981 acattagaat tacctattgt cgaaaaaaac aattttgaga ttgaattacc aacattggat
    35041 ccttcattga caatggagga aatggccttt cttaaacgac aaatcttttc tgagctgagc
    35101 aagaaattta taccgcatgc attttcttat atcaaagaat atggaacagt tttagtgagt
    35161 aaaacctaag agagcagtaa gcaaaaatta tactgactct tgcaggagga gcctttctct
    35221 tcctttatgg ttaaaagaat tatttgagta ttttgcctat ctaccaccta ttagattcac
    35281 tacatggtct ggaaacaaag gaatgcaaaa aaatggtgag aaatgtggaa tgacaaagga
    35341 agtagtgata agaaaagtta gatttttaaa taatcaatat tatgattcag tcaagtatgg
    35401 cattttatgg gaagaactag ctgattagat taggaagtcc atttgataat ccagaatcac
    35461 taagaaacga atggcgagtc attaataatt tatccaatct tgggttattc ttttagattg
    35521 aagtgtttaa aactaaaaaa gcatttatga agtcgtaata gtcaggcttc aaaatgcttt
    35581 ttattttgtt tgttttttct ttgccacata aatacaccta gactgacgat gaagagcata
    35641 tccaacagat ttcctgcgac ttggatcgtc gaagggttgg tcagtggcat taaagcaagg
    35701 acaaaaaagg aaaacaagct gtaaataaaa caggtgacag gaactaatcg aaagaagagc
    35761 ggacatttca attggctaaa aatgagtgaa aaaataaaat aagtaaaaat ggataaaatc
    35821 acgactaaac cattcatgat aaagaagaaa agcgtattgt gattaaacga ataaaaaaga
    35881 attgctaaaa aagaaaacgt tttaaatgca atcaatacgc ttgaagcttc gtttaacgta
    35941 ccaggatgtc gatgaaaaaa ataggcaccg aaataaacag aaaacagtaa gccgcaaaga
    36001 gaagacaaaa tcaaaaaaaa cggattttct tcggcttctg tgatatcaga atgatccaaa
    36061 agcaaccagc ctaatgcact agaaggcgca tgtgaggtct taaggtatga ataacataga
    36121 aaaaataaga tcgtcaaagt tgcaaaaaaa gagcctaaat gtaaaaaatt agtggttcta
    36181 cgttgaattt ccataatatc tccaaattaa attgataagt atataaagta
//
LOCUS       DAFGZE010000024        34600 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.024, whole genome shotgun sequence.
ACCESSION   DAFGZE010000024 DAFGZE010000000
VERSION     DAFGZE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.024"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(43..891)
                     /locus_tag="LTU54_001245"
     CDS             complement(43..891)
                     /locus_tag="LTU54_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL8295968.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1115..3055)
                     /gene="thrS"
                     /locus_tag="LTU54_001246"
     CDS             complement(1115..3055)
                     /gene="thrS"
                     /locus_tag="LTU54_001246"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBL8295969.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3392..3571)
                     /locus_tag="LTU54_001247"
     CDS             complement(3392..3571)
                     /locus_tag="LTU54_001247"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HBL8295970.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3618..5441)
                     /gene="cadA"
                     /locus_tag="LTU54_001248"
     CDS             complement(3618..5441)
                     /gene="cadA"
                     /locus_tag="LTU54_001248"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL8295971.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5719..7998
                     /locus_tag="LTU54_001249"
     CDS             5719..7998
                     /locus_tag="LTU54_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8295972.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8091..8738)
                     /locus_tag="LTU54_001250"
     CDS             complement(8091..8738)
                     /locus_tag="LTU54_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8295973.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8917..9636)
                     /locus_tag="LTU54_001251"
     CDS             complement(8917..9636)
                     /locus_tag="LTU54_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8295974.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9767..10918)
                     /locus_tag="LTU54_001252"
     CDS             complement(9767..10918)
                     /locus_tag="LTU54_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL8295975.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11135..11875)
                     /locus_tag="LTU54_001253"
     CDS             complement(11135..11875)
                     /locus_tag="LTU54_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8295976.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11879..12202)
                     /gene="rsfS"
                     /locus_tag="LTU54_001254"
     CDS             complement(11879..12202)
                     /gene="rsfS"
                     /locus_tag="LTU54_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBL8295977.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12259..12852)
                     /gene="yqeK"
                     /locus_tag="LTU54_001255"
     CDS             complement(12259..12852)
                     /gene="yqeK"
                     /locus_tag="LTU54_001255"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBL8295978.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12836..13486)
                     /locus_tag="LTU54_001256"
     CDS             complement(12836..13486)
                     /locus_tag="LTU54_001256"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBL8295979.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13496..13807)
                     /gene="yhbY"
                     /locus_tag="LTU54_001257"
     CDS             complement(13496..13807)
                     /gene="yhbY"
                     /locus_tag="LTU54_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBL8295980.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13823..14932)
                     /gene="yqeH"
                     /locus_tag="LTU54_001258"
     CDS             complement(13823..14932)
                     /gene="yqeH"
                     /locus_tag="LTU54_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HBL8295981.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14929..15456)
                     /locus_tag="LTU54_001259"
     CDS             complement(14929..15456)
                     /locus_tag="LTU54_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HBL8295982.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15636..16421)
                     /locus_tag="LTU54_001260"
     CDS             complement(15636..16421)
                     /locus_tag="LTU54_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBL8295983.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16414..17283)
                     /locus_tag="LTU54_001261"
     CDS             complement(16414..17283)
                     /locus_tag="LTU54_001261"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBL8295984.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17284..18663)
                     /locus_tag="LTU54_001262"
     CDS             complement(17284..18663)
                     /locus_tag="LTU54_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBL8295985.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18671..19090)
                     /gene="fabZ"
                     /locus_tag="LTU54_001263"
     CDS             complement(18671..19090)
                     /gene="fabZ"
                     /locus_tag="LTU54_001263"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL8295986.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19115..19591)
                     /gene="accB"
                     /locus_tag="LTU54_001264"
     CDS             complement(19115..19591)
                     /gene="accB"
                     /locus_tag="LTU54_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBL8295987.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19594..20829)
                     /gene="fabF"
                     /locus_tag="LTU54_001265"
     CDS             complement(19594..20829)
                     /gene="fabF"
                     /locus_tag="LTU54_001265"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBL8295988.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20848..21585)
                     /gene="fabG"
                     /locus_tag="LTU54_001266"
     CDS             complement(20848..21585)
                     /gene="fabG"
                     /locus_tag="LTU54_001266"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBL8295989.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21597..22514)
                     /gene="fabD"
                     /locus_tag="LTU54_001267"
     CDS             complement(21597..22514)
                     /gene="fabD"
                     /locus_tag="LTU54_001267"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL8295990.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22621..22845)
                     /locus_tag="LTU54_001268"
     CDS             complement(22621..22845)
                     /locus_tag="LTU54_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBL8295991.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22898..23860)
                     /locus_tag="LTU54_001269"
     CDS             complement(22898..23860)
                     /locus_tag="LTU54_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBL8295992.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23870..24310)
                     /locus_tag="LTU54_001270"
     CDS             complement(23870..24310)
                     /locus_tag="LTU54_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8295993.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24616..25581
                     /locus_tag="LTU54_001271"
     CDS             24616..25581
                     /locus_tag="LTU54_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBL8295994.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25638..25868)
                     /locus_tag="LTU54_001272"
     CDS             complement(25638..25868)
                     /locus_tag="LTU54_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HBL8295995.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25954..26841)
                     /locus_tag="LTU54_001273"
     CDS             complement(25954..26841)
                     /locus_tag="LTU54_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBL8295996.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26930..28153)
                     /locus_tag="LTU54_001274"
     CDS             complement(26930..28153)
                     /locus_tag="LTU54_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL8295997.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28170..29216)
                     /locus_tag="LTU54_001275"
     CDS             complement(28170..29216)
                     /locus_tag="LTU54_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL8295998.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29380..31107)
                     /gene="ptsP"
                     /locus_tag="LTU54_001276"
     CDS             complement(29380..31107)
                     /gene="ptsP"
                     /locus_tag="LTU54_001276"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBL8295999.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31107..31373)
                     /locus_tag="LTU54_001277"
     CDS             complement(31107..31373)
                     /locus_tag="LTU54_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HBL8296000.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31852..34086
                     /locus_tag="LTU54_001278"
     CDS             31852..34086
                     /locus_tag="LTU54_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8296001.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34234..34554
                     /locus_tag="LTU54_001279"
     CDS             34234..34554
                     /locus_tag="LTU54_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL8296002.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPQH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
BASE COUNT         9772 a         7484 c         6040 g        11304 t
ORIGIN      
        1 ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg aatccataaa
       61 atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt cgtttttagc
      121 catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg aattagttgc
      181 cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat cggctatctt
      241 gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca tgcttttcgc
      301 cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa gccaaagcac
      361 tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt tttcaaaaat
      421 gattggctta ccgacacgag accatttttg cagaatatct tctactacta aagaagaggc
      481 gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga ctttttggat
      541 cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat tgttaccaag
      601 tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac cttcatcgat
      661 tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt gaaaaccgat
      721 ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg ctaattcatt
      781 agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca actttttttc
      841 tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca taagttcctc
      901 caccctttta atgatagtct atgtaagtat accatatcag acccttattc acggtcgtat
      961 actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca tcaatcccac
     1021 agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt cggattgctt
     1081 tctatctact acttttttac aggaaattta cctattagtt ttctctgctg taatttttga
     1141 cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt tctttacttc
     1201 cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca actaattggt
     1261 aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca ttacgatcat
     1321 caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa gcataatcgc
     1381 catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac catgttggaa
     1441 aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta gaaacaattc
     1501 cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca tatgtcaaat
     1561 caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct tctgtaccaa
     1621 gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg ccttctgctt
     1681 caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg gctttttccc
     1741 acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag ctcaagcgga
     1801 aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca agcgtacgtt
     1861 taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc aacgtcattt
     1921 ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc atcattccta
     1981 attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg tagaccatca
     2041 tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc atatccattg
     2101 gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac aactcaacat
     2161 ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg tctacgatat
     2221 aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc aaaccagaac
     2281 ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga tggtctcttt
     2341 cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca aaaaaggctg
     2401 tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa gcacctgcca
     2461 ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt ccacgacaca
     2521 aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc aattcactaa
     2581 tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt tttgaaagaa
     2641 cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct tcgattgccg
     2701 gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa aaccctgaat
     2761 cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg gcttgggcca
     2821 tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct ggtgtgatga
     2881 tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt ccgttcactt
     2941 taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt gccaatgttg
     3001 ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt atcataatag
     3061 tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa aaaaatccta
     3121 gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa tgttcaaacg
     3181 caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca agggtttcga
     3241 aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc ctctcttatc
     3301 gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat ttaaccacta
     3361 ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca tttacaccgt
     3421 atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt gcctgcgtgt
     3481 ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc ggactgcgac
     3541 cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct gatcgttctt
     3601 ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg aaaaatggct
     3661 ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac ttcttgcaaa
     3721 acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa aaaaatacaa
     3781 ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct tgaaatcact
     3841 actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc actttcacta
     3901 gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc aagtgctggt
     3961 gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg gatactttct
     4021 aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa ttggactttt
     4081 tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa atttttcaca
     4141 tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg atccgaaaat
     4201 aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt ggacgtttct
     4261 atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt attgactact
     4321 gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag ttggctgttt
     4381 ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc tgcactagct
     4441 gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc ctctacttcc
     4501 aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt acgtgcgagc
     4561 ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg gctcattcct
     4621 gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac taccagtacc
     4681 tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat caaataagca
     4741 atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc aggcttttct
     4801 tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc acgtttttca
     4861 gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg atctccaggt
     4921 ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga agattctcct
     4981 ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa ctggtcatca
     5041 atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg atgggcaatt
     5101 cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt cgcgtaatcc
     5161 tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca gtattctttg
     5221 acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata tcttcctgaa
     5281 cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc agtaaatgcg
     5341 acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa agctatccca
     5401 ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc tcctctttta
     5461 tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta agcatagtcc
     5521 ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac tatcagcgtt
     5581 tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt ttcgattcat
     5641 cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt tatttacggc
     5701 ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt ggatgaaacg
     5761 atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga attgactcag
     5821 gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg ttccaatgaa
     5881 gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct tttgaaatac
     5941 catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa agatagtcct
     6001 tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata tctaggtatc
     6061 gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc accaattgcc
     6121 aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac agatagaata
     6181 gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat tttatctatg
     6241 gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga tgaatcatca
     6301 agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc aatcattcgg
     6361 gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa gacatccgct
     6421 ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga ggctgaaaac
     6481 gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt attgccttca
     6541 ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca gctgctgcca
     6601 gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg ggaaaaagat
     6661 ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg ctatatccaa
     6721 ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg tacgatccta
     6781 tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc tttacatcat
     6841 agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca aaaagacgaa
     6901 acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact atacgcaaca
     6961 gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa actggccaac
     7021 caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta tcaatttgtc
     7081 aatataacga aacaatatct ccactttcta caaactgtgc cactttctct tctaacacga
     7141 cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac acacttgcgt
     7201 caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg ccgaacatat
     7261 ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca ggatttcgca
     7321 ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac tttctgtgga
     7381 gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga agaccttttc
     7441 gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac aaaagaaatc
     7501 actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac agcaagaaaa
     7561 ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa ttggttagca
     7621 gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat catagctaaa
     7681 acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa acaacaagtc
     7741 cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact tccagccttt
     7801 ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc agaacatttt
     7861 tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat gcatgaattg
     7921 acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat ttcatcttat
     7981 gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc agttgtcttt
     8041 ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat ttattctata
     8101 tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat cattttcagt
     8161 tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc aatcccgcca
     8221 ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga ttggaaattg
     8281 ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag gtatgcacta
     8341 tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag aattttttca
     8401 ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg atactcttct
     8461 tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag ataattttgt
     8521 ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt cagataccct
     8581 gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta tttttgcgct
     8641 aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta accgcttaag
     8701 atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt ccatcttcca
     8761 catacttcat atcatttcac atgatactta tgctctcatc ataccacagg ttgaattaga
     8821 aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt cttgcttttc
     8881 gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg ccacattatg
     8941 gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt caaatgtttc
     9001 ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct cagcgattga
     9061 aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat ccgctacttc
     9121 accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga tgtctttttc
     9181 cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga ttgctgtatt
     9241 gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt tcccaaatgc
     9301 tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa caatcaccat
     9361 tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat ctcctgcacc
     9421 ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag ttttagcgcg
     9481 atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg attttgctgc
     9541 cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat ctttagcagt
     9601 tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct ttctgatttg
     9661 atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg attctgtgaa
     9721 gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat actcggattg
     9781 ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt tgatcacttc
     9841 taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa atagaaatct
     9901 cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt cgaagataat
     9961 ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca taacaagata
    10021 atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca aaatcctcaa
    10081 aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg gtcatctgat
    10141 agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa tacggccaat
    10201 aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga ggcggcacaa
    10261 gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca ctggctatcc
    10321 tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc ggatgtaatt
    10381 ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag atatgatttg
    10441 gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca gtcatttttt
    10501 gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc ctatcttgat
    10561 tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct tctgttccaa
    10621 aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta gcaaaatagt
    10681 cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca ccattttgta
    10741 aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt tgcaaaaaat
    10801 tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc atttccacat
    10861 gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag cttttcatgc
    10921 gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa aaggcaaaaa
    10981 tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt ctgcgacgaa
    11041 aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg ttgaagattt
    11101 attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct acaaagaacc
    11161 agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct tcgactttcg
    11221 aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta cgctcttgat
    11281 gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg aaaaaggtaa
    11341 gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa aaagcaaaat
    11401 tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga taggtggagt
    11461 gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca tcaaacactt
    11521 gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag gtaattgcct
    11581 gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg atcgatgcgt
    11641 cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg tctaaccctg
    11701 tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg catgccagtt
    11761 ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac cattgctgat
    11821 aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa gccatatgtt
    11881 aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt tccagattat
    11941 agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga tcgatcaata
    12001 cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt tcttcttctt
    12061 tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct gaacagatca
    12121 tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg tcttctgccc
    12181 gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct atgatgattt
    12241 cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt cgattgtttt
    12301 tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg tttcaaatgc
    12361 tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc caggaaaatc
    12421 tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac tcatcttagc
    12481 agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga ttcctaattc
    12541 ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat aattcaataa
    12601 ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg gtcgttcttt
    12661 tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac cgtatttttt
    12721 ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt gttcgctcat
    12781 tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt cattactata
    12841 gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct ggaacaaggt
    12901 agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg atgtcgatct
    12961 caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct ggtcgacgta
    13021 tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt ggcaaatatt
    13081 ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt tgcgtcaatt
    13141 ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc gtttcaatct
    13201 gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt tttgcatcaa
    13261 tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat acgcggtcaa
    13321 gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga acgggattga
    13381 atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc atttcttcag
    13441 gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc actccttata
    13501 ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt ttgtataata
    13561 caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct aatacttgag
    13621 ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc agttctcttt
    13681 tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct tttccgattt
    13741 gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt tttcctctta
    13801 aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca tctacaccct
    13861 ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct agaccggcaa
    13921 aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc ggaaatgcct
    13981 cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga taaaattcgt
    14041 ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa gcaataaatg
    14101 aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa agtgtttgcc
    14161 cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg atccgcaaat
    14221 cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt gtatcaatca
    14281 aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct ggaaactgcg
    14341 aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg ataagcgtcg
    14401 attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg tattcttcga
    14461 ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat aatacatcta
    14521 ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat ttagtttttt
    14581 tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga ttgtcaccga
    14641 caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc accacattga
    14701 cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg tcatccgtca
    14761 atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga caatagactt
    14821 ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat tcaccgggtt
    14881 tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc attcgattcc
    14941 acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc ttttttccca
    15001 aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta caaggatgct
    15061 acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc ctaccattac
    15121 tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc ttgctaaagg
    15181 tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg cgacacgttt
    15241 tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt ctaaaatcca
    15301 agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat tgtctaaatc
    15361 ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct gatagatggc
    15421 ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc tgcaaaaaat
    15481 gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc aaaaaaaact
    15541 acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac caaaaataaa
    15601 gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa cctcttgtat
    15661 cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct ttcaatcaac
    15721 gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca
    15781 ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac
    15841 tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga
    15901 gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca
    15961 agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca
    16021 gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca
    16081 ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa cttttccgct
    16141 tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg
    16201 cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca
    16261 tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg atcaccatgg
    16321 cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta
    16381 gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg
    16441 tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg gaacaatctg
    16501 atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg
    16561 cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc
    16621 tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt
    16681 taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt
    16741 tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa
    16801 caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc
    16861 gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg
    16921 aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg
    16981 cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc
    17041 cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc
    17101 gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata
    17161 aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt
    17221 agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc
    17281 catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa
    17341 aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat ccatctgaaa
    17401 ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat
    17461 taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata
    17521 tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca tcccacctgc
    17581 tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc
    17641 tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt
    17701 caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat
    17761 ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc
    17821 ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc
    17881 tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga
    17941 ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc ctaaatgaat
    18001 gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag gataaatgat
    18061 tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga
    18121 aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc
    18181 tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc
    18241 tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa
    18301 ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa
    18361 tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa
    18421 tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt
    18481 cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt
    18541 atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg
    18601 gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa
    18661 catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta
    18721 ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa
    18781 ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga
    18841 aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag
    18901 ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa tgtccttgaa
    18961 aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt
    19021 cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt
    19081 gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg
    19141 gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg
    19201 cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt
    19261 tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt tgcagataaa
    19321 cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt
    19381 tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg
    19441 gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat
    19501 acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac
    19561 tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag
    19621 aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg
    19681 tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat
    19741 cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc
    19801 ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa
    19861 attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact
    19921 tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc
    19981 aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca tatggtaagc
    20041 gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc gagcttgtgc
    20101 atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa aaccattccg
    20161 attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa
    20221 agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat
    20281 gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct
    20341 agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca aagcaatatt
    20401 tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc
    20461 tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc
    20521 gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat
    20581 tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt
    20641 tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt
    20701 ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt
    20761 caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac
    20821 tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt
    20881 gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc
    20941 ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc
    21001 tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat
    21061 gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact
    21121 agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag agagattaat
    21181 gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt
    21241 tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc gcatcacaag
    21301 tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc
    21361 tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac
    21421 gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc
    21481 attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc
    21541 tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat
    21601 agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt
    21661 ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt
    21721 tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact
    21781 tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt
    21841 atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt
    21901 gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt
    21961 ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca
    22021 atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct
    22081 tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg
    22141 cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct
    22201 tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct
    22261 aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact
    22321 gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa
    22381 agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg
    22441 gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag
    22501 aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc
    22561 aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga
    22621 ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc
    22681 tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa
    22741 gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt
    22801 tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc
    22861 cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg agaatacttc
    22921 cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt cctaaggtga
    22981 tctttccact agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc
    23041 catatttatc catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt
    23101 tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca
    23161 tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag
    23221 tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct
    23281 ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca
    23341 attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag
    23401 cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca atcaccagtc
    23461 ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg
    23521 agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt
    23581 gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca
    23641 aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat
    23701 cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc
    23761 attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa
    23821 ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt
    23881 cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag
    23941 ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata
    24001 gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga
    24061 tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag
    24121 taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct
    24181 cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc agctcagact
    24241 cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat
    24301 ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa
    24361 ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct
    24421 ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat
    24481 tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg aacagaacta
    24541 tagaattccc taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc
    24601 gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc
    24661 tatcttatga aagaccgaga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa
    24721 aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg
    24781 attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg
    24841 ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat gaattatttc
    24901 agtaattata attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg
    24961 gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg
    25021 gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagtccag
    25081 cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aggaactcca agggctgttt
    25141 caaggccgaa ataaaaagct actgacttac ttgaaaaaaa gacaacaaca tttcatctat
    25201 cagttgaatt tcgaaaaagc cggtatgctt caaaaagata tcgaattagt tacttatttt
    25261 attagacgga tccaggaaca aaagcagttt ttaagaacac catcgctcac cttcagcatg
    25321 ccattagcag cagatgagag tcagaaaaag cactacctcg tttgttatgg tcaacttgca
    25381 gaaacaatga ttacctctgg tgacgtttct cctgacttct attatgaaaa gaaagaagct
    25441 catttatctt tgaaacgtca attgtcaaaa gaagaaatcg atcctgtaca aatcttgatc
    25501 agttaccgca aaaaattaga aaaagaacag tttgaaattg aactgttaaa caaaaaagaa
    25561 gctgaaaagc aactaaatta gttgcttctc ggcttctttt aggcatcttc aacttcaaat
    25621 gtcaattatc ctaaaattta ttggattaag tcatagattt ccatcgctac gatatcgata
    25681 ttatcaaatt cgtattgctg atgagtatct gtttcagtta attcaaatgt atcggtcgct
    25741 ttatcataag ttactgtaca cttctctacg ccttcttttt caaaacggcg aacttgcagt
    25801 tcattatctg ctgactcgac catagcctcc agtcgtttta tgattgcgac taattgtgaa
    25861 tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat tgtatcaaaa gcatagccta
    25921 cttgcattga ttttctctaa aaaaactcgt tttttatgat tttttttctg gtatttttcc
    25981 ttcaggccac caaagtttga tcaaagcttg ggttccgtca ttctcaaaac ctttatctgc
    26041 caactcttca tatagtttca atgcttgggt agttgctggc aatacaagat ccattttttc
    26101 tgcttcttct aatgcaatct ttaaatcttt gataaaatgt ttgacaaaaa aaccaggtgt
    26161 gtaatcttct tttaaaatac gaggagaata attgcttaaa gaccagttag ctgcacttcc
    26221 acctgaaagc gtctcaatca ctttctttaa atcaagtccg tttttttgtg catagacaag
    26281 catttccgtc attcctgtca tcgtacctgc aatcatgatc tgattagcca tttttgtatg
    26341 ctgacctttc ccagcagaac catgcaatgt aaatgtcgta ccaaattctt tgaataaagg
    26401 cagtacttga tcataaacgg cttcttctcc accgaccatg atcgtcagtg taccattctt
    26461 tgctcctaag tcaccaccgg ataccggtgc atctaacgca tctgcgcctt tttcttttgc
    26521 tgtttttgca attttttctg ccaggcttgg tgtacttgta gtcaaatcaa ctaaaatatg
    26581 tcctgagata tctgcagaaa aaatccccga ctgtccatag tagatttctt ctacatcttt
    26641 ggggtaacct accattgtaa agagaatatt actttgttct gctaccgctt ttggtgtatc
    26701 tgcccagata gctccttctg ttactaaagg atcagctttt gcttttgtcc gattatagac
    26761 agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca gcacttccca tgacgcctgt
    26821 tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa tcaaaaatcc ttgcttctat
    26881 gctagtatac gctagaaaca aggatttttt cataaaaaat gtttgaattt tatcttttaa
    26941 atgaatttaa ttttattttg attcgttcaa tcgagtcttc ttcttctttt cttcgcatct
    27001 cttcttcatg ataaacaacc atgtcttgat aaaattcgat catagcttcg ccgaaatgtg
    27061 tagatgaaat atcataaagt ttagcttcta aaacttttgg atctttagga acttcactgg
    27121 aaatataatc aataagtgct ggtgcaaagt cttcgtcggt ttcaaaagtt ttccctaatg
    27181 aaggatgatc gaacaatcgg ttcaaataag catttccttc cacgacacaa ggtgttccgg
    27241 ctgccatcgc ttctgtataa gtaagccctt gtgtttctga agtagaagca ctgacaaaat
    27301 aatctgccgc tttataataa agtgctacat cattgttgtc cacttctcct gtgaactgca
    27361 caaattcttc taactgcagt tctgagatca attcctctaa ctcttctaca taaggtcctt
    27421 ttcctacgat aactaatctg gcgttaggat aagcggagat gacctgagga aacccattaa
    27481 tcaaggcttg gatatttttc tcataagaaa tcctgcttaa agaaagaatc atcaaatggt
    27541 tttcctgaag acctagtgac tcccttaatt tgctgatatc ctgctgagtg atatcagaac
    27601 gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg aagcggtgct attacaccat
    27661 agctgcgtaa tgtatcaata acacgttcac ttggacaaac tacacctgtc gtatgatttg
    27721 caaataatct agacaagtat tttacatggg attgcctgat cacttttcct ttaccgatat
    27781 aatgaaggta gtcttcatac atcgtatggt aagtatggat cagaggtatt ttcaacttct
    27841 tagctaccat cttcccaagt atccctgctc cgaattctgt atgggtatga ataagatcta
    27901 gttctagttc tttggcaacc aaataagcat accacatgcc tctaacaaca actcttcgat
    27961 ccttgaaaga aataaatggt acacttggca ttcgaatcac atctttttcc aacttatccg
    28021 cattaggatc ggtcgttgta aaaatataga cttcatgccc atgtctttct aattcttgtt
    28081 ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg aaagtaggta tcggtaaaaa
    28141 agccgatttt catgtttttc gcctccaaac tagtccttga cctgatttac tttttttaat
    28201 ttgctagaga ctttttcatt cagtacactt tcgtatacat ttttaagttc ttcaccgatt
    28261 ttattgatac ttcgctcttt tgctgtttga taaccggctt gtgtcgtact cggtagttct
    28321 tgattggtaa tctgttggat cagtcggata aattcttcgt ttgtcttccc catatagcag
    28381 ttcttgctat cttctaacca gccattatac actggaatat ctcgaacaag tacgttttgt
    28441 ttgcttgcta aagcttctaa aacaacgatc ccttctgtct cttcataaga agggaaaaag
    28501 aatagatctg cacctgaata cgcaccttct atgatctctc cacgaatgta accaggaaac
    28561 aggacatttt caggatgatc ttcttttaca attttccgga tgttccgagg aatcgagtac
    28621 atgggaacat gaccaaacca aataaatgtg tattcgggca tcttcttagc aatttctacg
    28681 aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac aaatgattac tttttgatcc
    28741 ggagacagtt tgaaatattc tctgaacttt tgttctttca ttggatcagg gtgatatttc
    28801 tctaagtcga tcccattgga aatagcttga atcggcacac ggataccata gctttccaac
    28861 aactgttttg aataaggtgt tggcgtgatc agatagtccg ctttttggta caatcctacc
    28921 agatattttt ttacgatagg agaaagctgg ttcgatccga taaaggaatt tcggaaatct
    28981 tcctctgtag aatgcgcatg atagacaact ttttttcctt ctcttctggc tttattgacc
    29041 atattatgac tattgattcc gtatgtattg atgtgcagga tgtcataatc ttctttttca
    29101 tctagtgtat aagagatacc gacttccgtc agagcacgtt tttgatgatc aagcgcacgc
    29161 ccgattccag attttgcaag catcttttca ctttcaaaat ataataatac cttcacctta
    29221 cgtccccctt cacttatata gtataacata gcctacaaac tgtcactcaa acttaagccc
    29281 tatttttgtc tattcttact gacaaaaacg ttttcttaaa aaagaacaaa aaaactggcg
    29341 tagctctttt acaagccaca ccagcttttg atacgttgtt tattttacat attcatgaac
    29401 aagttcaacg acttcttcca tcgtatcgca ttctttcaat gcacgatcag caagttcagc
    29461 cattttcgct gtgtccaagc gtttcattaa actacgcgtt ttaagaatag atgtcgcact
    29521 cattgagaac tcatctagac ccatacctac tagcaatgga acagctgttt gatctccagc
    29581 catttctcca cacatacctg cccattttcc ttcagcgtgt gctgcatcaa ttacattttt
    29641 gatcaaacgt aaaattgatg ggttatatgg ttggtacaag taagaaacgc gttcgttcat
    29701 acggtctgct gccattgtgt attgaatcaa atcgttcgta cctacagaga agaaatctac
    29761 ttcttttgca aacttgtctg ctaagactgc tgctgctgga atctcgatca tgatgccgac
    29821 ttggatcgta tcagatactt ccttgccttc acttatcaat ttttgttttt cttcttcaaa
    29881 gatcgctttt gctgcgcgga attctttgag tgtagctacc atagggaaca tgatacgtaa
    29941 attaccatga acagatgcac gaagcaatgc acgcatttgt gtacggaaca tttcgtcgcc
    30001 ttgttcagat aaactgatac gcaatgcacg atatcctaag aatggattca tttcatgagg
    30061 taattgcaga tatggtagtt ctttatctcc accgatgtcc attgtacgaa caactacagg
    30121 ttttccttcc ataccttcta atacagcttt gtaagcttca tattggtcat cttctgttgg
    30181 gaagtctgga gaatccatat acaggaattc agtacggtac agaccaactg cttctccacc
    30241 attattatgg acaccaacta gatcttttgg tgttccgatg tttgcagcta attcaaaatg
    30301 cttgccgtca gctgtgacag tttctgcatt ttttaatttt tcccattctg ctttttgtgc
    30361 tgcgtaatct gcacctgctt tttcaaattc agctttttgt tcatctgtag gatcgatgat
    30421 tacatcgcct tcgatcccgt tgacagccaa aatatcgcct tctttgactt tagcagtaat
    30481 ttcttttgtc ccaacgattg ctggaatttc taaagaacgt gccatgatag cagaatgtga
    30541 agtacgtcca ccaatgtctg ttacaaacgc ttttacaaaa ttgcgatcta attgtgctgt
    30601 atcacttgga gtcaagtcat gtgcaacaac gaccacttct tcgttgatca ttgatggatt
    30661 tggtaaagta acgcctaata agtgcgctaa aatacgttta gcaacgtcac gaatatctgc
    30721 agcacgctct tgcatataag cattatcttc cattgcttcg aacataccga tatacatatc
    30781 cgttacttct tttagcgctg attcagcatt tacgctgttg tctttaatat tttgcttgat
    30841 ttgacctacc atttctggat ctgaaagaac catcaaatgg gcatcaaata cttgcgcttc
    30901 tgcttcacct aagctttgcg ctgctttttc acgaatttgc tgcaattctt cagtagattt
    30961 agctaatgca gcatccaaac gagcttcttc agccgcagta tcttctactg ttgccttact
    31021 gaatgttaaa tccggttgaa ctagcaggta agcttttgca acggctacac cgtcactagc
    31081 ggcaatccct tttagcattt caaccattat tcagataagc cttctttctt cattgtttca
    31141 acgattgctg ccattgcatc agcttcgtca gcaccttctg cagtaatagt aacatcagaa
    31201 ccttgaccta cacctaaaga cataacgccc atgattgatt tcaagtttac tgatttacct
    31261 ttatactcta agtttacatc agagttaaat ttgcttgctg tttgtactaa tagtgtagct
    31321 ggacgagcgt ggatcccagt ttctgctact acgtgaaatt ctttcttttc catattaatc
    31381 ggtctccttt aagtgagtgt tgtttttatg tgagggtttt cttttttagt aattcagaga
    31441 atcctaacaa atgccaccct ttcatctgta aagattaaca ttttttttcg ttagatacaa
    31501 cagttttcat tcaatttcag ataaaagtat tcaaattatt ccggattctt tttcatcctt
    31561 ttcttcacat tttataacaa acgtgtttat ttcctttttc ttgtcgcttt ctcttctcct
    31621 gacgtttatc attctttcct ttgaccctct ttgttttcat atgaaacgaa ttacttgctt
    31681 ccctttttat ttttagtata ataagagtca aaggtcagga atggtcaata gagatttcac
    31741 cttttctacc tcataaatct cttaaaaaac taggggttat tcttttacct catgaaaaac
    31801 gagatgtaaa atatggatgt acccattttt gtgaaaggac gtgaatgatt catgctttgt
    31861 caaaactgcg gcaagaatga ggcaacgatt cacttgtatg ccactgtcaa tggtcagcgg
    31921 acacaactcg attactgtca gagctgttat caaaaaatca aaaatcaaca aaatggaggt
    31981 ctgactaata tggcccaaaa tgatccattt ggatttggca gtttggacga tcttttccgt
    32041 tccatgtctc gccaaatgca acagcaagga aattatgagc aaacaccacc aacacaattt
    32101 ggcggaaaca acaacttcaa tggtggtcag ccacctcaag gcaatacaga aggtttattg
    32161 ggagaatacg gaatcaatat cacagaacaa gcacgacaag gtgatatcga tcctgtgatc
    32221 ggacgcgatg acgaaatcaa acgagtcatt gaaattttaa atcgccgaac caagaacaat
    32281 cctgtcttga ttggagaacc aggtgttggt aaaacagcag ttgttgaagg tttagcacaa
    32341 aaaatcgtag atggtgatgt accgcaaaaa ttaatggaca aagaagtcat tcgtttagat
    32401 gtcgtatctt tagtccaagg tactggtata cgaggacaat ttgaagaacg tatgcaaaaa
    32461 ttgattgaag agatccgtca agcagaaaat gtgatcctat tcatagatga agttcacgaa
    32521 atagtcggtg ctggttctgc tggtgatgga aatatggatg ctggtaatat tttaaaacca
    32581 gctttagctc gaggagaatt gcaaatggtt ggtgctacta ccttaaatga gtaccgcatc
    32641 atcgaaaaag acgctgcttt agaacgtcgt atgcaacctg tacgggtaga tgaaccaact
    32701 gtcgaagaaa cgatcagtat tttgaaaggt ttgcaaaaac gttatgaaga ctatcatcat
    32761 gtaaaatata ctgatgaggc catcgaagca gccgcaactt tatccaatcg ttacgtccaa
    32821 gatcgcttct tgccggataa agccatcgat ttgctagatg aaacaggttc taagaaaaac
    32881 ttgacgatcc aaatcgttga tcctaaaaca atcgagaaaa aactgcaaga agcagaagaa
    32941 caaaaagttt tagcttctcg agaagaagat ttcgaaaaag cagcttatta tcgcgatcag
    33001 atcaataaac ttcaaaaaat gaaagaacgt cagctcactg aagaggaaac acctgtgatt
    33061 accgaaaaag atatggaaaa aatcgttgaa caacggacag gtattccagt gggtgaatta
    33121 aaagaaaaag aacagacaca actgaaaaac ttagcagatg acttgaaagc tcatgtcatc
    33181 ggacaagata acgctgtaga tcgagtagcg aaagcgattc gccgaaaccg tgttggatta
    33241 aacaaacaga atcgtccaat cggctccttc ttatttgttg gaccaactgg tgtaggtaaa
    33301 acagagcttg cgaaacaact tgcctacgaa ctattcggtt cacaagattc aatgattcgt
    33361 ttcgatatgt ctgaatatat ggaaaaacat agtgtctcta aattgattgg ttctcctcca
    33421 ggttatgtcg gatacgaaga agctggtcaa ttaacagaaa aagtacggag aaatccttac
    33481 agtcttgtct tgttagatga agtagaaaaa gctcatccag atgttttgca tatgttcttg
    33541 caaatcttag atgatggtcg attgacagat gcacaaggaa gaacggtcag ctttaaagat
    33601 acgatcatca ttatgacaag taatgccgga actggtaaag tagaagcaaa cgttggtttt
    33661 ggtgcggcac gcgaaggtgt tactcgttct gtattgaacc aattaaacaa ctactttaca
    33721 ccagaatttc tgaaccgttt tgacgggatc atcgaattca gtgcattatc aaaagaaaac
    33781 ttgatgacaa tcgtcacact aatgctagac gatgtcaacc aaatgctggc agcacagcaa
    33841 cttcacatcg aagttcctac gaatgtcaaa gaaaaattag ttgacttagg ctatgatcca
    33901 tctatgggtg ctcgtcctct tcgacgcacg atccaagaac aaatcgaaga tggtatcgct
    33961 gaattttatc ttgatcaccc ttctatccat gagttgaaag caaaactgga taaagatgga
    34021 aaaatcatcg ttacttctaa accagaacgt ttggctaaag aatcagccga agaaacggct
    34081 gaataaatcc agttttatag cgtgagacgg tatttctttg agaaatgccg tctcattctt
    34141 ttttcagaaa agtcaaaata ctgttcattt tataaaaaag aaattataat aagagaaaca
    34201 agcaattaaa aatacataaa agaggtgagc gccatgaaat tggtaaatgt aacaaacagc
    34261 cattcccgtc ttgtattaaa ccagcttgaa aacactgatg cccatctagt caaggtctac
    34321 acagccggaa ataccactat tgtctacact gaagcccctc aacataatga aattctgctg
    34381 atcaatgaca aacgtaaaat ccagccgaaa gaaatcgaag atgctaaaaa ttatttctta
    34441 gataaaatca aagacgccgt ttaccacgag gatgaaatca aagtaatcga attggcaggc
    34501 cttgttgaaa tctcgattcc aaaacatgaa ttaggcgcta cattagccgt gtaaacaaat
    34561 tttgaacata ataattaaag agcgtgggac aaaagtaaag
//
LOCUS       DAFGZE010000025        34544 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.025, whole genome shotgun sequence.
ACCESSION   DAFGZE010000025 DAFGZE010000000
VERSION     DAFGZE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.025"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(257..2149)
                     /locus_tag="LTU54_001280"
     CDS             complement(257..2149)
                     /locus_tag="LTU54_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296003.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2146..3873)
                     /locus_tag="LTU54_001281"
     CDS             complement(2146..3873)
                     /locus_tag="LTU54_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296004.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3889..4539)
                     /locus_tag="LTU54_001282"
     CDS             complement(3889..4539)
                     /locus_tag="LTU54_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL8296005.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4743..5039
                     /locus_tag="LTU54_001283"
     CDS             4743..5039
                     /locus_tag="LTU54_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL8296006.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSLTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5211..5831
                     /locus_tag="LTU54_001284"
     CDS             5211..5831
                     /locus_tag="LTU54_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBL8296007.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5923..7089)
                     /locus_tag="LTU54_001285"
     CDS             complement(5923..7089)
                     /locus_tag="LTU54_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL8296008.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7101..7454)
                     /locus_tag="LTU54_001286"
     CDS             complement(7101..7454)
                     /locus_tag="LTU54_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL8296009.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7582..8604
                     /locus_tag="LTU54_001287"
     CDS             7582..8604
                     /locus_tag="LTU54_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HBL8296010.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8623..8784
                     /locus_tag="LTU54_001288"
     CDS             8623..8784
                     /locus_tag="LTU54_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296011.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9043..9477)
                     /locus_tag="LTU54_001289"
     CDS             complement(9043..9477)
                     /locus_tag="LTU54_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296012.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9587..11446)
                     /locus_tag="LTU54_001290"
     CDS             complement(9587..11446)
                     /locus_tag="LTU54_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL8296013.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11564..12724)
                     /locus_tag="LTU54_001291"
     CDS             complement(11564..12724)
                     /locus_tag="LTU54_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HBL8296014.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13031..14455)
                     /gene="araA"
                     /locus_tag="LTU54_001292"
     CDS             complement(13031..14455)
                     /gene="araA"
                     /locus_tag="LTU54_001292"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBL8296015.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14471..15169)
                     /locus_tag="LTU54_001293"
     CDS             complement(14471..15169)
                     /locus_tag="LTU54_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL8296016.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKYY
                     LRKHGKNAYYGQNK"
     gene            complement(15126..16787)
                     /locus_tag="LTU54_001294"
     CDS             complement(15126..16787)
                     /locus_tag="LTU54_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HBL8296017.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVSGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17005..18405
                     /locus_tag="LTU54_001295"
     CDS             17005..18405
                     /locus_tag="LTU54_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBL8296018.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18657..19736)
                     /locus_tag="LTU54_001296"
     CDS             complement(18657..19736)
                     /locus_tag="LTU54_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8296019.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19822..20781)
                     /locus_tag="LTU54_001297"
     CDS             complement(19822..20781)
                     /locus_tag="LTU54_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBL8296020.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20793..22304)
                     /locus_tag="LTU54_001298"
     CDS             complement(20793..22304)
                     /locus_tag="LTU54_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL8296021.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22326..22889)
                     /locus_tag="LTU54_001299"
     CDS             complement(22326..22889)
                     /locus_tag="LTU54_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HBL8296022.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22895..23758)
                     /locus_tag="LTU54_001300"
     CDS             complement(22895..23758)
                     /locus_tag="LTU54_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296023.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23772..24668)
                     /locus_tag="LTU54_001301"
     CDS             complement(23772..24668)
                     /locus_tag="LTU54_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8296024.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24843..26132)
                     /locus_tag="LTU54_001302"
     CDS             complement(24843..26132)
                     /locus_tag="LTU54_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8296025.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26104..27621)
                     /locus_tag="LTU54_001303"
     CDS             complement(26104..27621)
                     /locus_tag="LTU54_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HBL8296026.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27819..28709
                     /locus_tag="LTU54_001304"
     CDS             27819..28709
                     /locus_tag="LTU54_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HBL8296027.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28782..29606)
                     /gene="nadE"
                     /locus_tag="LTU54_001305"
     CDS             complement(28782..29606)
                     /gene="nadE"
                     /locus_tag="LTU54_001305"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBL8296028.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29618..31090)
                     /locus_tag="LTU54_001306"
     CDS             complement(29618..31090)
                     /locus_tag="LTU54_001306"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBL8296029.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31727..32089)
                     /locus_tag="LTU54_001307"
     CDS             complement(31727..32089)
                     /locus_tag="LTU54_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HBL8296030.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32312..33064
                     /locus_tag="LTU54_001308"
     CDS             32312..33064
                     /locus_tag="LTU54_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL8296031.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33204..34127)
                     /locus_tag="LTU54_001309"
     CDS             complement(33204..34127)
                     /locus_tag="LTU54_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296032.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34142..34414)
                     /locus_tag="LTU54_001310"
     CDS             complement(34142..34414)
                     /locus_tag="LTU54_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296033.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10189 a         7203 c         5949 g        11203 t
ORIGIN      
        1 agctcctcct atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
       61 gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag tattagagat acaaaacgga
      121 aaaatagctt ctcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
      181 accgtttatt cggaaaaagg gactgtgaca cgacttttgt cacagcccct aaaaaaaata
      241 ataaatttgt actattctat gaagcttctt cttcagaaaa ctgactgtta taaagatctg
      301 catagaatcc gtctttttcc atcaatgtat cgtggtttcc tgtttctacg atcgaaccat
      361 gattcatcac aataatgttg tctgcatcgc gtattgtaga caagcggtga gcaacaacga
      421 aacttgtccg gttttccaat aggcggttca tcgctttttg aatcagtatt tctgttcgtg
      481 tatctacact agatgttgct tcatcaagga tcaggacatc tggatttgct aagaatgccc
      541 gcgcaattgt gattagctga cgctgccctt gcgaaatatt gcttgcatcc tcgtttaata
      601 cagtgttata tccttctggc aatttacgaa caaaatcgtc tacgtgagca gctttagcag
      661 cctcaatgat ttgttcgtct gttgcatcgt cgttcccata tttgatattg tcatagatac
      721 ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa ctgtgcgcgc aattcatcac
      781 gtgaaagatc acgggtatct acaccatcgt aacgaatact acctccagaa acatcgtaga
      841 accgttcaag aaggttgatc agtgtcgtct tccctgcacc agtcggacct acaatagcga
      901 ccatctcacc aggtttgaca tccaagttaa aatcagtcat tagcaagtta tctggtgtgt
      961 agccgaattg aacattttca aaacgaactt tgtatggtgt atctttttct tctggaaggc
     1021 cagatttttc attggacatc tcttcttcat ccaacacttc aaatacccgt tcggctgaag
     1081 caactgttga ttggatagtg ttcatcaaac ttgcaagttg agtgattggt tgagagaact
     1141 gtgttgtata ttgcaagaac gcttgaacat cccctaatgt catgttccca tttgcaactt
     1201 ttactccgcc tagtacagcg acgaacacat agcctagatt tttaacgaaa ttcatcaacg
     1261 gcatgatgat tgccgaaata aattgagctt tccaaccagc ggcatacaga ttctgatttt
     1321 ctttttcaaa tacttcctgg tctttttcag tatgattgaa tgttttaaca atggtatgac
     1381 caccatatgt ttcttctact tggttattca ataaacctaa acttttttgc tgatctgcga
     1441 aaaacacttg tgatttcggc gctacgatca tcacgatgat caagcttaaa gggatcatca
     1501 ataacgcgac taacgtcaat tgccagctga ttgaaagcat catcacgatg accccaatca
     1561 atgtgactgc actagttaca aattgagtca aattttgctg cagcgtgctt gcgatgttat
     1621 ccatatcatt gattgctcga gacatgatat ccccatttgt atgtgtatca taataggaaa
     1681 tcggtacacg attcattttg gcttccaaat catgacgtaa ttcataaact gttcgttggg
     1741 aaacacgtgt cataataaat tgctgtaaaa agttgaatac agcagaaatc aaatacatca
     1801 cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc tatcggaaat tttgttactt
     1861 gttggccttg ttgcatctgt tgagcgccag ccattacacc tttgaatatt tctgtggttg
     1921 ctttccccaa gacctttggt gtttggattt ggaagatcac ggctgctatt gctagtacaa
     1981 atacagcgat gatcgcaacc atacgtttag acatatagcc aaacaatcgg cgtaccgttc
     2041 cccagaaatt tttaggcttt tcacctttta caccgagatt tctgccaggt ccaccatgtc
     2101 ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt tttactcatg ctaagtcctc
     2161 ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg tttgttgcta atagttcttc
     2221 atgtgttcct ttgccaacca gttttccttc atctagcaca aggatcatat ctgcatccat
     2281 tactgtactt actcgttgag caactaaaac ggtgatactt tctttcatat tcgttttcaa
     2341 tgcttggcga agattggcat ccgttttgaa atccaacgct gagaaggaat catcaaacac
     2401 atagatatct gctgttttga ctaatgcccg tgcaattgcc agacgctgtc tttgtccacc
     2461 agagaagttt cctcctcctt gttccacatg gctatctaag ccatcttcca tctctgaaac
     2521 aaaatcttta gcttgtgcga tttctaatgc tttccaaatc atttcatctg ttgcatccgg
     2581 cttaccatac tgcatattgt ctcggatcgt tccagagaac agaactgctt tttgaggaac
     2641 aaagcccatt aattctcgca aattatattg agtcatgtca ctaatgtccg ttccatttat
     2701 ttggatcgaa ccagattcga tgtcatataa tcgagggatc aaattgacta aggtcgtttt
     2761 acctgaacca gtccccccta tgattgcgac gatctcgccc gacttggctt ggaaatcaat
     2821 atcttccaat gcaagttttt ctgctccatg atatcgataa ttgacatgat tgaaggccaa
     2881 tgttgcgttt tttccagcaa aagaaactgt tttaggattt tcagggtcat ggataccgat
     2941 tttttcatcc aacacttcat taatacggtc agctgaagct tgtgcacgag gcaccatgat
     3001 aaaaatagcg gaaagcatca taaagctgat caagatctgc atcgcataag tcataaatgc
     3061 aatcagatta ccgacttcca aagtcatatc tgctatgtag tgtccaccaa accaagtgat
     3121 tgctacgttt gtaccgctca tgatcaatgt catcattggc atcatcaatg cgacgatcgt
     3181 attaactttg attgccgtat ttgtataatc tttattcgct aaatcaaaac gattttcttc
     3241 aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct gtcaatcctt ctcgaaatac
     3301 caggtttaaa cggtcagttt tcttttgcat acttttgaat aatggaacag caaagtacat
     3361 gattccgccg atcaaaataa tcattactgg gatgacaact aagaagattt ttgtcaactg
     3421 atgatctttt tgataagcaa gtatgctcgc gccaatcaac gtgattggtg cattgatcat
     3481 catacgtaaa aacatttgtg taaccatttg gatttgagtc acatcattgg tcgttcttgt
     3541 gatcaaagag gccgttccat atttatcaaa cgcatctttt gtcagactct ctgatttttt
     3601 atagacatcc gtacgcaatt gcttacccag tttctgtgat tcacgagtag caaaaaatgt
     3661 atttgcgata gcagctaaga tactgatcaa agaaaagccg atcatcacaa aacctgtatg
     3721 ccaaatataa tcgacatctc cttgcgctac ccctttatca ataatattgg aggtaagtgt
     3781 cggcaaatat aaatctgcca gtacttggat caccatgaaa actgcagctg caattgctga
     3841 agtcaaggag atccgtttta cgatttttag catctagttc ccttctttct atttatatac
     3901 tccgtttttc agccaagaaa gtttcaaatt gaattcactg attgcttgct ctactgagta
     3961 atcttcagat gttgaaaata agcgatacga atgcgcaatt gttgtaaata cagtattcat
     4021 cactaattgg atcagtaatt ccaattcatg atcatttttt actttcagct tcgtccgatc
     4081 gatcaattca agcaactctt ttgcatcttg tttgtgttct ttatgtggat cgtgaggcgt
     4141 tttttggcga ctcgtatgag gagcgacttt tcttgaggca cggtaatcca tgttcataaa
     4201 caagttacga taaaaagaag cattctcgcc ttccaaaatc tcataaatca ttttagaaaa
     4261 ataggattcg aatcctttga atagatcacc atcggattct ttcattagtt gtaacatatc
     4321 tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa taaagatcct ctttatcttc
     4381 aaaatactga tagaaactgc ctctagggat atccgctaat ttgacgatat tagcaatcga
     4441 agcttctttt aagggtactc tagaaaactc gattctcgca gcctcgatta gtcttttttg
     4501 tttttcttta ggcaaatgga aaaatgtctg tgtcggcata tggatcctcc tttctttcaa
     4561 gatgacacat tgtcatatga cgctgtgtca ttataacacc tcctacctgt tttgcaagac
     4621 atttgcatag tttttttcat tatcatccga tacattaaaa aagtgataag aggaatcctt
     4681 ccatttgaat taatgagagt tgcccattaa aatagccaca tactaaacaa aggatgagat
     4741 aaatggccca atcaattagt gtccaagact tcaataaatt gcctctcgat gctgacagca
     4801 tcgttttaga tacaagaaat accttagatt acacaaaaaa ccacttaagc cactcgcttt
     4861 ctatccccgc tgctatcttg ccaagacaat taaaaaatct agatccaaag aaactttatt
     4921 atattctaag ccttacaggc aggcgatcag aagcattagc cgatcaactt actgctaatg
     4981 gttttcgtgc tgtttatatc attggtggga tggaagctct tcagtcgagt gttgcttgaa
     5041 tattgcttgt tagattcaaa agaggctgtg acaaaaaatc gtgtcacagc ctcttttata
     5101 ccctgtcttt ttttagttat ttattcttca accttatcaa tatgacttga tagccttttc
     5161 tttttctcat agttataaca atcatagcaa taaaaggaga aggtaagcat atggcatctg
     5221 ttttagtagt caaaggtcat cccctaactg cagaagaatc gcgcacagtt aaggcattga
     5281 cgtctttttt aacaagttat aaagaaaatc atccagatga tgaagtgact gttttggaac
     5341 tttatcgcga tgatattccg gaaatcgatg aagaattatt atcaggttgg gaagcattac
     5401 gtgctggtgc agaattcact tccttatcag aaagtcaaca acaaaaaatt gctcgtttca
     5461 atgaattgac tgatcagttc cttgctgctg ataaaatcgt tatcgcaaac gccttatgga
     5521 atctaaatat cccaacaaaa ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa
     5581 cgttccgcta tacagaaaac gggccagaag gattagtgac aggtaaaaaa gcactgcaca
     5641 tccaatctaa tggcggtgta tacaacggac aagactttgc ctctcaatat gtaaaaggta
     5701 ttttaaactt tgtaggcatc gatcaagtag atcagctatt catcgaagga atcgattatg
     5761 accctgaccg tgcagatgag ttgatgcaaa atgcattaga taaagctgct gctttaggta
     5821 aatcatttta aaaattgaga taaatgcata caaaagagac agaggaaaaa atcttcaaag
     5881 cagcatttgt accttggata ttttcccatc tgtctctttt tgttattctt tctgcttttt
     5941 caacggattt tcttcataaa gagaagagat tgttgtgtac cattcaaaaa catgagtaat
     6001 cactacttta gctgctgcat aaacaggaat ccccaagatc acaccgatga ttccgaacaa
     6061 tttcccagaa gtcagcagaa ctaataaaat cgttactgga tggattgcta actgattccc
     6121 tagtactaat ggagagatca agcgtccttc tattgtctgc tctactgcaa aaacgatcag
     6181 tactttgatg atcatgaatg gacctgctac aatggctaaa aagatagccg gaatcatcgc
     6241 taaaaatgat cctaagtaag ggatcaggtt caaaaaaccc gcaataatcc ccaacgtgac
     6301 agcatactct aaaccaatga ccgcaaaacc aatcataaac atgattgcca cagcaaaagc
     6361 cacagttagt tgtcctctta tataagaaga aacttgacta ttcatttcac tcagcacatt
     6421 caacgtcggt ttttgcatgc gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt
     6481 accgtctttc aatagataaa ataagataaa cggcatcgtc agcagagcca ctaaaatagt
     6541 cgctaccgcg ccgacaaaac ttccaagact ctgaactgta ctcttagata tatcttggat
     6601 catatcgcct gccgaattca tgaacttatc acctgctgtt tccaactgtt cgcgaaattg
     6661 gttgaacagc gggtcccgca aaatctcggt taatttatta tcaatcgtat ctacatattg
     6721 agggaagtta ctgatgaagc tcactgtttg ttcctgaatc ttagggatga tcactacgct
     6781 cccccatgcg attagtgcaa caaccaaaac aaacagccca atgatactgt aaagccttgg
     6841 tacttttcgc ttttcaaagt aatcaactac cggattcatc aagtaataca agattcctgc
     6901 taaaatgata ggcagaccta cgattgctaa aaattgccag attggtgcaa acaaatatga
     6961 aacttttgta aataaaaaga cgatcagcaa tattaacaat acaacaagta aggctgtgac
     7021 tacttgatta tttaaaaacc agcgccaaaa ccacgatacc ggtctgttct tcttattttt
     7081 tccttccata attcctcctg ttagttataa aaaatttggc ttcccaaacc aatagcaggc
     7141 agtctatgcg gatgaagata gattccattt gcaatttgtc cttcttctgg acaagcatca
     7201 aaaatcaatg atacgtgagc tactgtctct aaattcccat tggccatcgg accgacataa
     7261 tcgatcgtat atacctgatc atcaaaagca attgttccgc ctgctttcaa agaaaaaggt
     7321 tcgtcttttg atacttcttg aatcaccgaa tattttcgta aggcagctgt tgctgtttta
     7381 tcgaacaaaa tcagcatggg ttcttccttg tttattgctt ctgtcccaat ttctgtaatc
     7441 tttgcctgaa tcattgcact ctctccttta attgctttca aaaacagtat aacacattct
     7501 cattctttag ccgtgtggaa taattatgtg atagaataaa aacgaaaact atttcattac
     7561 aattaggagg aatcagctca tatgatcgaa aagataattt taggaaccta tacgcgtcgc
     7621 gaaagtaaag gaatctatac gatcgacctt gatacagaaa aagaagaatt agccaacctt
     7681 tcattagctg ttgaagaaaa tagccctacc tatgtagcaa aaagtaaagc tggcaatctt
     7741 tacacagtta ctagtgtgga tggattaggt ggtgccggtg cgtacgataa ggattttcat
     7801 tttttgaatg cggtaacaga gtctggcgct cctttgtgtt atgtagcggt tgatgaagca
     7861 cgtcaattac tatacggggc gaactatcat aaaggtgaag taaatgtcta ccatatatta
     7921 ggagatggcg aactgaaagc agcagattca atattccatc aagaagcgac aggtcctcat
     7981 aaaaaccaag atcatgcgca tgtccactat acggatctga ctcctgacca acgattagtc
     8041 gtatgtgatc ttggtacaga tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg
     8101 aatcttgtaa ctaccttcac tgcagagcca ggaacaggcc ctcgccatct tgtctttcat
     8161 ccaaatggac aattcgctta tttatttggt gaattagaca gcacagtccg cgtgctttct
     8221 tataataaag aaacaggtgc ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa
     8281 ttcgacggtg aaaatggagg ggcagctatc cgcatttcaa atgatggcaa gttcctttat
     8341 gcttctaacc gaggacataa ctcgattgct gtattcgcag tttttgaaga tggttcccac
     8401 attcaaaaca agcaaatcat ctcaacagaa ggggattttc cccgcgattt cgcactagat
     8461 ccaagcaata attttgtcgt gtgtgccaat cagaacaccg ataatctgac attatatcgc
     8521 cgtcatcctg aaacaggatt actggaatta ctgaaaaaag atgtctatgt accagaatgt
     8581 gtctgtgttt attttgatcg ttagtaagaa aaggagaatg aaatgaatca gcaagacgta
     8641 ttagacagac tacgggaaga actaaatatt cctttctttg atgggaaaat cgaagataaa
     8701 gagtatagcg aagaggaata tcagaaactc aaagccgatt tacagaacta ctttgaacaa
     8761 tatgtacaga atgtagaaaa ctaatcagct ttccatacta tctgaaaaga acatcaggac
     8821 aagacttttc cgtctatcct gatgttcttt tctacaaata aaaaaccgcc gagatggtta
     8881 gactcgacgg aaaaggagta tttactcgat tttgagtaag tacaaggttg ttgttggtta
     8941 taggtatgtc attatcataa aggattttga tcactctatc ataaaggtaa tattacattt
     9001 atattagata actctagttt ttattgttat caaagatttc ttttattttt tcaacaacaa
     9061 attagtcatt ttttggatgg cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc
     9121 agatgtattg aatagatctt catctacccc tgattcaaga tcgatacttt ggatcatttc
     9181 ttcacagcct gctacatatg aaagataagc tttttcaaaa tttttatgaa tgcccattgc
     9241 ttttgctggt ggacgcaaag tactgacttt cttaagcatt gcccgatatt tttttgttcc
     9301 ttcttcaaat aacaggatga tctcttcaag agtcgatttg tccagttcac ttgtttttcc
     9361 ttcatcaatt gcgtttctga tcacttcata tttcggatgc attccttctc cgattgcttc
     9421 tgtttccttg acgatttgat cgatggactg cacataatag ccaatattcg gtaacatgtt
     9481 cgttttccta ctttctccat tgttgttatt atttattcta cttgttttta tcctcaaatg
     9541 acaagtactt gaaaaaattt aatggtttct atacagctcg ccattcttat tcgtgctcaa
     9601 agaaaccatc tgctaaacta tattgtttcc ctgcgacaat gtcatattgg atcaattgat
     9661 attcgtcgta aatctcttgt tgcgagcgat tgaattgcgc ttctttatac cactgtccgt
     9721 tttgatagta cagttctcgt tcaaacgccg gaagttcctg ttctaatgat aaaagcagct
     9781 gataaaagcc atttgttgtc tgattcgtct gttccattag attcgctgca aaataaaacg
     9841 gactggtgat tgcatcgtga gtcgtttgct tttctaattc tcctttactg tcgaacataa
     9901 gaaattgtgt ttcatgcaat gtttgtttgt cattttttgc ttggatcgta tctgaataga
     9961 tccctggaag atgatccccc caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt
    10021 cttccgtgaa ctgcttcagt gccgtgctgc tataggaaat atcttgcaga taattctcta
    10081 agtcaagtgt cccacttcca tctgaagttt ttgcaggata gtctagatta tcatactttc
    10141 catcgtaggg catatgtgtc tgcatcgtga ctaaatgaat gaactgaggt gttttttctt
    10201 ccttgagaag tgtcattact tctttgtaag ctgactcatc agaaatatat ggattattct
    10261 ctatcgtatc cgtataggtc atcgtacgct cgctgatgaa ctggtcaaat cctaatgtct
    10321 gataaacatc ctctctttta tacatcgaag tattgtatgg atggatcgct gtcgtctggt
    10381 atgattgtgc gtttaacgct gaaacgatag aaggaagctg atccattttt ggtacaagca
    10441 tcgtgtaagg ggtggtgagt tgcgcattaa ataacgccat agaaaaacca gtcaatgctt
    10501 caaattcgat atttgctgtt ccaccgccat agttttgaga aagcatattg ccactatatg
    10561 tttgatcagc tacttcataa taatctgcca atggctcacc agatacttct acgccgttta
    10621 atcgactagg atctgagaaa ctttcactca tcacgaacac gatgttcggc gactcttcct
    10681 ctacagtttt atttttttca tcggctagct tctggtattt ttcagtgatt tctttgatct
    10741 tttctttcga atatccttca ggttcatcca ttggttctac cttcagattg tacaaaaacc
    10801 caccgataaa accagtgtta tagtagttca ttttctggct gtaaggtatc catagtgccg
    10861 ttttattata tgcttttcgc aacaaattat cgggattgtt gaaattactg atatacccta
    10921 gcagtccaat tgccacaagc agtgtcgtta atcggatcaa ttggaatcgc cttccttttt
    10981 tcaggcttcg aaacatgtac cagcaaaaca atcctatcac ggttcctgcg gcagctaaga
    11041 tcactgtaaa cagcatagtg ccggtcatat ctttcagcaa tccgatttca gtgatcatct
    11101 tcaaatcgtc tggataaatc ggttcttgcc gataactcat tttcatataa tttgcaaaac
    11161 ctaatacacc gatggctacg acatacgtaa gggaacctaa agggacagat cctgctagtg
    11221 caatcagaaa aataagaata atcagcaaga cgagacaggc taagaagaat ttttccgtat
    11281 gccaagaaaa agcaaatttc aacgcaaggt ctacggatag atcattttga caccactgca
    11341 aaaataaatt actaaaaaca acaagaaaaa agatactgat gattgctgct gttcctttta
    11401 aaatcttttt ctgtttggtc gaaagtctgc ttatatatct tttcaaatgc ttgctccttc
    11461 atttctatca ataaaagaat gttgcttctc ttattttagt tgtacgaaat tcgaaagtaa
    11521 agaacggaaa gaagaattaa tgaaatccaa atattctctt tctttatggc ctttctggtt
    11581 tctttaataa gcggaccttt cgtactccag aaattttttc tagctgttgt ttcacttgat
    11641 tgagctgttc aaaatcagta ctgtctatgt caatcaatat gtagacatgc tcattcttat
    11701 ggttacgcga tatatctgcg atcgctagct cgttctcatt cagcagagaa aatatctctg
    11761 ctaatatatt ggttttcttt tgataaaata tagtgaatcg aaacggcgct tggaaaaaca
    11821 ttcgtgcgga tggataattg accgcttctc taaccgtacc gaacaaaaga taatctttta
    11881 aggcttcaac tgccaaacgt ccgctgtctg ccaaagcttc ctgagtcgat ccccctagat
    11941 gaggcaacat aagtatacgg tcattttcta agaactcttc ttctggaaaa tcagtcaagt
    12001 atttagccag atgttgtttt tccaatgctt gcataacagc atatttatca acaatttcac
    12061 ttcttcctac attgataagt acagcatctt ttttcatttt ttctatattc gcctgatcga
    12121 tcaatccttt tgtatcttct gtcaacggca gcatgactac gatgtaatcc gaggtagaaa
    12181 gaactgtttc taaatctgct tgccgaacat aatccaactg ggcatcgcgg atagaataac
    12241 ccaagacatc catccctaag ctatagcaac ttagcgctac ttttgtgcct atcgctccaa
    12301 gaccaagaag accgatggtt ttgccttgca attctcgtcc cacataagca cttcttttgt
    12361 tttctgcctg ttctaaaata ttaggacctg ttagtgtttg taccatacga cttgcttcga
    12421 ttatcggacg ggaagataag agcaaacagc acaaaacaag ctcttttact gcattagcat
    12481 ttacacctgg tgtattcatg acgatcgtcc cgttttcaga agctttttca acgttgatcg
    12541 tgttcacccc aacaccagct cttgaaattg ctagcaaatc tggtgtaagc cattcatctt
    12601 tcactttcgt actacgaata actattcctt cggctggcaa atcatctatt attttgaaat
    12661 attccccttg taacttttct atctcttgtt ctggaatagc atcaatcaaa tgaacgtgat
    12721 acataaaacc ccctccttac taacaagtat ctcgaaattt ctgcaattag acaactgttt
    12781 accatttgac ttcctagaag caaaaagagc agttagagca gagtaggctg agtgataaaa
    12841 ataaggctat gacaagttac tgccataagc cttattttcg agagcaaaat attgagggtg
    12901 agatatcttg ataaatggtg ttccgttagc tgacattgat aataagacta aatttcaaaa
    12961 aagttgaaaa gcaaatttcg tcaatctctt cttatttctt aaaactaagt ggctttttca
    13021 caacctcttt ttatctaata accactgttt ccaactcaac taattttgcc cacgtttcaa
    13081 tttgatctgt tgttagattt agcgagacaa cggtatgatg accgccacct gcctgaatcc
    13141 atgaatggac accttcatgg aaattcggtt tagttttcca taacacacgt gcaacaggta
    13201 aattaggtgc ttcagtcgtt ggttcaaagg cttctacttc attgatcagc agtttataat
    13261 gcgtaccgaa atctgccatc gatacgacta ccccttctcc tgctttccca tcaaatacaa
    13321 gacgcgccgg atcttctcta tctcccattg ataaaggaga aacaacgatt cttggtttcg
    13381 tcgcagcaag cgtcggatct acttccatca tatgggattc caagattgct tcttggccac
    13441 tagctagctc gtaagtataa tcttccatga atcctgtatc tttccctcga gccatgattt
    13501 tcagcaaccg atcaatcgct gcagttttcc agtcgccttc cccagcaaaa ccgtatcctt
    13561 ctgccatcaa acgttgtaca gctagtccag gtagttgttt cattccatac aggtcttcaa
    13621 aattagtcgt aaaagcgtta tagcctcctt cttctaagaa acggcgaata ccgatttcct
    13681 gctgagcttg tacttttaca tgttcctccc atgtcttttc ttcataatca ccatagtcga
    13741 aggtatacaa ttccttgtac tcttcaaata attgatcgac ttcttcgtct gatacgcgat
    13801 cgattacttg tacaagatct ccgataccaa aataatcaac agtccaacca aattgaatct
    13861 gtgcttccac tttgtctcct tctgtgacac ccacgtttct catgttgtca ccaaaacgag
    13921 ccacttttat tccaaaactt tcattatagg ctactgcaac atccatccaa tcagcaatct
    13981 ctttttggat ctcaggtcgt ttccagtagc caacgacgat tttattttgt ttatttaatc
    14041 gagcattgat gaacccatat tcgcggtcac catgagcgga ttgatttaaa ttcatgaaat
    14101 ccatatcgat cgttttccaa ggaatgcttt cgttatattg cgtagctaaa tgaagcaatg
    14161 gtttttgtag aagctttgtc ccgcggatcc acatttttgc tggtgagaaa gtatgcatcc
    14221 aagtgatcac acctgctact tcttctcgat aattgacctc tttcatgatt tttgtgatcg
    14281 tatcagcagt aactgctaat tcttgaaggc ggattggata aggcaattgg ccattttcat
    14341 tcagttcatc gaccatttct tgtgcatgtt tctttacttc tcgcagtgct tcttccccat
    14401 ataaatgctg tgatcctaca acaaaccaaa attctttttc tccaatattc aacataatta
    14461 gccactcctt ttatttgttt tgtccgtaat acgcattttt tccgtgtttt cgtaaataat
    14521 atttatctaa aatccgttga ggcagctctt ctgaaaatga attgagctgt ctggtaaata
    14581 aattcatctt gcatacttca tctagtacga ctgcgttcat taccgcactt tgcgcatctt
    14641 tcccccaagt aaatggaccg tgtccgtgta aaagtacccc tggaattgct aagggatcaa
    14701 ttttgcgttc tttgaatgtt tctatgataa cttttcctgt ttcatattcg tacccgctgt
    14761 cgatttcctg ttgtgtcaaa aatcgagcac aaggtaccga tccataaaaa gtatctgcat
    14821 gcgtcgtccc catcgcagga acatccaatc ctgcttgtgc ccagatcgtc gcccatgtcg
    14881 agtgagtatg gacgattcct ccgatttgag gaaatgaacg atataaaaca gcatgagtag
    14941 gcgtatccga agagggattg agtttacctt caatgatatg attgtccaag tccacaacga
    15001 ccatgtcgtc tgctgtcatc tcttcataac tgactccgct aggtttgata acgaaatatc
    15061 gtgtttcagg atcaaaagcg ctcacatttc cccatgtata tttgacgagt ccgtgtttag
    15121 gcaagtcaag atttgcttgg tatacttctt ctttcagttg ttccagcatt ttctatcact
    15181 cctttttctt gcaattgatg aatcgctgtt ttttcaatgt cgatcccctc acggtaacgt
    15241 ctcataaata tctgatagcc tgctatttct tctgctgaag gtgtgatggt gattccttgg
    15301 tcttgtccaa acacgtcttc tgccaagaag ttttctaaag acttcccatg tcgatccatc
    15361 atataggctg ctaataacgc gattccccac gctccacctt ctccggctgt gtccatcact
    15421 gtaactggtg cctccatagc agatgctaga atccgctgtc caacttctgg tgttttaaaa
    15481 atccccccat gccccactaa acgatcaatt tggacattct cagatttcaa tatatccatt
    15541 ccgattctca ttgcaccaaa agcactggct aaatggatcc gcatgaaatt cgctaaatca
    15601 aaacggctac ctggtgtacg tacaaataat ggccttcctt catttactcc tgtaatgttt
    15661 tcaccggaaa aataaccata agaaagtaat cccccgcaat ccgtatcgcc ttctagcgct
    15721 ttgttgaata ataccgtgaa taatttatct ctgcttatct ccatacccaa agaattggta
    15781 aattcttcaa aaatactgac ccaagcattg atatccgaag agcagttatt cgtatgaacc
    15841 atgcctacta gttctcctga aggtgtagtg accatgtcga tttccggatg gacattttct
    15901 agttcttttt ctaatacgat catagcgaag gcggaagtac cagccgaaat gttccctgtc
    15961 cgtttggcta cactgttcgt agcgaccata ccggttcctg catccccttc tggcggacaa
    16021 accgggattc ctggtctcaa atttcccgta gggtcgatca gataagcccc tgattcgctt
    16081 aacttgccag cttcttctcc tgatactaga actttaggca aaatgtgatc caagtcaatc
    16141 gctacgtgcg ctttttcgat taactgacca aaaatatttt tcatttcttg atggtaggtt
    16201 ttcgttgaag gatcgatagg aaacatgcct gatgcatctc ctacgcctag tactttctca
    16261 ccagtaagct gccaatggac ataacctgca agcgttgtca aaaaattaat ttctttcaca
    16321 tgttcttctt tattcaaaat agattgatac aaatgagcga tactccatct ctgcggaata
    16381 ttgtattgaa atttttccgt taatattcgt tctgcttctt cagtgattgt atttcgccaa
    16441 gtacgaaatg gtactagtaa ctgttccttg ctatcaaatg ctagataacc gtgcatcatt
    16501 gcactgaacc caatcgaccc gattgtcgta agattctcgc catagatttc gaatacttct
    16561 gctgccattt ttcggtaact gacgcgcagt cctttccaaa tatcttctaa tgaataggtc
    16621 cagataccat tttctagttt gttttcccac tcataactac cagcggcaat aggagtaaaa
    16681 gatgaatcaa tcaatacagc tttgatccta gttgatccaa actcaatccc taaagatgta
    16741 cgaccatcta tgacatctaa catactttgc gcatttactt ccttcataat gatttaactc
    16801 cctccttctc gtcaaacctg atcaaaccgt ctttttagta gcgcttacaa tgattattgt
    16861 atacttttgt acgtacatgt caagcggtta tttacaagaa tacaataaaa aacacttgtt
    16921 ttgtacgtac ataagttata aaatgaaatt gcttacaaaa tctgatcaac cgccttctcg
    16981 aaattctaga aggagagaat acttatgaaa aaagaaaaaa aagtttcctc atcgtttatt
    17041 tatttttttg gcgcatttgg cggcattcta tttggatatg atattggtgt tatgaccggc
    17101 gccttacctt ttttacagca tgattggggt ttggcaggta aagcttcttt gataggatgg
    17161 atcacgtctt cggtcatgtt aggtgcgatc cttggcggct ctttatctgg tcttctttct
    17221 gataagcttg gacgacgaaa aatgattctt ctttctgcac ttatttttat ggctggttct
    17281 gtcctttctg catctgcccc tcacaatggt tcttattttc tgattgcagc aaggattctt
    17341 ctaggtttag ctgttggcgc tgcatctgcc ctagttcctg cttatatgtc tgaaatggct
    17401 cctgctagac taaggggaag attgtcaggg atcaatcaag tgatgattgc ttctggcatg
    17461 ctcctttcct atgttgcaga ttatttgcta aaagtgttgc ctgaaacgat ggcttggcgc
    17521 gtgatgttag ggctcgctgc tgtccccgcg ctgattttat ttttcggtgt tttagctcta
    17581 ccagagtcac cgcgttttct tatgcaatct ggccgcttgg aagaggcaaa aagagtattg
    17641 aactatattc ggacaccaaa agaagctgaa caggaattcg aacaaatcca gctgaatgtc
    17701 aaacaagaaa agacaaccgg aacttcttgg catactttgt ttttagaaaa atatcgttcc
    17761 ttagtcattg ccggtattgg tgtagcagta ttccagcaat ttcaaggagc taatgctatt
    17821 ttttactata ttccgctgat cgtagaaaaa gcgactgggc atgcagcgag cgatgcattg
    17881 atgtggccga tcatccaagg aatcatttta gttgctggtt ctttgctctt tttggttatt
    17941 gcagacaagt tcaatcggcg aactttattg aaaataggcg gtagcgtgat gggactttca
    18001 ttcatcctac cagctgtttt aggaactgta ctggatgctc atacaaattc gttgttgatt
    18061 ctcttgtttt tatgtattta tgtcgcattt tattcttgca catgggctcc actgacatgg
    18121 gtaatcatag gcgaaatatt cccactagct gtccgcgggc gagcatctgg cctagcctct
    18181 tcttttaatt ggattggttc tttcctagtt ggcttgcttt ttcctgtgat gactgcttct
    18241 atgtctcaag aaatcgtttt cggcattttt gggatcatct gctttttagg ggttttattt
    18301 attcaagaga tcgtaccaga aactcgtggg aaatctttag aggaaatcga acaaagtgca
    18361 tctaaaaaaa cttatcctaa acgtatttct atccatcatt cttaaagagg ttgtgacaga
    18421 agtggtcagc ttcaagaaat aagaaggaac cgcttagttt ccagtcacaa ggaacaattt
    18481 caaaaaacag cttctcatgt ttgatgaata ttgtgacttg tgaccagttg gtaggagaac
    18541 accgtttagt ggagaggtgg tgaaagaaac gttcagcctt gagcgatgac aaaagctctc
    18601 acaaacattg cgacgagtaa ccgtaggagc aataagaggc ggtaaaaacc cgtctcttat
    18661 ttttcgtttt ctttcactgt ttcccgttca actagtacag gttcatagag aatatttggc
    18721 tgtgcctgtc ctttttcaat cgttgtgacg atccaccgtg cggcatcttg tcccaacttt
    18781 tcttttggat gcgtcaatgt tgtcaatttt ttattcccta aatggcttaa agaagaatcg
    18841 tctgttccta cgatcgatag gtcatctgga atatgatagc cattttctgt caacttatct
    18901 agcattaaac tagctatttg atcgttatag caaatgatgc catctaaagc ttgttcttct
    18961 ttcaaccgtt gtactgtttt ttcagcgatt gtttctcgtg tttccgtggt gaaagtaatg
    19021 atatcttccg tcaaaaaacg cacattatac tgttcacacg cgctgataaa acctttcatc
    19081 ctgtattttc cttgaagatc gtcgattttt gtgatgaaca acagacgctg atgtcctttt
    19141 tttatcaaat attctgctgc cataaaaccg actttcacat catctaaaca aatataagag
    19201 gcatttaact cttcataagc agcattgatc atcaccatcg gaatgccttg ttctctcaaa
    19261 gcaacataaa gtgctaagtt tgggttatac tgattacttt tcgtaggctc aacgatcaaa
    19321 ccatctaccc cataagcaat cattttcttc aaacactctt tctccaattc gtgatcgtta
    19381 ttcgtactgg ctaaaagcaa tgaataccca gattctcgta attcattttc gattcctcgg
    19441 ataatcgacg gaaaaatata atcagagaga tatgtggtga tgacaccgat cgtttttgtt
    19501 ctgttcaatg gtgttttttc tttcttatag ctgtcatcta catatgtgcc cgaccctttt
    19561 tctttacgca gatagccctc atttaccaac acagcgattg cttgccgaat cgtatgtctg
    19621 ctgacgtcat atatttcttg cagcttgaat tctgagggaa tggttgtatt tacttcgtat
    19681 tcctcacgaa ggatcttttg ttttatctct tctgcaatat cttgatattt tgccatttgc
    19741 cttgcccctt tttatttgta cgtacatata tcataacgag tctgcaaggt ttattcaagc
    19801 aaaaatcaag gtttcttgtg ctcataaggc atacggaaca ggttcaccaa attctggaaa
    19861 tccttgctca ttccacgtaa agcattgcgc gttcgcgtgc cgattaggat tgtctaacgg
    19921 atcccctttt tggttcttct ctggtcgagc atgatagatc aaaatatcaa agcacccgtc
    19981 ttctgaaacc gtaaacgagt tgtgaccagg accaaattgc ttattttttt cagaagaaac
    20041 gaatactggc tccgataatt tatgccatga ttcttttaat aaaagattct tgtgctcatc
    20101 agcccacagc aggcccatgg catagttttc atctgttgca ctaccagaat acgtaatgaa
    20161 tattttccca ttgcgagtaa ggactgctgg accttcattt actgaaaaac cccgtttttc
    20221 ccattcatac tcaggtatag aaaggaggat ctgcttcccc tttaacgtcc aaggattatc
    20281 catttcggat agataaagat tagaattccc agtaatttca ggatcttgtt gggcccacac
    20341 ataatatagc tttccttgag aagagaaagt tgttccgtct aaactgaaag tttccctttt
    20401 cgtttttatt tgtccctttt ctatccaatc tgtattcatt ggatctatat gctgattttc
    20461 aagtacgaac atgcggtgat gatgagattc atcacgtata gttttatgat tgcttgcagc
    20521 aaagtaaata taccattttc cattgatata atggacttca ggtgcccaga tcaattcact
    20581 catcggtcct tcttcatgag cctgccatac aatcaggctt gcagcatgtt ctaattcatt
    20641 cagtgacttt gcccttctta attcaatcac ttggtagcct ggaaccgatc ctgtaaaata
    20701 ataataacca tctgtatgtt tatataccca aggatctgcc cgttgccgaa cgataggatt
    20761 attataaagt gtcttcatca taacaaattt cctcacattt tctttattct aattacattc
    20821 caagagtatt tttctatccc tacatttaat tcgtcatgtt tttctattga aaaatagttt
    20881 atttcaggtt ttattttttc tggttcttca cgtgtatttt tgtccaacag atcgctactg
    20941 gttaatgtgt agtgctctcc ttgatctagc aaattgaaat catgttcaat cgttatcttt
    21001 tgttgttcat ttgtgcgatt gatcaagaaa atagtcagct gattgccgtt atctacgatc
    21061 acactgtcca cataaggaac attttttatc ttttcacaat cataaacagg gcaaatgatt
    21121 tgttgtttca atacttgacc tttagcatat ttcgataagt gttgcatcac ataataagtt
    21181 ggattcctcc atgcttgtcc tttagcatct gtcagtatta aaggtattgt gtttactaac
    21241 aatgattgac aactaatctt tacgcgatct gcgtgtcgta aaatagccag ccctaatgaa
    21301 ccaaataata atgtatcttc catagtaaag taacaccaat cgatgggact accgacttca
    21361 aaatccctat gtcttttttc cggttgggca tgtacattcc attcatcaaa cgctagatac
    21421 attgttttct cacttctcaa atatcctttt acataatcac aagtagcaac gatttcttca
    21481 atttgacgat cgaatcgata agcttttgca aggtatgtcg ctgtatcatc acgctcattt
    21541 ttaggaggtt tagtcaagtc ttcttcatca tacttatcaa tatagttatg caaagctaaa
    21601 taatctacat tctcataagc ttcttctaat gcgatacgat cccattcagg gtacgatggt
    21661 aaacgtggcg tagagcttcc aactaagatt gtttcaattg aatcatccac taacttcatg
    21721 acttttgagg cttcatttgc taacctacca tattcataag cactcttagc ccccacttgc
    21781 catggaccgt ctagctcatt ccctaaacac cataatttaa cgccaaaagg ctttttttgc
    21841 ccatttttta ttcttaaatc actccagaag gttccttttg gaaaattaca atattctact
    21901 aaattttttg cttcatcaat tccttttgtt cctaaattca ccgtgaatat cggtttcgct
    21961 tgtacatgac tgatccattt aaaaaattcg tggagtccaa aagaatttgg ttctatacct
    22021 cgccatgcta aatcaatttt ttttggtcgt tgttctttcc tacctacagt atccacccaa
    22081 tcataaccag atgtaaagtt tcctccagga tagcgaatga cacctaattc taattccttt
    22141 acataagaga ggacatcttg tctaaacccg tcaccatcag ctgtttcatg gcccggttca
    22201 taaatccctg tatagacgac acttcccata tgctcaacaa atgagccaaa cagccgagaa
    22261 ctgatctctc ctaccgtaaa atcctctctg atttttactt tcacttcttc gtccaacagc
    22321 tttccctacc tttcatttcc tatagtcaag ataatagata caagtttcca tacgccacca
    22381 gcaaagataa aaataaaagg agggaaaaga aatatgagca gtacaaatcc taataacaat
    22441 acgcaacaaa gaatatttct caaaaaatca aaaaatactt tccatgcact ttgttcaaaa
    22501 atttgtaaaa atgtgatatc tttgttttca gaaatcacat atatccagga taaaagaaac
    22561 atggaataaa taagtaaaac tacatatcca ctaattgaaa gtacgattgt ctgttcttct
    22621 aataattgat taacaagaaa ataaataatg atcgttaaca gactaaggaa caagttctta
    22681 ccaaaatgtt gctttattcc ttgaaagaaa aaataaatga tatttcctga tccctcacct
    22741 ttccattgcg ccattacata aaaaacagca catgtagcag gcacgatagt aataacaggc
    22801 aaacacacga ttacccagag aatacctaat aagatgtaat cagaaaccac aatcaactgt
    22861 ccataaaatt tagtctcaac tattttcatc ttcctcactc tttcattcca gacatagctg
    22921 ttccttgaac aaaatatttc tgtgcaaaaa tataaaccac tagtaatggc agcattgaaa
    22981 taaccgttcc agccattaca ggtccataaa aagaaatgtt ctgccctgta aataaagcta
    23041 accctgctgg taaagttctc atttttgaac tacttgtcaa tatcaatgga taaagcaagt
    23101 cattccaact attcatcagc gtgaaaatcc ctaatgataa aagagtaggt ttgctcaatg
    23161 gcaacatgat tttcaaaaaa atattaaact cacttaatcc atcaatgcgt gctgcctctt
    23221 ccaaagaatc tggtaaagta ataaaaaagg aacgcatcat gaagatacca aatgcagttg
    23281 tcatacgagg aatgatcaaa cctgcataag tatcaagcat gccaagcaaa ttgacttcaa
    23341 taaatagagg aatcatgaag acttggaatg ggatcatcat tgtcagcaaa acgaaataga
    23401 acaaaactga ttttcctttg aaacgcatac gagcaaatgc ataccccgca agagaatcaa
    23461 agaatacaga agtgatcact gtccctccag caaaaatcac tgtatttttg atgtaatcta
    23521 ataaagggat cgttgtccaa accttaatat agttatctag ggtatagcta tctgcaaata
    23581 ttctaggcgg aaatgagata atgtcttttt cgtgtttaaa agaagaaata atcagccaca
    23641 aaaaaggaaa cgcaataacc aacaatataa ctagcatact aatcgttcga atgatttttt
    23701 taccactaac tgcttttcga ctattcacag taactgtaat cgagctttct ggtgccatac
    23761 aggtttccct cctatttcat ttgtttttct ttccgtgaca tgtacacatt agtggagata
    23821 gctatagttg caacaatgat aaacaaaacc gaagacagtg ctgatgcata gcccaattca
    23881 taaggtgctg taaatcctcg actgtaaata tattgaacag ctgtttctgt cttaaattgt
    23941 gggccaccag cggttgcaac atatacttgg tcaaacactt gcatcgaacc aatcagattc
    24001 gttaaaatac aaaaacctaa ggagggaacg atctgaggta gcgtaatgtt aaaaaactgc
    24061 tgtcgatttg ttgcaccatc catttgagca gattcataca aagagggaga gattccctga
    24121 atagcagtca agagaatcgt catagtaaca ccaaaattct tccaaacagt catgatggct
    24181 accgtcggca tagcaagggt tggatcgcga aaaaattgag ggatcggcaa gccgacttgt
    24241 gaaaacaagt aaggaatcgc ccctacattc ggatcaagca acatagaaaa aataatcgca
    24301 attgacgtca atgaacaaat aactggaatg taaaatgtcg aacgacaaaa acgattgaat
    24361 ctggtatttt tagataatgc tactgctgct aataaaccaa cgataatttg tgttggcgtt
    24421 tcgagtacgg tgaaatataa agaatgaatc aacgaattaa ctgctcgttc atcactaaat
    24481 atacgtgtga aattttcaaa acttacaaaa tttcttgatg taaagaaaat attcaaatct
    24541 gtaaaactaa tagcaaatgt tcctaaaagc ggtatgacag taaataataa taaaactaaa
    24601 aaagatggag ttaaaaacaa atacgcagca cgatcaggac gattccaata tttggtgaat
    24661 ccgttcatat agtccacctc atctttcatt ttgataaaat atacaatctt ggtttaaacg
    24721 aatctgaata agatatccga acaatcgctg tgatttttgc aataacacct atctgcaaat
    24781 actaggtcga tcattcagat atctgtatct tgccaacagt ctatcctgta taaagttctg
    24841 tactattttt gcagtaattg attgatggca tcatctgcat cgttcatgag tgtctcagga
    24901 tcttctcctg ctaataattt ttcaatcagt ggattgatga tatcgttatt gatcgcagag
    24961 atttgggtaa aacctggtaa atatggctct gcatagtcca tttgtttcga taattcagat
    25021 acaataggat cattttttac ttcttcatct tttgctactg aatccaagta ggcaggaaaa
    25081 ccattttcga tactccattt tttaccaatt tcagttgtat tccaatattt caaaaactca
    25141 tagacagctt cttttttatt attatctgta cttgccggaa tggcatatcc tacgccatct
    25201 aaaatagctg tttttttgtc tgaatcactc ccttgtggta aggtagtcac attataatca
    25261 atctcgtttg ctttcaatcc gttatttaac catggcccat tgatttcaat ggctagttga
    25321 ccagcattca ttaaattgtc catttcggcg ccagaaatcg attctggacc agtcttgtct
    25381 acatggatca ttttttggat cgcttttaac gttttaaggt tctcaggaga cgcaaattca
    25441 gacttagtat aatcatcgtt cactaattta ccgccatttg ctaacatcca gttatataac
    25501 ggtgcagttc catccttatt gaagacaaaa cctgatacat ttttactact atcagttaat
    25561 tttggtgcca tttctgtcaa ttcttcccaa gtagtaggtg gttgttcagt atctaatcca
    25621 gcttgtttaa acaaatcatt attccaatac atatacatgc cttgtacctg catggggaca
    25681 aaatactgtt taccttctac ttcacctaat tcaatagccg tttctttaaa atcagattta
    25741 tctactccat catattccca gaaatcgtct aatggttgta ccgctccgtt ttctacatac
    25801 tgtgcaaaat ctggataatt taatgcaata aagtcaggtg cttttttaga agaaatcgct
    25861 gtaggaagtt tttcattcaa attggcccaa gtcatgatat ccatttcaat tttgatgttt
    25921 ttatcatttg ttttattaaa ttcattaacg atttcttgca atatttcacc atcagatgca
    25981 gtaaacccat tccaaaaagt caaagtcact tctttatcag aatcagattc tgctgaactt
    26041 ccgccaccgc aagctcctaa aatacctgtt gcagctacca acaaagagcc aattaaccat
    26101 tttttacctt tcattttttg ttcctccttc atctgataaa cgacatctga ccacattcca
    26161 agatagcggt ttcaataaac aattgatttt cccatcttca gcttttacat ttgttgaatg
    26221 acttggtttt acttgttcac ttttaggaag attagttgct tttgtatcaa atccactcat
    26281 ttcagaaaaa tcaatcactc cctctaatga taattcagac aagtgaacag ttagattcat
    26341 ttcttcttct ccacggttaa ctgcaaatat gacaagttct ttgtcattta ttacggctat
    26401 actttctaaa tacggcactt cttcaaaatc ttccgtcgca tagtttgctg aacgaatttg
    26461 tgggcttaac acctctcctt gtccatacac agcagcttgc ataaatggat aatagatcgt
    26521 ctgtctccaa gcttctccgt ttgtttctgt cctgattggt gcaataacgt ttaccaattg
    26581 agcaagacaa gcaatcttta ctctatctgc atgtttcaat aaagtgatta atagacagcc
    26641 taatagtaat gcatcttcaa aattatagtt atcttctagt aaaggaggag caatctgcca
    26701 aggcgctgct tgctcatctt gttccgctga atgataccaa atattccatt catcaaagga
    26761 caggttgatc tgcttcttac tatgtttctt cgccttgact gtatcgcaca tcgctgtcac
    26821 ttcagaaatg aaccgatcca tgtctaagga acgtgcaaga taatgagaaa gatcattttt
    26881 ttggttgcca taatattgat gcagagacaa ataatctatt tgatcgtacg cttgatcaag
    26941 aactgttaac tcccaatcac caaatgtagg catttgccga ttcgaacttc cgcataaaac
    27001 cacctcaatt gaatcatcta cgagtttcat cgccttagcc gtttcctctg ctaaccgtcc
    27061 atattcttcc gctgttttat gtccaatctg ccaaggaccg tccatttcat tccctaaaca
    27121 ccatgtctta attccaaaag ggctctcttg accattcgaa cgccttaaat cactccaata
    27181 agttccttta ggaaaattac aatattcgac taaattacga gctgcatcta ctccacgagt
    27241 acccagattg acagccatat tgatttcggc ccccacatgc tgacaccagt tataaaattc
    27301 atgaatccca acttcattcg tttcaattgt ccgccaagct aaatccaacc ttctaggtct
    27361 atttgctatg ggcccaatac tgtcttccca acgaaatcct gacacaaaat ttccaccagg
    27421 atatctaatc atgggaatgt tcaattcttt taccaagtga atgacgtctt ggcgaaatcc
    27481 acttttatta gctgatggat gatctggctg ataaatccct tcatatactg ctcgtcccat
    27541 atgttcaata aatgaactgt acaaccgcgg atcaactttg cctacacgct tgacactact
    27601 aatcaacatt gtggcttcca taactgacct cctttaattt atacatgaat aatacacctc
    27661 gttgaaagcg ttgtcaataa tataaatgtt aaacttactt attttttatc gattttaatt
    27721 aaccaaaata ttaataatga ttaaaacaaa taatttaata taacttttat taacaaatat
    27781 gttaatatat aagaaaaacc ttttaagagg tgatatatat gcaactacaa ataaacgaag
    27841 aatccttacc ggtatatgaa gcattagcta gtaaaacaag gatcaagatc atccagctac
    27901 tttccaaaaa aaagatgaat gtcaaagatt tggcaaaaga acttggagtg agcagtgcta
    27961 tcacgacgat gcacgtgaaa aagctagagg aagcaaacat catcaaaaca gaaaaagtag
    28021 gacaacaaaa aatttcgagc cttcgtgtcg ataaaatcga tattagtttt ccagaaaaaa
    28081 tattcaatgc ttttgatacg aaagaaacat cgatacctat tggtcactat acaaattatg
    28141 caattgaacc aacatgcggt ttagctacga tccatgattt tatcgggaaa gtggatgaac
    28201 cacgctattt tatggatcct cgaaggatgg atgcccgcat tctctggttt accagcggat
    28261 ttgtcgagta tcaggcacct aattttttaa atacagagga tacattggag atgttggaag
    28321 tttctgtgga aatttcttct gaatttcctt tttcaaatga caattggcct tcagatatca
    28381 ctttcagcct aaatggtgtg gagctaggaa catggacgag ccctggagat tttgctgata
    28441 tccgcggtaa atatacgcct gattggtatc cagataatct taatcaatac ggcttgctga
    28501 agacgatccg aattaccaaa cacttgacga atatgaatgg agaacccttg tcaaacatca
    28561 cgatcaacga catccctaaa gagcaagaca cttggcattt gcgaatcgaa gtgaaagacg
    28621 atgccaaaca tgtggggggc tgtactttat ttggtaaagg attcggtaat tacgatcagg
    28681 atatcaaatt aaaagtttac tacagctgat atttttcttt tctattttat aaaacaaaaa
    28741 cagccaatcc ctatgaaggg acggctgttt ttgctttatt gttatttcca gaaatcatcc
    28801 aaaacggaaa ttggcaaatg tcgtttatgt tgtgtcttgt tccaccatgc ttcgattttt
    28861 tcttgtgctt gagcgggaac ttcttttcct tctagataat catcaatttc attatatgtg
    28921 acacctagtg cgacttcatc tgcaatcatt ggtttcccat cttcaagatc tgcagtcgga
    28981 atttttgtgt ataatttttc cggtgcattc aatgcttgaa gcaattgttt cccttgtcgt
    29041 ttgtccaaac ggaataatgg caaaatgtcg gctccaccat caccaaattt cgtgaagaat
    29101 ccagtaatgt tttctgcagc atgatctgtc cctaatacgg ctcctgctct ttctccagct
    29161 acggcatatt gcgtaatcat acgttgacgt gctttgatat ttcctttgtt aaaatcgctg
    29221 attcccacac ctgcttcgct taatacttgt acttgtgcat caacagctgg tttgatattt
    29281 actcgcagac taacatcggg ctggataaag tcaagagcag cttttgcatc ttcttcatct
    29341 gcttgttcac cataaggaag acgaacagcg ataaattgat agttttcatc ttttgtttct
    29401 tcacgcattt cttccatcgt tagttgcgcc aaacggcctg ctaaagtaga atcttggccg
    29461 ccgctgatac ctagtacaaa agttttaaga aacggatatt ttttcaaata tgctttcata
    29521 aagtcaatac ttttacgtat ttcttctttt ggatcaattg ttggtttgac acctaattca
    29581 ttgataattt ctgtctgcag gctcatttat tctcctccta tgcttctgta tgaagttctg
    29641 ctacgctttt tttcattcgt tccatgatag ccattttatg gttccagcat tcagtagaca
    29701 gatcgactgg gtatttttgc ggattcaagt ctcgtttata ctcttcccat agtgaatcta
    29761 gattttcttt tgtatattct ttgatttcgt ttagtgcagg taattcgtaa actctttttc
    29821 cttcgataaa gatatcttgt aaaactgggc gagctgtaaa gtctcgaaca gttttattga
    29881 taaacgtatg cactggatgg aacatgaaga tagcttcttc ttctcgaggg tcttcatccc
    29941 ataatgtcac gtagtctcct tcagatttcc catcaaaatt tcgagtgatg cgccacactt
    30001 gttttttccc tggagtagtt actttttctg cattgcttga taacttgatc gtatccatca
    30061 tttttccttc atcatcttcg atcgatacta gcttgaatac tgcaccaagg gctggctgat
    30121 cataggctgt gatcaatttt gtcccaacac cccatacatc aattttagct ttttgcatct
    30181 ttaaattcaa aatcgttgct tcatccaagt cattcgaagc atagattttt gcttccgtaa
    30241 atccggctgc atctaattgt tcccgaacac gcttagatat ataggccata tctccactgt
    30301 cgatgcgtac gccttggaag ttgattttgt cacctaattc tttcgccacg cgaatcgcac
    30361 ttggcacacc tgatttcaac gtatcataag tgtcgactaa gaacacgcag tctttatggg
    30421 ttttagcgta ggccataaat gcttcataat catttccata agattggact aacgaatgag
    30481 catgggtccc gctagctggt atcccaaata tcttcccagc acgtacattg cttgttgcat
    30541 ctgctccgcc gatatatgcc gctctagttc cccagactgc tgcatccagt tcttgtgccc
    30601 gtctagttcc aaattccaat agtggatctt ctccgatgac agatttgata cgagctgctt
    30661 ttgtcgcgat caacgtttgg aaattgacca tattcaacag tgctgtttct actaattggc
    30721 agtgcgctaa aggcccttcc acttggatca atggttcatt tgcaaaaact agttctcctt
    30781 cacgagcaga acgaactgtt gctttaaatt caaaattttg taaatactcc aaaaaacctt
    30841 caggatagac ttctaatcca cgcaagtaag caatatcgga atcattgaac gtcagattct
    30901 ctaaataatt gactaaccgt tccaatcctg caaaaaccgc gtatccgtgg ttaaaaggca
    30961 tttctcgaaa gtaacattca aaaacagcat gtaaatcagc tcgacctaat tcccagtatg
    31021 tctgcatcat attgatttga tatagatccg tatgcagcgt caaactgtcg tctgggtaag
    31081 tattattcat cttgtattac tcctcttggc aagttttcat ttcctaccca ttataacaaa
    31141 aaaaattaat aggaaaagaa atcttccatt ttttctgaaa aagcacatgt tttacggata
    31201 tttcttgcta aaaatgaact ccatctgcta aaactatcct ttcttattgg tatttaacct
    31261 aaacgagaaa gaatacttat tttattcaat gtatccgaga cacgaagaaa ttagcgtttt
    31321 ctatttttct tacttgataa aataagtaga ttttcctact acaatcgtag ccgttaagat
    31381 accgatgatt gaacaaatat aaccaaatgt ctctacaaca taatcaatgg caaaagtttt
    31441 tgaagcaata atacgtcttt ccttttttgc ttcttatccc caaagaaacg gatataactt
    31501 attatgtatt gaattttttt ggaaaggcga attatgacaa gatatacctt gcgttgaaaa
    31561 aactcgcgga aaaaatatca cacgttttta aaaagataca aagtaagaaa gcagctgtca
    31621 atagaatgga aacaaaaatg gcgagacatc aatcagtctc gccttgtttt ttattgtata
    31681 cattagatat ggagcaggtt accataaaga tgattattag tcgttattaa atagaatcct
    31741 tcgtattttt ttgttctatc actacaatat ctttatttgg acgtttgaat tgtattccgg
    31801 cacgttctag ctcctgtttc actcctacat ggactttgac atcttctcgt gtcattttta
    31861 aaacgtgatg aatcgcatag tcgatttctt gccttgtgat ttttttcttc gtattgatca
    31921 tcacgacttg ctttttatca tatgtgtcta ccaatagcag ttgtgttcga tctaacgaaa
    31981 ataatttgta ataattaata gccgtattcc caaaaaagaa ttccactact ttaggataaa
    32041 atgtcttaat atttaaatta ctattcacag gtgtttcgat tagcttcatc tcattcctct
    32101 tttctatata gtcgatttag atccaatctt aagttatgtt accatctttc ctagcattaa
    32161 ggaatcggtt acaattgaac ctttaaagac aaaaggattt accttttatc acttaatagt
    32221 ataacataat ttcatgagaa aaaaatacaa ttttttcttg accatctcgt tacgtaaagg
    32281 attatgctta catttgtcag gaggaaaaat aatggagtac acaataaaga aaatgtcaga
    32341 gatatcagga gtcagtccga gaaccttgcg cttttatgat gagattggac tgctaaaacc
    32401 agctaggatc aattcttcag gttatcggat ttatggcaaa aaagaagtcg atcgtctgca
    32461 acacatctta ttttatcgaa caatggcatt caagttggat gatatccaag aggtcttaga
    32521 caatccatct tttgaccacc agaaagcttt aatcaagcat cagcaaatgt tgctggaaaa
    32581 acgggcacaa atcgataccc tcttgacaac tgtccaacag acacttgata tgtacgaagg
    32641 aggaagaaaa atgagtgaca aagaaaaatt tgaagggttt aaacaacaaa agttgaaaga
    32701 aaatgaagaa tcctatggac aagagattcg acaaaaatac ggaaaaaaaa aggttacagc
    32761 atccaatgaa aaatggatga atatggataa agaaacgtat gaagaaatgc aaactgttga
    32821 aaagcagatg ttatttgatc tcactcttta cttaaaagaa ccagaagatg aaagcttagc
    32881 agaccgtatc ttccaagctc ataagaagtg gttatcttac agctggcctg aatatcagcc
    32941 aaaagcgcat aaaggactag gaatgatgta cgaagccgat gaacggttca ctgcatatta
    33001 tgacgagcga agtggtcatg gtgcagcaaa agctttaaat gaaattatcc aaaaaaaggc
    33061 gtaatctttt acatgatcca acaaaaaagt acacgtaaaa tgacctaata acaaaacagc
    33121 agacacccaa gttagatttt tatgctaact tttggtgtct gctccattct ttcacaagtt
    33181 ttttcatcct actttaaata tattcatcgt tgaataaacg tataaggtat tttcacttta
    33241 tccttttttt ctgtaagaaa caattgaaat gccatttctc cgcaagcttc cagatggtgg
    33301 tcgatggttg ggaaaccaag aagctcgctg atcagcaaat tatcgcgtcc aatcaccact
    33361 ggtgtcttat cttgcagata gctttgcaag atgcctgctg caaccatatc cgaatttgta
    33421 aagacaccat caactccaag ctctttaaaa aagaagcctg cttgttttcc tttctctgca
    33481 tctatacaat cccaaaaaac aagtgattca tcaaaagatg gaaaaagttg atgacatagc
    33541 tgcaaagtga tttttgagtt ataacttagc ctgccacttc gccccaacgt gatgcccaat
    33601 tgctttatac cttgctgctt caaataagta agaccttctg taattgattg ttctcgatca
    33661 ataaagacac agcttgcctg ttttcccttg atttcttcac aaaaaactag aggtccatat
    33721 ttttgatatg ccaataaatc atctagtgta ttggcacgag acgtaatgat caatccatcg
    33781 aaagctttag ctgcaaattc ttcaagatac cttcgctctt tttctttctg atagcccgtc
    33841 ggtaaaagtg tcactttgta atcataatca aacgctgctt caatgatccc actgatcaac
    33901 tgatcaaaat acggatgatt ggtataaggc agaatcactc caatgctttt cgtttttcca
    33961 taacttaact gtctggcatt tgcattaggg atataatcga cttctttcat gaccgcaagt
    34021 atttctttcc ttttttcctc atctacatat ggatgtccat tgattacacg tgaaatagta
    34081 gaaactgaat aacctgtgag ctttgcgata tctcgaatgt ttgccactgc tgcttcgcca
    34141 cctatacaaa aagattttct tgtaaatagt ctaccacagc tggaccgcat tcaaaaaact
    34201 gatacatttt tccttcatgc gaaaaactga tccgctgccc gccccaatat atatcttttg
    34261 tcaaattttc gatttcattc attggtattt cttttactat attcctattc aacccttgaa
    34321 aactgtacaa cgttaatacg gatcccttga actcgacaaa cgcataaaaa taattgttcg
    34381 ttgtgccatc tgttatctct acataagtat tcattgcaag ccctcctttt gatactttga
    34441 gtatagtgta tgaaacgcgt ctcatagcaa ggagtttgtt ttgccaaaga aaagttttat
    34501 tcctctttca ttattaccaa agtctcttat tttaaattga atac
//
LOCUS       DAFGZE010000026        34537 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.026, whole genome shotgun sequence.
ACCESSION   DAFGZE010000026 DAFGZE010000000
VERSION     DAFGZE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.026"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            212..604
                     /locus_tag="LTU54_001311"
     CDS             212..604
                     /locus_tag="LTU54_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL8296034.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="LTU54_001312"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="LTU54_001312"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HBL8296035.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="LTU54_001313"
     CDS             3165..4775
                     /locus_tag="LTU54_001313"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HBL8296036.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="LTU54_001314"
     CDS             5067..5936
                     /locus_tag="LTU54_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HBL8296037.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="LTU54_001315"
     CDS             6101..7372
                     /locus_tag="LTU54_001315"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL8296038.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="LTU54_001316"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="LTU54_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBL8296039.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="LTU54_001317"
     CDS             8763..9026
                     /locus_tag="LTU54_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HBL8296040.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="LTU54_001318"
     CDS             9471..9920
                     /locus_tag="LTU54_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8296041.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="LTU54_001319"
     CDS             9977..11395
                     /locus_tag="LTU54_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8296042.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="LTU54_001320"
     CDS             11395..12255
                     /locus_tag="LTU54_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBL8296043.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="LTU54_001321"
     CDS             12429..13202
                     /locus_tag="LTU54_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8296044.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="LTU54_001322"
     CDS             13349..13714
                     /locus_tag="LTU54_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HBL8296045.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="LTU54_001323"
     CDS             complement(13862..15070)
                     /locus_tag="LTU54_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8296046.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="LTU54_001324"
     CDS             complement(15089..15367)
                     /locus_tag="LTU54_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296047.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="LTU54_001325"
     CDS             15521..16216
                     /locus_tag="LTU54_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL8296048.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="LTU54_001326"
     CDS             complement(16658..17455)
                     /locus_tag="LTU54_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBL8296049.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="LTU54_001327"
     CDS             complement(17452..18636)
                     /locus_tag="LTU54_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL8296050.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="LTU54_001328"
     CDS             complement(18661..19908)
                     /locus_tag="LTU54_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBL8296051.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="LTU54_001329"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="LTU54_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBL8296052.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="LTU54_001330"
     CDS             21888..22202
                     /locus_tag="LTU54_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBL8296053.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="LTU54_001331"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="LTU54_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBL8296054.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="LTU54_001332"
     CDS             22887..23531
                     /locus_tag="LTU54_001332"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBL8296055.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="LTU54_001333"
     CDS             23546..23875
                     /locus_tag="LTU54_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HBL8296056.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="LTU54_001334"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="LTU54_001334"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBL8296057.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="LTU54_001335"
     CDS             24863..25687
                     /locus_tag="LTU54_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBL8296058.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="LTU54_001336"
     CDS             25680..26027
                     /locus_tag="LTU54_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HBL8296059.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="LTU54_001337"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="LTU54_001337"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBL8296060.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="LTU54_001338"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="LTU54_001338"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBL8296061.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LTU54_001339"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="LTU54_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBL8296062.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LTU54_001340"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="LTU54_001340"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBL8296063.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="LTU54_001341"
     CDS             31657..32451
                     /locus_tag="LTU54_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296064.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="LTU54_001342"
     CDS             32441..34483
                     /locus_tag="LTU54_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296065.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT        11106 a         6122 c         7250 g        10059 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctacaattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatt
//
LOCUS       DAFGZE010000027        33675 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.027, whole genome shotgun sequence.
ACCESSION   DAFGZE010000027 DAFGZE010000000
VERSION     DAFGZE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.027"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            103..273
                     /locus_tag="LTU54_001343"
     CDS             103..273
                     /locus_tag="LTU54_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296066.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            493..1200
                     /locus_tag="LTU54_001344"
     CDS             493..1200
                     /locus_tag="LTU54_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8296067.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAFLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="LTU54_001345"
     CDS             complement(1259..1903)
                     /locus_tag="LTU54_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBL8296068.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="LTU54_001346"
     CDS             2030..2317
                     /locus_tag="LTU54_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296069.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="LTU54_001347"
     CDS             2425..2745
                     /locus_tag="LTU54_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296070.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="LTU54_001348"
     CDS             2826..4139
                     /locus_tag="LTU54_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL8296071.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="LTU54_001349"
     CDS             4136..4855
                     /locus_tag="LTU54_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBL8296072.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="LTU54_001350"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="LTU54_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBL8296073.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="LTU54_001351"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="LTU54_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBL8296074.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="LTU54_001352"
     CDS             join(8416..8487,8489..9517)
                     /gene="prfB"
                     /locus_tag="LTU54_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBL8296075.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="LTU54_001353"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="LTU54_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBL8296076.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="LTU54_001354"
     CDS             10292..11185
                     /locus_tag="LTU54_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296077.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="LTU54_001355"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="LTU54_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL8296078.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="LTU54_001356"
     CDS             13122..13979
                     /locus_tag="LTU54_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBL8296079.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="LTU54_001357"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="LTU54_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBL8296080.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="LTU54_001358"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="LTU54_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBL8296081.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="LTU54_001359"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="LTU54_001359"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL8296082.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="LTU54_001360"
     CDS             16735..17493
                     /locus_tag="LTU54_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBL8296083.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="LTU54_001361"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="LTU54_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBL8296084.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="LTU54_001362"
     CDS             18406..19437
                     /locus_tag="LTU54_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL8296085.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="LTU54_001363"
     CDS             19434..20201
                     /locus_tag="LTU54_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HBL8296086.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="LTU54_001364"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="LTU54_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HBL8296087.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22166..22486
                     /locus_tag="LTU54_001365"
     CDS             22166..22486
                     /locus_tag="LTU54_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HBL8296088.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            22486..22836
                     /locus_tag="LTU54_001366"
     CDS             22486..22836
                     /locus_tag="LTU54_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL8296089.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            23003..23941
                     /locus_tag="LTU54_001367"
     CDS             23003..23941
                     /locus_tag="LTU54_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBL8296090.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            23956..24786
                     /locus_tag="LTU54_001368"
     CDS             23956..24786
                     /locus_tag="LTU54_001368"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBL8296091.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            24830..25855
                     /locus_tag="LTU54_001369"
     CDS             24830..25855
                     /locus_tag="LTU54_001369"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL8296092.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            25870..26808
                     /gene="galU"
                     /locus_tag="LTU54_001370"
     CDS             25870..26808
                     /gene="galU"
                     /locus_tag="LTU54_001370"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL8296093.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            26933..27373
                     /locus_tag="LTU54_001371"
     CDS             26933..27373
                     /locus_tag="LTU54_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBL8296094.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            27376..27978
                     /locus_tag="LTU54_001372"
     CDS             27376..27978
                     /locus_tag="LTU54_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBL8296095.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQGLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(28041..29144)
                     /locus_tag="LTU54_001373"
     CDS             complement(28041..29144)
                     /locus_tag="LTU54_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBL8296096.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            29354..30355
                     /gene="ccpA"
                     /locus_tag="LTU54_001374"
     CDS             29354..30355
                     /gene="ccpA"
                     /locus_tag="LTU54_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HBL8296097.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            30710..31105
                     /locus_tag="LTU54_001375"
     CDS             30710..31105
                     /locus_tag="LTU54_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296098.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREMQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGSYRIVILMTNKQDQSIYFSYSFWKERNEIGSY
                     GIVDEKKQKEGNTLNKVKVTYSNGNEESI"
     gene            31105..31356
                     /locus_tag="LTU54_001376"
     CDS             31105..31356
                     /locus_tag="LTU54_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296099.1"
                     /translation="MITDKQYSELSDAVYWLDPKHRDYVPEMQENLSFKINGTMYKIL
                     KIKNSFDGMQAMAVAPIVHSKLEKNFKNKKIPANFRVLK"
     gene            31399..31794
                     /locus_tag="LTU54_001377"
     CDS             31399..31794
                     /locus_tag="LTU54_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296100.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGSYGALVTMTNNEGKSVYFDYSFWKERNELGGF
                     GIENRAVQKTGVTIKRVKVIFSNGQEELV"
     gene            31791..33047
                     /locus_tag="LTU54_001378"
     CDS             31791..33047
                     /locus_tag="LTU54_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HBL8296101.1"
                     /translation="MTINDKHYNDISEKVYWLDPKYPRYNEGYKKNSVKEFAGMEFQI
                     LQIKDSLDGMQAMSVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAFLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSAKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            33059..33382
                     /locus_tag="LTU54_001379"
     CDS             33059..33382
                     /locus_tag="LTU54_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296102.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            33375..33641
                     /locus_tag="LTU54_001380"
     CDS             33375..33641
                     /locus_tag="LTU54_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296103.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNYQ
                     MTAEEAAQEFSNRHVHLKGSVKGQFAEKLTELFQRQEKKLKQFR"
BASE COUNT        11283 a         5791 c         7245 g         9356 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcatttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gatagtctat ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga
    22021 gtcacaagga ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac
    22081 cgagaatttt gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt
    22141 taaaatagta gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ctaataaaaa
    22201 tgttgttttg acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt
    22261 tatccgtgtg ctttatgtat tcctgagctt agttgctttt ggaagtccaa ttattttata
    22321 cattttatta gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa
    22381 caatgcatat tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg
    22441 caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa
    22501 cgtattttag ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc
    22561 catgtgagca gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg
    22621 tttgtcaaac cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg
    22681 tttacgtttg ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa
    22741 cattttagtt tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc
    22801 aatgtaattg tcagcgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg
    22861 cgcagttttt ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa
    22921 acgttttttt taattggtag tacttttggc tagacaatga taaaatgata gagaacactc
    22981 taatagaaat gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa
    23041 attatctttg gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg
    23101 ggacatctca cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg
    23161 tttacagcta tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg
    23221 tttgatcatc atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg
    23281 attaccagct ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg
    23341 ttctcccatc tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct
    23401 tgcccctcga acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat
    23461 tcaaggtgac agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggcca
    23521 tcgattgatc gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg
    23581 agaaccgcct aaaatcctgg aacatctgat tgaaatccga ggaatcggga tcattgatgt
    23641 aatgaatctt tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta
    23701 tctggaagca tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt
    23761 tgaaatcgcg gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt
    23821 tgcaatcatc attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc
    23881 aacgaaaaca tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta
    23941 gcaaggagtg acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt
    24001 cctatctact ggtatgcatt gatcatcgta tcaggtatca ttatcgctat gtggcttagc
    24061 agcagagagg ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg
    24121 ggattgccta tctccattat tggcgctcgc ttatattatg ttttatttga tcttccccag
    24181 tatatagctg atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc
    24241 ggactgattg caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg
    24301 tggacttttt tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg
    24361 ggaaatttca tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt
    24421 ctgcatttac cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca
    24481 acatttcttt atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga
    24541 aagaaaccac accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt
    24601 ttcgggcgtt tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc
    24661 cgtatttctc aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg
    24721 cgcagaaaaa aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt
    24781 atctgatttt tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag
    24841 agtagcggtt ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa
    24901 cggacatgac gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca
    24961 acacacgaat caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac
    25021 caatatggaa gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc
    25081 aatacgttct gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca
    25141 tgcaagtaaa ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga
    25201 aatcccagca gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga
    25261 agtagcaaaa catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa
    25321 atatgtacaa gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat
    25381 cggtgtagag atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca
    25441 cggcttaggg tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat
    25501 cagccgttta ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggaat
    25561 aggcgatcta gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt
    25621 gttaggacaa gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg
    25681 tgtgaatact acccaagcag cgatggaact agcccaaatg atggaaatcg aaatgcccat
    25741 cactcaaaca atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga
    25801 aatcatgtta cgtgatggaa aaaaagaaaa tgaattctca ttaacaattt tttgaaggag
    25861 cgaatgtata tgaaagttaa aaaagcagtt attccagcag caggattagg aacaagattt
    25921 ttaccagcaa caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc
    25981 cagttcatcg ttgaagaagc gttgaaatca ggtatcgaag acatcttgat cgttacagga
    26041 aaagctaaac gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta
    26101 agtgaaaaag gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg
    26161 catttcatcc gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc
    26221 tttattggaa atgaaccatt tgtcgttatg ctaggcgatg atataatgga agacgaagta
    26281 ccattgacac agcaattgat cgatgactat gagcgtacac acgcttctac gattgccgtt
    26341 atgcaagttc ctcacgatga aacttcaaaa tacggaatca tcaacccagg ggaagtactg
    26401 gaagatgggt tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca
    26461 agtgatttag ctattattgg gcgttactta ttgactccag aaatctttga tgtattatca
    26521 gaacaacaac caggtgctgg aaatgagatc caactaactg acgcaatcga tacgttgaat
    26581 aaaacacaac gcgtatttgc ccgtaaattc acaggtaaac gttatgacgt tggtgacaaa
    26641 tttggtttta tgaaaacaag tattgaatat ggtctgacac atccagaagt aggggcacct
    26701 ttgcgtgaat acatcatgtc tttaggtaaa acattagaag cagaaggaag caagatcaat
    26761 cataatccaa aaggaataac agaaatcaaa gaagaaaaga aaaaataatc tttttttcta
    26821 acgagcttca gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa
    26881 aacacgaaaa tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg
    26941 aggagaaatt gcaggattga tcgcagcaat cgcctttgcg atacttgtcg ttttcatcgt
    27001 tcgcgtattg ttgcaagttt ccaaaacttt agaaaaagta gaccagactg tcgcagaagc
    27061 aaatacgaca attgagatcg tcacacaaga cgtagatctt ttatctagac aagtagaagg
    27121 cttgctagtc aaaagtaatg agctgctctt ggatatcaat ggaaaagtcg caacgatcga
    27181 tccattgttc acagcagttg ctgatttaag tgaaagtgta tcggaactta atcattctag
    27241 taaaaacatc gtgacgaaag tcggcggaat cggaaaaacg acagctaaag cgactgttgc
    27301 cggaaaagtc ggtggaacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac
    27361 aggaggaaaa taagaatggc taaaaaaggt ggatttttat taggtgcagt agttggaggt
    27421 acagccgcag caatcgccgc attattgtta gcacccaaat ctggtaaaga acttcgggat
    27481 gaattggctg ctcaagcaga tgatttgatg gacatggctt cagattacac agatatggca
    27541 aaagaaaaat catctgagtt aacaggtatt gcaaaagaaa aagcgcaagg tctttctcaa
    27601 caagcagggg atctagcagg caatgttaag cagaaggtaa gtgaatcagc agaggaagca
    27661 ggaagtgaat cagatgatat cttgttaaac ctcaaagaac aatcagctga tttatctaat
    27721 cgtttcaaaa aaacagcaga agatctgaaa aagccagcag acgaattagg tagtattgct
    27781 aaagatactt cagaagatat catgatcgat gtaaaagatg ctgcagataa agcaggagag
    27841 acggtcaatg aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgttg
    27901 aaaatggcaa aagaagatgc aaaagaagct aaaaaagaaa tgaaagatac aatgacagat
    27961 tcgactacca atcactaaga ttactcattt ctagaataaa aaaggtgaga gcggaccagc
    28021 tctcaccttt tttattttca ttactctaaa acaagtaatt cttttggtgt agtcgtgaat
    28081 ggcacacaac catcacttgt cacatacaca caatcttcga tacgtacccc tactttttca
    28141 ggaatataga ttcctggttc aagagagaag cacatccctt cttcaatcac taagtcgttt
    28201 ccttctacaa gagaagggaa ttcgtggacg gttgttccga ttccgtgacc taaacggtga
    28261 ttgaaatatt cgccgtagcc atagctgttg atcacgtttc gtgcgatttg atcaagttca
    28321 cctgctgtca cacctgggcg aactgcctct tgtgcggcaa gctgtgcttc aagagtgatg
    28381 ttatagattt tttcttggaa ctcagttggt ttttgataag caactgtacg agtagcatca
    28441 ctgcaatagc cattccaaat aacgccaaga tcgaataaca ctagttcatt tggtgaaacc
    28501 ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg ctcctgcaag gactaatgtg
    28561 tcgaaagaca tttgtgaaac accttgtttt tttaattggt attcgatttc tgcgataatt
    28621 gcttgttcag ttacgccttc ttttactgtt ttgaagccaa tttcaaaggc aacatcggcc
    28681 catttaccag cttcgatcag tttgtctttt tcttctaccg ttttgatcag ttgcagacgc
    28741 tggataatag aagttagatc ggtgtaagaa tctgtacctg tcaagatatc agagatagca
    28801 tcgaagcgat cgacagtcag agcattcttt tcaataccta cattaccagg ttgcccatag
    28861 cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt cgctgtctaa gtagccacgt
    28921 acatcgtacg cccagctgct gttttgagca tcttcaactt ccagagcagg agtgaaaagg
    28981 aaaggatctt gttccagagg gataaaaaga gctagtacac gctcgtgagg atcactttca
    29041 tatccggaaa agtaagcgat gtggccaggg tcgctgatat aagccaattc tacttggtgt
    29101 tgtgccatcc attgacgtag ttcattgatt tttacgttat tcatttggca acctactttc
    29161 taaattaatt ttgtattcaa taattgtacc actgattgat taaaagacca acgttcttaa
    29221 aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa acggttgcaa ttgttgagat
    29281 tatttgatat cctattttag aaatgtaaga cgtattttca gaaaacatat aaaatatgaa
    29341 taggagaaca caaatggaaa agcaaacaat cactatctat gatgtagcga gagaggcaaa
    29401 tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat ccaaacgtca aaccagcaac
    29461 aagaaaaaaa gtcttggaag ttatcgatcg cttggattat cgtccaaatg cagtcgcacg
    29521 tggcttggca agtaagaaaa caacaacagt tggggtaatc attcctgatg tcagcaatat
    29581 gttctttgcc tctttagcac gtggtatcga cgatgtggca acaatgtaca aatacaatat
    29641 catcttagca aactcagatg gcaacgatca aaaagaagtc aatgtattga ataatttatt
    29701 ggctaagcaa gtggacggcg tgatctttat gggtcaccat atcacagacg aaatccgtgg
    29761 agaattttca cgttcaaaaa cgcctgttgt actagctggt tccattgatc cagatgaaca
    29821 agtaggtagc gtaaacattg attatgtgtc agcaacaaaa gatgcagtaa ataaattagc
    29881 aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg ctgattgatc ctatcaatgg
    29941 tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa aacaatctac catatagcga
    30001 aggattgatt tttgaggcac aatacaactt taaagatggt ctgtctctag ttgaccggat
    30061 ccataatagc ggtgcaacag ctgtctatgt ttcagacgat gaattagcca ttggtatctt
    30121 ggatggttta ttagaccgcg gcgtaaaagt accagaagaa ttcgaaatca tcacaagcaa
    30181 caattcttta ctgacagaag tagctcgtcc tcgtttgaca agtatcacac aaccattata
    30241 cgatttagga gcggtatcca tgcgcctatt gacgaaaatg atgaataaag aagaagtcga
    30301 agaaaaaacg attatcttgc cttatggaat cgaagaaaaa ggatcaacta aataataaaa
    30361 gatcaatcat ttaaaggttg ttccaaaagt ggtcaacttc tggaacaacg tttatacgag
    30421 aaagggggtt atgacaaaat tttgtcataa cccctttttt tttgtgactt taaaaaaaga
    30481 gaaaatggcg gaaacaaacc ttacgtccat cgtatacgac acaatctgtg cgtagatgct
    30541 acattttgtt cactactatc aaaattactt taaaatgaaa acgtgataaa aaagaaagta
    30601 gaaaagtaga taagtaactc taaaaaatcc aaaaaaatcc aggctcgatt gcgtacttat
    30661 cttaaaggag aaacaatcca tcatatataa cccgaaagga gacttctatt tgcctaagaa
    30721 aagacgaaag accatagtca tcattttgac cattcttact ataggaatcg gagggaaatt
    30781 ttacatggac aaaagagaaa tgcaaagaca gaaagattta ctcgcagtag aaaaacagtc
    30841 cgtcaaagtt ctgaaaaata cctttgcgga tatcaaggaa gtgaagattg aaaaatctgc
    30901 tcgaaatgaa atgacgggtt cataccggat agttattcta atgactaata aacaagatca
    30961 atcgatttat ttttcttata gtttttggaa agaaagaaat gagataggaa gttacggaat
    31021 agttgatgaa aaaaagcaaa aagaaggaaa tacgctcaat aaagttaaag taacttactc
    31081 taatggaaat gaggaaagta tttaatgatt actgacaaac aatatagtga attaagtgat
    31141 gctgtttact ggctagatcc taaacataga gactatgtac ctgaaatgca agaaaattta
    31201 agttttaaaa ttaacggcac tatgtataaa atcctaaaaa tcaaaaactc cttcgacggc
    31261 atgcaggcga tggctgtagc gccaatcgtc catagcaaat tggagaaaaa tttcaaaaat
    31321 aaaaaaattc ctgcaaactt tcgcgtactt aaataatgaa ggtgtcaata cttacataaa
    31381 aagaaaggag gcacgttctt gtcaaggaaa ataagaaaac tcagcttagc cattttatta
    31441 atcgtcatat tagggatcgg agggaaagtc tatatggaca aaagagaagc acaaaggcag
    31501 aaagatttac tcgcagtaga aaaacagtca gtcaaagttc tgaaaaatac ctttgcggat
    31561 atcagggaag tgaaggtaga agaatttaaa aaaaaccctg ttaccggaag ttatggtgca
    31621 cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt ttgattatag tttttggaag
    31681 gagagaaatg aacttggtgg ttttggcatt gagaacagag cggtacaaaa gacaggagtc
    31741 actataaaaa gagtaaaagt aattttttct aacggacagg aggagttagt atgacaataa
    31801 acgataagca ttacaatgat attagtgaga aggtttattg gctagatccc aaatatcccc
    31861 gctacaatga gggatacaaa aagaactctg ttaaagaatt tgctggtatg gaatttcaaa
    31921 tcctacaaat caaagactca ttggacggca tgcaggcgat gtctgtagcg ccaatcaaga
    31981 acggtaaagt ggatctttct caagtagtga ttgcttttgc aggaacgaac ttaagtgatt
    32041 ggaaagattt ggaaacggat gcaagaagta tcatgaatct ctacgggaat agaccaggag
    32101 caagtttttc tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag
    32161 cggggcaaat caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag
    32221 ctgctatttc tactaccggc cattcattag gtggattctt agcattatat gtagcagcag
    32281 agaaacaatg gaaaaatgtc ggcttcaacg ggccggaccc gtatgatctc ttatcaccag
    32341 aagcaaaaaa atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg
    32401 attttatcgg taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa
    32461 cagcattctt gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg
    32521 gaaagctgat tattcctgaa aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa
    32581 accaagcgac ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac
    32641 tacgattaag tggcggccgg ctgacagtta atgaacaaat ttacttagat gacacacaag
    32701 ctaggttagt agtggaaaag ttatcggcaa aaatgaaaag cgctatggaa gaagttatcc
    32761 gcatcaatca aagaggaatt tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag
    32821 cacgtgttgt agccccttcg ttgactgaac aggaaatcat agaagcatta gctagtgtgg
    32881 gcgccacaaa agaaaactta gtcgattctg cgaaagaagt ctatctgcta cgaatcaata
    32941 aggcaagacg tatgggggag gcctttgata cattggcgaa ggagatccaa gacaaaatca
    33001 atgaaatcgt ggcacgagat cgagaattag cacagcaatt caattaaagg agaagtagat
    33061 gaaactagaa gaattggaaa gagattatcg aagtgaacga agacaattag aagctcaaga
    33121 agatgaattg atccacttta agaatcaagg attacgattg atggagcaag aggagcagcg
    33181 actgcatcat gtattaaaga acttagcttt agataatgag cctctgtttc aagcaagacg
    33241 aagttattcg aattatcagg agcattatca acaagcccta caagtagaac gacgaaaact
    33301 ctatcagcag caagatgaac tagaacgttc ctatcgtcaa aaaatcagac aaataactat
    33361 ggagaaatca tcttatgaat gaatttttaa tgcaattact aaaagaatta gagaaaaatc
    33421 tacatcgatc aaatgtacgt tcagttggta tggggatatc tcagcaaatt tgggcagaaa
    33481 aacgattgac agatcaaaac taccaaatga ctgcagaaga agctgctcaa gaattttcta
    33541 accgacatgt acacttgaaa ggatcagtaa aagggcaatt tgcagaaaaa ctaactgaat
    33601 tattccaaag acaagaaaag aaattaaaac agtttcgtta gtaaagttat atagaaaaat
    33661 tttgtgaaaa tggtt
//
LOCUS       DAFGZE010000028        33187 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.028, whole genome shotgun sequence.
ACCESSION   DAFGZE010000028 DAFGZE010000000
VERSION     DAFGZE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.028"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            131..778
                     /locus_tag="LTU54_001381"
     CDS             131..778
                     /locus_tag="LTU54_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL8296104.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    907..1123
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1171..3816
                     /locus_tag="LTU54_001382"
     CDS             1171..3816
                     /locus_tag="LTU54_001382"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBL8296105.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4126..5439
                     /locus_tag="LTU54_001383"
     CDS             4126..5439
                     /locus_tag="LTU54_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBL8296106.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5429..6082
                     /locus_tag="LTU54_001384"
     CDS             5429..6082
                     /locus_tag="LTU54_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8296107.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6137..6808
                     /gene="radC"
                     /locus_tag="LTU54_001385"
     CDS             6137..6808
                     /gene="radC"
                     /locus_tag="LTU54_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBL8296108.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGIYKLVHINYIRKFTVILPSGNTIMLSIIERHNAFFSS
                     SEDSL"
     gene            complement(6703..8055)
                     /locus_tag="LTU54_001386"
     CDS             complement(6703..8055)
                     /locus_tag="LTU54_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8296109.1"
                     /translation="MKRVALYMRVSTEQQAKHGDSLREQKETLYEYIEQHKDLKVVNE
                     YVDGGISGQKINRDEFQKLLQDVKENKIDLILFTKLDRWFRNLRHYLNTQEILEKHNV
                     SWNAVSQQYYDTTTAYGRTFIAQVMSFAELEAQIDSERIKAVMANKIAQGEVVSGKTP
                     LGYSIENKKLVINDDAPIVIDIFNYFLSSGSLRKTVYYLGSQYGIVRDYQSVKNMLTN
                     KKYIGELRNNKNYCPPIIDKKLFYAVQKALPKNLKTNAKRDYIFKGLLKCSDCQGSVA
                     GQTIKARYKKKDGTESIYERTCYRCVKRRNNKLRCTNKRAFYEKNLERYIFEATKQKF
                     EQIQINYSKKQPKIIKKKNSKKNIENKLDRLKKAYLNEVIDLEEYKKDREALMKELNE
                     IEVEPAKIDIKNVEFILSKEFDEIYKESSEEEKNALWRSIIDNIIVFPDGNITVNFLI
                     "
     gene            complement(8121..9410)
                     /locus_tag="LTU54_001387"
     CDS             complement(8121..9410)
                     /locus_tag="LTU54_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL8296110.1"
                     /translation="MGVLLFLVGIVLIIMGSSKLSEAKKLKDKTSALESKVKEYSQEL
                     NARETFISEKEKNISKLNKNLSTLENKIDILNAESQRLDDEINQKYDTSLVTIYKSVI
                     IPNIDETSSSEIKNKLSLLKIKEKDFILDGAINIPSYLNNREANNIKKKMLIPLESEI
                     TGLLNKLTISNVDSTREKIIRTFDKINKLFKNDEAEFKKELLELKLQELELNYSYIVK
                     VNDEKEQQKAIKEQMIEEEKVRREIEREKKRIDKEERQFNSEITKLMAYMQKASADVE
                     KELYADKIKELEEKLRELETVKEDVLQRELNTRAGYVYVISNIGSFGEDVYKIGMTRR
                     LEPMDRIKELSSASVPFEFDVHAMIFSEDAPTLENQLHSHFRKNEVNKVNQRKEFFKV
                     SLDEIEKVVLENYNGTVSFTKLAQAEQYRRSLELTDV"
     gene            complement(9497..10201)
                     /locus_tag="LTU54_001388"
     CDS             complement(9497..10201)
                     /locus_tag="LTU54_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBL8296111.1"
                     /translation="MFAENLKFLREKYNIDQQELAEKLGRKSSSSISEWEKGKYTPKL
                     KTLNEIAKIFNVDIDDLMNIDLSISNQIYNILPIYNQLNDENKVATYEFASNRLEEQK
                     QENVLDFPKKEKIPTVHNSASAANPTELTYGDTVVEEEEFERVPSAADFAVPIIGDSM
                     EPVIRNGQFVFVKEQPDVEDGEIAIVELGGDGVTCKEIYKDYENQTIILRSINDLYED
                     RIVSPEQIRIIGKVVF"
     gene            10401..10667
                     /locus_tag="LTU54_001389"
     CDS             10401..10667
                     /locus_tag="LTU54_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8296112.1"
                     /translation="MDTAKKEKYTLRQLRALKGYSKEELSRKSKVTSRTIFIYENDVD
                     KMRNGKYATLEKIAKALGVRVSDIFLDPDSEKPKSITGETKQVS"
     gene            10680..10838
                     /locus_tag="LTU54_001390"
     CDS             10680..10838
                     /locus_tag="LTU54_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296113.1"
                     /translation="MEEKNIKVPTLEEYTKIVVETDEQNPKTIAVITANDIESCEGFR
                     VRMTPRYD"
     gene            complement(10835..11065)
                     /locus_tag="LTU54_001391"
     CDS             complement(10835..11065)
                     /locus_tag="LTU54_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL8296114.1"
                     /translation="MGKNQWVSPRNGKWAVRGEGNSEDTKLFDNKSDAVEYGKKISKK
                     QQSEFIVQKKNGQIQSKDSFGNDPIPPRDKEH"
     gene            11137..11292
                     /locus_tag="LTU54_001392"
     CDS             11137..11292
                     /locus_tag="LTU54_001392"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296115.1"
                     /translation="MSNKPKITLNGTPNLQRLADEMARIRSRHWGVTVVATIIEQKEK
                     PTQPASK"
     gene            11449..11604
                     /locus_tag="LTU54_001393"
     CDS             11449..11604
                     /locus_tag="LTU54_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296116.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGEKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(11601..11825)
                     /locus_tag="LTU54_001394"
     CDS             complement(11601..11825)
                     /locus_tag="LTU54_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL8296117.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            12092..12427
                     /locus_tag="LTU54_001395"
     CDS             12092..12427
                     /locus_tag="LTU54_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296118.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            12660..13601
                     /locus_tag="LTU54_001396"
     CDS             12660..13601
                     /locus_tag="LTU54_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBL8296119.1"
                     /translation="MSKSTLEMSHQEWLEDRKRGIGGSDVATVLGLNKYKSPYQLWLE
                     KTGQIELKDLESEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPVIDGSGATADFLKEKYADVEENQTA
                     LPSRFDELIEQKRELKRTKKEIESSIRQVDNEIISELGKREASIGITQRNIISWKLVR
                     TRRMNTKKLAEKYPDVANDEEIYNVTESRRLTEKEIK"
     gene            13603..14493
                     /locus_tag="LTU54_001397"
     CDS             13603..14493
                     /locus_tag="LTU54_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HBL8296120.1"
                     /translation="MATNESLKNQLAEKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            14536..15336
                     /locus_tag="LTU54_001398"
     CDS             14536..15336
                     /locus_tag="LTU54_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBL8296121.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWSLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            15351..16202
                     /locus_tag="LTU54_001399"
     CDS             15351..16202
                     /locus_tag="LTU54_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8296122.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            16199..16516
                     /locus_tag="LTU54_001400"
     CDS             16199..16516
                     /locus_tag="LTU54_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296123.1"
                     /translation="MNLTKQEKRICRQIMLQIIGDRDMDELSQQESRMVRKISKLIGA
                     DTMDRSKPLPKLNFESFTFEKYSHLREVGYSVNAIRKALGVGSSVWAKWRKENVNEVN
                     QND"
     gene            16509..16691
                     /locus_tag="LTU54_001401"
     CDS             16509..16691
                     /locus_tag="LTU54_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296124.1"
                     /translation="MIKLIGKETLKVYYVAETKAEVSRWLLKMFNKTRANASVSQKAS
                     VSETGMPEAMWISRRN"
     gene            16693..17163
                     /locus_tag="LTU54_001402"
     CDS             16693..17163
                     /locus_tag="LTU54_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296125.1"
                     /translation="MAMIPKFRAWDREKDRMIYPSTTGVCFEMNDDGISVLDIDVDYP
                     NDYGFPKIASILMQSTTLKDKNGVEIFEGDIALITHKSTNYADTYWHSYVEVFRAKNG
                     AYRIRGEHIYETELYSNRKQLTVAGNVYNSPELLKRDRFMTTTENPELLKEEQR"
     gene            17160..17702
                     /locus_tag="LTU54_001403"
     CDS             17160..17702
                     /locus_tag="LTU54_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HBL8296126.1"
                     /translation="MNKQELIDKYTAEISRLRPYCPNRHLISDQLKYGLYKEILEDLK
                     QLDEPQKPAVPKFVAEWIEYAKKKGDSLAISFKPWNLYGVEYSKTDRWIEDNQGTFAR
                     AWLIGYEVEKEPLYYVKLPVVYFNHWDLETYLMKDDRGNITIADNNDFDDMKFTESEI
                     KAIDERYWPFAVPVEEVAEG"
     gene            17699..18082
                     /locus_tag="LTU54_001404"
     CDS             17699..18082
                     /locus_tag="LTU54_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296127.1"
                     /translation="MSNLKVGDIVFYKGDEGIIREVMTDGKENFYRVDVSRKSILYLY
                     EDELDLGTQKLNDNQKMVFDYLKSNVEQGGKSVMYSIFLLGDWDSRIGAARNVDIAYC
                     GLNGKQEAEVLSAFAQWGLEQEEAE"
     gene            18079..18309
                     /locus_tag="LTU54_001405"
     CDS             18079..18309
                     /locus_tag="LTU54_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296128.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSIDDTFRLGS"
     gene            18316..18519
                     /locus_tag="LTU54_001406"
     CDS             18316..18519
                     /locus_tag="LTU54_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HBL8296129.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            18516..18812
                     /locus_tag="LTU54_001407"
     CDS             18516..18812
                     /locus_tag="LTU54_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296130.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDIELINDK"
     gene            18889..19302
                     /locus_tag="LTU54_001408"
     CDS             18889..19302
                     /locus_tag="LTU54_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HBL8296131.1"
                     /translation="MQLLREVAFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            19315..19701
                     /locus_tag="LTU54_001409"
     CDS             19315..19701
                     /locus_tag="LTU54_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296132.1"
                     /translation="MWKDYVSLKELKKDLIFKKIVEWSESELILEDGTKMEVVCSESD
                     CCAWAEGEFKNVKLDAVITDIKIFDKGNRLYNGDGHSSYAEVVVYHNRNEIAKAECTA
                     NDGNGGYYYSVCALKVKEKLCVVTDA"
     gene            20193..20265
                     /locus_tag="LTU54_001410"
     tRNA            20193..20265
                     /locus_tag="LTU54_001410"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20226..20228,aa:Lys,seq:ttt)
     gene            20651..20911
                     /locus_tag="LTU54_001411"
     CDS             20651..20911
                     /locus_tag="LTU54_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296133.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSVFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            20954..21127
                     /locus_tag="LTU54_001412"
     CDS             20954..21127
                     /locus_tag="LTU54_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296134.1"
                     /translation="MKQYTAKDFEEMKRLKKDYEEVDMELTVGVIQRRLRVGLETAKA
                     IYNDLNAIEEKNG"
     gene            21516..22349
                     /locus_tag="LTU54_001413"
     CDS             21516..22349
                     /locus_tag="LTU54_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HBL8296135.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFETLSDEEKDIYSSLNDNPSFVLSEEIRLLKIRQFRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKNPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            22342..23757
                     /locus_tag="LTU54_001414"
     CDS             22342..23757
                     /locus_tag="LTU54_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HBL8296136.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            23769..25298
                     /locus_tag="LTU54_001415"
     CDS             23769..25298
                     /locus_tag="LTU54_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL8296137.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            25216..26268
                     /locus_tag="LTU54_001416"
     CDS             25216..26268
                     /locus_tag="LTU54_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBL8296138.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            26270..26587
                     /locus_tag="LTU54_001417"
     CDS             26270..26587
                     /locus_tag="LTU54_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296139.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            26714..27337
                     /locus_tag="LTU54_001418"
     CDS             26714..27337
                     /locus_tag="LTU54_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HBL8296140.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            27352..28242
                     /locus_tag="LTU54_001419"
     CDS             27352..28242
                     /locus_tag="LTU54_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HBL8296141.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLFGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            28265..28480
                     /locus_tag="LTU54_001420"
     CDS             28265..28480
                     /locus_tag="LTU54_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296142.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            28492..28833
                     /locus_tag="LTU54_001421"
     CDS             28492..28833
                     /locus_tag="LTU54_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296143.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            28833..29204
                     /locus_tag="LTU54_001422"
     CDS             28833..29204
                     /locus_tag="LTU54_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296144.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            29197..29592
                     /locus_tag="LTU54_001423"
     CDS             29197..29592
                     /locus_tag="LTU54_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBL8296145.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            29594..29971
                     /locus_tag="LTU54_001424"
     CDS             29594..29971
                     /locus_tag="LTU54_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296146.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            29972..30580
                     /locus_tag="LTU54_001425"
     CDS             29972..30580
                     /locus_tag="LTU54_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8296147.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            30580..30921
                     /locus_tag="LTU54_001426"
     CDS             30580..30921
                     /locus_tag="LTU54_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296148.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            31163..>33187
                     /locus_tag="LTU54_001427"
     CDS             31163..>33187
                     /locus_tag="LTU54_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBL8296149.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANSFVLIIGAVVGAI
                     AVFGYFMATNEEFRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNEIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFS"
BASE COUNT        11502 a         5085 c         6824 g         9776 t
ORIGIN      
        1 taccgcagat gcaagctttt gaacaccgtt ttatttgtct cacacccttt tccttcctac
       61 ttgtaacctt gctcccgtgt tagaaacatg ttacactaaa tatgtgaaat tattaacaag
      121 gagcgtcgtt atgcgagaaa aaacagatac actgccaaaa gcaactgcga aaagattacc
      181 tttatattta cgctacttga agatgctgga tgactcaggt ataagtagaa tcaaatcaaa
      241 tgagttcagc gaaatcaccc aaattccttc tgcaacgatc cgcagagact tctcgcaatt
      301 aggcgagcta ggcagaagcg gatatggcta tgatgtccct tttcttatcg atgtattcaa
      361 taatatcctt aatactaaag aagaaaaacg aatcgcatta gtaggatatg gaaatcttgg
      421 caaagcattg aaacacaaca attttagaag aaatgaaaat ctaaatatcg tgtgcgtctt
      481 tgataatgat ccagcactga tcaatcgagt aattgatgga gaaatgatct atccgattga
      541 tcgtttcgcg gagatagcca aagcaaaaaa tgtgacagta gcaatctcta cggtaccaag
      601 taaatattca caatctgcta ttgatgaaat tgtcaaagga aatgtaaccg ctatacttaa
      661 ttttgcgcct gacagagtaa ctgtcccagc ttatgtcaat gttcaataca ttgatttgac
      721 aaccgagttg cagacgctta tttactttga tgagaattac tcagaagttt tttcttagga
      781 aaatttgagt aggcttattg atcttgcgag aactcgtcgt aattctttca aaaatttttt
      841 agtactaaat gaagtggttt tttcacgacc acttgttgac taaaaaaaaa aaccattgta
      901 gaatgaagca taacgctgaa caagaggagt aactggcaca ttcattctta gagagagaac
      961 tatctgctga aaggttctca atgacaacca gtgaaggtag cttgggagtt tctgtcttga
     1021 atggagtaga gatagacgag tcgtgatcgt taactcactt gagaggcaat aatgattgat
     1081 tattgcaatt taggtggtac cgcgtaaatc atacgtccta gaatacgaaa gtattttagg
     1141 acgtttttta gtttaaagga ggaaaacagc atgacggaag aaaataacct atcaacaaaa
     1201 tataatccgc aagaagttga agctggacgt tatcagcatt ggttagaaca agatttgttt
     1261 aaaccgagcg gagataaaaa agcaaaacct tattcaatcg taatcccgcc cccaaatgtt
     1321 actggaaaac ttcatttagg gcatgcttgg gatacgacct tgcaagatat gctgattcgt
     1381 caaaagagaa tgcaaggata tgatacttta tggctgcctg gtatggacca tgcaggaatc
     1441 gcgacacaag caaaagtaga agcgaagctt agagagcaag ggatttctcg ttatgatcta
     1501 ggtcgtgaaa agttcatcga tcaagtgtgg gaatggaaag aagagtatgc gagccacatc
     1561 cgcgaacagt gggctaaaat gggcttatct ttagattaca gtagggaacg ttttacatta
     1621 gatgatggat tatctgaagc agtacgtaaa gtttttgtta ccttatacga aaaaggtttg
     1681 atttatcgtg gtgaatatat catcaactgg gatcctcaag cacgaacagc tctatctgat
     1741 atcgaagtta ttcataaaga tatcgaaggc gccttttatc atatgagcta tgaactagca
     1801 gatggatcag gcgtagtaga aattgcgaca acccgtccag aaacgatgtt gggagatacg
     1861 gcgattgctg ttcatccaga agacgagcgt tatcaagcgc tcatcggcaa aaaagtgatt
     1921 ctacctttag tcaataaaca aatcccaatc attgcagatg aatatgtaga tatggaattc
     1981 gggacaggtg tagtaaaaat cactccagca catgatccaa acgactttga ggtaggtaat
     2041 cgtcatgatc ttccacgcgt aaatgttatg gatgatgatg gtacgatgaa tgaactagca
     2101 ggaaaatatg ctggaatgga acgttttgct gctcgtaaag caatcgtgca agatctgaaa
     2161 gaaatcggtc gattgattaa aatcgatacg atgatccaca gtgtgggtca ttccgagcgg
     2221 acaggtgtcg ttgttgaacc tcggctgtct actcaatggt ttgtaaaaat ggcgccgtta
     2281 gcgaaaaaag ccattgataa ccaagaaaca gatcaagcag tggaattctt ccctccaaga
     2341 ttcaatcaaa cgtttttacg ttggatggaa aatgtccatg attgggtaat ctcacgacaa
     2401 ttatggtggg gacatcaaat tcctgcatgg tatcacaaag aaacaggtga aatgtatgta
     2461 gggatggaag caccagcgga tagtgaaaac tgggtacaag atcctgatgt tttagacact
     2521 tggtttagtt cagctttgtg gcctttctca acaatgggct ggccagatac ggaaagcgaa
     2581 gattataagc gatacttccc tactagcact ttagtaaccg gttatgacat cattttcttc
     2641 tgggtaagca gaatgatttt ccaaagcttg gaatttactg gcgaacgtcc atttgagaat
     2701 gtcttgattc atggattgat tcgtgacgaa caagggcgga agatgagtaa atcattaggt
     2761 aatggaatcg atccgatgga agtaatcgaa aagtatggag cagatgctct ccgctggttc
     2821 ttatcgaatg gttctgcacc aggacaagat gtccgcttca gctatgaaaa aatggatgct
     2881 tcttggaact ttatcaacaa aatctggaat gcttcacgtt tcgttatcat gaatatcgaa
     2941 ggaatgaccg tagaagagat cgatttatct ggagaaaaat cagtggctga ccggtggatc
     3001 cttactcgtt tgaatgaaac gatcgaacga gtaactgagc tatttgatcg ttttgaattt
     3061 ggtgaagcag gtcgacaact gtataacttt atctgggatg atttttgtga ctggtacatc
     3121 gaaatgagta aagaagtact ttatggagat gatgctgttt caaaacaaac cacccgtagc
     3181 atcttagtcc atacacttga tcagatccta cgtttattgc atccaatcat gccattcgtt
     3241 acagaagaaa tatgggaaca tattcctcat cagggaaatt ctctagtagt ggcagaatat
     3301 cctgttgtac gtccagagtt tgatgatgaa acagcttcta aagggatgga agtattgaaa
     3361 gaattgattc gagccgttcg aaatattcgt tcagaagtga atacaccatt gtctaaacca
     3421 attacgttgc taattaaaac aaacgatgct aaaatcgatc aatttttagt tgaaaataca
     3481 agctatatcg aacgcttctg taatcccgaa gagttgacga tatcaagtga gatcactgcg
     3541 ccagatctag cgatgtcagc tgttctgaca ggtgcagaaa tcttcttgcc gttagcagga
     3601 ttgatcaata ttgaagaaga aatcaaacga ttagaaaaag aactggaaaa gtggacaagc
     3661 gaagtaaaac gtgtccaagg aaaactttct aatgaacgtt ttgtttctaa cgcgccggaa
     3721 gaagtagtag aggcagagcg tgcaaaagaa aaagattatt tagagaagca aacggctgtc
     3781 aaagaacgaa tcgaaagttt gcgtactatt caataagaat agaaaaagag agtgtgaaat
     3841 cagctagcta ttactaaaag ctagctgatt gaacatcgtt acaggaaaga gtttgagcct
     3901 cactcttagc ttcgattcat aaggagatgt gacataaatt ttgtcgcatc tcttttttag
     3961 tctactctta ttatgtagga gaaggaggga ggtggatcat gtatcaagag tcaataaagt
     4021 agttgataaa atcgttcctc tctctgtttc ttttctcttt gtcatacttt tatttttctt
     4081 atgtgttcta tttgttataa taaaagagaa atgggggaat tgttgatgac gattgaagaa
     4141 gcaatagaat ggatccatag tcgattacca tttggttcac gtcctggcct tgaccgagtc
     4201 gaagctttat tagaagtggt agggcatcca gagagaaaag taaaaacgat ccatatcgct
     4261 ggaacaaacg gaaaaggttc tactgtagct tatttgagaa gcctattaga agaggctggg
     4321 ctgacagtag gtacatttac ttcaccttat atcgaaacgt tcaatgaacg gatcgcaatc
     4381 aatggcaaaa gcattccaga caatgatttg atccagcttg taaaaaaata tcagccactg
     4441 atcaaacaac ttgatcaatt tgaagcagta gctggtatta ctgaattcga gacattaaca
     4501 gcaatggcat ttgattattt tttgatgaag aaagtggacg tagcgattat cgaagttggc
     4561 ttaggcggct tattggattc taccaatgta gctgtaccaa tgctgacggg aataacaacg
     4621 attggacttg atcataccga aatacttgga gatacaattg aagaaatcgc tgcacaaaaa
     4681 gctggaatca tcaaacaagg cgttccagta gtaacaggaa atattgcccc agaagctttt
     4741 actgtcatcc aacaaacagc taaagcaaaa aatgcaccgc tttatgtata cggggaaaat
     4801 tataaaataa actatctgca tcctgatgag atgtggggag aagtatttga attttttaat
     4861 gaaataggaa agatccctca attaaccact ccgcttttag ggcaacatca agctgaaaat
     4921 gcaggagtag ccatcgaatt gtattttgtt tactgtcaat tggaaaagct gcctttccga
     4981 gaaaaagatg ttttgaaggg attgaagaaa tctcgatggc caggaagaat ggagcgtttg
     5041 agtaacgagc cattagttgt tttagatggt gcccataata cgcatgcaat gaaccggctt
     5101 gttgagaatt tgaaaaaaga attcaaagga tatcaaatca acattttgtt ttccgcctta
     5161 cgaactaaaa atgttagaga aatgctagaa cgattgcagc aagtaccaaa tgtccatatt
     5221 tatttgacga cctttgaaca tccaaaagcc atcgaattaa gtgaagtatc agatttagca
     5281 agtgagagag tatcaatcgt ttcattgtgg caattcggat tagcagatat attggaaaag
     5341 atgacaaatg aagacgtatt agttgtgacc ggttcacttt atttcatttc agaagttcgc
     5401 catttattaa tacaattagg aggagatcat gaagtataat gcagtgattt ttgacatgga
     5461 tggtttgctg ttcgacacag agttagtgta ttacgaagcc acacaagtag tagcagacaa
     5521 gatgggtttt ccttatgata aagacctgta tttaaaattc ttaggtgttt cagatgaaga
     5581 agtatgggcg aattatcata tgattttctc ggaatttggc aaaacaagtg tacaaagatt
     5641 catagatgat tcataccagg aaacgcttga acgtttttcg caaggagcgg tacaaatgaa
     5701 gcccggtgtg ttggaactcc tctctttttt agaagaacat cagattccca aagttgtcgc
     5761 ttccagtaac caacgagcag tcattgaact tttattgaag aagaacaatc tgtttgaacg
     5821 atttgagacg attgtttctg cagaaaatgt tcaacgagca aaaccagacc cagaaatttt
     5881 cttgttagct aatcagaagc taggtacagc gaagaaagaa acattgattt tagaagattc
     5941 acaaaatggt attttagcag cttctgcggc tgagatcccg gtagtcatgg taccagatct
     6001 gcttccacca tcccaagaat tgatcaaaaa aacagctggg gttgtttctt ctttacatga
     6061 aatccctgca ttgttaaaat aaaaatccat ctcttttcgt atcttatttt agataagata
     6121 gaaaggagat gtttttttgg gaaaacgatt gatcaaagaa gtacctacta gctctttacc
     6181 aagggaacgt atggaaattt atggcgcgga agctttatcg gatcaggagc ttttggcaat
     6241 cttactgcgt accggtcaac acccttatag tgtgatgtcg attgctggaa atctgttaaa
     6301 aacgtttgga ggactggcct ctttacgaca ggctactttg catgagttag aagagattcg
     6361 agggatagga cgcgtgaaag caatcgaaat caaagccttg atcgaattag gtcgacgaat
     6421 tcagacggat catgaaagag cggctccacc ggttcgtacc agctacgaat tggcccagca
     6481 attgattctg gaaatgaaag atcacaaaca agaacatttg gtttgtattt atttggacac
     6541 gaagaatcaa gtgatcttaa aaaaaacagt gttcatcggt tcgctgaatc agtcagtcgc
     6601 tcatcctaga gaaattttcc attatgctgt acgttattgt gcagcaagga tcgttttagc
     6661 gcataatcat ccttccggaa tatataagtt agtacacata aattatataa ggaaatttac
     6721 tgttatattg ccatctggaa atactattat gttatcgata attgaacgcc ataatgcatt
     6781 tttttcttct tccgaagatt ctttataaat ttcatcgaat tcttttgata gaataaattc
     6841 gacatttttt atatctattt tagctggctc tacttcaatt tcatttaatt ctttcattaa
     6901 agcttctcga tcttttttgt attcctctaa gtctataact tcattcaagt aggctttctt
     6961 caatctatct aatttattct caatattttt cttactattt ttctttttaa taatctttgg
     7021 ttgttttttt gaatagttta tttgtatttg ttcaaatttt tgtttagtag cttcaaaaat
     7081 ataacgttct aaattttttt cgtaaaaagc gcgcttattt gtgcaacgta atttattatt
     7141 tcttcgtttt acacatctat aacaagtcct ttcgtatata gattctgtac cgtctttttt
     7201 cttatatctt gctttaatag tttgaccagc tactgatccc tgacagtcag aacattttaa
     7261 taaaccttta aaaatgtaat cacgttttgc atttgttttt aaatttttag gtaaggcttt
     7321 ttgaacagca taaaaaagtt ttttatcaat aataggagga cagtaatttt tattatttct
     7381 taattcacca atgtattttt tgttcgtcaa catattttta acactttgat aatctctaac
     7441 gattccatac tgagagccta aataataaac agtttttctt aaactaccac tagaaagaaa
     7501 gtagttgaat atatctatca cgataggagc atcatcattt attactaatt tcttattttc
     7561 tatagagtag cctaacggag ttttcccgct tactacttca ccttgtgcaa ttttatttgc
     7621 cataacagct ttgatgcgtt ctgaatcaat ttgggcttcc aattcagcaa aactcattac
     7681 ttgtgcaatg aaagttctgc catacgctgt agttgtatca taatattgtt gagaaacagc
     7741 attccaagaa acattgtgtt tttctaaaat ttcttgagtg tttaaataat gtcgtagatt
     7801 tctaaaccat ctatctaatt ttgtaaataa aattagatct attttatttt cttttacatc
     7861 ttgtaatagt ttttgaaatt catcacgatt tattttttga ccagaaatac caccgtctac
     7921 atattcatta actactttca aatccttgtg ttgttcgata tattcataga gggtttcttt
     7981 ttgttctcta agactatctc catgtttggc ttgttgttca gttgatactc tcatatataa
     8041 tgctactcgt ttcatgttat ctcctttcga atgtatgttc ttatcgttgt aaaagaaaag
     8101 cccgaaggct aatcttttta ctacacatct gttaattcta atgatcttct gtattgttca
     8161 gcttgagcta gtttagtaaa agatacagtt ccgttataat tttctaaaac aactttttca
     8221 atttcatcaa gagatacttt aaagaattct tttcgttgat taactttatt gacttcgttt
     8281 tttctaaaat gcgaatgtag ttgattttct aatgttggtg catcttctga aaatatcatt
     8341 gcatgaacat caaattcaaa aggtacagaa gcgctactaa gttccttaat tctatccata
     8401 ggttctaacc gtctagtcat tcctatttta tagacatctt caccgaaaga tccgatatta
     8461 ctgatgacat aaacatagcc agctctagta ttaagttctc tttgcaatac atcttctttg
     8521 acagtttcta gttctcttaa tttctcttcc agttctttaa ttttatcagc atagagttct
     8581 ttttcaacat cagcactagc tttttgcatg tatgccatta acttagttat ctctgagtta
     8641 aactgtcgtt cttctttatc aatgcgtttc ttttcacgtt ctatttcacg tcttaccttt
     8701 tcttcttcaa tcatttgttc ttttattgct ttttgctgtt ctttttcatc gttaacttta
     8761 actatgtatg aatagttgag ttctaattct tgaagtttta attcaagtaa ttcttttttg
     8821 aattctgctt catcgttttt aaaaagcttg tttattttat caaaagttct tataattttt
     8881 tctctagttg aatctacgtt agaaatagta agtttattga gaagtccagt tatttctgat
     8941 tctaatggta taagcatttt ctttttaata ttattagctt ctctattatt caaataagaa
     9001 ggaatattaa ttgcaccgtc taaaataaaa tccttttctt taatctttaa taaagaaagt
     9061 ttgtttttta tttcggatga agaagtttca tctatattcg gaataattac tgatttatag
     9121 atagtgacca gagatgtgtc atatttttga ttaatttcat cgtctaaccg ctggctttca
     9181 gcattaagaa tatcaatttt gttttctagc gttgacaagt ttttattcaa tttacttatg
     9241 tttttctctt tttcagatat aaacgtttcc ctagcattta attcttgaga atattccttt
     9301 accttactct ctaaggcaga agttttatct tttaattttt tagcttcaga taatttgcta
     9361 ctacccataa ttattaaaac tattccaact aaaaacaata gtactcccat tcaaaacgcc
     9421 tcgcttcttt tataaaataa ttgtcaaaat catataaacc cttaccgaag ttgacggctc
     9481 cggcatggga atttttttaa aatacaactt ttccgataat tctaatctgt tctggcgata
     9541 caattctatc ttcgtataag tcgtttatag aacgtaaaat gattgtttga ttttcgtaat
     9601 ctttatatat ctctttacat gtaactccgt caccacctaa ttccacaata gcaatttccc
     9661 catcttctac gtccggttgt tctttaacga atacgaattg tccattcctg ataacaggtt
     9721 ccattgaatc gcctataata ggtactgcga agtcagcagc acttggaact ctctcaaatt
     9781 cttcctcttc gacaaccgta tcaccgtaag taagttctgt tggattggca gccgaagccg
     9841 aattgtgaac agttgggatt ttttcttttt taggaaaatc taggacattt tcttgttttt
     9901 gttcttctaa tcgattactt gcaaattcat aggtagcaac tttattttcg tcgttaagct
     9961 gattatatat aggaaggata ttatatatct gattagatat agataaatca atattcatca
    10021 aatcatctat atcaacattg aatatcttag ctatctcatt taaggttttt aattttggtg
    10081 tatattttcc tttttcccat tcgcttattg aggatgaact ttttcttcct agtttttcgg
    10141 ctagttcttg ttgatcaata ttatactttt ctctaagaaa ctttagattt tcagcgaaca
    10201 tagtaacacc tccttgagtt aattgtaaca ccgtttcgga aaaaatgtaa caggtttctt
    10261 agaaaatatt ttagtgtttt taaagaagta tttacgcaaa atagaaatcg atttcaggaa
    10321 aactgaaata tgcacttgac ttcggaaaaa ccgaagtata taatttagtc agaaagttaa
    10381 agagaggagg cgaataaaat atggataccg cgaagaaaga aaaatataca ttgagacaat
    10441 taagagcttt aaagggttat tcaaaagaag aactgtcaag gaaaagcaaa gtgacatcaa
    10501 gaacaatatt tatttacgaa aacgatgtag ataaaatgag gaacggaaag tacgccactc
    10561 tagaaaaaat tgctaaggca ttgggtgtta gagtttccga tattttttta gaccctgatt
    10621 cggaaaaacc gaaatcaata acaggtgaaa caaaacaagt cagctaggag gtgtgaatag
    10681 tggaagagaa gaatataaaa gtaccgactc ttgaagaata tacaaaaatt gttgtcgaaa
    10741 cagacgagca gaacccgaaa acaatcgcag ttattacggc gaatgatatt gaatcatgcg
    10801 aaggttttcg agttcgaatg acccctagat atgattaatg ttctttatca cgtggaggta
    10861 taggatcgtt tccaaaacta tcttttgatt gtatttgtcc attctttttt tggacaataa
    10921 actctgattg ctgtttttta gaaatctttt taccatactc aacagcatct gatttattat
    10981 caaatagttt tgtatcttcg gaattacctt caccacgtac agcccatttg ccatttctag
    11041 gagataccca ttgattttta cccataacaa ttcacctcgc tttcaagtta attctattaa
    11101 gaggtgaaaa gaaaacaaac tgatggaggt gagattgtga gtaacaagcc aaaaatcact
    11161 ttgaatggca cacctaatct tcaaagatta gcagatgaaa tggctagaat acgttctaga
    11221 cattggggag ttactgtggt ggcaaccata attgaacaaa aagaaaagcc cacacagccg
    11281 gcaagcaagt agtgggcacc atagaacaat aatattatat cacacactcg ccacttccga
    11341 aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttctt cattttggca
    11401 actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa
    11461 tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa
    11521 ggggaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt
    11581 cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca
    11641 cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa
    11701 atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa
    11761 tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt
    11821 cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc
    11881 aataaacgaa aggagggatc aaatgacatt acaagagcat caacacttga tgcgaaaact
    11941 aaatcaggaa taccacgagg aaatgaaacc tagattgcta ggggaacaat ggcgtgaacg
    12001 ccagaagaag tggttagaaa taaaaaagtg actcagccgt ccaaagcaat gagtcacaaa
    12061 ccaaaaatta tacctaagga gattttatca catgaataag aaaattgaaa atcttatcga
    12121 agaattgaaa cgtgaatgcc aaaagcaagg tgtttctatt atttgtacag cccaaaaaga
    12181 aggggaactt aaaagtctcg tttatggtga gacaacagaa attttacttt gtcttgctat
    12241 gcaagaagaa catttagatg aaaatctccc attgtcagca catattatgc gtagaattgc
    12301 tgtggatgct tacgaacaag cgaaaaatga agaagaaaat caaccttcaa atcatacttt
    12361 tgttattaat aacaaggaag atttagctga tgtgatgact cgtattctca agggggaatt
    12421 tcaatgatga atgaaaaaac aattgatcta ctcattattt tatttttcac gttagtgatt
    12481 gtagcgaaaa gttatgtaat ctttggcttg ttattcgcaa ttaccatgct tgtgctttgc
    12541 gtgttagcaa gcagaaaaga ggttaaacat gaacgagtta gcaaatcttg acaactattt
    12601 aactgatcct gattatgcta aaccaccata tgaagcgcca attgatgagg aggatgaaga
    12661 tgagtaaatc taccttagaa atgagccatc aagaatggct cgaagaccgc aaaagaggta
    12721 ttggtggctc agatgttgca accgtacttg gattaaacaa atacaaatct ccttatcaat
    12781 tgtggcttga gaaaacgggt caaattgaat tgaaagattt agaaagcgaa ccagcttatt
    12841 ggggtaatgt tttagaagaa gtggttgcta aagagtttca ggaacgtaca ggcaaaaaag
    12901 tacgcagaag aaaccaagta tttgaacatc cattacatcc atttttaaga gcaaatattg
    12961 atcgggaagt agtgggagaa aatgccattc tggaatgtaa aacagccaat caatttctcg
    13021 gaaaagagtg ggaaggtgaa gaggtaccgc ttagctatct ctgtcaagtt cagcattaca
    13081 tgaacgtttt aaacaaagac tattgttata tcgctgtttt gatcggtgga caaaaattca
    13141 tttggaagcg gattgaacga gatcaagagc tgatcgatac aatcactgaa caattagtag
    13201 agttttggga aacgaacgtt cttggaggta tcgagcctgt aattgacgga agtggagcga
    13261 ctgctgactt cttaaaagaa aaatatgcag atgtagaaga aaatcaaaca gctctaccat
    13321 cacgttttga tgaacttatc gagcaaaaaa gagaactcaa gcggacgaaa aaagaaattg
    13381 aatcatctat tcgtcaagta gacaacgaga tcatcagtga gctaggtaaa cgtgaagcca
    13441 gtatcggtat cacacaaaga aacattatca gctggaaact tgttcgtaca agacggatga
    13501 acacgaagaa actagcagag aaatatccag atgtcgcaaa tgatgaagag atttataacg
    13561 ttactgaatc aagaaggcta accgaaaagg agatcaaata atatggcaac aaatgaatcg
    13621 ttaaaaaatc aattggcaga aaagccacag aaacaagttg caccaggaca gttagggctt
    13681 aaagctctaa tgaatacacc aacaatgaga aagaaatttg aagaagtact tcatgacaat
    13741 gctaatgctt ttatgtcgaa tgttatgact cttgtatcta atgacagtta tcttgcagat
    13801 agtgaaccga tgtctatcat gagtggtgcg ttaactgctg caacattaaa tcttgggcta
    13861 gataagaatt taggttatgc atatttagtt ccattcaata gtaaaaacaa gcaaacagga
    13921 aaatgggaaa agaaagctca attcatgctt ggctataaag gatatatcca attagcccaa
    13981 cgatcaggta aatacaaagc attaaatgtg atcgaagttt acgaaggaga actaaaaagc
    14041 tggaaccgac tgacagaaga gtttgagttt gatccaaatg gtagaacgtc tgatgaagtc
    14101 attggatatg ttggctattt cgagttactg aatggattca aaaaaactgt ctattggacc
    14161 aaacaagaaa tcgaagctca tcggattgct aacaataaag atcgcgataa gacaaagtta
    14221 agtggtgtgt gggcatctga ttacaatgca atggcacgaa aaactgtttt gagaaatctt
    14281 ctttctaaat ggggaatctt gtccatcgaa atgcaagaag ccaccacatc ggatgagaga
    14341 gtccaaaggg ttcaagaaga cggcagcatt attgctgaaa cagaagttga agaagatatt
    14401 cctgaaagaa aagaagcaga ggttatatct gaagaaaatg aagatgtaca aactggatta
    14461 tttgatgcat ctaatccgcc gttaaacaaa taatgaggga gctttctccc ttgcttttct
    14521 agaggagaaa tacgaatgaa cacaggatat gtcaaattac atcgaaaagt gatgaattca
    14581 ttcgtttgga ccaatcctta tatgtataaa ttatggagct tgtgtttgat gaaagcgagt
    14641 catgagaatc gcaagatact ttttaatgga aaagagatac aagtgaacag cggagaattc
    14701 gtcacagggc gcgatgctat cacatctgag atgaacgaag gcgtgaaacg tgaacatcaa
    14761 gtgaacagcg gttctgtatg gagatggcta aaacaatttg aaaaaaacgg aatgttgaac
    14821 atcaaatcaa ctacgaaata cagcgttata tcaataaaaa actggtcttt gtaccaaagc
    14881 agtgaacaac aaatgaacat caaacgcaca acaagtgaac aacaagtgca cacaaacaag
    14941 aatgaaaaga attataagaa tgaaaagaat aataaaacat catcgtcgca accacgcaaa
    15001 aagcgtgttt acgacaccga ctcagtttac tacattctcg cggaggagtt attcaaacag
    15061 atttgtcaga atcaggaaat caaaaagccg aatctgcaaa gttgggcaga taacattcgg
    15121 aaaatgatcg agatcgacaa gcggaccgaa aaccaggtac ggggaatgat tgaatggagt
    15181 cagcacaatg tgttttgggc atcgaatatt ttatctgcta aaaaattgcg agaaaaatac
    15241 gacacaatgg cagcgcaagc aaatcgtgat tataaaacaa aacaaactaa aacgcttgaa
    15301 tacgagaaat ttagcacaga tgagttgcct atttgagagg aggcgtaggc atggaaactg
    15361 ttggggaaat catggaaaag ctgatacaga aagtgcttgt ccaacgtggc gaatgtcctg
    15421 aatgcggaca gcctttgtat ggatggcgta cgaagaaccc tgatggctca gaacgttgta
    15481 agccaacctg tatgcaatgt gggtataaag cattgcgagt tcaggaagac ttacaaacag
    15541 aacgaattta caacgagagc ctgaaagcaa gagcaatcaa ttttttcaaa ggtggttctg
    15601 ttgtgcctaa tcaagcgttg tttgattgca cattgcagaa ttatcaaatt gtcgatcaag
    15661 aaacaagaca agcggttgaa gtaaccaaac gctttgttaa ttcagtcttg ttaggaaatc
    15721 caagtcacct tgttttaact ggaaaacaag gaacaggtaa aagtcaccta gcgatggcag
    15781 cggcttggga agtcttgaag cgatcaaact acgataaaaa aatcttattt atcgggttac
    15841 aggaaatgct ggatcaaatc aaattttcgt acaacaatcc tgaactcaga aaaacgattg
    15901 agggatcgtt gattgcagat atcaaaacag cggatttagt catcattgat gatatcggtt
    15961 cagaactagg aaaagatgca tcagatagtc gagcgtttgg cataaacacg ctaaattcgt
    16021 tcttggatgc acgacagaac ctagcaacga ttatcacaac gaacttgctt ggtgaagaac
    16081 tgaaaaaagc ttacggtacg agaacaatat caagaatgtt tgtcaactct gatggattta
    16141 cgatggtatt ttctcaaaca gcagacaagc gcataaaacc agtcaaagga gcgatcgcat
    16201 gaatttgact aaacaagaaa aaagaatctg tcgtcagata atgttgcaaa ttataggtga
    16261 tcgtgacatg gacgaattat ctcaacaaga gagtcgaatg gttcggaaga taagtaagct
    16321 aatcggtgca gatacgatgg atcgatccaa gccattaccc aaactgaatt ttgaatcatt
    16381 tacgtttgag aaatatagcc atctgagaga ggtaggctat agtgtaaatg ctattcgaaa
    16441 agcacttggg gtaggaagta gtgtatgggc aaaatggcgg aaagaaaatg taaatgaggt
    16501 aaatcaaaat gattaagcta ataggcaaag agacgctcaa ggtttactat gtagcggaaa
    16561 caaaggcaga agtcagccgt tggctattga aaatgtttaa taagactaga gccaatgctt
    16621 cagtaagtca gaaagcaagt gtatcagaaa caggcatgcc tgaagcaatg tggatttcaa
    16681 ggaggaactg agatggcaat gataccgaag tttagagcgt gggatagaga aaaagacaga
    16741 atgatttatc ctagcacgac aggagtttgt tttgaaatga atgatgatgg tataagtgtg
    16801 cttgatattg atgtagatta tcccaatgac tatggttttc ccaaaatagc ttcaatcctc
    16861 atgcaatcta ccactctgaa ggataagaat ggtgtggaga tatttgaggg ggatatagcg
    16921 cttatcactc ataaatcaac gaactacgcc gatacttatt ggcacagtta cgtagaagtt
    16981 tttagagcaa aaaacggagc ctacagaatt cgcggtgaac acatctacga aacagaatta
    17041 tatagtaata gaaaacaatt gacggtagcg gggaatgttt ataattcacc agaacttttg
    17101 aagagagatc gatttatgac tactaccgaa aatccagagt tattgaagga ggaacagcga
    17161 tgaataaaca ggaattgatt gataaatata ctgcagaaat atctagatta agaccttatt
    17221 gccctaatag acatttaatt agcgatcaac ttaaatatgg tctttataaa gaaatcctag
    17281 aagatttaaa acaactagac gaaccgcaga aaccagcggt gcctaagttt gtggcggaat
    17341 ggatagaata cgctaaaaag aaaggcgata gtctagctat ttcattcaag ccgtggaacc
    17401 tctacggtgt tgagtatagc aagactgata gatggattga agataatcaa ggaacgtttg
    17461 ctcgtgcttg gctaattggc tacgaggtcg agaaagagcc gttgtattat gtgaagttgc
    17521 cagttgtgta ttttaatcat tgggatttag agacgtatct aatgaaagat gatagaggaa
    17581 atataacaat tgcagacaac aacgattttg atgatatgaa atttacggaa tcagaaataa
    17641 aagcaattga tgaaagatac tggccatttg ctgtgccagt ggaagaggtg gcggaaggat
    17701 gagcaatctt aaagttggag atattgtttt ttacaaaggt gacgaaggca tcatccgaga
    17761 agttatgacg gatggaaaag aaaattttta tagggtagat gtcagcagaa aatctatact
    17821 ctacctgtat gaagacgaat tagatttagg tactcaaaaa ctcaacgaca accagaaaat
    17881 ggtgtttgat tatcttaaat caaacgttga acaaggtggc aaatcagtta tgtattcgat
    17941 attccttcta ggtgattggg actctcgaat tggagcagca agaaacgttg atatagctta
    18001 ctgtggatta aatggtaagc aggaagcaga agtcctatca gcattcgctc aatggggatt
    18061 agaacaggag gaagcggaat gaaatacgaa ataccactaa gtgaagcaga cgtccagtca
    18121 attattaacg gtcgggaggt taataaaaaa cttcctgatg gtactgaatt agtcatcaga
    18181 caaagttatt tgaaagatat ggcagctcca gtattaattg atcgttttaa cgtgactgat
    18241 tctgtggtag agaaccattt aaaagaattt cgatcaagta tagacgacac tttcagatta
    18301 gggagttgat tgacaatgaa caacagacat cgcagaataa caaaactaag aaaacaggaa
    18361 ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat
    18421 aaagtggcaa gtcagtgtgt tgctgatgcg agtaatgcta ttcgtaagtt tgggatttcg
    18481 atattaaatg atgatcgtaa atgggaggaa atgagatgaa actaaaagac ggattttacg
    18541 ctagtagtca cggcatcggc ggtttaatgc tagatatgcc gacaaagaat cctaaaacac
    18601 gtaagaaacc aaaagtcaaa gtcggtgaca tggttcgctg cgaagcagaa gggttcatct
    18661 atccatttcg tggatatgta gaacacgtct ataatcactc agcaatcatt cgtattgaaa
    18721 acacgatgga atgtgataaa tggacagcta aaagcaaaga gaatttagca gtggtgagat
    18781 tggtggatat tgaactaatc aatgacaaat aaaaaagccg gatcgctccg actgattcaa
    18841 taaattccac aagtttatta tatcacataa aaggggcggt ttgacttgat gcaattgtta
    18901 cgagaggtag ctttcaaaca gacaagatgt aatgcgagag atgtgctgaa gaactttcgg
    18961 cgtttggagc ggatggcagg tcgctctttg atagatatta agtcgccgat tattacggac
    19021 atgccgaagg caccgaagca cggcaataag gcagaagacg cgatcattca gatgatggat
    19081 atagaagcag agagagacgc gattttagcg gctttgatgg cgcttagtct gattagtcgt
    19141 cagatactct actacagctt ttgtgtgcca gatagcttct caaactacag gattagccgt
    19201 gaagtgggtt attcagaaag aagcatacaa cggatgaagt cggaagctct gatagagttt
    19261 gcagaagcat ataaacatgg gaaaataatt gcttataaat aggaggacta tataatgtgg
    19321 aaagattatg tatcattgaa agagttgaaa aaagatctta ttttcaaaaa aatcgtagaa
    19381 tggtcagaga gtgaattaat tttagaagat ggaaccaaaa tggaagttgt atgtagcgaa
    19441 tcagattgct gcgcatgggc cgaaggtgaa ttcaaaaatg taaagcttga tgcagtgatt
    19501 acagatataa agatttttga taaaggtaac cgtctctata atggcgatgg acatagttct
    19561 tatgccgaag tcgttgttta tcataatcga aatgagattg ctaaagcgga atgtacagca
    19621 aacgatggga atggaggcta ctattacagt gtctgtgctt taaaagtcaa agaaaaactt
    19681 tgtgtagtga ctgatgcata aaaaatggcg gttttttggc ggaatgatgg cggtttttag
    19741 ctatttacca gtgatattat ggtagtgtcg aaagattagt gataggtctg agacaaaata
    19801 aaatgtaagg gaggaaatct ccctcatcgt ttaattaagc ttcgatagac agcagcggaa
    19861 atattaagaa taaggatgtg aattttaact ccttctaaat tgttcttatt atctatcatc
    19921 cgttgctgtc tattgttatt atgtcactgt ggcggaaagg gtagacgctt aaaaataagg
    19981 tcaatacgtc gagggatagc cttaacgttt tatgatttga ccatgcaagg ttcgattcct
    20041 tgccagcgac ttagacgaaa ggtagcttaa atattaacca catcaacatc gtcagcgagt
    20101 tgataaaggt aactagcaga ctctaggatt taagccagtc ttaccgatta gtctgttata
    20161 gtagcaagct tgctagaggt agctcctctg gttgacgtgc agctcagttg gtagagcgcc
    20221 tgacttttaa tcaggaggtc gccggttcga atccggtcac gttaataaag aacctacgga
    20281 aacaatcaat cttatcggat gccgattggt tggctgaatt aagcagtgtg tctgtgaatc
    20341 agttaatagg cataaaatac tagcacagac gtgtgccact ctcaggtgta ggttaggaga
    20401 gaaacattag caaccgaggg ttggaaatgg gcgctcaaag tacacgagca aggcgaggtc
    20461 gatagtaatc gatggaatcg gtgtaggttg ctattacata attgattggg ttagattgag
    20521 ttttgggatt cggtacaaat gaatcgtcaa atgactcaag cacaggatcg gaaacgtccc
    20581 tgcctgtgca ttacatatta gatcactcgt tgagtggtct ttttatttta gaaaagagag
    20641 gattttgaaa atgaatcatg agaagtttat cgaaaaatgc aaggctattg tacgtgaaag
    20701 aattgagaat gagattgctg acctaagtgg agcagtacct gagtttagtg ttttcatagt
    20761 ctggtcatgc aaaacactac agaatagcaa agcattagtc agcgctagct taaaaggagc
    20821 accgtatttt gaaattacgt tgaacgggga caaaggtgaa atctatgtag atacttatct
    20881 caaaaaatca aatgaatgta tcaaagtcta gcaggtgcta ggctttttct ttacataaag
    20941 gaggaatgat ctcatgaaac aatataccgc taaagatttc gaggaaatga agcgattaaa
    21001 gaaggactat gaagaagttg atatggagct aactgttgga gtcattcaac gaagactgcg
    21061 ggtcggatta gagacagcaa aggctattta caatgatcta aatgctattg aagagaagaa
    21121 tggctaatga ggaactactg gtatatatcg ctaactaatg aatatcctcg aaccattgat
    21181 gattgttcag tgcgtgttgt gcgttctgta caaatcaaag ggaagtactc tattgtcgaa
    21241 atggggagag aagcagaacc atgtgagatt gatgcgtgca agctcgttta ttgcggtcat
    21301 ggtttctatg atgaaccaaa cattcaaaat aatattaaca agaatttgag ggattagaat
    21361 gcaagaaatg gcttaaaaat ctccaatcag gacaaatcaa gttagacaaa gtttccgatt
    21421 taaagatatt aattgaagca gatctaatgt tgaaagatat tgaaaattag aaaacaaaac
    21481 tcaacctaag aagattgcga ggtggtgtgt attgaatggc aagacaacgt gatccaagac
    21541 gtgatgaagc caaaagaatt tggttagaat ccaacggaga aaagcagtta aaggaaattg
    21601 catctgaatt aaatgtttca gattctcagg ttagaaaatg gaaatcgcaa gacaaatgga
    21661 gcgctgaatt gaaaagtaac gttaccaatg gcaaaagtaa cgttactaat caaggtggcg
    21721 ctcctattgg taatcaaaat gctaaaggta acaaaggaaa tagccgagct tctccgccag
    21781 tgggtaataa aaacgctttg aaaacaggcg agtatgaaac catatttttt gagacactaa
    21841 gtgatgaaga gaaggacatc tattctagtc tgaatgataa tccttctttt gttttgtctg
    21901 aagaaatacg tctacttaag ataagacaat ttcgtatgat gaagagaatc caacaagctg
    21961 aagctggact aaatgatgaa gaagtcgaac gattgcagca gctaagaaag attaaaaatc
    22021 cgattgaaaa aaatggtaaa aagctagaaa tcaagcgtga ggttatgcaa gatgtgcaga
    22081 ttagcagaaa aaaacatcgc aaaattgatg atattctttc aattgaagat tcattgactc
    22141 ggattagcaa ccagttagct aaagccatca agcaaatgaa tgaactttat atgaatgaat
    22201 acagaactga tttaattaaa gctcagactg ataagatcca agctgagaca aatgaaattg
    22261 gcggaaataa ttcaggtgaa gaaatagaag aatggaaaca ggcagtttta aatgccgcaa
    22321 acaaacgggc ggtgaaagaa aatgaataat gaatttattc cttttgctga tattggttct
    22381 gccattgatt attactatga taaaccagta gctttttgcc aagatatttt gcatttgaat
    22441 cctgatgaat ggcaagaaaa tgttttaaat gatttagctg aattttcaaa ggtttctgtt
    22501 cgttctggtc aaggagttgg aaaaacagca ttagaagcag gagcaatact ttggttctta
    22561 acgtgtcgac cctacgctaa agtaatagca acagctccga caatgaagca actttacgat
    22621 gtactttggg cagaggtagc taaatggtta aatgatagct tgatcaaaaa cttactgaag
    22681 tggacaaaga ccaaaattta tatggttggt gattcagagc gttggtttgc tacggctaga
    22741 acagcgacta aaccagaaaa tatgcaaggt tttcacgagg accatatgtt gattgtggta
    22801 gatgaagctt ctggtgtgtc tgatccaatt atggaagcta ttcttggtac gctatcaggt
    22861 tttgataata agctgttgat gtgtggaaac cccaataata ttgaaggtgt tttttacgat
    22921 tcccacaatt cagaccgtga taaatacaga gttcataaag tatcaagcta tgatagtaaa
    22981 cgtacaaaca aagacaatat agaaatgatt cttaaaaaat atggaaaaga aagtgatgtt
    23041 gctcgtgtcc gtatttttgg agaatttccc aaaggtgcgt tggattcatt tatcagtctt
    23101 gaaacggttg aattggctac agaaaaacaa attagtgatt ctttagtcaa taaaacaacg
    23161 gttgctcata ttggtgttga cgtagctcga tatggtgatg attctacgat tctctttcct
    23221 agaattgcta ccagggcatt ggagtatgag aagtattcaa aacgtagcac catggaaaca
    23281 acaggatatg tcatcaacat ggccaagaat ctaatgagtc aatatccgag tattgataaa
    23341 gtgatgatta aagtcgatga cactggtgtc ggaggtggtg taaccgaccg cctagaagaa
    23401 cttatagaag acaaacatta tccttttgag gtgtttggag tgaataacgg ttcaacatca
    23461 gaagacgatt tttacgataa tttaggtact caactatggg gaaacatcaa ggaaatgtta
    23521 gaagaaaata tgacagcaaa tcttaacgga gaacagcctg ttattgaatt gccttctgat
    23581 agttcgttaa tcaaagaatt aagtactcgc aaattcaaaa tgacaagtag aagtcgtata
    23641 cgtttagaaa gtaaagatga tatgaaaaag cgaaatattg gtagtcccga tattgctgac
    23701 gcactggctt tagcgtttta tgagccacca agtcactatc aatttattca attttaggag
    23761 gtgagctttt gattagtaca gtattatctt tagagagata taagaagcta cgtgttaaat
    23821 atgcaacaca aattgaagat ggaatgtttg atccgaatgg ttttatagaa gatatgaaac
    23881 cattttttgc tgatcgcgaa agaaaatatc tagcttatac tagcgaaaag aatgaaatag
    23941 ataatagacc aaagcctaac actgatattg taaaagttaa taataaactt catgctggta
    24001 tgtattcaat tgtcgtagac caagcagtca atcattttac tggtatacct atcaaatggg
    24061 attacgatgt atcggaacaa aaaagaacac tcattcaaag attaaaagat aaattcttaa
    24121 aaaatgatat agaacttccg acagttccag aagcttttaa caaattaaca agcaatcttg
    24181 attccatgag attcgcaatg cttgattctg aaactgcaac ttttcaagga gcttgtggag
    24241 tagctttccg attgttagag cctgtggaag aagatgatgg ttggaagtta agagcaagta
    24301 atattgaacc ttggagagca gaaagatatg gaaatgccgg aatctatatc aaagaaaagt
    24361 atgactctta ccagaaaaaa ttttttcaag aaatgaaagt tatgacgaga aataaaattc
    24421 tgacatatgc ttgctatggt gatttgaatt ttgttacaag cggaacgttc aaaaaaattg
    24481 atgagaccga taaccctttg gggacgatta ccttggcaga atttaaaaat aatacgaatc
    24541 gttattgtga ttttgaagta gctgaggaaa ttggagatgc aattgatcgt gctttgtctg
    24601 atcaacaaaa cgaaatcgaa caatttaagc ttgcgtacat gcttgttact ggcacaacaa
    24661 tgagcaaagg cactgctaaa gaaatgatga atcagctagg gattatcaac ttaaaagacc
    24721 ctactgcaaa agctgaatat gtcacaaaaa atttagcaaa agatttcaat gaatatcata
    24781 tggatctatt gaaaaaacag ttttacacca tttgtaaagc aatcgatttt aacgatgagg
    24841 tgtttaaatc aaacagttct ggagaagctc gcaagtggca aatcattagc ctagaagcga
    24901 aaacaaatac taaagagcaa tattttagag aaggattgaa agaatgcgca gaaacaattg
    24961 ctgcttttct taagtttcat gacaaagtag aaattgagcc tgaaaaaatt atttttactt
    25021 tctctagatc tttgccaact gatttaagtt atttagctga agctttacct aaacttgcac
    25081 catacgtatc caaacgaact attcagagcc aaattccatt tgtaacagat gttgattatg
    25141 aaaatgaaat gatggaattg gaaagtggaa gtgcttatcc agatagtgaa tacaattttg
    25201 gcggaggtgg caatagtgac gatagaaacg aagtattgga ccaaacgtcg agaactggag
    25261 gatcaagcaa ggctcaaaca agaaaatcag acacttaaaa aattaactag tgtatttcct
    25321 gaagcactga aagagataca agcaaaacta ttatcacaag ctgacttaca caatatcact
    25381 tatccagaaa tgatggagtt ttatagtaca agtaatcaga aaaaatatcg tgaatatgtg
    25441 gaaaaaaatt ataagtcatt aaaaatgtat gatgcaaaat acaaagagtt tatcgatgaa
    25501 ttttttccac catttgacta tgcaaaagtc aatcgcttat tacaaatacg atcagatgta
    25561 tttaaaattc ttgcagaata tgcgatggat gcagatgtga atcaatattt ttctgatcgc
    25621 ttagaggaaa ttcttcaaag aacatattct tctaatgcta atgtttttgt tcaactttta
    25681 aacgttgata taccaaatta tttaccagaa aatgaaatta aacattattt gaactatcca
    25741 tggtcaggaa agacattttc aagaagactt tggggaaata tttcatcgct tgaacagaaa
    25801 ctctctaatg ctattgtaaa aagtgttgct agtggagaag gcgttataca cgcattaaac
    25861 accatgagat tagactcaga aatttgtgac atgtttaagt tagaagaatc aaagtataac
    25921 aaagcgatag aaaatctcgt tcgaacggag tatgcaaaat ttgcacaaga tggtattgaa
    25981 aaatcatatt tagaaacagg tattgaggaa tacaacgtat tgactgcaaa agatgagaga
    26041 gtttgccgga tttgtggagg aaaggcaagt aagaatccct ataaactgaa agatgctgtc
    26101 ataggtgaaa atcgagcacc tttccatagt cgttgtagat gtacggatgt tcctaattta
    26161 ccaaaattag gaaaggatat tgatgaagaa tatgaccgtt tatttggcga tttattagat
    26221 gagtttgcac atgattcttt tggaattaat ttgaaacgga ggaagtagaa tgaaagattt
    26281 ttttgaagca gtactaacaa ttaatgtaaa tgctgatatt gcagaagcct acaaaacagc
    26341 tattgagtct gagaaccatc ctaatggctt gagagaccat tggaatggca attatgccta
    26401 cgtggttatt ggcgatcaaa ctgttaatta tcaagataat actccagttg ataagaatac
    26461 cgttaattta acgattcaat tattatctca ttcattacca aatttaaaag aaacagttga
    26521 ttggtatgaa aagatgggat gtattgttgt tagaactgac tacaaagaag gaaagtctag
    26581 taattaggct tttttatttt gtccgaaatg acgttaaact agcgcaatac tgggcttggt
    26641 tgaatggtgg ggcgcaacta ttaaaactca aagcaatgcg gggcgtgaaa acgaatcgtg
    26701 gggcgaaagg agaatgagca tgaaacacaa atcattaatg ccaatgaatt tgcaatactt
    26761 tgccgaaggt gacgatcaaa agttttcttt tgatgacttc aaatcttttg tggaatccaa
    26821 tgaagaagct caaaaattta ttcagtcaca atctcaaagt gttgccgaca aacaattgga
    26881 agcttggaaa caaaataact tagataaaat taaacaggat accatcaagg aatatgagga
    26941 atctaagaaa aacaagtcac ctgagcaaat tcaactggaa aaactacaag ctgaatttga
    27001 agcagaaaaa gcgttgcgtg tgacaagtga taataaagca tttgttgcag aacaaattgc
    27061 tggattagaa ctagatggag agttaaaaga gtctatttct caatttatgc taaataatct
    27121 tgttagttcg gatacagatt tcactaagaa tgctgttgaa ggttttacaa gtgtcttgaa
    27181 tgcaattaat gagaaacatg cagatgcatt aaaagaacta caaatgaagt ctgcatttgg
    27241 tggaactcaa caatcgaata accaggttca gcagaacaat gaaacattaa caaatccaga
    27301 agaacaatta ggacaaattc ttcaacaatt taactaggag agtgaaaaat tatgaaaaaa
    27361 acatctttaa ataatttaga gtatttggat atttcaccag cgattaatgc tatgcaagta
    27421 ccaaatacac cttttttaag ctatttattt ggtgctggaa aaacagagcc agcaaactcg
    27481 acagaaatta aatggcgtga atatgatatc aacaacgatg attcttctga aaaacttgag
    27541 ggcggagaat atccagatgc tgaatcaggt cgaacttggt ttaacaacta tactgaaatt
    27601 tttagaaaat caacctctgt atctggtaca ttagatgcta ttaatgtgaa tggagtcgga
    27661 aatgaattaa ctaatcaagt agcactacgt ggtatggaaa tgaaaattga tttgaaccga
    27721 aaattgatta ctggtgtaaa agctgatgaa aatggttcta aaggtcgtcg aatgaatggg
    27781 attttgaact tgatcaattc agcaaataag gcagaaacag ccactgcggg tgcagtaaca
    27841 agaaaagata tcgatacttt atttaaattg atgtatgaaa aaggttatat gggagaaaaa
    27901 ctatgcttga tttctccaga tatgcaggag ttaatgactg atgagttaga tggaaaatca
    27961 acaaaaattg ttcagttcgg ggaaagagta acttttggat tgcaaattgg aaatatcgtg
    28021 tctaattacg gtacaggtat tgctctacta gaaccatcat tgccaaaagg aacaattgcc
    28081 gcaatcgata ctaattatgt gaaactacgt ccattacgtg aatggagagc agaagaactt
    28141 gcaaaaacaa cggattctag acgtatcggt cttgtaggtg aatactctct tgaatacaat
    28201 gcttcaaact ctggggcaat tttaaattta aagtctgaat aaaagggagt tagtactccc
    28261 ttttttgata ggaggaatta cagtggtaaa aaaagatgaa actaaaaaag atgaagtcgt
    28321 gaaatataga gtaggtaaaa ctaaaaattt tgttgggttt gttcatccta aaactcgtag
    28381 atttatcaca gcagattcaa ataacgaatt tatcatttct atagatgata aagaagcaat
    28441 tgcaattttg gaagatgcaa ttgatgttaa tgaaatttag gaagtgatct gatggatgaa
    28501 tcgctaaaaa cggaaatcat tgagtctaca aaagaagatt ttccagattt gagtgaagaa
    28561 cgcataacta atttattaga aataattttg ctagaaattg aatcatacaa cacttgtaaa
    28621 aatgatattt catgggaaaa gttaaaaagc gtgattagtg aagtgttgta taaaataata
    28681 aaaaatgaat cagaaaaaac agtatcttca gttagacgtg gcgatacaac gattagttat
    28741 gcttcaacaa caaatgatgt tagtgagtta cttctaggct atggtgattt gatacgaaga
    28801 gttattggtt gtggaggatt ggagtttttt taatgaatga agcagatatt ttagaaacta
    28861 cttatgaaga tagttgtatt attgaaagac tgacggacat tgaagattct aatacaaata
    28921 ttactattca agattacaaa aaagtatatg ataatcctat ttcttgcgct ctttcacaag
    28981 gtcaaatcga tggactagca gtcatagaag atggagagat ggtaaatgtt tcaactgaca
    29041 catataaatt atttgttcat cctaagatta aactaaaaaa aggagatcga ataacaataa
    29101 ctcaaaaggc cagtggctta attttttctc tatttgctac taagcctttt tactatccta
    29161 gtcattgtga agtgaatttg ataggaagtg agaaaaatgg gtaatctcaa atatgaatca
    29221 aatgcagaaa agatcattga gaattttaaa aatatgactg taattgctca aaaagaagga
    29281 atatccttcg ttaacgattc aatgaataaa gtcgttagtt tagctaaacc attaactcct
    29341 gtaaaatcag gtaatttaag gcgaggatat cgtgtagtaa aagctagaaa actatcaagc
    29401 ggtcgaattg ttggagcagt tataaacaat gaacattatt ttaaatatgt agaagaaggt
    29461 cataggacta aaaatggtgg ctttgtcaaa gggaaattca tgttaactcg tgcaacgaat
    29521 cttgcaaata tgtcttatat tcctcgaaga tttaaacaaa tggcaataaa aatcgttaag
    29581 aaaggaaagt aacatgaaag atgaaatcat tgctgcaatc agtagcaaat taaaagaaat
    29641 ctatcctgat gggacaatat atcttgattc agttatgcag tcaactaaag atttttattt
    29701 cgttttatca gtaatggaat ctggaactga aaatgtagga attgatattc aaaatgtttc
    29761 tttcttaatt gatattgcat tgattgataa taaacctaat agaaatttaa ttaatgaatt
    29821 agtctcacgt tgtgggactt tttttaatac gattacaatc gatgagcaaa tattatttcc
    29881 aaaagcctat ttacctgatg aagcagatgg tgttcaacat attcgtttta cattagaatt
    29941 tccacaatat attgaatgga gtgaaagata aatgggagaa aaaagaagta aaaccggaat
    30001 tatttctgtc gaaaaaccta cttggtttcc tttggaagat gagacaggga attttccggt
    30061 atatggtact gcgttgccaa tgggaacagc agtaagtatc aaaccaacag ctaattatga
    30121 aacaacacaa gattatgggg attcagtggt tcaagatcaa tttacagctt ttggtggtgc
    30181 agaagttgaa ctggaagcaa atggatatac tcataaagtt ttatctacaa ttactggagg
    30241 gaaaattgtt aaaggtggtg ccttgcgttc tggagaagat attgcgcaag atggtgcgtt
    30301 tgcatataga cgtaaaaaat cgaatgggaa ataccgttat acggtctttt ataaaggaca
    30361 gtttgctcta gattcagatg aaacatcaac gattgaagga agtaaagtaa gttttactca
    30421 tccaacttgg aagggttcat ttgtagatgt accaggactt ggatatatgt attcagttga
    30481 tgaagatgat gaaggcgtgg ataaagcaat gatcggaaat tggtttacaa aagtagcaat
    30541 tccaattgaa gaagcagaac tttcaggagg tacagaataa tgtcaaaata tcaaacaacg
    30601 attaaattaa cgaaaaaaga tgaagaaggt aaatatgagc aagtacaatt taaatctgct
    30661 gaatttttac caggaacagt tgtagaagat gctgcaggag ttatggaaga aatgcaaaca
    30721 gcgactgata aacaatcagt taaaaaagct ttaagtcgtg cttattcatt tattgcagat
    30781 actttatttg agggacaatt tactggtgaa gattattgca aaggaattga tgctcgtgag
    30841 attgcctcat tgacaggaaa attattgaag tctgttactg caggttttga tgaaacttat
    30901 acagagacga aaaaaaagta agtgaggcgc tcaagtcacc ttcatttaag tattcgatta
    30961 cttaccgaga attagatata aagacgcaat tactcgaagc aggttggacg ttaccagaaa
    31021 ttgaacgtac tgacttggat gagttaatgc gtctttttgc ttttagagat gcggtaaaag
    31081 agcatgaaga tgttgagtac tatgataatt tcactcaatt ttaggaggtg atactttgaa
    31141 taacgatgac ctaattctga agatgatact agatgaatct ggttttactg ccggtatgaa
    31201 taatgctgtc aaaaagttga actcttttga tgaaacaatc gaaagaaaaa gtagaaatag
    31261 tggcagttca ttaggtagta tttggaaaat atttgctgga agttttctag ctagcggagt
    31321 aactagaatc gtaggagctg gctttgatct aatcaagggt tccataagtg gagcggttga
    31381 tcgagtagat acgatgaata acgctctacg aaatttccaa aatatgggat ttagtaattc
    31441 agaaattatg aagaatatcg gaaagaatgg gcttttatct caaggtattc aaggacttcc
    31501 tactgctttg aatgatgcga taagtcatgt tcaacttctt gcttcttcta caggtgattt
    31561 aactcgttca actcaaatat ttaaagcttt aaatgatgga attcttggtt tcggtggttc
    31621 aactgatcaa gttaatgaag ctgttattca attatctcaa agtttctcaa acggaaaagt
    31681 agacgcacaa acttggaact caatgatcaa cgctcaactt ggacctactc tttctgctat
    31741 tgctaaaaag atgggaatta caatgggaga tctgaaagaa ggtttgtctc aaggtaagat
    31801 ttctgttgaa gaattccaaa atcaattaat agaaatggat accaaaggtg gcggaggact
    31861 taaatcatta agtcaaatcg ctaaagattc tactaaggga attaaaacct ctatacaaaa
    31921 tgcaaaaaca gctgttacac gtggtgttgg tgaggtaata gaaggattaa ataaagcatt
    31981 agtagattca gatttaggtg gatttaaggg aattattgat aaagtgggca gttcaatgga
    32041 atcgttctta aaagtaattg ctgcaaatat tcctacagca gtatcatttt taagtaattt
    32101 atttaacgaa attcaaaaat ttggttctgc tttgaaattc atgatgccct ttcttgttcc
    32161 tgcagctacc gcttttggag cgttcatgtt tcaacttaaa ggtataccag caataataaa
    32221 aagcttcaat aattttaaga atgccataat tggcgttgga aattcattaa agattatggg
    32281 ggcaatagct gctgctaatt catttgtttt aattattggt gcagtcgttg gtgcaatagc
    32341 tgttttcggt tactttatgg caaccaatga agagtttaga aacaaggtta tatctatttg
    32401 gaatgatgtg aaagattccg taattggtgt attaaaaaat ataaaggact ggggaattga
    32461 tacttggaat tctgctaaag aaatggcatc aaatgcagtt gagggtgtca aagatgcttg
    32521 gtcaggaaca aaggaatggt tttctaacac ctggaaaggt attaaggatg gggccacagg
    32581 tttatttgat aaaacagtag agacttcaag aaatgcagtc gatagtgtaa aaaatgcatg
    32641 gtctaatatg aagaaatggt tttctgatac ttggcaaagt ataaaagatt cagccagaga
    32701 aaaatggaat gaaattaaag gttctattat gtcggttgct ggtccattga ttacaggtat
    32761 aaagaatgca tttttacatg ttacttttta tctagagacc ttatggaata atttagttga
    32821 gattggtaaa aacgtctttg agattttaaa aaatgttata cttgcgccgg ttttgtttat
    32881 tacctccatg atttctggtg gatgggaaga aacaaaaaat aatatgattg ctgtttggaa
    32941 taatattaaa gaaagtgcca taaatatttg ggaatctata aagaatatat ttgtaagtta
    33001 ttttacaaat atttattttg ctgcactcaa tatttggaca gggttcaagc ttacattgat
    33061 aaatatttgg aatgaagtgg taaatcaagc taaatcgatt tggttaaaca taaagtattt
    33121 ttttattaat ctttggattg atataaaata ttttgcaatt cagaaatgga ttgaattgaa
    33181 gttttct
//
LOCUS       DAFGZE010000029        32822 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.029, whole genome shotgun sequence.
ACCESSION   DAFGZE010000029 DAFGZE010000000
VERSION     DAFGZE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.029"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(55..1533)
                     /locus_tag="LTU54_001428"
     CDS             complement(55..1533)
                     /locus_tag="LTU54_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296150.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1830..2765)
                     /gene="murB"
                     /locus_tag="LTU54_001429"
     CDS             complement(1830..2765)
                     /gene="murB"
                     /locus_tag="LTU54_001429"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBL8296151.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2838..3758)
                     /locus_tag="LTU54_001430"
     CDS             complement(2838..3758)
                     /locus_tag="LTU54_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8296152.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3871..4623
                     /gene="xth"
                     /locus_tag="LTU54_001431"
     CDS             3871..4623
                     /gene="xth"
                     /locus_tag="LTU54_001431"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBL8296153.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5487..6020
                     /locus_tag="LTU54_001432"
     CDS             5487..6020
                     /locus_tag="LTU54_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBL8296154.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6510..7034)
                     /locus_tag="LTU54_001433"
     CDS             complement(6510..7034)
                     /locus_tag="LTU54_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296155.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(7027..7500)
                     /gene="tsaE"
                     /locus_tag="LTU54_001434"
     CDS             complement(7027..7500)
                     /gene="tsaE"
                     /locus_tag="LTU54_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBL8296156.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7623..8603)
                     /gene="pta"
                     /locus_tag="LTU54_001435"
     CDS             complement(7623..8603)
                     /gene="pta"
                     /locus_tag="LTU54_001435"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBL8296157.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8756..9436)
                     /locus_tag="LTU54_001436"
     CDS             complement(8756..9436)
                     /locus_tag="LTU54_001436"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL8296158.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9562..10431
                     /locus_tag="LTU54_001437"
     CDS             9562..10431
                     /locus_tag="LTU54_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8296159.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10457..10972
                     /locus_tag="LTU54_001438"
     CDS             10457..10972
                     /locus_tag="LTU54_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296160.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(11019..11612)
                     /locus_tag="LTU54_001439"
     CDS             complement(11019..11612)
                     /locus_tag="LTU54_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296161.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11637..11816)
                     /locus_tag="LTU54_001440"
     CDS             complement(11637..11816)
                     /locus_tag="LTU54_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296162.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11893..12402)
                     /locus_tag="LTU54_001441"
     CDS             complement(11893..12402)
                     /locus_tag="LTU54_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296163.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12498..12878)
                     /locus_tag="LTU54_001442"
     CDS             complement(12498..12878)
                     /locus_tag="LTU54_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8296164.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13208..13957
                     /locus_tag="LTU54_001443"
     CDS             13208..13957
                     /locus_tag="LTU54_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBL8296165.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14141..15418)
                     /locus_tag="LTU54_001444"
     CDS             complement(14141..15418)
                     /locus_tag="LTU54_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296166.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15497..16141)
                     /gene="pcp"
                     /locus_tag="LTU54_001445"
     CDS             complement(15497..16141)
                     /gene="pcp"
                     /locus_tag="LTU54_001445"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL8296167.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16340..17284)
                     /locus_tag="LTU54_001446"
     CDS             complement(16340..17284)
                     /locus_tag="LTU54_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL8296168.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17286..17972)
                     /locus_tag="LTU54_001447"
     CDS             complement(17286..17972)
                     /locus_tag="LTU54_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL8296169.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            18446..18766
                     /locus_tag="LTU54_001448"
     CDS             18446..18766
                     /locus_tag="LTU54_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL8296170.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            18920..19825
                     /locus_tag="LTU54_001449"
     CDS             18920..19825
                     /locus_tag="LTU54_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8296171.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            19822..20595
                     /locus_tag="LTU54_001450"
     CDS             19822..20595
                     /locus_tag="LTU54_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL8296172.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(20776..22200)
                     /locus_tag="LTU54_001451"
     CDS             complement(20776..22200)
                     /locus_tag="LTU54_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296173.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            22411..22857
                     /gene="nrdI"
                     /locus_tag="LTU54_001452"
     CDS             22411..22857
                     /gene="nrdI"
                     /locus_tag="LTU54_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL8296174.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(22922..24904)
                     /locus_tag="LTU54_001453"
     CDS             complement(22922..24904)
                     /locus_tag="LTU54_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8296175.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(24918..25814)
                     /gene="murQ"
                     /locus_tag="LTU54_001454"
     CDS             complement(24918..25814)
                     /gene="murQ"
                     /locus_tag="LTU54_001454"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL8296176.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(25817..26878)
                     /locus_tag="LTU54_001455"
     CDS             complement(25817..26878)
                     /locus_tag="LTU54_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL8296177.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(27089..27655)
                     /locus_tag="LTU54_001456"
     CDS             complement(27089..27655)
                     /locus_tag="LTU54_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL8296178.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(27683..29185)
                     /gene="eat(A)"
                     /locus_tag="LTU54_001457"
     CDS             complement(27683..29185)
                     /gene="eat(A)"
                     /locus_tag="LTU54_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL8296179.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(29443..30564)
                     /locus_tag="LTU54_001458"
     CDS             complement(29443..30564)
                     /locus_tag="LTU54_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL8296180.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            30690..31631
                     /locus_tag="LTU54_001459"
     CDS             30690..31631
                     /locus_tag="LTU54_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL8296181.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(31781..32677)
                     /locus_tag="LTU54_001460"
     CDS             complement(31781..32677)
                     /locus_tag="LTU54_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL8296182.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
BASE COUNT         9562 a         6798 c         5741 g        10721 t
ORIGIN      
        1 aaaaaacata aatcagctaa taaatatgtg agacaaagct gccatccata aatactagct
       61 gattttttca gttaaatgtc tatttttttc tctgatgatg gtatttccaa aatattccca
      121 gtcatctgga gtcggtggat ttttccaaat tatctgttct ttattaaatt tttcaagtaa
      181 acaatcagat aaataaatat tcgtacgaga agtgattcga ctctcaatca caatattgta
      241 atttttaaat cctttcactt gagaaataat gtaatcggta taagcagatc cttgcgaaaa
      301 atcaatacag acatgaatcg gtgcttcaaa atatgatacc ggacaaatac cgaccaataa
      361 aaagagataa tcataaaata agcgaatcat gaaacgttca tcttttccca agaaatagcg
      421 tttattctct tgtatgacct tttggattgc tgtactgaat attggatacg tctcgaagaa
      481 aaactgtagc tgctttttag acgtaaagct agtcatctca aagtcgaata tccaattttt
      541 tcgattgaca tgcatcatac ccatacgaag ttcttttcgt atatatagtc tttcttcttc
      601 agagatttca atcgaaaatg gtaattgatc aatcaattcc actgacattt tttgactaat
      661 catgtggata tcatgaaaat gagcttccgc aagtccaaat gcgccatcta attcaaattc
      721 ttcttccgtg atcaaaaaca acatgatata agaaatctca tcaatgatct gctctttagg
      781 gatatgaaga aaagaaatat gtgataaatc cttgatgatc ttcattcctt ctgagctctt
      841 aaagtattca tctgaaaaga aacgttttcc gattggttga ttcccatagg ccctcaacaa
      901 actaatagtg gttagcaaat tgaatttgat tcgttttgtt gcagacagtt ttaaattgaa
      961 cagatattca aaatattgca cgatactctt tgcttcattc atgacgcttt ctctaaatgg
     1021 catagaaaaa ccattaaaga ccccgaaata taccccaaat aatacgttcc gtatgtttat
     1081 ttcttcccca actaatatgt ttttctttaa ctttatttga tgctgttcaa gaaaagagac
     1141 taattctctt ctttttacat aggctcttga tatgcttaaa aaatgattct ctgcaaattt
     1201 ttcgatcgtt acttttgaat ccacgatctc cgttaacagt cgagcaattt ttgatcgttg
     1261 aaagtagatt cttcttaaat ttttgatatc cgtcaaattc agacgttcga catggatctc
     1321 tgcttcttct gaaatttgga tctctggctt tgttttgaat tcagtaaatt ctttatttaa
     1381 ttctatgata taatttcttg ttttaaattt agacaaacct aatgtttcat ataacttagg
     1441 cagcactaca taattctttt tctcaaattc taaatatcta gctagcagca gtttttctct
     1501 atcttcctct tctataaggt aatccaactt catatcctac acctcaaaaa atgcctcata
     1561 ccaattactt gatatgcaga tgaaagttca tcttttcacc tatactgatt ttatgatgga
     1621 ttatgcttgt tttttcatca gttattctca ttttttatct ttttttgaga atattgcaaa
     1681 aaatggaaaa atttatgagg aaatattcta tattttttgt tttattgtgt taaaatgtac
     1741 aagatttgcg ccatttgcgt aaacaaccac aaaaaagagc aggacggcca ccgtcctgct
     1801 cttttactta ttttaagttc cattacttat taattggcta aacttctgct tgtttctgat
     1861 attgcttgtt tttctccaat gatccgcact tctgtttcta aagacacatc aaatttttct
     1921 ttgattactt cttggatgtg cgcaatcaat tcaatataat ctgtcgctgt cgcatgatcg
     1981 atattgacga tgaatccagc atgtttttca gagacttgtg caccgcccca tttcaggcct
     2041 tgcaatcctg cttgttggat cagctgacct gtatagtgtc ctactgggcg cttaaagaca
     2101 cttccgcatg aagggtattc taatggctgt tttgattgac gcaattcggt caactcatcc
     2161 attcttgctt tgattgcttc ataatcccct gtttgcaaac tgaatcgagc ttccaatacg
     2221 atagcacgta attcttggac tttactgtga cgataagaaa ataccatttc gtcttttgtc
     2281 aaagttttca atgttccatc ttcaagcaaa agatccactt cagcaaaaac atctttgatc
     2341 tctccgccat acgcacctgc attcatataa accgctccac cgacacttcc cgggatgccg
     2401 caagcaaatt caaatccagt caatgattct gccaaagctg cataagtcgt atcaatcagt
     2461 gaggcacctg cttccgcaat gaccatcgtt cctttgacat gaatctgttt catttcagtc
     2521 agcatgatga ctacaccgcg aatcccgcca tcttgaacga tcagattgct tgcatttcct
     2581 aataccatcc aaggtatatc atggattcgg caataatcga tgatttgctt tacttcttct
     2641 tgttttttcg ggaatgcaag gacatcagct ggtccacctg ttttcgtaaa cgtatagttc
     2701 atcaatggtt catcaaacaa tatagtaatc gtggctaagc tttcaactat ttcttcttta
     2761 ttcactgagg attttccttt cttttctcaa acgtcctcat tattctatca cgcttaatac
     2821 aatcttgcca gtcttgttca aagttgatga cggtagtgat ctttaagcac ttcctcacat
     2881 aattcatgac ttcttacaac ttctttttgt ttaagcaatt ctgtttccga tttacctaaa
     2941 cggatctgag aaaagaaggc ctctaccatt ggctcaaacc cgcgtttgac tagcgtgttt
     3001 gtccagtcgc caaattcttt cacttttttt ccatcagcat cttgaatgac aagctgggtc
     3061 agatcgttta cttcataagt accattttta cttgttactt gatatttttc tatattagcg
     3121 ccacttgcta aatccatcgt taaaatagcc gtttgatttt gtgtttctaa ttgtaaaatg
     3181 gcatattcca aaaattcctt tgtttctctt atctggtatt ttgttctcaa aatgggttca
     3241 tctaataaat acacagctgt atcaataaga tgtaaaaaca aatcataaag gacgaactct
     3301 gtcgtttctt ttgccgctat tcgatttttt tgcaggtgga tcaaccgctt atctgggata
     3361 cgtttcaatt cttctaccat aggagcaaag cgccggttga aaccgatcat caaaattatt
     3421 tggttcttat cagctaattc ttgcaattca cgaacttcat ttagatggac actcagtggt
     3481 ttgtcaatat aaacatggat accattttct agacattttt tggctaattc ataatgtact
     3541 tttgttgctg cgtggatcat acatgcttcg attttttctt ctaaaagctc atcgatcgtt
     3601 tcatatagat gttggaaccc gtactctttt tgaattcttt cttttgtttc tttatttctt
     3661 gttgcaaaat aaaaatcaag tgttccttgt tttttcgcat aagttggtaa atatgctttt
     3721 tctgcgatat tccctacacc gattacacca atcttcatct actttcctcc tggatcattt
     3781 agttactttc atcttacacc attttttact aaaatgaggt acaatagtat cgcaaaggta
     3841 aggaaattaa ttcgaaggag tgtgctttta ttgaaattta tctcttggaa cgtaaacggg
     3901 cttcgtgcca tcgtaaataa aaatttttta gaagtttttc atgagttaga cgccgatttt
     3961 ttctgtctgc aggaaacgaa actccaagca ggacaaattg atttagatct tccaggttat
     4021 tatcaatatt ggaattacgc ggaacgaaaa ggctattccg gcacagcgat ttttgctaaa
     4081 aaaccagcat tgaatgccac ttacggaatg ggtatcgata tccatgatac agaaggccgt
     4141 ctgattactt tggaatattc cgatttcttt cttgtaacct gttacacgcc aaattctcaa
     4201 agcgaattaa aacgtttaga ttaccgatta gaatgggaag aagcctttta taattatttg
     4261 gaaaatctaa aaaaacaaaa accagtcatc gtgtgtgggg atctaaatgt cgctcatcaa
     4321 aagattgatt tgaaaaactg gaaaacgaat caaaaaaatg ctggattcac tccagaagaa
     4381 agagcggctc tttctcgact gttggataac gggtttatcg atacattccg ttatttttat
     4441 ccaacccagg aaggtgtcta ctcttggtgg aactaccgat tcaattcaag gaaaaacaat
     4501 gctggttggc ggatcgatta ctttttgacg agcaaagatt tagagccagg actagctgat
     4561 gcaaagatcc atacggacat tatgggcagt gatcattgtc cagtagaatt agatttgaaa
     4621 taaagaggtt gtgaaaaagt cgtttagttt cgagtattaa gaaaaatttt gtgaaaatgg
     4681 ctttccacat tttgcataaa tttggcataa taccgagaaa ctggcttttg aacactgttt
     4741 atcgagagat tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata
     4801 gttcctcata tttttcgttt ggttgggctt attaccgcag atacaagctt ttgaacaccg
     4861 tttattaaga ggttgtgaaa aagctgcact gcatcaagta ataagaacag ccaaacaaaa
     4921 atagttcctc atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca
     4981 ccgtttatta agaggttgtg aaaaagctgc actgcatcaa gtaataagaa cagccaaaca
     5041 aaaatagttc ctcatatttt tcgtttggtt ggacttatta ccgcagatgc aagcttttga
     5101 acaccgttta ttgagaagtt gtgaaatcag cgtcctgacc tgagtattag agaaacaaat
     5161 ggcaaaatag ttcctcatat tttgctattt tttgagctaa taccgagggt cagctgattg
     5221 aacaccgttt agtggagaag ttgtgaaaaa gtcgcctact ttcgagtcgt aagaaatttt
     5281 tttgaaaatg gttcctcaca actttgcata aatttgactt aataccgaga agtcatgatc
     5341 cccatctaac tccaaatcac aaggaatgag ttcaaaaact agcttttctc aacaaatatt
     5401 acataaaaaa ttctatgttt tttgttaaat ttatttgctt ttttcaaaag tatgttacag
     5461 taatttccca aggaggtttt acagaaatga attttgttgc catggatttt gaaactgcca
     5521 atcatcagcc ttatagtgct tgttctcttg cgctagtcat ggtaaaaaat agccagatcg
     5581 ttgatgaatt ctatacattg atacagcctg agactccttt tttctggcga aatgtccaaa
     5641 ttcacggaat ccatcaagaa gacgtacgca atgcgccgaa attcccagaa gtctggcaaa
     5701 ctatccaacc atattttcaa aaaaatcgtt tagtcgttgc acataatgct gcttttgata
     5761 caaaggtctt agcgggatgc ttagattatt atcatcttga acagcctaat tatctttctt
     5821 tatgtacagt gaaaagtagc cgtcgcctgt ttcctgaatt tccaaatcat cgattgaaca
     5881 ctgtgtgcga aaatttgaac atccaattag atcatcacca tgatgcattg gaagatagtc
     5941 gtgcatgtgc ggaaatcctg ctttatcaag aaaaaaaatt cgggacagat ccattgaaaa
     6001 aacttgtgac tataaagtaa aagaatactt atgaaacaca caaagaggtc gtgagcctgc
     6061 cgtttcactc cgagtcacaa ggagcaattt caaaaaatag cttctcatgt ttattaaata
     6121 ttgtgactgg tgaccgagga gctggcagga gaacaccgtt tattggagag gttgtgaaaa
     6181 agctgttctg catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
     6241 ttggttggac ttattaccgc agatgcaagc ttttggacac cgtttaccaa gaggttgtga
     6301 aatcagcgtt ttgacctgag tattagagaa acaaacggca aaatagttcc tcatattttg
     6361 ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttattc aaaaaaaatg
     6421 gactgcgaca cgacttttgt cacagtccct tcgtgtgttt ttctgccttc tcaaaaggat
     6481 gacagtttct cactcttttc ctatttcttc tattcaatca aataactcat tttatataca
     6541 tctaaaaatt cacctagatc tgttcttgcc cctctaggca tgaccgcttc gatttggaag
     6601 cccatttttc ggtaaaggtg tacggctctt tcattacgaa cttgaacgtc aagctccaat
     6661 cgaaaaagta cacccatttc cttcgcccaa ctgatgattt cttctagcat aagagtgcct
     6721 agtcccattc cccaatattc ctgcagaata ctgataccga cttctcccac gtgagacaaa
     6781 cgaaattgac tgtctgcctt gacagatgct gttccaataa tcgtcccttc atagattgca
     6841 agcagcaata aattattatt gctttcgtat atataatcca actcttcttc taaggtttct
     6901 ggtggtaaca ggagttctgc ttcatccaaa actaaaaatt ctgtctcctt attaacttgt
     6961 cccatcatca aaagaatgtc ttttgcatct cctggcactg cttcccgcaa gatgatccca
     7021 acttcttcac tcatgatttt cagcccattt ttctagaatt cgctgtttga aggtttcact
     7081 ttgctccccg tatgagatta gtttcacata ccgcttttct aaatctgtat cacttttttc
     7141 taaaatcagt tccaaataat cctccggcag tgcttcttct aatagatttc cccattcaac
     7201 aacggaaagc ccatcacctt caaaatattc atctaaccct aaatctgctc cgctagctgc
     7261 tacccgataa atatccatat gatacaacgg aatccgacct tgactatatt ctctaataat
     7321 cgtgtatgtc ggacttttga tcatttgcgt tatccctaat ccttgggcaa ttcctttcgt
     7381 caacgttgtt ttacccgcac ctagatcacc agtcaatatc agattgtcac ctggcattgc
     7441 ggaatgacca attgcctctg cgaattgttc tgtcattgtt aatcctgata gttcgatcat
     7501 ttttcatctt ctcccgctct tctttctttt tgccagtata tcgcgtaatg aagttttttt
     7561 aaagaaaaaa tgaactagtc aagaacagca gctgctgttt ttggctagtt cgcgtgtatt
     7621 tgttaatcaa tcaatgtttg tgctgctgtg ataatcgata atttatatac atcttcttca
     7681 tttgctccac gagacaaatc agaaaccggt ttgttcaagc cttgtagaat tggtccgatt
     7741 gcttcaaagt taccgaaacg ctgagcaatt ttatagccga tatttcctga ctgcaattct
     7801 ggaaatacaa agactgttgc ttgaccagct acttctgaat ctggcgcttt cagttgtgca
     7861 actgaaggaa cataagaagc atcaaattgc aattcgccgt cgatcatgta ttgtggcgct
     7921 agttttttag cgattttagt tgcttcaact actttgtcta cttctggcga tttcgctgag
     7981 ccttttgtcg agaagctcat cattgctact tttggttcga tatcgaacaa ttctgctgtt
     8041 tttgcagaat ctacggcgat ttcagccaat tcttgcgcag ttggattcac gttgatcgca
     8101 caatccgaaa agacatattt ttcttgatct cttccacgta ccatgatcat cgctccgctt
     8161 gtccgactga tgccaggctt tgttttgatg atctgaagtg ctgggcgaac agtgtcgcca
     8221 gtcgaatgga tcgctccaga gaccattccg tctgcaatcc ccatatacgt aagcatcgta
     8281 ccaaagtaat tcacatcttt taaaatcttt tctgcttgtt ctttggatgc ctttccattt
     8341 ctacgttcaa caaaagtatc caccatttca tcccacttat cgtaattatc agggctgatg
     8401 atcgtaaagc tagatgtttt gattcctctg gcatgcgctg catttcttat ttcttcttcg
     8461 ttccctacta aaatgggttc cacaagttct tctgctttca accgggcagc tgcgcctaaa
     8521 atacgagcat cagtagcttc cgggaaaacg attttgatcc cacggcgaac aattttaaat
     8581 cttaaactat caaacaattc cacaagatga cctccagacg tttttatatg tattatcata
     8641 cactattcca gaaaaaaaga acagtttatt ttatctgtat cagtcaaaaa tgctggaaaa
     8701 attaagtttt tcacatacaa agcgaactgc actataacag gaaagcgctg ttttattaca
     8761 ctgtttcagg cagttgccaa tcaatcggtt cttctcctaa cgcaaggagc gcatcattcg
     8821 ttttcgagaa gggtttggaa ccaaaaaagc cacgacttgc agataacgga ctgggatgtg
     8881 gagatgttat gatgatatgg cgactcttat cgatcatttt gattttttct tgtgccggtt
     8941 ttccccacaa gataaacacg attggttttt cccgttcatt taatttttca atgatcgtgt
     9001 ctgttaatct ttcccaacct ttcccgcgat gagaatacgc ttgcccttcc cgaacggtaa
     9061 gaacggtgtt gagtaaaagg actccctgtt tagcccatga cactagattt ccatgttgaa
     9121 caggtgcgat tccaagatca tcataaagct ctttatagat atttcttaac gatggcggga
     9181 tctttacgcc aggttgcaca gaaaaagata agccatgagc ttgattttct ccatgataag
     9241 gatcttgtcc taaaatcacc actttaactt cttcataagg agtcaattcc aatgcttcat
     9301 agatatgata catatctgga tggatctttt gggacgcata ttcttttttt aaaaattctc
     9361 ttagatgcag ataataatct tttgaaaatt ctgaggacaa aacttcttgc caactgttat
     9421 gaatgatttc tttcaaaaac tccacctcct aataaaaaga ataactcaca ttttcttcaa
     9481 tcacaaggga caatcttcac cctcagcact tgattaatat ggtaaactag gaacaagaag
     9541 cagaaaggaa cgataatcat tatgatcaaa ttaattgcat cagatatgga cggcacatta
     9601 ttagatgccc atatgagtgt ttctcctgaa aatgccgagg cgatacgttt tgcaaatgat
     9661 gccggtgttg agttcatggt cgctactggt cgaaactatc aagaagctcg cgcagcttta
     9721 gatgaggtag gaatcgattg tgcgatgatc actttgaacg gggcacaggt atttgataaa
     9781 gaaggaaatt cattgtttac cgtgtcgatt ccaaataatc aagcaatctc tgttttagat
     9841 attctagatg ctaacggtat ttactatgaa gtagcaacca acgatggtct ttattctgaa
     9901 aatcaagcga aacgaattga aagttttgct tccatggtgg ctacccattt acctcacctt
     9961 acgtataaaa tggcgattgc catggcctca gctaagctgg agttattgca catcacatac
    10021 gtggacagta ttcgtgctat tttggaagaa aagaatctgg aagttttaaa aattatttgt
    10081 tttcatacag aagggccagc tgtattaggt ccagttggaa agcagatttc tcttttaggc
    10141 gatctagccg ttacttcctc tggtcaaaac aatatcgaag tgaaccataa aaacgctcaa
    10201 aaaggaattg ctgttgccca tgtcgctcac gagcgtggga tttctttaga agaagtcatg
    10261 acgattggcg ataacttcaa tgatgtcagc atgctgcaag tagcaggagt cagtttcgca
    10321 atgggaaatg cagaaatcga agtaaaagat tatgccaaat atacaacgga cacaaacctt
    10381 gaatctggcg taggaaaagc catattgcgc gcgattgcag aagacttgta gttttatcta
    10441 gaaaagaggg atatagatgt cagaattttt cattcaggaa aagcagcttg gacgtgcaac
    10501 tcggacaatt gtcaaaaacg aaaaaggaca ttccctgttt ttgatggttg gtcgctgggg
    10561 aacaaagggc gatgcattgt ctctttatgc catgaatggc gatctcgttg ctcacatcaa
    10621 gcagataagc ctgacattcg gtacgcgttt tgagctgtat aaagagttcg aaaaggtggg
    10681 aaccatgcag aaaatattca attggcccgg tgacttttat tatattcgta gacttcactg
    10741 gacagtccaa ggtgatatct ataatcatca atatcaaatc catcatttca atcagcaaat
    10801 catgaaaatg gataaagcaa cacttttttc aggagattac tatgtattgg atgtctcaaa
    10861 tgatgaagat gctccagttt gtatttgtat tgctgcggtt ttagattact ggctctaccg
    10921 taaagacaaa agtcgcgata agcctgtttc ttaccgactt aacttaagtt aaaaagatgt
    10981 acctgaaatg cggaaatacg catttcaggt acatcttttt agatattctt ttgcatatga
    11041 tcataaagat gaccactgat catccgctga ccgacaactt tgaagtcatg gcgttcgtat
    11101 aattttttag cctttggatt tttttcatcc acacttagac cgatgacgga gcgattagct
    11161 ttcttcgcca atttaggtaa tgcttctaat aatcgcgaac cgatgccttg tcctctaaaa
    11221 tcttctgata cactgataga gtccaaatac cattcgttgg gaaacgtttc tttatcaatg
    11281 aacaaacgtg tagacgcatc tagctgatat ttctctaaga taggtaccag tggctgatcg
    11341 atgattgatt cttcttcatc tgtataaccg taagcaactc ctgcaacttt accgtctatt
    11401 tcttctacga tacctcgtga ataactgtaa cgataatttg gatcagtgat tgcttcttct
    11461 aaaatctcta aagtcttttg ttcccctgtc tgcttcaaaa atggcagttc catatctttt
    11521 agaatcacta agatcaatgg ggcgatttct ggagcatctt cttttctcgc aaaacgaatc
    11581 atagtttcct ctccttttct ccattctaac atgcagaaaa agaaatcgca aaatcgttat
    11641 tttaaaaaat cttcaatctt ttcagtaatg aaataaccat gagtaacgat tcttgccgct
    11701 tcagttcggc aataagggca gacgactggc atttctttat cattatgttc gactacatta
    11761 ttgacttctt cgtatgcaaa tttttttccg caattagggc atttcatttt ttccatgtta
    11821 tctcaacttc ctttcatctt tactctctta gcttactctt atattgtaaa aaagagaagc
    11881 aatatccttc ttctatgcag atatccatac ctgacggtcc tgttctccat ctattaatcg
    11941 gactttataa taaggacgtt catctttgac tagaagtgtc cactctttca cttttgcttt
    12001 tttcccgtag tagctgactg cttccttcat cgctgcttgc ggttgtttga actcttccgt
    12061 tttttctata tcaaaaagat gttctgcggc cttttccgtg tcatttcgta cggttgtttt
    12121 cccagtggat ggattgactg ctatgacttg tccttcacaa aaaaactggt attcatatgt
    12181 tgcattcgta ggattctcca aatctttttc aactgcaaaa tgaatagcag atatgttatg
    12241 ttcttttgtt tcgtttttta cgattgctgc tgcttttttc aatgagatcc gatattcctg
    12301 gtcactaata tcatccatgt gaaaagcggc attgagcagt cccgtcatac cggtaatccc
    12361 tattacgatg agggcagcaa caccaaacat catttttttc attcttcatt ctcctgtttc
    12421 ttttttttaa agaatatcaa gaaatgaacg aatgaaaaat cgaatgaaac gaaaaaaaag
    12481 tgacaatttt gttatcttta ttcgtgcagt tctaatggca atccgtctgg atcaaagaaa
    12541 aacgtcattt tttttcctgt aaaggtatct tctctaatcg gttctgattc gatccctaag
    12601 gactgcaatt ctgcaattgt ttcttcaata tcgtctactt tgaaagcaag atgacgcaag
    12661 ccacaagctt ctggatagtt tggtcgttgt ggcgcattag caattccaaa aatctccaat
    12721 tcagcacttc ctagtttcag atctaattta tagtcatctc gttctttgcg gtaattttct
    12781 ctgattactt caaatcctaa tttattaaca taaaaatcac gtgatttctg ataatctgaa
    12841 acaatgatgg ctatatgatg gatcgttgta aggttcatat gagcactcct attcttcgtt
    12901 taagctcgga tctatattaa tctccagttc cattgtaaat gtgtcagatt gaaaaaaata
    12961 gctgtttttc ctttttataa gaaaaaaggc aaaaaaaaca gaaagtaggt cctccaagac
    13021 actcttcctg ttcccaatat aataaatgaa agtaagaaat tatattttca agaaaacaaa
    13081 cagaaaatta tcataacaca aattatttat aattgaaaga ctagtttttt ttcaagaaaa
    13141 gcatacaata gattgtgtta gtaaatacag aacacactac atttagtgtc ttaaggagtc
    13201 ctcatacatg aagtcatatt ttcagttttt agctcatcag ctaaaaggct ggccgcaaca
    13261 aaattattat cttttctttt tcagtcttgg ctgccaggtc atgactcttg tcaatagtcc
    13321 catcacttgg ctgtccgtta ttacatttat cggtaccacg cttggtgtat tgtgtatttt
    13381 atccatcaat gcggcaaaat cagtcaacgg tattttaggt attttgtctg cgatttgttt
    13441 tattatcgtt gggttttcag caaagaacta tttaagtatc ggtgaacaga ttgcttatat
    13501 gatcacattg gatatccccg tattgttaag taaagaatgg aatcataata tggcttcaaa
    13561 aatccgaaaa ttcactggaa aaagctggtt tatcgcaatc atcagtactt tccttgtcta
    13621 tcttgtttca ggattcctga ttggtcgtta tacagatgat cctcggccgt gggtagatgc
    13681 cattgctttt tctattagct tgactgctgg tatcatcagt ttcttgcgtt acaacaacca
    13741 atattactgg tggttagctt cagggcttgc tcaaatggtc ctttggtata tttcatttac
    13801 gcatggatct gcaagtttgg cgatgttcgt gaacagctct gtttacttgc tgaatgatgt
    13861 ccttgcattt accgtttcac cttggtataa ccaaaaagaa cgtgaacgta tgcaaaaaat
    13921 ggaagaagaa tactacaacg cacaacaaca cgcataaaaa aactgagcgt gggacaaaag
    13981 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaatca gaagcaactc
    14041 cttcggaaat aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt
    14101 atttcttgga gttaaacgct tctgtcccag cctctatttt ttatagtaat tccttcagat
    14161 actttcttgc ctcatcattg aaacgcagtg attcttctaa cttctgataa tattccaacg
    14221 cttgtttctg agcttcagta aaacgataat gttttccttt ccatttgtcg attgtctctt
    14281 gcacatttcc agattggata tctttcacta ctagttgcca tttttctttt gagactgtac
    14341 ggtctttctt atcagcagat tgtatagctt ctcgttcttt acgcaatact tttaaaaaac
    14401 catttctgac tatccgagga atgctgtcga agaacccttc tgtaaaacca tagcgacaat
    14461 attgcttcca aataaataga tagatttttg gttggatgaa tcgttggttt ttcgcaaaat
    14521 attccatctg agcagtgact tctggtgttc gttgacgttt tgtctttctc aacacggaaa
    14581 taaatgtttc aaaataagca ttaggatgta ctggcacttc ttcatatacg atggattgcc
    14641 aatctttacg taaaaatgcg cgatagttga tcaagaattc ttctacatcc tttactaatt
    14701 cttctgcaaa gtcgtcaaat agttcttttt ttatctgtcg cttttttgac tgcccattga
    14761 tgtgttcact tattttcttg atttcatttt ccaatgcttg ataatccgct atgtaaatcg
    14821 gcatttctac ttggcgaaat tctgcgagcc gctgagctaa ttttatatcc ggtaaatatg
    14881 gctgtttcat ccgacgattt tggtacaaga caaaacagta tttttgccat agtccttcag
    14941 gaaaatcgat attcttctgc ttaagccata ataattcgtc taaggccaga tcaacttttg
    15001 tcattccctg atggatcgaa gctgccactg tcggagagac atgcaggtga tcggcaaagt
    15061 ggttgatgcc tagttttagg atctttccgg tttttggcga tttaacgatg tattgcactt
    15121 ttaattctct accacactca caatacaatt tttgctggct tcgttttgtt cgaaaaaacg
    15181 gattgatctt gaagtcagaa aacacccaat ctgttgcagc taaataatgt tcttccataa
    15241 aacgagagct gattagatat cggcggtgct tatcgactag ttcccgctgt tttttcgtta
    15301 attttgcata gatctcccaa cgttgactgc ttgtgtattg tttagaaatt ttgatgggat
    15361 agcttttgct gatttctttt ggaaaccatt cttcaaagac ggattgccat tcttccacgg
    15421 ctgtttcttt cctttctaat tacaaaaagc tggctactaa cataacgtta gcaaccagcc
    15481 agtgtttctt gctaaattag tgagttgttc cggcatttcc ggaaatatct tctttatttt
    15541 ctactgctgc ttcaactgca taagtcaaag aagaagcaat gtcttctaaa gacatagaag
    15601 gcgtacccac tggacgatcg atcacttgat cgggtgagaa tggcacgtgg atgaacccac
    15661 ctcgaattgc cgggaattgt gtttcgatca aatgaagcaa acgatacatg atatcattgc
    15721 agacaaatgt tcctgctgta taagagatgt acgctggtag accatgatct tgtacatttt
    15781 ttgtcattgc tttgattggc aatgaggtaa agtaagcggc tggaccgttt tcttctaaag
    15841 ccgtatcgaa tggctgattt ccttcattat cagggattct tgcttctgct agattgattg
    15901 cgacacgttc gaacgagact gctgaccgcc cacctgcttg tccgacacag atcacgacat
    15961 ctggttggtg ctgttggatg gcttctttga ctacttgact gcttttttcg aatacggtcg
    16021 gtacttccac tttgatgatt tcagcaccgc tgatttcatc tggcattttt tttactgctt
    16081 cataggctgg attcactttg tccccaccaa atggatcaaa acctgttact agtactttca
    16141 tctgttgttc ctccgtccga ttgatttaac atatggttat cttttatcat accattttcc
    16201 ttgaacagcg taaataaagg aaacaatagt ttaaataaca taacagatca aacttaatat
    16261 attcttataa aaaaatagtg ttccactaca atgttagaag aggttgcgac aattattctg
    16321 tcacaacccc tctcttctat tatttaaata gaatcatata agcaatttgg aaaactagca
    16381 taaataacgc aataaacaac tggtttttga tcactccgta tttgtctttc atctccagca
    16441 tcgctacagg cacgatattg aagttagcag ccatcggtgt cagcagggtc ccacaaaaac
    16501 cgcaagtcaa tgcaaccatt cctattaacg ctggattcgc tccatgacta aatacgaatg
    16561 gtgcaccgat cccaaccgtc atcacggtga tggccgcaaa tgcattcccc atgattaccg
    16621 taaatatcac catgccaaga gcgtaaatga tgatgccgat gttcacattt ccttctggaa
    16681 cgatcgtacc tactgcattg gaaatcactg tcccaactcc agcgcttgta aataccgcac
    16741 ctagagaagc taaaagcatt ggcaacatgc ttaacggccc tactgtgccc agcatatctg
    16801 ctgcatcatc taaaaatgta cttggtcgat tatctcgaga aaagaacatc aagattagca
    16861 tggctgcaaa aacaccgacg cccacgccga ctaaagcacc taaattggta aatagagcaa
    16921 acaagatcgc aaagacaccg atactcaaag ctggaataaa aattttcatt cctagtttgt
    16981 ctgacatttt ttccatatat tcttttgatg gaagccggct ttctcctttg ctgactttct
    17041 tagcaattgc tggtatggtc atcgcaaaga tcaagccacc gttcacgatt gccggaagcc
    17101 atcttcctcc tgcaataact acccctaaga ccgtccagaa aaaggctgac ccataacgat
    17161 gaggattggt tttatctgtt aaatttctta ctccagtgta aatcaatacg agtcccatca
    17221 cgatataaag gatctccagt aatttatcgc ctaataaaat atcaggattt gtaaaaaagt
    17281 ccatcttatt gcccctttcc tggataatat ttcttcgcta atttcttttc tttaagatag
    17341 aagtagacac ttgccacaac taaagccgtg atagccactg gaacttccac taaagcaagc
    17401 tgtcccaatg ttacgtcata tcctagatct ttcagtgttg attggactag caacgcccct
    17461 gctcctccga tgaatagtac ttggccaaag aaccagcaga cattttccat agctgaagcc
    17521 atacctttta gttcttcttc atgcttagga tggacttcta acccttttga ttcgatggtc
    17581 cccaatgcca tcggtaaaat gattggacga acaaatccag caactccacc aaaactcacg
    17641 ttaaaagccg caaagatcat tcgaaagatc ccataaatat ctacaatcat accagcagat
    17701 actttcttga atcgcttgat caaggaagcc gctgattctt tcagaccatt tctttcaagc
    17761 gttcctgttg ccaacataat gatgataaaa atcgccatcc cgcgattgtt tacaaaactt
    17821 gtacctaaaa gttctaacat tccttgaatg ccaagaccgc cagtcaaagc tgtaacaact
    17881 aaagcaacca ttacgatcaa tatcgagtct aatttaaatg caaacccgag aataacaatc
    17941 aagacgccta aaaggctaag ataattttcc atatattttc ctccataaaa tgacaatatt
    18001 tttttaatta taaagcaaaa cacataaaaa atatagttgt tttctcacga aatagacact
    18061 atttttaaat atttttcgtt atttttttat cacaaaaatt atcttactta ttgtaaggta
    18121 aagctttttt taatttattg cccgataata tatagaaata aggtctagtt actgtttttt
    18181 ttaaatgatt aataaataac aacttctttc tatgaaattg caaagcattc atcttctttt
    18241 ccgctaacga tatatccgtc agtgcttggt acactttact aaaaagtatg agattgccga
    18301 tatctggata ggtgtgctat actacagaaa aagatataca atctgccacc gggtaggata
    18361 aagaggatga tcattccatt aggtctttga agggatgatt ttcctctttt tgtgtatttt
    18421 tttatagtta aggaggaatg taagtatggc atggatggaa ttaatcatag caggaatttt
    18481 ggaagtattc tggtcaacga tgatgaaatg gagtgatggt ttttctaaaa tcaattattc
    18541 tttttacaca gtgattggca tgattgctag tttttacttt ttgtcaaagg caatcaaaag
    18601 tttgccgatg agtctctctt atcctatctg gacagggatt ggcgcagttg gttcggtcat
    18661 aattggtgtg gtcttgtttc atgataagct gaatctttcc acttggttct ttgtggggat
    18721 gctactcatc agtatcattg gcatcaagat tactagcggc cactagacac ttggaataag
    18781 tactgatcag aaaaaataag cctttttatt taatattcaa tttttttatg aaaaaataag
    18841 aaataagtcc aatgacctag cgacttctcc actcatcagg tgtatgcttg tactatctat
    18901 agaaaaggag tgaatatttt tgaaaagtct ttcggtccaa gaattatcca agaactttgg
    18961 tcacaaacaa gccctcagcg atgtcagttt tagcttatcc cctggcaaaa tcgttggatt
    19021 agtaggtcct aatggcgctg gaaaaacaac gatcatgaaa gctattttag gactgatcca
    19081 ttactcagat ggaacaatct gtattggtga aaatgagatt tctccctcta gtcataaagg
    19141 gttggaacaa gtaggagcat tgatcgaata cccgggtatc tatccattct taacaggcta
    19201 tgatcatctg aagttattct ctgaaaccaa cgatgtatct gctatcgaca ctatcgtgaa
    19261 tcagctcaag atggaaaagt acattcacaa aaaagcgaaa tcctactcgt tgggaatgaa
    19321 acaaaaattg ggaattgctc ttgccttact gaaccatccg tctatcgtta ttttagatga
    19381 gccgatgaac ggtttggatc cacaagcaac aagagatgtg cgaaaaatca ttaccgactt
    19441 ggctactcag ggcacctctt tcttgatctc cagtcactta ctcagcgagc ttgaaaaaat
    19501 ggtcgatgag ctgatcctga tcaatcaagg aaagatcatc aaacaatgta caatggctga
    19561 attgaaccaa acagcgcaag atttcatcgt tatctctaca acaaatgatc tagctgctcg
    19621 aacgatcttg acagaagctg gatatacttt ggaagatcct gataaaatca agcttttaaa
    19681 agaaaacgaa gaattattag gaaccgtgat ccagctattg tctgcacaaa atatccatgt
    19741 gacagacgtc cagcatctga cgaatgattt ggaatcttct ctattggaaa ttctggattc
    19801 agtaaagggg gaagaagaaa aatgacattg cttgtaaaac aagaattatt taaattgatc
    19861 aagaaaaagt caactgctgt tttatctgtt ctattagttg ttttgctcat aggaacagca
    19921 cttctagcca aaaaatacac gacgatcatt gatccagttg aaatgactgc tcagcttttt
    19981 agtgccacat cttggattgt ctttatcatg attgcagctg caagcacgat catttcgatg
    20041 gaagcacaat atggtacatt gaaaaatctc ctttatcgaa agtattctcg aggagaaata
    20101 ttagtaagca aatggatcac attagttatt tattctgttt atttatacct tttagctatt
    20161 attgtcactg tgttgatgaa actgattctt ttcccttcga tttccttcac ggagaaggtt
    20221 tctactggtc aaacactgat ccaaagcttg ttcacttata ctttgggtag ttatatcggt
    20281 ttatggctga ttttaagttt agtgttgatg attgcttgct ttatcaatag ttccggcgca
    20341 tccatctcag caggaatcgt cttttatttt gcttcgtcaa tcatatctgg tatccttatt
    20401 gcactcatcc aaaaatggga gtggatcaaa tggaatccga tcagtatgtt gaatctgcaa
    20461 aaccaaattg gaaacgaaga gatcatgaag caattgactc atctttctac taatcaaatg
    20521 ctattcggta atttagctta catcgttttg ttcttagcat taggatatct cgtatttaag
    20581 cgaaaaaatg tatgatgact catcaaaaaa gaggctgtga aagagacgtt cagccttatt
    20641 gccgattggc tgtctcttaa acaccgttta ttctgaaaaa ggagctgtga caacatttgt
    20701 gtcacagctc ctttcctagt atgtttttga ataaacgaca ggctcacaac ctctctttat
    20761 acttctttta tacttctata cgtgaagcat cccagtggca aataaaacca ttgcaatcac
    20821 tccgaccatc gtcacaccta taagtaaaat cttatctaag tggatatgtt gtacaggctc
    20881 gttttgctcc ttacgtgctt tgaggtaaaa ataaatgcct ggcaagaaaa gaacaagaac
    20941 aagcattact tcattaaagg ctacgactag catagcccat acttgaaagg cacttgctaa
    21001 aagccctact aaaaattgga cccattcttt ccgctgataa gaaagtttca tttggtacaa
    21061 tcctaagaaa agccacgtga agaaaatagc tgctgtacca agtgtgtaag caaagttata
    21121 agcactgtca gtgaataata atgaaagtaa aaagacattc gtacataacg tcgtcaatac
    21181 gatagcataa gttggtgccc catttttgtt gagttttccc catgctttag gcaaaagtcc
    21241 gtcttcagcc atgtttccca ttgtttcaat cggtaagatc gtccatgaga gccaaacacc
    21301 aactaaagaa atgatcaaac cgccgttgat cacgactgct ccccatttac caaccatcat
    21361 ttcaaacaca tagcccatag caggctgcgc cttgatcgtt tgcagctgat tcatcgtcat
    21421 tgtcccatat ggaagcatgg aaaccaagac gtaaataata atcaagccga atatccctag
    21481 aatcgttgct ttcccaacat ctgattgttt ttttgctcgt gaacctacga cagaggctcc
    21541 ttcaatacca acaaataccc aaagcatcac catgatgcaa ctcaatactt gactggaaaa
    21601 cgggatcggc tgtccattcg cattcaatat aatgtcgcta gaaaagttat caatgaattg
    21661 ttggatcgaa aacgtgctga tagctactac aataaataaa aataatggca ctaatttaca
    21721 gaatgtgacg atagcattga cgaaactagc attttcgact ccccgattca ctagccatgc
    21781 atatccccat aaaaagactg tactcatcac gatcgatgcc agattttgcc caccttcaaa
    21841 tactgggaag aaatatccta atgcgctcat caacaatgtt gcaaaagcta cgttgcctaa
    21901 ccagcatgtg agccagtaac cccaaccgct gataaaacca gcgaaacggc caaaaccttc
    21961 ttctgcgtaa ctaaagatcc cgccttttaa atctggacgt tttttattga tattattgat
    22021 cgacaaggag agcatcaaca ttgctgttcc tacgagtacc caactgaaaa tagctgggcc
    22081 gccagccgat ccggagatgt ccgtcataat accaaatacg ccacctccaa tcgctgaacc
    22141 aattactaag gcagcaagtg gaattaaacc aattcctttt ttctgttgct ctgtatccat
    22201 gtaagcatcc tccaaaataa aaccgctttt atcaaaaagt ataacaaacc ttttttattt
    22261 ttacttaatt aataaaaact ttaatagaaa tataattcta ccggcttttt tctcattata
    22321 caagcaaagc gcctagtccc tttccaaatt acaccaccaa aaaacttaaa aaagatgcta
    22381 tactaaaagg aaagaattgg aggatgatcc atgaacattt tatatatatc gatttcagga
    22441 aatacgcgtg cctttgccaa acatctagca gaatatgctg aaaaaatgca tgcagaagat
    22501 cctgtcaatc ctgaagttac tttaaaagaa atccatgaaa atagtgattt tacgaaagaa
    22561 acagaaccat tttttacttt cgttcctact tatttagacg gcggaaatgg tttagataac
    22621 ggtgatacag aaatcttgac cgaaacgatg cgtgaatatc tggaacatga agacaatcat
    22681 aaactttgtc ttggtgtagt cggtagcgga aataaaaact tcaataatca gtattgtcta
    22741 acagccaaac aatatgcaca aacatttggc tttcctttct tggcagatta tgaattacgg
    22801 ggaacaccat ctgatgtgac acgaatttat cagattttag cagaaaacaa taaataataa
    22861 aaaagaagaa gctgaaaaat agatttcagt ttcttctttc gtttctattg tttctatcat
    22921 attaagcttt gacatccatc acaggtgctt ttgcttttgc tttacctgtt ttttccaaga
    22981 caaagttttt tacacgatcc atattggtta ccaagacgat catggatgtt tctttgcctg
    23041 ctgctttgat cgcttctaag tcagcttctg ctacaagcgt atcaggtgtc acttgctgcc
    23101 cttctgttac tttcacatca aatggcgtac cgcctaagtc aactgtgttg attcccatat
    23161 gaagcaagat ttctaaacca tttggcgttg tgatcccaat agcgtgtttc gttgggaaga
    23221 cagagctgac taccccagta actggagaat agatatgtcc gttttctggt tctacaaagt
    23281 agccttcacc catcatcttt tgtgcaaaaa ctggatcact tgctttatct aaagaaacaa
    23341 ctgttccgtc agcaactgat gagaggatag tcacactttc agctgaagtt tgaggtacta
    23401 ctttttcagg cacttcattt actgtggctg tttcagtcgc taattcttct ttttctaaac
    23461 ggtctttagg aataccgaag aagaatgtag caacgaagcc accagcataa gctgccaata
    23521 atccaaggat gtagcctaac catttaccgt cagagatcaa tgggatcaaa gcggctcctg
    23581 aaggtccaat agcgatagcc ccgacatttc cgatcattcc gatcactgcc ccgccgatac
    23641 ctccaccgat acaagcagta ataaatgggc gtcctagagg taatgttact ccgtaaatca
    23701 atggttcgcc gattcctagg atcccgactg gtaaagctcc tttgatcatt tctgttaatt
    23761 ctttatcttt tttgcagcga atccataatg ccaatgctgc acccacttgt cctgcaccag
    23821 ccatcgcaag aattggaagc aatagcgtac gaccagtttc atcaatcatt tgcacatgga
    23881 ttggagtcaa aatttgatgc agtccgaaca taaccatcgg caagaataat gtgcctagaa
    23941 cgaagccggc aaatgctcca ccaacgccaa gtacccagtt gatcccaccg accatgccat
    24001 cagaaatgaa acctgctaaa ggcatgatga agaaaatttc aatcagaccg atggcaatca
    24061 atgaaagagt tggtgtaacg atgatatcta ccgcatcagg tactacttta tgcagttttt
    24121 tctcaacgat tgacaatagc caaacagcga agatgacacc taggatccca ccttgtccag
    24181 ctgcaagagc ttcaccagta aataagtttt tcagtggtgc ttccggattc atccctgtca
    24241 gcatcacaac ggccccgata actccaccaa gagttggtgt tgcaccaaaa acttgtgcag
    24301 cattgatacc tgtatagata actaggtagc taaacatccc gtttttcaag atgttcatca
    24361 catcgatata ttgttgccat gttgctgcat caagtgttcc ggctgtgact aagtttgcta
    24421 aaactgcggc aatccctgcc acgatcccag taccgacaaa tgcaggaatc attggcacaa
    24481 agatattaga aatgtctttt aataccgatt tgattttaga tggtttttgt ttcgattttt
    24541 gtgccgcttt catttcttgt gcccgttcgt ttacacggtc ttttccagat gctgatgaat
    24601 ttgctgtagc tgtacttaca gaagaaagtt tttctcctaa tttcacgcct gccaaatcaa
    24661 ccatttgctg tgctactttg ttcactttcc caggtccgat gatgacttga agctgttcat
    24721 cttctactac accaagcact cctggaatcg cttttaattc agagacattt acaagatcat
    24781 cattgatgat cttcatacgc acacgagtca tgcagtggac aatgctttct acattgccca
    24841 tgccgcctgc ccctttataa atttgttttg ccagttcttc caaagtcagt tcgttttttg
    24901 ccatggtctt ttccccctca taaggtccct tttacaaatc cgtttgcttc agctaatttt
    24961 gcttgagctt cctctttatc actatttgtc aaaatcatga cgatagccag cttaacattt
    25021 tgatctgctt cttcaaagta cttttcagct gtttcgtaat cagcttctgt cgcttgcatg
    25081 atgattcgtt ttgagcgttc gattagtttt tcatttgttg gtttgacatc gaccatcaag
    25141 ttattgaata ctttacctat accaatcata ctgatagtag aaatcatatt caagataagt
    25201 ttttgtgctg ttcctgactt caatcgagta gagcctgtca aaaattctgg acctgcatcg
    25261 acttcgattg ggaaatccgc gtgtttactg atttcggctt gtttattgca agaaatcgtt
    25321 cctgtgacag caccgatcga ttttgcgtaa tctaatccac cgatcacata tggtgtacga
    25381 ccgctagctg cgataccaat aactatatca ttttttgtca aattcagctt gattagatct
    25441 tcttgcccta gctcataaga atcttcggcc ccttcgacag ctacagtcat tgcttcttct
    25501 ccaccagcaa ttagacctat gaccatttcc ggttctacac cgaatgtcgg tacgcattca
    25561 gcagcatcta acacacctag tcttccactt gttccggctc ccatataaat cagacgtcca
    25621 ccttttttaa aggactcaat cgtgttagca atcactggtt cgatacttgt cagttctttt
    25681 tcaaccgcag cggccacttt ctgatcttct ttgttcatta gttgaacggc ttgagaaata
    25741 ctcatctcat ctaatccaaa agtttcttgg tttctgcgtt ccgttgttag gttctctaaa
    25801 ttgattttgc tcatttttat cttccttctt ttcttataaa ttgatagttc ataccagctt
    25861 tgatttggtt gatcaatggt agctcttctt tgataacttt agctactaca ttcacttttt
    25921 catccgctgg caaatcatat ttggttaatt ggatctcacc catatagcgc aaatactttt
    25981 cgttatctaa agtaatagac cctttcatac gcgtagctgt atttcttgct gggataggcg
    26041 gaatctctcg aaaacgtgca tctacgcttc gtatgacatc tcttgcatcg tcttgccgat
    26101 tcgtatgctt ccctaatata tattcgtaaa attcttcatc tacaggttcc gtatggagca
    26161 agactgtctg ttctttttgg aaagaagcaa attgctcctg aacgttttct gacagacctg
    26221 catctccgat gtaaacaata tccgtgttac ttgctaatag atctagagcg cctgcaagag
    26281 gatggatccc tcgatgttct tccaaagttg gcaatccttg atatagtggg cctctcaact
    26341 ctgtatctcc tggtacaaaa ccttggacaa ggagaccttg aattttgagc cattgatttt
    26401 ttttctgata ccattctttg tccagccctg tttcgggacg tggataataa ttatgccaag
    26461 cttcaatctg tgagaaatca gcttctgctt ctttcaattc atcgatatct ttaggcgtga
    26521 tcgtgctagc attcaaactg attttcaatt gatgtgacca gtcggcaatc tgctgattgg
    26581 aaatatgata atccattctt aaacctgtta caccaattgc tttcaaaggc gttagatctt
    26641 ctaatgaaaa acccgctcgt tccaatgcct ctcctgaaat atcgaccatc aattctagtt
    26701 tattcttttt cgcttcgctt cctagttcga tcaatcgctt ttgatatgcc gaaacatcat
    26761 cttctggtat atgcatggaa gtgaaaatac cgacaaagcc attggatgcc atttggcgga
    26821 tatactgttt cttctcttct gacaaagaat cgttcataaa tattgaaaag ccaaacatat
    26881 tatccctctt tttctgtatc cgtcttcatt tctatgacta gagtatatat ctaaagaaac
    26941 aaaatttcaa tacttttatt aaaataatta aatttcattt ctaaaggttg agtttatatt
    27001 atcaaaagaa ttgtcataac ctaaaaaagg cactgctaag caaaagcagt gccttgaaat
    27061 attttattta aataacacaa aaacataatt actcataaga aaccaattct gtattatcta
    27121 ccatgaatgt aaagaacccc tcattaggca tcggatgaaa ataagcattg atcactctta
    27181 tcaaggcatt atggcaaact aacagataga cattttcttc gtcttcgatg cattctttca
    27241 ataaaggaac gatccgtgca tacacatcta agactgactc cccatttggg aatcgtacag
    27301 cgaacgctaa acgtgctttt tgaaagttct catctttgct tggcagtcca tcataatccc
    27361 caaaatccat ttctaccaat cgttcatcaa cggtcagcgg caagctgaga cgatcagcaa
    27421 ctgcttgtgc tgtatctctt gctcgttgaa gaggggaatg gatgattttc gtgattggaa
    27481 caggcaaatc tactaccttt tctgctagtt tctgtgcctg catctgtcct ttttcagtca
    27541 gcggcacatc tgcccgtccg caaactaatc cttgtgcgtt ccaagttgtt tctccgtgtc
    27601 tcgttacata aagcatgtga ttctctcctt cgttggcaaa gttcccctta accatacctt
    27661 gttgttcttg agattacaag ggttagattc tctcaagaga aataatctca gtagatactt
    27721 tatccaggaa ggtttgatca tgttccacta gtaacatggc aggtttcacg ttcaagatca
    27781 gttgttctaa ttgttcttga ttgaagacat ccaaataatt caatggttca tcccatgtat
    27841 atagttcagc tggctgtgat aaagatttag ccaattccac ttttttccgt tggcccatac
    27901 tcatctgctc gatcttgtta tgaaaaacat ctctttccat cccaagcttt cggaggttgt
    27961 tcaaaaatgc ttggtagtct acttggtttt tctctgcaaa ttccgccaac gttcctcgat
    28021 tgtcttcata attttggctt gcataactaa tgctcagatg ttttggctgg tatttttctc
    28081 ctataacctt gccgttgaat gcccccagaa gataatggat aatggatgac tttcctgcac
    28141 cgttaggacc tgaaatcgcc acccgctgat gaggctcgat tgtaaaatga attggctcga
    28201 ataacagatt ttcataccct aattgaagat cttctacgct taaaagtcgc ttatggtgag
    28261 acgcttggct attcatcgtc aaagaatcga tatattcgat atcttttaat agtttttctt
    28321 tctcgctgat ctgggtctcc atccgattca cgattgcttt ggatcgtttc atcgtccgtg
    28381 cagcatcagc acccactgct cctttattca ctcgtctaga ttcagtatcg atgaatccga
    28441 cttgtttctt tgttttatct ccttctcggg aacgagacca ttcggctttt tcagctgctg
    28501 ttttctttag cctactgact tcttttttca atttttcatt ttgagccatt tcaaattcat
    28561 cacgaagttt tttctgttct tcatagatag agaaattccc ttgataaagt tccagttgac
    28621 ttttttcgat tgctaaaaca tggtccacta cttcatcgat aaatccccgg tcatggctga
    28681 ccacgatgaa gccttgtttt ttctttttca aataagccgc tacttgtttt ctaccagaga
    28741 tatccaaatg attcgttggt tcatcgatta acgggaaatg agtgtcatcc acaaataaaa
    28801 gtgccagtag gacttttgtc ttctctcccc ccgatagtgt gctgaattct ctccataaga
    28861 tttcaggatc tgtttgcatc aattggagct ctctttcgat ttcccatatc tcaaaatccg
    28921 taatatcatt taacacgtaa taggttaaac gttctttatc ttttgtctgt tgcgggaaat
    28981 aggcaaattc ttgctgatgg attacttgcc cttgataagg tagtttgttt tgtagaatat
    29041 tcagtaaagt tgtctttcct cgaccgtttc gtccgataag tcctagtttc cattgtgtgt
    29101 caaaatttag atttgcttgt tcaaataata atgtgccttg gctgtcgtag ccgaatgtca
    29161 gattttttat ttcgatttta gacattttcc atcacctcgt aaaaaaagac cagtcctatt
    29221 ttcagaactg gctaaagagg atgcaagggt tggatacaca caaaaagacc catacatcga
    29281 aaaaaccaag ccaatcccga aaatctgcac actgcttcct tgtctccaca aggtacagtc
    29341 tgaatgcagc tttcatcaat cgacttaatt tttcaatgca cggatccctc actttgtcct
    29401 atatttgaaa ttacgatagc ataaagaaaa tgattttgtc aactacttcg ggaggatggt
    29461 gaaaaatctg ctaagtgtct gtcctaagat ttttgttttt tctaatgttt gcctgtgttc
    29521 cattgcttct tctaaaccta ttggacccaa atgaatactt aagacaattg ggagaaaatc
    29581 agaaagtcct tctagctctt ggtctctact tccgcaaata gcgattattg gttttcgata
    29641 tttttttgca agcactgcta caccgtatgg aacttttccg tggatcgttt gctgatcgat
    29701 ccgcccttct cctgtaatga tgacatctgc ttgttcgatc tttttttcca atccagtgat
    29761 ttggctgact aaatcgaaac cagaaaccat cttcgctccc aacaaaaaaa gggctccacc
    29821 aataccgcct gctgctccac ttcctggaat atcatctaaa tccacctccc agaaatcttt
    29881 tatttgctga gcaatctttc ctgcttgttt atctaagaaa gctagcgtcg tatctattcc
    29941 tcctttttgt cttccaaatt gccaagcagc tccttgttga cctgtatatg gattggtaac
    30001 atctgcagcg ataatcaatt ttactttaga aacaagaacg tttcttcgat caaaatgaag
    30061 ttttttagta gataataaag gatttccttc tgtgtaccct tctaaagaag cacccaagga
    30121 ttggagtaat cctagaccac catcgctaca cccgcttcct cctaaagtga caataatttg
    30181 ttctacttct tgaagcagtg catctttaat aagttctcct aaggcataac tggaagcaag
    30241 tcggatagtt tgatccgttg gtttatcaag tagatctaac cccaatgctt gtgcagattc
    30301 gatgactgcg gtgttttttc cttgccaagt tgtcaataaa tagctgattt ttcgctgacg
    30361 gaataagaga tccatcccat caatggtacg ccatgttccc ctgagcgcgt aatagattgc
    30421 ttctgcagta ttttctccgc catctgcaat cggcacattg atttttcttc ctttacgatc
    30481 ttcccagcca tttagaactg cctgatttag ttcaaatgaa gaagcacttc ctttaaatga
    30541 atcaattgcc gccaaaatat ccatctgtat ccctcacttt cttcttaagt aagacacttt
    30601 ttttcatttt tttcaaacaa aacaatgcta ttttcttcca tttttctata tactaaaagg
    30661 gaactcattg aataaaggag agaaaaaaca tgaaaatcgc aatagctggg gccggagcaa
    30721 tgggcagccg aatcggattg atgctacatc aaagcggaaa tgaggtcctc ttgatcgatc
    30781 gttggcctgc tcacattgaa gccattcgta caaatgggtt gattgctgac tttaacggta
    30841 aagaagtggt cgcaaaactg ccaatctatt caccagaaga aatcatcgaa agcaacgaac
    30901 acgtggactt gatcgtagca ttaacaaaag ccaatcaatt agatgatatg ttttgttcga
    30961 tccaatccat tattactgac aatacttatg ttttgtgttt actaaatgga ttaggtcatg
    31021 aagatgtact ggaaaaatat gtgccaaaga aaaatatctt atttgggatc acgatgtgga
    31081 cagctggtct agctggtccc ggaaaagtca cattgttagg tgatggagaa atcgaactag
    31141 agaacttgga accagaaggc gaagcattta caaaaaaagt tgtagaagtc tttcaagaag
    31201 caaatttaaa tccgatctat agccataacg ttcgttattc catttggcga aaagcttgtg
    31261 taaatggaac attgaatggt ttatgcacga ttttggattg taatattgct gaactaggcg
    31321 cgcaaaaagc ggcagagagc atggttcgaa caatcgtatc tgaatttgct tctatcgcag
    31381 caaaagaagg aatcatacta gatcaagaag aagtgtacca acatattgct tctacttatg
    31441 atcctgacaa catcgggttg cattacccat ctatgtacca agacctgatc aaaaaccatc
    31501 gtttgactga aatcgactac atcaatggtg ccatttggcg aaaaggacaa aaatatgata
    31561 ttgctacacc atattgtgcc tttttgaccc aattagtcca tgcaaaagaa ggaatccttg
    31621 gcgcagaata aaaggctctc tgatgagaat cagctgaatc agcagctggt tctctttttt
    31681 taagcccaat aaaagatcaa aaagaaaaca caactctcat gatgccataa gaattgtgct
    31741 tgtttgtttc agtacataat ctcttcaaaa tgtaggtttt ttatttgttt tcaacagagg
    31801 agaggtcata ttcgtattgc tcataaatag tggaatcgtt agtgataaat cgttttgccc
    31861 aattttctgc atcataaaat tccaatagct ctgataggat tggcttaaat ggtgtatgca
    31921 gaaattccgg taattcatct aaatcacgtt ctcgaagcaa aaaaatattt tcaggacgat
    31981 aaaaatgata atgagcaatc gcttgattat ccgtcactag tttcttttga aaatacatgg
    32041 attcaaatgt ccgcaaagtc actccttgct gaccctcccg caacagttcc agaatcccgc
    32101 gcgattcccc tgtttttttc aggtactctt cataagtaag atatccaggt tgttcattcc
    32161 ctcgatgtgg aagaatgaaa tagttggtcg taacatctct ttttataaat tcctgcttat
    32221 agctttctgc tcgttctttt ctcccattat tcgttgcacc aaaaaataga tccgtctttg
    32281 gttcgctaga agaaaaattc atatgtttcg aataaaatga actattatgt ttcagcccaa
    32341 gttcttttgc ttccaaaaaa tcaaaatgat aaatctcatc gattgccggt tcatcttgtg
    32401 cctgcttcag catcgcctcg tgttctgctt tgaagtgatt ccaaaaaaat aacacaattt
    32461 ttgcattggt aaatgggcgc acgcgtcgag ccatctccat atcaaatcca taatctaaaa
    32521 aaacgatcag ctgataattt gccaactgtt gtctagctga tttaatgaat tccgactgta
    32581 gtaaatcttt tccggaccaa acatctaaag agcggatgga aggcaaaaaa taaagtgatt
    32641 tattttttgc aactagcagt acgtcatttt tgttcatctt ttccgcctca ctttctctgt
    32701 cactttttcc tatgatagcc tgtttaaata gtaaaaagcc agtaaaacgt ttcctatatt
    32761 tgtatgaaaa tttaacattt gtatgaaaat atgatgaaat aaatcattct tctattaaaa
    32821 tt
//
LOCUS       DAFGZE010000030        30731 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.030, whole genome shotgun sequence.
ACCESSION   DAFGZE010000030 DAFGZE010000000
VERSION     DAFGZE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.030"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(137..766)
                     /locus_tag="LTU54_001461"
     CDS             complement(137..766)
                     /locus_tag="LTU54_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296183.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKKDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNERKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            complement(763..2139)
                     /locus_tag="LTU54_001462"
     CDS             complement(763..2139)
                     /locus_tag="LTU54_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL8296184.1"
                     /translation="MAMKLLEKVKNRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISDTPHMLVAGTTGSGKSVLLNEILVTAICHAMPDELKLG
                     IVDPKRVEFGRYKKLPYMLADPITDMDEAYEFFEYLVILMNERYKLMEKAGVQNITQY
                     NKYAEKHGIEKFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMFIKLNGGNKIRAQG
                     AYISNEEIEAIFDHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     VDPEPQTSNNTNNEQPISSKTKPKTTDPKVSVKVTITTDNSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            complement(2219..3481)
                     /locus_tag="LTU54_001463"
     CDS             complement(2219..3481)
                     /locus_tag="LTU54_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296185.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVANFSKSVYLVGRALVVSAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATRGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETEKSTASIAVSNSQFAFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            complement(3515..3841)
                     /locus_tag="LTU54_001464"
     CDS             complement(3515..3841)
                     /locus_tag="LTU54_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296186.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDD"
     gene            complement(3861..4730)
                     /locus_tag="LTU54_001465"
     CDS             complement(3861..4730)
                     /locus_tag="LTU54_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL8296187.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGNVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            complement(4961..5767)
                     /locus_tag="LTU54_001466"
     CDS             complement(4961..5767)
                     /locus_tag="LTU54_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296188.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTAENWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            complement(5855..6655)
                     /locus_tag="LTU54_001467"
     CDS             complement(5855..6655)
                     /locus_tag="LTU54_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296189.1"
                     /translation="MVGISKTALDSLAKLFSSRFDSAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            complement(6994..8073)
                     /locus_tag="LTU54_001468"
     CDS             complement(6994..8073)
                     /locus_tag="LTU54_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296190.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDGGSFDIGEAEGKVNPYTGDVFSKNQT
                     KDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLPLSGKGHQTVI
                     AGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQNSTLTDIPDL
                     DMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDSEYVSDKTETV
                     TSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTGITNKHQKMLL
                     VIWTVIGALASLNVLILLIKEFRKA"
     gene            complement(8063..8926)
                     /locus_tag="LTU54_001469"
     CDS             complement(8063..8926)
                     /locus_tag="LTU54_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296191.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKIILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            complement(8995..10830)
                     /locus_tag="LTU54_001470"
     CDS             complement(8995..10830)
                     /locus_tag="LTU54_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL8296192.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANKTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            complement(10879..13269)
                     /locus_tag="LTU54_001471"
     CDS             complement(10879..13269)
                     /locus_tag="LTU54_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBL8296193.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDSNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDEQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            complement(13539..14786)
                     /locus_tag="LTU54_001472"
     CDS             complement(13539..14786)
                     /locus_tag="LTU54_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL8296194.1"
                     /translation="MNMKKNSLAMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNINK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            15179..15595
                     /locus_tag="LTU54_001473"
     CDS             15179..15595
                     /locus_tag="LTU54_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296195.1"
                     /translation="MNKKRKLSVLITLVSMLAILSGILSIKIFNQINTSANTKNEIVI
                     ENTSNSKEISTTKNKDKSDETTTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKDEELYSFVK"
     gene            complement(15612..16349)
                     /locus_tag="LTU54_001474"
     CDS             complement(15612..16349)
                     /locus_tag="LTU54_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296196.1"
                     /translation="MADRKLEKLLEETWDPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENRGTWDFWANLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISEQDGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVNITNRLDYDSWADIALEKYIYSTLKEFIENRMNIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(16767..17330)
                     /locus_tag="LTU54_001475"
     CDS             complement(16767..17330)
                     /locus_tag="LTU54_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296197.1"
                     /translation="MQNHEITTAKKAIGILSIILILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            complement(17317..17844)
                     /locus_tag="LTU54_001476"
     CDS             complement(17317..17844)
                     /locus_tag="LTU54_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296198.1"
                     /translation="MNSKTKDAIIISLLSGMLLAMLVGILYFNKQNQDIEAEKHSVQI
                     ENELTFFKEVKPNEVDGLQRDLENDIQEMFKGSDRSKFANQSGEEFLRKLFIVTGKPP
                     VTNESSEQEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            complement(17869..19200)
                     /locus_tag="LTU54_001477"
     CDS             complement(17869..19200)
                     /locus_tag="LTU54_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HBL8296199.1"
                     /translation="MVQFTGIAMSVITINPQVLHADGIATYDAQFNCTTEGSGSGTDS
                     DGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRTVEGYY
                     GDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMFKG
                     DGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMKSK
                     GMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEGKI
                     NGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFQGELQGQCTELTWAYMSQL
                     YDGNQPTLGNGGYVWESYKSAGAKVTNKPIVGYAFSALDGWLWAVGSAGHTGVVIAVF
                     DDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            19326..19802
                     /locus_tag="LTU54_001478"
     CDS             19326..19802
                     /locus_tag="LTU54_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296200.1"
                     /translation="MTDKTSDLPKLDPKISQVKSKKDGKTKNAVKETSSLLLDIPMSS
                     FDEILDANTDETSVKIASHVGMNSQNDYFYNLLNSLRKLEDKKNQVIASKAPDLTTDL
                     DAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            complement(19917..20149)
                     /locus_tag="LTU54_001479"
                     /pseudo
     CDS             complement(19917..20149)
                     /locus_tag="LTU54_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(20152..20469)
                     /locus_tag="LTU54_001480"
     CDS             complement(20152..20469)
                     /locus_tag="LTU54_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296201.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            complement(20484..21315)
                     /locus_tag="LTU54_001481"
                     /pseudo
     CDS             complement(20484..21315)
                     /locus_tag="LTU54_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21368..21697)
                     /locus_tag="LTU54_001482"
     CDS             complement(21368..21697)
                     /locus_tag="LTU54_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296202.1"
                     /translation="MKLEGSLQNRMMEGSTTNKEIVVGMGATELLYTDRNPYTVIEVK
                     SKNRILVQADGAINKATFPDQEWEYSRNPEGQILELVKTKNGWKVLKEDIHFYIGDRE
                     KYYDPSF"
     gene            complement(21825..22070)
                     /locus_tag="LTU54_001483"
     CDS             complement(21825..22070)
                     /locus_tag="LTU54_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296203.1"
                     /translation="MKEIEGIKKRNQERLELLNENRNRNRMFSYYESMRCLNKSRSEI
                     IESSKYKQLMEERVSLIQEIETESIFPDHSPKVRFYN"
     gene            complement(22161..22448)
                     /locus_tag="LTU54_001484"
     CDS             complement(22161..22448)
                     /locus_tag="LTU54_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296204.1"
                     /translation="MVEVEFGSKEWFEELNSMIKFPEKFTFYYNKRDNIIQVALLETK
                     GRLENTRWSMIKPSALRFHPKDLTAVVAVKEVQDSQEYYEVVKNLKDIWCK"
     gene            complement(22638..22820)
                     /locus_tag="LTU54_001485"
     CDS             complement(22638..22820)
                     /locus_tag="LTU54_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296205.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENVEDLRLIGE"
     gene            complement(22857..23657)
                     /locus_tag="LTU54_001486"
     CDS             complement(22857..23657)
                     /locus_tag="LTU54_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296206.1"
                     /translation="MSKDLRELKLRKKQYNQYFSSLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSRSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            complement(24275..24535)
                     /locus_tag="LTU54_001487"
     CDS             complement(24275..24535)
                     /locus_tag="LTU54_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296207.1"
                     /translation="MAYENLQLVVAEVSGDIYLTKVKDGIMDTNYRRVATDDVLSSST
                     EWFRKNEESAVHFEGIDGNIHSLFYTKDKEKAKKILDILKGR"
     gene            complement(24726..25094)
                     /locus_tag="LTU54_001488"
     CDS             complement(24726..25094)
                     /locus_tag="LTU54_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296208.1"
                     /translation="MWNNKLKTGLLLIVISCAMMVGMIIQREQSYFEVSANNVIERCH
                     YGQHFWTGEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSNLMMKKV
                     RKLAQNYLEEKEGNSPIDKN"
     gene            complement(25256..25786)
                     /locus_tag="LTU54_001489"
     CDS             complement(25256..25786)
                     /locus_tag="LTU54_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296209.1"
                     /translation="MSQNIGAVKQLIEKDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAEEKSSILEISQ
                     NDKAIQVKCVDPTGKIIVGFDIERKSKIDFITQLTKINNLNILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFSNA"
     gene            complement(25802..26167)
                     /locus_tag="LTU54_001490"
     CDS             complement(25802..26167)
                     /locus_tag="LTU54_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296210.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDQQFNQTHHNIILLVDQAKRRLEKAEYELLDQGIICLDKGIRRYYSAELKKQ
                     NVDRHALKHEILELIMQRI"
     gene            complement(26173..26916)
                     /locus_tag="LTU54_001491"
     CDS             complement(26173..26916)
                     /locus_tag="LTU54_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296211.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKAKSIRELKTEVEKIRLGLTREEISRLSENSSFVIQKEKEKHLLHRELIIEKYYDV
                     DLPDQLRIIDEFSEKKWKSEIYPSLIQYKASGKPMYNYTGFLNYTQYKYAFRKLKGIK
                     QADIALYEMKKMDRLKLIQELVQNTWNQAIVELEKENGKILTNEYLIVNEIEELIGLF
                     SCENGEVFLKSSPIYSILDSPYQFIQVRR"
     gene            complement(27047..27769)
                     /locus_tag="LTU54_001492"
     CDS             complement(27047..27769)
                     /locus_tag="LTU54_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296212.1"
                     /translation="MKSTKWKIVLLSTAALITLTACGTTNSADNNKDTKSSSTVVTKN
                     SSTSASTTISSMTENNAEKYQKYEDINKGQKPEEVYTDFINTVKNDEDYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYHAVAVDITDGV
                     PEWIQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            complement(27775..28020)
                     /locus_tag="LTU54_001493"
     CDS             complement(27775..28020)
                     /locus_tag="LTU54_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296213.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLVLNKRKKDR"
     gene            complement(28031..28615)
                     /locus_tag="LTU54_001494"
     CDS             complement(28031..28615)
                     /locus_tag="LTU54_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296214.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSIT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTIISNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIDTSIKELREYVQNPYNFQYLAGRINLV"
     gene            28737..29651
                     /locus_tag="LTU54_001495"
                     /pseudo
     CDS             28737..29651
                     /locus_tag="LTU54_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(29856..>30731)
                     /locus_tag="LTU54_001496"
     CDS             complement(29856..>30731)
                     /locus_tag="LTU54_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HBL8296215.1"
                     /translation="STHSDTFGHQEQTNYNTHYQTYGYHPLVAFDGLTGDFLKAELRS
                     GNQYTSKGVKEFLTPLLEHYNHSLPNTDILVRGDSGFATPGVYDSCESKKSHYVIRLK
                     NNRRLGQIAEKSVLYGDNQKWEEREVQYFSTTYQAQSWSQSRRVCIRSTREAGELLFR
                     HEFIVTNLSENVSPDTIFSIYAKRGTMENFIKEAKAGFYFDKTDSPRFLENHVRMMLS
                     LIAYNLVNFLRTIGFEEVQKGMTIHSIRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNE
                     FYRVFARLRRASQWI"
BASE COUNT         8867 a         6023 c         4263 g        11578 t
ORIGIN      
        1 aaataatgtt tgttaatttt aagaatgcga ataataatat acttatcacc tctatattca
       61 atggattcac caatacttac tttttcgtta aatccctctc tatatagttt tagaatagtt
      121 aacacaattt tcacctctac tgccaaacaa atttagcttt atcatgccat gtttcagaca
      181 ttactttttt accattttcg tcaaatatca ccgcttccca ctttgctgtt ccgtccggct
      241 gtttacaaat atctgcaaac cataggattg tttttcgttc gttgaatttt cttactttct
      301 tctcaaattt attgatctga actctatttt gattgctgtt ccaacctact aataggcgat
      361 tatctgattc tgtaccatga aaaccgatgg gaacgatgtg ggttctatca aatgcttctt
      421 ttccttctgg atagagcatt ggtctaagtt tcctacgttt caagtcatct cttgcttgat
      481 accgccatgc cgattttgga ttgaatttaa tagctatttg atgcttgctt ttgtcatgat
      541 aaaaatatgc cacatctgct tcagcgccaa gcaacttttc tcttgcacta tttgattcaa
      601 acggtttatt cacttctaaa accttcacac ccaaaattcc atgatcaagc atctgtttag
      661 gagttaacac cgctttttta tcttttttct tagctcgttt cacaactact tcatctgtat
      721 atttatatgg cgtaagccac ctcaaaaaag aactgacaat gatcatttat gcacctgttt
      781 tttggctttt aacatttttt gcatgatagg tgaagtagtt ccgtttactt gtttcttctc
      841 agaattatcg gttgtgatgg tcacttttac tgaaactttc ggatcagtag ttttcggttt
      901 agttttagat gaaatcggct gttcattatt cgtgttgttt gaggtttgcg gttcaggatc
      961 aactttatct aatgctttta aaactttttc taatttttcg gcttgaagtt tttctagtgt
     1021 tttagctcct tgtttttctt tttcttcttt gtttttttca agttttagag caaacgtact
     1081 cttcttttca attgatggct ctttagatga tgaatttgct aaaggtgtgt tcaaatttct
     1141 tttagcagat tttcttacag gagctttacc aattccacca aaatcctcgt cttcaccgcc
     1201 ctcaccacga gattcagcta cgattgctcg gtaatcaaca tattctggtt ctggcatagt
     1261 atttctaagg tgatcaaaga tcgcttcaat ttcctcgttg ctgatatagg ctccttgcgc
     1321 tctgattttg tttcctccat tcaacttgat aaacatatct ccctttggac ttaggctttc
     1381 tccaccaccc tcatctaata cgatcctact ttctaaatca cttgctacca tcaagcagac
     1441 tctagatggg aaatttgctt taataatacc tgtcacgact gtagatcgag gtgattgtgt
     1501 tgcaagaata acgtgaattc ctgccgccct tgccatttgt cctaattgaa cgatcagccc
     1561 ttcaacttct ttgtgtgttc caactaattg cgaatattca tcaaccaata agatcacata
     1621 gggaaatttc tctattccat gtttttcagc gtatttgtta tattgtgtga tattttgaac
     1681 gcctgctttt tccatcaatt tgtagcgttc attcattaag atcacaaggt attcaaaaaa
     1741 ttcataagct tcatccatat cagtaattgg atcagcaagc atatagggta attttttata
     1801 acgcccaaac tctactcgtt ttgggtctac tatccctagc tttaattcat caggcattgc
     1861 atgacagata gctgtaacaa gaatttcatt cagtaatacc gatttccctg atcctgttgt
     1921 tccggcaact aacatgtgtg gcgtatcgct gatgcaaaaa tgagcaattg tgccatcagg
     1981 cttaattcca aaaatagcat ctgtcgggtc tttagccttt gtgttcaaca tattgacact
     2041 gaattttcca ttaagcttta tattaggttc tttatctagc aaaatagatt cagctttttc
     2101 ggctctattt ttgactttct ctaacaattt catagccatt tctattcctc tcttttctcg
     2161 gtacttttct gtttgtgaac tggtagtttg tattttctgt ttctatttgt gatgtcttct
     2221 aaaacacgct attgatcatt gatggtacat caattggaat acgatcattt tctcctaatg
     2281 gtaaagtaat ttgaatacgc cctgcgttga tccgcacgat tacacctgat atatgcaagt
     2341 aagtttcaat ttcttctttc attgagctta gttgaatttt tctggtacga gataaagtaa
     2401 acaaatattc aatagatgta ttcccaacgt gcatatctgt tagacttgca ggtacgtcag
     2461 cagaagatag ataaagttct aaagattgct tttgagtttc ggcatatttt ctagcttttt
     2521 cggtttggtt ttggatattt ttggagttat ctgcaaagag attgattgga aaagcaaatt
     2581 gtgaatttga aactgcaatg gatgctgttg atttttccgt ttccttgttt ttggtactta
     2641 ctctttcctt acgttctctt tcaatgttta ctgatgctct gaagatgaag taatcacctg
     2701 aaacttcgtc ttctttttcc ccaaatttag ctcgccctct tgttgctctt gtcaggatcg
     2761 accctaaatc ttgaatttgt cgtaaaactg tttctctaac tttcttccct tttggttgcg
     2821 gtatttttac gatatattct cgaatatctt cattgacact tagatcacgt cttgtatgaa
     2881 cagaaatttt gatattttta agcgttgata gcacatcgga ttctaattgc gttttgtttc
     2941 gttttctttt tttgttgttc gtttcatctc ctataatctt gtttggatca tacttcacat
     3001 ccatagcttc tagagcttct cttttcaacc ttctactttc catatcatca aagaatggtc
     3061 tttgttctcc atagtgatgc ctgaacatca aagtaaccaa atatccgaca aagaataccg
     3121 ttcctgcttc taatactaaa atagagatat ctaccaagtg tagctttgca aaaaagcttt
     3181 gtacattata tgaataactt aaataatttg gaaaaaacgc attgagtaac tctttaaaca
     3241 tctcttttaa tcctaaatca tctgttccgt taacatcatt taacgagacg agaatactta
     3301 ctaaggtatc tacaagcagt tgtacaacct ttgttaccca atgcaaaata tatatgccaa
     3361 ttgcactaac taccaatgca cgtccaacca gatatactga tttagaaaaa ttggctacag
     3421 gataatattt ttctccatcg cttcgcttat attcctttaa ttcaatggat ttttcgctca
     3481 tctgaatatc tccttatttt ctttctgatt ttggttaatc gtcacttatg ctgtcctcac
     3541 acatctgcgt atctttagga atgattactc tgatttcgtc taacatttct ctctcaatga
     3601 tcatcgcccc ttttgtacta acagttttct ctgataattc aacacatttc cctatttcag
     3661 aatctggatc aaaaatagcc tgttctacgt caaaatgatt cttagctaca tacacgatac
     3721 tcattgtttc taactctcta gcaactgaat atttaccctt cttcagaaga tagtagccat
     3781 ttcctttttt tattcgattt gcaactgatt tatctaatcc attgatgctt attgtttcca
     3841 attcttcacc ttctcaattt ctaatctctt acccaagtac cattttgata gttgatgttg
     3901 attccttttt gattgttaaa tacataaaca ttaaggttta ccgtttttcc attatcgtta
     3961 actgaaaaag cttcgacatg agaaccacga ggaaccaatt cattcccttt ataaattgga
     4021 tcaactttat acaatacttt ttcattctta tttttataga ttgcatcacg tacaatcgtt
     4081 tctgagtaag ccatccctcc attctgatca tttttcccaa cattttgcgc tctcgttcca
     4141 agtgttaaat tgtgtacctc catatctcct cctagcgacc atgctaatga atgacttttg
     4201 ttatagagat aacctttata gccttctagc tgagtgattt cattgtttga atctttgccg
     4261 acccaagtat ctctctcttg atcaaatact ccatccttgt attctcctgc aatatggaca
     4321 gaacttttga aagatggacg ttccacattt ttagatgagt gagaatttac agcatcatag
     4381 gtgatcaacg cttcaacatt tcctgctctt ccaagttgat ctaaatctgc ataatttacc
     4441 caaaaggttt gactgccttt ttctgataat gcttcatggt cttcttttgt cagtgtatat
     4501 ttcccatcat taatgtttac gatattattt ggatatttgt ttggatcata acgtatccct
     4561 aaaaattctt ttgattcact gtctgcttta ttacctacct gaacactact tttgatctga
     4621 aaatcatgtt cctgagtgct gttattctga aagtaaccct tgccactaag accaaccgca
     4681 actaaaacca aaattcctaa caacgatcga ctaaacttct tctttttcat accatcattc
     4741 cttttattgt gttatcataa taagataaat atataataaa ctatattgta aattttgtca
     4801 aatgtatttt tatttgttcc cactataaac cttgatatat agccaattta aaaaaggatg
     4861 acaaaaaatt atcaagttaa aaaaataaat tttgaaccaa attattttta ataaaatagc
     4921 tagttttttt atcaaaaaaa tagagctttt cagctctatt ttattcaata tcattttgat
     4981 caattaataa tcgtacactc caattaaccg agttttgatc actcaaatat ttataatctt
     5041 tgtcatcaaa aaccagttgc caaactctgg tacgaacagt ttcttcgtcc acttcctcat
     5101 ctgaagagta ttgcgataat gcttcttcaa ctatatactt tacaacttcc tcaaaatcag
     5161 taggaacttt atcaataacc acttcatagt cttcttgttt tacgatattg ggatcaatat
     5221 gttcatccaa acatacctca gtgagttttt ctttcacctc tgtatctctt tctgttttta
     5281 ggtactctgc atagtcttcc ttagaccatt gtttttcaag atcatccgct atacttttaa
     5341 taaaggattc tttatttaac caattttctg cggtatctat accttcaaac tcaaaagctg
     5401 ttccttctat acgtgatttg acgaaagcat caattttctt gtattgatta ttcatttccc
     5461 atgaaacaca ctcatcttct tgcgtaatat cataaatttt ttcggaaaaa ctaactaatt
     5521 cattccacga tttatcctcg gcaaattgac ttagtttcga agcgataaaa ggaaattttg
     5581 cttttaagtc atccatattc ttgatctcaa attcgatatt ttcttctcct acagctgaaa
     5641 tccatgctga aagattgatt gcaatatcgc taaagtcttc tacattttcg acatttcctg
     5701 tattatcttt tattactgct tctaaagcca tatcggttgt tgcactcatg gtttgatctc
     5761 ctctcataat agagcaaaaa gccggcttaa ccgacttttt gcatggtacg ttttgtttgg
     5821 tagttttctg cttgtgttta tgttgaaata atccctatag ctcttctttt aatagttctt
     5881 caactttatc tcttaactct ccctgaccac tctctaatag atttaaatag ctcatcagaa
     5941 tatccgtttc atacgttttt aattcagctc tatactcact ttctgaatat gatcgtaaat
     6001 acccatcatt gctaaatgca actagatcat cttgaaagtt ccattctcct aaatttgttt
     6061 tactcactgc ttcccatgga ctattactaa acatttcatt aaagaaatct tcgtcatttt
     6121 gatatacctt caattcagga aaatagttgc tgtcttcatt gatttcttgc accatttttc
     6181 caagttcttc ttcagataat ttaccttcca ataattttac tttttctctg tcatttgcat
     6241 aagccatttg aacctctcct ttttcttttt caatttcttc aggtgtttgt tttggcgtaa
     6301 ctttcaaatc tattccattg cctattagac ctattagata accatagaac gttttaaagt
     6361 cacttgtagc aagagatttg gcatcagggt atgtatcccc tccaatttta tctaaaacaa
     6421 aatcagcaat cgttacccaa ggagaatata tcgtacacac tacatcccat gtagcatctt
     6481 caatattcgt ccatgtcgga aagtcatttt cattcaaata gtcttttttc attgtttcga
     6541 tcagagtttt tccactttcg ttttggctta gatattcaac aaattctgca ctatcaaacc
     6601 tgctactaaa tagtttagct aatgagtcta aagctgtttt tgatattccc accattacta
     6661 cacctcctat taatatttta cgatgacttg tttttcattc acaaataaga ttttctacat
     6721 cttatctatt tctttatagc ttttccatac attatttcta aatacaacca ataaccctat
     6781 taaattaagc ttttgagttt atcttttcta ataatattct gataaatatt ctgataaaaa
     6841 aatttgttgt atttttttct tgactttttc tttagaatta agatcagttt tgattttatt
     6901 taaaagtgaa tctagctcta ttcaaaataa aaaagagatc aataatagta tcaatccctt
     6961 tttactgtac tctatcaatt tttataatct tcattatgct tttcgaaact ctttgattaa
     7021 aagaataaga acatttagac ttgctaatgc tcctattaca gtccaaatga ctaaaagcat
     7081 tttctgatgt ttattggtaa ttcctgtgaa tattgaactt gccttttcag caaatgttct
     7141 gtcttgcatc tctacgctac cttgttccgg aatattaaga tcattatttc catctaattc
     7201 cttgttggca cgatcaacat cctcaaataa atcattagaa gatgtaacag tttctgtctt
     7261 atctgaaacg tactcgctat caatggcatc agcgtatgcg gtattgccaa ctgttacttg
     7321 tggcgtgatg tattttgctt tttcatctct tagacttcta actaataaac gatcgtagtt
     7381 ctgaggatat ggatgacaag tataaagtgt taacatatct aaatcaggaa tatcagtcag
     7441 tgttgaattc tgaccgtaat ttctaataac gtctgttcgg aaaaccgtat aaactgcttt
     7501 ttcaccaaga taatctatat aaatgtggtc tccggctact aattgatcaa cataaagaaa
     7561 ttgtggtgac gtataaccgc cacgatgacc tgctattact gtttgatgac ctttacctga
     7621 taaaggtaga ctagtagcac tcatctgtgc cacacctcta tataaatgtt cctgtgaagc
     7681 gcctagaaac aaaagctcta gcacatttat ttttggaata tagacataac caaccgcccc
     7741 tgaataggta gatggttcaa aatctttcgt ttgatttttt gaaaaaacat ctcctgtgta
     7801 aggattgacc tttccttcag cttcaccaat atcaaatgat ccgccgtctg ttttctgagt
     7861 attgtctaat ccttggttac cgctgttcga ttttatttgt tcgacttttt ttgccacttc
     7921 ctcttttgaa ggagtttgtt ttttctcaat tttctcttgg atttccgcca cattctcttt
     7981 tgttttgaat tgatgataga gctgatttgc aggataaatt gttaaaacca aagtgcaaat
     8041 aagagtcaaa aaaagtagta ttttattctt catttttcat tcctcgtctc tttgctacta
     8101 tttttttaat aatgactatc acagctatta ttaataaaac ccctacaact acgataggat
     8161 accagttcat ctgcgccttt acctttaaat cttctttctt cttttctttc tcaacaatac
     8221 ttttttggta aggtatgcta acccctctaa cgagtaacct atgagaatta tctccaacag
     8281 gtgtacatgt cataagtgtt acataatctt ttcctgccac aggaagcaaa tcctcaaatt
     8341 cggttggtag gattattttt atccgatcta cttgataagc aagctttttc cctgaaatat
     8401 caataaaaaa tttatcacca agtttgagca actctaaatc tgtaaaatat tttttattag
     8461 gaatgcccga atgagctgat ataacagaat ggtttccaat cccaccagtt ggataatcag
     8521 ttccggttaa tattcctgct cctgctgata gttgaaaatc gtttacacca tcataaattg
     8581 gcaaatccac attaatcttt ggtataagaa tttcacccac taattcacct aatttatcct
     8641 tctgaagatt tccatttgac ggaatattgt tattatcttc aggaatcgtg actgaggttt
     8701 gttctgtgtc ttttcgtttc ctattaactt ctgaatatct cttgaagact tcttcattct
     8761 ccttttcaga agcttcatca tatttttcct tataggagtt tagtgtttgt tgagtttggt
     8821 agtaggtata agcctgagta attacaggat atagacctac tcctaaacat agcaacatcc
     8881 caatttttaa gatgatcaac ttgatattct tctttttcat gctcatacct acttttcaca
     8941 cactttttaa aaagaaaccc tagagccaaa aacactctag ggaattttat ttcattaagc
     9001 tttgtgttgt tctcgtttgt agacaacata tagccctgtt cctgccacac ctgcaaccac
     9061 tagaaatact actgttccaa ttccacctgt acgaactgtt tcaggtgttt taaggttctt
     9121 gatgtctgtt acttcgctag aagcatcatc attcgatacc acaaatggaa catcatttct
     9181 agttggtgta tatcctgaag gagcaacaac ttcttcaaga gcataatcat tgattttttt
     9241 atctgtgatt gttggattac cttgtttatc aaaatctaca tcataggtat attgcaagcc
     9301 ttcaatttgg aacttaccgt ctgatccgga aacgaattca gtagctttag atttatctgc
     9361 aatccaagta acagctttcg ttgttggatc ttgttttagg taagtaactt tattcggatc
     9421 aattttgttc ttcactacaa attttgctcc actcaatgct ttgcttgtat ctccatctgt
     9481 tttaatgaat tggtgatcac caatgattgt ataagcgtga tcaactggtg tttcgtcttc
     9541 gggttcatat ccattgttgt agatcgttga aacatcattc ttaagtggtg tatccatttt
     9601 agctttacta ttagtatgag cgttcatcaa aattgtaaag aagcgatccc ctgcatcaaa
     9661 ttgtttctga agcttatcca tacctgattc agtaaagaca tagtttgtcg tttgtccgtc
     9721 acgttcgata ctgtaatcag tatctaaagt cattccttcg atcacttctg atgaatcata
     9781 agttaactct gcacctagaa catctttgac aacatatttt tcgtaattac caatattacc
     9841 aggaattgat ggtttcaatg tatattgaac atcttcgcca gtatcaacgg tttctttatc
     9901 aattacctta gtaattacag gtcgtttgta gttggtaaca tcaagaacta ctttagcagt
     9961 ctccccacgt ttaacgctaa atttgaattt tttaccatct agtaaatagg ttgttttatt
    10021 agcatctgtt cccaagtcgg tttctaggaa ataatagtca cctaattcca aaccatctac
    10081 agttaattta cctgtgctgt ctgttttata agttccaaca acctgatcag atccatcatc
    10141 ttttcgcaat tcaaaaacag ttccggcaag agcctttcca tcttcgtctt ttttatcaag
    10201 ctctgctgag tttaattgga aatagttttt aggatagaca tgtacatcat aattaaagta
    10261 ttgatcatct gatccgttta gttcaggtac gttaacaatc ataggatacg cttcttcaac
    10321 tacacctttg actgttcctg tttcaacaat caaatacaag ttatctgtta aatcgtctgt
    10381 atttacttgt ataggtatgt ctatattccc ttgtgaatca gtcttcattc tacttacttc
    10441 actgtatttt ttgtttagta ataatcgtgc tttagcatca tcaatatgtt gaccttttgt
    10501 agtatcacta tccgtccatc ccaaggattt agcttcatct ttagtaagtt tcacataaga
    10561 gaactcaacg ccttcaagag gttttacacc atttggtaaa gctatttctt tccctttgtt
    10621 atcaatatct gagacagggt tcccatcccc attagtattt gttactttat ggatatgaat
    10681 cgttccagtt ccttgataat tatagttttg ttgtgcatat gctacttgcc cccctagtcc
    10741 aaggctagaa gagattgagc ttgcgcccaa tcccactcct agcaaagtac caaatagcat
    10801 taaatttgaa aaaatttttt ttgatttcat acgtcagcct tcttttttca tatttacttc
    10861 ttttccatta acactttttt attttgcttt gtgctgttct cgataataca gtagcaccat
    10921 actcaatcct gcaattagaa atacaagaag gaaggctgat gtaccgattc cgcctgttct
    10981 tactgtttca ggtacaaggt gattaattac tgtatattca aaggtttgtt catcgtcagc
    11041 aacggtgatg ccttctttag accatttccc tgtataacca ttagggtttt gtgtttcttc
    11101 gattttgtac gttttgttgg caacaatatt aaatgttgcc aagccattgc tgtccgtttt
    11161 ttgtacaact tttacacctt tgtcgtctgt caaagtgaat tctgcgtttg ctaatgcttt
    11221 cccttgatca tccactttat gaactttgat cacacctttt ttcaaagtgt ttttagcagt
    11281 atattcaaag acttgtccat catctttgac ggtaattcct tcttgtttga atgcgcctgt
    11341 gtaacctgtt ggtacttttg tttcttctaa gttgtaagtt ttgttcgcaa taatgttgaa
    11401 cgtagccaga ccagttgcat ccgtcttttt aacttctttt acaccttttt catccgttaa
    11461 ggtgaattct gcatctgcta aaggttttcc atcttgatcg actttatgaa cttttactac
    11521 accttttttc aaattatttg tagctgtata ttcaaaagtt tgcccatcgt ctttaacggt
    11581 gataccctct tgcttaaatg cacctgtgta acctactggt actccagttt cttctaggct
    11641 atacgtcttg tttgctacaa tattgaagct tgcaagtcca ttttcatctg ttttttctac
    11701 ttgtgttgca cctttttcat cggttaaagt gaactctgca tttgccaaag gttttccatc
    11761 ttgatcgact ttatgaactt ttactaaacc ttttttcaaa gtgtttgtag ctgtatattc
    11821 aaaagtttgt ccatcatctt taacggtaat tccttcttgc ttgaatacgc ctgtataacc
    11881 agttggtact ccggtctctt caaggctgta ggtcttgttt gctacaatat cgaagcttgc
    11941 aagtccgttt tcatctgttt tttctgcttg tgtttgggat gaatcatccg tcaaggtaaa
    12001 ttcagcatta gctaatggtt ttccatcttg gtcaaccttt ttaactttta ctttaccttt
    12061 tttaattgtg ttcgtagcag tgtaactaaa ttcttgtcca tcggtattaa cagtaatacc
    12121 atccttttta aatgaacctt gataaccttc aggaactttt gtttcttcta agctgtatgt
    12181 taagccttgt tgcaaatcaa acgtagctaa cccatcatct ccggttgtct taacagattt
    12241 agcattgtta ttgtcagtta gagtaaactc cgctcctttt aaagcatttc catcttgatc
    12301 aactttctta actgttacct gaccaacagc tcttgtccat ttagcactca aagatttaga
    12361 gtcctttgtt tctgaagtag tgatcataac tacggcatct tgatacacac ctcgtccatc
    12421 aaaagtaaaa ggattggttg tgctaacatt agcagtacct ttaaatgaca aagtaccact
    12481 tggtgtagtc acaggtactt taatagtaaa acttgaattt aatttcacag tgttgttagt
    12541 aatttcttta ccatctgcat cataaatctt cataccagct ggttttgctg atgtaaaagt
    12601 gagcttcgca ttaccagtct tatttgatgc tgttttatat gtgaaagtgt gatattttcc
    12661 ttcgtcagct gtcttagaag ctgtgataga aaactcagtg ttgggcgtat cttttccatt
    12721 cttagcatag tcataaattt tttcaaatgc tttttttaca cgagctgttt ctgcggcggt
    12781 atggtttgga tgtgaccata caatctgtga aactttaaag tttccggcta aaacccatga
    12841 aactaattgt gttgcatacc atgcttctgt atcatttcct gcacccagtt cctttgctgt
    12901 ttttgatgga aaaccacctt taaacatacg atagaactca tcactgccag ctcccttgta
    12961 cttcaagctt ccggctccac ttcctgttgg ggatggcaat tctgaatcca tacaatatcc
    13021 aatatttcca tgagaatctt taaattgact tgaaatcatc cattcatttg gatgattcgg
    13081 catattaacc ctaatataac cgtctgtttt tacgccagtt tctgtaaatt ttgcacttgt
    13141 cggttttgtg acatctgctt cagctcttat tgctgaaaaa ggcattataa gcgtagataa
    13201 caccattact aaagtcgcaa aacaataaat tatttttgat tttaaaatta tcgttttttc
    13261 atgcttcatc tctcgttact ccttctttat accagttagg cgaagaaaat gagccacgat
    13321 atgtcggtgt ggtaatgacc ttcgcgccca ttcctaagtt aaaatcacca cacctattaa
    13381 tcactttttt cgttgaaagc aagctgtttc aacgctctag tattcatagc atcacccctt
    13441 ttagttctac caaccatgag caacaagttt tctaagtagt taagcaagga caaataaaga
    13501 ctatacttaa ctacttagta tataaacgat ctcttttatt aacgtaccga aaccgctaat
    13561 tgagatacgt tatatgctgt ttcaaatgat cctgcattaa tccaacttgc cccaaacaca
    13621 ggatcgacaa catggtaaaa accgtcttta aatccatcaa cggctactac atgtcgattc
    13681 caaatagcat cgccccaaaa cattgattta tgttgagcag gttcaaaggc ataagttacc
    13741 cacgctacta caggatttcc atttacaatc tcatctttta atccttgtac cgatgaacca
    13801 gaaatatcta caacatttcc gccattagca acaccccatg aaactacagg agccggattc
    13861 ataccttgat aagttccatc cacattgtgt ctccattcac cagaaaaacc attatttgga
    13921 ttattatcag gagatagtgg cattgattta acaaaagtca ttaaatcttg atttgttgca
    13981 acacctttat aatgtaacgc ttctagcaaa gatgttcctt cgcaaagcat tgtgttacct
    14041 tgactaataa atggaacgcc tagcacaaca ttagttggtt gttcgggttt acttggtgaa
    14101 gtagaactgt cagtttcatc acttgaacct gtttgtgatt gatcactaga agatgtactg
    14161 gttgattcat ttgcatcatt tgatgttgat tgtgagttat ttgttgatgt tccagtatca
    14221 gcttccgtag tgctactttc acttgttccc tgtgatgctt ctttctcact tgtagaatta
    14281 tttgtattag atgaacttgc tccatcaaca cttgaagcac ttgtttctgt cttatcagaa
    14341 gtgtttgctg ttggttccga ttcttttgta gtctcggatt ttttgttctc ttgctccgta
    14401 ttttcaacag gatcaggttt atctttttga acagtttgac taactacttc gccatctgct
    14461 tccttaactg ttaatgtatc tttattgatg ttgaaagttc gtccaatcgg gatagtccgt
    14521 tgttctcctt ttgccaaatc aacattgttc aattcggcaa actcagcaac agtagtcttg
    14581 taacctaagc ttttaagctt ctctacagtt gaccaaatag tatctccttt tttgaacgca
    14641 taacttgatt gtcctgttgt aatttggatt tgatccggtg tgtttgcagt ccattcatca
    14701 gcatgtgagg ttactggtac tgataacccc gcaagtacag ctgatgcaaa taaaaccata
    14761 gcaagcgaat ttttcttcat attcattcct tctttttgtt tttattttct gtttgtgatc
    14821 gcaatgcttc tattccttct tccgttataa tataggtttt gtcacccact ttcttgaacg
    14881 aacctttagg caaacgattt ggataatagt tgattatgtt tgtaacataa tttctacctt
    14941 tacccaaaat tagacttgct tcccttgcgg tatacaagtt gtatttgtct aggttcaatt
    15001 tctttttatt tgcgatttag acctacccct ttcgcaaaat ttattttata ataatagtac
    15061 cattgcacta tcataattgc aataattttt tggggtttat cttcatttaa tagcctaaaa
    15121 aggcttatta gataaggaat atctaactaa tcaaaacaaa aaaggaagtt aagaagaaat
    15181 gaacaaaaaa aggaaattat cagtattaat tacacttgtt tctatgttgg ctattcttag
    15241 tgggatcttg tcgattaaaa ttttcaatca aataaataca agcgcaaata ccaaaaacga
    15301 aatagtcata gaaaatacat ctaattctaa agaaattagt actactaaaa acaaagacaa
    15361 atctgatgaa accactacta aaaattatct aataaagact gtttattttg atgaacggaa
    15421 aacagcaatt ctcaaagatg aggaaggatc agaattaaag ctacctataa cggtatccga
    15481 taagttcaat ggaacgttct ccttacaaac cgctgaagga tcacctgata atctcaaagt
    15541 tgggcaaaca atcattcttg gtaaagacga agaactgtat tcatttgtaa aataaaaaaa
    15601 tggagcaaag gctagtcaag cccttgctcc attttccatt cttcaagagg tgtagctatt
    15661 tcaacaccaa agtttaaata ctgtggatgc tgtagataca tgatattcat tctattttcg
    15721 ataaactctt ttaacgttga gtaaatatat ttttctaatg ctatatctgc ccaactatca
    15781 taatcaagtc tattggtgat attgacgttt atttcaattt gagaaacatc aatattttga
    15841 acatcaggaa acttattcag tcttagctct tctctaaccg cttctactag cggttcatat
    15901 tgtccgtctt gttcgctaat tgttgaaact atttcatctg aattccaacg atcttccaat
    15961 acctttgcta cattttcttt aggcggttgt ctttctataa attcttcagg tgtttcatca
    16021 aactctactc taaagttcaa tttattttga ttgttagcgt acatataatc atctctgttt
    16081 gattcaaaaa agttacgaat tcctgtatct gatttatcac ttaactcctt atttgctaag
    16141 ttagcccaaa aatcccaagt tcctctattt tcagtataca aagtaaaatt aatgtttata
    16201 taatttgcag tttcaggata cccttgctct ctaagagcat ctttaacaat caccgctaag
    16261 tcagtttcaa aattctccat aaaaaattca ctaaattctt ttggatccca cgtttcttct
    16321 aataactttt ctaatttacg atctgccatt attattctcc cccactttac tttcttatat
    16381 ctaaaactgc ttaaaattaa gagctataac ttatcataga aggtttttta tgatttattt
    16441 aagtctacaa ttctatattc taattatcat ctagaccatt ttcttatgtc aaataaacca
    16501 tatatattgt ctttaatagt tttatttgac tttttaagaa agtatttact tttgactttt
    16561 tttacgtttt acaacatata cgtattgtaa tgttcagctc aatttattat aatcaaaatg
    16621 aattgattaa taaaaattct gaggttcggg ttttttatca attcttttct gtttgtgttt
    16681 gtgatgaaac aatccttttt cggtaggttt tgttatcatc gtcagcacaa agagctaacg
    16741 ttggtaccgt tagctctttt tttattttac tctgctctta catagctatc taaatcctcc
    16801 aagtaaaaca attgattttc tcctatgctt gttactccac gccaaattgt tgttgttgct
    16861 gttgttggag tatttcttgt ctgtgtaatc aattcaacca ttttaccttt ttgatttgtt
    16921 gtatagtaat cagcagtata gctagatatt ggactattac cagtacctac cattgtgcca
    16981 gagatatcaa caggactaga ctttactatc tgaggaaatt ctttttgtat ttccttcata
    17041 ttcttcgtat atttttccca tgaattccag ttgtaaaata aattaaagaa tttcctactt
    17101 tgttccttga tttcagcatt tcccaattct ttgtattggg cttcttgcat agctttcact
    17161 ttctggttat tcttgactac ttcgatttgt ttacttttaa ggtcttccaa ttctttgaca
    17221 ttattatcat aagcattttt cttttgaaat agtaccacac aacaaaggat caaaattatg
    17281 gaaagtattc cgatcgcctt tttagcagta gtaatttcat gattttgcat aaaaagcacc
    17341 cccagtcact ttcttatcat tatttagtgt gatggttaat gtggtatcat acactttttt
    17401 tccgttgtat tgaacttcga aatcagtcaa tgcttcatac aacccactac tagatgtctt
    17461 aattccaaag tttgttagct taaaagtaaa aggtttatag tattctattt tttcttgctc
    17521 gctactctcg ttagtaactg gtggcttacc tgtcacaata aataactttc gcaaaaattc
    17581 ttcacctgac tgattcgcaa atttgcttcg gtctgatcct ttaaacatct cttgaatatc
    17641 attttctaaa tctctttgca atccatccac ttcatttggt ttaacctctt taaagaaggt
    17701 caattcattt tcaatttgca cactgtgctt ttcagcttct atgtcttgat tctgcttatt
    17761 gaaatataga attccaacaa gcattgctaa tagcatacca gaaagcaaag aaatgataat
    17821 tgcatctttc gttttactat tcatctctgt cgccctccat tcaaattttt atttttcaat
    17881 ttcaaattta gctttgccta caccgctaaa gaaatgcaca ttatctgacc catccacacc
    17941 atcaatcaaa gttactgaaa cacctcttgt tggtgcttgg gtaggtggaa cattgaaatt
    18001 agcgcacaaa aatgatccat cgtcaaacac cgctataact acacctgtat gtcctgctga
    18061 tccaacagcc cataaccatc catctaaagc actaaacgca taacctacaa ttggtttgtt
    18121 cgtcaccttt gctcctgccg atttgtagct ttcccaaacg taaccgccat ttcccaaagt
    18181 tggttgattt ccatcatata attgactcat ataagcccat gttaattcag tacattgtcc
    18241 ttgtaattca ccttgaaaag gtgatgttga ccagtctggt tctttaaatt ttggtagcgt
    18301 gattgctttc ccatattttt ttaatgcttc ttcataagtc atatttgcta ttacttgacc
    18361 ttttttaccg ttaatttttc cttctttccc aattgatcca gaaccagaat tagcatcatc
    18421 cgtagttgca caggcatctt ctactgttcc tgatgctgaa cttgatgaat tattcgatcc
    18481 atctccggta cttgaacctc cggatatttt attgatctta ctttcatctt ttgcaccgtc
    18541 catacccttg cttttcatgt acttccagat ttcttttgca ttttcaccac gtacagctat
    18601 tacagctgat tcggaatcag ctgaaatttc ccagttttta tgaaaagtta ctgcattttc
    18661 tactacatca tcagaagcat ttaaagcata atcttttaat attcctacga atcccccatc
    18721 acctttgaac ataaagtcca attgggcttc tgtagtccac caagaaccgc cattattttt
    18781 cttagaccaa tcaactaatg ctgtatgtcg cccggctgtc cattgaccat agccaatacc
    18841 cattagacca tcagtaaatt gtttgatgaa gtcaacttct ctctgcccat cgccgtaata
    18901 cccttctact gttcgaggat caatacctga ttcttgtttc cagttaccta aaatacctgc
    18961 aatactagca cctgataagc catattctcc attcatgtat ttgtaaatat cttcaatatt
    19021 tttggctaca tcacctgtta cagcgccact tccagtatcg tttccatcgc tatctgttcc
    19081 tgacccacta ccttcagtag tacagttaaa ttgtgcatca taagtagcta tcccatcagc
    19141 atgtaacact tgcggattta ttgtgattac actcattgca atccctgtaa actgcaccat
    19201 taacgtaagc aacgtggcaa tctttctctt agattttttc aataactacg cccccaaaaa
    19261 gtgtactttt ttatgttatt atgataatat aataacataa attaatagaa aggaaaagct
    19321 aataaatgac tgataaaaca agtgatttac ctaaactaga tccaaaaata tctcaagtta
    19381 agagcaaaaa agatggaaaa actaaaaatg ctgtaaagga aacatcctcg ttactacttg
    19441 atattccaat gagttctttt gatgaaatac ttgatgccaa tacggatgaa acttctgtta
    19501 aaattgcatc tcacgttgga atgaacagtc aaaatgatta tttttacaac ttgttaaaca
    19561 gcctaagaaa gttggaagat aaaaaaaatc aggtaatcgc ttcaaaagct cctgacctca
    19621 caaccgattt ggatgctgaa attatccaaa tcaaacaaga tgagcgatca gttaaatcgc
    19681 tactgaaaac ctactttgat ggttttgata aagaactagc tccttatagc caagcaatcc
    19741 taactaaacc aatacaagct ccacatattt caatttccct acaaaaaaag agtgatgact
    19801 aaacagtcat tactcttttt tagatttctg gtaagcgctg atcgcaaaaa ccatcaacaa
    19861 tattgactca aaaaatatgt tcattctttt ctcttttttc cattatagat ttccctctat
    19921 tccgttaaat tgaaagtcac accgattact acaaagatta acatgaccac attcaaaaac
    19981 attaaaagat atgctgataa tctcatcatt tttttagttc acttactggt aatctaaacc
    20041 tttgagtgat tgcactggaa ataccatatt caccaattac aagcaaaaaa tacgcaataa
    20101 tttcagttcc acctagatag atcagtaaaa taagtaagtc acttaacatt gttagtttcc
    20161 tcccagtata tgattttcat ttttgggaag tcttttataa tccaattcct caaaaaaagg
    20221 aaccatagtg tcactaatat aggttttagt ttcaggtact gccaaaattc cccaatcaag
    20281 aatttcttcc catgatacat catcaaattc taaaatatta tgatcaactg acctattacc
    20341 aagttcatat ccaccaaaaa ttaccatatt atcaatcatg tatagaattt catccggcga
    20401 accatgagtt aatgtttcgt cctcttcaag cagttcgttt aatctttgtt tgtattcttc
    20461 tcttttcata cttttatccc ttcttactca tttttttgaa ttttaacatt tttaatccaa
    20521 gtctgcttgg tatctctgta gtagtcatgc gcttttattg tcgccttaac ttttcttatt
    20581 tctccaacat cccattcatc ataataaaga aatttatttg tcttccaaat caatgaatta
    20641 tggttttgat cttcaaataa atgcaggtaa acattgcccc attgtgtatc tatggtgacg
    20701 gtattggtaa gtttcaaatc caattccaat cgttgagcga ttgtaccaat aaactcaccc
    20761 tcttctctac gcttcgcact taaaatctcc tgttgtcttt tgctgtattc ttctagcttc
    20821 aactcaataa ttccttcatc attgattaca gaaaattctt ctacaaaaac tttttgagtt
    20881 ttgtattgtt cttgtttttc cttaaaaaaa ccaacctaat aaaggatcat aagtagctcc
    20941 ttgatgatgc agttcatctt tgatcgtata gctatttttc tctataacca cgtaaatata
    21001 acccttttca aagccataac gatcaaaatt tttctgagat tctttctctc ttgcgatcct
    21061 tatttttcga tcagctcttt ttttatttaa aacatttagc ttagttctat attctggcgt
    21121 aaaggatttt tcagttacaa caagaaatcc atcaccatcg cacttaaaac atgtgccata
    21181 ggtaattttc cccatagaca taaacttgta ctgacctgat ccgctacaac gatagcaagg
    21241 gatttttttc ttcatgatta tagttccatt tttatccggt tgcgatttgc tgatgatttc
    21301 ttcttgactg aacatcctcc atactccttt ctgagcgagg ataaaacctt gctcatcttc
    21361 aggttgttta aaaactagga tcgtagtatt tttctctatc acctatataa aaatgaatat
    21421 cttctttaag cactttccat ccattttttg tcttaactaa ctccaatatt tgaccttcag
    21481 gatttctgct atattcccac tcttgatctg gaaaagtagc tttattgatt gctccatccg
    21541 cttgaaccaa aattctgttt ttgcttttta cttcaattac tgtataagga tttctgtcgg
    21601 tatatagaag ctcggttgct cccattccaa ctacaatctc cttgtttgtt gtactgcctt
    21661 ccatcattct attttgtaag ctaccttcta atttcattta agatcaccct ttctcaactt
    21721 atctacaaat tcaccataca taccgataca tattttgtaa ataaattaat tttataaata
    21781 gataaaaaac gatgcctatt tagcatcgtt tcatttgtta atgtttaatt ataaaatcgg
    21841 actttaggag aatgatctgg aaaaatagac tctgtttcta tttcttggat gagagaaact
    21901 ctctcttcca tcaattgttt atactttgat gattctataa tttcacttct agatttattt
    21961 aaacacctca tagattcata ataactaaac attctatttc tatttctatt ttcatttaac
    22021 agttccaatc tttcctgatt tcttttctta ataccttcta tttctttcaa ataatttatc
    22081 cccttccata ccaattatga tgccacattg gatgaaataa tcacatacta tttttgacca
    22141 aataactggt caatttagaa ttatttacac caaatatcct tcagattttt tactacttca
    22201 taatattctt gtgagtcttg aacctctttt accgctacaa ccgctgtcaa gtctttagga
    22261 tgaaatctaa gtgccgatgg ttttatcata ctccatcgag tattttctaa tcttccctta
    22321 gtttcaagta aagctacttg aatgatattg tctcttttat tgtagtaaaa agtaaacttt
    22381 tctgggaact taatcattga gtttaactct tcaaaccatt cttttgatcc aaattcaact
    22441 tctaccattt tcctatctcc tttaatttca tttttcgtct attgttaatt aattgcattg
    22501 cgttccttta tactagttag ataatcgttt atttcagcta tatttttgtc tgcataccat
    22561 tttggataaa gcatgtaagc atgactatct ttacatgcta ttaattttta tctccatttt
    22621 gatatttctg cttttttcta ctcaccaatc aaccgtagat cctcaacatt ctcaccttca
    22681 agcgaatatg agtatacttc gccgtaaata ttttctataa ctacgacttc ttcttcaaag
    22741 tctatggact gcacatcaaa ccattctcct ccgccaaatt cgtctatgat taatacctgc
    22801 aatattttat ttactgccat ttcaatttcc ccttatacca ttctatgtct tccaaactat
    22861 ttttcttctt tccataccaa ggtttcacgt atctcaaaat tttctttaaa taagtgttcc
    22921 ggtactttta taattaactc ttttcctact tttatcagat agtctttttc acctaatgag
    22981 atttgaccag tcggtaactg tatcgaaact cttttagttt tattaaattt tttagttttc
    23041 aatacagtat acggtctaca gaatttctct aagcgttcaa atcttttact atcgtattgg
    23101 aaataagctt taggaacaga taaagtacaa aaactatcgg taacagcact tatgtctttt
    23161 ttagaaatta tcccaacttt agaacttcta aggaaagttg ctaatttctt atatccacca
    23221 tgacttgttt caatatagat gctattgtat tggctgtcgt ctatctcatt tattgtttca
    23281 acctttttcc tgacaggtgt ttcaacgagt tctcgaacct cttcttcact gtacgctaat
    23341 acaattaact cttccccatt taccatcttt agaacatacg cttgcttctt ttttcttggc
    23401 ttattttgag attcaacata accagacctg gaatctttat acttccaagc atgtactttt
    23461 ttctctaatt tctgtatctc ttttttcagc tgtagatatt cagacatttt ttcattgtaa
    23521 cgttcgcaat ataaacaata ttcatctttt ccaaaatcat gatcaagtcc ttcttcaata
    23581 tgtttattca acaaactaga aaaatattga ttatattgct tttttcgcaa tttaagctct
    23641 cgtaaatctt tagacatttc atttttcctt tccaattatt agtgccgttt cacaatttgt
    23701 aattgactaa acacaataat taatttgtaa gctaacacca attacaactg ttagtaattg
    23761 ggttagtctc tatcagatta tctggtgaat ttttttatta aacaaatttc atagctttca
    23821 aaaatcgctt tgcttgaaaa ggtgttattc tacaaacctt attcgccaac tttcaatctg
    23881 ataaagcaat ttgccaatga acaatgaact tcttaggaat cgttctatta ttaaacaatt
    23941 agtgaattgc tcaaagttct aatcactccc tctcttttat tatacacact aatgtgtata
    24001 atatcaataa ttttcttttc ttttttaata gaatatgtaa tctatcaaat attgatacaa
    24061 catttgtaac gatgctgttc gtgatcaaat tgttagcaat actcatttca acaaggaaat
    24121 atcgttctta gaaaaattct gcacttctta attatgaagc agtcatattt tattattatt
    24181 aaaattgttt agctatttaa ttgactagtg gctaaaatta tagagaccaa gtatttactt
    24241 ggtctcacat tctcttatat cattggttaa ttaactacct tcctttcaaa atatcaagaa
    24301 ttttttttgc tttttctttg tcttttgtat agaacaaact atgaatgttt ccatcaattc
    24361 cttcaaagtg aacagcagat tcttcatttt ttcgaaacca ttctgtcgat gagcttagca
    24421 catcatctgt agcaactctc ctataattcg tgtccataat tccatctttt acttttgtaa
    24481 ggtatatatc cccactaact tctgcgacta ctaattgtaa attttcgtat gccatatttt
    24541 cttacttctt tcttttttag ttgcttaatt ctactagata acattgatta ttcagagact
    24601 tcaagtaaca catctgacca tgtttcaagt tagttatctc tgattttagt gatgaaatat
    24661 agttcaccta aaaaacaatg ctccgaaaaa agctaattcg aactctatat ttttttgcag
    24721 tgtcatcaat ttttgtcaat tggactatta ccttcttttt cttccaaata gttttgtgca
    24781 agtttcctta cttttttcat catcaaattc gagagttttt gattatcttt ttcgctgtaa
    24841 aataatctac tcgttaaact tttaggataa tctttgatag aatatgcatc ccaaacgatt
    24901 ctttgaacat actcacgatc tatattttct ctgacttccc cagtccaaaa atgttgacca
    24961 taatggcatc tttctattac attgttagca gaaacttcaa aataagattg ttccctttgt
    25021 attatcatgc caaccatcat agcgcaggaa ataactatta ataacaagcc tgtttttaat
    25081 ttattattcc acatgcaaat tccacatcct tctctggata aaaatctcct tgacactttt
    25141 aaattacttt tttaaatata accaccaaaa caagatcatg ctgataaatc caagtgtata
    25201 caagataata aattctttgt taattatcca aaacactctg atttctttta gcttttcacg
    25261 cattagaaaa caccttgtta tatctttttt gtatttcttt caattgtttt ttaaaactca
    25321 taaacaactg ttcatgatct ggtctaagtg ttaaaatatt taaattattt atctttgtta
    25381 attgagtaat aaaatctatt ttagattttc tttcaatatc aaatccaaca ataatctttc
    25441 ctgttggatc aacacatttc acttgaatag ctttgtcatt ttgtgatatc tctaatattg
    25501 atgatttttc ttctgcttct tccaaaagat gttccaacac ttcgttaggt gttccatgat
    25561 agtaataaat catggtcttt tcgaaaattt ttatcggatc aacaccatat tcttttccga
    25621 ttcgataaaa aaatttcatt ggaatttctt ccggatttct gttcctgctt gaccaattag
    25681 agatggtttg ataatttgca aaatggattc gttcggctaa cctctgaact gtcgtctcta
    25741 aatctttttc tattaattgt tttacagcac caatgttttg tgacatttac tttctatccc
    25801 ttcaaattcg ttgcattatc aattctaaga tttcatgttt taacgcatgt ctgtctacat
    25861 tttgcttttt taactctgca ctataatatc ttcgaatccc tttatccaag catataattc
    25921 cttgatctaa tagttcatat tccgcttttt caagtcttct ctttgcctga tcaacaagca
    25981 aaatgatatt gtgatgtgtc tgattaaact gctgatcata ttcctctaaa gttttagcat
    26041 ctaagttagc aatatgatcc tctgctaatt tttgtgttaa aaataattcg actttctcag
    26101 attttaacat ctgtattttt tcatactctt gttttgcttg gatatagtca tctttcaagc
    26161 tagacatgta actcatctcc ttacctgtat aaactgatac ggactgtcca aaattgaata
    26221 gattggtgat gacttcaaga agacttcacc attttcacat gaaaataaac caattaattc
    26281 ttcaatttca tttacgatca gatattcatt agttaaaatt ttcccatttt ctttttctag
    26341 ttcgacaatc gcttgattcc atgtattttg cactaactct tgaatcaatt tcaatctatc
    26401 catttttttc atctcataca gggcaatgtc agcttgttta atgcctttta atttgcgaaa
    26461 ggcatattta tattgtgtgt agtttaaaaa gcctgtgtag ttatacatgg gctttcctga
    26521 tgccttgtat tgaattaagc ttgggtatat ttcagatttc cactttttct ctgaaaattc
    26581 gtcaataatt cttaactgat caggtaaatc aacatcgtag tatttttcaa ttattagttc
    26641 ccgatgaagt agatgtttct ccttttcttt ttggattaca aaagaagaat tttcactcag
    26701 tctacttatt tcttcccttg ttaacccaag tcttattttt tctacttcag ttttaagttc
    26761 acgaatactt tttgccttta accacttccc atttgcaccg ttattgtaac cggtaagtaa
    26821 ctgtatttta taaattcctt caccatgaag caccttatat ataccaacct gattcttttg
    26881 cggaatatga gcttcatttg cactgatttc tttcattacg cattctcctt tataatctag
    26941 ttaattagct tgtgtgtttc gagtagttga gtaagaacgt ttgtttcaga aatcatttat
    27001 ttgttatcac ttaattttaa aaaaataggg cttacgccct aaagaactat ttaactttgg
    27061 attgatcgtc cataacaggt tctgactgtt gatataattt atatgctccc aaatccatat
    27121 aatatttagt tgtactttca atcatttcat cagttgtatt tgttgtcatt cccattacat
    27181 cccagtatag tgtgccatta actttctttg gtgttactac tgcaactgtt ggcgtatgat
    27241 attgattgtt ctcatcagcc agaccaggaa tgttttgtat ccactcaggt acaccatcag
    27301 taatatctac agcaactgcg tgatactcgt aagctggttt atttatggta atatcatgca
    27361 ttaatgttgg agcataacta aatccttctt ttatttcttg catgattttt gattctgctt
    27421 tttgacaatc tggacaatca ggtttgtaaa gaacgatagt cagattttta ttttgcaatg
    27481 gatcattccc ataatcttca tcatttttta ctgtattaat aaagtcggta taaacttctt
    27541 ccggcttttg acctttatta atgtcttcgt atttttgata cttttcagca ttattttcag
    27601 tcattgaaga aattgttgtt gatgccgatg tagagctgtt tttagtaacc accgtactac
    27661 tagactttgt gtctttgtta ttatcagcac tattagttgt accacatgct gtaagagtta
    27721 ttaatgctgc tgttgataac aatacaatct tccatttagt tgatttcatt ttattcacct
    27781 atcctttttt cgtttgttta acactaaaag aataacccgt actatccaat aaaacaacag
    27841 catcaatatg gaaacccaaa ccatttgcac aatccaagca taaagactaa ataataggta
    27901 ttgtggattt ttttgaatga ttgtatttga gatgccattt cctacagtaa aatacattgt
    27961 tacgaaaata acaccttcaa ttatcaagtg atttgttggt tgattaataa tttttttcat
    28021 atttttactc ctaaactaaa tttattcttc ctgctaaata ttgaaaatta tatgggttct
    28081 gcacatattc cctaagttcc ttaatacttg tatcaatatc accaaaggct gttttaatgt
    28141 ttgctgaccc atctttatat acaatcattt ccccttcacc catacagaaa gaaatttcag
    28201 tattagaaat gattgttttt tcagcacgac cattgataat ttcttgacca tcacgccata
    28261 taaaatacat aagataaaat acctcataag cgttgtatcc agtatccgtt gttattgata
    28321 ccaaaacatc agcatcttgt attcctgata aagctaatcc aatttcatgg atcacatcaa
    28381 cttcattttc ttcccaatct ccactttcga agacggtata ggtttgattg ttagcaaatg
    28441 gttttctctc tatatcgaac attcctttcc aatcaagaat catgtacccg attaaattgt
    28501 gaaagaaaat taaataaaat aaatacaata agatgctaac tacaaaaata tcagccatta
    28561 ttgacatagg attatcagga tataaccaga tgtaaaacga ttgtttcaaa tccatttttc
    28621 catcatctcg ctccatatat ttatgctatc atgataacat aataacataa atttgtctcg
    28681 ctttttaata tttttttatg gtaaacttgt catacaaaca aaaaaaggag atttatatgt
    28741 ttatattaga tgatgaaata aaaaaagtta aaaatcctag aattattcca ttattagaag
    28801 aagttcgaag tagtttttat cagggaaatt atcgttctgc aatagctgtc aattacagtg
    28861 cgcttgtttt agatttaatt gataaattat ctgatttagc aaatatttac gaggatatta
    28921 cttctaagaa aattttagaa gatatagcta gattaagaaa aagtgatcca aattctccaa
    28981 aatgggaaat ggaactacta gaaaaagctt atagttcaac tgagctaatt gatagctatg
    29041 aatatgagga tttaaaatat attaaggatc aaagaaatta ttcagctcat cctgtagtca
    29101 cttacaacgg taatacttgg gaattaagag atataacaaa agaaacttgt caggatgtta
    29161 tacggaagtc ctatgaaatt atattcctaa aaaatccaat attaggaaaa aaagtaacga
    29221 tcgaaattat acaatatgca agtcgcatac atgctatgtc tgttacccct gaagaatttc
    29281 actctgcact aaaaaatagc tatctaaata aactaagcga aaaagcaaaa gaaaacctat
    29341 gtaagacagc ctttaagttt gtttttcaat taccttatgg aaatgaggaa gcagatagag
    29401 atagggaaag tactttcaga ctacttaatg ccctaatttt tgataataaa gaattctatt
    29461 taaaaattct aaaagaagaa atttcaaagt ataggttcac agctgaatca agagaggaaa
    29521 ttaataaatc acttggtgaa aatcaacaga tcacatatac aaaaggggtc tttttactta
    29581 cttttatagc tacccatcca gaattacaac atgatttttc agacgagaca attcaaaatt
    29641 tacaaatatc aacctgaata attcatagct ttaattcttt ggcacatttt attgaaatat
    29701 gtatcactaa tatccctatc aactgatttt gactaaaaag ttacttcaaa cattcactgt
    29761 tcaaaaatat tgaggaataa attttgggtg taaggcattt tgagcatact tctccctgga
    29821 taacttccca gtaaaaaaat cgttagattg ttggattaga tccattgact ggctcgcctc
    29881 aggcgagcaa agaccctgta gaattcattc tgatacacat gataactaga taatttgaga
    29941 tagactcgtc tacccgtttg gactaatttc ccagcaactt tcagaaactt caatcgaata
    30001 gaatgaatgg tcattccctt ttggacttct tcaaagccaa tcgttcttaa gaagttgact
    30061 aagttgtaag ctatcaagct gagcatcatt cggacatgat tctccaaaaa gcgaggactg
    30121 tctgttttgt caaagtaaaa gccagctttc gcttctttaa tgaagttctc cattgtgcca
    30181 cgtttggcat agatagaaaa gatggtatca ggagaaacat tttctgatag attcgtcacg
    30241 ataaattcat gtcgaaagag tagttcgccc gcttcacgtg ttgagcgtat acacacgcga
    30301 cgactttgtg accaggattg tgcttgataa gtggtcgaga agtactgaac ttctcgttct
    30361 tcccactttt gattatcgcc ataaagtact gatttctcag ctatttgacc tagtctacga
    30421 ttattcttca gtcgaataac ataatgactc ttttttgatt cacacgaatc atacacacca
    30481 ggtgtagcga acccgctgtc tccacgaacc aagatgtcag tgtttggtag agagtgatta
    30541 tagtgctcta ataaaggtgt gagaaactcc tttacgcctt ttgatgtata ttgatttcct
    30601 gaacgtagtt cagcttttaa gaagtcacca gtcaatccgt caaaagctac caatggatgg
    30661 taaccatagg tttggtagtg tgtattataa ttcgtttgtt cttggtgacc aaatgtatct
    30721 gaatgggtcg a
//
LOCUS       DAFGZE010000031        29349 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.031, whole genome shotgun sequence.
ACCESSION   DAFGZE010000031 DAFGZE010000000
VERSION     DAFGZE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.031"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            62..283
                     /locus_tag="LTU54_001497"
     CDS             62..283
                     /locus_tag="LTU54_001497"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296216.1"
                     /translation="MKQNKKTPSAKGALITLNFKDQKFSAVVDEIHPQEHQKNSFATD
                     EKKTQKRSEKVLRIIQFLLEKIRHFGSLF"
     gene            410..1186
                     /locus_tag="LTU54_001498"
     CDS             410..1186
                     /locus_tag="LTU54_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HBL8296217.1"
                     /translation="MNTPQIFNFEQHEVRTVTIHDEPFFVGKDVAKVLGYQNGSRDIN
                     RHVDVEDRQNYQNGTFESPRGLTIINESGLYSLILGSKQPNAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATKLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMRQGYTKSHTTEIPK
                     SDGGTKVVMNTKWTQKGRLFIYESLKKEGYIPEIDLLEEE"
     gene            1189..1377
                     /locus_tag="LTU54_001499"
     CDS             1189..1377
                     /locus_tag="LTU54_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8296218.1"
                     /translation="MSQEFILKVRIQLAKYGKSQNWLADTIGISRPYMSDIMNGRRKP
                     DKQIKPIEAALAELEKEK"
     gene            1379..1663
                     /locus_tag="LTU54_001500"
     CDS             1379..1663
                     /locus_tag="LTU54_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296219.1"
                     /translation="MQITIPDNLVVSELTTQITNAVLNSLDERLHLMNKSVELPPYPN
                     KSEVKKVLGIGDDKLTHWISLGLKTQQWSKLDIRIERSELQRFLKENFEF"
     gene            1684..1872
                     /locus_tag="LTU54_001501"
     CDS             1684..1872
                     /locus_tag="LTU54_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296220.1"
                     /translation="MSYTLQQEHQILGLIKQRRKQLQDDRAALRKSDELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2198..2437
                     /locus_tag="LTU54_001502"
     CDS             2198..2437
                     /locus_tag="LTU54_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296221.1"
                     /translation="MENELSTLDQYLADPDWGKSNLQLVANKYEKSIDEAIVALTDAA
                     SITADDSKWTKRDLGILKRGVNWLEDQYREETKEK"
     gene            2443..3129
                     /locus_tag="LTU54_001503"
     CDS             2443..3129
                     /locus_tag="LTU54_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296222.1"
                     /translation="MFKPLIDSYSAVLKKFKGNDIGATINEEVNIERLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            3132..3962
                     /locus_tag="LTU54_001504"
     CDS             3132..3962
                     /locus_tag="LTU54_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HBL8296223.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            3979..4830
                     /locus_tag="LTU54_001505"
     CDS             3979..4830
                     /locus_tag="LTU54_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8296224.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            4827..5186
                     /locus_tag="LTU54_001506"
     CDS             4827..5186
                     /locus_tag="LTU54_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HBL8296225.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            5201..5362
                     /locus_tag="LTU54_001507"
     CDS             5201..5362
                     /locus_tag="LTU54_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL8296226.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            5359..5664
                     /locus_tag="LTU54_001508"
     CDS             5359..5664
                     /locus_tag="LTU54_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296227.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            5664..6020
                     /locus_tag="LTU54_001509"
     CDS             5664..6020
                     /locus_tag="LTU54_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HBL8296228.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            6007..6225
                     /locus_tag="LTU54_001510"
     CDS             6007..6225
                     /locus_tag="LTU54_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296229.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            6246..6335
                     /locus_tag="LTU54_001511"
                     /pseudo
     CDS             6246..6335
                     /locus_tag="LTU54_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            6410..6877
                     /locus_tag="LTU54_001512"
     CDS             6410..6877
                     /locus_tag="LTU54_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HBL8296230.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            7152..7388
                     /locus_tag="LTU54_001513"
     CDS             7152..7388
                     /locus_tag="LTU54_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296231.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            7412..7753
                     /locus_tag="LTU54_001514"
     CDS             7412..7753
                     /locus_tag="LTU54_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296232.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(7746..7937)
                     /locus_tag="LTU54_001515"
     CDS             complement(7746..7937)
                     /locus_tag="LTU54_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBL8296233.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            8147..8794
                     /locus_tag="LTU54_001516"
     CDS             8147..8794
                     /locus_tag="LTU54_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296234.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            9054..9398
                     /locus_tag="LTU54_001517"
     CDS             9054..9398
                     /locus_tag="LTU54_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBL8296235.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            9403..9684
                     /locus_tag="LTU54_001518"
     CDS             9403..9684
                     /locus_tag="LTU54_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296236.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            9787..10101
                     /locus_tag="LTU54_001519"
     CDS             9787..10101
                     /locus_tag="LTU54_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBL8296237.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            10079..11773
                     /locus_tag="LTU54_001520"
     CDS             10079..11773
                     /locus_tag="LTU54_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBL8296238.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            11793..12971
                     /locus_tag="LTU54_001521"
     CDS             11793..12971
                     /locus_tag="LTU54_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBL8296239.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            12934..13620
                     /locus_tag="LTU54_001522"
     CDS             12934..13620
                     /locus_tag="LTU54_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBL8296240.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            13620..14780
                     /locus_tag="LTU54_001523"
     CDS             13620..14780
                     /locus_tag="LTU54_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBL8296241.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            14790..15665
                     /locus_tag="LTU54_001524"
     CDS             14790..15665
                     /locus_tag="LTU54_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296242.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            15662..15973
                     /locus_tag="LTU54_001525"
     CDS             15662..15973
                     /locus_tag="LTU54_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296243.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            15963..16316
                     /locus_tag="LTU54_001526"
     CDS             15963..16316
                     /locus_tag="LTU54_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBL8296244.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            16306..16707
                     /locus_tag="LTU54_001527"
     CDS             16306..16707
                     /locus_tag="LTU54_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296245.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            16700..17104
                     /locus_tag="LTU54_001528"
     CDS             16700..17104
                     /locus_tag="LTU54_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296246.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            17116..17721
                     /locus_tag="LTU54_001529"
     CDS             17116..17721
                     /locus_tag="LTU54_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8296247.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            17741..18103
                     /locus_tag="LTU54_001530"
     CDS             17741..18103
                     /locus_tag="LTU54_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296248.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            18106..18288
                     /locus_tag="LTU54_001531"
     CDS             18106..18288
                     /locus_tag="LTU54_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296249.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            18305..21724
                     /locus_tag="LTU54_001532"
     CDS             18305..21724
                     /locus_tag="LTU54_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL8296250.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            21775..22512
                     /locus_tag="LTU54_001533"
     CDS             21775..22512
                     /locus_tag="LTU54_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8296251.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            22522..24813
                     /locus_tag="LTU54_001534"
     CDS             22522..24813
                     /locus_tag="LTU54_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8296252.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            24837..26927
                     /locus_tag="LTU54_001535"
     CDS             24837..26927
                     /locus_tag="LTU54_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296253.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGKPNLMSKLKSSDFN
                     VGYHGSLTLDNEKLHFTSDGTGSIIMFTRINTPQLTSGKTYTLSAKVRFDEGTTGAID
                     KLRLVYRTSPGEKILLEANSTNITTDDVGKEITIKGTANVNYQITNLDRFYMSISFVD
                     RDKINGGFKLYDIKIEEGSTATPYQPNLLDAPYYLSKVALGENLIKPESQQPVTNSNY
                     LINTYNIKPMVKGKKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTNVWS
                     KTFTAADDSHPTTPQVQIYQVPSTSVGQCTIKWLKLEEGNTRTPNISEYKYRGTGMRD
                     SNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTT
                     AKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFV
                     ALNGKRSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNS
                     SYGSDYTFAITYTI"
     gene            26944..27093
                     /locus_tag="LTU54_001536"
     CDS             26944..27093
                     /locus_tag="LTU54_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296254.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            27090..27536
                     /locus_tag="LTU54_001537"
     CDS             27090..27536
                     /locus_tag="LTU54_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296255.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            27538..27675
                     /locus_tag="LTU54_001538"
     CDS             27538..27675
                     /locus_tag="LTU54_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL8296256.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            27713..28006
                     /locus_tag="LTU54_001539"
     CDS             27713..28006
                     /locus_tag="LTU54_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296257.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            28003..28227
                     /locus_tag="LTU54_001540"
     CDS             28003..28227
                     /locus_tag="LTU54_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL8296258.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            28224..29249
                     /locus_tag="LTU54_001541"
     CDS             28224..29249
                     /locus_tag="LTU54_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL8296259.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
BASE COUNT        10632 a         4410 c         6095 g         8212 t
ORIGIN      
        1 atgtcaataa aaattagcta attttattag cttacatatt taaagaaaag gaggaaacat
       61 catgaaacaa aataaaaaaa ctccttccgc aaaaggagct ttaataactt taaattttaa
      121 agaccaaaag tttagtgctg ttgttgatga aattcatcca caagagcatc aaaaaaattc
      181 ttttgcaact gacgagaaaa agactcagaa acgctctgag aaagttcttc gaataattca
      241 atttctgttg gaaaagattc gtcattttgg aagtctattt tgaattcttt attaacagtc
      301 ttgaatacgg tttgtttgaa taaggcgtcg acatccatat taaattcttc catactattt
      361 tcacctcgct ttctattcaa aattatatca aagaaaggaa tgagcaaaaa tgaacacacc
      421 gcaaattttt aactttgaac aacatgaagt aagaacagta acaatacatg atgaaccatt
      481 ttttgttgga aaagatgtgg ctaaagttct agggtaccaa aatggtagta gagatattaa
      541 cagacacgtc gatgtagaag atcggcaaaa ctaccaaaac ggtacttttg aatcgcctag
      601 agggttaact attattaacg aatcaggttt gtactcctta attttaggaa gcaaacaacc
      661 aaatgctaag aaatttaaac gttgggtgac tagcgaagtg ttgccagcaa ttagaaaaca
      721 tggaggttat ctaactccag aaaaagtaga agaagctttg cttaatccag atacaatcat
      781 ccaattagca actaagttga aagaagaacg tactggaaga ctaatcgcag aacaaaaaat
      841 tgcagaatac gaacccaaaa tttcgtattt ggatagtatc ttatcttcta cagattcagt
      901 aactattagt caaatcgcgg ctgattatgg gatgtctcca caacagatga acaaactact
      961 tcataagcta ggtattcaga aaaaagtagg caaccaatgg ttattatgca aaaaacacat
     1021 gagacagggt tacacaaaat ctcatacaac tgagattccg aaatctgatg gcggaacaaa
     1081 agttgtgatg aatacaaaat ggactcaaaa agggcgtctg ttcatttatg agtcgctgaa
     1141 aaaagaagga tatattccag aaatcgattt attagaggag gaatgacgat gtcacaagaa
     1201 tttattttga aagtcagaat tcaattagca aaatacggaa agtctcagaa ctggttagct
     1261 gatactatcg gaatttctag accatatatg tcggacatta tgaatgggcg tagaaaacca
     1321 gataaacaaa tcaagccaat tgaggcagca ctagcagagt tagagaagga gaaataacat
     1381 gcaaataaca attccagata atttagtagt ttccgaatta actacacaga ttacgaatgc
     1441 tgttcttaat tcattggacg aacgattaca ccttatgaac aaatcagtag agcttcctcc
     1501 atatccaaac aaatcagagg taaaaaaagt tttaggcatt ggtgatgaca aattaacaca
     1561 ttggataagc ctaggcttaa aaacacagca gtggagcaag ttagacatca gaattgaacg
     1621 atcggaactc caaagatttt tgaaagaaaa ctttgagttc taaaggcaaa ggagaatgat
     1681 tttatgtcct acacattgca acaagaacat caaattctag gtctgattaa acagcgtaga
     1741 aaacaattac aagatgatcg tgcagcgctt agaaaatccg atgagctatc agatagacaa
     1801 gctgaactaa ttgcttctga acttgaggat ttgagaatgc tagaaataaa aaatagggag
     1861 attagattat gaagaagaca gacacacttt ttataggatt cattttgggg ttattagtga
     1921 ttgtagcgca ccaaagtatt atcggaggaa gcttgttcgc agcattgatg gttttaatca
     1981 atctgcttga ctcaaaagaa aggagcaact atggcacgag aagaagcgct aaaaatcggt
     2041 aaagtgattg ctgataattg gtgggcaaat agccgttcta ttattttaag caagcaacat
     2101 atcgaaaaga aaaaagcatg gcaacaaata aaaaagtgac tccgccgacc aaagcaatga
     2161 gtcactcaaa aaatcataac taaggagatt ttaacatatg gaaaatgaac tttccactct
     2221 agatcaatat ttggccgatc ctgattgggg caaatcgaat ttacaactgg ttgctaacaa
     2281 gtacgaaaaa tcaattgatg aagcaattgt tgcattgact gatgcagctt ccataaccgc
     2341 tgatgacagt aaatggacca agagagactt gggcattcta aaacgaggcg ttaattggct
     2401 tgaagatcag tacagagaag aaacaaaaga gaagtgatat gagtgtttaa accactaata
     2461 gattcatact cggcggtact aaagaaattt aaaggtaacg acattggtgc aacaatcaac
     2521 gaagaagtaa atatcgaacg gctgaaaacg atgtatgacg gatatgatgg cgatcgagtc
     2581 attgaaattc gctttattga tccacgacgg ttcacagtac agcaaagaaa cttcatctat
     2641 gcgctcatag gcgatatttt catcgataca ggcatgccaa cggacttctg gaaggaattc
     2701 ttctacttcc gttttgaagg tgtcacaggg cgcgaaataa gcctcaaaga cgaatcgaat
     2761 acaaccgtga gtgatgccaa tatcttagcg aatatcatcc tagatttcat ctttgaacat
     2821 catattcctt tcaaagaagg atatgagatt ttaccagcga atcaagagta ttacttctac
     2881 aaatgcatta caaaaagagt ctgctgcatc tgtggcaaaa caggagctga tatcgatcac
     2941 tttgacaaag cgctgggaag acgaaagcgc aaagaagttg atcattcaga gtacacattt
     3001 gcagcactct gcagaatcca tcacacagag aaacacaaaa taggtgtgat caatttcaaa
     3061 aataaatatc aaatcaaagg gatcaagtta aaccaggaaa caattaaaaa gttaaggata
     3121 ggaggataaa tttggctgag ataagttgga tcaaacttaa aactactatg tttgacgatg
     3181 aaaaaatacg attaatccaa gctgttcctg agtcggatgc catcatcgtt atatggattc
     3241 gattactagt tttagcagga aagactaacg acgatggtct gatatatatc cagaggaaca
     3301 tgccttatac cgaagaaatg cttgctacat tgtttggcaa aaacgtaaat acggttcgct
     3361 tagcgctaac tacattggca aatttcaaca tgattgatct aagcagtgat ggactaattg
     3421 ccatcagtaa ttgggaaaaa catcaaaata tcgagggtat ggataaagta aggctaaaaa
     3481 atgctgaaag aaaccgtaaa tacagggaaa gaaagagaca ggaacgtctc aaattggaaa
     3541 atgacgttag cgtgacgtca cgtgacggta cagataaaga tatagaagaa gataaagata
     3601 tagataaaga agaaaagaaa ggtaagtatt ctgacgaaca cttacgcctt gctaaaaagt
     3661 tgcaaagtaa tttaactgaa gattttccaa aagaaatgaa caaagtagat atcgaaaaat
     3721 gggcagacac aatcaggttg atggaagaaa gagataaagc atctatagaa gcgattgagt
     3781 atgtgatcaa ttggctacct acaaatgaat tttggtttgg aaatattaga agtgctaaga
     3841 aattgagaga aaaatttgag aagctcaaat tcgaaatcaa agcagacaag aagaatcata
     3901 aaaagcaaag tcaaaaacta cagtacagca atcctagtga atatgacgac ttgccaattt
     3961 aaaaaggaga tgcatcacat ggaaagccta gcaaatgcta tggagaaact aataagaaga
     4021 gtattagtgc aaagcggaaa atgtccagaa tgtagcgaac ctttgtatag ttggcgagct
     4081 aaaaataagg atggttcaga acgttgtaaa ccaacatgca tgagttgtgg ttataaagcg
     4141 ttacgtgtga aagaggatat acagaccgaa cggatatata acgatagctt aaaagcacga
     4201 gcgttgagtt tttttcaaaa tggttcggta ttaacagata aaactttgtt taaatgcaaa
     4261 atggaaaatt atcacgtagt ggatcaagaa acaaaaatcg ctttagaaaa agcaaaaagc
     4321 tatacgaatg aggttctgct gaaccatcct gcacatttca ttctatcagg gaaatcagga
     4381 agcggaaaaa gccacttatc aatggccaca gcttgggaaa tacttgagcg ctcaaattat
     4441 gacaagaaaa tactttttat aagctatcaa gaattattag agcaaataaa gttttcttat
     4501 aacaatactg aactgagaaa agaaattgaa ggatcgctta tagccgatat taaaacaact
     4561 gatttggtgg tttttgacga tattggagct gaattaggta gtggggtatc aaatagtagg
     4621 cagtttacaa acaacacgtt aaacacgctc ttagaagcca gacagaacaa ggcaacgatc
     4681 atcacaacaa acttatctgg tcctgaacta agagaagcct acggtgaaag aattgtttct
     4741 aggatattta agaattcaga aggttatgcg ctgaaattcc aacaaacagc agacaagcgc
     4801 ataaaaccag tgaaaggtag tatcgcatga ataaataccg taataaaaaa actgttcatc
     4861 gaggtatcaa gtttgattct atcgcagaag cagagtatta cgatctagcc ttgtggcaag
     4921 ctgaagcaaa tggctggaaa gtaaaacttc aggaaagatt tgagctgatg ccgaaatttg
     4981 aactagacgg aaagaagtat cgcaagatcg agtatattcc cgacttcaca ttttataaaa
     5041 acggcaaact tgtcaaagtc gtagatgtca aaggaatgca gacaaaagac tttaagatca
     5101 aggcaaagtt gttctgtcat caatatcaag cgccgttgat attagctaaa aaatatcgga
     5161 atacgttcaa ggaagagcgt ttttaacgag gtggtccatc atgacaacag aagaagtgat
     5221 tcaaatgcgt attcgaaaca ttcagcgtga aattgacgat ctagaacgaa caaaggcagt
     5281 gatggtcaat gaaacggcga gaaaggcaat cgatttgcac atagagaact taagaaggga
     5341 aattcgtaga ttggaggaat gagcgtggat aagaaagcga caatgaaacg aattgctgaa
     5401 ttaaccaagt cagaatcttg gcaggaagac aaagaaatag ttgcagaagt ccaaaagctc
     5461 ggtaaatcaa tgtggactga aaagcctaaa cggaaaacgc cgagaaaaat tgcaatctgg
     5521 catggtgatc gaattctagt aacaggtaca gctgaacagt tatctgaaat tactggatta
     5581 agcaaaaaca ttatctggga tagagctagg agcttatgga ttgattcaaa aggacgacag
     5641 tttaggtatg tggaggagag ataatggatc tcattacaca atacagtgac atcatcctca
     5701 agaaaatcat gatgaagatt cagaaagaca aaaaatcaaa agaacgagct gaattagtta
     5761 agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg aaagcagcag
     5821 caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg
     5881 atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt gtagagaagt
     5941 ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa actcgttcaa
     6001 ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga atcggaccat
     6061 ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga tgatgtactg
     6121 tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga aatagtgaaa
     6181 aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg aagtttagag
     6241 cgtgggatag agaaaaagac agaatgaagg cagtagcttt ggttgaattt gctgaggcgt
     6301 ataaatcagg agaactcatt tcacgtacaa aataaaaaaa gtcggattcc tccgactatg
     6361 aataatattt ccgacataag tattatatca taattggggg aatcagagga tggtactttt
     6421 cgatgtaaag aaatatgaga caccaagcgc gaaagatgtt gatatggaac gcacaaaaca
     6481 taatgtcgct gtgtttcttt cagcatatct atcagctaga tgtagagtag ggcaacctcg
     6541 agaaccaaaa gtgacagcat cttattcctt ggttccacct tctacagctg atcatatatt
     6601 tgaagcagaa agaatgctga tcgataaaga agaagcacag gaagaatttg agtatctgca
     6661 taaattgttt gttcgaggct attcagcgat acagcatccg cataagcctg atgtgactga
     6721 aaggcgcaag aagatattct atgatcgtta tatcaatggt ctgcccattt atgtaactgc
     6781 tcaaaggaat aatactagcg aagaatcggt taaagtagaa tcaaacagaa ttatcatcca
     6841 atttgcttca tcgttagaac tggttgcttt caagtagcca gtttttacac tttttatacc
     6901 cttttattac caatttggtt tccattttat acctttttta taccaatcac ttacctattc
     6961 aacgttgtat tatgatagtg tcgaaagatt aggaaacagg atcgacaaaa taaaatgtaa
     7021 gggaggaaat ctccctcatc gtttaattaa gcttcgatag gcagcagcgg aaatattaag
     7081 aataaggatg tgaatttcaa ctccttctaa attgttctta ttatctatca tccgttgctg
     7141 tctattaatt tatgtattgg agggtatata aaatgaagat cgcttccgaa aaaattgatc
     7201 aactaacaat tttagtaaac caaagaatcg aagatattat caaagaattc gaaggtgaaa
     7261 agattaagaa cataaagctt actttaatgg atcataagtt cgaattaaat tcgacaaaaa
     7321 ggaagatcgt tgagcttata gaagaagaaa tagctctcca gatcaatgag caacaattag
     7381 ctaattaatt tatgtattgg aggggaaatg aatggaagtc gaacaaataa agtatcaagg
     7441 tatggatcgg gaacggttga ttgatattat tgagcaacaa cgtttagaat tgatccaaaa
     7501 agaagctgta tgtaaagagt acaaaaaaca tttggaacaa gtaattgagt atcattcagt
     7561 agaaaagtac agatcagtgg tgcaaaaaaa tagagcaacg agtcctgatt ctcgtcaaga
     7621 tgtatcgatt agtatcaaga cgccagtatt taattctgtt aaatcagaaa agctagaaag
     7681 tatctcaact gctttggtag aaaaagcagc tgaaatgaac tccctagcaa atgaagttag
     7741 tcgtcttatt tagtcatatc gataacttga gagtctttgg tgattccgtt ttttatagaa
     7801 ttaatggttt gttcagcgga atatttagtc aagtatgttt cgctagttgc tactacttca
     7861 ttgttattgg acttaataac gaagtaatat tggccattag ttgcttctct tataacaaag
     7921 tacaaatttt tcaccaccta taatttattt cagcggacca ctcgctgata attaaaatta
     7981 taagctatgt ataaaatttc acaatatcaa tttgtcgctg tggcggaaag ggtagacgca
     8041 ataaaggatg aatgggaaaa gtcaatgagt aattatctga aatttacaag tcccctgttt
     8101 agttatctaa gattatttga taatattatt tctgaggtga tagaaaatgg tgggcgtagt
     8161 taaatttctt gggtttacat ttgatggata tggtacgtta ggcgaatggg taggtattat
     8221 tttgatagtg attacaattc aacaaacatc aaagtactac tggtattcta atagttctaa
     8281 aattgtatca agaataactg aaggaataac caaaaaagat attactgtcg tcggtaaata
     8341 tgggtttact ataactttat taaatgaagg gaaatctgaa accgcaataa agttttgcgg
     8401 gattataggt aaatataaca ttttccaacg ttttatcaga aagcattggt acaaatatat
     8461 ttacaaatac cctgtagtta aaaagcttat tgaaaaatat gtggaccacg aaatatctta
     8521 tctcaatcca acagacctta ttaatcaaga attccagaat attggatact tagaaaaagg
     8581 tctaaaaata ccaattgtaa atgaaagttt agttactgca atggtagcta acataaagaa
     8641 cagcaagaaa ttagaaaaga gatataaaaa atgtaaaaaa ttgaaatttt cctttttatt
     8701 cttagaatat aatggcaaag aatattatca agatatttat gttttgcgag gctcgaactt
     8761 atataaagac attaaaaata agatttcagt atgagatttt ttaaaattcg tagatcactc
     8821 tttgagtggt ctttttattt tgcgtaaagg aggcacgacg atgtatagac cacaatactt
     8881 agaacagaag catgaagtaa tcactgtgca aaacggtaac ggtgagatag tacgaaagta
     8941 tagaagacca ataaagagcg atacatataa acggaaggaa agcaatgaag ttattccatt
     9001 gtatggcaaa agaatagcta agtattaaat aagattgcga aaggagatgg gacatgaccg
     9061 aggaattcta tagatggtta ttacagttga taagagaaga tcgtttggtt aagttctatc
     9121 agtctcctaa atggcgcagg cttagagaga aagcgatgaa acgagatcac tatgaatgcc
     9181 aagagtgtag aagactaggt aagtatcata gagtagagaa cgttcatcat ataaaggaag
     9241 tcaaggatag acctgactta gctttagatt tagataatct tatttgttta tgtgttgaac
     9301 atcataatga agttcatggc agatatctta cagcgttaga taaacaagag aagaagatag
     9361 aaagcttcgc taacttcgat gcaagtgaaa ggtggtaagt gcatgatcat caatgacaat
     9421 ggcagagagt atgatacaga aaagattgaa gagtattcat cttatactca gggattaatt
     9481 aaacgtttga tatacgttcg ctatgtaggt attagggatc tgttatcaga taactgttgt
     9541 agtaaataca aagtgaatca agtaagagaa gcgttgaata aagataataa cgtcgaaaga
     9601 ataaaaaatg tttttggata tagtattgaa gagattaatt attacattga cttcgctgaa
     9661 gctttcattc cgatggtgag ataacccccc cttaaaataa atcgcaaatt ttttgggggt
     9721 gatgaaacgg agggggctgt caggaaaaga gattttttcg aactttatca tgaaaggagg
     9781 gctaaaatgt ttaaaaacga attgtctcaa aatcgctaca gagaaaaatt acgccgctct
     9841 ttaataagcc aattggaaag tcagaaaaca aatattgagc cattcttaga taatgttgat
     9901 cgttatatca gtttatggga aacggcgata tcactggaag aagatatatc cgagaacggc
     9961 attagactgg agaatggtaa aaagaatgaa tcagtagcgt tgcttgtttc tgtcaacaaa
    10021 caaatgggat tgatgttgga taaacttgcc attactcctg aattggtagg tgaagcaaat
    10081 gaatcaattc ctgagttata agcatattga aaattggttc aaagctatag aagaaggcac
    10141 tatcaaggta tgcaaagagc aattattgct aaaaaattat ctagaagaaa gagtctttac
    10201 tagagaagat atttacttcg ataagcagat ggtagaggat tcaatcaata taccagcaca
    10261 atactttcca ttcgaattaa ttccgtggga aaaatttcta caatgtttta tttatggtgt
    10321 tcgatggaaa aaagataaaa cactagtgtt caatagatat ctttcattaa tgggacgtgg
    10381 taatggtaaa actggttttg cttcttggaa caacttcttt ctactaaccg ctaaacacgg
    10441 tattaaaaat tatgatattg atatctatgc caataatgaa agccaagcaa agactagttt
    10501 tgatgatgta tttaaagtaa ttaaagatca tcctgattta gataaaaaag tatttaaagc
    10561 tacgaaggaa gttattcaaa atatcgctac aaacagcaaa cttcgttata acacggcaaa
    10621 tgctagaaca aaagatggga agcgaccagg tgcaaaccgc tttgatgaaa ttcacgaaaa
    10681 tgaagattat tcaatgataa atgtggctac ttctggtggt ggtaaaattc gagattatag
    10741 agaattttat gatacaacta atggtcatgt tcgtggtggt ccgcttgatg acattataga
    10801 agaatcaaaa atgattcttt ctggagaact tggaattgac aaggatggag cagaattttc
    10861 tagtttgttt ccatttattt gtcgcttgga taacgataat gaagttgatg atcccgacat
    10921 gtgggaaaaa gcttgtccaa ctattaatta caatgcagat ttaaaacgga aaatgtttca
    10981 agaatactct caaatgcaac gtaatgctgg tttaagactt acgttcatga ccaaacgaat
    11041 gaacagacct atggaagata cacgatttgc tgttgcttca tatgatgatg ttctgcatac
    11101 gaaagaaaaa gaatttcctg aaaaaatgga tgaagtgata ggaacagtcg attttgctga
    11161 tagacgagat tttgccagcg ttgggttgct aggaaaatac gataaagatg tttattttac
    11221 acaacatact tttatccacg aatcagccct tcgattacaa aacatcaaac gagaggttat
    11281 agatatttct atagatcaag gaaaatcaca gatcgttcat ggaaaaaata tagaagctga
    11341 ttatattgta ggttggtttc ttgaaatgag taataaatat tatattaaaa aaatcgctat
    11401 ggatatgtac cgtgcaaaaa tattgaagcc cgctttagaa gaagcaggtt ttactgtgga
    11461 aattgttcga agcggatctg ttacacatgg tatgttaaaa gatctggttg atgacctttt
    11521 tattaatcaa cgtttatttt ttggtgacga tgcgattatg cgttggtatt gcatgaatgt
    11581 atatgaagag catatttcta atggaaatat acgctatgaa aaaatagaac ctgaaactag
    11641 aaaaacggat ggcttttttt cattccttca tggtttgaat tttttagatg atatttatga
    11701 ttctgctcct gtaacagtca caaatagctc agtagaaaat acaggaactg gatttactcc
    11761 tctagtattc taacttgaaa ggaggtgaga aagtggggat ttttcaaaag gcggtaggat
    11821 acttcacaaa aaaagcaacg gttcctttag aagaatactt ttgtaaattg caagttgatt
    11881 ttgtgtatcg aaaatttgcg attgaaactt gtattgattt gattgcaaat gcgatgagta
    11941 aagcggaatt caagtcatat gaagatggaa aaaataaaaa gaatgatctt tactataggc
    12001 tgaatgtagc tcctaataag aaaaataatg caacagaatt tagaaaaaaa ctgatcagga
    12061 gattaatatt ctacaatgaa gtattgatcg tttctccgtc taataattct agcgaaatat
    12121 ttattgcgga tagttgggat gtcacagaat atgcattgaa agatgatgtg ttttctcaag
    12181 tgcaaattaa caacatagtc cttgatagag aatttctaga aagtgatgtt atctatataa
    12241 aatacgcaga tcaacaaatt aggcaactag tcgatgcgta ttatcaagcg tatgggaaac
    12301 tcatttctag tgccatgaat gtttacaagc gctctaacgc tcgtagatac gtactgaaag
    12361 ggaatttatt ccgaccgcaa gacaatacaa cacaagatca aatcaataaa atgatgacat
    12421 cacaatttaa ggcttttatg gaagctgata atgcaggtgc ggtatttcaa ttacaaaatg
    12481 agtacacatt agaagatttc agcggaaact tccaaagcaa ttcaagagat ataaaaaact
    12541 taatagacga catctttgag atgacagcag cagcgtttca cgttccgaaa aacctactaa
    12601 agggagacat gagtgggtta tcggatcaag tggacgcttt tttaatgttc gaaatcatac
    12661 cgattgctga acttattcag gatgcgttta acgctagtct ctatgaagta gaagaatact
    12721 tgtcagggaa ttttgtacgt gtggatacaa ctatgatcaa gattactagc ttcaaagatt
    12781 tggttgacgc tattgatgta ggcattagaa atggagtatt tacaatcaac gaaggaagag
    12841 agcgcgttgg aaatgatcgc tctgataagg cgatggcaga tgaaatattt ataactaaaa
    12901 acaatcaaca agtatcgaaa ggaggtgagg cgaatgacga caatgaaaac atttctagca
    12961 gtaaagaatg aaggcgcagt accgcaaatt tttattcagg gatttattgg ttctagttgg
    13021 ttctttgaag ggaatactga caagggaatc aaaaatattt tggatagtct aggtgatcaa
    13081 gaagaaattg aagtagtaat taattcaaac ggtggagacg tatttcaagg gattgctatt
    13141 gggaacttac ttaagtcaaa taaagcaaaa gttaacgttg tgattaacgg cttagccgct
    13201 agtgctgctt caattatcgc aatggctggc gatactataa aaatctacaa caatgcacaa
    13261 ttgatgattc accgcgcttc cacatacgga gaaggaaatg tcgatgactt ccgtacgatt
    13321 gctgaccaac tggaatcaat tgataaatcg gtaaaggctt catataaaac acgattcaat
    13381 ggcacagatg aagcattgca agaacttctt gaaaaagaat cgtttatgga tgcagaaaca
    13441 gctttgagtt atggattggt cgatgaaatt atcgatgcag aaaatagctc aggtactgaa
    13501 gctaaaaaag aacaaagcgt tgaagaaatt ttgaatgacg ttaaagaaaa aagagcagaa
    13561 aaaattgctg catttacagc agcattaaat aaaacatttg gacaaggaga tgtaaaataa
    13621 tgacagttaa aaatttaaaa ggtgtaacag ctgcaagcga ccaattgatg aaagctttta
    13681 aagatggtaa cgaagaatct tttagcgcag ctatggtaag cttatctaag gaaattcagg
    13741 ataaaatttt agaagaagca acagcaaaaa atcaagatca attagtatta atgaaccgtg
    13801 gtcagcgtgt attaactaca caagaaacaa aattctataa cgaagtagtg aaaaacgaag
    13861 gttttgcagg ggtcgaagaa ttagtgccag ctactgtatt tgaacgtgta tttgaagatt
    13921 tagaacaatc tcatccacta ttgcaaaaaa ttacttttgt taacacaact ggtgtaacag
    13981 aatggattgt gtcacgtgga gtcaatccag catggtgggg taaactttgc gaagctgtta
    14041 aaaaagtttt agataatggc tttgatgtaa ttaacatgaa gcagttcaag ctatcaggtt
    14101 atattcctgt atgtaaggca atgcttgatt taggtccagt atggttagat cgttatgtcc
    14161 gtactgtttt agtagaatca ttgagaattg cattagaaca agcaattgtt gatggtactg
    14221 gtaaagatat gccagtcgga atgatgcgtg acatgagcaa acaaactagc ggagaatatg
    14281 ctgaaaaaaa agcagaacct attacagctt tagatgctgt aactatgggc ggtttgatgg
    14341 cgcgactatc aaaattcaat atcgaaggtg tgaatgatcc gatttatcgt aatgtgaatc
    14401 cttctgatgt ggtcctaatt gtgaatccaa ctgattactg gtctaaagta ttcccagcta
    14461 agactgtact aactgctaat ggagaatacg tacaagtatt gccagtacca gtttcagatt
    14521 tgcagtcaac ggctgtgcca gaaggaaaag cagttattgg ggtagcctca gattacttca
    14581 tgggtgtagg atctacacta aaaattgaag cttcagatga ataccatttt gttgaagacg
    14641 aacgcattta tctagctaaa caatatgcaa acggtcaacc taaacgtaac gatagtttca
    14701 ttgtattaga tattagcgct ttgggaacta ctactacaac tacaaaacca acaaccacaa
    14761 caactacaac acaagcgtag gtgatcagaa tgaagtatat tctttgtcag ccggcaatca
    14821 atcggtttaa atgggagctt gaagtttgtt taactaatct gaagaaacta ggaatcaaag
    14881 atatcgtatt gcttttcagc agacacgatg atcagattcc tatttttttt gagaaggaat
    14941 atggtgttga agttcatgtg tacgatgatc tgcgggacga caaagagtat attccttcga
    15001 ttaaaccata tttatggtgg aaatatttag aagaagatca ttcgcgtgag gacgaccgat
    15061 atttctatat cgattcggat gtcattttca ataaaagaat taatttgcgc aaattgcctt
    15121 ctaaagatga tgtttggtat tgtagcgact gctgtagtta tctaagtctt gattatatta
    15181 gaagctgtga aaacggagaa aatattctaa aagatatggc aaacattgta aatgttacag
    15241 tagaatcttt ggaaactata aacactaatt caggaggcgc acagtgggtt attaaccgcc
    15301 ctaaagcgaa ttattggaaa aaggtttatc tggattctaa tcggctatat cgctacctta
    15361 gagggcaaaa aacaaatata caaatctgga cagccgagat gtgggcacag ctttggaaca
    15421 tgatgtattt caatattggt cctaaagttc acgaggaatt agacttttgt tttgctactg
    15481 atccaataga aaaagttaaa gaagtaaaaa tcttacacaa tgctggagta acaacaaacg
    15541 atgaagattt atttttcaaa gggagatacg tgacttctac gccttttgat gaagatttat
    15601 catttgtaaa caagaaaaaa tgctcttacg catatgttaa agcaattaag gcggtggtta
    15661 gatgacgcct gaacaagtga ctgaagaatt gctaatagct gtgaaggata atatttacgt
    15721 tacctggaac gaagaagatg agtcaattaa aaagatgata gctaaaaatg ctgtttatct
    15781 tcaaagtaaa gtgagtacaa cactttcttt ttctcctgaa agcttagaat acggattgct
    15841 aatcgaaaga tgtagatacg actggaatcg tgctttagat gagtttgaac aaaatttcgc
    15901 tagtgagtta ttaggtttca ttcaacatta tgcgctacaa gaatatattg caggtgatgt
    15961 gaatggcgaa taatcgtaga ctcgaagaaa cattcaacga tggttggtta aagattttga
    16021 cgcaaactac caaaagaaat gaactaggaa aaaagattgg tgtagaagat acagaaatca
    16081 cttctttaaa atttagaaat ctttccatga gagatagtga tataacagct atggatgcga
    16141 tgggatcgaa attaactaag aaagtaaaga ctccatttca tccaatcgcc aagaaattta
    16201 ataaagatca atattttatc gtaatcgata gtatgcgtta caacgttatc tatgccgatt
    16261 acgataattt ttatatctat ttttatcttg aaagtgtggg tgaatatggt gattgataat
    16321 tctaaagaaa aagaacgttt aaataagcaa atttctgcta tcaaaacttc cttagaagag
    16381 cattttggcc tcaaactctt tcaagactcc gtaggcgagg atgagctacc tgatgatttt
    16441 aattacttca ttctcgaaac aggagaaata gaaatgatca ctgagccaaa atatagcgtg
    16501 ggtcaaaatc tatatctaac tttctattca gaaaatagag aagatttaac aggagattca
    16561 ctagatatta tttcattgat tcaaaatcgt tcgattcgtt ttcagagaat ggatcccaat
    16621 catttaaaac tagagaacca agatcgctat atcgatcaat tggtatttac gtttagacga
    16681 ttattgaaga gtgattgtca tggctaaaaa tagttgggag ctaaaaataa atggacatga
    16741 tgaacttctt gtgcggatgg aacgctattc aagcgagagc gaacgactga ttaacgaagc
    16801 attgaaatca aaaggttcgg ctattgcagt ggataggatt acagaaaaaa ttcctgtttc
    16861 tgaagcagat ttaagaagag ggcaccaaca cgcaaaaaat agtcgtccac ttaagactca
    16921 atacattaat ttgggtttca tcattagacc tacaagaaaa tttgagtatt taaaatatcc
    16981 tgatttgggg ataggtactt ctaaaagaaa tcagccggac gaatttatga gaagaggatt
    17041 aggtcttgca cttgatccaa ttacagaact tctgattcgt caattcgata aattaaataa
    17101 ataggaggaa caacaatggc taaaacaaca acagtaacaa cttttgacaa tataagtatt
    17161 aaaagaattt cttttaattt taagaatgca acaaatgcaa tttcaactga ttgtaatgga
    17221 caattagatg gcgaaacaga aatgcagaga attgtaaaaa aatgcggttc aacagaagta
    17281 aaatcgaaat ctaaaccaat caatatgacg gtaacaatta ctgcacatgt accgatggaa
    17341 gtttatcgac gcttcaatgg gttgaaacaa gatgaacgta ttaaatcggg aatttactct
    17401 tatggtcctg attccgtagg ggaagatttc tctcttgctg cagagatcgt ggatgatttc
    17461 gaagaaaaaa ataagctgat tggtatgtta gcatgcactt cgaatacagg attaacattc
    17521 tctattgaaa atggtgcgga tgaagtagct gcgttagaac tagaaacaaa agttatgcaa
    17581 gatgaatttg gtaaatttta tcatgaagca attgttgcag aacttgaaga agacttaaca
    17641 gatcaatgga tgacgaatct atctgctgat gtgattaaaa aaacttcagt tgtgacaact
    17701 acggccactc aatcacagta aaaaaaacgg aggtagcgaa atgaacgaag attactcaaa
    17761 aattgaacta aacgatggaa caattttgaa tttagaacct aaactgaata tcaagaaatt
    17821 attgatgatc aatagagatt ttaacacaga cgagtttgca aaaatgactg tgggaaaagg
    17881 atccatggat atttctgtta ttcaaggtgc aaaggctgtg tatattgctt accgccaagc
    17941 gaacatgact gattatattt cattcgatga atttatcgat aaatgggatt ttgatatggc
    18001 tactgccagc tatatttatc aattgatgat gttcaaacaa gcacgcgatg cttatcaaaa
    18061 agaatttgaa aaagcaaata aggaaaaaaa gcttcaaaag taaaaatgcc aaagctctta
    18121 gttgaaacgt gggtcgatgt ctattcgatg ttgaccgacg ttttttctat gccttcagat
    18181 ttggttttaa gcgatatctg tttagatgac attttgcaaa tggcttacaa caagagtgct
    18241 tatgaaggat ggaaaaacta tgcaataaac caatcccaga aaaactaaag aaaggaggta
    18301 aaaaatggct aaaaagagaa cagaagcaga agtaacattc atagctaacg atgacggatt
    18361 gaaatctacg ttaaaagaaa tcagtgctga attaactaaa aatagagcag aattaaaact
    18421 agaacaagct caattacaac agactggttc tgaatcagac aagttaggaa gtaaattatc
    18481 ttctttagaa aagcagtatg aattacaaag tcaaaaagtt gaagtaacta gccaacgttt
    18541 agccaatgcc aaaaaatatt atggagaaaa ttccaccgaa gttcagaaac ttgagagaga
    18601 actgattaac caacaaacag cgcaacaacg tttgtcaaac gaaattgata aaacgagtaa
    18661 tgcactagct caagcaaaag gcgaaataca gacgtacgag tctacaatgc aacagttgga
    18721 tagtgaacaa aaaaatgttc aagctagtgc ttctctgatt gaatccgaat acaaaaaatg
    18781 gcaagcaact gctggtcaat cagcttctga atccgagaaa ttagcgaaag cccaagaata
    18841 tgtttctcaa caatctgaaa atgcagagaa aacgatagat atcctgagac gacagttaga
    18901 agctacacag tctgagtttg gcgctacatc cacagaagca atgcagatgg aggcgaagct
    18961 taatgatgct gaacgtgaat ttgaagagtt aggacaagct gctaaaaatg tagatacaac
    19021 taacttggac gatatcggaa gcaaaataga tatgaataat ctaatggaag cttctgacgt
    19081 tttaagcgac attggcgata agcttacaga attagggaaa caagcagtgg actctgctaa
    19141 tagtgtaggt agttcccaga gtaaaataca agctaatttt ggtttgacta aacaagaggc
    19201 tgaagaatta acgaatgtag ccagagacat ttattataaa ggttttggag aatcgttaga
    19261 tcagtccaca gatgcattga ttttggtaaa gcgtaattta ggcgatttaa ataatcaaga
    19321 tttacaaaat atcacggaac aagctatggt cctagaaaac accatgggcg ctgatatgga
    19381 tgaaacgtta cgtggtgtaa atggcttaat ggtcaatttc ggcttgagcg ctcaagatgc
    19441 aatggattta atggtttcgg gtactcaaaa cggtttagat aaaacgcacg aattaggcga
    19501 caatatggca gaatatagcc aattatggag tcaaatggga tattcagctg atgaaacgtt
    19561 cggaatgctt caaaatggtt tagatgcggg tgcttataac cttgataaag tcaatgactt
    19621 agttaaggaa atgggaatat cgttaacaga tggtcgattt gagcaaaaca tggatatgtt
    19681 tagtgaaagt actagaaaag cttttgaaga gtggaaaaat ggcggaggaa cacaaaaaga
    19741 cgttattaat tccatgattc aagattttag caatatggat ggtcaatacg accaattaaa
    19801 taaagcttct acaatttggt ctgcacttgg cgaagataac gcgatgaaag ttgtccaatc
    19861 tttgactgat gttaaccata catttgatga tgttagtgga tctgcacaaa aaatgaatga
    19921 agattctact actccgttac aagaattaaa cggaaaaata gctgaattaa aggattcatt
    19981 agctcctata ggcaacacaa tcatagatgc actcgaacca gtaattgatt ttctaggaaa
    20041 gatggctgat gcgtttaata atcttccaca accagtacag gattatgccg tagcgattgg
    20101 cggattgact gctgcattta ctttattaat gccaataata gttggcttca tggctctagg
    20161 tggtcctact acattaataa taggagcagt tattactgtt attgctggag ttatagcaat
    20221 tataaaaaac tggggcgcaa ttactgactg gtttaaggga atatggagta aattcactga
    20281 ttggttgggt ggtacttggg aaagtataaa agaaggtgcc tcatcagttt gggatggagt
    20341 taaagaaacc tggtctggat ttgtagattg ggttcaagat atttggcaag gagtttctga
    20401 ttggtttgga gagctatgga gcggattagt tgaaggagct tccaacatct ggcaaggagt
    20461 ccaagagact tggcaagcat tcgttgattg ggtttcaaat atttggaacg gagtcaaaga
    20521 agtatggtcg attatttggg cagacattgt aggaattgtt caaataccat ggaccttaat
    20581 aacgtcattg attcaagccg gtattaatat tatcgtgggt atttttgatg tagctggaca
    20641 gttattaggc gcagcttggc aagctgtttg gacacctatt tctgatttcc ttaaaaacac
    20701 ttgggatact atgacacaat gggtaagcat cgcttggaat ggaattgtaa ctacattcca
    20761 tactatattt gatccagtag tggcatggtg gaatggtata tggacagcta ttagtactac
    20821 ggcttcaaat atttggaatt caattagtgc aacagcttct agtatttgga acagtatcaa
    20881 gaatacaatc actagcttgg tacaagcagc tgctacagta attcaaaata tttggtcaac
    20941 tgtatctagt tggttaggtg gaatttggaa ttcaatcagc tctacagcat caaatatctg
    21001 gaatagcgtg actagtagta taagcaatgc tataaacgca gctaaaagtg ccattcaaag
    21061 tgtttggaat agtatatctt cgtggatcag cggaatttgg aacggtatca aaaacactgc
    21121 tttgaatctt tggaatggaa ttacaagcac tattagctct aaagtaaacg atggaaaaaa
    21181 tgcaatttca agcggttggt ccaatctaac aggtattgtt tccgacatat tcaataatgt
    21241 taaaagtaca attgctaaca tttgggaagg tatcaaaaag actgttagcg ctccaattga
    21301 ttggatcaga gataaaatca gtagtatctt tgataatttg aatatttcta taccacatat
    21361 tccgttacca cattttaaat tgagcggaga attcaatcca ttgaagggga aaatcccaac
    21421 gttgggtgtt gattggtatg cgaaaggtag tgtgtttaat tctccgaata ttatcggtgt
    21481 cggcgaagca ggacctgaag cagttttacc tttgaaaaga tctgtgctgc aagaaattgg
    21541 tgatcgtatc ttgagtagca catcagtttc atctagggca caaacgattc aacctgtgaa
    21601 taactacgaa ttcaatttca caattgatgg taacgcagat gaggttacta tgaagcaaac
    21661 aactcaacaa atcattgata gcattacaaa agttcaaaat gataatgctt cggcatggcg
    21721 ttaaacagga gagtatttct cctgtttttt tagtattaaa aaggatgtga aaaaatgact
    21781 gattgtatac attctataat cgatggattt cctgattatt tgcataaatt ggctttagct
    21841 gaaagaccaa ccataccttc tccaaaaaga cagagagttg aaacttctgt tttaggaagg
    21901 ttaggtggct tagtacaaga ttactcgttt gaagacatgt cgtttacatt gcactataac
    21961 tatttagagg atgtggaaga ccatcaagcg ttcaagcaat cgttttatat catgcgtcat
    22021 tggttaaatt atgcaaagaa attagaattc tctgatgatc ccaacgtcta ttatgttatc
    22081 cagactatcg atattgggga tgcagaaaac gatattgttg aatggggaga gttcgatgta
    22141 aatattactg cgaaaccatt cgcaagagtt caagaagatg tacctataac cgtagataaa
    22201 ccacagtcat ttaacttgct gaataatagt ttagaagaaa gttttccaaa gattatcatc
    22261 actccttcag ctacttcatg ccagttcatc ttaaatgatt atgtgtttag ttttgaaggc
    22321 ttagtagtag gaactgacgt agtcattgat agtgatttga tgctttgcta cgaagagcaa
    22381 tcggacggag atattttaga tcggtccaac aaaatgaaga ccatgcaata tccgacattg
    22441 caagtggata ttaattattt taattgtact ggtttgagca aaatacaaat ttatcgtaat
    22501 gggttaaggt aggtgaaata gatgatcgat aatttaataa ctatttacga taaaaacgac
    22561 gcgaataatt tagctgaaca tttatatgat acgcaaggtt taggcgcttt gtcagactgg
    22621 ttaacagcta ctgttagcaa taaattaaac ggagccgaga tatttcaggg tacttatcca
    22681 ataagcggaa ctaatgcaga tttgattgta gaaggacgta ttattcagtg ttatgtagat
    22741 gaaaatcgag caaaacagcg tctacggatt tattatgcaa agacttctgt aataggcaat
    22801 acgatagaag taaaagctga acctattttc aatgatataa gaaaatcggt gttgaataaa
    22861 tatgacagtg gaacagaaaa gatcactgct agtcaggcat ggcaaaacgc aaaaacttta
    22921 gcgaaaccag ttattccttc acagttttct ttctcgtcat tagtagatac gcttgctaat
    22981 gtgaagatag aaaaggcgaa ttttttagaa ttctttggtg gaaaagaggg atctattcta
    23041 gatcgatttc atggggaatt tctaaaagat aataacacat tacgtcatga aaaaaggcta
    23101 ggcacggatc ataaaatcaa agcgatttat actaaaaact taactggtct tgacttagag
    23161 attgatgctc aaagtgtttt agttggagtt tatccattca ttagcagctc ttcagaagga
    23221 gaagacgaga tcactctacc agaagaagtt attttcacgg attacgtgga tgattatcct
    23281 gctggatatg tttcttttgt tgattttaaa gacaaagcga ctgatgtagc cacattaagg
    23341 gaagctgcta aagactggtt gaaaacaaac atagataaac aaaaaccaca agtgagtggt
    23401 tcgattgaat tagtaccatt gaggcatcaa agaggctatg aaaaatttgt tgatctagaa
    23461 aaagtttcga tgggtgacgg agtagatgtg tatcatccac agttaaaagt gaatatgtca
    23521 gcgagaattg tggaatatac gtttaatgtt ctaaccaatt catacgataa attagttgta
    23581 ggaaacgtca aaacaaactt cttagaaaac acagagaata atgtcagcaa tttgattaat
    23641 gatgccattg atcaattgaa aaacggtggc gaaatcagtg atttactcaa tgatattgta
    23701 gatcatcaaa ctgatatgat tactggtcaa aatggtggtt atgttttatt agatcctaaa
    23761 gaagcgccta gtcgtatttt gattatggac acaccagata agaataccgc aaggaatgtt
    23821 ttacaaatca acaatgctgg tattggtttt tctaaaactg gcattaatgg aacatatgaa
    23881 acggcatgga cgttagatgg cggattcaat gcctcgttta ttacggctgg tgaaatagta
    23941 ggaattacta ttagaggtac tacattaatt agtgatggtg ctgattatag aacaagtatt
    24001 gctaatggca aaatgacttg gtactcaaaa aaagttaaca aagatattat ggagctagaa
    24061 gcacgtgatt atgtaagtgc tgatgccggt attgtatcat acaccatgaa aactggtggt
    24121 ggtttcatga ttagaaatcc acagggtaac ttggttttta gtacgtggga taatggtaat
    24181 aacagaccgt ttctatcttt tggtgcgccc aatttcaggt atagcaatgc tagttatgta
    24241 acttctggcg acggtagttc tttaagcatt aatggtagtg cgggtaactc atgggaattt
    24301 aaggtagctg gtaggactat gaaatttact agtgatggta tgctaacgtt accaggttgt
    24361 ttttttggtt catgggaaga tgggaaactt gctaggtttg aacaatcaac ggtacaagta
    24421 tataaagatt ttactgttag aggtactaaa aactcaactg taccaacaga acattatgga
    24481 caacgactat tgaacgctta tgaaactcca gaatattatt tcgctgatta tggggaagcc
    24541 gttacaggtg acgatggtaa agttcgtgtt gatattgacc ccatgtttgc tgaaacagta
    24601 aatctaagtc ggtatatgac acatgtgaca cctacagaac tagttttgtg tgctgttact
    24661 catgaagata ttgaccattt catcattgaa actagtaagc caaacgtatt agttagatgg
    24721 aatttagtgg cacaccgtct agggtatgaa gatattagat taaaagagga tacagcatat
    24781 gatagcacag tgcttgacca aaaacgtttt taaaacgaag acaaggaggt atataaatgg
    24841 ctagcagttt atataatttg gctttagatt tcagcaaaga attaaactac accaaagcta
    24901 ttatggctcg tcaaggtgat aaagggatta cggtgacggt taaaccgttt ctaaatggct
    24961 tgcagatgga tacgagtggc ggaacattta ctttaaaagg aacaacacca tctaaccgtt
    25021 acgtagataa tgttgcaact agtgtaacta gtgaagaagt cacgttttct cttgatggca
    25081 catttatgag tgaagcagga tattataaac actgctacgt agaatataga aaagacaatc
    25141 aaattttaac aacgcaagat atcatttttt tctcactagg agtgtctgac atttcgcaag
    25201 gccaagccga tgaatatgtt tcgcaattag aagagttgat tcgaaagtac aacgaaactt
    25261 ttgatgcttt tatggctgaa atcaaaggta gagtggatag cttaaatcaa cagattactg
    25321 atttaactgg tcaagctaaa acgctacaag acaagttaga tgctctgaaa gaagaaattt
    25381 ctaagttagg taacttacaa gtgatgtaca gtaacagcat cgacttcggg ggatatgatt
    25441 atagcggtaa gccgaattta atgtccaaac taaaatcgag cgattttaac gttggttacc
    25501 acgggtcact aactttggat aacgaaaagt tacattttac ttctgatggt acaggaagca
    25561 ttattatgtt tacgcgtatt aatacacctc agcttactag tgggaaaacc tatactctga
    25621 gtgcgaaagt tcgatttgat gaaggaacta caggagctat tgataaatta cgtttggtgt
    25681 atcgtacatc accaggagaa aagatattat tggaagcaaa tagtacaaat attacaacag
    25741 atgatgtagg gaaagaaata acaatcaaag gtacagctaa cgttaattat caaatcacaa
    25801 atttagatcg attttatatg agtattagct ttgttgacag ggataaaata aatggcggat
    25861 ttaagttgta cgacatcaaa atcgaagaag gctcaacagc cacaccgtac cagcctaatc
    25921 tacttgatgc accgtattat ttgagtaagg tggctttggg tgagaatcta attaaaccag
    25981 aatcacaaca accagttact aatagtaact atcttattaa cacctataac attaaaccaa
    26041 tggtaaaagg taagaagtat accatcacac ttgaaggaac taagccagca acacaggttt
    26101 ttagaccatt tttcacacga gcaacaggag atgcatggga ggttggtgac ttacaaccag
    26161 tagaaggctt aactaatgtg tggtctaaaa catttacagc agcagatgac tcacacccta
    26221 ctaccccaca agtgcagatt tatcaagtac caagcacaag tgtaggacaa tgtacaatta
    26281 agtggttaaa actagaggaa ggcaatactc gaaccccgaa tattagtgag tataaatatc
    26341 gtggtactgg tatgcgtgat tcaaacaatc caaaagatta tgtttgggat ctagcaccag
    26401 aatatgtcga agataatttg gccacagata ttaaaatttc tgaaattact ggtaaagcaa
    26461 acaattatac cgatgggaaa gtatcggaga ttaattcgca gttgactgct tcaattaatg
    26521 aagtagacac cacagctaag gatgctcaaa caaaagcgaa tgctaatgcg actgctatag
    26581 atgaattaga caataagatc gatgaacgca ttaatgatac agctactacc acattaacag
    26641 ttacaaacgg gaataccgga tcagcaaagc tttatcgtga aggaaaaaca gtttctatat
    26701 attttgtggc tttaaacgga aaacgcagtg gtggaaatga ttcaacgata ctaacaattc
    26761 cagaaggcta tcggccacca attagttttg agcaactggt tggctcgata gaccgttcta
    26821 ctttgaacag tgctcagtta tctattggtg cagatggagc cattaaatgg cgaagaaact
    26881 caagttatgg atcggattat acctttgcaa ttacttacac gatttagaaa agcgtgaatc
    26941 gatatgaagg cagcatatag accaattgaa ccttacggat tcgagcaaat cattgtgaat
    27001 gatgaagaac atttaccgga agaatgcaca gaagtcgaac caccgattcc aaattggaaa
    27061 ccgaaattca attactggga gggaaataaa tgaaaaacat ttggaaatat ggacgtactg
    27121 gcggagagta cgcaggaaaa gtattggacg acatgcttgt atccgttcct tacacagatc
    27181 agccaccgct tgaaggaatt cgtgctgatg gcgaaccgtt aacaatcgct gatcaaatgt
    27241 ttgatcctaa attgaatcaa tggattattt tagcgaacgc actagatcac aacgatttaa
    27301 acaatctcaa agcaatgtat gagtcgttag aaaatgagaa cggcgattta aaacagatca
    27361 atgccaaact catgctaagc gatgtagcaa ttaaacagga aaatactgca ttgaaagaaa
    27421 aagcggatag tttagcacaa atcaattcaa aaatgatgct tgcttcgtta caaaatagca
    27481 aagacatttc agaaattaaa gagcaactaa atccagcttc aaagggaggt gagtagtatg
    27541 tttagtttta gcgatgtgaa aatgatgtat gattggggct gtttcactaa cgaacaagta
    27601 atggttttcg ttccgttgtg cattactgaa gaaaaagcag ataaaatcat tagcaaagaa
    27661 gagagcgcat cttaattgat gtgcttttta ttttgattca aggagttgtc acatgattaa
    27721 tttaggggaa tggggagcga tagcaggatc aataaccgct atcgtttctt tgattttatt
    27781 agtaataaaa ccaattactg catctttctc gaagattact gagactcttt caaaagtaag
    27841 tcacaattta gatttgctga ctaaagattt agaatcgagc aaatcagatc gattgatgat
    27901 tcatgaagaa ctaaagaaac acgatgaaag attagataca catgcagaaa aattggtaga
    27961 acacacgcaa caaattaaaa ctttatttag ggagagaaga aaatgaataa taaaacgttc
    28021 gaagtactaa aatggttcgc actggtaatt attcccgcac tagctacttt cgtggggtta
    28081 gttggtaaag cgctcaattg gcagtacaca gatatctgtg ttgtcatcat tactggtttt
    28141 ggcgcgtttt tagggagtgt gttgggtgta tcaaatcgaa cctacaaaat gttctcggct
    28201 gaaagcgaag aaggaggaaa caaatgaaaa agaaaattac tattactgcg atgagcctgt
    28261 taacggctct ttttttatta cccattaata cgtttgctta tactattaat gacgagtata
    28321 atttagcgcc gaatcaagga gactccagat tagcaattcc taacaaaatt attttgcacg
    28381 aaactggaat agatgcacca gcaagaaacg tagccgccaa catgaaaaat aattataacg
    28441 gaagcaattc ttatactaca gatgttattg gtgacggtgg gattgtttac cgtgtgggtg
    28501 agcaaggata tgtttcgtgg ggagctggta acgctaatcc ttatgcgcct gtacagattg
    28561 aattacagcg cacatatgat aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat
    28621 atacaagaga tagtgcaaaa aaatatggaa ttccattgac tcttgatcaa ggaacttctt
    28681 tatttacaaa aggaatcatt tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca
    28741 cagatccata tggttactta tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt
    28801 tagctcatgg atttaccgat gaaaatccaa caacttctga aaacaagcct gtcattgatc
    28861 caacacgagc tggtgcagct aatcctacgc tgacagatgg aacaaatcac gcccacattg
    28921 atcagtttgg ggaaatcgaa aacgcaaact tgcatgtggc tggatggcac attgctaact
    28981 ataaatacga gtatattttc attatggact acaatactgg gaaagaatta gctcgagtaa
    29041 gagctgatgg aatttataga ccagatgtaa accaagctta taatacttct ggaaatgttg
    29101 gttatcatgt atctttcaat atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc
    29161 gggcaacgaa tgatccagag ggaaacacta aaggcggtgc gcaagatttc catgacaaac
    29221 gttggtattt aaatattcct aaacgataaa aatagctcct cgttgaggag cagtacataa
    29281 ctatattgac aactataaaa attattcgat aaaatagtga tgttatcgca tatcttcact
    29341 atcacccat
//
LOCUS       DAFGZE010000032        27928 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.032, whole genome shotgun sequence.
ACCESSION   DAFGZE010000032 DAFGZE010000000
VERSION     DAFGZE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.032"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(10..216)
                     /locus_tag="LTU54_001542"
     CDS             complement(10..216)
                     /locus_tag="LTU54_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL8296260.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(349..1308)
                     /locus_tag="LTU54_001543"
     CDS             complement(349..1308)
                     /locus_tag="LTU54_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8296261.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            1574..1918
                     /locus_tag="LTU54_001544"
     CDS             1574..1918
                     /locus_tag="LTU54_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBL8296262.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1919..2338
                     /locus_tag="LTU54_001545"
                     /pseudo
     CDS             1919..2338
                     /locus_tag="LTU54_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(2430..2780)
                     /locus_tag="LTU54_001546"
     CDS             complement(2430..2780)
                     /locus_tag="LTU54_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296263.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2746..4077)
                     /locus_tag="LTU54_001547"
     CDS             complement(2746..4077)
                     /locus_tag="LTU54_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HBL8296264.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            4333..4899
                     /locus_tag="LTU54_001548"
     CDS             4333..4899
                     /locus_tag="LTU54_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8296265.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            5044..5131
                     /locus_tag="LTU54_001549"
     tRNA            5044..5131
                     /locus_tag="LTU54_001549"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5076..5078,aa:Ser,seq:cga)
     gene            5465..6439
                     /gene="bsh"
                     /locus_tag="LTU54_001550"
     CDS             5465..6439
                     /gene="bsh"
                     /locus_tag="LTU54_001550"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL8296266.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            6764..8659
                     /gene="asnB"
                     /locus_tag="LTU54_001551"
     CDS             6764..8659
                     /gene="asnB"
                     /locus_tag="LTU54_001551"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBL8296267.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEKWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(8854..9300)
                     /locus_tag="LTU54_001552"
     CDS             complement(8854..9300)
                     /locus_tag="LTU54_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HBL8296268.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            9528..9677
                     /gene="rpmG"
                     /locus_tag="LTU54_001553"
     CDS             9528..9677
                     /gene="rpmG"
                     /locus_tag="LTU54_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBL8296269.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            9765..10283
                     /locus_tag="LTU54_001554"
     CDS             9765..10283
                     /locus_tag="LTU54_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL8296270.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10360..11316)
                     /locus_tag="LTU54_001555"
     CDS             complement(10360..11316)
                     /locus_tag="LTU54_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8296271.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            11482..12288
                     /locus_tag="LTU54_001556"
     CDS             11482..12288
                     /locus_tag="LTU54_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296272.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            12285..13274
                     /locus_tag="LTU54_001557"
     CDS             12285..13274
                     /locus_tag="LTU54_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL8296273.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            13271..14275
                     /locus_tag="LTU54_001558"
     CDS             13271..14275
                     /locus_tag="LTU54_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBL8296274.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            14407..14949
                     /locus_tag="LTU54_001559"
     CDS             14407..14949
                     /locus_tag="LTU54_001559"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBL8296275.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            14969..15667
                     /locus_tag="LTU54_001560"
     CDS             14969..15667
                     /locus_tag="LTU54_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL8296276.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            15688..15900
                     /locus_tag="LTU54_001561"
     CDS             15688..15900
                     /locus_tag="LTU54_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HBL8296277.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            15900..16862
                     /locus_tag="LTU54_001562"
     CDS             15900..16862
                     /locus_tag="LTU54_001562"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HBL8296278.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            16880..17275
                     /locus_tag="LTU54_001563"
     CDS             16880..17275
                     /locus_tag="LTU54_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBL8296279.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            17438..17803
                     /locus_tag="LTU54_001564"
     CDS             17438..17803
                     /locus_tag="LTU54_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HBL8296280.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            17800..19611
                     /locus_tag="LTU54_001565"
     CDS             17800..19611
                     /locus_tag="LTU54_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HBL8296281.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTNENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(19673..19840)
                     /locus_tag="LTU54_001566"
     CDS             complement(19673..19840)
                     /locus_tag="LTU54_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HBL8296282.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            20088..21077
                     /gene="galE"
                     /locus_tag="LTU54_001567"
     CDS             20088..21077
                     /gene="galE"
                     /locus_tag="LTU54_001567"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBL8296283.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            21089..22573
                     /gene="galT"
                     /locus_tag="LTU54_001568"
     CDS             21089..22573
                     /gene="galT"
                     /locus_tag="LTU54_001568"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HBL8296284.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            22597..23577
                     /locus_tag="LTU54_001569"
     CDS             22597..23577
                     /locus_tag="LTU54_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296285.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            23768..24490
                     /gene="tsaB"
                     /locus_tag="LTU54_001570"
     CDS             23768..24490
                     /gene="tsaB"
                     /locus_tag="LTU54_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBL8296286.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            24474..25016
                     /gene="rimI"
                     /locus_tag="LTU54_001571"
     CDS             24474..25016
                     /gene="rimI"
                     /locus_tag="LTU54_001571"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL8296287.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            25023..25487
                     /gene="rimI"
                     /locus_tag="LTU54_001572"
     CDS             25023..25487
                     /gene="rimI"
                     /locus_tag="LTU54_001572"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBL8296288.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            25484..26500
                     /gene="tsaD"
                     /locus_tag="LTU54_001573"
     CDS             25484..26500
                     /gene="tsaD"
                     /locus_tag="LTU54_001573"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBL8296289.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(27109..27810)
                     /locus_tag="LTU54_001574"
     CDS             complement(27109..27810)
                     /locus_tag="LTU54_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBL8296290.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
BASE COUNT         8986 a         4820 c         5754 g         8368 t
ORIGIN      
        1 tctatcctcc tatttatctg atgaaatttt cttatacgtt cgatttgata cacctaaaat
       61 actccctaga aatgctccaa aacctgtaat aatcatcacc gttatatctg tgtaccgcca
      121 gttaacacct tttccgacaa gaccgacaaa agtcgctaaa gcggggataa ctactatagc
      181 gatccatttc aaaatatcaa aggttctatt gttcatacta tccacctccc ttcattggtt
      241 gggctacttt ctttttcaat cgcttgtttt cttctatcaa tatttctctt tctgaaagca
      301 aggcatcaat ttgcccgtcc agttgttcaa ttttatcttt cttaattgtc aaattaagtt
      361 agacagatca tcaatactta cgtaactcaa aaatacttcc aaaggtgttc gataatccag
      421 tgattttcta ggaatattat ttcgtttaga tgcgacagat tgaataaaaa attcatctac
      481 tgaattgaaa tccatagatt tcaataaacc atctctacgt aacaatccgt tagaattctc
      541 gtttaggcct ctttgagacg gcgttcctgg atcagcgaaa taaatggcaa tatcattgac
      601 attactgatc tgtttccaat ttgaaaattc ctttccacaa tcaaaagtga tggatttgaa
      661 taggtttttc ggtacagatt caaaccattg atttaatttt ttttcaatat caatcgcttg
      721 tctaccacac ggtttcaatg tgatgataac ttttgataat cgttcaacta aggtaattac
      781 agcactttta tgtttcagac ctacgatagt gtcaccttca aggtgaccaa actcatttga
      841 gaattggcta taatcctttt cacgttcatg aatagagcgg cggaaagttt gttttccacg
      901 tctttcttga tgtccattcg gtttacgctt gcctttcatc ggtaagtcag aagaatcaaa
      961 tagccccttt ttgaacatac gataaatggt acgagcagaa caactaatag gaaacgctgc
     1021 acgaccaaca atcacatcgg gtgtccatcc ttgaacaacc tttctttgaa tatattctga
     1081 ttgttcctcg ggtaaaacaa gcggacgtct accacagttt gatttgtttt tcttatactg
     1141 ttgataatat tctaaggctg attttccttg cttgaagaac agatatactt tatggatcgt
     1201 ttgtcttgaa cgattcaagc aatgggcagt tttagcaaca gattgattca ttttgaaata
     1261 agactctatt attacaagtt catccgttgt aagatgggta taggtcattt gtactcactc
     1321 cttataattc tttggtcgga actattttga gtgtagcaca aatgactttt ttagttgtct
     1381 agcttaattt tacaatcggc gaataaaaaa agcgttagca actatgctta aaaagcatat
     1441 cgactgaata aactaagcat tggacaggct gaaagactac atttaaaatg aaatgcgaag
     1501 aaacaataga aaataaagtt gccttagaac catagttgaa tcacctaaca gtcgtgaagg
     1561 gagaataaga gaaatggcag aaaaacaaac cgcaggacga gatcaattag gagattttgc
     1621 accaaaattt gcagaattga atgatgatgt tttatttgga caagtatggt ccagagaaga
     1681 attatctgct catcagcgta gtatcatcac catatctgca ttaatttcag gaggaaattt
     1741 tgaacaattg ccagctcatc tgaagattgg caagcaaaat ggcattacta gtgaagaaat
     1801 agcagaaatc attacccatt tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt
     1861 tagtttagca aaagaaatct atttagaaga aaatagcgaa gataagaaag aggcgtaaat
     1921 gacgacaaaa cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga
     1981 agcttacgct caagtattta ccgggtaaag ctatctgaat atgttagtga atgatcccga
     2041 agtttctgtt ggtgtaggta atgtaacttt tgaaccaggt tgtcgcaatc actggcatgt
     2101 ccataaagac ggctatcaaa tcttacttgt gaccggagga gaaggctggt atcaggaaga
     2161 aggaaagcca gcaagacgat taatggctgg agatgtcgtc gtaacgcaca aagggattaa
     2221 acattggcat ggtgcgacaa aagacagttg gtttgaacat ttggcgatca ctgctggtaa
     2281 ttcagagttt ttagaacctg taacagatga agtgtatgaa ggattggaaa atcaataatg
     2341 tcaagcttga acagctcctg aaagtagatt ttaatcgaac tttcaggagc tgcttttttg
     2401 attcagtctt ttataaatag aaaatagggt taataatagg aatgagaagc acgtcgaaag
     2461 atcttaatat tgataatcgg ctgggcatac atgatttcca cgtcatcaat caaactacct
     2521 aacagatgat aatcttcccg atcatgtcgg ggattaccta atagttcttg atagtagatt
     2581 tccatactag ggtcccgagg attgttcagc aaatcagcaa gtacatctaa atcttctggc
     2641 tgagtttctg ccgtaccctg tatctctatg aaaagccctt cctcagcaaa ttctactcca
     2701 atacgtattt cagttatttc atgggaatat aaataagtaa gaagctcatc gatgattctt
     2761 tttgataatt ctttttccaa gtgtcttttt ttcctttctg acagcggtaa agttatctag
     2821 tatcggtaca aggatacctg caacgaaacc agttgaaaaa ccactgttat atagatttag
     2881 tcctccgtgc aaagtgctga cgttatggac aagagcaata tgtagtacac cagctattac
     2941 accgtaaaaa ctgccgtaaa aaccgcttat gggcgcgaga cctgtcccaa acacagctgc
     3001 aacaagcatg ccagtactgg tgatctcatg aaaagttcca aacaaagaag caataaatat
     3061 tccgactagt atcggaaaac tgttttttaa atgacaacca aaagcactga aacccaccgc
     3121 agataaaata gcaccgatga caggtccatt taactgaccg ccaaccagca aaacataaga
     3181 agacagcatc agaccaacta agcctatgtt gatcaaagta gcaccaatac cagaattagc
     3241 aatgaaatca gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg
     3301 aaaggaatta taatagaaac caattccgac tgtcagcgaa aaaaagccaa aaataatcca
     3361 aatgagagga aagtgatatt cattgaaaac caatgtattt tcgacgattg aatagctgaa
     3421 taacctcaaa acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc caatattata
     3481 aagtgtgaat ccacgatgga aaactaaagt ttgagcagct aagggcggaa gaagtagacc
     3541 aattgctatg ccagctaaat tccccaataa aatacctatc agtaaaggaa aacgcatacc
     3601 aaaagtgata taactaacga ccggtgataa tgcgctacca aacagtccga tcatgatgta
     3661 ctgagaaaaa ggtttattga caaatacagc gtatagatac acacctaaaa tgattgagat
     3721 agaattataa aggtttttgc caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa
     3781 taagggaccg tttaaaatca ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa
     3841 aatccctaca tttatgaatg cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag
     3901 attgcttggc gaggtaagaa tagttagcaa tccactccaa ttatcagtag gagtattaga
     3961 aagcaagcca ataaaaagaa aaaaaacacc tgtaaaacca agataaaaat actttctttt
     4021 ctgtgaaacg attcgttttt gaggatgaat ataaataacg tcagtttcgt tttccattac
     4081 acaccttctt tttcattttc tttaactata acttgaaatg aaagatttgg acaactatca
     4141 tttaggtcaa aactaaaaat gtaagggaac ccattgaatt taaccaaatt tttttaattt
     4201 tgcaaaaaaa agttaaagaa gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat
     4261 gtttgcaaaa aatgctaaaa atagggtaca ataaaacaac atatactgca gtaaagaagt
     4321 gggggagttt taatgagaag aaaagtctat accaaggatc aaattctaaa agctgcttat
     4381 gatgttgttg caaaggaagg gtttaaagga tttactgcga gaaatattgc taaaaagatg
     4441 gggatttcga cacagccgat ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg
     4501 ttgctggatt ctatctttga acaactaaaa gaagatgttt tccctattga acgtactggt
     4561 gataaaatca ttgatctcgg tttgaactat atctactttg caaaaaagca tcatagtctt
     4621 tacatggcat tgtacattga tgaacatggg gacggaaaga aaatgcatga tttttcatac
     4681 aattatttcc gtgaaatagt taaagaagat gaaagataca atcaattatc ggatgaatat
     4741 gtcgatgctc tgcatactgg tacatgggta gcagtgactg gcttagcgtc attgatgtca
     4801 tcaaacatca tgcatccaac agaagaacaa atcattgcac tgatcaaacg gacaattgac
     4861 tcgattttag cattagataa tcctgaaata atcagatagt gttctatcag tatgaatttg
     4921 atcggtaagt ccgttggctg caaactgtca atggactttt ttgctaaaaa aagcgatttc
     4981 tgttatttat ctattagaaa taggcttgac atttcgtgaa aaaagtttaa aaatagtact
     5041 tacggaaggt tggcagagtg gtaatgcacc ggactcgaaa tccggcgaac cggctaatac
     5101 cggcgcgcag gttcaaatcc tgtaccttcc ttatatcaac gattagaggt tgtgaagaag
     5161 ctgtcctgca tcaagtaata agaacggcca aacaaaaata gcttttccta ttttttcgtt
     5221 tgattgggct tattaccgca gatgcaagct tttgaacacc gtttgttcgg aataaggggc
     5281 tgtgacaaga cttttgtcac agccccttat tgttgtactt ttaacctaca atattttttg
     5341 attccccctg agtactattt tttataaatg ctagtaaatc aatcttcttt ggcctttttt
     5401 tgataaaaaa aatactagat gtaagataaa agtgtaaaaa cgatttgttt taggagggga
     5461 aacgatgtgt accgcaatca cttatgcaac gaaagaccat tattttggaa ggaattttga
     5521 ttatgagatg tcttataatg aagtggtcac tattactcca agaaattatc gtttcgattt
     5581 tagaaaggta aaaaatttag ataaacatta tgcaatgata ggaatagcag ctggggtatc
     5641 caactatcca ctctattatg aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa
     5701 ttttcctggc aatgctgatt ataaagaatt acaagaagga aaagataacg ttgcaccatt
     5761 tgagtttatt ccatggatac tagggcaatg ctcaaccata gacgaagcta aagaattatt
     5821 ggctactatc aatctagtca atatcgattt tagtgaaaaa ctacccttgt cacctttaca
     5881 ctggttatta gctgataaag aaaaatcgat agtaattgaa agtatgaaag atggacttca
     5941 tttatatgat aatcctgtcg gtgtgttgac aaataatccg ccatttgatt atcaattatt
     6001 taatctgaac aattatcgct cgctatcaaa tggaacacca gaaaatcatt tttcaaacca
     6061 gatcagcttg aatgtttata gtcgtgggat gggaggattg ggcttacctg gagatctctc
     6121 ttctgtttct cgatttgtaa aagccacatt tactaaaatg aatgctgcat caggtgattc
     6181 agaatctgaa agtatcagtc aattttttca tatactaggc tcagtcgagc aacaaaaagg
     6241 agtatgcgat actggagaaa gaaaatacga atatacgatt tattcttctt gctgtaacgt
     6301 tgataaaggg atttattatt atcgtacgta tgaagacagt caaattactg ctgtaaatat
     6361 gaataaagaa gacttggatc gtcatgaatt ggtcagttat ccagtcataa aagagcaaca
     6421 gataaactat gtcaattaat taatgaatag atccgtttgg tatcatattt tctgatacag
     6481 tcataattag acacgatctt taggtcgtgt ctttttttgc tggttttttt ggaaatagca
     6541 tccactaata tttttttaac acgaagagaa taaggtagcc tcaaaaaaga aaacatggta
     6601 aattagatat tgggacttta ttttctgtaa aaacagaaac tttcatcttt tgttttcacg
     6661 tgacgaaatt gtaagagaga aaatattcgt ccgctattta ttctatgata agatgcatat
     6721 gaatgaaata aacagtaatt ttatattagg atgtgaaagt taaatgtgcg gtattgtagg
     6781 ttttgttaac gacaaggaca acaaaaaaac aattattaat acaatgatgg atcgaatcgt
     6841 tcaccgtgga cctaatagtt caggagaata tatcgataaa catgttgctt taggattcag
     6901 aagattaagt attattgact tagagggcgg tactcagcct atttacaatg aagatcggac
     6961 aaagatcatt atttttaatg gcgaaatcta taattaccag cctttgagag aagaattgat
     7021 tgcagctggc cacgtttttc aaacacatgc agatacagaa gtccttttgc atggttacga
     7081 agaatgggga acagaattat tgcaaaagat ccgcggaatg tttgcttttg ctatttggga
     7141 taatgaaaag aatgagttat tcggtgcaag agaccacttt gggatcaaac catattatta
     7201 tgcagaaatg aacggaacat ttatgtttgg ttcagaaatc aaaagttttc tgcctcatcc
     7261 tgattttaac aaagaattga acaaagaagc gttgaaacct tatatgactt tccaatattc
     7321 tccattaaac ggcgaaacat tcttcaaaga tgtctatcgt ttgcctgaag gccattatta
     7381 tacgtataaa gatggaaaaa tagacattca gcaatattgg gatgcagatt ttgaaacaaa
     7441 agaaacgcat tcccgacaag aatggatcga aaaaatcgac gaaaccgttc aagcttctat
     7501 tgaagcacat acggtcagtg atgtagaagt tggttctttc ttatccagcg gggttgattc
     7561 cagttatgta acctctgtat tgaaaccaga tcattctttc tcaatcgggt ttgatgataa
     7621 aacctataat gaagcgattg aagccagaaa actaaccgaa ctattggact tagataatac
     7681 agcagcagtg atcgacggag acatgtcgtt caaagcattc cctttgatcc agtatcactt
     7741 agatgaacct gattcaaatc cttcttgcgt acctttgtac tttttagcaa atcttgcttc
     7801 acaaagtgtg cgggtagtcc aatctggaga aggggcggat gaactgtttg cgggatacca
     7861 aacgtatgga ttccatacga attcaaaatt catccgtgtg attgctcaag gattgaaaaa
     7921 attaccaaaa ggaacacgct ataatttagg acgtaaaatc ggaaaaatga aaaatttcca
     7981 tggacggatc catttatatg aatcacttgc tcctgcaaaa gaatatttta tcggtcatgc
     8041 tcgagtgttc gaagaatcag aagcatcaga agtgttaaca ccaaaatatc aaacagcacc
     8101 atctgtggat gaaatcatga ctgttcatta cgagaaaaca gaaggaatca aagatgaagt
     8161 caataagatg caatatgtcg atcttcatca atggatgcca aaagatatct tgttaaaggc
     8221 agataaactt tctatggcaa gttctttgga agtccgagta cctttattgg atatcgaagt
     8281 catgaaattg gctcaacaaa taccaagtaa gtttttattg aatcaaaaca acacaaaaga
     8341 tattttcaga caagctgcga acaaacatct tccggaaaaa tggtcgaatc gtgtgaaatt
     8401 aggattccct gtgccaatca aagcttggtt gaaagaagaa cacggctacg aacaagtcaa
     8461 agcattgttc gaagcagatt ttgcaaaaga attttttgat caagaaaaaa tcatgaagtt
     8521 gctagatgac catcacgaag gacgtaaaga agagcaaaga aaaatctgga caatcttcag
     8581 tttcttgaca tggtataaag tctactttgt agatgaaacg attccgcaag cagaagcaat
     8641 cgactatgtg actgtttgat gaaaatcaaa tatcaataaa aaagaggttt tctcctaccg
     8701 tttaacactg agtgataaga aacaatttca aaaaacagct cttcccgttt tatagatatt
     8761 atgacttatg gttcggagtt ggcagaggaa caccattgtt tcgaacataa gggggttgtg
     8821 acacgatttt cgtcacaacc cctttacata tgttcaataa tcgatttctt tttcttttgt
     8881 cccatctttt ttataaatga acaaagttga ttctttgttt tgtgctactt gctttccttt
     8941 ttcgattgct tctttttttg tatcaaaatg gttgcttgcc ttttttgccc ctttagattt
     9001 cacgatccat tgttcttcct catattctac gatcacatct gaatcaagca gttttccagc
     9061 acgaggattc gtatcgtgtt catccgtctt gcttggattt ttttcttttt cgaaagttcg
     9121 tttttctgat tcactagcat cttcatacca tttttttgct tgatcaatgg caattgggat
     9181 cgcacggtta tcagggtacc cttcatctaa taatgcattt gcgatgtcga ttgctttttt
     9241 tcttgtcaat ttatctaagt ttttcattga tgcaggaaag tccttcatgt tccatggcat
     9301 attagtatcc ccctttatta aaaaacataa tctatataaa caagtgtaag ggtatttata
     9361 caaaaagaaa aagattttgt ttgagaagta aaaccgttgt atttatcgtc aagtagtttt
     9421 acttaacaaa atcgtgaaaa acactagcaa tctagaaaaa atcatggtaa gataatcaag
     9481 tctgagagtc atcttagagg attcttgtaa aaatgggagg gaaaaacatg cgcgtaaaca
     9541 tcactttaga gtgtacttct tgtaaagaac gtaactatct aacaagcaaa aacaaacgta
     9601 acaatcctga tcgtttggaa aaacaaaaat attgcccacg cgaacgcaaa gttactttgc
     9661 accgtgaaac aaaataatgt ttttaaagtt ctcaattcct tgctggtcaa agaattgaga
     9721 ctttttttat ctcaaaacat ctaaaatcat aaggagcgga taatatgttt atttccatgt
     9781 gggcacaaga taaaaatggt ctgatcggca aaaatggttt gttaccatgg cgattgccaa
     9841 atgatatgcg tttctttcgc gaacatacaa tggatcgaat tttagtgatg ggaagaaaga
     9901 cctatgaagg aatgggggat ctttcacttc cttatcgcca tatcatcgtg ttgacaacgc
     9961 agccaaattt tcgcacgaaa gaaaatgcgg aagtaatgca ttcgatcgat gaacttttgt
    10021 cgtatagtca aacgatctca gaagatatct atgtttcggg tggtagtcgg atttttcaag
    10081 aacttcttcc ttacactggt cggatctggc gaacattgat cgatagcacg tttgaaggcg
    10141 atacgtatat tgggaatatt gatttttcag acttcgccct tatagaggag tatgagggga
    10201 taacggatca agaaaatcta tatgcccatc gttttcaaaa atgggaaaga gtaaaaaata
    10261 gggttgcttc aaaagaagaa taaaatctat gcgtctagtt tgttaataag aaaaaaagca
    10321 tatcacgact aatcgtgaag atgctttttt tagttaaaat caatttagtt tttctttgat
    10381 atcattgacc atttcctgac tggcaatagg tccgttatat agccaactag attcaggtcc
    10441 gaattgcagt acatgattgt ctgtcactgc tttaagattc ttccagcttg gttcattgaa
    10501 catggcagca tctttgtcac tgttcacaag gatgatgtag tcagcatcta attcagctag
    10561 cttttcagag gaaacagcag accagtcaga agtggctttt ttggagacct cttccactag
    10621 atcaggaaca ccaaattcca gatcgtcgta tacgattctt ccacttgacc gatttttgct
    10681 gaccataaag gctgagttgt tggtcaccca caacactgca gctgtcttgt tctcgatttt
    10741 atctgctagt tcttctcgag ttttcttgat ggatttttga taatcggaaa tgatcttttc
    10801 tgcctctttt tcttttgcca aagctttgcc gacatctttt agttgttctt cccatgtgac
    10861 atctgttccg ttttttacaa cataagttgg cgcgattttg ctatattgtt cgtatttccc
    10921 gccttcgaca gtagcggaag aagaaatcaa cagcaaatca ggctcaaagc ttaacacttt
    10981 ctcataagga aggtcataag aaatcgttgg gacatccttt aaatcatctt gcagataatc
    11041 ttgaatggaa ccactgccaa cagtccactg ggcgataggt ttttccccta gtgcaatcag
    11101 atagtcttct aagtaactgc cgatgatccg tttgggcgcg tttggaatct ctactttatg
    11161 tccaagtgta tctgtgactg tatgagtact attggatgaa gtcgtcgaat tggcagttgt
    11221 agctgtttct ttatctgaag agggggagca agcgctcagt actccagcca gcagcaatat
    11281 gctagataat gtgaatactt ttgtttttaa tttcatttct ttcctcctaa cataattgag
    11341 aatctttctc attggttatt attcctgata tttcgaacgc tgtcaaccgc ttcatttggc
    11401 tatagatatg aaagtagcgc ttgacgaaat gttgaaacag cgtcataatt gagaatgatt
    11461 ttcagcgaaa ggatgggtga aatgacgaga ttaagagcag ctgacattgc agccggctac
    11521 gaaaacaaaa aaatcattga aaacatgtct gttacgattc ctgaagggaa aatcactagt
    11581 ttgattgggc caaatggaag tggaaaatct actttattga aaagttttac ccgtatacta
    11641 cctgtgaaat caggaaagat cattgtcaat aactctgata tctctacata cagttcaaaa
    11701 gaacttgcaa aaaaaattgc attattagca caaactagtg aacaccctac aggtatgaca
    11761 gtagaagaag tagtatctta cggacgatac ccttatcaaa aactgttcag tggcatggac
    11821 acgcaagatc atgaagcaat cgagtgggcg ttggatgcta caggtctagc tggatttcgt
    11881 atgcgggata tcgcaacgct gtctggtgga caaacccagc gtgtctggat cgctatggcg
    11941 cttgctcagg aaacagatat cttgatcttg gatgaaccga caacattttt agatccggca
    12001 caccaattag agatcctaca gttattgact gaaatcaatc aaatgaaaca aacaacgatc
    12061 ttgatgtcga ttcatgacat taatcatgct tcacgttttt cagattattt attagcaatg
    12121 aaaaacggta agttgatcac agaaggtaca cctgaagaag tgatcacaaa agagtgtatg
    12181 aaaaaaatat ttgagattga tgcaacgatt gtcacgcttc caagaacaaa taaaccagtt
    12241 tttctaacct atgatttgtt gccaaaagag caggaggatc aattatgagg actatgaaga
    12301 aaaatagact cctgcttttg gtctgttttg ttcttttgat tatttttttt gttgctcttt
    12361 caaatggcgc agcgactgtg ggaaaagcag atttttggca ggcgttgttt gattttgata
    12421 gatcaaaaca aagtcaccaa atcatacgaa atattcgttt gcctcgtgtg gcgggagcat
    12481 ttcttgtagg gagttgtttt gcattgagcg gtgcattgat gcaaggagtg acaagaaatc
    12541 ctttagcaga ttcaggctta ctaggaatca acgcgggtgc ttcgttggca atggccctta
    12601 gttttgcttt ttggcctaaa gcttctgatt tttctgtgtt tctaatgtca ttggcaggtt
    12661 cttttgctgt aactttgctc gtatttggtt ttttgcagtt ttcgaaaatg ggcatgaatc
    12721 ctttacagct gattttagct ggcgtagcta ttagttcttt ttttactgct atcagccaag
    12781 cacttaccca attctttaat ctgcagcagg atcttgcttt ttggtttgta ggcggagctg
    12841 ccaatatcac atggaaacaa ttagcagtat tgtttccttt atatatactt gctgttttgg
    12901 tttctttttc attaggaaaa gcaatatctt tgattcattt gagtgaaaca catgcattag
    12961 ctcttggaag tcatcctaaa cgaacgcggt tgattgtttt ctgtctagtt gcagtgttag
    13021 cagccattgc cgtttcgtta gtcggacctg tttcatttat tgggctgttg gttccgccag
    13081 ttgttcgcgg aatggttgga acggactatc gttatgttct acctgtctct tttttagttg
    13141 gcggtggatt ggttatgttg gcggatttta cagcgaggat gatcaatccc ccttttgaaa
    13201 caccttttgg attagtcatt gcattgattg gcgttccgtt tttactgata caagtaagga
    13261 gggaacaacg gtgaaaaaaa tatattggac gttctatttc ttgctttttg tacttgtcat
    13321 cctgtctata ggtagtttaa tgattggtac gccttttatt tacctggatc agctttttgc
    13381 cacgttcata gggaaagaat catcttcttt ggcgctgatt gttagagaat ttcgagttcc
    13441 tcgattactg atcagtttat tagcaggagc ttgtcttggc gtttctggtt atatcttgca
    13501 aggagtaaca agaaatgaat tggcagattc tagtatattg ggcattaatg ccggagcagg
    13561 atttttagtg atgctttatt taggtttctt ttcgcactat tcgctgccgt ttctattgct
    13621 agttgtagcc tttctaggtg gtgttttagc tgcttgctgt gtctatcttg cggcctatga
    13681 tcgaaatggc tttttgggta tgaatcgaat cttattgtcc ggtattgccg ttaatgccgg
    13741 cctttctgca ttaacattgc tatgcacgat ccaattatct aaggaaaatt atggatttgt
    13801 gaatgcatgg ttagcgggat caatctgggg tgcgagctgg ccttatgttt gggcattgct
    13861 tccatgggcg gttatcttaa ttccgctagg aggactttat gcacagagga ttgtattgct
    13921 atcatttggt caagaacgcg cgcaaggatt aggccttaac gtggctaaaa gtcaaaaaat
    13981 ccttttgttg ttagctgttg ctttggcaag cggtagtgta gcggtgacag gtagtttatc
    14041 ttttgtagga ctactagctc ctcatgctgc gaagctagtc gtacgtaaag aaaataactg
    14101 gacctttgtt ctgagcggtt tgttcggttc catttttctg cttagtgcag atatggttgc
    14161 tagagtgatt ctactaagtg gggaagtacc tacaggaatt ttgattgccc tttttggtgc
    14221 gccatatttt ctgtatgcac tttttatcag acaaaaacac gcgctgaaca gttgaaattc
    14281 attaaaaaag gaaggccaat catttctagg tcttcttttt attctattta tgcatttatt
    14341 tgaaagttcg attgctattt ttcttttttt cctataccat tttatgagag aaggaagggt
    14401 gagtttttgg accaattgaa agtaagatta cgccggaaag gaatcgaaaa gctgaaaaat
    14461 ctggccaaaa gtggcaagaa aaaggaaaaa gaacagcgaa tctataaaca gctattcgcc
    14521 agcgagtact ggaagtcagc aaaagtgata gggacgactt tatcgaatga atttgaactg
    14581 gatacacagc cggttatcca acgagcagaa aaagaacaaa agaaaatagt tgtacctaaa
    14641 actttgccga aacgtcagat ggcgttttat gaaatggatg aacagtccgt tcttgtacgt
    14701 tcgagttttg gtgttcttga gccacaatca gagaagctct atttgccgga gatgatcgat
    14761 ctcctcattg taccaggtat tatctacaat agagccggtt atcgaatcgg ttttggcggt
    14821 ggttattacg accggtattt aaataatttt cataaaaaaa catgtagcct tgtttttcgt
    14881 gaacaattga atgaacaatg gcaaccggag agctttgatc agaagatcca acaattgttt
    14941 attgactaaa atgaaatggg aggttttcat gaattatcaa cagcaaataa aattgcgtgc
    15001 atggttaaga cgaccgttat ggacctatgc ttttttaggg atacagacag cggtcttcct
    15061 catgatggaa ttatttccgc gacttgagat cccttattat gccggaatgt atggtcctta
    15121 tcttgttcac ttcaatgaat ggtggcgttt agtgacacca atcttcatcc attttggact
    15181 gatgcatttc gtaatgaact cactgatact gtattttatg gggcagcaaa tcgaggcaat
    15241 ctatggccat tggcgttttt tcttgattta tatgttcagc gggataatgg gaaatacagc
    15301 aagctttgct tttaacgaag cgaatgttct gtctggtggt gcaagtactt cgatattcgg
    15361 tttgttcggt gcgttgttta tcttagggtt tcattttaaa cataacccag cgatccagca
    15421 gctagtcaga cattttcttt tatttattgt gttgacattt gtttttggtc ttctggatac
    15481 ctctgtagat gtttggggac atgtcggtgg gctaattggc ggattagtgt tagggaatgt
    15541 tttgggtcta ccgaaacaac agacaagcta ttcgatacac caaaggattc tttctacact
    15601 agtttttgta tttctttttg taatatgtat tttactgggt ttaaaaaaat atggtttact
    15661 tgtataatat gaaggaagtg cttaaagatg gagagcttat atgatgtaca gcagctgttc
    15721 aaaaaatttg gcatctttat ctatgtgggt gcacgcatct atgacattga attaatgact
    15781 atcgaattgc gtaatctgta tgatagccgt ttgattgatc atgatacata catgacagcg
    15841 tggcgcatat taaaaagaga gcaccgaatt gaagaaagcc gaaagaaagg aaatttgtaa
    15901 tggacaagaa gattattggg attgatttag gtggaacaac agcaaaattt gcaattttaa
    15961 caccagaagg agagattcag caaaaatgga gcatcgatac caatattttg gatgatggta
    16021 agcatatcgt tcctgaaatc attgaatcaa tcaatcatcg tttgaattta tataatatga
    16081 aagccgaaga ctttatcgga atcggcatgg gtaccccagg aagcgtggac agcgaagctg
    16141 gaacggttat tggtgcgtat aacttaaact ggacagaagt tcaatttgtg aaaaaactga
    16201 tcgaggcagg aacaggaatc aaattcgcta tcgataatga cgcgaatgtt gctgctttag
    16261 gtgaacgatg gaaaggtgct ggagaaaacg atccagacgt tgtattcgtt actctaggaa
    16321 caggtgttgg cggaggtatc gttgctggcg gaaatttgat tcacggcgtg agcggagctg
    16381 gaggagaaat cggacatatc actgtggatc ctgaagggtt cgaatgtact tgcggtaaac
    16441 gcggatgttt agaaactgta tctagcgcta caggtgtggt acgcctaggt cgtttcttgg
    16501 cagaagaata cgcaggggat tcaaaactga aagcaatgct agacaacgga gaagaagtaa
    16561 caagtaaaga tatctttgaa atggctcaag aagacgatcc gtttgcattg atggttgttg
    16621 atcgcgtatg cttctactta ggtttagctt gcggaaactt ggggaataca ttaaatccgt
    16681 caagtatcgt gttaggtgga ggagtatcgg cagcaggaga atttttacgt agccgtgtag
    16741 agaaatattt taaagaattt actttcccgc aagtaagaga atcaacaaaa atcaaattag
    16801 cagagcttgg taatgaagct ggtgtcattg gtgctgcctc attagccttg aaatttgctt
    16861 aaaaaaacgg aggcattaca tggttttatg ggtaattaat atcattttgc tattgattgt
    16921 gcttgcaatc atcctttggg aagtctatct acgagtaatg gccaaacgtt cagcaacgac
    16981 tttgacagaa gaagaatttc gagaaggaat gagaaaagca caagtaatcg atgttcgcga
    17041 aaaagatgtg tttgatgctg ggcatatttt aggtgcaaga aacatccctt acactgtttt
    17101 gaaagattca ttaggatcta ttcgaaaaga ccagcctgtc tatatttacg atcaaaagaa
    17161 aagtttgagc atccgtacag caaataaatt acgtaaagca ggttatcaag agatttattt
    17221 acttaagggt ggatatgacg gatggtctgg aaaaatcaaa agtaaaaaag tataaaacag
    17281 atgattgtat gacagcttta aaaaccgaat caatcattag attttgacca agatcacatg
    17341 aacggcgcaa tggatagttg tgccgttcat tttttttatt ttgtgctatt atctacatat
    17401 ataagctaag cgaaaaggta gaaagaagga aacaactatg atcaaattaa aacgtgttta
    17461 ccttgatgct gaaaaagagg atggctatag aatactcgtg gatagattct ggccacgagg
    17521 aatttcaaaa gaaaaagcgc agttagatct gtggttgaaa gaaattgcac ctagtaatga
    17581 agcacgcaaa acttttgacc atgtaccaga acgatttgct tcatttgcaa agaaatacca
    17641 gcaagaattg attttcgaag aagaaaagaa gcaagctatt tttaaactca gtcaacttat
    17701 gaaagaacat ccagtcatta cttttgttta tggtgcaaaa aatgaacatc agaaccatgc
    17761 tgttttattg aaaaaattta tcgaaatgga ggcatgcaaa tgaaaatagg gatcattggt
    17821 gccggaccta gaggattaag tgtagcagaa aggattctcc ataataatca ggacagacat
    17881 tctcttgaac tttttctttt cgatcccaat ggtcctggag gtcgagtttg gcggttgaat
    17941 cagccaccgg agttattaat gaattctgtt tctcaacaag tgacgctttt tacagatgaa
    18001 acattgtcca gtggaggatt ggtaacacag ggacccaact tgtttcaatg gagtaaagga
    18061 cctgctacag attatatcga acagacggta accaaaaata agcaactaat tatagaagaa
    18121 atcaatcgtc tggaaaaaga tcagcaatct actcgatgtt tatacggact ctatcaacgc
    18181 tggtttttct caaagttgca agaagaattt ccagcttcta ttcagtttgt ttattcttta
    18241 gtaaaaaaag taagcaaaag aaaagatggt tatcttttac agacagatca gcaaagctat
    18301 tctatcgatc agcttgtttt agcaagcggt cactgggaaa atgatgagac agaagaagaa
    18361 agagtgtggg tagactacgc aaagaaaaat gttttactgt atcagcttcc ttctaatcct
    18421 gcagatgtag atctttcgaa gataccgcct aaagaaaatg tctttttaag aggtctaggc
    18481 ttaagttttt ttgattatgt cggattgttt acagtatcaa gaggagggcg cttcgagaac
    18541 gaaaagggaa aactggtcta tcatccatca ggcaaggaac cgattgtata cagtggatca
    18601 agaagggggc tcccttatta ccctcgagga agaaatcaaa agcaaggcgg agcaatggca
    18661 tggccacggt tactaacaaa tgaaaacctg gagcaatggc atcagtcggg atttttgact
    18721 ggcgaaacat tttttgacta tctgaaaaaa gacgttgagt tgttttatta caagaaaatc
    18781 atcgaagaat atcacttgcc gattccaaag aaaaattttg agcatgattt tctctccctc
    18841 tcttcagagg agtgtctcgg aaagcatcca gcactaatcc cttatagatg ggattggtcg
    18901 tatttagaaa ctcctcttgc ctatcagaat gagccctttt cagaagctag ccgaatattt
    18961 attgaagggc agataaaaga agcggaaaaa ggcaattgca caggagcatt tgcttcaaca
    19021 tttgatgcat taaaagactg gcgtgatcca atccggcagg ctattgaatg gcacgttttt
    19081 tctgctaagg aatacaaaga aatactatgg ggatggttta ctcatctaaa tagcttttta
    19141 acgatcggtc cgccaatcat tcggacgaaa gaattagcag cactgataga tgcgggtatt
    19201 tttcatttga tcgaaccgcc atttgatatt cagcaaaagc acaaccgcta tgttatttct
    19261 ctacaagata aagaaatcag cagtcgctat ttgattgaag ctcgattgcc acaaacaagc
    19321 ttttctcgtt caagtaatcc tgtcataaaa aatttgaagg aaaacaaact tgttcgccct
    19381 ttttcatttt cagataatac aggaagttat cattcagaag ctatagatgt tgagcgaggt
    19441 acgaatcgcg tgattgatgc ttcaggggaa atccagtctg acttttattg ctatggaata
    19501 cctgttgaag gtgtggattg gctaactgca gcagtagcta gaccttatac agatccatgg
    19561 aatctgagac aggcagataa aatagcacaa cttatattga ctgacaaata aaaatacgca
    19621 tgatgactta tctgtttttt agaaagtctt catgcgtatt tttaaataat tattaacgag
    19681 aaacgcgttt acgcattgct tttttacggt tttcttcgat cattgcttct ttttcgtcaa
    19741 ttggatcgat cactgttttt ttcacagaag caaccaaacc agcaacggca gcggctgttg
    19801 ccagacttcc tactaatatt ccagatacaa attttttcat tgaatccacc tcatttatta
    19861 tttgttatat ctattatgga acaaaagaga aaaaaaggct agttttacgt ttgtttgtaa
    19921 acctttgcaa aaaatccttg aaaatgttta ctaaatgttt tattataaat ttaccatatg
    19981 aatttacgaa attttacttt gtttgttttc accataaaag taaacttcgt tttttaaatt
    20041 atatagaatc aacaaacaca attttactaa tcaaaggagt gttttttatg acaattttag
    20101 tactaggcgg agccggttat atcggttcgc acgctgtaga ccagctagtt caaaaggggt
    20161 atcaagtagc tgtggtagat aatttgctca caggtcacaa acaagcagta catccagatg
    20221 ctcattttta tgaaggagac atccgggata aagaatttct acgttccgta tttgaaaagg
    20281 aaccgattga aggagtaatc cattttgctg caagctcttt agtaggagaa tctgtcgaaa
    20341 aaccattgat gtatttcaac aacaacgtat acggtatgca aatcttactt gaagtcatgc
    20401 acgagttcaa cgtgaataaa atcgtattct cttctactgc agcaacttat ggagagccga
    20461 aagagtctcc aatcacagaa gatacaccag ctaatcctaa aaatccgtat ggtgaaagta
    20521 aattgatgat ggaaaaaatg atgaagtggt gtgaccaagc atatggcatg cgctatgtcg
    20581 ctttgcgcta cttcaatgta gccggagcaa aagcagatgc atctatcgga gaagatcata
    20641 caccagaaac gcatcttgtc ccaatcattt tacaagtagc tcttggtcaa agaaaagcct
    20701 tggctgttta tggagacgat tatgatacac cagatggaac atgtatcaga gattatgttc
    20761 aagtagaaga tttgattgct gcacatattt tagctttgga atacttgaaa gaaggaaatg
    20821 aaagcaactt cttcaattta ggcagcagca aaggatactc agtcaaggaa atgctagaag
    20881 cagctagaga agtgacagga aaagaaattc cagcagaaat agcaccaaga agagctggag
    20941 atcctagccg attagtcgct tcaagtgaaa aagcgagaga gattctgggc tggaaacctg
    21001 aatacacaga tatcaaagcg attatcaaaa cagcatggga ttggcatgtc agccatccaa
    21061 atggttatga agaatagggg aatgagatat gtcaatcagt caaacgattt gtgattttgc
    21121 cacacttgcg atcgaagctg gcggatggat ggaaatggac cgcttatatt tacagaatcg
    21181 tcttttaggg atggtaggag aagatgctct agaaagcatt gaagtaagac cagttaagga
    21241 agaatctgta gacttattgg atcagcttgt cgctaaggca agagaaaatg gtgttctttc
    21301 agaattgcca gcagaacaag agatttttga agcgcaatta atggattttc tcacaccgcc
    21361 accttcagtc gtcaatgcat tctttgcaca gcactattcg aaaagcccag aggaagcaac
    21421 tgagtatttc tttaatttaa gtaagaaaaa tgactacatc aaaacaagag ccatcgcaaa
    21481 aaacatcaaa tttccggcag agtcggagta tggtgaactg gaaatcacaa tcaatttatc
    21541 taagccagaa aaagatccaa aacaaatcgc agcagaacgc gaggcggtaa aagtagacta
    21601 tcctacctgc atgctttgca tggaaaacga aggatataga ggtcgcttga actatccagc
    21661 ccgcaccaat caccggatta ttcgaatgaa tctagacggt gaaagctggg gattccaata
    21721 ttccccttat gcttattaca atgaacattc gatcatttta tcagaacaac accgcccgat
    21781 gaaaatcaca aaggaaacat ttgctagact gcttcggatt actgaagtct tgcctcatta
    21841 ctttgttggt tcgaatgctg acttaccaat tgttggagga tcgattcttt ctcatgatca
    21901 ttatcaggca ggacgtcatg aatttcctat ggcaaaagca ccgatagaaa aacttatcaa
    21961 actagaagat tatccgcaag tcatcgcagg agtcgtgaaa tggccgatgt ctgtgatccg
    22021 tttgcaatca acagagaaaa acttgcttat tgaagcagct aactcagtat tggaaaaatg
    22081 gaaagcatat tcggatgaaa cagtatctat cagagctttt tcagcagatg gtacaccaca
    22141 ccatacgatt acgccaattg cacgaagaag ggacaattac tttgagttgg accttgtcct
    22201 tcgagacaac aatgtttcag aagaacatcc tgatgggatt tttcatccac ataaagatgt
    22261 ccaacatatc aaaaaggaga acattgggtt gatcgaagta atggggcttg ctgttttacc
    22321 acctcgtttg aaaccagaac tagtagaagt agagcatttc ttattaggag aagaaaacca
    22381 gatggctgaa tatcatcgtc cgtgggcaga acaattaaaa aaagaacatc ctgatttgac
    22441 gaaagatacg gtcaccgatg tcgtacaaaa atctgtaggt caggtttttg cacgtgtact
    22501 ggaagacgct ggagtattca agcgagatga aaaaggacag gcagcgtttg aacgatttac
    22561 tgcgatttta taaacgtaaa aaaggaggag agacaaatgg caacaattaa agacattgcg
    22621 cagttggcag gagtttctcc tgctactgtt tcaagagttc tcaactatga tcctgatctt
    22681 tccgttggct tagccacaaa gcaaaaaatt tttgaagcag cagaacgctt aaattatacg
    22741 aaacatcgta aaaatgcagc aaatactaag caaacgcttc gcctgatcca gtggtacgac
    22801 gatacggaag agcttgaaga tctttattat ttatctattc gcctaggaat cgaaaaaaag
    22861 gcagaagaag caaacgttac tttattgaaa gaaacgtggg attccattag tgatcttccg
    22921 gcagatggaa cgatcgcact aggaaaattc gacgaagctc agttattaga aatgaaagga
    22981 tcccaaaatg ccttgctttt ggtagattct gacggcactt ctcaaggaat cgactctcta
    23041 gttgtcgact ttaagtcaag tgttcaaaaa gtcattgaac actttgtctc ccataaagcg
    23101 caacatatcg ctatgcttgc tggaacagaa tatacgaaga aaaaccatca gcggctaaaa
    23161 gatcccagaa ttatggcatt tcaacaagtt ttggcagaaa aaggctacga cgaaggtcca
    23221 attatcgaag cagatttttc tgtcgagtca ggatatcaag cagtgaagca gtatttaaaa
    23281 acgaatccgc atgttcctga tgcgcttttt gcagctaatg atgcattagc gattggcgca
    23341 ctgaaagcga ttcaagaaaa aggtttagtg gtaccagaag atatttctgt gatgggcttc
    23401 aatgatgtca gtgtagcgaa atatgtaacg ccctcgcttt ctactgtaaa agtttatacc
    23461 gaatggatgg gagagctagc agtggagacg attcttcaat taagtaattc caaagcaccg
    23521 gtttcaagaa agattattgt ggaaacagag ttgattattc gagaatcaac gaaataaaag
    23581 atggaaaagc tgtgggaaaa tggagtgata ccgcagcttt tttgtgtttc aggaaggggc
    23641 aaaatttgcc cgccagcaga taatcatgta ttattgtact agttttgcct ttggtatttt
    23701 catcctcaga ataaaaaagt atgctacaat ggaaggcgag aatgtcatag cagaaaggat
    23761 caaatatatg attacattag gaatagatac tgcaaaccaa actttggcga tcggtgtggt
    23821 agaagacgaa caaattcttg gacaaataca aacaaatatc aaacgcaacc acagtgtaac
    23881 actgatgcct gcaatcgacc aattgtttgc agatcttcaa ataagtccaa aagacattga
    23941 ccgaatcgcg gtttctgatg gtcccggttc ttatacaggg cttcgtatag gtgtaacgac
    24001 agcaaaaacg attgcttata cattagataa agagctagta ggagtatcta gtctgaaaac
    24061 catcgcggct aattgcatcg gtgtggataa tattattgtg cccatgtttg atgctcgaag
    24121 aaaaaatgta tacgctgggg cataccgctg gaaagaaaat agattagaaa ctgtacttga
    24181 agatgtacat atttctgctt ctgatttgtt cacacaacta caaaagctga atgaagctat
    24241 ttatttcgtc gggagtgatt gtcacaaatt cttctctgaa ataaaggaaa tattgcctag
    24301 tgcgatgatt aatacagttc cagcatggga tattccaagt ggtgtaactg tggctcaatt
    24361 aggtgcttta gttgaaccag ttgcagagaa acaagctttt cttcctcgct atttaaaacg
    24421 agtagaagca gaagaaaaat ggttggagac gcacacacct ggagacgaaa gttatgttga
    24481 aaaaatttag agcgtatgcc agaagctttt ttcaagataa aatgatttat acagaaaaaa
    24541 cagtcgaaca aatcgatcgt cgttacgtac taagagaatt gactacagaa gatatcaaag
    24601 agctgctgga tgtcgaacga gaagtctata atggcgaatt gccatggaca aaaagtgcct
    24661 ttatatcaga attgaagtca ccgcttcctc atctatatct aggtatcttt gataatggaa
    24721 gaatgattgg ttttatcggt tcaagagcgt tcggtttaga ttgccatatc acgaatattg
    24781 ccgttcgacc ggcatatcag cgtaaacgaa tcggttcgct gcttattgac gaaatcgaga
    24841 actttgctat aatgaatcgc tgtgagacga tgtcattaga agtgcgtatg agtaatcaag
    24901 atgcacaaag actttatcgg aaattggggt ttcaagcaac aacgatcaaa aaaagatatt
    24961 acactgaaac aaaagaagat gcgttggata tgataaaact tttagaaaat gagtgagatt
    25021 agttgatttg cagaaaaaaa gattttgatg aagcgtactt ggcagaatgc gtatggatga
    25081 ttagcgaaaa tgcttatcca catggttcac cttggacgaa aatgcagttt ttggcagaca
    25141 tccaacagcc tcactctgat tatctgcttt tagctgaaga taaccagatc tgtggcttta
    25201 ttggatactc gaaagtaata gatgaagtcg agatcacaaa tattgctgtt gcagtttcag
    25261 aacaaggaaa agggcatgca cggcatttgt tacaattact gattacggaa caaataaaag
    25321 attcgctgca tgttttttta gaagtacgtc tatctaatga agcagcaaga aaactttatg
    25381 aatcagaaaa gtttcgtata ttaggtaaac ggaaatcata ttatcgcaat ccgacggaag
    25441 atgcgatcat tatgagcaga aagttaaaaa cggagacgat gaaatgaatg aagaattgat
    25501 tctggcgatt gaatcaagtt gtgatgaaac gagtgtagcg gtcgttagaa atggaacaga
    25561 gatcttatct aacatcgttg cttctcaaat aaacagtcat aaacgttttg gtggcgtagt
    25621 acctgaagta gctagccgcc atcacgtaga acaaatcacc ttatgcttgg aagatgcact
    25681 ggtagaagct ggtgtgtcgg cagaagattt atcggcagtc gctgtcactt atggaccagg
    25741 acttgtcggc tcgttgctga ttggtatttc tgcagctaag gcttttgctt gggcacacca
    25801 attgccgctt attcctgtaa accatatggc agggcatatc tatgcagccc gtctggttaa
    25861 accattccag tttccattga tggcacttct tgttagcgga ggacatacag aattagtcta
    25921 tatgcaagaa gatggatcat atgaaatcat tggggagaca agagatgacg cagcaggaga
    25981 agcttatgac aaagttggaa gagtattagg tctttcttac ccaagcggaa aagaaatcga
    26041 tcagctagct catcagggaa aagataatta ccattttccg cgagcgatga tccatgaaga
    26101 taattatgat ttcagtttta gcggattaaa aagtgcattt atcaatttgg tgcataatgc
    26161 acaacagcgt ggagaagatc tagacaaaaa tgatttagca gctagctttc aagcaagtgt
    26221 gatcgatgta ttgattacta aaacacttcg agcttgtcaa aattatccag tcaagcaact
    26281 gattatagct ggaggtgtag cggccaatca agggttgcgt gaaggattgc aggatgcttt
    26341 ggcaactaaa ctccctgagg tagaactagt gattccccct cttcgacttt gtggagataa
    26401 tgctgcaatg attggagcag cagcacatgt ggaaatgcaa aaaagaaatt ttgctagcta
    26461 tcagttgaat gctgatccaa gcttggtttt atctgaataa agaggttgtg aaaaagctgc
    26521 cctgcatcaa gtaataagaa cagcccagga aaaacggctt ctcatgtttt tgacctggct
    26581 gttcttatta ccgcgtatgc aagcttttga acgccgttta atcaagagtt cgtaagcctg
    26641 tcgtttaact ctgattcaca aggaacaatt tcaaaaaaca gctcctcatg tttatgaaca
    26701 ttatgacttg tgaccgaaga gttgacaggt acacaccgtt taatcgaaat agggctgtga
    26761 cacgtttttg tcaaagccct attttttaca tacttttaat cattattgtt attttgctat
    26821 tttttacagc ttatttatca ttatccgttc ctctgattgc cctcactgag taatcgttta
    26881 ttgtcgagga atatcgaata atctagatcg gatgaggaca agacgcttca tctagttaat
    26941 ctaacagtat tttttgcaaa cagtgaatat tacataactg aaacacacat agaaaatctt
    27001 tgagtcttgt ccagtgatct aaaactgccg gatctttcag aagttttagg gaggaaaata
    27061 agcagtgacc ctgatattta tcagggtcac tgcttatttt tgttcgcgtt attctctcat
    27121 agctggagta gtagctgtct tacagaaagt aaaacgcttt ttgtcataat ctaacagccc
    27181 ggaatcacgt agttgtttga ttacttgtcc agcagtttca cgtgtcgtgc cgctattcac
    27241 tgctaattct tttaaggtga tcggataagg aatggtgata tgaccatctt ttgttggtac
    27301 gcccaattct tctttcaaaa tgaataaatt ttgttttacc cggtcaaagg cgctagaggt
    27361 caatccgatt tggatacgca attcatgtat ttcaactact ttagaaattt tttgtatcat
    27421 atagatcaac tgttcgggat tttttcgtgc taatttttca tagtaggaaa caggaaggta
    27481 gaatgtttca atatcagtca tcgcttgagc tgtataagtg taggtctctt ctccaaacat
    27541 tcctgacata ggaaaaaggt tatttgtatt cacatagtca tagtagtaat aagttgcact
    27601 ttcatcataa cgttctaaac gaacgagtcc tttggtcaag atatacaacc gttgtctggg
    27661 atcaccatcg tcgaaaagga tctgtccttt tttataagaa cgaagaacag tatgttcctt
    27721 gatctgttgg aactcttcat ccgagaaatg ttcaaaatca ggttgatatc tgagttttga
    27781 aaagtgaaaa tcgattttat taacacgcat taaaaagtcc tctccttatt gttcactata
    27841 tttattgtca ctttttttaa agaatttgta aagttttaac ataccaacat tagaaacaag
    27901 ttttaatact ttacaaatat aatttgtt
//
LOCUS       DAFGZE010000033        27925 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.033, whole genome shotgun sequence.
ACCESSION   DAFGZE010000033 DAFGZE010000000
VERSION     DAFGZE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.033"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(424..1368)
                     /locus_tag="LTU54_001575"
     CDS             complement(424..1368)
                     /locus_tag="LTU54_001575"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL8296291.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="LTU54_001576"
     CDS             complement(1664..2608)
                     /locus_tag="LTU54_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296292.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="LTU54_001577"
     CDS             2829..4043
                     /locus_tag="LTU54_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8296293.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="LTU54_001578"
     CDS             4065..6080
                     /locus_tag="LTU54_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBL8296294.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="LTU54_001579"
     CDS             complement(6163..7536)
                     /locus_tag="LTU54_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL8296295.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="LTU54_001580"
     CDS             complement(7562..7846)
                     /locus_tag="LTU54_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296296.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="LTU54_001581"
     CDS             complement(7848..9863)
                     /locus_tag="LTU54_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL8296297.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="LTU54_001582"
     CDS             complement(10042..10347)
                     /locus_tag="LTU54_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HBL8296298.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="LTU54_001583"
     CDS             complement(10436..11467)
                     /locus_tag="LTU54_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8296299.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="LTU54_001584"
     CDS             11674..12549
                     /locus_tag="LTU54_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL8296300.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="LTU54_001585"
     CDS             complement(12701..13543)
                     /locus_tag="LTU54_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296301.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="LTU54_001586"
     CDS             complement(13635..15512)
                     /locus_tag="LTU54_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL8296302.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="LTU54_001587"
     CDS             complement(15509..16930)
                     /locus_tag="LTU54_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL8296303.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="LTU54_001588"
     CDS             complement(16933..20322)
                     /locus_tag="LTU54_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBL8296304.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="LTU54_001589"
     CDS             20579..21970
                     /locus_tag="LTU54_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296305.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="LTU54_001590"
     CDS             complement(22043..22690)
                     /locus_tag="LTU54_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HBL8296306.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="LTU54_001591"
     CDS             complement(22698..23384)
                     /locus_tag="LTU54_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296307.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="LTU54_001592"
     CDS             complement(23390..24463)
                     /locus_tag="LTU54_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296308.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="LTU54_001593"
     CDS             24685..25359
                     /locus_tag="LTU54_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8296309.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="LTU54_001594"
     CDS             25364..26704
                     /locus_tag="LTU54_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8296310.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="LTU54_001595"
     CDS             complement(26853..27839)
                     /locus_tag="LTU54_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HBL8296311.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8147 a         5272 c         5057 g         9449 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggtgcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta
    27901 taggagacga ttgttttcta caaat
//
LOCUS       DAFGZE010000034        26466 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.034, whole genome shotgun sequence.
ACCESSION   DAFGZE010000034 DAFGZE010000000
VERSION     DAFGZE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.034"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(324..893)
                     /locus_tag="LTU54_001596"
     CDS             complement(324..893)
                     /locus_tag="LTU54_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL8296312.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(967..2256)
                     /locus_tag="LTU54_001597"
     CDS             complement(967..2256)
                     /locus_tag="LTU54_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296313.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQAFAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            2610..3458
                     /locus_tag="LTU54_001598"
     CDS             2610..3458
                     /locus_tag="LTU54_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBL8296314.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPVPVR"
     gene            3472..4545
                     /locus_tag="LTU54_001599"
     CDS             3472..4545
                     /locus_tag="LTU54_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HBL8296315.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKREISFPVTVL
                     PAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKLSAHTQEWLY
                     REYDYQEEECSPLFCFWGSHSPKFEPLLDLYFSLWHLRKEKQPELLILGNRAFHGKDP
                     GHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKDRTVYLVYFV
                     EKQLMVQEKDKICSLPGGEWIDD"
     gene            4535..6706
                     /locus_tag="LTU54_001600"
     CDS             4535..6706
                     /locus_tag="LTU54_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBL8296316.1"
                     /translation="MTIKWEKWLCGVYSFVILSVGAPIFVVANQLQNSYLLILTIGVL
                     CLIVCCIPKKWVRLLLSLPVMIGCLFLYFPSKTFSLLWILQFFSSVTEEFFHLSQGKL
                     YYLPEKTAFFLIMLLIYLFITLTVDYDYLYAPFLSLMAYFMMLTVFREKDLLYEMIAV
                     VTVLFVYLAARQFFSMRLLSGNTRFQSLTTVLLICFLTISAILPLYLPKVHQSLEETS
                     EPIREYLNDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYW
                     KIENKNHYTGSGWIEDQDAPIQPIQEFPVDITSYQTVMEDKENITLTLKKQAFLPYPY
                     GNTTLTDFSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKAD
                     DLENQQTNLQLPKSLPKRIEDLAVELTKNEATLIRKVEAVEQYLKTSGNFRYSKTDTG
                     HTPKEHDYVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGN
                     EEYSILNSHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNES
                     ENMAETISSERSEVIPSEETTLTPAYKIVLFSLGVCLYVLLFFLLLFRKSLRWTSYLI
                     YLHFFASTNTIAYQKLLNHLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEA
                     EIYGRQADPVNFDKKTYLEITHIIRKTVYKKVLTKGSRFKK"
     gene            complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LTU54_001601"
     CDS             complement(6818..8989)
                     /gene="copB"
                     /locus_tag="LTU54_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HBL8296317.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHDHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKNQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9009..11195)
                     /locus_tag="LTU54_001602"
     CDS             complement(9009..11195)
                     /locus_tag="LTU54_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBL8296318.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKSKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMSSSLASIAQTIELSRVTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LTU54_001603"
     CDS             complement(11195..11404)
                     /gene="copZ"
                     /locus_tag="LTU54_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HBL8296319.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(11417..11857)
                     /locus_tag="LTU54_001604"
     CDS             complement(11417..11857)
                     /locus_tag="LTU54_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HBL8296320.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(11931..12404)
                     /locus_tag="LTU54_001605"
     CDS             complement(11931..12404)
                     /locus_tag="LTU54_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation family protein"
                     /protein_id="HBL8296321.1"
                     /translation="MGRENRPSQGRSKKSPAFRIQAKKWFLQEQQSFMVSNPSWIVEG
                     NYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLKRKINKKSRPDMPESFSEKLNRK
                     YLEFLSFIWHFEGNNEPKIQQLIQVANARNKLIILKTRREKQEFLTKLKIESKNN"
     gene            complement(12714..14180)
                     /locus_tag="LTU54_001606"
     CDS             complement(12714..14180)
                     /locus_tag="LTU54_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296322.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="LTU54_001607"
     CDS             complement(14491..16575)
                     /gene="cadA"
                     /locus_tag="LTU54_001607"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBL8296323.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKKRNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     RAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17099..17458)
                     /locus_tag="LTU54_001608"
     CDS             complement(17099..17458)
                     /locus_tag="LTU54_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBL8296324.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(17488..17829)
                     /locus_tag="LTU54_001609"
     CDS             complement(17488..17829)
                     /locus_tag="LTU54_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296325.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(17826..18500)
                     /locus_tag="LTU54_001610"
     CDS             complement(17826..18500)
                     /locus_tag="LTU54_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBL8296326.1"
                     /translation="MKKRILFIGDSITDAGRYRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIVAVDFFSGDKNE
                     RKKSEK"
     gene            complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="LTU54_001611"
     CDS             complement(19295..20593)
                     /gene="asnS"
                     /locus_tag="LTU54_001611"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBL8296327.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKITFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(20633..21823)
                     /locus_tag="LTU54_001612"
     CDS             complement(20633..21823)
                     /locus_tag="LTU54_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL8296328.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(21844..22371)
                     /locus_tag="LTU54_001613"
     CDS             complement(21844..22371)
                     /locus_tag="LTU54_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HBL8296329.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(22418..25207)
                     /locus_tag="LTU54_001614"
     CDS             complement(22418..25207)
                     /locus_tag="LTU54_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBL8296330.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDINEFLKKNEEGS"
     gene            complement(25354..25554)
                     /locus_tag="LTU54_001615"
     CDS             complement(25354..25554)
                     /locus_tag="LTU54_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL8296331.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(26006..26326)
                     /locus_tag="LTU54_001616"
     CDS             complement(26006..26326)
                     /locus_tag="LTU54_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296332.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7453 a         5604 c         4647 g         8762 t
ORIGIN      
        1 gtcaaggaca atcggaatag catacggaaa gaagttctgc accttataaa atttgtcaaa
       61 aaaactgttg ccattccaag tgaaaaaaag atcaatgaat caactagaaa actatataga
      121 agagcaaata aaaaagcact caaattgagt gcttttttaa gctcactatc actaaaacta
      181 tcactaattt tatttataag cgaacatgtt tctatcactg attttttaaa tctagcgaaa
      241 aacccttatc ttaaaggcgt tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc
      301 ctagaagtgc gaacatggtt ttcttatagc tcatgtagaa aaattttctt gatgatgatt
      361 ttatccatca attgagattg actgttttgc tctcctatga tttcagctat gattgtccga
      421 ttttgcggtt ttcttcttaa ctggtagttg attttcccag tttccatcaa ctcgctaagc
      481 atatccacga ctccgccaaa tccggtcttt ttcgtaataa aacctagtgc taccattcgt
      541 tcatctagat tttttgctgt aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg
      601 gccagctctt ccatcccttc agcattggat acgacggaag ctgttttgtt aataggttct
      661 tcgtttattt cttttggttc tttcctctcc actttcactg tttgacgaag gacatctagt
      721 tcttgcaaga gcgtttcgta ttgttcttgg gtcagcatca cgccagctac tttttcacga
      781 ttaaatatat atacgccagt tcccgcgtct cgcgcttgag caaatacatc catcggcgat
      841 cgtttcactt ctgtgatcga agatgtggga acttctaatt tctttaattc caatggattt
      901 cctcctttac taatcgcttt ttattatagc acagactgac ttgttttgat cttttttcca
      961 aaggttttat cggtttttgc ctaaccagta attgatatcg gtaagaagtc tttcgatacc
     1021 aaatgaagga tggtacagat acgttttttg atcttcgtta tggtctagtg gaaaaaaatg
     1081 atcggtgatc actagatctg cataccgagg cggcttattt cgagtacata catgtataga
     1141 gatccgtttt cgatgtctta gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg
     1201 gtgttcatag cgaaaaaaaa agacgatttt cacttcagga gggtggacat ctaaataccc
     1261 tcgttcctgg agtgttttca gtaaaagcat cccccactct actgcggggt gtgcaaatgc
     1321 ttgttggatc gctagccgaa attgatatag tttatctttg gaattgggaa atttttctaa
     1381 tgtttcctga taataacgaa cttcttctat cagccggtca tggataccaa ttacgtaacg
     1441 aagccattct tggtaaagta cttttgtcag ctttgatttg ttttcgctct caaattcgct
     1501 attcagccaa aacagcgaat caagcttttc agtcacaatc gtcaatttcc gatagtcttg
     1561 tgtctcttct atccgtttcc accgcttttc agtaatctgg ttacagctca agcgcaaaat
     1621 tgctagataa tttttttctt ttattggcaa aatctgctct tctctaaata gcaattcagg
     1681 aaacaattca aagaatagtg ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt
     1741 tctttgcttc aataattgga tgaacatctc agaaaaggac tgctctccat catctgcaaa
     1801 aaaatgcgga aaatctttca aaaagcaaaa cagcagatat cttacttttc tttctgaacc
     1861 aaagatctgt gcttttttat aagaaaaaga aaggtggtac tcgccaaacc actttctcaa
     1921 tcgctcttgc gtagaacgat cgtacttctg ctcgtaaggt tcattcaata gcagattttt
     1981 cagtaaacaa aacgggggtg attggatcag aaaaacaaac aagagataag gttcgctata
     2041 tccttgatca taaactaact gaacggtttt cttatgaaga atcagctgga tgggacagtc
     2101 gctttccgcc aaacgtttca gatgccgatc cactgtttta acatcgattt tcagttcttc
     2161 agctaaatca gcttttcgcc agatcaaatc tttcattaga ttgattagtg aaaataaatt
     2221 ctgtgcattt tttgataata gtttttgcca tctcatcgat catttctcac ctgatttcta
     2281 aacatattgt ttgatcaaaa tgggttcact cctaaaaaga taaaaaactc cctttctatt
     2341 tatttaaata cgcaaatttt cctcattatt tttaatttta ctcaatttaa tcgtattctc
     2401 atttcaaaac gaatgtactt taaaaaaaca taataaaatc actttgcttg taggatgtat
     2461 tcccttccat tacaatgaaa taaatagcaa ttttatgaga aggtgatgtg aaaatggaag
     2521 aaaaaatcga agaatccaaa caaaaaatcg atcagttgat acaagaatta gaaaaagcgg
     2581 tcatcggcaa acgttcaact atccaattga tgatcgtggc cctattagca ggtggtcatg
     2641 tcttattcga agatattccc ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag
     2701 ctgtacaggg aaccttcagc agagtccaat gtactcctga tctgctgcct agtgatattc
     2761 ttggtttttc cgtatacaat tcacaatcaa aagagtttga atttcgtccg ggaccgatct
     2821 ttaccacgat cttgttggca gatgagatca atcgcacgac tcctcgtact cagtcagcac
     2881 ttttagaagc aatggccgag aatcatgcaa cgatcgacaa caatacatac cctctagaca
     2941 atcatttttt tgtcttagct acgcaaaatc caatcgagta tgaaggaaca tatcctttgc
     3001 cggaagcaca gctcgatcgt tttttattcc gactacaaat cggttatcct tcttttgatg
     3061 acgaactttt gctactttta gataaattcg aaaaagtgtt ggataactta aatgaagtat
     3121 tgaacagtta tgaaatagaa gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg
     3181 aagtggcatc ttatgccttg cagttagtgt cagcaacaag aagccatgct gccatccagc
     3241 ttggcatcag tccacgagga agcctttcct ttattcaggc agcaaaagct tatgctttaa
     3301 tccatgggag aaattacgtc acgcccaaag atttgcagga tttagttcct tacgtgtttt
     3361 cccatcgcct gattttttac gatcgttcat tgaaggaaga cgaaaaaaaa caaatcattc
     3421 atactcttgt tgcacaaatt cctgttcctg tgaggtagat tgaggtagat aatggaaaaa
     3481 atcaaagatt atttcattaa ttttgtgtta tttctggtat atttcatcat tgtgcttttt
     3541 gctcttgtat tcaacaattc tttaggctgg ttcttgttgg ccttttttac tctttatgta
     3601 tcatggagct tgatttcatt aagcagcttt ctaaagaaac tttcggttac ttgtacattg
     3661 cagccactcg tttatcgcaa aaaaagtatg caggttattt tttccatagc aaaaaagaga
     3721 actttttctt tacctctccc tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa
     3781 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     3841 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     3901 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     3961 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     4021 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     4081 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     4141 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcacagtcc aaaatttgaa
     4201 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     4261 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     4321 ttggaagctg gcgatgagaa agcttttttc gcacacctaa aaaaatatgc aaaaaaacaa
     4381 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     4441 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     4501 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaatggg aaaaatggct
     4561 gtgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     4621 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     4681 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     4741 gtttttgtat tttccttcga agacattttc cttattatgg atcttacagt ttttttcgtc
     4801 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     4861 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     4921 ctatttgtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     4981 ggaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     5041 agcaaggcaa tttttctcca tgcggttgtt gtcgggaaac actcgattcc agtcattgac
     5101 tacagttctg cttatttgtt tcttaactat tagcgctatc ttgcctttat atcttccgaa
     5161 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     5221 gctttatgat acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     5281 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     5341 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     5401 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     5461 ctaccaaacc gttatggaag acaaagaaaa cattacattg accctaaaaa aacaagcttt
     5521 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     5581 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     5641 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     5701 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     5761 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     5821 cgaagctgtc gaacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     5881 cggacatact ccgaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     5941 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     6001 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     6061 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     6121 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     6181 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     6241 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     6301 accagcttat aaaatcgtct tattcagcct tggggtgtgt ttatacgtct tgttgttctt
     6361 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     6421 tgcttctact aatacgattg catatcaaaa acttcttaac catttggaaa cattggtacc
     6481 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     6541 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     6601 agatccggta aatttcgata aaaaaaccta tttagaaatt acgcatataa taagaaaaac
     6661 ggtatacaaa aaagttctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     6721 agaatctact tctggaatgc cgagaaccag ctgcttgagt atcgattatt cgaaaaaaag
     6781 gttgtgacac gttttttgtc acaacctttt ttcattatta tttgattttc aatgtcaaag
     6841 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     6901 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     6961 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     7021 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     7081 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     7141 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     7201 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     7261 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     7321 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     7381 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     7441 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     7501 tcacttgttg gtttttgact gttgcctcta gcccaactcc tgaaagattt tttagatttt
     7561 cggcttgata aggcttgatt tcatgttctt tcaaataatt cataatacca acagccaatg
     7621 gatgattggc attcgcttcc aatgctccta aatattgaag gatttcattt ttattgaacg
     7681 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     7741 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     7801 gtaatccgtt tttagctgct attgatgtag agcgagcaac aactaatggg atagctaaac
     7861 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     7921 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     7981 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     8041 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     8101 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     8161 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     8221 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     8281 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttt actgattctg
     8341 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     8401 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     8461 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
     8521 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
     8581 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
     8641 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
     8701 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaatttc
     8761 ccatgtgcat ggaatgatcc atgccgccat gatcgtgatc catgtgcatc gaatggtcca
     8821 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tggtccatct
     8881 ggctatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
     8941 ctacttcttt tttatgttca tgttttttct ccatatcgtt cccattcatt tttactccat
     9001 cccttctctt actttttcat gtgccgattt aagctcaggg agttcaacaa tacactgacg
     9061 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
     9121 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
     9181 gtcaccctag acaactcgat tgtttgtgca atggatgcca aactgctact catcagcgtc
     9241 acatcggctg tttccatcgc gatatccgtt ccacttccca ttgccatacc aacatcagct
     9301 aatgctaaag ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
     9361 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
     9421 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
     9481 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt ggcttctagt
     9541 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
     9601 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
     9661 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
     9721 ctgattccag cacctgggtg agctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
     9781 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
     9841 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
     9901 gtaatcgtcc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
     9961 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    10021 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    10081 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    10141 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    10201 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    10261 ccgacttgtg tgacttccat gtgtagcaga ccattcgtat tgattgtccc gccaaaaagc
    10321 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    10381 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    10441 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    10501 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    10561 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    10621 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    10681 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcga cttgattgca
    10741 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    10801 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    10861 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    10921 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    10981 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    11041 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    11101 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    11161 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    11221 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    11281 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    11341 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    11401 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    11461 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    11521 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    11581 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    11641 gatgataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    11701 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    11761 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    11821 aatcactaat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    11881 aatgtaatca ttaacttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    11941 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    12001 atcagtttat ttcttgcatt agctacttgg atcagctgct gtatttttgg ttcattgttt
    12061 ccttcaaaat gccagataaa agataaaaat tctaagtatt tcctgtttag tttctccgaa
    12121 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    12181 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    12241 ttaatccttt cctctaacgt agaagtataa tttccttcaa cgatccatga cggattagat
    12301 accataaacg attgctgttc ttgcaaaaac cacttttttg cctgaatgcg gaaagccggt
    12361 gatttctttg atcttccttg cgagggacga ttttcccgac ccactttgtc caatcacgat
    12421 gatcttcatt cttctccctc acttctcttt ggaatttggt tcgatcatac cacttataac
    12481 gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa agtcccttgt ttcgaataaa
    12541 tggtgttcaa tcagctgacc ttcagcatta gctcaaaaaa cggcaaaata tgaggagcta
    12601 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    12661 cggtgttcca gaagtagctt ctgtcacaac ctcttgtaac aagtatttct attttaaggc
    12721 tgaaccactg tttctggttc agtaatttct ttgtttttgt ctcgacgagc aattgctggc
    12781 aagatcatac ctaataagat caagacaatt ggtgtgatga tattcgtgaa taattggaac
    12841 caccatgctt taggatcagc agtataatct acttttggca ccattcctag gatacatgca
    12901 aacgcagtga aggcaaagca ccaagcacca aagataaaac cgatttttgg atttttaaca
    12961 aatttatatt cagaattgaa tttcttataa gctttattca acatcatata tgccaagaac
    13021 acccataggt aacgcattgg catgacaact gagttcaagt tcgtcaacca ttttactaat
    13081 tcattcatat ctttgatacc aaagatcggc aacaaaataa tgattcctac tagtatacct
    13141 gtcagaagat aaccattcac caatgtacct ttacgattac gtttacgtag ccagtttggt
    13201 acaaattctg gatccgcatc tgctaacaag atttttagtg gtgcatcaat tgaaaaggct
    13261 aatgcggaga tttgtccaat catgtttgtc aatgcgtaaa tgatcatcaa tgagttaccg
    13321 ataccatagt aattacctaa aatttgaaaa gcactgtatg ccccatttgc catcaagtct
    13381 ttagggatat tatcgctagc aaacagcata cccattgcga ctgagcctaa tacagcacaa
    13441 atccctacca ttgctgctaa gaagatcatc cctttaggaa attcctttgc aggatttctt
    13501 gtagagttga cataagggga tattttttcc gcccctccaa ctgcaaatac tagcatcgaa
    13561 acggttgtga aataactgaa atcaaatgtt ggaatatatg ttttcacttg attcatatgt
    13621 ggtgtggcaa attccatcgt tggattgatg aacggtgccg ttacagctaa aagaataaat
    13681 aagatcgaca tgacgaacat cgctgttccg gccagtccgc caatcacttt caaagtagaa
    13741 agtccttttg ttgataaata taaaaaggca agaaatataa taagtgaaat aactgctacc
    13801 atttgtactg acatcgtatt tacgatattt ccatttcctt ggactgccca accaccagca
    13861 atcagaattg cttgaggctt ttgtgccaaa taaggaatat ggacgaccca gtaagtccaa
    13921 gcggcatagt atgccagtcg tttattagat gtcgtatttt cgacccatga actgacgcct
    13981 cctttactat ccttgaaggc tgatcctaat tgtccaacga tcaatgcgta tggaataaaa
    14041 taaatggcca aaatcaatag ccaagaggtg acaactgata ctccttgctg agcatagtta
    14101 ttaactacat tccccatgcc ccaaaccatg ttaaatgcaa ttaacgcaac agtaaaccag
    14161 cgtaattgtt tgttgttcat tcgttctctc ctttatgata aaaatttgtt atccagatgt
    14221 aatcatattc aatttttaac aaacttacaa gacagtgtta ttttatgtgt gtattctttt
    14281 atttaataca catcgttttt tcatattttt tccatcaaaa aaataagtta ttttctttta
    14341 tttcattgac cttattgaat ttcaagctat tttgaatttt ctgcaaaatt aaaaacatta
    14401 gttattttat aatcctatct attttcttta catttttcat taagaaaagg atataaaaaa
    14461 aagaggacgt tcattcgtcc tcttcattga tcaacgttta ttttttacat gcaagcttcg
    14521 catggcattc aaaacagcta atactgtcac cccaacatct gcaaatactg catctcccat
    14581 tgatgcaaaa ccaaaagcac ctaaggaaag taccaaaatt tttatcccaa tagcaaagac
    14641 aatgttctgt ttgacgattt tcaatgtttt ctttgctaaa tggattgctt ctgcaatctt
    14701 cgatggttga tcattcatga tgactacatc tgctgcttca attgcagcat cgcttcccaa
    14761 tccacccatt gcgattccta catctgctct tgctagcacc ggtgcatcgt tgattccatc
    14821 tccgacaaat gcggtctttt tattgttact ttgtaagatt tcctctaacc gctgcacttt
    14881 atcttctgga agcaattcgc tatatatctt gtccacgcct acttgcttcc cgatatgatc
    14941 agcaattttt ttcgagtcgc ccgtcaacat gactgtgttt ttcacaccag cctgttttaa
    15001 ttgaaccaat gcgtctacgg catcttcttt caaggtatct gcaataacaa gataaccact
    15061 gtaactttga tcgatcgcta gataaagcaa tgtaccgatt tcttgacttg aaggaaaaga
    15121 gatttcaaat ctttccatca atttgtgatt acctactaat acttgtttgc catcaacttc
    15181 agctaagatc ccttcacctg caaattcctg gatattagct gttggtaaaa gcggacctgc
    15241 gtagccttct aaaacagatt gagcaatcgg atgtgtagaa aactgttctg cacttgcaac
    15301 atattgcata aatttctttg gatcaattcc tgttggatcg atcgtctgga tcgaaaagtc
    15361 acctttcgtt aatgtgccag ttttatcgaa taccactgtc tcagtttgtg caagcaactc
    15421 caagtaattt gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa
    15481 gaaactaagt ggcacggaaa tcactaatgc acatggacat gagatcacta aaaatgtcaa
    15541 tgctcgatag atccattcag aaaacgccgc tctagaaaca acaagtggag gtaaaacagc
    15601 taataaaacg gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa
    15661 attttcagct ggtgcttttt tactgctggc attttctact aattctaata atttgctgat
    15721 agtagaattt tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa
    15781 tccgcttaat actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc
    15841 tgacgtatcg actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc
    15901 aggcttcaca agtacaaact gaccgatcag cacgtcttca gggttcacgg tcatcaatcc
    15961 ttccgccgtc tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt
    16021 tcttgaatga ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac
    16081 tgctacggct tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag
    16141 aaaattctca tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc
    16201 atatcctatc agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc
    16261 aaataatagc aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct
    16321 ctttttttca tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc
    16381 tggttctaat tccttcacta ctttttttgt ttcttgttca atcgtagcca aatgatcttc
    16441 ctctgcgtca atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt
    16501 gattttctga actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa
    16561 tctgtaactt ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact
    16621 aattataaat gaatggttat tcatatgtca aggtgtccga aagaaaaaat gaaaaacatc
    16681 tgcatcggaa aatttcttcc atacagatgt tttggcttat tctttctttt tctcttcgat
    16741 cattgccact gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact
    16801 aagtttgaag cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata
    16861 tcttttttca atccctgcaa aatgtccttc ccagatatac gaagctccac tacgctttca
    16921 aaaatataga aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca
    16981 tataaataaa agacgtaaaa gtcacataga agaacttata caggaaaacg catagagtca
    17041 ggtgaaatac atgtatccca cctggctcta tgctttatct aacctttatt attctctttt
    17101 atacaatttc tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg
    17161 caaaaggtat ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag
    17221 caattggtag gcctgaaacg atcagccaga tgatattaac gatcaatgac atgccagact
    17281 ggtgataaat tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt
    17341 gcataccgat tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc
    17401 taagaccacc gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg
    17461 tatttctcct tctattactg tttttgatta aaactgggca aaaattgatt ttattcttct
    17521 ttttttagct tccagcatca agtattctgc aaattttttt tggatttctt ccgtattttc
    17581 ttttgttact aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc
    17641 tgactgttgc acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac
    17701 gagcaatagt ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc
    17761 tggatgatca gtaatcagct gattgatttg aggtaaatct tcttttactg cccaccttat
    17821 ttccatcatt tttcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg
    17881 acaattgcat ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc
    17941 ccatcttctc ttgtatattt cgaaaaacca taagcaatcc ctaaagcatt gaatatacca
    18001 tctaaaggaa ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta
    18061 gggtctaagt ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc
    18121 atcaagatga tgttgcagat ttccgctgtt tgaagttcat ttagtaattt tcgataaacg
    18181 gattcaaatt gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc
    18241 caggtatcat tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct
    18301 tcttgccagc gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat
    18361 tcaaattcca tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct
    18421 ttacctagat cagttgcttc tgctcggtat cttcctgcgt ctgtaatgct atcaccgata
    18481 aataatatac gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt
    18541 ccttatatta tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga
    18601 caccagccta gttccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg
    18661 ctattttttt cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga
    18721 tcgatcacac ccaagcttcc gtcaatcaga ccactgatga cgccatcgaa acatatattt
    18781 ataggctcat ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt
    18841 gggatagatt ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca
    18901 tatggattat tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt
    18961 tgttttcagg tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt
    19021 ccatttgaat taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg
    19081 tgaatcaaat agcaatttct tttgctttct tctcttgtct gctggaaact tccattatcc
    19141 cgtatttaaa aaaaagaact gcgagagttc gcagttcttt tttttaaata aacgttgctc
    19201 tccggccaca agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt
    19261 gtgactcgga gctgaacggc aggcgcacaa cctcttatgg ataaattcgg ttcaacaaac
    19321 gtgggaatgg aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt
    19381 caagacctag accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc
    19441 atgaatattc tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt
    19501 ccgttgcccg ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg
    19561 cacaaattac cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt
    19621 ttggatagtt taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag
    19681 gtgaaccaaa gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga
    19741 ttgcatcatc gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt
    19801 tgtctcgtcc tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga
    19861 atgctacata ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg
    19921 gttcgatcat ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag
    19981 tcggaccgaa tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc
    20041 cactttgaga taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc
    20101 cttctggtgc agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa
    20161 agaattcata cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg
    20221 aagaacgcag ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg
    20281 tgatcggata atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac
    20341 caaattttga acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt
    20401 gtagattttt agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt
    20461 ggaaaaaggc tgttccgtct cttaattgta aaaatgtgat ttttccgctt gatcgcttat
    20521 ttgcgaccca agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt
    20581 gaatttgttc cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt
    20641 gacttttact ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat
    20701 cagtagcata actcatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg
    20761 ctacccctgt ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca
    20821 tgcgtagcgt ttcttttaca ttaggaaaaa gatagaaagc tccttgcggc ttttcaagag
    20881 aaacacctgg cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt
    20941 tccgcatttc ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac
    21001 tgactgctgt cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct
    21061 cagaattccc caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc
    21121 cattgatgat gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact
    21181 cattgccatt ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt
    21241 ttactgccca ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat
    21301 tcgatggaga attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt
    21361 caacagtaac tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg
    21421 ccagttttac ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt
    21481 gcgggtcaag gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga
    21541 ctgtttgtga aggttcatag acaagtccat agtttttctt cagataatca acgattgcct
    21601 gtcgaagctc tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg
    21661 cttcgattgc tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca
    21721 aactcagcac atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag
    21781 tgacagacgg ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac
    21841 cttttaaatt cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata
    21901 attcagtttc ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat
    21961 ccctaaagca gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc
    22021 ttcgttttca gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg
    22081 aatgataacg gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt
    22141 ctttcgagta aaccaataaa aatgatcggc ttcctcaaga tttgcatatt tctttgcgat
    22201 ttctactgct tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga
    22261 gctgatcacg atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt
    22321 tttttccttt gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca
    22381 ttatagcaga ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct
    22441 ttaagaattc attgatgtcc tgatagattt cctccatagc tagctctttg atcggtactt
    22501 ttttcggtaa tgcatggatg atccgtgcac catattttgc agtgactagt cttcgatcca
    22561 aaataagcaa tacgccacga tctgtctccg tccttatcaa gcgacctaat gcttggcgta
    22621 acttcaaagc tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta
    22681 aataagcgtt cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga
    22741 ctacaacgat ctgcaaagca tccccaggca aatctacacc ttcccaaaag ctgtcagcgc
    22801 caaacaatac gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccgc
    22861 caaacccttg agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat
    22921 agaccttctg caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt
    22981 cgatcagacc tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat
    23041 ggacggcagg tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag
    23101 aggcaatcac tttaagaggt gctgcttcaa tgccaagccg cttagagaga tagtgtcgat
    23161 tagggccgat cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa
    23221 cagtcgacgg caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg
    23281 tgttcccagg aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca
    23341 tcagcaagta ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc
    23401 gcatctggtt tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta
    23461 gctgctggat ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg
    23521 gcagctgatc caacatctct tttgtcatca tgacttcttc tggatactgt ccgttaggtt
    23581 gcttgccgct cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg
    23641 cagaaagttc ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat
    23701 agctttgtat tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga
    23761 aatagttaat acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta
    23821 cttcatcgat caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt
    23881 gtattaaaaa cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga
    23941 aacgaacaaa gtcttgttcg taaaatgact gatttcttgc taaaaatcca atacctctgt
    24001 gcgcgatatc tgcaaagaat gaatggttca aatttgttaa atgtaattca tccaaatcac
    24061 cggtcttcgt ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt
    24121 gttctggcgg ttccacaagt gtgcctttaa aacgctgtaa atcaatatag tggcgatagc
    24181 ttttgataat tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt
    24241 tttctatgat ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag
    24301 gtgtagccaa ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg
    24361 cttcgatcat caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca
    24421 gtctgttctg ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt
    24481 tttttcttgg atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt
    24541 tctgcaaagc tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt
    24601 tcatttcttc aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta
    24661 atcgcgcaat ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca
    24721 aaagaagttg acccgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa
    24781 atccaaggct ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt
    24841 gtgcaagttc tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga
    24901 gttcatggtt caaaaaatta taatcaaaat aaatattatg cgcgacaaat atggtgtctg
    24961 ccaaaagatt gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt
    25021 gattcgtgat ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat
    25081 cgatcgaaaa acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga
    25141 tccgatcttc ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg
    25201 gtttcaaaat atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag
    25261 tgcagaaaaa tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa
    25321 atgtctgggc gtttattttt tagtataggt taattaagct ttagtaacgt ttgttgcttg
    25381 aggtccgcgt tggccttctt caacatcgaa agttactgct tggccttctt ctaaagtttt
    25441 gaatccgtcg ccttggatag ctgagaaatg aacgaataca tcgtttccgt tttctgcagt
    25501 gatgaatcca aatccttttt ctgagttaaa ccattttact gtacctgttt ccataataaa
    25561 aatcctcctt gtgtatcaac acatattttg caattacttg aatggtgtat atctggaaga
    25621 tgtttatttg aaacaagcta ccacagatta ccgaacaaaa actactccta tagtatatca
    25681 taccgtttac aaaaaagcta gagtttttcg acttttttta ttaaaaaaag ttttctcagc
    25741 gattacataa agtgaaaaac cgaacttttt ttaaaataaa cgacatattg ttcagttggt
    25801 gtttttttgc tctgatgttc ttctttaaac taaaaaaatt attctttctg actacttatc
    25861 ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg
    25921 tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac gatcaacgag
    25981 attagttttt cttttttgct catatctata cctcataaat aacaggatga tgtccatcat
    26041 atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac
    26101 tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt
    26161 cttctgtttc aggaatcgca gaatcaagat ctgttgtctg tacataaaca tttttgtaca
    26221 gacgtgtgcc tgtaatagga gaccgctgtg tttcttcagc aaaaactggc tgacttgtta
    26281 aacttaggga tagaaacagc aaacaccata acagtctttt tttcataaag acctccttat
    26341 cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc
    26401 ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc
    26461 tttttt
//
LOCUS       DAFGZE010000035        26393 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.035, whole genome shotgun sequence.
ACCESSION   DAFGZE010000035 DAFGZE010000000
VERSION     DAFGZE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.035"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(104..1336)
                     /locus_tag="LTU54_001617"
     CDS             complement(104..1336)
                     /locus_tag="LTU54_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBL8296333.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1591..2160
                     /locus_tag="LTU54_001618"
     CDS             1591..2160
                     /locus_tag="LTU54_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8296334.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2338..2778)
                     /locus_tag="LTU54_001619"
     CDS             complement(2338..2778)
                     /locus_tag="LTU54_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBL8296335.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2936..3700
                     /gene="map"
                     /locus_tag="LTU54_001620"
     CDS             2936..3700
                     /gene="map"
                     /locus_tag="LTU54_001620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBL8296336.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3732..4655
                     /locus_tag="LTU54_001621"
     CDS             3732..4655
                     /locus_tag="LTU54_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBL8296337.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4731..5870
                     /locus_tag="LTU54_001622"
     CDS             4731..5870
                     /locus_tag="LTU54_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HBL8296338.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5863..6663
                     /locus_tag="LTU54_001623"
     CDS             5863..6663
                     /locus_tag="LTU54_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL8296339.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6663..7490
                     /locus_tag="LTU54_001624"
     CDS             6663..7490
                     /locus_tag="LTU54_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBL8296340.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7468..8202
                     /locus_tag="LTU54_001625"
     CDS             7468..8202
                     /locus_tag="LTU54_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL8296341.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8302..9168
                     /gene="rfbA"
                     /locus_tag="LTU54_001626"
     CDS             8302..9168
                     /gene="rfbA"
                     /locus_tag="LTU54_001626"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBL8296342.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9182..9754
                     /gene="rfbC"
                     /locus_tag="LTU54_001627"
     CDS             9182..9754
                     /gene="rfbC"
                     /locus_tag="LTU54_001627"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBL8296343.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9776..10804
                     /gene="rfbB"
                     /locus_tag="LTU54_001628"
     CDS             9776..10804
                     /gene="rfbB"
                     /locus_tag="LTU54_001628"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBL8296344.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10902..11753
                     /gene="rfbD"
                     /locus_tag="LTU54_001629"
     CDS             10902..11753
                     /gene="rfbD"
                     /locus_tag="LTU54_001629"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBL8296345.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11787..13820
                     /locus_tag="LTU54_001630"
     CDS             11787..13820
                     /locus_tag="LTU54_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296346.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13864..15143
                     /locus_tag="LTU54_001631"
                     /pseudo
     CDS             13864..15143
                     /locus_tag="LTU54_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
     gene            15353..16159
                     /locus_tag="LTU54_001632"
     CDS             15353..16159
                     /locus_tag="LTU54_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296347.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16171..17391
                     /locus_tag="LTU54_001633"
     CDS             16171..17391
                     /locus_tag="LTU54_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296348.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17381..18967
                     /locus_tag="LTU54_001634"
     CDS             17381..18967
                     /locus_tag="LTU54_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL8296349.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19006..21144
                     /locus_tag="LTU54_001635"
     CDS             19006..21144
                     /locus_tag="LTU54_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL8296350.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21512..22495)
                     /locus_tag="LTU54_001636"
     CDS             complement(21512..22495)
                     /locus_tag="LTU54_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBL8296351.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22873..24264
                     /locus_tag="LTU54_001637"
     CDS             22873..24264
                     /locus_tag="LTU54_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL8296352.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIETSGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24280..25320
                     /locus_tag="LTU54_001638"
     CDS             24280..25320
                     /locus_tag="LTU54_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL8296353.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25339..>26393
                     /locus_tag="LTU54_001639"
     CDS             25339..>26393
                     /locus_tag="LTU54_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HBL8296354.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTE"
BASE COUNT         8437 a         4418 c         5373 g         8165 t
ORIGIN      
        1 aaaaaacggt ggggttgttt agcgaaataa aggatctaag ccaatagctt agatccttta
       61 ttctatgctt tatattattt tttgcatgtg tgcgaaagta tactcaagcc caatcgccat
      121 ttcggaaaat cggtactgta gagccatctt cacggattcc gtcgatatcc atcttgtcag
      181 atcctaccat gaaatctacg tgggtttgac ttctattcaa tccagcttct gctaattctt
      241 catcggacat ttctgtaccg cctttcacac tgaaggcata agcagaacct agtgctaagt
      301 gattcgatgc gttttcatca aataatgtat tgaaaaagat gatgccagat tgagaaatag
      361 gtgaagggtc aggtacaagt gcgacttctc ctaaacgacg cgcaccttca tctgtatcaa
      421 gcagttttgc taagacatct tctccttgtt cggcagaaaa atcaacaact tttccatctt
      481 taaaagtaaa tttcataccc gagatgatgg tgccagcata actcagtggc tttgtacttg
      541 aaatgtaacc atctactcga tgactgtcag gtgctgtgaa cacttcttcc gtcggcatat
      601 ttgccatgaa tttttctcca cgagcattat agcttccagc gccttcccaa agatggtttt
      661 taggcaatcc gataatgata tctgtaccgg gagcagtata gtgaagggca gaaaattgtt
      721 ctcgattcaa ttcttctgct ttttttgcta atttttcatc atgcttcttc catgctaaga
      781 cagggtcttc ttcatagaca cgagtcgttt tgaaaatctg atcccataat gcctcaactt
      841 gtttttcttc tggcagttct gggaagactt ttgctgccca ttgtttacca gccgcagcca
      901 cgacagtcca actgacttta tttgcttgag ttgccttgcg taaattcatt agtgctttgc
      961 cattcgctga ttgataagac gctactcgat ggctatctac accagcaaat gcatcgggat
     1021 cggcagaaac aacgctgatc ctgcttgctc ctttttcaag ccattcatcc gcttgatcga
     1081 ttttagattg tggtacattc tcaatacgat ccgttgcagc atgtaaaaga aattctcttt
     1141 ggatctggtc gtcagtccat tgaacgataa cctcagcggc tcctagttta taagcttctt
     1201 gtgtgatcaa tcgagcaagg ggtgcctgtt ccacgctgat ctgtaaaaca actgtgtgtc
     1261 ctttttctgt ggctacgcca gtttctgcaa ttaagcgtgc atattttttt agaagttcat
     1321 taaaatcaga taacataata aaaattcctc ctcaccattt tttctggtaa ctattagcat
     1381 gataccattt aattatgatt caaacaatga ggctttaaat aaaaaaagga ggttattttt
     1441 ttaaggttaa gtttcatgat atatgttact acttgctatt tcagcttgtg aagaaaatca
     1501 aaaactaatt ttgcatatca aaaaaaacat gttacaatga aattaatcaa tggtggaagc
     1561 gttataaaaa aattaaggag attaaaaaat atggcaagaa aaaagacgat tactagagaa
     1621 cagattttgg cagctgctta tgaagtagct gccaccgatg gatttacacg atttactgcg
     1681 agaaacatcg cgaacaaaat gaattgttcc acgcaaccga tttatcttga gtttaaaaat
     1741 atggatgact tgagacaagc tctttttgag cagatctatg atcatttaca atatgaagtg
     1801 ttcactgttg agcatacagg gaatacgatt atcgatcttg cttttaatta tatccatttt
     1861 gcaagaagag agaagaagtt gtatcgttca ctgtacttag aagaatatgg tggaggaaaa
     1921 gagatgcaga atttctctta tacttacttt gtgaatacag tgaaacgcga tccagcttat
     1981 gtgaatcttt cagaagagca gatcgaatct ttgtataatg ggacatggat catagctact
     2041 gggatcgcag ctttgatgtc ttcaagcatc atccatccaa ctgatgaaca gattgaaaag
     2101 atgatccaag acagtataga tgctatcttg aaattcaaag aaccaatcga aatcgattga
     2161 atatactcaa aagttccaca aatcagtagg taacaagaga tgtgtcaaaa taaggaaaag
     2221 ttcgtttttc ttacagcgta tgcattttta actcccgaag aactcttcca tgaatgcaat
     2281 gaataaaaga gccatacaga ttgaataaat ctggatggct cttttattgc aagtatatta
     2341 aatttttgcg actagttctt tagcgaaagc ttctaaattt tcgatatctt cttcttcagc
     2401 attcagatcc actttaacat tttcggctcc ttttgatgcg cctgtttttg caaaagcagc
     2461 atcgaaatca tccaccgatt tgcagaattc atcgtaaaaa gtatcaccag atccgcagac
     2521 accgtaaatt tttcccaata gatccaattc ttgcaagtct tcataaaagt ctacgatctc
     2581 atcaggcaga tcgccatcat cgtatgtata agtagctact acgcaaatat ctgcctcctc
     2641 gaattcatct gcatctactt gggtacattc gttgatttcg acttcgatat ccagattttc
     2701 aaatgcttct gcaacaatat cggcgatttc ttcagtgttt cctgtcatgc tagcatatac
     2761 gatttttgct aaagccatga tgtgcctcct cgtttataat tatttaacaa ttatgaatta
     2821 taccaaaaca tcattgaaaa gaaaataaaa cctagagaca cctggggaat gtcatttggc
     2881 taaatgtatg ccgctatgtt aaaatgagag ggatttgtaa aaaggagacg aaaatatgat
     2941 tactttaaaa tcagcacgtg aaattgaaat gatggcagaa tctggagctt tattagcaga
     3001 tgtacataaa aatctgcgtg attttataaa accaggaatc acaagttggg atattgaggt
     3061 atttgtcaga aactatatcg agagtcatgg cggaattgct gcgcaaattg gctttgaagg
     3121 gtatgaatat gcgacttgta tcagtataaa tgatgaaatt tgtcatggat tccctcgtaa
     3181 aaagcctttg aaaaacggtg atttggtaaa agtcgatatg tgtatcgatt tgaaaggcgg
     3241 catttctgat tcttgttggg cctatgtagt tggagaatct acacctgaga tcgaccgatt
     3301 gatggaagta acgaagaaag ctttgtacat cggtattgag caagcacaag tagggaatcg
     3361 gattggtgac atcggtcatg ctatccaaac ttatgtagaa ggcgaaaatc tagcgatcgt
     3421 tcgtgatttt attggtcatg gcgttggtcc tacgattcat gaagaaccag cagttcctca
     3481 ttacggagaa gctggaaaag ggttgcggtt gaaagaaggc atggttatca cgatcgaacc
     3541 tatggttaat accggaactt ggaaaatgaa aatggatcct aacggctgga ctgcgtatac
     3601 aagagacggc ggactttctt gccaatatga acatacattg gctattacta aagatggtcc
     3661 aagaattttg acatcacaag gagaagaagg cacttattga tctatccagt caataggtac
     3721 gggagaggta catgaaagtt tggaacaaaa taaaacaaaa tgaaaatctg atgcgcttta
     3781 ttgagacgac tcaaaagcgc atcactgatt cagaaatgag tacgacctct gtcgtcgttg
     3841 cttattattt actcttgtct ttatttccat tgattattgc ttttggtaat attttaccgt
     3901 ttctccacat cgatcaagag actgtactga cctatttacg agaagtgatt cctgagaccg
     3961 tttaccagtt tatcggtcct gcaatcaaag accttttgac acaaagttca ggaggcttgt
     4021 tatccgtttc tgcattagcg gctttatggt ctgcaagtca aagtatcaat gctttgcaaa
     4081 ttgcgatgaa taaagcgtat ggcgtagaaa atagaaaaaa ttttttgatc gtccgtttct
     4141 tctcactaat tgtcattgtc ttgttcatga ttgctattag tggggtaact atcgttcttg
     4201 ggttgggaca gctgattttg gatgctattc agccaatcgt acaaataccc gtgaacttta
     4261 tcgatcagtt ccaaactttg aagtggccaa tcacattggt agctttattt gtcatcatgt
     4321 tcttgattta tttaattgtc cctaatgctc agttgaagtt gaagacgatc attccgggca
     4381 cgatcttcgc tactgtcggc tggatgctgt tatcacaagt gtttagtatc tatgctaggt
     4441 attttgcaac gaggatcagc ggttatcaga tcataggaag ttttattgta ctcatgcttt
     4501 ggcttaattt tgctgctacg atcatcattt tgggcggaat catcaatgca gttgttcaag
     4561 aatacgtgac gggtcatcaa atcaaaaaac gtcgagatcc aagccgaaaa tggttcaata
     4621 aattgaaaac taaattttct cataaaaaaa agtagaaaca agagttctta ttgtaaattt
     4681 atatcagtaa tttataataa ttatgactct taattaaagt aggtgcccat atgcttgttt
     4741 cttttagtat ggttataaga ttttttctga caattttatt atcactgata ttaacacctg
     4801 tattcaaaat catttcagta caaacaggta tggtcgacaa accaaatgaa agaagaataa
     4861 ataaagttcc aatgccttca gcgggcggac tgcctatctt tatttcattt gttatatcga
     4921 ctttattctt attccgtaaa attatccctc aatcgtatat tgtaccgatc ttgattgctt
     4981 ctttagtaat catagtcaca ggagtgctcg atgataaata tgagctgaca cctaaacaaa
     5041 aaagtatagg gatcttgatt gcttctctta ttatttattt tgtagcagat atacgtattg
     5101 attccttcac gcttcctttt attggatata tccaactagg ctggcttagt tttcccgtaa
     5161 cgattttttg gatcttcggt atcacgaatg cggtcaatct gattgacggt ttggatgggc
     5221 tggcggcagg gatttcattg atcggtttaa ttacgatagg aatcattgga tattttttcc
     5281 tccatgcttc tacagtgtat attccagttg ttattttctg tcttgtagca agtattatcg
     5341 gtttttttcc ttacaatttt tatccggcaa agatttattt aggcgataca ggagctctat
     5401 tcctagggtt tatgatggcc gtcctttcac tacaagggct gaagaacgtg acttttgttt
     5461 catcgatttc tttgctgatc gtcatgggag tgccggtaac agacacgttt tttgctatca
     5521 ttaggagaaa agcaaaccgt gtttcttttt ctacagcaga taaaaaacat cttcatcacc
     5581 gactaatcag tttaggcttt actcataaag gtgctgtcct gacgatttat gcgatggctt
     5641 tgatgttttc tttcacagct atggtcatga attatactgg aagaatcgga acgatcatat
     5701 tgattattgc catgttgttt gctgcaattt tattatttga attgattggt ctgatcaatg
     5761 agaaacatca gtttatttta aaaacattgc gatttttagg gaataaagag taccgtacgc
     5821 aagtaaaaca gaaatatttg aagaaatgga accgtcatat agatgaataa caaattaaac
     5881 cagccgatcc ttattagtat tgtgacggca aacagtgata aaatttttcg gacactagat
     5941 aattttattt caactgtaaa agatgaaaca gcagtaaaaa tccgaatttt tgacaacaat
     6001 tcacttcccg agtacaaaaa aagactaaca gagtatagga aatatcccta catcgatatc
     6061 tattttcatg atgaaaattc cggatttggt tatggacata atcacaatct gatagctagc
     6121 gattctcgct atggcattat ttgtaaccca gatattttag tggatgaaga gacagttaca
     6181 gcattggtct tgttattgaa agaacatccg gaatgtgcga tgttagcacc taagattctt
     6241 aatgaagatg gaacaactca gcacctcatc cgtgagcagc tggctgtttt tgattacgtt
     6301 ttgcgctttg tgcctttcaa atgggtcaaa aaaaagtttg acaaacgact ggctgtgttt
     6361 gaatgccgag gattgcctac tgatgctaat agttatgtac ggatgatctc aggtagtttt
     6421 atggtagctg atattgataa atttaaagag ataaaggggt ttgatgagcg tttcttcatg
     6481 tactttgaag acaacgactt gtgtttgagt tttgaaaaag caggttacaa gttattgtac
     6541 acaccctttt atgcagttat ccatttttac gggaagggtg cgcatcgaaa tgcaaaactg
     6601 tttcgcgtgt ttttgacttc tatgatgaaa tttttcaata agtgggggtg gcgttttttc
     6661 tgatgaaacc agaaatatta gtagtcgttg tgctttatca gcaactattc agtcaatctc
     6721 cagcttatgg accattaagc aaagcactta gcgaaaagca aattcagctt attatttacg
     6781 ataatagtcc tcaaaggcag caaaatgagc tgtttgaaaa agaaaatatc gtttatcatc
     6841 atgatccgca aaatcctggt ttagcggtgg cttataattt tgcgttgtct caagcagatg
     6901 aaggaacacg ttgtctcgtc actctggatc aggatacgag gctagtcgac gattattttg
     6961 aactattgcg taaagtaacc tttacagatg aatgtgttgc tgcagttccg atgatttttt
     7021 ctgggagccg acaagtttct cccctatatt cagatcgtta tatcaatcgt acagctcaag
     7081 cagtagaagt tgaaggccca acagctgaac gaatcatggc aatcaattca ggtgttgcct
     7141 ggtctgtcaa atttctaaaa aagattggcg ggtttaatcc tgctttttct ttagattttt
     7201 tggatcattg gctgttttgg aaagtggatc agttggaaaa aactgtggag atccttcctg
     7261 tacgaatgga acatgatctt tcggttttag actatgaaaa agtttcaagt aatcgatacc
     7321 gttctatttt acaagcagaa aatcgatttt atcatgaata tgatcaaagg catttatcca
     7381 gtcaccgacg acaattattg ttaagaacag ctaaacagtt cttgaccgtc aaaaaccggc
     7441 atatctggcg gatgactttg cgctcatatg ttaagaattg gaaggtgtga attcgtgcgt
     7501 tcggtttgta tagctacctt taatggaggc agatatttaa gagagcagtt agatagtatt
     7561 ttatctcagc tttcagcaga agatgaagta gtcatttctg atgatgggtc agtagatgat
     7621 acatgggata tattaactga atacgcagaa aaagataagc gaatccagct tttttcgggg
     7681 cctcgtcaag gattgatcgc taattttggt tatgcgatcc agcagacgaa aggagatcta
     7741 atctttttgg cagaccaaga tgatgtttgg ttggagacaa aagtgaaaaa gatcactgac
     7801 tattttgagc agaatccgga tcagctagta gtagtttctg atttaatcat tgtggacagc
     7861 gagctgcaag agatccatga ttcgtatttt tcgtttcgaa attccaaaga aggatggctt
     7921 aataatgtga taagaagtca ctatattggt gctggtatgg ctttccggtc ttctttaaaa
     7981 cccgatattt tgccgatccc gtcagaagtg ccgatgcatg atatgtggat tggtttgctg
     8041 ggaggaaaga agactgggtt cctaagagaa ccgttaactc tgtaccgaag acatgattta
     8101 aacgcgagtg agattgaaac acattctaaa ttacggcaaa aaatttcttg gcgtgttcat
     8161 ctgattacgg cgttgatcaa acgaaaatgg tttaaacgtt gataaaagat gtttaaatcc
     8221 ttaatatttc catgacaaat ataacaattg atgagataat aacaaagtga aatttgaaat
     8281 caaaaaggag agcagatatc catgaaggga atcattcttg ctgggggcag cggaacacgt
     8341 ctgtaccctt taacaaaggc aacatcgaaa caattgatgc cgatttatga caaaccaatg
     8401 atttattatc caatgtctac tctgatgttg gcgggaatca aagaaattct gattatttca
     8461 acgccacaag atacaccacg tttcaaggaa ttatttggaa acggaaatga tttaggactg
     8521 cagatcgaat atgcagtaca agaaagtcca gatggattgg cacaagcgtt tatcattgga
     8581 gaagaattta ttggagatga tagtgtctgc ttggtattag gggacaatat ttactatggt
     8641 ggcggtctgt ctaaaatgtt gcaacgagca gcttcaaaag atacaggcgc gacagtattt
     8701 ggctatcatg tcaatgatcc agaacgcttt ggtgtagtag aatttgatga gaatatgcaa
     8761 gctttgtcga ttgaagaaaa gcctgctcag ccaaaatcaa attacgctgt aactggcctt
     8821 tatttttatg acaatgaggt aatttctatc gctaaaggta tcaaaccttc agaacgtgga
     8881 gaactagaga ttacggatgt caataaagca tatttggata aaggaaaact atccgtagaa
     8941 cttatgggac gcggatttgc ttggcttgat acaggaacac atgaatcatt gcttgaagca
     9001 tctaccttta tcgaaacaat tgaaaaacgt cagaacttaa aagtggcttg tttggaagaa
     9061 attgcttacc gtatgggcta catcgataag gaacaattgc tggcccttgc acagccattg
     9121 aaaaaaaatc aatacggtca atatttattg aatttggccg ctgaataagg agtccttaca
     9181 gatgaaagta acagatacaa aattaacaga tgtcaaaatc gtagaaatgg acgtctttgg
     9241 cgatcatcgc ggtttcttca cagaaagtta ttcaaaagca aaatttgcag aacatggatt
     9301 gaaatatgac tttgttcaag acaaccattc actatcagct gaagctggtg tccttcgggg
     9361 gcttcatttc caaaaaggag aagctgcaca gacaaaattg attcgggtag tcactggagc
     9421 agttttagat gtagtagtgg atatccgcaa aggtagcccg acttatggtc aatgggacgg
     9481 atttatctta tctgaacata atcatcgaca gttgcttgta cctaaaggat atgcccatgg
     9541 attcgttact ttgacaccaa atgtgaactt cctttataag tgtgataatt actataatgc
     9601 agaagcagat gggggcatcg catttgatga tccagattta gcgattgatt ggccaattga
     9661 tccatcaaga gcgattatgt cggaaaaaga tcaccgtcat ccgacattaa aagaatttga
     9721 agcagaaaat ccatttgttt acggagaaat ctaagaatta aggagaaatt ttcttatgaa
     9781 aaacatcatt gttaccggag gtgccggatt catcggctct aatttcgttc attacgtggt
     9841 aaacaatcat ccagaagtgc atgtcactgt tttggataaa ctgacttatg caggaaacaa
     9901 agagaacttg gctggacttc ctagcgatcg cgtagaattg gtcgtaggag atgttgcaga
     9961 tgcagaatta gtggaccgtc tagtaaaaga agcggacgca gttgttcatt acgcggcaga
    10021 atcccataac gataattcat taaaagatcc tttcccattt gtacaaacga atatcattgg
    10081 tacgtataca cttttagaag cttgccgcaa gtatgatgta cgctatcatc acgtatctac
    10141 tgacgaggtt tacggcgatc ttccattacg tgaagacctg ccaggacatg gggaaggcga
    10201 aggagaaaaa ttcactgcgg aaacaccata taatccttca agtccatatt cttctacaaa
    10261 agctggttca gatctgttag taaaagcatg ggtgcgttct tttggcttac gtgcaaccat
    10321 ttcaaactgt tcaaacaact atggtcctta tcagcatatt gaaaaattta ttcctcgcca
    10381 agtaacgaat atcttaagcg gtattcgtcc aaaattatat ggcgaaggaa agaatgttcg
    10441 tgactggatc catacgaatg atcattcttc agcagtgtgg ctgattttga cgaaaggcag
    10501 aattggcgaa acctatctga tcggtgcaga cggtgaagaa gataataaga ccgtgatgga
    10561 attgatctta gaaatgatgg gtcaaccaaa agatgcgtac gaccatgtaa acgaccgtgc
    10621 aggacacgac ttgcgttatg caatcgattc gacaaaatta cgggaagagt taggatggaa
    10681 gcctgagttt acgaacttcc gtgatgggtt ggctgacacg atcaaatggt acgaagaaca
    10741 tgaagactgg tggaaaaaag aaaaagaggc cgttgaagca gcatatgcca aaaatggaca
    10801 ataatacaga gaaaatgtca ctgtattaat ggaaaaacac atgtgaggca ggcgctcgcc
    10861 tgtctctttt ttaaaataaa aatcaaggag cggataatca tatgttttta ttaactggag
    10921 gaaatgggca actaggtaca gaattgcgcc atttattaga tgaaaaaaat ctgcaatatg
    10981 tttcaacaga tgcaaaagac ttggatatca cagatgccga gaaaacaatg gcttacatca
    11041 cagaattaaa acctgatgtt attttccatt gtgcagctta cacagcagta gataaagcag
    11101 aagaagaagc aaaagaatta gatgagaaga tcaatgttga tggtacaaga aatgtagcac
    11161 ttgcagcaaa agctgtgaat gctcaattcg tctatatcag tacagactat gttttcgatg
    11221 gaaaaaacaa aaatgtagaa tacgaagtag acgaccaaac gaatccacta aatgaatatg
    11281 gtcgtacgaa attattaggc gaaaaagcag tagaagagat tttagaagac tattatatca
    11341 tacgaacttc ttgggttttc gggatctatg gacacaattt cgtcttcacg atgcaacgcc
    11401 tagcggaaac ccgcgacaaa ctgactgtag taaatgacca gtttgggcga ccaacttgga
    11461 cacgtacttt ggcagaattt atggtatatg tcatcgaaga aaaagcacca tttggtattt
    11521 atcatttgtc gaacgaaaat agctgttctt ggtatgaatt cgcaaaagaa atattgaaag
    11581 atacagatgt agaagttgca cctgttacgt cagaagaatt tccgcaaaaa gcaacacgtc
    11641 cgcagtattc agtcatgagc ctgaaaaaga cagaagagtt aggatttgtc ataccaactt
    11701 ggcaagaagc acttgcgcaa atgctggaag ctgccaaaaa aatgaataag taatttcctt
    11761 ttatcaaata gaatttggag catgatatgg aaaaacaaac aagaagtgca cgacgaaaag
    11821 aaaagccaaa agcgaaccag gcagattcga atttggtgct tgacctgttt ctaaaactcc
    11881 gctatcttat tggcgtttta gtcatcgtgg tcatcgttac ttttaattta aacggaagtt
    11941 cacttggtgt ttgggataaa tatgtgtcgc aaagagacga tgggaaaaaa actgatgtga
    12001 tttttggaca gaatcgggaa gtgcggtcag acgaatggct ggtgcagaca cctttttacc
    12061 tctcacaagc tgaagaggac tttccgttgg tgaacgagga ttattcatta aacggacaaa
    12121 acatgatcat tgcgtataat tctcctgtaa aggatattac ggttatcgga aaaccattca
    12181 actggggatt ctttttcctc ggaagagatc gaggactttc cttttattgg gcgatgaaat
    12241 tagtcgtcat ggtcttgctt ggattcgaag tactgatgat attaacgaag agaaaaaaag
    12301 ctctatcgct aatcggtgca tttgcgctga cattttctcc agcagtccag tggtggttca
    12361 tgcaacatgt gggcgatttg attttcttta ctttgggact gatgactgct ttttatcatt
    12421 atttctacaa tcatgataaa aaatggctac gaacgttgat gatgcttttg gtggttattt
    12481 tcggtattgg gtttatttta gtgatttacc cagcccatca agtgatgctg gcgtacttgc
    12541 tgatttttta ttttgttggc ctgcttattt actatagcaa gaaagtttca tgggatctgt
    12601 tggatgtagt gttgattgga ggggcagttt tatttattgg cggagtcatg gttcatttct
    12661 ggctaacgtc aaaagacgca ttgttagctt ctttgaatac cttatatcca ggaaaacggg
    12721 taagtactgg aggaaagtgg acgattggcg aatttttttc tttccttaca aattggaaaa
    12781 ttccttttga agatatcaac tattcgaata actctgaagt ggccctgttc tatcactttt
    12841 ttccaactgt attcctagca agtccatttg tgttatttgg caaaaaagat agcgaacaaa
    12901 agttactggg acgtattttg atgatttttt gtctgttttc tatcttttgg attaccgttg
    12961 ggttgccaaa atggctagca gaaatcactc tgctgtctta tgttcctcca ttaagagcca
    13021 tccttacttt tagttttgcg gcttgtttat tatcgatctg gtttatcgct tatatttgga
    13081 gagaagatgt tattcctatt tggtataaat tcattttgtt ggcagtaaat gcatggtctt
    13141 atttctatgc gattacgcat tcgaaaatgg ataattattt ttcggatttt gaaaccatcg
    13201 cagttgctgt gttgggaaca gtgattttag cttttgcatt atttggctgg agacgcttgt
    13261 tttacctcgc ttttgccgcc ttagttatcg tatctggctt tttcgtcaat ccagtcgtag
    13321 aaggtacagg agcgattttt gaaaaaacac tggcaaaaga aatccaaaaa atcgatcaac
    13381 aagatcctgg taaagtttgg ttgtctgaag atgaattata taacttcacc ccaacattgg
    13441 gagtgaaatc cttcaataca gtacgctttt atccagatat agctgcatgg aagaaaatcg
    13501 atccgaaaga agaatacgag aagtattata atcggtatgc tcatacaaga gcttttatcg
    13561 ctaaggaaga tacaaagttc attttagatc gtccagatat gttttcggtc acgttgaatt
    13621 ttgaagacgc agaaaaatta gggatcgact atgtgatcag taaacgacca ttaactgatt
    13681 acaaccaatt atatgaagcg cagttcacgc agctatatgg accggataaa gacggttata
    13741 tgatttttaa agtagaatat ccggattatc cagatgtacc acaaacacaa actcaggaaa
    13801 atcagctagt cgtacagtaa taggctagct gagaataatc ctgaaatgtt agtagaaagg
    13861 agagtggaga taattcaagc aatcgtacaa aaatttgtca acaaaatgag tgtcggtttc
    13921 ttgcttttgt tactgacgct agtctttttt gtttggacag cagggttagg ttttccagca
    13981 actttgttta tatataacaa tagttcatta cttatcgctt ttagtgtggc tattttgctc
    14041 attttaaatg tttataaagc aaaaggcgtc gattttcttt atctaggtgt tgcgcttgcc
    14101 cttttcattt tcttttctta ctttcgggaa ttaagaaatg agtctttatt tggagactta
    14161 ctgtttcctt tgattatttt attttgttta gtaattaaat ggatcgagtg gacaaaatgg
    14221 gatcgcggta tttatctgat tgtcttttcg gtcttcctcg gaatcacgtt tatcgtgtat
    14281 ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg
    14341 atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct ttattgatca
    14401 gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg
    14461 ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg
    14521 tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta
    14581 ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg
    14641 aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag
    14701 gagaaaaatc tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc
    14761 aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat
    14821 ttgggattgc tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt
    14881 tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta
    14941 tggaggatac attgacttca taaccatggg ttccgattat ttatatcctg ttggctatgg
    15001 ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat
    15061 atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt
    15121 ccaggatcca acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg
    15181 cagccttttt tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga
    15241 aaatcttgta tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat
    15301 ggtaaaatgg cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa
    15361 agatttgtat ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc
    15421 gatcaatgac ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat
    15481 caatccgcta tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca
    15541 aatgatcgat gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt
    15601 cttcttctca gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt
    15661 aaagaaagtc gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta
    15721 cacccattta ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc
    15781 ttcagcaatg tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg
    15841 attgacttgg ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac
    15901 gatcgtgctg cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga
    15961 ttcaggtctt gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg
    16021 tgagtcattt ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg
    16081 ggttttcacg ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca
    16141 tttttcagat gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat
    16201 atcacaaaat cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat
    16261 ccttttaaga aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata
    16321 aaaaaaggtg agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg
    16381 aaaatcatta cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc
    16441 tcagctttgc tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc
    16501 tatttaaatg gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat
    16561 atcattgaat ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc
    16621 gggatgttcg ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt
    16681 gtggatgaag cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt
    16741 acagaaatca agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt
    16801 cgtcgttttt gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat
    16861 acgatggaag taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg
    16921 gatcggaata aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa
    16981 gcggaattgt tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta
    17041 tacgttaaag taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt
    17101 gcgctacgaa cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc
    17161 aagattcctt ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg
    17221 ttagctggtg aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg
    17281 gtatataaga caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc
    17341 gtggtcttag accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat
    17401 ggacctagat gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa
    17461 agttttagag tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca
    17521 atgtgaagtg agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc
    17581 tcaagatgga ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca
    17641 gacatttgat tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac
    17701 attgaagaaa gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa
    17761 cttggcgcat aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta
    17821 tggtctccta gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt
    17881 tgaaaaagcg ggattattca ttaatattga agattatttg tacttagctg taggtgatac
    17941 agaattaaaa tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg
    18001 accatttggt gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag
    18061 ttccattgca gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac
    18121 gaaacaagat gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac
    18181 actaagtttc cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc
    18241 tagctttatc gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa
    18301 tgctgtagtc aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt
    18361 cgttctaacg gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg
    18421 ggaattgaca ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac
    18481 aaagcagctt tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga
    18541 aaatacaaga ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga
    18601 aagtgatgaa tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa
    18661 aatccaagaa aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa
    18721 aatattaaaa atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa
    18781 gttatcagct gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa
    18841 tcaagcagaa cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga
    18901 gcaagaacgt tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga
    18961 aatgtaaata atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa
    19021 attgccgtat ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg
    19081 ggctgggcaa ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat
    19141 gtgattgctg aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat
    19201 gttaagaccc aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc
    19261 ttagacttcc aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac
    19321 ccttatgatg acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg
    19381 ataaattatg caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg
    19441 aagccaggat cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa
    19501 gagcaaagga aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct
    19561 gtctataacg ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg
    19621 tatgatcatt gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag
    19681 tgtctggaat cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat
    19741 ggacacatca gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca
    19801 ctcttagata atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta
    19861 aacgtacatc cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc
    19921 aatcgctttg atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat
    19981 tatatctctc acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt
    20041 aaaggctatg aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct
    20101 gcagatcata tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca
    20161 actgcaagta atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca
    20221 gatgccctgt ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt
    20281 tatgagattg cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag
    20341 aatggctatg aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca
    20401 aattatgaaa tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt
    20461 gcagaataca agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc
    20521 aattattctc gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc
    20581 ttgaataacg atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca
    20641 cagtttgatc gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag
    20701 catgcaggag tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat
    20761 cgaacacatt gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact
    20821 gcagcttgta tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca
    20881 ttagaagtag cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac
    20941 aatgtctatg cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa
    21001 gatactccag aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg
    21061 ccagcatata ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc
    21121 gacttttcgt tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg
    21181 cacataacgg ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa
    21241 ataagtcgag gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa
    21301 ttgcttttca aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac
    21361 cgtttattcg tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta
    21421 ttcatgctca gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg
    21481 tcaaagaatc gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt
    21541 cctctacaaa agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa
    21601 ccagattgtt cttagcgtca atataaagaa catttccact attaccaatt gctgaatata
    21661 tttcgttctc agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt
    21721 ctaaagcact ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt
    21781 ttcctttata catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg
    21841 ataaacacaa accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg
    21901 ttttaggaga cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat
    21961 tgacatggtt tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg
    22021 ttagcttact tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt
    22081 tgaattcgcc ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat
    22141 cttcacttga acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg
    22201 ttagcaagtg cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact
    22261 ctggaaaata atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa
    22321 tgccaatcag caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt
    22381 ttttataacc attccattca tctgtataaa cttctacccc atccttatat gcgacaattt
    22441 gacatatatt tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat
    22501 attacctcac tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat
    22561 tcttcacgta atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct
    22621 gaaaaatata aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct
    22681 ttaatttacc aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg
    22741 tcttgattag aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc
    22801 ttataaacta ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt
    22861 aggagtgtat atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata
    22921 ttaatggatg ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt
    22981 ggagagatcc cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg
    23041 atttttattg cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt
    23101 gacatcgtgt ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg
    23161 acttttgcgg gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc
    23221 ggtacgcttt tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt
    23281 cgagataaaa aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat
    23341 tttggttcaa agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat
    23401 gcaaacatca aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa
    23461 gaaactgaaa aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg
    23521 aatacaacat ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa
    23581 agtattattg aaacatctgg atttagaatc caacctgaat atgaactatt caaacgtttg
    23641 tttgatatca tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca
    23701 gcaattttag tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca
    23761 aaaaatcaaa aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca
    23821 aaatctggac cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt
    23881 attcgagctg tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg
    23941 tcgatcgtag gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac
    24001 ccttattact acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat
    24061 ggaaaatatg tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa
    24121 tattcattga tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa
    24181 atgtcctctc aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt
    24241 ccgaatgaaa aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc
    24301 ataatcgtac cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa
    24361 gaattggatt atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca
    24421 caggaaatca ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc
    24481 aatcaagggc ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc
    24541 ttattcttcg atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg
    24601 ctagaaaaga gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac
    24661 gagggaagaa tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac
    24721 gaagagttta taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg
    24781 aacaaatgct atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat
    24841 agttgtgagg atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg
    24901 aacccaaaag tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac
    24961 cgacctaaca agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac
    25021 gatattaata aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt
    25081 tctatttata gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata
    25141 ttgagtgatc cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa
    25201 aaaataacaa agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt
    25261 ggaagcttcg ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag
    25321 ttaatggagg aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt
    25381 ggagcagagt ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt
    25441 gattttttga tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga
    25501 ttaggaggcc gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata
    25561 gaagtgaata agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt
    25621 ggcgggggaa ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat
    25681 agccatgcaa ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt
    25741 ttaaccaaat tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt
    25801 gaaagtctgt ttcaaaacca tccctttgag atagttcata atggtattga tttagagctt
    25861 tatagtaaaa attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt
    25921 acttttgttg tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta
    25981 gagatttttt tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa
    26041 gggtcactta gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta
    26101 aaatttttag gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta
    26161 atgccttctc tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta
    26221 aaattagtct tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt
    26281 gtatcattag atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct
    26341 aataaaataa cacaagaact aaagagttat gatgtttcgt acactactga aca
//
LOCUS       DAFGZE010000036        25958 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.037, whole genome shotgun sequence.
ACCESSION   DAFGZE010000036 DAFGZE010000000
VERSION     DAFGZE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.037"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            6..305
                     /locus_tag="LTU54_001640"
     CDS             6..305
                     /locus_tag="LTU54_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296355.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="LTU54_001641"
     CDS             378..1739
                     /locus_tag="LTU54_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL8296356.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="LTU54_001642"
     CDS             1761..2021
                     /locus_tag="LTU54_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296357.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="LTU54_001643"
     CDS             2035..2787
                     /locus_tag="LTU54_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBL8296358.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="LTU54_001644"
     CDS             complement(2881..3486)
                     /locus_tag="LTU54_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296359.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="LTU54_001645"
     CDS             complement(3539..4513)
                     /locus_tag="LTU54_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HBL8296360.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="LTU54_001646"
     CDS             complement(4891..5610)
                     /locus_tag="LTU54_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8296361.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LTU54_001647"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="LTU54_001647"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBL8296362.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="LTU54_001648"
     CDS             7161..8558
                     /locus_tag="LTU54_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8296363.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="LTU54_001649"
     CDS             8639..9079
                     /locus_tag="LTU54_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL8296364.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="LTU54_001650"
     CDS             9227..9553
                     /locus_tag="LTU54_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL8296365.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="LTU54_001651"
     CDS             9554..10303
                     /locus_tag="LTU54_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296366.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="LTU54_001652"
     CDS             complement(10383..10838)
                     /locus_tag="LTU54_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL8296367.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="LTU54_001653"
     CDS             complement(11265..11540)
                     /locus_tag="LTU54_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296368.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="LTU54_001654"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="LTU54_001654"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBL8296369.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LTU54_001655"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="LTU54_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBL8296370.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="LTU54_001656"
     CDS             14770..17436
                     /locus_tag="LTU54_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL8296371.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="LTU54_001657"
     CDS             17800..18552
                     /locus_tag="LTU54_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8296372.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="LTU54_001658"
     CDS             complement(18574..19728)
                     /locus_tag="LTU54_001658"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HBL8296373.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="LTU54_001659"
     CDS             19913..22354
                     /locus_tag="LTU54_001659"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HBL8296374.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHIGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(22684..23388)
                     /locus_tag="LTU54_001660"
     CDS             complement(22684..23388)
                     /locus_tag="LTU54_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296375.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(23575..23820)
                     /locus_tag="LTU54_001661"
     CDS             complement(23575..23820)
                     /locus_tag="LTU54_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296376.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(23837..24688)
                     /locus_tag="LTU54_001662"
     CDS             complement(23837..24688)
                     /locus_tag="LTU54_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL8296377.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            24854..25858
                     /locus_tag="LTU54_001663"
     CDS             24854..25858
                     /locus_tag="LTU54_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBL8296378.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         8298 a         4537 c         5358 g         7765 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatagga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttttctctat cagaaaatat
    22441 gtttattatt agaatttttt atagttaagg ttaggcatct taagaaatca ggcaactcca
    22501 agatatcttc aggtagaaga gaagtcgcct aacattgtag gatcataggt gatttgttgg
    22561 acaggtcaag ctgatatttg agcctgatgt tagaaggaat ttctttttac ttgctgtaga
    22621 aattattata tgactaaaaa ggctgggatt ttaaatccca gccttttatg acaaatagaa
    22681 tttttatcga tcagtaatcg ctgtcgctgc aaattgttcg atcaaaggaa tcaaagtcaa
    22741 tttgactttg tatttccgtt cgagtgcttt ttcgataacg aactcatttt cagtagtcat
    22801 agaaatagtt gctaatttac gaggttcatc agcatttttt tcattaataa ctacttcatg
    22861 ttcattttgt tctgtgtaaa atttaggata ctcagcaacg gcttttgaaa aatcagcaaa
    22921 acttaaagcg gcattcgtat cagttgagcg tgtattttca gttgtgtccg cttttggttt
    22981 ttccgttttt tcttctggca cactgactgt ttcttctgta actggtgtcg caacagcttg
    23041 cgcagttggt tcatcgtttt tcaatcgttg atcattccag taagacacga tttcaataag
    23101 aagattgtca cggaatcgtt cgaaaatccc gttttctcct gaacgaattc tctttaatac
    23161 agtattgaag ttgtcatcga ttggcacgtt gatccgtgta tcttcatttg ttttcccagc
    23221 taatccgatc gttaagattt gacggttttt gggatcaagc atgacttgta acggcaaacc
    23281 ataacgtcgc aagctctctt cttctagaga acggaataat tgtcctctta caccatccaa
    23341 tgaaggacgg aattgattta ataatttttg tttctgtttt ttcttcatat gttctctcct
    23401 tcgctcatac cctttcgatt ataagaaaaa aacagccaaa tgaaaaggag taatgcaaaa
    23461 aaacttgcgt atacgcaaat tacaacagaa aaatttgcgt attttcccgt tttttgaaag
    23521 aaaaataaaa aatggaacgg attatgtata tatacataat ccgttccatt ctttctattt
    23581 tttcggtatc aaggcaaaaa caggtagttc aggtgaataa atgacttcat gttcgtgcca
    23641 gggtttgacc attagcgaat gatcgaaccg ttttttcagt tcttcgatga tttgatcatg
    23701 tgtccagttt cttgcaggaa aatgttggat tttctctgca tcgatcaaaa agacaaaatc
    23761 ggaatgaaaa gtctgggtaa cttcttccga gagagaattc aagcgttcga ttggatccat
    23821 aaaatcacct ttcgacttaa aattgggtag tatctgggtc ttttgctttg ccgactacac
    23881 catctaactg atcgatttgt ttcatttctt cttctgttaa ttcaaaatca aataattcag
    23941 tattttcttt gattctgctt ggtgtcactg acttaggaag aggaaggaat tcgtgttgca
    24001 gcgaccaacg caaagcaact tgtgcaatcg tcttgtcatg tgcttcagcg atttgtttca
    24061 tttcaggcac atcgaagatt ttaccagtgc ctaatggact atatgcttcg aggatcatat
    24121 catgtttttt tgcatactca actacagttg tctgtaattc gcctggtgca aggaaaactt
    24181 gattgaccat cggcataatt tttgctgttt ttgctagtgt atctagatgg tggggtaaga
    24241 aattgctcac accgattgct ttgattttac ctgcttcata cagttcttca aatgctcgcc
    24301 atgtatctgc atttgcttgt tcccaattat cacgaaaagc aacagggttt ggccagtgga
    24361 tcaaaaacag atctaaataa tctgtttgga gcttcttcaa tgattcttca aaagaagaca
    24421 tcacaagttc atagctgtgg tttgcattcc ataattttgt tgtcaagaat atctcttctc
    24481 taggaattcc actttcctta atcgcttggc cgacgctttc ttcattcttg tatccttggg
    24541 cagtatcgat atggcgataa cctgcagcca gcgcttcttt aacagaggaa acagcgacat
    24601 ccccatcagg tgtttgccat gtgccgaatc ctactaccgg gatttcgtaa ccatttgata
    24661 gacgataagt gcttgttaat gaattcatac tttcagctcc ttttttattc gttcaactat
    24721 aagtgtagcg catttttcga aaaaaagaat ccattttgtt tctactcttg aaatttttgt
    24781 ttaaaagaaa atgattttga ttctggtatt tttgattttt tttccgtaaa ctaagacaga
    24841 agggaatgag aaaatggata tctatttaaa aaaagcaatt ctgcatatta ttgacagaga
    24901 aactggttct ccagtctttt cacaaaaaga gctggatctg acaaaagaat atattcgtga
    24961 ttttcttcaa aagaagatcc aaaaaatctc ttctgctcaa acaaaaactg gacaactagc
    25021 agaagaaagc atgatcgttg aatcacttaa aatcgctcaa gaaaattttg tggaagcaag
    25081 tgaagcaatc gttcagcggt ggttcgatat ctaccaagaa agtgaagaag cacctagtgc
    25141 agatgtattt gttgtcttgt atgaattaga tacagtgatg tacttatctt tattgaaagt
    25201 caattatcgt gaggcttaca ctcactttgt tgaagcagac gaagcaggga tcgacaaccg
    25261 cttgattttg aatcgagcaa ttctaggggc gaaaacacaa aaagcagatg aagcattcgc
    25321 tgtgaatttg actgattgga cctacgaatt aattgaaaaa aaatatgagt tttctggcaa
    25381 aaaagagctt tatttttctg gaagagtgat cgaaagtgtt cctgcacctt ctttagaaga
    25441 aaatgtaaaa gtgatccaaa aagtagccaa gcatcttgga aaaaaatttg agacagaaga
    25501 atttgatatc atggctgatt tgaaagaagc tgtctatgat acgatagaag aaaaaggtcg
    25561 gttggatcat gagatgattg cggaaaaagt cttcaaggaa aacatcacgg caaaaatggc
    25621 ttttaaagaa gaagtacaag agcaaggatt tgtcccagaa gcacctcctg taaaagaagt
    25681 acaagaaatt tctgagaaaa aatttggaaa acaaaaattc aagctatcaa acgggatcga
    25741 actgatcgta ccagtagatg tatatcgcaa cccagatttg attgaatttg tgaataatcc
    25801 ggatggtacg atatccgtga tgatcaaaaa tgtggatgaa gtattgaatc gtttataaaa
    25861 atgactagaa tattaggtgt tcaagtacca gtgatcagtt aaatgaattg tgtcaagaga
    25921 aggatcggaa aagacgaata taacaaaaaa agagcgtg
//
LOCUS       DAFGZE010000037        25452 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.038, whole genome shotgun sequence.
ACCESSION   DAFGZE010000037 DAFGZE010000000
VERSION     DAFGZE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.038"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            54..2114
                     /locus_tag="LTU54_001664"
     CDS             54..2114
                     /locus_tag="LTU54_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HBL8296379.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2197..2766
                     /locus_tag="LTU54_001665"
     CDS             2197..2766
                     /locus_tag="LTU54_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296380.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(2941..3747)
                     /locus_tag="LTU54_001666"
     CDS             complement(2941..3747)
                     /locus_tag="LTU54_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296381.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3885..5399
                     /locus_tag="LTU54_001667"
     CDS             3885..5399
                     /locus_tag="LTU54_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HBL8296382.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5802..6785)
                     /locus_tag="LTU54_001668"
     CDS             complement(5802..6785)
                     /locus_tag="LTU54_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HBL8296383.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6790..7437)
                     /locus_tag="LTU54_001669"
     CDS             complement(6790..7437)
                     /locus_tag="LTU54_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8296384.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7522..8154)
                     /locus_tag="LTU54_001670"
     CDS             complement(7522..8154)
                     /locus_tag="LTU54_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL8296385.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8170..8721)
                     /gene="coaC"
                     /locus_tag="LTU54_001671"
     CDS             complement(8170..8721)
                     /gene="coaC"
                     /locus_tag="LTU54_001671"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HBL8296386.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8718..9455)
                     /locus_tag="LTU54_001672"
     CDS             complement(8718..9455)
                     /locus_tag="LTU54_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HBL8296387.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9452..10441)
                     /locus_tag="LTU54_001673"
     CDS             complement(9452..10441)
                     /locus_tag="LTU54_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL8296388.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10699..11241
                     /locus_tag="LTU54_001674"
     CDS             10699..11241
                     /locus_tag="LTU54_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8296389.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11258..12343
                     /locus_tag="LTU54_001675"
     CDS             11258..12343
                     /locus_tag="LTU54_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296390.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12355..13161
                     /locus_tag="LTU54_001676"
     CDS             12355..13161
                     /locus_tag="LTU54_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296391.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13162..13989
                     /locus_tag="LTU54_001677"
     CDS             13162..13989
                     /locus_tag="LTU54_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296392.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            13986..15059
                     /locus_tag="LTU54_001678"
     CDS             13986..15059
                     /locus_tag="LTU54_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8296393.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15487..18183
                     /locus_tag="LTU54_001679"
     CDS             15487..18183
                     /locus_tag="LTU54_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8296394.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18260..18748
                     /locus_tag="LTU54_001680"
     CDS             18260..18748
                     /locus_tag="LTU54_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8296395.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18760..19254
                     /locus_tag="LTU54_001681"
     CDS             18760..19254
                     /locus_tag="LTU54_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296396.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19275..20084
                     /locus_tag="LTU54_001682"
     CDS             19275..20084
                     /locus_tag="LTU54_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296397.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20081..20902
                     /locus_tag="LTU54_001683"
     CDS             20081..20902
                     /locus_tag="LTU54_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8296398.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            20953..23052
                     /locus_tag="LTU54_001684"
     CDS             20953..23052
                     /locus_tag="LTU54_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HBL8296399.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23049..24167
                     /locus_tag="LTU54_001685"
     CDS             23049..24167
                     /locus_tag="LTU54_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL8296400.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24206..25084
                     /locus_tag="LTU54_001686"
     CDS             24206..25084
                     /locus_tag="LTU54_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8296401.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25275..25427
                     /locus_tag="LTU54_001687"
     CDS             25275..25427
                     /locus_tag="LTU54_001687"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBL8296402.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8031 a         4436 c         5404 g         7581 t
ORIGIN      
        1 ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa taaatgtgtt
       61 caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag gaacggtcgt
      121 gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt ttatctgtag
      181 gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc gtacgtacct
      241 ctgtttctga gaccactgat tcccctatca ctattactca tactttcact gatgcaaatg
      301 gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct ccaacgataa
      361 accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga agcagtgtgc
      421 cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta aaacagatca
      481 ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca caagtttctt
      541 atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga
      601 caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga acacaaggag
      661 atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat acaggaacat
      721 ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact gcatctgctg
      781 tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac ttaagaacag
      841 gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat ggaacttcag
      901 gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg ggagaatatc
      961 ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca acagctgtag
     1021 tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata gatagtcaaa
     1081 caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt cctgatttgc
     1141 gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact tatgaacaaa
     1201 atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac agaaaaagtc
     1261 actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg aagcaatcag
     1321 gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta gaaggtcatg
     1381 atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa tcagcaacaa
     1441 attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat gaagtaccat
     1501 tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat agttatcatg
     1561 acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta ccattaaaca
     1621 aacagataga tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa aaaaaacaac
     1681 aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc ctagagttag
     1741 caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa gctcatttgg
     1801 ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa caacaatcag
     1861 aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac cagaaggaaa
     1921 cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag ccgcaagtac
     1981 cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct gctttaggag
     2041 atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag
     2101 atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct gagtcatatg
     2161 ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac gttaccttac
     2221 gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc tttagcggaa
     2281 ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga gattgagact
     2341 tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca aaactttgct
     2401 ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa
     2461 gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat
     2521 tggttagcag tgaaaccaga ttaccaagga aaagggctcg caaaagcatt gactgccaga
     2581 gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca cacacagaca
     2641 tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat tgctgaaaat
     2701 ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag taaattagat
     2761 aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa tagtcattcc
     2821 tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc atacattcaa
     2881 aaaagaaggg gcagtgacaa aagtattgtc actgcccctt ctcttgtttt ttagcatgtt
     2941 ttatcgtttg atttttttag gaataaaatt cttgatgaac gtaaattttt ggtaatcgtt
     3001 tcgactatag aggtaaccaa atacactgaa aacaactgct ccaataaaat taacaaataa
     3061 atccttcata gtatccatga ttcccacgtc taagtaacca ttttggatga ttgttttatc
     3121 tgttttacca tctgcatttt gactttcaat gattgtccga gtgatatctg gaacatgata
     3181 gacagtgttt ccgtctttac ttaactccac actattgatt tgctggatga tcgtatcttt
     3241 ttgcatatcg aattgcagta atttatctgc tgaaaattcc acaaactccc acataacacc
     3301 aactgtcatc gaaaaacaga aggaaacaac tgctagtaat aaaggagtca agttcatggt
     3361 ttttgtattt ttattgagca aataaacaag ggaaaagccg acacccccag ctaaaaatcc
     3421 gctgaatgca tgtagaaaag tatcccacat tgaaaaatag ccataaaaat cacttaactc
     3481 tcctaaaact gttgaactaa aaataaaaga taacaaaatg atttctagca tatttggaat
     3541 ctgtatcttt aatatctttt ccagaaaata agggatttca aataaaatta atgtcagtaa
     3601 taaaagcatt gtatttcccc agtgttggtt ccagacctga tccactagca taataacgac
     3661 gatgatacgt aataaaatat agattccgtt tgtgattcta cctgcagttt ttgtactgtt
     3721 cgtaaaaaag gttttcaaat gattcataaa ataactcctt catgtatatc cttaattata
     3781 ctaagaagcg tgtgtttacc aaagctatat gacttaaaga aaaacagagt tgatattgtg
     3841 aaaaaaatca cttaatatag tataaggaga aaagggggat acaaatgaaa aaattagttg
     3901 ctggtttatt aacagccgtt gcacttcttg ctatgagcgc atgcggcgca gatcagaaaa
     3961 atacagcgca gacaagttct agtacgaagg aatctgccga agtatcatca ggcgcttctg
     4021 aacaagtata tacagatcca aaagaaatga aagaatctta tgatgtcgtg attattggag
     4081 ctggtggtgc agggatggca gcagctatcg aagcaaaaga tgctgggttg aatccagtaa
     4141 ttttggaaaa gatgcctgtt gccggaggaa acacatctaa atcatcaagc ggtatgaatg
     4201 cttcagaaac aaaatttcaa aaagaagaag gaatcaaaga ctcgaatgat gccttctatg
     4261 aagaaacgct aaaaggtggt ggaggaacga atgataaaga aatgcttcga tactttgtcg
     4321 atcactctgc tgatgcggtc gattggctag atcaaaacgg tatcaaactg gacaacttaa
     4381 cgatcactgg agggatgagt gaaaagagga cacatcgtcc gtctgatggc tcggctattg
     4441 gtggatactt agtggagggg ttattgaata acgtccatga gcgggaaatc cctatttttg
     4501 tcaacgcaga tgtgacagac attaagaaaa atggggaaac ggtcaatgaa gtgactgtac
     4561 atgttcaagg agaagaacca aaagaggtca ccggaaaagc aatcgtagtt accacaggcg
     4621 gttttggtgc cagcaaagaa tttattgaaa aatatcgtcc cgatttagct gattatatta
     4681 cgacaaatca agaaggatca actggagatg gtatcaccat gattgaaaaa ttaggtggac
     4741 aaaccgttga tatggataaa atacaaattc atccaactgt gcagcaggat gagggggtct
     4801 tgattggtga ggttgttcgc ggagaaggag caatcttggt cgatcaagag gggaaaagat
     4861 ttgtcaatga aatggataca agggataaag tatctgcagc aattacagca ttgcctgaaa
     4921 aatcagctta tttgattttt gaccaaggcg tgcgtgatcg tgccacagct attgaatttt
     4981 atgaccaaaa aggatatgtc acaactggaa agacaatgga agaattagca gaaaaaatcg
     5041 atttaccaaa agaaacacta gcaaaaacag ttgatggatg gaatacggat gttgcggaga
     5101 aaaaagatgc tcaattcaac cgtgccacag ccatggaaca tgacttatcg tctccgaatt
     5161 attatgcgat caaaatcgca ccaggaatcc attacacgat gggcggtgtg aaaatcaata
     5221 cgaacactga agtattagat aaagaaaacc agccaattag ggggttatat gctgctggtg
     5281 aagtcacagg tggcttacat ggaaacaacc ggatcggtgg aaattcagtt gcggagatca
     5341 ttatttttgg gcgacaagcg ggcatacaag cagctcgatt tgcttcggaa aatgaatgaa
     5401 atagaacgta aataaaaaga ggttgtgaaa tcagcgtttt gagctaatac cgagggtcag
     5461 ctgattgaac actgtttagt gaagaggttg tgaaaaagct gccctgcatc aagtaataag
     5521 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ttggacttat taccgcagat
     5581 gcaagctttt gaacaccgtt tattcggaaa aagagcgtgg gacaaaagta aagaacactt
     5641 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agaaattcct tcggaaataa
     5701 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
     5761 taaacgcttc tgtcccagcc tcttttttga tcagcatgat tttattcagt tatcttcaaa
     5821 atgatttttc cgatgttttt gtttgcttcc atatattcat gcgcttgttt gactgcgctg
     5881 aattggaatg tacggtcaat gattggccga atcttgccat tttctagaaa aggcccagct
     5941 ttttgcataa aagtttgtgc gagctctgct ttatactgat cacttctagg tgtcaataat
     6001 gtaccaagca gttggacacg tttcccaatc aatacaccaa ggtctatctc aggcacgatg
     6061 gtcccaccta gcataccaat caagacccaa cgtccatcaa cggctataga agctaaattc
     6121 ttttcccagt aagaagcgcc gatgaaatcc aaaataacat cgactccccg accttcagtg
     6181 atttttcgta cctcttcttg aaagtcttgt tttttatagt tgatcaattc atctgcacca
     6241 agttctttac aaaaggcgag tttttcttct gatcctgcag taacgatgat tttcgcgtga
     6301 gaaagctgtc tagctaattg gatcgctgct gttcccacac cacttgctcc tgcatggatc
     6361 aagacagttt cgccttcttg taattttcca agccagtaaa gtgtttgata agcagtcaaa
     6421 aaaacttctg agattccagc agcagaaaca tagtctagcg tatcagggag aaggattgca
     6481 cgatcggcag gcatcacagc atattcggca taaccaccta gattgactaa tccgtatact
     6541 tttgtcccag gagtaaaagt ggagatatca cttttattct caactacctc tccagctact
     6601 tcgactccca aaatagggta gggaggtttc aaagagactg attctctttt cataatatct
     6661 gttcgattga cagctgctgt atggactttg atcagtaatt cgccttgttt tggtttagga
     6721 acaggcactt caatttgttc taatacatct acagaaccag gatgcttgat tgaaatggca
     6781 cgcatggatc tattcctcct ttgcccattc ttggaatgct gtattcaact ctttgctacc
     6841 ttcagaaagt actagagtcg tacgagaaat atccgtttct tccactagag cggcaagtac
     6901 agcagcaact gtagagcgag tgatcgtttt tgatgccttc tcattaggct tagttaattg
     6961 aatggaagta acttcttcat cgttagtcaa tgtgactggt tgaagaatga caaaatctaa
     7021 gttagttcgt ttcagccatt catctgcata atgtttagta atataataat ctgtcatgga
     7081 ctcaggccat tttgctggtt catctgcata aacagcactg accatcagat agcgagagac
     7141 atgcgcattt tcagctgcga tcactgtttt gattgcgcca tccaagtcta cttgcagaag
     7201 attctttcct tgagagccgg cagcaaaaat caatacatcg atgcctttga atgcttcggt
     7261 cattttttcc acggaccaag ttaaatcaaa agagacatac gaaacattct ctttttttac
     7321 taatgtttgg gaagcaacat ctctcacccc tgcaaaaatt tcatgggaac ttggagctaa
     7381 atcttttatc aggtgtcggc caatttgtcc gtttgcacct acaacaaaaa ttttcatttg
     7441 gatcatcctt tcgacgtcaa atctttccat tcaatagtaa caccaaaaga gaaaaaaatc
     7501 agagatcggc tacgatgact atcagttagt aagagaaggg tgcttgaagt atcttaacaa
     7561 gacattgcat actgctgcag tcgcaagacc tgcgacgatt ccttcaggta taccacttgt
     7621 aaaaataacc gctagaatag ctgaataaac agcatttaaa tttgttccga tgactttggc
     7681 ataatcttgc tggaataaaa agtagatcat attcatcact aaaatcgtat tggtcaatga
     7741 accggaaagt ccagctaaga ataaagaaac ggaatctgct ttttgtttca ttagtttgcg
     7801 cagaccttta taaacgaaat aaggaaccac cccaatcaag atgcgaggaa taaacgcgat
     7861 cagcaaggct ttccagcttc catgttctgt tccgatgact gggataaacg gagaaaagac
     7921 aaaagacaaa ggtgtttgga taactgtgct acggatcatg ctgatcagtc caaatgtacc
     7981 gccaagaaaa gcacctagtc ttggtcctaa aacgatcgaa gcaatgatta caggaatatg
     8041 catggttgtt gcattgattg gaccgatagg gataaaacct aaaggcgtaa cagctaataa
     8101 aatcaaaata gctaaaaaca tcgcagttaa tgtaaagttt tttgtatttt tcataagtac
     8161 tccttttttc taagcatttc tttcattaag agtcagcatg acctgttcga tgatcgtctg
     8221 aatatcagct aaggcacctt ttccaaaatc tccacaagct aaaagagatt ctcttggttc
     8281 aatttcctga tagccgacag atctcaatgt ttccagatta cgctgagtga tcgggttttg
     8341 atacatattc gtgttcatgg caggggcaat caattttggt gttcccactg gaagcgccat
     8401 tgcgactgtt gaaagaagat cgtctgcaat gccgtttgct agcttgccga taatgtttgc
     8461 tgtagcaggc gcgatgagaa acaagtcagc ttgttttgct agttcgatat gattgattac
     8521 ctgcggattt gattcttgca tgacgtctgt atggactgcg cgcttcgaca gagattgcaa
     8581 ggttaatggc gtgataaaat gtgtgctgct tttagacata atcacatcta cctgggcacc
     8641 taatttgacg aattgacttg taagatcagc gcttttgtaa gcagaaatac ttccagtaac
     8701 gcctagtaag atctttttca ttttgattcc tccagtctaa ttttctcagt aatcaatgca
     8761 gcaatctcag atttactttg agcttcttcc actgttccat ctttagataa gagatagccg
     8821 tggtgtgtct cgccatggat ttccgtcaaa tcatttgcga aaataaaatc agttttgtta
     8881 tttatcagac tattttttgc gactgccaga agagattcct ttggtacatc tactaatagt
     8941 ttgaaaccaa tgatttttgc ttttggctgc cattcacgta gataagaaat gatttttggt
     9001 gttttcttca aaacgagcac aagatgctcg gtgttagaag agatctttga aacagtggta
     9061 tctgtctgtt cattttccgc aaaccaattg tccagttctt cttgcgttga agaagtgggt
     9121 aggttttttg ccagctgttc ttctgaaaaa ctaaaagcag gagtaaaatc actgactgcc
     9181 atactgtgga taatcgcatc gtagtgttct tttttcgtta gttcttctaa ttgtaaaaat
     9241 aagtccagtg cagattcgat cgtataaatc gttatattgg agtgtctttt cggtttcagt
     9301 gcttgtttcg tagtcacgta atcgattgtg gtatccggat tggcagctaa ataatcagct
     9361 aatgcttgcc ctaatcgtcc tgtcgaatga tttgtaatag cacgtacttg atcgattttt
     9421 tcagtcgttc ctccagctgt gattagaatc ttcatttatg gtcacctaca tttactagtg
     9481 ttcttccttg gtgtgttcca gcaagtagtt tagatgctgt ttcaggcaat tgatccagag
     9541 taatctcttg gacgaccagt tcatctgcaa tctgcaaatc cgccatctgt tgccataaag
     9601 aaagacgttg atctattggt acattgacag aatcaattcc aatcaactgg atgtttcgca
     9661 gaataaaagg caagacagtc gcgtttagtt tgattccccc agcattgcca cataaagcta
     9721 ctgcaccgtt ataagagatc aatggaagaa gactgcttag ctgctcgcca cctactgtgt
     9781 cgatgacata gtcgatttgc tgttttccta aagcttttgt tgtctctggc agaaattcat
     9841 caggtcgtac gacttgcaaa gcacctagac tttttagcca tgtgacagac tcttcttttc
     9901 tggaaaaggc aatgattgac tggaatccta gtttatgtaa cagggctatc gctgtactgc
     9961 ctacaccgcc agaagcacca gtaacgacaa cttttttatc ggaggtcata ccttgacgga
    10021 ttaaagcgtt cacacacaac atagcagtaa accctgctgt accaaggatc atcgcttgac
    10081 gcagcgtcat attttttggt agaggaacta accagtctcc tggtactttt tgatattggc
    10141 tataaccgcc tggatgggtg acgccaagcc caaaaccagt agcaagtaca agatcttctt
    10201 ttgaaaaacg tggattgtta ctttggacga cagttccgga taaatcaatc cctggagtca
    10261 tcggatatgt tcgtatcacc tgaccagact cgctcattgc caatgcatct ttataattga
    10321 catcagaata ggcaattttt accaagactt cattttcagc caactcagta aatggcagat
    10381 cactgattgc tgtatggaaa acaggttcct ttattacctg aaaagatcga aaagcgttca
    10441 ttcgtgagac actcctttta aaattttctt ttatcatacc ataaagccta ctcttttatt
    10501 cttgttcttc cgtaaattta gacaaaaatt aggtgaaaaa taaagaatca aacaactaga
    10561 atttattcca aaagataaag aaatattcag caaaaaaaat tttacaaagg gttgcctttt
    10621 tcaataaatt tcgtatacta atacgagtgg tagtaaacag gaagtttact attagaaaac
    10681 ttcgattcgg aggaaattat ggaaattggt gagaagttaa gaaatttgcg catccaaaag
    10741 aatttgacac aagaagaact aggcgaacgc acagatttgt caaaagggta tatttcccag
    10801 ttggaacgag atttaagttc accatcaatg gaaacattct tttctattct tgaagtctta
    10861 ggtgtgactc cagaagaatt ttttcatcaa gaagcagcga atctgcaagt ggtttattca
    10921 aaagaagatc atacggttta tttcgatgaa gaaaacgggt atgaattgga atggctcgtg
    10981 gctgattcga atgaaaaaga aatggagcct gtcttgctga cttttgaagg tgcaggagaa
    11041 tataagacgt ttgaaccttc attatctgaa acgtttattt ttgtattaga aggggaatta
    11101 gagttaagct taggagagac ggtctatgtg gcaaaagcag gacagtcgat ctattatcaa
    11161 gcgacaaaac aacatcaatt aaggaatcat ctcaaaaaac gaacaaaagt attgattgtt
    11221 gcaacgcaat catatttgta atataggggg taaaaacgtg gggaaaccaa tcatttcatt
    11281 tgatcaagta gtgaaacgct atgatgatga aacagtatta aaaaaagtaa gttttgagat
    11341 cgaacaagga aaattttata cactgcttgg tccttccggc tgtggtaaaa caacgatcct
    11401 acgtattata gctggattca ccgaagctac agaaggggac atctattttg aagggaaacg
    11461 gttgaatgat ttgccagcta ataagcgaca agtaaataca gtcttccaag attatgcact
    11521 ttttcctcat atgaatgttt ttgataacgt tgcttttgga ttgaagatca agaaaatgtc
    11581 caaatcagat atcgaaaaga aggtaaaaga tgctttgcgt atggtccagc ttcctggata
    11641 cgaaaaacgg gagattagtg aaatgtctgg tggtcaaaga cagcgtgtag cgattgcacg
    11701 agcaattgta aatgaaccga aagtattgct attagatgaa ccgttatcgg cgcttgattt
    11761 gaaactacgt acagatatgc agtatgaact gcgggatctg caacaacgat taggaatcac
    11821 gtttattttt gttactcatg accaagaaga agcgttggca atgagtgatg agatttttgt
    11881 catgaataaa gggaaaatcg tccaaagtgg tacacccgta gatatttatg atgagccaat
    11941 caatcatttc gttgctgatt ttgttgggga gagcaatatc gtggacggtg taatgatcga
    12001 agataatcta gttgagtttg ttggtaagca atttgaatgt gtcgatggcg ggatgcgtct
    12061 aaatgaacca gttgaagttg ttttacgccc agaggatttg acgatcacga caccagataa
    12121 aggaaaacta gtcgtaacgg tcgatactca gctgtttcgt ggtgtccatt atgaaatcat
    12181 ctgttatgat gaacagcaaa atgaatggat ggtccattca acgaaaaaag ctaaagaagg
    12241 aagcaaagta ggattggcct ttgagccaga agatatccat gtcatgcgtt tcaacgagtc
    12301 tgaagaagaa ttcgatgcac gtcttgacag ctacgaagaa taggggggac aagaatgaaa
    12361 acaatgcgca ggatctattc cgtcccttat attttgtggc tcggtttatt cgtgatcgcg
    12421 cctgtattaa tgatcattta tcaatctttt tttgatatga atgggcaatt cacgctgaat
    12481 aattatctat cttattttac gtcggggaca tcgctttcta tgacgatcaa ttctgtatgg
    12541 tatgcatttt tgatcacgtt tttcacgctg atcatcagtt acccgactgc ttatttttta
    12601 acaaaactga agcacaagca attatggctg atgctgatta ttttgcctac atgggtgaat
    12661 ctgctgctta aagcttacgc atttattggg attttcagtg tgcacggcag cgtgaatcat
    12721 ttctttgatt ttatcgggat cgggccgaaa cagatattat tcacagactt cagttttctg
    12781 ttcgtggcaa cgtatatcga gattccattt atgattatgc caattttcaa tgcattggaa
    12841 gagatgaatc cttctttgat cagtgctagt agagatttag gcgcgaatag ctttgagacc
    12901 ttccgacgag tgatttttcc gttatcatta aatggtgtga aaagtggtgt ccaagcagta
    12961 tttattcctt ctctttcttt attcatgctg actcgtctga ttggcggaaa tcgggtgatc
    13021 actttaggaa cggctatcga acagcatttc ttggttacac aaaactgggg gatgggttct
    13081 acaatcggtg tgattttaat cattgctatg ttcatcgtga tgctcctgac aggcgagaag
    13141 aaaaaaggag gtcgctcata aatgaaaaaa tttaaatggt cgtatcttta tttagggatc
    13201 gtctttttat tcttatatgc accgatcttc tatctgattt tttattcctt caatgaagga
    13261 ggaacgatga acagctttac aggatttact tgggaaaatt atgaagctgt ttttgaagat
    13321 acacgtttga tcattatcgt attgaatacg ttcatgctgg ctttcttatc tgcattgatc
    13381 gccacgatca tcgggacatt tggggctatg ggaatctatt acacgaaaaa acgacgtgca
    13441 cggacagcgt tgttgagcat gaataacatc ttattagtct ctcctgatgt tattatcggt
    13501 gctagtttct tgattttctt tacactacta ggattcggat taggttttca gagtgtattg
    13561 ctttcccata ttgctttcag cattccaatc gtcgtcttga tggtcttgcc aaaattgcag
    13621 gaaatgaacg attcaatggt cgatgcggct agagaccttg gtgctaataa tttccaagta
    13681 gtcaaaaata tcattttgcc ttatctttct ccaggaatca tagctggata ttttatggca
    13741 tttacctatt ctcttgatga ttttgcggtt acatttttcg tcacaggaaa tggctttacg
    13801 acgctctcag tagagatcta ttctcgtgca agacaaggaa tcagtttaga aatcaatgcg
    13861 ctaagtgcgt tagttttctt gttctccatg atattggtca ttggatacta ctttattagt
    13921 aaagaaaacc gtccaaaacg gctgaagaaa aacagaagag tacgcgaggg ggtggcacgg
    13981 ctgcgatgaa aaaattacaa tctctgtttt taggtattct catcattatt gcggtgctgc
    14041 tgcttggtgt tcgtcaactt gaaaaaacaa gtggcatgag tggagcaaag gtacttacca
    14101 tctataactg gggagattat atcgatccta gtttgatcac gaaatttgaa aaagaatatg
    14161 gatacaaagt gaattatgaa acatttgatt ctaatgaagc gatgttcaca aaaatccagc
    14221 aaggtgggac aaactacgac atcgctatac caagtgaata tatgatccaa aaaatgatca
    14281 aagaaaaact agtacttcca ttagatcatt cgaagatcaa aggattaaat aatattgatc
    14341 cacgattttt agacttggat tttgatccag ataatcagta ttcgattcca tatttttggg
    14401 gaactttagg gatcgtatat aacgataaat tctttgatgc aaatcaaatt aagcattggg
    14461 atgatttgtg gaagccggaa ttaaaagaca gtttgatgct gattgacggt gcacgagaag
    14521 ttatggggct ttctttgaat agtttaggct attctttgaa tagtaagaat atggcacagc
    14581 tgacagaagc agcaaataaa ttgaatcatt taacaccaaa tgtcaaagca atcgtggcag
    14641 atgagatcaa aatgtatatg atcaatgaag aagcctccgt ggcagtgaca ttttctggcg
    14701 aagctgccga tatgatgtgg gaaaatgaac atttacatta tgtgatccca ccagaaggat
    14761 ccaatctttg gtttgataat atcgttatgc caaaaaccgt gaaaaataaa gaaggtgcct
    14821 atgatttcat caactttatg ctagaaccgg aaaatgcggc acaaaatgca gagtatattg
    14881 gttattctac accaaatgaa gaagcaaaag cgttattgcc aaaagacatt tcgtcggacg
    14941 aacaattcta tccatctgat gacacgatct cccatcttga agtctacaaa gatctaggat
    15001 cgaaatacct aggcatctat aatgacctat tcttagaatt caagatgtat aggagataat
    15061 ggcaaaactc agtaagaatg gggacttatc aacgttctaa agagctaaaa atcgtccttt
    15121 ttctatctga gttttccaat cttctaccaa aagcggatga aacgccgctt tttttgtgtt
    15181 attttatttt ttctaccacg tttttctacc agagaaaagt ggacaaaacc attgtaaaat
    15241 agggcccgag acgggctagt aaatctaaga ttgaaaaagt tacagaagga taaagtaatt
    15301 ggaaactatg caaatatact taacccttct gtaatctatt aataaaacta atatatgaat
    15361 ccagttcaat atttgtagct atgaaaaaac ataaaaatca tattagataa aaactaaatt
    15421 ggtagataaa ttattttgtc agatatctga ggcaaatatt cgactatttg tgtttcaaag
    15481 taaaaattgg aatacaaata tagaatagac atattgcagc cgatagttta ttatgctttt
    15541 agacacatta aactggagga aaaaatgaaa aagagcgcta aagatcgagt ttttgatgag
    15601 ctgatacgac taacagggaa tatgtcttct gtcactgccg agaaaatttc gaagaatcta
    15661 ggtatctctc gtcagaatgc cagccattat ttgacgagat tagtggaaga taaaaaggtc
    15721 gagaaattac cgggaaagcc tgttttgtgg aagcctttag atgaatatgc ggttgtagat
    15781 aatacggagc aaataaacga agcctttcat tctgtagttg gtcatgacgg aagtctgaga
    15841 gaggtcattc aaaaatgtat agccgctgtc aaatatccgc ctaacggact cagtgtatta
    15901 ataaatggag ccacgggtgt tggaaaaagt tttttagcca caaaaatttt tgaatacgct
    15961 atccatgagc aaattattga aaaagatgca ccatttgcta tcttaaactg tgccgattat
    16021 gcggacaatc ccgaactgct ttcggcaaca ctgtttggat acaagaaggg ggcctttacg
    16081 ggggctgaaa aggataccga tggtttattg gcaacggcca acaacggcta tctttttctc
    16141 gatgaggtcc atcgcctatc gaaagaaaat caagaaaagc tctttctatt tattgatact
    16201 ggaaactatc gtccagtcgg tgaaaatgta aattggcatt ctgctaaggt tcgttttatt
    16261 ttcgccacga cggaaaaagg tgaaaattat ttattagaca cctttgatcg gcgtattcaa
    16321 attagtgtta tgttgccaac ttttgaagat cgccccattc gagaacgatt agagctgatt
    16381 caattatttt ttcaaaatga agcggatgtt ttacaaaaag atattattgt ttcaagagaa
    16441 gccttgtctt taatgttatc tcatcccttt agtgggaata ttggtaagct gaagaatatc
    16501 attaaaatca gttgtgcgga tgtgtatagt cgaacacaag aagaaccatt aacgattggg
    16561 aaaaatgaga tcttgattca gttaaatatg ctggaatccg aagtagaaag cttgccgatt
    16621 gctagtttat tgataaagaa aaatcaaaag ataaaaacgg atcgaatcaa accgtttgat
    16681 tatgattcga ttttggaaaa aattactcag ctgataaatg aaggtaattt caatgaaaat
    16741 ttttcttcga taaagaatga agtacagcat ttggtgcatc acattgataa ggattctttt
    16801 ttatcaatga gtagaaaatt gatgattcag atgtttagaa aggtctgggt atccgtttta
    16861 ggaaaaacgt acggattgac gacgagtgga cctgttgcag aaattattag cagatcctat
    16921 gtcatttccc caagctataa gacagactta accgggataa atcgtacatt agctgcaaat
    16981 ctacctcgga cgacttactt atgtaatcag ttcttggagc atttaccacc gctctcagaa
    17041 aaggacgaga actttctaag actgcttatt gtcgtgactt tatctgatta tgtagatgaa
    17101 tccatcgagc tgaagggcct gttgcttgct cacggagata gtacggctag cagcattcaa
    17161 gcagtagtga atcaattatg cggcaattat gtttttgaag cgttggatat gccagtagag
    17221 agtaatgtgt cagagattgt cgaatcgaca aggaatttcg tgaaacggca agtgaataca
    17281 gatagtctag tgctgatcgt agatatgggc tctttaaatc aaatttacac acagatcaag
    17341 aacgatttaa aaggggagtt acttcttctg aataatttga cgacttccat tgcacttgat
    17401 attggcttga agatgacgat gaatgcgcca tttaaacaga ttgcggagga tgcagcagac
    17461 aagtattcaa tcaatgttca atactttgaa ggcttttctc aaagtagcaa cattatcatt
    17521 tcctgtatgt cgggtttggg gatttcggac aaattgaaag atgtttttcg tcatcattta
    17581 tcggatgaat ccatcgaagt tttcacgaag gactacagag aactacgtga attaatcgac
    17641 caaaatgatg aaacttattt tgacaagaca aaattggtca tcacgacttc ggatcttcca
    17701 cgttcctttt caattcctaa tattaatgtt tatgacatct tagacggtga tggagcagcc
    17761 aatttggagc atattttgag tcctgaaatg aagccggcag aatttaaccg cttgattcag
    17821 gagctagtaa aattcttttc gttggaagga attgcggacc gtttgaactt tctgaaccca
    17881 acgatcgtga tgagtgaagt ggaaacagtc atttctaaat atgaaaatta ctatcatttc
    17941 actttaaatg gccgtgtaaa actaaatctc tatatgcata cggcattgat gatggaacgt
    18001 ttattcttgg ctcggacaga aaaatctgaa gaaccgaatt atgatttgaa accagatgaa
    18061 gatgaatttt attatgtttc tagaagtatt tttcaatcaa tggagatgaa atataatttt
    18121 aaagtcaatg cttatgagct ttcgctgctc tatgaattat taggaccgta tattcaaaaa
    18181 taaagtgtct aaaaaagtgt ctaatctgtt ttttttaggt tagacacttt ttttaatgtg
    18241 aattaacgtg attaaaccaa tgtttttaaa agttggcaca ggaattgctt atataaaagt
    18301 gaacagaaag aaaggaatta accatatgaa gaaaattcta atagcgactc acggtcactt
    18361 agcttcaggc tttcttagca gtattcaatt actgacgggg aaggttcaag agattacggt
    18421 gattaatgct tatgtggatg agtgtgattt tgaacaggag ttgaatgtat ttgtaaacca
    18481 ggtgaacgag caggatcaga tttttgtttt taccgattta tttggcggaa gtgtcaatca
    18541 gaagattacc aaaacctttt tggagaaaga gattgctgta actattttgt ctggctttaa
    18601 tctgccaatt ctgctcgaaa tagtattggc aaccaaggaa ttaacaaagg aggatattca
    18661 ggaactggta aataaatgcc aacaggaatt aaagatgacc attcttgatg aggtacatgt
    18721 agtaaatgaa gaggaagatt ttttttagga ggaagaaaaa tgattaaagc agtgcgaatt
    18781 gatgaacgat taattcatgg acaagtagcg atgacttgga caaagagtct aaatttaacc
    18841 gggttagtag tagcaagcga tgaggcggct accaacgata ttcaaaagat gacattgaag
    18901 atggctgcac catcgaatgt caaagtcatt attaaaacag ttgatgacgc gattcatttg
    18961 ttaaaagatc cgcgggcgaa ggacatgcgc ttgatggttt tagtccctaa tgtcaaagat
    19021 gcggtaaaag ttgcgaaagc tttcccaacg gaaatcgaat taatgaacgt ggggaatgct
    19081 gggaaaatga caccgccatc ggaagataag aaaaccttat caaaggaagt gatgttaacg
    19141 acgtcagaaa ttcaagactt aaaagaatta gttgaacttt atccagatac cgtttttcag
    19201 cctacaccgg cgatggaaaa acgttcagct atctctattt tgagtgattt ttagaaaaag
    19261 gaaggagaaa aattatgtat gatgcatttg tagtcgcttt ggccgttttt attggagttg
    19321 ctggacatga aatgttcggg atgtccatgc tgagtcgtcc gattgttgtt gctccgttag
    19381 caggagcgtt gatgggggat ttgcagacgg ggttgattgt cggggcgtca ctagaagcca
    19441 tctttatggg tgtcgtcaat atcggaatct ctagtacggc ggagccttct ttggctgctg
    19501 ggctggctac agcgttcgca atcaaaatgg gaggaaacgt ggatgcgatt attcccattg
    19561 tctttccact agcagtatta ggattacagt taatgaatat gattttttct tttgtatgtg
    19621 gaccgatggc tgctaaattt ttcaaatttg caaaatctgg tgatcaaaaa ggaatcatca
    19681 atctgcactt ctttatgtgg tttgtccact atgcgcttta tgctcttatt ccattctttg
    19741 ccgttttatt tggagcagat gttgtcaaaa gcgtcttgga taatattcca gaggtcatca
    19801 tgaacggatt aacagtcgct ggaaatctgc tgccggcagt tgggatggcg atgctgttac
    19861 ggatgctttg gagtaaaaat attgctgtgt ggtttttctt tggggccgtt gttatggcgt
    19921 atctcgagtt accattgatt gcaattgccg cagttggaac gattattgca attgttatag
    19981 cccaaagaga tctacaacta aaaaatatat ctgttcatgc agttcaaagc gatgctgctg
    20041 taaatgcaac gcttgaccaa gaagaggagg atttctttca atgaaattag ctgatggaat
    20101 ttctaaagat gagaaaaaat tggttcggaa gatgttttgg cgctcgatga ctatgtatgc
    20161 ttcggtgaat ccggtgacga tgggcggcgg tggtttcgcc tattctatga tgcccttcat
    20221 taatcatttc tacaaaagtg aagaagatcg agctgcggcg ctggaacgtc atacacagta
    20281 tttttcgacg accattcctt gcgcgtcctt tgtcatgggg attgcggcat cgatggagaa
    20341 ggaaaattct gaaaaagctg gttttgacgt ggaaagtatc aacgcgatca aaacgagttt
    20401 gatgggacca ttggctggaa ttggcgattc attgttctgg ggagtttggc gtgtcgttac
    20461 cgcgggacta gcgatcaact tagccaatgc cggaaatatt ttggcaccaa ttatcttttt
    20521 agttatgttc aatataccta attacttgat gcgatactat ggcggcgttt taggctattc
    20581 attaggctct aaatatattg aaaagctgta tgcgaacggg ttaattgaaa ttctgacaaa
    20641 agctgctagt atcttgggtc tgatcatggt aggtgcaatg acgatcagta tggtcaaatt
    20701 caataccgtt ttgagctttt caatgaaggg cgaagaacta atgaatgtcc aagaaatcct
    20761 cgatagtatt tttaaaggaa ttgtaccgtt attggtaaca ttgagctgtt tcaaattact
    20821 agataaaaaa gtcggcgtca tcaaaattat ttttggaatg gtcgtcttgg gaattttatt
    20881 gtcattactt ggaatcgcat agtaggaagc tgcagctact aggctgcagt ttttttagca
    20941 aggagggaaa agatgactat taatgaagta atttattcac ccaaacaccc attttttaat
    21001 gctgagttaa acgacggaaa tgtgactact tcaaacgaat acagctacta tcggaatatt
    21061 cctgatgtaa agggattgac cttcgatgta agtaatcaaa agacattagc aaaccttgat
    21121 gtttgcggga cgattcaaac gttgaccttt tatcgagaaa atttactaac tgaggaaaaa
    21181 ccaggtgttt gggtcaataa acagctctct caaaccaatt cgttatcctt aaaggttgaa
    21241 gtaaacggga ttgtctacca tttgtcagaa tcggatcatc gtattgaagt cgatgtgatt
    21301 caagactgtt tgcctagata tattcatcat tattcgacgt ttaaggtcat tgtagtatcc
    21361 tttgctccca tactggctaa taagcgttta agtatgcttg ttcaacaggt ttacattgtg
    21421 aatgaaacac acgaaagcct tcaagtttca gtgaaagatg tgccttcgta tcaagagaag
    21481 tattcggatc agcaaaatgt gttgatttct caaaaaggaa ataaggacac gatcgcacaa
    21541 caggaagtgg caagcttcgc cattgctttt gtggacccaa atgcttttga agaagtaatg
    21601 acttttcagg aatctgatat cgaaaaatgg ctgaaggaaa cattgaacta ttttgctcag
    21661 ctatttgggc agctgtcaat gccaaacgat agagttgtcc atctctatcg tcgtgcactt
    21721 tatcaaagct tttctagttt cggtatgaat cgacagggac aggtcgtcgg cagcaattgg
    21781 ggcagctatc ctgctactaa tcgaatttgg aataaggata tgtattacgc atcattgcca
    21841 ttcaccatgt ttgaaccgga gttatgtcaa aaaacggttc tatggtttga tcaatatggc
    21901 gttaagtttc ctggttcaaa atttacagga ggtatcaatc attctctgag taattcgtta
    21961 tccagtgcgt tactggctag tctttatttt gaacatactg gggatacaaa attctttgag
    22021 cggtgtccgc aggttctact gaatgcttca agaattattg aggacatatt agctcaacgt
    22081 cggtcagggg aaccaatgct ttttccatct gtttggttat cagacgcctt tgcacttgga
    22141 aagtatcata ccggcagcaa tctttgtctt tggaaggcct gtattggact tgcagaaatt
    22201 tttgaagccc tagaacaact gagtcttagc aagcgataca aaaacattgc ctgtaaacta
    22261 aaggaatcaa tttgtaaaca aatgactatc gatggtcctt ttggtgagca atttttggag
    22321 ggaatcggtg atgaggagaa aacaacctat tcagtgaagc actatcaaaa accgattctt
    22381 gagcaagggt taatcttttt aagtgatgtg attgtggatg gaaaaattaa tttattgatg
    22441 catgatggag aagaatcaga cactacgttg atgccttttt atcaattttt agataataaa
    22501 gatcatttat atcagaacac catggccttt tctgctagtt ctttcaatcc aacctatagc
    22561 gaggaaatca aaggcatcac ttggggatta gaatccggcg ccacttttcc tggctttatc
    22621 actgttttaa tgagcgagct gcacaaccct caagctttcg taacccgatt agaagaacta
    22681 atttgtttgg cagatttaga cggttcgtgg tggtggtggc cgtatcgctt agaggctcaa
    22741 caaggagaag tcgtgcgaga ctttggctgc ggcaaatgcg gctgggcatc gggtcttttt
    22801 gtcagcctaa tgatcagtca atatttcgga ttgaaattca aaaaaggagt attgcaaatt
    22861 gatcctgttg ataatttaac gttttcttgg aagaatttga agttcggtca agccttcatt
    22921 tcgattgaat gtacgcaatc cgagttgagt atttcaaatt tgtcagaaaa gccattgaaa
    22981 ataagtgata cgaaaaaaat tcttcacgta gaaaagggaa aaacgattca cattcaaagg
    23041 agaaaaagat gagtatacta aaaaatatta cccaatttaa aaaaatccag agtcgagcaa
    23101 tcactcccga aaatccaaca ggcgagaaag gtaaagctgc gatgttgcca agcgagctag
    23161 gaccaagccg caaaggtcgt ggcgcaattc cgctgacttc tggtgaagaa actgtgattg
    23221 cggatattac tggaacggga ataattcgtc atatgtggat gacgattcgt gatcgaacag
    23281 aatttggcag ttttgtttta cgtgatttaa tcttaagaat ttattgggat gggtcggata
    23341 gccctgctgt tgaaagtcct ttaggggatt tcttttgtaa tgggtttgga gagcgttgcg
    23401 aggtgaactc gttgccgata gtggttaatc ctacaggggg ctttaattcc tatttcgaaa
    23461 tgcctttcca aacatccgct aaaattacga tcgagaattt acatccgaag gatatgtcct
    23521 cattttttta tacgataaat tacgctttgg tggacgaatt ggaagaaaat agcctatatt
    23581 ttcacgcaaa ttggcatcgg gaacgcatta ccaaattaca ggaggattat aagttaattg
    23641 atcgattaaa gggtcaaggc tattacgtgg gtacttacct tgcgcttacc tgcctagaac
    23701 gttattggtg gggcgaaggc gagtttaaat tctacattga tggggatagt gagtatccga
    23761 ccgtaacctc aacaggttca gaagattatt ttggcggggc gtgggccttt catgagtacg
    23821 atgagttcgg acgtccacat gcgaaaaact attcaacagc ctttttaggg tatccttacc
    23881 aatcaaatag agatgccaca agagatcgtt ttgaaacggg aaaattgaat gccgttcatg
    23941 cctttggtga cgacgcaatg ccacgacatg gtctttatcg ttggcatctt caagacccca
    24001 tctgttttac cgaagattta tcggtcactt tacaacaaat cggcaacgat gatgttcgct
    24061 tatttgagcg gcaagacgat gttgctagtg tcgcttactg gtattcaaca gaagcttcag
    24121 gaaatactaa acgtcatttc actgatcgag aacgcttgcc gcgttaatga agtgtttttc
    24181 acatattcga agggaggaac ctatgatgat atatggggca attgaagctg gagggactaa
    24241 atttgtctgt gcagtcggta ctgaggaatt aacgattttg gaaagagtca gttttcccac
    24301 aacaactcca gttgaaacaa tgaagcaggt cattgatttt tttaaacctt ttaaggaaca
    24361 attggcaggc atcggggtcg gctgctttgg tccaattgat attcaatgca aatcaacgag
    24421 tttcggtcat atcacatcaa caccaaaact tgcttggcaa aatttcaatt ttgtaggaac
    24481 gctaaagcaa tatttcaata ttccgattga atggacaaca gatgtcaatg cggcttgcta
    24541 tggtgaatat gttgctggtt ctggcaaagg gttagcaggt gttgcatact tcactgtggg
    24601 aacaggtatc ggaggtggtg ccttgttcaa tgggactttc gtggaagggt tcagtcatcc
    24661 ggaaatgggg catatgttga tcaagcgaca tccaaaggac aatttttcag gaaactgtcc
    24721 cttccaccaa gattgtttag aggggatggc agcaggacca gcgattgaaa aacggttggg
    24781 tgtaaaaggc cagaatttat tggctgacga ttcgttttgg cagattgaag ccttttacct
    24841 tgcccaatgt gcatacaata caacactgat gttttcgcca gatagaatta tttttggcgg
    24901 tggtgtaatg aaacaagaac acatgaagaa aaaagttcag gataaatttg ttgagcttat
    24961 aaatggatat gttgaaatac caccaattga tagctacatt attacgcccg aattaggtga
    25021 taatgccgga ataatcggcg gattagcatt ggccagaaaa gcggtaagaa ataagcaacc
    25081 ataattaatg aatgtttcat tgatactcat gtcaaaagta tttaaccgag taatcaaatg
    25141 atcaaatgaa gttggataaa gcgaaaatga attcaaataa aaaggctcta agtcaaatcg
    25201 tgttgttttc attaaatcac tgaaaggaaa tgcttctgcg tttccttttt tctttttgtt
    25261 gtgcgctgta taccttgagg atattcactg gtggcgtaca cttaaatagt aatatattgt
    25321 gttactttat gtctgtggta atagttatag ttgcttacca atgtatgttc atttttcgaa
    25381 atgttgactt cataatcctt ggcttaatgt ttgaattttt ttggtgatag attttttcag
    25441 ccgtataaaa ta
//
LOCUS       DAFGZE010000038        24754 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.039, whole genome shotgun sequence.
ACCESSION   DAFGZE010000038 DAFGZE010000000
VERSION     DAFGZE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.039"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            67..444
                     /locus_tag="LTU54_001688"
     CDS             67..444
                     /locus_tag="LTU54_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296403.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            complement(624..1919)
                     /gene="purB"
                     /locus_tag="LTU54_001689"
     CDS             complement(624..1919)
                     /gene="purB"
                     /locus_tag="LTU54_001689"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBL8296404.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1932..3056)
                     /gene="purK"
                     /locus_tag="LTU54_001690"
     CDS             complement(1932..3056)
                     /gene="purK"
                     /locus_tag="LTU54_001690"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBL8296405.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(3049..3537)
                     /gene="purE"
                     /locus_tag="LTU54_001691"
     CDS             complement(3049..3537)
                     /gene="purE"
                     /locus_tag="LTU54_001691"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBL8296406.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3953..5260)
                     /locus_tag="LTU54_001692"
     CDS             complement(3953..5260)
                     /locus_tag="LTU54_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBL8296407.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(5263..5844)
                     /locus_tag="LTU54_001693"
     CDS             complement(5263..5844)
                     /locus_tag="LTU54_001693"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBL8296408.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5960..6057)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(6416..7708)
                     /locus_tag="LTU54_001694"
     CDS             complement(6416..7708)
                     /locus_tag="LTU54_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8296409.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7705..8328)
                     /locus_tag="LTU54_001695"
     CDS             complement(7705..8328)
                     /locus_tag="LTU54_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296410.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(8331..9167)
                     /locus_tag="LTU54_001696"
     CDS             complement(8331..9167)
                     /locus_tag="LTU54_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296411.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(9184..10071)
                     /locus_tag="LTU54_001697"
     CDS             complement(9184..10071)
                     /locus_tag="LTU54_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8296412.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(10087..11370)
                     /locus_tag="LTU54_001698"
     CDS             complement(10087..11370)
                     /locus_tag="LTU54_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8296413.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(12053..12736)
                     /locus_tag="LTU54_001699"
     CDS             complement(12053..12736)
                     /locus_tag="LTU54_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL8296414.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12754..13371)
                     /locus_tag="LTU54_001700"
     CDS             complement(12754..13371)
                     /locus_tag="LTU54_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL8296415.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(13381..14205)
                     /locus_tag="LTU54_001701"
     CDS             complement(13381..14205)
                     /locus_tag="LTU54_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBL8296416.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(14198..14758)
                     /locus_tag="LTU54_001702"
     CDS             complement(14198..14758)
                     /locus_tag="LTU54_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296417.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14932..15888
                     /locus_tag="LTU54_001703"
     CDS             14932..15888
                     /locus_tag="LTU54_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8296418.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(16193..17149)
                     /locus_tag="LTU54_001704"
     CDS             complement(16193..17149)
                     /locus_tag="LTU54_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HBL8296419.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            17253..17855
                     /locus_tag="LTU54_001705"
     CDS             17253..17855
                     /locus_tag="LTU54_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL8296420.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17943..19442)
                     /locus_tag="LTU54_001706"
     CDS             complement(17943..19442)
                     /locus_tag="LTU54_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBL8296421.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(19458..19982)
                     /locus_tag="LTU54_001707"
     CDS             complement(19458..19982)
                     /locus_tag="LTU54_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBL8296422.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            20280..21029
                     /locus_tag="LTU54_001708"
     CDS             20280..21029
                     /locus_tag="LTU54_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBL8296423.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            21020..21805
                     /locus_tag="LTU54_001709"
     CDS             21020..21805
                     /locus_tag="LTU54_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HBL8296424.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21774..22490
                     /locus_tag="LTU54_001710"
     CDS             21774..22490
                     /locus_tag="LTU54_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL8296425.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22592..23626)
                     /locus_tag="LTU54_001711"
     CDS             complement(22592..23626)
                     /locus_tag="LTU54_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HBL8296426.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(23630..24688)
                     /locus_tag="LTU54_001712"
     CDS             complement(23630..24688)
                     /locus_tag="LTU54_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBL8296427.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         7356 a         5316 c         4318 g         7764 t
ORIGIN      
        1 tacttaatga tggtatatta gaattaacta aaaatttgga taatgggttc gaggaggcac
       61 ttgccgatgg aagatgcaat aaaacaaggt cagctttttt tatcagaatt aacagctgaa
      121 gaacgttcct gtctatataa tgatttgctg tcgatcaaag acgtttattt atgcaataaa
      181 gatactagtt tttatacatt ccgaaaaatc cgccgagcaa ccaagcgctg tgctgaatgc
      241 cgtaaaagga cttttcttta tcagacagaa cacaaaggcg aactttcaat cagtcagatt
      301 catgaacaga agcgtttcaa aaatcattat cgtatccaga tcttattcaa tggacaagag
      361 atggaatacg cttttctcga aaacttgctg caacatctcc ataaccagca ttttagttat
      421 aatagccaag ccatttacgc ttaattcctc tattttttgt atgtaaagag gcacggtaaa
      481 gaggtagccg cttttaaaat caggttccta ttattgataa gaaaatataa taaactaaaa
      541 gaagcagaac cgctaacgga tctgcttctt ttttggttat ttttgctatt ctattttaat
      601 tgaattttgc aactcactct tctttattcc aaacctactc gtttgaatat cacgtcaacg
      661 tttttcaaat gatgatgata atcaaaggca tcgtctattt cttctttaga taataccgat
      721 gtgatggttg gatcttcttc taacagcggg cggaactgca cttggttgtc ccaagcgtac
      781 gcagtttttg gctgaacaag atcataagct tcttctcttg tcattccctt atcgatcaat
      841 ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg cagacatatt ccgcttcatg
      901 ttttcaggaa agaccgttag atttttaacg atattgccaa aacgatttaa catgtaattc
      961 aataaaatcg tggtatcagg caagatgatt cgttctgctg aagaatgaga aatatctctt
     1021 tcatgccata atgtgacatt ttcataggca gtgaccatgt gtccgcgcac cactctggct
     1081 agaccagcca tattttctga accgatcgga ttccttttgt gcggcatagc agaagaacct
     1141 ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt cggatttttg caatccgcgt
     1201 atctctgttg caaaacgttc aatacttgta gcaataagcg ccatcgctga gaaatattca
     1261 gcatgtagat cccgagggag aacttgagta gaaatttctt gtggacggat ccccaattgt
     1321 ttacatacat actcttcaat aaatggcggg atatttgcga acgttcccac tgctcctgag
     1381 atttttcctg cttctactcc ttttgccgca tgttcaaatc gttcgatgtt ccgtttcatt
     1441 tctgaatacc atgtggcaag tttcaatcca aaagtagttg gttcagcatg gacaccgtgt
     1501 gtccgaccca tcatcactgt atatttgtgt tcttttgctt tttcgccaat gatttccgta
     1561 aaacgtttca agtcttcccg taaaatatca tttgcttgct tgatgagata gccataagca
     1621 gtatctacga catccgtact tgttagacca taatggaccc attttctttc ttctcccaat
     1681 gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc gtgttaattt ttcgatttcc
     1741 aagatccgat cgatatcaaa tgatgcatgc tcacgaattt ttttcacatc ttcttgcggg
     1801 atttcaccaa gttctgccca agcttcatct gctaaaatct cgacttcgag ccacgcacgg
     1861 tatttgttct cttccgtcca gatattcccc atttcagggc gtgtataacg ttctaacatg
     1921 ttttttctcc tctaatccca aatattcgtt tgtttgattt cttccagcgt ttgctggatg
     1981 tcttctgtca agatagtgat atgtcccatc ttgcgacctt tttttgcttg tttttttcca
     2041 tagaaatgga attgccagtc tggcttattc tcaatcaagc gatagctttc cagcagttgt
     2101 tctcccaaaa cattgaccat tactgcattg cttatcaaac gtactttttc tcctaatggc
     2161 caaccacaaa tacctcgaat atgggcatcg aactgactca tagaacaagc ttcaatagaa
     2221 taatgtccag aattgtgagg acgaggagct aattcattca catatagccc gcctgttttt
     2281 gttaaaaaca tttctacccc taaaacacct gaaaggctca ctgcttctgc aatcacacgg
     2341 gcaatacgct cagcctcctc gatcacatcc tgatcaatcg cggctggagc aatcgtttca
     2401 tgaagaatat tgttgtggtg gatgttttcc acgaccggaa aagtcgtgaa atctccttga
     2461 ccatttcctg ccaccatgat cgaaatctct ttttcaaacg ggatccatgc ttctaaaaca
     2521 caggttcctt ctcttaaaag gtccatggca ggagctagat ccgcccgact tttcaaaaca
     2581 tactgcccct tgccatcata gccgcctcga gtggttttca aaacacaagg atagccgatc
     2641 ccgtcaatcg catcttggat atccgtcgga ctgacgatcg ttgcataagg agcaatcacg
     2701 atgttattcg tttccaaaaa agatttttct aacaggcggt cttgcgtaat cgcaagcaag
     2761 tctgtccctt gaggaataaa tgacatgggt aaaatcgcat ttaacgcctc tacactgacg
     2821 ttttcgaatt cataggtaat gacatctgtt cttctggcaa gctctttcag cgcaaaggtg
     2881 tcatcataga cactttcaat atgccaatct gcaacttgtg cggctggaca atcgctcaca
     2941 ggatccagca cacctacttt aaagcccatt tcttttgcac taatcgccat catacgacct
     3001 aattgaccgc caccgacgat cccgatcgtt gaaccaggca acaaaggttt aaccaagttg
     3061 atcactactt tctataacag ttttagccaa cattgaacgt ctttcagcca atttagcctc
     3121 caattctaaa tcatacatgg aaagcatctg tatcgcaagt agtccagcat taattgctcc
     3181 cgcttttccg attgctgtag tagctacagg tactcctcca ggcatttgga caatcgaaag
     3241 aagcgaatcc aaaccattta atgttcgtga ttggaccggt acaccgataa ccggcaaagt
     3301 tgtttttgat gcgaccatac ccggcagatg cgcggctccc cctgctcctg caataatgat
     3361 tttgattcct ttttttcgag cgttttgtgc atattcaaac atcaaatctg gggttcgatg
     3421 agcagaaacg accttttttt cataagacac accgaattca tccaatattt gacatgcttc
     3481 tttcattgtt tcccaatctg aagtactccc cataatcaca gagatttttg ctgtcattgt
     3541 ttttcctccc agtttttcta cattcatatt ttaacaatca ctttttttga tgtcaaagaa
     3601 atttcgaatg ttaacaaaga aaaacatttt attgttcgtg tttattaaag ataaaaaaaa
     3661 ggaatgtgac aaaagttagg tcacattcct ttttatggat aaacggtgtt ctcctgccaa
     3721 ctcctcggtc acaaggaaca atattcaaca aacatgagaa gctttttttt gaaattgttc
     3781 cttgtgactc ggagatgaac ggcaggctca cgacctcttc cttcaacggt gttcaatcag
     3841 ctgaccctcg gtattagctc aaacaaacgg caaaatacga ggaactattt ttccgtttgt
     3901 ttctctaaca atcaggtcaa aacgctgatt tcacaacctt tcttttgctc tattacttct
     3961 tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc attaaagatc aaattcaata
     4021 tgatagccgt aatgctactc attacgatgc catttccagt gaacatttgc aatgtttgcg
     4081 gcatttgact gaataatgtc ggcgtaacat tgaaacccag cccaaatcca acagaaacag
     4141 caacaatcaa tagattttta tcattgctga aatcaacttc caacagcata cggattcctt
     4201 ggactgccac catcccgaac atgactaaca ttcctccacc aagtaccggc tcagggatga
     4261 tttgtgctaa cgcaccgatt ttagggaata atcctaaaac aatcaagaag aatgcagaga
     4321 aatagatggg ttttcttgtt ttgattcccg aaagttgaac caatccgaca ttttgagaaa
     4381 agccagtata agggaatgta ttgaaaatac cgcctaagat aacggcaagt ccttctgcac
     4441 gatatccctt tttcaattct tcttctccaa catttttccc agtaatgtca ccaagtgcaa
     4501 aatatacacc tgttgattct accatgctga ccatggaaat gatgatcatt agtaaaatcg
     4561 accatacgtc gaaagttggt ttaccaaaat aaaagggttg tggaacatgg aacaacggcg
     4621 catctccaat cgcgctcaga tcaatcattc ccatgaaagc agctaagatg cttcctccga
     4681 ctaaaccaat caataccgcg attgaacgaa tgaacccttt tgccagcatt tgcacgccta
     4741 caattaggag aatcgtcaca aatgcaagta gcagatttgt tttgtcacca aaatcagctg
     4801 ctgttgcatt acctccaccc attttctcga ttgctacagg aatcaaagtc aaaccaataa
     4861 cggtgataac cgttcctgtt actataggag ggaatagttt tttaatttta gaaaagacac
     4921 cagaaactaa cacaacaaag atcccagaag cgataataga tccgtagata gctcctactc
     4981 cttcattggt tccgatcaag atcagtggag ctactgcttg gatcgcacat cctaaaacga
     5041 caggcaagcc aattccgaaa aatcggttaa ctgtcaattg caataatgtc gctaatccac
     5101 acataaagat atcaattgaa atcaaataag tcatctgttc ttgattaaag cctagtcctg
     5161 taccgatcaa aagaggcacc gctactgctc ctgcatacat cgctagcaga tgctgcagcc
     5221 ctaaaaccgc agcttttcca ttttgtgttt ctttttccac gattatgcgt cctcctcaag
     5281 aaactcaact tgtccattcg tcaaggaagc aatacgcgca agagaaacga ctctcaatcc
     5341 catttcttca agtaactgac gtccatcttg gaaagacttt tcgatgacga tcccgattcc
     5401 ttctacttgc gctccagctt gctggcataa ttcgatcaat ccttttgccg cttgtccgtt
     5461 tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa gataaaaatt tacgagaaat
     5521 agaaatcgta cttgtcactt gtttcgtaaa agaataaaca gaagctgtca ataattcttc
     5581 atccatcgtc aaactttttg cttttctagc gaagatcatt ggaacatcta attctcttgc
     5641 agcaaataaa gctggtgcga tcccagaagc ttcaatggtc acaactttcg tgattccttg
     5701 ttctttaaat acttctgcaa accgttttcc catttgttcc atcaatacag ggtctacttg
     5761 gtgagtgacg aagctgtcga cttttaatac gccctcgcct aatatacgtc cgtctttttg
     5821 aatacgttca actaattctt tcactttatt tgctccttta tcatttatta ataccgaaat
     5881 aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag aaaaacaccc tccactttat
     5941 cataagaaaa gaagggtgaa atagccaatc ttttacttat agtccagcat ttacggtact
     6001 ggctagaaac tgtcatccat attatgacga tatataagta ggtatgtaaa actaataata
     6061 gtgtaaattc attttcttgc aactgttttg cacgtgaagg cagtttgcca tatttgaata
     6121 aaaaatgttg tttcaaatat tgaaaacagt ctaacactac tttctgaaac tgacaacgtg
     6181 gtttacttat ttttttgtaa aatctaatga tttttaatgc attattcctt tttgttcggg
     6241 tttaaatagt tatttttatt ttctcctatc taaaaaagca gcccacataa aaagaaggca
     6301 tgacaaaaag catgtcatag cccctttttc cgaataaata gtgttcaaaa gacagcctat
     6361 cggcaataag gcaagatttt ataaactacg ggaagtaatt tccgtaaatc tattcttatt
     6421 cttttaagct gacaagctcg tcaatcacta ctgcttttcc tgaagcaata gaatgatttg
     6481 ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg agttgctgcc cgattccacg
     6541 gatcttcttc tttttcttca ccaaatagat catctaataa acgcggatct cctccgccat
     6601 gtccgcccgc ttcttgttcg acgattacct catatggctc accgaacatc ggatagacat
     6661 ggatggactg aagaacggct gctccttcat cctttttatc tccacctgca ttgatgtaag
     6721 acttttcgat cactttcatt tcgatccgtc ctttgctgcc attgaaagaa acattgaatc
     6781 cttcccacgg catatacgcg tttagcgaat acgtcaaaat cgcaccagtt tcatatttga
     6841 ccgttacgcc tagtgtatct tcaatagaga tatcatcgga aaatacactg cggtcacgaa
     6901 tatatccact gtctttttct gcttctaaat acatttctcg caattcctca ttggctgaca
     6961 ggtctatagc aaatggatca gctttagcta gttcgcttcc atagcatcga ctgtaaaata
     7021 tttctactcc gcgtttctcc gcattatgca tcccataaaa gtttaatcca ccgaatgcaa
     7081 ataccttttg gggacggctg ttcaaccaaa aattcactag atcaaaatga tgggtagatt
     7141 tatgaacaag aagcccgccg ctattgtgct tatttcgatg ccatcttctg aaataatctg
     7201 ccccgtgttg cgtatttaac agccattcga agtggataga aaacacatca ccaatcacat
     7261 cactttctat cagttccttt atcttcgtgt gatacggtgc ataacgataa ttaaaagtga
     7321 ctcgcaggct tcttcccgtc tgtttttgga ccgttattat ttcttgagct ttttcctcat
     7381 cgatagtcag aggtttttcg caaataacat catatccttt tttcattgca cgaatgatat
     7441 aatcatgatg cgttcgatct actgacgtaa taatgacttc agccggccgt acgtcatcca
     7501 gtagttgctc aaattgtgat ggcatacatg tctttacttt tttctgtcca tacttgttca
     7561 caagtatccg atttgcataa tccatccttg tttgagatac atcacaaaaa ccgactagtt
     7621 cacaagtttg atgatacgta gaggcaattg cctcataaaa aaaacgcgct ctcccaccaa
     7681 gtccgacttg tacatatttt ttcttcattt aaagtcattc tccttttctt ttaagcaaaa
     7741 tactgtgaca aatggcagcc ctggatacag taatgcagtg agtaaaaaat catgctgtat
     7801 acaccagaac agtccataca aaaggcataa ataccacaga aaccccttga cgttttttga
     7861 caacagccag atattccttt tgatcaaata aacaagacta tctgctttat cttctttgca
     7921 gatatcaatt agcgtactaa aaaaactatg ggatacaaaa agcaacagac acaatagaaa
     7981 aggaatcaga taccaagctc caatctttcc cactaataaa taccaatcaa ctgcaaaaat
     8041 aatgacaaac agctgaaaca acgtaatgat gaaacacatc cgtttgattt ttcctttcct
     8101 ccaacatacc tttttttctg ccaagtaatg gacagataga taataagaag gatacatcat
     8161 caaaaaataa atcagataga caacacgagc agacagcact aatcctaaag tgaacaaaca
     8221 tcctagaact aaagcgaaaa gagcattact gataagactg gttaaaaaaa tccgacagac
     8281 atagtagttg agcttgtaaa gaaacatctc ttctattggc tttttcatat tcagcccttc
     8341 agaccgcttg tactgatccc ctggacaaga tatttattga agaataagaa aatcagaaaa
     8401 accggtagca gcgacaacgt agacatcgca aacatactcg cgtaagtcgt actagatcca
     8461 gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag atctcggctt gtttaaataa
     8521 atcaatggtc ccatgaagtc gtcccatttc caataaaact ggatgatgat cgtcgtgatg
     8581 atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga cactatactt gctgcatccg
     8641 tcaataatcg ctgattcatc taactctttg gggatgctga ccatgaattg catgatctgg
     8701 taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca gcggcacata cgtcccaacc
     8761 aaattcagcc gattgtaaat aataaactgc ggaatcaaaa tcacttgtgc aggaatcatc
     8821 atcgttacta acatgacgac gaaccagaat ctttttcctt tgaaatcgat tctcgataat
     8881 gcgtaagcaa tacaagtcga gctcagaact gtccctatcg tcgaaactgc agagatgaca
     8941 aacgaattcc taaagaaagt aaaaaatgaa attccagcaa accccgccca tccttttgcg
     9001 taattttccc attgaaaagt ctctggaatc aaattcagcg aaccactcat aatctcttga
     9061 tccgttttaa aagaacctgc gatcatccat aagaccggat aaatcatgac tagacctaat
     9121 agcaaaataa aaccgtattg aaaccatttc gttactgtat tattcatttc ttcctcctct
     9181 cattcacaca acgacccatt tttttgatga tttgaaaatc agaaaggtga cagctcccat
     9241 aatcaacagc atgatccacg aaatagcact agcatacccc atagaattaa aagaaaaagc
     9301 ttggttatac acataaagag cataaaagtt cgtggcatca ttcggaccac cttgggtaat
     9361 gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc tgcataacca agttatacaa
     9421 aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac gtctgccatt tgttcgctcc
     9481 atcgatctct gccgcttcat aatacgagcc tgggatctct ttgatacctg cagcaaagat
     9541 gatcattgcc gaaccgaatt gccaagcaga catcaaaaca agagggatca tgaccagttt
     9601 ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt tccaggaaaa tattcactaa
     9661 cccgttttta gaaaacaact cccgccaaac tagagcgata gaaatactgc caccgatcaa
     9721 actaggcaca taatacaaag aacgaaagaa agtcgttcct ttgatattcc tcgttaaaag
     9781 ataagcaatc aatagaccaa agatcagttt cacaggtaca gatgcaaata catagagaaa
     9841 agtgactcgg atggagttca aaaaacgggc atcactcagc atctttatat aattatccaa
     9901 gccgatccat gagactggat ttcccaaacg ataatcggtg aacgaataat aaagtgacaa
     9961 gatcatcggt atcactgtta tcaatagaaa tcctaggatg aatggagaag caaatacgta
    10021 accgaccaca ttgtcttgat ccaagaaccg ttttagtttt tcttttttca tgccttcctc
    10081 ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga ctctctgctg cttcttctgc
    10141 tgttacttct tcataaatga ctttttcaac taaaagattg tattggtctt gaatttccgg
    10201 attcaaagca ctatcaaaga cattcacttc gccgttgtcc atatcggaaa tcatttctaa
    10261 ataatcaaat gtcgcttgca aagttgcatc atctgcacga gcgagctgtt ctcgaatatt
    10321 cgacatgatt ggtacaccac gttctccttt taaaatttca ttggcttctt cactgttttg
    10381 aaagaaatca ataaacttag cagcttcttc tttatgggct gagctttttg ccatactcag
    10441 catttgcgaa gaacgtaaag ggataccaga ttctccatct gcttttctgc gaggtggatt
    10501 aatcaactga atctccatat cttcaggagc ccctgacaat atttctgtca actggttact
    10561 tgatagaaac accattgccg cttctctatc cctaaccgcc tgaaaagaca gatccgtcgt
    10621 acttgcagct ccaccacctg gttctggatg ggcccctttg tctaccagat ttttacgcag
    10681 ctggaaaaaa tctgttagat agtctggatt ttcaaatccc aagctgttag atagatcttc
    10741 ccgattataa aaattcaatc ctttctcaac ttgtggtatt tgaataatac agccagctat
    10801 aaaatcttcg aatcttgaaa tagcatagat tcctaattct tcagtgattt gatagctgat
    10861 ttgttcaaat tcatcccatg tccaatttga agctggttca gggatattta attgttcaaa
    10921 tatattttta ttgtaagcaa tcgcaaacga attcaccccg ttagacagtc caactaattg
    10981 cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca ttagaaatat caattatccc
    11041 ttgctccaca tattcatcta atggttcaat ctgattgatg tattgaggaa attgattgtt
    11101 cagttggaaa atgtcccaga tatcatctga tgctgctagt gtacctaatt ttgtgtaata
    11161 tccttcataa tcataaaatt cataatcgat cgtcacatgg ggattttttt cttcataaag
    11221 ctcgattact tttactgttg catcgtgtcg gacttgcgaa ccccaccaag ccatgttcag
    11281 cgtgattttt ttgtcatctt cttgcccaca agccgtcaag aagaataaca taacacttaa
    11341 agctaagatg attctcattc ttttcttcat tctctgcacc tctacttcca ttgttatttt
    11401 tcaatatact gtatttttat acagaacata tttattaaac gatctttttc aaggggaata
    11461 aaaaccaaga cttgccttat cgttctgcct aggatgaaca actcaatact ttcaagaaaa
    11521 attagatagt gctgattctt ctttcatcgc taaaaacact tcttgcttca atgatgggag
    11581 gataattacg caaagaaaaa aggatatcag acaactgccc aatatccttg acatagctaa
    11641 ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca gaaaaataac tagtaattct
    11701 gtatgaaatt aggtacaata tacacgtcga tgcacaagca ttgactatgg caacttgatt
    11761 ggtcagttcc cgctgacttt tcaagccccg acaatttagc tgctactaaa ttgtcgggtg
    11821 ccttttttag tttttattcg tttttcattc tatagaaagc gcttcaataa atcaagacat
    11881 ttttttgtta gaatgctaat aataacaaaa ggttttaact cttcttatgt acattacagg
    11941 taaaaaaaca aaaaaatttc tctttttttt gaattctgaa caaatagtac tggagtacaa
    12001 aagacgagga agagccacta tctgctcttc ctcgtctttt gattctcacc tgttattcat
    12061 gattcttttt ctcagtttcc cgatattttt ccattttacg actaaaaagc tctccgctac
    12121 ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat ggtctcagca atactttctt
    12181 ccgttggacc acctgtcgca atgataggaa gttcaggata ttttttacga aaatggcgta
    12241 ctgcttctgc agtttcttta cctgcactga tattgataat cgaaatacca gctgctaact
    12301 tttcatctat ttctgtatat ttcgacgtta ccgtactgat aactggaata tcaactactc
    12361 gacaaacttc ttttactgtc tctaaaggcg taggaccgtt caaaacgacc cctaacgaac
    12421 cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc accttgtgtc aaaccaccac
    12481 ctacaccgga gaaaactgga atgtcagctg cctcaataat gcttttagta atcgctggat
    12541 gaggtgtaaa aggatacaca gcaattactg cattcgcatt tgtattacgt atgatcgcaa
    12601 tatccgtagt aaagatgatc gaacggatca attttccaaa tatcttgatc ccgcttgctt
    12661 ctaaaatgat ttccggtact cttacgatat cttttctcaa ttccgtcatg atttctggcg
    12721 taaattgttc tgccatcagc cacttctcct ttgctatctc cggtcatact gattcaattg
    12781 atacgtctcg atgacctgga tgattttttg cgcataaccg gggtctgtcg cataacctgc
    12841 atcttgcaat gcttgtgcgg cttcctgata gttggtggcc gtaatcactt tttcatacag
    12901 cgcaggattc caatctactc cttggacgaa cagttgtgta tggtcatcca tggactgttc
    12961 ccatgaatca tacactttga agtctccttg gatagttatc cattcttcgt taacaaattc
    13021 ctttgtttcc aggcttactt tcttctgatc cccataagcc ttgatcccga aaagattatt
    13081 gtatttgcta gccaaggtac tttttcccca atttgattct aatatagctt gtcctaaaat
    13141 gatactagga agcactccat aaccatcttg tagttcttgg gcatgcggaa caagccgttc
    13201 aataaattgc tgatgggatt gcgcttcatt ttcttgctga ttatcagtaa aaggagaaga
    13261 cagattcttc aaggaaaaaa caaaggctaa gagtatcact gctacaccgg caaccactgc
    13321 tggccaacgg atattcttgc gcttttgctt tttccacttt tttttcgcca tgatcaattc
    13381 ctacttttca tttaattcat tcagatattc ttctagcgtc aattggtgtt tggtgatata
    13441 ctcagcgttt tctttaccga cataacgtaa gtgccaaggt tcatatgtga tcttagttat
    13501 atcttcgtgt ccttttggat aacgaacgat aaatccatac tgcgcagcat tatctgcgat
    13561 ccatttagct ccaggctgat ctccgtaact tgcatccaat acagtagaag gataactgtt
    13621 gtaccagttt tgatcgacga catcgactgc cagacctgtg tggtgttcgc tgtatcctgg
    13681 ctccgtaatc gtttctttag taatctttgt cgcctcctct tctgatacac cttgacgact
    13741 catcacatct tggacatttt gttcgaaaac ttgttgttgg gatgatacag accggtaagc
    13801 tgagaccatc actaatggaa atccagctgc ttcagcagcc ttggcaaatg cttcatattc
    13861 tggagcaatg cgtttgtcga tcaagtatcc gttacttaat gatgccaatt gagtagactc
    13921 atcaatttct ttttctaatt tgtgatctgg accaaccaaa actagcttcc aatcgtctgt
    13981 tttaacatct ggcaattgtt tggacgtacg atcttttgtt tctttgtcct ttaccttgct
    14041 gctggttgtt tgactgttct tttctttact agcagttgtt ttggtgcttg ttcgttcgac
    14101 tgcttgattg ccggcttgaa catgcgaatc atcatcgcta aaaataacat agcccattgc
    14161 tgcgatcata atcacaactg ctccaaatcc taaaaattta ttcacttgaa aaatcctttc
    14221 gttgttcttc acgttcccca ttcgcctcat gaacgtaagt ttcttccaga ttttcattca
    14281 cccaatcgag aagttcgatt ttttctcctt cgatctgttt ttgaacatct gctggcagac
    14341 gaaaattcaa aatatcatca tagccccatt catcatattg aatacttact ttcaagtcaa
    14401 ggtatctacc agcctgctga ttatttttca aagggatcag ctcgactttt gctgctttgc
    14461 tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt gtactctgca acggcaacgt
    14521 ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt ttgcgttagc atccattcat
    14581 aatcagtgaa aagatttttt actttctgca tcgcttcaga tggcagattt ttctcgccat
    14641 ctttccattt ttgccattgt tctggactga tcagcaagta ttcatctaaa aaattttttt
    14701 cagtttcaaa ctgttctaat aatacactca taataagatc aatatatact tgatgcacgt
    14761 ttatcccctc cttttaacta ttctacttaa gttttgatca aaaagcacct cttaaatcgc
    14821 tgctttcatt ttttttaatg tttcaagtcc tctcctcttt attcttcgtg atttttgtta
    14881 taataataga cagacaagaa actcaatcca actatgaagg agcgttttca gatgaaaaaa
    14941 tatcaatggg gaatcgttgg tcttggtacc atcgcacatg agtttgccga aagcttcaat
    15001 caagagacga gcgaactaac agcagttgct tccagaactt cagaaaaagc ggaaaacttc
    15061 gctcaccgtt acaatattcc aaaagcttac ggaagctatc aagaaatgtt agacgatgct
    15121 gaaatcgaca tcgtgtacat tgctgtacca aatagacagc atatcgacca tatcttggca
    15181 gcattggaag caggcaaaca cgttctatgt gaaaaagcca tcacgatgaa taaaaaagaa
    15241 ctggctgacg ctatgcgctt agcagaagaa aagaatttga tcttagcaga agcgatgaca
    15301 atcttcaaca tgccacttta tcagcagtta cgctcaataa tggatacagg aaaactgggg
    15361 gctttaaaaa tgatccaagc acctttcggc agttacaaag aaccagaccc taaaaaccgg
    15421 ttcttcaatc cagaattagc tggaggagct ctgttggata tcggtacgta tgctgtatct
    15481 ttcgctcgtt tctttttgag ctctcaacct gaagtagttg cctctacaat ggttcctttt
    15541 gaaacaggcg tggacgaaca atccgtcact atcttgcgta acaaagaaaa tgaacttgct
    15601 gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg gtatcgttgc ctttgagaac
    15661 ggttatgtga cgattgctga ttacccgcgt gctgatcgtg cggagatctt atttacagat
    15721 ggtacaaagg aattcatcga aagcgggaat acatctgaag caatgaatta cgaaataaag
    15781 aatatggtac acatgattga aggtgacttg cccaatcgct ctctcttctt gacaaaagat
    15841 gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa atatgtaatt ttaaaaggtt
    15901 gtgaaaaagc tgttctgcat caagtaataa gaacagccaa acaaaaatag ctcctcctat
    15961 ttttcgtttg gctggactta ttaccgcaga ggaaagcttt tgaacaccgt ttaagaaaga
    16021 ggttgtgagc ctaccgttca gctccgagtc acaaggagca atttcaaaaa acagcttctc
    16081 atgtttgttg aatattgtga cttgtgaccg aggagttgac aggagaacac cgtttaaaga
    16141 agaggttgtg aaatcagctt tttgacctga atattagctg aattcacaac ctctattttt
    16201 ggattttttg ttgggagaat tctgccttga tttcaataaa acttttaaaa gactgcaaaa
    16261 actggaacaa ttctgctcga tcctcgaatt cagggcgact agtcggcaga ggcatttgcc
    16321 gatacaatac ataaacttct tcaatacgtg ttaaaatttg tctgccatca tttgactctg
    16381 aaaatgtttc tgcagtataa atcaataaac catagatatg ttttccataa taagctggtt
    16441 gctggattct ctctagattt tccagcatgt ctttaatcac attggcctgt gctcttctca
    16501 tagagaagta agtttcgtag tatagcggct gtctgagcca gtagttttct tgatgttctc
    16561 tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg ttcgcatttg atctgcacat
    16621 cttctttatt ttctgataaa agaaattctg ccatttgtct caaaatattt cgaaattctt
    16681 tttcgatctg tatttggttt tccttcagct gcttcgtacg atctggcatg taaacatttg
    16741 caagcaaagc aaatactaat ccaatcgcca ttaatcccgc ttcattgccg atcagctgcc
    16801 aagacatgtt ttcctccact aaataatgag tgaccaaaac cgaatttact actatccctt
    16861 cggttaattt gaagcgagag gcaagcggga tgaacaggaa taaaaaaaga ctgaagccaa
    16921 aaatagtata cccaacagca aaaaaactga acagtgctgt tgccgtagct aatataaagg
    16981 ctgcgattcg attcatgccc gtacgcaatg tagagcgctt ggtatttcct acacttaata
    17041 atgcaatgat ccctgcagct ggccaataca acagcgataa gctacccgct gtgaccatcg
    17101 aaaagaagac actgatcact gttttgactg ttcgcattcc tatacgcatt tacttccccc
    17161 ttttttattt taattatact tgaataatga aaaactagct atcagacaaa agtttaggta
    17221 tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt tggccgtttt cgactaggta
    17281 tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat cttgtttttt catttgactc
    17341 aacgaggaat cccgatgatt gctgcattat ctgctgtttt ttctttgcgt caggacttga
    17401 ctacaaccgt ttcttttgga aagtctagga tcatcggcaa tacattaggt ggtacattag
    17461 ctatcattta tttttttgtc aaaagctatt ttcacaatga ttttttggta gaattgatct
    17521 tgcttcctat tctagttatt attgtgatcg tcgtgtcaga tggtattaac aataattccg
    17581 gtatcatttc cgcaatagct acgttgttgc tgattgcgtt gagcatccct caaggcgaat
    17641 ccgctttgta tgctatccaa cgtgtactag atacttttat agggacattc attgcaatcg
    17701 gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa ggaagaacta tcagaagatt
    17761 tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa tttacaagag atagaagcta
    17821 agatccaaaa acaaaagaaa aatagcgagg actaattttc ttttgcaata aaataaaaaa
    17881 atggagagaa gaactttaaa aagtcttcct ccatttttga ttgaacggtg aagtgtacta
    17941 ctttattctt ccttcatcga gttttgttct gcctcttttg gcgcttcata catatttact
    18001 tggctagtag tcccacgtgt cgacaaattc tctacgattt ccttcaagtc aagtcctgtt
    18061 gtcgctttta gtgtttcttg agtagaagaa agcaaatttg tggcataatt tgtcactcgg
    18121 ttcgctccac tggtttctcc tgaactgctt cctgtatcga caacagagat tttttcgata
    18181 tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta gcatatcgat aaccatactc
    18241 aagacagctg cttcgccata ttccttgaag gcattggcga tcttttgttt tgcttcggct
    18301 tctgcttttc cttttgctag tgctgcttcg gcttctgctt gtccagctaa acgtgtctta
    18361 ttggcttccg cttctgccag tgcttccaca cggaatcgtt ctgcttcggc ttcagtcact
    18421 tctttgactt tttgagcttg tgcttcttgt tctttagcgt accgatcggc atctgctttt
    18481 ttcttcactt ccgaatcata ttgcttttca cgacgtgtga tttctttttc ttccagttcg
    18541 atttgttttt gacgttctac gattttaatc tgcatttcct gttcgattac ttgttgttgt
    18601 gctcgggcac tttctaaatt gtaggcttgg tctgcttttg ctttagcaac atcttgttct
    18661 tgtttgtagg ttgctaattt cagttctttt tcttttaatg attctgcgat ctctgtttgt
    18721 cgctgtaatt cagctgcttg tgattctttt tctgcttgag cttttttgat acgcgtttct
    18781 tttagagctt ccgcttctgc tatatctgca tctcttttga cttgggcgat tcttggtttc
    18841 cctaaagagt caagataacc gtttttgtct ctgacctctt tgatagtgaa agaaacgatg
    18901 atcaacccca tttttgcaag gtctacagaa gcgacttctt gtacgctttg actgaactta
    18961 tcgcgatttt gatagatttc ttctacagtc atagaaccaa gaatcgagcg caagtgtcct
    19021 tctaatactt ctcttgcttc attttccagt tcttctcttg tcttgcctaa aaactgttct
    19081 gctgctgtag caatctcttc tacagatgaa ccgattttga tgatcgaagt accatcacac
    19141 atcaccggta cgccttgttc cgtatacact tcaggggtgg agacgtccaa tttactcgat
    19201 aatagactga tccgattcga acgttgaaaa actggcaaga cgaatgcgcc acctccacgc
    19261 acaattttga ttttgttatt gctttcatca atgtgcacat ttttgcttcc taaataactt
    19321 ccactgatga tcaacgcttc atccggtttt gctgtctggt acttcgctac aaagacgatc
    19381 agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga taaaaacaac tggtatttcc
    19441 atttaacaca cgctccttta agcttttatc atgctttcat aaggcatgac ataacagatt
    19501 ctttccttga tctcaatgat caatactttc gttccccgct cagctgtctg ttcttggggg
    19561 ctgtaaaaac aagctggacg actaatggaa ccggtaacac ttttcaactg aatttctccc
    19621 atacctcgta cagggatcgg tgtgatcaca acagctactt gtccttccag agttttttca
    19681 gaagacgaca tcgttgattc ggcatgtttc atcggcatca atatatagaa attaagcaaa
    19741 aataccaaca ctatcgatat gcctccacta gcaataaata aaaataaaga tgattgatct
    19801 gttaaacgct cacctagaaa accaaataag cttgtgaaag ttaaccaagg gataataagc
    19861 atcggatcaa gcgggccatc aaaatcgaat acatctccaa aaataatcaa taggactgcc
    19921 aatcctgcac aaatcagcaa tgtataccaa taaatcgtct caagtgcgat tccccctatc
    19981 atatacacgc ctccttccat ttctacgtct atcataccaa tgtttgaaaa ttctgtatag
    20041 cttcttttac cgactcagtg gtgtttttta taggaaggtt gtgtttatcg ttgacaattt
    20101 gttaaatttt tgttttcatg ttaaaatttt cacaataaat tctttcgagg tagcctatgt
    20161 ttcctttttt taagaaaaaa ccattaaagc tccagaacac gatcatcctt gtcgtttgtt
    20221 tctccacatt cgtttctttg attgtttctg ccatattgat caggaatttt attctttcga
    20281 tgaaccgatc tagtgtgtca gataaaatct ctagcattgc taaaatcgtt gccaatgata
    20341 aactcatcca aaaaaagctg cagactggtg acccgacaca tgcgattcaa ggaataacaa
    20401 atgaaatcat gattgcatcg aatttagatt ttatcgttgt catggaccct gaaggcattc
    20461 gatattctca cgcagaccca agttacatcg gtcttccttt ctcaagtcat gaagatgtga
    20521 aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga aggaatgtta gggttcggct
    20581 tccgctattt cgtccctatt tattatgatc aagagattat tggtgcagtt tgtgttggct
    20641 taacaaacaa cacgattgac aatcaagtca aagatggaca aaaaatgatt gttttttcat
    20701 tgattattgg acttagcgta ggcatcatag gcgccgtttt tcttgcccaa aaaatgaaaa
    20761 aagtactgct tggacttgaa ccacaagaaa tagcagaaca gttaagtgaa aaaacgatta
    20821 tcgaaaatga agtagatgaa ggaatcttag ccatctcttc tgataaaact atcctcttga
    20881 tcaatacacc tgcaaaaaac attttctacc aaacagagca gctattggat ttcgaagtag
    20941 gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa gagacgattg agcatcaaac
    21001 gaccgttcgg actcaatcca tgtatttaaa tgggatagaa actatttcta gcgttacacc
    21061 tctttttagc aatgaccgtt ttttaggagc agtcgcaact ttacgaaatc gatcagaagt
    21121 aatgaaatta atggaggagc ttggggggac gaaacaatat ataaactcca tgagagctca
    21181 aacccatgaa ttcatgaata aacttcatgt catttctggt ttgattgatt tgaagaagta
    21241 tgaagaagtc acccatttta tccagcagct aaatcaaaat tataaaaaag agattggcca
    21301 agtctcaagt ctgatcaaag tacctgcaat tgccggtttt ttattaggca aaataaacga
    21361 agccaaagaa caatctgttg aaatgctgtt agaaagcgat tcttttatcc ctaaaatgga
    21421 gatggatgac tccatccatt cacttttgca agtgttaggg aacttacttg ataatgcaaa
    21481 agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc cggattcatt taaactatga
    21541 cacagaagct gaagtattca tcgtgaccgt ctgcgatgac ggtgtcggga tatcagaaga
    21601 aatctcagaa aaaatattta cgctaggttt ttcgacgaag ggaatcaatc gtgggtttgg
    21661 attgaatctc atccattcaa tcgtcaaacg ccacggtgga tttttagaat tttccaaaca
    21721 aacaaatggc ggtacaatcg tctatctaga attaccaaaa aaggaagatc aaaatgatga
    21781 caaatgtcct gataatcgaa gatgatccaa tggttgcaga attgaatcgt acttttgtgg
    21841 aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa tgtagaagaa ggaaaaaaat
    21901 atttcagaca aaaaaacgca gatctgctgc tgcttgatgt atacatgcct ggcgaaagtg
    21961 gtatccattt tttatcttgg cttagacagc aaggaaatga tatcccagtg attttgatca
    22021 ctgctgcaga tgatatcgaa accataaaga atgtgatccg ctacggtgcc ttagattatt
    22081 tgatcaaacc gttcactttg caacgttttc agctggcgat cgaaaagtat cttgctttct
    22141 ctcagacaat ccaaacgact gaaaaagccg atcagaaatt gatcgatcaa gtattcaacc
    22201 acaccactcc cattcacgaa ataccatcat tcatcgattt gcctaaaggt ctgtcaaaac
    22261 tgactttaaa aaaaataagt gcatttattc taaaagaaga tactattttt tcaacaaaag
    22321 aactggctca aaaaagctca ctgtcacgta tatccgtcaa gaaatatttg aattttttag
    22381 tagaacaaga tatattgaac gaaagaatca aatatttgga aatcggccgg ccgctaacct
    22441 gttatgaatt gacaaagaat gggcggaaaa agctcacaga ttactgttga gtcatcgaat
    22501 tatcccatac aaaatagtcc aagtatacag aagtaacaac tgtttacttg gactattttt
    22561 aaacatttta ttgacgaata tccatgtttt cttatcttaa ttgacgggct ttaggaagag
    22621 atacctcatt tttcgttttt ccagtttcta ttatttcacg aaagttctcg aaacttgtcg
    22681 aaatcatatt tgataatgcc tcatctgtat ttgaacctac atgcggggtt acaagaactc
    22741 tagggtacat ttctactaat tgctggatag cagggtctgg aatcgtctcc catggcttga
    22801 aggatcggaa aaaaacctct gtttcatttg caaaaacatc ggttgcaaaa ccagctaaat
    22861 gattgctttt caaagctctt aaaatagctt cattatcttg aagctctcca cgggctgtat
    22921 taatcaagat gctacctttt ttcatttgag ctaaaaatcc ttcattgatc atttgatcgt
    22981 tttcacctgg gatatgaggt acatgaatac tgacaatatc gcttttggct aataactctt
    23041 caagcgagtg gaacgtcaat acttttttag cagcttctgt ctgatgaata tcgtagccga
    23101 tcactttggc tcctagtccc ttaaacaaaa cagcttccgt caaccctatc ctgcctgttc
    23161 ctatgatccc tactgtgcaa tttctgattt cacgactaaa catttgctca tccactcgaa
    23221 aatctttgaa agatgttcgg atcgtcgtat acgcagtatt tctcaaaagc atcatggcaa
    23281 aagtcaaaga taactctgct atcgcatttg gcgaatacga tggtacacgg gctactgtca
    23341 taccaaaatc agctgccgct tgtagatcga tatgattgac tcccacagtc cgtgtaaaaa
    23401 cataacgaat cccatattcc tgcatttttg caagattggt ctcatctgct atacaatttc
    23461 ccctcaacaa tacagcatca tggtcctttg ctgacacgat attgtcatgt gtcaacagtt
    23521 cttcataaag actgagatca taatggtaaa tattcaattt ttcaaaaaaa tctatctcgt
    23581 tcggacgaac accgtaacat gcgattctca tactttcttc ctccatttct tatgaaaata
    23641 aacctatgcc atgaatcatt tgtaatacaa cgatccatac aggtgtcagt accacagcaa
    23701 aaatagttga cagtaaggat gcattggagg ctaaaagtgc ctccctatca aaactgatag
    23761 cataggctgc tgccactgtt gcagctggtg tagccatcat gataacgatt gttgccagtg
    23821 cttcaaaatt gactggtaga atattcgttg ccgtcaaaac tgctagaatc acgatattca
    23881 atgcaggtac aaataccact ttgactagac tgtaatacca tgaagttttt tcagatgcag
    23941 cttttttgaa gcttacttca cctaatgttg cgccgattgc tagccacgcc aatggtgaac
    24001 ttaaaccagc taaataatcc atcggctgaa atagccacgg tgcagtctgg tcaattcgca
    24061 aaaacgctac ttggcttatc tctccttctg cacctgtaac tgacacttgt ggcagatacc
    24121 cttggaacag ccaaatgaaa aggccggcaa aagtagcgat aatgattgga ttgacgaaca
    24181 tcgctttgat atttttagct tccatcttca agccactcat ttttatgtat ccataagaat
    24241 acaaaaagat tcggtagcca atattgaaaa ttgaggaata catcacgccg acaggaccat
    24301 aaatcgcact gacgataggc gttccgaaaa aggtcgtaga accaaagatc gtcaggacac
    24361 gcaacgttgt ttgcttatcc cctttatagc ttaagaaaaa aggttttgaa actagaataa
    24421 gaataatata aataactaat ccccaaatca agacattgat tccttgcatc agtaattctg
    24481 tattgatatc ttgcatgaaa gctcgaaatg ccagtgctgg caaagccact gttaagacga
    24541 ctttcgatag aatttttcca acagattcag aaaaaatccc cttttttcta caaacaaacc
    24601 ctaataatat aataaaaact gttgaagtga ttgcactgac aatcgacata tctgtcaaag
    24661 tcgatcgaaa aatgtcaaat aagttcatct ttctcctcct aattatgttc atttattcac
    24721 aataaaaaaa gcttttattt tgctaatacg tatt
//
LOCUS       DAFGZE010000039        24620 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.040, whole genome shotgun sequence.
ACCESSION   DAFGZE010000039 DAFGZE010000000
VERSION     DAFGZE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.040"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            92..640
                     /gene="aac(6')"
                     /locus_tag="LTU54_001713"
     CDS             92..640
                     /gene="aac(6')"
                     /locus_tag="LTU54_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL8296428.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1186..2847
                     /locus_tag="LTU54_001714"
     CDS             1186..2847
                     /locus_tag="LTU54_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8296429.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2917..3264)
                     /locus_tag="LTU54_001715"
     CDS             complement(2917..3264)
                     /locus_tag="LTU54_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HBL8296430.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            3348..4289
                     /locus_tag="LTU54_001716"
     CDS             3348..4289
                     /locus_tag="LTU54_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296431.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            4290..5333
                     /locus_tag="LTU54_001717"
     CDS             4290..5333
                     /locus_tag="LTU54_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296432.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            5339..6409
                     /locus_tag="LTU54_001718"
     CDS             5339..6409
                     /locus_tag="LTU54_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296433.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            6399..7352
                     /locus_tag="LTU54_001719"
     CDS             6399..7352
                     /locus_tag="LTU54_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8296434.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            7364..8374
                     /locus_tag="LTU54_001720"
     CDS             7364..8374
                     /locus_tag="LTU54_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8296435.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(8659..9480)
                     /locus_tag="LTU54_001721"
     CDS             complement(8659..9480)
                     /locus_tag="LTU54_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBL8296436.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(9577..9855)
                     /locus_tag="LTU54_001722"
     CDS             complement(9577..9855)
                     /locus_tag="LTU54_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296437.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            10123..12132
                     /gene="metG"
                     /locus_tag="LTU54_001723"
     CDS             10123..12132
                     /gene="metG"
                     /locus_tag="LTU54_001723"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBL8296438.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(12611..13198)
                     /locus_tag="LTU54_001724"
     CDS             complement(12611..13198)
                     /locus_tag="LTU54_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296439.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            13373..14695
                     /locus_tag="LTU54_001725"
     CDS             13373..14695
                     /locus_tag="LTU54_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296440.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            14850..15626
                     /locus_tag="LTU54_001726"
     CDS             14850..15626
                     /locus_tag="LTU54_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL8296441.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            15619..16188
                     /gene="rnmV"
                     /locus_tag="LTU54_001727"
     CDS             15619..16188
                     /gene="rnmV"
                     /locus_tag="LTU54_001727"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBL8296442.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            16181..17065
                     /gene="rsmA"
                     /locus_tag="LTU54_001728"
     CDS             16181..17065
                     /gene="rsmA"
                     /locus_tag="LTU54_001728"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBL8296443.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            17516..18145
                     /locus_tag="LTU54_001729"
     CDS             17516..18145
                     /locus_tag="LTU54_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296444.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(18269..19378)
                     /gene="ugpC"
                     /locus_tag="LTU54_001730"
     CDS             complement(18269..19378)
                     /gene="ugpC"
                     /locus_tag="LTU54_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBL8296445.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            19587..20009
                     /gene="mgsA"
                     /locus_tag="LTU54_001731"
     CDS             19587..20009
                     /gene="mgsA"
                     /locus_tag="LTU54_001731"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBL8296446.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            20332..20868
                     /locus_tag="LTU54_001732"
     CDS             20332..20868
                     /locus_tag="LTU54_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HBL8296447.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20908..22671)
                     /locus_tag="LTU54_001733"
     CDS             complement(20908..22671)
                     /locus_tag="LTU54_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296448.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(22674..24389)
                     /locus_tag="LTU54_001734"
     CDS             complement(22674..24389)
                     /locus_tag="LTU54_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296449.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7964 a         4471 c         5005 g         7180 t
ORIGIN      
        1 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
       61 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
      121 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
      181 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
      241 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
      301 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
      361 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
      421 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
      481 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
      541 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
      601 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
      661 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
      721 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa
      781 aagtcgctta gtttcgagta gtaagaaaaa ttttgtgaaa atggctttct acatttttgc
      841 ataaatttgg cttaataccg agaaactggc ttttgaacac cgtttattca gataagggac
      901 tgtgacaaga cttttgtcac agtttctttt tgtttattct tttttatctt tgtatggcaa
      961 taatagacaa tataacgaaa attatttaat ggcttttgga agaccatgga aaaccttgta
     1021 caaagctatt ttttctgaaa aattccttga aacctcacga aaaaatcatt attttatgaa
     1081 aaagacaaaa aaaagtgttg attttttaat aattattcat taaactctta aacaacttca
     1141 gaaaatatta attattctga aaattccaat aaaggggtac gtaaaatgaa aaagaaattt
     1201 acatttggtt tggttgcggt atgcggcctt gtacttgctg gatgttatgg cggcagttca
     1261 gccacaagca gtagttcagc aaaaagcggt tcagcagatg gaggaggcgt atttaactta
     1321 gtcgtgcctc aagaaatgcc tacagctgac ttatcagttg caacagatac aatcagtttt
     1381 acagcattga ataacgttta tgaaggaatt taccgtcttg atgaagacag caaaccacag
     1441 ccggcaggag ctagcgagtt ggcagaagtc agtgaagacg gattaactta caaaatcaaa
     1501 ttacgagaag acgcaaaatg gtcaaatggt gatcctgtta cagcagcgga ttacgtttat
     1561 ggctggcaac gaacagtaga cccagctaca gcttctgaat atgcttacct gtatgcgcca
     1621 gttgaaaatg cagaagacat tacagctgga aagaaagata aatcagaact agggatcaaa
     1681 gcagttggtg actatgaatt agaaatcaaa ttgacgaaac aaacaccata tttccaatat
     1741 ctgcttgctt tcccatcatt tttcccacaa aaacaatccg tagtggaaga aaatggggat
     1801 gcctatgcat ctgcaagtga taaagctgtt tataatggac cattcacatt agaagatttt
     1861 gatggaccag gaacagatac agaatggact tataagaaaa atgatcatta ctgggacaaa
     1921 gatacagtaa aactttctga aatcaaagtc agtgtagtaa aagaatcttc tactgcattg
     1981 aacttattca aagatggcca agcagatgac gtgatcttgt ctggtgagtt agcacagcaa
     2041 aacgcaaacg atccagctta tacttctgtt aaagaagcgc ggacaagcta tatcgaattc
     2101 aatcaacgtg aaaagaattc accattcaac aatgtgaacc taagaaaagc tatttcttat
     2161 tctatcgatc gtgaagcttt agtaaaacaa gtactaggtg acggatcagt tgtctctaca
     2221 ggattgatcc catcagatat ggtaaaaagt ccaacaacga acgaagactt tgcaaaagaa
     2281 gctggaaaat tagtttctta tgacaaagat aaagcaaaag aatattggga aaaagcgaaa
     2341 aaagaattgg gcgttgattc tttagaattt gatttgatgg cttctgatga tgattcatcg
     2401 aaaaaagtaa tcgaatatgt tcaaaactca atccaagaaa acttggatgg cgtaacagtc
     2461 aaaccaacac ctgttccatt ctctgtacgt cttgaccgct caacttcggg tgattttgac
     2521 gcagtactag gcggatgggc tgctgactat gcagacccaa gcagctttac tgatttgttt
     2581 gtcacaggta attcatacaa ccgtggtcgc tggagcaatg cagaatatga cgaagcagtt
     2641 aaagcagctt catcaacaga cgctggcgat gaacaagccc gttgggatga tttccaaaaa
     2701 gcaatcaaga tcatctctga tgatatgggt gtcgttccgg tgtaccaaaa agcagaaggt
     2761 cacctagtga atgaaaaaat caaaggtatc gttcaccatg cggcaggcgc ttcatgggat
     2821 tacaaatgga catacgttga agaataatcg ttaataccgt gagcatgaga aaaaacgagg
     2881 ctgcggcaaa tgtcgcaacc tcgttctcat attcacttat cctaacaaag aaaaaagcat
     2941 gaaaatagca gaaagagcaa ttagtattcc ggcaatgatc agccaaaagc tgtacatacc
     3001 gtagctggca gacgagagcg aggaaaattc tggttttttc tttcggttcc ttgttcgtgc
     3061 aagatggacc gatcgctgaa aatgatcgaa gaaacccgaa gaaaagaccc ataaaaaacc
     3121 gccgataatc agaaacggca atgaccataa gaatagatca ttagataatt gaatgagtgt
     3181 aaaggaatgg ttgatgagat tttttattaa aataaccaga atcagtagaa aagctactgc
     3241 gtaggggata gatttttttt tcatttgatt gctcctttat ttttggttca ttatctaata
     3301 atgtagcgaa acatctgtca gaagtcaaaa gctaggggga aacattagtg aatagttata
     3361 tcaaatatgt attaaaacgt gtctttttca tgatcatcac gctttggtta attgcaacaa
     3421 tcaccttttt cttgatgcaa ttgctgccgg gcacgccata tacgaatcag gaacgtttga
     3481 gtcctgaaac aatcgagatg ctaaataaac aagtcggttt ggataaacca gttatcgtac
     3541 aatatgggat ttatctttct aatttgcttc aaggagattt tggaatttcc ttccaattta
     3601 aaaaccagcc agtcgcacat ttgttagctg gaagaatcgg accatccttg cagttagggt
     3661 tacaggcaat tatctttgga acagtcctag ggacgatttt aggaacgatc tctgccatga
     3721 agcaaaatac atgggcagat acatcttcta cactcgtagc gatattaggt cgttccattc
     3781 caaactttgt atttgctgtt ttattacaat atatctttgc aatcaaatta caagttttgc
     3841 caatcgcaaa atgggatggc tttgtgtaca caatcttacc aacaattgcg ttggccatgt
     3901 cgccgcttgc tgactctgct cggtttatcc gtacggaaat ggtggaagta ctgcatagtg
     3961 attatgtgga attagcaaaa gcaaaaggat tgagccgttg ggaaatcgcc tttaaacatg
     4021 gtcttcgaaa tagtttgatt ccattgatga ctttattagg accgttagct gtagcattga
     4081 tgaccggatc actggtggta gaaaatatct ttgctattcc tggtatcggg gaacaattcg
     4141 ttaagtcaat cacaacaaat gattatccta cgatcatggc tgtaacgatc ctttactcat
     4201 ttatgctgat ttttgttatt ttgatcgtgg atctattgta cggtctagtt gatccaagaa
     4261 ttcgtgtttc agaagggagc cgaggataaa tggaaatgat accaaagcgt tatgaaacag
     4321 ttgcagatat ccctgcggat gaatttcttc cattacaaaa aaataccgaa gaagaacgtg
     4381 aacagattac tgcaccttct ttgaatttta tccaagattc atggcgccgt ttaaagaaaa
     4441 acaaagctgc cgttatttca atggttttac tattggtcat cattttgatc tctgtgatca
     4501 caatctttgt ttctcctcat gatccaactg cccaaaacgt ggcttatatc aatttgcctc
     4561 ctcgtattcc gggaatcaat atcgatggat tgaatggaaa agcaatggtt ggtggtgaac
     4621 ttgttgataa atatgctcaa gcaaacgtcc cttcaaacgt aaatttcttt ttaggaacag
     4681 acggtttggg acgtgatgta ttaagccgct tatttatggg aatgagaatc tcgttgctga
     4741 tcgcatttat tgctgcatta ctagatgtaa cgattggtgt agcttacggg ttggtttctg
     4801 gtttgttagg cggacgtgtc gataacgtga tgcaacgttt tttggaaatc ttatcaggaa
     4861 ttccaaacct cgttgtcatg attttgatgt tggtcgtttt tgaaccagga attttttcaa
     4921 tcgtggctgc tatggccatc acaaactgga taccaatggc acgtatcgtt cgtgcacaaa
     4981 caatgaaatt aaaagatcaa gaatatgttt tagctggatt gacattaggt gaatcaagaa
     5041 tgaaaattgc attcaaacat attttgccga atatctctag tgtcattatt attcaaatga
     5101 tgtttagtat tccatctgct attttcttcg aagccttttt aagctttatc ggtttaggtc
     5161 ttacgcctcc gtcagcttca ttaggaacga tgttgagcga agggtacaag acattcctgt
     5221 atctgcctta tctattatgg attccggcag taactttatc ggttatcatg attggcttta
     5281 acttacttgc tgatggattg cgtgatgcct ttgatcctaa aatgaaagag tgaagacgat
     5341 gacgaaaatt ttagaagtga aagacttaga aatctctttt gatacgtatg ctggaaaggt
     5401 acgagcgatt cgcggtgtca attttcattt gaataaagga gaaacattgg cgatcgttgg
     5461 ggaatctgga agtggaaaat cagtgacgac cagaagcatc atgcgcttgc tgtcaagcaa
     5521 cgccaatata gatgctggtc aaatcctctt taaaggtaac gatatcgttg gaaaatcaga
     5581 aagagagatg caaaaaatcc gtggtaaaga aatagcgatg attttccaag atccaatgac
     5641 ctctttagat ccgacgatgc ccattgggaa gcaagtcgct gagtccctta gaaagcataa
     5701 taaagtttca aaaaaagagg gacaaaaagc tgcattagaa ttgcttaaat tagttggaat
     5761 acccgaagct gagaaacgta tcaacagtta tccgcatcag ttctcaggag gtcagcgtca
     5821 acggatcgtg atcgcgattg cactggtatg ttatccggaa atcctgattg ctgatgaacc
     5881 tacaacagcg cttgatgtga ctattcaagc acagattttg gagctgctga aagaaattca
     5941 gacaaaaatc gatacatcaa ttatctttat cactcatgac cttggtgtag tagcgaatgt
     6001 cgctgatcgt gtcgctgtca tgtatggagg aagaatcgtt gaagttggaa catcggaaga
     6061 aattttctac aacccgcaac atccctatac ttggggactg cttggttcaa tgccgacatt
     6121 agatagtgcg aacgatcgat tgtatgctat tcctggttct ccaccggatt tattgaaccc
     6181 gccaaaagga gacgcattct atccaagaaa tgaattcgcg atgaaaatcg atgcagaaca
     6241 agaaccacca ttttttgaac tatcaaaaac acacaaggca gcaacttggc tacttgctcc
     6301 gcaggcaccc aaagtgacac cgccagaaga aatccaacgt cgctgggcta tctttaaaga
     6361 gaaacaaaaa catacttacg gtcaaggagg aattgctgat gccaaataaa ctacttgaag
     6421 ttaaaggatt gaaacagtat ttcaatgtag gaaaaaagaa tgaagtacat gctgtaaatg
     6481 acatcagctt tcacatttat gaaggagaaa cattcggttt agttggagaa tctggaagcg
     6541 gaaaatccac tactggccgt acgatcattc gcttgaatga acctactgat ggtgagatat
     6601 tattcgacgg tcaagatata acgaagatca aagataaaaa aggtttgacg aaattccggc
     6661 atgatgttca gatgatcttt caagatccat acgcttcgtt gaatcctcgt atgaaagtca
     6721 gagatatcat tgccgaagga attgatgtta atggactagc taaatcatct gaagagcgtg
     6781 caaaaaaagt cgatgatcta ttgagaactg ttggattgaa tccaagccac ggtactcgtt
     6841 atcctcatga attttcaggt gggcaacgcc agcgtattgg tattgcacga gcattggcag
     6901 taaaaccgcg ttttatcatt tgtgatgaac caatttctgc actagatgtg tcgatccaag
     6961 cccaagtggt caatttgttg caagatctcc aaaaagaaca tcaattgacc tatctattta
     7021 ttgcacatga tttgtcgatg gttaaacata ttagtgaccg aatcggtgta atgcataacg
     7081 gcttgttatt agagatggga acaagtgatg aaatctataa tcacggtgtt catccttata
     7141 cagaaagttt gttatctgct attcctctac cagatccaga ccatgagaga aaacgcagaa
     7201 gaatcaaata tcaacctgaa ccagatgacg gacaagtacg tcaacttcgt gaaatcgcac
     7261 cagaacattt tgtctatgca actgagcagg aagtcccata ttacgcaaaa aaactaaaac
     7321 gacaaaagga atctcttctg gttgctaact aggagggaca tagatgctaa aagcttttaa
     7381 attccgtatg tatccgacag aagaacaaaa acagcaattg attcgtactt ttggatgtgc
     7441 tcgatttacc tataaccatt tgctgaaaaa gcggcagaaa agttggcaac aaacaggagt
     7501 agccaatttt tcgctcacac ccgctacatt aaaaaaagaa tacccattct taaaagaagt
     7561 agacagtctt gctttagcaa atgcacagct taatttggat cgggcttttc gcaattattt
     7621 caagggacgt gccagcttcc caaagcttaa aacgaaaaaa agtatgtggc aatcttatac
     7681 gaccaacaac caaacccgta cgatctatct ggaaaatggc tacttaaagt taccaaaaca
     7741 aaaagaacta atcaagataa atcagcatcg gccagttgaa ggatcgattc gctccgctac
     7801 gatttctgct cgatataatg aagaatttta tgtcgcacta ctttgtgatg tttcgccagt
     7861 aaaaaaagaa tctttagcta aatggatcgg gatcgcctat catccgaaga cattgatcca
     7921 aacatctcga cctctagaag taactttacc aaaatttcac cagacagaag aaaagctgca
     7981 acatgcacag cggaaattga atgtgaaagt ccgctccgct caccatagaa agatcaggtt
     8041 agatcaggca agcaattatc agaaacaaaa gagaaaagta atggatcttt atctgaaaca
     8101 aaagaaccag cgagaagatt atcttgaaca attatctggg aaattggtca aacaatatga
     8161 ctacttattt gtagaatctt ttccaaaaga agaggcacat gctgattttt ctattcacga
     8221 ctggcataaa ttaataacaa aattgcgtta taagtcccaa tggtataata aaaaatttct
     8281 tttgatcaat accgacggag cagaagaaag caattccgta agaaagagcc aagtagtaga
     8341 aaaaatgggc agacattcgg ttatcaaggg ataaaaaatg aatgaaactg ataggagcgt
     8401 tagggttagc cttgttaata aaaagtctta atgacttttg tttctaggaa tttttctttt
     8461 gaagaaaagc tcactaatta tgacaaacca ataaaacaat agcagaaaaa gttcgtacga
     8521 gctttttctg ctattgttaa ttttttattc agaaagttta cgaatacatc aaaattctga
     8581 aacaaaaaac gtaactcaca aagagttgtt tttgcgtttt ttcttgaatg attaggtctg
     8641 aatcttttgc tcacaacctt attcatggtg ccccggcttg agctgataga tttttgaagg
     8701 acggccgatt cctacaggac gttcaccgat ctcatcgaaa tattggagca tagctttctt
     8761 aaaattggaa tgatcgatcg ttttataatc gatccctaag aatttagcaa atacttttcg
     8821 agcttccgta atcgtgaaat ctttacctaa aacttgtaga acttgcggct cgtgttccat
     8881 tttattgact acacggttga atgctttcaa gatgatttga ctatggtcaa aagccaaagt
     8941 atctttgcca gaagactctc cagtgactaa atcaagcgag atagccactt ctccgctaga
     9001 taaatggatc ttgttactat gacgttctag tgtgaaccag cgtacttcct tggcatcatc
     9061 tccggcgatt agcggttctt ctccaataaa ggctaagtag ctgactgtga cgacccagcc
     9121 tcgcggatct cgattaggcg tactgaatgt atgcagttgc tcaatatttt cttttgaaat
     9181 aaccacacca gtttcttcct ttgtttctct taagacgctt tctccagttg actcatcctt
     9241 ttggacaaag ccaccaggca atgcccatga atttcgataa ggatgccctt ttctttggat
     9301 caacaaaact ttcagctgat cttcttcttt gttatagcac atcagtacga tatcgacagt
     9361 caaagagggc ttgtcatatt ctggacgttc ctgtttttga taccagttaa gaaattcttc
     9421 ttcacttgct ttatgttcat aatataattt ttcttcttct ttagaagcaa aacggttcat
     9481 ctgaccacct ccattgacag taaaagtact ttttacctta ttgtagcaga ctctgaaaaa
     9541 aaaccaagga tttacttgaa aaatctaggt tatcatttaa atatgaaaaa taaatggaag
     9601 tatgagattg aataaaaaga aaagcagata aaacggcaat actttatacc gctcttcacg
     9661 aatggcaaag aagccacaaa taacgatata tacgtcaatg ataaaacgca aaatccaagc
     9721 tgctgtcata aaactgttca aatgcccatg gaggttaaaa taaccataca tacgaatata
     9781 aaaatagctg aatattaaat gtgctaatga aagaaaagca atactaagtg taaaaaaaag
     9841 atgtttcttc tgcatgattg ttccagtccc ttactaagat agatagtctt tattatacca
     9901 cgtgtgaggg gagaaataaa aagccgtttt ttagtagata tgaaacaaaa caaaaacccg
     9961 catttattca acctattcat ccttttaaag gatagattga acgtagatag aagtcgttta
    10021 tccagtcttt ttcattttga aaaaaagcta acagcatgct ttttttatgg tatactagca
    10081 aggaaacgaa ttcgcatcat gcgataaagg aggaagagcc ccgtggcaga aaaggaaact
    10141 ttttatatca ctacgccaat ctattatcca agcggcaaac ttcatatcgg caactcgtat
    10201 actacgattg catgtgacgc aattgctcgt tataaacggt tgatgggctt tgatgtgttt
    10261 tatctcactg gagtggatga acatggtcaa aaaatcgaaa caaaagcaga agaactaggt
    10321 gtaaaaccgc aagaatatgt agataaaatg gcggctgatg tcaagaaatt atggaaaacg
    10381 cttgatatca gttatgataa attcattcgc acgacagatg attaccataa agcagctgtt
    10441 caaaaaatat ttgatcgttt attagaacag ggagatattt acttaggtga atatgaaggc
    10501 tggtactctg tttctgacga agaattcttt acagaaacac aacttgcaga agtgtacaaa
    10561 gatgacgaag gtaacgtgat cggtggtaaa gcgccaagtg gacacgaagt agaattggta
    10621 aaagaagaat catacttctt ccgcatgagc aaatacgcag accgtctatt ggaatactat
    10681 gaagaacatc ccgaattcat ccagccagaa tctcgtaaaa acgaaatgat caacaacttt
    10741 atcaaaccag gattagagga tcttgcagtt tcacgaacaa cttttacttg gggagtacca
    10801 gtcaagagtg atccgaaaca tgtggtgtat gtatggatcg atgcattatc caattatatt
    10861 actgcactag gatatggctc cgaagatgaa agtctattcg aaaaatactg gcctgcagat
    10921 gttcagatgg tcggtaaaga aatcgttcgc tttcatacga tctattggcc aatcatgctg
    10981 atggcccttg atttgcctct acctaaaaaa gtatttgggc atggctggct attgatgaaa
    11041 gacggaaaaa tgtctaaatc aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt
    11101 tatggtttag atgcactccg ttattatttg cttcgtgctg ttccgtttgg gtcagatggt
    11161 gtctttacgc cagaagattt tgtttcccgt gtcaattatg atctggcaaa tgatctaggg
    11221 aacttattga atcgaacgat tgctatgatc aacaaatatt gtgatggact cgttcctgac
    11281 tatgcctcac ttgtcactcc atttgatagt gaactgtcta cgacggctgc taacgttgtc
    11341 ggacgttacc atgatgcaat ggaaaaaatg gaattcaata cagcattggc tgaaatctgg
    11401 gtgctgattt ctcgtgcaaa caaatacatt gatgaaacag aaccatgggt attagcaaaa
    11461 gacgaagaaa agaaagctga attgaatagt gtcatgatcc atttagctga atctttgcgt
    11521 atcgcagcga tattacttca gccaatcatg acagaaacgc cagagaagat cttcaatcaa
    11581 ctaggattgg actctgaaac aatgaatctt gaaggattac actttggcga atttccttca
    11641 ggaacaaaag tagttgcaaa aggtacacca atcttcccac gtttagatat ggaagaagaa
    11701 gttgctttta ttcaagaaaa aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa
    11761 tgggatccag aagaaacaga acttgtttca acgaaagaaa aacaaatcaa atttgatgta
    11821 tttgaaaaag tagaactaaa agtagcagaa gtcatcaact gtcaaaaagt agaaggggca
    11881 gataagttgc tgcaattccg actagatgct ggagacagcc aagaccgtca aatcttgtca
    11941 ggaatcgctg aattttatcc tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta
    12001 gcaaatctga aaccacgaaa gatgcgtgga caaatcagtc aaggaatgat cctttctgca
    12061 gaagcaccag atggctcttt acaagtaatc gaagcaccaa aatccatgcc aaatggctca
    12121 gaaatcgcat agaaattcaa agggggtatg acactagttg tgtcataccc cctttttccg
    12181 aaatttgaga agcaattttc gtaaattcct tcttattact tgaagccaat catataatac
    12241 agaaacagag aactatccaa aataaatcct agaaaaagaa gtacgagctt cgctgatttt
    12301 tgttaattta gaataatact aataggaatc gtaaaaacag aaaaaaagca taatgataaa
    12361 gggtcgggca gattttaatt gattcatctg gtactctttt ttattgtcct tacattcata
    12421 aagtttgaat gcaatgactt tattacatct atatttatat ctaacagtag cgttgatgtg
    12481 ttaattataa gttctatcac tttatgttca attagtttgt gctcttatat gtgtgattga
    12541 aaatatagat aaaaaaacaa tcatcggctt tgacttcgat gattgttttc tttattttgt
    12601 aaaaatccta tcacttgatt ttatggtaac cttttatttt tgtcttcaaa cggcttggta
    12661 agagagcagt aagcagtgca aatgggatcg aaaagacata ccagaaaatc gtacgtccga
    12721 taccataatg ttgatagagc tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca
    12781 aggagccagc tataaaccaa tgattatccc aatgcatgac ttcactaagt gccatcactc
    12841 caaaaggata gaaataaaaa tagatcccga ctaaggtcat ccgccagttc tttgtaaatg
    12901 ttccgatatt caataaaaag aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc
    12961 caaccaaaaa ggaatacaac atcccccaca agatcatcac aggacctttg atcaatgcaa
    13021 cgattgcgat gaacaaagtc agcaatatta gtttttgtga taacgttgca acagtcatta
    13081 atccaaataa aattaatgtg aaccaatgcc aatgggactg ctgttgttct ttacgggaaa
    13141 aatcgacccg gttggataaa taatctgcta atttcggata agaacgctta aaatccataa
    13201 actgttcctc cttcgtggaa aaggtaggtt tagtatacga aaaataaaaa agaaatacat
    13261 ccgtcttaag agggagtttt tcactgaaag tggagaaaaa agaatggtaa attaaaatat
    13321 tctcagttat actgaaaatt ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg
    13381 caaactaaaa cgagaaatca atctgtttgg cgcattagct accgttatgg gaaccgtgat
    13441 cggtgcaggc gtcttcttca aaactgctgc tgttactgct agtactcagt ctacaagttt
    13501 gacattgcta gcttggcttt tgggtggttt cttaacgatc tgtgctggat tgacagtggc
    13561 tgaattagct acagcaatcc cggaaactgg tggagctgta aaatatattg aagctgcgta
    13621 tggaaaattg cccagctttt tattaggatg ggcgcaaagc ttgatttact ttcctgctaa
    13681 tattgctgca ttatcgatta tctttgcgac acaattgacg aacttattac aattatctac
    13741 cgattatctt ttgttgattg cagtgatcac cgctgtttct gtgacagggt tgaatttgtt
    13801 agggacaaaa gtcggtgctt ctgtacaatc agtgacgttg attgttaaat taataccaat
    13861 tgctgtgatt gtcatatggg gattattgac cccaggacaa ggaactgtcc agttatttcc
    13921 aatcgaagct ggaaaagatg ttacttttgt cgaaggattg agcagtgcgt tgttagcaac
    13981 gttgtttgct tatgacggct ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc
    14041 ggaaaaagat ttgcctaaag caattattct tggtttgagt tttgtaacag tcgtttatct
    14101 attgatcaat tttgtttttt tgaaaacttt gccgattgat catcttgccg gtaatttgaa
    14161 tgcagcttct gaagcatcag acgtcatttt tggaggaatc ggtggaaagc tggtcactat
    14221 tgggatcttg atctcggtat acggtgcatt gaacggttat acactaactg ggatccgcgt
    14281 gccttatgcg atggctttag aagacgagct acctttcagt aaacagctga caaagctttc
    14341 aaagaagttt acggttcctt atgttcctgc ggttttccag ttagcagtag cttgtattat
    14401 gatgagcctt ggttctttcg actttttaac agacatgttg atttttgtga tgtggctatt
    14461 cagtttactg atttttatcg gtgtctttat tttaagaaaa aaagcgccgg aattgccacg
    14521 tccatataaa gtaccacttt atccaatcgt tccgattatt gcgattctcg gtgctatttt
    14581 cattttaggt atgacgatga tgacgcaaac gaaattagca ttgatcggta taggtgttac
    14641 cttgataggt atcccagtct attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat
    14701 ttcaacaaca gacagaaaaa gtacgaacta ggtcctctag atcttcgtgc tttttctatt
    14761 tcaataaaac aagttcttct attttgattt ttgctacttc tgcaagtagt gcttatgtgc
    14821 taaagtaaga atgaaaagga gagataaaca tgatatttga ttctcatacc catttaaatg
    14881 ctgaacagtt caatgaagat attccagaaa cgattcagcg agcacaagaa atgggggtaa
    14941 cgaaaatggc ggtcgtgggc ttcgatacgc caacgattga aaagtctttg caattaagcc
    15001 atgactatcc aaatatatac agtattatcg gctggcatcc gacagaggct ggcagttata
    15061 cgaaagaaat cgaaaataaa ttacaagaac aattgacttt accaaaggtt gtagcattag
    15121 gggaaattgg cttggattat cattggatgg aagacccgaa agaagtccaa gataaagtat
    15181 ttcgcagaca gattgctatc gcaaaagaaa tgaacctacc aatcagtatt catactagag
    15241 aagcacttga agacacgtat caaatcctaa aagaagaaga tgttcgagat atcggtggaa
    15301 tcatgcatag tttcagtggt gattatgaat gggcaaaacg atttcttgac ttagggatgc
    15361 atatttcatt tagcggtgtc gtcactttca aaaaagcatt agacgtccaa gaggctgcag
    15421 caaatgtacc aatcgaacga ttacttgtgg aaacagatgc cccttatctg gcaccagttc
    15481 cttttagagg aaagcgaaat gaaccaggct atactcgtta tacagtagaa aagattgctg
    15541 aacttcggaa tcttccgaca gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat
    15601 tatttggtct aagcgaacat ggataagatg aagatccaag agattatagt cgttgaagga
    15661 aaagacgata caagaaggct gcgggaagta gttgatgcag atacgataga aacaataggg
    15721 tctgcaatca atgaagagat cctgatgcaa attgaacatg cccaagagac tcgtggagtg
    15781 atcgttttta ccgatcctga tttttcagga gaaaaaataa gaaaaatcat tatggaagct
    15841 gttcctcagg caaaacatgc ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga
    15901 agccttggtg tcgagcatgc aagtgatgct gctattttag aagcattgaa aaaggtcgtg
    15961 acgccagaca tttcccaaac ggctgtttct gaaatcacta gacaagacct tttagactat
    16021 ggtttaatag ctggggcaca agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc
    16081 atcggttaca cgaacggcaa acaattggaa aaacgtttga agatgtttcg tattacgccg
    16141 aaagaactcg cccaagcaat gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat
    16201 tgcgacaccc tctagaacaa aagagatttt attgaaacat ggtttttcat ttaaaaaaag
    16261 tttaggacaa aattttctga ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca
    16321 aatagatgaa catacgaacg tgatcgaagt tggaccagga atcggtgcat tgacggaaca
    16381 attagccaag catgcaaaac aagtcgtagc atttgagatc gatgatcgtt tgatccctgt
    16441 attggctgat acgatgcaac cctatgacaa tgtgactatt gtacatcaag atattttaaa
    16501 gaccgatttg tcaacagctg ttagagaaac atttcatgag gagcttccgt taaaagtcgt
    16561 agcgaattta ccttactata ttacgacacc gatcatgatg cattttcttg aatcagattt
    16621 ggttgtggat gaactagtcg tcatgatgca aaaagaagta gctgatcgta tctctgctga
    16681 accaggaacg aaagcttatg gttcattatc gattgctgtt caatattata tggaagccag
    16741 tttagcattc atcgtaccaa aaacagtatt tgtccctcag ccaaatgtag attcagcgat
    16801 tttgaaactg acaagaagag atataccggc agtcgaagtg acagacgaaa aagcattttt
    16861 ccgattgaca aaagcggcat ttcagcaaag aagaaaaacg ctttggaata acttgcagca
    16921 ttcgtatggc aaagatgacc agacaaaagc atggttagca aaaagcttgg aaacagctgg
    16981 aatcgatcca aaacgtcgtg gggagacttt gtcattacaa gaattcgccg cattaagcaa
    17041 tgcgatgtca gaaaatatgc agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg
    17101 tcctgcatca agtaataaga acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt
    17161 tgggcttatt accgcagatg caagcttttg aacaccgttt aagaaagagg ttgtgggcct
    17221 gccgtttaac tctaagtcac aaggaacaat ttcaaaaaac agcttctcat gtttttgagt
    17281 tagttggtct tattacttga tgcaagcttt tgaccactat tatgccgaac aaggagatgt
    17341 gacacaaatt ttgtcacatc tcctattttt gtttcttgat caaaaaattc ttatacttga
    17401 tactcacact cattttgtat ggatttagaa aagaatatgt gtcttgacag ttattagtaa
    17461 gtgagttata ctaagtcgac atttatgtgt caagacatat taggagagag aaaaaatgag
    17521 aaaaaattca gttcgaaaac taacgatttc tgctttattg attgggatgg gggtcattat
    17581 cccaatggtc atgcctaaaa tcatgattcc accagcttcc tttactttgg cgtctcatgt
    17641 gccattattt attgcgatgt tttttacacc aggagttgca gtggctgttg cgctaggtac
    17701 gacatttggt ttctttttaa caacgccggt tatcatcgca ttgcgagcat tgtcacatct
    17761 tgtgtttgcg ttgattgggg cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa
    17821 tggacagttt acttttgcta attggcgttt ccaagggttc aattttgtga tcggtgtgat
    17881 tcattcttta gcagaaatgc tagtagtcag catcttttac tttattggta atatgcctga
    17941 aacatattat tctcaaggat atttttatac agtattcatc ttgatgggga ttggtgggct
    18001 tatccacagt ttagttgatt ataatattgc ttattttata gcaggaacac tcagcaaata
    18061 ttttgatatc cccgtattta cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg
    18121 gtacgcgaag aaaacgacat attagacatg atctctcaaa aaaaaaactc tcatctagta
    18181 atggataata aatcctctaa gatgagagtt ttttgtccta aaaagagctg caccaacatc
    18241 ttctggcaca gctctaacag ggggcaattt atggaagttt gatgacttgt tctgtttctt
    18301 tatcaaagaa gtggcctttg ctgatgttga aggcaaggtc gactatttcg cctggtttat
    18361 ggaaatctct tgcatctact tttgagatga actcagtaga accagttttt gtgtaaagca
    18421 tcgtttcagc tccaagtaat tcggaaacga caacttctga tttcactacg gcttcaggtg
    18481 aagcttcaag ggctacttgt tcactgtgga tgtcttctgg acgaataccg aagataactt
    18541 cttttccttc ataacctttt tcaactaata ctttgttttt tccttcagga atacgaagct
    18601 taagcccatg tccatctgtg ataacaccat ttttcaatgt cacattaaag aagttcatgg
    18661 caggagatcc aataaagccg gcaacgaaca tattgacggg tgtatcataa acttctttcg
    18721 gtgtaccgat ttgttggata aatccgtctt tcatgataac gattcggtca gccatagtca
    18781 ttgcttctgt ctgatcgtga gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt
    18841 tggcaatctc agcacgcatg gctacacgta atttagcatc taaatttgat aatggctcat
    18901 ccattaagaa gacttttgca tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt
    18961 gtccaccaga taaagcagct ggttttcgtt tcaagtattc tgtcagacct aagatctctg
    19021 ctgcattgtc cacacgtttt ttgatgtctg ctttatcata tttacgtaat ttcaagccaa
    19081 aagccatatt atcaaaaaca gtcatatgag gatacaatgc gtagttttgg aaaaccatgg
    19141 caatatcacg gtctttaggt gccacgtcat tcatgacttt tccgccgata tttagttctc
    19201 cttcagagat atcttccagt cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg
    19261 aaggaccaac aaaaacgata aattcacggt ctttgattga tagattaaaa tcagttacag
    19321 aataattttc tgcattgtca tattttttat atacgttttt cagagccatt tctaccataa
    19381 tacgttccac actttctttt ttagttttat taactattta aagtataaat gaaagcgtta
    19441 actaacttca atgtcatgtt gaacaagaat tttttctttt ttttgtcaat gtgctagttt
    19501 taagctttaa taaatatcca taagaaataa aaactgatac actggagtaa ataagtatgt
    19561 taacaatatc aaaaggagtg tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa
    19621 aacctatgat ggtgaaatta gcaacagcgt ataaaacaat attggaaaaa catgaacttt
    19681 ttgctaccgg gacgactggg cagaggatca cggaagcaac cggcttgccg atccatcggt
    19741 ttaaatccgg accgttaggc ggggatcagc aaattggagc catgatttcg gaggatcagt
    19801 tagatttggt gatttttctt cgagacccgc tggctgcaca gccccatgaa ccagatgtaa
    19861 cagcacttat ccgtttatct gatgtctatg agattccgtt agctacgaat atcggaacag
    19921 ctgaggtgct actaagagga ctggaagccg gcttcctaga ttttcgaaga atcgtccatg
    19981 aaatagacaa gcatccactt tcattttgat ggataagcgt atggaaaaat aacgtttaat
    20041 gttcgcctat atgtagctat aaaagctaaa caaactgata atatgcgata taatacgaac
    20101 gtattggata atataaacaa taatattagt ttttcgtatt tgcagtaaat gtgaaaatat
    20161 gctatagttt ctgtgggaaa agatcccaaa aataatacag agtagaaaat aaagcgttaa
    20221 gtgctaagag gatgggatgt tgcctcttgg acgaaggacg taatgttcgc ggttttattt
    20281 tcgcattcgc tgcatgatac gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa
    20341 caccggttag acgcacgtat gattgctatt atgggcttat tgattgcatt gatggtcact
    20401 ttgtcccgac tggtagcaat cgagacccca tttattaaaa tcagtgtcac atttattcca
    20461 caagtgatca tgggcattct ttttggtcct ttttggtcag ggataggcgc agttttagct
    20521 gatttagtag gaatggccct cttctcgaaa tctgcattct ttattggatt tacattgaat
    20581 gcatttattg aaggagcaat ctacggattc tttttctatc gaaaagagat cacttggaaa
    20641 aatgccattc tggctacgct tagtgtcacg ctgatcatca acttattttt gacaccatta
    20701 tggcttgcgt tgatgtacca tgtgccattg ttcagctggg tcgtttgggc gccccgcctg
    20761 ttaaaaacag taatatggtt accgattcaa tcaattgcca tttattacgt aggacgtagt
    20821 attccttaca aaaaaatttt aagaagttta gcgatccacg ctaaataaaa aacaagcagt
    20881 cttcccatcc cagaagactg cttgttttta ttgtgctgtg aattgtgctt gatagagccg
    20941 tcggtaataa ccatcttttt gaagcagctc atcatgggtg cccatttcga caatagctcc
    21001 ctgatccatc actaatatct ggtccgcatt tttgattgtg gacaggcgat gggcaatcac
    21061 aaaactggtt tttccttcca tcattgctaa gaaggcttgt tggatttttt tctctgtcaa
    21121 tgtatcgact gagctggtag cttcatctaa gatcaacata gggggattac tgatcatcgt
    21181 tcgagcaatc gtcaacagtt ggcgttgtcc atctgatatc tttaaccctt gaccgccaat
    21241 cttagtatct aatttttccg gcaaacgttc tacaaattca tacatataag aggcttttag
    21301 ggcatcattg atttctgcgt cacttgcttc cggattccca aaacgaagat tttctcgcaa
    21361 tgtactgtca aatagccaag tatcttgtaa caccattcca aaacttttac gtaatgtatc
    21421 acgttggata gagcggatgt catggtcatc gactgtaatt gttccttttg ttgtgtcata
    21481 aaaacgcatc agcaaattaa caagtgtgga ttttccggcc cctgtttttc ctactatagc
    21541 aatggtttga cctggataag cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc
    21601 ataagaaaaa gaaacatgat caaaagagac ttttcctttg atatttttgg gttccaaggc
    21661 ataggcatct ggcaaatcag gcttttcttc tggctgatcg atcaaatcaa atgctcgttc
    21721 tagaccagaa aaagcggttt gcagttgtgt cgtgattcca gaaagttcga taaatggttt
    21781 agaaaactga cttgagtaga tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc
    21841 actcttcaat gccaataggc cgccaactaa accaatcgat aaataagcaa ggtgatctac
    21901 aaaacgagaa gacgggtttg tcaaggaaga agaaaattgt gcccgttgtc ctttttcata
    21961 tagatcagca tttagcagtt caaattgctg ttgactagct tcttcttgct gaaaagcttt
    22021 cacgattttc tggttaccga ttcgttcggt cacaaagccc gagatatccc caataatcct
    22081 ttgctgtcta tcgaaatttg tttgtgaagc acgagcaacg atccaactca caaggaaaat
    22141 aattggtgta gaacaaagaa cgaccaatgt cagaagtgga cttagacgca tcataaaaat
    22201 aaacgaaaga atgatgattg ccactccaga aaataattga ttgaaggccg cagaaattgc
    22261 aatggaaata ttgtccatat catttgtaaa tcgactgacg atatttccgt gggagttttg
    22321 atcataataa cgaagtggca atcgattcag atgagcaaat gcatcttttc ttaattttgc
    22381 aacggataag taagctaacc gattgcttaa cacttggatg agccattgac tgactacgtt
    22441 gattaaaacg ataccaccga atagaataag taaatggaac agtcgattga agcggacctg
    22501 tccttctcca atcatttgat cgatactttg accaatttgt aacgtcatca caactgttgc
    22561 aatcccattg atgattccta gtatcacagc tagccaaatg tcttttgcat aggcagtaag
    22621 gtaaggaatg aatcggcgga gcgtctgcaa ggataatttt tttactttca taactattct
    22681 tcctcctgtg atgcgacgat ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg
    22741 gtaccctgag cagcaatctt accttgatcc atcactaaga tatgatgtgc ttcttgaata
    22801 gagcggacac gttgggagat aataatcact gtactgccaa gataagtctg tagcgcctta
    22861 cgcaaatcca agtctgtttg ataatccaac gcacttaaag agtcatctaa aatcagtaat
    22921 gctggcttgg caatcaaagc tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa
    22981 tttttcccat tttccatgac ttttgtatct aaaccatttg gaagctgacg gacaaattct
    23041 tctgcctgag cgattttcag agcttcccaa cattcttttt ctgtggcgtc tttcttaccc
    23101 cattgaaggt tttcacgaat agtcccggta aatagtacag aagtttgggg aacaagagca
    23161 atttgctgac ggagatgatc cagctgccaa gaacgaacat ccattccgtc cattataact
    23221 gtaccggaac tgacatcata gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt
    23281 ccggttggac caatgattcc aaggactgta tttcgcttca gctgaaatgt aatattttgg
    23341 agagaaagac cagaatcagg agtataacgg aagtcaacat ggtcaaaagc gataattgct
    23401 tcagaagaaa aatcgggagt agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct
    23461 agcacctctt tcacccgatt tcctgaagct tcggcacgtg tgaagatcac gacgaggttt
    23521 gaaacaacga tcagtgccaa aagcatttga ttcatataat tgatcaaagc aagaacttgt
    23581 ccttgttgca agctaccaat atttactttg atcccgccaa ctgtcagcaa acagatgatc
    23641 ccgacattca tgatcaaggt tgtagcaggt gataataggg cagagatatt ggaaacacgt
    23701 aaataattgt tcgctagatc atttgtgctt ttatcgaact tctctgtttc tttttttgtc
    23761 tgcgcaaatg cgcggatgac acgaacaccg ctaagattct gactaacgtg ttcattgagc
    23821 tgatctagtt tttcttgcac tttttggtag agaggaacgg tttttttgat gataaaaaat
    23881 aaaatgattg agaaaatagg caataatgct aagaaaaaca gacctacttc ccagtcgatc
    23941 acaaaagcca tgaccactga cccgatactt aaaaacggtg ctctgatcaa taaacgaatg
    24001 accattgcta aagctagctg aagctgattg atgtcgtttg tcattcgggt gatcaaggta
    24061 tctgttccaa aatgattcaa ctcgttgtga gagaaagtat tgattttttt gattagctga
    24121 ttacgtaact ctgtaccaaa tccttgtgaa gctactgaag catagtattg gcaaatgata
    24181 gcacaaatca gaccaatcac agacatagcg accatccata agcccatttc aatgattttt
    24241 cctcggtcat tcatttttag tccattatcg atcaaggaag ccatcattaa aggcaggaca
    24301 agttcgaaga cagcttcaag aaatttgaag aacgggccca aaatgatttg cttacgatag
    24361 tcttttgcat aacgtaataa ctgaatcata tgattcctcc tattatttat ttgtccacaa
    24421 ttgaaaaaac aagtactacc acattgtagc ttaggaaatt tagaatgaaa agagaacaag
    24481 ccttttcata aatgaaagtt ttattatgaa gaagctttag aaattgaata gactattttt
    24541 tttaaaaaaa agaaaatgag aaaacatgcc agtctcttca ttcataggaa cgtctatttt
    24601 acttaatttt tctataaatg
//
LOCUS       DAFGZE010000040        24391 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.041, whole genome shotgun sequence.
ACCESSION   DAFGZE010000040 DAFGZE010000000
VERSION     DAFGZE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.041"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(187..2532)
                     /locus_tag="LTU54_001735"
     CDS             complement(187..2532)
                     /locus_tag="LTU54_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBL8296450.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKNEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            2811..3713
                     /locus_tag="LTU54_001736"
     CDS             2811..3713
                     /locus_tag="LTU54_001736"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBL8296451.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            3924..5699
                     /locus_tag="LTU54_001737"
     CDS             3924..5699
                     /locus_tag="LTU54_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBL8296452.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(5765..7351)
                     /locus_tag="LTU54_001738"
     CDS             complement(5765..7351)
                     /locus_tag="LTU54_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBL8296453.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            7602..7826
                     /locus_tag="LTU54_001739"
     CDS             7602..7826
                     /locus_tag="LTU54_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HBL8296454.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            7968..9719
                     /locus_tag="LTU54_001740"
     CDS             7968..9719
                     /locus_tag="LTU54_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8296455.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLERELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            9719..11503
                     /locus_tag="LTU54_001741"
     CDS             9719..11503
                     /locus_tag="LTU54_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296456.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQEFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            11838..12690
                     /locus_tag="LTU54_001742"
                     /pseudo
     CDS             11838..12690
                     /locus_tag="LTU54_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            12787..14673
                     /locus_tag="LTU54_001743"
     CDS             12787..14673
                     /locus_tag="LTU54_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8296457.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAADGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMSLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVINYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(14714..15616)
                     /locus_tag="LTU54_001744"
     CDS             complement(14714..15616)
                     /locus_tag="LTU54_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL8296458.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            15846..17249
                     /locus_tag="LTU54_001745"
     CDS             15846..17249
                     /locus_tag="LTU54_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL8296459.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            17246..18682
                     /locus_tag="LTU54_001746"
     CDS             17246..18682
                     /locus_tag="LTU54_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBL8296460.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            18827..19753
                     /gene="trxB"
                     /locus_tag="LTU54_001747"
     CDS             18827..19753
                     /gene="trxB"
                     /locus_tag="LTU54_001747"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBL8296461.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            19966..21693
                     /locus_tag="LTU54_001748"
     CDS             19966..21693
                     /locus_tag="LTU54_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBL8296462.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            21709..22059
                     /locus_tag="LTU54_001749"
     CDS             21709..22059
                     /locus_tag="LTU54_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBL8296463.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(22153..23013)
                     /locus_tag="LTU54_001750"
     CDS             complement(22153..23013)
                     /locus_tag="LTU54_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBL8296464.1"
                     /translation="MSSDSTEIITFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            23105..23533
                     /locus_tag="LTU54_001751"
     CDS             23105..23533
                     /locus_tag="LTU54_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL8296465.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            23695..23871
                     /locus_tag="LTU54_001752"
     CDS             23695..23871
                     /locus_tag="LTU54_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBL8296466.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            23898..24344
                     /locus_tag="LTU54_001753"
     CDS             23898..24344
                     /locus_tag="LTU54_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBL8296467.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT         7721 a         4361 c         5191 g         7118 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctctggtttt
       61 tcacattttt acataaattt gtcttaatat cgtagatgta agcttttgaa caccatttat
      121 tcggaaaaag ggagctgtga caagattttt gtcgcagctc cctttaattc tgattaatct
      181 attagatcaa tcatctttat cgtctgattc tttggtctct ggattcgctc tagcataagc
      241 tgaagcggtg ttccagtaat ctgtatagtt ttcatcagta ccgcctacgc cgaatttaaa
      301 ggcgcttttc ttcggaccat tttttgccca aagtgactcc actgtttttc ctggcgtttg
      361 gatggatttt ttattgacag taacttttcc agctgtttgt cctgtgaacc cagatacttt
      421 tttcttcatc acatcggatg ataagtcaaa tttctcatca gtgccgaaaa tctcagggtt
      481 tgcttggtaa gcttgattga ttagattggc aagataatta gccgaacgta ttcttgcttg
      541 gctgtccatt ccagtattgt catcgtgtcc tgcccaacta ctgatagtga tagatggagt
      601 tgagacgatc agccatgaat ctcggtattc attggtagaa ccagtttttc cgacccaatc
      661 tgcatttcct agatttccgt ttaagcttga gatgacatct ttgaatggtg tagtgatttt
      721 ttcattgatc acactacgca tcatgtcatt catgatcgat gccgttgctt cggaataaat
      781 acggacagaa ttactttcat gtttataaat cacattacct gcattatctg tgatggaatc
      841 aatgatatag ccttcttcgt agactccacc atttgctaag gcctggaatc catttgtctg
      901 ttgaagcgtc gttacatcta ctgtccctaa aggagccgat tcaacgcccc aattatcatt
      961 tgctgggtag ttcatcttcg ccaagtagtg ctcgtaagcg tattgtttcg atccaccgga
     1021 attcaacaca tcttgatata agtgataagc tgggatattg ttggaccatt ccagtgactc
     1081 acggacggtt tggaaggtgt tcgacccttc attggtcgca ttgacgattt tctcaccagc
     1141 attttttcct tcttgccaag tcgttgcata atctgataca cgggattctg atccgacaag
     1201 tcccatatcg attgcaggac cgtaaacaag aaccggtttg atggatgaac cagcttgacg
     1261 ttctgtatca aaggcgtggt tattttgatt ctggctgtaa ttccgaccac ccacgaagcc
     1321 atagatacgt ccggttctgt tatccatcaa tacatttcca gtctccactt ggctaccagc
     1381 accatcatct aacatgtagc cataattttc tacgccggtt tgtaatgcag catagatatc
     1441 tttatcgatc gtcgaatgga tcgtatatcc tttgttttgg atcgtttgct gtgctttttg
     1501 ataataagct tcactgaccg atgaatctga catatcagca ttatcttttt ctgccaattg
     1561 ttgtgcaata atccttgttg cttcgttcat cacggtataa tacaagaatt ctctgtcagt
     1621 ctgttccgcg atttgttgtg ggagaaagtc ttttgtaaga tcataggctt tcgcttcttc
     1681 gtattgttct ttcgtaattt gtccttcacg gtacatactg aataaaacaa aatcttttct
     1741 ggcaagaccg gcagaaagat cttcttttag cgcgcctgta ttcgtgtatg gactatacgt
     1801 gatagggctt tgaggcaaac cagcgatata agcagcttgt ggcagagtca catcctttgc
     1861 atctacacca aagattcctc tagcagcttc ctggactcca gcaatatttt gccccttatt
     1921 attccgacca aatggtgaga cattcaaata cgtagtaatg atctcatttt ttgaaaagtg
     1981 tttttctact tgtgcagaaa gaacgatctc gtttgcttta cgtttgaatg ttgtttcatc
     2041 ggttaggatc tgttgtttga ctaattgctg tgtcaaagtg gatcctccag acgagccgat
     2101 gccagtcgct tctgaaacta gtgctcgtaa aacagcttta gggacataac cttgatgttt
     2161 atcgaagtat tcatcttctg tactgataat cgcggttttc aataaaggcg aaatcttatc
     2221 agatgaaata gtcgtccgca tcagatctgt ctggattttt gatatctctg tattatctgc
     2281 ataaacaatc tttgacgttt ccgtaatgtt tcctagttct gtctgcagtt cttttttagt
     2341 cggcaattgt gtgtcttcga caagataggc aaagtagcca gcacctattc cgatacctag
     2401 agctgcgaac aacaaaagaa gaacagtaat aaaaacagtc aatgattgaa acactctaaa
     2461 aataatctta ggaacaaacc atttttcttt ttttcttcta ttctgttttt ttcgagtatt
     2521 tttgcgtggc aaaagggtca cctcaactaa tggttttatt tcgctaatta tagcaaataa
     2581 taggacaaaa aaacagacaa ggctgttttt gacaggaatt tctttttcga atgagataaa
     2641 acgtgaggaa atgatctttt ttatgaaaga gttcttaagc ttttctttag ggaaagtttc
     2701 agttactatg gagaacaggg caaagaaaaa caaaccattt tctttgtaca tcatcttatc
     2761 aatatacagg aattgttcga aacaattgcc tttcaaggag gaatccatcc atgttattag
     2821 gctcacatgt cagcatgagc ggaaagaaaa tgctgttagg cgcggcagaa gaagcagcca
     2881 gttacggatc ttcgaccttt atgatctata caggcgctcc tcaaaatact agaagaaaag
     2941 ctatcgaaga gatgaatatt gatgccggaa aagcatttat ggcagaaaac ggactaacaa
     3001 atatcgtcgt tcatgcgcct tatatcatta atttgggaaa tacgatcaaa ccagaaatgt
     3061 ttccatttgc tgtacaattt ctgagagaag agattgtccg tgcggaagct ttaggtgcta
     3121 ctcaaatcac ccttcaccca ggcgctcatg taggagcagg ggcagaagct gggattgcta
     3181 gaattattga ggggttgaat gaagttttga caaaagaaca gacggcacag atcgctttgg
     3241 agacgatggc agggaaaggt acggaaatcg gtcgaacatt tgaggaatta gcgcaaatca
     3301 tcgatggagt aaaattgaat gaaaaattat ccgttacttt tgatacatgt catacgaatg
     3361 atgccggata taatataaaa gaagattttt caggtgtaat ggaagagttt gataaggtca
     3421 tcggattaga tcggcttaaa gtactgcata tcaatgattc taaaaatcca caaggttctc
     3481 ataaagatcg gcatgccaat atcggttttg gaacaattgg atttgacaca ttgaacaaaa
     3541 tcgtccatga tccaatgttt gacaacattt cgaaaatatt agaaacccca tatgtcggta
     3601 aagataaaaa gaaagcttac gcaccatatg gaaaagaaat ttccatgttc aaagcgcaag
     3661 cattcgatcc cgaagtgttc cctgagttat tagcagaaga agtcacactt taagttttct
     3721 acctgacagc aacaaaaaag gattgggcag tctgccttgt ccttttttgt ttaagaaaca
     3781 aaggagtgct ttttgcattc gttttcgcca tgctcctttt gccccctctt tttaaattca
     3841 gacaaacagg gtatactgat agaaaagtaa aaaacgaaaa gtttcacaaa caagtagcaa
     3901 aacgtgattg aaaggacgaa aagatgctga acattttaaa ccccttgatc gaccaactaa
     3961 aaaagaaaca tacatacttt attcttggca tagccgtatt atttacaatt attcggttgt
     4021 atttatatag taaagcaatc tatggaacag atatgtcggc gacctatgat gatcagctgc
     4081 tgattcacta tgctcaaaat attttggatg gtaaatggct aggggagtat acgactacaa
     4141 cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa ctgcatcttt
     4201 cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt gcactcgcgt
     4261 ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat tttttgtttt
     4321 ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc ctcgtgattc
     4381 cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa tacggttcta
     4441 gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc ttttggttca
     4501 ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt atcagtttcg
     4561 tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta aaaaaacgac
     4621 tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt cctatagttg
     4681 gcttttccat cgtctatcag tggatcgaat ataaaaatga gacaacatac ggtattgcaa
     4741 ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg atccgaatag
     4801 atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat gctgcactaa
     4861 ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt gacgaattat
     4921 atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga gatatcatct
     4981 tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat gcaaaagaaa
     5041 ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac aagggaacca
     5101 tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg atgagtgatc
     5161 tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga tataaagatt
     5221 tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag gctcaagaat
     5281 tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga acacctaatt
     5341 tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac caagttctgg
     5401 ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta ggaaccgtat
     5461 ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc ggattaatac
     5521 tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg ccagaaaaaa
     5581 aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa ttcatcgtag
     5641 taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct tctaaataaa
     5701 aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt ggaaaaatca
     5761 gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt ttgtttcctt
     5821 tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg attacataag
     5881 cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag aaactttcga
     5941 tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag aaccagccaa
     6001 gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg ctgttggtca
     6061 tcatcagtaa aggaatcatc gaaaatggca acgcaaaagc caagaacacc tgggattcat
     6121 tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt gtaaacaaaa
     6181 cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt agccaaatcg
     6241 ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta ccagtaatag
     6301 tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag ataccagatt
     6361 ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa gcttcataca
     6421 gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca cccatgatca
     6481 atagcagtgc attgactaga aaagccattg acaactggat attagaatcc caagtagaaa
     6541 aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga gtttgtgaaa
     6601 ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc cctaaagctc
     6661 cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga ctgttaggga
     6721 taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga tagacaaaaa
     6781 cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga aacccgactt
     6841 ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca atcaccaatg
     6901 gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgcg atgtctgttg
     6961 ccataatggc taattcagtc aagatccata aaatgacacc caatgtctta cttgtccgtg
     7021 ctcggattgc ttgggccaaa tccatttggg tcacgattcc taattttgct gccatatact
     7081 gtaaaagcat agcaattaga ctagacatca aaataactga catcaataaa tattggaaat
     7141 tttgtccacc agtgatcgaa gtggaccagt tacctggatc catataacca actgctacta
     7201 atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa actttttccc tttggtacat
     7261 ccactgttcc atttacttca gctaaagata aaccatttgt atgttcaata agtcgatgct
     7321 tatgaaattg tttgttggat tgattattca agaaaaaacc acctttccta tttatctacg
     7381 attacattct acacctcaga aagaaaaaat taaagaaaaa gattcggtat gcctaatttt
     7441 ttttcgtggt gtgtcttgct ggttaatgaa agaaattttg ttcttctcta aaaaaaccac
     7501 tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt tagtaaaata
     7561 aatcattgag agcaattcgg aaatagaaag gagagattta tatggtatca acaggtattg
     7621 tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt ttcctagcgc
     7681 gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac atgttgcgat
     7741 caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag atgatgcagc
     7801 aaatgaagaa ccaagggaaa aaataggttc tgacccaaag actttttgcg ggctgatttt
     7861 ttcagtccgt tttttatttc gtaatttgtt agactattca gactattcag caaaaaagac
     7921 aagcacagac aatcctatgc tcttgataaa gacaaaggag gagtaaaatg tcgattttta
     7981 gaaaattagg ttggtttttt cgtcaggaaa agaaacatta tttaattggg attttctccc
     8041 tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc atcattgatg
     8101 agatcgccga taagaattta tcaataaaga tcatttcctg gtggatctta ttattgatcc
     8161 ttgcagcttt cttacaatat ctttttcgtt atatctggcg gacgaatatt tggggaagtg
     8221 cagctcggtt ggagagagaa cttcggcagc agctattcaa acattttaca gaaatggaca
     8281 atctctttta ccaaaaatat cggactggag atttgatggc tcatgcaacg aatgatttga
     8341 atgcaatcca gaatgtagca ggagctggaa tcttgacctt tgcagattca gtgattacgg
     8401 gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca ctgattgcgc
     8461 tgattcctct gcctttgcta gccgttactt cacgagtgct ggggtcaaaa ctccacgatg
     8521 cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa gaaagtatca
     8581 ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctcgcg gattttgaag
     8641 aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac tccttatttg
     8701 atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt ttaggtggaa
     8761 agtatgtctt ggaaggcacg atctcaatcg ggcagatggt ctcatttatt agctatatcg
     8821 gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg gaaagaggaa
     8881 atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc gtggaacaac
     8941 tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc tcttttaact
     9001 atccgaaaga agaatcacca gcattagaac aaatcaaatt tgcgatccaa gaaggagaaa
     9061 cattaggtat tgttgggaaa actggtgcgg gaaaaacgac aatcatcaaa ttactgatgc
     9121 gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc aaagaatatt
     9181 cattgaatgc tttattagga tcaatcggtt atgtgcctca ggatcacttc ctgttttcga
     9241 tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa gaagtagagc
     9301 atgcggcttc tttagcattt atcaacaatg aaatcaagga attgccaaaa ggttatgata
     9361 ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacagagg atttcgattg
     9421 ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga tgatgcactt tctgcagtag
     9481 atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa gagaaaacaa
     9541 ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc aaaagaaatc attgttgtcg
     9601 atcaagggaa aatcattgaa cgaggaaccc accacgagct gcttgcatta ggtggctggt
     9661 atgaacgaat gtgggaaaaa cagcaattag aagcgaagat agaaggaggc gaggcctgat
     9721 ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac agaagaagat
     9781 catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg ttgctatctt
     9841 atttgcgttt gccctgtctg tgatcaatgt cctattgcca aggatcatcc aaacttttat
     9901 ggatgatcat ctggctaaac agtcagctac gacacaagtt attttgtttt ttgctggtgt
     9961 gtacttgttt ggagtcatag tcaaaagtat catttggttt ttccaatggt atttgtattc
    10021 catggcttca ttgaagacct atcagtatgt tcgggtaaga ttatttgaaa agctgcatac
    10081 actaggtatg cgttattttg accagacacc ggcaggatcg accgtttctc gagtgacgaa
    10141 tgacacagaa acgctatttg aattttggta tgtctttttg atggtattga ctgggatctt
    10201 tgcagtcgtt tcttctttcg ttgccatgtt tcaaatcaat ggagaaattg ccttgtggac
    10261 tttggtgttc ttgccgattt taggaatcgt catctggtat taccagaaat tcagttctcg
    10321 tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga atgaatcgat
    10381 ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta aagaatttga
    10441 acaagtgaac aacgagtatt tagacacgcg ctatgcgatg atcaaaacga attcgattct
    10501 gttaagtcca atcatcaatt ttctctatgc attggcgatt gcgttggcgt tgactttgtt
    10561 tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat ttacgaccta
    10621 tgtccaggaa ttttttaatc caatgtctca aatgatggac tttttgagca cctttacaga
    10681 tgggatggtt gccggtagtc gtattttgaa aatcatggat acagaagaat atgcaccgca
    10741 acaaaatcct ggagcagatc aagcgattac ttcaggtaag atcgagtttc gcaatgtaag
    10801 cttttcttat gatggagagc acaatgtatt gaacaatatc tcttttgtcg ctaatccggg
    10861 tgagaccgtt gcattagtgg ggcacaccgg aagcggaaaa agctcgatca tcaacgtatt
    10921 gatgcggttt tacgaatttt atgaaggcca aatcttgatt gatgatcatg acattcgaga
    10981 atatccaatg gaagagttac gtaagaaaat gggattggtc ttgcaagatg ccttcttgtt
    11041 ttatggagat attgcaggaa atatccggat gatgaatcca gctatcacag acgaacagat
    11101 tcgccatgca gctgagtttg ttcaagcaaa caagttcatc gatgaattac caaaaggata
    11161 tcactcaaaa gtgatcgaac acggtgccag ctattctagt ggacaacgac agctgatcac
    11221 ttttgcgcga acaatcgtga cagatcctaa aatactagta ttggatgaag caacagcaaa
    11281 tatcgatact gaaaccgaag gattgatcca agaaggattg gcaaaaatgc gacaaggacg
    11341 gacaacgata gcgattgccc atcggctgtc cacgattcgg gatgcagaat tgattttagt
    11401 tttagacaaa ggtcatatag tagagcgtgg gaatcatgaa acacttctag cgcaaaatgg
    11461 tttgtatgca gatatgtaca aactgcaaag cagtggtgtg taaaaagagg ttgtgacagt
    11521 agcatagtag cattttgccc taagcgctag agtgaaaata aaacagagct tcgcagattc
    11581 ttcatctgtg agcgttagtg gcgtagtcac ccgatttaaa aaagacgacg acagctagtc
    11641 aaaagcggct aataaccact aagaaagcgt tgacaaaagc gtttgctttc gataatataa
    11701 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    11761 atggttccat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatatatc
    11821 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    11881 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    11941 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    12001 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    12061 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    12121 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    12181 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    12241 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    12301 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccaacaaga caatacctac
    12361 ctgttcacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    12421 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    12481 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    12541 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    12601 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    12661 cgtttagtgc atgagaatcg ttgtaaataa ataaaaaaag gaggataaat taacaagaac
    12721 gtatgaaata agggtagtga cattcagttg gctaaacctt ccactaataa attggaggga
    12781 atagaaatgg gaaaatacca agaattagcg agaaaaatcg tagaaaatgt cggcgggaag
    12841 gaaaatatca atggtttgac acattgtatc acaaggcttc gtttcaaatt aaaaaacgaa
    12901 gaaaaagcaa atgatgagat cttgaaaaat atggacggta ttgtaacggt aatgcgagcg
    12961 ggagggcagt atcaagttgt catcgggaat catgtgccag tcgtgtttga agaggttata
    13021 aaagcaggaa accttacttt tgatgaggct gtttcaacag aaaaaatgag accgtttgat
    13081 atgcttattg atggaatatc gggctgtttc caaccattcc ttgctatcct agctgctgac
    13141 gggatgattc gtggactgac agcattcctt gtcttcttag gcgcgtttga ccgcggttct
    13201 ggcacatttg ttatgtttga taatatcggt gattctgtat tccaatttat gcctgttatt
    13261 atcggtttaa cagctgcccg taaatttaaa gtaaatgaat tcgtagggat gttgcttggc
    13321 gcagcattaa tgaatcctag cttgtcgctg gaagctttga gtggtgcagc agaagcacca
    13381 ctaacgacca tattttctgg cactattttt gaagcaccta tttatcaaac agtattcggc
    13441 attccctgga ttgcgcgtaa ctacgcttct tctgtcattc ccattatctt tattgtgttg
    13501 ttagctagcc aaattcagaa accaattaaa aagttagtac ctgagatgat cgcgaatttc
    13561 ttcgttccat tcttcaccgt tcttattaca atgcctttag gttttttact cgttggacca
    13621 gtattcacat tcgcaactga tattttaatg gctggatttg aaacactttt agcactttcg
    13681 ccagttattt atggagcaat cgtaggcttc ttctggcaaa ttctggtgat gtttggtctc
    13741 cattgggcaa tcgtaccaat gggattgatg caattttctg tgaatggttg gcaaaatatt
    13801 atgacacctg ttgcagtagt gtcttttgga caaactgcag cacttaccgc actctacttt
    13861 aagcttagaa atccaaaaga caaagcaatt gcaataccag ccattgtttc tggtattgtg
    13921 gggattacag agccagctat ttatgggttt acattgccac gtaaaaaaat atttattttt
    13981 acctgtgtcg gtggtgcgtt agggggcgcg tatagtgggt taatgaattt aacttcttgg
    14041 aatcaaggtg gactaggcat ctttacaatt cccaactata ttagaccaga tggtgattta
    14101 acagatgtga ttaatgtatt gattgggatc gccattgcga tggtggtatc ctttgcgctg
    14161 acttttttct tttggaagga ggaagcagat gaaacagatg atagacagaa aagatcaggt
    14221 aaagaaattg taaaaacacc gatacaagga caaatcgctc cactgaatgc agcaaaggat
    14281 gcagcttttg cacaaggaac actaggtcga ggaatcttga tctaccctga aaaaggagaa
    14341 gtacgtgccc cttttgacgg aacagtcatg tccctgtttc cgacgaaaca tgcaatcggg
    14401 atggtttctg aagcaggtct ggaattgctg attcatgtcg gattggatac tgttcagttg
    14461 gaagggaaat attttgaaag cttggtgcag caaggagaaa gagtgaaaca aggcgatctg
    14521 ttgcttcgct tcgacaaaaa gaaaatcgaa gaggaaggat acagtttaga aacacctgtg
    14581 attgtcatca attacatgga ttatttggat attttggaaa atcaagggga aacggtcggg
    14641 ccagacgatg cacttatcac cgcactgacc tgaacggtta ggtgaaagaa tggactaggc
    14701 ttaactattc tttttagtcc acttctttat tttcaaaatt gaaaggctct ttcatgatgg
    14761 ctgagctgat agggcgttta tcaaattttt gtcctacaga aattagatgg actaaattct
    14821 gttgataatt ttctgcaaaa acacaactat ataacacgtc tagaagataa caaatcgatg
    14881 tattgatcgt aaaattacca attttcgaat acagtcgttc gcgtgtcgta agtcgcaaag
    14941 cggctgtact cattgaagat agcgttgaat caccgatact ggtcaaagct aaaattggca
    15001 catgattttg tttcaatatt tctgcggtac gaaggatcac attgttttca cccgtataag
    15061 aaatcagaat cccacacata tcacttgtgc aattgtacgc ttcatagaac gattcaccaa
    15121 aagtggtgac aatcgtgaca tccattttga tgcgcttcat tttcagcatg aaatcttgag
    15181 aaatcaaagt attcgagtta ctggcgaaaa tcacgatttt ttttgcttga agcattagtt
    15241 gtttggcttt ttgcagttgg tcatgatgaa tgagagaaag tgtatcttga atcgtcatct
    15301 gttctaaaga agccatttta tttgcgatcg tcataatacc gtctgtggtg gaaaaaggaa
    15361 gattcgcatc tacgtcttga aaattccctt caagataggc tgtttcagct aaaaaagcat
    15421 tacgaaaact gacccagcca tcaaaaccca ttttttttgc tacacggatg agtgtggacg
    15481 ggtggacata acatcgctca gcaatctctt ggatggtcat ttcgccaagt gctggagaat
    15541 cgcttaagag aaactgaatc acttcatttt ctgaaggaga aaaggagatt tctttgattt
    15601 tttcacgtaa taacatgtag ataccttctt cttgtttttt tacgtaaatc ataacttttt
    15661 tgaaaatgat ttgtaaacgg atttatgtta tatagcttaa taaagacatt ttttgacaag
    15721 atatttacaa aaatccagaa gaaaacacta acttttgtaa atgttactgg aaagaaacga
    15781 atttctattg tttgttttgt ttacaaaaaa gataataaaa gtgtaaaaag taaaggagtg
    15841 agaacatggc aaaaggaatc aaaatcgcta cgattggtgg aggcagcagt tacacccccg
    15901 agttgatgga aggatttatc aaaagatatg aggaattacc gatacgagaa atctggctcg
    15961 tagatgtaga agaaggaaaa gaaaagcttg agatcgtcag tcagatgtct cagcgtatgt
    16021 gggatgcttc tccgtatgaa gtcaaaatct atgcaactct ggatcgggaa gaagcattga
    16081 aagatgcgga ttttgttacg actcagtttc gtgtcggtca attagatgca cgaatcaaag
    16141 acgagcggat ccctagttat tatgggatga ttgggcaaga aacaaatggt gctggcggga
    16201 tattcaaagc ttttcgtacc gttccagtgg ttttggcaat cgttgaagac atgaagcgtt
    16261 tatgtccaaa tgcttggctg attaatttcg cgaacccaag cgggatggtg acagaagcag
    16321 ttatcagata tggcaaatgg gataaagtga tcggattatg caatgtgcca gtaatggcga
    16381 tgatgacaga accaacaatg cttggcaaga aaaaagaaga gctgatctac aagtttgccg
    16441 gtctaaatca cttccattgg cacaaagtag cagataataa aggaaatgac cgcacggcag
    16501 aattgattga tcgtttatac gatgaagaca atggattgcc gaaaaatatc tttgacatcc
    16561 cgtttttcaa agaacaatta aaacaaatga acatgatccc ttgtggctac caccgctatt
    16621 actatcgtga agaagaaatg ctggaacatg ctttagaaga atatcaaacg attggtacaa
    16681 gagcgcagca agtgaaagaa acagaagcag agctgtttga attatacaaa gatccagaat
    16741 tggatcataa acctgaacaa ttgcaaaaac gaggtggtgc ttattattca gatgctgcct
    16801 gtgaaacgat tgcttcgatc tatggaaata aagaaacaca gatcgtggta tctacaaaga
    16861 atgatggagc tgttcctgat ctgccggcag attgtgtagt agaagtcact gcctatgttg
    16921 gcgcacaagg cgcacgaaat gtagcgtttg gttcccttcc tgctgctgaa agaggctggt
    16981 tgcaagtaat gaagaatatg gaattgctga cgatcgaagc agccgtgact ggtgattatg
    17041 gaacagcttt gcaagcgttt acgatcaatc cgcttatacg atcaggaaag acagcaaaac
    17101 ggatcatgga tgagttgttt attgcacatc agcagtattt accgcaattc aaggaaacaa
    17161 tcagccggtt aaaagcagaa ggaatcgaag tccaagatga actggcaaga gaattatcaa
    17221 gcaatacaca tgtaatggag gaagtatgat gggattcaga aaagattttt tatggggcgg
    17281 agctacagca gccaatcaat gcgagggcgg ttatgatgaa ggcggacgtg ggttagcgaa
    17341 cgtagatgtt gtgccaatcg gagaagaccg ttttcctgtc attactggaa aaaagaaaat
    17401 gtttgaattt gatgaaaatc atttttatcc agcaaaaatt gcgatcgata tgtatcatca
    17461 ttataaggaa gatattgctt tatttgcaga aatggggttc aagacttatc ggatgtccat
    17521 tgcttggagt cgtatttttc caatgggcga tgaaaaagag ccaaatgaat ctgggttgaa
    17581 attttatgag gatgtcttta aagaatgtca aaagtacggg atcgagccgc ttgtgacgat
    17641 tacacatttt gattgtccga tgcatttgat caaagaatat ggtgcgtggc gttccagaga
    17701 actcgttggt ttttatgaaa atctgtgccg agtcatcttc aatcgctata aaggacttgt
    17761 caaatattgg ttgacattca atgagatcaa tatgatcctt catgcaccat tcatgggcgc
    17821 agggctgtat tttgaagaag gagaaaatga agaacaaatc aaatatcaag cggcacatca
    17881 tgagttggtt gcaagtgcca tcgcaacgaa gatcgctcat gaagtagatc cggagaacaa
    17941 agtaggctgt atgctggcag caggagcata ttatccatac acttgcaatc ctgaagatgt
    18001 ttgggcttct agaaagtcag atcgagaaaa ttatttcttc attgatgtcc agtcacgagg
    18061 agaatatccc gcttatgcat tgaaagaaat ggaacgaaat ggcattcaga tcaaaatgga
    18121 acctggggat gccgcattgc tgaaagaaca tactgttgat tttatctcct tttcttatta
    18181 ttcttctcga gtttcgacta ctgacaacga actcttggaa cagactgcag gaaatatctt
    18241 tgcttctgtc aaaaatccat atctggaagc aagtgaatgg ggttggcaaa ttgatccatt
    18301 aggcttgaga ataacgatga atgatattta tgatcgttat caaaaaccac tatttattgt
    18361 agaaaacggc ttaggtgcag tcgatacgcc cgatgagaat ggttatgtgg aagatgatta
    18421 tcgaatcgac tatctggcag cacatatcca ggcaatgaaa gatgcagtag aattagatgg
    18481 agtcgatcta ttaggctata cgacttgggg atgtattgat ttagtatcag ctggtacagg
    18541 tgagatgaag aaaaggtacg gattcattta tgtcgatcgt gacaacgaag gaaatggcac
    18601 attagcccgt tcgaagaaga aatcattcaa ctggtataaa aaagtaattg attcaaatgg
    18661 agaaaatctt tcgaacgatt gatcaatgat ttataaaagt ctattgtttt tcagcggatt
    18721 tttcgagtat aataaagcta ttcgagaaac gcaagcagaa caatagactt tttttcgcat
    18781 tggctgtaaa gtatgcttac ttgattaagt aaaggagaat ctacatatgt atgatgtaat
    18841 cgttattggc gcagggcctg ctgggatgac tgctgcttta tatgcttcac gttcaaactt
    18901 atccgtattg atgatcgaac aaggcgctcc tggcggccaa atgaacaata cggcagaaat
    18961 cgaaaactat cctggtttcg attcaatcat gggtcctgat ctagcttata agatgtatga
    19021 aggtgtttca agatttggga cagaaaatgc ttatggaatc gtgcaagata tcaaggatca
    19081 cggcagccac aaagaagtta tttgtgaaga taaaacttac gaaggaaaaa cagtcattat
    19141 cgcaacaggc tgtatccacc gtaaattagg cgtacctggc gaggaagaat ttgctggacg
    19201 tggcgtttct tactgtgcag tttgtgatgg tgcatttttc cgtaacaaac gtctagtagt
    19261 gatcggagga ggagactcag cagtagaaga agccatctat ttgacacaat ttgcttctga
    19321 agtagtcatc gtccaccgta gagatgaatt acgtgcacaa aagatcatcc aagaccgtgc
    19381 atttgcgaat gaaaaaattt cctttatgtg ggatacagtc gttgacgaaa tcgttggaaa
    19441 tgaaatggta gtgacaggtg tgaaagcacg taatgtcaaa actggcaatg agtttgattt
    19501 ggcagcagac ggagtattca tctatgtagg attggatcca ttaactgcac catttagaga
    19561 agctgggttg acgaacgaag ccggctggat tccaacagac aacgaaatga agacgaatat
    19621 accaggcgtt tttgccatcg gagatgttcg tgaaaaagat ttgcgacaaa tcactactgc
    19681 tgttggtgat ggtgggattg ctggacaaca agtgtataaa tacttggaat ctttagaaga
    19741 aacaactgca taagtttaaa aagagtgaaa gaacggtttc aattgttctt ttactctttt
    19801 ttttgcgtga aatctactga atccgattta tgcgaatact tgtaaccgtg cttgataaaa
    19861 gaatcttttc ttgatttaag cgttttttgg tcctcatact ttcatatcta ataagtgaat
    19921 gctatactta ttaagaacca tatttcagaa agaggtgtta ttttcatgtc ttgggaacaa
    19981 gtgtatgaac aatgggtaca tgaagaaaat ttagaagaaa acttaaaaaa acaattaaat
    20041 gatctgggtg aagatcctga aaaacttgaa gatgcttttt atgcaccgtt ggaattcggt
    20101 actgcaggca tgcggggaat cttaggacct gggatcaatc gaatgaacat ctatacggta
    20161 agacaagcaa cagaaggact tgcacgtttc atggatacac aaggtgaaga aacaaaacgc
    20221 cgaggtgtcg ctatcgctta tgactcacgc catcaatcac cagaatttgc catggaagca
    20281 gccaaaacat tagcacatca caatatccct tcttatgtat tcgaaagttt acgtccaaca
    20341 ccagagcttt cttttgcagt ccgtcatttg aagacgtttg cgggaatcat ggtaactgca
    20401 tcccacaatc cagcagctta caacggatat aaagtctacg gagaagatgg gggacaaatg
    20461 cctcctgcag acgcagatgc attgacgaaa tatgtccgtg aagtcagcaa tccgttaaaa
    20521 gttgaagtat tatctgacga cgaagctgaa catagcggat tgatcacgat catcggagaa
    20581 gaagtggatg ccgcttatct agaagaaatc aaagcagtaa cgattgatca tgaattggtg
    20641 gaaacaatgg gtaaagattt gaagcttgtg tacacaccac ttcacggaac aggaaaaatg
    20701 ttgggcgaac gtgcgttgaa acaagctgga ttcgaacaat tcgtactagt tccagaacaa
    20761 gctgtcgcag acccggattt cactacagtc aaatcaccga atccagaaga acactctgct
    20821 ttcgagtatg cgatgcgact tggcgagaaa gaaggcgctg acttactgat tgcaacggat
    20881 cctgatgcag accgtttagg cgcagccgtt cgtttgccaa atggctccta tgaagtattg
    20941 acaggaaacc aaatcggagc cctgtttgct caatatattt tggaagcaca ccaatcgcga
    21001 ggcactttac ctgaaaatgc agcggtattg aaatcaatcg tttctagcga attgcctaca
    21061 gcaattgcgg aacactatgg ggctaccatg ttcaatgtat tgacaggatt caaattcatt
    21121 gctgaaaaaa ttcaacaatt tgaagaagat cattcccata cttttatgtt cggattcgaa
    21181 gaaagttacg gttatttagt taaacctttc gttcgcgaca aagacgcgat tcaagcattg
    21241 ctcttattag cagaagtagc agcttactat aaaaaacaag gcaaaacatt gtatgacggc
    21301 ttgcaaacga tttttgaaac atatggctac tacgcagaaa aaacgatttc tgtgacaatg
    21361 agcggacaag aaggctctgc aaaaatctct gctttgatgg aaaaattccg tgaagaagca
    21421 ccgacagaat ttgccggagc aaaagtcatc caaacagaag acttcaaaga gttgacgaga
    21481 acagaccgtg aaggaaacgt agaaaaacta acgacaccgc catctgacgt attgaagtat
    21541 acgctggaag aaaatggctg gatcgctatc cgtccttcag gaactgagcc gaaaatcaaa
    21601 ttttatatcg gtgtaaaagg aaactcttca gaagacgcag ctgaaaaagt agccgcttat
    21661 gaagcgatta tcaaaaaaat gacagaagaa taaaaaaact tgagactgat ggaaaaagtg
    21721 gtaaacttta gaagtcagtc ttttttcaag ttggaggtga atggattgcc agacacgcaa
    21781 aaaacaaaag tagagaaagt cagccaacta ttcaaaatgt tgagtgaccc cactcgtctc
    21841 aaaattctgc tgtatctgaa agacggggaa caaaatgtga cagcaatcac ccaagcagta
    21901 gaaatggaac aatctgctgt atcgcatcaa ctgcgtttat taagagaaaa ccatgtcgtc
    21961 aagtcccatc gtgagggaaa agcgattttg tatagtcttg atgattctca cgtactggat
    22021 attttgaacc agacgctcaa acatgtggaa catcaataac gataggtgca aatagagaca
    22081 aactggactg tatcaacgaa tcttcttcgc tgacacagtc cagttttttt aagaaaaaga
    22141 caagaaaaac tgttagtctg tttcttcggt ggtttcagtt ccataataac tatgatcttc
    22201 taggtctaaa gcattcaaaa taagtgaagc tgtttcttcg atagataaag aagcgacatt
    22261 gattactacg cagcccaatt tttcataaag atcattcgca aaatcgagtt cagctcgtat
    22321 tttttcgata tcggaataag cagtatcagg atttaatccg taagagcgca tccgttcttt
    22381 acggatattg tttaaaatat ctgggttatt cgtcagtccc acgatttttt taggatcgat
    22441 ctcccaaagc tgtttgggaa tatgtgcttg tgggatcaaa ggtagattgg caacctttaa
    22501 atttttgtta gctaaaaaca agctcaaagg cgttttggaa gttctggata cgcctagcaa
    22561 aacgacatct gcttctagaa acccacgagg atctttcccg tcatcgtatt ttacagcaaa
    22621 ttccattgct ttgatccgtt tgaaatagtt ctcattcaat aaatgaaggg cgccaggttg
    22681 ccgaatgggc ttgataccag ttaagcgctc gatttctgat atcggcggag tcatcacatc
    22741 aaaactaaat aattgatgtt cttcacaaaa agaacgcgtc atctcgatca attcttgatt
    22801 gatcaatgtg tgtaggacaa gggctttttc ttttagtgca tcttgcagtg cctgcatcaa
    22861 tgttttctca tctgtcacaa atgttctgcg gaataaattg aactcaacgg taggatattg
    22921 ggccatagtg gcttgcgcta attttgtcgc agtttctcca gcagagtcag agatgacaaa
    22981 aaaagtaatg atttctgtag aatcggaaga catggataaa acctcacttg catttttttt
    23041 atgtttatta taccatactc tagaatgtgt acttcgtaat cattttgatt tggaggaata
    23101 agtcatgaaa aaacagacat tgattgaaca atcactagaa acattgaaaa ataacggttt
    23161 taaatatact aaaaaaagag aagcattgtt aacttattta gtgaagcgca atcgctacgt
    23221 atccgctaaa gaagtttttg actatatgaa tcaggaattc aaaggagtaa gttacgatac
    23281 gatttatcga aatctccatg attttgcaga aatcgattta ctggaagaaa ccgatttaaa
    23341 tggagagatg aagtttcgtt ttcattgctg tcaaggggaa atcggccatc accatcactt
    23401 tatctgtacg gtgtgcggaa caacgaaaga aatccatatg tgtccaatga attatttcga
    23461 agaacagctc ggtggatgta cgatcgaagg gcatagattc gagattttcg gaagatgtga
    23521 aaattgtcaa taaggattta aataaaataa catttgataa ttttaaaaaa aagcaagatt
    23581 tccttgacgg tcaaaacagc attagatata atatttacag gacagtcttg ttttttgaga
    23641 tatatttcaa taacaattac ggttactttt taagctcgga gggagggaat taacatgtca
    23701 aaaacagtcg ttcgcaaaaa cgaatcactt gacgatgctc ttcgtcgctt caaacgttcc
    23761 gtgtcaaaag cgggtacttt acaagaatct cgtaaacgtg aattctacga aaaaccaagt
    23821 gtaagacgca agaaaaaatc tgaagcagct agaaaacgca aaaaattcta gttctgtgat
    23881 gaattggagt tggtagtgtg tcactactaa gtagattaaa cgatgacatg aaaacagcga
    23941 tgaaagcgaa agataaggaa tctttacaag tcatccgaat gatcaagtca tctatacaaa
    24001 acgagcagat taaagaaggt cacgacttga cagaagaaga agaattaacg gtgttgtctc
    24061 gcgaaatgaa acagcgacgt gattctttgc atgagtttga agaagcagga cgtgacgatt
    24121 tagctgaaaa agtaaaaagt gaaattgtga tagtcgaaaa atacatgccg gaacaacttt
    24181 ccgacgaaga aattcgacaa cttgttcaag aagcaatcac acaaactggt gcttcatcaa
    24241 tgaaagaatt tggcaaagtc atgggtgcga tcatgcctaa agtcaaagga aaagcagatg
    24301 gaaaccaagt aaatgccatt gtgaaagaac tattacaatc ttaattttat catttaaagg
    24361 ctcttcgtca atgagtgttg gtgaagaaat t
//
LOCUS       DAFGZE010000041        23048 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.042, whole genome shotgun sequence.
ACCESSION   DAFGZE010000041 DAFGZE010000000
VERSION     DAFGZE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.042"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(172..627)
                     /locus_tag="LTU54_001754"
     CDS             complement(172..627)
                     /locus_tag="LTU54_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296468.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(781..1989)
                     /locus_tag="LTU54_001755"
     CDS             complement(781..1989)
                     /locus_tag="LTU54_001755"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBL8296469.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2101..2433)
                     /locus_tag="LTU54_001756"
     CDS             complement(2101..2433)
                     /locus_tag="LTU54_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HBL8296470.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2581..3456
                     /locus_tag="LTU54_001757"
     CDS             2581..3456
                     /locus_tag="LTU54_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL8296471.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3538..4119)
                     /locus_tag="LTU54_001758"
     CDS             complement(3538..4119)
                     /locus_tag="LTU54_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HBL8296472.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4125..5384)
                     /locus_tag="LTU54_001759"
     CDS             complement(4125..5384)
                     /locus_tag="LTU54_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBL8296473.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5386..6432)
                     /locus_tag="LTU54_001760"
     CDS             complement(5386..6432)
                     /locus_tag="LTU54_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296474.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6667..6942)
                     /locus_tag="LTU54_001761"
     CDS             complement(6667..6942)
                     /locus_tag="LTU54_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBL8296475.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7185..8495)
                     /gene="der"
                     /locus_tag="LTU54_001762"
     CDS             complement(7185..8495)
                     /gene="der"
                     /locus_tag="LTU54_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBL8296476.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8677..9906)
                     /gene="rpsA"
                     /locus_tag="LTU54_001763"
     CDS             complement(8677..9906)
                     /gene="rpsA"
                     /locus_tag="LTU54_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBL8296477.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(10041..10721)
                     /locus_tag="LTU54_001764"
     CDS             complement(10041..10721)
                     /locus_tag="LTU54_001764"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBL8296478.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10770..11405)
                     /locus_tag="LTU54_001765"
     CDS             complement(10770..11405)
                     /locus_tag="LTU54_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL8296479.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11471..12874)
                     /locus_tag="LTU54_001766"
     CDS             complement(11471..12874)
                     /locus_tag="LTU54_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBL8296480.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12861..13892)
                     /locus_tag="LTU54_001767"
     CDS             complement(12861..13892)
                     /locus_tag="LTU54_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296481.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13923..14144
                     /locus_tag="LTU54_001768"
     CDS             13923..14144
                     /locus_tag="LTU54_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBL8296482.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14309..15046)
                     /locus_tag="LTU54_001769"
     CDS             complement(14309..15046)
                     /locus_tag="LTU54_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HBL8296483.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15199..16539)
                     /locus_tag="LTU54_001770"
     CDS             complement(15199..16539)
                     /locus_tag="LTU54_001770"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBL8296484.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16776..18299)
                     /locus_tag="LTU54_001771"
     CDS             complement(16776..18299)
                     /locus_tag="LTU54_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBL8296485.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18677..19288)
                     /locus_tag="LTU54_001772"
     CDS             complement(18677..19288)
                     /locus_tag="LTU54_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL8296486.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19411..19536)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19618..20334)
                     /locus_tag="LTU54_001773"
     CDS             complement(19618..20334)
                     /locus_tag="LTU54_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBL8296487.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20340..20909)
                     /gene="scpB"
                     /locus_tag="LTU54_001774"
     CDS             complement(20340..20909)
                     /gene="scpB"
                     /locus_tag="LTU54_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBL8296488.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20893..21690)
                     /locus_tag="LTU54_001775"
     CDS             complement(20893..21690)
                     /locus_tag="LTU54_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBL8296489.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21660..22031)
                     /locus_tag="LTU54_001776"
     CDS             complement(21660..22031)
                     /locus_tag="LTU54_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL8296490.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(22050..22937)
                     /gene="xerD"
                     /locus_tag="LTU54_001777"
     CDS             complement(22050..22937)
                     /gene="xerD"
                     /locus_tag="LTU54_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBL8296491.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6463 a         4928 c         3910 g         7747 t
ORIGIN      
        1 ggttcattct gacccagatt tgattttgta caacccatca gtccttattg acaaagagcc
       61 caaaaatctt gtcacagtcc cttattctga ataaacggtg tggaagagac agcctatcag
      121 tagtaataag acacatctct ttcacagcct catttttgcg taaaaatcga tttattcgaa
      181 aaattccccg tatacatctt ctacacgttg atctggattg atcacgatat acaagttacc
      241 tgttttttcg ctgatttttg ttgattgata ttgattgtct aatccttctg ttcctttatc
      301 tagatatggg aagtacacaa gatcaggtgt gccatttcgg taaaggattt ccattcgctc
      361 gatatcttcg tatttcaatg cgcgattaaa catgcccatt tccagtccgc ctaggttgat
      421 atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg attttaaatc cagcacacgg
      481 atggatttca acaaattcac tgccattgat tcgtccaaaa cttgttgtga tttgtttgat
      541 ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt tctccatttc taaaataaaa
      601 ttttagcgct gtcatttctt tactcattaa aaagactcct ttatgatgta atttattctt
      661 gtactacctc agtttagcac aaatttatgt aagtggtcac atcttatgtt ttttcttcta
      721 atgacgtatc tgcccacttt aagtagccaa caaagctgag cagatacagt ctagtgtttt
      781 ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt tttttatcat tttttattct
      841 ttgttctacg acggcttttt cagctaattg caaagcttca ctaacccact taccaggttt
      901 ttttccagtt tcttctagca agattttacc atctactgct aattcgctta atgacttgat
      961 aggtaaacga tcataggatt ctcctacggc atccatctga ggtgtttcgt caaaatagat
     1021 catcaattct tctacttgca gtgcatgttt tttgcctaag aagtacaatt cgaatggttc
     1081 caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt gcttgaacgt ctttaatcat
     1141 ttggttagaa cacttccacg atttcaaaaa ggaacgaatc tcattttctt ttagatccag
     1201 ttggctgatc aataatgccc atgcttggct ctcttctttc aatgggacgg atggtaattc
     1261 agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg ggacagcaaa tgtagcactc
     1321 tgtttcaata aatgcatgga ttcccgcggc ccgttcattt cccaagagca tcttgatgaa
     1381 ttccacattt atacgttcta cagatatttt acctaatagc tcgtggtttt ctttgatggc
     1441 gtagaaggtt tcttcctcta atttgaaccc tagttgactg acaaaacgca acccgcgcat
     1501 cattcgtaag gcatcttcat ggaaccgctc atgaggattg cctacagctc gcaagatctg
     1561 ttcttctaga tcttgcagac catcgaacaa atcgatgatt tctccatctt ctttcaaagc
     1621 aaacgcattg atcgtaaaat ctcttctttt caaatcttct gccaacgaac gaacgaattc
     1681 gacatgatct ggtctacggt agtcctgata agttgattcc gtccgaaatg tggtgatctc
     1741 atattgttca tcattttcta ataccaacac agttccatgt tcgatcccaa tatctatcgt
     1801 ttttggaaaa agctgtttga tttcttctgg aaatgcgctt gttgcgatat ccacatcatg
     1861 gattggctgg ttcagcaaga tatcacgaac acttccacct acaaaataag cttcatatcc
     1921 tgcattctct aattttttca gaactggaat tgccttttgg tattccagag gtaattgttt
     1981 taatttcatt tttccaacct ttcctgcgtg tttcttgtcc tgagataaag actcagttgc
     2041 atggtgactg tttgacaagt ccgtttcaga gtcttcaagg ccaggaccac ttcatcttgt
     2101 ttactcgtcc ttacgctcaa agcgattatg gtctctttga ttgaattttt ccatgttttt
     2161 ttcaaaaact tcagttaagt cgatattcaa tgaattagcc atgatcatcg taacgaataa
     2221 aacatcacca agttcttctg ctaccgagtt aactggttca gaagcctttt tgcttttttc
     2281 tccataagtg tggttgactt ctcttgccaa ttcacctact tcttcagtta atcgagccat
     2341 ctgagctaat ggagagaaat accccacctt gaattgctgg atatattcat ccacttcctg
     2401 ctgcatactg tataaagaac gctttttatc catctttttc cctccaaaac tatcttacca
     2461 aaactttaac gcaaatccta gataagagca attgagaaaa tcgaatttcc atgctacatt
     2521 taggaaggaa tttctaaatg gaaattctgt gaaaatacat aattgaaagc tggaattcat
     2581 atggtcaaac gaaaaacagt aaaagaagtt ctttttatca ttataggaac aagtatttat
     2641 gcatttggtc ttgtttactt gaacatcgct aatcatttag cagaaggggg agtctcaggg
     2701 atcaccttga tcttaagagc tttatttggt attgatccag cttatacgac tttattaatc
     2761 aacatcccat tgattttact aggaggaaag atcctcggta aacgttcctt ggcctatact
     2821 gttctaggta cactttcttt gtcattcttt ttatggatct ggcaaagggt tccactagaa
     2881 atcaatttac agcatgatct actgatcgtt tctctacttg cgggtttagt tgcaggtgta
     2941 ggaagcggga tcgtctaccg catgggtgga acaacaggcg gcagtgatat tatcgctcgg
     3001 attttagaac aaaattacgg catcactatg gggcgttccc tattggcatt tgatatcatc
     3061 gtcttacttg cttcattaac ctatatcgac ttgaaacgta tgatgtatac gttgattgtt
     3121 tcgtatgttt tcagtcgtgt gattgattcg atcttagatg gcggatattc tgcaaaaggt
     3181 ctacttgtcg tctccaacaa aagcgaagag attgcaccac tactgatgac cggtctgcaa
     3241 agaggtgtga ctttcctttc tggagaaggc gcattctctg gtaattcaaa aaaaatcatc
     3301 tatatggtcg ttagctctag tgaattaact gaagctaaga ggatcgtcca cgagattgac
     3361 gaaaaagctt tcctttcaat catcaatgtg catgaagtgg aagggcaagg ctttacttat
     3421 ttgaagccac agacaagatt actgaaaaaa ctataagaaa aaagacaaag ataggctatg
     3481 aggaaagatt ccacatccag ctgacctttg tcttttttag tactatacta tttttaacta
     3541 attgttatgt tcacttaccc ggttactttt cttttgtgtc tgcaaaagga tctttcggtt
     3601 aacttcatca gataatttca aaaaagcatc cctatctcca tcttctagtg cttgatcgat
     3661 ttgagcataa agcattttta gttttgcctc ttcttctttt tcttgaaaat atcgttcaat
     3721 taattcagta tcttcttcac tgactgaaga atcccaagaa gaaaatggat tgtcttctaa
     3781 gatagataga tattctcttg tctgccaagc attttcgaat atacattcca catataaagg
     3841 ctttttccaa ttcaaacgga tttcatgaaa aatctggtct gtgtccgtaa attcttttcc
     3901 ctgcaaaaac aatttcatgg gttccccttc aacagataga tcactgatct gcagtcctcg
     3961 atccgcaaaa ttcgcccctt caacaaagtg gacattgctt aaaatcgctt catgattggc
     4021 aagataattc aagatccaaa aaacttctct tcggctaaaa gatacatgat tgaccatcca
     4081 cgttaaaaaa ttcttctttt cccgtacatc aattagcatt tttctcacct ctctgttagg
     4141 tcatctaata atgacagtgc ctccatatct cctggttcaa gtgttaaata ataagtcaat
     4201 aattcttgcg ctcttttcag ctgcccttct tctcttaaga aaaaggcata ctctttcaag
     4261 aaatctggtt catgtgatag ttcatgataa gcctgttcat agtgtacagc tgcaacggta
     4321 aagtcttcta attcattata agcatgggct aggttccatt ctgcataagg attagcagtt
     4381 tcttccatct gattgatcac ttgtatcaca tcttcatatc tttcatcatc caagtaaaga
     4441 ttacttaaag taagtaatgt ttcatcttgt ttgtctccta cttctaaagc tttcagtaaa
     4501 agttcttctg ctcgctgtct atcatgcaga cggaaagcat tttccgaggc caggtgataa
     4561 agttcgactt ggtaaggatt ttctttgatt ccttcttcta acactgtttg tgcttcttca
     4621 atcatttctt cttcttgaag agcttgtcct agatatagat ataacgattg ataatgcgga
     4681 ttaacttctc gaagttcttc cagatagttg attgcctgct gattttcttt caattgaaga
     4741 ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt cttctttcag tgcttcttct
     4801 aaaggtacga tagctgcttc gaattctcct tgcatactta aggattgtcc taaacgttcc
     4861 tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca acaatgtttc ataaaccact
     4921 gctgcctctt tgaaacgatc aactgaaaaa tacaattcag ctagcgcaaa ctggatcaat
     4981 ggttcatcag gaagcaaccc agctgcttct ttcaattttg cttcactcac ttcagggatt
     5041 ccgataactt ggtacaaatc agctatcgcc aataagcttt gcggatagta atcactttgc
     5101 ttatccactt tttccaggta ttcaaaagct aagtcgatct cattatcttc gattgcaatt
     5161 tctgctaacg ggatgttcag tccgtcattt tctgggtatt ggttgatcag ttgttcaaaa
     5221 atctgttttg cttcgatcaa aaagccaata gccagcaatt cttcgcctag ttcagctaat
     5281 atatctgctg ggtcttcttt taatgctttt tctaaaaaga tttgtgcttg tgacaagtct
     5341 tcatctgata aagcttgcaa cattttttcg ctatttgtat tcatttcaat tccctcttat
     5401 ctcactgacg atttgttgaa ataccgtcat tgttctcaat aattcttttt cgtttggatg
     5461 ttggtatccg tctattatac ctattttttc taaaacaagt atttttttgc ttttttctgc
     5521 caaaaaaaga actttttcca aatactccat ctttgaaatc tgttccagcc agtggattgg
     5581 atagccaagc aagtccagcc acacaaaaaa gttttttagt tggaaatcaa aaggatatgc
     5641 ttggagattc cacacgaaat gaaacatcat tcctaaaaat tttttcatac tagcagaaag
     5701 tagatggcca ttttcggtca gataaaaagc ttgttcaaac agtttaccat aatttgacag
     5761 gttttctgcc tctaattgat aaaaatagat catttcttcg atcatcccgg caaacggccg
     5821 agtcgttaat tgctttttat ttggatagtt tttatacaga ttttgcaaaa aacgatgatc
     5881 acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc tgctttccgt ccacctgccg
     5941 ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga ttgatggttt gcagcactac
     6001 acgatttggt agctgcaaaa tcgtccgctg cggtgttaat gctcggctta gagaacggat
     6061 cgatacaggg atcatccata gtgatgaatc aagggttgta tgtttttgaa aaaaacctgc
     6121 cagatccatg atcccttcat ttccaaatcc gatgataaac atggattgat tttcgggata
     6181 gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa tgattgcatt gagtgttggc
     6241 acaaatatac cagtcattgt ttgcttgatt tgcaaataac gggttgatct tatctgcaaa
     6301 caaatcataa tagcgctgat tcgttaaaaa taagattggc tgatcaatgg aaagttctct
     6361 tagctgactg actagcgttt ctccgtaaaa cactttcgtc aacgcatctt ctacttgata
     6421 atcaagttcc atacgcatcc ctcctcttct tatttttatt ttaccattag taagcaaatt
     6481 ctagaaaaga taagagcaaa gctcacctca taacttggaa ataaataaaa attaaataaa
     6541 atacatgtgt tttctatgta tgacatacgg attcggaatc tctgtctctt atttattcct
     6601 tttctgctag gaaataaaaa gaaaagcact tgtagcccta gagccacaag cgctttttca
     6661 actgtcttat ttaacagcgt cttttaatgc tttacctggt ttgaatgcag gtactttgct
     6721 agctggaatt tcgatttctt caccagtttg tgggttacga cctttgcgag ctgcgcgttc
     6781 gcgaacttca aagttaccaa aaccgattaa ttgaactttt tcgccgttag ctaaagcatc
     6841 ttgaatagtt gaaaatacag catctactgc tgcagttgca tcttttttag ttaaatcagt
     6901 agctgaagca actttttcga ttaattctgc tttgtttgcc atggatagat tcacctcctc
     6961 cacaagagat atgatagaaa tatcatttag ttaaaacttt ctgagtggtc cagaagttta
     7021 aaaagaaata ttgattaact tcaatattct gttatcacga tatcatatga accgcttaat
     7081 agcaaggttt ttaccaattt tcacatgctt tttttagtca tagaaacgta acaaacgaaa
     7141 ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa atgcctattt tctcctacga
     7201 gggataattc ggatggacgt cccttcaaaa gtaaatgctt ttctgatttg attttctaag
     7261 aaacgttcat aagaaaaatg catcaattcc tcttcattga caaaaacgac aaatgtaggt
     7321 ggtttgacag cgacttgtgt agcataaaaa atcttcaggc gttttccttt atctgttggt
     7381 gttggattga ttgctacagc atccataatg atatcattca acacagccga agagatacga
     7441 agattctggt tcatacttac ttgttcgatc atttcaggca gacgttccaa ccgttgttta
     7501 gtcactgcag aaacaaaaac gattggagcg taatcaagat accggaattc atcacgaatc
     7561 tcttgttcga aatcacgcat tgtattggtt tctttcttta caagatccca tttattcacg
     7621 acgataataa ttccacgacc agcttcatgg gcgtaacccg ctactcgctt gtcttgttcc
     7681 cgaatccctt cttcagcatt cagtaccatc aaaacgatat cagaacggtc aatggcacgc
     7741 attgcacgca tcacgctgta tttttcagta ttttcgtata cttttccgcg tttacgcata
     7801 ccagctgtat ctatcattaa gaatttctga ccactttcag aaacgaagtg ggtgtcgatt
     7861 gcgtctcggg tcgttccttc aatatccgaa acaattacac gttcttctcc taaaatcgca
     7921 ttgatcaaag aggatttacc aacatttggg cgaccaatca aactaaactt aatcgtgtcg
     7981 tcgtcctctt cttcagtctc ttcagagaaa tacttcactg cttcatctaa aatatcacca
     8041 atccctagtc catggcttcc tgaaacagga taaggatcgc ctagccctaa ggaataaaat
     8101 tcatagatat cgtttctcat ttctggatta tctactttat tgactgctaa aatcactggt
     8161 ttattacttc tgtataaaat tttcgcaacc atctcatctg cgtctgtcac gccttcgcgt
     8221 ccgctgacta cacaaatgat tacatctgct tcttcaatcg cgatttctgc ttgatgcttg
     8281 atctgatcca taaaaggttc atcgccaaga tcaattccgc ctgtgtcgat cacgctgaat
     8341 tctcgaccta accattcacc ttttgcataa atacgatcac gagttactcc aggagtatct
     8401 tcgacaatcg agatacgctc accagcgatg cggttaaaaa tcgtagactt accgacgttc
     8461 gggcggccaa cgattgcaat tgttggattt gccatattat ttcctcctca caagtttcca
     8521 atctcactta gtttaacgaa tgcattgcca actttcaagc atagattgtc aattaaacga
     8581 agtattttta ttccttattg aaatactaat cgcttttcaa aagaaaaact gagacaaaag
     8641 cagctgcttt catctcagtc acagatacaa aatcaattat ttttcagaat catcggttgc
     8701 tttttcttct acttcttctt tcaacgcatc acctaaaatg tctcccatag taaatccagt
     8761 attttcttct ggtaattcat aagactcaac attttttggt tcttcttctg aagctggttt
     8821 ttcttctaaa gctttgatac tcaatgcaat acgatgttct tctggatgga tctctaatac
     8881 tttcactttc acttcgtcac cttcatgaag cacttcatgt ggtgtcgcaa tatgtttatg
     8941 tgaaatttga gagatatgga ctagcccttc tactccaggg aacacttcaa caaaggcacc
     9001 gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg ccaactggag cttttgtatc
     9061 gatttctgac caaggtccag gctgtgtgtc tttgattgac aaggaaacac gttcttgttc
     9121 aggattgata gacaacactt tgacatttac atcatcattg acagccaata catcacttgg
     9181 ttttgctaca tgactatgtg aaatctctga gacatggact agaccgtcaa ttccgcctag
     9241 gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt ccactgagaa catctccctc
     9301 atggatagtt gaaagaattt cttttttctt ttcttctctt tcgttttcca ctaccgcttt
     9361 atgcgataag atcaaacgat tttctgatgg ttcgatttcg atgattttaa agctcaatgt
     9421 tttgccttta tagtctgaaa agtctgcaac aaaatgatct tccaccattg atgcagggac
     9481 aaaaccacgt acaccgacgt caacgactaa accgcctttg acaacatttg taacaggtgc
     9541 ttcgatgacc ttgccctctt ggaaatctct ttcgatatct tcccaaactt ttttagcatc
     9601 taaacggcgt tttgaaagta ggtagctgcc gttttcttta tctttgccga tcgtactgat
     9661 aacgactaaa tctaatacat caccgacttt tactacttcg ctgatatctt ctaccggcaa
     9721 agttgataat tcttttgccg gaacgactcc ttcaactcca gccccttcga ttccgacaat
     9781 cacttgtttg tcttcaatga ctaaaacttc gcccttaaca acatcgccaa cttttacttc
     9841 ttgtacgctt ttcattgcgt cttccattga ctggttgttc tcttgatttt gatcaaattc
     9901 tgtcataaca cttgtcctcc taaccaacat tctgccgtaa gtacggtact gtttttagtt
     9961 taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa aaacaattgt
    10021 ttttcatttt ttttcttaat ttaaaaaaga agataacctt ttttcaaaac aacagctttt
    10081 attgctgcaa cgacttctgg aatcgatttg ccagtcgtat cgatacgaac agcatcctct
    10141 gcttgcttca gcggagagac gtcccttgtt gaatcataat ggtctctttc ctgtatctct
    10201 ttcgtcagtg tttcaagatc agtcggaatt cctttttctt ggttctcttt gaaacgtctt
    10261 tctgcacgct cacttacgct tgctactaaa aagattttca cttctgcatt cggtaataca
    10321 gctgtcccga tgtctcttcc gtccatgaca acgccaccgc gttcccctat ttttcgttgg
    10381 acagcaacta attcttctct gacttttcca tgtgcagaga cttcagaaac agcttttgtg
    10441 acatctggtt gacggatttc taaagtcaca tcttttgatg ctatgaatac atgctgtcca
    10501 tcttcttgtt gcgaaaaaga gataggatac tttttgatca gcgaaactag cccttcttca
    10561 tctgaaaaag atacattgtt cgtaatagct aaataagtta ctgcgcgata catggcaccg
    10621 gtatctgtat aaatataatt caattctttt gctaatatct tggcaacggt acttttccca
    10681 gatgaagcag gcccgtcgat tgcaatactg atttgtgtca tgatataact cctttttctc
    10741 agaaaaaaag ccgcaaagcg cggctattat tatttaattc ttaattcttg accagggtaa
    10801 agcataaagt tgtttggatc taagccattc aattcaaaca attgatcagt ggtgatccct
    10861 gcacgctcag ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt tgaaccggct
    10921 gcagctgatg aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc tgcttgataa
    10981 gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc tgacgttaca
    11041 cctgtatctt gagacacagt agattcttcc gcgattgtac tagattctac tgttgaagtc
    11101 tcagttgact cctcttttgt tgtacttgta gaactttcta ctactgatgt cgaagtttca
    11161 gaagaagttg ctgctgttgc attgttgctt gatccatttc tgatccagaa ataagcacct
    11221 gctggaatag cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa taatgtattt
    11281 cctttctttt gttgtctctg agaacttctt gatgaaactt catcatctgt atcgtagatt
    11341 ggttgttccc atggttcttg tgtttcgtta ttttctgtag attgatgtct atcttttctg
    11401 ctcactgttt tacctcctaa actctatcgt gtgtattata ccataggaaa aagtgtcttg
    11461 tcctaaaaaa tcaatgttcc atgttttttt taaataaatt tagtaaaatc gtttcccaat
    11521 gaagttgttc agtcttagaa aaaagcttcc tctttggcag aagttctgct tttgctccat
    11581 caatatcaca acaattctgc ggcttttcgg agagtttttc gccaaaataa gctaagatga
    11641 attttctgcg gcagttttct tcattaatat agcgaagcat ctgctgcagg cggaattctt
    11701 tttctttttc ctgacgtttg agccgttcca cccaattttc tggtttttca gactgctgga
    11761 tcagctccat ccattttttt tgcagctcat caaagggagc ttgctttgtg gcatattcca
    11821 aataaagctc aaaactttgt cgctgttctc tagaaagttg attaaagaaa taatggatac
    11881 gttcatcccc actttgataa aaaagaattg ctgcactttc ttcctgatct ctgcctgctc
    11941 gaccgatttc ttgtacatag ttttctagac tgtctggcaa atcataatgg accacataac
    12001 ggatatcaga tttatcgatc cccataccaa atgcattcgt agcaatgaga aactgcagct
    12061 gattttttac aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga ccaccgtgat
    12121 agtatccaac tgtaaaacgt tccctgaata aatgatacaa tcgttcgact tctttttttg
    12181 ttgcacaata aatgattgcc gctccttgtg ctctttccat gaagtgttca agttcttgtt
    12241 ctttttgctg cgttttccga acaaaaagcg agatattttg ccgattgact gattgtctga
    12301 tctcttttgg tggtcgatca aatagtacat gacgtatatc tcttgctact aaatctgttg
    12361 ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt tttcctaact
    12421 gttgatattc tggacggaaa tcaactcccc attgagacac gcaatgagcc tcatctacta
    12481 catataacgc aatattttgt tttttcagat gctctaaaag agaaggatta gtcaacattt
    12541 cgggactcaa aaacaaaaat ttatattgcg atagatgacg gaggacgtat cttctttcag
    12601 attgcgaaag taaactattg aaagcagcta ctcgtttctc ccctcgtttt tgtaattgcg
    12661 tcacttgatc ttccattaga gaaagcagcg gtgacacgat gatgaccaga ccttccatta
    12721 aataccccgt caattgatag caaaggcttt ttcccgttcc tgtaggcaaa atagcgagag
    12781 tatcttctcc atcaagcaac gcctgaataa tttcttcttg gccgggacga aatgaatcaa
    12841 atccaaaata tttttttaat tcatttatta acattctgta tcgcttcctt tctcaataga
    12901 tagatttgat acaaacgaaa ttctccataa tctaattgtc catctacatt ccattcgcta
    12961 aagcgccact ctcgcacgga atccttattt ggttccaaac gttcgattgt ttctgcagag
    13021 agaatccact cgtatggaaa atcatcaaaa aacaaggccc actcaataag atgatctgtt
    13081 actgttcctc tttttaaatg acggattgcc atgatctggt caattgtctg accattcata
    13141 aacatctgct tggtgatcat catgctttga ttgaaatttt gctgtaataa tgaatcgatc
    13201 aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag gtggatacat
    13261 ctgctttgtt gcagataaac ctctgactca tcattgtttt ttgccagctg ataaggaagc
    13321 agccctgttc gctcatttcc agataattgg ttagctaaaa aatctgctgc ttcttctggt
    13381 atctccgaaa acagctgcac caaactctca tacgcagtat caattaaaat ttcacgtggc
    13441 aattggcttt cactaagcca tttttttagt tggaaagtat aaaaaggact tgtctctgct
    13501 ggaagatagt cttgaatacc cgttgccaga ttagaaatta cttgaacagc aaatttaatg
    13561 agccgccaac tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc agtatgttct
    13621 atttgatgtt cggacaacat atcagcccct gcaggcgtta gctttgcttc attttcattt
    13681 atttcgatcc aaccttgctg cgcaattttt tgcaaaattt gataaaactt atcctgcttc
    13741 aattctggaa aactgttatg gataaacaaa agttcgtggg taaatccata aataagtact
    13801 gaagtcgttc gttttcccac aaggagttgg taaagagaac taatccgcag cttgtgtcct
    13861 gatgaaaata gcgataaaat aaaaacaatc atattattct ccttaagtta ggatggttag
    13921 aaatgtcact actatgtaaa cttgtacctg aacgctgtat tgcatgcggt ctttgtcaaa
    13981 tcgaagcgcc agatattttc gattatgatg aagaaggaat cgttttattt gcccaagaac
    14041 cagaggctca tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa aaagcgtatc
    14101 aaaaatgtcc agttcgagcg attttattag aaaataaaat ataaatataa acgaaacttt
    14161 aaaaacgtac actaagttct ctaaacttga aaataagccg ggatctgttt ctcttgttcc
    14221 gtgaaatccc gacttatttt gaaagattca tttgctatct atagttaatt gactagtttt
    14281 ttcacggacc ctataggacc catttatatt atttcaactt tttcagccag ttttccaatg
    14341 tattgacgat cacagtttgc tggtctgcgt tagaaatttc tgcctctcca tctccttttt
    14401 gctcaccata gctgccaaaa cctccatgat ttccgccatt gattgataca tactctgttc
    14461 tttccggtaa atactgctta gcttcttgat aagcttcctg attcaatact ttatcttttg
    14521 tcgccgtgat cgataaaaca ggaacagctg attctgtcaa tgttcctttt ttgtcgggat
    14581 aacttgccat gaagaatacc ccttttaaat gtttgtctga atgattctta ttgacataac
    14641 gacttgccat tacgccacct aatgaatggc caccgatcac ataatcggaa tttggaaaat
    14701 cttctttgac cttatccgct ttgtttccgc ccatcactgc tagatcaagt ggcattttga
    14761 caatagcgac tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta taactttctg
    14821 gctctacgag agcaccagga taaaaaataa gtaatggaga ctttccatga ttctttttct
    14881 ctccttcaaa aaataagtaa tcttctttgt tttttccttc ttttaccaac acttgcgctt
    14941 ctgacgatgg ttcgtacgta ttttgctgta tatagtaata accggctcca ccgagtaatc
    15001 ctaaaatgat gactaagcca attagtattc gtctaaatat tttcactttc tcccaccttt
    15061 tctatctgag tgaccccttt ttcttaatca agtatgatta taccttaaaa aaataagaga
    15121 gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa tatccatttg aatatccgcc
    15181 ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg cataaatttc
    15241 cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca taaagtcagg
    15301 agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag caaaagtagc
    15361 gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg tccccaatag
    15421 atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt caaattcttc
    15481 ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat catctttctt
    15541 gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg tgatttccaa
    15601 acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc ccacgacagc
    15661 ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc caaaatcaaa
    15721 tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg ctgcatcttt
    15781 cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga aatcgaattc
    15841 gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag gagctgcatc
    15901 aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc ctggtcctgt
    15961 caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag catctggcgc
    16021 aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac gccataaatt
    16081 ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc cagcatcaat
    16141 caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt gggatccgcg
    16201 accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc aattattttc
    16261 gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat ctccagagcc
    16321 aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc ctagcttttc
    16381 actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg gatgtccttg
    16441 tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca atgattctaa
    16501 tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct cctaatatgt
    16561 taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata atgacgtttt
    16621 aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa aaacactcac
    16681 tgaatggagc tattgacttg tccacgtgct tatcgatagc cgtttatcgg tttcttcatt
    16741 cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc tacacctagg
    16801 atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt ccaccacact
    16861 gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt tggtgtaatt
    16921 aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt accgacgccg
    16981 gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc ggaagttgaa
    17041 ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa tacacttgaa
    17101 gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat taatccattg
    17161 ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc gatcaaatca
    17221 gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata agtgattgct
    17281 actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca gtcacctaat
    17341 ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa ccagtttgtc
    17401 aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa actgatgatc
    17461 atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt cgtgattact
    17521 tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg ttggttgtat
    17581 actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac atatacaata
    17641 gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc acccatcgaa
    17701 atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga agctagacag
    17761 cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa gcctacagcc
    17821 atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat tccgtaagta
    17881 gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc ttttcctttg
    17941 aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt gataatacct
    18001 aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg tgtttcttta
    18061 gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa aatatcccac
    18121 agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt gcctgcatcg
    18181 tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac tgcaataata
    18241 ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt ttgttccatc
    18301 ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt attttatcaa
    18361 aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt caaaaagttc
    18421 gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt tttcattgta
    18481 gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag aatttcacat
    18541 ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa caaacaaata
    18601 aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc aagaaatttc
    18661 ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga tgttgttttc
    18721 gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc acgatgaatc
    18781 ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa gacatgccca
    18841 gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata acgaaataat
    18901 ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct aaaaatttgt
    18961 ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat gtaaagatcg
    19021 ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa cctgtagtca
    19081 gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga ccgaataaga
    19141 acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta aaagccggca
    19201 tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc gctactgcaa
    19261 ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact cctcttcagc
    19321 gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa aatagagtta
    19381 gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga ctatactgtc
    19441 ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg actataacca
    19501 ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt gtttacatat
    19561 tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag taagtgttca
    19621 ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt aatccccagg
    19681 acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt gcacaggaag
    19741 accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga tcgtcaattc
    19801 cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag ctggagatgt
    19861 ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt ttgtagcgat
    19921 cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg tcaggcgctg
    19981 agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat ccagtcgacc
    20041 tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt ttcttccttt
    20101 gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt aaccgtattc
    20161 ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa cttttatccc
    20221 taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga attcttctgc
    20281 ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct ccattacaat
    20341 tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc ttcaagtgaa
    20401 atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa ataatctaag
    20461 aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct acctttttct
    20521 tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac tcctcgaatc
    20581 tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa ggtttcgacg
    20641 gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa gggagccagc
    20701 tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa agctgagtgc
    20761 gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc tgcttcgctg
    20821 agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac aataaaaaga
    20881 agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg gataaagcat
    20941 aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca tcaattctag
    21001 caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa aagaatcgaa
    21061 agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc gttcttcgat
    21121 cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct tacgttctaa
    21181 gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct tcaacggctt
    21241 ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa gacttcgttc
    21301 ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt ctaaaagctg
    21361 agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg tgccgaattc
    21421 ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca tcactaagta
    21481 ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca tatattgctc
    21541 cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa tcagatgaag
    21601 cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg tcaactcatt
    21661 cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt gatttcagtt
    21721 gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg aaagcccata
    21781 ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg atcgttcgtt
    21841 gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt aaagaaaaaa
    21901 agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga agttttacct
    21961 gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc tttccggtaa
    22021 tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg cttataaaca
    22081 tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat atccgcgtga
    22141 cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg tgtggcaaag
    22201 ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg tactaactgc
    22261 ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt gacaaataca
    22321 tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta gcgttccagc
    22381 cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt tccaactgtc
    22441 tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa ttcactgacg
    22501 cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc taacgttttt
    22561 gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt acttggtaat
    22621 ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt gatacgctct
    22681 tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac agttgctgtc
    22741 gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg atcgacatcc
    22801 tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa atcacgttca
    22861 tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg gagataatcg
    22921 ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag ataccgcttc
    22981 attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac actcagacgg
    23041 actggttt
//
LOCUS       DAFGZE010000042        22859 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.043, whole genome shotgun sequence.
ACCESSION   DAFGZE010000042 DAFGZE010000000
VERSION     DAFGZE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.043"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            116..670
                     /locus_tag="LTU54_001778"
     CDS             116..670
                     /locus_tag="LTU54_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8296492.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(843..1316)
                     /locus_tag="LTU54_001779"
     CDS             complement(843..1316)
                     /locus_tag="LTU54_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8296493.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1328..1864
                     /locus_tag="LTU54_001780"
     CDS             1328..1864
                     /locus_tag="LTU54_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8296494.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1904..2275
                     /locus_tag="LTU54_001781"
     CDS             1904..2275
                     /locus_tag="LTU54_001781"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8296495.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2559..3830
                     /locus_tag="LTU54_001782"
     CDS             2559..3830
                     /locus_tag="LTU54_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBL8296496.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4075..5100
                     /locus_tag="LTU54_001783"
     CDS             4075..5100
                     /locus_tag="LTU54_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296497.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5394..6503
                     /locus_tag="LTU54_001784"
     CDS             5394..6503
                     /locus_tag="LTU54_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL8296498.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6586..7482
                     /locus_tag="LTU54_001785"
                     /pseudo
     CDS             6586..7482
                     /locus_tag="LTU54_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7498..8322
                     /locus_tag="LTU54_001786"
     CDS             7498..8322
                     /locus_tag="LTU54_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296499.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8354..9664
                     /locus_tag="LTU54_001787"
     CDS             8354..9664
                     /locus_tag="LTU54_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HBL8296500.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9812..11071
                     /locus_tag="LTU54_001788"
     CDS             9812..11071
                     /locus_tag="LTU54_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL8296501.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11081..11953
                     /locus_tag="LTU54_001789"
     CDS             11081..11953
                     /locus_tag="LTU54_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8296502.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11965..12795
                     /locus_tag="LTU54_001790"
     CDS             11965..12795
                     /locus_tag="LTU54_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296503.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12835..15018
                     /locus_tag="LTU54_001791"
     CDS             12835..15018
                     /locus_tag="LTU54_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL8296504.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15079..16299
                     /locus_tag="LTU54_001792"
     CDS             15079..16299
                     /locus_tag="LTU54_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBL8296505.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16296..17756
                     /gene="gtfA"
                     /locus_tag="LTU54_001793"
     CDS             16296..17756
                     /gene="gtfA"
                     /locus_tag="LTU54_001793"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBL8296506.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17833..18939
                     /locus_tag="LTU54_001794"
                     /pseudo
     CDS             17833..18939
                     /locus_tag="LTU54_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19261..19470
                     /locus_tag="LTU54_001795"
     CDS             19261..19470
                     /locus_tag="LTU54_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL8296507.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19463..19612
                     /locus_tag="LTU54_001796"
     CDS             19463..19612
                     /locus_tag="LTU54_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBL8296508.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19766..20431
                     /locus_tag="LTU54_001797"
     CDS             19766..20431
                     /locus_tag="LTU54_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8296509.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20715..21800)
                     /gene="xerS"
                     /locus_tag="LTU54_001798"
     CDS             complement(20715..21800)
                     /gene="xerS"
                     /locus_tag="LTU54_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HBL8296510.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22134..22367
                     /locus_tag="LTU54_001799"
     CDS             22134..22367
                     /locus_tag="LTU54_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HBL8296511.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22364..22771
                     /locus_tag="LTU54_001800"
     CDS             22364..22771
                     /locus_tag="LTU54_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HBL8296512.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7813 a         3425 c         4382 g         7239 t
ORIGIN      
        1 aaactaaatc ccttgatttt attgactttc aaaagtttat atatataagt taatgattat
       61 ggtaaaatat atacataatc attaactcag aatattttta agaagggaaa caatagtgag
      121 ctataatgta caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag
      181 aagaacaagt aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt
      241 acgtatgtca gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat
      301 tattgttgaa cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga
      361 aattttatta tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca
      421 aggtcgacat ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt
      481 aggacgagat gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa
      541 gaaaactaga gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac
      601 aaaaaggtac attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact
      661 aggtgtataa tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat
      721 ctagaacgat tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt
      781 ataggttcgt gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga
      841 aattaaaaaa agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat
      901 actgataact caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg
      961 accatacttt tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct
     1021 gcaaaacgaa ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc
     1081 atagtggttt taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca
     1141 gattcaaaga gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag
     1201 ttattctctt taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta
     1261 ttttcttctg atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt
     1321 agatattatg cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac
     1381 atttgacttt tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga
     1441 cgatcatacc atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact
     1501 gacttttttt gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg
     1561 aacgatacta aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac
     1621 acaaggcgtt tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga
     1681 agggccagat gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga
     1741 ttatttcccg tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc
     1801 cattcaacta cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg
     1861 gtagcacaaa gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa
     1921 tcaagacact cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc
     1981 cattgaaggg attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt
     2041 acaacgcatc gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg
     2101 tgaatttttt gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac
     2161 aggggaagga aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat
     2221 tcctttattt ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc
     2281 taatcaaatc catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac
     2341 gcatcaaatc cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca
     2401 ttttcgacta ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct
     2461 ctttctaggt aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact
     2521 atttcaaaat aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga
     2581 aacaacttaa aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg
     2641 tatctggttc aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc
     2701 aactgaatga cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag
     2761 aggttgatga gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg
     2821 gcagtgcgcg ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt
     2881 tttcatgttt tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta
     2941 gtggaatgtg ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc
     3001 tttatttttc ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag
     3061 ttttgtctgg aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg
     3121 ttaattcagc agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg
     3181 ttgttggtgt acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg
     3241 tttatattga tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc
     3301 gtttatttgt aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc
     3361 attatggtca tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg
     3421 ttattcaaga acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta
     3481 aatcgcattg gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat
     3541 ttactgcaat tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa
     3601 ataagaataa acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg
     3661 cagaattaat ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga
     3721 ttgcttttca ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac
     3781 tattagaggg agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg
     3841 tgaaataagt atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta
     3901 gcagttcctc gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat
     3961 tttttcttgt ttttaattat gattacagtg cctattttta acgaaatttg cttattttca
     4021 aaatgtatga ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta
     4081 ggaattaggg atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta
     4141 aatgaggatc cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt
     4201 aatttttatc attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta
     4261 ataacgacag tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga
     4321 gggattcaag tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca
     4381 gaaacaccgt tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag
     4441 gtgctatctt ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat
     4501 gaccctagta ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat
     4561 cagcatttag atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga
     4621 cgtgttcgtt tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata
     4681 gcctatgaga cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa
     4741 ggctggacca cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa
     4801 gataagttgc cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt
     4861 tcacttcaaa aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat
     4921 attgaagttg ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa
     4981 attggaaaac tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg
     5041 atcaaagtga ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa
     5101 aaaagtaaac atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa
     5161 atctcgacaa aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca
     5221 aaacaggagg aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt
     5281 taactgggac attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag
     5341 taactttaag ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag
     5401 atgaatttga aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc
     5461 aatattgggt taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca
     5521 tgcactctag cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg
     5581 ttatcaaaga aaatgatatt gacatgggaa actttactga aggaattgct gatttctata
     5641 cgcttgaaga cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca
     5701 acaaagaatt atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa
     5761 cactcagaca agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat
     5821 cacatattgg aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat
     5881 ttgccgatga caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt
     5941 tttatcgaga tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc
     6001 ctgataagtt aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt
     6061 ttgcaccggg atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac
     6121 ctaagggaaa agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta
     6181 caaaataccc agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata
     6241 aaatccaagc ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg
     6301 ctgcttcttc agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc
     6361 ctcgtccata caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc
     6421 gtattctgtc tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca
     6481 atgcgatatt gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct
     6541 atttcgtctg tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt
     6601 tttggaaaat atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca
     6661 ttaggattaa ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa
     6721 aaaacggtag ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt
     6781 gcagacggtg aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata
     6841 cctagtattg ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga
     6901 ttgtcgattt atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca
     6961 atgatttggc gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa
     7021 ataggtctcg atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc
     7081 attgtaggag tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa
     7141 gaaattccta aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag
     7201 tttttcaaca ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca
     7261 gttatcaatg ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc
     7321 gccatgttta aatcacaatc attagcctat ttattctata aacattcatt tattttaaat
     7381 gacaagggat atggttcggc aatcgttatg ttccttttag tgattatatt aattattact
     7441 gcaattcaaa tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg
     7501 aaaaataaat ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca
     7561 atggcattac cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc
     7621 caaattccac ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt
     7681 gatttattac cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg
     7741 attggctcgg ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca
     7801 gggaaaaata tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt
     7861 attactccac agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta
     7921 atattaccag gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt
     7981 ttacctgatg aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt
     8041 ttcaaaatta tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg
     8101 ttgtttgcat ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg
     8161 cctttagctc cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta
     8221 atggcaggtt cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga
     8281 aaattcattg aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt
     8341 ggagagtaag tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa
     8401 aaacgtatta ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta
     8461 cgatattgat gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa
     8521 ttcgaatgaa aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga
     8581 ggctgatttt gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga
     8641 ttttgatatt cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg
     8701 tgggattttt agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga
     8761 agaagtttgt ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat
     8821 gggtgtttta aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg
     8881 cgtaccagaa ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca
     8941 atggaaaatt gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga
     9001 agatttctat ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc
     9061 tgtacgtttt gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa
     9121 tgcagaatac catccatact ttattaaatc aaaataccca gaattgattg acgaattagg
     9181 gattccgttg gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat
     9241 gcgagaggac attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc
     9301 atatatcatg gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa
     9361 taaaggctta atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga
     9421 taaaaatggc gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa
     9481 tcggacgaat atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc
     9541 aaaaatttat caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga
     9601 tatcaaaaat ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa
     9661 ttaaaaagaa gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt
     9721 tagtaaaaat attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa
     9781 cgcattcaaa ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc
     9841 ttgtatagca ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc
     9901 aagcggtaag gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca
     9961 ggagattgct aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt
    10021 accgaatgct ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat
    10081 taatctttat cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga
    10141 cttaaccgaa gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat
    10201 tgaggatcgt gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa
    10261 aactgctttt gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat
    10321 agtagctgag attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg
    10381 gactttaaat ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc
    10441 gaacaatgtt tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca
    10501 aaaagatttt aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca
    10561 aggtgccggt tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc
    10621 gaatggttca tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac
    10681 ttttcctttt ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc
    10741 cttatcagtt tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat
    10801 gacaacacct gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga
    10861 gggcgttatc gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac
    10921 tgatcgtcat ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt
    10981 aacaacaaat tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt
    11041 ctttaatcca atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt
    11101 aaatcgttac tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt
    11161 ctactttcca atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta
    11221 taattttgat tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat
    11281 caaatcgatt ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt
    11341 tggtattttg attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc
    11401 atggttcttt tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat
    11461 ttttaactat ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag
    11521 tcttttagga acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg
    11581 aattgccatg ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat
    11641 tgaagctgct agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac
    11701 ctacttatta ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc
    11761 atttgatcaa atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg
    11821 gttacttgtt tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc
    11881 tttagtctta tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa
    11941 atttgaaatc taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac
    12001 ttgcttttat tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt
    12061 tcatttaaaa caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact
    12121 ttggaaaatt tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca
    12181 atcatcacaa tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca
    12241 ctagcaagaa aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta
    12301 ggaatttttg ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt
    12361 ggtttagcaa atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca
    12421 ctatttttat atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct
    12481 gaaattgatg gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa
    12541 ccaatgcatg cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta
    12601 ccattattaa ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat
    12661 tatacgggac aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt
    12721 gtcacgatta cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat
    12781 ggagcagtga aataaccaat aaattattct cattataaag gagttcataa acttatgatt
    12841 atatttgaca atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta
    12901 ggaatcgaag aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat
    12961 tctggtgggt atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg
    13021 caagatcgtt tattatcttt agacacatta ccattagaat ttccaggata tggacatggt
    13081 gattttagag aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt
    13141 cgttatgata gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta
    13201 tatacggaag atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt
    13261 ggattgactt tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct
    13321 acgcaattag taaataatag tgatgaaaca gtggagatta accgcttagc tagtcaatca
    13381 attgattttc caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa
    13441 cgtcaaatga ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga
    13501 tcaagcagcc atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt
    13561 caaggagagg catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa
    13621 aaagatccat ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg
    13681 caactaaaaa ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa
    13741 ggtctgaata cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga
    13801 aactatcgtt taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat
    13861 tttaacgaag aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg
    13921 tttgttttag atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat
    13981 tgggttgaaa cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat
    14041 gataacggga tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa
    14101 ctatttcgag cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt
    14161 cgaaatcaat atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa
    14221 atgacggcga tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat
    14281 atgacagaag tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt
    14341 tatatgttag ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta
    14401 tttgaaagtt gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca
    14461 caaacttgga cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc
    14521 agcttagtgt atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa
    14581 acaggaagag aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt
    14641 tatgagctag atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt
    14701 gccttttata aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt
    14761 ccgtttgaag gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg
    14821 gtcttttatt tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca
    14881 ggattagcag aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg
    14941 atgaatctcg gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt
    15001 tatttgaaac agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc
    15061 attccaaacg aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg
    15121 aattgggaaa atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat
    15181 aaaggactag gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat
    15241 ggcatctttg aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat
    15301 cagtcgggca ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat
    15361 agtatgacgg tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa
    15421 gatggctatt attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact
    15481 ggtgaatggt ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg
    15541 aatcaattag ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa
    15601 ccacgttcaa ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg
    15661 ttggcgacgc tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta
    15721 ggaatgacca acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct
    15781 cagtataaac gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat
    15841 aaacgaagtc gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga
    15901 ttctcaactt ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca
    15961 gaagctcagt taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta
    16021 agaaaaaata gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat
    16081 aaaaatgaac atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc
    16141 ttaaacgtta cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta
    16201 atcaataatt atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt
    16261 gaaagtattg tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc
    16321 tcatcaccta tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca
    16381 aacatttaca aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg
    16441 atcgaggctt tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag
    16501 atgtggaagc attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt
    16561 ctcgtgaatc aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag
    16621 aaatgtttat ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg
    16681 atttaatcta caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta
    16741 cgacagaaga ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag
    16801 aagttgtgaa agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt
    16861 taattcgatt agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg
    16921 tggaaccaga tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg
    16981 tcgaattatt accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg
    17041 attattatat ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa
    17101 aatcagaacg tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg
    17161 atacgcatga tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg
    17221 attacacctc tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg
    17281 ccaattacaa taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg
    17341 ataatgaccg tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac
    17401 aaatttatta cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga
    17461 aagaagggcg taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc
    17521 aacggccagt gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt
    17581 ttgatttgga tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac
    17641 gtaaaaataa aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat
    17701 ttatcattga gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta
    17761 ttccatagaa caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa
    17821 aaggactcat acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc
    17881 ataaaattag gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat
    17941 cgtaaaaaac tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa
    18001 aaagaaagtt tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc
    18061 gatgtgtcat cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa
    18121 gtgttagaag aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc
    18181 aagcgaaatc atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat
    18241 aagtgggcga ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat
    18301 atggacgata ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat
    18361 ctcaatcaac taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg
    18421 tattaggact ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg
    18481 attaaaaaaa acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa
    18541 attctacgag acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga
    18601 ctagtcactg ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac
    18661 gtctcttcaa aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc
    18721 aagcacatca aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg
    18781 cagaatcaat tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag
    18841 acgaaaacgg aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag
    18901 ccctttgaat gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt
    18961 tacttttggt tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc
    19021 gtcagcacag ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat
    19081 gttttatgca acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt
    19141 attaaggagc aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag
    19201 gaacgctact atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa
    19261 atggtggtta taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag
    19321 agaatcatac gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat
    19381 agaagattag gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag
    19441 gtagaggagg attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca
    19501 ttttactgct agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga
    19561 ggggttaaaa cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa
    19621 aagaagcaga aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc
    19681 taccttatta tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca
    19741 aaaaaacttt tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca
    19801 aaagtggcaa aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg
    19861 gatttttcag atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc
    19921 aaaagttatt ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga
    19981 atttacaaaa gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat
    20041 ttgtatgtgg ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg
    20101 gaaagatatt gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag
    20161 agccaaaacc tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact
    20221 agacgacaat acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg
    20281 catgtcgttt gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat
    20341 tatgagaaga catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca
    20401 tagtcatgct tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt
    20461 aaaacatgct gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt
    20521 gagagagata acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc
    20581 gacacctatt tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga
    20641 aacacactag aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta
    20701 tttatacatt atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg
    20761 gtatataaag ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat
    20821 gaatttgtct gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact
    20881 tgaattctaa agccttttga atattttgct accatccgtt cgattgcaat accggatata
    20941 cgcttggctt ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta
    21001 tatcgttctt tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata
    21061 ttgacagcat ctcttttatt tccttttcta gttactacca cattattctt atctaaagat
    21121 aggtcatcta aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt
    21181 gcattgatcg ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc
    21241 aaagtatttt tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga
    21301 aataattttt tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt
    21361 gctacttgat ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt
    21421 ttcgaaagat atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta
    21481 gatagaccta agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt
    21541 tcttttgtgc gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt
    21601 cttatcgagg tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag
    21661 agcttataat tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga
    21721 tcttgaacaa aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga
    21781 tatatttctg tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc
    21841 tataattgga ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt
    21901 aatttgtttc aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta
    21961 tacataaata gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta
    22021 attatataga aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt
    22081 aaaatcaaca agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa
    22141 ttaaagaaag aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt
    22201 ttcttaaaga aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc
    22261 gagcggcaat tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag
    22321 cattaactga atatgatact acttttagag agcttgtcga taaatgagta ttcattacct
    22381 agaagccaaa gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga
    22441 accccttggt gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc
    22501 agcttttggt gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca
    22561 attgataaag aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt
    22621 tgtctttttg aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt
    22681 agcggtatat attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa
    22741 cactataaag acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt
    22801 attagctgct atagcgaaat aattgaagta aattacaaat agctataatt caaatccaa
//
LOCUS       DAFGZE010000043        22298 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.044, whole genome shotgun sequence.
ACCESSION   DAFGZE010000043 DAFGZE010000000
VERSION     DAFGZE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.044"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            2..400
                     /locus_tag="LTU54_001801"
     CDS             2..400
                     /locus_tag="LTU54_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBL8296513.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="LTU54_001802"
     CDS             384..1079
                     /locus_tag="LTU54_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBL8296514.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="LTU54_001803"
     CDS             complement(1673..2560)
                     /locus_tag="LTU54_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL8296515.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="LTU54_001804"
     CDS             2745..3584
                     /locus_tag="LTU54_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL8296516.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="LTU54_001805"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="LTU54_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HBL8296517.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="LTU54_001806"
     CDS             5052..5408
                     /locus_tag="LTU54_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296518.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="LTU54_001807"
     CDS             5411..6568
                     /locus_tag="LTU54_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296519.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="LTU54_001808"
     CDS             complement(6594..7034)
                     /locus_tag="LTU54_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HBL8296520.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="LTU54_001809"
     CDS             7194..7700
                     /locus_tag="LTU54_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL8296521.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9781
                     /locus_tag="LTU54_001810"
     CDS             7868..9781
                     /locus_tag="LTU54_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HBL8296522.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9786..10787
                     /locus_tag="LTU54_001811"
     CDS             9786..10787
                     /locus_tag="LTU54_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296523.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10989..11195)
                     /locus_tag="LTU54_001812"
     CDS             complement(10989..11195)
                     /locus_tag="LTU54_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HBL8296524.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11841..12689)
                     /locus_tag="LTU54_001813"
     CDS             complement(11841..12689)
                     /locus_tag="LTU54_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL8296525.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12688..13755
                     /gene="gnd"
                     /locus_tag="LTU54_001814"
     CDS             12688..13755
                     /gene="gnd"
                     /locus_tag="LTU54_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HBL8296526.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13757..14758
                     /locus_tag="LTU54_001815"
     CDS             13757..14758
                     /locus_tag="LTU54_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBL8296527.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAEKNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14755..15039
                     /locus_tag="LTU54_001816"
     CDS             14755..15039
                     /locus_tag="LTU54_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBL8296528.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15072..15869
                     /locus_tag="LTU54_001817"
     CDS             15072..15869
                     /locus_tag="LTU54_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL8296529.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15883..16716
                     /locus_tag="LTU54_001818"
     CDS             15883..16716
                     /locus_tag="LTU54_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296530.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16734..17591
                     /locus_tag="LTU54_001819"
     CDS             16734..17591
                     /locus_tag="LTU54_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8296531.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMDQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17613..18122
                     /locus_tag="LTU54_001820"
     CDS             17613..18122
                     /locus_tag="LTU54_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296532.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18159..18560
                     /locus_tag="LTU54_001821"
     CDS             18159..18560
                     /locus_tag="LTU54_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296533.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18672..19025
                     /locus_tag="LTU54_001822"
     CDS             18672..19025
                     /locus_tag="LTU54_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296534.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19059..19502
                     /locus_tag="LTU54_001823"
     CDS             19059..19502
                     /locus_tag="LTU54_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL8296535.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19519..20604
                     /gene="uxuA"
                     /locus_tag="LTU54_001824"
     CDS             19519..20604
                     /gene="uxuA"
                     /locus_tag="LTU54_001824"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL8296536.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20597..21310
                     /locus_tag="LTU54_001825"
     CDS             20597..21310
                     /locus_tag="LTU54_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8296537.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21517..21666)
                     /locus_tag="LTU54_001826"
     CDS             complement(21517..21666)
                     /locus_tag="LTU54_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296538.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22062..22262
                     /locus_tag="LTU54_001827"
     CDS             22062..22262
                     /locus_tag="LTU54_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL8296539.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7680 a         3427 c         4619 g         6572 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtcttag ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 cccatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc
     9721 aggtggattt ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta
     9781 aatgaatgaa atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt
     9841 tagtcactgt atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa
     9901 aacaatttga cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa
     9961 aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata
    10021 atatggatat ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc
    10081 atggaggcgc atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat
    10141 gttcagacac gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac
    10201 atcctacacc agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag
    10261 atcaagcaga ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg
    10321 gagaatattt gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga
    10381 ctttggagaa caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg
    10441 cacttactca agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt
    10501 actttgataa taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac
    10561 atgcggatcg ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga
    10621 aacaaatgga agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag
    10681 attatgcgga gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac
    10741 aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc
    10801 aaaagaggtt gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag
    10861 cttttcatgg tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca
    10921 ccttttattc gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat
    10981 tttataagtc agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg
    11041 gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt
    11101 ccgttgttat tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta
    11161 gcgaaaaaaa gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt
    11221 gtatgataaa tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat
    11281 gatcatctag cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat
    11341 cagataggta caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat
    11401 aatagaaaag atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa
    11461 cttttttatt ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta
    11521 aaatgaggcc aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa
    11581 ttttttagaa aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact
    11641 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
    11701 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
    11761 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
    11821 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
    11881 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
    11941 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
    12001 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
    12061 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
    12121 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
    12181 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
    12241 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
    12301 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
    12361 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
    12421 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
    12481 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
    12541 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
    12601 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
    12661 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
    12721 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
    12781 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
    12841 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
    12901 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
    12961 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
    13021 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
    13081 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
    13141 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
    13201 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
    13261 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
    13321 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
    13381 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
    13441 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
    13501 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
    13561 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
    13621 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
    13681 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
    13741 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
    13801 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
    13861 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
    13921 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
    13981 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
    14041 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaaaaa
    14101 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
    14161 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
    14221 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
    14281 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
    14341 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
    14401 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
    14461 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
    14521 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
    14581 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
    14641 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
    14701 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
    14761 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
    14821 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
    14881 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
    14941 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
    15001 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
    15061 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
    15121 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
    15181 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
    15241 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
    15301 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
    15361 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
    15421 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
    15481 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgtggcag
    15541 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
    15601 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
    15661 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
    15721 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
    15781 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
    15841 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
    15901 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
    15961 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
    16021 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
    16081 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
    16141 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
    16201 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
    16261 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
    16321 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
    16381 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
    16441 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
    16501 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
    16561 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
    16621 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
    16681 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
    16741 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
    16801 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
    16861 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
    16921 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
    16981 gtgtgattac tggcctaatg tcttctatgg atcagcaaag agcagaagaa gagtatgaag
    17041 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
    17101 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
    17161 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
    17221 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
    17281 gtaatgctac aggcctaaag caaaacactg gtgttcagac agcgattgaa atgctatcag
    17341 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
    17401 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
    17461 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
    17521 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
    17581 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
    17641 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
    17701 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
    17761 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
    17821 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
    17881 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
    17941 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
    18001 atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag
    18061 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
    18121 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
    18181 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
    18241 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
    18301 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
    18361 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
    18421 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
    18481 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat
    18541 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
    18601 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
    18661 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
    18721 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
    18781 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
    18841 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
    18901 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
    18961 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
    19021 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
    19081 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
    19141 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
    19201 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
    19261 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
    19321 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
    19381 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
    19441 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
    19501 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
    19561 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
    19621 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
    19681 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
    19741 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
    19801 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
    19861 actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc
    19921 gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc
    19981 aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat
    20041 gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt
    20101 ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa
    20161 ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca
    20221 gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa
    20281 aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga
    20341 aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc
    20401 tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata
    20461 cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat
    20521 gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa
    20581 ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt
    20641 attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta
    20701 tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga
    20761 acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa
    20821 tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga
    20881 agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac
    20941 acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat
    21001 catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg
    21061 ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat
    21121 cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt
    21181 gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa
    21241 agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt
    21301 gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga
    21361 gggaacagat gaaaaagaag taatttttta ttttgaagta tctaccttat ttgtagtgaa
    21421 caatttagaa caaacacaaa acgcatctac tgttatctac cagtagatgc gtttaaagag
    21481 aagaacgaga atcgtccata tgagggtttc cactccttat gcagattttt ttaattcttg
    21541 cactgcttgt tcgatcgtaa cacctgtcgc tacatgattg gtattttctt tgtcaaaaac
    21601 gacaaataga ttcaaatctg tatccaaggt tactcgatat tgtatatctt tgttatagaa
    21661 tgtcatagcc attctccttt ctttaaatac ttgctgaaag gcaacgaacg cgttgataac
    21721 gaaattccat ttataagtat accaccaatt gaaaggtttt catagttaag aaattttaaa
    21781 gtcaaaagtt gcagataaaa gcaaataaac aagaaaacaa aaagtgaatc aatcgcggtt
    21841 attcaacttt ttcttcgatt cgaagacaat agaagacaat gaattttata tttcattgca
    21901 ttatccttgt gttattagaa aaagcatgat atattaaatg agtaatctat tttgaaacgt
    21961 aatttgagcg atatatctac atagataaaa ctccttttgc ctatcaaata tttattgcaa
    22021 cgaaaaaacg tacattgata cgtatcagga ggaatttatt tatgaataac ggtacagtaa
    22081 aatggttcaa cgcagacaaa ggttttggct ttatcactgg tgaagatgga aatgatgtat
    22141 ttgcacattt ttcagctatc caaggtgacg gcttcaaaac tttagaagaa ggccaagcag
    22201 tgtcttacga tgttgaagat ggtcaacgtg gccctcaagc aacaaacatt gttaaagctt
    22261 aataaaaatt gacttttaac cgtcttttcg aaagaaaa
//
LOCUS       DAFGZE010000044        22170 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.045, whole genome shotgun sequence.
ACCESSION   DAFGZE010000044 DAFGZE010000000
VERSION     DAFGZE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.045"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            240..2075
                     /gene="tdc"
                     /locus_tag="LTU54_001828"
     CDS             240..2075
                     /gene="tdc"
                     /locus_tag="LTU54_001828"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL8296540.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2380..3858
                     /locus_tag="LTU54_001829"
     CDS             2380..3858
                     /locus_tag="LTU54_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296541.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3824..5155
                     /locus_tag="LTU54_001830"
     CDS             3824..5155
                     /locus_tag="LTU54_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296542.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5269..7821
                     /locus_tag="LTU54_001831"
     CDS             5269..7821
                     /locus_tag="LTU54_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBL8296543.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7828..9267)
                     /locus_tag="LTU54_001832"
     CDS             complement(7828..9267)
                     /locus_tag="LTU54_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296544.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9613..9975
                     /locus_tag="LTU54_001833"
     CDS             9613..9975
                     /locus_tag="LTU54_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBL8296545.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10162..11019)
                     /locus_tag="LTU54_001834"
     CDS             complement(10162..11019)
                     /locus_tag="LTU54_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8296546.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11139..12050
                     /locus_tag="LTU54_001835"
     CDS             11139..12050
                     /locus_tag="LTU54_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBL8296547.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12057..13703
                     /gene="mdcA"
                     /locus_tag="LTU54_001836"
     CDS             12057..13703
                     /gene="mdcA"
                     /locus_tag="LTU54_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HBL8296548.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            13805..14104
                     /locus_tag="LTU54_001837"
     CDS             13805..14104
                     /locus_tag="LTU54_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HBL8296549.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14094..15770
                     /gene="mdcD"
                     /locus_tag="LTU54_001838"
     CDS             14094..15770
                     /gene="mdcD"
                     /locus_tag="LTU54_001838"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HBL8296550.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15774..16367
                     /locus_tag="LTU54_001839"
     CDS             15774..16367
                     /locus_tag="LTU54_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HBL8296551.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16391..17467
                     /locus_tag="LTU54_001840"
     CDS             16391..17467
                     /locus_tag="LTU54_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBL8296552.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17492..18361
                     /gene="mdcB"
                     /locus_tag="LTU54_001841"
     CDS             17492..18361
                     /gene="mdcB"
                     /locus_tag="LTU54_001841"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HBL8296553.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18472..19236
                     /locus_tag="LTU54_001842"
     CDS             18472..19236
                     /locus_tag="LTU54_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HBL8296554.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19725..21818
                     /locus_tag="LTU54_001843"
     CDS             19725..21818
                     /locus_tag="LTU54_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL8296555.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21843..22145
                     /locus_tag="LTU54_001844"
     CDS             21843..22145
                     /locus_tag="LTU54_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296556.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7177 a         3786 c         4724 g         6483 t
ORIGIN      
        1 tgtttgttcg ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc
       61 gcttttttgc gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc
      121 tacgtggatt tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg
      181 ttacagataa acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa
      241 tgaaagatat ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt
      301 ataaaatgtt gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc
      361 cttcagatat gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc
      421 gcgaccatat gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc
      481 catggcattc agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt
      541 tattagctta caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta
      601 tggcaacttc gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga
      661 cagacggatg gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt
      721 acgcccgttg catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga
      781 aaaaaatgtc tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg
      841 aaagctttac ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa
      901 atatccaaag attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg
      961 cgttagatat ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt
     1021 atcgaatgga tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc
     1081 caattctagg tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag
     1141 ataaaatcgt ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg
     1201 ttgatgccgc atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg
     1261 tgccgtatgc ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa
     1321 cgattgacaa agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga
     1381 tagatcctca taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata
     1441 aaaatatgag aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg
     1501 ctccagatat gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg
     1561 cagtctggac agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg
     1621 gggcttcaat cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg
     1681 ttaaaggaaa aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt
     1741 gggtattcaa agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa
     1801 tgttcgatcg gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc
     1861 atactacatt tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg
     1921 gtctaacgaa agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca
     1981 tgactcctta tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag
     2041 caatggaaaa gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa
     2101 caagagacat atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct
     2161 gtttttcata tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc
     2221 gttcgtttca tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac
     2281 tgggttttgt actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga
     2341 aaacaaaaac atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca
     2401 aagcaaatca cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga
     2461 aatattccag atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta
     2521 ttattgtacg cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa
     2581 aaaggtggtc cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc
     2641 tcatggctgt tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct
     2701 ccgttgtttg gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta
     2761 gttgtcattt tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca
     2821 aaaatcggcg gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc
     2881 ttattagggt ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt
     2941 ttttcatggg acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg
     3001 ccgatcatgt ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa
     3061 aatccagtta aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc
     3121 aatattttga atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat
     3181 aatatcgcgc aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg
     3241 aatctgttta gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca
     3301 ggaccagcaa agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat
     3361 ttttggaaga cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt
     3421 atctcaatct ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg
     3481 ctcgtaaatt caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt
     3541 ttgagattgc gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag
     3601 aaaaagagca atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt
     3661 tctgtcggac tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc
     3721 agtgtcattt tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa
     3781 caggaagtcg ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat
     3841 gaaacggact ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac
     3901 aggattattt ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt
     3961 ttatttgata tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt
     4021 agcaaatagt acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg
     4081 ggcatttttt gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt
     4141 aacagcaata ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg
     4201 aggtagcgca ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga
     4261 attggaatct tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg
     4321 tataggattg agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg
     4381 acagatcgat gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt
     4441 tttattcagt tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc
     4501 gcaaaagaat ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat
     4561 aggaacgatt ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag
     4621 tcctttagtg actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct
     4681 aatgattttg gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat
     4741 tttatatcaa acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa
     4801 aggcgtacct ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct
     4861 acattttttt gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag
     4921 ttgttttcta tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa
     4981 gaaacggaac actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat
     5041 tgctttcagc ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg
     5101 gtttgtattc ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca
     5161 gatacatttt gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa
     5221 aacttccctg cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac
     5281 agattgacgg tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa
     5341 gaagaacgac agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag
     5401 acaaaacgat ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc
     5461 attgcctctt ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc
     5521 gtagtgatcg tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg
     5581 aatggtttga agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa
     5641 aaaatccctg cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc
     5701 gttccagcag atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg
     5761 acgggagaat cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc
     5821 ggcgaccaga aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt
     5881 gtcgttgtag aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg
     5941 gtcgaacaac aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt
     6001 caaggcattc tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg
     6061 gattggcata tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga
     6121 ttgccagctg ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag
     6181 gcgatcatca aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca
     6241 gataagacag gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa
     6301 gaagcctgcg tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag
     6361 aaaacagaaa atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca
     6421 gccaatctac ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat
     6481 atggcaacga tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa
     6541 gtattatttg ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct
     6601 gcagaatttg cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg
     6661 aaacaggaac tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg
     6721 atcgatccgc caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc
     6781 tcagtgaaaa tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt
     6841 ggcttaaaac atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa
     6901 gagttgattc agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa
     6961 ctgcgaattg ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc
     7021 gtaaatgatg cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt
     7081 agtgaagtaa gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt
     7141 gcaaaagccg taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc
     7201 ttcttgccaa ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc
     7261 ccgctacctt tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta
     7321 tcttatgcac tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat
     7381 ccaaagcaag gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg
     7441 atcatggttc ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc
     7501 attcttctgc aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg
     7561 tccaagccgt cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt
     7621 atccttgctt tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt
     7681 acagtaagtt taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc
     7741 ttagttgtag aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca
     7801 gctagagaat acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa
     7861 acgattgagc tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga
     7921 atctagaggg aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc
     7981 ggttagttcg ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg
     8041 caagaacgcc ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg
     8101 tttgatagaa aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg
     8161 ttcattgatt tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa
     8221 gtgtgcatat tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc
     8281 aatcaatatc ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga
     8341 tgtttttttt agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag
     8401 gacctctaaa aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt
     8461 gtaattccaa ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata
     8521 aataccttcc gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct
     8581 gttggtatgg atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa
     8641 gaaccaacat gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt
     8701 tcttaaaaat tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt
     8761 atgagtgaaa ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag
     8821 aatacaagct gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt
     8881 gattttagtc acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc
     8941 agggacagca actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg
     9001 tttgaaaaaa atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat
     9061 tcctgcttga tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag
     9121 gtcagaaaaa atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat
     9181 ctttgtatga ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt
     9241 aatgattttt ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga
     9301 gaaaataaac ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc
     9361 atttactgat gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa
     9421 ataggtgggc aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc
     9481 ggattgaaca tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta
     9541 cttgtattca tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag
     9601 agatggaagc agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat
     9661 ttgtctccag aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa
     9721 agcaaaaaat ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca
     9781 agtgaccata ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt
     9841 ttgcgaggaa aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct
     9901 attgatctac gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt
     9961 aaatcaacgc aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct
    10021 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt
    10081 attttctaag aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat
    10141 tttgtcccag ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat
    10201 cattttgatc aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa
    10261 gggcaaatct ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac
    10321 ttttgggaaa aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc
    10381 atctgtcgtc gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt
    10441 atgacggaag gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa
    10501 ttgcagatgt tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc
    10561 tttcatgata ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg
    10621 atcggtaata tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt
    10681 ttccataaaa attcggacca agttcggaat agaaacagag gctacctcta aatacaatat
    10741 gacggaagta acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt
    10801 gtctaatgtc ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt
    10861 tctgccgcta tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg
    10921 gctcaaggca ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc
    10981 gaatactttt ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac
    11041 ctttttgtta tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga
    11101 tagcagtgga aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc
    11161 ctggacaagg cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac
    11221 gtttagaaaa tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg
    11281 tttttatcca gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa
    11341 acggtattca gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg
    11401 cttgtcgatc tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa
    11461 tgaaagaagc ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg
    11521 aagctgagga aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg
    11581 aaaattgtcc gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg
    11641 cagaagccaa acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc
    11701 actgtctctt gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc
    11761 tatctgatgc tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag
    11821 aagagattcg taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga
    11881 tggacttggc aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt
    11941 tgacaaagct gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag
    12001 accaacatgg tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg
    12061 aaaaaccaaa ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa
    12121 tcagcaactt gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa
    12181 aactgattgc tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt
    12241 ttctttcaga aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca
    12301 tgtctagtat ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga
    12361 tcgatttctc ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca
    12421 caatgaaatt aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt
    12481 tgattccaaa tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca
    12541 caggtttcaa tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta
    12601 tcgtgatcgt tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg
    12661 actgggtaga cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac
    12721 gagatcctca aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa
    12781 tctacgcaga acacggcgta caatctttga atcacggtat tggattcaat acagcagcta
    12841 tcgaattgtt actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga
    12901 actgggtatt gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa
    12961 gcatccatag ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg
    13021 tcttttttgt cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag
    13081 gacaatatgc catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact
    13141 cttctaccgt aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata
    13201 atcctggcgg tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc
    13261 cattagggaa aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa
    13321 aaccagtctt tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca
    13381 atgcaccagt gatgatttat agcgaagata ctacacatat cgtgacagaa gaaggaattg
    13441 cttatttgta caaagcaaaa acaaaagaag aacgacaagc agcaattgca gcaattggcg
    13501 gtgttacacc tcttggaatg actagcacga aagaatcact agatagcttg cgtgcagccg
    13561 gcatcgtgaa attaccagaa gatctaggca tcaaacgtag tgaagcgaaa cgttcattat
    13621 tagctgcaca aaccatcgat gatttgatcg aatggtcaga tggcctgtat gaaccaccaa
    13681 tgaaattcag aacttggtca taaaattggc gagaaaaacc ttttgtcgcc atttacccag
    13741 atggaagaag aaagacgaat aagcagaacg tgtatgctct gttttacaaa aaggggagag
    13801 atccatggaa caattagctt ataaatatcc aacgaaacag ccgatcaata agaaaatcca
    13861 tgtaggagtg gtcggttcag gagatttgga aatcttgatg tttccaacag aaaaaacaga
    13921 atctactgta aaaatttgta caggaagcga tggcttcggc gaagtatgga acaatgtatt
    13981 aacgagattt tttgatcgtt atccaatttc agcagatatc gtgatcaatg acttcggtgc
    14041 tacaccagga gttgtgtacc tacgattgac gcaagcaatg gaggaacttt cagatgaaaa
    14101 atagttttgt agaattaaac gccagagaac gagtgaacgc tctacttgac aatggacgtg
    14161 aattgattga tccattcgat caaatcatcg cgccaagttt accagcacaa ggaatcgtcc
    14221 ctgaaagtga tgacgggatc gttgtcagcc gtggcacgat caatggcaaa caagcagtag
    14281 taatcgctat ggaaggaaaa ttccaaggtg gcggtatcgg tgaagtttcc ggtgcaaaaa
    14341 tcgttgctgc acttgaacat gtgttagaag aaaataaaaa aggaaatgag atctttccag
    14401 tcattgtact agatacaggt ggtgttcgtt tgcaagaagc aaactacgga ctgttatcta
    14461 tttcagaaat cggaaatgta atcattgctc taaaacaata tgtaccagtc atcggtttga
    14521 ttccaggacg tgtaggatca tttggcggta tgtcgattac ttcagcatta atgagttatt
    14581 taatcgcaac aaaaaaagcg agagtcggtt tgaatggacc agaagttatt gaacaagaag
    14641 caggcgttcg agaatttgat tctagtgaca aagacttgat ttggaataca ttaggatctc
    14701 gtcaacgggt agaatcaaaa atcattgatg aattagtgac tgatagtgta gaagcaatca
    14761 aagctgctgt catcgcagca atggacgaac gaaaagacac gcatcgcagt gaaagctcag
    14821 cattttattt atcattactt gattctttag atctttcaaa accaatggcc attgacgatt
    14881 acaatgaagc aattgctaac catcaagcaa aatcaattga cttaccagaa gtagcagaag
    14941 gaaatgaacc agctgcagca agtgttggtt acgattggtt tgcggcacta actggcatga
    15001 aaaatccaac ttcttctatt agtactgtgt attatggtaa atcaagtaaa ttcatagaag
    15061 aagccatcgt gacatcaatc gtacctgatc ctaaaaaccc gatgtaccgt gtacgaaacg
    15121 gtgaagttgg tttagttgaa ggtttccgca tgggacaaat tttggatcac gtttacgaag
    15181 aagataaaga caaaaaagtg aaacggccaa tcatcgttgt gatcgatgta ccaagccaag
    15241 catacggcta taacgaagaa ttgatcggta tccatgtggc tttggctaat agtgctgctg
    15301 cttatgcaaa actccgtcaa gctggacatc cagttattgg attgattgca ggaaatgcta
    15361 tttcaggtgc atttttagca catggattac aatcaagccg cttgattgct ttaaacaata
    15421 aagaaattac cgtccaagcg atgtctaaag aaagtgctgc acgtgtgaca aaacgttcgg
    15481 ttgctgaaat cgaagcagct gctgaaaaag ttccagctat cgcttacgat atcaataatt
    15541 atacaaaact aggagcgatc tatcatttcc tagaagatat caccgaccaa gctgcttcag
    15601 aacaaaatgt cagcaaagtc atcgaagcaa tcaatgaagc cattcaagat gtccgcaata
    15661 caaatgacac gatgctgaca aatcgataca caaacgaaac agccaaaaca ttcggacgtg
    15721 tcgaaacaaa taaagtccgt gccaaaatgg acgaagaatg ggatgcataa aaaatgattc
    15781 aagcacatga tattgtcctt tttgataaac atgaaataga agggttcacc ttgcctgaat
    15841 gggtcgttct aactgaaaag atgttcggaa cagttcgtcg tgcgtcgacg gatcacgcag
    15901 aaatactacc gattggttta cgtggacctg cccgcaatca gagatggtca acagaaatct
    15961 caaaacatgc cgtcaaacaa gtcattcatc cgtgggacat catcgaacaa gaatcgtata
    16021 gacaagagaa aatcatggat tatcctgttt atcagcaatt tcatgctgct agaaacatat
    16081 tatcaggatg taaatgggga atcggcggca gcttaggatt tgaattatcg actgggattc
    16141 cggcagtaaa agaaaccagt gattttgatt tgctgctgta cgcaaattca ccgattgaat
    16201 tgccaattca ggtaattcaa tcgcatcctg ctttttttga gcaattcgat acccaagtag
    16261 tgactcggca aggcggtttt tcactaaaag agtatttgag agagccagag aaaaaaattc
    16321 tattgaaaac agtcgaagga cctaaattaa ccaaagaact ttggtaataa agaaaaagag
    16381 agagaaagag atgtttgaaa acaaaaaaat caaaatcaac gtaagtgact tccagaaagt
    16441 atcagctgta acggctgtta tgatgcaaac gatattgagc tttgctttgg caaacagcca
    16501 cagcgcaaca aatacagctt ttcttggtac cttttacgtc atcgttaaat acacagcacc
    16561 aatgtttatt tttgcaattg tttataatat ggtaaaaaca agtcagcatt tgtcttattg
    16621 ggaatttttg aaagaaaagt tttttgaact agtcgttccg tatcttctat ggacgacggc
    16681 ttatctttgg ctatttccag cagtccaaca acaaactccg tatacggata ctgcaagctt
    16741 tttactgaaa tatgtcacag gtgatggagc gcctcattta tggtataccg ttatgatgct
    16801 gcagatccaa ttgttcatgc cgttttttgt ttggctcggt tacaaagtat ttgctaataa
    16861 aaaatatgta tggccagtat tgattggttc aacgatttta tatgtcgcat ggtatctgtt
    16921 ctatcaaaca caagtattga ctgggcctta tcatgacagc tggtacttat tagatcggtt
    16981 tgttgctagt ttcatgattt atggggtagc cgcatttgtt tatcacgaaa aagtctatca
    17041 atatttggac agagtacgtt atcttttctt acctgttgcc ttagttatcg cgttcttttc
    17101 tgtccgttca ttgctcgcac atccaggaga cttgagcttt gcgaatgcac cttacttgaa
    17161 tacgatccaa agtttataca gtttagtcat catctttgct gtcttcatag gcgcttccaa
    17221 aatgattgta aacgattcgc caaaattgcc attgttcaaa tggttgtctg tttatgctta
    17281 tcgtacgtat ttagccaatg tatttgtttt ccaagtactg ttattattgt ttaaagatag
    17341 ttggctgcag ttaccttcag gagtcatgat tcttgtagct tatctaatga cggcaagttg
    17401 tgcatttgct ttaagctggt tacttcatat catttgggta gctatcaaaa aaggtttcag
    17461 taagtaatag taggaagaag gagtgagtgt catgataaat gacgaacgaa aaaaagcagt
    17521 gactcagcaa ctcgctgcgt tggtagagga agcattgatc gcagaagtaa cattaagtcc
    17581 aaaacctgga ttagtggatg cactggatgc gggtgcacat tccgatatgg actatgcttt
    17641 atttcttaag tctgctaaga cgctaactcc tttttttgaa gaaatggctc aaatagcatg
    17701 gcatcatgct attgatcagg agttaagaga aagaattgct gagattgggc gaaaagcgga
    17761 aaaggcaatg ctgattgcta caaatggcat caatacccat aaaggagcga tttgggcaat
    17821 ggggctattg atcagtgtca ttgcacaaaa aatcagtatg gaacagcctt tttctttatc
    17881 gaaaatactt gatgatgtca gcaagcttgc tgcttttcct gatacaaaat atcgtcataa
    17941 aaaaaccact catggtcata gggtgaagca aaaatatggc gtaaatggtg catatgaaga
    18001 agcgatatta gggtatccgc atatcaggct tgcaattcaa gcagctaagg agctagataa
    18061 tgattcatat gtacagaaac aaatgcatat gttgttagtt ttgatgagtt cgctagatga
    18121 cacatgcgta ttgcatcgca gtgatcagaa aacgttagtg gaaatgcaaa aacttgctca
    18181 tgctgctagt caaagtgctt taccaaatcg tccatttaca gcactgttgc acttttgtca
    18241 ggaaaaccaa atctctcctg gaggaagcgc agatctcttg tctgcaagct tgttcatgct
    18301 gaagatagag gaattatgga ctgccgacac atacatttgt ccgatgttac tcaatgacta
    18361 aataagacaa aattgctaaa aaaacagttt atgcatctgg ttattgactc ttccttttag
    18421 caaggctatc ataaaaatag ttttttgtaa taaaagataa cgaggaagac aatgaaaaaa
    18481 atcaattatt tagcaatcat tttgttagtg ggcctatgtt tgtttgctct gccgacccaa
    18541 ggattcgctg tcgcgggtgg tcatggtgga ggtacatcta gcggtcgaag ttcgggcaga
    18601 ggttcatttg gtggtcgttc atatgggggc agttattttg gcagtcgttc ttacggcaga
    18661 gattcttatg ggtattatgg tccaggtaca ggtccttcta gttatgagca aacgggattt
    18721 cttgcattag ctgttttgtc agtaggaggg gtttctgctt tttctatcgt caagaataaa
    18781 agacaaaaga aaaggctgaa agcggagata ttgtcccgca tgtcaggaac ggctaaagaa
    18841 aagaaagcac agtttctgga aatccaaaag atattcatga agatccaatc cgcttgggaa
    18901 gaagaaactc ctgaaaaagc cagaagctgt tatacagaca agctgtttgc cgaacatcag
    18961 aaagttctgc aaaaaaacaa agcagacggt gtaagaaatc atactaaaaa aataactatt
    19021 caggaagtag gaaattatcg acagatccac gaaaatagtt ttgccatccg aattgatttc
    19081 agatgctgtg attattcagt cgatcgaaaa gatgggcgat tgatctcagg aagtaagaca
    19141 caacgacagt atttttctca gatatggtat tttgattttt ctgagaaaga aaaaagatgg
    19201 caagccgatt ttattcagcc gattatgctg gattgacagg aaatatttat gaacggagtt
    19261 gtgacaaaag ccttgtcgca gctccttttt ctgaataaac ggtgtgccag agatagccta
    19321 tcggcaataa ggcacgattt tgtaaaatat gggaagcgat ttttcaaaat cgcttcttat
    19381 cgctcaaggc tgaacatctc tgtcacaacc tctccactaa atggtgttta aaagcttgca
    19441 tctgcgataa taagaacaac caaacgaaaa ataggaagca ctattttagt ttagttgttc
    19501 ttattacttg atgcaggaca gctttttcac aacctctttt ttgtatagat gggcaaaatt
    19561 tttgtaataa taggcgtatg tctttttcgt tgaggagatg aaaaagcata tggcttattt
    19621 tttgtttgct ttgcttactt tctttccact tcaagtggca gaaatatcta ctttttatta
    19681 tgtaagcgga accatatact tgaaatcaga aggagatcat ttctatgaaa acaagaacaa
    19741 ttgaaatact tcacagaatc atgacagccc aatatccagt aaaagcaaaa catctagcaa
    19801 gtgagttcaa tgtcagtcag cggacgatcc gtcaggaagt tcttgaagcg aatatctggt
    19861 tgagcagtaa aaagcttccg gagatccgta caattcgcaa caaaggtttt ttgttgaagc
    19921 tggaaaagga aaagcaagct cgattagaaa ctgaaataaa agatgtgaaa gacggctttt
    19981 tgaatcggcg cgaacgtatt tttgatctag ttttagccgt tgcttacagc cgaatcccga
    20041 tccatctaaa caaaaaagaa gaagaatttc aaatctctaa aagtacatta gatgaagata
    20101 tgagacggtt acgagcaaca ctggtcagtt atgggataga gatcgtcagt tatggaaagc
    20161 aaggactggt ttataaaggt tcagaacgat ctatccgtac gatgatctat gattttatca
    20221 atcagaatct gggaatgacc gacttcttta tgaatcaaga tgcacaatta acaccggctc
    20281 aaaaaatctt tctccattat tttcctttgg tggaaatcaa aaaaatacag gagatctatt
    20341 ccaataagtt gttgaaacaa gaagatgata tttataaaaa acaaatcctg ctttttacat
    20401 tgatctggat ccagcgaatc aggaaacatg aatttatctc tgccattaat tggaaaaata
    20461 cagaaataaa agacagtagc ttttctgcat ttattgacca catgatcgag gaatttcaat
    20521 tggacaatgt cccgcaagta gaaagaaatt atcttcgttt cactattgag acgttcaatg
    20581 ctagagatat ctccaattca atcgagtggg tacaagctca attactaacg atccaattga
    20641 tccaatttgt agagaacgag acacacgtac ctttccatct aaaggaagaa gctttattcg
    20701 aaaatctcta taagcacatg gctgcactga ttgtaagaat caagaatcat atccaagtca
    20761 tgaatccatt gaaagaaaac atccgaacaa attatttccc aatctaccgc gcagtcactc
    20821 attttgttcc tacgatcgaa gaagtaattg gtggcaagat actggaggac gagatagctt
    20881 ttcttgtcat ccacttttcg acgatcgcca gtgtaatcaa gcgagacttg aattatgttt
    20941 ataaatcagt cgttgtctgt aaccatggga tggctactgg aaatctgtta gcagagaatc
    21001 tgaaagaaaa gtttcctcag atcgagatta ctgcagtttt aagttcaaaa gaaatcgaat
    21061 tagtagataa attggatgtc aatctggttt tcagcacctt tcaattgaat tatcgaaata
    21121 agccgctttt agttgtcgat ccgattttaa ctgagacgaa tatcttggtt atagaagact
    21181 ttttgaaaag aaatgaagaa ctacagcggt tagagcctaa cagtcatgaa tcgacggagt
    21241 tattcactca aataatagat gtcatgaaag aaagtggtgg ccgagtagat cgaatcatct
    21301 atggcaagtt agaaaaatta ttcgaaacaa acaatttaga aatcaacaaa agggagatcc
    21361 agcctatgtt aaaagatatt ttgacggaca gcaatatcat gatcaaagaa aaagccacta
    21421 cttgggagga atcgattgaa ctagctgcta gaccattggt taaagaaaac attgtcgaag
    21481 agcgctatgt cgaagcgatg attcgtgcag tgaatgatta cggaccgtat atcgtgattg
    21541 gcaaacacat ggcactagcc catgcccgac cagaagatgg ggtcaataaa ttaggagtca
    21601 gcgtagctac gattgagcaa ccaatcgatt tcggtaatcc tgaaatggat ccagtaaaga
    21661 ttattttttg tttggcagca gtagactcct actcgcatct gaacatcatg aaagaactaa
    21721 ttgaattgat caacaacgaa gagaagttgg atcgattgat tgaatgcaca aatatcgaag
    21781 atttcaaaca actgcttttt tcagtagttg agaaataata aatgatacgg gggagaacaa
    21841 agatgaacaa attgaacatt ttattcgtat gcggtgcagg tttaggaagt agttttgcag
    21901 cacagatgtc tgctgaggat gttttgaaca aattaggagt agaagccaaa ctggaccatt
    21961 gtgatatttc ttccgcagtt tccatgaaac cagatgtgat catcacagca gaaaattttc
    22021 gttcgcagtt cgaaaagttc gcagttgatc ctaaaacggc gatgatctat ttgaaaaata
    22081 ttgtttcaaa aaatgaaatt gaagaaaaaa tcaccccagt tctgcaagat aagggagtta
    22141 tctaaataca aaaagaaaag agggtaaaaa
//
LOCUS       DAFGZE010000045        21936 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.046, whole genome shotgun sequence.
ACCESSION   DAFGZE010000045 DAFGZE010000000
VERSION     DAFGZE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.046"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(39..482)
                     /locus_tag="LTU54_001845"
     CDS             complement(39..482)
                     /locus_tag="LTU54_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296557.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(498..1658)
                     /locus_tag="LTU54_001846"
     CDS             complement(498..1658)
                     /locus_tag="LTU54_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296558.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            2165..3865
                     /locus_tag="LTU54_001847"
     CDS             2165..3865
                     /locus_tag="LTU54_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296559.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(3967..4800)
                     /locus_tag="LTU54_001848"
     CDS             complement(3967..4800)
                     /locus_tag="LTU54_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBL8296560.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(5104..7269)
                     /locus_tag="LTU54_001849"
     CDS             complement(5104..7269)
                     /locus_tag="LTU54_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8296561.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKNPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7529..9823
                     /locus_tag="LTU54_001850"
     CDS             7529..9823
                     /locus_tag="LTU54_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBL8296562.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            9816..10481
                     /gene="pgmB"
                     /locus_tag="LTU54_001851"
     CDS             9816..10481
                     /gene="pgmB"
                     /locus_tag="LTU54_001851"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBL8296563.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            10474..11481
                     /locus_tag="LTU54_001852"
     CDS             10474..11481
                     /locus_tag="LTU54_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HBL8296564.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            11503..12522
                     /locus_tag="LTU54_001853"
     CDS             11503..12522
                     /locus_tag="LTU54_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296565.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(12803..14170)
                     /gene="nhaC"
                     /locus_tag="LTU54_001854"
     CDS             complement(12803..14170)
                     /gene="nhaC"
                     /locus_tag="LTU54_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBL8296566.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(14279..15700)
                     /locus_tag="LTU54_001855"
     CDS             complement(14279..15700)
                     /locus_tag="LTU54_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8296567.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(15943..17820)
                     /gene="tdc"
                     /locus_tag="LTU54_001856"
     CDS             complement(15943..17820)
                     /gene="tdc"
                     /locus_tag="LTU54_001856"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HBL8296568.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRNVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(18111..19367)
                     /locus_tag="LTU54_001857"
     CDS             complement(18111..19367)
                     /locus_tag="LTU54_001857"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBL8296569.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(19479..19684)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            19947..21248
                     /locus_tag="LTU54_001858"
     CDS             19947..21248
                     /locus_tag="LTU54_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBL8296570.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(21317..21916)
                     /locus_tag="LTU54_001859"
     CDS             complement(21317..21916)
                     /locus_tag="LTU54_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8296571.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         6609 a         4588 c         3922 g         6817 t
ORIGIN      
        1 aaaattaggc tgggagcatt atttcgttat tctgttcatc aaaaatcagc gttttgattc
       61 catttcatca caagttcctc ctcatttact gcttcatctt tttgctgtgt ttgaattgta
      121 aaaccttgtg aaagatagaa atgaatagct gattgatttt tttgatagac tgtcaaagtc
      181 agtgttttat tggtttcttt tatttttttc attagttgcg taccgattct ttgttgccgg
      241 tattcccttt tcacaaagat tcctgcgata aactgatcaa tcaccccagc aaaaccaata
      301 atttcttgat ctatttctgc taagtaaatc actgcatctg gtaaagcttt cctaacagat
      361 tctacttgat tctcccaata agttgatgga atgaattcat gagaatccaa gttagcatgc
      421 agccaaatcg atagaacaga ttccagttct ttggcagaca attttgttgt tgtgcggatc
      481 attttttctc tccttactta ttcattgatt ttaaaataaa aaccattttt ttgaagctct
      541 tttacaagtt ttcgcccctc aaaaaagtat atcttctctt gttttacttt tctaatacca
      601 aaagaaacag caactttagg atcatcagct gaaacgattc gataagttcc accaggcaat
      661 tgatccagct tgactttccc atgatggtcg ctttgtactg aaaacggcac accatttcta
      721 gtaagtgttt ttttcttatg tttcaaaatg aatgaactac catttaccgg tttccctgac
      781 gaatcactaa taataaatga aaaatcaagc acttttttac ttaatctata ctgatagatt
      841 agtcgtccga taaacactaa cagtatgact acagctatta tcgttcctgc aagaataaac
      901 cattgccgca tggttctttc ctgatttcct tttccttctt ctttttttac cgattcgtta
      961 taaggaatcc gatgtcctgt cactagcaaa cgatgcgtgt tgatcatata cggcgtacat
     1021 gtaagcaagg taactaagtc ttctccttct gaaatcgtta gaacagacgt gtcatctggc
     1081 agtaccactt gtatattttt aacttgataa gccaatttct cccccaaaac agtcaaaata
     1141 aacgtgtctc cgttttctaa acgatctaat tcggtaaata accgtcgatt aggtaaaccg
     1201 ctgtgagcag agatcactga atgcgtcgaa tttccaccaa gcgggaaaga ggttccttgc
     1261 aagacgcctg ctccattttc aaggatccta ccattgagcc gatcataaag tgaaatattt
     1321 aattggatct taggtatatt gatacttccg atcagccatt cctctaattg gctggatgtt
     1381 gtactttcat ttctgttctt ttcattgaaa ggatcagtac caggattgaa tccttggtca
     1441 gttaatcgtt ggtttttctc ctccattttc tttcgctgca cttcaatttc agctgcggtc
     1501 cgtttttgta cttgttcgat acgtttttga tcaataaaac tatttaaaga atccacataa
     1561 aatggatata aagcgatcag taaccctacg acgatacata ttaataaaaa caaacgaaac
     1621 aaaagtcgac gagactgttt ttgcttactc tgcttcacat gtgtcctccc ttgcaagttt
     1681 ttttgttctt tcttttatta tatgggttca ggaaaaaaat agaaaatgaa agccgatgag
     1741 gttgtgaaaa agctgtcctg catcaagtaa taagtacagc ccaaaaaaat agctactcat
     1801 attttttgtt gggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
     1861 gaggttgtga aaactgcgtt atgctttgag taataagagc gctagtcaga aaatagcttc
     1921 tcatattttc taggctagtg agcttattac cgagaagcag ctgattgaac accgtttacc
     1981 gagaggtcat gaaaaagcta ctttgtttcg agtattaaga aaaattctgt ttcaaaacaa
     2041 ggagatgcaa caaaacttat gtcgcatcct ctctccttag aactttcact tatgtttttc
     2101 ttgtaaaatt cataaatatt ttcatgaatt ttctaatttc ttgcctacct ctcgcatagg
     2161 atatatgatg aatttatcta aagtttgcaa aggagttcga caattgaaaa aatatggccc
     2221 atttttagca atttttttca ttgctttggc atttctcatg cctcatttat tcacacataa
     2281 actaattttg ggtgctgatg ccccattcca ttacaatcgg ttctatgatg ccgcgcaaca
     2341 aattaaatat ggggatttcc attattttcc ttccctttat ggattccagc aaagtggcag
     2401 gatcgtgaat gccgtttacg gcccattttt cgcttattta caaggattga tcgtgttgct
     2461 tgcaggtagc tggtttcatt atcagattct ttcaaatttc attctttatc ttatttcagg
     2521 attttctatg ctgattttac tgagatatgt caatgttcga aattgggtaa gtgtaccagt
     2581 agccgtttta ttcatgagta cgtattctat tcaatactgg tctatcaacc aaggattcac
     2641 tagctggggg acttgttttt atcctttatg tatgatccct attgctgatt tcgtccttca
     2701 taaaaagttc cctgttatca aagtcgctgt ctcagtggga cttatgacac agatccattt
     2761 actgtcttcg atcatgttag ctttgatgta tatccctttt tatctttatt acttttttaa
     2821 ccagactgaa aaaggaaaag cactgcttca actgattaag tcggtcttgc tttattttgt
     2881 tctgacagca aatatttggg caggattagc tattgtttac agtgggaata agattcttcc
     2941 tccctttgtt aacagagaaa tgagtcaaaa ggcgatagat ctggatggca gttattggct
     3001 ccattaccct aaggcctttc ctatattgat attatcaggt tttttgcttt ttattttctt
     3061 tcaacaaaag aacaccttat tccaaaaaat cgtgttgtac acaagtcttg ttttcttttt
     3121 tctttctact agcttgattc catggacgac attgatcgaa aaaaatgtgc cttttattag
     3181 tttgatccag tttccatttc gatttttcgt gccatttacg ctattgcttc tactttacct
     3241 tgccctttca ctgaaccagt ggtctgaaca aaagtggttc cgtcttttaa gtatatttgg
     3301 cattcttatt ttttcagcac agacgatcca gaacttatac cagcatctgg aaaagtggga
     3361 aaatgaaaca tttgtcagtc gacatactta tctattcgat acgccggaag aagcaaggaa
     3421 aacattcttc agcaaagata tgtctaccag cttgttcgtt ttccaaaaaa ccacacctga
     3481 ctatctgcca atctataagg agacaaaaga caataaatac gaccggtata acgaagaaat
     3541 actagaaaac gaaacaactt ttataaaaac acatccaggc ggtcttttga caatcaagtg
     3601 gaaaaacact ggggacaaaa ctgttcatat ccctgttatc gtctacgatc ggactgtgct
     3661 acagcaaaat ggaaaaacat tgactgatta tgaagtaaca gatatcggga ccccaatcgt
     3721 gaagcagcaa aaaggaatca acgagctcac gcttcattat caaacgccaa tctatttcta
     3781 tttcattctt tctcttactt tgattggctg gtttaccttg ctatgtttat ttatttatca
     3841 tagatacaag cttttacgtg cttaggcaaa agggataaat acatccgatc aaaaaactct
     3901 ccaaacggta taatcgtttg gagagtttta ttttaatcag tttttttatg ttatatgtta
     3961 tattttttat tcgttcacat ctaattcgat ttccaccccg tagtgatcac tgatgattgg
     4021 actatcgatc ccatcaaaaa tccgttgata ttttttaacc tgcattcctt cagggacgaa
     4081 aataaaatct atacgcaatt tttctgtatt ttgttcccaa ccatctattt tcttttccac
     4141 agtagctgct tttccttttt cattcgcgac aacatacgaa tcttgaagaa gaaggcaact
     4201 ttctcttaca gtataataac cttgagagtc tactggatta ttgaaatcac ctaggaaaaa
     4261 aagtggtacg cgagtttcca aaaagtagtt ttccaagttt ttccactcaa aataaaagcc
     4321 acttttttta tctgtccacc aagaaaagtg gcagctcgag acaaaaatgt cctgatcatc
     4381 tatctttgtt ttcccaatca agatcttgcg tgtctgataa tcttctttct tttgaaattg
     4441 ggagacggta aggacctgac tttccaatgt ttttttcgta agcagtgcat ttccttcttc
     4501 atatttatca taaccgatgt ggctcatttc ccaactccaa aaatactctt ttccgtgttc
     4561 ctttaaaagc tgaactaaac ggtaggcaaa attatcttta cggataggta cttgattctt
     4621 taccggacag aacttcatta gcttcgcagg ttctacttct tcagattcca gtaattggtt
     4681 gatttcttgt aaagcaatca cttcataatc ttctaacagg atcttatctg ctaattcttg
     4741 caattttttc tctggttctg gctcaagcca gctatgcgtg ttgagtgtca tgaatttcat
     4801 tggttttctc cttgtcttca tttcgaaaca aaagggttgt gacaaaattt ttgtcacaac
     4861 ctctccagca aatagtgttc tcctgctaac tcctcagtaa taagctcaat ggaccaggag
     4921 ctattttttg tttggctgtt tttatcactt ggtgcagggc agctttttca cgacctctcc
     4981 actaaacagt attcaaactg ctgcttctcg gtaataagcc caaccaaacg aaaaatagga
     5041 ggagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt tcacaacctc
     5101 tccttacatt ttaatggagc cgatcacttt accagctgtc actgtaccta attgcttgat
     5161 cactacttct tctactttat cgtttgtaaa tacgatcatg atcgttggat ctttttcttc
     5221 gttttggatc acatcaagat ccatctcagc taatttcgtt cctgttccta cttcttggcc
     5281 ttctttgaca gcaatctcaa aagctggctg attcatttgg actgtatcga ttcccatatg
     5341 gatcaaaact tcgataccat ctttcgtttc cagcccaatt gcatgtttgc tagggaaaat
     5401 acttgtcact tttccagcga tcggcgcaac aacttctctt gatgttggaa taacagcaaa
     5461 gccgtcaccc atcatttttt gtgagaatac cggatttttt acttcagata aaggaatcac
     5521 ttctccagaa gctgcggaaa ctagttcttg ttctatacct aagtattgct tactacctgc
     5581 ttgtccattt cctgtagaaa ccatttctgg ctctggaatc tctgctcctg attgcagcag
     5641 atcttcgata tctgatttca agacatctgc ttttggtcca taaactgctt ggactccatt
     5701 gtctttgacg atcaatccca tcgcccctgc tcgtttccag gcttcttcag tccctacttt
     5761 ttctcgatca tttacactta cgcgtaaccg agtcatacaa gcatcgacat ctttgatatt
     5821 ttcttttcct cccagcaaat gaatgatctg gaccacttgc tgatctgctg ttcctgttcc
     5881 gctgttagat acaacggttt cttcactgtc attgtcgtaa tttccgttac gtcccggtgt
     5941 agcaaagttg aattttttga tcaagaaatt tgctaagaaa tacgtaacaa caccaaaagc
     6001 gatcacacac aacacgaagt tgataagatc tccacctaga cccgctttga tagctaacgg
     6061 tgtccgagtc agcaattcga tattcccaaa tgaatggacc cgcaatggca agatatctgc
     6121 catcgcaaat gcagcccctt ggatcactga ataaatgaca tataatggca cagcagcaaa
     6181 cataaacatg aactccaatg gttctgttac acctgttaga aagacagcca acgctgctga
     6241 aaaatacatt gatttatatc ttgctttctt atcaggatcg acattacgat acatcgcaaa
     6301 agccattccc atcaagatac ctgaagaacc aatcatttgt cctactttga aacgagccgg
     6361 cgtccagtta tgcaacacaa actcatattg cgacatatcc cctgcacctt taagattcac
     6421 taaatcagtg gcccaagcca accataacgg atcttgccca aatacttgtg tccccgcctg
     6481 tgcgcctgat aaaatctcat acgatccgcc aagctgtgta tagttgatag gaatcgtcag
     6541 catatgatgc aatccaaatg gcaatagtaa acgttctaat gttccaaata aaaacggtgc
     6601 tagaatcggt gcgctttctt gtgattctgc aatccataaa ccaaaattat tgattccagc
     6661 ctgtacgttt ggccacacca cagcaagaat caatgccacg attgttgacc ataagataac
     6721 aacaaacgga acaaagcgct ttccattgaa aaatgacaaa gcatccggca attttctata
     6781 attatagtac ttattataag ccattgcccc gacaaatcca gcgatgatcc ctacgaatac
     6841 tcccatattc aaagctggtg cttccaatac acttgtaaaa aatcctttaa ccatgatctt
     6901 cgtaccgaac agagtgtggg taaatgcatc ttcattcgca agcatgtcac ttgtgacacc
     6961 aaagatagcc ccagtgatcc gattgattaa aataaatgag atcccagctg caaatgctcc
     7021 gcctgctctt tcttttgccc aacttcctcc tattgctaaa gcaaataaaa tatgaagatt
     7081 accaataatt gcccaaccaa tattttcaat tactccgccc gttgtgacaa gcggcgtcca
     7141 atcaggattg atcaagggaa ttgatttccc tatactgatc atcaacccag ctgccggcat
     7201 aactgctacc acaaccatta aagcctttcc aaatttttgc caaaactcaa aactaaatat
     7261 ctttttcatt ccgtttagct cctttacgca atcggtttcg ttaaacgtat tatagcgtgc
     7321 gttttctttt ttgtaaatgc tttatttttt atttttctgt ctataccatt ctataaaacc
     7381 tataatacgt gtgttccctt ctataatttt ctcctaaaaa tttatttttt taagcttttt
     7441 tctatgaaat attttctttt gtctattgac ttgtgcaatc ggttgcgttt attatgaacg
     7501 tataaaacag ctcattagga ggagaaaaat gaaacagatc aaacgactat ttgctattga
     7561 tccatggaaa atcagcacga cacaattaga taaaaaaaat cttcgactgc aagaatcttt
     7621 aacaagtatt ggaaacggtt atatggggat gcgcgggaac tttgaagaaa cttactcagg
     7681 ggaccaccat caaggtactt atttggcggg tgtctggtat cccgataaga cacgtgtcgg
     7741 ctggtggaaa aatggttatc cagaatactt tggaaaagtg atcaacgcct tgaactttat
     7801 cgcgatagat atttcgatca acgatcagct gatcgacttg gcttctttgg aaccagaaga
     7861 cttttactgg gaattagata tgaaaaatgg tgtattatct cgtacattca caatcactac
     7921 ttctactaat aaagtacgca tttccttcga acgttttctg agcattgtca aaaaagaatc
     7981 agcttatatc tatttaacta tagaaatgct tgaaggtagc ggaacagtca aactcgtatc
     8041 aaaattggat ggaaatgttc aaaacgaaga cagcaactat gatgaacatt tttggaatga
     8101 gattgatcgt ggaactatag aagaaatcgg ctatttgacg actaaaacta ttccaaacaa
     8161 ctttgggatt gaacaattta ctgttactgc agcaatgcgc catttcgtta atggaaaatc
     8221 agtcatccct gactatcacg aagatacttt agcattacat ggttgcttgt ctgcagaatt
     8281 atctcaaggt gagacatggt cagtggaaaa agaaattatc gttcttacta gtagagatat
     8341 tccagaagcg cagcaagcag aaaaagctgt ctcagaatta acgaaccaga caaacaatta
     8401 tatccaagca aaagaagagc aaacagcggc ttgggcagaa cgatggaagc ttgccgatgt
     8461 ggtcatcgaa ggagacgaag aagcacaaca aggtattcga ttcaatttgt tccaactatt
     8521 ctctacttac tacggcgaag atgaacgatt gaatatcggc cccaaaggat tcacaggaga
     8581 aaaatacggc ggagccactt actgggatac agaagcgtat gctgtccctc tttatcttgc
     8641 tttagccaaa cctgaagtaa ctaagaatct gctgaaatat cggcataatc aactgccgca
     8701 agcaatccac aacgctcagc aacaaggatt aaaaggcgcg ctttatccta tggtcacctt
     8761 cactggtgtc gaatgccata acgagtggga aatcacattc gaggagattc accgaaatgg
     8821 cgcgattgcc tatgcaatct acaattatac gaattacacc ggcgatacgg aatatttgaa
     8881 aaatgaagga ctggaagttt tagcagaaat cgcacgcttc tgggcagacc gtgtccattt
     8941 ttcaaaacga gcaaataaat acatgatcca cggtgtgacc ggcccaaacg aatatgagaa
     9001 caatatcaat aacaattggt atacaaatac gatcgcagct tgggtcttgc gttacacgcg
     9061 agaaaattat ttaacttatc aaaaagaagc tgctatcact atcagcgaag aagaactagc
     9121 caaatggaaa gatatcgcag agaacatgta cttcccagtt gacgaagaat taggaatctt
     9181 tgtccagcat gacacattct tagataaaga tctcatgcct gtatccgata ttcccacaag
     9241 cgatcttcct ctgaatcagc attggtcttg ggacaaaatc cttagatctt gcttcatcaa
     9301 gcaggcagat gtactacagg ggatttattt cttcaatgat acttttacaa aagaagaaaa
     9361 gcaaaaaaac tttgatttct atgaaccaat gactgtccat gagtcttccc tttcgccaag
     9421 tatccattcg attttagcag cagaattagg aatggaaagc aaagctgtag aaatgtacca
     9481 gcgaacagct cgcttggatt tggataatta caataacgac acagaagacg gcttgcacat
     9541 cacttccatg actggtagct ggttgtcgat tgtccaagga tttgctcaaa tgaaaactgc
     9601 ccatgatcag ttgagattcg caccatttct gccaaaaact tggaaagctt attctttcca
     9661 tatcaattat cgcggacgtt tattgttcat ctcagtcaca gatgaacaag ttactttcga
     9721 gttattagaa ggttctcctc tagaaattta cgtctatgaa cagtcttatt ctttagcgga
     9781 tcgtctagaa ataacgctaa gaaaggagga agcaaatgtt taaaggggta ttgtttgatt
     9841 tagacggtgt cattaccgat actgctgaat tccattatcg tgcttggcga aagcttggtc
     9901 aggaaatcgg cattacgatc gaccacacat tcaacgaaca gctaaaaggc gtcagccgag
     9961 aagactcact ctctttgctt ttagcttatg gcggaaaaga acactctttt tcaaaggaag
    10021 aatttgcaga attagcgaaa agaaaaaacg attattattt agagatgatc cagacgatcg
    10081 aaccaaaaga tgtcttcccc ggggtcgtgc ctttactgga ttctttaaaa gaagaaaaaa
    10141 tcaagattgc tcttgcttct gcaagtaaaa acgggccatt cctacttgaa aaaatgggtc
    10201 ttacacctta ctttgatgct attgccgacc cggcagaagc aacaaacgga aaacctgcgc
    10261 cagatatctt cctccttgca gcaaaagctg tcggactaaa agcagaagaa tgtatcggga
    10321 tcgaagacgc gcaagccggt atccaagcca tcctctcaag tggcgctcaa ccaattggcg
    10381 tgggacgtgc tgaagacctt ggagaggaga tcccgcttgt ccctgatacg acttttctta
    10441 cgatcgatta cttgaaagaa aagtggcatg accatggata atccagagat ctccgtcgta
    10501 ccagttccag agactgaaat aaagaaaatc acattgaaga acgactactt agaagtcgtt
    10561 cttctcaatt acggtgcccg cctgcaccaa atcttcgcac cagacaaaga aggaaaatca
    10621 gaaaatgttt tattgtctta cgatcgtttc gaagatgttc taacagacaa aagctttttt
    10681 ggagcaactg tcggacctgt agccggaagg atccgggatg cttcttgggg tgagcataga
    10741 ttggagaaaa actgcggatc acaccatatc cacggtggca cgaaaggctg gtctttccaa
    10801 ttttgggaag tagaagtttt caaaactcca tcatcaatag gtgtggtctt ttacttaaaa
    10861 gatgaatttt ctacctatcc aggaccaatc actgctacga tcacttaccg tttaacaaaa
    10921 aacgaattag agatgacaac ctctgctagt agccttgttg aaaccatctg caatccgacc
    10981 aaccatgcct acttcaacct ttcaggaaat ggaacaagag acatctatga acatcagtta
    11041 acggtctttt tagatggtgt gcttgaatta gatcaagaaa aactgcctac tggaaattgg
    11101 acaaaaaaag aaaaactgcc aatcgacttt agaaaatccc caacactgca agagatcctt
    11161 gcatgttatc cagatggact agatgatgta tttcttttgc atcatcctag gctttccaaa
    11221 acttccttac aattattcga gaagcattct ggtcgccaaa tgactatagc aacttctaat
    11281 aaaagtatgg tattattttc tacgactgga ttcgaagctg atttctctgt caatggaaaa
    11341 cagatgcatt caaactacgg attagcaatc gaaccgcaag aaatccctga tatcgttcat
    11401 ttccccaaat ttggatcgat caacctccat cctggtcaag aacggatcag tcagacgatt
    11461 tatcggttct ctgcacaata acaagagaaa ggtgttttta tcatggctat taccgtaaaa
    11521 gatgtggcga aaaaagccgg tgttgccacc tcgaccgtct ctcgtgtcat caacgatcat
    11581 cctagtattt ccgagacaac aaagaagaaa gtccgaaaaa ttatggatga actaggttat
    11641 gttcctaaca tgactgcccg caatctagga aaacgcattt ccagtgccat cggcgtaatc
    11701 ttaccccctc ttgattcaaa agagcggatc ggtaacccat tttatcttga gatcatggaa
    11761 gcaatcaacg aagaagcgcg gaattatgat atgactacag caatcgctac tgctaaaaat
    11821 tttgaattgc tgttagagaa cgtccagcgt atgcatttac aaaaacaagt agatggtttt
    11881 attctagtgt attcagatag tcaagatact gtcattgatt atttatacca aaaccacatc
    11941 cctttcactt tgatcggaca gccttatcat catgagaacg aaatcgttta tgtagataat
    12001 gataaccagc ttctaggtaa acaagcaaca gaatttttga tccagaatgg acataagaat
    12061 atcctgtttg tgacaaacac gactcatgaa agtttgtatt tcgaacgata tttcggttat
    12121 caaaaagcaa tgatgatggc tggtcttcct gcgttatcca gtgtagtact agagcatccg
    12181 gaggattatc tgtcctttga gaaaacgttg aaagaaaacg aagccacagc attggtggtc
    12241 attgacgata tcttcgccct tcgcacgatg cagttggcgc aaatgtatgg ctatgtcatt
    12301 ccagagacac tctccgtcat tagcttcaac aactcgatct tctcaacatt aacacaccct
    12361 taccttacaa gtatcgatat cgatatctcc gaattaggac gtgtcgctat gcaaaagctg
    12421 cgcgaattga tccatgaaga attgagcagt ggcgtacgtt tggtcatccc tcatcgcttg
    12481 atcaaaagag agacagtcgt tcctttagaa aacccatact aaatagctga ctcactaaat
    12541 acgctgacca tttatcctat ggtcagcttt ttatgtaaaa gaagatacta cttctaacac
    12601 attgtttatc tgtaaaaaag agcgtgggac aaaagtaaag aacacttttg tctcacgctc
    12661 taaacacgta taaacggcgg aagcagaagc aactccttcg gaaataagct gaaattcaca
    12721 aaaatttaaa aagcaatttt cttgagtttc ttcttatttc ttggagttaa acgcttctgt
    12781 cccagcctct tttttccttc cttcaatttt ttacacgtcg acgattcaat aaaaaaccgc
    12841 ctaatattgt aaagacaggt gcaagaatcg agaaaaaggc gaatggcaag taatgcaacg
    12901 cgctaacttt catcgttccc ataatgaatg tgccgctgac tccccacggt acgagagaat
    12961 tgacggtcgc acctgaatcc gctagtgttc gtgtcaaata ggtttttgat aagccaacgt
    13021 catcaaaaga acgcttgaat gtttctcctg gaagaatgat ggacagatac tgttctccaa
    13081 cgatcagatt gattccgata cagctcaagg cagtaaaccc gactaaacga gaaggtcggt
    13141 ccatcttttc ttttaattct tgaacgatgg tatcaacgat cagatatttg atcagtaacc
    13201 cacctaatgc caatgcaagt aaaatcaaac tagcagaacc taacatactc atgatcccgc
    13261 ctcgagaaag cagttcatct agctttggat caggtgtatt tgcgacataa ccattcatca
    13321 agatgtctgc tgcttttccg accgtcaaac ttttctcatg gataaaagaa agaacgagag
    13381 cagctgtcga tcccgctagt aatgtaggga tagccggaat ttttttgatt gcccccccaa
    13441 ataaaataac tagtggaatc aatgtccaag gagagatcca aaagctcgct ttcatgtttt
    13501 ggatcatttc attgatacta gctagatcgg ctgaatgact gttatgtcct aatatcgtat
    13561 aaaaaatcaa cgaaaggacg aatgctggga aatccgtata acgcatacca gcgatatgtt
    13621 cgaatagatt aactcctcca atacccgctg ccaagttggt cgtatctgat aaaggcgagc
    13681 agttattccc taaaaaggca ccggaaacga ctgcgcctgt agtcattgca tcattgaatc
    13741 caagtaaatg accgatcccc aaaaaagcaa ttccaatcgt cgaaatcgtt gtaaatgaac
    13801 tgccgacagt cactccgaca attgcacaaa caagaaatgc tgtcggtaaa aagaaacgca
    13861 cagaaacaag tttaaatcca taaaccataa tcgtagggat ggttccagat aaaatccatg
    13921 aagcaactaa gacaccaatc aaaagaaaaa tcaggattgg aatgatccct ggagtgattc
    13981 ctttgatgat cccgtcatgg atctcatccc atgtgaactt tttaaatctg ccataaaaca
    14041 acaaacacat gaaagctgca aggatcggga tatgtggcga gagctggaat ccgatgatca
    14101 gtactcctag gagtgccaac aacacgatca atacagcgat actttctttt aaagaaatat
    14161 ttttattcat cgtcgatctc ctatttcaaa tgaaaaggcc ctgccatgaa aaaactattc
    14221 acttggcagg acctcctact aaatcagcga caaaacgtcg gctgaaagac ttattttttt
    14281 atttttcttc taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga
    14341 tgatcaatgg aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca
    14401 atgtcgctgt acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc
    14461 tgactaccca agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg
    14521 gatgtttctt acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa
    14581 taacagttgt agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat
    14641 aaagcaaagc aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc
    14701 cgtatttgtt ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt
    14761 ggatgattgt ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa
    14821 ataccatgaa gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa
    14881 tcaagatagg ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta
    14941 caagcatcgc attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt
    15001 ttgtataatt ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc
    15061 caacaaaaat aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag
    15121 ggaaagcttt tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga
    15181 aggctgccaa aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata
    15241 caccgatgtt cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc
    15301 agtagataac taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac
    15361 caaatgtgtt tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca
    15421 tttgcaccca taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta
    15481 cccataactg aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg
    15541 atatcggacc ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga
    15601 ctgcggctaa cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa
    15661 aggtcccaag acccatttta cttacttcag atttactcac cgttgacact ctccttgatt
    15721 ttttggataa aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg
    15781 aatatttgag gggattcttt gagtttattg cttgaagggt ttcttcaaag acaacctcaa
    15841 aacaaaggga tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga
    15901 aacagttagt attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc
    15961 caataattgg tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc
    16021 agcatattct tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa
    16081 tacagttact tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa
    16141 ttgtaatggg ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc
    16201 attaccgtaa atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt
    16261 attcattgca actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa
    16321 atctggataa gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc
    16381 attcaagaag ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata
    16441 tcctgtgaca ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc
    16501 tgcttttgaa ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc
    16561 aaatacatat gtcgcaaagt aagagataac gtttctcatg cggatatctt tgatgacgat
    16621 accaccggca gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc
    16681 ttcttcgatt gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt
    16741 aaagacattg tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc
    16801 ttcgtctaag aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa
    16861 gtaaataccg tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat
    16921 tgctccttct tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc
    16981 tgctagacca cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac
    17041 tggcactgga atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga
    17101 atagtgttta gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg
    17161 tgctgagtga gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat
    17221 ttcttctgtt gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt
    17281 cacttctttc attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa
    17341 gtttgctaaa gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt
    17401 tgcaaattcc attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc
    17461 aacgttatta ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt
    17521 ttcagagttc atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg
    17581 catccgcgct gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact
    17641 agcactgctt ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg
    17701 ccagcctaaa tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc
    17761 agctttgtcc ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat
    17821 agtgaaaccc tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt
    17881 ctattctgta aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat
    17941 ccgaattgtt cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact
    18001 ccctcgcaaa gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt
    18061 ggaatgttgt aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt
    18121 actttggcca aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat
    18181 tcatcggatg gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata
    18241 gctccgtttg aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca
    18301 agcagttcca cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat
    18361 ccttgcgcga tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt
    18421 attttttgcg cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc
    18481 aaacggcgtt gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg
    18541 atttcttctt gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct
    18601 tgattcaacc agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg
    18661 gcaccgcctg cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt
    18721 aaaccgaacg cttctgcttc tggtccttct aattttcgga ttaggtctaa tccagctgtg
    18781 atatttcccc attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga
    18841 tgcaaaaagt cgatagattg caggatttgg taagtaaact ctgtaaatga gattcctact
    18901 tccaaacggc tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg
    18961 tagtctctca aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat
    19021 gtgatatttg catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta
    19081 tgttgcactt gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg
    19141 atcgttcctg ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt
    19201 ttcatcatca taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg
    19261 ccgcaataaa gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt
    19321 tgctgattaa tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc
    19381 gtgtagtttt tttatgaaag caggaacctg ctagcatatg taattaatta tttcttcagg
    19441 ataggatagg aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt
    19501 accacccaat ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg
    19561 tggcaatcac tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc
    19621 agcgaccacc agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct
    19681 tgttattatt aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg
    19741 atattttttt taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt
    19801 attttcaatt attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga
    19861 agtcacttta ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa
    19921 aaatgaaaaa gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca
    19981 cttctgcttt aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta
    20041 caaaaaaaga accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag
    20101 atgataagca acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc
    20161 aagcgattgc gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc
    20221 ttcaaacact aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct
    20281 ctcatgtctt tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg
    20341 cacaaggata caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac
    20401 tttatcaaaa tgattttgtt ttagttaatt tgaatgattt gatccaaaaa gacgaacaag
    20461 gaaatctttc ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac
    20521 aagatgatgt cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg
    20581 tatcaaataa caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg
    20641 actttgacat ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc
    20701 gaggcgcaaa aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat
    20761 caaaatctca atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag
    20821 tggcggaaca gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga
    20881 atatgactca agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag
    20941 ttgcacctat cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg
    21001 actggcagcc ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg
    21061 atattttctg taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct
    21121 atcgaaatgc tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa
    21181 atcctattct tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat
    21241 ctgcttgatt ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt
    21301 tcggcttttt tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc
    21361 attctgctgt atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca
    21421 ttgcttgaaa atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt
    21481 tccgccctcg atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca
    21541 tcgcgtcaga aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa
    21601 attgtttgtt cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt
    21661 cttcaaatgg aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa
    21721 atgcaaaaaa tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg
    21781 ctttacttac ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt
    21841 ctgtgttcat caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct
    21901 tttcaaaacc gttcatttgt agagcctccc gtaatt
//
LOCUS       DAFGZE010000046        21839 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.047, whole genome shotgun sequence.
ACCESSION   DAFGZE010000046 DAFGZE010000000
VERSION     DAFGZE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.047"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LTU54_001860"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="LTU54_001860"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBL8296572.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LTU54_001861"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="LTU54_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBL8296573.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LTU54_001862"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="LTU54_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBL8296574.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LTU54_001863"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="LTU54_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBL8296575.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LTU54_001864"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="LTU54_001864"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBL8296576.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LTU54_001865"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="LTU54_001865"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBL8296577.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="LTU54_001866"
     CDS             complement(8053..8523)
                     /locus_tag="LTU54_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HBL8296578.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="LTU54_001867"
     CDS             complement(8569..8994)
                     /locus_tag="LTU54_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBL8296579.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="LTU54_001868"
     CDS             complement(9061..9330)
                     /locus_tag="LTU54_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBL8296580.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="LTU54_001869"
     CDS             complement(9341..10936)
                     /locus_tag="LTU54_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8296581.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LTU54_001870"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="LTU54_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBL8296582.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="LTU54_001871"
     CDS             complement(14489..15049)
                     /locus_tag="LTU54_001871"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBL8296583.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="LTU54_001872"
     CDS             15402..16376
                     /locus_tag="LTU54_001872"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBL8296584.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="LTU54_001873"
     CDS             16546..17946
                     /locus_tag="LTU54_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBL8296585.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="LTU54_001874"
     CDS             complement(18024..18746)
                     /locus_tag="LTU54_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296586.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="LTU54_001875"
     CDS             complement(18825..19331)
                     /locus_tag="LTU54_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL8296587.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="LTU54_001876"
     CDS             19454..21253
                     /locus_tag="LTU54_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBL8296588.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21839)
                     /locus_tag="LTU54_001877"
     CDS             complement(21321..>21839)
                     /locus_tag="LTU54_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBL8296589.1"
                     /translation="SSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPK
                     PVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIG
                     GWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGS
                     VQWFAPDFAVSM"
BASE COUNT         6051 a         4789 c         3966 g         7033 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaaga
//
LOCUS       DAFGZE010000047        21823 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.048, whole genome shotgun sequence.
ACCESSION   DAFGZE010000047 DAFGZE010000000
VERSION     DAFGZE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.048"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..108
                     /locus_tag="LTU54_001878"
     CDS             <1..108
                     /locus_tag="LTU54_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8296590.1"
                     /translation="YIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            236..1450
                     /locus_tag="LTU54_001879"
     CDS             236..1450
                     /locus_tag="LTU54_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL8296591.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            2439..2648
                     /locus_tag="LTU54_001880"
     CDS             2439..2648
                     /locus_tag="LTU54_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HBL8296592.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            2760..4373
                     /locus_tag="LTU54_001881"
     CDS             2760..4373
                     /locus_tag="LTU54_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8296593.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            4408..5619
                     /locus_tag="LTU54_001882"
                     /pseudo
     CDS             4408..5619
                     /locus_tag="LTU54_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            5642..6568
                     /gene="glsA"
                     /locus_tag="LTU54_001883"
     CDS             5642..6568
                     /gene="glsA"
                     /locus_tag="LTU54_001883"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBL8296594.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6776..8236
                     /locus_tag="LTU54_001884"
     CDS             6776..8236
                     /locus_tag="LTU54_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296595.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            8457..10220
                     /locus_tag="LTU54_001885"
     CDS             8457..10220
                     /locus_tag="LTU54_001885"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBL8296596.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            10304..10762
                     /locus_tag="LTU54_001886"
     CDS             10304..10762
                     /locus_tag="LTU54_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBL8296597.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10871..11554
                     /locus_tag="LTU54_001887"
     CDS             10871..11554
                     /locus_tag="LTU54_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL8296598.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            11701..12315
                     /locus_tag="LTU54_001888"
     CDS             11701..12315
                     /locus_tag="LTU54_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBL8296599.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            12331..12537
                     /locus_tag="LTU54_001889"
     CDS             12331..12537
                     /locus_tag="LTU54_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296600.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            13078..14208
                     /locus_tag="LTU54_001890"
     CDS             13078..14208
                     /locus_tag="LTU54_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HBL8296601.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            14300..15055
                     /locus_tag="LTU54_001891"
     CDS             14300..15055
                     /locus_tag="LTU54_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL8296602.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            15113..17152
                     /locus_tag="LTU54_001892"
     CDS             15113..17152
                     /locus_tag="LTU54_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBL8296603.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(17261..18412)
                     /locus_tag="LTU54_001893"
     CDS             complement(17261..18412)
                     /locus_tag="LTU54_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBL8296604.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(18566..19096)
                     /locus_tag="LTU54_001894"
     CDS             complement(18566..19096)
                     /locus_tag="LTU54_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL8296605.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(19195..19545)
                     /locus_tag="LTU54_001895"
     CDS             complement(19195..19545)
                     /locus_tag="LTU54_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HBL8296606.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(19702..21231)
                     /gene="ahpF"
                     /locus_tag="LTU54_001896"
     CDS             complement(19702..21231)
                     /gene="ahpF"
                     /locus_tag="LTU54_001896"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBL8296607.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(21241..21804)
                     /gene="ahpC"
                     /locus_tag="LTU54_001897"
     CDS             complement(21241..21804)
                     /gene="ahpC"
                     /locus_tag="LTU54_001897"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBL8296608.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         7438 a         3763 c         4364 g         6258 t
ORIGIN      
        1 tatattccta taaaagatat tatcctacac tttgaaaaat acgattatcg ttttcattgg
       61 aatagtgata attcagttat tgaaatttgg atacctattc aagattagaa gcaattttat
      121 atccatacac acaaagcagt tagtcttagg attgactgct ttttctatac agatttttaa
      181 tgacaggcaa ttatcaatta tggtagttgc cttttttgat tggaggaatt gaagaatgaa
      241 tgataaaaac tttattgaag aactaagaca aaagcgtgag gaatacggag taacacaaac
      301 aaggcttgct gttgcctgtg gtatcagccg tgaatattac aaccgcattg aaaaaggaaa
      361 acagccattg aatgacgaat taagagaaat catagaaaaa cagattgagc gtttcaatcc
      421 gcaagaacca ctattcttat tgattgatta ctttcgtgtc cgctttccta ctacggacgc
      481 attggcgatt atccgtgatg tattacaact gaaatctaat tacatgcttt atgaagatta
      541 tggaaaatac ggatatgaaa gcaaatatgt acttggcgac atcaatatca tgtgttccat
      601 gcaggagcat ttaggtgttt tattggaact gaaaggcaaa ggctgtcggc aaatggaatg
      661 ttatctgctg gcacaggaac gctcatggta tgacttcatg ctggattgta tgacggctgg
      721 tggtgtgatg aaacggcttg atttggctat caatgataaa gcaggaatat tggatattcc
      781 aaagttaaag gaaaaataca aggctggcga atgtatgtcc tacttccgta tgcagaaaga
      841 ttacagcggt acagaaaaat gcggtagcga tttaccaaag aatacaggag aaaccttata
      901 tctcggttca acaagtagcg aattatatat gtgtgcttat cagaaaaatt atgagcagta
      961 tgtcaagaat ggcacagaaa ttgaagatac ggagattaag aaccgttttg aaatacgcat
     1021 gaagaacgaa cgagcctatt atgcggttgt agatttactg acctatcggg acgctgaacg
     1081 caccgctttt tctatcatca atcattatgt ccgctttgtt gacagagagg acgacaagcc
     1141 aaaaagtcaa tggataacaa atgatgattg ggcatggttt gtaggggaaa acagagagcc
     1201 gatacgctta accacaaagc cagagcctta cactttacaa aaagcgttgc attggctaca
     1261 aagacaagtc gcaccaacca taaaaatggt acaggcatta gacagagaaa atcgtacaac
     1321 gatactgaaa gatatgattg agcaggcaga actaaaagac aagcataaac atttattaca
     1381 attagagaaa tcaaccatag aagaacgtat agataccgct gttccacaag agaatgacgg
     1441 tattttttaa tgtaattgaa aaatttcaga tttagtcagc aaagagctgt tttcattccc
     1501 tatgtgaagt gaaagaagaa aaataaattt tttattaaat ctgcaacaaa atggctactt
     1561 gcgtacagat atggtgcgag aaaaggaaat ctaaactttt tttgaaaata ggtcattaaa
     1621 tcggggcttg ctgtccctat atgaagtgaa agaagaaaaa agttttgaaa aaatgacgga
     1681 aattcgtgat tagtggacag tcttctgtga aaagaggaaa aacatcatca ttttgtattc
     1741 aaacaggcat tagcagttga gtgttgatgt gaaaagatga aaatactttg ttctcaactt
     1801 aacaatttaa ggtttccgtt ccctatatgg tgcaaagaaa atatttcatt ctattgttgg
     1861 cagtaacggt atggtttggg tagttagggt gtgaaaagga aaatattctt ttttcatcat
     1921 caacttagca aaatgacatt ttctatccct atatggtgca tgaaaagaag ttaaaatttt
     1981 tccatttaaa cttagcaaat ggacgtttgc tgtacagata gtagtgaaaa aggagaaaat
     2041 caaccgttga ttgccaaaat gaaccgttcc atttgtacta ataatggaaa aagttttgga
     2101 ttttggttac gtttctcctc ttttccaacg cgatatatag gaaagacgat ttttttcttt
     2161 caatcgttct ttgacaactg aataaagcta ttcaatacgt ttgacagaca gctagccgtt
     2221 gaaacaaata cgccacgacc tttcccctgc aagagcgagc gaaaacctat ttgtgttctt
     2281 gtaaaagatt tgtcctcttt tatcgccatg acctgtactg tcagataatg atactcccgt
     2341 acagccacag cttgagcatt aagagcgtcg cacgcaatgg gtacggctac ataagaacta
     2401 tgcagaggtg gaactcctgt gggctatgac aaaagccgtt gaattgctta gaaagatttt
     2461 acagaaaggg ggtatgtgtt attgataatg ctgtaaaaac aattcaaaag aaaaatggca
     2521 agtgtggcat tggcaacaga ggaaagacaa agattgtagt aaaagagcat ttttccgaaa
     2581 agggcaaaac aatggaagaa cttctaactg atgtcatgct ggaaaaagca aagcagacaa
     2641 tcgcataaaa tcggtgcagt tgtaagaaat agattgaatg acaaaaagta cccgtgttat
     2701 actttacttg caagcaggta ttgtaaacac gggttagtta taaaggagga taactgacaa
     2761 tgaaacaaca gatttacaat actgcacttt atcttaggtt aagccgagat gatgaattac
     2821 agggcgaaag ttccagcatt accacacaga gaagtatgtt gcgtttatat gcaaaagaac
     2881 atcatttgaa tgtcattgat gaatatattg atgacggctg gtcgggaaca aattttgaca
     2941 gaccgagttt tcaaagaatg attgaggata tagaggcagg aaaaatcaac tgtgttgtaa
     3001 cgaaagacct ttcccgtctt ggcagaaatt atattatgac aggacaatat acagaattgt
     3061 atttccctag tcataatgtc cgctacatag cgattgatga cggtgtagac agcgaaaaag
     3121 gcgaaagtga gattgcacca tttaagaaca tcatcaatga atgggtggca agagatacaa
     3181 gccgtaaagt caaatcagca tttaagacaa agtttgcgga gggggctcat tacggtgctt
     3241 atgctccgtt aggatataag aaacaccctg acatcaaagg aaaactgttg gttgatgatg
     3301 aaacaaaatg gattattgaa aaaatctttt ccctagccta tcaaggttac ggaagtgcaa
     3361 aaatcacaaa gcagttaaga gcagaaaaag ttccgacagc gtcatggcta aattttacaa
     3421 gatacggtac ttttgcacat atctttgagg gaaaacccga aagtaagcgt tatgagtgga
     3481 cgattgctca tgtcaaggcg atattgaaaa gtgaggttta tatcggaaac agtgttcaca
     3541 atatgcagtc tacggtatcg ttcaagagca aaaagaaagt gcgtaagccc gaaagtgaat
     3601 ggtttcgagt agaaaacact cacgagccga ttattgacaa ggaagtgttc tatcgtgtgc
     3661 aggagcagat aaaatcaaga cgcagacaga caaaggaaaa ggcaacgccg atttttgcag
     3721 ggcttgtcaa gtgtgcggat tgtggttggt ctatgagatt tggaacgaat aagacaaata
     3781 aaacgcctta cagttattat gcttgcagtt actacgggca gtttggcaaa ggtaattgtt
     3841 ctatgcacta cattcgttat gatgtgctgt atcaagccgt attggaacgc ttgcagtatt
     3901 gggctaaggc agtacagcag gacgaagaaa aggtattgaa caagatacag aaagctggca
     3961 atgcagagag aatacgggaa aagaagaaaa aggcaagtac attgaagaaa gccgagaaca
     4021 gacaaaatga gattgaccgt ttatttgcga aaatgtatga agatagagcc tgtgagaaga
     4081 taacagagcg aaattttgtg atgttgtcca gcaagtacca aaaagaacag atagaactgg
     4141 aacagcagat aacaagccta agagaagaac taagtaaaat ggaacaggat atgataggtg
     4201 ctgaaaagtg gattgagtta atcaaggaat attccgtacc aaaggaactg acagcaccat
     4261 tattaaatgc catgatagaa aaaatcctca tacatgaagc aacaacgaat gaggataacg
     4321 aaagaataca ggaaattgag atatattacc gatttattgg aagagttgag taatcaacaa
     4381 gagtaatatt tttaactaag ggaaaccggc ggtgtctata cttgggtcag tcgcacattg
     4441 ggaaaacgat tcggttttgc cgcaatcttt ttccaatggt ttgagataac agttggctac
     4501 ttgaccatgc tgtatttctt aactggtgca ttatcctatg caacgggtat tggtgctata
     4561 caaaacaata aatatctgaa attggcaatc ctattgatta ttttctgggc tattttactc
     4621 tcacagttaa aaggaaccaa atacacttca ttgattgctc ggatcggttt tgtagctggt
     4681 atcttgttgc cagcattagt cttgtttggc ttagggatcc attatgtcgc ttcaggcgct
     4741 ccactacaaa cgatactgtc tatgaaaaca ttgattcctg atttttcgaa attgcctacg
     4801 ctggttgttt ttgtttcctt tattttagca tatatgggcg tagaaacatc tgccagtcat
     4861 gcaaatgaaa tggaaaaccc taaaaaggat tatccattag caatgtttgt attggttatt
     4921 ctggcaatca ttttagatac ttttggcgga ttgaccgtcg ctactacagt acctcaacat
     4981 ggtttgtctc ttaatacagg ggtgattcaa tcgttagaat tcctattaag acacattgat
     5041 ccaaatcttg catggattgc gaaaattctt gcgattcttg tttgtttagg tgtcatcgga
     5101 gaaattgctt cttgggtaac ctctccttca aaagcacttc acgtagcagc agaagaagga
     5161 ttacttcctg aatattttgc aaaagaaaac acacacggtg ttcctgctca tttgatgatc
     5221 gcaaatggga tcgttgcaac aatctgggca gccgtcttta cattaagcgg cggggggaat
     5281 aatctttcat ttcttgcagc gatgtcattg actgttgtaa tctacctgat gttgtacttt
     5341 atgtttttcg cgggctattt gaagctggtt tttaaagatg acagtcttga aagagcctat
     5401 caggttccag gcggaaaagt tgtaaaagta atcgttgctt gtttaggtct tttgacttca
     5461 ctttttgctt tttgtatctc atttgttcca cctgctagta tcgcagctgg acagcatggg
     5521 atttatgaaa cgattctgat tgtttcttat cttatcgtct tagttattcc atttacgatc
     5581 tacgcatgcc gtcataaatt ccagcatgca gaaaaataaa aaatgaaagc tggttattca
     5641 aatggaagta aaagaattag aacaattgat tggaaaaaat ttccactatt atcaaacagg
     5701 acaagtggcg gattacattc cagcattagc tgaagtggat cccaaacaat taggtatggc
     5761 gatttatgat ttggatacaa aacaactgat tgagggagga gacgccgatg tgcgctttgc
     5821 catcgaaagc atgtcaaaag ttcccgtatt gttattagca atcaaagaca acggaataga
     5881 aaaagtcttt caaacaatca acacggaacc aaccggtttt gccttcaatt ctatcatgaa
     5941 tatggaaatc aatcatcgca aacatccaat gaatcctttt gttaatgcag gagcgattgc
     6001 aacaacctcg ttgattgccg gtaaagatgc agatgaaaaa tttgagcgaa tcttgtcatt
     6061 tatgaaagag atctgtgatg atcctaatat cacattgaat gaagaaatct atcattctga
     6121 atcaagaaca ggtgatatca atcgctctct tgcttactat atgaaaggta atcaaatgat
     6181 cgaaggagac gtaccggaga tactcgatgc ttatttcaga caatgctccc tcaatgtcac
     6241 cgctacaggc atagcaaaat tagctgctgt gctggcaaat aaaggaattg ctccttggaa
     6301 tggcaaacaa atcattactg aagaaagtgc tacaatcgtc aaatcaatta tgacaactgc
     6361 tggtttgtat gatgaatcag gagagttttc cgtccatgtt ggtgtacctg caaaaagcgg
     6421 tgtaggcggc gggttgatgg cagctgtgcc aaatcgttac ggcataggtg tcttcagccc
     6481 ggcactagat ccatttggta acagcgcagc tggtatccaa ttgttgaaag atgtagtcaa
     6541 agaactagac gctgacatct ttgaataacc attcttgaaa gaggctgggg taaaagtcca
     6601 aacggctcaa agctacctac aatgaggctg aaattgtgag cttgtttgac ttttgtccca
     6661 gtttcttttt ttattggttt ttgaaagggc ttttctaaat aaaaaggaaa caaaaatacg
     6721 atttacagat atgattaaat aaaatcataa gaaaaactgg aggcgtgtat caggaatgct
     6781 ggaaaattac atcgagcgaa atatatttag aaaagtgtat ctgtgtgaac aattatttga
     6841 attccaagaa atagacatcg aacaaacagc tatttctcta agggtgacta caccaacgat
     6901 ccttcacgat ctagaaagtc tagccgagtg tctagaatat tgtataaaag aacaagtaag
     6961 agagaagcat aagtataaac tcgtattcaa acatggtatt gctctttctg agctcacaca
     7021 gtttctgtac ggtcaatctt attttttaaa gtttttaagt tattatttta atggtatatt
     7081 cacgagtaca gaactggctg atcttgaatt tatttctttg agtaaagtat atacaatcaa
     7141 aaaaatcgta ttggattttt ttaaagaaaa tgattatttg aaaaataaag aaatcattat
     7201 cccagaattt gactcgcgga acatcttgct agctttagta cgttatgtca attgggaagg
     7261 ctatgaaaat aaaaatccgt atgtagaaga agcttgtaac gagttgattg attatgtaga
     7321 gactcatttt tttaaaagaa gatattcgga agatgaaaag tcctttatca tacgagaaat
     7381 agagatcgca atcggaagaa aggcatttcc ccttggactg aaagaagaag ataagaaaag
     7441 cgcagaaacg aagccgttat ttcaattagt ctgtcaagga ttggaagagc aaaaaggaat
     7501 tctagactta caagaagaag atgtctatta tattttttct ttgttcaaca gtagaaatta
     7561 taccaatgag aacatggagc tgttaagaaa agatttcgaa gttgtttata aaaattttgt
     7621 tctcaatgac ctatcactga atgaattgat tttacagatt attagtatcg ctgaaatcga
     7681 cgatgcagaa aattttattt tcaagcaatc ctttttacaa tttgtccgta cgttgtgggc
     7741 agatggacaa gtatttttac cggaaaggat ctatcttcta tcagaaaaag aacaacttct
     7801 gtacggaaaa attgtagaaa tattggaaga gtggaaaaag aaaaatcaaa tccaactaag
     7861 atggaatgaa aatttcattc gaaaattcgt caaaagtctt tctttgatca acagtgaaca
     7921 agcggggaga caggaaatag aaatttttgt tgtttcagaa agttcagtca aacaattttt
     7981 ttatcgaacc caatttcatt tgtatgcgga agaaggcatc gctgaaataa atcctatttt
     8041 atttcgttca ttagaagaat tgcctgatga atgtctatgt gcgaaaaaaa gagtggtttt
     8101 gtgcgatgct gctagctatc aaaatggatt agaaactgaa aagacaaaga tctatccaat
     8161 ctcattaaaa ggtgcaaaca tccatttgta tcatattgct caggaacttc acctattaaa
     8221 aaaacatatt tcttgatgag accttaatta ggagaccttc tacaaagctt tttaattgtc
     8281 tgtagaaggt ttttatttga gtcctgattc gtgcgaagat ctacttatat aacaagcttt
     8341 ctctgaaatt tttttaaaat gctattgact tagagtcaac tctaagtagt agaatagata
     8401 aagtgtaaca aatctatttt cattaaaaga agatagtacg gaggagagaa tgtattatga
     8461 gtacagtatt acatgtattg gatgcaatca atagccccgc agatgtcaaa aaattatctg
     8521 ttgaggaaat gaaacagttg gcagaagaga tgcgccaaaa aatcttgcaa aaggacagcg
     8581 atgtaggtgg gcacgttggt ccgaatctag gtgtagtaga attaacgatt gcgtttcatt
     8641 atgtatttga ttcaccaaag gataaaatcg tttgggatgt ttcccatcag tcttatggtc
     8701 ataagctttt aactggtcga aaaaatggtt ttgaaaaagg acattaccgt gaagtgagcg
     8761 gatattccaa ccaacatgaa agtaaacatg actattttac agtggggcac acatctacat
     8821 ctatcagctt agcaacaggt ttagctaaag cgagagatct gaagaaagaa tcaggaaatg
     8881 tggtagcatt tatcggtgac ggctccctaa gtggtggctt agcttttgaa ggattgaaca
     8941 atggtggtgt tttacaatcc aatctaatcg tattagtaaa cgataatgag atgtctattg
     9001 atgagaatca tggcagttta tataaaaact tagctgagct tcgtcaaacc aacggtaaaa
     9061 gcagcaataa cctatttaaa gcaatgggat ttgattatcg ttacgtggat aatggaaacg
     9121 atctggaaac ggtgatccgt ttatttgaag aagtaaaaga tattgatcat ccgattgtac
     9181 ttcacgtcca tacggaaaaa ggtcgcggtt atcagccggc aatcgataat aaaatgaaat
     9241 atcattggca tgtaccattt gatctgtcca ccggggaggc aaagcaagta acttctggag
     9301 aaacatttaa tatgatcatc atggattatt tggataagaa aatgcaaaca agtacagctc
     9361 ctattgccgc agtcaatgca gcgataccag gcgcctttgg actgaaaaca ttccaagaaa
     9421 aacatccaga tcattatttt gacgtgggga tcgctgaaca gcactctatc actttcgctt
     9481 ctgctttggc ttctcaggga gtacgtccag tcgtcttcca atcaagtact ttcttgcaac
     9541 gggcatatga tcaattatca catgatttag cgatcaacga gaatccagtg attattttag
     9601 tcggtggcgg aacgatctca gctagtgacc gaacacacca aggaacattt gacattcctt
     9661 acttatcaag tatcccgaac atcaaatatc ttgctccagc aacaaaagag gatatgatct
     9721 ctatgctaga ttgggcgttc gaacaaacag cgcagcctgt agcaatccga attcctacac
     9781 atggtgtaga acatggtaca gctatcgcaa atgattatag tattccagcc tatcaaacag
     9841 taaataatgg aggaaaagta gcaattttag ctttaggcgg ttttcttgaa ttaggaaaag
     9901 aaacagtaaa agcttttaga aaaaaaggaa tcgaagcaac gttgatcaat ccaggaacga
     9961 tcacagaaaa tgacgtagaa acattagata aattaaaaga aacacacgaa ttagtcatca
    10021 cattagaaga tggtagtttg tcaggaggat ttggcgaaaa tatcagtcgt tattatggtg
    10081 attcttcaat gaacgtatta aattttggtg cagagaaaga attcacggat agcgttcctg
    10141 tagggcagct ttatgaacgt tatcatttaa caccaaaaca aatcgttgaa gatagtctgc
    10201 gtgtcttgaa tattcaataa ttcattatgc tcaccatcca gataattgtt tatactagta
    10261 tctggatggt tttttcatta taaaaagcaa aggggtggat gttatgtatc agcaagtaga
    10321 agaatatgtc aaaaagttcc aagaaaaata tcctagttat caatcagaaa aattaggttt
    10381 cttttcattt ggggatacag aaaaaatggc agacgagttg agcgaactag ttgtaaaaaa
    10441 gataaagact ggtacatcaa gtggttacga tctgtataaa caagagaatg agcctttacc
    10501 aaaagttggt caattagacg tggtcctgaa tggaaaaaat gaaccagttt gtatcatcaa
    10561 aaatataaat gtgaccatct tgccttttag tgaagtcacg aaagagcatg cttggaaaga
    10621 aggagaaggg gatcggacat tagcttattg gcggaaagtc catgctgagt tttttaaaca
    10681 tgcttataaa gaagcgttga acatagaatt tacagaagag aagctggtcg tatacgaaga
    10741 atttgaagta attttctttt agtaagaaag gtctgctgaa agaaatagct cattattgtc
    10801 tcctgaatag aaaagggtta catttttata tgaagctgta tttttatata acattactgg
    10861 aggagatatt gtggatacac tggaacaaat caaaggcgga gtcgtcgttt cttgtcaagc
    10921 tttggaaaac gagccgctac acagttcttt tattatgggg agaatggctt tggctgcaaa
    10981 agaaggcgga gcagttggta ttcgagcaaa tacgacagaa gatatcaatg aaatcaagcg
    11041 aatcgttgat ttaccggtaa tagggattat caaaagagat tacgatgggt cagaagtgtt
    11101 tattactgcg acagaaaaag aagtggaaga acttttggag acgcaagcag aaatcattgc
    11161 actggatgca acttctcgta aacgtccgac agaaaaaaca actgctgaac ttgtccaaat
    11221 gattcacgaa cacggtcggt tagctatggc agatatttcg acttttgagg aagcgcttca
    11281 agcgcaagaa gatggttttg atcttttgtc tacgacttta gcaggctata cagaatattc
    11341 acgaaagacg cagacacctg attttgattt attgaatcaa atcatagaac aagtcgatat
    11401 cccagtaatt atggaaggac atactgactc tcctgaacaa gtagagaaag catatgaatt
    11461 aggtgctttt tcagtcgtag ttggatctat tatcacccgt ccgcaattga tcactaaaag
    11521 atatgtcgaa gccgcaagta aagcaaataa ctaaaaaaca cgcacggcac agattcttga
    11581 caaaaaagaa aaattgatag atagtttaaa gcagattcta agaagatgaa aaaagcatta
    11641 cttttcattt tgtcttcaag atctgctttt taacgaattt tatagaaaga aggcgaaacc
    11701 atgtttgaac ggagtccttc agaattgaca gaacgtgaaa attacaagtt gctgattggt
    11761 tcagtcatcc ctagaccagt agcagtagta acgactaaat cagcaaaaga tatagtgaat
    11821 atcgcaccct tcagttactt caatatcgtc agttcgaatc gacccatctt atccttggcg
    11881 atccagagaa aacaagatgc agtaaaagac acagcaaaga atattcttga gacaagagaa
    11941 gctgttatcc atatccttga tgaggacaac gtagaagaag taaataaaac agcagccaat
    12001 ctgcctaatg aggaaagcga actgtacctg actgacttga ctttaactga ttcagtaatc
    12061 atttcagtac cagcattgac agaagcaaaa gttcgttttg aaacatcctt atatgaacat
    12121 gtcgagatac ggaataaaaa taaagtaaca gctgatttat ttttgctaga agtacagaaa
    12181 tatcatttaa atgaaacagt ctatgatgat aagacaggaa ggatcgatcc aagaaaattg
    12241 aatgctgtaa gccgtttggc aggaaatgat tatgcagcaa taggagaaat tttcacgatt
    12301 gcacgtcccg aataaaaagg agcactagtt atgttaaaaa taagctataa accatcgaca
    12361 gattcaaaag aaatgaaaaa agaatatgaa acagtgaatg attttctgca agggcagtac
    12421 ttagaagttc cgccattgca agaccatttt gtcgttacta ctgttacttt agatggtaag
    12481 gaaatagaaa tgccagatca aacgattagc ggattgttta attactttaa caaataaata
    12541 tttattccga aaagtgaaaa aacatataaa aaaacacata aaactaaaaa attatcaaag
    12601 aatcctggtg caactgaaag aatttgcaac gggattcttt caacttattt gttatttttt
    12661 tctaaaaaat attaaagatt gttaaatttc actatttcat ttcaaattcc attgcctaat
    12721 cattaggaaa agtctatcat gaactcgtaa atagaatata gtaataacct tgttaggtga
    12781 ggctcctata caaacatagg ctgttgccca gaaacgtcga aagtcgccaa tgggtaaaac
    12841 agaaactgtt ggattaaggc tttttccaat agctaagaat gttttcttta cgttgtatag
    12901 tgctaaaact cggacgatga gatgatgcta ggtcagaatt cttgttcgaa aaggtttctg
    12961 accttttctt tatatttttg tgactgactt atttgatgag acaaactcgc tcctcacctg
    13021 acaagtggat tgctgtgtga ggaggagatg cggtgaaaaa agatcgattg gtggattttg
    13081 ggtccttatt tgactttgtc catgatcggt cttttagaag tatacagtgc cagttcttat
    13141 cgtttattgc aagccgatga aaatacaaaa agtctattat tgcgtcaact aattttcata
    13201 tttttgagtt ggagcgtgat cttcttagct cgttcagtca aactacacta tttacttcac
    13261 cctaaaatag caggatacgg tttagcctta tcgattttct ttttagtatt agtaagaata
    13321 gggatattcg gtgtcactgt caacggcgca caacgttgga tctctctgtt tggcattcaa
    13381 ttccagcctt ctgaactggc aaatcttttt ttgatttttt atttaagctg gttttttcgt
    13441 gacggaaata gtagcccaaa agatctaaaa aaaccattcc tgattacagt aggtataact
    13501 tttctgattt tatttcagcc aaagattgct ggagcattga tgatcctttc gattgcgtgg
    13561 gtcatatttt gggcagcggc ggttccattt aaaaaaggga tctatctaat cgttactttt
    13621 tctgcattgc tgattggagc agcaggcggg gtattatatt taggaaataa aggttggctt
    13681 ccacagatgt ttaatcatgc ttatgaaagg atcgcgactt taagagattc ttttatcgat
    13741 agtcatggtg cgggctatca aatgacccat tcgttttatg cactatataa tggtggcata
    13801 tttgggcggg gattaggaaa tagtatcaca aaaaaagggt atcttccaga gacagaaaca
    13861 gattttattt tttcgattat tacagaagag cttgggctaa tcggtgcatt gtgtgtgctt
    13921 ttcttattgt tctcattatg catgagaatc ttttgtttaa gcagtcgctg taaaaatcag
    13981 caagctggat tgtttctttt aggattcgga actttgctat tcgttcagac gataatgaac
    14041 gtgggaagta tcgcggggct gatgccaatg actggtgtgc cattgccatt tgttagctac
    14101 ggaggaacaa gttatctgat cctttcttta ggaatcggaa tcacgttgaa tatttcttca
    14161 aagatacagg ccgaagagtt ggcgctctat cgacctgaaa aacaataaag agaaactgag
    14221 aaactttcag tttgaattat cataaagtac aatcttttat ttttcaagcg agaggttctt
    14281 ttagaaagag gaattcgcaa tgaaagtatc tcaaaaaatc atattatcta ttttgttact
    14341 gcttacatta gtagttggct atttttcgat tgagtttatc catggatttt cgtctgcaaa
    14401 acagagttca actgtgaaaa aggtagagcc gaaaagtatc cctaccacac taaatgtggc
    14461 tttgattggt tcagatgctc gttcgaaaga cgaaaacggt cgctcggatt cacttctggt
    14521 tgcacaatac aaccagaaaa cacaacaagc aaaactgatc tctatcatga gagattcata
    14581 tgtcgatatt ccaggttacg gaatgaataa gataaatgca gcttactctt atgggggagt
    14641 tgatttatta aatcaaacat taaaggaaaa tttcaaattt gaagctccct attatgcaag
    14701 tatcacattt caagatttta tcgattgcgt taatgaactg tttcctggtg gagtcaagat
    14761 cgatgcagaa aaatctctag atttagatgg cgtatatata aacaaaggag agcaagtgat
    14821 ggatggtaat actttactcc agtatgctcg atttcgggaa gacaaagaag gagacttcgg
    14881 gaggatcaga aggcagcagc aagtgatcaa agctatctct cagcagctga aagacgtaac
    14941 ttcaatattc aagctaccca aagcggttgg gaaattactt ggtagtatcc aaacgaacct
    15001 tccagaaagt gtgcttctgg attgtggtat ggatttcctc aaagacatat gttagaataa
    15061 acaggtataa atagtgaaat aaaggaatga caagcaagag aaggaggaaa aaatgaaaag
    15121 aagtgacaag cacggcaaaa atcgaacagg cgcttatatt gccggcgcag tgatcttaat
    15181 agcagctgcg ggtggcggtt atttttacta ccagcactat caagaaaccc aagcagtaga
    15241 agctggagaa aagacagttg agcaatttgt ccaagctttg aacaagggag attataacaa
    15301 agctgcagaa atgacatcaa aaaaggcggc aaataaaagt gcattatctg aaaaagagat
    15361 cttagataaa taccagaata tatacggtgc tgctgatgtc aaaggacttc agatatcgaa
    15421 tctaaaagta gataaaaaag acgattctac ttacagtttt tcatataaag caaagatgaa
    15481 tacctcatta ggtgaattga aagatctttc ttataaagga acattagaca gaaatgatgg
    15541 tcaaaccacg atcaactggc aacccaactt ggtttttcca gaaatggaag gaaatgacaa
    15601 agtaagcctg accacacaag aagcagcaag agggaatatt atagatcgaa atggtgaacc
    15661 attagcaaca acaggcaaac taaaacaatt aggagtcgtt ccaagtaaac ttggagatgg
    15721 aggcgaaaaa acagccaata tcaaagcgat tgcttcctcc ttcgacttaa cagaagatgc
    15781 tatcaatcag gcgatttcac aaagctgggt acaacccgat tactttgtcc cattgaaaat
    15841 cattgatgga gcaacgccag aacttccagc tggagctacc attcaagaag tagacggcag
    15901 atattatcct ttgggtgaag cagctgctca gctgattggt tacgtgggag atatcacagc
    15961 agaagatatt gataagaatc cagaattaag cagtaatgga aaaatcggtc gctcaggttt
    16021 ggagatggct tttgataagg atcttcgtgg gacaacaggt gggaaattaa gcattacaga
    16081 tgcagacggt gtagagaaaa aagttctgat cgaacatgaa gttcaaaatg gcaaagatat
    16141 caaattgaca atcgatgcga aggcacaaaa aacagctttt gacagtctag gaggaaaagc
    16201 tggatcaact gttgcgacaa cgccaaaaac cggtgatctt cttgcgcttg ctagctctcc
    16261 aagctatgat ccaaacaaaa tgacaaacgg gatctcacaa gaagattaca aagcttatga
    16321 agaaaatcct gaacatccat tcatcagccg atttgcgaca ggttatgctc ctggatctac
    16381 gtttaaaatg attacagcag caatcggtct cgacaacggc actatcgatc caaatgaagt
    16441 gttgacgatc aacgggctta aatggcaaaa agacagttct tggggatctt atcaagcaac
    16501 gcgtgtcagt agtgatgtat cacaagtaga cttaaaaact gctttgatct attccgataa
    16561 tatatatgcg gcacaagaaa cgttgaaaat gggtgagaaa aaatttcgta caggcttaga
    16621 taaattcatt tttggtgaag accttgattt gccaattagt atgaatccag cacaaatttc
    16681 taatgaagat agctttaact cagatatctt gctagctgat actggatatg gacagggcga
    16741 acttctaatt actcctatcc agcaagcagc aatgtattct gtttttacca acaatggcac
    16801 acttgtctat cctaaattga ttgcagataa agagacaaaa gataagaaga atgtcatcag
    16861 cgaaacagca gtacaaacga tcgtgccaga tctgagagaa gttgtgcaag atgtaaatgg
    16921 tacagcacat tctctttctg ctttagggat tccgttggca gcgaaaactg gtacagcgga
    16981 aatcaaagaa aaacaggatg taaaggggca agagaacagt ttcttgtttg ctttcaatct
    17041 tgataaccaa ggttatatga tggtcagcat gttggaaaat aaagaagatg atgattcagc
    17101 aaccaaacga gcatcggaac tattgcaata cctcaaccaa aattatcaat aaaaaattgc
    17161 tttttaagta cagtaaaagg actgtggcgt tatcgtcaca gtcctttttt caaataaacg
    17221 gttttcacga tgtctctttc acaacttctt tttgatttac tcacacttca taaacttttt
    17281 ttgtaaaata tttgagaaca agtggagaaa taagtgtact taataaaatc acgataacta
    17341 aaggtgaata gtactgtggt tcgatcaatt gactttgctg tccaatttgt aaaatgatca
    17401 aagccatttc tccacgcgaa atcataccag caccgaccat caatgaacta tgttgagaaa
    17461 aaccggcaat ctgagcacca aaataacctc caagcaattt ggtcaaaatc gctacaagcg
    17521 tcaatataga aataaagagt agttgttcac taaagtcaga aaaatcaact tctaatccta
    17581 cactgacaaa aaatactggt ataaatactg catatcctaa agcctctaca ttgtggtata
    17641 cttcttcttt aacctttgtc tgactgacag ctatcccagc aaaaaaagca ccaatgacag
    17701 agctaagccc aacaagatcc gctacgtaag acattcctaa acaaataact agcgatacga
    17761 tgataataga agaatttgcg tacacttttt ctgcaaatga aagtaggaaa ggcgcaatcc
    17821 atttgactaa taagaaaata aaaacaaaat aaattagctg ttcaatcaaa acaagcggta
    17881 aagaagcatt gctagaagct tcccccgtta gaaaagatag gctgaaactt agaactaaaa
    17941 cgactaaaat gtcatctacc acagaagcac ccaaaattgt tgatccttct tttgtattca
    18001 ctacgtttag ttcttttaat acttctactg aaatactgac agaagttgcc gcaagaataa
    18061 tcccaaagaa aatcgcttca tttcccgaaa cttggaatgc tttgcctgtc aaccagccaa
    18121 aaacaaccgg gaagatgatc ccaagcaaag caacgaacat accaggtcgg aaatattttt
    18181 tcaaaagaga taaatcactt tccagaccag ctaaaaacat taataagatc acaccaatct
    18241 ccgaaaagtc atggactaga atatcaggtt tgacccaatt caatccagct tggcctagca
    18301 aaacaccaac aagtaattgt ccaatcactg ctgggatacc taatctccgc gagatatgtg
    18361 agccaatcgt tgttgaaatc aatatcaggc ataaaatacc aataaattcc attctcatca
    18421 ttcctttgta taaaatatag cccgaactta gcataacata cattattgaa aaaataggaa
    18481 aatatactca caaaaaaacc aacaatccaa aagatttttt tggattgttg gtttttatac
    18541 ggtctatttt aataactaaa aatgattaac gtatagtcat ttttcttatt ctgatgaaat
    18601 tgataatctc ctggatgccc atgaaaatca aaataaagcc gaatatttgg aatagaagca
    18661 tcaaactttt gaatggattg aacaataaga cgaatccgcc aatcaacaac agaatactac
    18721 tataaacact ccaactttta gcttttgtat tcatgctgaa gaacaactgg aacaagctgt
    18781 tgatgataac agctaatcca agaagaatcg gcaggataga aacgattgca ggtgcaaaag
    18841 catagacgat cagagctaag atcaggaaaa aaagaccact gaacagaccg atactccaag
    18901 aacctgtttt ccttttaaaa cggtaagcct caaaaagatt caacaaccca agcaaaacaa
    18961 gataggcggt gatcagataa ccgataaaat gaaaaacagc agaagggttt agcacaatcg
    19021 caatcccaga aataatataa acagcagctc ttaataaggc gtatttttga atattttgtt
    19081 taatcgtctc aaacatataa gaaaacctcc tttgacttat tttaacgtga actagtcatt
    19141 ttaccaaatt aaatgttaca aaatgagcga aggagaagaa tatctctgga aaaatcaact
    19201 tctgaggatc ttttccattt ttttgccttt tgctagctca tcgatcattt tatctaaata
    19261 acgaatttct ttcatcaagt tatcctcgat gtcttctact cgtacaccac aaacaacacc
    19321 tgtaatcaag gaacgtgaag gattggggac aggggcttcc gcaaaaaatt gttcgaaagt
    19381 cacttgtttt tgtagttgat attctagttc tgtttgggaa taacccgtca gccaacaaat
    19441 gatttcatct acttcctctt ttatacgatc ttttctctcc gcttttgcta catacatagg
    19501 ataaacgctt gcaaaacttg tcgtaaaaat acgattttgc gccataatca gtcacctttc
    19561 tttttttcaa attatttact acttatagta tataagaaaa agagaagaaa aagcgattat
    19621 tcaaaaaaac aaaaaggatt gtgacaaaat tcttgtcacc atcctttttt ccgctctctg
    19681 actttttaca atcttcatag ctcaagtgcg catcagataa tcgaatgctc ctaaagctgc
    19741 agtagctcct gaacccattg aaacaatgat ttgtttgtat ggactatctg tacaatcacc
    19801 agctgcgaaa acgccgggga tattcgttgc gccatttttc tcggtcacta tttcatttct
    19861 tggattcatt tcgatcgttc catctagcca atccgtatta gggagtaagc cgactagaat
    19921 gaatactcct gaaacagcta gtgtatgcat ctccatcgta cggcgatcga tataggagag
    19981 agattctacc tgattcgtac cgctgatttc tttcgtcgct gcatgtttga taacagtgac
    20041 attcgcaaga gagactagtt tatcctgtaa gatttgatct gcttttaatt ctggtaagaa
    20101 ttctagaaca tatacatgtt ttgctaaccc agccaaatcg attgctgctt caacaccaga
    20161 attaccacca ccgataacag caatctcttt tcctttaaat aaagggccgt cacagtgtgg
    20221 gcaataagca attcctttat ttttaaactc tttttcgcct ggtacattga tagaacgcca
    20281 gtgcgcacct gtcgcgatga tggcggtttt tgctttaagt gatataccgt tagctaagcc
    20341 aatattaata aaatgttgct ctttatttaa ggaaacggcc tgttgattca tcataatgtc
    20401 gatagggtaa ctacgcacat gactttctac ttgctgcatc aattttggac cttctgtgta
    20461 agaggtacca atcatatttt caatccctag tgtttcaacg acttgtccgc cgaaagaatc
    20521 agcgacaagt cctgtacgga ttcctttacg tgctgcatag atagctgcac tactagctgc
    20581 tggtccaccg ccaatcacta atacatcata aggttctttt tcaaacaact cttcttcaat
    20641 caaagggcca gtgatttttt caacaatttg ttcgatcgtc atgcgtccgc tactaaattc
    20701 ttctccattt aaaaacacag ttggcactgc catgattttt ttatcatcga tttctttttg
    20761 gaacatccca ccttcaatca tcgtatgagt aatatttgga tttaatacag ctaatatatt
    20821 caatgcctgt acaacgtcgg ggcaattatg acaagtcaag ctaacataac tgtcaaaatg
    20881 caatgtttgt gttatttttt tgattttaag actcaaagat tcactgattt tcggtgcccg
    20941 tccactaatt tgtaacatag ccagtaaaaa agaggaaaat tcatgaccga gggggatacc
    21001 agcaaaaacg attccacttt ctttgccaac tggattgata gaaaaactag gtgtgcgcat
    21061 cgaatctctt ttttcccaag agattttagg agacatttct gctatttcct tcgtaaattc
    21121 agctagtttt tgactatttt cactattatc caaactaaca gaaaaaacaa taggagattc
    21181 aatcagtgct aaatattgtt cgagattttg tttagtttct ttgtctaaca tatccaccac
    21241 ctaaatcttt ccaacaagat caaaacttgg tttcaatgtt tcgccacttt ctttccattt
    21301 tgccggacaa acttcacctg ggtgggtacg tacatattga gcagcgcgaa tcttatcaat
    21361 cagtgcacta gcgtcacgac cgattccatc tgcattgatt tccatagctt gaacgattcc
    21421 gtctggatca ataataaatg ttcctcgctg agctaatcct tgctcttcat ccaaaacatc
    21481 aaaaatacga gaaatctgat gagacggatc accgatcatc acgtattcga ttttaccgat
    21541 agcatctgat gtatcatgcc atgctttgtg agtaaagtgg gtatcggtag agcaagaaaa
    21601 gacttcaaca cctaaagatt tcaatgtact gtattgttct tgtaagtctt ctaattcagt
    21661 aggacaaaca aaagtaaaat cagctggata gaagcaaaga atactccagt tccccataag
    21721 atctttttca ctgacttcta aaaattctcc ttgatgataa gcatgtgcat taaattcttc
    21781 gatttttttc ccaatcaatg acatagtaaa acctccaata ata
//
LOCUS       DAFGZE010000048        21689 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.049, whole genome shotgun sequence.
ACCESSION   DAFGZE010000048 DAFGZE010000000
VERSION     DAFGZE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.049"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            61..813
                     /locus_tag="LTU54_001898"
     CDS             61..813
                     /locus_tag="LTU54_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296609.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            829..1590
                     /locus_tag="LTU54_001899"
     CDS             829..1590
                     /locus_tag="LTU54_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296610.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFSVYDVSDILQKMDVKDLTTDQIESQLKDRVKKLSSDQLKLISNGETKTIDQQT
                     GVFEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSE
                     ASQPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            1603..1863
                     /locus_tag="LTU54_001900"
     CDS             1603..1863
                     /locus_tag="LTU54_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296611.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            1860..3950
                     /locus_tag="LTU54_001901"
     CDS             1860..3950
                     /locus_tag="LTU54_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL8296612.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLNNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTILAVSLLL
                     LVLATFGYAVLKFN"
     gene            complement(3986..6043)
                     /locus_tag="LTU54_001902"
     CDS             complement(3986..6043)
                     /locus_tag="LTU54_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL8296613.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(6188..6379)
                     /locus_tag="LTU54_001903"
     CDS             complement(6188..6379)
                     /locus_tag="LTU54_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296614.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            6567..7022
                     /locus_tag="LTU54_001904"
     CDS             6567..7022
                     /locus_tag="LTU54_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296615.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            7019..7261
                     /locus_tag="LTU54_001905"
     CDS             7019..7261
                     /locus_tag="LTU54_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HBL8296616.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            7279..7581
                     /locus_tag="LTU54_001906"
     CDS             7279..7581
                     /locus_tag="LTU54_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296617.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            7653..9707
                     /locus_tag="LTU54_001907"
     CDS             7653..9707
                     /locus_tag="LTU54_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296618.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            9707..12319
                     /locus_tag="LTU54_001908"
     CDS             9707..12319
                     /locus_tag="LTU54_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBL8296619.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            12316..14847
                     /locus_tag="LTU54_001909"
     CDS             12316..14847
                     /locus_tag="LTU54_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296620.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PEQAKGMAGNDKSGLSDIQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            14899..15231
                     /locus_tag="LTU54_001910"
     CDS             14899..15231
                     /locus_tag="LTU54_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296621.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            15232..15825
                     /locus_tag="LTU54_001911"
     CDS             15232..15825
                     /locus_tag="LTU54_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296622.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            15988..17682
                     /locus_tag="LTU54_001912"
     CDS             15988..17682
                     /locus_tag="LTU54_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBL8296623.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEIVKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            18370..20247
                     /gene="ltrA"
                     /locus_tag="LTU54_001913"
     CDS             18370..20247
                     /gene="ltrA"
                     /locus_tag="LTU54_001913"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL8296624.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            20378..20755
                     /locus_tag="LTU54_001914"
                     /pseudo
     CDS             20378..20755
                     /locus_tag="LTU54_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            20755..>21689
                     /locus_tag="LTU54_001915"
     CDS             20755..>21689
                     /locus_tag="LTU54_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL8296625.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVG"
BASE COUNT         7710 a         3493 c         4461 g         6025 t
ORIGIN      
        1 ggggcaggcg caatttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta
       61 atgattatca gacaccctaa gaaaaagcga atcatgggta aatggatcat tgctttctgg
      121 ttactttcag ctgttggtgt cttacttttg atgcctgctg aagcgagcgt ggcgaaatac
      181 caacaaaatc agcagattgc cgcaatcgat cgaacaggaa cggcagcaga aacggattct
      241 tctttggatg ttgctaagat tgagttaggc gatcctgttg gtattcttac aattccaagt
      301 atttctttaa aactaccaat ttatgacgga acttccgaca aaattttaga gaatggtgta
      361 gggattacgg aaggaactgg cgatattaca ggaggaaacg gtaaaaatcc tttaatcgca
      421 gggcacagtg gactttataa agataatctt ttcgatgatt tgccttcagt taaaaaggga
      481 gaaaagtttt atatcaaagt tgatggtgag cagcacgctt atcaaatcga tcggattgag
      541 gaagtgcaaa aagacgaact acaaagaaac tttgtcactt acttagaacc aaatcccaat
      601 gaagatcgag taacgctaat gacatgtacg cctaagggga tcaacacaca ccgtttccta
      661 gtgtatggga agcgagtaac ctttacaaaa tcagaattaa aagatgaaga aaataagaaa
      721 caaaaattat cttggaaatg gcttttagga agtactgttt ttctttcagt catgatcatt
      781 ggttcgctat ttgtttataa aaagaaaaaa taaaggaggg taaccaacat gagaaataga
      841 aaatggctat ttgtttgtgc agccgctctt ttttctttca ctgcctacac acagttgacg
      901 tttgcagaca gacaagaaag cacggatagc acagaacaaa caatccaaac gtcagatagc
      961 agcacgattc aaagcagcga tgaaaccgtc gattcgtcaa cagttgattc ttcagaagaa
     1021 cctaaaattg ctgaagacga tcaaatagtc aaacagacca ttcacgttca aaaaattatt
     1081 tcttccgaac aattagacaa ccaggggcaa aagcttgaaa aacaagaagg agtaaatggc
     1141 gcgaagtttt ctgtttatga tgtatcggac attttacaaa agatggatgt taaggatctc
     1201 actactgacc aaattgaatc ccaattaaaa gatcgagtga aaaaactatc cagtgatcaa
     1261 ctgaagctaa tttctaatgg agaaacaaag acaattgatc aacaaacagg tgtctttgaa
     1321 ttctcggtag aagtacaggc aaatcagaaa caagcttatt atattgtcaa tgaatcaagt
     1381 cctgaaaaca tttctaatag cgaggatatt ttgctattaa cacctgtttc tgataaaaat
     1441 ggtgaattct taaaggatgt gtggatttat cctaaatcag aagcttcgca acctaaagaa
     1501 gaagtaaaaa aaatcgtatc tactggcgta aagaaaaact tctttgagaa ttgttgggat
     1561 tttgttactc acttgttttc tagagattaa ggaagaaata aaatgaaact gatcaaatta
     1621 ttattattca atttttcttg tttaaccgca gtctttttgt actgcggttt attattttct
     1681 aaactatcta ttttgccaag ctaccaacta tacctaatga ggattttaaa cataccagct
     1741 gcttccatgc cgctagatat tatggaaaag ctcattagga atccattatt tactacgatc
     1801 aatttgatcg ttattgtcgg aatttcacta gtcatttata aaaaagaggg gatgaaggga
     1861 tgaattttaa acactcgtgg acaagacggt taaaatatgt ttttcccata atgatgatgt
     1921 taggtacatt gtttgggtta aaaacagcca atgtctttgc tgaaaaagtt attgtcgatc
     1981 cgtcaaatcc aatccagaac gtaaagaata attttattat tccaaaagat gttcctaatg
     2041 caaaaacaaa tattactgta aacggagcgt caaaagttta caaatatcat gttgatgcca
     2101 acaaaggtgg ttttacagac gactacgtag gattagtgga gggaaacaaa aatatacgct
     2161 atccttcttt ccaaaaagaa tacggtgaaa caaaccttgt tttagaaggc gtaacaacca
     2221 acaccaaagt caatattgac tatggaaaaa tcggtacgta taacgggaaa aaagtcaata
     2281 tcaagctagt tctatctaat attcacttat actcggatac gttgccgtgg aatctactag
     2341 ataacaacta tacaaaaact cattttcgag acgatggcta taaaaatacg aatggtgcaa
     2401 tgagtaaatc taaaaaaaga acagtacttt ggatctcaga caatcttttc tcaggaattg
     2461 tttaccattc aacacaaatg aatgtgcagc tagtagccac atatgaggac ggtagccccg
     2521 ttcaattttc aggagataca tttatcagtt ttaactcttt gaaccctgca ggaggaaaaa
     2581 gcacggatct taaaggagaa tatgcccact atgacaaaat gaatacgaca gattggtatg
     2641 tcagaaaaga tacggttctt tcagaattta aatctttcta caacaatctg aacgtggtag
     2701 gtggccaccc aggaggaagc tctaagctga cacaagcgga taatgatttc aataatttgc
     2761 atgataaatt aggagatccg aagtttggtc aaggtactgt gtcctttaaa atatctgaag
     2821 caaatccgac gtttgtgatt ggttcttcta atgttcaaac ctggtttact ttatcttctg
     2881 cgacaatctt tagtgttgtt cctgatcaac ctgaaaaaac aggggttgat aaaaatggaa
     2941 ataacgtgaa cgataaaatg ttgcaagtag gagacacgat tcaatatcga atcaaacaga
     3001 aggtgaatcg attaggagtg gatttactag cggtttacga tcgatttgag ttaatcgata
     3061 atttaccaaa agaagtaaat tacgtcgatg cacacgttga aagtggtaca aataaaaaat
     3121 tcgatgtatc tggtgaagtg acctatgaca aaacaaaaca ccaggtaaaa tatgcagcga
     3181 aagctgatac attaaaaaat cgaatgaagt ataacggcga aacttacgaa ttagtgatca
     3241 atgtaaaagt caatgaattg gccaatcaaa atagtgtagc caaaaatcag ggaacgtcga
     3301 tcattaataa agtagaaaag gatacaaata tcattaccgt ttatttccct aaaattccag
     3361 tgaaagaagt acaacaaaat ggaaaagatg taaacggacg aaatgacggt aagaagggta
     3421 cgccgacagc tcctttaaac gcaggatctg aagtacaata tctagtcaca caaaaatggc
     3481 acacaaaagg cgtagacgct gcgtcagatc actacaaaca atttagtatt caagatccaa
     3541 ttgaagctcg tttgacatat aaagaaggtt ctgcgcaagt aatagataag agtaaaggaa
     3601 aagatattac ttctgaaggc acactaacct atgatagcaa ttcaagaacg ctgaagtggg
     3661 aagcttccgc tgacttctta aataacaatc ttttagatgg acgagaaatt cagctgatat
     3721 ttacagctaa aactccatta caatcagaaa aaaatattga taaccaagcc gtagtagcag
     3781 tagataatgt ttctaataaa acaaatgttg tgacaattgg ggttgatcct aacttgccac
     3841 aagttattgt tcctaaaaca ggttctacac atttagtaac gattttagcg gtcagcttac
     3901 tattacttgt gttagcaact tttggctacg cagttctgaa gttcaattaa acaaaaaaac
     3961 aaggctgaaa atcagccttg tttttctatt tgaaaatggg attggcacta ataaatacgt
     4021 tgtctgaaga aagtgatccg tctgcagaaa cttcataagc atgggataaa ccaacgatac
     4081 tttgattgtt gtaaagcaag tttgcgttat gagcagcgct atctttccac ataaccatag
     4141 catttactgc tcctgtatct gaaaggatct cgtcaacgta aaggtagaaa ctattgatat
     4201 tttctccttt agcaccgtag cagcctaagc tttctaagag atcttggaaa gtaccgccgc
     4261 caggtcggta atgactgaag cttacttgta attcttttgc acgaatatct gcagcttgtt
     4321 gaacgatatc attgaattgc attggggcaa cgccattttg ttgacggtag ctatttaacc
     4381 aagcaagttc tttttgacgc acatagttgt ttaaatcact tagtgagata tttttgtatt
     4441 tgaaagtgat cgttcgattt tggtcaacat tattgattac acgttgtcca tttttatcaa
     4501 cggtaacaga tgaatcgtca tttctacaca agaagtagtt ttgatcggtc atatttttcc
     4561 aagaagtaga gaaactatct ccctcgtaaa ccgtttgtgt ttcagtagat aaaacagcgt
     4621 ttgtgtcaac gtttatatgg ttcacagtga cgttgtattg atgagtatag gcaaaattga
     4681 ttccttgatt acttgtaacg ttgtttaaag tcacagtgtt gctagagctt tctctaggat
     4741 caagaacaaa attagtgaac ataggtgctt caattgtgat gctttgtcca taaacgacgg
     4801 gataactctt actgtaatta tagagttctt ggatatttgg atcagtatcc gagactttat
     4861 ttggacgagc tttcacttca actagaaaga cttcatcata gtaaaaagta aatgtggtat
     4921 ttgtgttaat cgtttgtgtt tgtgtttcat tcccatttac ttgatactgt ggtgcttcaa
     4981 agttagtcaa gtcggttcta gcttttgcag tgtaacttgt accagctttt actgagacat
     5041 gttcttcttt tagcagcgtt ccagtttgtc gattcatatg acggacagtt accgcatatt
     5101 ctgaaggttt aggtttgtgt tgtaaccctt taaatgcaga atcaagacta tttttagcat
     5161 tatttacttg ggtttgagaa gctttagaat cgtctagtac ttttttagca ttggataaag
     5221 cagtctgaaa attattccaa gatccttctg taaaatctcc tttagcagtt gattttactt
     5281 ggttgtacaa ggcttgtaat tccgttttgt tcacagtagg agctggtttt ttctttaatc
     5341 ccttgtaagc agaatctaat gtgtttttgg cactatccac ttgtttttga gtcgcttttg
     5401 agtcatttaa cactttttta gcattgttta aagcggtttg gaatgcgttc caggtattgt
     5461 cggtatagtc acctttttta gttcccttaa cgctcgtata aagagcttct aaagctgatt
     5521 tattcacttt gggtttagag gtgtcagcac cataccaacc aataccttcg tatttccagc
     5581 catagtcttt ggttaaccat tgtacttctt tccaacttgc agtgtagtga tgcgaaccag
     5641 aggtaacacc tggaaggaaa aggcggtaga tcggtttatt atttttttcg gcagaacgcc
     5701 aggcaggccc ttcataagtc caaccgtaat ttttggtcaa ccatttcact tcatcccaac
     5761 tagctgtgta atggtgatca cgaaggcctt tattgtataa acgatagaca aggtggccac
     5821 cgctagtcgg tgcttcccaa gcggggcctt cataattacc ccacttaatt ttcactaggt
     5881 gatcacgttc ttttgtgcta gaggtgtaaa aatgttcatg gttatttgga ttataaaggc
     5941 gatacatgac gttagttgaa gcagcgtcag cagatccagc aagagcaact gtggcagcaa
     6001 atgttccaat agctataaca gtttttgggt ttaataattt catgttttca actccctatt
     6061 aaatgattaa atattttgtt tacattcata atataccacg gtataccgag gtagtcaacg
     6121 gtataccgtg gaaattttga aataaaaaag ccgccctatg aagagtgtaa ggatggctgt
     6181 ttatatgcta tgaagaaatg gattcggcgt atttggttag tttggcagca ttatcaaatc
     6241 gcattttttc aatcgcactt tttttcttta ttaaatccgc aattgttgat tctgcgactg
     6301 aagaatgttt gctaatttca tatgctgtct tgccagtttc aaatagtaac cattcgattt
     6361 tctctttatc agcgtacatt gcaatgctcc ctcttgattt tttctttatt atagctcttt
     6421 tttgtaataa atcacaatat caagaggaaa agtttctata gaaaggtggg aaattatgag
     6481 tatccgttcc ccgtgtatca aaatgaggtt tgttgctact ttcgttattt aatatactaa
     6541 aataaaacaa cgaaagaagg aatagaatga aaaagttaat aatggtattt acttcgttga
     6601 ttgccatgat tgttatttta ggtggatgtg gtagtgaaaa aaataagcaa gatagtcaag
     6661 ataatggcgt gttaaaacca ggaacagtgt ggaaagaaga agtaggcgga cttgttcata
     6721 atttaaaaat tatagatgat acaacatggg aatattccga aagtgtttgg catcctgaac
     6781 aagtacaaat aacagttgag aagctaaaag actataaagg attagaaaga tataaggtaa
     6841 tcgattcagg tggagtagat ttattcatag gtaaaacagc cttaatagtt ccctataaca
     6901 aagacggttt agaaacgttt aaatttattc caacaaacga aaaagaacat cgagataaaa
     6961 gtgaaatatt aaaagaatac agtactaaat ctggatacaa gttacaaaag actagcgagt
     7021 gattgctagt ctttttattt atcctgaagg aggaatcgca gtggcaaaag ctaaaacgcc
     7081 atttgaaaaa gccgcgtcta aaatcttaga acgccgtggt gtttctgaaa tacagtggaa
     7141 aaaagaagtt cttgaaaaag cacaatttga cctattacga ggaaaagata aggacttaga
     7201 agaatatatc tatcaacgtg aacgtgaaaa acttgtattc gatgaaatta ttaaacatta
     7261 aaaggagcga ataatcttat gtttgaatca attacaatga attttttaac tcaaccgaaa
     7321 gtagtagctg cagttgatat ttttagagca ttacaaactg ccgcaactag tttgaaaaat
     7381 aatctgatga tttttgctgc tgttgcggca gtggcttcca ttgctcttgg aggtattttc
     7441 ttcttctttg ggcgaagagg agctgaaaac gggaagacac acgtaggtta tgtattgttg
     7501 ggggtaggag ttattttagc tgccgcttct attgtagttt caatgttctc aatgtttgga
     7561 caaaccccac aaggactata aaaaaaacaa gtaggaggag taaaaagtac gtggctactt
     7621 tttactcctt tttttatgaa aggagaatat tcatgttttt tacgatttca ggaaatgcaa
     7681 aattggataa agagttaata agaaaagcaa cgaagaaagt gggaaagcgt acttcctttt
     7741 taccgaaaac aattaaatat tctgtcattg aaacggaaga acagctacat gtgttaggac
     7801 aagtgttaga acaagagaat caatcttctg ttaaattgac tctcacgtta catgataaag
     7861 aaggaaaaat agaaaaggct ggcacagaat acgtttttgg cccactggat tatcaaattt
     7921 cagaaggttt ttatcctaca ttgatagatt taatccgcct tgatgtgttt aaagatacgg
     7981 atgaaactat gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa gttttagaag
     8041 aagagttaga gagcgaccaa agaaaagtga atcaagtgag tgaagtcgaa gtaaatgaag
     8101 aagtaccatg ggaacgacca atctttccaa agaaggagtc aaaagaaaaa gagacagtct
     8161 atgttgacga acaagctgat attccgactc agaaatcgat atttgaggaa gagaacgatg
     8221 tgatgggtca attggaggta gatccctttg aagaaacaat ggaggatgaa gagacggacg
     8281 aagtagaagt tgatccagtt gaagctaatg aagagtcaac atggactgaa ggggattttc
     8341 aggaatatat tgaaccactt atcagtgaag aagtagaaaa aaacgaaaca gagatacaaa
     8401 aggcaactgt atttgattat attttggatc aatatccgcc agagcatgaa tggatgaaag
     8461 aaaaaaacaa attattttta aatgagcttt attcgaaata tcaattaccc caaacctatc
     8521 aagcattttt agagagtaaa gaagaattaa tctcacaggg taaaggcaca cttttagaac
     8581 agttagagtc tgtccaatca acaaattggc atgagcttgc acaagaagat ttattagcca
     8641 cctttgaaga acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg acggaacagt
     8701 cgtctaattg ggagaaagta aaagaggaac taaataaaga agaacagcga gtgattgaag
     8761 aagaagtaca aaaagtcaaa gagcggtacg aaaagaaacg cgacaatgct tattccttgt
     8821 gtgtagaaaa gatcaacaat tttactaaga cgaatcaaga agcgttatcg aaagaaaaag
     8881 aaaacttgtt aaaagaacgt attaaagaac gaaaagaaga gttctaccgc ggtttacgta
     8941 cagataagct aaagattagc aaagaattaa atgaacaact gaataagcta tatcaaacaa
     9001 cgactgaagt tcttatggaa aaacacgaac agatccagca agaattacaa gtacaaattc
     9061 caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa gaagaaaaac
     9121 aacggcaaaa agaaaaagag cgtgaacaat tagaaataag aaaacgggaa caggatcttg
     9181 cggaacaaaa aatggcgata gaaaaacaac ggttagagca agaaaaggaa aaagaagcag
     9241 ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg tcaagcacag ttgaattatc
     9301 tagcacaacc tcaaccagta gctgctgttc aaacgccagt gaacaaccaa gcaaatttaa
     9361 ctccttcacc ccagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct
     9421 tgcttctagg tggaggaaca gtatttgctt ttaatcatat caatcaatct tcacaggctg
     9481 attctgtagc cacaacggcg tcaatcaagg aagaagccca ggaacaagca agaaaagagt
     9541 atgaacaaca attagaagaa ttattgaatc gtaccaatca gtcaacaaca gagtcaagca
     9601 tggaaaatag caaagagagt gattctagta aggctaaaga aggatcaagc gattcaactg
     9661 tacaatcaaa cacttccgaa agttcagaat caaatgaggt gaaataatgc gtgttagaga
     9721 aacaacgaag cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt
     9781 ctatcgctat actccctcag gagaagagct accagaaaaa catgacaaac gcatttcgaa
     9841 aaagattttt tatagtggac ttgtatacct ccctttagag tatttagtaa caagagtacc
     9901 agaagttttc aaccgattgg aaattgttaa gggaagctta caaggaataa atctgtttca
     9961 attacaggaa atgccacagg tctttcattt tgattcagct ggtttttgga atccatttac
    10021 tcctacaact agtttgggta cacgtttagc aatttcgaca gtttgtgtgg ttccagctgt
    10081 acttatggga ttgaaaatgg acatgtggtt tcgtcctcta gcagatggac aaaaagggga
    10141 taatcgatta atgaccttaa aagaaatcca acagatttat ccgcaaatac ctgaaacaaa
    10201 aactactttt ccaggttatg gaggtattcc aatcacacat tacaaaaaat attggtacat
    10261 tcaaacagat actgttaata cgtgtattgt tggtacctct cgatcaggga aaggtcagat
    10321 tgaagtgtta gccaccattg acaatttatc tcgtgcagaa aaacaaagtt caatggtcgt
    10381 taatgatcca aaaacagaat tatatgttgg tagtaaagat gaactagaag ctagagggta
    10441 tgatgtatat gcctttaata tcaccgatcc actgcaatca atgagtgata atccattggc
    10501 actcatcgtg aaatactgga aacgaggaga catagatact gcaacacagt taaccaatac
    10561 tttcacaaat acgatttatt atgatgccaa cgcttcagat aacaaatact ttaacgaaaa
    10621 cgcgcagaaa gctgtcaatg cgcttatttt tgcgttgtta gaacaagctg atcaaacagg
    10681 agattactcc aaattgacac cgaataatct cgttgagctt ctttctgaga ttggcggttt
    10741 taattattat ccagatccag acaatgaatt caaacagttg aatgccttag atgaatttat
    10801 gaatcagttg ccacctggaa acattgcaaa aaaacaatat ggggcaacga aaattggatc
    10861 tgataaagcg aagggaaata ttttatcaac ggcaatcacg ggattgaatc catttactct
    10921 aacgaaaatt gcaaagatga cttctcaatc aacgattgat tataaatcgg ttgggtttcc
    10981 aaaatactta gatttgaaat tacatgaaag tcttttaaat caacgagtcg tagtagaatt
    11041 ttatcataag ggaaagaaat ttcatgaaga acgtgtcaaa gtaggctata aaggatttag
    11101 tgaaatcaat tttgattgtc atttagttgc aggtgatcaa gtttgtttaa aatacgatca
    11161 tcaaggaaaa gaatatgtgg cttggtatga attttctcaa agagtattga aagacgaaga
    11221 gggtaatatc gtttatcgga aaaaacgagg agaaacccat ttgccagaat tagaaaaaga
    11281 agccgttcta acattagatg aggaaaaaag taacttatta gtagagtcta ttcggatgca
    11341 ctataccgat aaaccaatgg ctatttttat gcttacacct gattatgatc catctaacca
    11401 tatcattgtt agtatttttc ttagtcagtt atataaagaa ttatctacgc aatgtgttaa
    11461 gactaaagga gataaatgtc accgcaggat tcattttatt ttagatgaat ttgggaatat
    11521 gccaccaatg gataatatgg aaggaatcat gacggtcaca gctggccgaa acatgttgtg
    11581 ggatctgttt attcaatctt atcagcaact ttatgcgaag tatggagaac aaaacgcagc
    11641 gattatcaaa gaaaattgtc agacacatat ttatattatg tcgacagatg ataataccat
    11701 cgaagaattt tctaaaaaag tggggaataa gacggttgaa caagagaaca gtaccatcaa
    11761 ttcattaggt atgaatactc atatcaatcg caatgttgat tcagatcgta tcttaactcc
    11821 tgaaagaatt tctacttttt tagaaggaga aacgattgta ctcgctcctt taaaacgaca
    11881 agatttgaaa ggtcataaaa tacggccgta tcctattttt aatcatgggg aaacgaatct
    11941 tccttatgct tatcaatata ttgaagattt caatccatcg tgggatatca atgatataga
    12001 catacattgt acacatgcgg acttggatct taaggcttta caaattgatt atgattattt
    12061 cctaccagat gaatatgcta gaaatctcta tcatggactg catgatcaat tagaagaaaa
    12121 aaacgaagaa aatatgtttt ataaaatcgt tcaatctctt gagaatgaga cggctaaaaa
    12181 attgttaatt caattttacg aagaacaagc agcagatcga cttcaagaat atgttgaaaa
    12241 gttgatcaaa aataaagaaa tttctgaaat gattgggaaa cggttgttat tggaatctga
    12301 aaaggtggtg aaagaatgat cattggagga attttttctg gcatagccga ctggtggtca
    12361 ggcttattcg gagaagggga tgcaaacccc aaaaccgtaa cgaaagtctt acaagattat
    12421 gggaattatc tgcagtatca agatgtattt agttggattt ggaacacttt tctgtgggga
    12481 ttgattaaag gattatataa tctcaatact ttactagaaa aaaccattta tcaaagcttt
    12541 gatttaaaag acttattgaa cgccgctggg gtaaatgcac tatttcaaag tttgattagt
    12601 aaggtattgg ctcttttttg tgtcgtcaca ttaatttatt taggcttgaa attttttatg
    12661 agtaaacacc caccaaaagt caaaaatatc ttggttaatt tatttatggc catgatcttg
    12721 atttttggtg gtagttcttt aatcgatgaa gggttaaata tcagtaaaag cttttacgga
    12781 gacgtcactg cagcaaataa agcagataaa acgggatcac ctgcttttca aattattcaa
    12841 aataatgtga tcgatgtcag tacgttacta gcaactgacc cgaataaagt agaatcccta
    12901 ccaacagata aaagaaatgc tttaacagcg aaaaatttta aaacagcgaa tatcaatggg
    12961 attattactc ctgaacaagc taaaggtatg gcaggtaatg ataaaagtgg gttatcagat
    13021 atacaaaagg atcggttaaa ggcattacaa taccgattgg aactagatga taatgggaaa
    13081 gaaattcctg ttcaaattag tgacgaaggc ttagcaaaat atgtctatac ttcagggtat
    13141 cgtcgataca gtagtaaacc aggtattatt ttagctggtg aatcttcttt ggcagtagcc
    13201 tatttattta tcctatttac gatcgttact tgcattattg agctggtatt taaaaaattc
    13261 tatttagtga ttgccgcgtc aacggacttt gagacaggtc aacggatgaa aagtgcgata
    13321 gaagatattt ctcaatcctt cttgttattg gcgtttacga ttttagaatt acgaatctat
    13381 accttaatgc tttcaggtat cggtgatttg catgcggcag gaaagatcaa tggattcttg
    13441 tatgtcgttg ctttgattgt actaaccatt gcgctattca aaggctctca atcagttacg
    13501 aaaattttcg gcgttgatac aagcctgaaa aacggcagta attctcttct tagcatgttt
    13561 gccttaggaa atattgcgaa aaatgtgggt agcggagcta aaaaccttgc aagtgctggt
    13621 aagagtggtt taagccattt aaatgatatt cgtaaaaatg gatttggcca tcgaaaagat
    13681 gttacccctg ataacgggga agaacaatca gacgataaag ctacaactgt acctaatagt
    13741 aagaaaacag tttccgaaat gttcgggaaa gcgaaaaatg gtgtttctaa agccgcagaa
    13801 ggtcttggtt acgtcaatga acgtgggtta aaaggaacag ctgaagatct tacagaaaaa
    13861 gccaaagaca ccttagaaga taaattagac ggcacgagag ctaaagaatt ggctgaagct
    13921 gtgaagaatc catccgaagc actagaaaaa caaaaagaaa aagcttcaga agtcaaagaa
    13981 ggactaaaag atgcgtatgg tgaaggacaa gtcaatgcag cagtgaaagc taatacaaca
    14041 accgaaaaag aaaatcaaga aggtatagtg attccttcag tcaaagatca ccaagaagcg
    14101 ttgcactcta atgaaaattc gattgaagag aaaaattcca tagaagaaag aaattcagaa
    14161 gaaggaaaaa atggacagtt accaaaaact gatccagaac aatatcgcta taaacctaac
    14221 agtgaccttg aaaatgagaa gcgaatttca gatttatcaa aagtaaatca agaagacaaa
    14281 gcagaaaatg agggacagtt accaaaaact gatccagaac aatatcggta tagatctgtt
    14341 aactccgaaa atgaaaaacg catttcagat ttaccaaaac gaaatcaaga agtacaaacg
    14401 gatgaggatt ccatcgttct gccaaatgat aatggaatcg aagttggaac tgaaaaatca
    14461 acgaaaattg gtttacctaa ggcacaaaat ttaccaatga aagatagtcg tttgaatcaa
    14521 ttcaaggata atgatcaaag ttttgttcat caatcacctg caggtactgt tcaaatgaaa
    14581 ccaagtcgtt tgcctgaaaa tggaaatctg acaactgttt cttacaattt atctgaaagt
    14641 ggtgagacag ggaatgcgtc cgttcaaaat gttgtgacaa gagaagctgc accaaaacct
    14701 attcaaaacg ttcatacagg ttacaagcca gcaaatgtgg catttactgc accaacaaaa
    14761 ataaatgtga caagagaaac aaaaaacagg aacaatgcat tgaaagaaca actattgagg
    14821 gatatcaaac aggatatcaa accataaaag gcctacaagt taggcctttt ttctatgtat
    14881 gaaaaggaga aatcatttat ggataaatat agtaacatga aaaaccctcc aagatttagt
    14941 ggagaatgga agtttttcgc ttttttcttt ttagatctat taacgcttgg tggctgcttt
    15001 ttttgggcga aaatggtcaa taatcttgtc cacttaccac tcttttttgc actcttgaat
    15061 tatggcttat ggattgcgat agcgttagtg attgtttggc gaccctataa taacccaggt
    15121 caacgacaaa tttttgcgat tatcgagtct attttttttg cggacaagaa ccattatcat
    15181 gcgttagatc ctaaccgaaa ttatcatggt acgtataagg aggatgaata aatgcgagtc
    15241 aaaggacaaa attcagttga aaggaaatcc attttgcagt ttggaaaacc agaagagcgc
    15301 tctatcttgg acttatttcc atataaatcc tgtacagaag agggagtctt aattactaga
    15361 gaagatcatt ttcaacgtta ttatcgtgta gtttctacag acgtggaagg attaaatgaa
    15421 caagagaaac tagaacgaat gaatcagttg accaccgtca tgcgtacgta tgtgccaaca
    15481 attaagttag ttagtttgac cacagagacg gatctaacgg aacaaatcgt gcaaaagcgg
    15541 caattattag ataaaaatcg tatggaacag tccttaggaa gaaatctaca acgattaaaa
    15601 aaatatgaaa agaaattagt cgaagaaatc caggaattga aacgagcaga acaagaacgt
    15661 cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa tcaaggattt acaaaccaga
    15721 aaccgtcagt taatgcgatc tagtggcatt ttagggttaa aaccactcta caaaaaagaa
    15781 ttgatcaaga ttctgtatcg aatgaacaat atgaatgatg agtaggaatg gagaagagag
    15841 aatgaaatat ccgttgatat ggttagaaaa agatgttccg aaggaagaga ccactagaaa
    15901 atttcctcac actttaagcc tgggtaagtt gggaaaaggg caatctttta accaagtaaa
    15961 gaatcaaaca agaagaggtg actgtacatg ttagaagcaa ttaaaacagc tttttcacca
    16021 caaaatgatc aaaaagtact tgatccatta aagcaactag gaaatcggga aatagaaatt
    16081 gtaaaatcca aagggtatga tttagatttt ttaaaacaag tgatgccaca aggtgggtta
    16141 aattttgatg aagaatatgt gacgtatggt aatggttatt cacgatgtgt gacagtttat
    16201 cactacacaa aaaatcctac gcttttttgg ttggccaatc tcatgaacaa tcctcataca
    16261 gtggccacac tcgatgttag cacagatgaa aagcaaaaag tgattgatca aatcaatcgt
    16321 gccttagatg aattaaatga tcgatcagat aatgaacgaa aagcgactga ccgaaataaa
    16381 agcgcggaag aatacctgga actagcggaa tatgctagaa aattgacttc taatggggaa
    16441 atttctaagc aagtcaaagt gagagtacat gtttacggaa acacgattca agaagtagat
    16501 gaacgagcgc aggaactgat cgaaaatctc aaagggatgg attatcgtgc ggtgacttat
    16561 ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca tgttcgatga acaagctgaa
    16621 tggttcggaa atatcaaagg gcaatcacta ccatcgttaa acattggcgg aggtttcccg
    16681 ttccaccacc aaacgctaaa agatccagga gggacacaat atggggaaac catgacaggc
    16741 ggcccatttg tctggaatgc gacgtataaa aatagtgtac ggttgtctac taataccttg
    16801 cttctaggaa tgatgggagc tggtaaatcg accgttctta aaatgatcgc ggaagcccat
    16861 ttagctgcag gagactttat ttggggcttt gaaaagggaa aagactttat tcctttctta
    16921 aaggaatata acggtattat ggtgcgttta gacggatctg acggaatgat caatccttta
    16981 gagatatttg ccacacggac gtatgatgaa gcatccagtt tatatgacga tggatcagtc
    17041 aaagatttga agatcaatga agcagcgtcc tatcaaacac acttagacaa agtggtctat
    17101 caggtacagt tagtgagtcc tcagctaaaa ggaacgatga aagcagaatt taagacctac
    17161 ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg gctttacttc ctcaaattcg
    17221 cgttcaaata ctgatacgca agtcacaggg aaagatccag aagcttatcc gacgttcaaa
    17281 gaatttttag actttttagg acaactggaa ttaccaggtg cttcccaaga gaaaaagaat
    17341 cgaaaagaag aaatggaatc catcgtagaa tccttatgtg agacgtatgg catgattttt
    17401 gatggtcata gtacgattcg acaccttgac caacaacagt tggtcttttt tgacattgac
    17461 tctattacag gcttagatcg aggggtacaa caatgtcaaa tgtacatggc tatgacgttg
    17521 atttggaatc aggcattgat tcaaggaaga agacaacgac gtttaattaa agaaaaacaa
    17581 atggaagaac aagacttcaa acgcttcttg gcgatgattg atgagtgcca taatggtgcg
    17641 acctgttctg cagtgaaaag ctgtgggttg caagtagtct aaaaactatt gtaataactc
    17701 ttataacgag aagtcaaatg agtgggtaac gccacaaagg gcttccactg atactccgac
    17761 atgcaacaag tagtttatgc agaccaacgt tgtgtgaagc tcggtgaagt cggctgaagg
    17821 ataccgtaag gtaaaagaaa tagtgaatac cacgaaagtt ctctagaggt aaagggtgta
    17881 tccgataagt cgggtgtcca aaaaaactta atatggtgag tatgtacaaa cgtactgacg
    17941 aactagcgaa tgtactgtcc gaaaggcata gctcctagaa atgggagaga cagtgagcaa
    18001 aaaatcactc attgtgctaa cttgtggttg agtaaaagtc gttgttatga aagaccatgc
    18061 tgcgttacag gcgtaggaga agttaggggg caaaagctag catctaagtt aaggcatgtg
    18121 gatagatata agggaacagg ggaagccatc aacatggaga gtgcatactc gatgaagtgg
    18181 tagtaagaaa tatgtgaatg acaaataact tactttagtg atggtgaaag tagagtcacg
    18241 actgatgaag gttctgtaat ggaactggag gaacaggctc gattcgtctg taaaagtcat
    18301 gaaacccgtt caaccatggg attctcgtat gactaaaaag gtcacgagcc aattggagcg
    18361 tgataaacca tgacaaaaga aacaagtcca aaagatagga agttgcggca tgcagaatat
    18421 tatggcatga cggaaatctt cgataagata tactccaaaa gtttgaaggg agataatttc
    18481 aagaatctaa tgcagattat catttctgat aacaacattg tcctagcata tcgtaatatc
    18541 aagagaaatc acggtagtgt gacaagagga gaagatggag taacgattaa agatattgaa
    18601 aatctcaacc gtaatgaatt cgtagaaatt gttaggaagc gattcgcaca ttataaccct
    18661 cggaaagtaa ggcgtgtaga aattcctaaa tataatggta aattaagacc gttgggtatt
    18721 ccatcaatat gggatagaat cgcgcagcaa tgcatcttac aagtattaga accaatatgt
    18781 gaagcaaaat tcaataatca cagctatggt ttccgcccaa atagatcagc agaacacgca
    18841 atagcagatt gtatggcccg aatgaatcaa agtcacatgg aatatgtggt agatgtagat
    18901 atcaaaggtt tcttcgacga agtcaatcat actaaactca tgagacaaat ctggtctatg
    18961 ggtatacgag acaaacagct tctcttaatc attaggaaaa tgttaagagc acccgtagta
    19021 cttccaaatg ggaagttgat atacccaaca aaaggaacac cacaaggcgg aatacttagc
    19081 ccattacttg cgaacatcaa tctcaacgaa tttgactggt ggatagcaaa tcagtgggag
    19141 gaaaaagatg ccaaagagct aaaacatcat tttaggaaaa atggagcttg ggataaaggt
    19201 aatatctatc acaagctcag aaagagtaca actctaaaag aaatatacct agtgagatac
    19261 gcagatgatt ttaaaatttt ctgtagaaat tataatgaag cgaaaaaagt tttctatgca
    19321 actaaaatgt ggttacagga gcgtctaaag ttaccaatat ctgaagaaaa gtcaaaaatc
    19381 acaaatcttt caaaagaaga aagtgaattt ttaggcttta cgatgaaatt agtgaccaaa
    19441 ggtaacaaaa aggttgttaa tacgcatata ggtacaaaag cacaaagcat gattaaagtg
    19501 aaactaaaaa aacaaatcaa acgaattcaa aaaagtccta atagcaaaga tacctttatc
    19561 tatattggag tctataacag tatggttatt gggattcata attacttcaa aattgcgact
    19621 cattgtagtg tagcaatgcg aaaaattgca agagaactca gaccaagttt tgaaatacga
    19681 ctacgagaac aaggaatctc aaaagaagga caatacggaa agtacgaagg atacaagcct
    19741 tacctacggt caggacaaat acgatatata agagggtttc caatattacc agtagggtat
    19801 attcaacaca gacatccgat gtgtaaaaaa agaaatatta ataaatacac cgaagaaggt
    19861 agggaagcca ttcataagaa gcaacaaacc gtagaagaat ggaagatatc gtggttgtgt
    19921 agcaatccag tgcttagtaa gagagcaacg attgaatttg cggacaatcg aatttcaaaa
    19981 ttcatcgctc aaaagggaag atgtgcagta acaggtgagg agcttatctt atctgaaatg
    20041 cattgtcatc atattatccc ttatcatgaa agcaaaagtg atagctatga aaacctagtg
    20101 attgttactg aggaggttca tcgggtaata cacgctacac aaagtgaaac gattgaggaa
    20161 cttctaaaat atctaaaatt aaatccaaaa caaaaagaaa aactcaatga gttgagatta
    20221 aaagttggta acgaagaaat atcttaataa atatttgtag gacgggaaaa cagaatgtct
    20281 tttatagacg agggaacgcc gtatgcggtg aaagtcgcac gtacggtgtg gactggggga
    20341 aaagacggag ataatatcaa actcttacct atcagtatcg attaatccag attttattga
    20401 aacggtaaat tatgtcacaa actttcaacg agaaatgcgt aaagtagaag caatgactgt
    20461 tttagctaca caatccccac aagaaatggt gccagaaaat atgtcttctg ataacttctc
    20521 gaaactgaag aaagtctttg aattttcttc tacgaagatc tttatgcgta tggatgaatc
    20581 tattttacgt catatagaag gattagtggg taaatcaatg acgaactcag aattagaatc
    20641 cattcctcaa caaaatcaag gagacgctgt gatcaatttt ggctcaaaag aaaccattcg
    20701 tgttcatttt agtccgaaca aacgtcaatt aaaattgttt gatggaggaa aataatggaa
    20761 aaccaaaatg aaagtttaat caagcagtat gttaagcgtc gtgcaaagcg gcgcttattt
    20821 ttgtggttgt ttggtacttc tgcaggtttg attactattt tgatcactgt gttcgtgacc
    20881 ctatttctaa tacttgcggc gggctcgatt gataattcgg attcagattc aagcagtggt
    20941 ggcgaagctt ttacaggaga atattcggaa ggcttaccga tttataaaga aatcaaagga
    21001 agaggccctt tttctgatga gattgcgcaa tatgccgtag gtgcagcagt gaaatataaa
    21061 ttattaccaa gtgtgatcct ttcgcaatac ggttacgaat cagcgtttgg tacatcggcg
    21121 tccgcaagaa atgatctcaa ctattttggg attacttggt ttgatggttg tctgtttcct
    21181 aaaggaactg cacgaggaat tggtggcatt gaaggtggct ggtacatgaa gtttcccaac
    21241 tcaaaagctg cattttccta ttacggcttt atggtagcca cacaatccaa ttttaatgct
    21301 tgtgtaggaa ataaaagccc tggcgcaagc ttattgatct taggacgagg cggttatgcc
    21361 gctgcaggaa ttacggaaga tagtccgtat tatacaggtt gtatgtcaat tatcacttct
    21421 aataaattaa ccgaatatga tgaattcgcc atcaaacatt ggggtgaagg tggcaataac
    21481 aacggcacga ttactggtga atggacgaat ccattccctg gcagcagttt agataaaagt
    21541 tccttcagcg gtggtcaatt atttggaacg aatccaggcg gtgaattccg tccgaacggc
    21601 ttccatgatg gattagattt tggttcagtt gatcatccag gaagtgagat tcatgcggtt
    21661 catggcggaa aagtcgtgta cgttggcaa
//
LOCUS       DAFGZE010000049        20935 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.051, whole genome shotgun sequence.
ACCESSION   DAFGZE010000049 DAFGZE010000000
VERSION     DAFGZE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.051"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            612..1301
                     /locus_tag="LTU54_001916"
     CDS             612..1301
                     /locus_tag="LTU54_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8296626.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1288..2496
                     /locus_tag="LTU54_001917"
     CDS             1288..2496
                     /locus_tag="LTU54_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8296627.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2790..4061
                     /gene="serS"
                     /locus_tag="LTU54_001918"
     CDS             2790..4061
                     /gene="serS"
                     /locus_tag="LTU54_001918"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBL8296628.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4610..6094)
                     /gene="guaB"
                     /locus_tag="LTU54_001919"
     CDS             complement(4610..6094)
                     /gene="guaB"
                     /locus_tag="LTU54_001919"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBL8296629.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6599..7294)
                     /locus_tag="LTU54_001920"
     CDS             complement(6599..7294)
                     /locus_tag="LTU54_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HBL8296630.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7384..8484)
                     /gene="ychF"
                     /locus_tag="LTU54_001921"
     CDS             complement(7384..8484)
                     /gene="ychF"
                     /locus_tag="LTU54_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBL8296631.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8493..8684)
                     /locus_tag="LTU54_001922"
     CDS             complement(8493..8684)
                     /locus_tag="LTU54_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBL8296632.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8705..9595)
                     /locus_tag="LTU54_001923"
     CDS             complement(8705..9595)
                     /locus_tag="LTU54_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBL8296633.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9582..10349)
                     /locus_tag="LTU54_001924"
     CDS             complement(9582..10349)
                     /locus_tag="LTU54_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL8296634.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10361..11074)
                     /gene="rsmG"
                     /locus_tag="LTU54_001925"
     CDS             complement(10361..11074)
                     /gene="rsmG"
                     /locus_tag="LTU54_001925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBL8296635.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11306..13207)
                     /gene="mnmG"
                     /locus_tag="LTU54_001926"
     CDS             complement(11306..13207)
                     /gene="mnmG"
                     /locus_tag="LTU54_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBL8296636.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13224..14621)
                     /gene="mnmE"
                     /locus_tag="LTU54_001927"
     CDS             complement(13224..14621)
                     /gene="mnmE"
                     /locus_tag="LTU54_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBL8296637.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14920..15303
                     /locus_tag="LTU54_001928"
     CDS             14920..15303
                     /locus_tag="LTU54_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296638.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15471..16229)
                     /locus_tag="LTU54_001929"
     CDS             complement(15471..16229)
                     /locus_tag="LTU54_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HBL8296639.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16247..17065)
                     /locus_tag="LTU54_001930"
     CDS             complement(16247..17065)
                     /locus_tag="LTU54_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL8296640.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17062..17418)
                     /gene="rnpA"
                     /locus_tag="LTU54_001931"
     CDS             complement(17062..17418)
                     /gene="rnpA"
                     /locus_tag="LTU54_001931"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBL8296641.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17536..17670)
                     /gene="rpmH"
                     /locus_tag="LTU54_001932"
     CDS             complement(17536..17670)
                     /gene="rpmH"
                     /locus_tag="LTU54_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBL8296642.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18193..19527
                     /gene="dnaA"
                     /locus_tag="LTU54_001933"
     CDS             18193..19527
                     /gene="dnaA"
                     /locus_tag="LTU54_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBL8296643.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19725..20855
                     /gene="dnaN"
                     /locus_tag="LTU54_001934"
     CDS             19725..20855
                     /gene="dnaN"
                     /locus_tag="LTU54_001934"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBL8296644.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
BASE COUNT         5989 a         4263 c         3836 g         6847 t
ORIGIN      
        1 tttcaaaggt ctacttcatc aggagtgttt cccgattcgt ttttggctgc cgtagcaact
       61 tttatatcat atcagagaga caagtaattg tcaactcttt tttaaaagtt ttttcgattt
      121 cgtttttcga aatcttccag caactcattt atcataactt atcgtttgtt atttgtcaag
      181 aacttttttg aagttttttt cgatagaatc aagcttcgca agtggttctt ttttaacgac
      241 tcagttatct tatcatgtca tttccaacat gtcaacaact tattttttat tatttaataa
      301 gttattggtg ttcttttgaa caaataataa tatatcaaca tcacatgtga aagtcaataa
      361 gaaaatgctt atttatcaac atttctagct tacatgatca ttggttagtt ctggtccttt
      421 ttcgttcgtc tttataactc cgagagacgt aaaatccttt tgttttcagt ttaatgttca
      481 tgttcttgtc ttattcacac caactagatg tctcaccttc taaaattttc cttaacaaag
      541 tgctgctttt atctatttct gctttttttt gctagaataa tagtaacaat atgactctgg
      601 aggatttatt tatgaaaatt ttgattgctg acgatgataa agaaatcgtt gaattattaa
      661 gtatctatgt tcacaatgag gggtatgaag cagtaaaggc ttatgatggg aaagaagcat
      721 tatctaaaat cagaacaatc cctgatattg aattacttat tttagatatc atgatgcctg
      781 agatggatgg gatgcaagta gtgaaagagc tacgaaaaga atcccaaata ccaatcatta
      841 tgctgacagc aaaaactact gatatggata agatcaaagg actggtcgct ggtgcagatg
      901 attatgtgac caagccattt aatccgttag aagtaatggc tagagtaaag tctttattac
      961 ggcgtacaaa aatgcagatt gccccagatc agcctgatgt attagaagta aatgcattaa
     1021 tcatcaataa agactctcat gaagttaaaa caatcgatgg gaaagaaatc cagttaacag
     1081 ctttggaatt cggtatcctc tacttgcttg ctagtcatcc taatcgagta tttagcgcgg
     1141 atgaaatttt tgagcgtgtt tggaaacaag aaagtattgt ctctgctaaa actgtcatgg
     1201 ttcacgttag tcatttaaga gataagattg aagaagcaac tggtggtgaa aaagtcattc
     1261 aaaccgtttg gggagtgggc tataaaattg acgcaagata gtaaacgagc aactgaaaaa
     1321 cgccgtcgga tcaatttgac ttcaaaagaa atcagcgaat tgttagcgga aggaatcatc
     1381 acgattatcc ttttgcttct attaaatgtt tctattctgg ttgttatcag ttcggtgatc
     1441 aacagttcac cttctttgac gaatgcgatt tgggattcaa agaatatttt tgcagaacga
     1501 ttgaacactg atttgttctg gaacggccgg aattttatta ttcccttttt ctttctatta
     1561 gatattggtg ttttgtattg gcgcttgatt cgaagatatc gccaaatgca attgcggcat
     1621 atcatcagcg aacttcacta catcgcaaac ggaaattatg atcatcgtat cccttttgaa
     1681 ctgagtggtg acttaagccg agtggtaaca agtatcaatg gattggtgga tagtaccgta
     1741 gcagcgattg aagacgaacg taaaatcgaa aaatcaaaag atgaattgat cacgaatgtc
     1801 agtcatgata tccgtacccc actcacgtcc atcattggat acctaggact gatagaagat
     1861 ggccagtatc actcagaaga agatctttta aagtatacgc atactgctta tatcaaagca
     1921 aagcagatga aatctttagt ggacgactta tttgaataca ctaaggtacg ccagcctgct
     1981 gtcccggtga atttttctgc ctttgacatg atccagttga tcgaacagtt agcagctgat
     2041 tttgagttgg aagcttctaa aaaaaatatc caaatacttg ttcaatcaaa agtagactcc
     2101 cttatcatgg atggcgatac cgaaaaactt gtacgtgtct tcaataatct tttgacgaat
     2161 gcgttgaaat acggaaaagg agcaacgaag atcgtaatcg aagtagaacg tatcggttca
     2221 gaagttgttg caacagtcaa aaataacgga gcaatgatcc cacaacaggc aatcgataat
     2281 ctattcgacc gtttttatcg tgtagaagaa tctcgttcac aagcaactgg aggaactgga
     2341 cttggtttag caatcgctca aagcattgtg gctttgcacg gcggatacat ttatgcaaaa
     2401 tcagataagc aatggacttc tttcatcatg caccttccaa tcaagaaaaa tgaaaaatta
     2461 cctattgata cacaggaagt tattgacgaa agctgaaata ttcgctattc ttattacaat
     2521 tattagaata aatgaaaaac acagaaccca gtacacttta ttctttttca gagagctgat
     2581 ggcaggtgta aatcagcaaa ggataatttg gaagttccag ttcgtttacg tgccctttcg
     2641 aaataagaaa ggtcggttca acagccgtta tgtgttcaac aagtgcagct gacgctgaac
     2701 ttgggtggta ccgcgaattt cagactgatt cgtcccaaga ataatgggat ggatcagtct
     2761 ttttttattt tcaaaaagga ggaaatcata tgttagatgt aaaaatgatg cgacaaaata
     2821 ttgaagaagt acaagaaaaa ttgatgactc ggggcgtaaa aaaagaaaca ttagctgaat
     2881 tcatggaact agatgaaaat cgtcgtaagc tactagtcaa aagtgaagaa ttaaaaaaat
     2941 atcgaaatga tgtttctgct gagatcgccc aattgaagcg tgcaaaagaa gatgcttctg
     3001 ctaaaatcgc tgaaatgaaa gaagtcggtg gcaacatcaa agcattggat actgaaattg
     3061 ctgaagtgga tgaaaaattg aaacaaattg caactacttt gccaaacctt ccccatgatt
     3121 ctgtgccagt tggaacagat gaagaggaca atgtagaaat ccgtcgttgg agcgaaccac
     3181 gtacatttgc ttttgaacca aaaccacact gggaaattgc agaaaatctt gatattttag
     3241 attttgaacg tggcgcaaaa gtagccggta gccgttttgt ctattacaaa ggattaggtg
     3301 ctcgattaga acgtgctgtc tacaacttca tgcttgatca gcatatttac gaacacggct
     3361 atactgaaat gatcactcct tatatcgtca acagtaaagc gatgtttggt acgggacaat
     3421 ttccaaaatt caaagaagat gttttccaat tggagaatac tgatctaaca ttaatcccaa
     3481 cagctgaagt accattaact aactattata atgatgaaat tttagatggt gcagatttac
     3541 ctatttattt cactgcacta agccctgctt ttcgttctga agccggaagt gctggtcggg
     3601 atacacgagg actgattcgt cttcaccaat tcaataaagt agaaatggtc aaattttcgg
     3661 atgtagagca ttcttacgaa gaattggaaa aaatgacagc tgatgcagaa gatatcttga
     3721 aaaaattgca tttgccttat cgtgtgatgg ccttatcaac tggagacatg ggcttttctg
     3781 cagctaaaac ttatgactta gaagtatgga tacctgcaca agatacttat cgtgaaatca
     3841 gttcttgttc taactgtgaa gatttccaag cacgccgcgc aatgatccgt tatcgcgata
     3901 atgatggaaa aatccactat actcatactc tgaatggttc aggacttgca gttggccgta
     3961 cagtagctgc cattttggaa aactaccaaa atgaagatgg ttctgtcaca gttccagaag
     4021 cgctcgttcc ttatatgggg aatctgaaag ttattaaata aagaggctgt gaaaaagccg
     4081 cttagtttcg agtattaaga aaaattttgt gaaaatggct ttccacattt ttgcataaat
     4141 tggacttaat accgagaaac tggcttttga acaccattta tcgagaggtt gtgaaatcag
     4201 cgaattaacc tgagtattag agaaaaaaat ggaaaaatag tccctcatat tttaccgttt
     4261 gttgaactaa taccgagggt cagctgattg aacaccgttt agtggagagg tcgtaagcct
     4321 accccccagc tcctagttac aaggacaaat tcaaaaaaca gctcttcata ttttatgaat
     4381 gttgtacttg tgattcggag gtggcaaggg aacactgttt attcaaaaag aggctgggac
     4441 agaagcgttt aactccaaga aataagaaga aattcacgaa aattgctttt caaatttttg
     4501 tgaatttctt cttatttccg aaggagttgc ttctacttcc gccgtttata cgtgtttaga
     4561 gcgtgagaca aaagtgttct ttacttttgt cccacgctct ttaatgtttt taagattcaa
     4621 ctgaatagtt tggtgcttct tttgtgattt gtacatcatg agggtgtgat tcttttaatc
     4681 cactgccgct catttggata aattgcgctt catcacgtaa ttgctgtaga ttggctgccc
     4741 caacataacc cattcctgaa cgcaaaccgc cgatcatttg gaaaataata tctgcaacac
     4801 ttcctttata agcaacccga ccttcgattc cttctggtac taatttattt gcttcattga
     4861 cactaccttg gaagtaacgg tcttttgaac ctttttccat cgcgcctaat gagcccatac
     4921 ctcggtatgt tttgaatcga cgtccttgga agatttcgaa ttcacctggt gattcatctg
     4981 tccctgccaa catacttcct agcatcactg catgtccgcc ggcagctaat gctttgacga
     5041 tgtcaccaga atatttgatc cctccgtctg caataatcgc tttgccatat tgacgagcaa
     5101 ctgatgctgc atcataaata gctgtgagct gaggtacacc tacaccggct accacacgtg
     5161 tcgtacaaat cgatcctggt ccgataccaa cttttacgac atctacacct gcatcatata
     5221 aagcttttgt cgcttcagcc gtcgcaacgt ttcctgcaat caatgttgca tctgcaaaag
     5281 ttgaacggat ttcttgaatt tttcggatca cacctgcact atgtccatga gctgtatcga
     5341 tgatgattgc atctgcccct gcttctaata aagcattcgc acgctcaaat gtatcgcttg
     5401 ttacaccaac ggctgctgct acaagcaatc gaccatgttc atcttttgct gcatttggaa
     5461 attcgatcac tttctcgatg tctttgatcg tgatcaatcc gcttaaacga ccttcgttat
     5521 ctacgattgg taatttttcg attttatgtt tttgcaaaat cttttctgca tcttttagag
     5581 aggtacctac tggggcagtc actaagttgt cttttgtcat cacatcagca atcggcatgt
     5641 gataatctgt tacaaaacgc atgtcgcggt ttgtaattat ccctaccaat tttctgtttt
     5701 caagtgtttc aacgattggc acaccactga tacgatattt gctcatcaat tcttctgcgt
     5761 ctgctacaag atgctgtggc gttaagaaga acggatcaat gattacgcca ctttcagaac
     5821 gtttgacttt tcttacttca tccgcttgct ggctgatcgt catgttttta tggatcacac
     5881 ctagtccgcc ttgtcttgcc atagcaatgg ccatcttgct gtcggtaaca gtatccatac
     5941 ttgcactgat gattggaata ttcaacttaa tatttttagc taattgtacg ctcatatcca
     6001 cttcgtttgg taacacgtga ctttctgctg gtatcaataa gacatcatca aaagtaaatc
     6061 cttttttcgt gaactttgtt tcccagttgg acatttttag taccactcct ctaatttagt
     6121 ttatctatta aaataattga ttttttcatt taagtcaatg atctacttgg aatcaagtta
     6181 gctttctcat agaataccaa tataaagcgt ttacatctgt gtgtattaaa agagattgta
     6241 aaaaagctgt cctgcatcaa gtaataagaa cagccaatca aaaacagctt ctcatgtttt
     6301 tgaattggtt gggtttatta ccgcagatgc aaacttttga acaccgttta gtggagaggc
     6361 tgtgaaaaag ctgcttagtt tcgagtagta agaaaaattt tgtgaaaatg gctttccaca
     6421 tttttgcata aattggactt aataccgaga aactggcttt tgaacaccgt ttatcgtaaa
     6481 aagggactgt gacaagactt ttgtcacagt cccttactat tatttaacta tggataaaca
     6541 aaatgaggtt ctttactacc cctagatatc tagcaaaata gctgatcttt ttgctagttt
     6601 atcttcttgg gacggataaa cttccaataa tcccgtattt tttcttcaag tagtaacgga
     6661 ttgctaaagc agttgccccg ataatgataa ctactactgg atctaatact ggattgataa
     6721 ctggtggcaa tagtgttgtc gcagaaaaca gtacgaccca aagaagtgtc gttccaccta
     6781 ggataagcaa tgtcttaccc attcctggtt tcttgcttcg atctgctccg ggatattcat
     6841 attgatagat gtatttgtac atcatataaa agacccagcc accgattgca gaacctagaa
     6901 cgagtgtaga aaagccataa gctggcgttg ttccacgtga aaacaatccg gtaatcgcaa
     6961 tcatgatact taatagacca agcaacagca acgtattatc taaccacatc aataaagggg
     7021 tggatgaatt ttttacttct gctggcttat tgatgattgc ttccgttctt tcagagactg
     7081 tcccaaacaa ttgacgggct gttcttccgc ctttttgttc tgcaactaaa gtaggcagaa
     7141 tgtcatgaag ggcttttact ttttcttctt cagacaaatt cgctgcttct aaagattttt
     7201 tcaaatcaaa aatgtactgt tgatttcttt tggttaattt tgtttctaaa ctattgtttt
     7261 cttttacgat tgctcgtaat tgttctggtt ccattatcta atagtcctcc ttagccgttc
     7321 cttttctcaa cggtcgtcac aaaagacatt ctaccatatt ctacagctag tttgatagcc
     7381 cttttacaca ttaaagcgga ataacattac gtcgccgtct tgtacgacat aatcttttcc
     7441 ttctaatcga acacgtccag cttcttttgc tgcatgcatg tttccgtaat gatccaagtc
     7501 ttcaaaagag acagtttctg cacggataaa tccgcgttcg aaatctgaat ggatgattcc
     7561 tgctgcttgt ggtgctttga ttcctttttt gaatgtccaa gcacgtactt cttgttcccc
     7621 agcagtaaag taagtagcta agcctaacaa atcataagca gcacggatca actgatccaa
     7681 acctgattct tcgattccta aagcttctaa aaattccgct ttgtcttctt catctaattc
     7741 tgcgatttct tcttctgcac gagcactgac cacgatcact tctgcatttt cttctgcagc
     7801 aaagttgcgc acttcttgta cataatgatt cgcgtctgca tctgctacgt catcttctga
     7861 gacattggct acataaagaa ttggttttgt cgtcaataaa aatagtgatt taacgatttt
     7921 ttgttcttct tcagtaaatt cgatcgttcg ggcagatttt ccttcttcca gtacaggttt
     7981 gattttatcc agtacagcta gttctgccac tgcatcttta tctttggttt tagcaacttt
     8041 tgctacacga gtatagcgtt tgttgataga ttctaagtct gctaacacta gttccaagtt
     8101 gatcgtatca atatcttcta gtggatcaac acgtccttct acgtgagtaa tattatcatc
     8161 atcaaaacag cgaacaacgt gacaaatagc gtctacttgg cggatatggc ttaagaattg
     8221 atttcccaaa ccttctcctt tacttgcgcc ttttacgatt ccagcaatat ctgtaaattc
     8281 aaaagtcgtc ggaacagttt ttttcggatg tacaagttct gttaagcgtt gcaaacgcca
     8341 gtcaggtact tctaccatcc ctacgttggg gtcgatcgtc gcaaagggat agtttgctgc
     8401 ttctgctcct gcttttgtga tcgcattaaa caaagttgat tttcctacgt tgggcaaccc
     8461 aacaattcca gcagtcaatg ccataaattt attcactctt tctctgcatt tttttcaatt
     8521 acttttttta atttcttttc aaaatctcga cgggtcatca tgacgatatg tccgcaattc
     8581 atacatctga ttttgatatc tgcgcccatt cgaatgactt cccagcgatt cgtctggcaa
     8641 gcgtgaggtt ttttcatttc aacgatatcg tgcaagtcgt acataaaaat agcctccttt
     8701 ttgtttattc ttcatcaaat tgaatattca agatatcaag aattcgagtc aaatcttttt
     8761 gagaaagata ttcaatctcg atcttacctt tcccattttt ttcttggata gctacacttg
     8821 taccaaattt atccatcaaa cgatcttcgc tttctcggat ataatatggt ttttcctttg
     8881 ctagccttgg gatttttttc ttttcttttc catcattttg atttaactca gaaacaagct
     8941 gttccagttg gcggacagtt aaattttcag ttactgcacg attggctaat ttcaagatca
     9001 atgttttatt ttttaaacct aacagcgtac gggcttgtcc catagataaa cgctgatctt
     9061 gtaccatttc cttgacaaga tccggtaaag aaagcaagcg caaataattg gcgatataag
     9121 gtctgctctt tcctaaacga ctagctactt ctgcttgtgt tagtttcagg tttttcatca
     9181 gcatctcata tgcttctgct tcttctaacg gattcagatc ttctcgctgc aagttttcca
     9241 gcactgctac ttgcatcatc gcttcttcgt caaagtctct aacgatagca gggatcgttt
     9301 ctttttcagc tagtttggac gctcgaaaac gtcgctcccc tgcaataatc tcatatcctt
     9361 ttactgcgga acggcgtacg atgatgggtt gaaagacacc tgattgttca atagaattag
     9421 ctagttcctg caaagatact tcttcaaatg ttttacgggg ctgatacgga tttggtcgca
     9481 gctcattcaa tggtagctgg acaacggtat catcttttac atccaccgtc tctaagtttt
     9541 ctaaatcttg aaacagtgca tcgattcctc ggcctaatcc tttacttttc ttcacgactt
     9601 accacttcct ttgccaatgc ttggtagact tcagctcctc gagaacgtgg atcgtagtcg
     9661 atgatcggtt ttccatgact aggtgcttct gataaacgaa tatttcttgg aatgatcgtt
     9721 tcgtaaactt tttcgcggaa gtatttacgt acttcttcta cgacttcatt tcccagattt
     9781 gttcgtgcat catacattgt cagtaagact ccctcgatct ctaattctgg attaaagtgt
     9841 ttttggacta aacgaaccgt attcagtaat tggcttaatc cttccaatgc gtaatactca
     9901 cactgaacag gtatcaaaat cgaatcactt gctgtaaatg aattgattgt cagatggcca
     9961 agagaaggcg ggcaatcgat caaaatataa tcatactgac tgcttacttc tgctaaagat
    10021 ccttttaatc ttgattctct tgccatcatt gacgtcaatt cgatctctgc tcctgctaat
    10081 tggagagtcg caggcacaat actcaaattt tcgtgttcag tgatcaaagt tgcctcatca
    10141 attggcaatt cattgactag aacatcatag atgtctcttg tcacatcagg ttttctgatc
    10201 ccgacaccac tggtagcatt tccttgtgca tccatatcca ctagcaatac ttttttgcca
    10261 aggctagcaa gacaagcacc tagattcacc gtcgtcgttg ttttgccgac gccacctttt
    10321 tggttcgcaa cagatattat ttgtgccatc tcttctcctc ctatttgatt ggctgcttat
    10381 taggcagtcc gggctttctt gggtatttct ttggggttgg tttctttttt tgaataacga
    10441 tgatatgccg ttgatcagag acattcggca aggtaaccgc ctcttctttg aggaatttac
    10501 cacctaataa agcaatcgct tgttttgctt ctgtcaattc ctcttcactt tttgctgctt
    10561 tcaatgcata aaaatagcct tcttgtttag ccaagggcaa acacaattcc gctaacacat
    10621 tcaaacgagc aaccgcacga gcagtgacaa ggtcaaatga tgcacgaaag tgaggatttt
    10681 gcccaaatgt ttctgcccgg tcatgatata aggaagcatc gattcctaat aatcgggtta
    10741 actcagataa gaaggttatt cgtttattca gagaatcaac gatcgttatc tgcagttctg
    10801 gaaaggcaat ttttaaaggt atgctaggaa accctgcccc cgaaccaaca tcgcataaag
    10861 ataaaggttg cgtaaagtct acttcaaatg ctaatgtaag tgaatcgtaa aaatgcttca
    10921 gatatacgtc tttttcatcc gtaatagctg taaggttcat cttttcgttc cattcgacta
    10981 atgtccgata ataaatagcg aattgattca tttgatgatc ggtaagctct atcccttttt
    11041 ctgccaagga ttggcgaaat tcttctggta tcataaatat tcctttcatt gtgaaacagc
    11101 aattgtttca cagattctct ccctacttta acgcaaaatc tctagacatg caatccttcc
    11161 atatggattc ttaaaaattt gaaaagtaag aaaagccgat agactttttc tttctagtat
    11221 gactcataaa atatacacag aagagagaac tagctttgag caatttctgc aaaaactagt
    11281 tctctcttct atttattatt acgtattacg ctggttcact gactttcgca atcttccctt
    11341 gttcgatata gaccattagg atacttaggt ctgcagggtt tacgccactg attcggctag
    11401 cttgggcaat tgtttctggt tggattttct gcaatttttg ttttgcttct gttgctaatc
    11461 cgttgattgc tgcgtagtcg atgttttctg gaatcttttt cgcttccatg cgttttaatt
    11521 tttctacttt ttccatggct tttttgatgt agccttcata tttgatttgg atttctactt
    11581 gttcgatttc ttttcgtgtc aattcttcat tttctggaat aaaccgtaga agttctgcat
    11641 aagtaatctc aggacgtctt aaaaagtcgc ttgctagtac accatctttt aatggtgccg
    11701 cattaatcgt tgctaaaaag tcttggactt cttctgttgg tttgattcgt acattcatca
    11761 aacgtttgat ttcttgttca actgcttctt ttttcttcaa ataattcttg tattgatctt
    11821 cttttactaa gcctacacgg tgacccattt ctgttaatcg caaatctgca ttatcatgac
    11881 gtaaaagcag acggtattca gcacgtgaag tcagcaaacg ataaggttcg ttcgttcctt
    11941 ttgttaccaa atcatcgatc atgacaccga tgtaaccgtc acttcgtttg atcactaaag
    12001 gttcttttcc ctgaactttc aaagctgcat tgataccggc aatcagccct tgtccagccg
    12061 cttcttcata accgcttgta ccatttgttt gtccagctgt ataaagattt tcaacgactt
    12121 tagtttctaa tgtcggacgg agctggtgtg gctctactac gtcatattcg atagcatatc
    12181 ctgtacgcat catttctact ttttctaatc cagcaataga atgaagcatg ttgacttgaa
    12241 catcctctgg taaagaagta gataaacctt gaacatagac ttcttccgta tttaagcctt
    12301 ctggttccaa aaataattga tgacgtggtt tgtctgcaaa acgcacgatc ttatcttcga
    12361 tggatggaca atagcgagcg cctacccctt caacgatccc tgtaaacatt ggggcacgat
    12421 gtaagttgtc tcgaataatt tggtgagtcg tctcacctgt ataggtcagc cagcaagatt
    12481 cctgatctag tttataggca ctatctggtg tgctgaagct aaaatgattc ggctctttgt
    12541 caccaggctg ctcttctgta acagaatagt cgatcgtact tgattttact cgaggcggtg
    12601 ttcctgtttt gaaccgttcg atagcaaagc caagttcttt tagatgattc gctagtccaa
    12661 tagaaggttg agagttattc ggacctgatg aatatttcaa ttcaccgata atgatttctc
    12721 cgcgaagtgc tgttccggct gtaataacaa ctgctttggc ccggtagctc gctcctgtgg
    12781 acgtcactac tccatgacaa acaccatctt cgacgatcag ttcttcgacg attccttgtc
    12841 ttagcgtcaa atttggttct ttttcgatcg tatgtttcat ttctgttgaa taagcatgtt
    12901 tgtctgcttg tgctcgcaat gctcggacag ctgggccttt tcctgtgttc aacatacgca
    12961 tttggatata ggttttatcg atattcttac ccatttctcc gcctaacgca tcgatttctc
    13021 gaacgacaac gcctttagct ggtccgccta cagaaggatt acatggcatg aatgctacca
    13081 tgtccaaatt tattgtcagt aataatgttt ttgatcccat acgtgcagca gcaagtgcag
    13141 cttctgaacc ggcgtgtcct gcgccaacaa cgatcacgtc atacgcatct gcttcataat
    13201 gttccaatcg tttgttcctc cttttatttt cctaaacaaa attgactgaa taattgagtg
    13261 attagctcat cttgtacact gtcgccgaca acttctccta gatagtccca gcaacgcgtc
    13321 atatctattt gaacgagatc cacaggcatt cctgaatcaa tacctgcaat gacttcttca
    13381 agtgagaggg aagctttttc taacaacgcg atatggcggg tattagaaac ataagtagcg
    13441 tctctttctc ctgtttctcc gctgaagaat aaatcagaaa tagctgattc caaacgatct
    13501 agtccgtcgt ttttagctac ggaaattgaa aaaactggtt cattttcgat caatttcttc
    13561 aatttttctt gttccagctg tgctggcaaa tctgttttat ttagaagaat gatccgtttc
    13621 aatccacttg tcgcttccaa taattgttca tcttctgctg tcagtggttc gctttggttt
    13681 agaacaagta agatcaagtc tgcttctgct aatgcttttc ggcttctttc gaccccgatc
    13741 cgttccacca catcttctgt ttcccgaatt ccagctgtat cgattagctt gagcggtacc
    13801 ccccgaacat tgacatactc ttcgattaca tctcgagtcg ttcctgcaat atccgtaacg
    13861 attgcttttt cttctcttaa caaatggttt aacaaacttg atttgccgac atttggccgg
    13921 ccgatgattg ccgtacttaa cccttcccgc agaaccttcc cttgcttgga tgtagcaagt
    13981 agcgcttgga ttctttgttg gatcatctgc gctttttcaa gcaataactt cgtagtcaat
    14041 tcttcgacat catcatattc tggataatca atatttactt ctacttgtgc taatgtctct
    14101 aaaatctctt gtctcaatga gcggatcaaa gcagacagat tgccgtctaa ctgattaaga
    14161 gctagtccca ttgctttgtc tgtttttgcc cgaatcaaat ccatcacagc ttctgcctgt
    14221 gaaaggtcaa cacgaccatt caagaatgct cgttttgtga attcgcctgg ctcagctaat
    14281 cttgcacctt ctcttaacaa tagctgcaaa atttgattga ctacaacgat cccaccgtga
    14341 caattgattt ctactacatc ttcccgcgta aatgtctttg gtgctctcat cacggagacc
    14401 atgacttcat cgacaagctg attgctcttg ggatcaacga tatgtccata atggatcgta
    14461 tgacttggta cttctgataa tcgtttgttt ccagattgat aaactttatc tgctagttgc
    14521 acagcttgat cgccacttaa acgaacgata ctgattgcac cttctccagg tggggtagaa
    14581 attgccgcaa tcgtatcaaa ttctaaagta atttcagcca ttttttcact tcctttttca
    14641 ttttgcgcac aaaaaaagtg cccactccac cctttatgca caataaacgg tgaacttcgc
    14701 actttgactg cttgttcttg attaaattct ggtcatagat tatcacactt gctataagga
    14761 atcaagtgtt taaaacaatt agatgaagtt gagcctgttc tgcttccatt accttatatt
    14821 taattgtcct tggatgtctc tttgcgttct ttggtttatt cgaagagaag atactgtata
    14881 aacaaggctt tatctttctt aaaaaaaacg gtacactaaa tggtagcgct atctaaacta
    14941 tacacttttg aaaggagaaa atatgtgagc ccgttcagat ctgctgcatt ggaagaagaa
    15001 agtgaccctc ggatcaacga tgtcgtttta tctacaggga atatcaacca gaaatatatc
    15061 gtccgcgata tcgtttttgt tgtggaatgc ttagaagctg atttgtttga cccatctttt
    15121 cccaaagatg accttttctt aacaacgaag aggaaattaa agacgaaggt acttgaatat
    15181 ggtgcgaatg cagtaatcaa ctgtcatttt gagtatcagc aaaaaaaacg cgacggaaag
    15241 tattttctgg aactttttgc ttatggaaca gtgatccaat ttatccaaac aacgattggt
    15301 taaatgaaac agacaaaaaa agcggatcaa aactgaagac agtttcgatc cgcttttttc
    15361 tatcaaagtt agtgactact tcatcgtata ctctgataag aatgatttct agtaaaatat
    15421 acttggtttg aaaaactttg tcaacgtgat ttattcaaaa agtaatggaa tcaataatat
    15481 tttttagcag gttctactac taaataacga taaggttcct caccttccga atgtgttttt
    15541 acatgttcat ctttgcttaa tgcagcatgg atttgtttac gttcaaatgc aggcattggt
    15601 tctaagaaaa caggctgccc tgtccgtttt actttttcag ccgtgcgatc agctaaacgc
    15661 tgaatgatct cttggcgctt ttcacggtaa ttgcctacat tgactactac agacagctta
    15721 tttcttgcta aacgatggat gaagacttgt gccagatatt gcaatgcatt cagtgttttt
    15781 ccatgtttcc caatcagtat gccttgcttt tgtgtttcta aatggaaaac aaccatatta
    15841 tttgattctc tttccatttt gacaagagct ggagcatcaa gctgttttga aatttcagta
    15901 agataaactg ccaattccgt taaagctgct tcatcaccta gctcacttac ttcttctgtt
    15961 ttttcttcaa gaatcgtttc gttgactacc tcgatctcag ctgttggttc ttcttccaaa
    16021 atttcttcta cagtctgttc tactgtttca gaaacttttt cgctgatact tggctggatc
    16081 gaaagccgtg cattctttcg acccattcct aggaaacctt tttttccttc ttccagtatt
    16141 tcgatttcga cttggtcttt ggccaaaccc aaatcagcca gacctagttg gacagcttcg
    16201 tctattgttg cagcttcata aatcggcatt agcattacct cccctattat ttccgttttt
    16261 tcttaggatt cattgctttc ttaagagcac gttctctttc gcggattctc cgtgcttctt
    16321 cttcacgctc ttttcgaatt ttgaaagggt tgttgatcag cagcgtttgt acaacttgga
    16381 aggcattgga aactacccag tataatgaaa gaccgctggc taaattgaca cccatcacta
    16441 aaatcattac aggcatcacg aaattcatga tcttcattga tgcattcgat tcaatttgac
    16501 tcatgctaga taaatacgta cttgcaaaag taaagattgc tgctaaaatc ggcaaaataa
    16561 atgttggatc cgcgttccct aagttcaacc ataagaaatg cccttgtttc aactctggta
    16621 cacgtgaaat agattgccat aacgccatca agattggcat ttgtactaat aaaggaagac
    16681 aaccagcata tggattgacg ccattttctg catataaacg ctgctgctct tctcttaact
    16741 tactttgtgt ttccggatct cttgaactat attgttgctg cagtgctttt aatttcggtt
    16801 gtaattcttg cgttttacgc atgctctttg tttggaaatg catcaacggc aataggatca
    16861 cgcggatgat cagtgtaaat aaaatgatcc ctatccctgc attaccaaat gacaacgcct
    16921 tgatcgcctc agcaaagtaa taaacaatat aacgatccca aattcctgta ctctgtgcgc
    16981 tgacttcccc agttccgcaa ccggataaga cgatgaccaa actaacgata ccggccatca
    17041 aaagtaaacg tttgtatttc ttcacttact cttttcctct ctgtcgatta tttttgcaag
    17101 ttttaagacg tggactaaat tggagcgaac ctcatcataa gtcaacccct tcacacttgg
    17161 tctagcaatc acaataaaat ccaactccgg atcaatttct tcttttatag aataaactgc
    17221 tgctcggatt tttcgtttga cttcattgcg gtttaccgcg ttgccgacct tttttcctac
    17281 agataaacca actcgaaaat gaggctgtcc cggtttttcc aaacggtaaa cgacgaattt
    17341 gcggttagca aaagaatctc ccgtatggaa cacggcttga aactctcgtt ctttttttac
    17401 tcgatacgct tttctcatta gttttctttc cttttctatc tctcacttac gctcataatc
    17461 ataccataat tacgattgtc ctttcatcat ttccctaaaa aagagtataa aaaaaaccac
    17521 tgaaacccca gtggcctaag ctgaaagaac ttttcttcct ttgcgacgac ggctagctaa
    17581 cacacgacgt ccatttttag tactcatacg tttacggaat ccgtgaactt tttgacgttt
    17641 acgtttattt ggttggtatg ttcttttcat atattccacc tccaaaatgt tcttatctat
    17701 tttataaaca cagacatact ttgatagtat aacgacaaag tagtgatttt gtcaatataa
    17761 aatagaagat cgattgttaa aacttatcca caagattatt tttttctatg ggaatcctat
    17821 gtggaaaaag ttatgcacag ggattatcca cagataataa aaggctggtt ttacacagaa
    17881 aacacacgtg taaaaaaagt aatccacaat caggcgattt cctgtgtatt ccgtctccta
    17941 actattggta tatctgtatt ttttatccac agacgcaaga ggaaatctat tggtttttac
    18001 gagttttcca cctgtgcatt agtggtgtgg aaaacttttt ttgacaactg tgtttttctc
    18061 ttttttcttt ttgtcgattg tgtggataat tgtaattaaa aaattttttt cttcggtttc
    18121 tgtggaaaac tattgaaaaa aatgctaaat tagtactagt tattcacttt ttaatgaagg
    18181 aggaggcctt atatggtatc cctcgatgct ttatggaatg aattaaaagc aacataccaa
    18241 aaggatttat cgcctgctag ctataataca tggatcgaaa cagcgaaccc tcgaaccctt
    18301 gatcaaaacc agctcgttgt tgaggttcct agcaagattc ataaagaata ttgggaaaaa
    18361 aacctggcga caaaaatcgt tgaagtcggt tatatgttat caggaaatga gattatccct
    18421 cgctttatta ctggcgaaga agcagagcaa gaagaagtta tagaagaaaa aaatccaaaa
    18481 gttgtggcac ctagtccgct aaaaaaagcc atgctgaacc ctaaatatac ctttgatacg
    18541 tttgtcattg gtaaaggaaa ccagatggct catgcagcag cgctcgttgt agcagaagat
    18601 ccaggttcta tttataaccc gctcttcttt tatggaggcg tgggacttgg taagactcac
    18661 ttgatgcatg caattggtca tcaaatgctg caaagtcagc ctaatgccaa agtaaaatat
    18721 gtaagcagtg aaacttttgc taatgatttc atcaattcga tccaaaacaa aacagcagaa
    18781 gagttccgtc aagaatatcg aaatgttgat ttactgctag tggatgacat tcaatttttt
    18841 gcagaaaaag aagctacgca agaagagttt ttccatactt tcaatgcctt gtacaatgaa
    18901 gggaaacaaa tcgtactaac cagtgatcgg ttgccaaacg agattccgaa actgcaagaa
    18961 cgccttgttt ctcgctttgc atgggggctg tctgttgata ttacaccgcc ggatcttgaa
    19021 acaagaacag cgattttacg taaaaaagcg gcggctgaac gtctagagat cccagatgac
    19081 acactaagct acatcgcagg acaaattgat tctaacattc gtgaattaga aggtgcgctt
    19141 gttcgagtgc aagcttttgc tgcgatgaat agtgaagata tttcgactag tctagctgca
    19201 gacgcactga aaacgttaaa atcaggaaaa ggacatcctc aattgtcaat cctgcagatc
    19261 caagaagaag tggcaaagta ttatcacatc caattgaaag atctcaaagg aaagaaacgc
    19321 gtcaaatcta ttgttgttcc tagacaaatc gctatgtatt tggcacgtga gctcacagat
    19381 aattctttac cgaaaatcgg agctgaattc ggtggcaagg atcatacaac ggttatccat
    19441 gctcatgaaa aaatccagca gctgatggat tctagtgtca gtatgcaaaa tgaagtatca
    19501 gaaatcaaaa actttttact aaattaattt gtcgattcta tcaactgaaa atagcatgtg
    19561 gataaaagaa aaaaacgttc aaaagttata cacacttttt ccacagctgt tttttcgttt
    19621 ctctcttacg ttttttgagt tttccacaga atcaacatgc cctattactt ttattattct
    19681 ttttaactaa taataaataa acaaacattc aaaggagata catcatgaaa gttactttaa
    19741 accgagctag ctttatgcag gaattgcaaa ctgttcaacg agctatttca agcaaaacca
    19801 cgatccctat tttgacaggt gtaaaaatca cactgacaca agaaggtttg actttgacgg
    19861 ggagcaacgc tgatatatca attgaaactt ttttgtctgt tgaaaacgaa aaagcaaata
    19921 tgcaaatcga atctactggt tccattgttt tacaagcacg tttctttagc gaaatcattc
    19981 ggagacttcc tgaagaaaca tttactttag aagttttaga aaataaacaa gtagcgatca
    20041 cttctggaaa agcgaatttt atcgtaaatg gattagatgc agataactat cctcatcttc
    20101 ctgttgtcga aagccataac cagatgaaat tacctgtaca cgtattgact aaactaatca
    20161 acgaaacagt ttttgctgtc tcacaacatg agagtcgtcc aatcttgaca ggtgtgcatt
    20221 tcattttatc tgataattct ttattagctg tagctactga ttctcaccgt ctaagtcaac
    20281 gcgtgattcc agtagaacaa gcggctgatc attttgatat tgttattcct ggaaaaagtt
    20341 tgatcgaatt atctcgctca ttaacaaatg aagaagaaat cgtcgaaatc agcattatgg
    20401 aaaaccaagt gttgttcaaa acagaaacga tgtatttcta ttcccgtttg ctagaaggaa
    20461 actatccaga taccaatcgt ttgattcctt caagttttaa tacagaggtt gaattttctg
    20521 ttcctagctt tttagcggcg atcgaacggg cttctttact ttctcatgaa ggccgtaaca
    20581 acatcgttcg tttatctatt cgtccagatg cggttgtttt atacggaaat tcgccagaaa
    20641 tcgggaaagt agaagaaagc ttgagttata cagcaagcag tggcgatcca ttagatattt
    20701 ctttcaatcc ggattatatg aaagcagcgt tgcgtgcatt tggagatatg agcatcaaag
    20761 tgaaatttat ctctgctatt cgtccattta cattggaacc aacggaagat ggcgttcaat
    20821 ttattcagct gattactcct gtacgtacaa attaacaatg attttcaatg ttcttgaaaa
    20881 tttaattgaa aagaaataga aaaaataaaa ttttaaacag aaggccctaa aatct
//
LOCUS       DAFGZE010000050        20363 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.052, whole genome shotgun sequence.
ACCESSION   DAFGZE010000050 DAFGZE010000000
VERSION     DAFGZE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.052"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            292..1431
                     /locus_tag="LTU54_001935"
     CDS             292..1431
                     /locus_tag="LTU54_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HBL8296645.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="LTU54_001936"
     CDS             complement(1498..2073)
                     /locus_tag="LTU54_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBL8296646.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="LTU54_001937"
     CDS             complement(2078..3052)
                     /locus_tag="LTU54_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBL8296647.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="LTU54_001938"
     CDS             3335..4303
                     /locus_tag="LTU54_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296648.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="LTU54_001939"
     CDS             4416..4694
                     /locus_tag="LTU54_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBL8296649.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="LTU54_001940"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="LTU54_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBL8296650.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5151..5906
                     /locus_tag="LTU54_001941"
     CDS             5151..5906
                     /locus_tag="LTU54_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296651.1"
                     /translation="MKRVKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSL
                     AEHQLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="LTU54_001942"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="LTU54_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBL8296652.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="LTU54_001943"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="LTU54_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBL8296653.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="LTU54_001944"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="LTU54_001944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBL8296654.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="LTU54_001945"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="LTU54_001945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBL8296655.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="LTU54_001946"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="LTU54_001946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBL8296656.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="LTU54_001947"
     CDS             10643..11185
                     /locus_tag="LTU54_001947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBL8296657.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="LTU54_001948"
     CDS             11208..12764
                     /locus_tag="LTU54_001948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBL8296658.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="LTU54_001949"
     CDS             12778..13680
                     /locus_tag="LTU54_001949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBL8296659.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="LTU54_001950"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="LTU54_001950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBL8296660.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="LTU54_001951"
     CDS             15123..15545
                     /locus_tag="LTU54_001951"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBL8296661.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="LTU54_001952"
     CDS             16014..16244
                     /locus_tag="LTU54_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HBL8296662.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="LTU54_001953"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="LTU54_001953"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBL8296663.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="LTU54_001954"
     CDS             17655..17831
                     /locus_tag="LTU54_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBL8296664.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="LTU54_001955"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="LTU54_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBL8296665.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="LTU54_001956"
     CDS             18326..18709
                     /locus_tag="LTU54_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296666.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="LTU54_001957"
     CDS             18878..19762
                     /locus_tag="LTU54_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8296667.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19876..20112
                     /locus_tag="LTU54_001958"
     CDS             19876..20112
                     /locus_tag="LTU54_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HBL8296668.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="LTU54_001959"
     tRNA            20200..20280
                     /locus_tag="LTU54_001959"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="LTU54_001960"
     tRNA            20287..20358
                     /locus_tag="LTU54_001960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6760 a         3567 c         4425 g         5611 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccagta tac
//
LOCUS       DAFGZE010000051        19694 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.053, whole genome shotgun sequence.
ACCESSION   DAFGZE010000051 DAFGZE010000000
VERSION     DAFGZE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.053"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..210)
                     /locus_tag="LTU54_001961"
     CDS             complement(<1..210)
                     /locus_tag="LTU54_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296669.1"
                     /translation="MLFNETQLWFKFDPSNRFIKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDI"
     gene            complement(216..446)
                     /locus_tag="LTU54_001962"
     CDS             complement(216..446)
                     /locus_tag="LTU54_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296670.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENNQ
                     PVIAKKETVLSEGKIKTRYILEVETKFKNRSE"
     gene            1063..1725
                     /locus_tag="LTU54_001963"
     CDS             1063..1725
                     /locus_tag="LTU54_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296671.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            1804..5031
                     /gene="fss3"
                     /locus_tag="LTU54_001964"
     CDS             1804..5031
                     /gene="fss3"
                     /locus_tag="LTU54_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HBL8296672.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITDAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            5145..5459
                     /locus_tag="LTU54_001965"
     CDS             5145..5459
                     /locus_tag="LTU54_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL8296673.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            5472..5846
                     /locus_tag="LTU54_001966"
     CDS             5472..5846
                     /locus_tag="LTU54_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBL8296674.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            5856..6200
                     /locus_tag="LTU54_001967"
     CDS             5856..6200
                     /locus_tag="LTU54_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL8296675.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            6271..7623
                     /locus_tag="LTU54_001968"
     CDS             6271..7623
                     /locus_tag="LTU54_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HBL8296676.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFWVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            7683..8825
                     /locus_tag="LTU54_001969"
     CDS             7683..8825
                     /locus_tag="LTU54_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBL8296677.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            8850..9104
                     /locus_tag="LTU54_001970"
     CDS             8850..9104
                     /locus_tag="LTU54_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296678.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            9360..10544
                     /locus_tag="LTU54_001971"
     CDS             9360..10544
                     /locus_tag="LTU54_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL8296679.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            10541..10678
                     /locus_tag="LTU54_001972"
     CDS             10541..10678
                     /locus_tag="LTU54_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HBL8296680.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            10683..10904
                     /locus_tag="LTU54_001973"
     CDS             10683..10904
                     /locus_tag="LTU54_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296681.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            10917..11417
                     /locus_tag="LTU54_001974"
     CDS             10917..11417
                     /locus_tag="LTU54_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBL8296682.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            11514..13361
                     /locus_tag="LTU54_001975"
     CDS             11514..13361
                     /locus_tag="LTU54_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBL8296683.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            13364..14260
                     /locus_tag="LTU54_001976"
     CDS             13364..14260
                     /locus_tag="LTU54_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8296684.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNFYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            14310..14699
                     /locus_tag="LTU54_001977"
     CDS             14310..14699
                     /locus_tag="LTU54_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL8296685.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            14686..16188
                     /locus_tag="LTU54_001978"
                     /pseudo
     CDS             14686..16188
                     /locus_tag="LTU54_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            16933..18759
                     /gene="ltrA"
                     /locus_tag="LTU54_001979"
     CDS             16933..18759
                     /gene="ltrA"
                     /locus_tag="LTU54_001979"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBL8296686.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
     gene            18893..>19694
                     /locus_tag="LTU54_001980"
     CDS             18893..>19694
                     /locus_tag="LTU54_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8296687.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         6655 a         3219 c         4308 g         5512 t
ORIGIN      
        1 gatatcaaac aaaaagtata catctttctt cattctagtt tttgcagcag ggattttaaa
       61 gtagttctta ttagaatagt agagattgat taataagcta tcttcgattg ctagaaagaa
      121 aacttctgaa tcccacacct tataaaaatc tttgataaat ctattcgaag gatcaaattt
      181 aaaccataat tgtgtctcat taaaaagcat agccattact ccgatctatt tttaaactta
      241 gtttctacct ctaatatata tcgagttttt attttgcctt cagataatac tgtttctttt
      301 ttcgcaatta caggttggtt attttcggaa aaagctaata tagctaagat tgaaacatcc
      361 atctggaatt tatctttttt gctgctttgc tcataaaagt ctgcatattc atcactgata
      421 ttttttctaa taaagtcttc catcataaaa atcacctcaa aacgattata cgaacttacg
      481 ttctcgttgt aaagcgatat ttgaggggca aaaagggggc aaaaatacgt ttacctttga
      541 tatatctcag attgaacata gtgtttatta tatgtcggta ttgttgattt atagctgttt
      601 tgagggtatt agatacataa tagaatatag tggttttgtt tacgtgcatt acacggaaaa
      661 gctgctcgta tcaaagaaat cagaagataa acacggaaaa cttccttgga aatctgaaat
      721 ttgtgaaaag tccttgtata tcaagggctt tttctttttt ctctatcaca gttttaagcc
      781 atttttggct gttttggacc atttttcaat catattccct taaaaattgt cctagattgt
      841 ccgacattga ctatcagtgt cgtcaaaata gggcaaaatt tagtagtcag cttgacgaca
      901 aatataatac gaaaaatacc aaaacattta ccgaatttag caacttttga acccaagttt
      961 ctcaaagggt cagctattag caaaaaagac aaaacatgta cttttctact aaaaagttga
     1021 tgtgataggc ttctatctaa ataactatta ggagttttta tcgtgaagca aaatgatgga
     1081 cgtatcattt ggaaaaatca aagtgagtta aaactgattt taacaattaa tgaattcatc
     1141 gaaaaacacg gaattacatc tagccgtcaa taccagaaaa agctatcaga aaatccaaat
     1201 tctgcaccaa gtatgtggtt cattaacaaa aaatatggat catgggagaa tttattgatt
     1261 agtataggac gagaaaatac tgggtatgga aaatgggcta gaatgtcaga acaagaatta
     1321 ttggaaatag tagaagcctt tattaagtgc gagaagataa cctctcaacg aatgtacgaa
     1381 caaaaatcag tagggaagaa tattccttca cttagtacca ttaaaaagat gttaggagat
     1441 atacgtccac tttttaaaga aaaaaatgat ggatctcgat ttactgattt cgaactgctg
     1501 ttagaactaa aaaatgaaat aatccgatta aaactacagg atgatttatc aatgacaaag
     1561 tttcgaaaac tagttcaatc cccaaaacta ccatcagttg atacaattat gaaaagaacg
     1621 aacaaaaatt gggaggaatt gatggcagag attggatttg attatcgaag aatcaaaatt
     1681 tataagcaga gaaacaacct atccaagaca aagaaaacta aatagtatca ctctaaatca
     1741 gacgttcaaa attaaaattt ggacgtcttt tttattaccc aaaaacgaaa ggagatttat
     1801 tttatgaaaa agccaagatt taagaattgg cgtttgctcg caacactgag tctattatgc
     1861 caaacaattg gtggatcgct tggcccaacg attgcctttg ctgatgaaat tactcatcca
     1921 caaacggtaa ctgtggaatt ggatttggct caccagtatg ctgtcgaagg tacatttagc
     1981 gatggtcgtc ccatgtcaga agttaccgtt ccgcactatg cagtttacaa tggtgtgaag
     2041 caagatattt tctgtattga gccaggggtg ccgatttaca atgagttcac tcctggttat
     2101 gagaaaaatc cattgcctga tatgtcggag aaagcgaaat tagtttccgt tttatggaag
     2161 gaagctggta cggatgtgga tacacatatt gtggcccaga agatgatttg gcaagaagtt
     2221 aatggctaca cgcttcactc aattaaacgt tctgatggta gtgcagtaaa tatcgcagcc
     2281 attgagtcaa aaatcaatca agccattgcg gattatcaaa agaaaccaag cttccataac
     2341 tccacagtga aaaccgtgtt aggtcaatcg actactgtga cagatacgaa cggcttgaat
     2401 ttatcggagt ttgatgaagt tgtcgagaat atcgcaaata ttgattatcg tgtgaacggc
     2461 aatcaactag tgattactcc aaatgccaat tcaaaagaga gtggtgaatt aaccctaaag
     2521 aagtcagcta atacaggaac accggtagct tataaaatgg ctggtcaaca aactttgatg
     2581 gcgggggcaa ttgataagcc caatacctac acaatcaaaa ttgatgtgga aactgagggt
     2641 tctttgaaga ttaaaaaagt cgataaagaa acaggaaatg ttgtaccggg aaccgttttc
     2701 catttagact ttggaaaaac attacctgca aaagacgtga ccaccgataa agaaggcatt
     2761 gcgacattgg atgagattcc tcatggcgcg aaagtaacca ttactgaaaa atcagtgcca
     2821 gctccttata cgattgacac tacaccaatc accgcaacca ttaaatctgg tgagactatt
     2881 tctgtgactt caaaaaatac tcgcgaaaaa ggtcaaatca ttttgaataa aatgggggtg
     2941 gaaacaggaa ctgatctttg gaacgacaaa tactcattag ccggaaatac gtttgccatt
     3001 cgcaaagaca gtccaaccgg tgaaattgtc caagaaatga ccacggatga aaaaggtcat
     3061 gcggaaacac caaaagagat tgccaatgcg ttggaattgg gaacctatta tgtgaccgaa
     3121 accaaagcta gtaatggctt tgtgaatacc tttaaaccag taaaagtcga attgaactat
     3181 gtcaatcaaa ccgtggctct tgttaccagt aacgtaaaag ggcaaaacca agaaatcact
     3241 ggtgaaacca ctttgacaaa agaagacaaa gataccggta atgaaactca aggtaaagct
     3301 gagtttaaag gagctgaata tactctcttt actgcaaaag atggtcaagc ggtcaaatgg
     3361 agtgaggctt ttaaaccaga gttagtgaag ggaacgaaag cttctgatga aacggtgact
     3421 ttgactttag atgaaaagaa tcaagttgct gttaaacact tagcgattaa tgagtattac
     3481 tggcaagaaa ccaaagcacc tgaaggatat accttggatg aaacaaagta ccctgtttcc
     3541 atcaaaaagg tggatgataa cgaaaaaaat gccgtgatta cccgagatgt tacggcaaaa
     3601 gaacaagtca ttcgctttgg ctttgatttc tttaaatttg ctggatcagc tgctgggact
     3661 gccgaaactg gcttcaatga tctgaccttt aaagtatcgc cattggaagg aaccagtgag
     3721 attacagatg ccgaagatga agcaattaca gcttataacg agcaactagg ttttgatggc
     3781 tatggcaagt ttgaaaatct tccttatggg gattatttac ttgaagaagt agaggctcca
     3841 gaaggatttc aaaaaattac gccattagaa atccgttcta cttttaaaga aaataaagcg
     3901 gattacacta agagtgaata catttttacg attaccgaaa aaggacaaaa acaaccaatt
     3961 aaaacggtga ccgttcctta cgagaaactg acgaataaag cattttctgt tagcttgaat
     4021 cgtttgatgc tctatgattt acctgaagaa gaagatagct taacttctct tgggacttgg
     4081 aaagacggag gcaaagaatt gacagacctt gattctaccg agctagttga taaattgagc
     4141 tataacttgc atgaaatcaa agaagactgg tatgtggtag cgcaagccat tgatgtggaa
     4201 gcgacaaaag ctgcccaaga aaaagatgaa aaagccaaac cagtggtaat tgccgaaaca
     4261 accgcaacgt tggcgaataa agagaaaaca ggaacttgga aaattttgca taaattaacc
     4321 gcagaacaag ttttggataa aaccatcgtc ttattcaact atgtgtatga aaacaagaaa
     4381 ccctatgaag caggtgatga tccagtagcg aaagatgcta gcttgaacaa tcaagcccaa
     4441 actgtcaatt gtacggtgga acgccatgtt tccatccaaa caaaagccca cctagaagat
     4501 ggttcgcaaa cctttactca tggggatgtg gtggatatgt tcgatgatgt gtcgattacc
     4561 catgatgtac tggatggctc aaaagaagct tttgaaacaa ttctgtatgc attacttccg
     4621 gataatacga ccaaagaaat ttggaaatct ggcaaaattg attatgaagt gaatgacaaa
     4681 gaattcacta aaaccgtact tgcggaaaaa gtggacacaa gtaaatatcc agaaggtaca
     4741 agttttacct tcaaagaaat taactatggc aaagacggaa acatcaatgg gaaacacaat
     4801 gaggatctaa aagaaaaaac tcaaacttta acacctaaaa aagttccgac aacaccaagt
     4861 gcaccagaac aaccggaaat accaactgtt ccaagtgact ctcaagaatc tagtcctaca
     4921 gtaaagacat tcccacaaac aggggagaaa aattctaatg ttctgctgtt tattgggttt
     4981 accttgatct ttgcgactgc tgggtattat ttctggaatc gccgaaacta aggtgatgtg
     5041 atgaaacaac taaataggaa aaaccaacgt gcgcgaccgc ctcctaaaaa aagtggtcgc
     5101 gctatttttg tatccaaaac taaaaatgaa aagaggaaaa gaagatggaa ttgaaatttg
     5161 ttgtgccaga tatggccgaa acctttggaa agataaggta tgccggcgaa ggcgaagttt
     5221 taactgaagg atatgggcgg aatactacag taattggtcg tagttatcat ttgtattcca
     5281 gtaagcaacg agccgatgat attgaagtgg tggtagctgc ggaagccggc gaaaaggatt
     5341 ttgatcaaga tcaaccacta aaagccgtga atccccattt ggttgccaaa ggctatgaga
     5401 ttgagaatcg tgggtttacc gactacgtgt tgtatgtcga tgatttagtg aaagcatagg
     5461 aggaaaagaa gatgagatta acagaaggta ttgttgtaga ttcaggacta acgtttggga
     5521 aattacgatt ttccgcatta cgccgagaag tacggaagca aaatgaggat ggaacggtta
     5581 gtaacgaggt aaaagaacgg acttataatt tgaaatcctc tgcgcaaggt cgaatgatcc
     5641 aagttagtat cccagcaaat gtacccttac gcgagtttgc ctatgacgca gaggtagagt
     5701 tggtcaatcc aatcatggat acggtcgcca attatgtttt ccgagaagga actactgtca
     5761 attggttcat taaggccgat gatttagtct tgaaacgaca accagatcag ggaaattcta
     5821 caaaccaaaa tgaaggaaag aaataggtga agcgtatgga aattaatgtt gaacatattt
     5881 tagattgtct cgatcaatat ggaaaaggtg agctaacgga ggaacaactc actaaagcat
     5941 taacctatga tgaaaaaatg tttctgatta tgcatcaagg gttactggaa gtaggtaacg
     6001 atgccgaaga agattttgat gtactgaatt ggttagggga agaagagtcc ttctttatgg
     6061 ttatcgaagt aaatgaaagt ctatgtcgag aagccgaaac cgtattggaa gaaattggtg
     6121 tggaaatgcc ggatgccatt gaagtttttt taaagcaact agtggaaacg aaacaactcc
     6181 ctatagcagt aaatgactag cataactgct tgaaattgga tcaatgaaat cctatgaagc
     6241 ttttttcgat tggaggtgga aaccataaaa atgtacaaag ggcatcgtat acgggcagga
     6301 gatcagcact tggtttatca ctttgttctt ggctggctac tcgctctgtt catcggttgg
     6361 atgagcgtct tttattttca agagcttaga caatttgata tttctaagct atcactttct
     6421 actatagaaa tcgttcgttc tataaaagat ttaatctatt tgctaggaag tctcgttctt
     6481 tcaggaagca cgatgttgct ttatatacac ttttttcagg atcattggcg cagtttgtgg
     6541 catcgacaaa agctagcccg gatgattcta gaaaatcatt ggtacgaagt aaaacaaact
     6601 cagagtgaag gatttttcaa agacctcaat agtagtcgaa ccaaagagac aatcagttac
     6661 ttccccaaaa tctattaccg tatgaaggat ggtttacttt ctattcgcgt tcaaatttca
     6721 ctgggaaaat atcaagatca gcttttaaag ctggaaaaga agttagaaag tggtctatat
     6781 tgtgaactag tagaaaagga actcaaagac tcctatgtgg agtacacttt actgtatgat
     6841 ataattgcca atcgaattgg gatagacgaa gtagtggcag aaagtggggc tttgcgattg
     6901 atgaaaaatc aagtatgggc ttatgattct ttacctcata tgttaatcgc tggcggcaca
     6961 ggtggtggga agacttactt tttactcact atcatcgagg ctttattaaa atctgatgcc
     7021 gagctttttg ttcttgatcc caaaaatgcg gacttagccg atttaggtac ggtaatgcct
     7081 catgtctatt cccaaaagga ggaaatttct gcttgtgtgg aagatttcta tgagcgcatg
     7141 atgactcgta gcaaggcaat gaaagaaatg cccaactaca aaacaggaga gaattatgcg
     7201 tttcttggac ttccaccaaa ctttttaatc tttgatgaat acgtggttta tatggaaatg
     7261 ttaacgacga aggaaagtgc ggtgattttg aataagctca aacaaatcgt gatgttaggt
     7321 cgtcaatctg gtttctttct gattctagct tgtcaaagac cggatgcgaa atatttagga
     7381 gacgggattc gcgatcaatt taatttctgg gtggctttgg gtcgtatgag tgaactggga
     7441 tattccatga tgtttggcga ggttgataaa aaattcttta tgaagcgtat caaaggtcga
     7501 ggctatgtag atacgggagg gagtgtgatt agtgagtttt atacgccact tgtaccaaaa
     7561 ggatatgatt tcttaaggga aattagcgac attggtggat taaacgttaa tacggaagga
     7621 taaaataata gtatgtagcc taggagtgtc aagaatcctg ggcttttttt acgaggtgat
     7681 ttatgacatt tttagattta ttcgctggga ttggtggctt ttgcttaggt atggaacaag
     7741 cgggtcacca gtgtatcggc ttttgtgaaa ttgatgattt tgctcgccag agttataagg
     7801 caatccatga tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagacgagt
     7861 ttattcaatc tctcggacca gtggatatcc tttgcggtgg atttccgtgc caagcttttt
     7921 caattgcggg aaagcggcaa ggattttccg atactcgagg tactttattc tttgaaatcg
     7981 ctcggttcgc cgctattctc caacctaagt ttttattcct tgagaacgtc cggggattac
     8041 tcaatcacga aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt
     8101 atgatgtgga atggcaagtg cttaactcaa aggcctacgt tccccaaaac cgtgagcgga
     8161 tcttccttat cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggat
     8221 cgtctccaac atctgatcaa aacatcagaa acttattaaa tatcaaccct tccaatcgag
     8281 gaatgggggg acaagtctat gggtcagatg acgtagcgcc taccttgacc ggtgatgaag
     8341 gaatcaaaat cgcattgccg gtagatgatg gaatttctgt agcgggtatg ttactgggaa
     8401 attttgaaca aggcaatcgg gtttatgaga taactggaac agcgcctact ttgtcaacga
     8461 aacaaggcgg aacgaagatc atgcttcgga aaaacgaaac agaatatcaa gaggtgaagc
     8521 cagggaacag tgtgaacctg gcttttccta attccacaag tcgtcgaggc cgtctgggaa
     8581 aacaaagtgt gcatacctta ttaacaggag accaacaagc agtcgtcacg gatcagtatc
     8641 aaattcgaaa gttaacacct agggagtgct ggcggcttca gggctttccg gactgggctt
     8701 ttgatcgagc ttctcaagtc aattcagata gtcagttgta taagcaggct ggaaattctg
     8761 taacagttcc ggtcattttt gatattgcta gaagattaaa ggaggtaaaa acagatgatt
     8821 tatgatgagt tgattgggga atctactggg tgattggcaa gattcagtca gatccggaat
     8881 tggaagaaga actgtaccgg ctcaattttg atattcgcaa aaacggggtg aaagtccctg
     8941 atgatccgta tgtaattgat gaggaaacgg atgctcgaat ggagctgaat caagtcatca
     9001 gtgaattgga acgaattgcg gactttgcta aagaacctga tatccgccaa tacttgtttg
     9061 agataaaagc agagttggaa attgagggta ttaccgcgga gtaacggcca aataaaccac
     9121 tgataggcac tgaccgcggg ggatttgcga agcagaaatc ctccgtggtg ctgtgcctgt
     9181 ttatggacgg cgttagccgg ccatgatgtc tcaccccccg tatctaacag gggggtacaa
     9241 aaacacaaaa aataagcaag caacgataag gaatggctgt cctatcaagg ataataagca
     9301 atttgtgtag atgttaactg atttgaaaaa gttaacacct taaatttgga ggtgagaact
     9361 tggcacaaag gaatttagat tatcggctat taaaagaccg gcgtaatgaa tatggcgttt
     9421 cacaaaataa attagctacg acttgtggac ttagtcgccc gtacttaaat cagatagaaa
     9481 atggtggcgt aaccgcatcg acaaaaacca tgaggaaaat ttttgaccaa ctggaaagtt
     9541 tcaatcccga gttaccttta acgttactgt ttgactatgt aaggattcgc tttcccacaa
     9601 cggatgcgag gaaaatcatt caagagattc tccatttgaa atttgattat atgctccacg
     9661 aagattacgc cttttactcc tatcaagaac aatatgttat gggagatatt gtggtgatgt
     9721 tgtcacatga agaagataaa ggcgtccttt tagaattaaa gggtcgtggt tgtcggcagt
     9781 ttgaaacttt tttactcgcc caaaagcgta gctggtatga ctttttcgaa gattgtctaa
     9841 aagccggtgg cgtgatgaaa cgcttagacc tggcaatcaa tgatcgagtt ggcctgttag
     9901 atattcctga tttaacgaaa aaatgtcaga aggaagaatg tatctccttg tttcgtacct
     9961 tcaaaagcta tcgttccggg gaactgttga aacctgatga taaagatggt atgggcaata
    10021 ctttgtatat tggcagtctt aagagtgagg tctatttctg tttgtacgaa aaagactatg
    10081 agcaatatat caagttaggg attcctttag accagaccga aacgaaaaat cgttttgaaa
    10141 tccgactgaa aaatgatcga gcttatcatg caattcaaga tttgttaaaa ggtcgcagta
    10201 ttgaaagtac cacattttcc attattaacc ggtacttgcg atttgcggat aaggtggaag
    10261 gcaaaaggcg aactaactgg cccttgaatg aacaatgggg gcgcttcatt ggacggaatc
    10321 agaaggaaat tcaattaact tctgaaccga agccttatac cattgaacga acgttgaact
    10381 ggcttggacg acaagtcgcc cctacgtgga agatggccaa ggagttggat cggttgaatc
    10441 agacaacgta cattcaggat atggtaaata atactcgact atcagaccgg cacaaaaaga
    10501 ttttggagca acaaagtatg gcaattgaaa atttgattat atgaaaagag gaatcgtgat
    10561 ggaaattgtg aaagatattt tattggtgtt tggtggcagt atgcttggtg ttactgtgat
    10621 gtgcttgatg catgcaagtg cagcggcgga ccgagcaatg gaaaagaaag gaaagtgatg
    10681 aaatgaattt cggtcaaaat ttgtatcaat ggtttttaac caatgctcaa tctttagtct
    10741 tactagcgat tgtggtcatt ggtttgtttt taggatttaa acgcgagttt tccaaactga
    10801 ttggcttctt ggtgattgct ttgattgcgg taggtctggt ctttaatgct tctggtgtga
    10861 aggatgtctt actcaatttg tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg
    10921 aacagatacg tgtttatatt gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg
    10981 catggtttac cccacccgtc gattttgatg aagtgaaaga acggattgga ttaaatgacg
    11041 aatatgaaga gtacgcaatt catgattatg aactgccttt tgagattgat gagtatacac
    11101 caattgagga aattaatcgg ttgtgtggtt tagcggaaga gcttaaagga acgccaattg
    11161 gtgaggtcgc ttcagagatt caacatgcct tcttcaattc tttcgaggaa atggtagagc
    11221 atgtggatga tatcatctat tatcccgatt gtaatgacat gagtgatgta gcgtattact
    11281 tgattgtaga agctggtgat ttaggcgaag taccagaaca cttgaagacg tatattgatt
    11341 ttgatgcgtg tggtcgagac ttggagattg gcgggaatta tttggttacg agtcgagggg
    11401 tttttgaact aatgtaaatc atttgttaga attgaccaga aatttctatc gtacttttac
    11461 ttttctattt gatatactga aatagaaaat atttgttgat aggggtgtta ctaatggttg
    11521 gcaataagtg gtacaaatgt gatcttcata ttcattcaac tgcgagtcag tgctttcagg
    11581 ataaaaaggt tactgctaag gaatgggtag aagaatgttt aaacaaagga ctcgattgtg
    11641 ttgcattaac tgatcacaat acaggggaaa atattgatga atataagaaa gtggctatcg
    11701 aaaatggctt agattttttt cctggagtgg aattaacgtg tggagaaaat ggaactcatt
    11761 tactagtcct atttgataaa agtgcatgta caaatgatat ctctgatttc ttaaccaaga
    11821 tgggtataaa aagagaggat tgggccagcg ctgcagcttg ctcaatgaaa tcagttttag
    11881 aagttattga ttttgctgag cacgagggct tgctcgttat tcctgcacat atagatgagt
    11941 ttaatggaat ttcttcagca aaatttggaa atctaggaaa gatatttgaa tcagaaaata
    12001 tatatgcagt acagacggtt caaaaagaat tttttgaaaa tcgctcacaa atgattccct
    12061 catctaagcg tgacactatc tatgatagtg tgaatgccag atatgatggt agagttggaa
    12121 aagatacttt ggaatcttgg tataaatcag ttgttgaggc agaaaaaaat aatatgacat
    12181 ttcttagctt ttctgataac ccacactcga aaggaaattc aaaacatgga ctttggggaa
    12241 taggcacaag atattcctat ataaaaatga aggacgaacc tgacttggga tcacttcgcg
    12301 atgctttaat gctaggcgaa actcgagttc attctgattt ttctaatttc tccattaatg
    12361 aaaatgaagt tttattggaa aaattaagct tttctgggac cactcttagc acaaaagaag
    12421 ttgtagtcga attcagcgat aatttaactt caattattgg aggaagagga acaggcaaat
    12481 cttgtattac aagattcttg gtctatgtcc ttggaaagga agcagaactt gatcaatttt
    12541 ctgaaataca aagtgattat cagaactttg cacaaattga acataatggc tctggaatat
    12601 ttttgaagga tacaattgtg aaactgaata ttttctataa ggggacaaaa tatcaaataa
    12661 taagaacaca ggatagacat tctatttatg aatttacaca agagaatggt ctggttgaaa
    12721 aagaaacaga acggttgaga atgatttccg ataaggtgag tatatactct caaaaacaaa
    12781 tttatgaaat ttcaaaaaat caaactagta tactagaatt actagatgga tataactctg
    12841 atttgatttc agagtataaa aatgagattg aaacatgtgt aaacgaaata agaaaattaa
    12901 attgggatat tgtttctgtg aaaaaagaaa tacaagataa ggcgaaggta gagcttgaaa
    12961 ttgaagattt aaaaaaacaa gtagaaaagc tatctcataa gagttataaa gatgtttatg
    13021 atgagtattc aaaagagaca gatatttata gggaactgaa acgtgatgtg gaggcactta
    13081 aggaaattcc caaaaatcta actgatagtg ttgacaaaat tgactttggc aatggtataa
    13141 aagttacaga cgaaattgat gaacaccgcg gagaattgat taagaattta gtgaccaatc
    13201 gggctaagat tcaaacaata attaatgaaa tgtcagatga aattgatagt tatagagcag
    13261 agctgaataa atcagattgg aaagttaact ataatgaggt ttcaaagagg tatcaaagag
    13321 gtatcaaaaa cttagtgaag agttatctca agatgaatta aaaatgttac aaaaccttga
    13381 ttctttatat caatctttat caaaaactga agagcgattg gaagaaatta ttcaacaaga
    13441 aataaagctt aaagattacc aagttgaggt tgactcagca atgtcaagaa tgaataaatc
    13501 atatcaaaat ttctattctg agagaagtag ttcctcgcgc gaaatttttt ctgatattac
    13561 aggaatagag gctactattc aaccaaatag ggatttcaat aattatgtta aagagctcag
    13621 agcttgtatt aataaggagg acacttttga cagtgagttt ggatctctta ttgataagct
    13681 gacaaaaaat tccattacta atcaagatat atataatgac atacttgata taagagttag
    13741 tggggaaagt agtatttttt cagataaaaa attaatcaat tctataagaa aattgaatga
    13801 tgcacaagtt cttgaattac aaacccttaa gccaaatgat ttagtagtta tccgacttga
    13861 actaaatgga aaaaaagtta gtttatcgaa tgctagtgct ggtcaaaaga catctgcaat
    13921 tctttctatg attttggcgt atggaaattc accactcata ttggatcagc ctgaagatga
    13981 tttggatagt caattaatta ataacttaat cgtaagaagt atcattacaa aaaaggaaaa
    14041 cagacaaatt ttaattgtaa ctcataatgc aaatatccca gttaatggtg attcagaatg
    14101 gttgattagc atgggagatt caaaagaaat atcggctgat cagtgcgggt cggtagatga
    14161 acccaatatc aagccaagaa tatgtactgt gatggaaggt ggcgaggatg cttttacaaa
    14221 tagagcgaaa cgctatggtt ttaaacaaat tgtagaataa cactagaggg tgagacatat
    14281 tattatgtcc gcccttttta ggaggaaaaa tgaaaaaaat aaaaagctat accagtatct
    14341 gggcagttga aaaagtcatt tacgcaatca atgattttca gttgcctttt cctgtgactt
    14401 ttaaccaaat ggcttggttc gttttatctt tactgtttgt catcgtattt gcccatgtgc
    14461 ctccactatc tatgatagag gaggtctttt taaaatactt aggcataccg gtggctgtca
    14521 cttggtttat gtcgcaaaag acgtttgatg gcaagaaacc actgggattt ttacgatcct
    14581 ttatcagcta tcacttgcga tttaaggtga cttttgcagg aaagaaagtc aaagaacaga
    14641 agaaacgatg ggatgaaccc agtactcttg taaggagtgt gaactatgta tccgattaag
    14701 tatattgaaa ataatctcgt ctttaaccaa gaaggcgagt gttttgccta ctatgaatta
    14761 gtgccttaca attactcctt tttgtcaccc gaacagaaat ttcaagtgca tgataacttt
    14821 cgccaactaa ttgcgcaaaa tcgtgaaggc aagatccatg cgttacaaat tgccacagaa
    14881 agtagtattc gcgccacaca agaacgatca aaaaaagaaa ttaccggtag actcaaagaa
    14941 gtagcgaaac aacgaattga tatacagaca gatgcattag tatccatgat tggcgatagt
    15001 cagattgatt atcgcttttt tattggcttt aaactaattg cgacagatga agaagtcaat
    15061 ctaaagagtc tcaagaaatc tttcttttct gggcttcaag aatttgtcta tggagtgaat
    15121 catcatttga tgggcgattt tgtttcctta tccaatgaag aaattcgctg ctattctaag
    15181 cttgaaaaat taatggaaag caaattggct cgaagattta aagtcaggcg agtcacaccg
    15241 agtgacctga cgtatttgat tgaacatatt tatggcgaaa aagggatacc gtttgaagaa
    15301 tacgaatttc agctaccaaa gaaaaagcta aaatcagaaa cactggtgaa acgttatgac
    15361 ttattacgtc caaatcgttg tttaattgaa gaaaaaccaa ggtgcttacg gatggaacat
    15421 gagaatcatg agtcgtacgt agcgtattta acaattaata caattgtggg ggagatggaa
    15481 tttccttcct cagaactttt ctattaccag caacaacaat ttacattccc aatcgacaca
    15541 agtatgaatg tagagattgt gacgaataaa aaggctttag ctaccgttcg taataagaaa
    15601 aaagaactga aagatttaga caatcatgct taccaatctg acaatgaaac gaattccaat
    15661 gtcttagatg ctttggattc cgtggatgaa ttggaaacca ccttggatca gtcaaaagaa
    15721 tctatgtaca aactgagtta tgtggttcga gtgagtgccg aatcagtgga tgaactaaag
    15781 cgacgctgtg atgaagtgct cgatttttat gatgatacca atgtaaaact agtccggccc
    15841 tttggcgata tgatggggct acatgaagaa ttcttaccct taagtaaacg atatatgaac
    15901 gactatattc agtatgttac gtctgatttt ttggcggggc ttggttttgg ggctactcag
    15961 atgctaggag aattagaagg gatttacttt ggctacaatg tggatacagg tcgtaatgtt
    16021 tacctgaaac cggcattggc ttcacaagga gtcaagggtt cagtaaccaa tgcgctggcc
    16081 gccgctttcc tgggttctct tggtggaggt aaatccttta gtaacaatct cttagtctac
    16141 tatgctgttt tgttcggtgg tcaagctgtg attgtcgatc caaaaggggg tgcgaaacgt
    16201 tcctacttga aaagagtagg cacagcactt cattaaatca atatttaatg tgtgttggag
    16261 aactaaacac acgaatagtc gaatgacggg gtaacgccct gaaaggctac tttaatactc
    16321 tgactggcag aaacttgagt aaacaaggct ctgtcagaag ctcggtgaag tcggcagaca
    16381 gacaccgtaa cagataatgc tgacgaaaga ttcccagagg tgggcgttgt gtcctatatg
    16441 ccggaagtct ataaaatatc tatggttaga atgtagatga gaggtctact gacgaatcct
    16501 tgaatgtaca ggtctatatc ggggctggat agaaatgtcc agtgacacaa acagtgtcgc
    16561 tactgtgggt gagtaagact ccgcatttat gaaaatccat ataacgttaa aggcgttatc
    16621 aaggtagcag gcttatagag gaaacctaag gatatatgta cagatagtgt gtttggaacg
    16681 ttgaaagctg acaacatgga gattttactg tctgtgaagt gttcacatca gaaagttatt
    16741 gtgagattag caatgatata aggtgtgtta atgtcagtga gagtggtggc acagtaccta
    16801 tgaaaccgtg ataacaagcg gtggagggat agccactagt catatgaaga atagaattac
    16861 taagaaaaat ggtatgggtt cgagtaagac taagagatgt acttgctcct gcaaacaagg
    16921 agggtacaga ccatgctaaa gaaaaacaag ctccgctata acgagtatta tgatatgcaa
    16981 catatttatg acgagttata cgcacagagc aagaacggta ataactttta taagttactt
    17041 gaaattattg gttcggaaca aaatatttgt ttagcttacc gtaatctgaa atcaaacagt
    17101 ggcagtaaaa cggctggaac ggatggaatg acgatagacg atataaaaca actcagcaat
    17161 gctgagattg ttgctaccgt tcgggaaagc cttagcaact accgccccaa atcagtcaga
    17221 cgtgttttca ttccaaaagc tgggagcgat aaaatgcgtc cattggggat tccgtgtatc
    17281 tgggacaggc tggtacaaca atgtatcctg caagtcctag agccgatatg cgaaccaaag
    17341 ttccataatc attcctatgg attccgtgcg aatcgaagtg cccaccatgc agtcagcaga
    17401 gtaacgacac tgataaattt atcaaaatat cattactgtg ttgacgtaga cattaaagga
    17461 tttttcgata atgtcaatca tggaaaacta ttaaaacaaa tctggacact tggtattcgg
    17521 gataaaagat tgatttgcat tatcagcaaa atgctgaaag ccgaaattga cggcgagggt
    17581 gtcccagaaa aaggaacgcc acaaggcggt ctgttaagtc cactgctctc cctcatagtc
    17641 cttaatgaat tagactggtg ggtaagcagt cagtgggaaa cattccagcc gaagaaccgc
    17701 agtaaaaatg ggtggcttca atacgcaaag aaatatacca aactgaaaag cggttttatt
    17761 gtacgttacg ctgatgattt taaaatcatg tgttcaacct atggagaagc acaaagattt
    17821 taccacagta ccgtagattt tcttaataaa aggctgaaat tagaaatcag tccagagaaa
    17881 tcaaaggtag taaatctgaa gaaaaattca tcagattttc tggggttcaa aatcaaagtc
    17941 attccaaaag gaagaacgaa acacggttat gttgccaaaa cagatatgaa ccaaaaagca
    18001 ttaaagaaag cgaaaacaaa tctaaagtta aaggtaaaag atatagccag acatacgact
    18061 ggcttcaata tcagcagata taatctgaca gttatcggta tgcagaatta ctactgcata
    18121 gcaactaacg tatataacaa cctgacagaa gtaagttacg ctctcttacc gaccatacga
    18181 atccgtttaa ggaacattgc aaaatcagtt ccatttgaaa gtacaagttc agaatttcag
    18241 agcaggacaa aaggaatcag accaaaaaca aaaatagtga tgattgcgga taatccgcta
    18301 ttgccgattc aaggagtaca acacaaaaat cctatgaatt tctcgcaaga tatttgtaat
    18361 tttacaaagc aagggagaaa caaggtgcat gaggacgttg tagttgtaac aaaagaagaa
    18421 atccgagcgt tgttggaaaa tgaaaaccct gccgatagcg tagagtttaa cgacaatcgt
    18481 atttcggcat atatcgcaca acaaggcaac tgctatgtaa tgaatcgtcg aggaactccg
    18541 tccactctga tatgtattca caaatcagac aaaggagata accttgacag atattctaat
    18601 ctggcatttg tggaaattcc cattgcaaag gcaatcctaa cagaatcagc agacgaagca
    18661 aaatcactgt taaaaggtta tgttcttaat agtcagcaga agaaaaaact gaacagaata
    18721 cggacgaatt acggatacca gacaataact gggaaataac ttataaatct atttggatat
    18781 gatggaacgc cgtatgaagc gaaagtttca tgtacggtgt gaagcggggg aaaacttgga
    18841 gataacatca aaaagttacc tatcgctata gcgaggaggg tggaaagaaa ccttgccaga
    18901 aattgcggat gaaatcagta tcctcaactt gacgagtaag tcagaaaatc aaggtctgct
    18961 tgatccttat gtgattatga agaaaaagaa agactcggaa agtttagcaa ttgatattct
    19021 gactttttta acaggaattt caagtcgaga cggggagaag tttcctgtgt tacgtaaagc
    19081 cattcgaacg gtgactcaaa gtgatcaacg ggggttatta caagttatta acgaattgcg
    19141 aaaagacggg tcaccaatcg cggaaaatat cgccgatcat atcgagtcct ttaccgatta
    19201 tgattttgct catttgcttt tctcagacgg ctcgattacc caatcgatta gtttagataa
    19261 acagctaaat atcattcaag tggcggattt ggttttaccg gatgcagaaa cgacttttga
    19321 agaatatacc accatggaat tgcttagtgt ttcgatgcta atcgtcattt ccacttttgc
    19381 cttggacttt atccatagta accgagaaat cttcaaaatt gtcgatttgg atgaggcttg
    19441 gagttttctt caagtggctc aaggaaaaac gttatcgaat aagcttgttc gtgctggtcg
    19501 ggccatgaat gctggggtct atttcgtcac tcaaaatagc gacgatttat tagatgaacg
    19561 tttaaaaaat aatatcgggt tgaagtttgc ttttcgcagt accgatattc atgaaatcaa
    19621 gaaaaccttg gaattctttg gtttggatca ggaggatcaa agcaatcaaa aacgcctgcg
    19681 agatttggaa aacg
//
LOCUS       DAFGZE010000052        19436 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.054, whole genome shotgun sequence.
ACCESSION   DAFGZE010000052 DAFGZE010000000
VERSION     DAFGZE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.054"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(155..1606)
                     /locus_tag="LTU54_001981"
     CDS             complement(155..1606)
                     /locus_tag="LTU54_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBL8296688.1"
                     /translation="MYKKSSRGWVKHIDFIILDLLCIQLAFYLSYVLRQVDWNPYVIP
                     MYQNMAVFIELADLLVIFMFEAYRGVLKRGYYIEFASSVKQAIMLVLLGSLYLISVQG
                     GNDYSRVVLFLMGGLYVVITYITRLLWKHYLKHKMKNEGNKSLIIVTSSSIAEVVIRN
                     VKEHNYKMYKINGVIIIDKSMIGEDVEGVTVVSDSKNAADYLCQGWVDEVFIILNKST
                     SCMKEFIERCSEMGLTVHLNLAKVSDSALGKQSIGRIGEYTVLTTSINVMTTQQAFIK
                     RTIDILAGIVGCIATGIIFIFIAPLIYINSPGPIFFAQERIGQNGKPFKMYKFRSMYM
                     DAEERKVELMKENKMSNNLMFKMEFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FFNVLTGKMSLIGFRPCLKSEYEEYDYHHRARIAIKPGITGMWQVSGRSDITDFEEVA
                     KLDTEYIKNWSMGLDFKILFKTVKTVLQRGGSM"
     gene            complement(1927..2691)
                     /locus_tag="LTU54_001982"
     CDS             complement(1927..2691)
                     /locus_tag="LTU54_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HBL8296689.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKTPVIVHPERNVHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(2719..3417)
                     /locus_tag="LTU54_001983"
     CDS             complement(2719..3417)
                     /locus_tag="LTU54_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HBL8296690.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGSVADVIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILSEARQLFDV
                     VIFDMPPVVAVTDAQIMSLKTDGTLLVVRENMSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(3429..4208)
                     /locus_tag="LTU54_001984"
     CDS             complement(3429..4208)
                     /locus_tag="LTU54_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HBL8296691.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGTAPARPKATNDKTAPSRRSRSRV"
     gene            complement(4224..5168)
                     /locus_tag="LTU54_001985"
     CDS             complement(4224..5168)
                     /locus_tag="LTU54_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL8296692.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(5214..5993)
                     /locus_tag="LTU54_001986"
     CDS             complement(5214..5993)
                     /locus_tag="LTU54_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296693.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHLNNQRQYHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNKLFTKNVSNWQQAVDDVIIKEKLA
                     SADVAHVRENMSFFKDSAWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKIRAAYATKAQTVAQQMGYSNISY"
     gene            complement(6373..8448)
                     /locus_tag="LTU54_001987"
     CDS             complement(6373..8448)
                     /locus_tag="LTU54_001987"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBL8296694.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLAGVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLEDVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(8450..9367)
                     /gene="glyQ"
                     /locus_tag="LTU54_001988"
     CDS             complement(8450..9367)
                     /gene="glyQ"
                     /locus_tag="LTU54_001988"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBL8296695.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPETVKEAE"
     gene            complement(9753..10571)
                     /gene="recO"
                     /locus_tag="LTU54_001989"
     CDS             complement(9753..10571)
                     /gene="recO"
                     /locus_tag="LTU54_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBL8296696.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKEIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFINQMKSWAHVLKTSEKDMSEKEKETPEKEE"
     gene            complement(10714..11613)
                     /gene="era"
                     /locus_tag="LTU54_001990"
     CDS             complement(10714..11613)
                     /gene="era"
                     /locus_tag="LTU54_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBL8296697.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(11628..12023)
                     /locus_tag="LTU54_001991"
     CDS             complement(11628..12023)
                     /locus_tag="LTU54_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HBL8296698.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFTVIVGLILFVPKIYHLLFN"
     gene            complement(12007..12483)
                     /gene="ybeY"
                     /locus_tag="LTU54_001992"
     CDS             complement(12007..12483)
                     /gene="ybeY"
                     /locus_tag="LTU54_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBL8296699.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(12512..14704)
                     /locus_tag="LTU54_001993"
     CDS             complement(12512..14704)
                     /locus_tag="LTU54_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBL8296700.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKAIDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNGKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(14728..15699)
                     /locus_tag="LTU54_001994"
     CDS             complement(14728..15699)
                     /locus_tag="LTU54_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBL8296701.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            16404..17783
                     /gene="brnQ"
                     /locus_tag="LTU54_001995"
     CDS             16404..17783
                     /gene="brnQ"
                     /locus_tag="LTU54_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBL8296702.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            18065..19405
                     /locus_tag="LTU54_001996"
     CDS             18065..19405
                     /locus_tag="LTU54_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL8296703.1"
                     /translation="MSYSEFTKELLDILDLNLIFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTILRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPLSMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKRDRRS
                     KHLKKYWRLLQKNAWELKGQHRYWRPSFRDHLTEAEIVDRLLSYDDSLKRGYEVYQDF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYQERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT         5377 a         4114 c         3450 g         6495 t
ORIGIN      
        1 catatttaat tcataaatat aaatcttcat tttaaatgta tgcccaaaaa tttatctgta
       61 atgcgtcatc aaaatctaga ttccaaatct ttatcaattt ttatccactc attctaccac
      121 tttagtaata ttttctattt ataacacttt cgtcctacat tgatcccccc ctctgaagta
      181 cagtttttac tgtcttaaat aaaattttaa aatctaaccc catactccaa ttttttatat
      241 attccgtgtc aagttttgct acttcttcaa aatcagtaat atcacttcta ccacttacct
      301 gccacattcc agtaattcct ggcttaatag caattcttgc acgatgatga taatcatatt
      361 cttcatactc tgatttaagg catggtcgaa atcctataag gctcatcttt cctgttagaa
      421 cgttgaaaaa ttgagggaat tcatctaaag acgtggatct aataaattga ccgattcctg
      481 tcttttttgt tccatctgga agtattttat ttccgataat tcttggatca aactccatct
      541 taaacataag attattgctc atcttatttt ctttcattaa ttctactttt ctttcttctg
      601 catccatata catacttcta aacttataca tcttgaacgg tttaccattc tgacctatac
      661 gttcttgtgc aaagaaaatt gggcctggtg aattaatata aataagtgga gcaataaaga
      721 taaaaataat tcccgttgca atacatccaa caatacctgc aagaatatca attgttcgtt
      781 ttataaatgc ctgttgagtt gtcataacat tgatacttgt tgtaagtact gtatattctc
      841 ctatacgacc aatagattgt tttcccaaag cactatctga tacttttgca agattcaaat
      901 gtacagtaag gcccatttca gagcatcgtt caataaattc tttcatacac gaagtacttt
      961 tattcaaaat aataaagact tcatctaccc atccctgaca taagtaatcc gctgcatttt
     1021 tggaatcaga aacaactgtc actccctcaa catcttcacc aatcatgctt ttatctatga
     1081 taatgacacc atttatttta tacattttat agttatgctc tttcacatta cgaataacta
     1141 cttcagcaat actactagat gtaactataa taagtgattt gttcccctca tttttcattt
     1201 tatgtttcaa gtaatgtttc cataagaggc gagttatata tgtaattaca acgtataaac
     1261 ctcccatcaa gaaaagaacg actctagaat aatcatttcc accctgaaca ctaatgagat
     1321 acaaacttcc taacagcacc aacataatcg cttgctttac agaagaagca aattctatat
     1381 agtaccctct ttttaataca cctctatatg cttcaaacat gaaaataact aaaagatctg
     1441 ccagttcaat gaagactgcc atgttttgat acattggaat cacataagga ttccaatcta
     1501 cctggcgtaa gacatacgat agataaaaag caagctggat acacaataaa tctaaaataa
     1561 taaaatcaat atgtttaacc catccccttg aacttttctt gtacattcct ttttactccc
     1621 ctctttttaa taaaataaat tgtattactt gttttctctc ctaaatatct ttctgaagga
     1681 ttattgactc gacaaattgg cttgtggata ttcttatata tagaacatcc gtaagccaac
     1741 ttaaaactcc ttaacaaaat ttttttactc atattgtaga tcaaaacaag tatccaagaa
     1801 atcatatttt atttcttttt tctgcttact caacaacagc taaaagaaaa atcatcataa
     1861 acaaattcaa ctaagccaac gatccccttg tgaaatcaag ttttttcttg atttacgaat
     1921 aaagagctaa aataaaccaa acttcttctt ttttacctct tcatagactg gataactgat
     1981 ttcatccccg ttgatcagat cctttgcgac ttgtttcatc tgtactactt tttccttacc
     2041 aaagtccttt gcaatctgct catagcactc ttttaagtaa aaggtgcgct tagagacccc
     2101 atgggcatca gaagcagcca tctgcacgag attatgtttg accatcgttt tggctgtttt
     2161 ctggatgtct ttgccaaata ccccaacata acttggcgca gtgagttggg ccaaacatcc
     2221 catatctagg aacggaagca aatgatttgg gtcttcgcga aaatgcacgt tgcgttctgg
     2281 atggacgatt actggtgtct ttcctagttc taacaactca aaaaaaagcc gttctgcata
     2341 taacggcact tccagtgtcg gaaattcgat cagaagatat gtatcatcta aatctgtaaa
     2401 taatatttca tctcggtgga tgtcttcgat gagcgttccg gtaatacgta cttcttgtcc
     2461 ttccaataac gtcaaaggca actgtctttt ctctaattcg gcttgtaacg aagcgactag
     2521 agaaatgact tgtgattttt cattgctgta ttttccatta ttgtggtgtg gtgtgcataa
     2581 aatatgcgtg atgccttgct gaatcgcttt ttcagccatt gcaatacttg cttctaagtt
     2641 ttctgccccg tcatcgattc ctggtaggat atggcaatgt agatcgatca tttctcttgt
     2701 ctcctctctt cttattcctt aatttccgta gtaataacca gcatctttgc tatgttctgc
     2761 tccgttgtac acaacaccta agactcgtgc ttgtaccatc tccagcagtt cttttgcttt
     2821 agtcaaggat tctttacgtg acatattttc acgtacgacc aataaagtgc catccgtttt
     2881 taaagacatg atctgtgcat ctgttacggc tactactggt ggcatatcga atatcaccac
     2941 gtcaaataac tgtcttgctt cggataggat ctgatccatt cttggcgagc ttaacaattc
     3001 tgatgggttg ggtggcttag gtccactagg caaaatcgac aagttctcga ctggcgttcg
     3061 ttggatcaca tctgctacac ttcctgatga actaagtgct gtacttaatc cactggcatt
     3121 gttcagctgg aatgtcttat agacgacggg tttacgtaaa tctgcatcga ctagtagaac
     3181 tcgttgacct gatttagcaa aaacgactgc aatgtttgct gcagtggtgg atttcccttc
     3241 tccaggacct gatgaagtca cgacaatcgt tttgatttgc tgaccagctg cggaggcaaa
     3301 ctggatattc gtccggatcg tacgatattg ttcggcgatt ggtgaagatg ggtttgttaa
     3361 tgtgatcaaa ctgactgcat gagtttgtgc tgctttttgt ttctgtgttc gtgccatttt
     3421 tcttcctcct agactcttga gcggcttctg cgtgatgggg ctgttttatc gtttgtagct
     3481 tttggtcgtg caggagctgt tcctttttta cggataccag cgttcaattc tttcgctgac
     3541 atttctggaa ctgttcctaa gattgggaat cctaatgtat ctgtcacata tttttcatct
     3601 ttcactgtcc gatcgagtaa ttccaataag aatgccagac ctacacctac catcattcct
     3661 aacattaagc cagtcattaa attcaattta ttatttggcg aaacaggtgt cgtattggca
     3721 actgcacctg aaatgatcga gattttatca acattcatca catcttttgc gttctcttgg
     3781 aaaacttgtg aagttgtgtt agcgatctgt tgagcagcca ctgcattcgt acttattgcc
     3841 tgaatcgaga acatttgtga attttctgcc tgattaactg agatggcatc tttgatttgt
     3901 tcagcagtca ttttcacatt gtcttctgtt tctaaacgat ctttgacttc attgagcact
     3961 aaatcactga cgatcatatc tttatacgta ttgatcattt gcaggttcgt attgacatca
     4021 ttgacatttg cagtgtctga ttgtggcagt gtcacgatca attgtgcttg tgaactgtat
     4081 tttggtgtca agatgaaaaa tgttacgatt cctgagatgg ccaagccagc aaacattgag
     4141 atgatgatta ttaccaagtg tttgcgtaat gtttcaaaaa tttctttcaa gctgattgtc
     4201 tcttccattt tttctccact ttcctattta ttctctagat tcatttgatc ttttaattct
     4261 tttttcacac gatcaagttc ttgatcactg actttttgat aggagatatt atcttgcatg
     4321 aagccttctc cttgcagctg atcttgctta atcgtaccaa atgcctcccg ataatctaaa
     4381 gcaatttttt tcatttcatc aaagttcatg tctgttttca tattcgactc tagagcattc
     4441 aaaacggatt gatagttgct gacaccgttt agactcaata cttttttcgc tacgccttct
     4501 acgatctttc tttggcgccc ttgtctaccg taatcaccat ttgggtcttc ataacgcata
     4561 cgagaataag ataatgcctg atccccgttc aatgtaattt taccgattgg aaagtcatag
     4621 ccatcttgtg agaaagtgag gtcattattg acttcgatac caccaactgc atcaacgagt
     4681 tcctttagtc ctttcatatt gatagagaca tagtgatcga ttgggatgtc tacatatttt
     4741 tgtacggtat ccatcgccat ggcagctccg ccgaatgcgt aagcgtggtt gatcttgtct
     4801 tttgtgtcac gacctacgat gtcgacatac gtatctcgag gaatgctgac gatcgttgtc
     4861 tgtttgtctt caggactgac tgtggcaacc atcatcgtat ctgatcttcc ttgatcgacc
     4921 cgacctaaat ctccagtatc aatccccatc aatagaacag agaaagattc ttgtttgtcc
     4981 agatcaactg gtttcgttcg ttctccttgg gttcgttcca ctgattcgta agttttctca
     5041 atagaatgtg agagatccag atataatttt gctcctactc ctacaattgc taatactaat
     5101 acgataagaa ctgctaaaat aatcaaaaat atttttttcc ctcttgattt tcttcttcgt
     5161 cttgccatag tttcctccgt ttgctaacct tgcttgaatc aacgttttaa ctgtcaatag
     5221 cttatattgc tatagcccat ttgctgagca acagtttgtg cttttgttgc gtatgcggct
     5281 cttatttttt cattccggat ctgctctatt tgactgagcg ccgtcaaata ttgatcgtaa
     5341 gtagcggtag ctgttacttg accgtctttc agcatcgtat cgaatagctg atctagttgc
     5401 gtgacttgtg caatttgggt atcagcgaat cctagatatt gcataacgac tgttttccaa
     5461 gcactatcct taaaaaagct catattttca cgtacatggg cgacatctgc acttgccaac
     5521 ttttctttga tgatgacatc atccacagct tgctgccagt ttgatacgtt tttagtaaat
     5581 aatttattga cagccgtctg gatcttcagt tttgattctg catcctctaa ctgcgttaag
     5641 actgctttct tttcttcttg gatcgcagca gcttctttag gtaattctga ttccttgatg
     5701 tcataatcat ctgccgaaag tttgatagaa gatagtttat gtgacaaatt tgaaactgtt
     5761 tcttctgtta tttcaggatt taggaaaatt ttttcttttg tactgtacag cttatctact
     5821 tcttttgata aagaggtcac ggtgtctttt tgattggtga tggcagattc tgcgtaattg
     5881 atccgttgat gatattgccg ctgattattg agatggtatc caaacaagca aatcacaata
     5941 gcgcctgtgc ctaccgccac agtagccaat aacttgacga acggcttttc catagattca
     6001 ctcctaaata ttgatgatag ataagtaaag atgtgattca ttcacatcag aatgagcgct
     6061 ttttatttta aaagcataat taaactaata tattcataga aatatttata aacaggtttt
     6121 ccctatataa tcataccaca tacagttttt ttgactaggg aaattcctaa tttaaaagta
     6181 aataaaacag taacaagatt ataattaatc gttttcattt ttatttcctc acttttcatg
     6241 agcgttttga aaattcgaac aaagaataaa ataacaaaaa ttattaaaat agaattaata
     6301 acagctattt ataacaaaaa aaacagagac acagcacgtt tcgtgcatgc tctctgtttt
     6361 tcaaacaaaa gactattttg tgatgatttg atccaagctt gctaatgaca aagccatggc
     6421 agctatttga cttagttgtg ttagtcggtt cttacgtact gctggttcat cagccatgac
     6481 catcgtttga tcaaaataat tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt
     6541 ttcgtggatc gtttggtcag caaaacttgc tttcattgct tctactgctt tgtgtaattc
     6601 tttctctgcg tcgttttcga acagctcagg gtctacttct gattgttttt caagctgcgc
     6661 ttttttcgct aagttcacga cacgtgtcaa agcttccatt gaaggtttga agtcttcatc
     6721 tgcaagatgt tgtttgaaca attgtgctgc tgcaaatact tttgtcagat ctttttgttc
     6781 cgcattcaaa acagcatcga tgacatcatg acgaatattc ttcgttgtca ataattggcg
     6841 cagacggcct ttgataaagt cgatcacttg ttgttgccct tctgataaac caatgccgaa
     6901 acgatccgta tctttactga taacatcgct aatcgtttct tgcagaacag acaaaggaaa
     6961 ggcccatttt ttcgattcga tgatccgaac gacaccgtat gcttggcgac gtaaggcata
     7021 aggatcgttt gacccgcttg gaatcatccc aactgcaaag aaagaaaaga tgctatctaa
     7081 tttatctgca atcgctaaaa cagaacctac tgtagactga ggtagctctc cttcactgga
     7141 tataggcatg taatgctcac gaatcgctgt cgctacagca ggtttctcgc ctttcagaag
     7201 agcatatttt tcgcccatga tcccttgtag ttcaggaaat tcccctacca tgttggttac
     7261 tagatcaaat ttatagattt cagcagctcg ttgcaaatcc atcaattctt cctcagatag
     7321 tcctacttgt tcgccaatga tacgggcaat gacttgtacc cgttgcattt tctcatagat
     7381 actaccgatt ttttcatgga aagtaacagt ttttagtttt tcaacgtatt ggtcgattga
     7441 agctttcttg tcttcattat aaaagaattc cgcatcttct aaacgagccg tcaatacttt
     7501 ttgattgcct cgaaccacat cttccaattt tacccgatca ccattacgag cagaaataaa
     7561 atgaggaagc agcatacctt gatcattccg tacctcgaaa taacgttgat gttctttcat
     7621 agaagtgact agtacttctt caggaacaga aagatatttt tcttcgaagt cgccagaaaa
     7681 gacagttgga tattcgacta aattgttgac ttcttctagt aaatcttcgt ctagatcaat
     7741 aacccagtga tttttttctg ctaattcgtt tgcttgtgta acgatcattt cttgtctttt
     7801 ttgcggagac gcaatgacga attgttcatt caatttttct tcatattctt ttgcatgcgc
     7861 aaatgtcaca tcttcgccta agaaacgatg acctcgagag gctctgcctg ttttgacatc
     7921 caagatttcg aatgggatta cttcgtcatc taacaatgcc acgatccagt gaatagggcg
     7981 gatatattca aaatcgtatt ctgcccaatg catcgttaca gggaaagtca aacttttgat
     8041 cacccctgct aagcctgtca atacttcttt tgcttcctta ccatgagtgt atttcgttac
     8101 atagacatat tccacacctt ttaattctct aaaagtgata gcgtctgtcg tcagaccttg
     8161 tccgcggaca aatccttccg ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt
     8221 tttggctggt cctttgactt cttcttcact gtccgcttgt cgttctggaa tatttgttac
     8281 tttgattgct aaacggcgcg gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt
     8341 ttcatctaaa aattttgctg ttttttcttc taattgtttc atgcttggtg tgacaacatg
     8401 ggcaggcatt tcttccagcc cgatttcaag taataagtct tttgccatct tattcagcct
     8461 ccttcacggt ttctggattt gctgtgtttt tattcaacaa agggaatcct aatttttcgc
     8521 gttctgccac aaagattttg gctacagcac gagccatgtt gcggatgcga gaaagataac
     8581 cggcacgctc tgttacggat actgcaccgc gggcgtctaa taagttgaat gtatggctac
     8641 atttcaagat ataatcatac gccggatgaa caagattttc atcgatacag cgtttagctt
     8701 ctttttcaaa acgatcaaag ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat
     8761 atttggaatg ttcatattct ggttgtttga agatctcgcc atatttcacg ccatcagccc
     8821 attctaaatc atagacactt tcaacttctt ggatataaga agcaagacgt tctaatccat
     8881 aagtgatctc agaagttact ggtttacaag gcagtccacc aacttgctgg aagtacgtga
     8941 attgtgtgat ttccattccg tcaagccata cttcccaacc cacaccggca cagcccatgg
     9001 atgggttctc ccagttgtct tcaacaaaac ggatgtcgtg ttctaatgga tcgattccta
     9061 aaaggcgcaa gctttctaaa taaagctctt gaatattctc aggtgaaggt ttcatcacca
     9121 cttggaattg atgatgctga tacaaacggt ttgggttttc accataacgt ccatcagctg
     9181 gtcggcgaga tggctctacg taagcagcat tccatggttc tggtccgatt gcccgtaaaa
     9241 aggtatatgg gctcatcgta cctgctcctt tttctgtgtc ataagcttgc atcagcatgc
     9301 agccgtttga tgaccaaaat ttttgtaatg ttaaaatcat atcttggacg gtcaattttt
     9361 tactcatctg ttttgcccct ttcaatttta aagcgttgct gtcaaataag aatacaggaa
     9421 agaaaacgac aaaaaatccc tatgtctgat tacagacata gggacgaata attcgcggtt
     9481 ccaccctact tccgaataaa gattcggcag cttcatttat cgtactcgca agtgcctctt
     9541 caaaaatcgt ttgggtccgg ctcccactct cccgaactcg ctaaaccacg tgttttttac
     9601 tgatcttggt caatgtacgt attcattttg cagttttctt gtttctataa tagatcctat
     9661 cactgaacga tctatttaat ctatggttaa taatagacgg aactccctgt gtttgtcaaa
     9721 tggttttgat tttttgttgt atgttttatt tactattctt ctttttcagg tgtctctttt
     9781 tctttttcag acatgtcttt ctctgacgtc tttaatacgt gtgcccaaga cttcatttga
     9841 ttgatgaact ttttgctttt caaatggata ccgacatatt catcatacag ctggtcgatg
     9901 agttcacgga ttgcttgttt ggtttcttct ttcagatgga ttccactaat tttatcataa
     9961 gaaatagctg aaaacatccg aacgaaataa acagctcgag gatctgcatg ataacggtga
    10021 tcgtccatcg accaatgtct ttcgcatatc acaccaccat atttggaaga atagtcaaat
    10081 tttccttttg tttccccgca tacgacgcag tgtgtccaga ttggtgcaat tccgaagcgt
    10141 tgcagtattt ggacttcaaa aatattcgtg atgatttctg catcgtctcc ttggtccatc
    10201 cgttgcagtg cttgttgcgt aaactggaac aaatgcgggt cgtactgatg atcttcgatt
    10261 gccgcatcta ctagattcaa taaatatgtg ccatagccgc tcaaaaagat gtcttcttgg
    10321 atgtgtcgaa acgcatggac atctttgaca ctgcttaaaa atgacaaacc ttcacttcga
    10381 aaattcccga tataaactgc ttcagtaaaa gggagcaaag ccggagctaa gggctgattt
    10441 tgccgatgga tctctttgat ataaaacatc attttgccag aagattctgt aaagattttg
    10501 actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt tgattctgca
    10561 agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc tgccgatcca
    10621 gatcatttat gttgcgatct tccgcagctt ttctcaacac gcggattttt attcagttta
    10681 tttctatacc taatttacaa gaagctcaga atatcagtat tcgtcttttc ggtagccgaa
    10741 gtcttgcaga tagacttttt tgtctcgcca gtctttttga actttgaccc aaagttctag
    10801 aaagactttg tctcctaaaa gattttcgat atcttttctt gctttcgttc ctacttgctt
    10861 cagcattttt ccgcctttgc cgataatgat ccctttttgg ctatctcttt cgacaataat
    10921 cgtagcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa caacgactgc
    10981 gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga tcaattcaga
    11041 aacgatgaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt gtgggccctc
    11101 tggcatttca ctgactaata catccatcaa acgttcgacg ttgtttcctt ctgtagcaga
    11161 gattggaatc acttgtgcaa aaggcatctg cgttgaataa tcttcgatga ttcccaacaa
    11221 ctcgtctgga tggattttgt cgattttatt gataatcaga taaacaggag aatgcacgtt
    11281 ttttagacgt tcaataataa aatcgtctcc ccgaccgcgt ttttgatcag cactaatcat
    11341 aaataagacc gcgtccactt ctttcaacgc actgtaagct gcttctacca taaaatctcc
    11401 taaacgatgc tttggtttat ggattcctgg tgtatcaata aaaatcaact gagcttctgg
    11461 tgtagtgtat actccttgga ttttatttct agttgtctgt gccttgtcac tcatgatggc
    11521 aattttttga cccacgattc gattcaataa tgtggatttc cctacatttg ggcggccgac
    11581 gattgcgaca aaccctgatt tatgttctgg catatatgtt ctccttttta attaaacaac
    11641 aaatgatata ttttagggac aaataaaatc aacccaacga tgacggtaaa acaagcggtc
    11701 actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg aaaatgaaaa
    11761 tccgtaaaca tgtccacgac attttcaaaa acagtattga tgatctctac tatccatacg
    11821 agaaaaacag ctaaaagcag ccataaccat tctatccgat caagttggaa aatcacgccc
    11881 ataaacaaag cgaggattcc gaacgcaaca tgtttcctca tgttgcgttc ttcctcaaaa
    11941 acagttttga ctccttggac ggcaaattcc acggacatca ggaaatgttt gtttttctct
    12001 acgtttttat cttttaagtc cataggcatc taagatctct ttctgtaaac caaacatttc
    12061 tttctcatcc tctggtgtca tatggtcata cccgttgata tgcaagaatc catgaaccgc
    12121 taaaaagcca agttcccgat catacgaatg gccgtaagct tctgcttgct ctgcagcacg
    12181 ttcagtagag atcatgatgt ctcccagtac acgaggcaat ggattctctc catcttcaaa
    12241 aatcactggg atttcgtctt cgccctcttc ttccaaggca aaactgatga cgtctgtcgg
    12301 tgcatctttt ccacggtaat cacgattgat tacttggatt gctgcattgt ccatgaaagt
    12361 aacagacatt tctgtttctt ctggcaactc taaatatgat gctgcaaatt gcagcaagtc
    12421 gtcgatttct ttcattttct cttctgagac ttggtttgtt tcatccatga aggtaatatc
    12481 cattatacat cgcttctttc tattcttctt gctattcttc ttgttcttct tttatcttct
    12541 caacttccga tttcatttta ggatatttga tccgagaatg gaaggtactg cttaaaccgc
    12601 tgatcaatga tttttctact ttacggattt cttttaacgt caatccactg tcgtccaatt
    12661 gtccatcgga aatacgctct tcaataagat tatggacaaa actttcgatt ttttcgctcg
    12721 ttggatggtc cattgcccgt actgccgctt cacaactgtc agctatattg acgattcctg
    12781 cttcacggct ttgaggtttt ggtccaggat aacggaattg ctcttccgta acttccgggt
    12841 tgcgttcctt tgccttaacg tagaaataac gcatcagcgt ggttccatga tgctgtctac
    12901 aaatatcgat caccatttgc ggcatattat aatcttccaa tattttggca ccatctgtca
    12961 catgtccaaa gataatatgt ttactgtctt ctggcaataa gaaattgtgc ggattttccg
    13021 ctcctgaagg caagttctca acgaaaaagc tggcgtgttt gatcttgccg atatcatgat
    13081 aatagcaagc tactcgagtt agtaaagaac gtccaccgat ttctgctaca gcattagcac
    13141 ttagattggc taccatcatg ctatggtgat acgtacctgg tgcttcttcc agcaactgtt
    13201 tcaataaagg atgattggga ttgctgagtt catttaatac gatcacacta tcatcattga
    13261 ccatcaattc aatatagggg tgcagaccta tcgtcagcaa gaatgaaaac agtgcacctg
    13321 tgaatccgca gactagcgtt aaccaagtcg ttgcatcccc aaagctcatt ccttggtaga
    13381 taatcaaaat gacatccatc atcacaggaa aaataatgac ccacatagca gcggataacc
    13441 cttgttctga gatccgtttg cgtctgacga tcgttgccat gagaccagaa aatacatagg
    13501 acatcaaaat aactgtcaga aagttcgtgc caatcgaatc ataaaaaatg aaaagtgcgg
    13561 atactgcttg aaaaatacca gccatgatcc ctgctcttcg attcaagaaa aagcttaaaa
    13621 caagtggagc aaaagccgcg gggtaaaaca gcggaatata agcagcttgt tctgtttgga
    13681 agagttggaa aaatttcatt agtacgacgc ttactgacat tgccgtcacg tagaaaagta
    13741 cgtattttgt tctttctcct gcattttgga attgaagtga attataaatc agtacaccga
    13801 tttgaagcaa aacagctaaa accatggcaa tgagtgggaa aatagaagtc gtttgattcg
    13861 tcaatcctag taattccaat tttttcatcg ctgaagcatc aatttgattt ccttcgcgaa
    13921 caataatttc tccttggaag atcatcactg gttgtacagc ttctcgtgca gattgtttta
    13981 attcttctgt ctttttctca ttcaatacat cattaactac gatgccttga tccactaagt
    14041 agcgaatcat ttgttgctgc tctgttgtga cattcaaata ttgaatctga tcttctgctt
    14101 cttgtttaaa agcttccaat tcgctttcac gaacttgttt actcatctgt tcgtcaatga
    14161 gcttcaaact ttcatcccta actgtgtgaa tttccgtttc agtcatttca aatatcgtcg
    14221 aataaaaatt gttcggcagt ttttggtaaa acgcaacgtt ttctgcattc acgttttcaa
    14281 aatttttctt cagtgtagca atccgctctt ctgtagtagg tttcgggata ttttcatctt
    14341 ccttcgcctt atctaccttg ccattataag atttgttgat ggtatcattc gtcttatcga
    14401 tcaattcgaa taattgcttg atccgattgt tctggtcatc tgccagatct ttttgataag
    14461 tatattctgg agtcactgct tctgctgcca gttttcttct ttgttcggtt tcttccgtgt
    14521 tctcaatcgt tttattcgca cggatactct cttctgctac ttggccttct ctgaaatcaa
    14581 tggccttttg tttgacacta ctaaatgtaa cgccgcagac aatgataaaa aacaagaaca
    14641 gaaggaaagg caatgctgcc tttccgagtt tttcttgtag tttctgcagt gttttattca
    14701 acatgtgctt atcctcctaa gcaaagctta ttcttgatga agatccgcct tttcataagc
    14761 tttgatgatt tcggcaacta cagggtgccg aacaacgtca cttgcttcaa aattaacaaa
    14821 atcgattttt ttgatttgct ttaatgttcg ttctgcatga acaagaccac ttgtcgtccc
    14881 acgaggcaag tctatctgac ttgtatcccc attgacaatc atttttgatt gataaccgag
    14941 acgggtcaag aacattttca tctgagcgac tgtcgtgttt tgtgcttcgt ccaagataac
    15001 gaatgcatca tccaatgtcc gcccccgcat gtaagctaaa ggtgcgattt cgatcacgcc
    15061 tcgttccatc aatcggttcg tatgatccaa tccaaaaatt tggtataaag catcataaac
    15121 aggccgcaga taaggatcca ctttttcttt cagatccccg ggtagaaagc ctaggttttc
    15181 ccctgcctcc acagcgggcc gcgtcaaaat gattttttgc acttcgcctt tttttagggc
    15241 agcaattgct aatactacgg caagaaacgt tttccctgtt ccggctggcc ctacaccaaa
    15301 tacgatgtca tgtttggcta ccgattcaac gtatttcttt tgaccgctgt tttttacccg
    15361 tataggtttt ccattacggt ctttgacgat ctcttcctca tacatctcaa caaaataatc
    15421 taatgtacct ttttgcccca ttttcaatgc cgtcacaaca tctggcgtac ttacgtggat
    15481 tcctcgtttg atcaattcct gtaatgtccg aatgactgag gcagccaagg aaatctggga
    15541 ttgctcacca atgatctgaa tgatttctcc acgtgtatgg atggttgttt ccgttgtttc
    15601 ctcgatcagt tttatatgct tgtcgtgtgc acctaaaagc atgctgacat catcagcagc
    15661 tgtcaatcta atctctaaag aactagactc ttccgtcaaa aatagacatc cccttttttt
    15721 cgattcattc tttcctattt taccatactc agaagaaact atatagcaaa taagaaaagg
    15781 ttgtaaaaga gtcgcttaac catacgtacc aagaaacgtt aaaaaatagc cgtttccatt
    15841 cgctaaaggt ctcgttttga taaaaagaag ctgagaatgt aagttatatg aaagtggaga
    15901 acaaaatatt tcgtttcatt caacaagtat tctctaggta tttttcctgt ttcaaaaaaa
    15961 gaaaacaaaa tagttaaaat attcttgcat ttcaaaaaaa acaggtgtat tattacgtca
    16021 acaaataaac atacagcaaa gcgtcgagga aaacagtagg ctattcaacg atttattccc
    16081 agtgagtcat gtaagtgaga aatgacaata aatcgaatag acgaacatca tttccgagtc
    16141 aatgtcgaaa acgtagatca ttgtggctgc ctccattatc aggctagcgg ataactttat
    16201 caaaaagcgt ccgtgaatga agaagctttt ttaagcgaaa aaaggtggta ccgttctaga
    16261 atctagagcc cttttcccaa gtggaaaagg gctttttttt attttaaggt ggtggttttc
    16321 atggaatctg atattagtgg cattactaat tgctgcaacg tttatatcgt caatcaaaat
    16381 gataattatt ggaggaaaaa tttatggaag aaacaaaaac ttattcatgg aaacaactat
    16441 tgatggtcag ttcacttatc ttcgggatgt tcttcgggtc aggtaatcta atctttcctg
    16501 tccacttagg tcaaatggct ggcgcaaact gggtcagtgc tggcgtagga tttgctatct
    16561 caggtgctct tttcccatta ctagctatct tagctgtagt agtgacaaaa agtgatggtc
    16621 tgtatgactt agctaaacct gtcggaaaat ggtatgctgc cttgttctta atccttgttc
    16681 atttgacgat cggaccattt ttcggtacac cacggacagc tgctactgct tttgaaatgg
    16741 ctgctaaacc atttttgcct gaaaaatata attcattagg tatgttttta ttctctgctc
    16801 ttttcttttt acttgcttat ttccttactg tccatccaac acggttggtc aaatacgtcg
    16861 gaaagtatct aaactccttg tttcttgcat tattagcaat catttttgtc gtttctttat
    16921 tccatccaat gggaaatcta aatcaatctg ctgcagatac ttatcaaaca aacgctttaa
    16981 caaatggtgt cctagaaggt tataacacgg ttgatgcagt agctttgctt gccttaagtg
    17041 tcacttttgt ccatgcagtc cgtgaccttg gtttcaaaga taaaaaagtc acagaattaa
    17101 cggcaaaagc tggtagcctg agcgttgcgc tagaagtcat gatctatttt ggacttgttc
    17161 ttctcggtgc gatgagtttg aataagatgt ctttatcaga aaatggcgga actgcacttt
    17221 ctcagatcac taatttttat ttaggtaact tcggcgctag cttcttagga gtgatggtca
    17281 cattaggtgt cttcactacc gctatgggac tcgttgtttc ttttgcgcaa gatttccata
    17341 aactgttccc taaagtcagt tatatgacat ggctgcggtt aacgactttc gtctcttttg
    17401 tcgttgcaaa tgctggttta gacaatatca tccaatggtc tttaccagta ttgatgcttt
    17461 tgtatccatt gtcgcttgcg ttgatcttgc tttccttaac agcaaaattt ttccaaaaga
    17521 caccatttgt ctatcaagta acgatgttgt ttgctgctgt tcctgccgta ttggatatgc
    17581 tggcaaattc gcctgcttta gtgagtcaac aaagagttgt agcaagcatg ctcgaatttt
    17641 atcatcatca cgttcctttt gctgcattag gacttggctg gatggtccca acgttacttg
    17701 gttacgctgg cagcctactg ttttattacg cctatcgcct atcagggtat aaacaagaag
    17761 caaatgaact accagaagaa taaccaaatc aaacacaatt taacataaga aatattaaac
    17821 aggaaaaaac cagctagatg aaagaatcct attctttcgt ctagctattt tttagttctc
    17881 ttttttacgc gcacaaaaaa actgcggaca atgattgtcc gcagtttaaa tgggctctat
    17941 aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc taataaacgc
    18001 aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa aggagtgttt
    18061 caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc taaatcttat
    18121 ctttcatgaa gatgcctttc gcaaagagcg aatcaatgac gaaacatgtt ttgtttttga
    18181 tggcacattg acctatcaac cagaggaatg cttccattgt cactatcaaa ataagcaaac
    18241 gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca gtaactacaa
    18301 gaccattctg cggattaaca aacagcgctt caaatgtaaa cactgtggaa agactttttt
    18361 agcagaagat tcagttagtg atcgtcgctg ctccattgct cgcagagtca aacaagccat
    18421 tcttgagcta ttaagcgaac ctctttctat gtctttgatc gcacgtatga agcacatttc
    18481 tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt ctttgaacca
    18541 gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg tctcgggagc
    18601 catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg tggaaaatcg
    18661 gcaactaaat cctttacgcg actatttctt gcgctaccct agaaaagtcc gtgaacaagt
    18721 acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag agtgctttcc
    18781 gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc gggcctttac
    18841 caaccaccga attcaagtaa tgaagacctt taaaaaaagg gatcgtcggt cgaagcactt
    18901 aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaaaggtc agcatcgcta
    18961 ctggcgtccg tcatttcgag atcatctgac agaagcagag atagtcgatc ggctattatc
    19021 ttatgatgac tccttaaagc gaggctacga agtctaccag gattttcttt ccgctatcag
    19081 aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg agttgccaga
    19141 gcactaccaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca aacgagcgtt
    19201 gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca aagtactaaa
    19261 acgaattgcc tatggcttta gaaactttca aaactaccaa gaacgaatct ttttatatcg
    19321 aggaaaatat tttaagaaaa ctaagaacac tacccaactg actaaagcta gaacaacaac
    19381 acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta ttgaat
//
LOCUS       DAFGZE010000053        18994 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.055, whole genome shotgun sequence.
ACCESSION   DAFGZE010000053 DAFGZE010000000
VERSION     DAFGZE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.055"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            42..2195
                     /locus_tag="LTU54_001997"
     CDS             42..2195
                     /locus_tag="LTU54_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HBL8296704.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            2164..3579
                     /locus_tag="LTU54_001998"
     CDS             2164..3579
                     /locus_tag="LTU54_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBL8296705.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            3612..4646
                     /gene="sppA"
                     /locus_tag="LTU54_001999"
     CDS             3612..4646
                     /gene="sppA"
                     /locus_tag="LTU54_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBL8296706.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            4648..5169
                     /locus_tag="LTU54_002000"
     CDS             4648..5169
                     /locus_tag="LTU54_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HBL8296707.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            5166..5552
                     /locus_tag="LTU54_002001"
     CDS             5166..5552
                     /locus_tag="LTU54_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HBL8296708.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(6048..6120)
                     /locus_tag="LTU54_002002"
     tRNA            complement(6048..6120)
                     /locus_tag="LTU54_002002"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6085..6087),aa:Lys,seq:ctt)
     gene            6501..7070
                     /locus_tag="LTU54_002003"
     CDS             6501..7070
                     /locus_tag="LTU54_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBL8296709.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7057..7917
                     /locus_tag="LTU54_002004"
     CDS             7057..7917
                     /locus_tag="LTU54_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL8296710.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8107..8811
                     /locus_tag="LTU54_002005"
     CDS             8107..8811
                     /locus_tag="LTU54_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8296711.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8816..10651
                     /gene="walK"
                     /locus_tag="LTU54_002006"
     CDS             8816..10651
                     /gene="walK"
                     /locus_tag="LTU54_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HBL8296712.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10648..11964
                     /locus_tag="LTU54_002007"
     CDS             10648..11964
                     /locus_tag="LTU54_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296713.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11965..12834
                     /locus_tag="LTU54_002008"
     CDS             11965..12834
                     /locus_tag="LTU54_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HBL8296714.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12889..13698
                     /locus_tag="LTU54_002009"
     CDS             12889..13698
                     /locus_tag="LTU54_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL8296715.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13801..14430)
                     /locus_tag="LTU54_002010"
     CDS             complement(13801..14430)
                     /locus_tag="LTU54_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBL8296716.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14600..15892
                     /locus_tag="LTU54_002011"
     CDS             14600..15892
                     /locus_tag="LTU54_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296717.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15968..16630)
                     /locus_tag="LTU54_002012"
     CDS             complement(15968..16630)
                     /locus_tag="LTU54_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL8296718.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16855..17139
                     /gene="groES"
                     /locus_tag="LTU54_002013"
     CDS             16855..17139
                     /gene="groES"
                     /locus_tag="LTU54_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBL8296719.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            17190..18815
                     /gene="groL"
                     /locus_tag="LTU54_002014"
     CDS             17190..18815
                     /gene="groL"
                     /locus_tag="LTU54_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBL8296720.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         6463 a         3062 c         3933 g         5536 t
ORIGIN      
        1 tttttctata aaaatttttt agaaggagtt gtgttagata agtgataaaa tcatttcctt
       61 ttgtgcagca acaagatgaa aaagattgtg gtgttgcttg cctttcgatg attttaaaat
      121 attttaaaac tgatatcccg attcataaat tacgggacct atctgggact gatttagagg
      181 gtacttcagc atttggattg aaaaagacat ttgaaaaatt gtcttttgat tgtatggcta
      241 ttcaagcaga tagtgagatc tggaaagaaa gagatcttat cttaccatta attgcacata
      301 tattggttga taacagctat atgcactacg ttgtaattta taaaacgaag ggaaacgtac
      361 tatggattgc agatcccgct aatggaaaaa atagaaaaac gattgaagcg tttcaaaaag
      421 aatggacagg tattctttta ttacctatgc ctagagaaac ttatattcct attaaagaaa
      481 aagtagctgg attaagttct tttttgccta taatttggaa acaaaaagga ttagtattcc
      541 acatcgtttt agcatcgatt ttcattacgt tttttggcat aggaagctca tattattttc
      601 aaggtattct tgattttttc atccctaatc aagcacgttc aactttaaat ataatttcca
      661 ttggcttgat cgctgtctat ttatttcggg tattgtttga atatagtcgt agttatcttc
      721 ttattatttt aggacagcga atgagtatgg caattatgct tcagtatttc aaacatgtgt
      781 taactttacc aatgaatttt tttgctacga gaaaatcagg ggaaattatt tctcgttttc
      841 ttgatgccag taagattatt gatgctttag caagtgctac gttatcaata tttttagata
      901 ttggtatggt gttgcttgtt ggggttacgt tagctattca gaatagtacg ttgtttttaa
      961 tcactctatg ctctttgcct ttttatgtag ttgcaatatt cgctttcgtg aaaagctatg
     1021 aaaaatcgaa cagggaagaa atggcagccg gtgcagctgt aaattctagt atcatagaaa
     1081 gtcttaaagg aatagaaaca attaaagcat atagcggtga agagaaagta tataaccgta
     1141 tagaccatga atttgttaaa ttaatgaaaa aatcatttcg tacggttacg cttgataata
     1201 ttcaacaagg aattaaacat ttgattcaat taattagcag tgcattaatt ttatggctag
     1261 gttcttattt tgttatggat aatagaatta gtctaggtca acttatcaca tacaatgctt
     1321 tactagtatt tttgacagat cctttacaaa acattattaa tttacaagtg aaaatgcaaa
     1381 cggctcaagt tgcaaataat cgtttaaatg aaattttcgc gattgattca gaaaaatcga
     1441 accagtcttt tgaatatatg ctttctgaac aattatttca acaagatatc actgtaaaaa
     1501 acgtttcatt ctcgtatagc atgaaggccc caacgttgaa cgaaatttca tgtacaattc
     1561 cttcgcacag taaaacggct atagttggag ttagtggttc tggaaaatca actcttgcaa
     1621 agttatttgt tcgtttttat gaagcatcag aaggatcgat ccattatgga aacattaatc
     1681 acttagacat tccttttcag atattacgcg atcgagtgac atatttgcct caagagtcat
     1741 ttttctttag tggaacaatc attgagaatc ttatattcgg tttagatagc ccaccaagtt
     1801 tcgaacaaat aatagaagtt tgtgagatag tccaactaaa tgaatttatt aatcaacagc
     1861 cattacgtta cgagacattg ttggaagaag gtggaaataa tctttcagga gggcaacgac
     1921 aacgattagc cttagcacgg gctttattaa aaaatccgga catattaatt ttagatgaag
     1981 caactagtgg attagatacc ttgttagaac atgcaattat gaattatctc ctcaatttaa
     2041 aagaaaaaac gattattttt attgcgcatc atttaccaat agcaaaagca tgtgatcaaa
     2101 ttttagttct ccatgaaggg catttggtag aaaaaggaac gcacgatgaa cttcgttttg
     2161 ccgatggtat ctatcaaaaa ttatgggaaa tctaggagag ggaaacaatt tatgcagaat
     2221 aataattggt tcgaacattc tagtatctat actaaacaac atcatagctt ctaccgttgg
     2281 gtgttatacc ctgtaattct ttttttatgt attattggat tgtttttaac ctttgctaaa
     2341 aaagaggttg ttattcggac aacggctcaa ttgaaccccg aaaaaataga gaagttacaa
     2401 gtgccattag aagctaaaat aatggaaaac ttcctaaaag aaaatcaatt tgttcataag
     2461 gacgatgtgt tggttagatt ggattgttct ttaatagaga atgaaaaggc tcagatagaa
     2521 caagaaaacc aacgtattac tcaacaaata aagatggctc agctatttat tgaaagtata
     2581 agtaaaggaa aaaatttgtt ttcaacggat gacagttttg gctacagtaa tcaattaaag
     2641 agcatgttgt cagaaaaaga atcactccgc tacgctttga agcaaagtga attaaatgat
     2701 caaaagcaat tagaagtata cgaaaagaca aaaagacaac tagaaaaaca aattgagagt
     2761 tcagatagta aattacaaga atggcaacaa gtacaggtag cttggagtaa taatcaatca
     2821 ttaaaagatt tttcaaaaga aatgatggca aactatgaga attggcaaga acaactaaat
     2881 aatgtttctg atgatcaaaa aaatcaagtg aaactgacaa tttcagcaag cataaatgaa
     2941 caaattgagc aactaaaaaa agaagtagaa cagtatcagt cagaaaaagc taaattagtt
     3001 aaaccaacta cttctgagaa tgacagaatt agtcaaacgg aaaaaggaaa gcaagagcta
     3061 gaacaagcaa taacaactgt aaaacaaaca gttacagagc taaatgaaaa acaagagaag
     3121 aaccagtcag ttatcaaaag tttggacgat caactttcaa aaggaatact aaaagctcct
     3181 gtatctggaa cggttcattt aaatgaagaa acaaagggac aagcagaact agccaaagga
     3241 acagtgcttg cagaaattta tccgatccac gaaaaatcaa aaatgaaatt tacagcactt
     3301 ttacctgcga atgaaagtac ctatattaaa gaagggatga aggttcattt taaattggat
     3361 caaaaaggtg gggctccgat tacgatcgac ggaactttag atgaaatttc agaaaattct
     3421 actgccactg agaaaggtgt tttttatgca gtgaaaggaa cgttaaaaac aactcaatcg
     3481 tttccgtttc gttatggatt aactggagag ttgtctttaa ttgtaggaga aaaaacatat
     3541 tggcaacaaa ttgtcgatgt gtttctaaat aaaaaataat tatttttact aagaatatag
     3601 gaggagttta tatgaataaa agaagatgga ttgctgtggg agtagcgtgc ggattattgt
     3661 tactatccat tatcgtcgcg ctaattccag gaaaagataa agaggaagca tcaaatacaa
     3721 ccctaacagg aataaataag attttttacg gatctaacga aatcacagaa gaaacgttag
     3781 aagaaggcgc atcaaataaa aagattgtca aactatccgt caatggagtt attgcagata
     3841 caggtgaaag caatcttttt agtagggaac aatataacca tcagaatttt ttaacacagc
     3901 taaagaaaat tcaagaagac aaagcggtta agggagtgtt attagaagta aattcacctg
     3961 ggggaggaat atatgaaagt gctgagattg ctaaagaaat ggcaaaaatt aaaaagctag
     4021 atattcctat ctatactgca tttaaaaata cagcggcaag tggagggtac tacatttctg
     4081 ctggttcaga taaaatattt gctacggaag aaacaacaac tggatcaatt ggtgtcatta
     4141 tttctggttt aaattattct ggcttattag aaaaacttgg cgtaactgat gcgacgtata
     4201 aaagtggtgc attaaaagat atgatgtcac ctcaacacaa accttcagag gaagagaata
     4261 aagttataca ggaatttgtg atgagtgctt atgatcgttt tgtcaatgtt gtagcaaaag
     4321 gaagaaatat ggatactaat gcagtgaaag aacttgcgga tggaagaatt tatgatggta
     4381 accaagcagt agaaaatggg ttggtggatc aaattggtta ctctgaagat gcattagatt
     4441 ccctaaaaaa agagaagaaa ttaactgatg cgacggttat agaatataaa aatgacacaa
     4501 ctggctttgc ttcttcttgg ttaggtaata agattgctga atggcaaggg ctaaaggcaa
     4561 ctacatcaga tcgtgtaatt tcagtttttg aaaaacttgg attttctgat actccaaaac
     4621 caatgtatta ctatggaggg ctataaaatg aataagaaga gtggcattga aactttgcct
     4681 aattatgtat ttgcgggatt ttggttacga ttttttgctt ttcttattga tttaatttgt
     4741 attagcatca tcacccatgc tactataggt ctcttttaca aaatcatggg actggaatct
     4801 gacaaatcca ttattacttt ttatggtata gcgtcattag ctatttattt aacctatttt
     4861 gtacttttga ctaagctgaa ttcaggacaa acaattggga aaatgatttt tgggataaga
     4921 gtagtgagct ttgaggaaaa agagctaagt tggcaaacta tctttattcg tgagtgtata
     4981 tgtaggttta tattaaaaaa ttttgtattt caaattgggt atttggtggc tgctttcacg
     5041 cccaagaaac aacatattgg ggattatttt tctaacacgt cagtggttgt tattcgttca
     5101 ttagaagcta ttaactatgg tcaaaaaatt tctaattcta aaacacttaa tctggagaga
     5161 attaaatgaa taagagaaaa acagagttta ttttgatttt aattagtgga ataacaggga
     5221 ttttatgtag tcttttagca ttgatgggtg gtatgtttct atcttctatt gattcaacta
     5281 ctttaaatac tacagaacta accagtaagg atatagatat gttagtcaat ctaagcggga
     5341 atgttgtttt atttggagga attaccctag ttttttcgat aatgattttc ataataggtt
     5401 ttttagtaag aaaaaatcaa aaaacagtgg tctatggtat tagtttactc attttatcta
     5461 ttattccatt ttttctttta acattattgt gggtaattcc tggagtttta ggtattattg
     5521 cgggattgat gcttctaata agaaaatctt aattaggcaa tgtgtagaca acgtgaaacg
     5581 attaatcaat taaaagctaa atacgttttg agaaatgata acttgtagtc gatggaagac
     5641 atgtagataa aaaaactacg tgtcttttat ttattctaat tttttcgaaa aaaagacact
     5701 ctctgattgt tgtattaatg gaaaagggga aagcagcaag taaatttagt ttttctatta
     5761 aatattcttc actttttaac cttttatagt acttagattt aatcctatat gtttagagag
     5821 ttcattggca gcatgatttt gcaaagcttc tcgaattatt gttatattag aaactttact
     5881 gttcataatt atgcttcttt tttaactcaa agtccaaagg gctacagtgg actttgagtt
     5941 aaaaataaaa agccctagtt attttgaaaa ataaaaaaag aacagcgtaa atgttttcga
     6001 aatgctttcg caccaacatt tacctgttcc gtgggtttgg cacccaatgg gccgtgaggg
     6061 gctcgaaccc gcgacccgct gattaagagt cagctgctct accaactgag ctaacggccc
     6121 gtacaaaaag tacttaataa tagtagcact aagaatttta aaatgcaaga ctacatttaa
     6181 agaaaatcag aaaaatcttt acttccttat aggaaatcat tcatagatta ttcacatttg
     6241 ctcgctatta tataagcata ccaacaaaca aagttcttgc aagagaacaa accctaacac
     6301 ttttttcatt ttttactcct ttcccgccgt ctccccggcg ggtctcctgt tcttaacgat
     6361 aaaagacaag aaggcttagc cttcttgttt tttgttttca tggaatcccg ctaatcaaat
     6421 cacctagtta aagcaactag attgaatgtt tatcataagt ctgatatcat gtttatgaaa
     6481 tgaaaaaagg agcggttatg atggaaaaaa tgcgtcagca attaatggag tgggggaagg
     6541 aactagaaac attccgtctg cctcactggg aagaactccc tgacttagaa ctttatatgg
     6601 accaagtcag aacgttagtt gatcgttatc tttcacctgt gatacaagga gaaaagcatc
     6661 cattgcttac ctcttctatg gtaaacaatt acgttaaact aggtttgata ccagcaccag
     6721 ttaaaaaaag atataacaaa gaacacgtag catttttact tgcaatcact acgttgaaac
     6781 aagtattgac tattcctgaa atcaaagaag ggatattgtt ccaaggaaaa acagtaggaa
     6841 ttcgtgaagc ctataatcta ttttgcgatg aacaagaagc agctgtgtgg atggtcagcc
     6901 aattagcaca aggtaaatct catccgcaaa aattcgacca ggcaacccct gtagaatata
     6961 ttgctgtaag agcggcaact ttatcgtttg caatgaaact tcttgcggaa aaaacgattg
     7021 ttctagaaac cgaatattta aaagaggaga aaacaaatga aaaacaataa aatcgcaatt
     7081 ttagtagatt caggtacgga tgttccagaa gaattgataa aaaaatacga gatgtatatg
     7141 atcccattga aaattattta taaagatcag gtatatacag ataaaattga cattacccct
     7201 gaagagatct atcaacgttt gccagaggaa atccctggta catctttgcc ggatggtgag
     7261 tcgatcacag aaatctttga gcaaatcaaa aaagacggtt atgaaaaggt acttgcggta
     7321 acaatttcca gtggattgag cgggacatat aatgtggttc gtttaattgc acaacagcaa
     7381 gaagaattgg aagcatatgt actagatacg aaaaatattg gtattggtgc aggtttttct
     7441 gcgatccaag cagcaaaatg gctggaagaa ggagtagaat gggatcaact gatcagtaat
     7501 ctgaatgagt tagtcgaaag aacaaaagtt ttctttaatg ttgcgacatt ggaatatctt
     7561 caaaaaggtg gacggatcgg tttagttgct tctattgtag gaaccgcttt aaagcttaac
     7621 ccaatcatct catgtaatga agaagggatt tattacacag taggaaaagc taggggacgt
     7681 aaaaaaagtt tggataaaac ggttgcttac gtcaaagaaa aaatcggtaa tgcaaaggtc
     7741 ttcaacttag ctgttgccca tggggatgcg aaagaagaag caattgaaat ggaagcgaga
     7801 cttaaaagag agttcccaca agcaaaccaa atctactttg gccagatttc tccggcatta
     7861 gtagtccaca ctggtccggg attattgggt gtcggtgtac aagttttaga aggataaatc
     7921 aaaaaaagga aacactgagg tgaaaatcag tgttttcctt ttttattgaa gttagaacaa
     7981 aaaataataa agtattttgc actttcattt taattttctc ttttgtttta taggcaaaga
     8041 tgaaacaaaa gcagaaatac ggtataatga gaaggtatga caataatata taagaggtgg
     8101 ctctttatga aaaaagtttt agttgtcgat gatgagaagc cgatttcgga tatcgtcaaa
     8161 ttcaatttag ccaaagaagg ctatgacgtt tatactgcat atgatgggga agaagccttg
     8221 gaaaaagttg ctgaggtaga acctgatttg attttacttg atttgatgct acctaaaatg
     8281 gacggcttag aagttgctcg tgaggtacgc aaaacttatg atatgccgat cattatggta
     8341 acagccaaag attctgaaat tgataaagtc ctaggcttag agttaggcgc cgatgattac
     8401 gtgaccaagc cattttccaa ccgagaatta gttgcacgtg tcaaagcaaa tcttcgtcgc
     8461 ggtgccacgg ctgcgaagga accagaagag gcagcacctg cggaattgac gattggtgat
     8521 ttgacgatcc atccagaagc ttacatggtg acgaaacgag gagaaacaat cgagttaaca
     8581 catcgtgaat tcgaactact cttttacttg gcgaagcacc ttggacaagt gatgacaaga
     8641 gaacatttat tacaaacggt ttggggttat gattattttg gagatgtacg aacagtggat
     8701 gtaactgtac gtcgtttgcg tgaaaagatc gaagataatc caagccatcc aaattattta
     8761 gtgacacgtc gtggtgtcgg ttactatttg cgtaacccag aacaggagta attggatgaa
     8821 aagaaaagtc cgcttttttc gatcagtcaa ttttaagatc gctattacct ttattctgat
     8881 tttattgatc tcgatcgaaa ttattggtgc ctactttata cgtggtttag aacgttccac
     8941 gatcaataca tttattaaag atatgaatca aacggtggaa tcattagcaa caacaatcag
     9001 cccggaattg aatcgaaaag ataatgcaga tgatgaagag gtaaatgcga atatcaaaag
     9061 atttattgaa aacagtgcga ctagcgatat catcgaaata cgggtcgttg acgaaaaagg
     9121 gatcatccga ggaacaaccg atgtcaatga acaaagcgct gtgggaaaga aaaatgatta
     9181 tgttgatatc aatgatttca cgactaaacg ttatgttgct ttagataacg acgataaacg
     9241 ggtgaatatc aatgttcaac cgatcctgtc cccgaccggc gatgccgtta tcggggctct
     9301 ttacgtcaaa agtaatatcg aacaaaaata ttcggaaatc aataacacag cagtcatttt
     9361 ctttactgcc tcactaattg cggcatttat ttcaatgatc gtatctgttt tagttgctcg
     9421 ttcgattaca cagccgatcg gtgaaatgag agaacaagcc attcgaatcg ctagaggaga
     9481 ctacagtcgg aaagtcaaag tgcatggaca agatgaactt ggacagctgg ctgatacctt
     9541 caatcagtta gctgaacgta ttgaagaagc tcaagataca atggaagctg aacgcaatcg
     9601 tttggatagc gtgctatccc atatgacaga cggcgtcatt gcgacagatc gcaggggtaa
     9661 agtcattacg atcaatgata tggctgtttc cttgctagat atcaaaaacg aggaagcaat
     9721 cggacaatcg attttgacac ttttagatat cgaagaagaa tatacgttgc ggaaattatt
     9781 agaagatcca aacgaaatgg tacttgaccg ttcaaccagt acattggaaa gtgatcagat
     9841 gatcttgcga gtagactttt ctatgatccg tcgcgaatca ggttttatca gcggattagt
     9901 agctgtcttg catgatgtga cagaacaaga aaaaacagaa cgggaacgtc gtgaattcgt
     9961 ctctaatgtt tcccatgaac ttcgtacacc gttgacaagt atgcgtagtt atatcgaagc
    10021 attgagcgaa ggtgcgtgga aagacgagga agtagcacca aattttttga aggtaacgct
    10081 agatgaaaca gaccgaatga ttcgaatgat caatgacctc ttaaatctat ctcgcatgga
    10141 tacaggtaat acccagcttc aattagaata tgtcaatttc aacgagttag tgaattttgt
    10201 tcttgaccgg tttgatatga tggtgggaaa tcaggaaaaa aattataaga tccgtcgtga
    10261 atttacgcaa agagatcttt gggtcgaagt agatacagat aaaatcatcc aagtagtcga
    10321 taatatcatg aataacgcga tcaagtattc accagatggg ggaacgatta cttgtcgctt
    10381 attagaaacg cataataacg tgattttaag tatcaccgat caaggattgg ggattcctaa
    10441 aaaagatctg aaccgagtgt tcgagcggtt ctatcgagta gataaagcac gtgcaagagc
    10501 acaaggtggg acaggacttg gattagctat ttctagagaa gtgattaagg cgcatcgagg
    10561 cgctatttgg gcagagagca aggaaggaaa agggtcgact ttttatatct cgctgccata
    10621 tgaaccttat gaggaggaat ggtgggaatg agaatctctg aaaaagtcgt tcgtatagga
    10681 ctcattttga tggttgcgtt aagcatatac ttctcttatg caatctggct gagcccagca
    10741 ggaaaaagtt caatcaacgt agatgagaca aacactcaaa tgatcgagtc tcaaagcaat
    10801 cgaaaaactt ccgaagtctt tcttccgctt catgttacat ggcaccattc gggaatgatc
    10861 caagaaacca acagtgaaaa tctcgtgtac cgattgcaat tgatcatcga taaagcaaga
    10921 tttggaaagc tttctgaagt agtttcagga gacaaatcac agtttgaaca ggtgaaaaat
    10981 ctgaactctg gttttgagtt ggcctataat gcgccttttt tgctaagaga atacaaggaa
    11041 gcatttgatt tagatttaga tgtgtcgaac ttggcaaaag aaagcgaaaa tatgtatttt
    11101 actagaatac aattcgatca aggaaataaa aaaatccgat ttttgaatta ttcgaaccat
    11161 ctcgtatatg aagcaaatat ttcgttgaat tggacagata tcaataaaga attaacagca
    11221 aaagacacaa aatggacaga aatgacaaca gaaccatcga cgatcgacac gcaatatgct
    11281 acgaaacatt cgatcaaact gaagaagtat agttatatcc tgtcacagca accctataca
    11341 ctttttagaa atgctttttt ccagaaacca gatagcgtga aaaataatga tgaaagcaat
    11401 gaattgattt tttacggtgg gaatgaaaca atgtctatcc attcggaaac gcaagtagtt
    11461 gattttcgtg gagagctgcc agagaaaaaa gaaaatgcta gtgtattttc tgaaagtttc
    11521 ccctatatta gcaaattagg cagttcttta gggaacctca gatattttga ccgcagtgac
    11581 aatacgatcg attatcggat attcgtggaa ggcttcccgg tttttgggac agacagcaga
    11641 ggacagattg gtgtggaagt tggcggtgaa acgaatggtt ctgtgcaagt gaatatccaa
    11701 accagtttga atacgattca agtgccgata ccttcagacg aagaagtaga acttgcaagt
    11761 acgaatgaaa tcatccagca gttaaaagct aagggagcag atagcaacaa gattggttca
    11821 gtgatcgttg gctatacatg gcaaaatgtc aaagaaacga atcgtgtcgt tgatctctta
    11881 cctgaatggt atgtgaaata taaagataac tggtattcag ctaacgacct tctggaaaaa
    11941 ttacctggat tggaggcgaa ttaattggat ttcaaaaaaa tcgaatggat atttttcgtc
    12001 gcttttttag ggttgaatgt gtttttattc agcagttatc atgacgcggt ataccaagaa
    12061 aacaatgtca ttcgctctaa tcagactgaa tcaatccaac aacgtctggc tagcgacgat
    12121 atcacttaca cggggaagat ttcgtctgag aacaagcaag gctattatct tagcgcggag
    12181 cagaccaatc ttagcacttc attagcgaat tatcgcaagg ctggtaaaag cggcttgctg
    12241 gcaagtggtg tgtttttaga cggtgatgtt ttgacgaaat ctttatccag ttcagatgct
    12301 gaaaagaacg tgatcgatcc agataaaatt agtagttcct tggaagattt tttatctaac
    12361 aaagacaata ttttattcgg taatgattat gtgtacacga aaaacttttc tcatttagaa
    12421 gaagaaccac tagaaatcca agcaagccaa gagtatgaag gcatcccatt caatgatgat
    12481 acagcaaaaa tcgttctttc tttagaaaaa aacgataatg attatagcat cacgaaatac
    12541 atgcagacgc atttatctga tatcgagcag ctgcgcgaaa agacggaact gtatacggaa
    12601 gaggaagcaa tcaatacatt gtacatcaat aacaaaatac caagaggatc aaaaatttta
    12661 tggcgtcagc tagcttattc ctgtattctg aaagtccggg aaaaaaatgt gtatgttcct
    12721 gtttggcatg tagcgatcga aacaagcgat aaagtggtcc aaattgagag cgtcaacgct
    12781 ttcagcaata cgatcgtgac aaacaatact gtaccaaaag tggaagatca ataaaaaagc
    12841 gagtataatg aaaaaagttt acaaagtaag aagggaagta ggagtcaaat ggctgaagaa
    12901 aaaccagctt ttaaaatcag tatcctagca agtggaagtt cgggtaattc actttatatc
    12961 gaaagtgaaa aaaaacggct tttggtcgat gcaggactta gcggtaaaaa aatcacttcc
    13021 ttgatggctc aaattgatcg gacaccggac caattagatg gaatacttgt gacgcatgaa
    13081 catcgagatc atatacatgg cgtgggtgtc ttggctcgta agtatcattt ggatgttttt
    13141 gccaatcagc agacttggga agcgatggaa ccactgattg gaaatgtacc agtggaacaa
    13201 aaacatctat ttgagatggg gaaagtcaga acattcggcg atatcgatat tgagagtttc
    13261 ggcgtctccc atgacgcagc ggctccgcaa ttttatcgtt tccataaaga aaatcggtca
    13321 ttcgtcatgt tgacggacac cggatattgc agtgattata tgcgaggtgt cattgaaaat
    13381 gccgatggtt atttgatgga aagtaatcat gatcttgaga tgttacgcat gggaccttat
    13441 ccgtggaatt taaaacagcg tattttagga gatagaggac atctttccaa cgaagacggt
    13501 gcacttgtca tgactgatgt gatcggtaat cggaccaaac gaatctatct tggccatctt
    13561 agcaaggaaa acaatatgaa agaacttgct catttaacaa tggaaaatgt tttgaaagaa
    13621 aaagattttg gcgttggaca tgattttgaa atctatgata cagatccaga tgaagcaact
    13681 gagctatttt cgatttaaac agttcaatca ataaaaatat aaaaagcaga agataaaatc
    13741 aatcagggtt ggttttatct tctattttta tttttttagc atattaaaaa aataaccgta
    13801 ttatcgactg actacactga tgtgctgctt ttcatgtttc tcatccagta tctcatctac
    13861 taatgcaatc gcgtaatctg ccatgctgat cgtactgtcg ctagcagcgt ttctttgtaa
    13921 ataatcatca cttaacaaat aagtacctgt acgtttgcca tgtggatcaa ataaagaagc
    13981 tggactgata taagtccaat gtagattttt agattgtttc aattttaaca atccttcgta
    14041 catattgaaa gctgttggat agaagggtgc atttttaggt acttggtcaa tcatacgagt
    14101 cgtcttttct gtatctaaga ataacgaaga ggcaccccca acgacaatta agcgtgtatc
    14161 ggttccttca agcatctcaa tcaaatggga caaagaagtt tgatgcattt cttcttttcc
    14221 tttgggagcg ttgaaagcat caacgacaac atcaaaagat gcaagatcag tagttgtcaa
    14281 ctcaaataaa tccttttgta taaacggaac atctgatggc aatttttctg gatgacgcac
    14341 gattgccgtc acgtctaaat ctctggacaa tgcttcgtct aaaataaatg aacctgcatg
    14401 gccagtagct ccaatgagtg caattttcat aaaaaacctc cttttttatt cctgtccatt
    14461 gtaagcagtt gctgaatatt cgtcaaagaa tccgattttt gaaaagctca gtgcgcattc
    14521 ctttcatggt tcgtgatcat tgggtatagt taaattaaga aaactgattg ataaaaatag
    14581 aagttgaagg agataagaaa tgaaagagtg gttagatcgt gcctttttac caatgataac
    14641 atctgttgtg aatcttagac ccttaactgc tttgcgtaat ggaatgactt acacgatccc
    14701 aataattatt gttggctcgc tgtttttctt atttgctcat tttcctgtgg attacatcgc
    14761 tcagtggata gacaaaacag gattttctat gtattttaca caagtatacc aggctacatt
    14821 tgatgtgata gccttttgga tcgtttttgc cattgcatac gcttatgcaa aagaagaaga
    14881 catcgatcgc ttaccagtcg gcataacagc cttgtctgct tatctactgc ttattcagcc
    14941 actaaatact cagatagata ctgtctggac aggtgctgaa gggatactag gagcagttac
    15001 aactgggctc atcatcggat ggggctatgc acgtttagta agacttgcga aagggtggaa
    15061 gcaaagagag aagatacctg aaaatgtttg gcagtctttt acttcattga ttccaatcat
    15121 tgttatagtc acttttacgc ttatcggttc agcggttttt caacatttgt tcagaatgac
    15181 tgttgttcaa ggagtatgga catatgtcca gcttcccatt caatcgttca tagacacgtt
    15241 gtttggtgtg cttttattag gtttttttgt tccgttttta tggttatttg gtcttcatgg
    15301 atctgcgatg gtcaatgggt tggttagtcc cgtcttgcaa gcgaattcat tagcaaatgc
    15361 agaaatctta gcctcaggac aagagttgac ggtggcaaat ggcggacata tcgttactca
    15421 gcaatttctc gatcagttca tgacggttac gggtgctgga ttgacgttag gggctgtatt
    15481 ttttatgacg gtttttgcga aatcgaagaa atacagagaa ttaggaaatc ttgctctttt
    15541 accagccttt ttcaacatca atgaatcaat tattttttcc acaccgatcg tgatgaaccc
    15601 gatgatggct gttcctttta ttttcgcacc ggtcgtttca ggattgatta cctattccgc
    15661 tttatatttt ggattcgttc ctttatttac agcagtccaa gtcccttgga caacaccgcc
    15721 gatcctctcg ggtttcttag taggcggagt gcctgcagcg attttacaag gtatcgtgtt
    15781 gtcaattagt tttttcattt atttcccatt tttgaaaaaa atagattctg caaactgcaa
    15841 aaaggaaaga cagacaaaaa atgatggagc agcagaaaag atgaacgact aaaaaacaaa
    15901 aatactcctg tcgctttaga ttgggaaaat caccaatcta ggatgacagg agtatttata
    15961 gtcatgttta tactagccct ttttggacag ctaattgaac aagaacaact aatgtgttca
    16021 ttcctacgtg tgtgatcatc gatgtccaga tccgaccagt tgctttgtac tctaggctga
    16081 agaagaaacc taggaaaaag tagattagca gatgaccatc attatgagca aacgcaaaca
    16141 gcaaagagct gatcactgct ccgacccaaa aatttgaata tcgactgatg atccccagta
    16201 tactgcggcg gaatacgaac tcttccatga tgggtccagc aacagttgtc gccaaaataa
    16261 aagcgggtgc ttccatgatc atctggatga tattttgtgt attctcggaa ggggttgctt
    16321 tgccaaacaa tacagattct aacatgacag cgatgctttg cagaatcaat gcgacaaaga
    16381 ttccaaccaa tccataaata acaatctttg gagcagaagc tttttttacc gctgattcga
    16441 tcgctaaggg ttcacgttgc ttaaaataaa aaatagctag gacaacggcg cctagtatat
    16501 acatgactgc agttgctgtg atcaattcag acgtagattt tacaagtcct atcgaagcta
    16561 ggatcaaggg tgagataaaa attagaccat agcaaaaaat gctgaggaag ctatattttt
    16621 ttaaagacat gtaattcctc cttttaaggg ataacaagca tgcaaggcaa gcttatatcg
    16681 attgatttca attatatcat aatttcaata agcagctgtc agaataaaat ttcttttctt
    16741 tgatttaaaa gcttgcaaaa aagagggaga atgagtatta ttaaaaatgt aggttagcac
    16801 tcgaaggtta agagtgctaa cagattaaaa aaagagttat cggagggatt tatcgtgtta
    16861 aaaccattag gtgatcgcgt cattatcgaa gtcgcaaaag aagaagaaaa aactgtcgga
    16921 ggaatcgttt tagcttctgc tgctaaagag aaaccccaaa ctgggacagt tgttgcagtc
    16981 ggtgaaggcc gtttgttaga aaacggtgaa aaagttcctg ccgctgtaaa agcaggcgac
    17041 caagttatgt tcgaaaaata cgccggtaca gaagtaaaat acgaaggaaa agaatatctg
    17101 atcgtcgcag gaaaagatat catggcgatt gttgagtaaa tttggaagat aaaaaaataa
    17161 tcaaaaataa tcaatgaggt gaatcaatca tggcaaaaga attgaaattt gcagaagacg
    17221 cacgcgcagc tatgttacgc ggggtagata aattagcaga tacagtgaaa gtaacattag
    17281 gacctaaagg ccgaaatgtc gtactagaaa aatcttacgg ctctccattg atcacaaatg
    17341 atggtgtgac gatcgcaaaa gaaatcgaat tagaagatca ttttgaaaac atgggtgcaa
    17401 aacttgtatc agaagtagca tcaaaaacaa atgatattgc aggagacggt acaacgacag
    17461 caactgttct gacacaagca atcgttcgcg aaggtttaaa aaacgtaact gcaggtgcta
    17521 atcctctagg cattcgtcgc gggatcgaat tggcaacaaa agcagcagtc gaagaattgc
    17581 ataatatctc aactgttgtt gattcaaaag aagccattgc acaagtagca gctgtttctt
    17641 cgggttctga taaagttgga catttgattg ctgatgcaat ggaaaaagtt ggaaacgacg
    17701 gtgtcatcac aatcgaagaa tcaaaaggga tcgaaacaga actagatgtt gtagaaggta
    17761 tgcagtttga tcgtggttac ttatcacaat atatggtaac agataacgac aaaatggaag
    17821 ctgttctaga aaacccatat atcttgatca cagacaaaaa aatctctaac atccaagata
    17881 ttctgccatt gttagaacaa atcttgcaac aatcacgtcc attgttgatt attgctgacg
    17941 atgtagatgg tgaagcattg ccaactcttg tattgaacaa aatccgtgga acattcaacg
    18001 tagtagctgt aaaagctcct ggtttcggcg atcgtcgtaa agcaatgctt gaagatatcg
    18061 ctattttgac aggtggtaca gtaattactg acgatctagg tttagaattg aaagatgcaa
    18121 caatcgaaaa cttaggaaat gcttctaaag ttgttgtaga taaagacaat acaacgatcg
    18181 tagaaggttc tggagaaaaa gaagcaatcg aagctcgtgt gcaattgatc aaaaaccaaa
    18241 ttgctgaaac aacttctgat tttgaccgcg aaaaattaca agaacgctta gctaaattag
    18301 caggtggtgt agctgttgtt aaagttggtg cagctactga aacagaattg aaagaattaa
    18361 aattgcgcat tgaagatgca ttgaatgcta cacgtgccgc agtagaagaa ggaatggtct
    18421 caggtggtgg tacagctcta gtgaacgtta tcagcaaagt ttctgctgta gaagcagaag
    18481 gagacgtggc aacaggtatc aagatcgttg ttcgtgcttt agaagaacca attcgtcaaa
    18541 tcgctgaaaa cgctggttat gaaggatcag tgattgttga taaattgaag aacgtggaac
    18601 taggcactgg tttcaatgct gctactggag aatgggtaaa catggttgaa gcaggtatcg
    18661 tagacccaac taaagtaaca cgttctgcct tgcaaaatgc cgcttccgtt tctgccttgt
    18721 tattaacgac agaagcagtc gttgcagata aaccagaacc agcagcgcca gcagctcctg
    18781 caatggatcc atcaatgatg ggcggtatga tgtaagaaaa agcaacttgc ttatctacaa
    18841 ctgatcatta gtgaaagaca gataaaaaga ggctggaaca gaagcgttta actccaagaa
    18901 ataagaagaa attcacggaa attgcttttc aaatttttgt gaatttcagc ttatttccga
    18961 aggagttgct tctgcttccg ctgtttatac gtgt
//
LOCUS       DAFGZE010000054        18394 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.056, whole genome shotgun sequence.
ACCESSION   DAFGZE010000054 DAFGZE010000000
VERSION     DAFGZE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.056"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(186..752)
                     /locus_tag="LTU54_002015"
     CDS             complement(186..752)
                     /locus_tag="LTU54_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL8296721.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(752..1708)
                     /locus_tag="LTU54_002016"
     CDS             complement(752..1708)
                     /locus_tag="LTU54_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBL8296722.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1708..2364)
                     /locus_tag="LTU54_002017"
     CDS             complement(1708..2364)
                     /locus_tag="LTU54_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBL8296723.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2734..4752
                     /locus_tag="LTU54_002018"
     CDS             2734..4752
                     /locus_tag="LTU54_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL8296724.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4822..5028
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5179..7593
                     /locus_tag="LTU54_002019"
     CDS             5179..7593
                     /locus_tag="LTU54_002019"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBL8296725.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7941..9494
                     /locus_tag="LTU54_002020"
     CDS             7941..9494
                     /locus_tag="LTU54_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBL8296726.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9875..11659)
                     /locus_tag="LTU54_002021"
     CDS             complement(9875..11659)
                     /locus_tag="LTU54_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296727.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11674..13395)
                     /locus_tag="LTU54_002022"
     CDS             complement(11674..13395)
                     /locus_tag="LTU54_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296728.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13495..14256
                     /locus_tag="LTU54_002023"
     CDS             13495..14256
                     /locus_tag="LTU54_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL8296729.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15223..15491
                     /locus_tag="LTU54_002024"
                     /pseudo
     CDS             15223..15491
                     /locus_tag="LTU54_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16163..>18394
                     /locus_tag="LTU54_002025"
     CDS             16163..>18394
                     /locus_tag="LTU54_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296730.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
BASE COUNT         6036 a         3284 c         3719 g         5355 t
ORIGIN      
        1 tcctgcatca agtaataaga acagccaatc aaaaacagct tctcatgttt ttgatttggt
       61 tggacttatt accgcagata taagcttttg agcaccgtga ataaagaggt tatgaaatca
      121 acgctctaac ctgagtatta gagaaaaaaa cgcaaaacat atcatttatg ttttgcgttt
      181 tttatttatc tttttttctc aatacaatat aaaataggcg gctggttctt ttggttgata
      241 aaccgataat tcagcacatt atatgtttcc tgtggcagtt tggaacaaaa agaatcaacc
      301 atatccagtt ctttttctcc accttcatga ccatagtaga cgacaaggat caatcgtccg
      361 cgaggcacaa gtcgtttcaa tatttcttcc atagctgtgc gtgtagtctc tggcatagtg
      421 atgattgctt tatcactttt tgggagatac cctagattga aaatccctgc tttgagattt
      481 tgttgttcag ctatcacttc tcctaacgtt tcgtggcctt gtagaaacaa tgagacacgt
      541 tcttctaact gacgctgttc tagcctttca cgagtagcat cgattgcttg ttgctggata
      601 tcaaaactat agacatgacc agtttttccg atgtgttctg ctagaaaaac cgtatcgtgg
      661 ccattgccca tcgttgcatc tacgacgtga tcccctggtt gcaagatttc ttgcagtaat
      721 gtatggctga aatgcaaagc agtttgcaac atcagacgct cacctttttc cgtacatcat
      781 attttccttg gaagctatct ctgcgaagaa gttcttcatc aattgcattc aagacttccc
      841 attttttcaa gctccacatt ggtccaatca aggaatccca cggcgcatcc cctgtcaaac
      901 gatggatgat gatctctttt ggtatcattt ccagctggtc gcaaatgaca ttgacatagt
      961 caggacggct cattaattga aggcgacctt catgatagtc ccgcaacatt ctcgttttgc
     1021 gcatcagatg catcaaatgg agcttgattc cttggatatc tgaatctaaa atggtgcgtc
     1081 tgacattttc tttcatcatt tctaaacttt cgcctggtaa tccgttgatc aaatgcgtac
     1141 aaacacggat attatgtttg cgtaatttag ccacgccatc taaataagtt tgataatcat
     1201 gtgctcggtt gatcagtttt gaagtttctt caaaagttgt ttgcaatcca agttctaccc
     1261 ataaatacat ccgctcgttc agttcagcaa ggtaatcaac tacttcatct ggcagacaat
     1321 ccggtcgtgt accgactgat aatcctacga cacctggtaa attgactact tgctcaaatc
     1381 gttctttgat tatttcaact ggcgcatggg tgtttgtaaa attttggaaa taaacgatgt
     1441 attgttggac atgaggccat ttttgatgca tcatgtcgat ttctttctgg aattggattg
     1501 gaagagggtc ttcaggtgca acgatcatgt ctcccgaacc agatacactg caaaaagtac
     1561 aaccgccatg ggcaacagtc ccatctcgat tcggacagtc aaatcctcca tcgatcggca
     1621 ccttgaaaat tttcccgcca aactcatttc gtaaagcgta attccaagaa tgatatcttt
     1681 tatttccttc tgaataagga aaagtcatta aaagtccttc tttctgtaag agttatctat
     1741 ttgttttctt ttttctctat acactggata agtcaggcac agccaagaaa gactgagtaa
     1801 gtagccgccg atgatatcag taggaaaatg aacgcccaga taaatgcggc ttatcccgac
     1861 acttactata ataaacatca tcagcaattg aagcaatgtg cgtaaggttc ttttttgaac
     1921 aaaagctgga agcagaaata gtaaagaacc gtaaagaatc atactgcctg ctgcatgtcc
     1981 actagggaaa ctgtaacttg tttcaactac taagtgcgcc aatgaaggac gctctcgcat
     2041 gaaaaatagt ttgatcaccg ggttgatgat tccagcaatt acaataaatc cagaaacgaa
     2101 ccaaaatgtt tctgcccttt ttttcttata ccacatccat aaagccgcac aagcaaataa
     2161 aatgatgacg gtaagaggat taccgaattt agtcataaag agttggtagc tgttcagatc
     2221 aggataagaa gctctgacaa tgcctgtaaa ccactgatcg aaaccagaaa gccacgacag
     2281 atggaacttt acgatccagc ccaaaatgaa aaacaggaca agaaagaagc taccagccac
     2341 ctgaaatgat gttttatatt tcatacaatt cctcctttga agcaatgact tattatagca
     2401 tttttggtcc agcgaaaaaa acggtaaaga gataaaacca atgtatgatt cctctttgaa
     2461 taaccaatat tcttataaaa aaaataactg taatcggaaa gacatttttt ggtaatgcaa
     2521 atgaaatgtt agggaaataa aatcagtgtt tttcgaaatc cttttaaaca agaacgtaga
     2581 cgtaggaaga acttgaaaat gaacagaagt tgttcattac ggtaaattac aatatgatta
     2641 caaaaaacaa caaagctctt aaataacttt taataaatat taagagaagt tataattatt
     2701 aataatagat aaattgaagt tggaggatag aagatggaaa acattgcaag aaaagaacgg
     2761 agacgtttag aacagaccaa acgttttcga aaagcaaaac gaggggcagc aatcgtggga
     2821 actgcgatgg taggctgttc tgtcgctgcc ccattagtac agccggttcc tgttcaagcg
     2881 gatgaaacac ctgttcagtt cggcgcaaga atcaatacag cggcatttat cacagaaatt
     2941 gctacttatg ctcaaccaat cgctcaagcc aacgatctat atgcatcagt gatgatcgca
     3001 caagcagttg tagaaagcgg ttggggcagt agtgcgttat ctcaagcacc ttattataat
     3061 ctttttggga taaaaggcag ctatcaagga caaaccgtct acatggatac gcttgaattt
     3121 ttaaataatc aatgggtaaa taaaaaggag cctttccgac aatatccttc ttttgctgaa
     3181 tctttcaatg acaatgccta tgtacttaga aatacttcat ttggcaacgg ttattactat
     3241 gccggtactt ggaagagcaa cacgaaatcc tatacagatg cgacagcttg tttgacaggg
     3301 cgttatgcga ctgatcctgg ttacgcaggc aaattaaata atatcatcgc aacttacgga
     3361 ttgacccaat atgatacacc agctagcgga aacgcaggcg gtggagcgac tacaggtggc
     3421 aataatactg gaagtgcagg tagtactggc ggttcaagca attctggtaa tacaaactca
     3481 ggttcagcaa cttcgaccac ttatactgtc caatctggtg attctgtttg gggaatctca
     3541 aataaattcg gtatcacaat ggatcaattg atccaatgga acaacatcca aaacaatttc
     3601 atttatccag gacaaaaact gacaatcaaa ggtagccagg aaaatggctc ttcgacgaat
     3661 aattccggta acaataccaa ctcttcagga aatgctggta catcaaacgg aggacaaaca
     3721 actggtgcga aatacactgt tcaatctggt gattctgttt ggaagatctc aaatgatcac
     3781 ggaattacga tggctcaatt gattgaatgg aacaatatca agaacaattt cgtttatcca
     3841 ggacaacagc taatcgtatc aaatggcggt tcttctaatg ctggagcaaa tactggctca
     3901 aatactggga atacaagtgg ctctagttcg tccagctcaa gttcttctaa tacagcttca
     3961 ggaacgaaat atacagttaa agccggagac tctgtttgga gcgtctcaaa taaatacgga
     4021 attacgatgg ctcagctaat ccaatggaac aatatccaaa ataactttat ctacccagga
     4081 caacaactga ctgtttcaaa cagtggatca acaaacagca cgtcaggtaa tacaggttcg
     4141 tcaaccaata caaataacag cagttcagtt aatactggca gcagttcgtc tcaaggcact
     4201 tataccgtga aagcaggtga atctgtttgg agcgtggcaa ataaaaacgg catcacgatg
     4261 gatcagttaa tcgaatggaa taatatcaaa aacaacttca tttatccagg acagcaattg
     4321 atcgttaaag gtggaacttc ggcaaataca aatacaggtt caactacttc tgcagcgaaa
     4381 ccaaatacac cgaatacgtc tgctacgaca agtacatcat ccactggtaa tacgatgtat
     4441 accgtgaaag cgggcgagtc agtttggagc gtagcgaaca aacatcatat cacgatggat
     4501 cagttaatcg aatggaataa tatcaaaaat aatttcatct atccaggaca gcaagtgatt
     4561 gtgaagaaag gttcatctca atcaacaaac caacaatcag ttactggttc gaaaacctat
     4621 actgtgaaat ctggagaatc tgtttggagt gttgctgaca gtcatggaat tacaatggct
     4681 caactaatcg aatggaacaa tattaagaac aactttattt atccaggtca aacgttgatt
     4741 gtagctaaat aaaatagctg ttcgaagaaa taacgattga attccacaaa aatatttgtt
     4801 actataaggc tacttaaata tgacaacact tgaaaaggaa gtagtaaaaa gactgaagtt
     4861 tcagagagcg catggctggt gagaatgtgc acggatgctt ttgaactcgc ctttgagttg
     4921 cccttgctga atacccgagt aagcaagcgg cggtgacggc cgttacaacg ttcgaggcct
     4981 tttcactaag gtaaaattag gtggtaccgc gtagattacg tcctataaaa agagcaatct
     5041 ttttatagga tttttttatt tcttttgtga agtttgagaa aacaaagcaa aaggaaataa
     5101 aaataaacag attctgccgg ttttttcgtt atatttataa tagaaacaag tgttttcaat
     5161 agaacaggag gacagcaaat gagttacaat cataaagaaa ttgagaaaaa atggcaaaaa
     5221 tactgggcaa aaacgaatac tttcaacacc catgatgatc ctgaaaagcc aaaattttac
     5281 gcactggata tgtttcccta tccatccggt caagggctac atgtagggca tcctgaaggt
     5341 tatacggcaa cggatatcct ttcacgtgtg aaaagaagtc aaggatacaa cgtgctgcat
     5401 ccaatggggt gggatgcatt cggtctgcct gctgaacaat atgcgctaga tacaggaaac
     5461 gatccagctg agtttaccca aaaaaatatc gaaacattcc gacgccaaat caattcttta
     5521 ggatttagtt atgactggaa tcgcgaagtg aatacaactg atccggagta ttataaatgg
     5581 acacaatgga tctttacgaa gctttatgaa aaaggtctag cttatgaagc agaagtagcg
     5641 gtaaactggg tacctgaatt aggaacagtt atttcaaatg aagaagtgat cgatgggaaa
     5701 agtgaacgtg gtggctatga tgtagtcaga aaaccaatgc gccaatggat gctgaaaatc
     5761 actgcttatg ctgatcgctt attggatgat ctagaattag ttgattggcc agaaagcatt
     5821 aaagaaatgc aaagaaattg gatcggtcgt tcagtaggag caaatattga atttaaagtg
     5881 gctggtacgg ataaatccta taccgtattt acgacacgac cagatactct attcggtgca
     5941 acttactcgg ttcttgcacc tgaactagat cttgttagag aaattacgac ccctgaacaa
     6001 aaagctgctg tagaagctta tatcgaagag acagctaaaa aatcagatct aaagagaacg
     6061 gatcttgcaa aagaaaaaac aggagtattt actggtgctt atgcaatcaa tccagttaat
     6121 ggcaaggaaa tcccaatctg gatcgccgat tatgtattat cttcatacgg cacaggagcc
     6181 atcatggctg ttccagcaca tgatgaacgt gactttgaat tcgctcaaac tttcggacta
     6241 gaaattcttc cagttatcga aggtggagat gttgaaaaag ctgcttacac agaagatggt
     6301 acgcacatca actctgaatt tctagacggc atgaacaaac aagaagcaat cgataaaatg
     6361 aatgaatggc tagaagaaaa tggcgtaggg aaaaaagaag tcagctatcg cttacgtgac
     6421 tggctattct cccgtcaacg ttactggggt gaaccaatcc caatcatcca ttgggaggac
     6481 ggaacagtca cagctgtccc agaagaagag ctaccattac ggttgcctaa gacaaaaaac
     6541 atcaagccaa gcggtactgg agaatctcca ttagcaaata tcgaggagtg ggtcaatgtc
     6601 gttgatcctg taacaggtaa aaaaggccgt cgcgaaacga atacaatgcc acaatgggca
     6661 ggaagctcat ggtattacct gcgttatatc gaccctcata acaaaaaaga actagcagat
     6721 tatgaaaagt tgaagcgttg gctaccagtt gacatctaca ttggtggagc agaacatgcg
     6781 gtacttcatt tactatatgc acgattctgg cataaattct tatacgatat cggtgttgtt
     6841 cctacaaaag aaccattcca aaaactatac aatcaaggaa tgatcttagg cgaaaacaac
     6901 gaaaaaatgt caaaatctcg tggtaatgta gtcaatcctg atgatgtggt cgaaacttac
     6961 ggtgcagata cactgcgcat gtacgaaatg ttcatggggc cattagatgc ttcgattgct
     7021 tggagcgaaa atggcttaga aggaagccgt aaattcttgg atcgtgtatg gcgtttgatc
     7081 gttgatgaga acaataaaat gcgtgaccgt atcactacat tgaatgatgg caagcttgat
     7141 aaagtgtatc atcaaacagt gaaaaaagtg acagaagact atgaaaatct tcatttcaat
     7201 acagcaatct cccaattgat ggtctttatc aatgaagcct ataaagtgga cgctctgcct
     7261 tatgaatata tcgaaggatt tgttcaacta ctcgcaccga ttgctcctca catgggtgaa
     7321 gaactatggg caatccttgg aaatgatggt ggtatttctt acgcaccatg gccaacatac
     7381 gatgaagcag cacttgttga agacgaagta gaagttgttt tccaagtaaa cggtaaagtt
     7441 cgtgcgaaaa gcaatgttcc ccgagactta ggaaaagatg agctagaaaa agcagcatta
     7501 gccaatgaaa tagtgcaaga atatattgaa ggcaaaacag tcagaaaagt tattgcggta
     7561 ccaaacaagt tagtcaatat cgttgctaat taattggctc tttgtcaata aggactgatg
     7621 agttgtgcaa aatcaaatct gggccagaat gaacttcatt ctgtctcaga ttttttgtta
     7681 tttcaaattg attaattgat tgatcaaaaa ggttttgatt ttcatgttct caaacacgtt
     7741 caatgtcttt ttcttctgaa tggtcttgta agagatctct gtaatcatca aatttttgtc
     7801 tattaatctt agtatttttt gatgtataaa ctacctgaaa ttgagagtac ataccaaatt
     7861 cataaatttt ttattagtgg gactagtgat tgtatggatt ttgacgcata atgaatgaag
     7921 tatgagaaaa ggagagacaa atggatcaag aactatttat tatcgaagta ttatcagcta
     7981 aacaaaaaat tatcgagaaa gcgaaaaaaa gagcgaaaag aataatgttc aagcttttaa
     8041 gcttagtatt tttctctggt ttgctttttt tcggagctgt atcgttccta tatcgccata
     8101 tgagtgcagt agtccgttca gctgaaggga tgcaaaaatt aaataatttg tatcactttt
     8161 cgtggggagt tggcggaata acaattgttg tgcttattgt ttttcttcta cttatttggc
     8221 tccgttacca tcaattgtcg cgtcatttcg atcaatggct tagtcaaaga gcacaacagt
     8281 ttttacataa taaaccaact tctcaagatg atgactatct ttattttgag aagacttcga
     8341 cgagtcaagc ttttcggata caaaaaagtg attgccagtt attgctgaga tcacttgatg
     8401 atttacctat ctacttaggc gaaagcaaat caaaattcgg aatgaacaca ttccatattt
     8461 ttttactaga aaacgaaccc agatgggaaa aaagccagtc ttcgcctggt ggatacctga
     8521 caactaagat gagtacatta gtcactggtt ttttcctcat tcttttagct ggaacaatct
     8581 attatgggag atacactcag ccaaaaatgt cttctcatat aaatgctgct tctcacatga
     8641 caatgaactc aactaacgat acgcatatac aaaagacaaa tgaagggttg ttagctgatc
     8701 ctaagcaaaa ggtaaatttg acaacacaaa agataaatga tctcaaactt gatacagaga
     8761 cgcactctct ctatatgaca acagatagtg gtgctaattg ggtatttgtg ccgattgatt
     8821 cgcaatggtt gcgttttggc gaatacaccc tgacaacggg gctgattcct gaaggttatt
     8881 ggatggacaa cacatatgat atttccgccg atttctcatg gtttatctat tcttctgggg
     8941 atgatgtacg tctgctgact tcaagagata atggtaaaac atggcaaacg tctattatca
     9001 cccaatcagt tcagccgata cgttatcgaa aagcggtatt ttatggcaac gatggtctat
     9061 ttatctgtac atcagccagt gggatgtctg cagaaacttt gtacatttac actactatgg
     9121 atggcggaat cacttggata aaaagtggaa gtacaatgat caatcagcca gtacaaaatg
     9181 tcagcttcgt tacaacttct ttaggttttg tttcgacacg tgaaagtttg ttttatacaa
     9241 acaacggagg acgctcattc aaagaatcag ttctgtcgat tccggaaaac tatcagacag
     9301 gggggataga tctttttcaa tcaccaaatg aagttactca aataagcgcg aatacgttag
     9361 agacaaaatt ttatttgttg aaaaaaggac caatcgatgc agggaaaatg tttgcctgcc
     9421 tcttccgttc aactgataac ggggaaacat ggcaattcgt tcagcaacta tcgcaagtag
     9481 aacaaggaga ttaaagaaga gagattgtga gcctgacgtt cagcaccgag tcacaagaaa
     9541 caaattcaaa aaacagcttc tcatatttgt tgaatagtgt aactcgtgat tcggagttga
     9601 caagagcaac aagaagcgat tttgaagtat agcttctcgt attttacaag atcgtgtctt
     9661 attgccgata ggttgtctct tgaacaccgt ttattcgtaa aaagggattg tgacaagtct
     9721 tttgtcacta cttaccgaac gaaactgaag gagctatcca aaaagtatca ttagtattat
     9781 tctttgtgtt tctagtggca ccactagtac atttaaaaaa gctatcgatc atggactaat
     9841 catacatgat cgatagcttt ttagataatg tttgttacgc tatctgaaat tgactattat
     9901 aaagactggc ataaagcgtt ggtgattgga gtagttcaga atgtgttcct ttttcgacga
     9961 tatcgccatt cttcattaca aggatcatgt ctgcattttc aatcgttgac aaacggtgtg
    10021 caatcacaaa acttgtccta tcttttgtca cagcttccat tgcttgctga atcaatgctt
    10081 ctgttcgtgt atctacgcta gaagttgctt catctaaaat aacgacagga ggattcgcta
    10141 gaatgactcg agcaattgtc aataattgtt gctgtccttg agataacgtg ccgttttcgc
    10201 tagaaatgat cgtatcatat ccgtctggca gtgttcgaat aaagtgatca cattgtgcga
    10261 tttcagccgc ttcgataatt tgttctcttg tggcatccat tttcccgtag gcgatattat
    10321 cagctaccgt tccttcaaac agccaggtat tttgtaaaac catcccaaaa gaacgacgta
    10381 gttctccacg tgaaagcttg gtaatatcaa gaccgtcgaa ttcgatcttt cctttattca
    10441 gttcataaaa acgcatcagc agattgacta aagtcgtttt tcctgcacca gttggaccaa
    10501 cgatcgctac cgtttgtttt ggctttactg aaaaatcgac ttgattcatc aatattttat
    10561 ctgatgtgta accaaactgt acatttttga aagctatttt tcctttcggt tgttctaaaa
    10621 gtcttgcatc tggaaggtct ggaatttcat ctgtttcatc taatatctca aatatcctat
    10681 cgattgctgc cattgcggat tggatcgagt tgatcacata agaagcggta gaaattggtt
    10741 cagaaatctg attgatgtac tgaagataag cttgtaagaa gccgatcgtc acaccacctg
    10801 atagaacaag catagcaccg aataccgcac ttatgatgaa ggcgagttga ttaatgaaac
    10861 gaatggccgg atagatagca cagttcataa attgagcacg tttaaaagcg ttgtattgct
    10921 ttttatttac ttcgtgtata gcagcttctg catgagcttg ctgattgaat gttttgacca
    10981 ctagattacc agctaagatt tcttccattc gattatttaa aacacccagt tcatactgat
    11041 tctgttcaga aatctgtttg tttttgttcg caatcttagt tgtaacaatt gaactgccaa
    11101 cgatcagcaa taagacgagc aaagtaagct tgctttcgat ataaaacagc atcagaccag
    11161 cagcagctat tgttacgaca gaagtaaaaa attgattgat acctgtcaat aacacttgag
    11221 aaagttgatt gagaccggta gttgtccgac taatgatgtc acctacttga tgattgtcaa
    11281 aaaacgacat cggtaatgtt ttgaattttt ttgtgatttc ttttcgcacc ctcaatgtga
    11341 tcttttcgct taacgctgcc attgtccgtt cctgaataaa tgaagtgatg ctgcttaaga
    11401 ctgaacagcc gatcagcaat aagacaggga acaacaatgc ttgtttgata tcactgaaag
    11461 aaagctgtcc aatacctgtt tttcttattt ctgataagag atcatcgatc ccgattccca
    11521 tgatcaaggg catggctacg actaataggt tcccgaataa actgcataga aacaatccgt
    11581 aaaaatattt atgttcaggt ttgatcattt tccaaaaacg tatcaaagca cgcgtgttca
    11641 acgttttttt cttttccatc gtatcccttc tttctatatt ccttgtgatt ttgcaaactc
    11701 ctgataagtt tggcaattag acagtagttc ttcatgagta ccttgtccaa cgatttttcc
    11761 ttcatctaaa acaatgatgt tatctgcttg ttggattgta ctgaggcgtt gagcaacaat
    11821 cacgagagtt ttctccgaca tgtgtttatg aagggctgct cttaatgctg catctgtttt
    11881 atagtctaaa gcggagaaac tatcatcgaa tacatagatc tctgctggtt tgatcaacgc
    11941 tcgagcaata catatccgct gtttctggcc ccctgaataa ttagaaccgc cttgagcaac
    12001 aaagctgtcc aatccgtcag gaagggtgtc cactagttct tctaattgag cgatcttgat
    12061 cgctttagac aactcttctg ttgaggcata ttcatttccc atctgcaagt tgcttaaaat
    12121 tgtcccgctg aataagaaag ccttctgagg gacataactg atgtgggagc ggatctgttc
    12181 ttgagtcatt tgagtaagag gaataccacc aagggttact tttcctatcg tagggtcact
    12241 aagtctcaag agaagtttcg ccaaactact ttttccagat ccggtcccgc caacgatcgc
    12301 tgttgttttt cctttaggta ttgtgaaatc aatatcttct agaactggat cagagatttc
    12361 agtatattta aatgtgacat gttcaaaagc agctgcattt gtctgatcaa aggttggttc
    12421 agctactgtt ttgatcgtct cttcctcaat ttcgatctct gcttgcagta tttcttctat
    12481 ccgtcgtaat gaggcaaacg atttagggat aatgaccaat accatagctg cgatcatcaa
    12541 gtaagcaagt gtcagcatag agtattcgac tacagcagtc acgacaccga tttgtaatgc
    12601 tccttctgct acaagatttc cgccaaacca caaaatagaa gcataaacga ttcccatgag
    12661 caaaaaagca attggagtga cccaagcaaa tattcggttc actttgatca tttgattagc
    12721 atagtcagca aagctcttat tcgttcgctt ttcttcatat gattgattat caaaggcacg
    12781 gatcatatta atcccagtaa agaattctct caatgtgatg gtgatccgat ccatttttgg
    12841 ctggatactc aaagataaag gtgcccctcg tttcatcagt cgataaacaa tccatacata
    12901 aaaaacaaca gagatcaatg taacccatgt tagtaataga gaataacgaa acatcatgag
    12961 aatggtgaaa acgcaaataa ccggtgctgg taagatcatt tggcaaaata aaacaatcat
    13021 ttgctggaca ttatcgacat cattcgtcat tcttgtcaaa agagatccag aactaaaacg
    13081 actgatatcc ttcattgaca gctgttggaa tttacggaaa tatttctccc gcgtttggaa
    13141 tccaaaccta gcggaagcca atgctgaaaa gtaactagag gcaactgcag cgattgttcc
    13201 tagaaacgct acaagcagca tttcaactcc aatttttttt ataactgagg cttcgccact
    13261 tgctatacct gtgtcgatca gctttgctac taattttggt acacctaatg taccggcgat
    13321 ttctaaacag atcatcacaa aggtaaggag agcgagtcct ttgtttttct gtaagaatgt
    13381 ttttaagata gacatattat tcctcctttt ttcctgatac ttttcgattc tataccttca
    13441 agtaacttga aggtcaatag aaaaaagaat aaactgaaaa agaggtgaga aaaaatgaaa
    13501 aaaatgtata cgattggcca agtatcggaa ctatttgatt tgccaaaatc aacgatccgc
    13561 tattgggatg aacaaggatt aatccgttca agtagacaag aagagaatga ttatcgattg
    13621 tttgatatcg atgatatttt tatgctttat gatatcgttt tttatagaaa attagatata
    13681 ccgatcaaac agatgaagaa tttatatgga aaaacattga cggagttata tgagacactt
    13741 gatgaaacag aaagaagaat ccataaagaa ctggtggtga tgaaacaaaa acaaaaagag
    13801 ataagggagc aaaaaaaaca gttgaaattg atgatcgata caaacgagga agaatttcct
    13861 gtagaagaga tcccttttga ctgtatgatc tcaacggagt ttgaagatat tgtggaaatc
    13921 aaaaaatttc tacctaacta ttcaagcttt gggatgatga gtatgccagc ttccagttca
    13981 cagacgatgt atggattttt tatcgaccct agtgaagtac atttatttac tcaagacgtg
    14041 atttgggaga aaaaagatac ggctgtttat cgtcgattct tattgaaatc cgagatgaac
    14101 catgcggaac gtaataatat tttagaaatc agagaaagaa tgtatgaaaa aggatggaaa
    14161 acaggagaag tgatcggcca gtatctattg acgaatacgg atgaaaacaa tatcagaact
    14221 gaatattatc atgcatggat agaaatgaaa aaataggaaa tatgtgttta agataactcg
    14281 aaaatgatgg ttgttttagt tataaagaaa aattttctgg ttcgattacg ggtttaatgt
    14341 gggggattat tagtcgtgga tagatagaat tttagttttt tagaggaaat atgtctttaa
    14401 aaatggaatg gttgcaattc ttagcgatat atacgatgta attgtcctct taaaaaagta
    14461 tggaaaaata attgtattgg gtgttatgta taattcatgg tgaactgaga ctaatgagaa
    14521 gaaaaattca tattgttatg agtgagattg tatcagatga gaatgataaa ttaaataggt
    14581 gcataaaagc attacactta aaatacattg ttgcttacat tattacaata tgttaataag
    14641 gcaaaaataa atagatggat tgaataaaca tattttttaa tcaaatagtc cctactgtga
    14701 ctttcctcag ctatcggtga tactaccgat agcatttttt tgcttataaa ataattataa
    14761 gtatttagag atttttcttc taacattctt caaacaagaa aaatcacaaa ggttattgtt
    14821 atttttatat attcttttta agaatgattg tttttttata gaaattttaa aaaataaaac
    14881 acgttattga tggatagtta gtttgttatt tggtaatatt atagaagttg actggtagtg
    14941 atttattttt cgtcgtttta taaaaatgtt attttttgga aaaattcaca aatatgtaac
    15001 gaatgttttt ctggaatttg ctgtgttaaa aagcagtatg actaatataa atactattgt
    15061 catttatgca attttttatg gatagtaatt ttagagggga atcaatgaag gaagaataga
    15121 agggaaaaat agttgcaaat acgccagtta gcaaaaatag caatacatct tcaagcttta
    15181 aaattcgaga gaatcatctt ggtagtggaa tcaactacaa caaaaggatt aaaccaatta
    15241 atagatagaa ttaccaatgg aaaaatagaa aaggtagtta ttctatacaa aagttgttta
    15301 attcgcttcg gttatgaact tagtgaaacc ctttgcaata tatggtacag aatatagaaa
    15361 ttattgacat tacagaaaat gatgaagatt taatacagat tgtcactatt tgtagatacc
    15421 ttttacaagg aaaccgagcc aataaagcta agaagatgat taaggagttg atggaagacg
    15481 atacaagtta agaaagtgaa actacgccca aataaagagc aaaaagagta attggacgtg
    15541 gaaaatgcaa gaaatgaacc atcgattcgg tggacagttt attagtaatg acggtttgag
    15601 aacatccatg acgagaatga aggaacatcc taaatgcgtt tagttgaacc aagtgccaaa
    15661 taatatggcc aaataagcga tcagttatcg atgaatccca agtattctaa actatatatt
    15721 cattttgatg ctaagcattg atatattttt ttgggattga tgaaggttaa ccaatacagg
    15781 gactcactga tgaaattcta gagtctgacc ttggtatcaa cgatttagtg attgtttcta
    15841 atggaaaagt ataaaagtat ataagtacat taacaaaata actattaggg ttacgctcca
    15901 gcttgtgcaa ggcactggca ggaggaatcg tgaaatcata attatggtta agccacacgg
    15961 acaactaaaa gtcatacaaa gctatcgaat caatcaacag aagacaatcg gcaatcatta
    16021 gctttgttta cagcttacat tgcaacgctt gagttgaacc gaaaatacga gggaggtaac
    16081 gcagccattt tgagttgggg aatcttttta gaaaattatc gggtaccttg ttgtttagtc
    16141 aaaattataa gggggagtaa tagtgaggaa caaatttcat aaactgttaa gtatgtttct
    16201 agttatgctt attgtgatca atcaatttgg gaacataacg gttctagctg agtctttagc
    16261 agaacagcca gagcgttcgc tgacgatcga agttgatcaa gaggaggctg ctttgggtga
    16321 gcagttccag ttgcgagtca gaggaacacc agacctgcta aatgcattgg tcattgataa
    16381 gagtgaggat ttgattgaag gggaagtgaa gcaactcaat gattacgaac gtctgattac
    16441 ttattcttcc cagcattcag gtaaatacga attgaccgca agaagcggtg atattcaatc
    16501 aaatacggtc tcaatagaaa tcagtgtacc agaagaaagc acaagtgagt cttttaatgt
    16561 accagaagaa agcacaagtg aatcttttaa tataccagaa gaaagcttaa gtgaatcttt
    16621 taatgaagcc agatcatctg agccatctat aacgcaggaa tctcagattg aaagacaaga
    16681 ggagctcttg aattatgaca tcggcatctc tgctgagcgc cccactttgc cgagcaggca
    16741 tacacaaacg ttaaagggat acattcaagg aggcgctggc gatattcttg aagaatatgg
    16801 ggagatggtc gtacgagttc ctagagcatc gattggctta attgagttca ttagagtgga
    16861 ggtcgctgat gattcacctt taaagccact ggcattttac caagaaggca cagacgttgt
    16921 ttttccattt acctttgatt atgaaggatt aaatgaagcc gctcagtcgt atacattcag
    16981 tatcacgttt gaagttgctt tctttcaatc aggcggtact gagccaaata atgactttca
    17041 agtgagtctt acttatgctg agcgagcagc aaacacggaa ttttcaatta cgcctattcc
    17101 ttcatcaaga cccatttttt cgaaattata ctatggctct agagaaatcg acagccaagg
    17161 aaatcttggg aatctgctga ttgacgggca agacgtgacc aacagtaata aatttatttt
    17221 gcaggtaaat tatgaaggat cacgaaaaga cggtgtagtg gttaccgatg tactaccgca
    17281 aggaacaact ttggcaccga atccgactac atggagttcc actggaaata gagaacctat
    17341 ttcaggtggg gttcggatcg cagaagtaac gaatccaggt gaagcaaacg ttagaactca
    17401 ttatgtttcc gaggaatttg tggacaaagt atcttttaat aaggaaacga atacacttgt
    17461 tgtggacttt ggtaatctag cagagggtca atcgtatttt attgaatatg ctcttgaagt
    17521 gaccgatgtc gacttgttca atgaaggaag acaagaggat gggttttctc ggaatcgagc
    17581 gagtatgacc tacaataatc aggtaatgag ccgagactac cgcttgagaa tccctgaaag
    17641 taatcttccg gcaatctctt taagaaaaag tgtggacaaa aatgttatca atctcaatga
    17701 aaatgaacta acgtatactc tgcatgtctc gatgttttct ggaacaagaa atacgcttgt
    17761 tattgaagat gttttggaag aaggcatgga atttgtgtca tttctatcag atattccctc
    17821 tttcattgat gccgaagtca ataatggaat agttcgtttc agtgcgaata atacgattgg
    17881 tggtccgaca agttttaccc tctcttttaa ggtcaatgta gaggcgtatg cggtcggcga
    17941 tcaaatttta aactttgcaa cgcttgtgaa tagtgacaat cgattgaata cgagtactgt
    18001 aagaacaagg aaaatcgatg ggcgtattaa agtaattaaa actgatgaga atggccaaag
    18061 gctggcagga gcaaaatttg aaattttatc aagcgattca caagtgatac aagaaggtat
    18121 aacggcagaa aacggagaat ttttaagtga accgcttcct gtaggtagct atcaagtccg
    18181 agaaacagaa gcaccggaag ggtatctttt agatgcgacg gtacatgatg tttttataaa
    18241 tgaatactcc atagaagaag tgacgttaac cattgaaaac atccttgata cgggtggtgt
    18301 tgaactaatt aaacatgctg aaggcagtgg tgaagtcctt caaggagctg tttttgagtt
    18361 acagaaccga gaaggagaaa cgctacaaac cggt
//
LOCUS       DAFGZE010000055        18104 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.057, whole genome shotgun sequence.
ACCESSION   DAFGZE010000055 DAFGZE010000000
VERSION     DAFGZE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.057"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(164..388)
                     /locus_tag="LTU54_002026"
     CDS             complement(164..388)
                     /locus_tag="LTU54_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HBL8296731.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(586..2166)
                     /locus_tag="LTU54_002027"
     CDS             complement(586..2166)
                     /locus_tag="LTU54_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBL8296732.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(2567..3943)
                     /locus_tag="LTU54_002028"
     CDS             complement(2567..3943)
                     /locus_tag="LTU54_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBL8296733.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(4071..5189)
                     /locus_tag="LTU54_002029"
     CDS             complement(4071..5189)
                     /locus_tag="LTU54_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBL8296734.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            5331..5765
                     /locus_tag="LTU54_002030"
     CDS             5331..5765
                     /locus_tag="LTU54_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8296735.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(5843..6376)
                     /locus_tag="LTU54_002031"
     CDS             complement(5843..6376)
                     /locus_tag="LTU54_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HBL8296736.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LTU54_002032"
     CDS             complement(6470..7648)
                     /gene="mutY"
                     /locus_tag="LTU54_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBL8296737.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LTU54_002033"
     CDS             complement(7641..8438)
                     /gene="recX"
                     /locus_tag="LTU54_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBL8296738.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            8545..9927
                     /gene="rlmD"
                     /locus_tag="LTU54_002034"
     CDS             8545..9927
                     /gene="rlmD"
                     /locus_tag="LTU54_002034"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBL8296739.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(10101..10679)
                     /locus_tag="LTU54_002035"
     CDS             complement(10101..10679)
                     /locus_tag="LTU54_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HBL8296740.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            11001..11471
                     /locus_tag="LTU54_002036"
     CDS             11001..11471
                     /locus_tag="LTU54_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBL8296741.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(11579..12805)
                     /locus_tag="LTU54_002037"
     CDS             complement(11579..12805)
                     /locus_tag="LTU54_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBL8296742.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(13559..13759)
                     /locus_tag="LTU54_002038"
     CDS             complement(13559..13759)
                     /locus_tag="LTU54_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL8296743.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(13893..14501)
                     /locus_tag="LTU54_002039"
     CDS             complement(13893..14501)
                     /locus_tag="LTU54_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBL8296744.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(14586..15056)
                     /locus_tag="LTU54_002040"
     CDS             complement(14586..15056)
                     /locus_tag="LTU54_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBL8296745.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(15104..16018)
                     /locus_tag="LTU54_002041"
     CDS             complement(15104..16018)
                     /locus_tag="LTU54_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL8296746.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            16164..16967
                     /locus_tag="LTU54_002042"
     CDS             16164..16967
                     /locus_tag="LTU54_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HBL8296747.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            17145..18092
                     /locus_tag="LTU54_002043"
     CDS             17145..18092
                     /locus_tag="LTU54_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBL8296748.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         5436 a         3631 c         2964 g         6073 t
ORIGIN      
        1 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcgaaagca gaagcaactc
       61 cttcggaaat aagctgaaat tcacgaaaat tgcttttcaa atttttgtga attccagctt
      121 atttcttgga gttaaacgct tctgtctcag cctctttttc tttctagctt tttgattttt
      181 ctcttttctt ttgtactttt ctcttttcat tttcagttct acgcgtgtct ttatttttaa
      241 ctttttcctc tttatggatg attgatcgta ctttttgtgc aatttgctcg tttttgtccc
      301 agtcttttac atctaataat cgacataatc gtaacatctc ttcttttttc tgtttttttc
      361 tttcacgtct ttgccattct gtttccatgt gtcacactct cctttcttta atcatatgta
      421 cgcaaaataa tttttatatt tttttgatta gacatttcta tgattttaat atagtaaaaa
      481 gagccgtcac ggaaaatccg tcacggctct aaacaaaata tgagaggctg tttttgtttg
      541 gctattctta ttacttgatg cagggcagct tgtgtcaaga cttctttaca tcaaactttt
      601 taattttaca ttgggatatt tatccgcaaa ccagcgttct gcaaattggt tctcgaataa
      661 gaacaacggt tggtcgaaac ggtcacgagc caagatattg cgacttgaac tcatcttctc
      721 atccaaatct tctggatcga tccagcgagc gattttgtgt cccattggag acatgattac
      781 ttcggcgtta tactcattta acatacgata ttggaatact tcgaattgta gctgaccaac
      841 agctccaatg atatattctt ccgttagata tgttttataa agctgaatcg ctccttcttg
      901 aactaattga tagattcctt tgtggaagga tttttgtttc atgacatttt ttgcagtcac
      961 tttcataaac agctctggag taaatgaagg gagttcttca taagcaactt ttagttttcc
     1021 ttcgaacaat gtatccccaa tctgataatt gcctgtatca tatacaccga tgatatcgcc
     1081 tgctactgct ttttcgacgt tttctctcga atctgccata aattgtgtga cattgcttaa
     1141 tttcagtttt ttatttgtac gttccaacca gacatccatt cctcgttcga atgttccaga
     1201 acagattcgg acaaaagcga ttcggtcacg atgggctgga ttcatatttg cttggatctt
     1261 gaatacgaaa cctgaaaatt cttcctcata aggacttacc atctgatcat cttgtgtttt
     1321 gtgtccataa ggagctggtg ccaattcaac gaaagtctct aaaaaagtct gtacaccgaa
     1381 gttagtcaat gcagaaccaa aaaatactgg tgtctgatcg ccacgagcaa ttttttcttg
     1441 attaaatgca tctccagcct ctttgattag ttctacttct tctaagactt gttcatataa
     1501 ggaattttca gttagaggat gattttcggg aagcagtcca tcttcatcca attctactaa
     1561 tcgttcatcc tgataatttt ccggacgata aaactctaca cgattatggt agatatcata
     1621 aagaccttct agccctttac ccataccgat tggccaattc atcggataag attcgatatc
     1681 taatagctct tctaattctt ctaacaattc caatggttca cgaccatcac gatctagttt
     1741 gttgataaaa gtaaatatcg ggatgccacg tttttttaca acttggaata atttctttgt
     1801 ctgtgcttcg atcccttttg cactatcgat taccatgacc gcactatcca cggccatcaa
     1861 agtacgataa gtatcttcag aaaaatcttc atgtcctggt gtatccaaga tatttactcg
     1921 tttcccttga taatcaaact gcataacaga actagtaaca gaaatcccac gttgtttctc
     1981 gatttccatc cagtctgatt tggcaaaatt acctgttttt ttgcctttta cggtcccggc
     2041 ttgacggata gccccaccaa acaatagtaa ttgttctgta atcgttgttt tcccagcatc
     2101 cggatgggaa atgatcgcaa aagttctgcg attgtccact tgttgcttta attttggatt
     2161 attcataacc taactccatt tcttccattc aattatttat attttctcta ggcacagatt
     2221 gtccgatctc ttaaagaaag acaatttcat atagggctgt gacaaaagcc gtgtagcagt
     2281 ctctttttcc gagtaaacgg cgttctagag acagcctatc agcaataagc ccacccaagc
     2341 caaaaatacg aaaagctgtt tttgcttggc tgctcttatt acttaatgca ggatagcttt
     2401 ttcacaacct ttgtcaacta ctacaactga atcagtatag catggattca gcgaatgaaa
     2461 aagccaccaa accataagag tcaaatattt ttttgtcagc tgactaaaag aaaagacgtg
     2521 ttgcttgttt attcaagcaa cacgtctttc ataaccaagt ttccaattat cttcttggct
     2581 cgtcatcttc gaattctttt ctgaagtatc ttcgttcttc ttcaggttcc gcttctgttt
     2641 cttccggatc atgaaaaatg acacggattt taaggattcg agaaccttcc atttcttcag
     2701 aaaccagcgt aatattatcg acatcaaacg ataacttttc gccttcatcc ggaatcatgc
     2761 ctaatgctgt aatcaaataa ccagccatcg tatctacatc actcatatgc agatcactgt
     2821 caaaagcttc attgaactca tcaatcagca tacgtccttg gatgatatat tcgtgttcat
     2881 cgattttttc ataaagattc tcaacttcat ctgattcatc atcgatttct ccgacgattt
     2941 cttctaaaag gtcttctagt gttgctaacc caacgacgcc gccatattca tctaataaaa
     3001 tagccatctg attctgcgtc tttttcattt catataacag atcatcaata aagaccgttt
     3061 caggaacgaa taaaggctcc tgcatgattt tcttgatatc taaattatca aagccgaatt
     3121 tatgggcagc ttttagtaaa ttctttgtat gaaggattcc gactacttta tctttgtctt
     3181 cgttataaac agggattctt gaatagtttt cggataaaac ttcattcaca ttttcttcca
     3241 cagtgtcgtt gatatcaatc ataaatgcat ccgttcgtgg gaccatgact tctcgggcaa
     3301 ctttcgtatc aagagaaaaa accccttgga gcatttctaa ttcttcatta ttcaatacgc
     3361 cttcgttttc aagcatataa cgcatttcat ctctggtcat ttttgaatct tcatcatcaa
     3421 aagtcatcgg cgtgatacgc gccaataaac ttgtggaagc agaaagcagc cagacgaagg
     3481 gcttagctac tacaccaatc aagcgtatca gccctgaagt gaactttgcg acttcttctg
     3541 atttatttaa agcaatacgt ttagggtaca attctccaaa aacaatcgat acataggtca
     3601 aaatagccag cacaatgata tttgcaatat tttttgccgc tgtgccgcca cctaataccg
     3661 gagctaaccg ttccgataaa gtattagcta gtgaagcacc agacaaaatg ttgaccaaag
     3721 tgattcctac ttggattgtc gataggaaat tatttggatt ctgcaatatt tttaaaagct
     3781 tttgagattt cacatctcct tcatcagctt tttgctcgat tcgattctta ttgatcgaaa
     3841 caacagcaat ttcggaagct gctaaaaaag cattgatcaa tgtcaaaacg atcaataata
     3901 aaatctgcgc taacagcgac tgactctcag ggtcagcatt cataatcgca ttctctcctt
     3961 ataaaattaa tttttagttt acgatagaaa aaaacgtggc actatagcca cgttaaaatg
     4021 gtatcacaaa gcaattttct ttgcaataaa aacaagtgta ctaaaactac ctacgaggtc
     4081 gtagcaattt tcttcttgtt ttcattttgc ttgtcttctt tgacgatttt gattatatag
     4141 gaaatgatcg tcttgcagat tgcgtaaaaa ggaacaccta agaagatacc aagcaatccg
     4201 gcaatatttc cagctactag aagaacgata atgatcgtta atggatggat tttcagtgat
     4261 ttcccaatta cattaggata aatgatattc ccatccaact gttggacgat tagcacaacc
     4321 acacaacaaa gtaatgcttt taccggatca ttgaagactg ttactagaaa tgcaggtgct
     4381 agtcctagat aaggtcctaa atatggaatt aggtttgtca atcccgcaat gaccccaaat
     4441 aagaaagcat aatccacacc aattaccata tatcctaaaa atgtgaaggt accaacgaat
     4501 aaacattcga ttgcttgtcc gctaatgtaa ttagccaatg ttttattcag ttgccccaat
     4561 aactccacaa tttggtttct tcttttttct gggaaaaact gttggatgtt tggcactagt
     4621 ttttctccgt ctttcaacat atagaacaag atgaatggtg ctgtaatgat tactacggtt
     4681 gccgaagcga tcgttccaac gatcgaacct aaactattgg atactccact aaggaattgc
     4741 tgaatgattg tcccgtaaga aatgtctaat tgctcaaaat atttattgat atctacttcc
     4801 ttgaacaaag gcaattcggc tacctctttt aaccagcttt ctacctgttt gatgaaatca
     4861 ggcatattag atgccaatga ggaaatttgc tgtactagac ttgggatgac actaagtaaa
     4921 atccaaataa ttgctgcaac taaaaggagc agcacaattg cgacagccca tattcttttg
     4981 atttttgctt tcatcaacag cttgacaata ggattcaata aatagtaaag aaagcctgca
     5041 atcagtacag gtgcaaataa cgtggaaaaa aatgtcccga tcggttcgaa aataaagttt
     5101 atttctgatg aaatcatgat caatgcagct atgatcaata attcaagcga ccaaaacatt
     5161 agtttagact gtctaagttt ttcaaacaat agatttcctc ctttctttct tactagtata
     5221 tattatattc gttaaaaatt cttttattct ttttttattt ttgaaaactt gccatacttt
     5281 ttatttgttt tattttccag tatactatac gaaaggaagg tggctagacc atgaaagttg
     5341 tacaaacaaa agatacaatg agcaatattt acttagatgc tgtacggatc aggcatcaag
     5401 tttttgtagt cgaacagggt gtgcctttgt ctcgagaaat agacaaagat gaagcccatt
     5461 gtatccactt cgtcctgtac tcagataaaa aagaaccgca aggaactgta cgtcttttgc
     5521 ctttagaaaa cggaaaaatg aaattacagc ggatggcgat tctttctgaa tacagacacc
     5581 aagggttagg aaaaatattg attgaagaag cagaaaactt tgctaagaat caaggctata
     5641 atacgatttt attaggggca cagtccacag ctgaaacttt ttacgaaaaa ctaggatata
     5701 cagcttacgg cgatcctttt gaagatgctg gcatgcctca tatttatatg aaaaagactt
     5761 tataaaaatt tatttcgaaa aaagcaaaaa acagcaagct tttgagaaaa atctctcact
     5821 ggcttactgt tcttttttgt gtttacttac gcgacaattg cttgtaacgg ttataccaaa
     5881 tatcaatata gccttccgag aacgggccat ctccattatt gatccagtca accagaatct
     5941 tcacattttc ttttaggatg aagtcaatat cgttcgggta attcatcatt ttgctgtgca
     6001 tctcatattc atcgacatcc aatagtcgtt tctcgccgtc aggaaatacc ttgatatcca
     6061 agtcatagtc gatatatttc aaggcctcat cgtccagcaa aaaaggtgaa gctagattac
     6121 aataatagga aacccccttt tctctgatca ttgctattac attgaaccag tattttttat
     6181 gaaaataaac aatcgctggt tcgcgcgtta cccatcggcg tccatcagat tcagtgacta
     6241 atgtgtgatc attcacgccg atcatagagt gttcgcttgt ttttaatacc atggtatctc
     6301 gccacgttcg atgcaatttg ccgtcgtgct tgtaactttg gatcgtcaca aactctcctt
     6361 cttttggaac tcccatagca acccttcctt aagcagcatt ttgtgctttg tgtttgggtg
     6421 ttcattacac cgacatcata attattatat caataaaaat gagctttgcc tagaaaggat
     6481 tcctattgtt tcgatttttc tctaactgat ctaccagttt catttggacc ttaggaaaaa
     6541 cgaccgaatc aaatgcatca ggcttcaacc acttcgaagt tttgttctcg gtaaattctt
     6601 gttctgctcc ctcggttgcc cgtccgtaaa aaaggagaac atgccatttc agatgactaa
     6661 aaatatgagt aacttctcct aaatgtcgtg tctgccaaac aaagagttcc tgcttttcaa
     6721 atgggagatt ttgctcttct tctgctacta ggtcatcaaa cagatcgatt tctggctttg
     6781 tttcccattc ttttttcaat cgctcatact cctcttgtgt cacttccatc attggaaatg
     6841 tccacatatt cgccagcaat ttttggctat ctctttcttc aaaataatac gctccagaat
     6901 gattttgaag agctgcactg atataataaa cgtcttttgg ctttgctttt tttgttttta
     6961 caggaaaact cgtttggatt cctcgtttgt tagctaaaca aaaggcttgg atcggacaag
     7021 tctcacactt tggagaagtt ggtgtacaga tagctgaacc tagatccatc attgcttggt
     7081 tgaattcacc tggatgcttt tcatcaatga ttttccgcat cgcttcatca aagattttcc
     7141 tgctagaagc ttttgcgatg tctgcttcaa tgcaaaacaa tctgctgact actcgcatca
     7201 cgttgccgtc aactgcaggt tctggaagtc caaatgcgat acttgcaatc gcgcctgtcg
     7261 tatatggtcc tattcccttc aatgagctaa tttcttcagg cgtttgcggc atttccccat
     7321 caaattctga catgatctgt ttagcagcag cttggatgtt ccgggctctt gagtaataac
     7381 caaggccctc ccatgctttt aaaagctttt cttctggagc atttgctagc tcttcaattg
     7441 taggaaacca ttccatgaaa cgataaaaat aatcgatcac tgtgtctact ctggtttgct
     7501 gaagcatgat ctcagagatc cagatacgat aaggatcacg attatagcgc caaggcaggt
     7561 ttcttttttc ttgttcatac cattgaatga actgatcttg aaattctttt gtttctttat
     7621 ctgtccattt ttcccattct ttattcactt tcctcttcct tttccgtaat gaatcgctgg
     7681 atcatatcca aatcaaatcc tttttgatat aagctttgct ttacttttag atttcttttt
     7741 ttaggttcaa accttaaatt tcttcgccat agtttatctc cttgtttgac taaataatca
     7801 tattctattt cttgatctgc ttcttgcgga agatcagcta tcgcttgttg gatcacttct
     7861 tgagaaaagc ctttcgtcat caatgtttgt tggatttttc tcaataattc tttttggctt
     7921 tttccgtgat tttttccaaa cgctttttcg gctgtctgat gtgcattttg aacttggtct
     7981 tcaaatgtat attggctgag cgcttgttca atgatttcag gtttcaaacc tttttgctgc
     8041 atctgctgcg ctaatttctt tggtcctttg tcacttaagc gcatattcgt gcgcacataa
     8101 ctttccccat agaccaaatc atcgacaagg tccaattctt ttaagcgggc tacgatttta
     8161 tttcgatctt cttgaaggat ttccttttct tttaagtatg ttcggatttc tttttccgat
     8221 cgcaactgat aacttaagta attcattgct aggtgaaaac cgaaatcagc tccgctactc
     8281 ttcttgatct cttggatagt ttcttcgtct aattcccgtc cttttaataa tcgatagcgt
     8341 actaatacat cttcagatac ttgtaaagct tctccttcag ataaatctac gcgatagaaa
     8401 gggcctttgc ctttactgat tctcgtaacg gttaacataa acatcctcct cgaacatata
     8461 ttctctatca taccacagaa aatccccgta atcaggtatt atatgttaga ataaaccaca
     8521 gaaaaaagaa aaagggagat acttatggca gaaacaattg tcaaaactgg acaaacggtt
     8581 cctttgaaaa tcaagcgttt aggcataaat ggtgaaggaa tcggttattt caaacggttg
     8641 atcatatttg ttccatatgc actaccaaaa gaagaagtac tagtgaaaat cacgaaagca
     8701 actcctcgct atgcagaagg agaattaata aaagtcaaaa aaacaagcaa agatcgcgta
     8761 gtggcaccat gtcctgttta ctatgaatgc ggtggctgtc aattacaaca cttagcttat
     8821 caagctcaat tggattttaa aaaggatctg cttttgcaag cattggaaaa attcaaacca
     8881 gcaggctttc gatcttatca acttttgccg acgatcggga tggatgagcc ttggcattat
     8941 agaaacaaag cacaatttca gttacgaaaa aataagcaga ctcaaaaaat tgaagctggt
     9001 ttatacgaag caaactctca tcagttagtc cctataacag actgcttggt tcaagaacct
     9061 cgaacacaaa aagtaatgaa tactgttgtt caactattga ataaatttga tgcacccatc
     9121 tatgatgaac ggacaaacag tggaatcttc cgcacaatca tggttagaat cggtgttaaa
     9181 actggggagt tacaagttgt ttttattact cgcagtaaaa aattcccgca aaaaaacgca
     9241 atgatccgtg agatcaatga acgactgccc gaagttgttt caatcatgca aaatgtccag
     9301 gcacaaaaaa cgtctctcgt gatgggggat gaaacgatcc acttatgggg aaaagaagcc
     9361 attgaagaac aaatcaatga agtgactttc gatctttctc ctcgtgcctt ctttcaactc
     9421 aatcctcagc agaccgaatt gctttatggc gaaggaatca aagcattaga tgtccagcct
     9481 aatgaaacga tcgtggatgc ttattgcgga gttgggacga tcggcttaag tgtagcaaaa
     9541 caagccaaag aagtacgggg aatggatacc gttcctcaag caatcgctga tgcaaaaaaa
     9601 aatgcggaaa gattaggata tcacaacact cgatatgaag tcgggacagc agagcaagtt
     9661 ctgcctaaat ggttgaatga aggttttcag cctgatggta tcatcgtaga tccaccacgt
     9721 acgggtttgg ataacgcatt gatccaatca ttattgaaat accctccaaa aaaacttgtt
     9781 tatatctcct gtaatgtttc tacacttgcc agagatctgg ttaatttaag taaaaagttc
     9841 aagattgaat atttacagtc cgtcgacatg tttcctcaga ctgcacgttg tgaagttgtc
     9901 gtgaagatga cgagactcgc tgactaaatg aaagaggttg tgaaaaagtc tgccctgtat
     9961 caagtaataa gaacagccaa acaaaaatag ctcttcatgt tttatgaata ctgtacttgt
    10021 gaccgaagag ttaacaggag gacactattt atttaaaaaa agggttgtga catagttttt
    10081 gtcacagccc tttttgatta ttattggata ttcacgtcta tcacttgata aaatgcattt
    10141 gtcgtatcgc tgatattcca tactgctaaa atcacattat atccttttcg atcctgcgga
    10201 agtgtgatcg tttggttgac tgtttttggt ggaacagttg ctttgtcgtc tatttttgtg
    10261 atcaattcaa aatcactaaa tttcaaaggt tgattaggat tccaagttgg tttggtaatg
    10321 tagtaatccc aagtacttgt ccgatgttgt gctgttaaat tccaagtaat atttaatgtt
    10381 cctggattaa ccatgttttt atgccatctg ttagatgtct gttcatctaa aggctcaaag
    10441 ccgctgactc ctgcactagc aatttttcca tcaatgaatg tatttttggt agcttcgata
    10501 ctttgcggtt cccattgtgc tcttccgaca ttttgattta agtttcctgc tgctgatgtt
    10561 cctaggtatg cacggcttcc tggagaagta atataaccat gcgcaaaacc tggaatgcta
    10621 aaaccgaaca accctaaact aactaaccca ataaacaata acgtactaat ctttttcatg
    10681 tagaaacctc cttcatttga taaccacatt gtaaacgctt tattgtatta acacaaacat
    10741 tatgctttta taattttatg ttgaagtaaa aaaggtgaaa aaagtaagat gcagattata
    10801 acagaagtag acgagaaaag tacaaataga aaacatgaga atcaaatcat atgatctttt
    10861 tcaaaaacat cattcatata aacaaatgtt caacttttta ataacaacaa ttatcaattt
    10921 ttttcttact tcacaaacta aatacaggtc ttgctgtgag ccttcatggt aaactaatat
    10981 tatagaaagg agttagtaaa atgaagatga taaaagatat tcgtttttgg gtttgtgtta
    11041 taggaattgt catattaggc tttttatcag ggttgcttag cggaaatccc ggaaaatatt
    11101 actattcttt gcaacttcca ccatttgcac cacctagctg gatatttggg ccgatgtgga
    11161 ccttactgta tatattgatg ggtatttctc tttatttgtt acttaatcac aacaacaaaa
    11221 aacagcgaaa caatcttgtc ggtctttttg tgattcagtt catttttaat tttatatggt
    11281 ctgctctatt ttttaattta agaaatattt tcattgcagc aatcgatatt acacttttag
    11341 ttattttctt gtcagtgctt atgtatcagt tgtggctgca tcatcgcctg gcaatgtggc
    11401 tcatgattcc ttattattta tgggtactat tcgcaactct attgaattat tcaatttatt
    11461 ttttaaacta agcgctaaaa agagacgtcc tgtaatttaa cacatagaaa aagtgccacc
    11521 taagttagat tttttgtcta atttaggtgg cactctttat taaccaaact atttttttct
    11581 agtacccact tgttgttcca tactcattat tctggtcata accataacca ttttgttcct
    11641 gtccagcgta aggatcataa ctttgagtat cttgacttgg tgtatagtac tgacttgtat
    11701 cttctgtttg ctgataatag gtattcggat cttggtactg atcatatgtg ttttctgaat
    11761 aggaagaata atcggtgtac ccttcactat tcgatccatc accgtttgtc gaatcgccat
    11821 cttccgaatc atcaaagaac atcattgaag cattctcatt tttcaaatta ggtaatgtat
    11881 cgcttgtttt tatatttaat tgtttcttca gttcattttg gatcgaaaga agatcatttt
    11941 ttcctaaaat ttgatagtag atatcattga tcatttgtcc ttcgccttgc aactggtctt
    12001 gtttgattgt ttcaaacgcc ggggtgtagt tcgtcgctac atcaagcatg tcatcccaat
    12061 ctaagtccgt cgtcacattg ttctcaactg cttttaaaat ttttctataa ttgcttacgc
    12121 catctaaact taaaacttta ttgacgattt tcgtcactac ttcccgctga cgacgttgtc
    12181 gaccgatatc gccttctgga tcttcgtgtc gcattcgaga ataggctaaa cctttttctc
    12241 cattcaatgt ttgttcacct actggtacat ggacaccatc caaagtaaag tcaattttat
    12301 tattcacttt gatgccaccg accgcattga tcaaatcttc catgccatcc aaattgatcg
    12361 ttacataatg atcaatagga atatcgagca gttgctcaat tgaatccatg gccatttcga
    12421 caccaccaaa tgcgtaggca tgatttaatt tatctaccgt tccataacct acgatattcg
    12481 tataaatatc tcggtccaaa ctgatgatcg ttgatttttt ttgtttcgga ttaactgtta
    12541 ctaccatcat cgtatcagaa cgtccttgtt ccgttcttcc taatccaccg gtatctaaac
    12601 ctaatagcaa aaccgaaaaa ggttctttgt catcaatgct tactctctca gaacgtttgg
    12661 aggatattcg attagagttt ttagaaattt tgtttactgt ttggctcgct tctcccataa
    12721 atctgattcc gtatgctgat aatccggcaa tcagcaaaac gaatacccct aacaaggtta
    12781 atagaacttt ttgccatcgc ttcataatac acctctgttt cacattttct aaaatattgt
    12841 ctcatataac tgttcaatca gcaaattcac tttttaatca taagaaaact ttaaaaaaca
    12901 tttttgtgta acgattatta ttttttaaaa tttctcaaaa ttcttaacta tcataccata
    12961 ccgtgaaaaa gatcgaaaga ccgaatacac gcagttcgca atcgttttat ctttttttat
    13021 gtattttttc cagaaaaagc gaacaacaag gtattttcca aataatcgtg cagtaaaatg
    13081 atcgcacata cctttcccat ctttttaaca gtctttgcct accgttcaga ttatcattcg
    13141 atttcacgac aatttcttcc tatgactggt ccttttgtta aacccatctt ctattcgttt
    13201 tatttccagc tcactgcatg gctcttaaaa tgactcgaac tagtgattgc attaccatca
    13261 tcttttaatg gatagatatg ctcctcttca aagtaaaacg attttctttt aaagtaatag
    13321 aattgacatg gtaatgagta tgtatactag tggtttcaca aatggtcttt tattcaacat
    13381 ccaaaaactg aaatttcttt aaaatccatt actaacgaag aaaatgttcc ttgcttgata
    13441 attagttagg acatgtcaag tgaacaaatg catttactta gccaaaaaat ctaaaaactt
    13501 cataaataga caaaaagaca cgactctcta agaagtcgtg tctttattca acaaattatt
    13561 ataatttagt cacttcagca gcttgaggtc cgcgtgcacc ttcaacaatc gtgaattcta
    13621 cttcttgacc ttcttctagg ctcttgaagc cgtctccttg aattgctgag aaatgaacaa
    13681 atacgtcatc tccgccgtct actgtaataa acccaaatcc tttttcattg ttgaaccatt
    13741 taaccgttcc gtgttccata aatgtatatc ctccaaaaaa gttcttgcaa gtaaccttgc
    13801 aagaacatta tacccaattt agaaaacgct agcaagcttt cgctctttta ttttccaaat
    13861 tatttttaag actgatatga cggaggaaat ggttattttg ctgcttcgaa acgtttgttt
    13921 acttcatccc aattaataac attccagaat gctgcaatat aatctggtcg gacattttta
    13981 tatttcaaat aataagcatg ttcccaaacg tccaaaccta gaattggtgt ctttccttcc
    14041 atcaatggag aatcttgatt tgcagtagag gtaatagcta attttccatt ttccattaca
    14101 agccaagccc atccagaacc aaatcgtcca gcggctgctt ttttgaattc ttctttaaaa
    14161 gaagaaaaat caccaaaagc ttcattaatc gcttctttta tttctcctgt aggttcgcca
    14221 ccagcatttg gtgccatgat ttcccagaaa aatgaatggt tagcatgtcc gccaccattg
    14281 ttacgtacag ctgtcttgat atctgttgga atagcgtcca tatcagataa taattcttct
    14341 attgtttttt cgcctaattc tgggtatttc tcaattgctg aatttaaatt cgtcacataa
    14401 gtattgtgat gcttatcatg atgcagatgc atcgtttctt cgtcaatata aggttccaat
    14461 gcgtcatatg cataaggtag atctggtaat gtgtaagtca taaaaaaatt cctcctcaat
    14521 gttttcttaa taatgtctac attacaaaga gtaacaaaga gaaggaaatt cttcaattgt
    14581 tatgcctagt cgtctttttc actttgacct acttcttctt caaaaacctc agtttcttta
    14641 aaaataaaca acttgctgaa aatatagttc gcaaggataa caataatttg agtgatgatt
    14701 ttcgccacat actcattgct gttcataaca ttgatcaaca ataccattgc ccccatgtcc
    14761 aatccgtaag aaaggatacg gtagaagaag aacttaccgc attcaataag tgcatgtgta
    14821 aaattcggtg attttgaatg gaatacccaa agtttgttcg ttacaaaagc aaacaataca
    14881 gaacaaaacc acgaaataga attggataag gccatactta aatcaaaata ttggcgtgcg
    14941 agagcaaaac taacaaaatt tacaatcgta gccaaaccac caaaaaagag atagactagt
    15001 atctcccaat atccttttgc ttctaaatat ccttttaact gtcgatactg cttcatgagc
    15061 caacctacct tattaaaaat tcttatttat catactctat atttcaataa ctttcatctt
    15121 tatttaaaaa aattaatttt ttttgatcct ttgcctttgt ctctgcttgg atcgtcacat
    15181 gtgtaatacc atattttaat tttaataaag aaattatacg aggatatatc ttttcaatct
    15241 cgcttagtag ctgatcttca aaatcaatat gtccgtcgaa aataattatc ttttcgctat
    15301 actgccaaat atggacatga tgaacatcct ttaccccatc gattgcttca atttcttgtt
    15361 ccattttttc atagtcgata gcaggtgcac tttgtatcaa aatagcggca gcttttttga
    15421 tcaccattat cgcttctatc aatatatata tcgaaatgat cagtgtaata actggatcga
    15481 tccagtagat atcgaaaaaa cggatcaaaa ttgctccgat aatcacactt accgaagaca
    15541 gtgtatcact taacaaatgc aaataactag attttatatt caaactttca tctgcttctt
    15601 gtcgtaataa taatactgag aacaaattcg cgagcaatcc aataactgct actgttagca
    15661 tcaaatgact atttatcttt tctggtgaag tgaaccgctt gaatgcttca acggttaaaa
    15721 acaaggataa tatgatcaat atgaatgagt tgatgaaagc actcaatatt tctaaacgct
    15781 ggtagccata cgtacgtttt ttatttgctt cttttcgggc cattttttgt gccccgtaag
    15841 aaaaaataat tgccaatgta tcactaaggt tatggaaagc atctgaaaca agagacaaac
    15901 tccctgacat aatccctcca ataaattctg ataaagtgat caccgcattc aaaaccacta
    15961 ctccaaatag tttttttgat gatctctgtg gctgtgtagg cactattcct tcctccattt
    16021 tatttatatt ggatagtctt tagacaagaa taacattttt ttgatatttc accaatcatt
    16081 cagctgtttc cttaaagcac ttgtatttca cttgatactt cgctagaatt gcaagtgatg
    16141 acactactag atgaggagta cttatgacaa caaaacaatt aattatcagt tcctttatac
    16201 gattcaaaga cctaaaggag gcgagaaatg tcccattttg gaaagccatt gtctatcttt
    16261 tagttttgag tataattatg gctttgccaa tctcttttca agtttttcaa gtcttaaaca
    16321 acattcaatt ggatgggcaa aagattgcta ctcgcattcc tgattttaca atccaaaacg
    16381 ggcagcttca aaccgaagaa aaagaaggat tcatttacca aacaaactcc attatcttca
    16441 catttgatcc agagggaaaa cgaacagaac aggacatctc tactgatttg atggggaatt
    16501 ttgttagtgt cgggatgtta aaaaacaaac ttatcatagc tcttcctaat acaggcacga
    16561 cttctgcttt gttgaacaat aaccaattag aacttcccta caccaatgaa tctttaaaaa
    16621 acttaacagg taagcagctc cgttcttttt taagtgaagc atctatacca ctttggataa
    16681 aagtcctgac atttatattt tctatttatc ctagttttct gaatctgctg atcactttgc
    16741 tatttaccaa cattgctgct tttctctatg cacgctttcg actaacaaaa gctacttttc
    16801 ttgattgttt aaaaacattg atctattcaa tttcattgcc tactataatt gcaacaatct
    16861 tgatggtctt tcttccttct tttgacacta gcgccttcat tgcttttgca ggaatcttta
    16921 tttttgctca agctgtcaaa ggatgggcaa aaatatcgat caattaagaa ataaagacaa
    16981 ctcgttattt ctacttcaat agaccctttt agaggttgtg acattttttt gtcacagccc
    17041 cttttttatt tgaaaaatat ttctttctca aaaaacaagc aaggcgcttc aaagttcttc
    17101 ttgtatgcgc tacacttaga gtaaagaaag gcgggaaatt aactatgtca gaaagaatca
    17161 tttatgcaga tcgtccattt cgtgatgaat ttatcgaaga aatccatact atagcgcctg
    17221 gttatatctt taaaacagaa ttacaaccag aggatctaca aaatgtagag atcagtttgg
    17281 gctggaactc agactaccaa gaaaaactat tagcctcttc taccttgaaa tgggtccaat
    17341 ctatctcagc tggtgttgat cagctacccc ttgagacttt ttcaaagaaa catattttac
    17401 tttcaaatgg tagtggcatc cacagtgaat cgattacgga gcatgtctta ggaatcatcc
    17461 ttgggtattc tcgtggtctt tttcaagcac aaaaggcaca gagtgacatg aaatggttag
    17521 gttcatcggt ccattaccaa tctatccaag ggaaaaaaat actaatcatt ggaactggcc
    17581 atattggaaa aatgattgca aaaaaatgca aagcattaga catgaactgc tatggaatca
    17641 atacatccgg tcatccagca gaaggaatgg aaaagacttt ttctttaaat caactaaagg
    17701 aagttcttcc tgaagtagat ttcgtagtca atatcttacc tctaacggag gaaacaactg
    17761 ggctctttga tcagcatatg tttgaacact ttgatccaaa atctgtattc atcaatgttg
    17821 gccgtggtgc ttctgttaag acacaagatc tggtgcaggc tttaaataac cagcaacttt
    17881 cctttgcagc attagacgtt tttgaggaag agcctcttcc acaagatcat cctctttgga
    17941 aaatggacaa tgtactgatt acttcccaca ttgcaggttt gacacctgat tttcaaaaga
    18001 aattaatggc tattttttta accaatctga aaagctactt tgccacccat gagcttcaaa
    18061 ccaatcaagt aaaattgtct gcaggatatt aatagctgaa aaca
//
LOCUS       DAFGZE010000056        18016 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.058, whole genome shotgun sequence.
ACCESSION   DAFGZE010000056 DAFGZE010000000
VERSION     DAFGZE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.058"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(24..722)
                     /locus_tag="LTU54_002044"
     CDS             complement(24..722)
                     /locus_tag="LTU54_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HBL8296749.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="LTU54_002045"
     CDS             complement(715..2067)
                     /locus_tag="LTU54_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HBL8296750.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="LTU54_002046"
     CDS             complement(2290..3690)
                     /locus_tag="LTU54_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HBL8296751.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="LTU54_002047"
     CDS             3851..4588
                     /locus_tag="LTU54_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBL8296752.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="LTU54_002048"
     CDS             4713..5192
                     /locus_tag="LTU54_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HBL8296753.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="LTU54_002049"
     CDS             5189..5956
                     /locus_tag="LTU54_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296754.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="LTU54_002050"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="LTU54_002050"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL8296755.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="LTU54_002051"
     CDS             complement(6859..7461)
                     /locus_tag="LTU54_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBL8296756.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="LTU54_002052"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="LTU54_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="LTU54_002053"
     CDS             8142..8726
                     /locus_tag="LTU54_002053"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL8296757.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="LTU54_002054"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="LTU54_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBL8296758.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="LTU54_002055"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="LTU54_002055"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBL8296759.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="LTU54_002056"
     CDS             10627..10806
                     /locus_tag="LTU54_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296760.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="LTU54_002057"
     CDS             10828..11853
                     /locus_tag="LTU54_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBL8296761.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="LTU54_002058"
     CDS             12028..13272
                     /locus_tag="LTU54_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBL8296762.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="LTU54_002059"
     CDS             13425..14372
                     /locus_tag="LTU54_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296763.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="LTU54_002060"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="LTU54_002060"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBL8296764.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="LTU54_002061"
     CDS             complement(15274..15516)
                     /locus_tag="LTU54_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296765.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="LTU54_002062"
     tRNA            15637..15709
                     /locus_tag="LTU54_002062"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="LTU54_002063"
     CDS             complement(15828..16466)
                     /locus_tag="LTU54_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBL8296766.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="LTU54_002064"
     CDS             complement(16589..17221)
                     /locus_tag="LTU54_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBL8296767.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="LTU54_002065"
     CDS             17572..17871
                     /locus_tag="LTU54_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL8296768.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6169 a         3154 c         3557 g         5136 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta
    18001 ttttatcctt taatta
//
LOCUS       DAFGZE010000057        17737 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.059, whole genome shotgun sequence.
ACCESSION   DAFGZE010000057 DAFGZE010000000
VERSION     DAFGZE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.059"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..672
                     /locus_tag="LTU54_002066"
     CDS             <1..672
                     /locus_tag="LTU54_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296769.1"
                     /translation="SINDFKPKQQVLTEIEYYYRLRSLAVLGSNNEELQNHLNSLLDP
                     ILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDASSFRLADKDMEVLPW
                     IYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKFQVNLRKHYSGLLYP
                     NAVTMDQGFQLKDKELKNLYELAESKSDMYLDIIDNIINKSEDYLRKEINSEKELLNY
                     VHKIK"
     gene            complement(929..1264)
                     /locus_tag="LTU54_002067"
     CDS             complement(929..1264)
                     /locus_tag="LTU54_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296770.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(1439..1933)
                     /locus_tag="LTU54_002068"
     CDS             complement(1439..1933)
                     /locus_tag="LTU54_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296771.1"
                     /translation="MNKLPEKNRVFRLKGDLSQFKIDENGQFFVWMRHEHCWCNPFEL
                     KKENEIRTKIANGEWDYLFRWEDESFIANDMDHAFVSFLDNTWGTWYDCYLEKLGFQN
                     IDIDAIECLKEVDQKYEEEPYYLLQQKLANNSVFQVEIWENEDGTYEGLAQEIPFSDR
                     KSSI"
     gene            complement(1930..2157)
                     /locus_tag="LTU54_002069"
     CDS             complement(1930..2157)
                     /locus_tag="LTU54_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296772.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
     gene            complement(2324..4294)
                     /locus_tag="LTU54_002070"
     CDS             complement(2324..4294)
                     /locus_tag="LTU54_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296773.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRLEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAEINVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(4309..5175)
                     /locus_tag="LTU54_002071"
     CDS             complement(4309..5175)
                     /locus_tag="LTU54_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296774.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(5165..5398)
                     /locus_tag="LTU54_002072"
     CDS             complement(5165..5398)
                     /locus_tag="LTU54_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8296775.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(5410..7566)
                     /locus_tag="LTU54_002073"
     CDS             complement(5410..7566)
                     /locus_tag="LTU54_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296776.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAEDVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEDATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIIAVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(7586..7807)
                     /locus_tag="LTU54_002074"
     CDS             complement(7586..7807)
                     /locus_tag="LTU54_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296777.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(7917..8255)
                     /locus_tag="LTU54_002075"
     CDS             complement(7917..8255)
                     /locus_tag="LTU54_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296778.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNEQLG"
     gene            complement(8655..12296)
                     /locus_tag="LTU54_002076"
     CDS             complement(8655..12296)
                     /locus_tag="LTU54_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296779.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVVYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMIDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGQDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEDDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTALKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(12299..14248)
                     /locus_tag="LTU54_002077"
     CDS             complement(12299..14248)
                     /locus_tag="LTU54_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL8296780.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(14290..15348)
                     /locus_tag="LTU54_002078"
     CDS             complement(14290..15348)
                     /locus_tag="LTU54_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HBL8296781.1"
                     /translation="MKKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(15467..15922)
                     /locus_tag="LTU54_002079"
     CDS             complement(15467..15922)
                     /locus_tag="LTU54_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296782.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(16400..17203)
                     /locus_tag="LTU54_002080"
     CDS             complement(16400..17203)
                     /locus_tag="LTU54_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL8296783.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         5205 a         3526 c         2463 g         6543 t
ORIGIN      
        1 tcaataaatg attttaaacc gaaacaacaa gtactaacag aaatagaata ttactaccgt
       61 ttacgttcac ttgcagttct aggaagcaac aatgaagaat tacaaaatca cttaaatagt
      121 ctacttgatc ctatacttca gcaaaaaatg agagtttctt cgctaccatc ttcaacatta
      181 gaaacaatat ataatcaata tttatatttt ggtgaaaaag agatgttcat agattttatg
      241 atcgaacatc ttaccgatgc tagttcattc aggttagcag ataaggatat ggaagtatta
      301 ccatggattt acaataaatt aaatcaggag caatttaaca aattactata caacctaaat
      361 ggaaataatc aattttacaa aaacagtaat tttccgcctt ttatttctaa tctgttagac
      421 ttttataatc agaaattcca agtgaattta cgtaaacatt atagtgggct tctatatcca
      481 aatgcagtga ctatggatca gggttttcaa ttaaaggata aagaattaaa aaacctgtat
      541 gaattagctg aatcgaaaag tgatatgtat ctagacataa tagataatat tattaataag
      601 tctgaagact atttaagaaa agaaatcaat tccgagaaag aattgttaaa ttatgttcac
      661 aaaataaaat aatttcttat aacacaagat gcacttttat agcgtatatt tactttcaaa
      721 ttaaactaat tagttatatt ctcacaatag catttaataa taaatatact gtcgcttgat
      781 ttatatattt ccctcttctt tctttagaat ctctctttat tttgatataa taaaatagaa
      841 atcgcaccaa acaatgaaat ggcggaaaac atttcattga aaaaaggatt gaacataatg
      901 ttcaatcctt ttttatagtt ataatcaatt aacattcggt gtgtgaaatt gaatcttcga
      961 tgctttcaca tggaaaatta ttgattcctt cttcactcat ttcccatgta gtatagaaag
     1021 gagttaactg tcctccatac tcctgaataa taaaatattt tccatcattg atatggttac
     1081 aacagtacac tccaatagtt ggatactcga ctactaaaaa ttggtctgga tcaaactgtt
     1141 cttttttcaa catttctcgt atttgaaaca aatgatcctt ctctaagcaa tcaggtaatt
     1201 cctttattct ttgcgttact aatgacccct ctatcgcttt ccattcattt aaatattgtt
     1261 tcataacaat tcattccttt tatgttaact atttttccta ggaaattgtt ttaaaacgcc
     1321 actctcccag aaaacgtaag atttggttag ctagaatata ccttccagct aaccacaatt
     1381 ttccattact atattgtaag cttgctttca cttcataaaa attgctgata tactcttttc
     1441 aaatcgaact cttcctatct gagaagggta tttcttgcgc tagcccctca taagttccat
     1501 cttcattttc ccaaatttct acctgaaata ctgaattatt ggctaatttt tgttgcaaga
     1561 gataataggg ttcttcttca tacttttgat cgacttcttt aagacattca atagcatcaa
     1621 tatcaatatt ttgaaagccc aatttttcta gataacaatc gtaccaagta ccccatgtat
     1681 tatccagaaa tgagacaaat gcatgatcca tatcatttgc tataaaagat tcatcttccc
     1741 acctgaagag gtaatcccac tcaccgtttg caattttagt tctgatttca ttttcttttt
     1801 ttaactcaaa aggattacac caacaatgct catgtcgcat ccaaacaaaa aattgtccat
     1861 tctcatctat tttaaattgg ctgagatcac ctttcaatct aaatacacgg ttcttttctg
     1921 gtaacttatt catgcttcaa ccacctcata ctgttctctc atacctttga cttcattaaa
     1981 aaaaactctc gctttttctt catcagtaaa gtttttatac aactgattac aataatgagg
     2041 tttttgaagt ttctgaatac ctatactaac ttcaaatcct tcttcatgtt gaattaggat
     2101 tacttctgtg tctctaccta gaaatattcg atctaaaatt tttatgtttg gtttcattat
     2161 ttaacctccg ctataattat ttcactcagt aaggtttcgc tttatgaaat ttctatagtt
     2221 ctcttttcac aaaacgtacc atgttctcta agactgtttt tagtctcaaa tatacatgtt
     2281 ttttcttatc ctttccttat acactcaaaa tcaaagataa aacttaaaga ataaactctc
     2341 caataacaaa attattagtc agatccactg gtctcctgat ctgaaattta ttacttcttc
     2401 ctgttgtcaa atattgtctc aaatcaacag aaagtttttg atcatcttct ccttttctag
     2461 caggagcaac ttttttttca tacttcatta actctccact ggtcatcttt ccaatgatag
     2521 tgtaattaaa atcactttca aaactttggt aaatttttcc atctgtattg aatatatcag
     2581 ctgtttcaat atcttctttg tattcattgt cttctcctga accgattgta tcaaatagaa
     2641 aggcaagacg aattccatct tcttcaagca ttgttaatct ttttttaatt agcccaacgg
     2701 tttcaataga taacttatcg aagccgtgaa tcataacaat atcatctttt tttgcttgtg
     2761 atgtgatgaa atcaaaggta tttaaaaatt gagcttccat aacattgatt tctgctctta
     2821 gatcacctaa gttatagtaa aactgtaatt tgtcactttt accgatttct tttagtgtcg
     2881 ttctttgatt aaacatccta gagaagctgt ctaatgctgt tctcaatgtg tcttttaata
     2941 caataatacg atcgacttct ttttcagtac cagatttttt ggcttttgtt tcagcattag
     3001 tcaaatagct aataaaatcc ccaagaactg gcattgtatc actgctgttc accaaaagtt
     3061 ttgaacgttc ttcataatta atatcccaaa gtttctgact tatataaaag ttacctaatt
     3121 cttttttcaa taaaactcta gttgcagaat ctaatgttct gcctgctaac aaataaaaaa
     3181 tctgagttag tttatcgaga ttccgtctat ataaatcacc aatgtccttt tgattaccaa
     3241 aaagctccaa agagttaaga ccttcttcag ccaaattaac cttttctatt acactgttta
     3301 acgaaggttt ggcaacaaat gttctaccag ttggatcacc aaagtaatca aatttattta
     3361 agacaatatg aaaagttcgt ttatgatttg ccattgcatt attagcaatc aattgccccc
     3421 aaagtgatga tgctgagatt ttttcatcat agcctttaat tgtagcgttt ttgtgtgatg
     3481 ccactaaaac tttacttgta aaagaatcat ccagtgccat aaaagctaca ccacttgcgt
     3541 agctagtaga catagtgccg atagcccatc cattcgcatc atctaaacct ttacgaattg
     3601 catgatcatt tcctgcaaaa tccgaactca tagacgtata ctcatagaca ttcccgattg
     3661 gttcacttaa aagtctatca aacagatcac gttgatctcc tccatatgac ataagcttta
     3721 atccatacat tttttcatca tacaaccgtt ggagtttaat tagctgttcg ctgattttat
     3781 taggagaatc ggatactaat aggatttcta gcttccaatc aataatttta tttgccatgc
     3841 tgtctagtaa agatgctgta ttcaaatcca taatccgcat aacagttttg ctaatttcag
     3901 catctgaatt ttttccttct aaattatttt cataactatt cgttgatccc ccaacattct
     3961 cgtgcattat ttcttgttga tctttttgtg acactggctt atctgcttca attagatagg
     4021 cttttacccc ttctagtctc acttcaggaa tagctttaac catccaacca aatccataat
     4081 ttctattcat tccataagta ttcactaatt tcacaattgt tccccaccgc ttacctgtcc
     4141 taatgtaaga tggtgtaaat tgataaccaa ctaaagatgt taaatcacta acaaaggtat
     4201 tattcactac ctttgaattt ctagtttttt gttctttttt agtttttctc ttttttttga
     4261 aatttaatag ttgtgatcta cccataattt ccatttaatc tctcacctct atttcagtgc
     4321 ataaatatca gataatattt cttttgtttg tttagctgac aactgagtga cactataata
     4381 aagtcctcta ctcgtatagt tttctaaacg ttccacacct tcatccaata ccatcttaga
     4441 ttgagatttc aagataagat attggattac tgttgttttc acaccatcaa tatattctga
     4501 tacaaatcgt tcttgctgat ctattatgtc aaagactgct ttgttactcc ttgcatttcg
     4561 tttttgtatt ttcatatttc gagtttgtcg ttccgtattc tgaggttgag atgaagtgat
     4621 tttgatttca gctaaggaac gatctctggt attttgatat tgtattgatt tcgattcctg
     4681 agccataact tcttctggaa aagcggttag tgatgtagcc ccatctagat tgaacattaa
     4741 tgctacactc ccatctcgat atacgattgt tccatcttgt ttcactttta aaatctctgt
     4801 gcctcgaaat ggtttctttt tacccataga tactcttgct gaattaacta ctgatttgaa
     4861 cagtctaata gaaccagaaa tcaatgaacc actagatgga tcaataaaca tatactggct
     4921 aattagaata agaaatccaa ttatcattaa taacgttgga acaaaaataa atttcagttt
     4981 aatcgtatca gaaactaagt aaacaattga tccaagaaca acgactgcaa caactatagc
     5041 tgatacaatt aatctttttg ctgtaacaga ataggtgcta ttgacatgct tttcaagcca
     5101 ttcaactctg acaattgttt cagaaggttt ttgatgtaag ttatgtttaa atccatagtt
     5161 tgagttatct gccatcagaa atacttcctt tcacgttcaa cttacggtaa acacctacag
     5221 ttatcactat tgcaacaata agcaagccaa ttaaagatat tgacttgctc attgtctcgt
     5281 tcgtttgtgg tagttcattt acctgataca cgagctgtgc ttcctgtaag tgctgagaag
     5341 aggtcatgtt ttcttgattg ttttgaatta ctggataaac tattttttct tctcgcataa
     5401 tagccctcct tagttattat cagataagtg agcttgaatt ttgtactgat ttccgtctgg
     5461 agttttcaca actgcttttt ctccatcaat agaaatcact tcagccttag ttgttttccc
     5521 atcaatactg atgacaacat ctttcccaac tttgaactct gtagttggtg ttgcttcata
     5581 gctactattt gttgaagcag agttcgtatt tgtcccaatc gaattttcca ctctcgaaat
     5641 agatgtttga gtttgagcct gtgcttgttg attattatga ctaataaagt aggacgatcc
     5701 caagatcgtt ccgcaagcca ttgcagcgac agcaatgata gtagccatct ttttacttgt
     5761 aaaatcacgt tttgaacttg attgattttc tgtagcaact gctaatgatt tcgttttttc
     5821 tgccatttga gctagatcac ttcgggaacg agataagtct tctcttaaag cttcactttg
     5881 ttctgccaac ttttcgctat ttaatttata accctctgct aaatctttga cctctttcat
     5941 tcgatcttta tattcttcga tagttttttg ttgctctaca agttttgttt ctaattcttt
     6001 cgtgttgtta gctacctgct tttcaacttc ttctttgttc gcaaaatatt tgacaaccac
     6061 atctgttgtt ttctcttttg tatcatggaa ctcagcttca ttttctgact ttgttttttc
     6121 gacaatctct aaagtttttt cttgcacgtc tgttaatgat ttttcaattt ctggtttcaa
     6181 tgaattgata attcgttcca ccacaacttc ttttgcttct tggctttgtt tcaatgcatc
     6241 gtttttaata gattcaattg cttcttggta ctgttgatca agactttctt cagttgcttt
     6301 tctatattca tcttcttgtt gtttaacttc tggtaaatgt tcagcatcat accgttgctc
     6361 tagtttcttt agttcagcat ccaaataagc ctgtttcgcc atatcataat ctttacgaat
     6421 gatattggat ttttccatca ctaaatcatc aattttagat gcttctgctt cgtagctctc
     6481 tttagcaacc gatagtgtat ttgtaaactg attttgacca tttaatgagg ttgcatcctc
     6541 tatccgcaaa agctcatcgg ctaacatttg ctttcctttg aggatcaatt gctccttcgt
     6601 cttatttaat tcactgttga ttttattatt agcaatagag atacgagcgt tcatactctc
     6661 tttagctaaa aacagcaatg gtttcaactc aggtatggat agcatctcct cgtttaaatc
     6721 gacagggtca ttaatctcat ctaaaactac aggttgaaat ttgtcagcaa tgatctgttc
     6781 aaaatttttt tctaaaaaat cagcatctaa gtttttcggt acatcacttt cagatgtgat
     6841 tttttcagct tcataaacat aattttcttt gtcactttgg gattcgggtt tttcatcaat
     6901 ttttggttgt tggtaggttt ctgtttgtat ttgtgtttct tctatgctta cttcttggtt
     6961 ttctgcttcg gttggtttgt tatcatccac aaagagagct tctttttcat ctggtacatc
     7021 ttctgctacg gcttcaaatt caacttcttc agaagagttg aaggtctcag caattcgttc
     7081 taatccatta gtagctactt ctaattgctc tactgcgcta tagaagtctg tagcttcttc
     7141 aacttctgat tcaaacacat taggatcatc cggaataact aataattgac cactactatc
     7201 cataaaacgg acacctgcta atagcttctc tttaaatttt tctggtagat cagctacata
     7261 attcaaactt gacttcaata gaacgacata cttttcagaa tcgagaatag catcatcctc
     7321 atctataact ccctcactgt tgagcttttg tacatcttca attaccatat aatttttctt
     7381 tggtaaatct ttggtcagca acgcatcgaa ttctttccat acctttgctt tagctttttt
     7441 tgccttcgct tctttttctt cttttttgta cacttcgagt aattgtggct tcaaagagag
     7501 tgcaactaca ggaactgttt ctgccacata attaatagat tcggtaatat ctttgaattt
     7561 tgccattatt gtaaaactcc ttttttcagt caatagttct tttctgtttg tctagcatat
     7621 atacatatgc tttttcgaat ttaataagcg tttcaagtct ggctttttca atctttttac
     7681 tcccatttcg ataatcatag aactggttaa cattcatatt cattatcttt gccagttcgg
     7741 tacctttcag actgctcatt aatacttcgt tggcgtgagc taacattttt tctctatcaa
     7801 aaaccatctt acatttactt tcttccctca cttctaaact acgcttggag cgtagttttt
     7861 tggtaaaaag taaattgaac aaatcggcat attatccaaa tttgttcaat tcacttttaa
     7921 cctaactgtt cattttttgg aaattttgaa actagataga taccaacaac tggaataagt
     7981 gctgatatga tattagcttt ttggttcaaa tcattacctg ttttagtacc cattgtgtaa
     8041 agcataacgg ttgtaattac aggaataata aagattgcaa taatcgcccc aacatatttt
     8101 aatccatctc cccattttgc atcaccaaaa aatttcattg cagtgaatcc tggtttcaac
     8161 gaaaaaacga atgccaagaa atacaaaaca acttgtacta aagttttcat atcaggcgtc
     8221 attccgctaa ccaaattttt aatagaatcg aacatataaa aacctccttt ttaatatttt
     8281 taataagtta attatatcat cgacaaatct tatttgttca cttgtcaatg atctaactaa
     8341 gaaaaagacg tctcgatatt tacatcttta tgttatcata ataacataaa ttttcgatga
     8401 ttaaaagaga ttttccatta aattatttca ctttttttat tggctaaaaa tgctggttta
     8461 tcaagttttt gtttagataa taattctgtt ctttaccagc ataagccctt tttaattata
     8521 caactttgaa cagtaaacat ttaattcatt tatagacaat ttaaaataat ttttttgcat
     8581 aaatagaaat tttccttgac atcttcagtt agaaatcaaa gtaaaaagaa ggagcaaatc
     8641 aatgctcctt ctttttactc gtcttcagac ttattttcag ttgatttttg attaccgtat
     8701 tttacaatat agtccttgac tgacatggta gatttcttca ctactttacc agttttggat
     8761 gctcctgtag cagtagcctt ggcaacctta gccgttcctt tacctatttt aatagcttcg
     8821 tttttagctt cataggttat ccctgctaga ctaccttttg gagcttttgc taaagcttta
     8881 tctgctgagg tgtaaacatt tttaccagtt tttgatagat attcaacggc tgatttagct
     8941 gttgatggtt tcttggttga ttgattagat ttccctgaat tatctgaagt agatttagaa
     9001 ttatttgttc tattattttt atttgtcagt tcaggttctc taattgattg attgtctttc
     9061 gacattcctg atccaatatt tgtttcatat ttatctgttt gattaacatt aggagtagga
     9121 atgaactttt tcttcacatc tgattgttct ttatccgttt cttttgttag cgattctctg
     9181 tctttaagtg ctgtgtcatt ccgaatagca ctattatcaa agttgctgtt aagctctctt
     9241 gactgacttt caccagatgc tccatctcct aattcccgat gcactctgtc tgacacgtcc
     9301 tcaccactca tttcattcct cagttcttga tgagtttgat ctaaagtaat ctcgtcatct
     9361 tcattctcag gaattgcaac agcaattggc gtatgccgtt gttgttcgag ttctctattt
     9421 cgatctgcca caagcgcatc tctcgtctca atgatattcc catcctcatc gactaaatct
     9481 ccattcacat tatatccatc gctgtgttta ccaagtttat taccaaactc atcaacaaga
     9541 tttccatctt catcaactaa acgacctttt tcgtcaacta aacgtccgtt actatcctga
     9601 ccaaaatcac catttagcaa cctgcgtcca ttttcgtcaa caagatttcc atcttcatcg
     9661 actaagcgac ctttttcatc aattaaacgt ccttcgctat ccataccaat attaccatct
     9721 tcactatctt ccaatggttt accattagta tctataaggt ttccatcttc atcgaccaaa
     9781 cgcccttttt cgtcaataaa gttgccttta gcatctttac cgtatttact atcttttaat
     9841 atttcaccat tttcatcgac aaagtttccg ttttcatcaa tcatacgtcc taaatgatca
     9901 atatattgtt gggagttatt acgtagttga tcttgacgaa ctaaatcttt gaaagaggta
     9961 ttattgattg ttgaaccaac tccgccaccg ttgaatgaca ttccttctgg tgtttcagga
    10021 attactttat cagcaatgga cttagcatca gcattgctat ctcccatatt gttcaaacgt
    10081 tcagccattt tttgcgctcc aactgctcca agacctcctg caaccatatc ttttactgag
    10141 ctgttgccac catcataata gggattttta gctccttggc ttttcttgat tgctttagaa
    10201 acgccgtcca caactcgcac agcatcattt ctattaccaa ataataactt agcacgttca
    10261 tcaaacccaa acgctacttc aagtggtaag ccaataagca tattaatcaa attatttact
    10321 ttttcgcctt ttttaaagac taaaagatat cctacaccta aaatcatagc agatcgaaca
    10381 atatttgcta cgccatcgcc aactaatgta ttaggaatga atcccacagc agtatcacca
    10441 aatgcacgaa taaaagtaat cagaaagata ggtaattgta gtccgatcag agatgttgta
    10501 acaacaccta atgaaattgc catcacacct gctcctgatg caagtgatcc tgttagaact
    10561 acttcccaaa tccgaataaa ttcacgttta aatgctttca cgatattcac attgaacatc
    10621 gcccaagcac atgctaagta aagaactgct aaaacgattg attcagcgtt catcgctagc
    10681 cacgagccaa acttacccat cgctccatga ccaactaagg atgctgtacg ccattctgtt
    10741 tttactgttg acatattctt agcactgcct tgaattcctg aaggagcgat attatacgca
    10801 taaaacttag ctcctgatgt atcaagtgct gattggagca ataaagctac cgactgtgtg
    10861 ctaaaagttg cattgttagc aacgccccaa ggttgagtag catcaaagtt atgcgctgtt
    10921 ccttttccat caggcttgcg attgtcgtct gtcacatttt gatttgctga ccaaatatat
    10981 ccatttaaat tggttggttt cttaattgca cttccttctt ttccatcccc taaattatag
    11041 cctgtccaaa cagcaggcgc tcttcccata taagcatcat tttcggcgac attaacatca
    11101 ccggcataag tattaatatt gaaagtatca ttcgatgacc attcttttaa tagagccata
    11161 ccgactgaat tagaatctag atcattattt tgtctggcat aactgctagt attgatcgct
    11221 cttatcattg cagatttttt agatggttca taatctgtat caacgaactt gatttcggat
    11281 gtaggctttc cgtcaggtac atcaccacca gtatcagatg atggagcaag attttgagta
    11341 gaagcccaaa gtcttgtgtt aatgatgtag tcagaaacaa catttgttgg tatatctcca
    11401 tctttttttg ctgactggat gccgttgata taaactccca atagcgatcc tgccactggt
    11461 aagaatataa acaatacaaa aatacgaaca atccattttt caagtactgg tgataatccc
    11521 ttcgagttaa caccatcttt cgacaataat ttcattcctt gataacctag tattgctgtg
    11581 aaaaagagca tagataagct tgctatcgct ttaaataatt cattatttaa tcccaaattg
    11641 ttaagcaagc tagttaatgc tttagatacc ggattagata catccgatcc accttctccc
    11701 caccccataa gagaaaatat attcatactc atgaaaaatt ttccaaggag ttcatttatg
    11761 gattcagcaa tattagaaag gattaaacta attgatccga taaaactacc aatcgtccta
    11821 ccaatacttc ctagaatgga tgatgcttga ctggcaatag catttcctga gactgtttca
    11881 atatatccgt agtaactata agttgctaaa gtagatgcta gttgagtacc tgcattaccc
    11941 cctgcaatcg aattaccttg tttagaaatg tcagaataag ggaaatcggc actgatacca
    12001 ccttgtccta acaaattcat ttgtccagtt ttagcggcac tatcattaac ttctttatct
    12061 cccattagga tacgagcata tagtgtccac attccttgtt ctgccggttt actttccgca
    12121 tatacaacgt tcttatttat aactgtttct tgtttgctat cttgattgtc aggagttgga
    12181 tcattcattc caaaaggagc gcctaaagta actgctaata ctcctccaat aactagacct
    12241 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
    12301 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
    12361 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
    12421 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
    12481 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
    12541 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
    12601 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
    12661 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
    12721 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
    12781 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgcta tattcattag
    12841 aattttcctt gtttgtaaca cgagatagtg agctatatct gtttaacgaa tcgaacatct
    12901 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
    12961 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
    13021 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
    13081 aaggatcatg ccttacatga gtacctatga gtcgtctaaa acgcattgca tcatcttttg
    13141 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
    13201 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
    13261 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
    13321 ttgttatagt ataaactgct aacggttgcc cttttatgga ctttaattcc tcttgaagtt
    13381 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
    13441 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacgg
    13501 caattgattt agccggttcg gacaaatcct cacgataaag cttgtttgct tgtacgattt
    13561 cttgaagtaa ttcatcagga atcctttgcg ataatttaaa agaagcctga ggatcatctt
    13621 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatca ctttttagtt
    13681 cattcaattc taacggttgt aaccgtctcc ctaaggcatc atctcttttg atatagtttt
    13741 catattcttt tcttgtagta gcggtaatca attgaagtga ttcatttgct aacggttctt
    13801 ttagcgaatc acccccaatt ttattgccac taccaaaaga gctaacaact ttatgaaact
    13861 cgtcaatgaa gaggacaaat tcattttctt tgtcctcaga ttttaaaatt cgctcatatt
    13921 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
    13981 gcaattgtcc gattgataat ttgtatactt gtactttctt tccatcttgt cgctgtcttt
    14041 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
    14101 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
    14161 ctaacttatg tttcggtggt ttcattggtg tgagagcatg attaaattca gtttgaatat
    14221 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
    14281 aatttacttc tagtcagtca gagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
    14341 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
    14401 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
    14461 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtagcttcaa ttttcttgat
    14521 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
    14581 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
    14641 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
    14701 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
    14761 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcatc
    14821 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
    14881 ttcatctaat ttacctttta ggaataagaa agcatcccca tcagtactta acattccata
    14941 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttgtagtt
    15001 ttttccttgc gtagataaca actttctagc gttttgcgag taaccgagtt ttactactgt
    15061 tacatctctg ttttgatctg tccacacatc tgaaatagtg atctctgctc ctgatcgagc
    15121 aaactccaat gaagagccta taggtgttgt attgttcacg agataataat tatagtttcc
    15181 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
    15241 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
    15301 aatactgtat tgatctttgg tagatgttga attgacgttt tttttcatta gtttgtccgc
    15361 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
    15421 ttaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
    15481 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
    15541 gatagtaatt cccctcggct ttgttttttc ggattttgct aaccattgga gtaggtacaa
    15601 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
    15661 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
    15721 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
    15781 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
    15841 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
    15901 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
    15961 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
    16021 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
    16081 tcatgaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
    16141 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
    16201 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
    16261 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaattgtt
    16321 tttttcttga cttgtccaaa agtgttaccc atactttttt aaagaagcag gttgtattaa
    16381 aattacaacc tgcttcttct caatcttctt ctaaccaatt atgcattgaa atggttggca
    16441 agtttgattc tggctcacta aaagtgaatc tgagccgatc aactcttctt cctttctttg
    16501 catagatttt ttcataatca aaagtttcaa atacctgtat tccgttttca tcaggagcta
    16561 aaaattcttc tttcgcctgc tctagtattc ttttgtcaat atttgtcatt ctataacttg
    16621 aaggtatact taatgctctt ctaaaatctt caactttgac tacccaataa ccatttcgcc
    16681 actctttatt tctgtattgc atgagatgac gatacaattc ttttgtgtag ctacttctta
    16741 gttttgtaaa attatcaagt tcaaaacgag taaaattttt gccaattgag tttaagataa
    16801 attcaaattc ctcatttgcc ttaattgtaa ctgtttcctt ctgtttatct atccggaatt
    16861 tttgaaaaag ccacagctgt tcatagacgt ctgaatcttc aaaacggtag gttaaagtac
    16921 ctagtttgtc actcaaagct tttaatacag aataaaaact gtccttttct cttctgtcgt
    16981 atttgaccaa ataacgaaag aggttgaaat caaaagttac ttctgctgtt ccttgacgtt
    17041 tcatttttga taaaatagcc caaaacacat ccatttcaac aggcttaaat cctcgcaaag
    17101 ctgttgtatt cagaccattg ttataacgca caattgcatt tgtttgttct gatactaaat
    17161 ctacatcact atcaataatc ttttcggtaa cttcttgaga cattgattac tctccaatct
    17221 tctttttgcg ctcctttaat catgttaatc tggtactaat taaaggaaac atttttttct
    17281 gcttgttacc taaattaaat cacaactaaa ggtaacagtc aacaaagatt tgttactaat
    17341 ctaattttct attttttcgc cttccaaatc aagaaaaccg aaggtaacgt cttatcctag
    17401 tatgatacct caaatgtcca cttttgttac ctttgctgtc caaaagtgtt acccttactg
    17461 tccacttttg ttacctttac tgtccaaaag tgttaccttt ataccccttt aatacttact
    17521 ctcccaagag attaagcctc ccctaacaga tatattaaca gatatttaac agatatatta
    17581 acagatattt aataaaggag gattactcct ctcttatttc aaaatcttaa aaaaaaacta
    17641 acactatcta ggagtttatt ttgcttgtgt aactttgaat aattgaaaaa aaggaaaagg
    17701 aggaagcttc agcttcccct tttatttata tttataa
//
LOCUS       DAFGZE010000058        17557 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.060, whole genome shotgun sequence.
ACCESSION   DAFGZE010000058 DAFGZE010000000
VERSION     DAFGZE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17557
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.060"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            283..522
                     /locus_tag="LTU54_002081"
     CDS             283..522
                     /locus_tag="LTU54_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296784.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="LTU54_002082"
     CDS             complement(884..1144)
                     /locus_tag="LTU54_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296785.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="LTU54_002083"
     CDS             complement(1329..1826)
                     /locus_tag="LTU54_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBL8296786.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="LTU54_002084"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="LTU54_002084"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HBL8296787.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="LTU54_002085"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="LTU54_002085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HBL8296788.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5210)
                     /locus_tag="LTU54_002086"
     CDS             complement(4548..5210)
                     /locus_tag="LTU54_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HBL8296789.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(5220..6035)
                     /locus_tag="LTU54_002087"
     CDS             complement(5220..6035)
                     /locus_tag="LTU54_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296790.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(6297..6740)
                     /locus_tag="LTU54_002088"
     CDS             complement(6297..6740)
                     /locus_tag="LTU54_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HBL8296791.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(6874..7212)
                     /locus_tag="LTU54_002089"
     CDS             complement(6874..7212)
                     /locus_tag="LTU54_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8296792.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(7200..7577)
                     /locus_tag="LTU54_002090"
     CDS             complement(7200..7577)
                     /locus_tag="LTU54_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL8296793.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            7801..8994
                     /locus_tag="LTU54_002091"
     CDS             7801..8994
                     /locus_tag="LTU54_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8296794.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(9160..9582)
                     /locus_tag="LTU54_002092"
     CDS             complement(9160..9582)
                     /locus_tag="LTU54_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296795.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            9977..10171
                     /locus_tag="LTU54_002093"
     CDS             9977..10171
                     /locus_tag="LTU54_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296796.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            10168..10662
                     /locus_tag="LTU54_002094"
     CDS             10168..10662
                     /locus_tag="LTU54_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296797.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(10824..11996)
                     /locus_tag="LTU54_002095"
     CDS             complement(10824..11996)
                     /locus_tag="LTU54_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8296798.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(12202..13017)
                     /locus_tag="LTU54_002096"
     CDS             complement(12202..13017)
                     /locus_tag="LTU54_002096"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HBL8296799.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(13167..13613)
                     /locus_tag="LTU54_002097"
     CDS             complement(13167..13613)
                     /locus_tag="LTU54_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL8296800.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(13610..14614)
                     /locus_tag="LTU54_002098"
     CDS             complement(13610..14614)
                     /locus_tag="LTU54_002098"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL8296801.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(14654..14983)
                     /locus_tag="LTU54_002099"
     CDS             complement(14654..14983)
                     /locus_tag="LTU54_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HBL8296802.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(15109..17436)
                     /locus_tag="LTU54_002100"
     CDS             complement(15109..17436)
                     /locus_tag="LTU54_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8296803.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         5070 a         3484 c         3054 g         5949 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaacctgg accaataaaa
     5041 agtaaagaaa aaataaagga aaagacattc cttccatcta tcgacaaaag gaacgcagct
     5101 gctatataca agatattgac acaccaaaaa caaaggctac ttgccaatga ttgatacaac
     5161 caataaaaga attccatata aacatttttg tcaaaacgct gctctttcat aatcacctcc
     5221 tattccttca ttcctgatga agcgatccct tctacaaaat atttttgcat cgctaaaaat
     5281 ataaaagcaa caggaagaac agaaacaact gaggcagcca tgattaatgc gtactctgca
     5341 tcaaatagcc caataaacat tttcaaccct aactggattg ttttattttc cgtagaagac
     5401 aaataaatga atggccccat ataatcgttc catgtattaa cgaatgtgat gatcgtcaaa
     5461 ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc gatagattcc ccactctgat
     5521 aagccatcga tccgcgcact ctcacataaa gaatctggga tactactgta gtattgcttc
     5581 aacaagaaca caccaaaagc attgaacgct tgtaatagca ccaacgacca caacgtatat
     5641 gttaaaccca cttttctcat cataatgaac tgcgggatca tataagactg ccaagggaca
     5701 gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc ctttaaaatt tagtttagaa
     5761 aacccgtaag cagcaaaact agaagtaaac aactgaatca acgtgatcac aacgcttaaa
     5821 aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag accaaataac cagataattc
     5881 tctaaatgcc aagttttagg gatccattga atcggaatcg tgaacacttc attatttgct
     5941 ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg tcaataaagc taatacaatc
     6001 agcagaatca tacccaacca ttgaataaag ggcatgtttt ttgaacgaat ttttgctttt
     6061 tttctttcat ttcgaacacc tccaaagtta taatcttcat ttctttgtaa tgattacaat
     6121 ccagaacaac ggttattata gctgattcat ttccgttctt cttatgtttg taagcgctta
     6181 tttaacaagt tatgtaaatc tctttatttt ttttgattta aaaatgaaaa aatacaaaag
     6241 caagactgtc atacgtctcg cttttgtatt gaaatttctt cgattagaga tacttattac
     6301 tgttcaaaag cttctttgaa acggtgaatc aattcattga ctacaggtga taaaatccga
     6361 ttctttttcc agactagcat gcagttcgtt tcaatcattg ggtcaagcgg aacgaatgtc
     6421 actcgctttg aaagatgact tacagcacct tctatgacta aagcagcacc tacctgattc
     6481 tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc ctgtgatcag caactgctcc
     6541 ttttttatgc ctaaccagtc agcaaacatt cgctgtattt ctggacgctt tgagatcaaa
     6601 agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt tttttgctaa aacagaattt
     6661 tctaaaatga gcaatcccca tttttccttt cttggtaaca aaatcgtttc gtatttctct
     6721 gtatctactg gatctagcaa aatcgctaaa tctaacaggc ttttttctag tcactccgtg
     6781 atatcatcac ttgttccgct gtggatatga aattgaacgc tgggataatc acggatcaac
     6841 ttttctagta atagtgccaa tgtattcgaa ttatcagctt ccacacagcc gatcgtaaat
     6901 cggccgctga ataattgttt ttttctgttc tcgaattctt gagtggtttg attcgttaaa
     6961 tctaaaattt cttctgctcg gcttttttag aaaataacct gcttctgtca agatcatttt
     7021 gcggttttcg cgtacaaaca gttctgttcc tagttcctct tctaactcct tcagttgtcg
     7081 gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct tgagagattg ttccttcttc
     7141 tgcaattgtc caaaaatatt ttagtaagcg taactccata gcgatgctcc ttgatttttt
     7201 tacttgatac attccatttt atttctagat aacacacgat catatttctt gatttttcct
     7261 tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt tcgtcaaaag ttcactttct
     7321 tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc cttcgctaga taggcgaata
     7381 tattcaatca aagactcgat cgataatccg gcatttcgca tacagatcgt aaagtcgacc
     7441 cattttaggt cttcttctgt ataatcacgg attccgcctt ttgtccgatt aactggtgga
     7501 atcaacccga ttcgttcata atagcgcaat gtatcggccg aaatgccctt ttcttcactg
     7561 acttgtttga tattcatctg atcactcttt ctcttaattt cttgccactt tacttatgta
     7621 ttttatttat tctacaactt taagtgtact ccaagtcaat actcatttct attaaaacta
     7681 agctggatta tctatatcgg ttgtttgtgt attatttcaa ataaaaaagt cttgtttttt
     7741 tattcttttt tggatatact tttaacaatt tagggaaatt ttaggaaaag gggtataaaa
     7801 atgacaacgt atcagcgaaa ggtattatta tcgacatcgg cgggaattgc tttagagaat
     7861 atggatatta tgtttttagc tttttcatta tcttcaatga ttacttcttt tcatattagt
     7921 ggtacgcaag cgggtttcat ttcaacgatc actaatttgg ggatgctcat tggaggtatt
     7981 ttttttggca tcatagctga taaatatgga cgtgtgaaag tattttctca aacggtcctt
     8041 ctgttttcga ttgcttcttt attgatgtat tttgcttcaa acatctactt ggtttatctc
     8101 tttcgcttta tcgcaggtgt aggagcggga ggagaatacg gggcgtgtat gtccttgatt
     8161 tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt cggttgctgg tattggtgcg
     8221 caggtcggtg cggctttggc tgcgatttta gctgctgtga tcattccaac gtttggttgg
     8281 aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg tcttgttcat tcgtcgcggc
     8341 ttgactgaac caaaggaatt caaatctgta caatctgccg aaaagaaaac caaacttagc
     8401 agactgtttg aatcaaaaca tcttgcttgg caaacatgtg gccttagtat catggtaact
     8461 gtccaaattg ccggctattt tgggttaatg aattggctgc cttcaattat gcaaaatcga
     8521 ttagggcttt cggtttctgg gtcttcattg tggatggtca gtactatttt aggcatgtct
     8581 ttggggatga tgacttttgg cataatcatg gataaattgg ggcctaaact agccttttct
     8641 ttgtttttaa tctgttcatc cttatctgtc tatctacttg tgttagccca tagccagtgg
     8701 agtttggtag cagccgctgt gatagtcggc tactttatca atggcatgta tggtggatat
     8761 ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg cgacggcgaa taatttcatt
     8821 atgaaccttg gacgagctgt tggtggtttt tcttctatcg tcatcggttt cttgatggat
     8881 cattatagtt taactgccgt aatcttgttt ttgagcggta tttacctagt cagtctgctg
     8941 gtcttgacaa ctcttactgg tgttacagca ttaagggaaa gcttgcagca ttgatagttg
     9001 agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc atctgcggta ataagaccag
     9061 ccaaatcaaa aacatgagac actgtttccg tttggctggt cgtattactt gatgcagggc
     9121 agctttttca actgctaatt tccttctatc tggcggcgac tattctttga cgtccgtcat
     9181 ttcaaagtac tttactaata acttagcata acgaaaaact gtggatacac tcgtatattg
     9241 agcgtctgca taatcttcca aatttttctc aggatgttga tacatgaatt caatcaaatg
     9301 ataattatat gattttctgt agaaatgaag ataaataatg tagattggga agttctcctg
     9361 cttttccata gaaaagcgcc gattattctc attcttcaaa atgaccttat caggatattt
     9421 ttggaacaat tcttctaaat ctgttagata attcaagata gacttacttg ataccttcaa
     9481 ttcgttttgt agttcttcta ttgaacacca acgttctgtt tcaagcagta aatgaatcat
     9541 tttaattttc ttttgagaaa cttttgttaa aaaagtctgc atattttctc ctcctgtatc
     9601 tactttacta tactacatac aagaaaaaaa acggatacaa atatcatatt gtcacaccgt
     9661 caatagtgca taaacctgtt attatttctc tttttttcct aagtattcta ttgatatgaa
     9721 tcatttaatc gtttattata taaatgtcaa taatctaata ataaaataac gtacatattt
     9781 tattattgca tacttaataa cagaactata gcaaaaaatc ccgtgtatca acacgggatt
     9841 agattaagtt tcctcaatca ctattcataa cacaattatt ataacaagta agtaaacaat
     9901 tgaaaaggac atttattgaa aacggttgtt ttttacttaa ggaggaaatt tagcgaaaaa
     9961 attagttgca ttgtttttgg tcgcaggaag tttgttgagt gggtgtggtg ctactacgtc
    10021 tactactcaa agcacaagtt cagatcaggc ttccagcagc agtaccacga tctccgaaag
    10081 tacggcagat tcatcgaaag aacaagaaca aattgaaaag aaaaaagaag agaaacaaaa
    10141 agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg aatctgcttt gtcacaagct
    10201 gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca agcgctacct
    10261 gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat caaagcaaaa
    10321 gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc agaacaagcg
    10381 caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc aatcgcacag
    10441 caacaagcag aacaggcgcg acagcaatca gcagctgaac aggcctctca gcaggcacaa
    10501 gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac cggttcaaaa
    10561 taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag cgaagctcaa
    10621 gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag ttatactata
    10681 ataataaaat tcttctgaaa cataaaaaaa tagaagatgg cgttccagat agtaaaccaa
    10741 aaataacagt ttacacgtcc atttcgtcag cttctctctt ttttcattta gtctgttccc
    10801 tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac ctctttttct
    10861 tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga atgtttatgt
    10921 tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct gccttttaaa
    10981 tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac tttttctcta
    11041 gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc gattggtcgt
    11101 ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat ggccaaagaa
    11161 tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat accgataaac
    11221 caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt ttttgcctct
    11281 tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt gaccatataa
    11341 ggagtacagg taagcaaagt cacgagatct ctgccttttt ggatacccaa agaatctact
    11401 tcatcaggta agaccacttg gattttttct acttgatacg ccaatgtctt tccattgatc
    11461 tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa ttttgcttct
    11521 gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt cggataagaa
    11581 ctttcttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg atcgaatata
    11641 ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc cataaaaaag
    11701 gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa actgcctatc
    11761 cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt ttcctgctgt
    11821 ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat gatcatttgc
    11881 tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat cccgatccca
    11941 acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt attcatcatg
    12001 tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt gacctgagta
    12061 ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag cgaataccaa
    12121 gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt tatttatcgt
    12181 atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt tgaaatagat
    12241 cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc ccataccacc
    12301 agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag aagttgttgg
    12361 atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg cgtagccttc
    12421 tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga ttgaaaattt
    12481 cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga cttcatctga
    12541 ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta atttaacagt
    12601 tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt gcgtgttcac
    12661 atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag taatcgtgtt
    12721 atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat tggcttcatc
    12781 tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta taaaagtagt
    12841 ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa ctaaagtata
    12901 attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg tgaatgtcaa
    12961 cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac cggtcacgaa
    13021 ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta cccctgaaaa
    13081 gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag catataggtg
    13141 aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt tggcgacgtt
    13201 tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc atcagattta
    13261 cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc gaagggtgta
    13321 gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat gtcatatacg
    13381 ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt tccgttgttt
    13441 atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt aacattttct
    13501 ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac gaaaggaagc
    13561 agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt cattttattt
    13621 ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct gttgaaagga
    13681 ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt tttggaaagc
    13741 ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc gtttttccat
    13801 tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga tgaagcggat
    13861 cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata tatcctttag
    13921 gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca gaaaatgttc
    13981 catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc caatgattta
    14041 tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga gcatctgcta
    14101 gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt tggatggcaa
    14161 gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca aatgtcgttt
    14221 tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt ccagcaaacg
    14281 aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt tgtgtgtgaa
    14341 atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct agccataact
    14401 gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc tcaggaattg
    14461 ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc ttattctcta
    14521 ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga tacaagtgga
    14581 tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca aatggtgctg
    14641 atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca gcgtcacgac
    14701 cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg tctactgttt
    14761 ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt tttctttgtg
    14821 cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag acatcatata
    14881 tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg atatttattc
    14941 cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta tgcaaagtaa
    15001 atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag accagtagga
    15061 taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc atcgctgttc
    15121 acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa agatcagtcc
    15181 taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga gagtcggatt
    15241 gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta cacttccttc
    15301 gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa ctgttagggt
    15361 ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa ccgaaatcga
    15421 gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg cgttagcttt
    15481 atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact tgggatttgc
    15541 tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc cagcattaac
    15601 catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg ccacttgtac
    15661 tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg ggacacctgc
    15721 ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt gcttaatcgt
    15781 atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag ctagtttagc
    15841 tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag gcgcagttac
    15901 ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga tttgatcaat
    15961 actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg catatttaac
    16021 gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga ttgatactgt
    16081 tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg cgtcctttac
    16141 gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt ggttctttgt
    16201 ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat tagtcgtcag
    16261 gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa tcatctgtcc
    16321 aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg tcaccgtatc
    16381 gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat agcccgttac
    16441 tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg tagtcccgta
    16501 cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg tccgattcgc
    16561 attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc ccttaccatc
    16621 aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg ctaactgtat
    16681 accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg ttgagggtgt
    16741 aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc cagcggtagc
    16801 cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg tggagccaat
    16861 aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg ttacgccttg
    16921 taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc catctttggc
    16981 atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag tggccgcaac
    17041 agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg ctgcttttgc
    17101 tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg ttgaggttgt
    17161 ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat agggtggact
    17221 atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa cgtttacata
    17281 aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca taatgggatg
    17341 taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt gacatatacg
    17401 ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata aaaaatcatc
    17461 aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga tgtatagaaa
    17521 gtttggggct ttttttctct ttttattcta aggttat
//
LOCUS       DAFGZE010000059        16958 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.061, whole genome shotgun sequence.
ACCESSION   DAFGZE010000059 DAFGZE010000000
VERSION     DAFGZE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.061"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..611)
                     /locus_tag="LTU54_002101"
     CDS             complement(<1..611)
                     /locus_tag="LTU54_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBL8296804.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYL"
     gene            complement(859..1695)
                     /locus_tag="LTU54_002102"
     CDS             complement(859..1695)
                     /locus_tag="LTU54_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HBL8296805.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(1706..2635)
                     /locus_tag="LTU54_002103"
     CDS             complement(1706..2635)
                     /locus_tag="LTU54_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBL8296806.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            2827..3180
                     /locus_tag="LTU54_002104"
     CDS             2827..3180
                     /locus_tag="LTU54_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HBL8296807.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(3254..3910)
                     /locus_tag="LTU54_002105"
     CDS             complement(3254..3910)
                     /locus_tag="LTU54_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBL8296808.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            4136..6070
                     /locus_tag="LTU54_002106"
     CDS             4136..6070
                     /locus_tag="LTU54_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBL8296809.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(6156..7379)
                     /locus_tag="LTU54_002107"
     CDS             complement(6156..7379)
                     /locus_tag="LTU54_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL8296810.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7373..9271)
                     /locus_tag="LTU54_002108"
     CDS             complement(7373..9271)
                     /locus_tag="LTU54_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HBL8296811.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(9265..10383)
                     /locus_tag="LTU54_002109"
     CDS             complement(9265..10383)
                     /locus_tag="LTU54_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HBL8296812.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(10466..11299)
                     /locus_tag="LTU54_002110"
     CDS             complement(10466..11299)
                     /locus_tag="LTU54_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8296813.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(11292..12209)
                     /locus_tag="LTU54_002111"
     CDS             complement(11292..12209)
                     /locus_tag="LTU54_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8296814.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(12436..13560)
                     /locus_tag="LTU54_002112"
     CDS             complement(12436..13560)
                     /locus_tag="LTU54_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL8296815.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            13743..14651
                     /locus_tag="LTU54_002113"
     CDS             13743..14651
                     /locus_tag="LTU54_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8296816.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            14792..16405
                     /locus_tag="LTU54_002114"
     CDS             14792..16405
                     /locus_tag="LTU54_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL8296817.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         5353 a         3303 c         2804 g         5498 t
ORIGIN      
        1 cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc
       61 tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct cattagataa
      121 aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt
      181 tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta actttggatg
      241 ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt
      301 cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt
      361 tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg
      421 atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat
      481 tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat
      541 tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata
      601 cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac
      661 ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt
      721 tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa
      781 tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg
      841 tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac
      901 tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc
      961 tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg
     1021 catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg
     1081 gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct
     1141 gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata
     1201 gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat
     1261 ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt
     1321 ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc
     1381 tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc
     1441 tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg
     1501 ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc
     1561 caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata
     1621 ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat
     1681 caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg
     1741 caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta
     1801 gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga
     1861 acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa
     1921 aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa
     1981 ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac
     2041 gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca
     2101 gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat
     2161 tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa
     2221 gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct
     2281 gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag
     2341 cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa
     2401 cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa
     2461 aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata
     2521 cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga
     2581 tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt
     2641 tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac
     2701 aagttgctct gtaaattttt acacaactct tattttttac caaagcactt caattgactt
     2761 tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga
     2821 ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc
     2881 gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga
     2941 ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca
     3001 ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa
     3061 ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta
     3121 gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa
     3181 tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa
     3241 tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga
     3301 aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg
     3361 gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact
     3421 gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt
     3481 ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc
     3541 gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat
     3601 ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg
     3661 tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac
     3721 catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat
     3781 ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta
     3841 aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat
     3901 gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata
     3961 gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta
     4021 ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta
     4081 attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat
     4141 attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat
     4201 acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa
     4261 atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa
     4321 aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac
     4381 agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg
     4441 cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt
     4501 aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa
     4561 tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat
     4621 cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca
     4681 ccctgacatt ttgattctag acgaaccaac gaaccattta gatatcgaga cactttcctg
     4741 gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta
     4801 ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta
     4861 caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa
     4921 ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct
     4981 tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga
     5041 ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa
     5101 gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac
     5161 cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc
     5221 gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg
     5281 tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga
     5341 tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga
     5401 aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc
     5461 aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa
     5521 caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga
     5581 agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg
     5641 atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt
     5701 ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga
     5761 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
     5821 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
     5881 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
     5941 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
     6001 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
     6061 tgaaaattaa cgaaaaaacc aaaacagaaa ccattcatgc tgttttggtt tttctttttt
     6121 tattttatca aacaaaaatt ccatatttac ctttttcacc aataagaaga ccaattacta
     6181 gtcattctca ctaaagcttc taaataatag taatcacccc aaatacaagg tgtatttacc
     6241 ccattatttt tgggcttatt ataaacagac tgtgttaaaa agccatcttc ttcttccgtt
     6301 gtagcatatc tttctttcaa ttgtcgcata attttccaaa cagcagactg gtataagttc
     6361 ttttctgttt tgtctaatag acctgattct attaattcta ataatccaca aaccatgatc
     6421 accatactag atgtatcttt ttcttctctg ctagaatcat caaagatcaa atcccaataa
     6481 caaatggtat cttctggcaa ccgattcata aaataattgg ctaatatttt gcttaaatca
     6541 aaatattttt gttctttcaa ataacgataa cttaatgcaa aaccgtaaat cccccatgct
     6601 tggcctctag cccaacagga attatcacta taaccttgat gcgtacttcc atatcttggt
     6661 tttccatctt tgacatccat ataaaatgta tgataagttg aaccatcttc tcttactaaa
     6721 taatctgctg cattttgaat atgagcctga gcagcttttt tatatttttg atttccagtt
     6781 tcttcactcg cccaatacaa taatggaaga ttcatgcaac aatccatgat cattctacct
     6841 tgttgtgaag gatcactagg atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa
     6901 cgctgaagta atgtttctgc agcatcaatt gaagcttcct tcatttttcg atttcccgtt
     6961 attttataat ctgctacggt tgataaagaa tacaaaaaac caagatcgtg atgattagtg
     7021 ataatttgac agtcaatcct gtggataaaa tctatatctg ctttttcagc tgtttgttga
     7081 aataaaggat cacccgtctt ctcaaataac aaccaattaa ttcctgtcca aaatccttct
     7141 gtccaaccaa tattgtctct taatggataa cgtcctttta taggacactc gcttgggaat
     7201 tgatactgac tgttactgat acgtttgatt aacctctccg tgtgtctgcg acaacgctcg
     7261 atctcattat tccattcttt ttccattaat tctttataac attcatttat ccatttttct
     7321 ggatgtatta tctcttcaaa catcacttta tcaagcattt ccccaccagc ctttagcata
     7381 attttagtct ttttttattg tttttatttt ggatagttag cccggttgtc ccatcaaaat
     7441 caattttggg acgattgttc catacttcta aactataatc ccgatcttga tgagcaacca
     7501 ctcctgttcc gagccaatgg gttccttttc caaatgtacc ctctaatcca ggaataaaag
     7561 tgttttccca tgtcatcaag tttgtattgg gcaaacccat aatagctgtg gattttcgtt
     7621 taatagtatc gtcacttaaa cagataatac ctgaaaagcc agcttcactc accctatagt
     7681 tttccttgtc ttcttctatg attattcctt ctttctcagg agaacattca atagcaaacc
     7741 ctgtctcata tagaacatat tctgtatcta attctacttt gtgtacgcga atctgccagc
     7801 cttcaaaagg aataagccaa gttctgattt ttgcctgtaa agtaggttcc catgtagagg
     7861 caagtacctc tttcttacat acaatgtctc gtacattatt tcgaacgatg aactgtccat
     7921 aggttttgat gctctcattc ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc
     7981 ctagcattga atccattgcc atttctgctt tcaattgatt cgctcgcggt atcgaaaaac
     8041 caaattgact agaataagca aattttaaat atttttcatt ggtcaatgta tggaaatttg
     8101 gccctaattg tcccgcattc aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat
     8161 tggcttctga aagaagttta gttccttcac gaataggcat aggcatttct ttagccgtcc
     8221 aatactcatg gtcttcaggt agcgccaaca ataaaaaaat cttattcagc cataatggag
     8281 ataacgtact attatagggt tcagtcaatg tcaattgagg gtaggtataa ccaatattca
     8341 ggataccttt ttcatcaaat atttcttttt gcatccacca tcttatattc cgattgacta
     8401 accccttgat ttcacctaat ttaaaaggca aaagatcggc ccacaacata gctgaccaaa
     8461 agcttgaaac tgcaaaacga taaatcatgc ttcgaccaaa aggcacattt gcaccatctt
     8521 ccgcaaaaaa atgaatatag tctctagaaa aatcaatagc tcggttctta tatatctggc
     8581 taatgggctt ttctggttct agtacgctat aaatcaaacc ataataatga aatgcaaaag
     8641 gattataata atcttggcga cctcttgaac tgtctcgata ccagccattt tgaagataac
     8701 aattttctat attatcaaat gcttctttca gtgctgattc tttaactgga taaccaaatt
     8761 tttggataat tgttccgaca attatcttaa aaaattgcca attaccatct gcacattcat
     8821 attcaaaaat ttgcgagaac caatgaataa tctggctttt ttctcctgta tctaattttt
     8881 tccataataa ttgttcatga tgatataacg ctaataacat tgcgggcatt tctaccatac
     8941 gctggtctct ttcatgtatt tctccccaat aatcgggatg ggaaggatct gttccagctt
     9001 ttattcctga tagaattaat ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg
     9061 ctccccataa aacccgagag aatccttcca ttcctgcaat tttatttcca tatcctaccc
     9121 cttgatgtat ataatcaatt tgtgcgccac ttgctcgata aaacggctgg agtggttgat
     9181 ttatcttttt aaataagtca aagtaatcct gtctggtcct taatagtggt actggatgtt
     9241 tcattgtatt ttctcctctt tttactacca aatagctaat tccgactgtt ctaatcgaag
     9301 taagccttca ataaaaaaat aatcagaata gatcagtggc acattataag tcagttctct
     9361 atggtatgct tctgctccgc ctccaatgat cccatcttca tccatattcc aatttgcata
     9421 gtgcttttct gtcttttgga gtatattgat agcagcttca tgatacattt ttgatcgtac
     9481 agaggaaaca tatcctgcta attccagaag accgcatgca gcgcatactc cagcactggt
     9541 atcaatattc gaatctttta cagcaaaatc aatgagagga atataaccag ttttttcgat
     9601 tttctgaata aaatttactg ctatcttttc tgctatagct agatagtacg gattttttgt
     9661 gtaacgataa cttaacgcga atccatataa agcccatgca tttcctctgc tccaacaaga
     9721 attctcattg tatccttgcc cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa
     9781 ttctgcgata tgcccggttg atccatcttt cctcactaga tacttagctg ttgtatctgc
     9841 atgctgccga gcaattttag agaacctcgg atcaccagtt tccctcgaag cccaatacag
     9901 taatggtaaa ttcatcatac tatcaataat catccaacca ggtttatggt cattccatgc
     9961 tactaaatag ttccctgaaa tattatagcg tccagcaagt accgtagctg catgtaaccc
    10021 tagttgtcga ctatgtttgt tttgagactg ttgataatgt gcaatagccg atggtaagaa
    10081 taaaaaacct acatcatgat gaacgtctga aaaatggtac agggcagatt ctaataattg
    10141 ttcttgttgt attgctgcct ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca
    10201 taaaatacca ctccaaaaac cattggtcca ccagtcgatt cctctgtcct ttattacgtc
    10261 tgtatatttt ccgttgtaag aaatatatgg aattttattc cccattctgt ctaattccgc
    10321 atctatcttt tcagaagcta cattcaaaat ttttcttgac cattccttcg tctgttcatt
    10381 catggatatc ccgccattct tttaattttt atgaatgtgg agttgctcct agaacgaact
    10441 agatggttaa cattcgttat tttttttact tatttgcagc tccaatgtca ttctccttaa
    10501 atttagactg cgcccacaca taaatcaaga ccattgggat cactgaagtt gctaaaatta
    10561 gcataattgc gccataatta attggctcac ctgtactaga aaccgtcata tttttgataa
    10621 ttaagggaac cgtatattta tctttatctg aaagcatgac taatggaaat agatattgat
    10681 tccaacttcc cataaagata agtaaaccac tagtaataat cgtaggtgat aaaatcggta
    10741 cagcgatttt cgtaaaaatc acccactcat tggcaccatc gattctggca gcctccatca
    10801 gtgctgtagg aaattgactg ctatattgtc gcatcataaa cactgaagtt gtactggcaa
    10861 tcgaagggat gatcacacct gcataagtat tcgataatcc taaacgactc ataatgtaaa
    10921 ataaaggaat caatgtggta aaaaaaggca ccatcctaga tagcataact gtattgaaca
    10981 acagtttatt ccccataaaa cgatactttg ctaatgcata acccgccatt gtagaactgg
    11041 ctgtagctga aactgtactg attacagcaa taacaatcga attccaaata atcaagggat
    11101 aattatatct cgccatgatg gcttgccagt tttttaatag catatcccca aacttgaaat
    11161 tgattccgcc acttaaaatt tcttgattac tttgcgttga agctacaatc atatataaat
    11221 atggcatcaa aaatattaag gcaccaaaca caataatgac gtacgtaaaa attctgcttt
    11281 gtatcttttg tttagtcatt tttatcacct ccgaatttaa attgaatagc cgataatata
    11341 acagatacaa acaccatcgt ccaggcaata gcagaggctt ttccaaaatc aaccatttca
    11401 aaacttgttt tatataaatt gactcctacg accaaagctg aattttctgg tccattggaa
    11461 gtcccaaaga atatatttgg gatattgaaa ttacccaagc cattgatagt ggctaaaact
    11521 gtggccatta aaatgattgg ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat
    11581 ttcgatgctc catctatttc agcactttcg tatatctctt tagaaatccc ttgaagacca
    11641 gctaaaaata aaatagtgtt atatcctaca ctaagccaaa tcatcgtgat gatcattgat
    11701 attcgtatcc aaaaaggatt tgttaaccac tgtacaccgt taattcctag cgcattcaaa
    11761 aatccattga ataacccagt cggattgaaa aaagtcatga atacggaagc tacagccacc
    11821 gttgatgtaa tggttggaat ataatagaca gttctaaaaa atccttttcc ttttaactgt
    11881 gctgaattca ataaaacagc aaagtaaata gctaatagca tcgttattgg aattgatcct
    11941 gccatgataa tcaaaacatt cgtgtaagat ttaataaaca atggatctcg gaagatagct
    12001 agataattgt caaatccaac aaattcaaat gagtagccaa ttttcataaa actaaaagca
    12061 aacgagataa tgatgggaat caaaatcgtc acaataaaga aaataacaaa aggtgtgacc
    12121 aatccataag cacttttttt atgagacgta tctgttgctt ttttcttgct gttctttatt
    12181 gtcttgcttg gtaacgaaga ttgcatcatc gttaagtcct cctaatattt tttaatcacc
    12241 agttaatttt acgccgtact tatgagcaaa attttctgct tcttttagat atgaattttc
    12301 aaaatcacct gtttgccaat agttatatgc agcggtagtc aaataagacg tagcatctga
    12361 ataagtggct ggtaaaacag tatttgatgt attttcattg attttattca atagttcata
    12421 gtatttttgt cctccttagt agtcaaactt tttctcagct gcttctgttt gataggccaa
    12481 tgagttagat gtagccacgc ccagctccag tgcgttggct aagcattctt catctgtcag
    12541 cataaatgta agaaattgtt gtgccacttg tggatttttg gcatctttag ggacataaaa
    12601 acttgatcct cctgatactg caccttttcc tggattttct tttgaaaaag gaatcagtgg
    12661 agccatgcgc catttgtcag atgcatcagg aaaattcaaa gacatattcg tagcatacca
    12721 gccgccagca acaaacattg cagattcttg aaatgctttc tcagcatctt gtgcaccgta
    12781 gaaatgtaca atatctgagt cgatcatttc tttgattaac tttgcagcat gtttcgcttc
    12841 tttagtattc agacggatat ttccttcttc atcaagcaaa gattgattct gctgggctag
    12901 catgttgata aacaattctt ggtccccaga ttctggcaag gcaattccaa aactaccagt
    12961 tttttctttt aatgcttttg ccacctggat atattcttcc caagaactga tttcttcaaa
    13021 atctacacct atttcagcaa atttttttgg ttgataataa caaagtgctg gggtaaaatc
    13081 actagcccaa ggaataatcg tttcattact cgtttgttga cgcaaaatat tcaatttttg
    13141 atcataaaac tgatcgccat atttgtcata aaatccatat tcatcagcac tataaaaagc
    13201 atcttgaaat ttttccagca cacctgctgc tgaagtgtct gccatggaga taatatccgg
    13261 catatcttcg tgtgttacca attttggggt gatcatttgt actgttgctg ttcccgaagc
    13321 tacattcaca atttttaagt caatatttgc tccagttttg cggttataga tttttgcagc
    13381 attttcaaca tagtttgtca gtttgccgtt tgcgacatat acttctaatg tagtggcttt
    13441 ttttatcatt ggatctgcga tgtttgctgt tacttcttcg ttagtttttc cacatgcact
    13501 taagctgaat aatagggtaa tgtttagcaa agctatcacc aatttgtatc tttttttcat
    13561 gtctatccct tctttctttt caagttctta tataaaacgc tttcacttat gtatattaac
    13621 ggaattagta acacaaatat ttatataaaa aaatatttta cttgtcgttt tggcttttca
    13681 tttttgttat aatagtcatt aattataaat agaaagcttt ttatataaaa agagaaagga
    13741 aagtggttct tttgttaaca aacaaccaat cctccaatct tgtcaaaaaa acactaacct
    13801 attccataga tacccatctt catttatctg ctgctcccta ttttcgacta ctaataaatg
    13861 aaagcgctaa tttatatgct gaatttgcac ataaatctat agaactagat aatccttccc
    13921 ttttattcat tgataaacag caagaagtcc aaatcttttc tactcaaaag gaaacacaaa
    13981 cttttatcat tatccagttt tattttgatg agcaattatt agcacatatt acgggacaat
    14041 ctttttctat agatcaagaa ataacatcta tcattacttt tctgaaaaat gaacagttca
    14101 aaattgatta tctaaaacat agtaagccag ctatttccta tgatcaggaa atcaattctt
    14161 ttttcatact atgccaacag atgagcattc atttgctttc cttattagtt acttattgtc
    14221 tgcaaaaaat tcaattgtct tccttaccaa ggcattatca acaggaactt gctttattga
    14281 caataccttc aaatgagaaa gtcattccta aaaaaaagca tattctttat tcagatgaat
    14341 tagttgatcc aatcatacga tatttagaag aacatctttc agaaaatttt ttgcttgaac
    14401 aattagccca aatttttttt atcagtccag ccagcttaaa aaaacatttt agaaaagtga
    14461 atggaaaaag tgttattagc tattttaaag aactaaaaat ggaaaaagca aaacgtctcg
    14521 tgctagaaaa tgaattaacc tatacagaaa ttgcagatat attaggattt cattcgatcc
    14581 atcatttttc ttcagctttt aaaaaatata cagggttatc cccttcaaag tattatctat
    14641 ccttaaaata gaaaaaaatt tttaggtttt atccacaaat tatttctcta tattttctgt
    14701 ctattcaaaa taaacaataa ttttccggca caattatttg ctgtttttta ccataacata
    14761 gtacgatcaa cgtagaaaag gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc
    14821 tggcggtcct tcatttgcca ctcgtttccg tcgattgaat gaagcgcacg aaatcattat
    14881 ctatgaacgc ggagaaaata tttcttatgc aagttgtgct ttgccttatt atttaggagg
    14941 tgtgatcact gaccgtgact cgctgatcga acgtacacca gaaatattga aaacaaaaaa
    15001 caacatcgat gtctttacta aacacgaagt aacagcaatc gatccttcta ctaagcgatt
    15061 aacagttaaa gacctatcca caaatgaaga aataaaaaca gattacgata agttgatcat
    15121 ctcttctggt gctagaccag attatccgga tattcccgga gtttttgaag cagaaaacgg
    15181 ctttgtcctc cgtagtgtga cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa
    15241 tccacaacat gtcgtcattc ttggtgcagg tgttatgggt ctggaattag ctgagaattt
    15301 caagcatcgc ggcttagacg tgactttaat cgatcaattg ccacaagtcg ctttccctta
    15361 tgatccagaa attgctaatt tagtttatga caaattgctg aaagaaggat tagccgttca
    15421 tttagaaaca agagttactg agatccgtga taaaggtcga gaaatagtat tatcagatgg
    15481 ctccgtcctt tctgctgata tgctaatttt tgctgttggt gtttctccga agaatgaagt
    15541 ggtgaaagca accggcatac aattatcaga tacaggtcag atcattgttg atgaccagtt
    15601 acaaaccaat ctgccggaca tctatgcgat tggcgatatt atcgaaacga ctagtgtagt
    15661 gactggtcag ccgatccaaa gtatgctttc cagtgcggcc aatcgtcaag gacatatgct
    15721 ggcagatatt ttaaatggta cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc
    15781 aaaaatcttt gatcatacag caagttatgc gggaatgaca gaacatgaat taaaagcatc
    15841 aggcataaca aattataaaa ctgtttttat cactcccttt gaccatgcct atttctatcc
    15901 agaagctaca agattaaatc taaagctgat ttttgatgca gatagcggtc gtattttagg
    15961 tggacaagca tttggagaaa aaggtgtcga taaacggatg ggagaacttt ctgtagcaat
    16021 caccggaaac ttgacagtct ttgatttgcc tgatttggag ttgccttact ctccaccgta
    16081 ttctactacc cgtgacccgt tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa
    16141 tatcgtagaa acgatcaaag caagtgatat acccgaaaac gatttgaaag aagcgttctt
    16201 tttagatata cgtgaaccta ataaagcacc ttctggcagt atctcggcaa caaaaaatat
    16261 tccaatgaat gagttacgag atcgaatcaa tgaaatccca aaagataaaa aagtttacat
    16321 tactttcaga agaggattga atacttatac ttctgcccga atcttggcag gtttgggtat
    16381 caaagcgatt ttgattgaag aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag
    16441 ttatttttac ttgaggagta aaaatgaaaa agtatatagg gttgttggtt ggtatgtata
    16501 tagaatagaa gataaatgtg aagaaattgt gcattatttc ttcacattta ttaggttata
    16561 gtttaagaaa gtttaaacat tgcgcaattt aattatttta gctgtttctt ttcataaaaa
    16621 aacatgttgt gactctgaga caaatcatag tccatagtga tgtctctaga gtaactacgc
    16681 ctaatattac gaggcatcat attaatctat cagcactagt acctaaacta gcatcccttt
    16741 cgaatatttt ttcttatata ataaagtttg ctttcactcc tctaatctca ccaaatcgac
    16801 tggcttgcgt tgtgtgatac taggaactga ttggtttaga acgacttttc tattgtaatc
    16861 atatttttcg ccatcctcgt tgtcttctaa tccttcaatg aatgatccct aatgtgtgta
    16921 ttaaataatt taattttttt gttcctattt atttaaca
//
LOCUS       DAFGZE010000060        16820 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.062, whole genome shotgun sequence.
ACCESSION   DAFGZE010000060 DAFGZE010000000
VERSION     DAFGZE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.062"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..456)
                     /locus_tag="LTU54_002115"
     CDS             complement(<1..456)
                     /locus_tag="LTU54_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL8296818.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEK"
     gene            complement(561..1424)
                     /locus_tag="LTU54_002116"
     CDS             complement(561..1424)
                     /locus_tag="LTU54_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL8296819.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1620..3719)
                     /locus_tag="LTU54_002117"
     CDS             complement(1620..3719)
                     /locus_tag="LTU54_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HBL8296820.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LTU54_002118"
     CDS             complement(3939..5048)
                     /gene="rpoD"
                     /locus_tag="LTU54_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBL8296821.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5072..6913)
                     /locus_tag="LTU54_002119"
     CDS             complement(5072..6913)
                     /locus_tag="LTU54_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBL8296822.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7095..9410)
                     /locus_tag="LTU54_002120"
     CDS             complement(7095..9410)
                     /locus_tag="LTU54_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBL8296823.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9595..10455)
                     /locus_tag="LTU54_002121"
     CDS             complement(9595..10455)
                     /locus_tag="LTU54_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HBL8296824.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLCGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10514..11035)
                     /locus_tag="LTU54_002122"
     CDS             complement(10514..11035)
                     /locus_tag="LTU54_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HBL8296825.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11313..12074
                     /locus_tag="LTU54_002123"
     CDS             11313..12074
                     /locus_tag="LTU54_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8296826.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12100..14691
                     /gene="mprF"
                     /locus_tag="LTU54_002124"
     CDS             12100..14691
                     /gene="mprF"
                     /locus_tag="LTU54_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL8296827.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LTU54_002125"
     CDS             complement(14879..16066)
                     /gene="tuf"
                     /locus_tag="LTU54_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBL8296828.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16434..16790
                     /locus_tag="LTU54_002126"
     CDS             16434..16790
                     /locus_tag="LTU54_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL8296829.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4617 a         3491 c         2946 g         5766 t
ORIGIN      
        1 tttttctttt tcatagcatt cctgacagat tccatggaac gttagccgat gatcttttac
       61 taaaaaatga taacgttctt ctactacttg ctccacttca agtaaaagat cctcttctac
      121 ttcctcgatc gtgccgcagt tcaagcatag taaatgatga tggaaatgtt tggcgccttc
      181 ttttctcaaa tcataacggg ctaatccatc attaaaactt actttatcga caattttcaa
      241 ttctgtcaag atttcaaggg ttcgataaac agtagcaagt ccaatttccg gactcttttt
      301 tttcactaaa aagaagattt cttctgcaga taaatgctct ttctcatttt ccaaaagcac
      361 aagcagagta gcttctcttt gcggtgtcag cttaaacccc gcttcatgta gctgctgttt
      421 tgtttttttc atcgccgaaa tagcattcat gaccatttac tcaaccccaa caatttataa
      481 taattacaaa taaaaaaaga tataacacta tgttaattaa aattattata agctagttaa
      541 gaaaaacatg caagggattc tcaattagat tttttgacta gttttgtcac accgttttct
      601 tgtgtaatca atccttgttt caaaagatta cctaatgcgc gcttaaatgc tcctttgctg
      661 ataccaaaag ctttcatgat ttcttctgga tccgatttat ctgtaaagtt gatttgctga
      721 tctgcagaac gttctaaaat ggttaaaatc atttgtgcat catctgatat agcttcatat
      781 gctcttggct tcaatgaaag attcagtaca ccgtctggtc ggacaccaat cactcgacct
      841 tttatctgct cgcctagacg aggttcatta tatcgttcag aaggatggat aaacccaaga
      901 tagaaatctt ccgttaaaac gtacgtacct gttaatttca agcggtatgc tgttcctatc
      961 acatctttgt ttttcatttc ttctgttcca tatttgctta aagatttgaa tattttttca
     1021 tctgctaaac tgccccaaat acggtctttt gcatcaactc taagggaaat catcaagcga
     1081 tccccttttt taggccatag ctctgtcatt gtcggcaatt catcaagaga tacgacaaag
     1141 tctttatctg gaagaccgat atcgacaaac gcacctaaat cttttcgtgt agcagtaacg
     1201 attccaaaag cgtaatgacc gatacggctt ttaggaattt gtgtagtgat ccgattttca
     1261 tgtttttgat tttggtaacc gaatccttca actgcttcac cgattttgtg ttcaccttct
     1321 gatttgctta agccaaaggt ttgtccgttt ttttggacaa agtagttttt ttcattttca
     1381 tcaataataa tacctgtaaa tatagttgct aataaatcgt tcattttttt ctccctctat
     1441 agtgaaaggt tttcttaata catttctttg ctatcataac ataatgttat ctacttgaat
     1501 agctgagcct tggtttggaa aggtgaaaaa aggagtatga caacagttgt gtcatactcc
     1561 cctttttcga ataaacggtg ttaaacggca ggctcacagc ctctttcatt tgcctaaaat
     1621 taattgctcc catttcctcc gaaccatcca gttttacagt tgatggttac gatgtaaaat
     1681 ggcaaagcac tttctccaat gcctttcatc aatgcagatt ttgttgaagt ggcatataga
     1741 ttataatagc cttccccatt ttcgattctg gctttttcta atctgtaacc tccttcaacg
     1801 atatatccac gttggataca tgtagcaatc actttgcttt ggacacctgg tgcccacgtc
     1861 catgctggat tagatgcatc ggcaatatca cttgctctgg cagatttgat tggctgattc
     1921 gctgagtttg aagaggaagg ggtcgaggat gccgcttctt gctgtgctgc ttgtgcgacc
     1981 gcagcttgtt gagcggcttg agctgcagct gctgcttgag cggcttgttt tgcttgttct
     2041 tcagctgctt ttttcgcttc ttctgctttt ttcttttcct cttcttcttt tttctcttta
     2101 tcagtgagca tcttatctac ttgtttcaat tgttcattca actgagcaga tagatctgta
     2161 tttttcactt tttctactgc ttcttttgcc gtcttgtatt ggtcgcgtgt tgctgaatcc
     2221 actacttttc cgtctttaaa gactatagct gttttttctt tagctgtttg caattcagca
     2281 tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt ttccaaaatc tgtgttatcc
     2341 gtttcgattg cttggatctc tttatctgct ttcaacttga tgtcatctgc tagatgatca
     2401 tcgacgatgg caggtgctat gaaccactca ttcacttttt ggatatctcg gatcttagat
     2461 tggatatctt ggtaagttgt ttctaactct gaatatcctt tttcattctt aagactggat
     2521 aaatcatttt ttagttttga taaatcttga tgaatcatat ctgcacgaat aaattgatgg
     2581 tgatcatctg tataaaaggc agcaagtttc tcatgaagtt cttccatttg ttgctgtctt
     2641 tttgcttctg ctgctacttc ggcttctact ttttgatgat ggtcataata agcccagctt
     2701 cctccaccga gtagaagtac tgctgctaaa acgagataag gtactttttt tgattttttc
     2761 ttttgcgctt tatctggatc tgttttttct aagtttgatt cttgatgtgt tttttcagta
     2821 tctggcacga ttgttattga tttcgcttct gcttcaaaca gcttaggctg gttctcttct
     2881 ttttctattt ttgcttcaag atcagtaccc tctttgctta cagattgatt ttcagcatcg
     2941 gattcactat ttatttcatc gggctctgtt gactttttgt ctgtgttttc gagtatttct
     3001 tttttctctg cttccggcag tttattcgtt ttttcctttt ctaattgttt ttcagtaaat
     3061 acttcagttt tttctgtgat atccgttttt acgtctttta tttttactgt ttctttacct
     3121 gtggcgtttt ctctatcaga gtctttttca gtttcttgtt ccttttctgg ctctactttg
     3181 ttttcctgtg caattttttc actgatcatt ttgagcagct ctttcgcgtg ctcttctttt
     3241 gtatcttcac gatgtgcttt gatataagcg gatagtatcg aattttcttc ctcattatcg
     3301 acgatttctt tcagttcagc tactcttctt ttttgttttg attcaagcgt ttcttcttga
     3361 ttctttcctg attcttccga agtctcatga acagttgctt tcttctgttc atttttctct
     3421 atattagtta cttgatcgtg agcaggtggt tcttcgtctt ctggttctgc tttttcgaat
     3481 aattccatta cagattctag aggtaaatct ttcaattccg accattcgat tggatcgttt
     3541 aattctgtat caggaaagac tgcatcttca gttgatttat cctgcatagc tgcatctgta
     3601 tttgtttctt caattgattc ttttatttgc tgatcttctg cagttatttg atgaccgcag
     3661 tctggacaga tagtattctg ctccattttt ttcgatccgc aatttggaca ttttttcatc
     3721 acttagcgat cggctccttt actaggttta cgctaccatt taatctatca tattctatac
     3781 gtcacgccaa tagcttattt ttaggattta cttaaaaata atgaatgaat ttccttccta
     3841 tagaaaaaaa agcttgagac tgcgtagtct caagcttttt ttcttttaaa ataaaccaaa
     3901 cagttttact gatttctctt ctggtcaata gtccacgctt attctaagaa atcttttaat
     3961 tgttttgaac gagaaggatg gcgcaatttt cttaatgctt tagcctcgat ctgacgaata
     4021 cgttcccgag taacaccaaa tactttccct acttcttcca atgttctcgt ccggccatca
     4081 tctaaaccaa aacgtaagcg aaggacattc tcttcccgat cagtcaatgt atctagtaca
     4141 tcttccaatt gttcttttag taactcataa gctgcgtgtt ctgctggact tgttgcttcc
     4201 tggtcttcta tgaaatcacc taagtgagag tcatcttctt caccgattgg cgtttcaagg
     4261 gaaacaggtt cttgcgcgat tttcaagatt tctcggactt tttctgttgg aagatccatt
     4321 tccgcaccga tttcttctgg cgtaggttct cttccaaggt cttgcagcaa ttgacgttgg
     4381 atacgtatca atttattaat tgtttcaacc atatgaactg gaatacggat cgtacgtgct
     4441 tggtcagcga ttgctcgagt gatggcttga cgaatccacc atgtagcata ggttgaaaac
     4501 ttaaatcctt tgcggtagtc aaatttttca actgccttca tcagtcccat gtttccttct
     4561 tggatcaagt caaggaactg catgcctcgt cctacataac gtttagcgat acttactact
     4621 aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt ggtcgccttc ttcgattttc
     4681 aatgctaacg ctacttcttc ttccgcagtt aaaagggaga cacggccaat ttctttcaga
     4741 tacatacgaa ccgggtcatt gatttttaca ccggttggcg cagaaagatc ttctgttttt
     4801 gcttgttcag cttttttctc agcactcttc aagctgtgaa ttgatggatc accattttca
     4861 tccaccacac tgatacctgc gtcttcaatc ttttggatca atttgtccat ttcatctgca
     4921 ttcaaagtga atggtgtagc aagctgattg gataaatcat catatacaac ttgtcgtttg
     4981 ggtttgtttt ctttgatgaa agctgctact gctgcttcat acgttttttt atctgttcca
     5041 tttgccataa aagaaggccc cccttgagta gctatttggc ttgttttaac tgtttcgtta
     5101 aattgatgat ttcaatcgtc aattcaagct caagctgttt gtttccagtt cgtctggctt
     5161 cttgctgcat ttgttttttt aatgttattt catcagctaa gccagatttg ctgatcacat
     5221 ctagcaagtc ttggatttct cgtggcgaac tttcttctgc caccggtaaa tattcgattt
     5281 ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc taaaaactct gcaaggttga
     5341 attcataatg ttgtgagaca tatgcatcga ataaaaaata aacttctgca tatgcatcat
     5401 gaataaactg aaattctcgt tcttttaaaa tattacggac agaaacttct ttaaatgcac
     5461 gatataatag aatctgctct gccttttcta cttgcgtcaa aggtctcttt ttccgtgcag
     5521 tttcttgtct cgtaacctga gtgtgttgac tttcctgagg ttcttgacga ttttgctgat
     5581 ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc tctgccgatc tgaaactctg
     5641 ttgacagttg agatagatac atatcttgtt cgattggtga aacaactcgg ctaagttcaa
     5701 ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt atctaaattt ttcccttgtt
     5761 ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc atgagccact aattcttgga
     5821 aagatgcttc tccatactta cggagatatt catcaggatc aagcttttct ggaacgacta
     5881 cgacagaaag tgctaatcga ctgttttgcc gaagcaattc aatccctcga ttggttgctt
     5941 caaaacctgc gttgtctccg tcatagcaaa agaccaattc tttcgtcagc ttttccattt
     6001 gttggatctg ttgagctgtt agacttgtcc ccatagaagc taatccagtt ttgataccag
     6061 cctgccatgc agcaatcaca tccataaacc cttcgaacaa caaaatttct ccgtttttcc
     6121 ggatatcgct tcttgcttta tcaagattaa ataaaacttg tcttttattg aacagttctg
     6181 tttctggact attcaagtat ttgggctgat ctttttcctg ccctttttca ggtgcaagcc
     6241 agcgtcctga aaaaccgatc gtctggcctt gagaattacg aagtggaaac atcactcttt
     6301 gataaaaacg atcagacata cggccatcat catgcgtgac aaacaatcca gagcgttcta
     6361 acaattcatt tgaaattgat tcattcttga atacttgctg aagaaaagaa cgctcattag
     6421 gtgcaaaacc tatgttgaat tcttcaatta attctaaaga caacccacga ctttgcaaat
     6481 attctaaagc tggctgtcca gcttttgtat gcaatagcat gtgatgatag atttccgctg
     6541 ctttttcatg agcagcaatc agtttccccg tagcagattc aggattttgg acagctatag
     6601 tacgctgacg ccattgatct tctaacggta tctgttcgaa gtctgccact tttaccactg
     6661 cttctggaaa acttagacct tctagttctt gaagaaaagt aaacacgttt ccgcctttac
     6721 cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac agaaaaagac ggtgtacgtt
     6781 cttcatgaaa aggacacagc cccaagtaat ttttaccaga ctttttcaac tggacatatt
     6841 gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt ttcgataacc tcttgaggaa
     6901 tccgttgagc cattttttca ccccctgctc tattgatatc ttttgggttt aacagaagat
     6961 atttcaggag aataaccggt aaaaaatcgc actaactgaa atcagtgcga ttttaaagga
     7021 cacaattata catgtttgat tgtatcatat ttcattgctt tttcaagtat aacacatttc
     7081 tatctcatta agcatcaatt tgctactctt ttcagtaact gattttgaat atcagccttc
     7141 cagtacaata ctcgaagaac aatatttacg agcatgaata caaagaagta tcctacgctc
     7201 tctcccaacc ctaagtttac ttccaaaatt ccatggaaca gcattgcagc tacatatatc
     7261 cctacgatcg tcgttactgc taggaagatg cgcaatgggt tgaagggata acaagctttt
     7321 acaactgcca tgcaactgat cccaatcagc aagtaataca tcaaagtagt agtttcagca
     7381 tttccccatg cgaatgtttt tccaagtaga tacaccgcaa tgatattcac tactacaagc
     7441 aaggcatttg gtaaagcatt aataagtgct gtaggcaaat aacggtaagt gatttttctt
     7501 ttatcttgtt caaaagataa aaagaaggaa ggataccctt caatcgctaa atcaatcagc
     7561 gtgatttgga tagggataaa cggaaatcca attgctgtaa cggcacatat aatcgataaa
     7621 ataaatgaat aaatcgtttt gataaaaaag atgcctgaga ctttcgtcac attgttgacc
     7681 actcggcgtc cttcaaacag gacttctggc aatgtagtaa aatcagagtc caacaatacc
     7741 agattcgcaa tctgacgtgt tgctccatca ccttctgcca tagcaatgct gcaatctgct
     7801 tctcttaacg ccaaaacatc attcacaccg tcgcctgtca ttgcaaccgt tctgccgctt
     7861 tctttcaatt catttaccag caatttcttt tgctgcggac tcactcgtcc aaaaaccgta
     7921 tagcgatgag cagcttctcg cacttccatt tcttcagtaa tttgagaaag atcgacatac
     7981 gcatcataat tcggtaatcc tgcacgtcta gcgatattgg agacagtaaa aggattgtct
     8041 ccagaaatca ctttcagatc cacaccttcc tcatgaagat aagctagtgt ttcttgagcg
     8101 ttttttcgga ttggatcatc gatttcaaac agagcaaccg gctcaacaaa tgctggtttt
     8161 tcttcgttca attgctgatt tggagcgatc cccaacataa gtacgcgata gccattcgct
     8221 tgggctgttt ttagactgtc cggaagccga tcttcttcca ccaaacgttc aggcgctcct
     8281 acgtacacga ctcccagttc tggaaattcc atcgcgcccc atttgcgatc agaagaaaat
     8341 gcgactttgt ttgtcacatc ataccgatta gaaagctcaa aataatctct catagcccgc
     8401 atagtgatgt tgttatcact gctttctgcc agatacgtcc ccataatttc tgggatggct
     8461 ttctcgtaca gcggatgtag tgcttctacc cgctggatac tcattttccc ttcagtaatg
     8521 gtccctgttt tatccaagca taatgtatcg acatgggcca gcgtttcaat tgaatacatg
     8581 tcttgaacaa ggattcgttt ttttgccagt ttggttaccc ctgtcgttaa tgcaatactt
     8641 attagcaata ccaaaccctt tggaagcatg cctaacaaag ctgctgcaga tgcaataaca
     8701 gaaattttga tagaagcatc gcggatgaac agtgcttcca aaaatagtat gatccccaaa
     8761 ggaataatca cgaaactagt gaatttggat actttgcgaa tcgaatctac aagttcagaa
     8821 cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga cagcataatt atctgcacct
     8881 actcggataa cttcagccat gacagaaccg cttgtcaaaa agcttccaga aagcaattca
     8941 gctccagttt ctttgacaat caaatcagat tcgcctgtca aaagagcttc atttgcttct
     9001 gcccgtccag atataacctg taaatcggct ggtatctgtt ctcctgctcc gatttttacc
     9061 aaatctccta ataccagctc tttagtagaa atcgattgtt ctttaccgtc ccggatcact
     9121 agtacttctg attgggaaac aatcgataat ttccgtacaa gatttcttgc atgtatttct
     9181 tgataaattc caataacgat attgacgaca ataattgcta aatacgccat gttggaatac
     9241 gcacctacaa ataacagaca aatcccaata ccaaagttca gaaaattgaa taacgtcaat
     9301 atattatcgc tgaatatttg ttttgtactt tttgatgcat cttcctggta gtcattttgc
     9361 tggccagaag ccattttctg aactacctct tgctgtgtaa gtccattcat tttctttttc
     9421 tccattttcc gccaactttc aaaaataatc acgtgtttaa gtctagcgtg ttccaagatt
     9481 aaaaacaact tgtttttata aaatttatag aaaaacacac gaatgatttt acaaaaaaga
     9541 tgccaacctt tttgtttgaa tcacccgtgt gctgttttta ttatcaatct atctttactt
     9601 atattgttcc tccaaacggc tcatccacca acctagtgct gtaccggcta taaaaagaac
     9661 aggacaagct agtaccccag caactcccaa tcccgcgtaa tcggtaggaa tgatcataat
     9721 cgttgaagca aaaactacac ctaaaatgaa atggaatagc ttgctatatg cctttgagaa
     9781 aatgtaatcc atgatttttg acaatccaag aacacaaaga atcccaccaa ttccgatcgg
     9841 cacaatcact tcaagtcgta cttctttaaa tccgtcagcc atcgctttat acaatcccat
     9901 atacaccaaa aaattagaag ggctcaatcc tgggacgatc atacccaaac caatcaatcc
     9961 gccggcaatc agccatgttc caaaattctg atcgacttgc ccgtcaaata attgagcgcc
    10021 gcataataaa aacaaataag ccagaacaaa agtaacaacc atgatgatca catcagaagt
    10081 atctcttcct tttttccctg cttctttcca taacgcagga acagttccag cgatacatcc
    10141 gacaaaaaac cataatatcg ttgtttcata cgcacctaat aaaaaactga ctaaaaaaga
    10201 aagtagaaag atgcctgtca ctccgcctag accaacaggc agaaaaaaca aaacattttc
    10261 tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc cgctcataca tgccaaagac
    10321 agctgctaat gcaccaccgc tgactcctgg caagataaat cctgaaccga taaacattcc
    10381 cttcacaaac cgtaacatcc agtctttggt tgtgtacccc acttcttgat tttccatttt
    10441 ttgccttttt tccactattt gcgcactcct tctttcattt aattttattc atgattgaac
    10501 atttcctgct attttactta aagacttttt cataggtctc tattattttt tcattcattt
    10561 tgacaatatc aggataaaac atgttacctc cattatgata gagatagccg ccattgtaaa
    10621 gcaaggactg gatccgccaa tatcggtatt gtttcccttc ctcatttcct aaaagtgggc
    10681 tcagcacatc tttagaatac gcttctgcta attctactgt gttttctccg ccatgatttt
    10741 ttacataggc aatataatct ttcccaaaat tatatgcttg gacagctgtc cataaatcgc
    10801 atccttgttt ctttgcttct actatcattt ccgccaaata tttgacacct tgatcaatac
    10861 tgtcagctgg attatcaatg atctcagctt ctccatgcag actttccgag ctctgcatga
    10921 gatctgtccc ttttcctttt gattcggtca atattatcgc cttgatcaaa ttctcgtagt
    10981 tttccatatg ataatttttt gcagctgttg ttatttctat ttgatagctt tccactcgtt
    11041 ttaccgaacg ataagttctt cctcataaaa acaacaccgc accaataacc gtcagaatga
    11101 tcatgtaaaa aagaatacgg ataatagata atcgttttcg cttcatttca tcacctagtt
    11161 attttgacat ctttttttat cttactattt ctttatcttc ttggctattt cttccttgat
    11221 ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt ttttgacatc ttacgaaaat
    11281 gatcgttgtt tttcagtaaa aacaattgcc caatgactga tataatcgtt atactttaca
    11341 cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa aatgccagat gggagcgaaa
    11401 tttactatga aaagagcggt caaggcttcc ctctcttttt actgcatggc aatgacggca
    11461 gcggccgctt tttttcagaa caggtccctg tactcgaacg ctactatacc gtgtatctag
    11521 tagatagccg aggacatggt cgttcgacaa acgaagcatc tatgttgaat tttcagttaa
    11581 tggccgagga tctgaatacg atcatgttgc tggaaaaaat cgatcaggca gattttcttg
    11641 gatttagtga tggagcaaat ctagcactgg tatttgccag cagcttccct aaaaaagttc
    11701 atcgattgat tttaaattct ggaaacacgc ttgttaaagg cgttcgtttt tctgccagag
    11761 tcatcagtaa tattcattat gcatgggtat ggttactctc tttatttcgc ccaagtcttc
    11821 gcaaaaatct tttagttatc aaacttttgc tgcatgacat cgggcttaca gaaaatgatt
    11881 taaaaaaaat caactccccc actttgatca tcgtgggaaa aaaagatgta atcaagctaa
    11941 aacattcttt gtatatagct aaaactatac caaaagcttc ctttgtgctt gtgaaagaac
    12001 aaggacatga attagctaga aaagatcctg aacgcttcaa tcgagaagtt cttcaatttc
    12061 tttctgaaac ataactatta ggaatagaag gtgatttatt tgttaaaaaa taccatacaa
    12121 tggctgaaaa accacctagg actgttcaaa acaatctttt tgatttctgt cattgtcatt
    12181 attgtcggtg aattgatgtc catcggtaaa actctatcta tccagcaatt aggagaaacg
    12241 tttgccacta tacctgtatg gaaaagcgga ctaatgctat tgattggtct tatctctgtg
    12301 ttacctatgg ttggttatga tattattttg aatcggatgc ttgaacaaaa gcagaatcct
    12361 cgttacctgt ttgagacaag ctggctgatc aatacgatca acaatattgc cggtttcggc
    12421 gggtttgtta gtgtggggct tcgctctgaa ctatatggac aaaagaaaga gagcagaaac
    12481 gtgatccaag cattatcgaa agttttcttg tttttgatgg ctggactttc tatttacagt
    12541 ctgctttctt ttttacttgt agtcttcacg ccagttgctc cttttttgaa gcaatattgg
    12601 atatggttga tcggcggcgg tctatatttt cctgtcgtct ttttatttac tactttcaaa
    12661 aagaaaggat tccttggtgg tacctcactt aaaactcaag gacagctatt gctagtttca
    12721 ttgttggaat ggaccggtgt cctgtttagt ttcctttctg ttggttattt aatggaaatc
    12781 cggattgatc tgtggcaaac aattccatta ttcattgctg cttcagtgat aggaatcgtg
    12841 tctatgattc caggagaaat tgggagtttc gatgtaatga tgattatcgg gttgtccgct
    12901 attggtgtgc caagagaaat agtcgttgtg tggatcttgc tctatcgcct gttctactac
    12961 atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa atatcggttc cacttttgat
    13021 cagcgttatt ctggtatccc taaacaatta gcaacagaaa ttgcgcataa aatcgtggtt
    13081 gtcttattat acttttctgg aatcatgcta gtactttctg ccactatccc gcaagctttt
    13141 acagagtttc gctggcttca tagtttgaac ccattgaaat ttcattttat tatccaattt
    13201 cctagtatac tattgggatt tcttttaatc attatgggca gagggatcgc agcacgtgtg
    13261 aaacgagcat atttgcctac aatcttcttg attgttctag ctttacttta tgttttatta
    13321 agtgatttta gtttcacacc ggtggttttc ttgtctattc tgcttctgat cattcttgct
    13381 tctaaaaacg aattatttcg ggaacaattg atctattcat gggagtggcg tacgatcgat
    13441 ggaatcatta ttggtgcact gtccttgctt tacatcgtta ttggggtata caatctgccg
    13501 gatttccctc atcggcgtca ccattttatt tcctttttct tatttccttc tgaaaagatt
    13561 tggttttcgg gattgttggc catcatcgct gttagtttca tgatcgttct atttgttcat
    13621 ttccttcaag gagaaaagaa acagattggc gaagccttta atgaagaaaa ggccctgaag
    13681 atccttacaa cttatggagg aaactccgat agtcaattga tcttcttaaa agataagcgg
    13741 atgtttgcct atgaaaaaga cggtgaacca actgttcttc tccaattcgc ttgcttcaat
    13801 aataaatgta ttgtaatggg tgatccttct ggtaagaaag aagattttcc agaagcaatc
    13861 gaagctttta tcgaagaaac agatcgacta tgctatttac cagttttcta cgaaacaagc
    13921 gaagagattg ttatgatttt gcatgaattc ggctacgatt tcattaagat gggggaagaa
    13981 gcttatgtag acctgaatag ttttacaact tcagggaaaa aaatgaaggg cactcgtgct
    14041 gtcttgaatc ggatcgaacg agaaggattt acatttgatg tcttacaacc accattttct
    14101 actgagcaaa tgagtacttt caaaaatatc tctgataatt ggttgggttc acgaaaggaa
    14161 aaagggtttt ctttaggctt tttctctgaa gattatctac aaagagcgcc tattgcagtt
    14221 gtcaaaaata cccaagaaga aattgtcgct tttgcaacga tcatgcctac ttacacgaac
    14281 aataaagtag gaacgattga tctgatgcga tatgattcta aaaaagcacc ttctggcagt
    14341 atggatttct tgttcttgca tttgtttgcc tatatgaaag aagaacatat ccaatggttc
    14401 aatttaggga tggcccctct ttcaaatgtc ggaacttcca gaaagagttt tttacaggaa
    14461 aggattgcat acttggttta cgagtttggt tctcattttt attctttcca tggattaaga
    14521 gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt atactctata ttctagagat
    14581 agttggattg catatgttat gattgctttg ctgattattg ataatgcgcc agttgagcag
    14641 acacaaggta gaataagtgg tttgaaaaaa tggatacgaa gaaagtatta aaaactgcat
    14701 accgatagat gaacactctc atgatgaata aaagagactg aagtaaaacg aaagttcatg
    14761 cttctacaat ctcttcttat acaattgaag aacaaagagc ggtccaaaca gaaatcctgt
    14821 ttggaccgct cttttatttt aaacattttt cattaccaaa atattcgagt cgcgtttctt
    14881 atgcttcgat ctctgtaacg atacctgaac ctactgtccg tccgccttca cgaattgaga
    14941 atgtcgttcc gttttcaaca gcgattggat ggatcaagtc aacatcaatc gtcacgttgt
    15001 caccaggcat gaccatttct gtatcttctg gcaatgtgat tgttcctgta acatcagttg
    15061 tgcggaagta gaactgtgga cgatagttgt tgaagaatgg agtatgacgt ccaccttctt
    15121 cttttgtcaa cacgtacact tctgctttga attttgtatg tggagtaatg gaacctggtt
    15181 tagcaagtac ttgtccacgt tcgatatcgt cacgttggat cccccgtaac aacacgccta
    15241 cgttatcccc agcttccccg taatctaacg ttttacggaa catttctaca cctgttacaa
    15301 ctgctttttg tgtttcaggt ttgatcccta cgatctctac ctcatcaccg acacgaacag
    15361 caccacgatc aatacgacct gaggcaacag ttcctcgacc tgtaattgag aagacgtctt
    15421 ccactggcaa tagtaatggt ttatctgtat cacgttctgg tgttgggata tattcatcta
    15481 ctgtatccat caattccata atagcagctt cagcatctgg atcgccttgc aatgctttta
    15541 atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa accatattcg ctcaataact
    15601 cgcgaacttc catttctacc aaatcgatca attcttcatc atcgactaaa tcaactttgt
    15661 tcaagaaaac aatcaggtat tttacaccaa cttggcgtga caataaaatg tgttcacgtg
    15721 tttgaggcat cggaccgtca gttgcagaaa caactaagat tgcaccatcc atttgtgctg
    15781 cccctgtaat catgtttttc acatagtccg cgtgtcctgg tgcatcaata tgcgcgtagt
    15841 ggcgtttttc tgtttcatat tctacgtgtg cggtattgat cgtaatccca cgttcacgtt
    15901 cttctggagc tgcatcaata ctagcatagt cttgaggatt tgctaggcct tttttgccta
    15961 atactgtagt gatcgcagca gttaaagtag ttttcccatg gtcaacgtgt ccaatcgtac
    16021 caatgttaac gtgtggttta cttctgtcat aatgttcttt tgccataatt tacaaacctc
    16081 ctaaataata tagatcactg aaaaatttca gctctcacct ttttattata gtcttttttt
    16141 gactaaatca aaaagaatac gtccgactta ctttttgtaa gattcgaact aagcttatta
    16201 tacgtcaata ttggtcaaag tcaactaaga gcactcgtct gtcttatacg cgaaaaaagt
    16261 accttttata aataaaaaaa tgtgtttttt gagaaaaact tcaagatagt ccttaacttt
    16321 cttttgaagc aataagaaca aaatgaggaa aagtttaaaa ttcagctgtt gccaacaact
    16381 ttcaatagac gaaatcttct atttcgttaa taataaggaa agaggtgaag tacatgaaaa
    16441 aattaattgg cgtacttgtc gttttaggca tatttctcgg cggaagtttt gcagcttatc
    16501 attattttta cggcggcgaa gcttattata caaaaatcac gacgaatggc gaaatgtcag
    16561 ctacccaagc agataacgga gagaaattca cgacttatac ctacaagcaa gcggcttata
    16621 ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga aagagaacat ccattaaaga
    16681 tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg tgtattaagc tgggaagaaa
    16741 taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat cgataattag taaatggtct
    16801 taaaatttta ttagataaaa
//
LOCUS       DAFGZE010000061        16339 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.063, whole genome shotgun sequence.
ACCESSION   DAFGZE010000061 DAFGZE010000000
VERSION     DAFGZE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.063"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(37..705)
                     /locus_tag="LTU54_002127"
     CDS             complement(37..705)
                     /locus_tag="LTU54_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBL8296830.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            839..1126
                     /locus_tag="LTU54_002128"
     CDS             839..1126
                     /locus_tag="LTU54_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296831.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1542..3080
                     /locus_tag="LTU54_002129"
     CDS             1542..3080
                     /locus_tag="LTU54_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HBL8296832.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNAPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3077..4042
                     /locus_tag="LTU54_002130"
     CDS             3077..4042
                     /locus_tag="LTU54_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296833.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4029..5351
                     /locus_tag="LTU54_002131"
     CDS             4029..5351
                     /locus_tag="LTU54_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL8296834.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5414..5518
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5592..6335
                     /locus_tag="LTU54_002132"
     CDS             5592..6335
                     /locus_tag="LTU54_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296835.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6350..7342
                     /locus_tag="LTU54_002133"
     CDS             6350..7342
                     /locus_tag="LTU54_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBL8296836.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7354..8091
                     /locus_tag="LTU54_002134"
     CDS             7354..8091
                     /locus_tag="LTU54_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296837.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8081..8803
                     /locus_tag="LTU54_002135"
     CDS             8081..8803
                     /locus_tag="LTU54_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBL8296838.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLKEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8887..9510)
                     /locus_tag="LTU54_002136"
     CDS             complement(8887..9510)
                     /locus_tag="LTU54_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HBL8296839.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9845..10279
                     /locus_tag="LTU54_002137"
     CDS             9845..10279
                     /locus_tag="LTU54_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBL8296840.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            10302..10622
                     /locus_tag="LTU54_002138"
     CDS             10302..10622
                     /locus_tag="LTU54_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296841.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            10763..10954
                     /locus_tag="LTU54_002139"
     CDS             10763..10954
                     /locus_tag="LTU54_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296842.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(11025..11282)
                     /locus_tag="LTU54_002140"
     CDS             complement(11025..11282)
                     /locus_tag="LTU54_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HBL8296843.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            11440..11703
                     /locus_tag="LTU54_002141"
     CDS             11440..11703
                     /locus_tag="LTU54_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296844.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            11727..11915
                     /locus_tag="LTU54_002142"
     CDS             11727..11915
                     /locus_tag="LTU54_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296845.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(11998..12183)
                     /locus_tag="LTU54_002143"
     CDS             complement(11998..12183)
                     /locus_tag="LTU54_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296846.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            12398..12769
                     /locus_tag="LTU54_002144"
     CDS             12398..12769
                     /locus_tag="LTU54_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL8296847.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(12919..12991)
                     /locus_tag="LTU54_002145"
     tRNA            complement(12919..12991)
                     /locus_tag="LTU54_002145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12956..12958),aa:Gln,seq:ctg)
     gene            complement(13037..13465)
                     /locus_tag="LTU54_002146"
     CDS             complement(13037..13465)
                     /locus_tag="LTU54_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296848.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(13525..14283)
                     /locus_tag="LTU54_002147"
     CDS             complement(13525..14283)
                     /locus_tag="LTU54_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL8296849.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            14578..14775
                     /locus_tag="LTU54_002148"
     CDS             14578..14775
                     /locus_tag="LTU54_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296850.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            14881..15363
                     /locus_tag="LTU54_002149"
     CDS             14881..15363
                     /locus_tag="LTU54_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296851.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(15421..16017)
                     /locus_tag="LTU54_002150"
     CDS             complement(15421..16017)
                     /locus_tag="LTU54_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL8296852.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT         5431 a         2677 c         3333 g         4898 t
ORIGIN      
        1 tttctgtaaa ttaaataaag tagtgaaaaa taagaatcag tgctctaact gttcaagagg
       61 atcaagtggt tcaaatgttt ctgtagtctg ttttgcttcc aattggggca aaacagatcc
      121 aatttccaga tacttcactt tattcaatgt ataagataga atagaacagc taagtaatgt
      181 atacagaaaa tgtgtgagat ccaaataaag caaggagaca actaaaatac caaaagtaat
      241 caagaaaaga cttttgccaa tcttaatatg aaaatatttt tcaatggctt gataaatgat
      301 atctgttccg cctgtgcttc cgccgaatcg aaagacgaga ccgcaaccaa tgccagaaag
      361 aatacctgtc atcaaactat tgatcagtag actatctaca gcaaaatgta tatgaaggct
      421 ctcccataaa tgtaaaaaga aaactagcgc aaatgttccg acgatcgtcc ggagaaaagc
      481 gtgttttcca aaaagaaaca atgagataag caataaaggt aaattaaaaa gcagtgaagt
      541 gacagaagga gcgaatccaa aaagattatg caaaagcaaa gcaataccag taaccccacc
      601 atctgccagc ttagatggaa tattgattcc tgtcacagct aatccgtaga ttcctgcgcc
      661 taaaatgatt ccgcacaatt ctgtaaataa atttaatttt ttcatttcta gcctcttttc
      721 ttttgaaaat tttcagcaac ttctttatta tactgactta tactcttact tcaagaggaa
      781 aatgaaaaaa aattattttt ggctgtttct ttttatttat caaataagga gtggaaaaat
      841 gcgagcagca gacttattga caattcttac aaaatcaccc aaaaaaacag agtatcaagg
      901 agtttttttg gctcaagaac aaaaactggt gcctgtgaca atggtccaga taaataaagc
      961 aaatcaattt atctttttat ttgaacacgg caaagcacca cttgctatga aagacatctt
     1021 tctttttttg atgaagaacc gtgagaaagc tgtttattat cagtcaaaag aagaactcat
     1081 tcctctttat ggagtgaaag agatagaaaa taaagtggtg atataagtaa aaataagaaa
     1141 acaagacgtt tatgatgtca aaacaaccat tttttacatt tttggcaaaa aagtgtcatg
     1201 atacagtata atattcgttg aacaggccaa taaaggtgtt ctcccccacg agatttctac
     1261 tttattggct gtttcttttt attaaaataa aagttgtgtg tgtaaaaaag atatagctga
     1321 ttccaagtga caatttcgta tagattttta tggtattcgt agtgaaaaaa cgagaaccaa
     1381 aaagttgatc cgactttgtt gcttggattc tttttttgtc agcatattta gaggtatttt
     1441 tttctttttt ttgttcgtgc tgtaaggtta aggcaagaca agttagttgg gaaaaaggaa
     1501 agaaatgttt atacttaaag aataacagaa aggaaggtcg catgaataat tttatacaaa
     1561 cattaagtga aagaaaaggc gagttgctgg aagctgcatt tcaacatctg tccatttctc
     1621 tcatttcttt gctgattgca gcactgattg ctattccttt agcgatttgg gcagccaatc
     1681 ataaaaaaat ggcagaagtc ttattgcaga tcacgggtgt gctccaaacg attccttcac
     1741 ttgccttatt aggtttactt atcccatttg tagggatcgg aactgttcca gcattgattg
     1801 cacttgttat ttacgctttg cttccgattt tccaaaacac gtatattggt ctagctgaaa
     1861 ttgacccgtc tattgaagaa gcagcggtag catttggtat gtccagaatg agacgcttgc
     1921 tgaaagtaga gcttcccatc gcattacctg tcattatctc aggtatccga actgccttag
     1981 ttttaatcat cggtacggct actttggcag cattgatcgg agcaggtgga ttagggacat
     2041 ttattttgtt aggtattgac cgaaatgcac ctgttttgac attgattggt gcgatcagtt
     2101 ctgccttgct tgcaattata ttcagcggac tgattcgctg gttgcagcat aagaaacctc
     2161 gatattccat cattacttta gcagcagtgg tgtgtttatt tggcggcgtt tctttatatc
     2221 agtctgcctt gtttaaaaat gaaaccatca cgattgctgg aaaattagga gcggaacctg
     2281 aaatcctgat ccaaatgtat aaagaattga ttgaagacaa cacaaaaaca gaagttgaat
     2341 taaaaccgaa ttttggcaag acgagttttt tgttcaatgc actggatcaa ggtcaaatcg
     2401 atatctatcc tgagttcaca ggtactgtcc tagaaagctt ggtgaaggta cctgaagcaa
     2461 tgaaaagtgc tgatttgaca aaagaaaaaa catatgacga agcagaagaa ttattgaata
     2521 agcaattcga tatgacatta ttaaaaccaa tggcttacca aaatacgtat gccttggcag
     2581 tcaagagatc ttttgcgaac gaacatcaac ttaaaaagat ttccgatttg gcagctatct
     2641 ctgatcagat caaagcggga ttcacactgg aatttattga tcgttcagat ggatataaag
     2701 gaatccaaga agcatatggc ttgaattttt caagtgtaca aagcatggag ccaagtttgc
     2761 gttaccaagc aatcaataat ggagatgtca atattgtgga tgcctattcc acagatagcc
     2821 agctcaagga gtacgatcta gtgactctag aagatgatcg agggttattc ccagcttatc
     2881 aaggcgcacc gctgatgaaa aaagattttg ctaaagaaca tcctgaaatc gttaaagcac
     2941 tgaataaatt aagcggaaaa ataacagagg atcaaatgat tgatatgaat taccaagtca
     3001 atgtcgaaaa gaaaaagccg acagaagtag cacgaatctt cctgaaaaat gaaggattag
     3061 taaaggagga agatcagtga ctaaattaat cgagttcaaa catgtgcaaa aaaagtatga
     3121 tggaaagtat gtcatcgatg acctaaatct ggcaatcaaa aaaggagaaa tctttgtttt
     3181 ggttggtcct tccggtagtg gaaaaacaac tactttgaaa atgatcaatg gattatctaa
     3241 accatcagca ggagacatct acttcaaagg aaaatcgtta aatgaatata atctgcagaa
     3301 aatgcggtgg aatatgggat atgtgctgca gcaaatcgct ttgttcccga ccatgacagt
     3361 caagcaaaat attgaagtca tccctgaaat gttaggttgg gaaaagcaga aaagagcaga
     3421 ccgtgtagat gaacttcttc aaaaagttgg cttatctcct gatatttatc gagatcgtat
     3481 gcctagagag ttgtctggag gagaacagca acggatcggg atcatccgag ctattgctgc
     3541 aagcccagat gtgattttga tggacgagcc atttagcgca ttagatccga tttcaagaaa
     3601 ctcattacaa gaattggttt tgtccttaca cgaagaattg ggcacgacca tcgttttcgt
     3661 tacgcacaat atggaagaag caatcaagct aggtgatcgt attgcattta tgaaggatgg
     3721 agaaattatc caatgtgaca caccagagca gttattgatg aatcctaaaa acgactacgt
     3781 tcggcatttc tttgatgaac cgaaacagac gaaagagtgg cgagtagaag atttagtcgt
     3841 caatggatat ttcttaaatg aaataccaga aaatgctcgt caacaagttt gctttgatac
     3901 gcctatgaaa gaagtatatt cgcttctttc ggtgtatcca agcattgtga tagtagaaaa
     3961 gcaaagaagt attgggtcgc tgacaagtaa agaaatcttt gcttttttaa gtcgagagga
     4021 ggaaggaaat gaaaacatat gatgcaatca tcatcggttc aggtgtgagc ggcttaagtg
     4081 ctgcttatgg attgaaagaa gcaggtaaaa cagtactagt ggtcgaagaa gatctatggg
     4141 gcgggacttg tcctaaccgt ggatgtgatc ctaaaaaagt cttgttaagt gcagtagagg
     4201 cacgtaatcg ggtaaaacaa ttgagtggaa aaggttttaa cgagatccca actgccaact
     4261 gggaagaact tcaaaaattc aaacgaacat ttacggatcc agtcccagaa agccgaaaaa
     4321 aacaattagc agaagctgaa atcgatcatc tttcaggaac cgctcgtttt ttggatgatt
     4381 catctattga agtcaacgaa gaagtcttcc atgcggatta cttagttttg gcaaccgggc
     4441 aaagacctac gatcttacca gtggagggaa aggaatactt gaaaactagt gcagactttt
     4501 tatcattgcc tgtactgcct aaagaaatca tttttatcgg tggtggttat attgcttttg
     4561 agctagctac gattgccaat gctgcaggga gcaaagtaac cattgtccat cataatcaac
     4621 ggccattaaa agaatttgag gcgtctttgg tagaggaagc cgtccatcaa atggaagcat
     4681 ctggcattca gtttgctttt ggtgttgaaa cccaaaagat catttcagaa ggtactcgct
     4741 atcgtttagt cggaaaagag acggaattag tagcagatat gattttttgt gcgactggga
     4801 gacagccgaa tacagaatct ttagcacttg agcaagcgaa tatcgtcttt gataaacacg
     4861 gaattgcagt caatgattat ctgcaaacga gcaatccaaa aatttttgcg tgtggagata
     4921 ttgtttcaag gaaaacccca aaattgacac cagtcgccac ttttgaagga aattatgtag
     4981 ctaagcggat aacggatgca acaagcgaac ctatcaaata tccgattata cctacgatcg
     5041 tctacgccag tcctaaactt gctgaagtag gagttacaaa aagtcacgcc tcttcgtcag
     5101 atcaagtggt agagatggat cttaccagtt ggttcaccta tcatcgtgtc aatgaaccag
     5161 ttgctaaggc tgaattgacc tttgaccagc aaaattattt gattggtgct gcagtgatta
     5221 gcgagcaagc agacgaattg atcgatgatt tgacactcgt catcaatcaa aaattgacta
     5281 aaaaagagtt ggatagctat atcatggggt atccgacatt ggcaagtgat ctctcctatt
     5341 tattgaaata atcagcatga taactaaagc gcttgttgct ttttccttct tgtgctacac
     5401 tttaaaaaat gaatacctgt ttctggggat gtcgtagacg acatgagagt aagttgtgaa
     5461 cttagaccca ttgaacctgt cagttaatac tggtgaaggg agaaacatag cagacgcttg
     5521 aagcgggtta ctatgtcgtg cccatttcaa gcgttttttg ttattcatat tagaagaaag
     5581 gaggtaacaa aatgaagaaa aatctttatc cagcagtcag tatggcattc attttaatta
     5641 tttggcagat tgttgtcata gtttggaaaa tacctgcctt tattttaccc ggacctgctg
     5701 ctgtattttc tgcattagcg gcagatgcac aagtattact ttcccattct gtcacgacat
     5761 taaatgaagc agtgcttggt ttggtaatag ctagcgttct ttcttttatt actgccttgg
     5821 caatggatta ttggaagttt ttagaaatgt ctttttatcc gttactagtg atttcacaaa
     5881 tgttgccaat catggtgtta gggccattac ttaccctatg gtttggtttc ggtatgcttc
     5941 caaaagtgat ccttgttgtc ttgataagct attttccgat cgtagtttct ttttcggata
     6001 cattgaggaa agtatcaaaa gagcaaatca tctttttgaa aacgatgggg gcagatacag
     6061 ggaagattta tcgaatatat aaaataccag cgggtttatc cggcttcttt tccggtttaa
     6121 aagtggctgc tacttattgt gtcagcggag cagtcgttgg agaatggcta agcgctcaag
     6181 caggcttagg gtattacatg atacgcgtaa aaaacagcta ccagatcgat aaagtttttg
     6241 ctgcgatcat ttgtgtcatt ctattgagtt tattgctgaa tggcgcttgt tcattattga
     6301 aaaaagggta ttatcgtatg ctttatattt tttaagagaa ggggatcaaa tgaaaaaatt
     6361 atggctttta ttaccgctac tgttgctttt atccgcttgt ggcactgcaa aagaaacaag
     6421 tagcaaacaa gaaataaagg atttgaaaaa agtgacactt gttttggatt atgtaccgaa
     6481 tactaatcat actggcatct acctagcaaa agaaaaaggc tattataaag aggctggttt
     6541 aaatgtccaa atcatcgaac caggagataa ttcgacaagt attggtctag taggtgcgga
     6601 caaagcacaa tttggtgtaa gttatcaaga agatgtcact tatgctcacg cagatggaca
     6661 gaatatacca gtcaaagcaa ttgctacagt gatcaaacat aatacttctg gattcgccac
     6721 cctatcagac agtaacatcc attcaccaaa agattttgaa ggaaaaacat atgcaggttg
     6781 gcaaagtcca agtgaagaag ctgtcttaaa agctgtgatg gaaaaagacg gcggggactt
     6841 ttcaaaactg acaatggtag gaagcaatgg agaagggcca gaaagcttag gaaaatcctc
     6901 agatatccaa tggtactttg aaggttggga tatgatcaaa gcaaaagaag cgggtattga
     6961 actaaactat atcccattga aagaattaga tgaacggtta gattactata ctccggtaat
     7021 tattacgaat gatcaactga tcaaaagtga tccagaattg gtacagtctt ttatggatgc
     7081 cacgaaaaaa ggctatcagg aagctattaa ggatccaaat gacagtgcta aactgttgca
     7141 aaaatatgca aaagaaaatg atcggacgtt tttagaagaa tcacaagcat tcttgtcaaa
     7201 aaattatacg gatgatcctg agaattgggg attgatggaa gaaaaagttt ggcgcaatta
     7261 tacagctttt atgcaggaaa acggattgat caagcaagat gtaccttcgc aagaactatt
     7321 cactaatcag tttataaaat aggagtgata gatatgtcaa tccaaatcag ccacttgaat
     7381 gtagtcattg aaaacctgaa aatattagaa gacatccatt tcactatccc taaagatacc
     7441 tttacatcga ttgtcgcgcc gagcggagcc ggtaagtcaa cattgctgaa aactttaaca
     7501 ggagtccagc ctatgacttc tggaacgatc aaagtagacg atcaaaaagt aactggtctc
     7561 catacagcat ttagttatat gccgcaagaa gatatgttac ttccttggct gagcgtctat
     7621 caaaacgtta cgttatatca aaaaatcaat catctaacaa tcaatgaaga acaagtaagg
     7681 gattatctgg atatctttgg gttaaacggc tatgaacatt ttttgccgga acaattatct
     7741 ggcgggatga agcagcgaac ggccttgtta cgaacaatca tgaatccttc ttcttacttg
     7801 cttcttgatg aaccatttgg agcgttagat gctatgacca gagggcagat gcaagactgg
     7861 atgttgaagc tgcctaaaaa agcaaagcgg actactttac ttgtgacgca tgatatcgaa
     7921 gaagcaattt atttatcaga caggattctt gtgctttccg cccgtccggc acatgtgatt
     7981 gctgagattc aagtgcctga aaaacgacgg tcacgtgaat ggctactgca gcaatcagag
     8041 ttgaagcaaa cgatttatca gttattggca ggtgaattag atgttaagtg atgctcattg
     8101 tcatttagat gggagtcaga gtttagcact ccttcagcag gaacacacga ttttgacgat
     8161 catcaattgt gattcgccag aagaatggaa ggaaaacaga cagcttgcag caagtaagac
     8221 tcaagcctta agttacggta tccatccttg gaaagcagat agctatacgt ttgaacaagt
     8281 tgagcctttt ttgaaaaaag caaggatcat cggtgaaata gggttagata atatttggac
     8341 gaatgtaccg atgactacac aaaaaaaagt atttgagcga cagcttgctt ttgcagccat
     8401 aaatgagaaa cctgttgtac tgcacacaaa aggatgcgaa aaagagatct taaattatat
     8461 ccaagcttac cctaatcact atttgatcca ttggtattct tctttggact atcaacaaga
     8521 ctatattgat ttaggctgtt atttcagcat tggtcttgat ttgaaaaaga atccagcagt
     8581 ctggcaacta gctaaagcag tcccaatcga ccacttactt attgaaacgg atggactaca
     8641 tgcagcagag tgggctttag gggggatttt ttcagaaaaa gattatgtct cactgcttaa
     8701 agaacagtta aagacaatcg ctgagatcaa agagctatca gttgaagaag ttaaaaaaaa
     8761 tacttatcaa aacttggaac gtttcattag attaggtaaa taaaaaagag atgtgacaca
     8821 actagtgtcg cacctctttt tggctttctt tgtataaaag taaactgttt gattaataaa
     8881 tgaatcttag taccaaccgt ttgcttccca gaatgctttc gccgcttccc aagaaccata
     8941 acgtgaagta acatattgtt ctgctactct ttcttggttt gcagcagagt agtcaccatt
     9001 taagtaagaa gaatctaatt ggtaacgtcc gatgtaacgt ccgttcgttg ctgtgtaaga
     9061 accgcttgat tctttttgtg cgatccattc ttttgcagag cttgtgcttg ctggagtagc
     9121 agcagtttct gttacagcag gagcaggttg tgtttcagca acaggcgctg cttgtttttc
     9181 cgtttgaacc gtttcttgta caacaggttg ttcagcttgg actggtgctt cttgaacggc
     9241 tgcttgaggt tcagcagctg cttgtgcaga gccttctgtt gggattgtta attgttgtcc
     9301 tgagtaaatc aggttaatat cagaaattga atttgattct gcaatggcat tgatcaagga
     9361 gttatcacca acgtattttt gagaaatcgt tgatagagta tcacctgatt gaacggtata
     9421 agcttcgtcc gcatgagcag tcgttcccat gaagaatgca gcgccggcag ccaaagttgt
     9481 tccaaaaagt aaagttttaa gtgatgtcat gaaaaatctc ctttattcgt taaaaagttt
     9541 tatttgttgt gttcaacgag gaatacttta acacgtgaaa atatttcata ggtaaaagaa
     9601 gcatgacaat ttatgacaaa tccacgtttt ttgtaacaga atgatgttcg ctcgatgtga
     9661 aaaatgatga ggaatttcac ataatgcaga aataatggga ttagaacagg tgttctgatg
     9721 agaaaagaaa tgtataaata acttgaaaga aattgctttt gttcatcatt ttatttacat
     9781 attgcgtata ttttttgtat ttttgcttga gatgttacaa taaaagaaaa aggaggcggt
     9841 tccaatgagt aaaagatacc agaacatcat ggtagcagtt gacggatcaa gacaatcgat
     9901 caaagcgttc gctgaagccc ttgacttagc aaaggacaat gaagcccact tatttatcgt
     9961 gtcaatcatc aacaaagttg agctgaccca tagtgcctat gccttttcaa aaatctatgc
    10021 agatgaaaaa caaagaacag aagtagccat gctgaaaaaa atcaatgatg ccaaagagta
    10081 cggcattgct gagatccacg caatagttga aactggagat ccaagaaatt tgattgccaa
    10141 tgtcatccct ggacaagaaa atattgattt gatcgttatg ggagcgacag gaaaaggtgc
    10201 tatacaacaa gcattagttg gatcgaccgc ttcttatgtc gtatcacatg ccccttgcag
    10261 tgtgttggtc gtgaagtaat gagataaggg aggaatagaa aatggttcgt cctcaagaag
    10321 tccgtgcgcc aaaagaaaaa atagaaattt tagcaattat tgaagacggc acacaaacaa
    10381 agaagggata ctcgatcgct ttagtaaagt ggaaaggaaa aaaaggtgtt gctctgagat
    10441 gggatggaga taaccaacaa gataaaggat tccctgtcac agctaacggt tatcatccag
    10501 cgtggtttgt tctgcctgat aaatttatgg aattatacag ctatgattat gcaagagccg
    10561 cagcatcaat caaagcatta gataaattaa cagaagagca gaatgaaatg gaggaaaaat
    10621 aaaaaagacg aaccaatcag cctttagaaa actgattagt tcgaggagta aaaatgaaaa
    10681 aaggttattt gggatgaaac tagaataatg cgtataactt aaataaaact taaagcaata
    10741 aaatggcaag agggtgaggc aaatggaagc aggaaaagta ttacaggaag taaaagcact
    10801 atgtgcagat aaaaaatatg aagaagcgaa aagcctgatc gaaaagaaca aagaaaattt
    10861 ggaagaaaag tacccgatcg cacaagaatt tatagaatta aaacaaggaa cgatccttca
    10921 ccgattcaaa catttttttg gtataacaga atagaaaaaa cgattaaaga tcggcagaaa
    10981 tttgctgcat ctttaatcgt tttcttttta aacatgcgag ggattcaatt ctgattctta
    11041 tataaaaaat agatccaaat aataagaaaa attactgaaa gcgtcaattc gatgatgtgt
    11101 gttattaata cagacgttgt ttcccaatgc ctaaaagaaa aaatttgata agtaaaatat
    11161 acagcaggta aagaggccag taaaactgaa aacttctttt ttgtccattt tatgtaatag
    11221 aaaagaatgc ttatcataat aaaaaaagga atataaaaaa caaattgacc gatcgcgatc
    11281 atactattgc ctccgataaa taaagtccat gatacaaaat aacactttgt gcgaaatggc
    11341 gcaagcagtt ttgtggattt atccggtaaa atctgtatcg attttgaaat tagaggagaa
    11401 acttgctata atcaaagtga aagaaggagt gggattttca tggatcatgt agtgttagaa
    11461 atcgatttgc atttgatcaa taatattcgg gtgatttact atgttgtaag taacagtgtc
    11521 gaacaacgtg tactgacgaa taaaataaat ggtattttag caaagaaaga cgttcaccga
    11581 ttcaataatg gtgaaggaag ttattatagt atcccagtag aaaaaattat ttatacgaca
    11641 gtcaaagtca gagaagatct agataccaaa aaagcatatg aacctatttt tacaacttat
    11701 tgattgacag cagatggagg aaaaaaatga atccatatga acaacttatt caagatttga
    11761 tcgatggaaa aatcgaaaag attgatgtaa aaagagaaga attaatggct tttcgagaag
    11821 cgtggctgaa gttggagaat cgtaaatatg tggtgggaaa agcctcactg aacggcaatg
    11881 tgacttatca ttacgatccg acacgtttat tttagtatga agtattcaga aaagaaccaa
    11941 aggaggacga tgcagcagca ttgtcctcct ttgattttac aaaagtgaaa gttttactta
    12001 tttgaataga tttttaaatt tgtttttgac actgtcgata tttacatctt ttaaaagatt
    12061 ttgggctttc tctttataag gtcctaattg ttctttatgt tcttcgataa atcgttttgc
    12121 gccatcgaaa ttgccgttct taatcatttc cttgactttt tgaaataatt cttcttgatt
    12181 cattttgaac acatcctttg ctatcaacat gattttttac atggacaaaa cgagaaaaac
    12241 tgatttatcc acacgtcaat aatatcaaat aacgatcatt tttactaaaa aaacactcga
    12301 ttctcgcttc taaagtagga gaaaaatctc caacttggga cggatggaat ttctttaact
    12361 gacccatata ctgaatatat aacaaggagg tagaaagatg aaagtgatta aatatttgat
    12421 tggactggcg gcgattagtg gtattttgct atttggactg aaatattata ctgaaggaag
    12481 ttatggagaa atccctggta tacttgatca gctaaatcca ttagttgaaa aaggagaggt
    12541 ctatgtaaag acacaaaaac cagaggaagt aaatgaatat ggtacagcaa ggtatgtgca
    12601 gaaagctgct gatgccaatg ggaaagaacg aacggttgaa tttaatggtc tttctgtgtt
    12661 aaaagaaaat cattatttga aaattagcaa taaaggagca cacatagaaa cgtatgagga
    12721 agttcctaga gaaaaagttc cggagaaagc attagcaatt attgggtaaa tcagtttttg
    12781 tattaagaat acgcccgatt ttattattca taatttaatt atgtaaacta aatacaagta
    12841 gtaaaaataa tacaataatc cccctgcaaa aagattgttc ttttacaggg ggttcatttt
    12901 ttcagcaagc acatcaaatt gggatggcag ggttcgaacc tacaattgct tgattcagag
    12961 tcaagtgcct taccagtttg gctacatccc atttattcaa caaaataatc atagcacttt
    13021 cctaaaaaaa cgcaagttat ttttcttgat cggatagttg ttgctttact cgtaaaaggc
    13081 ggcgtactag tttcttttct aatccagcag gaagggcaat cagttctgct actgacaaga
    13141 cggtgtccaa taactgatat tctttgacag gttcattcat aatatgatag atgtttgcta
    13201 aacgttcaat atattcaaag cgagtatact ctgttcgagc aattagaaat ttcaataaat
    13261 cggtggcttt ctcgtactct tgatctgcgt aataaacaag agattgacga tacaatttga
    13321 tttccagttc attgagggta gcaggtctgc gacgctcgtt ccactcatta atagagactt
    13381 taccttgaac atttcccttg cagtcacgga caaaaataat cggaatttca tttggatcca
    13441 tttttttatg tgtgaatttt tccattatct acgctccctt caaaggtttg gtatcttgag
    13501 tataagggat ttttgtttga attactagct tttctccaaa gttagcatct gtccacccat
    13561 tgcagtgaaa atcgccgaaa aatcgttgat cggtactgct cgatttttta caccataggg
    13621 atcgttgaca taaatatttt ctttgtcata tccagtgact acacaagcat gaacaagagg
    13681 agtgactctt acttctccat tatttgtggt ccaagtcata aaatcagttt catctgggac
    13741 ttcaaaattg atagtcacag aagtccagac aggatttcct ttagatagtg cttttttgat
    13801 atcatcgagt tgagcatcgg tttttggaaa agtgtgataa tccgtttgga cgatttgctg
    13861 agcaagggag gcaactgggg gaacatagac gaccatagcc cagtctccgc cactgatatc
    13921 accaacaaat ccttcattag gatcgccatg agtcccatct gcattgaaaa cagggacgta
    13981 atccaattgc tcagctaact ggtttttagt tgtttcataa ccataaaaag ataaaagcat
    14041 agaaagcgaa gtgacttcac agccattttc taaagaagga gaatcaaact ggctctctaa
    14101 tggaacatct agtaaaactt ctgctttctc ttttgtcttt ttcgtttttt gtgttgaact
    14161 tttttgtgca gtgttcattg tatgttctgg agaatggata tgtaggtaat aacttgtacc
    14221 gcttaataaa agagcagctg tgccccctag aaacaatagt tttttgaacg tttttttctt
    14281 catttgaaat ctcccttttc agctgtttat ctttcctatt ataaatgtat ttttattgga
    14341 aatggaagta ttatatttta atatgtagga aataattagt ttttaaggaa tatgttttaa
    14401 ttttttgaaa aaaattcttc taaatacaac agctgtttta attgtgaaaa cgaaaacagt
    14461 acgctataat gcctgtaagg ttgattttta actgtaaata aaaatcaatg agttaaatct
    14521 ccaaagaaat agcagtggta atgtggagaa aagttccagc agtttaggag gattttgatg
    14581 acggaagaaa tgattaattt aggagaacaa tatgcatgca aaccaattgg ttttacaaaa
    14641 acggtcattg gtgaagttgt atcaaaaatg acaaactgcg ccgtggtcaa agttgcccaa
    14701 tgtgctgcag aggaccaaga attattagat gaaaaagcaa gcatggttgt tgcaaaatat
    14761 gatacatttg aataaagaaa acaattggac gtgcggcgtg ctgcaagtcc aatttctttg
    14821 agaaggtaaa tcagtattct tttcctattt tctggagaag aaaaagcgag gcgagttgtc
    14881 atggtaacat tctttacggt tctattgttt ttattcgttg ttgtgatgtt tttatttctt
    14941 tggctggttc gaaaagaaat tatttatcgg actgtgcgaa atcgatgggt ctacttggtg
    15001 atacccttct tagtgtcact agtcatttgg tatacactca tcagtcatcc tacagcagat
    15061 gaattggcaa aagggatctt gtcagcaatg atttttatta gttttttgtt ggacagtaga
    15121 ggaatcacag aagaaggact tgttttgaat agctttgaca aaaaaggtgt tcctttttct
    15181 gagatcaata aaattgtatt atatcagcca aaaggttcta aaatcgtaaa aatgaatttt
    15241 ttccgaaacg gatggcgagg accgatgctg aaattttctg catcattaga agaattagtt
    15301 ccatttttgt cccaacgctt aaacgatgag gcagagattg atattatgat tgatccagaa
    15361 taaaaacaga ggttgcgaca agatgtttgt cacaacctct gttttttatt tcaaataaaa
    15421 ttatccaaat acgcggaagc gatcggcatc gttcatgtat tctttttccc cagccggtgt
    15481 ttctggtgaa ccgaatacta attgcgaacg tagtttccag tttgaaggga tattccattc
    15541 ttttgcaact gcttcgtcaa taactgggtt gtaatgttgc aagtttgcgc ccaatccttt
    15601 atcaactaat gctacccaag tattagctgt tgcgattccg tttgattgtt ctgaccaatc
    15661 tgggaagttg tctgcatata attcaaattg ttcttgtaga ccttttacca cgtcagtatc
    15721 tttgaagaat aatactgtac cataaccatt tttgaaacca gctaatttgt tttgtgtatt
    15781 tgggaaagct tctgctggag tcaatgggcg aagtgcttct tcagtgattt cccaaagttt
    15841 ttcatgtgca tctccgaaaa ggataactgc gcgtgttgat tgagcattga aagcagttgg
    15901 gctttcttta attgcttctt tgatcaaagt tgttaattct tcattagaaa gtgaaacatt
    15961 acgtcctaga tgatagattg aacggcggtt tttcaaagtg tcagtaaaag ttgtcataat
    16021 aaaatttctc ctttgatttg cgttttgcat ttttttaatt gattacgaaa tgaagtatag
    16081 caccttacaa aaagtaagta aacagatatg ctcacaaaaa ataagcgaag ttcaaaaaac
    16141 aattttaata agaaaaatcg ttatgtaagg attttcttag ttatcgcttt cattgttgtg
    16201 ttttaggaat ttttgcttat ttgaaaaaaa cagctgatat ataattatta aatctctatc
    16261 ttcttttttt tatgatattg ccattaaaaa ttacttgctt ttatagaaaa ttagtttata
    16321 tttcttaaat aagtttttt
//
LOCUS       DAFGZE010000062        16185 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.064, whole genome shotgun sequence.
ACCESSION   DAFGZE010000062 DAFGZE010000000
VERSION     DAFGZE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.064"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..442
                     /gene="scm"
                     /locus_tag="LTU54_002151"
     CDS             <1..442
                     /gene="scm"
                     /locus_tag="LTU54_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HBL8296853.1"
                     /translation="TSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASS
                     DSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSND
                     NGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(597..1583)
                     /locus_tag="LTU54_002152"
     CDS             complement(597..1583)
                     /locus_tag="LTU54_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBL8296854.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1606..2172)
                     /locus_tag="LTU54_002153"
     CDS             complement(1606..2172)
                     /locus_tag="LTU54_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBL8296855.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2186..2602)
                     /locus_tag="LTU54_002154"
     CDS             complement(2186..2602)
                     /locus_tag="LTU54_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBL8296856.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3059..4993)
                     /locus_tag="LTU54_002155"
     CDS             complement(3059..4993)
                     /locus_tag="LTU54_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL8296857.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5274..6230
                     /locus_tag="LTU54_002156"
     CDS             5274..6230
                     /locus_tag="LTU54_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBL8296858.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6320..7423
                     /locus_tag="LTU54_002157"
     CDS             6320..7423
                     /locus_tag="LTU54_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBL8296859.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7436..8389
                     /gene="menA"
                     /locus_tag="LTU54_002158"
     CDS             7436..8389
                     /gene="menA"
                     /locus_tag="LTU54_002158"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBL8296860.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9203..10549)
                     /gene="gor"
                     /locus_tag="LTU54_002159"
     CDS             complement(9203..10549)
                     /gene="gor"
                     /locus_tag="LTU54_002159"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBL8296861.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFCGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10683..11936)
                     /locus_tag="LTU54_002160"
     CDS             complement(10683..11936)
                     /locus_tag="LTU54_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL8296862.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11942..12865)
                     /locus_tag="LTU54_002161"
     CDS             complement(11942..12865)
                     /locus_tag="LTU54_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBL8296863.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13086..15578)
                     /locus_tag="LTU54_002162"
     CDS             complement(13086..15578)
                     /locus_tag="LTU54_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBL8296864.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15585..16052)
                     /locus_tag="LTU54_002163"
     CDS             complement(15585..16052)
                     /locus_tag="LTU54_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBL8296865.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4755 a         3384 c         2876 g         5170 t
ORIGIN      
        1 tacatctacc acaacaagtg atagcacgga cacgtcagct tcaagtgaca gtacagatac
       61 tacctctacc acaagtgata gctcagattc atctaccact acaagtgata gcacggacac
      121 gtcagcttca agtgacagta cagatacatc ctctaccaca agtgatagct cagattcatc
      181 aacttcaagc gatagtacag acacgtcgtc taccacaagc gatacacgtc atgcaagtgt
      241 ctccttctca aaaacaagtt cggataacag gacgaacttt aacttaacta ataaaacaag
      301 caatgacaat ggaaacaaat caaaaagagc acttcctaaa actggaagtc aatcgaataa
      361 ttggataaca ctcgcaggcg ttatattact tgtaatcggt ttaagaatca cttttatgag
      421 ttataagaaa tcgaaaaggt aaaaaaatat tcaagataaa aaagagcaac gtcgctaacg
      481 gacgttgctc tttatttttg ctttttatca taaatacaag aactacaatt caatttgtca
      541 aaaaacacgt agatttcctt ctgagaaatt tatttacgta tcgaaccttt ccctattcag
      601 tctttgcgat ccaaaatcag ttgggtaagg gaaaagagat tctcttttgt tcgaagagag
      661 gtggcaggta atttctttat ttccttcaaa gccttttctg tgtaagaggc cgctaacttc
      721 ctagcttctt ccacgcctcc tgtatgttca acgattttgc gtacggcgtc gacatcttct
      781 tctgtcattg ccgctttctt ctctaaataa ggtagtagct ttggtttgtt tttttgcaaa
      841 gagtagatca acggtaaaga atagacccct tgacgcatgt cttctagtac aggtttgccg
      901 atttcctggc tctcttttgt ataatctaaa atatcatcga taatctgaaa ggccatcccg
      961 atagaaagtc cgatatctcc tgcttttttt gcgaagcgct cgcttgttcc actttcaaaa
     1021 gcgccaacag aacaacttaa ttggaacagt tcagctgtct tacctgaaat attttccaaa
     1081 tattctttta cagaaagttc ataattatag cgatcatcca tctgccctaa ttcgccattc
     1141 aatacttttt ccatactgcg agaattcaat tggagacttt tcaaggaagt ggagtaatca
     1201 gacaatagtt taaaacagca gacaaataaa tagtctcctg catagacaga aacgacatta
     1261 ccaaactgac tacggatatt aggctgacca cgtcgcagat ccgcttcatc aacgatatca
     1321 tcatggatca gtgtggccgt atggagcatt tcgatagaag ctgccaaagc gatggctttg
     1381 tctcgatctt gctctggtcc aaattgtgag aacaatagct gataggcagg acgcaacagc
     1441 tttccgccgg catggatcgt ttctttgatt gcttgttcga caggtttgtt tttcaggcgg
     1501 acagtttctt ccatccgttt tagtgtttct gtcaattctt tacttaaagc aggatagttt
     1561 tcccacattg gatgtagctt cataaaaaaa ctccttgtgt gacgattatt ttttggtaaa
     1621 cattgggctc catttttgtt tggttccctg ctcataatct ttttggaatc gttgaagtgt
     1681 ctgcacatgt cgtaaaagat aattcgctgc gatcccgatt gcaatccctg ataaaatccc
     1741 taaaactgaa aggactggca agtacaacat gactgtccat gattgtgcaa tgaaagaagc
     1801 catcaaaagc tgtccgacat tatgcatgaa accgccggcc gcactaattc cgatgatact
     1861 gacacgtttt ggtcctaatt gcttgaccaa taacattcca aaataactaa gcaaagcacc
     1921 actcatacta taaaggaatg tggaaatcgt tccgcctagc aaagtagtca ggaatagtcg
     1981 tagccaaagt agtaagaagc tgtctcgttt tttcatggta aaaatggcaa cgatcgtgat
     2041 tagattggct aagcctagtt ttgcaccagg cgcaaaagaa aaaggataag ggatcatatt
     2101 ttccaataaa ccaatgatca ctccttgtgc aacaagtagc gaaatataaa tcattttttg
     2161 taatttactc atggtgtgtg aaggtctaat aaatcagact gtcttcgtca cctccatctg
     2221 ttgaacggac ttcgatcact aatttatgtg gcaaacagac gatggtttcg ccatttttcg
     2281 ttgcccagcc tcgacgtacg cagatctggt catcgcaatc tgcttctttt atccgtactc
     2341 gatcgccatc tatctcgatc aagttataat ctccgtgttc atctttgtaa gtatatgtgt
     2401 tgctctcttc tccagccatc aaagggaatg ttttgatttc tgtgccatcc acacgcagta
     2461 ccgcttcttt ttctgctgtt gcggtgtttt gctgaagacc aaaaatcatg actggtaaaa
     2521 aagaaagaac agtcaaaagc aaaataataa aaacatccca aggtttgagc agactttttt
     2581 cagcaaattc tttaaggctc atttttgttc tcctttttcc agattaactc acccaatttt
     2641 agcataaaaa caagaggttg tgacacctgt gtcttgcttt gagtaataag aacgaagaat
     2701 agaaaacagc tttccatgtt ttctaattct ttgggcttat taccgagaag cagcaggtgg
     2761 aacaccgttt ataacaagag gttgtgacag aagtgggcaa cttcaagaaa taagaaggaa
     2821 tttacgaaaa ttacttttca agtttttgtg aaatttcggc ttatttttcg aagaagctac
     2881 ttctggaaca ccgtttataa caagaggttg taacagaggt tgtgacacct gtgtcttgct
     2941 catggaggct tatctcgggc atcttattcc tttaatttta cataaaaagg aggctgtgac
     3001 ggtttgtctc agcccctttc tattgaaatc ttgcattcaa ataacgttct ttatctattt
     3061 atttcttgcc gtactgggaa cttggtctac cgtaccacca attacctagt gtacgttgta
     3121 gccatggaat tacccagcga tccaacccaa tcgcacgtcc ggaaccgttc atcaatgcga
     3181 aagcaacaaa aatgaaccaa atgtttaccc agtaaaacat gcctgataag cagaacgtga
     3241 tcaccaatac aatcgttgtc gcactgcata gccatgtgaa taatccagca atcaatgcta
     3301 atgccaaggc aatttcaaag aataccatga atttttggaa gaacattgct acatcattgt
     3361 ttggcatgat cacttgcatg atctttgtga accagtctgg tgacttctca atcacagcca
     3421 ttggttcttc accgtatgca taactcaaac caaaatgtgc agcttgagtc gctgcttctc
     3481 cggcacttgc ccctgctccg ctggcagctg tggttgcatc accagcagca gaagcaccac
     3541 tggcaacatc tgttacattg tattcccatg tccaagggaa cacactgttt ccagtgaacc
     3601 atgatccttc accaaaccaa gtacttggtt tgaacacttc accattccca atgatttttt
     3661 tggaagcttc aacgaaccaa actaaaccat agaaaatacg aagtggcaca ctccataaga
     3721 cattgccgta acgagaagaa tgtcctcgag tcacgttgcg atcatctttg atatggaaaa
     3781 actcatgcat taaatattgg aacatataat aaccagaacg aatatcaaag aagtatttca
     3841 aattcacgat atgtttcatt acgatagcta aaaatccgct taaatggatc ttatcaaata
     3901 ggttagctac cccccatttg gcaccaatag aaaccataaa tccttgatag tttcctttaa
     3961 atttatgctt ttctccgcct ttgatcgaag ccacgatatt cgctgcggct gtgtgtcctg
     4021 tttgttcagc agcttgaacg atctgcggtg tcggtgttcc tgggaattct tcataaaaga
     4081 ctaaatcacc aatgatatag atgtttttat cttcgtagcc ttttgcctgc atatattcat
     4141 tggcgatcaa ccgctgtccg cgtgctgatt ctaatccaaa gtcagcagcg tcagaagttc
     4201 ctttgacacc agctgtccaa atcagtgtgt gagtcggaat gttgcgtccg tctttcaatt
     4261 tgatatgatc agcagccact tctacgattg gtgcattcaa taataattcc acgttgtgct
     4321 tacgcaaata gcgttcagct tttgcagcat catttcgatc caacatattc aagatcgtcg
     4381 gcattgcctc tacgaccatc agtgtgattt cgtttggatc taatttataa tctttggcta
     4441 ggcgattttt ccagtcgatc agttccccaa tcatttcaat accggtaaat ccagaaccac
     4501 agacaacgaa agtcagcatg gctttacgca gttcatcatc tggttccaat gcagcttttg
     4561 ctactgtctt ctcaatatgt tcccgaatcc ttacagaatc ttcaaatgac caaagggtga
     4621 atccgtgtgc tttgacacca ggtgtaccaa agtcattcgg ctctccgccc attccaagga
     4681 tcagctgatc aaaaggatag gaacctaatt tggtcttgac gatttttcgt tctttatcga
     4741 taccgattac agtgtctgtg acaagttgta cattcttttg acgagcaaac aaatgctgca
     4801 gatcgtattg gattgcttct ggttctacac gtccgccagc tacttcatgc aactcggtca
     4861 tcatagtatg gtacgaatga cgatcgatca gagtaattgc tacatcttct tttttcagtt
     4921 tctttgccaa gtattttgta gctgaaacac cggcatatcc tgctcctaca acgacaatat
     4981 tttgctttgc cattgatcat ccgctcctta tagactatct atataatgaa tatatgataa
     5041 ttcttttagt ttgatactta ttacacaatc tccatatatt atatagatgt tctgttcttt
     5101 tgtctaactc cttttggatt tttttcaaat tttaaaataa agcgtttttt cacgaaattt
     5161 gctgaaagaa aacgtttgaa atattacatg ggttatttat caaaaatagt tgacttatag
     5221 gctaaaaaga ttagaatgta taatgaatca caaagaaaaa ttgtggacta gaaatggaat
     5281 ggtggggtat taaaatgaag aaaaaacgtt ttgtaactgg atttgccgta ttagcatttt
     5341 cagctctagt gttaggcgct tgcggcgcag atgacaacgg aagctcaaac tcttcttctg
     5401 aatcatctac agcacaatct tcgactgcaa aaagcacaac agaatcatct gcaaaagttg
     5461 tagctggcgg agatttgcaa gacggcacat ataaattaga agaaaaaaat tattcaaatg
     5521 gttaccgtgc agtctttgaa atggttgtaa aagacggcaa aatcactgaa tcaaaatatg
     5581 ataacgtcaa tgaaaacggc gaatcaaaaa caaaagatgc agaatacaac aaaaacatgg
     5641 aagcaaaatc tggcacaaac ccagaaaaat acattcctga gttgaatgac caattcttaa
     5701 aagcacaaag cgcaagcggt gtggaagtcg taactggagc tactcactca tctgaaagct
     5761 tccaaaacta cgcacaacaa ttgatccaag ctgctcaagc tggtaacaca gatacaatcg
     5821 aaatcgacaa tggtgctgat ttgaaagacg gcacttacaa gcttgaagaa aagaactact
     5881 ctaacggcta ccgtgtgcaa ttcgaaatga cagttgctgg cggcaaagta actgaatcaa
     5941 actttgatta tatcgacaaa gacggtaaat caaaacaaga tgatacagaa tataatgaaa
     6001 atatgaaagc aaaaagcggt acagaaccaa aaacttacat ccctactctg aacgacgaat
     6061 tcgtcaaagc aatgggcgaa gaagatggct cacctgctaa tgtagaagtt gttacaggtg
     6121 ctacacacag ctcacattca ttcatcatgt acgcacaaca attagtgaat gcagctgaaa
     6181 aaggcgacac tcaaacaatc gaagtagata atatcgtaac agaaaaataa tcacataatc
     6241 atactaataa aagttggagg gtgggacgat agccagatta ggcagtcgtc tcacctttca
     6301 aaatgaagag aggaaaaaga tgcggaaaaa actactattt tccttaatca cactgggcat
     6361 ctcttttatg gtgctaactg gctgcagcaa ctcagcaaat aaaacaacca ctgaaagttc
     6421 agcagctacc aagatattga aagatccata ttctgatgaa cagttcttgt tagggactta
     6481 cgttcgtatc cgtgtgtatg atgaaggcaa agaatcagca ttgaaacctg ccttcgatcg
     6541 agtaaaagaa cttggtgata aaatcacgat caatcaaaaa ggttctgaga tcgatgaagt
     6601 gaatgagcaa gctgggatca aaccagtaaa agtttctgat gacgtctaca cgttagtcaa
     6661 gcgggcctac gaatatagcc aagattctca aggcggattc gatatggcga ttggtgcaat
     6721 cacacaatta tggcgaatcg gttttgatga cgccagaaaa ccttcccaag aagaaatcga
     6781 tcaggcattg aaattagtgg attatcataa gatcgaacta aatgacaaag aaaaaactgt
     6841 ttatctaaaa gaaaaaggaa tgatcatcga tttaggtgca atcgccaaag gttacattac
     6901 ggacgaagta gtgaaagtct tgaaaaaaca aggagtgacc actgccatcg ttgacttggg
     6961 cggtaatgtc tatgtcttag gccacagccc aagaggcgaa aaccaagact ggacagtcgg
     7021 catccaagat ccgaacatgg ctcgcggatc tgttttaggt tctattaaag aacggaacaa
     7081 aacattagtt acctctggaa tttacgagcg ttatttagaa gtagatggca aaaaatacca
     7141 tcatttgttt gatccaaaaa cgggttatcc gttcgacaat gatatcgcaa gtgtcacgat
     7201 cataacagat aaatcaattg atggtgacgg gttgtctaca gcagtctttt caatgggtgt
     7261 gaaaaaagga ctggaatacg tcgaaagcga attgaataac gggacggaag cgatcttcgt
     7321 gacaaaagac gataaagtat atgtgaccga tccaatcaaa gacaccttca aattatcgga
     7381 ggactcccat tacacgatgg gtgaccgttc tgaactaaaa taaggagtac gaaccatgac
     7441 actaaaaatc tttttagaag tagttgaaat acgaacaaag gtggctagtg tatttccttt
     7501 tgcggtaggg gtattattct ctatcgcttt ttttcatgag atacattggc taaacacttt
     7561 tttatttttt attggaatgc tagtttttga tttagcgact accgctatca ataattacat
     7621 ggattatgaa aaggctcatt ctgaagtata taagtatgaa gaaaatgtca ttggccgcag
     7681 aaatctatct cctgtattga ttcgaaacat gattttagct atgattgcat ttgtttttgt
     7741 tatcggcgtt attctaacga tcaatacggg ttggctttta cttttgatgg gatttatctg
     7801 ttgttttatt ggagtattct atacattcgg accgattcct ttatctcgaa tgcctttagg
     7861 ggaaattttc agtggggtga ctatgggcct tggaattttt gccatggtcg tttatgtcaa
     7921 tacattcggt tcagatgtct atgatctgac agtcaacttt tcatctggac aatttgcttt
     7981 ggtaggaaat ctttgggcta tagcagcaat cgtattagct tccttaccct tggtgtttac
     8041 gattgccaat atcatgttgg ctaataattt gcgggatctt gaccgtgata ttgaaaatca
     8101 tcgctataca ctagtttttt atatcggacg tcctgcggga acagtcttat tccagctatt
     8161 gatgtatgct tgctatttcg tgattttaat ggggttgttg agcggtatct atgaatggcc
     8221 gatcttggtc accttcttga ccttgccaat tgtgaagaag catttggatc aattcaagca
     8281 aacgttaccc cagcctatca gtttcagcta ttccatcaaa aatatggtct tattcaatgc
     8341 cagttatgta ttggggctat tactaacaat cattatgcaa gccatttaac agcatgaaga
     8401 ggttgtgaaa aaactgccct gcatcaagta ataagaacag ccaaataaaa atagctcctt
     8461 atatttttcg tttggttggg cttattaccg cagatgcaag cttttgaaca ccgtttatca
     8521 ggaggttgtg aaaaagtcgc ttagtttcga gtattaagaa aaattttgtg aaaatggctt
     8581 tccacatttt tgcataaatt tggcttaata ccgagaaact ggcttttgaa caccgtttat
     8641 cagaggttgt aaaatcagtg ttttgtcctg agtattagag aaacaaatgg aaaaatagtt
     8701 cctcatattt tgccgttttt tgagctaata ccgaaggaca gctgattgaa cactatttgt
     8761 tcggaaaaag tgactaacta tgacacaatt tttttcacag tccctttttt cacttgattt
     8821 gattcgtacc agcgaacttg acgagaactt tcttggtttg gtaactatat ggtattcttt
     8881 taacatcctc gtttgcttct tctaatttat ttatacaaag tcacactttt ttaaacatct
     8941 gttcttcgat acatttctat ttcttttaaa catcgatacc aatattttga acttaagagt
     9001 gcataccaaa tggattcatt tactcttcgt cagttacgaa gataatcaaa atctccaact
     9061 tccctactta tttcgttttg agaattatcg aagcaatctt acataaataa ctaaaaaaca
     9121 aaaaaccaga aaatcagtat actgattttc tggttttaca tggtttgttc aataaaaagc
     9181 tgttggaact gttaatatta aattatcgca tcgttacgaa ttcttccgct cctgttggat
     9241 ggatggctac tgtattgtca aagtcttctt tagttgcacc catcttgata gccacggcaa
     9301 aaccttgcag catctcatcg acaccaacac caatagcatg taagccgacg attctttctt
     9361 cttttccgac acagatcaat ttcatttcgc atttttggcg ataatcattt aatgcaaagt
     9421 acattggggt aaagcgtgaa cgataaactt tcaattcatt ttcgccatat ttttccaatg
     9481 cttcttcttc tgtcaaacca attgttgcaa ttggtggatg cgtaaagacg actgtcggaa
     9541 caagtttata gtcaagataa gaatctttct ttccattaaa taaacgttct gataaacgtc
     9601 taccagctgc gattgcaaca ggagtcagat ctaatttacc gatcacatca ccaacagcaa
     9661 aaatatgttc atccgttgta ttttggtatt tatctacttt gataaaccct tttttgtcca
     9721 attccacatt tgtattttcc agacctaatt tttcggtatt cggtacacgt ccaccggcaa
     9781 aaagtacaca atcaccagtt aaggtttcac cattttcaaa tagcaccgta tattcgtcat
     9841 tgtttttagt aatttcttta gcaatatgat gtgcgtaagt ttttattcca gcttcttggt
     9901 acatttggat caaattgtcc gaaagcattt tatcaaacgt tctaagtggt cgttcttttc
     9961 gatatgccca catgacttca ctacctaaac cacaaaatac acctgaaagt tcagccgcaa
    10021 tgtatccagc acctaaaacg attgctctct taggtaactc tgttaattca aaaaatccat
    10081 ttgaatcaat tgcgtattct ccaccaggaa tggtcatttt acttgcacgg cctccagtag
    10141 cgatcaagat tttaggagca cgataggttt caccgtttac ttcgatcgtt tgactatcga
    10201 taaaagtagc gtaacctttt atagcctcga catgattgct atctaatcct ctttgataag
    10261 caccgtgtag aaaatcaata tatttttctc gtctttctac tagaccggcg aaatcaacat
    10321 tttttaaatc tgcttggata ccgtaacttg aggcatctcg ttcaattgtt tccaaaatcg
    10381 tacttgcttg ccacataact tttttaggta cacaaccgac gttgacacat gttccgccca
    10441 attcattcgc ttcaatcagc aaaacttttg cgccatgcat ccctgctctg tttgctgaag
    10501 caataccgcc agagccgcca ccaattacga tgtaatcata tatttgcatt ttgttcctcc
    10561 ttattgaaga gatcaatcaa aacctttatt taacgatcct ttttctacag aaaacaagtg
    10621 taacactttt cttgaatttt gtatgcgaag atgcttaaaa cagggatata tcagcttttt
    10681 ccttatttac gttttcggat catatctcca ggtttcaccg gctgtgcgac tggcatagtt
    10741 aaaatcatca ttggatttgg tgctcggtca atggattcgc cttcttcatt atgcataact
    10801 tcaacggttt gactgaaatg actgaatcct ggaccataaa attcgatttc atctcctaca
    10861 gaaaacaagt ttctctgacg tatcgtagca atctgtgttg cctcatcata agataatact
    10921 tcaccgacaa atttatatac tgggatttta cgacgttcac cgaataattg ttcgttttca
    10981 gttggtgtat tgtagtaaaa acctgtagct aattctcgtt gtgctacttt ccataactcg
    11041 tcgatccatt cttgcttgca gacatagttt tcaggatctt ccatataact gtctacagcc
    11101 gctttataga catttgacac cgtagataca taatggatcg atttcattct tccttcgatc
    11161 ttgaagctgt ctacgccgtt ttcaatcaat tctggaatat gttcgatcat tgacatatcc
    11221 actgcactca tcgagaattc ttcttccact tcgcctgtat cagttaaact gtttcgttct
    11281 tgaccaaaag gcatatcgaa caatccatac ttccagcgac aagattgaga acatccgcca
    11341 cggtttgcat cccgcatcga catatggttg gacaatgtgc atcgaccaga ataagagata
    11401 cacatggcac catggataaa tgcttcgatt tcggtatccg tatttttacg aatctctgcc
    11461 acttcctcca ttgaaacttc acgagctaaa acgacacgtt ccaacccttc ttctttccag
    11521 aattccaatg tctcaaaatt agttgctgaa gcttgagtgg ataaatggat cggcagacct
    11581 ggggcttctg tcgcacaaat ttcgatcaaa gctggatcag aaacaatcac tgcagaaata
    11641 ccgacatcac gcaaactgcg gaaaaattcc cctgctccat cttcatttcc ttcatgggtg
    11701 accatattag cagcaacgta taccttcgca tcatgttctt ttgcaaaagc aactccttca
    11761 gccatttctt cataagtaaa attacctgca cggcttctta aaccatatgc attcccgcca
    11821 atatatactg catctgctcc ataatggatc gcggttttta acttttccag tgttcctgct
    11881 ggcgctagga tttctggtcg tttcaatttt ctttttactg tcatttcttt catcatactc
    11941 cttatttgat agcagaagga tcaatataaa agaatcctgt atctaattca cggttttcag
    12001 gatgcaattt actgatttcg ctagtcattt tttccgcttt ttcttgatcc cagctacctt
    12061 ctaaaatttg tttttttgct tcggcgaata gtcgagtgat gtttacgaat ttttcgcctg
    12121 gagtaaataa tccgtccaat ttccatgtac agtaatgatg ctgataaagt tcattcaatt
    12181 cattcgagag attcagatca ttgctggcaa aaatatgtgt gccatgacta tcttcataaa
    12241 tagaataatg tgtctcttct tttttaggtt ccgagataaa aagacctctt tcacgatctt
    12301 tttgttcgtc ttgtttcgta tagttatagt aattctgtag cagtggacgt ttggattgat
    12361 ggatgcatgt agcaccatac acaagggttt ctacaggaat atcgagtttt tcttccatcg
    12421 ccttcatctc ttcaaaagga acttcacgag caagtactgc tccaactgcg ccttttttcg
    12481 cccagaaatt gatttgacgt gcgctcgtta ctaatgtttc agcatcataa atatacggta
    12541 tacgcaattc aggatttttt ttcatcgtat aaataatacc tgtatcacca actgtgatca
    12601 catctacgtt tatttctttt aaaaaagcca aatattcagg tacatttttc attttttcag
    12661 gatgaatgat cccatttaca gcaatattcg cttttttacc atacttgtgt gctaattcta
    12721 ccaattgtcg ctgttcttct cgtgtaaatg aagcaggcag acgcaatcca aattgctctt
    12781 caccgaaata tagtgtatct gcacctgcag caagcaactg ttctgcttgt tcaatagttt
    12841 ctactgtggt tatgagttcg atcataattt tctcctccta caacttcata atagtagcat
    12901 agctctaaaa actttccaat acttcttcta tttaaagtcg gatttttgaa aattatttca
    12961 caaaaaatca aaataaagtt cgatatattc tgtccaaata taaaaagaga ttgtgacaaa
    13021 aatcttgtca caacctctta atcaagtctt actattataa aaattcagct acacaggcca
    13081 agtaactata gggaacctac tgtttccttt ttaggtccgc gaacattcaa tgtgatctta
    13141 ccttttgaag cacctactgt tacgtggtca cctaaacgaa tttctccgcc aagcaaagcc
    13201 tcgctcaatc gatcttcaat ttctttttga agtgcgcgac ggattggacg tgcaccatat
    13261 tcaggatcaa atccagcttt cccgatcaca tcgattgcag atggtgtgat tttcacttga
    13321 atatcttgtt ctttcaatcg tttgataatc gctttgctca tgatttggac gattgtatgg
    13381 atttcatctt tactcaatga atggaagacg acagtctcat cgattcgatt caagaattca
    13441 ggtcggaaag ctttctttaa ctcttctaag atacgttttt gcatggcttt gtgatcttta
    13501 gtaacatctt ggacattgaa tccgacattt ttttcttctc gaatttgtgt cgctccgata
    13561 tttgaagtca taatcatgat tgtatttcta aagtccactt ttctaccttt agaatctgtt
    13621 aaatgaccat catccaatac ttgcagaagt aaattgaaaa cgtctggatg tgctttctcc
    13681 acttcatcta agagaatcac tgaatatggt ttttgacgaa ttttttctgt caactgtcca
    13741 ccttcatcat accctacgta tcctggagga gacccgatta atcggcttgt actgtatttt
    13801 tccataaatt ctgacatatc aacacggatc agggcatctt cacttccaaa catgacttct
    13861 gaaagtgctt tggccaattc tgtttttccg acaccagttg gtccaaggaa catgaatgaa
    13921 ccaattggac gatcaggatc tttcaatccg cttcgtgcac ggcggatcgc acgtgaaact
    13981 gctttgacag cctcatcttg accaacgaca cgttcatgca gaagtccttc cagttctaac
    14041 aagcgttcgc tttctttttt ctccaactgc tgtagaggga caccagtcca ctccgataca
    14101 actgtagcaa catcttcttc tgttacacga tcagaataac cttgctcctg ttttgcttca
    14161 attgcagaaa ctttttgcag ctttttacgt agtgcttttt cttgtctgcg taattgagca
    14221 gcattttcaa agtcttgttt ttggatcgct gcttcctttt cttgtactag ttcatcgatt
    14281 tctagtttga tcacagttga ttttgttaag tgatcggttt gatccaaacg gactttcgct
    14341 gctgattcat cgatcaaatc aattgcttta tcgggtagtt gacgagaagt gatataacga
    14401 acagaaagat tgactgctgc tcgtacagct tcatctgtga tctccactcc atggtgctct
    14461 tcatatcttg tacgtaatcc ttgaagaatc acttctgctt cttcaggagt tggctcatct
    14521 acttgaatgc gagcaaaacg tcgctctaat gcagaatctt tttcgatata tttttgatat
    14581 tcatctaggg tcgttgctcc gatcgtttgt aattctcctc gagctaacgc cggtttcaag
    14641 atattcgaag catcaattgc tccttctgct ccacctgcac caattaaggt atgcaattca
    14701 tcaatgaata agattacttg accatcatta tagatttcgt cgatgacctt tttcattcga
    14761 tcttcaaatt caccgcgata tttcgtacca gcaaccaaag atcccatatc aagcatcatc
    14821 aggcgctttt cttgcatgtc ttctggcact tcacctaaaa tgattttttg agctaaccct
    14881 tcagcaatgg ctgttttgcc tacaccaggt tcacccacca gtacagggtt attctttgtc
    14941 cgtcggctaa gaatctggat aagccgtttc acttctctcg aacgaccaac aactgggtcc
    15001 agcttgtttt cttttgctaa tttcgtcaaa tcacgtgcta atgaatctag cgtaggtgtt
    15061 ccttgcggtg tttgccgttt atcttgtaca tttacatttc tacgacgatt cattcctttt
    15121 ccagcagaat caccagctag ccccattttt tgtttcagaa cttgacgggc ttttgaaagg
    15181 ctgataccta agttgatcat gattcgagta gccatgatac tttcatcttt taataagctc
    15241 atcaaaatat gttctgttcc aactttttga gaaccagttt tcttcacttc gctgctagct
    15301 aaagcaaaaa cttgtttcat tcttggtgaa tatggaagaa agactccttt agggtaagaa
    15361 cggataccac cataaccaat cagatgttca atttcttcat aaatttccgt ttcattcaaa
    15421 tccatttcac gtaatacttt tccagcaatt ccattttgct cgaccactaa ggctagtaaa
    15481 acatgttctg aactaactga ttgatgtttc aaccgttttg cttcttcttg tgcaatttct
    15541 aatactgctt gggctctttc agtaaaaagt tcttccatct aatctcactt cccatcttca
    15601 taacttaaac gttccagaaa tgctcggaga atccgagcac gtgtatgatc ttcatattca
    15661 ttgaaattca atgtgttttt tgcaattgca ctcaacatca agtttccttc tttttttgta
    15721 attatttggt cttcataaag tttttgaata atagcaaaag catttttctc actgattgta
    15781 tcatcaaata actgatttat ctgctcaagt aattgttttt tatctgaaat ccttactttt
    15841 gcaattcgta tataaccacc accaccacgt ttgctttcta ctagataacc acgttggatg
    15901 gtaaaacggg tattaatcac atagttgatt tgtgaaggaa cacagttaaa gaggtctgcc
    15961 atttcaatcc ttcgaatctc gatcatttca ctattttcca gaattttctt tatatatgat
    16021 tcaattaaat ccgaggtatt ctgatgtgcc atcttattca ttccctttga ctaatattga
    16081 ccttaattat aacgaatttg ataatagaaa gaaagaagaa cgctttttgt taaataaatc
    16141 ctttagaatc aacaaaaaaa aatacggaat atactattcc gtatc
//
LOCUS       DAFGZE010000063        15899 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.065, whole genome shotgun sequence.
ACCESSION   DAFGZE010000063 DAFGZE010000000
VERSION     DAFGZE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15899
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.065"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(52..522)
                     /locus_tag="LTU54_002164"
     CDS             complement(52..522)
                     /locus_tag="LTU54_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HBL8296866.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="LTU54_002165"
     CDS             complement(681..1139)
                     /locus_tag="LTU54_002165"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBL8296867.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2454)
                     /locus_tag="LTU54_002166"
                     /pseudo
     CDS             complement(1310..2454)
                     /locus_tag="LTU54_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
     gene            complement(2467..3384)
                     /locus_tag="LTU54_002167"
     CDS             complement(2467..3384)
                     /locus_tag="LTU54_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBL8296868.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3812..4375
                     /gene="efp"
                     /locus_tag="LTU54_002168"
     CDS             3812..4375
                     /gene="efp"
                     /locus_tag="LTU54_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBL8296869.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4476..4646
                     /locus_tag="LTU54_002169"
     CDS             4476..4646
                     /locus_tag="LTU54_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296870.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            4861..6120
                     /gene="lysA"
                     /locus_tag="LTU54_002170"
     CDS             4861..6120
                     /gene="lysA"
                     /locus_tag="LTU54_002170"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBL8296871.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6107..6907
                     /gene="dapF"
                     /locus_tag="LTU54_002171"
     CDS             6107..6907
                     /gene="dapF"
                     /locus_tag="LTU54_002171"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBL8296872.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILGE"
     gene            6910..7791
                     /locus_tag="LTU54_002172"
     CDS             6910..7791
                     /locus_tag="LTU54_002172"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBL8296873.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7788..8504
                     /locus_tag="LTU54_002173"
     CDS             7788..8504
                     /locus_tag="LTU54_002173"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBL8296874.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8510..9205
                     /gene="dapD"
                     /locus_tag="LTU54_002174"
     CDS             8510..9205
                     /gene="dapD"
                     /locus_tag="LTU54_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBL8296875.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9221..10222
                     /locus_tag="LTU54_002175"
     CDS             9221..10222
                     /locus_tag="LTU54_002175"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBL8296876.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10219..11580
                     /locus_tag="LTU54_002176"
     CDS             10219..11580
                     /locus_tag="LTU54_002176"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBL8296877.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11540..12691
                     /locus_tag="LTU54_002177"
     CDS             11540..12691
                     /locus_tag="LTU54_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBL8296878.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12666..13835
                     /locus_tag="LTU54_002178"
     CDS             12666..13835
                     /locus_tag="LTU54_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL8296879.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14010..15389
                     /locus_tag="LTU54_002179"
     CDS             14010..15389
                     /locus_tag="LTU54_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL8296880.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            15670..15899
                     /locus_tag="LTU54_002180"
                     /pseudo
     CDS             15670..15899
                     /locus_tag="LTU54_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5200 a         2803 c         3385 g         4511 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatctttt ggttagaagt cactaaacca gctactacat cgctgtgtcc
     1861 gcctagatat ttggtaccgc tatggacaac gatatcagaa ccgagatcta aaggtctttg
     1921 aaaataagga gtagcaaatg tattgtcagc aataactatc aatccatgtt gatgagccag
     1981 agtggcactt ttttctaaat cagtaatctt tagaagcgga ttgctaggtg tttcaagata
     2041 aagtgctttt gtattgggtt tgatggattg ttctatttta tcaagattac tcgtatctat
     2101 gatcgtgtac tcgagaccat ttttagtaag aactttgtca aatagacgga atgttcctcc
     2161 atagacatca tctcctaata agatatgatc gccagcttga aaaagagaaa aaacggcatg
     2221 aatcccagaa agaccagaag aaaaagcgaa tcctcgaaca ccgccttcca aatcagcaat
     2281 cagttcttcc aatgcaaaac gagtaggatt accagatctt gaatattcat attgtttagg
     2341 ctggccgacg ccatcttgcc gataagtgga tgtttgataa ataggtacac tgacagctcc
     2401 tgttgtggga tcttcactga tgcctccgtg gatcaatttt gtttgaatgt gcatgtttct
     2461 ttcctcctat aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt
     2521 aacgacacga tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc
     2581 accactcgaa cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa
     2641 cccttctaca tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg
     2701 aatgaattct acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac
     2761 agagccctct ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt
     2821 agaagttcca gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc
     2881 tttgatttca tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt
     2941 attttcaaat tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt
     3001 gctgatggca ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc
     3061 tttcatcaat tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa
     3121 tttgtatgtg agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat
     3181 gatcgtcgtt ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc
     3241 caatctatct ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga
     3301 acctttaggc atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc
     3361 ttctgtaatt gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc
     3421 tgactattaa atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag
     3481 ttacctggta ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt
     3541 tatgcaccat acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc
     3601 ctccaagtaa aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa
     3661 gaaaaaaata tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga
     3721 gaccttaaaa gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg
     3781 attatgataa agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg
     3841 tatgactttc gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc
     3901 tggtaaagga aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata
     3961 cgacacaacg ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt
     4021 acaatatttg tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca
     4081 atatgaaatt cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga
     4141 agtaaaaatc caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt
     4201 attacgtgtt gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa
     4261 gccagcgaca atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga
     4321 attgttggaa atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc
     4381 taacggattg ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat
     4441 ctgcctttca agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt
     4501 ttatttatat tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt
     4561 atgaagaaat tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg
     4621 aagcgaaaat attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag
     4681 cttctaatag ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat
     4741 atttcgaaat atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca
     4801 aatctttatc aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa
     4861 atgacagaat tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca
     4921 cctctgtatg tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc
     4981 tttgtatcga aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt
     5041 gagatgctgc atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa
     5101 atctataccg ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac
     5161 aagacgccag aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat
     5221 aatttgatgg aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt
     5281 atcatgcttc ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg
     5341 catattgatt caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag
     5401 ttgataaaag aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa
     5461 atttttgatg ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat
     5521 tttgaccaac ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat
     5581 gatcagccga tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct
     5641 ttggaaaaac gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt
     5701 ggagaagctg gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa
     5761 tattactttg tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa
     5821 tacgattgtg atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca
     5881 ggaaaaatgt gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa
     5941 tcgggtgatc ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac
     6001 tacaatcgag cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt
     6061 gttcgcagac aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa
     6121 atataatggc tgcggaaatg attttatctt attagattac gcagaagaca cagattatag
     6181 tcaattagcc gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt
     6241 aaaaatcgat ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg
     6301 cggaaatggc attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca
     6361 ggaattcaac gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc
     6421 tttttattgc agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa
     6481 tcaaacttat ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac
     6541 tagtttattt ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt
     6601 ctggagaaaa agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt
     6661 caactttgta gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt
     6721 aggttggaca cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac
     6781 tgggaaaatt tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc
     6841 tggtgaggat gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattggg
     6901 ggaatgaaga tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga
     6961 tcagtagatt atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat
     7021 ggattattag ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag
     7081 cagatccttc aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct
     7141 ggaacgaata atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca
     7201 gacgcacttt tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct
     7261 cattttacag caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct
     7321 agaacaggca tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc
     7381 attggtttga aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt
     7441 ggagaagaat tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta
     7501 ggaggaagcg gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta
     7561 gtaaaagata cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt
     7621 gaactgatcc atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca
     7681 ttattagact taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa
     7741 aagaaacaat tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac
     7801 tgattggcag tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata
     7861 cggtccttct tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc
     7921 cagaactgct tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct
     7981 tatcatatca acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag
     8041 aaataaaaga acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca
     8101 tcatggtgat gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg
     8161 aactgattga aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc
     8221 tgcttcaaac aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa
     8281 aaagagaaga aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac
     8341 atgatgtatt gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta
     8401 atcgaatctt tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag
     8461 gttattacaa attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca
     8521 agaaatcatc cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa
     8581 tgtaaccgag ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt
     8641 tgtttttggt gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat
     8701 tgaaatcgaa atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa
     8761 aattaatgct cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa
     8821 tgccgtgatc atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat
     8881 gattgatatg ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg
     8941 tgcaggagct gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga
     9001 agacggcgta ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga
     9061 tgcagtcgtg gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg
     9121 tggaatacct gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt
     9181 gattgctgcg ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg
     9241 tattgtgggg gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga
     9301 ttttccagta ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt
     9361 attcaagaaa aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga
     9421 tttagcgcta ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag
     9481 tcagggacta tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt
     9541 aatcgttcca gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa
     9601 ttgttcaacc attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca
     9661 gcgagtcaat tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga
     9721 tcttcatcgt acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac
     9781 ggtgattccg gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat
     9841 gataaatgag acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt
     9901 acgtgtgcca atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt
     9961 tacgacggaa gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga
    10021 tctagcagca cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt
    10081 tgggaggatc agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga
    10141 taacatacgc aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa
    10201 aatagcggag gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg
    10261 atgtctcatg aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc
    10321 caagtgatcg ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac
    10381 ttactttatt tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa
    10441 aggatttgtg atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa
    10501 gatgaactag cacgcataca gaaacaactg gaagacgaaa agataactga agattattta
    10561 gtaagtagag gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt
    10621 attgacgcaa aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca
    10681 gcacaggaaa tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac
    10741 ggggcgaacc ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc
    10801 gcgatactgg cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga
    10861 atcatgatgg ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt
    10921 agagaactga gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc
    10981 ccagttcaag aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa
    11041 gggacaaaaa tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga
    11101 cgcaaggatt ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt
    11161 atctggaaag caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca
    11221 gggatcgaca atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc
    11281 atcacgaaag aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta
    11341 gctttgatct ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg
    11401 ttatcgatat tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag
    11461 ttgaatctga tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat
    11521 gaagggatgg tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga
    11581 aatcgggttt catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga
    11641 ggccgttatc catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga
    11701 agaaacgatt gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct
    11761 ctcttttgct tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc
    11821 gatgctttta gggttgactg actttataac taaacacatt gaggatctta tttataatgt
    11881 agtactgatc tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc
    11941 aggattgttg gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact
    12001 agctaaggga gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga
    12061 cattatcatt aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct
    12121 ggaggcagct gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga
    12181 gtggaatcat ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc
    12241 tggagaagtt attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt
    12301 gaagagggga ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg
    12361 ctataatgac ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga
    12421 ttcaaacaaa ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata
    12481 tacgcaacaa tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca
    12541 tgatccgacg ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac
    12601 gattcttttt acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt
    12661 aaataatgat gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa
    12721 aaatacgaca attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg
    12781 gagaaccgga ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag
    12841 aagacttttc tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta
    12901 tatttcagca acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag
    12961 ttggtgcaac ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg
    13021 ttcttattcc agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg
    13081 aattgatccc gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac
    13141 gagcatttga acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc
    13201 ctactgggac gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat
    13261 atccggtatt tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata
    13321 tgtccatagc tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac
    13381 atgcgatgac tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac
    13441 taattaaagt ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg
    13501 ctgaagcgct aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac
    13561 gaagagacta tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg
    13621 gtgcatttta cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt
    13681 gtgtagaatt agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag
    13741 aaggagaagg ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct
    13801 gcaaacggat caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa
    13861 tcgttaattt tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg
    13921 aacgcgtttc ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt
    13981 ttaaaagaat attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg
    14041 catctcatgg agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag
    14101 atatcacgct atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt
    14161 atttagaaga caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc
    14221 cgcaatccaa tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa
    14281 aaacagtaac ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat
    14341 tgattttgag ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa
    14401 atgtctactt gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc
    14461 cggctattaa aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag
    14521 caagccgaaa agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca
    14581 cttatttaga tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg
    14641 aagttttgac aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca
    14701 aaacaagtga aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa
    14761 atacagaatg gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg
    14821 aatacttgca aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt
    14881 ctatcccagc acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac
    14941 gttatgtagt aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag
    15001 gttcctctgc attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta
    15061 cagcagcaaa agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa
    15121 aatatgtacc agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa
    15181 tgacccatca attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa
    15241 atgtattggc actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact
    15301 tcttcttcca gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag
    15361 cgttaggaga agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa
    15421 aaagctgcct tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta
    15481 acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag
    15541 ggactgtgac acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg
    15601 gaggtcggta ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga
    15661 ttatttggat ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa
    15721 atgcttaaaa ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta
    15781 agaaaccgcg gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga
    15841 ataaaagtaa cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DAFGZE010000064        15714 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.066, whole genome shotgun sequence.
ACCESSION   DAFGZE010000064 DAFGZE010000000
VERSION     DAFGZE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.066"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            12..335
                     /locus_tag="LTU54_002181"
     CDS             12..335
                     /locus_tag="LTU54_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010714055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296881.1"
                     /translation="MSLTVRVLVQKVGDLSDYYNCKREVIVDNRYDQSKDDKRIIKIG
                     DIVFPEKGIAYEVVSINSFRYEFVDLVVEYSVRLIVPWSKEEINEALKDERKSTFKVL
                     EGEKK"
     gene            332..811
                     /locus_tag="LTU54_002182"
     CDS             332..811
                     /locus_tag="LTU54_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296882.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGRERVDYITYS
                     VDNTVRCYEIKVTLSDLKSKAKQTFIGDYNYLVVTSDLWEKMLQDDTLMRKYYKQGVY
                     VFNEKYPSMISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIVEIPE"
     gene            950..1489
                     /locus_tag="LTU54_002183"
     CDS             950..1489
                     /locus_tag="LTU54_002183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296883.1"
                     /translation="MNKRRILWLIAGMYLCIAVSGFTICRIINQLKNAIVYLGEIGYL
                     EDLKAGLQIMSWILFVLTVALVILTCENFSTIKKQIISNHNQETVKSGLEVDPEEEVT
                     EQAIKEQEEIDKKEIMSIYLSSGESFFHEIDSLKLSSQEIIDRWQNMSLDGELIVEDS
                     EGWSAYKKDEIVAITILFV"
     gene            2140..2193
                     /locus_tag="LTU54_002184"
                     /pseudo
     CDS             2140..2193
                     /locus_tag="LTU54_002184"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2197..2796
                     /locus_tag="LTU54_002185"
     CDS             2197..2796
                     /locus_tag="LTU54_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBL8296884.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIHFDSKEHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            2812..3288
                     /locus_tag="LTU54_002186"
     CDS             2812..3288
                     /locus_tag="LTU54_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296885.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMHE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            3305..4207
                     /locus_tag="LTU54_002187"
     CDS             3305..4207
                     /locus_tag="LTU54_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296886.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            4280..4471
                     /locus_tag="LTU54_002188"
     CDS             4280..4471
                     /locus_tag="LTU54_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296887.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            4636..5223
                     /locus_tag="LTU54_002189"
     CDS             4636..5223
                     /locus_tag="LTU54_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296888.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            5223..6122
                     /locus_tag="LTU54_002190"
     CDS             5223..6122
                     /locus_tag="LTU54_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBL8296889.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            6122..7036
                     /locus_tag="LTU54_002191"
     CDS             6122..7036
                     /locus_tag="LTU54_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296890.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            7088..7303
                     /locus_tag="LTU54_002192"
     CDS             7088..7303
                     /locus_tag="LTU54_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296891.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKKFWLNNFSYKDFIKEKANTLY"
     gene            7493..7747
                     /locus_tag="LTU54_002193"
     CDS             7493..7747
                     /locus_tag="LTU54_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296892.1"
                     /translation="MKKEELIDIFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLNSDDVNFAHDVVGIQNHIDRENKRMGDEFLPRYSSL"
     gene            7926..8969
                     /locus_tag="LTU54_002194"
     CDS             7926..8969
                     /locus_tag="LTU54_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HBL8296893.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            9142..9366
                     /locus_tag="LTU54_002195"
     CDS             9142..9366
                     /locus_tag="LTU54_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296894.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            9369..9626
                     /locus_tag="LTU54_002196"
     CDS             9369..9626
                     /locus_tag="LTU54_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296895.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            9728..10081
                     /locus_tag="LTU54_002197"
     CDS             9728..10081
                     /locus_tag="LTU54_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296896.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            10134..10928
                     /locus_tag="LTU54_002198"
     CDS             10134..10928
                     /locus_tag="LTU54_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296897.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            10957..12075
                     /locus_tag="LTU54_002199"
     CDS             10957..12075
                     /locus_tag="LTU54_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296898.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGNSKDEVKGVVIDDDFEFDFD"
     gene            12108..12428
                     /locus_tag="LTU54_002200"
     CDS             12108..12428
                     /locus_tag="LTU54_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296899.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            12683..13576
                     /locus_tag="LTU54_002201"
     CDS             12683..13576
                     /locus_tag="LTU54_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296900.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EDEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHEELSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNAYI
                     ARLKRFIDKYKENSDKFSKDK"
     gene            13596..15224
                     /locus_tag="LTU54_002202"
     CDS             13596..15224
                     /locus_tag="LTU54_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296901.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKEFEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            15275..15643
                     /locus_tag="LTU54_002203"
                     /pseudo
     CDS             15275..15643
                     /locus_tag="LTU54_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         6032 a         1944 c         3220 g         4518 t
ORIGIN      
        1 ttagcaaatc tattagttta acagttagag ttctagttca gaaagtcgga gacttgagtg
       61 attattataa ctgcaaaaga gaagttattg tggataatcg atacgaccaa tcaaaagatg
      121 ataagcgaat aataaaaata ggagatattg tctttcctga gaaagggata gcatatgaag
      181 tagtgtctat caatagtttt cgctatgaat ttgttgattt ggttgtcgaa tattctgttc
      241 gattgatcgt tccgtggagt aaagaagaaa ttaatgaagc attaaaagat gaacgaaaaa
      301 gtacattcaa agttttagaa ggtgaaaaaa agtgaaaaca gaccttacta gacaagctga
      361 gaagtgttta tggcatgaaa caaatcgcat gggtgtattt ggttgttttg aagtaactat
      421 cggttgggat ggcagagaaa gagtagatta tataacttat tcagtagata acacagttcg
      481 atgttatgag atcaaagtta ctttatccga tttgaaaagc aaggcgaaac agacatttat
      541 cggcgattat aactatttag tcgttactag tgatttgtgg gagaaaatgc tacaagacga
      601 cacactcatg cgtaaatatt ataaacaagg cgtatacgtg tttaatgaaa aatatcctag
      661 catgataagt gttaagaaag caaggaaaca aaaagtttcg ctaggtacaa gatcaactgt
      721 attagaaagc atggttagat cgatgaatcg tgaagttaag aaattatata aagtaaaacc
      781 attttgggag atagtagaga taccggaatg atgagtaagg aatattatat ctattcatat
      841 atagtattaa ctgattatga ggaggataca gcaaatgaaa aattatgatc cgaatatgcg
      901 atggggtaca catactctta aagtgtcttt tcaacggagg ataagctaaa tgaataaaag
      961 aagaatactg tggcttattg caggaatgta tctttgcata gcagtatctg ggtttactat
     1021 ttgtcggata ataaatcagt taaaaaacgc gattgtttat ctaggagaaa ttggttatct
     1081 agaagattta aaggcaggac tacaaataat gagttggatt ttattcgttc tgacagttgc
     1141 gttagttata ttaacttgtg aaaatttttc aacaataaaa aaacaaataa tatctaacca
     1201 taatcaagag actgttaaat caggtctaga agtagaccca gaagaggaag taactgaaca
     1261 agcgatcaaa gagcaggaag aaatagacaa aaaagaaata atgagtattt atttatctag
     1321 tggagagtca ttttttcatg aaatagattc gctgaaactt agtagtcaag aaataattga
     1381 tagatggcaa aatatgtctt tagatgggga gttaatcgtt gaggatagtg aaggttggtc
     1441 agcttataag aaggacgaga ttgtagctat cacgattttg tttgtatagt aatatatacc
     1501 actagataat ctgaatatgt tgatatgcta gtgacgtacc aaatgtgttc aatagagccg
     1561 tggtcacagg cagaaattga taaaatagta aaatttaacc gattatcaaa atttaacgtt
     1621 ttaggaaata ccttttaaaa atcacaatac acaaacagaa aaataccaaa aaattaacaa
     1681 tgaacgtagg taagaacatg acaataaatg ataataacct caaaaaatat aatgctcttg
     1741 tccaacgcta tatcgaaaac aaaataacga tgcaagaggt cgctgatgaa ttaggcaaat
     1801 caaaaagcct agtatcacac gactttaagc acaatgtttt tccgaacccg attgagctga
     1861 ggaaaaaatt tcaaaagaga gtttatcaag aaatagggaa gtggattgat gaagggaatg
     1921 caattgaccc tttaattgaa aaatatcctt ttttaaaaag caaaaataaa attcaaaggt
     1981 taattcgtca aggcgatatt caagctaaag aacctgtttt gacaaccaat gatttggcta
     2041 aaataaatct tcataaacaa attattgaag actttgaaaa aaatctttca acaactgaaa
     2101 ttttaaaaaa atacaatatt ccgagaggaa agctaacgtc gactcttttt agagaatatg
     2161 gagaatcggc agttactaaa gcaaaggaag tagcgaatga gagaaaaaaa caaaaatagt
     2221 agtcgatact tagtagcgac tgtagttttt gtttgtgtat tgattttcct gtttgatttt
     2281 ttacagattg gaaaaagtat aattggaaat acattgagtg caaaggaaat ggttgcttta
     2341 ggtggtttgt atgaaggaca tttgttcccg aatatattca tttctatcgt ttctcatatt
     2401 aacttgtacc atttaatttc taatttattg ttcttagtag ttcttgaaga agcactgaat
     2461 gatcagtatg agtggttaac attattgatc gttttaatca tatcaggatt tctaggaagc
     2521 ttagttcctt ttctgctaca aagcaacatc gtaagtatgg ggcttagtgg aggtattttt
     2581 ggatggattg ctttatttgc gattgcatac atccattttg atagcaagga acatttatta
     2641 ctaagtgttt ttgtattatt gataatcggc attttattgg tatctacttt tgaatcaggc
     2701 gaggcaaatg tcttgtcaca tgtaatcgga tttgtctgtg gaatgatctt gggatttatt
     2761 ctaaagaacg tttcattgat caaacaaaag aattaaaagg gagtgctaaa aatgccaaaa
     2821 agaaaagaaa agaaaattcc agtgtttttg tctgtctcag aaatgttatc acttcaaata
     2881 ctgttaagaa aaaagattga aacaatagac gaagaagtta gaaataaaag ggctatgcac
     2941 gaacaacgtg aagcgctaag agaacgaatc cactattttg atgatataca cactaaaatc
     3001 tatgaagcac gtacaaatca cgaagacgaa agtaaattag gattagctca acaacggata
     3061 cttgaatggc ttaatacgaa tactgatcaa gaaccgttaa gagtgattag taatcttttt
     3121 gaatttggaa cgaagcattg gtttaatgct gtagagaagg atgctgactt acaagaagcg
     3181 tttgatacgc taagtaagga tagcaaatcc atgcgagatt tattgattac ttatctaaat
     3241 gagcatgagt ttgaacaaac gaaaaccaaa aagaaaaata aagaatagaa agttgtgatg
     3301 aatcatgcta ttaaccctgt taggttattt atgtttaatt cttgcttttt ttggggcaat
     3361 cgctataatt attggtcaat ttcaaacaaa taatccgatt atgcgtaaca taggcattgg
     3421 aattgtgatt gtaaccttta ttgtgtttaa tgccactaag atttttggaa gtaacgataa
     3481 acaatttggt atatcgggag ttaataatag ttatgctcca gattctttgg gaagggtagt
     3541 aatagatgga acatttgaaa atgtaacgag ttataaaaat gtttcgctga cagtgaagga
     3601 agatggcaag aaagcatcag acgacgttct cgctactatt aagccgtctc aatcacaatt
     3661 tgaagtgttt tatacagtta aagagaatag ccctgtacac aagctgacat tggtttttaa
     3721 cgttgatgga gaagaatact ataaagatgt aaaaataaat aaaacatcaa cgaaaaagat
     3781 tatcgaatca gcgaaaaaag aagtagctac agaagaaagt acaactgaat caagttctac
     3841 tgtcgaagcg gaactaccag ataaaattga ttttgatcct gaaaaagtca aatcgtttga
     3901 atctgtttct ggttctatgg ctgatcggtt taacactgaa gtcttcaaag ctatcaaaac
     3961 tgttaaattg gatactgaaa agcaaatgat taatgttacc ttattcaatt cagttgaatc
     4021 tatggatcag gataaaaaag ttacggttgc aagagaacta ggaaaaagag taattggttt
     4081 gtatcgtggt actgctaaac cagattcaga cggtgcagat ttgccagttc atgtagttta
     4141 tgaagacggt aaagtttttg gagattctac agcaaacgaa gcattaacga ttaaattggc
     4201 taaatagcca gaaagttata ataaacaaat ggtgtcacac caataatata taggtgtgac
     4261 accttggaga tgatagctga tggtagtaaa taaagaaaaa gttagaaagt atgtgacttt
     4321 aaaaatagct tctgttaaat atttagaaaa gcgagctaaa gaagaagaaa gagcaagagg
     4381 taagaaagta acagtttcag agttgttaga agaattgatc gagaatgatc aaacaataag
     4441 acgtgcattt actaataggt ctatcagata acaatataat taataaaaaa agaaagagtg
     4501 atttggtctt agactaaatc actcttttta tataaagttt ctttacactc attccgaata
     4561 atgttaaaat tctttatacg atatatagtt ccttgtcaga ggaagagata accagcaata
     4621 ttggagggat aataagtgga aaacaataat gatattagac attttagcat tggggaaaga
     4681 gttacaacag taagtgaatt atcaaaggat tatagagata aagagggaac gattatttct
     4741 cttaataaga atcgaagagg cgtttggcaa tatgttgttg atttaggtga tggcgttaca
     4801 gttgagtgta aagatagtca actgatcaga aataatggca gtcattccat tgaaaaagca
     4861 attcttgtat acccaaaagt atcaagtaag atcatgaatg actggttgct tggaaaattt
     4921 ggtgagtaca ataaaaaaga aatcgcagaa gtgatcggca aatcttatcc aacagtatta
     4981 gggtatctaa aaaagataac gagtaaagaa gcaaaaattg aggatgtttt aacagtaaga
     5041 gagcttcaat cattaatcga gtatcggaat aaacaaataa caaatcctga gtaccttaga
     5101 aaagaagatg gtctattgaa acaattggaa caagtaaata aaatccggaa gatgcaaaag
     5161 gtagctcctt taagccttga tacctttaaa aaaatctatt tgaaagagat caaaaaagac
     5221 taatgtggac tgtaacttta ccgttccaac ctgtatctgc accaagacca agtgcaagaa
     5281 ggaatgctgc tgatgaaact aatgacgtag cagagaaaag gatttcaacc tactatgata
     5341 aaaagtatct tgattatttg aaaaaaataa agagctttat cgaagatcaa gatttattag
     5401 atgatgaatt ttactcgatt gtaaatgatc ctatgggcgc aattatggaa gtggactttt
     5461 actaccagat accaaaaagc tataaaaaag tctataacat tatgaagaca acagcgccag
     5521 atttagataa tttagtaaaa agtgctatgg atggtatttt taacatctca gctatacgag
     5581 atagccatat cacaggctta attgctttta aatataatgt ggcgaacgaa ggaaaaacag
     5641 ttgtgaggat caaacgatat tcagaatttg agtgggatac ttctgaagga ttgaacggag
     5701 atatatggga agctacattt gattttaaac cagtagcaac gccaagacca aagtctaaat
     5761 ttagaggaaa tgggattatc acttactatc caaaagagta caacgactat ttggaaaata
     5821 tcaagaatac gcttaaagag aaagatttgg taaatgatca actgaaaaag gtaatgaatg
     5881 ctcctatggg ggttatagct cagatagatt atttttacca agtgagaaaa gataagaaaa
     5941 agcttgattc actgatgcgt acttcacagc cggatattga taatttagtc aaaggaacat
     6001 tggacggaat ttttaacaag gaaataggca tcaaagatag cagggttgta gggttaattg
     6061 cttttaaata caatactttt gaaaagtcga aaacaaacgt gagactacag gagatgggct
     6121 gatgaataat aatgaaaaaa ttcttcatgt acttgatagc tttgaaataa ttcaagaaga
     6181 actaaagaaa tatcgggatg ttttggaaca aagatatgat tttgtcaatc agcaaaagag
     6241 taatcacatg gatttcattc tcaatatgaa tgacttgaag aagaaattag ttgaacgaaa
     6301 agaacaagaa aagttgatca aagcttactt tgaactcggt gaaaaagaag ttaaaaatgc
     6361 aatggaactt aatgaagagc gcagggtttt agatcagctg ttagaacaat tattggtgat
     6421 gtttcaaaaa ggacgaatag atgaggattt aattgaagaa ggattgagaa aatatccttc
     6481 aaactcaggt atcggaatag tgctgaaagc gattgatgaa gaagaaattg aagctttcat
     6541 accgccagag gattttgaat cggcaatgga atatataaaa tattatagcc aagggatcac
     6601 tgcttttaga gagtttgatc ctgaagatgt tattcatgac ttgaacaacc taaaagaatg
     6661 gtgtgaaagt tatggagtcg atgattccgg attggactac ttgatcagta ttatggaaat
     6721 cgaagaagag atgccggata agcctgatcc aacagatatt ttagaattga tccatgaagc
     6781 tagaaatcca attgcctata tttctagagg atatactgta ttggaatact ataagcctta
     6841 tatatcagcg atgaaccatt taagacgtgt attaagggaa aaacgtgaat accgctcagt
     6901 gttgaatgca acgaatagat tagaaaaagc tgtttcagag ctagatgctt attatcgtga
     6961 acattacctg caagcaggtg gtatgccaag aaatacgaaa gcaaatatca gtaggtatat
     7021 aaaaaaagct gagtaggcgg agttagccca taaaatggca aaaggtaacc tgaaaggggc
     7081 attttgaatg aaaagggatg tttttgaatt gtacgtatta gaacgtctag aagacggcat
     7141 gatggcacaa gagataattg atgaaattga tgatatgatc agcagatttg gtcaattgga
     7201 gttgaatgat ggaaacaaaa attcggtagt gctaaaaaaa ttttggttga acaatttttc
     7261 gtataaggat tttatcaaag aaaaggcaaa tacattgtac tgactaaatt cacgaggagc
     7321 agtgatgaga gcttattgct aagagcagtt aggctctttt ttgtttgtgt aaaattattt
     7381 gttttataat acacatttat acacaaaaaa atattgacgg atagtacaca aatgtggtag
     7441 gatatatttg taagcaagtt agattaacaa gttaaaggaa ggaagagcag ttatgaaaaa
     7501 agaagaacta atagatatat ttcaaattgt tgaacgagca aataatatgg gaattatgtt
     7561 ttttgatcgt atttctttaa agatggattt aagcgttgcc caccaagaat ttaatctgcg
     7621 attaaaagcc ttgttgaatt ctgatgatgt taatttcgct catgatgttg taggtataca
     7681 gaaccatata gatagagaaa acaaacgtat gggtgatgaa ttcttaccaa gatattcaag
     7741 tttatgattt ggcgaaagat tcaagagtat gtgtcattat tgataggtac tcttgaattg
     7801 gaaaggagga atgaatttaa gacaacaacg attatgatct taaaacaatc gtttatgtaa
     7861 ttggcaatct tacgaataat tttcatgcaa taatgacaat aaaaaatgaa aaggggtaaa
     7921 atcaaatgaa aaaattttta gtaggaattc tattcacaat ggggctagta ttattatcac
     7981 cttcagtttc agcagaaagc tctatttcca tgtatcgtat gtataatccc aatagcggag
     8041 agcatctata cactagaagc gttggagaaa gagacaatct gaaacgttat ggatggcatt
     8101 acgaaggtgt cgcatggcaa gctccaacaa gtggtactcc tgtttatcgt ttgtataatc
     8161 ggtataacgg tgaacatttt tacacagttg atgccggaga aagagataag atttcacaca
     8221 caggatggac gtacgaaggt gttgcgttct attcctacaa cggagcaaat agagttcctg
     8281 taacacgttt atataacaaa tacgtgaatt ggcatcacta cacgttagat caaaatgaaa
     8341 agaacgtaat tagtaaacaa ggttggaaga ttgaaggtac aggctggtat gcaatgccaa
     8401 acagtgtacc aaatcctaca cctgctccaa gcactgaaaa agtgttgaac gttcctgttg
     8461 tatggcaagg aaatacaatg ttatgcgaag gagcttcttt attagaaggt ttacactaca
     8521 aaggtgtagc cacaaatcaa agcttaacca gttttgtaaa ctatatgcca aaagctaatg
     8581 acaataatcc ttatcatggt tactcaggag agtggcgtca taacgttaat ggaacgtatc
     8641 aaggcatgat ggctgaccca gtagttaaat gggcaaaaaa cgttggcggt aatgccgtca
     8701 atatcactgg ttcaggaacg aatggaatca agaatgaaat tcgcaatggt aatccggttg
     8761 taacttggat tacttataac tatgctacgc cagagttcaa acaaatgcct tggggtcgtg
     8821 cagtatggaa tggacatgta gttctcgtgg atggcttcag aaatggtgca taccatgttg
     8881 ttgaccctgt atttggcgca aaatggatca atgcaagcac gtatgagaga gcatttaatg
     8941 tgactggaat ggcggttgct gtacgttaat tgatagaatt atcaactaaa tgaaataaga
     9001 gcttaattac tgaaatggta aataggctct tattttttta gaggaaattt ttgtaatacg
     9061 aatctataac aaaaaatatt gaatgtagta cacaaaagtg gtatattaaa atcgtagaga
     9121 ttatgcgaag aggtgatttt gatgaaccaa agaagaagaa aaaggttaga agaaataagc
     9181 gagcgacttt ccgagctaaa ggatgagtta gaagtaattc aagaagaaga gtacaaagaa
     9241 aatataccta aaaatctaca gggatcagaa agatatgaaa aagcagaaga atccgttgat
     9301 caacttgaag aagctgttga tggtttatcc gacatattag aattgattga agatatagct
     9361 agctagagat gattaatgaa gatttcagga gtgagaataa aatgattgat atttcactga
     9421 tagagaagtt acttttctca aataagtaca ccgcaaaaga gctggctaca agatggcaaa
     9481 tatccgaatc agctattaaa gcctatcgtc aaaataaggg aagcgtagga tggcgtgatt
     9541 ggaaagggat cagcattgga aaagcagaag acataatgaa acgtgaagga cttcgatcct
     9601 ataccacttc tgaaaaagac aaataactta atgtttgaaa agataaaata tctgtcttaa
     9661 agctatgtca aattcaaatg gtgaatgttt gctaaatata atgcaaaaaa aggaagggaa
     9721 atattaaatg ataagcgaaa atttgtctta tgagaataat aaacatctag ttaaagaaaa
     9781 taagaaagta gaagattata agaacatgta tatagaaatg aaaaaatcac cactgtgtat
     9841 tcaatataaa aatgataatt ggaagcaagt agtcgaattt gtaaaacctc atagtatacg
     9901 aagagtacga agggggaaga aaaaaagtga acttgtaaat agcggaagag cggtaattag
     9961 agttataacc aaagattatc cagttgaata tggagattat attttaaggt taagttatca
    10021 tgcttttgtt gtgttgaaag aagagcattt caacgagttt ttcaatttaa aaagtaagta
    10081 atcatatttt tttgtagacg tgtataaagt taactaaatt gcgaggtgat gacatggtag
    10141 caaaaaagaa tactctaaaa gaagcttctg aaaaatatca agatatgaaa agacgaggat
    10201 taataccgaa agacaaaaaa ggtatatgga gagaatttgt taaggaagtc agtagtgatc
    10261 gaagtggcgg tgactttgtt ggacgatata cagcaaaagg ctatagcaaa cgattaagga
    10321 tcactgttga atatggaacg aatgaatcag ctacagagct gaagcaaagg tacttagata
    10381 cacgaaggga agcttcaaaa aaatataatg atttcaagag cagaggtctg attcctaaaa
    10441 agaaaaaggg aatttggcgt gagtttttca aggaagaaga tagcaatcgg aataagggag
    10501 acttcattgg acgatacaca gcaaaaggct acactaagaa tttagggatc aaggttgaat
    10561 acggaactgg tgaaagctca tgggaattga gaaatcgcta tttaaataca ttgttagaga
    10621 aatataaagc agaattattg gagttgttcg atgttaatga ttttatcgag atggctgaaa
    10681 agtatggtca tgaagaaggt tttatgatca atgacattat ggttattgat aattttacgg
    10741 atttattagc acttgcgaaa aaagcaaggg atttaagaga aacgcagatg tatctattca
    10801 gaaataaaaa cggaagaaac cggagaactg aatcgagtga gattgaagat tactacgatg
    10861 cctttacaca cgatctggga gcctttgtag acgagataag tggtgtaatg aacaaatata
    10921 gggtctagaa taaaaaagcc ggagatgagc agataaatgg gaaacaatag catatacaca
    10981 cctgaaatgc agaataaaat taaaagactt gtcggattag ggtggatgac aagaggaaaa
    11041 ttagattctt ttagtacgat caaatatctt ttgatggaca tttaccgtca gaacaagcaa
    11101 acaggaatac attttttggg ttatgataag ccggaagatg cacctttctt tgttccaagt
    11161 tcagcctata gagccagtac ctccggtagc atgtataact tggatttgtt tgaggaagga
    11221 gcaaagatga tcgaatatga tttcaacgaa gcctattcga ggatcatgag aaattatcct
    11281 ttgccgtcaa acacttattt gaaagcaaaa tataatacag atcaagctat tttgagagcg
    11341 ataaatagac caaaagtaag accgtatgaa aatttaaaaa cgtatatgtt ctttttggtt
    11401 aattttgaag gaacagccaa agaaggaaca tatgctaatc caaaatcaca gtcgatgttt
    11461 gcggattatg gcgaaaatcc caaaacagct aatgagccta tttggttatc ggagattgag
    11521 ctaacactta tccttgatta ttatgatatt aaagagatgg aagtcctaga tagccacaca
    11581 tttttgacga aaaaaggatt actcaaagaa tattttgaaa gaatcgagcc attaaaaact
    11641 gatcctgata cgttgaagct ttataaaaat cttagaacga aagtatttgg tgctatcgga
    11701 caaaaatcat taagagaagg agaaacaagc accttcgata aatacagtat gtataatcga
    11761 gcgtattcct ccatggtagc aggcgtattt agagatcgta tggttagata tgaacagaaa
    11821 tacgttcata gcgcatatgg gctattaaaa attcggactg atggattgta tttcagatcc
    11881 gaagtaccag agtttgaaga gctgtataaa cttggagtag ttaagaaaaa agttcataca
    11941 atatccaaag aagaagtcga aaaagcttta gaatttaaaa acaaaggatt tgaaaataag
    12001 ggctcctttg ggaactctaa agatgaagtt aagggtgttg tgatagacga tgatttcgag
    12061 tttgattttg actaaatact ctttggaaaa gatggtagga ggtaaatatg aagaagaaaa
    12121 ttgtggaagg aaatgtaggt gttattattc taaaagattc aactgactat gctaaatcaa
    12181 tggcactaaa agacgaaaaa gttgcacgca tggataaaga tttagttgaa tttctgatgg
    12241 atgatacaga aagattaaaa ggggatcagt ttttgagttt aatgaaagac aaaggcttaa
    12301 acacatatgg tttatttgat tataagaaat tagctataga gtgggtaaaa gaaggagaaa
    12361 tattttttat tgaagaatgg gaaggtactg agaacttagt tcttcatcag tatcttttga
    12421 aagcttaatc taagtaaaac agttctgact agttatgctt gtcagaattt caaaggatgt
    12481 tttccataac catcatcatt caaaaataaa ggatttgaaa ataagggttc ctttgggaac
    12541 tctaaagatg aagttaaggg tgttgtgata gacgatgatt tcaagtttga ttttgactag
    12601 atttttttga aaggtttaag agtaaacaag gtaagaacta agatcatgaa gattgagagt
    12661 aaagaagata gaggacgaat gtatgagaac acactatatt tatttagaag ataaaattgt
    12721 agatgaaatt ccggcaaaaa gtcgagaaca agctagaaaa ttatttattg aaaaatacaa
    12781 agagatcaat ggagatagtg aagttccaaa agtggtcgta acagttagaa aaaggcaaac
    12841 aaaaaagaga agtacgtttc atgtcagccc tgatatatat aatcttatag agcgacatag
    12901 aagtgaagat aatatttcta tctctcaata tattgctaca gttattgatg aattgatttc
    12961 aggaaagtat gactatgctt ttgattttga ggatgaaaag gattacaaaa gtgttgctgt
    13021 ctttcaatct gattttgaga agctaaaaaa ctatgctggc aggagaaatg ttaattttgc
    13081 aagactacta gaagagttct actccgaaga gttgggagta ggaaatgaat acaagagaga
    13141 aacttatgcg aaattgtctc ataagttatc ccaagcgctt aaagaagtga cagaagttaa
    13201 agcggaacta gatgctaaac ttgaagaagt gaaaaaatat caaacagaga ggaagaacaa
    13261 catgaagaat caagtggaat ttgacgaaca gaaagaattt gaaaaagcaa gaaaacgcta
    13321 taacaaaatt atgaatgaac ttagctatga acacttaaca gttcatgaag aattgagtga
    13381 gggtacagaa aaatatacga tcgcagatat ggttaatgaa gtggaaactc ttttagcttc
    13441 tttctttgaa aatggtcacg taaacgatga atggcgaaaa gaagaaccta aaagggttaa
    13501 cgcttatatt gctcgattaa aaaggtttat tgataaatat aaggagaata gtgacaaatt
    13561 ttctaaagat aaataaggag agagtgtgat tacatttgcc agaaatcgaa ggaatatttt
    13621 cgccaaaaat aggtaaaaaa cagttagatt tcagcagttt tccgagcgta aatagagatt
    13681 ataatctagg tgatgaggta cagatactaa atattgattt caaagaacta gacttcgctg
    13741 ttgctgtttc caacaaaccc aaggcattgg aacgagtgaa aaaaatatac caagagaatg
    13801 atcaaaatat caaaaatatg atcaaaaagt atagacgaga taattcaaaa gttgaagacg
    13861 ttttattcaa cggtacggca aaacatgatt tgaatgttta tatggcatta ggagtggctt
    13921 tgtactgtaa ttcaattaat cagacagata ttttggttaa tatcgtgagg aatagctatc
    13981 aaaaaatatt aaaaataagt agcgaaacaa acccttatta tgcaatgaaa tatcttgggg
    14041 ataatttatt aaatgataaa aaattgatgc acaacactga agagctgtta tctaatttgg
    14101 ctgcgattaa ctttctattc aaaacaggtt ctgaagatta ttttatagat aacgtactaa
    14161 aaggtgctta cggaacagca ttttatccgc tttacaatat gaaacagcat aaagccgaac
    14221 aaaaggataa caaacttgaa attaaaaaga tgaaagaact attaggtata ggatttgtaa
    14281 agcccaattc aaaagtcact attgcttcgt tagcaactac taacaatgat gggaaaatag
    14341 taaatgggtt tgacgattca gatatagata tattgtcaaa atcatatttt tctttgtatg
    14401 gagattttgc agacttgatg ggtattgata gttacttagt caataatttt gagatcaagg
    14461 gtagcgagct gaatgatatt gtgtatttcg ttttaatctc tctatatgca attgatcaga
    14521 ataccgagta ccgacaaatt gactatttgc tagtttttta tagtttgtta tatcagttcg
    14581 ttttaactcg gttgtttaaa aatgctaagg aagaactgat cctgaattct gattatgaaa
    14641 tagcaaaaca aattgcaagt attagaaaag aaaacaaatc tgaagtaatc aaattaagaa
    14701 aagaattaag tattgcgagt aaagaaaacg gtcggttaga atctttagtc aaatcggcaa
    14761 aaaaagaaga ggaacttttg aagcaagaga ttaaagaatt aaaaggaatc agaaaagaat
    14821 ttgaacagct gaggtttgaa aataaagtat taaagcaatt gcaggaacaa gaacatagac
    14881 aatcattaaa tggtaaaact gaagagcctt cactgtctga aatgatagct tatctaaaac
    14941 ataaaaacat tcttttagtg ggaggacatg taaattttca aaataaatta actgaactat
    15001 taccaaatat taaaatgttt gatgtaagcg aaaataatgg aaagatactt aaaaatttga
    15061 atacgtgtga tgcgattttt ctatatacag attacttgaa tcatggtcta tattatcgtg
    15121 ttatgagtga aatttctaat aaagataaga tattttattt ggataatcat gtgaatatga
    15181 acttaacaat taaacgtatt tacgatcttt tatcacttat ataaagagag aagtgataaa
    15241 taggacacaa aatttggaaa gtaggtaagt cactatgaga gaactaactt tatcttctga
    15301 ggaattagaa aagaaaactg ctttattaaa cgaaatattt agtgctgatt gtataatcca
    15361 aagtttaaaa gaaagttcat tttcaaaact aacagatcag aaaaaaattt cttatatgct
    15421 aagtttagat aatctaggaa ctaaatatga tgaatcggca agtagcgata agcttttgat
    15481 tgaattagta tcaagtctta gtagttttgg aaaaatgaaa agatttaatg tgatattaga
    15541 aatttttgct tggataatcc cttcatttat ttggtttttt tatattttca tagttgaatc
    15601 tgagttaata gccaaggtct ctcttgaact gttaattccg atatatgcat tttatttaat
    15661 aggttcagca atttacttta ataacttgta tacacgtatt caaataaaaa agca
//
LOCUS       DAFGZE010000065        15286 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.067, whole genome shotgun sequence.
ACCESSION   DAFGZE010000065 DAFGZE010000000
VERSION     DAFGZE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.067"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LTU54_002204"
     tmRNA           complement(43..409)
                     /gene="ssrA"
                     /locus_tag="LTU54_002204"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(580..1353)
                     /locus_tag="LTU54_002205"
     CDS             complement(580..1353)
                     /locus_tag="LTU54_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8296902.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            1497..1847
                     /locus_tag="LTU54_002206"
     CDS             1497..1847
                     /locus_tag="LTU54_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBL8296903.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            1828..2544
                     /locus_tag="LTU54_002207"
     CDS             1828..2544
                     /locus_tag="LTU54_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8296904.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            2544..3992
                     /locus_tag="LTU54_002208"
     CDS             2544..3992
                     /locus_tag="LTU54_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8296905.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4062..4571)
                     /locus_tag="LTU54_002209"
     CDS             complement(4062..4571)
                     /locus_tag="LTU54_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296906.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(4561..5286)
                     /locus_tag="LTU54_002210"
     CDS             complement(4561..5286)
                     /locus_tag="LTU54_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296907.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(5509..7629)
                     /locus_tag="LTU54_002211"
     CDS             complement(5509..7629)
                     /locus_tag="LTU54_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HBL8296908.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            7859..9037
                     /locus_tag="LTU54_002212"
     CDS             7859..9037
                     /locus_tag="LTU54_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HBL8296909.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9053..9811
                     /gene="aroD"
                     /locus_tag="LTU54_002213"
     CDS             9053..9811
                     /gene="aroD"
                     /locus_tag="LTU54_002213"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBL8296910.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFFSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            9834..10319
                     /gene="ybaK"
                     /locus_tag="LTU54_002214"
     CDS             9834..10319
                     /gene="ybaK"
                     /locus_tag="LTU54_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBL8296911.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10390..11643)
                     /locus_tag="LTU54_002215"
     CDS             complement(10390..11643)
                     /locus_tag="LTU54_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL8296912.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            11760..13109
                     /gene="gdhA"
                     /locus_tag="LTU54_002216"
     CDS             11760..13109
                     /gene="gdhA"
                     /locus_tag="LTU54_002216"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBL8296913.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13221..14567
                     /locus_tag="LTU54_002217"
     CDS             13221..14567
                     /locus_tag="LTU54_002217"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBL8296914.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(14875..15261)
                     /locus_tag="LTU54_002218"
     CDS             complement(14875..15261)
                     /locus_tag="LTU54_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HBL8296915.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         4990 a         2737 c         3104 g         4455 t
ORIGIN      
        1 aaataaggat ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag
       61 tcgaacccgc gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta
      121 aggattcact ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc
      181 tttttcaact tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg
      241 agcacatggg cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag
      301 agttgttttc gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta
      361 cgaaacgcaa ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca
      421 agggttacgt gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa
      481 aaaagcgaat catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag
      541 cgattttctt aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt
      601 caatgcgtgg acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt
      661 taatccgtca taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa
      721 atcattcgga ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg
      781 cagacgtttg acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg
      841 accattccga taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg
      901 agataagatc agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg
      961 aacatctgaa tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca
     1021 gattttctga tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat
     1081 tttttgacat tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat
     1141 gaaagaacca ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat
     1201 ttctgcttct gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt
     1261 catagctttt gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag
     1321 atcaaagaaa gtgattgcac gataatttga cacaaataaa actccttttt tattggaata
     1381 agtttatctt aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata
     1441 ttggttcagt caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga
     1501 tttttttcag tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct
     1561 caaaaggaaa tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc
     1621 attggcggct ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg
     1681 cattgatcgg ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc
     1741 ttctgctagt atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat
     1801 atgacctgga aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct
     1861 gtttctgaaa tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat
     1921 gatggaaaag aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca
     1981 ctagacctga atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt
     2041 tcaagtactg tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc
     2101 ggcttagaga tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt
     2161 gctcgaatga aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta
     2221 gtttcagatg atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga
     2281 gaagcgtatc tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg
     2341 tttactttag caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc
     2401 tgggattatc agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga
     2461 caaaaaatcg aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa
     2521 ttcgatgatt ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg
     2581 gaatggtcgc gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa
     2641 cgattgaaga aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa
     2701 cgcaaaccta tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat
     2761 tgctgctgac agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa
     2821 acgaacgtgt catttatccg actaatacag ctttactgaa ttttacctta acagctgatc
     2881 aatggaaaaa tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag
     2941 gacaagcaca ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg
     3001 gagcacttgt agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac
     3061 tgaacctgtt caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt
     3121 ttgcagcatt ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga
     3181 atggtaattt tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag
     3241 atgatttcaa taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag
     3301 aaaatcgtcg aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga
     3361 caatcaacgg acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa
     3421 acgcgattaa attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact
     3481 tggattatga aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa
     3541 cagaaacatt gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata
     3601 cattgacatt gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc
     3661 aaatcatggt caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga
     3721 ttactttaga aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa
     3781 tgtctgaaca acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa
     3841 aaaatacaaa atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct
     3901 tgcataaagg aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc
     3961 gtttgcctga cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat
     4021 cgatcttcta taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa
     4081 cgatcaatat aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga
     4141 ttttcttcat gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta
     4201 agttcatcaa atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt
     4261 aattgcagct ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa
     4321 ggagagatat caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt
     4381 acatccaaca ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg
     4441 atgatcgcaa aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt
     4501 ttatgctgtt tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat
     4561 tcaggtttca ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg
     4621 gaatagacta agaatgagac agcatccact gagcccaata agagtttggg caaatccctt
     4681 aagtatatct gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc
     4741 catcggaatt gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt
     4801 agtctttaag acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct
     4861 tacgatccac aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa
     4921 aataaaaagg aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg
     4981 cttgttttgc ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa
     5041 taaactgaat atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc
     5101 tgtagatgca gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga
     5161 ttgcaaatgg taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa
     5221 acaatagagt attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat
     5281 tttcatactt ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt
     5341 cctttagtga aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga
     5401 agaagctgcc ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag
     5461 aggagcagcg acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
     5521 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
     5581 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
     5641 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
     5701 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
     5761 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
     5821 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
     5881 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
     5941 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
     6001 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
     6061 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
     6121 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
     6181 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
     6241 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
     6301 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
     6361 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     6421 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     6481 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     6541 gaatgtacca gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat
     6601 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     6661 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     6721 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     6781 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     6841 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     6901 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     6961 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     7021 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     7081 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     7141 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     7201 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     7261 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     7321 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     7381 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     7441 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     7501 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     7561 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     7621 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     7681 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     7741 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     7801 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     7861 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     7921 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     7981 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     8041 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     8101 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     8161 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     8221 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     8281 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     8341 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     8401 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     8461 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     8521 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     8581 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     8641 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     8701 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     8761 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     8821 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     8881 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     8941 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     9001 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     9061 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     9121 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     9181 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     9241 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     9301 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     9361 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     9421 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     9481 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     9541 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     9601 ggagttgact agacgggcaa atgctttttt ctccatgcct attattacga tgtccatggg
     9661 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     9721 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     9781 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     9841 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     9901 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     9961 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
    10021 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
    10081 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
    10141 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
    10201 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
    10261 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
    10321 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
    10381 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
    10441 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
    10501 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
    10561 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
    10621 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
    10681 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
    10741 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
    10801 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
    10861 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
    10921 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
    10981 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
    11041 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
    11101 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
    11161 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
    11221 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
    11281 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
    11341 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
    11401 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
    11461 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
    11521 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
    11581 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
    11641 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
    11701 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
    11761 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
    11821 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
    11881 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
    11941 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
    12001 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
    12061 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
    12121 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
    12181 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
    12241 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
    12301 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
    12361 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
    12421 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
    12481 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
    12541 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
    12601 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
    12661 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
    12721 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
    12781 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
    12841 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
    12901 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
    12961 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
    13021 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
    13081 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
    13141 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
    13201 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
    13261 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
    13321 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
    13381 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
    13441 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
    13501 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
    13561 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
    13621 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
    13681 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
    13741 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
    13801 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
    13861 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
    13921 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
    13981 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
    14041 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
    14101 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
    14161 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
    14221 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
    14281 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
    14341 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
    14401 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
    14461 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
    14521 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
    14581 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
    14641 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
    14701 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
    14761 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
    14821 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
    14881 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
    14941 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
    15001 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
    15061 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
    15121 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
    15181 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
    15241 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DAFGZE010000066        15140 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.068, whole genome shotgun sequence.
ACCESSION   DAFGZE010000066 DAFGZE010000000
VERSION     DAFGZE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.068"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(9..1644)
                     /locus_tag="LTU54_002219"
                     /pseudo
     CDS             complement(9..1644)
                     /locus_tag="LTU54_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2199..2762)
                     /locus_tag="LTU54_002220"
     CDS             complement(2199..2762)
                     /locus_tag="LTU54_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL8296916.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2777..3820)
                     /locus_tag="LTU54_002221"
     CDS             complement(2777..3820)
                     /locus_tag="LTU54_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296917.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3953..4357)
                     /locus_tag="LTU54_002222"
     CDS             complement(3953..4357)
                     /locus_tag="LTU54_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HBL8296918.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4590..5225)
                     /locus_tag="LTU54_002223"
     CDS             complement(4590..5225)
                     /locus_tag="LTU54_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL8296919.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5491..6543)
                     /locus_tag="LTU54_002224"
     CDS             complement(5491..6543)
                     /locus_tag="LTU54_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296920.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6556..7494)
                     /locus_tag="LTU54_002225"
     CDS             complement(6556..7494)
                     /locus_tag="LTU54_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL8296921.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7945..8595)
                     /locus_tag="LTU54_002226"
     CDS             complement(7945..8595)
                     /locus_tag="LTU54_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296922.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8695..9204)
                     /locus_tag="LTU54_002227"
     CDS             complement(8695..9204)
                     /locus_tag="LTU54_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBL8296923.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9405..10274)
                     /locus_tag="LTU54_002228"
     CDS             complement(9405..10274)
                     /locus_tag="LTU54_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8296924.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(10294..11415)
                     /locus_tag="LTU54_002229"
     CDS             complement(10294..11415)
                     /locus_tag="LTU54_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HBL8296925.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(11417..11812)
                     /locus_tag="LTU54_002230"
     CDS             complement(11417..11812)
                     /locus_tag="LTU54_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL8296926.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(11831..12637)
                     /locus_tag="LTU54_002231"
     CDS             complement(11831..12637)
                     /locus_tag="LTU54_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8296927.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(12630..13418)
                     /locus_tag="LTU54_002232"
     CDS             complement(12630..13418)
                     /locus_tag="LTU54_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296928.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(13432..13908)
                     /locus_tag="LTU54_002233"
     CDS             complement(13432..13908)
                     /locus_tag="LTU54_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296929.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(13980..>15140)
                     /locus_tag="LTU54_002234"
     CDS             complement(13980..>15140)
                     /locus_tag="LTU54_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8296930.1"
                     /translation="FIQIGDIYLTKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLT
                     ATCIASTANRIVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLE
                     KIYLNLELDAQKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESS
                     NIKKDMIVCSCASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYLSELRKEN
                     NILFIVGTLNPHLEDVEFFSIEDMILGMNISKFSNQLNRHFNDNELKVLNNNILKNFS
                     LTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFGLYVHISCLIERLVKQN
                     TLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
BASE COUNT         4810 a         3118 c         2268 g         4944 t
ORIGIN      
        1 aaatgtaatc agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa
       61 agaaagaggt aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg
      121 gaagaaactt caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc
      181 aatctctctt ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag
      241 cgttattttt tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg
      301 ataaggcata gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt
      361 gtattgtaat gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta
      421 acgaataaga ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc
      481 atagtttgct tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt
      541 aactactttt ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga
      601 gccaaacagt acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt
      661 attttgtaat aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact
      721 aaggtaaatc actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa
      781 agaaataata ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat
      841 caaactatcc gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat
      901 cgattgaacc atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt
      961 cattcctact gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc
     1021 acttactaca aagccgccta agactagtaa gccaacggct attacactca taaaagtgga
     1081 aagaacatgt gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt
     1141 tttctcataa atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat
     1201 cgcttcagca atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata
     1261 taaacagcta aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa
     1321 catcagcatg atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa
     1381 attattactt atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata
     1441 attcactccg ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga
     1501 agataaccct ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg
     1561 aacatttcta aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa
     1621 tattattttt ttctttactt gcattactta attatatact cctttcatgg attgctttat
     1681 ctagtataac ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca
     1741 ctaaaattat aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat
     1801 tgcttaacta atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt
     1861 gcgatctctt tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg
     1921 gatatgacta tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag
     1981 gcgctgcgta tcatgagtga atgatattag gaaaacttca taaaataaat cttttataca
     2041 tttccacttc tagatggtca ttcatgttag aagaatcaaa ctattttttt actgtcgtaa
     2101 taatattaaa acactagaac aaatcaatta aattccataa aatataaata atagacctag
     2161 catcatctgg tataaaatgg atgctaggtc cgttatattt actcaaaatt gattctacga
     2221 atagatcttc ttatcatttc tattttagta gtcatgcgtt gttgttcttc taaaggtggt
     2281 aatggtataa gtagtttaga taaatttgta ccatttacat ttggttgccc agttccgtaa
     2341 gaaatttttt ctaataactt ccaatataat ggcgagtcta agaagcagtc tacgtattct
     2401 gataataatt tctcaactaa cctaattcga atcaagtaag aagcaaaaac agattcctca
     2461 cttatttcct taaccaagaa actttttccc atagttcctc ctgtccttgc tatcaaaatg
     2521 tcattttctt ccaatcttaa atcaactagt ttagaattac taatatcaca gtaaggcaca
     2581 ctggaccaat ttacacgtcc ctcttgtata tctgtgattc tcaaaaactt aacattgcct
     2641 tgttttttcg ccgaatctgt atagccatat tgaatcttag tagaaatact tcctaaataa
     2701 acccatagtc gaacatcgct agatgatttt tcttgtcgtt ctttatgaac aacagtgatc
     2761 atcttgagtt gtccgactat tttttagttg cagcaatctt tttgggacgc ttatttgcat
     2821 gaagcaacgt tgctaagtct agtaataaaa cattggctaa cacaggattg gctttctcgc
     2881 gatacgctgc ggcgccactt tcaaacaata ctgctagacg atcttctaca acaacgattt
     2941 gttccaaata cggtggaaat ttgattcgtt ttaggatccg gctaggagag tcccgctcgg
     3001 acactaagag ttccgaggta taaggaccat aagaacgact ggctaagaga taatggccat
     3061 aaaaagccac accttgaatc ttatcagatg tttttctgct cattgtcacg gttggttgga
     3121 ttttgtcttt gcggcgaatt gctgttttca tttcagctat cgtcggcaat agatagaaag
     3181 tcaactgtcc ttcacctttt agtgtgaagt tgccagatac cagttgatca ttgttttgag
     3241 tcaaatacga tgcttgtgga atctctgaga gatcagttgt cagccaataa gcgattggct
     3301 gtcccgtctc ttcagatgta tctgctaaga tatcgatcaa acgtaaagcg gctactcgtc
     3361 cagtagcaga tcctgtgatt gtcaaccata aaagttcccg ttttgtatca tatactaagc
     3421 cccctacatg tggccgttta ggtaaaatca gcagtttgat cggctgtcct gtttctctat
     3481 ccagtacatg aataatagaa tgatgctgat ggtcatagca gtaggcagaa ataaacacaa
     3541 aatcttgaga aaaagccaac ccttgcggcg tcatccattg acaaacctca ttttcgccgg
     3601 tttccggcac caccattctt gtttctgtta atccaggaat gttatattgt tttgcttcag
     3661 ccttttccca agctgcttga acaataggat accgttttaa ctttttttca atatcctgat
     3721 gtatccgttg ctgcttccaa tgcttaaact ttaggacata gtaaacaaga aggccaatga
     3781 ccagcaacac taaaatcatt attgctaccc gctgtgtcat ttttttgcct ccttgttcta
     3841 ttcgatcaat cgtccatata ccgctattaa ttaccgtaac tacatgtttc ttttactagc
     3901 gtttctttat aggatctttc ataatctcat ttatagtaac attaagaatc ccttattcta
     3961 ctatagtcac ttccacatca atattgcctc ttgttgcttt agagtatggg caaaatgcgt
     4021 gggcttcttc tgcgattttc tctgcttggt ccttctcaag tcctggcaaa gaaacttcta
     4081 aaacgacccc gatttggtag cccccatctt ctaattgaaa taaagatacc cgtgctttga
     4141 cttcagaatc agactcaaga ttattttctt ccagcatgtg ttgaaccgcg ccgttgaagc
     4201 aagatgcgta tccagccgca aaaagttgtt ccggatttgt cccttcacta tggattcctg
     4261 ggcggtcgat cttcatgtgc atacttccgt ttggtgcctc aacttcgcca tcacggcctc
     4321 cacgattgat cattgctgtt tcgtacattt ttttcattga gatcatcctt tctttcgtcg
     4381 ttctatatta agtgtaacga gtatttttta atctgacaaa ggtaatgctt gcattcggcg
     4441 acatcagtta gacaacatcg atgatcagtg atagaagcag tttatctata caaaaaaaga
     4501 agggttctat gatatttggt gtccttactc gccttgagta acaacaccaa atatcataga
     4561 accacttaat cagcttcaca ctgctttttc tactttcgta gttgaaaaaa acttaaacaa
     4621 atcatcggca aatagttaaa gaaaaaactt aatcgttgaa agaagcccaa ttgcttaaag
     4681 aaggcaaact gactaatcga cggaatccgt gctaatccgt aaagacctgc aattagtaaa
     4741 ctaataactg ttaaaacaag ataaaaatgg ctaagcgtac cttgaccact ttgacgataa
     4801 agcagaacaa gtaacaatgg gaaaagtaga aagcctgcat agcctagccc agaacctata
     4861 ttatggatcc aagtcgatag attacaagag ctttcattcg tattgatact gaataagcca
     4921 gtaaaaatac aatcacctat cccatataaa ccaactgttg caacaacaag tattgcaagt
     4981 gtttttgatg ttccaacaaa taggtgatac aacgcaggca acgaaagaac aaaaaaaagt
     5041 ccagatacaa ctgaccagac caagaaagca cgtctgactg gactatctac gtcaccaaac
     5101 acactaatta cggtagtcat ttgattcagt ttgggataaa aaaggccaag gatatacggt
     5161 gtcgataaat cactcatcac acctaataat aaaaaataaa aaccatattt ttgcaaaaaa
     5221 atcattttct tcccctactt tttagtgatt attcgataca tccaacaatc aaattttttc
     5281 tcattttaac acatattttt attctagttg tcttttgaac aatcaataac ataaaggtga
     5341 gtttcttcta cattttaaaa aatcgaatgg cactgaagag ttcagttgtc agcatcaatc
     5401 tcctactcaa aaaggcaaaa aaacgtaagt cagatgttgt tgacatctca cttacgcatc
     5461 cattgattga aaatttctta gtaaaatcta ctagcctaaa aatttgaaga cttcttctcg
     5521 atcatacagt ttaaatactt tctcgcataa aaattgagta attagtgccg caaaaacagg
     5581 gatgactaac caacaaagaa caacaagaaa gccatttagc cctgcatcta aagaagccaa
     5641 cggaccaaca agtccgacca cgccgaaacc agcagaagca ggcgtacctg aaatagaaaa
     5701 tagagcgaca ggaatagctg taatcactgc actaactaaa caaggaataa gaatcacagg
     5761 ttttctaaat agattaggca tcatcatctt catagcgcct aaagcaatcg caattgtcac
     5821 acctgactta ttaaccttcc aagagttcac tactaaaaca aaagtggttg cagctacgcc
     5881 catcgcagcc gcaccagcag aaacaccatc tagttggatt gccattccga ttgctactgt
     5941 tgaaatagga gagataatca aaaaggcaaa ggaacaacca atcaagatag acattaacag
     6001 cggttgtaag gtagtgaagt tattaatcaa tgatccaatt gcagaagtaa ttgctgttac
     6061 aaaagggaat aaataaaaac caagtaaacc tgcacctaca cctacaacaa tcggtgttaa
     6121 cacaatcgac actgacccaa aactatttcc aatcagtaaa atcagcccga cagcaatcga
     6181 tgctgtcagc atcgtattaa taatatcgcc tgtaccagca ccaacgtaaa cacccgtttc
     6241 acccaaatca ggtgtgaatt tgactacacc agaaccaaca aaagaagctc cggctacaac
     6301 catcattctc atcggatcaa atccaaattg taaggcaatc aaaccaccaa tgatcaaagg
     6361 tgttgctagt tgaaaaataa ctgctaactg tgcgatcgtt accgcaaagg gatacgcact
     6421 aaaatatttt aaaatggcac ctaatactgc attaggaatc aatccaataa tgattcctgt
     6481 tgctgtacca gcgaggactt tattcagaaa aattttcggt gatatttttt gtgaagtttc
     6541 cattcaatct ctcctttatt ttacgttcaa actatcttct tttgcatgga caagctcagt
     6601 caagaaatca caataaggcg tcgcaacacc atatttcttc ccttttctag aaattgcgcc
     6661 attgatataa tcgatttctg ttaatcgatg gttcttaatc aagtcttgat acattgaagg
     6721 ataatgtaac ccaattgttt ccgggtcaaa acaagattca caatgtgcaa tgacttctgg
     6781 gacatctagt tcaatcttct ctactgctgc tactttggca aactcattga caatcgtcgc
     6841 caccatttta tgagcagtcg atgtttttcc tagctcagcc atattaacgt ctaagattgt
     6901 gcatagtccg ttcattgttc cgttcacaca tgctttgcga taaatagaat agtgaatatt
     6961 gtcagaaaaa tgagcattta atcctgattc agacaatttg tctgctaatt tttttgcagc
     7021 tgcttctttt ccatcaccta aattttgtaa ttctactgaa ccacttccaa ataatttgac
     7081 ctgaccagga ccttcaagac ctgccgtcca catggtattt ccaatataga tattttccat
     7141 tggtacaaat ttctcaataa tatcttcatg cccgatacca tttaatagac ataagacctc
     7201 tgtatctttt ttgattaatg attggatatc ctgcagcatt ttttccagct gcatcgcttt
     7261 ggtaaataga ataatcagat caacttgatc ttctttttct acttcggatt gaagaacgat
     7321 tggtagtttt gcttctacct ctttaccatt aaaatttgct tgtaatccat gttctttgat
     7381 ttgttggaca tgttctgccc agccatcgat taataatact tcgttcccac tttggtgaag
     7441 catcaaccca aagcgacttc ccattgcgcc agcgcctgca attgctattt tcattgattc
     7501 ttcctccttt ttctttctat tatactcatt tgagaatctt ttcacaacct cgttttgtta
     7561 ttcttctctt atttatcatg tttatatcca ccattcaagt tattttatca gcaaatcaat
     7621 aaaaactgga atagaatccc aatttcatcg taagagaaac tagactagca ataagaaaat
     7681 agaaacattc atagaaaaaa tctcactcct ctgattataa aagagggggc attaacctcg
     7741 tgtatactaa aaccttccat tcattcgatg gatgattttc tttttgtgaa aaaatattca
     7801 ttgtagtacg atgttgcgca acacagacgg atggtattgc ttattattta ttaaaatcga
     7861 acaatacact tatttaaaaa cttctttccc agattttctt ccatttttac agttataact
     7921 agtattatca tgaaaaatag ccttttattg tctatgggaa taataattta aaattggtac
     7981 ttttttatta attagttcat cgcccaataa tctctccagt ttcttagcat ctcctgactc
     8041 aaccggaatt atccaagccc agtctaaata attttttaaa atctcatcat attttgcaaa
     8101 tatttcattg ccatattccg tccttaaaat tatattttca gtgccagaca agtgatttat
     8161 attgagttga taaaaaagat ctccatctaa aaaaatatca ttagaagaag atttccccac
     8221 agtaatgacc attccatcag ctctatgtgt tacatacaaa taattgtatg acctcatttc
     8281 acctaatgaa tcaattattt cttgcccttt agctctaata tattgatctc tattacctat
     8341 ctcactctta tttaacaaaa gttttttcat gtcaaattta atcggctgtt ttatttttgt
     8401 aaattcgctt ctactactaa tccaatccga gattaatttt tcaacatgtc ccagagtaaa
     8461 ctctgattta ttcgctaaat actttttttg ttgtattaaa gacatctttc cccgaagctc
     8521 aaaattgagc ctacatcgat tctctatctc ttcaaaaagc atcttatctt catcattctt
     8581 atattcatca ttcataaaac caatccagct cctcgattaa gataatattt taatctcata
     8641 caaaaactag ctctcatcaa aacaaccatt gttttgattt ctcatgaaat ttctttatac
     8701 ttttctccga ttataaattc ctaacccctt gagaaaatta cgcatatatt tatctccgca
     8761 aggcttaaaa tttcgttggc cttcttttct caaaaatgcc ccgatttcgc cttttgatgg
     8821 atagatttct gcctcagcaa aaacgtctaa aatatcatca gtggtcatag acaaggcgat
     8881 ttttaatttt tttagcaaca cattgttgat atatttgatt tccttgcttt tttgcggcgt
     8941 gtctactatc ggcttatctt tttgcggacc acgcgccaag agaatcaggc catttaggaa
     9001 tgcgtcaaaa acttggttgt tgatggtttt ttcatacaca ttattttctg cattatcttt
     9061 gttcttatcc tgtactttgg tcagtaccgc ctgagcttct tctttagtca gtggtacacc
     9121 gcctagctca aaagctttaa tcaaatccgt atctttcaaa tcaatcgcat aacgcaatcg
     9181 caataaccta tcattgttat tcatttgttc ctccgaagta tttagtcttt actttcttac
     9241 gtactttttg tcttgctaag atttttagga tcaatgataa aaaccgcatc acttatgata
     9301 aggcgctggg tatcatgagt gaatgatatt gctgaataca aatagtccga ccacattaag
     9361 ttgatcggac ttcataagca ttttattgac taatactttt ttgcttaaga attgattcta
     9421 acatcttttg ctaaagttaa gcatcctata gttcctgcat tatctgctaa cttaggtgtg
     9481 acaatgtatt cttcgatatt tggcgtatcg acatagccgt taattagttc taaaaaacgt
     9541 ctttgtactt tctttttcag atgttcttgt tgcatgacac caccaccaaa aattataata
     9601 tctggtgaaa gcatcagggt cgtattacag gcacattgtg caatataatc cgcctcgatt
     9661 tcccagaacg gatcttctat agaaagatct tgtcccttgg ttcccgtacg catctcgata
     9721 gcgggtccag atgccaaccc ttcgagacaa tcatgatgaa aaggacaagt accagagaaa
     9781 gtatctcctg aatgtctctt aactaacgta tgccccattt ctggatggct aaacccttca
     9841 acaaaaatcc catcttgtat agctcctcca ccaattccag ttccaacagt gtaataaaca
     9901 acactggata aaccttttcc atgacctgaa acatactccc cgtaacaagc agcattcaca
     9961 tcggttgtcc aggcaattgg aatttcgaaa tgcttattca acgttccaac aaaatcaaaa
    10021 tttttccagg cagtttttgg agtagaggtg atatgtccat aggtctttga ttcacgatgg
    10081 atatcaatcg gtccaaatga ccctactcca atgctttcta attgttcttt atagtgatta
    10141 aagaaatcaa ttaccaaact cattgtttca ttcggtgtag ttgtaggaaa actcactctt
    10201 tctaaaacct ttaaatcatc attgccaatg gcacaaacaa atttagtccc acctgcttct
    10261 attgagccat acatttctta ctccaccgtc cttttatcta ggaattcgtt cttccctgtc
    10321 taatagtttt tcgaaaaact catgtggttc cttttggtac caataggcaa cagaagaaat
    10381 atcgtcttga cgctcataaa gcttgatgtc atcatttcct agggtttgga aagtaacacg
    10441 taaattttct ttgaaactaa tggggtcggg taaatgccag cggtacaacc catgcatcgg
    10501 taaagcatca tcgccaaaac catgaactgg atttggatta ggttttccag cactaaaata
    10561 atcccgggtg tgatctcttt tagattgaaa aggatagccc ataaatagcg tattaaaatt
    10621 ttgagcatat gccctttctc caaagttctt tttatggaaa gcccaagcgc cgccaaaata
    10681 atcttctgct cctgtagatg ttattgtagg gaaatcttca tctccatcta tataaaactt
    10741 aaattctcct tccccccacc agtatctttc caatgcgcac aaagcaaagt aggttcctac
    10801 ataataccca gtgcctttga ttccatcaat aagggtgtaa tcttttcctt ttgtcgtgat
    10861 gggttcacgg ttccatttag catgaaaata catcgcatca tccggtactt cagtcaatgc
    10921 ataattaaat gtataaaaaa tgtgcttaat aaactcagga tgttcattcg ttaaagtaat
    10981 cttagctttt tttcgaaatg gcatctcaaa atagcaattc atccctgatg ttggattcac
    11041 tacgattggt aaggaattca cttcgcatct tgtcccaaac ccattacaaa aaaaatctcc
    11101 taatggactt tctacagaag gagtggattc atcatcccaa taaatacgca aaattaaatc
    11161 acgtaagacg aaactcccag cgattgtttt gtctgtcact gtcatccaaa aatgacgtaa
    11221 aattccaggg ccttccattt ctgctaaagt aattgtctct ccagcattta tcggaacaga
    11281 aggtgaccct tttctcccaa ttcctagatg actcgctgtc attgccgctt tccctttttc
    11341 accattttta ttttctggtg aaatagttct actttcctct cccttgtgga gccatatatt
    11401 atttaaacaa ttcattttat tcgtcctcta tcctttcttc acttttttga ttattcatat
    11461 agatgatttg ttgttgtgcc tccgcaataa ttgctccaat atctaaatta tcatttccta
    11521 actgcaaagc gattgacatt gccaagggaa gattcattcc tgcaattacg tgcactctgt
    11581 cagaaatata ggtaaaaaac ttttgattta cacttcctcc agccatgtca gttaaaacaa
    11641 ttatttcgtc tgtagtatcc actaactctt taatttgttc atctatatca tcaatttctt
    11701 cgacatatgc acataagcta ctaatttgat tatgcatacc tgttaaaaac tctagtgtct
    11761 ctttaaaacc ctcagctagt attgaatggc tagcaatgat caatcttctc ataattcaaa
    11821 tccttttcct ttacgctaat attccaaatg cagccataac catcgcaaaa acaataacga
    11881 gtagaatcaa aatggtcata ctgatttttt tcttactgag tagataatat gctgcagcag
    11941 taataattac tggaattaat gttggtaaaa ttttgtccaa cattggttgg attttcatac
    12001 taacttcacc aaaagtgaat ttaagtggcg ttgaaacact gataactgaa gcaattaatg
    12061 atccaactac tgttagcccc aatactgaag cagcttcagt taaataatta atcttttcac
    12121 cgtattcagt aacaatcttc attccctgct tataaccaaa ttcaaagcct ctcattcgga
    12181 aaaagaagat taccatgatt aaaatgaacc ataaaaagat tcctacagga ttattttcta
    12241 aagccatata agccgcaatt gatcccatga tggttgggat catcgtcatc aaaagagagt
    12301 ccccaatacc agcaattgga cccatcgtac caactttaaa gtcattaatc gcatcttttg
    12361 cttctaatcc ttgcttttcc tccattgcaa cacaagcacc taaaataatg gctgccagcc
    12421 aaggctgtgt attgtaatac tgaaagtgat tatttaaagc ttcgatgtat tcatcatcat
    12481 ctttgtataa ttttcttaaa ataggtgaca atgcgtaagt cacagatgca ccttgctgtg
    12541 tttgataatt aaaggtacaa actgacatca accaacgata gttcgctttc ctaagatcct
    12601 ttttcgttac ttgatatttt tcgctcattt tattcatcct ccccaataaa cccaacatca
    12661 gaagctcccg cagaagcagt gatttgtgca tctttttcag ctctatgata cacattaaat
    12721 gctaaagcga atcctatgat cgccgctcca ataataggta ccttcaaaaa agcggctaag
    12781 aaaaatccaa caagtaaata actaatgtat cgacctgtag gcataaagcg taacaacatc
    12841 gcaataccaa cagcaggcaa catttttccg gctaatgtta gcccatccat aaaccaatta
    12901 ggtaaaacat ccaaaacgta attaacagca gactgtccta aagtaacggc aataaatact
    12961 ggaattgctg cagataaacc aaagaaaatt ggccccaacc acaaaacact attcattttc
    13021 ttaaattctc gtttattcgc atattcctgt gatttgcgaa caacaaaacc atttaagatt
    13081 ttgacaatta catctagttg cacccctaac atacctacag gtagtcctat tgctaatccg
    13141 gcttctactc caagctggct accaaatgcc gttgcaataa ttgcgcacgt tccatagtcc
    13201 ggtacagatg agccaccaaa accggcaact cctaatgaca tcaattgaac tgtaccgcca
    13261 actatcaaac ctgtttttat atcacccatc aaaattcctg tgaataatcc ccaaaaaact
    13321 gcgtaattta caaagatcat cggaatccca taataatcaa catgtttaat aaatgctaat
    13381 atagtcagca taataatttg aaaaatagta aaatgcatta tccttactcc tttatcctaa
    13441 ttttgattga atttcctgtg ccttatccgc cggaacaaat tgtgtaacaa tttctacacc
    13501 agaatccaac aattttttat acgtgctgat attttcatct gatgcatagg ctctagaaga
    13561 tagctgtaat gcattatctt taacaacagt accgccaaca attagtcggt caattctaat
    13621 accgttatct actaaatgaa ggattgtttc cggctctttc acgatgacaa atactttttc
    13681 ctgatcgtat ttcccctttt taaaattcgc aatcgctgtt tcttcagtga taattgatac
    13741 agccattcca gcaggtctag ctaaacgcat acttgatttt tggatgtcgt tatttgcgac
    13801 agaatcatcg atcaccatca ctctgtttgc tttagacatt ggtgcccatt gcgtgacaat
    13861 aatcccatgc aataaacggt tatcaactct cgacataaca atactcataa atacttcctc
    13921 ctttatcttt cattttctta caagcaatta ccatgccaaa acaaaaagaa cttgataaat
    13981 caagctcttt ttacataatc ataaacgtat ttaacctcat gaatcgggat gtcgacacta
    14041 taataatgtt caacgacact gaaagattgc ttaaaatgtg tctgaaattt ttgaaattcc
    14101 tcactcgttt cattgaaata aatttcatct tccaaagtat tttgttttac taatcgttca
    14161 attaagcaac taatgtgaac atataatccg aaacacgtat tattatcaaa tgaaattcct
    14221 agatcatttt gtaagatgta tatagcatct gaaacccgtt ctaataattt agtcggattt
    14281 aatatggtca attgctccat taaattcgtt agactaaaat tcttcagaat attattgttc
    14341 aaaactttca gttcattatc attaaagtgt ctattcaatt gattggagaa tttacttata
    14401 ttcattccta aaatcatatc ttcaattgaa aagaattcta catcttctag atgtggattt
    14461 agtgttccaa caataaaaag aatattgttc tctttacgaa gctccgataa atagttttca
    14521 ttcactaaag agtaatagtc acacgtttct atgacaagat cagtatccct tggaaagcta
    14581 tcttcaagta tcctttttaa tttaaacgaa gttcctaatc cgcttgcaca agaacagaca
    14641 atcatatcct tcttaatatt tgatgattca taaatataat ccacattttg attttctttt
    14701 acttgttcaa gaatctgttt aagcggtaaa tctaataata tctgatttgc aacatcaata
    14761 gccattcttg ttgaaacatt gtttagtagt ccgaattttt tttgagcgtc tagttccaaa
    14821 tttagataaa ttttctccag tgaacccata tcaacaagta aaataatttc ttcatatccg
    14881 gtacactctt gtaagtatct attaattttg tctgcaatct cactacttga agtcattagt
    14941 ggcatatcaa tggcgtcgta tatatatctc tcaacgatcc tattagcagt acttgctata
    15001 caggttgccg ttagatctcc atgagccaaa atcagtgcta aacgtttttg ctgaaattct
    15061 ttactacaca ttaacttaaa taaaataacc aaaacatttt tttgaatttt tgttaaataa
    15121 atatccccta tttgaataaa
//
LOCUS       DAFGZE010000067        15057 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.069, whole genome shotgun sequence.
ACCESSION   DAFGZE010000067 DAFGZE010000000
VERSION     DAFGZE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.069"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            22..531
                     /locus_tag="LTU54_002235"
     CDS             22..531
                     /locus_tag="LTU54_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8296931.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            624..1786
                     /locus_tag="LTU54_002236"
     CDS             join(624..900,900..1786)
                     /locus_tag="LTU54_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8296932.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLTPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2066..3046
                     /locus_tag="LTU54_002237"
     CDS             2066..3046
                     /locus_tag="LTU54_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8296933.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3168..4586
                     /locus_tag="LTU54_002238"
     CDS             3168..4586
                     /locus_tag="LTU54_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HBL8296934.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4657..6126
                     /locus_tag="LTU54_002239"
     CDS             4657..6126
                     /locus_tag="LTU54_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8296935.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6136..6603
                     /locus_tag="LTU54_002240"
     CDS             6136..6603
                     /locus_tag="LTU54_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8296936.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6639..7511
                     /locus_tag="LTU54_002241"
     CDS             6639..7511
                     /locus_tag="LTU54_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8296937.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7593..10127
                     /locus_tag="LTU54_002242"
     CDS             7593..10127
                     /locus_tag="LTU54_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8296938.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10283..11056
                     /locus_tag="LTU54_002243"
     CDS             10283..11056
                     /locus_tag="LTU54_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8296939.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11049..11840
                     /locus_tag="LTU54_002244"
     CDS             11049..11840
                     /locus_tag="LTU54_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8296940.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11850..12341
                     /locus_tag="LTU54_002245"
     CDS             11850..12341
                     /locus_tag="LTU54_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8296941.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12359..12769
                     /locus_tag="LTU54_002246"
     CDS             12359..12769
                     /locus_tag="LTU54_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8296942.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12773..14173
                     /locus_tag="LTU54_002247"
     CDS             12773..14173
                     /locus_tag="LTU54_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HBL8296943.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14196..14570
                     /locus_tag="LTU54_002248"
     CDS             14196..14570
                     /locus_tag="LTU54_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBL8296944.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5233 a         2125 c         3026 g         4673 t
ORIGIN      
        1 attgtttagg gctatactat attggtacaa ggaatagaaa gaggtgttta tgtggttgat
       61 aaaattcagt ttaatcaagt tatagaagaa ttcatcaaag taaataattt aatgacccat
      121 attcaaaaac gaccaatcag aataactaat gatctaaaac tcagtacgag tatgattcat
      181 ttgattgatg tgattggaaa ttatcctgaa actagtatta cggaattagc tgatcgatta
      241 ggcgttacca aaggagctgt atcccaacag attcccactt tagagaaaat gaatcttata
      301 agcatttctc aaaaaaaagg aaataagaaa actaaacttg tatcgttaac aaaatatggt
      361 cgaaaggtgt acgaatcaca taattattta catgaagagc tttatagctc aataaatgaa
      421 gcgttagaat cattctcacc agaccaaatt tcaaccatta aacagattct agaaaaagta
      481 tcttatacaa tcaatgaata tcaagtaagt ctgacaagaa aggaagattg acatgaaaaa
      541 aatgattttt gaatcggcta cactagacta gacagaaaaa ataaggtgtg taaaataaat
      601 gaaaacacac tggaggaatt tttatgtcaa gacgtcaacg tagaacctat tcaaaagaat
      661 tcaaacaaca agtcgtcgat ctttatctcg caggaaagcc ccgagctgaa atcattcgag
      721 attatgagct gactacctcg gcctttgata agtgggtcaa gcaagcgcaa actactggct
      781 cgtttcagga aaaagataac ttgacccctg aacaagtaga actaattgca ttacgaaaga
      841 aaaataaaca gcttgaaatg gagaatgaca ttttaaagca agcggcgctg atattgggac
      901 gaaaagacaa gtaattgata ccaataagca caaatactca atatcagcga tgtgtaaagt
      961 gttaaacatc tcttgccaaa cctattatta tcaagccaaa ccagttgaag atgaatcaga
     1021 actggaagaa ctcgttcaag aagaatttat tcgcaatcga aaagcttatg gtaccaggaa
     1081 gctgaagcag tgcttggcca atctaggtct tcaagtgagt caacgcagaa tcggccgtat
     1141 gatgaaacag cgtggcttgc gttcaacata cacattggct catttcaaat tccattcttc
     1201 tacttgtaac gaagccaaga ccgccaatat tctagacgga accttcctac aggaaaagtc
     1261 attagaagcg attgtaaccg accttaccta tgttcgtgtt gggaagaaat ggcagtatat
     1321 ctgcctgatt cttgatttgt ttaatcgaga aatcatcggc tattcatgcg gggagaagaa
     1381 agatgcgacg ttagtcaagg aagcttttgc aaagattccg tactcgttga ataatgtaca
     1441 actttttcac acggatcgtg gaaaagaatt cgataaccag acgattgacg aaattttatg
     1501 ggcctttaat atcacgcgtt ccttaagcaa aaaaggctgc ccctatgata acgccgttgc
     1561 agaatccacc tataaatctg tcaaagtaga attcgttcat caataccgct ttgagacatt
     1621 ggctaatttg cgattagagc tatttgatta cgtccattgg tggaatacca tccggctgca
     1681 cggcacgtta ggctatgaga caccgatcag ttttcgacag cagagattgg cgcagcgaat
     1741 ccttgataat gagctcggat gtgataccgg tggcgaggca ccttaattat ttgaatgagc
     1801 ttctgcctta gagaatcaga ttcgaggagg ctcgttgtca agggcagtcg gaacaccagc
     1861 agcaagctta cgcaatacct tataattttt gtcaaaaaaa gtgttgccat tccacatata
     1921 tctttaaaca gctattgcca gataaaaata aatgatatta tatatataaa tacttatccg
     1981 aaaaggggaa tgagagagtc aaggttcttg gaatgtcgaa tagtcgagta ggtagatttt
     2041 ctatctacaa ccttgttgtt taaaaatgaa aaaaattgga attagagaag ttgcgaaaga
     2101 ggcaaatgtt tctcctacga ctgtgtcgag ggtattgaat gatcgtggat atttgagtga
     2161 ggaaacaaaa agaaaagttt ttgatgccat gaaaaaatta gaatactatc ctaatgaact
     2221 ggcgagagca ctttttaaaa acgaaacata taccgtaggt ttgatttttc ctacaattac
     2281 taatccattt cacgctgagt taattcaaga tatcgagttc gagctttcca gcaggggata
     2341 taaagtgttg ctgtgcaata gcttaaacaa tccaacaaaa gaaaaagagt acttgacgat
     2401 gttgagaaag aatcaggttg atggaattat agtaggaacg cacaataaag atattgcgga
     2461 ttatgatatt ccagggctac ctattgtagc gattgataga agtcttggtg aaaatacagt
     2521 tacagtatct tgtgataatt acaggggtgg ggaattagct actcaactac ttgtaaatca
     2581 tggctgtcag aatatactgt gtattcgggg agatagtaag ataaagctac ccgcgaatag
     2641 taggacttta gcatataaag atgttttgaa taaattcatg ctggaaccgt tagtcctaga
     2701 agtaccattt gtaaaagaaa tcaatgaaaa aaatgaaata attaagacat atctggaaac
     2761 tcatccaaat attgacgggg tttttgcagg agatgattta ttggcttctt tggcgattaa
     2821 caacttagga agttcgggca aaagagttcc aaaagaggtt aaagtgattg gatttgatgg
     2881 agctcaacaa acattgattt ataatccaga attatcaact attcaacagc ctatagatca
     2941 aatatctaaa gtagcagtaa aaaaattatt gaatatgatt aagggcgaaa aggagacgga
     3001 tgaactttca cttccagtaa agctcataaa aaaaaataca gcataagtta aatcggcgaa
     3061 aaattccttc gtcgatttaa aaatattttt atgaaacggg ttgacatatg aaacgggttg
     3121 acatatatta gttgtgaaga taaaaacctt ttcactggag gaagaaaatg gagaaattat
     3181 taattaaaag caataatcat caactttact accaacctaa ggatgtttgg gtcggagatg
     3241 ttatgcctta cgggaaagaa ggaaaattct ttatttatca tcaacgtgat actagaaatc
     3301 cagttccttt tggagaaccg tttggctggg ctttagcaac tacgaaagat tttgttcatt
     3361 ttgaagactt tggggaatct cttttaagag gggatgacaa tgagagagac cagtttatct
     3421 acgctggttc tgtatttgag gcaaatggaa aggttcacgc attttataca gggtataatc
     3481 gtgaattctt agcgcaggga aagacatctc aagtgttgct tcatgctgag agtgatgatt
     3541 ttgttcattg ggaaaaatca gaaacagcat tggagttaga accacaagaa gggtatgata
     3601 ttcgaaactg gagagatcca tttgtcgttt gggatgatga aaaagaagaa tatttgctaa
     3661 ttttaggggc gcgaaagggt caggataaac atctacaaac cggacgttta gtggaattta
     3721 catcaaaaga tttaactaat tgggaattca aaggtgattt ttgggctccg ggattgtata
     3781 ccatgtttga aatgccagaa cttttcaaga ttggtgactg gtggtatctt gtctattcag
     3841 aatactcaga tggaaataaa atactctatc gaatgagtca aagtttggaa ggtccttgga
     3901 ttaagcctaa agatgaagct tttgatggac gggcttatta tgcaggtaga acagcttatg
     3961 atggaaaacg tcgagtccta tttggatggg taccaacaaa agatcaagaa aatgataaaa
     4021 acaattattt atggggaggt actttggttc ctcatgaagt ctatcaaaaa gaggattttt
     4081 cacttggggt aaaacccata gacgaagtgt ggaatgcatt cataaattta catgatattg
     4141 ccgatgtaga gatagaaaca gcagacacaa gagaagaagt aacgcttgtc aaaaatagtg
     4201 gaactttgtt taatttagaa acaacaattt catttgatga gaatcttagt gacttttcac
     4261 tgagattata taaaaacctt gagacagatg aatcttacga atatcgcttc aatttagatg
     4321 aggggcgtct tacatttgat aaaaaccctt gttacccttg gtttcaatat atggataaag
     4381 gacttattag acctatatct ttaaagccta accaatctta taaactaaaa gtgattgttg
     4441 atgacaatat aatgacactt tatattgatg gaatcgctct taacgctcgt gtacatggtt
     4501 gttttggtca agcaattact attacagcaa caaatggatc aatgactgca ataaatacca
     4561 agttttcaaa tgaaataaag aaataataaa cgtcaatcat gtggcaggtc tgaaaatcaa
     4621 acatgacctg tcacatttaa aagggaggat aaatgaatgg actacaaaaa aattgccgaa
     4681 gatgtattgg aaaacgttgg aggaaaagag aatatctcaa gcgctacaca ctgcattact
     4741 cgtttacgtt taattcttaa agacaaaagc aaagcgaata tggaaatgct tgaggaaata
     4801 gaaggtgcaa aaggtgttct tttcaatagt ggacagttac aagtaatttt cggaacaggt
     4861 ataattgaaa aggtttacaa agagttcgtt gaattgacag gagctaagga agtatccgtt
     4921 tctgaaatga aagacgaagg agtcaataat tcaggaaata gacttcaacg agcttttaaa
     4981 gttttctcgg atatctttat tccgatcatt ccagctttta ttggggcagc aatggtttta
     5041 ggaattcgtt cgttgattac cactcctgga atgtttggaa tggatggctc attcgctgat
     5101 cacaatgtat tcctaaatga tttatccaaa tttctgggta ttattgcaac cacatttgat
     5161 tatttaccag ttttagttat gtattctgca acaaaacgtt ttggcggaaa ccctatttta
     5221 ggattacttg taggatttgt tatgattcat ccaggactag ccaatcggaa tgatttcgtc
     5281 atgggaacta taaagcctga ttattggcat ttatttggat taagcattcc agcagttgcg
     5341 tttcaagggg gtgtttttcc agcaattttg acttcttggt ttttagcaaa agttgagaaa
     5401 attacaaata aatattctcc tcaagtactt gcttacattt tagtcccaac tgtgacaatt
     5461 gttcttgcca actttgcact atttttagtg tttggtccat tggggaattt tatcggtaat
     5521 gttttaggtg gaatcattga tttcctatat atgaagatgg gtgcctttgg tgcttttgtt
     5581 tttgcaggcg cgttacaacc attagttgta acaggaacac aacatgctat tcaaggtatt
     5641 gaggcaaact tagtcgcaac aactggattc aattatatcc aacctatttg gtcagtttca
     5701 attattgccc aaggtggcgg ttgtattggt atgtatttac tctttaagaa aaaatctaaa
     5761 gatagagata ttgcgatttc aagctttatt ccaacactgg taggtattac tgaaccggct
     5821 atctttgctg ttaatttgaa atattcaatc gttccttttg tttgctcgtt tattggtgct
     5881 ggttttggtg gagcatttat gaaaattttt gatgtaaaag ccattggaca aggattgact
     5941 gtattgccag gtctgactat tgctaatcca gttcttcctt atatatttgg taatctcata
     6001 gcattcattt taccaatcat ttttattctc gcttatgata aatataaagt gtttatccct
     6061 gaagctaaac accgtaaatt tgcagaaaaa actgactcaa aaaaattgcc agttcaagta
     6121 aagtgagatg agagcatgtt aaattttttt agaaaaaatg aaataagcgt tgtagcacct
     6181 gcaaccggtg tttttgaaaa actggagaaa ttaagtgatc cagtcttttc aaaaggaatg
     6241 atgggacaag gatttgcaat tgatgcaaaa aacgaaacga ttgtatctcc aatcgatggt
     6301 tttgtttcaa gtatttttcc tacaaaacat gccttaggaa taaaaacaaa atcaggaatt
     6361 gagatacttg ttcatgtagg aatagataca gttgagctaa atggtgaggg atttgatatt
     6421 aaagttcaag aaggacaaaa agttaaggct ggggatattc ttatgaccat taattttgta
     6481 gtttttgaaa agaatcacaa gactaaagat gtcattgttg tttttccaga ctaccaagac
     6541 gaaattttca ttccgaaact tgaagaagta aaacaaggac aagaattgtt tgttcttggc
     6601 taatgataag taattaataa tagaatcgag gaaaatgtat ggtttttggg gctattgaag
     6661 ctggtggaac aaaatttgtt tgtgccattg gagataaaga tttgaacata tttgaaaaag
     6721 tcagctttcc cacgactaca cccaaagaaa caatgaattt agttttagat ttttttaatc
     6781 atcataaaga tgatttagaa ggtatcggag tgggatcgtt cggtcctatt gatattcatt
     6841 gtgaatctaa aacgtatggc ttcattacat caacgccaaa acttgcttgg caggattttg
     6901 attttattgg aagtttaaaa aagcaactta atattccaac tgcttggacg actgatgtaa
     6961 atgcctcttg ctatggtgaa tatgtttctg gaaatggcag aggcctttca agtgttgttt
     7021 actataccgt aggaacgggc ataggaggtg gcgctatcca aaacggaaac ttcattgaag
     7081 gttttagtca tccagaaatg ggacatatgc ttgtaaaacg acacaataaa gacaactttt
     7141 ctgggacatg tccattccac ggagactgtt tagagggctt agctgctggt ccggcaatcg
     7201 aaagaagatc agggaaaaag ggacaagaag tgactgaaaa tgacgttact tgggaaatag
     7261 aagcttatta cattgctcag tgtgcatata atacaacatt gatgttatca cctgatgtca
     7321 ttatatttgg tggcggagta atgaaacaaa aacacttaaa aaagaaagta caagataagt
     7381 ttattgagtt gatcaacgga tatgtcgcta ttcccgatgt tgacaattat attatgacac
     7441 caaaattgtt agataattct ggtattgtag gttgtttagc tttagcaaga gacgttaaag
     7501 aaaaatctta atttggaaaa aagatcgtct cgactatttg tttggttgat gcgatctttt
     7561 ttttcaatta gtaattgaag ggacgcctaa aagtgaaaaa aaaaatgaaa gatgaagtat
     7621 tagagtatat caaaaataat ttaaagtatt acgatttttc tgctcaagat attgcaatga
     7681 aattttgtat taaaagaaat gtcgcttccc actatttgaa tcagttattt tcagatggaa
     7741 aattactaaa gaatgattct gttagaccag taatgttcaa gtataatcaa caaaagccaa
     7801 aagactgctt ttctaagttt attggagcag acatatcttt gaaatccact atagataaat
     7861 gcaaagctac tgtaatgtat ccccctaatg gcctcccatt aataataaaa ggaaacagtg
     7921 gcgttggaaa aagtttttta gcaagtttga tctatcagta tgctcttgat agaaaagtaa
     7981 ttcataatga cgcaaaattt gttgttgtga attgtgcaga ttatgcaaat aatccagagc
     8041 tattgtcagc agttttattt ggatataaaa aaggagcctt tactggggct gaaaaagata
     8101 ctgcagggat gctatcttct gctaatggag gatacctttt tttagatgag gttcacaatc
     8161 taagtgcaga aaaccaagag aaattgtttc ttcttatgga ttctcaaaag tatcgtaaac
     8221 ttggtgaaag tgtgcaatgg gagtatgcaa atgttcgcct tatcttagcc acaacagaag
     8281 ataaaaatac ctctttgtta gcaactttta gaagacgcat cccagctgaa atcactctgc
     8341 cagattacaa taatagaagt acatctgaaa aaattcaatt attatttgaa tttcttaaag
     8401 atgaagcaat tacaattgat tcaaaaatat tttgttcgat tgaacttttt actgatcttt
     8461 tatcgaaaac atttgaagga aatgttggag aattgaggaa tgaaatcaag cttctttgtg
     8521 cagaaggtta tttaggaaat aagagaaagt cttcaattta tttaggggac aaactagaca
     8581 gcggtttttg gatagatcca gaaattagta tagatttaaa aaaaatgttt cttaaatcac
     8641 tttctgaaat tgatttgatt gaactttttc aaaatcacat ttcaattgaa gaaagcatga
     8701 gtcaattaag agatagaata cttaaagagt gtgcaggttc ttctgagtat aattatttag
     8761 aaagtgacga atttatagca ttaagaaact atgttgttaa taaaatatca ccaattattg
     8821 attcgacagg tctatgttta ttagatgaat tcttggcgga tatcagtcta tttataatgt
     8881 ttattgatgt aattgaagaa acatcaagaa attttttgct gtctaagttc agaaaatttc
     8941 gtatgaaaaa tgataaacaa aaattattgg caaatgagat ttggagtagt ttagaagtcg
     9001 aagaaagtaa acaagaactt ttgttgaaat ggatactttt tcttgtaaag aagttctatg
     9061 tgaaaatacc agaaacccat tgtctgatag ttatgcacgg taaaataaca gcttctgcga
     9121 tcgcaaggga aacaaataag ttgctgaata catatgttta tgaggctttc gatatgccaa
     9181 ttgaaggtga gactagtgac ttaattaacc taatcaacaa tttttgtaaa agtatagata
     9241 cgacgaatgg actcattctg ctagttgata tgggatcatt ggaacaaatg tatgaaaaaa
     9301 ttgaatccaa tgtaataggt gatttggtaa ttttaaacaa tgtttctaca gctttagcga
     9361 ttgaatgtgg gattcaagtt tgccaaaaaa agccaattag tcatttttac cagatggatt
     9421 ttgattcgtt tcaagttaaa gttcagtatt ataaaggatt atcacaaaag aaaaatgtaa
     9481 tagtatcttg tctctctggt gaggggattt ctgaaaaagt taaggatatt ttaaaacggt
     9541 atctcgataa tcaagtggag attttgattt ttgattttaa cgcattaaag aaaatagcaa
     9601 aggaaaaaga tactatgatt tttaagaata caatttgtgt tctttcaacc actgaatttt
     9661 acattcaagg aatcgattgt ttaaatttag aaaatctaat taatgggaat caaaccttag
     9721 aaaaattaaa taagtatatg gactcggata gctgtgagtt ctgcttaaat gaattagtta
     9781 aactctttac tattgaaggc gctagtacta agcttcgctt tttagattcc aaaaaagtat
     9841 tcaatgagat agaaaaagta ctatatttgt atgaaaaata ttacgatgtc aaaatcccaa
     9901 gttttctgag aattaatcta tttctacatc tttctggaat gattgaacga attatgattg
     9961 gtgatggaat aacaaatgaa catttgaaag agggagcgca actatttgat acttttttaa
    10021 atgtatcaaa agattttttt tcagaaatca aagatattta ctgtattgaa atacccaaag
    10081 gagagtatga gttaatctat acaatttttg agcagactat tttctaaata gcatactagc
    10141 gtctaaaaaa atgaaagggt gtccaaagta tatttagacg ctcatttaat taatatttac
    10201 tgtattttcc cgctttattg gataatgtta gttggcacag taattgctta tataaaagta
    10261 taagaagaaa ggagagaaca aaatggcgtt ttggcaaaca tttttagttg tgttgtatgg
    10321 atttttcatt aactgggata aggaagggcc caaaatcgga acctcccaac ctgtcgttgc
    10381 aggtttcttg accggactta ttatgggaga tattcaaact ggactttata tcggtgggac
    10441 tttgcaatta atgacactag gtattactag ttttggtggt gcatcagtac cggattatca
    10501 aacagcagca ttgatcggta cttatttagc aatctcaact aaccaggatg cttctatagg
    10561 aataacttta gcaataccgg tagcaatgtt aattgtacaa ctagatgttc taaagtggtc
    10621 aacaaacatt ttttttcaac aaaaggcaga aaaattcgca caaaaagaag attatcaaaa
    10681 attagagtgg atgcaatatt tggcatcaat taatacaagc tttgtatctg gaattcctgt
    10741 tttgttaact gtgatattag gcccaacgtt agtgggaagt gcaatcaatt atattcctga
    10801 atgggttacc ggtggtttaa aggtagcagg gggactatta cctgctgttg ggataggaat
    10861 gctacttaaa tatttaccta caaaagatta ctttgcctat ttgttagttg gtttcgtttt
    10921 ggcagtttat ttccaaatac caattttagg gatttcgtta cttggcgctg caatcgcact
    10981 aatcgtatat aaaaatagat caaataatac tcaagtagca ccagctgcaa caggaggttt
    11041 tgacgaagat gaataagaaa ctgacaaaaa tcgacaaaca agtaattttc cggtggattc
    11101 tcaatggtgc aacttcatta aattatgaaa aaatggaagg gttgggttat gcgtattcta
    11161 tgcttccttt tatcaaggac acatataaag atgatcctga aggaatgaaa caatccgttt
    11221 tagctcacct acaatttttt aacactacgc cgtatacagc gccttacatt attggtatga
    11281 atgttggaat tgaaaaagaa gaaaaaaata agtctttgga agcagtaagt gctctgaaaa
    11341 caggtctaat gggtccttta gcgggaattg gagatagtgt atttgtagtt attccatgga
    11401 ctatttttgg agcaattgca gctaatatgg cattggaagg tagttttgtt ggaattattt
    11461 tgtggatact ggcatcaatt ttattaagga ttactgctta tcctttatac cttgctggga
    11521 aagaatcggg tgcgagccta ctaactcaaa ttgaaacaaa attaaagtct attactgatg
    11581 cagtatcaat tttagggtta atggtggtcg gtgcactcat tgccacagtt gtaaaagtga
    11641 atgttggctt tgaatttaaa caaggcgact taacaatgac cggaaatgaa atctttgatc
    11701 aagttatgcc tggtttatta cctgcatgtg tagtagcaat tgtgtattat ttgttaggac
    11761 gaaaggttaa accagtttat gtaattctat taatcttagt agtttctatt gcgttaagag
    11821 cgttaaatat tttgaagtag gtgttgaata tggatgaaaa aatgaaaggt attatacatg
    11881 ttcgtattga tgatcgaatg attcatggac aagtagcaac acaatggaca aatcaattga
    11941 gtgctaacag aattatggtt attaataatg aagtagccaa tgatgacgta aaaaaagctg
    12001 tagttcgcct cgctgcacca cctaatgtta gaacttcaat aattacaagg gaagtagcag
    12061 ttaaaaatat tctttctggt aaatatgaag gacaaaaagt tcttattgtt gcagtttctc
    12121 caatcgatgt taaatttttg attgataacg gtttaccaat aacaagtgta aacgtaggaa
    12181 atttatcaag aaggaatgga acagaacgca tccgtccgac gataaatatt actgaagaag
    12241 agagagaggc ttttaaagaa ctaattagtg atggagttga agtgacagtg atacaaactc
    12301 ctagagattc aaaagaattt ttaaaggatt ttatttatta agaacagagg atgagtggat
    12361 ggagtttata attacatctc atggaaatat tgccattgga tttgtagata cattaaaaat
    12421 gttttttggc gataatttaa attgtcatgt ggtaacactt ggagatgcag gcattgaaga
    12481 attccgtgaa aacattgaaa aaatagtttc tcaagtaaaa aatcaagaag ttatggtatt
    12541 tgctgacttg attggtggaa cacctttcaa cgaatttgct aaaagaagtg aagtatttca
    12601 aaatgggttt caacttcttg gtggcatgaa tttaggaatt ttgattgagg ctgtcaattt
    12661 gagattacaa ggaaaaaaaa gcgatgatat catcgaaaat ctaagaaata tgaatacaat
    12721 tgcgtgtctt gcagatatgc aaagacatac taacgaagag gatgagtgat aaatgcaaaa
    12781 aattttttac aagcctaaag atttttggtt tggggattgc atgccttttt ataaagatgg
    12841 aactttttat ctatttcatc aacgagatac gagaaatcct aaacctttaa ctgatccttt
    12901 tgggtggtca ctagttacaa ccactgattt tgtgaaattt gaagaatacg gggaagtaat
    12961 tcatcgtggt ggcaacgatg acattgatca atacatttac gctggaagta ttgtccaagg
    13021 aatcaatgag tatttagctt tgtatactgg acataatcgc aaagctaagt tggatggaaa
    13081 aacttcagaa gtgttaatga ttgcaagttc taaagattta attcattgga aaaaggatgg
    13141 cgaagcagca aaacttgttc ctcaagaagg atatgataag aatgattggc gcgatccttt
    13201 tgcaattttt gatcaagatc atggaaaata cgttttgata ctaggagcta gaaaaccagc
    13261 aagtaagaaa aagccgacgg ggagattggt ctattttgaa tccgaagatt taaaagattg
    13321 gaatttcaaa ggagactact gggctaataa tgaatttaac atgattgaaa tgcctgatat
    13381 atttgaaatt gatggaacgt ggtatttact tttttcagag tattgtgaag ataaaaagac
    13441 taaatatcga gtcggagcag gattattttc agattggaaa tcaccagtag acgaagcttt
    13501 cgacggaaaa gcctattatg cagcaagaac agtcggaact aatgaaactg gccgttttct
    13561 aactggctgg gtggcaacaa aagaagggga gaacgatcaa aataactggg attggggcgg
    13621 tgcacttgtt ccacatcaaa ttattcaaaa gacagacaaa actcttggtg ttaaactgcc
    13681 tgaaaaaata attgagaatt ttaaagaaat cactccattt tcaagtttta agttagagaa
    13741 agaatttgga aaaaaagaaa gagtaattgt tcctaaaatc gaagattatt ttctatttga
    13801 aacaactttg actattacat cgttaacaaa ggagtttgct ttaaaattat ttgtagatag
    13861 tgatactcaa gaaggatacg aatttcgtat ttcattgatt aataatcagc ttactttcgg
    13921 aaaaactccg aactatcctt ggcctcaaat gtttgataag gggctgcaac gaccactatt
    13981 tttggaaata ggcaaagaat atgaactaaa gttaattgtt gatgatacga tagctgtttt
    14041 atatattgat aatgttgctt taagtactag aatgtatgat aaagtcggaa cagcattaag
    14101 tatgactgtt acggagggag caatcaaggt agataaacta gactattcaa taaattatga
    14161 atgtggattt taaaacaatt gaagggatga tgtaagtgaa attaaatggt ttacaacata
    14221 ttggaattcc aacaaaagat tttggagctt caaaaatctt ttatgaatcc ttagggttta
    14281 gtctgattaa tgaagaaatg aatgtgggaa gtaaagtcgg ctttttcgag ttgaatggaa
    14341 cgataataga aatatgggaa gataaaacta ctgagtgtag aggagccatt gatcatctag
    14401 cattagatac ggatgacata gatgaggtat tcaattcaat tcaagattta ggatatgaat
    14461 tattagataa aaatattatg gaattaccgt tctgggaaaa tggaatccgt tactttaatt
    14521 tctacggacc aaatcatgag attatagaga taagccaaat gaagaaataa atagataacg
    14581 aagtgctcta agttggtatg aacgaaaagt acttcatttt ttggtatcag catttcaata
    14641 gtttaatgag aatttactat ttcaaattaa ttcaaagtga cagtattttt tacttatttg
    14701 gtttcaaatt ttgtaatcct tcctatttat tggaatacac tccacatttt gtatacaagt
    14761 catatcatgt tttagcaaag ctcttaccaa gcaaagttat aatgatgttt tccaaagttt
    14821 gatctttcga tggaggctaa cacatatttt ttgcaggaca gccaaaatta gatgtataga
    14881 atagagtctg tgaggcgtat agacagttta ttgtctgtat gccttttttt attttgaaaa
    14941 atttttagta tttttcgaaa aatgctcact cctagagtaa gcgccccata gaacagtacc
    15001 ttactaacaa atgcacctca agatatactt gagctatcaa gaagtctgga ggttttc
//
LOCUS       DAFGZE010000068        14863 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.070, whole genome shotgun sequence.
ACCESSION   DAFGZE010000068 DAFGZE010000000
VERSION     DAFGZE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.070"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            441..1055
                     /locus_tag="LTU54_002249"
     CDS             441..1055
                     /locus_tag="LTU54_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8296945.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1828..3567
                     /gene="yfcC"
                     /locus_tag="LTU54_002250"
     CDS             1828..3567
                     /gene="yfcC"
                     /locus_tag="LTU54_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBL8296946.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3724..4908
                     /gene="iadA"
                     /locus_tag="LTU54_002251"
     CDS             3724..4908
                     /gene="iadA"
                     /locus_tag="LTU54_002251"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBL8296947.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5019..5222
                     /locus_tag="LTU54_002252"
     CDS             5019..5222
                     /locus_tag="LTU54_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBL8296948.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5323..5985)
                     /locus_tag="LTU54_002253"
     CDS             complement(5323..5985)
                     /locus_tag="LTU54_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8296949.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5998..7500)
                     /locus_tag="LTU54_002254"
     CDS             complement(5998..7500)
                     /locus_tag="LTU54_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8296950.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVTVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8048..9727
                     /locus_tag="LTU54_002255"
     CDS             8048..9727
                     /locus_tag="LTU54_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL8296951.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9854..10333
                     /locus_tag="LTU54_002256"
     CDS             9854..10333
                     /locus_tag="LTU54_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HBL8296952.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10552..11232
                     /locus_tag="LTU54_002257"
     CDS             10552..11232
                     /locus_tag="LTU54_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBL8296953.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11240..12571
                     /locus_tag="LTU54_002258"
     CDS             11240..12571
                     /locus_tag="LTU54_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL8296954.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12586..13548
                     /locus_tag="LTU54_002259"
     CDS             12586..13548
                     /locus_tag="LTU54_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBL8296955.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13659..13997
                     /locus_tag="LTU54_002260"
     CDS             13659..13997
                     /locus_tag="LTU54_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBL8296956.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14076..14783
                     /locus_tag="LTU54_002261"
     CDS             14076..14783
                     /locus_tag="LTU54_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBL8296957.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4773 a         2580 c         3193 g         4317 t
ORIGIN      
        1 tatatatata aatcatacca atgttttttt gtattttctt gcttgcactt ggataagaga
       61 ttacttacaa taaattcaga tagaaacagc tgaataaaaa aaggctccct gacccttagt
      121 tggcgctaag aatcagggaa tgaccagtca cacacataca ccctgaccga tcattttgcc
      181 atagtcaatg cagtgcatcg acctaattta ttgtacctaa tctagggtga aaattctacc
      241 cctatgtaga tattttccat ggtctgcacg ttgttttatt tgctatgtca aggtattggg
      301 cgtattcgtt caatgccttt tttgtgtttg aaacagcaaa gaccacatgt gttactgtct
      361 aactactgtt tttttatgta cttccttaga ttaggcagct gtttctattc atgtcaagta
      421 tagctaggcg ggagagataa atgagtttat gttattttga ctattatcac caattggaag
      481 agaaattaag gatagaagag ccgctgatgc ttcaagctgg cacttggatc gcgaaacaaa
      541 ttaatcaagg tggtagattg cagatatatg ctagccgcac actaagtggt gtagcatttg
      601 aattttggga tcaaaccccg gatcttgtac caagtatatt gattgaaaat ccagccggag
      661 gtgtctatga aacgctagaa ggtgcaggac aggcaattat tgatcagctt tctgtcaaat
      721 cagaagacat ttttcttctt ctttcgaatg aaggaagaaa cccggcaatc atcgaactag
      781 ctcagtggat caaagaaaat ggtcatccat taatcattgt tactggattt gatttgtcac
      841 gaagtatcaa atcgcaacat agcagtggtt tgcgattatt tgaatttgct gatttggttt
      901 tggacaacca cgccaattta catgatgcag tcttgacttt gccaaatcta gatccagcga
      961 tctgcggaac agcctctctt gcaacgatct ttttgctgca gcaagttttg tattttacag
     1021 caaaacagct agtccaggac acaagtggaa aatagttatc cacatgttga caaagttcct
     1081 taaaaaaata atgttccaca taaaaatgga gcgtttgaaa aaaagaaaac gattattaat
     1141 gaggattaac aacgactact ttttttgttt tagttatttt aagatggatc atctttgatt
     1201 ttaagcttgt tttgtttaaa gtttgttatt tttataagtt gtttcacagt tatgagaaat
     1261 gagttataag aatatgtagt gtcagaaaaa taaaaagtag tggaaaaaga gacaagaaag
     1321 aatccatatg gattctttct tgtctcttaa tacacgttga tgaaagagtc ttccccaaaa
     1381 ctctttacaa agtaattata gtacatttgt tatcattatt gcaatgaagt ttacctgata
     1441 taacaaaatt gtccaacagg ataccttttt ctaatagaaa taaaatacac aaaaattgaa
     1501 ggatagataa tggatattta taagtgttta ttattttttt tgcaatgaaa ttcaattttt
     1561 caatgattgt aaaatgaaaa aaacaggacg agattttctc taatctaaaa ataaaaaaag
     1621 aattttaagg aaaaacagag aaataaaata cttaatataa atggaaagag aaaaaaataa
     1681 tcatacatag gagaaaaaaa acaaaataga aaaaagtgat tttctaataa attattcatg
     1741 tggaaatgtc atttttactg tataattcat ttgattatcc attttttatt catcaaaatt
     1801 actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa attcttcagg aaaaccgaaa
     1861 cgcgacttca ctaaattatc gacaccccat acgtatgtca ttatttttgg tgtagtcatc
     1921 tttgcatgga tactgacttt tgtcgttcca gcaggaaagt tcagcacgca agatattgaa
     1981 tacaaagatg caaatggcga aacaagtact cgtactgttt tacgtcaaga ttccttccgt
     2041 tatgcttacg agctggataa gtcgtatgtg tttgatcaat tagaagaact gcaagatcat
     2101 ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat tggaaaaagt cattgctgac
     2161 ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct ccttgacaga tgatgtgctt
     2221 tacgatcagt acggcgaaaa tatctatgat acttctaaaa aattgcataa aacagccaaa
     2281 atatggggaa cagatgattt tggcggcttt ggtttcttaa attttgtttt tgaaggacta
     2341 gtatctggag ataagtatgg atctgctgta gggatcgctg cattgatcct tgttgttgga
     2401 ggtgcttttg gtattattat gcgtaccgga gcgatcgacg caggtatcta tgcgtttatc
     2461 agtaaaacta agggactgga gcgtttagct ttaccgctat tattctttgc tttttcattc
     2521 ggtggcgcta cattcgggat ggcagaagag gtaattccat tctcaatggt gatggttcca
     2581 tttgtcattg cgttaggata tgactcgatc gtagcagtaa ccgtgactta tgtcgcatct
     2641 caagtcggaa atgcgacctc ttggatgagc ccgtttagcg tagcggtggc tcaagggatt
     2701 gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga ttatgtgggt agttgttact
     2761 gcattagccg ctggttatat gatgatatat gcagaaaaaa tccgtaaaaa accagaaagt
     2821 tcgttgactt acaaatcaga tgattatttc cgttctcata tcaaaaagac atctgatgaa
     2881 aataaaaaat tcatgcttgg gcataaattg atcttattag agatgttagt tgtccttgtc
     2941 tggatcgttt ggggcgtgac tcaaaaaggc tattacattc cagaaatcgc ttctcaattc
     3001 tttgtgatgg gattagttgc tggaatcatt gctgttcttt tcaaattaga tggaatgaaa
     3061 gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg atttagctgg tacagcaatc
     3121 gtagtaggta tggccaaagg gattcttctt gtcttgggag gatctgatgc atctgttcct
     3181 tcagctttga atacgatttt gcatgggatc gggacggcgt tgaccggtgt tccagctgtc
     3241 attggtgctt gggcgatgta catcttccaa agtttgttca acttggttgt tacatcaaat
     3301 tcaggacaag cagctttgac tatgccaatc atggctccac tggcagattt agtaggtgta
     3361 tccagacaag tagcagttct tgcttatcag cttggttcag gttttatgga cgcttttaca
     3421 cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac ggatcgaatg ggcaaaatgg
     3481 gcaagattcc aaatcaagat gcaaggattc ctgtttgttt taggaactat tttcatcatg
     3541 attgccattg caataggtct gcagtagtac tggagacctg taaataaaaa agtaatccaa
     3601 acagtagata aggactccaa gaaccgtcat tttacggtaa aatcaaagga gtaaagatcc
     3661 tatcgtttgg attatttttt aagatcctat catcagttat acttaaagaa agaggaaaaa
     3721 tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag attacttagg aaaaatggat
     3781 gtactgattg ccggtgggaa gattttggct gtagaagaac agctagaagg cggctacgaa
     3841 ggagtggaag tagaagaact ttcaggtgag ggaaaagtac taaccccggg tttcattgac
     3901 tgtcatttcc atattttagg tggaggagga gaaggcggtt atcaaaatcg aacgccagaa
     3961 gtcacattga gccaattgac tacagctggt gtaacgacag ttgtcggttg tttgggaaca
     4021 gacggagaag gtagagatat gactgcgttg atcagcaaag cgaaaggtct ggaagcagaa
     4081 ggaatctcca cttatgtata cgaaggttcg taccgattgc cggtaaaaac agtaacagat
     4141 tctatcatca aagacttttt gacaattgat aaaattatcg gaatcggaga aatcgcagta
     4201 tccgatcacc gttcttctca gccaagtttt gaagagttcg ctcgtgcagt agcagatgct
     4261 cgtgtaggag gcatgttatc cggaaaagct gggatcatca atgtccactt aggcggagga
     4321 aaaagaaagc tagagctatt gacacgaatc gtagaagaaa cagaaattcc gatcacacaa
     4381 tttttgccta ctcatgcaaa tcgcacacca gaattatttg aagcttgcgt ggcatttgcc
     4441 aaacgtggtg gcacgattga cttcactgct agcgaagatc ctgatttttg ggagaagaca
     4501 gatggtgaag ttcgcttcag caaagcactg aaacgattga tcgaggaaga tgtttcttta
     4561 gataatttca cgatgacctc agatggacaa ggaagcttgc cttattttga tgaaaacaat
     4621 catttcctag ggttaggagt aggaagtgcc aaggcattgc tagtagggat caaagaagct
     4681 gtgcaaaaag aaagtatccc tttggaaatc gccttacgag ctataaccag taatccagca
     4741 cgtatattga aattggataa aaaaggaaaa atcgaaattg gtgctgatgc agatctgtgt
     4801 atcttggata aagaaacgct tgatattgat acagttatcg ctaaaggaga aataatggtc
     4861 caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa gtttttaatc aacagctcta
     4921 actatctttt ttagaaaacg agcgttctgt ttgtctgtat ctgttgttga tgttaccctg
     4981 aaggtagatc aataaacaaa ctaagaggag gaagcacaat ggcagattta aaaggacgtt
     5041 ctaaagatat gaaagataaa gtagtcggtg aaggaaaaga agcatacggc aaagcaacag
     5101 acgacaagag caaagaaaca gaagggaaag cacaatcact aggtgctgac atcaaagaaa
     5161 aagctcgaaa tttaggcgac gaagtcaaag acaagttttc tcgaaaagac gaaaaaaaat
     5221 aaaaaccact tattactaat tgaatcaagc aaataaaaaa tccgaaccat atctgcgaga
     5281 cagatatggt tcggattttt tatttgatca ttatcctgtt ttttaaactt cgattttttg
     5341 aaatttttta ttttttagtt caaagacttc atcacagctt tttgctactt caatctcatg
     5401 agtcacgatg ataatacagc gattttgttc atgggcgatt tcttggaaaa gtttgacgat
     5461 atcttgtgaa gttgtttcat ctagatttcc agtaggctca tccgctacga ttagttgatg
     5521 ttcacagcag atagcacgaa cgatagcgac tcgctgctgc tgacctcccg aaagtttggt
     5581 cacctgtttt tttgctaata tttcatcaat tcctacacga gccagtcgat ctaaagcaac
     5641 ttgtcgataa ttctcttgct tggtcttagt gatttccatt gctgctatga cgttgtctag
     5701 agccgataaa taaggaagca agttgtaagc ttggaaaata atcgagacat catttttacg
     5761 ataacttctt aacccgattt tgttcagtga acgatcttga tagtatacct ttccttcttt
     5821 tggtgcatct agtccagaaa taagcgaaag aaaagtcgtt tttccagaac cacttgctcc
     5881 taaaatagcg tacatttttc cagcttcgaa attcagttct acatctttat aaagcgcatc
     5941 ttgcggatct tgataccaaa atcccaattg ttctgtccgt aacatcaaat tcctccttta
     6001 gtttaatagg atttttcttg ggtttaatct taatatccct atagaagcta aaaagacaga
     6061 aaacagactg attccaaatg ctactccggc taatagtccc aattgtgcag gtttgacatt
     6121 gatttccaat tcgctgactt gatcagaaac agcaaatgga ttattcgtgg ttccttgttg
     6181 accatcgcgt tgaccgcctt gttcttgtct gttacctggc atttgtccac cgccaggagc
     6241 ttgctggttt tgtgtcgtgg tttctgtctg ttggtcaagt agctgctgtc ccacggcatt
     6301 cgcgacgacg tttccgctaa gcgaagcaac tcctaatgca agaatcatac aaatagccac
     6361 ttcggtaaac aattggagaa tgattttact tctggattct cctaaagata gcaagacgcc
     6421 taactcatgc cgacgctccc gaatggtaat cataatgatc agagtaagta tgataatacc
     6481 ggctacagtc actagtaaaa caacattttt agaaaaactt gccacgttgt ttaaaggttc
     6541 caacattgac tgatacatgg aatcatttga ttgtaagcta aatgtatctg tgtcgatcag
     6601 cttctcagct tctttgagaa actcatccat tttatctgga tcagatagag tgtaaatagc
     6661 agaatctatc gtgttatcgt cagatgtccc atttagttca tttgcaaaag tgtaagaagt
     6721 gtaaagtgta ttcgacggat tcatgaagtt gaatttcata cccatactgc cgcttgtttc
     6781 gctggattca taaattcctt tgatcgtcat ttcataagtg gtatcttcat cttcagtact
     6841 cgtgattgtg aaagtatctc cgactgccag gtcatttgtt tctgctagag tagaatcaat
     6901 gactacattg tttgtgcctt tgtcgctgtc atctattcct tcaccatccg tgatttttgc
     6961 tgttccatca gaaaaattac tattttgtgc agttgctgac acaccactga tttgaaagtc
     7021 tgcttgagtc atttgcggat tcccgcccat tcctccagca ggttcaccgc ctgcatcagt
     7081 agtttctgaa ctgtcttcac ttgaatcaga actagtgatg gcagttattc catcttttgc
     7141 catagctgag gcggaacttt caaaggaata actctttaca ttatctaatt cagctatttt
     7201 ttccgcgtcc gttaaggaaa ctggtgtaag agaaaaactg cctggatctg gacgttcttc
     7261 agtggtagaa ccgcttgaag aagtttgttc ctgtttttta aatgaagctt ctcgattggc
     7321 acttaaagtc actgttgctc caacttcttt ttgtgcttga ttcgctgctt cttctgcagc
     7381 gcttcgaatc gtgaggccag ctagcacaaa aataagaatg gctgtgaaca cggcaaacaa
     7441 taacaatgaa cgtccccatt ttacttttgt acttttccat gcacgtttca taaaattcat
     7501 attttgcatc cctcctagta gatgcatttg ataatagaca acctatctta aagctagctt
     7561 aaatcttatg ctgtttgcac accatcaaca tgaggttctg tatatataaa aagtcactat
     7621 agttattgca aaaaatgtgg taaaatgaaa aaagtatgtt tcaaaccgac gaagaagaaa
     7681 tgaaagagga atagcaaaag aaatcctaga tgctacagag ggatttttac gtgcatagtt
     7741 gttctaacaa ttgctgatac tagcgattta gtttatttgt tgtataataa tggggaacag
     7801 ctgaatactt atataagtat tctggctata gcgtttcgca aaagatccat tttgttaatg
     7861 aagtatatcc aaaataaaat gcccatccaa ctgtcagaac gcggatgtgc tggtaatttt
     7921 cctgctgatt ctccataagt tgagaaatag tgattttgca tatgcttcat tggaatggct
     7981 catatattta gataaagaag aaaatcgaca tctttaaact tggatgtcat aagaagaggt
     8041 gaagtttgtg agtaccatta aaattatccc attaggcggc gttcgtgaaa atggaaaaaa
     8101 catgtatgtc gcagaagtgg gcgaggatat ttttatttta gattgtgggc taaaatatcc
     8161 tgaaaatgag ttgttgggga tcgacgtcgt aatccctgat tttacgtatt tagaagaaaa
     8221 cattgatcgt gtagctggtg tctttttaac acatggacat gcagatgcga tcggggcact
     8281 tccttacttg ttgtcaaaaa tttctgtacc agtattcggg acagagttga cgatcgagtt
     8341 agcaaaattg aatgtcagtc gtaatgaagc agccaaagga tttaaggatt tccatgtggt
     8401 agatgagcat acagaaatag actttggtga aacagtagtg agctttttcc gtaccactca
     8461 tacgatacca gattcagtgg ggatcaatct gaaaacaaaa gaaggtagta tcgtttatac
     8521 gggagatttt aaatttgacc aaacggctat cccaatgtat caaacagatt atgcacgatt
     8581 ggcggaaatc ggcaaagaag gagtcttagc cttactaagt gaatcggcaa atgcagaaaa
     8641 tcctcagcct gtagcttctg aagcccaaat agcagatgaa gtatttgata cgatcaaata
     8701 ttgggaaggg cgtatcatcg tcgcttgtgt agcaagtaat ttgcaacgag tgcaacaagt
     8761 attggatgca gcagaaaaag cacaacgaaa agttgtcttg actggtcaag atttcgaacg
     8821 aattattcgc acagcgatgc gtttagaaaa acttcaatta ccaagtgaag atctgcttgt
     8881 tacgttgaaa gacatgaaaa actatgatcc agaagaactg atcattttag aaactggccg
     8941 aatgggtgaa cccatcaagt ctctgcaaaa aatggcaaac ggtacacacc gtaatgtcaa
     9001 gattgaagaa ggagatttgg tttatatcac tactacgcca agcatcgcga tggaaaccat
     9061 cgtggcgaaa acggaagata tcatttatcg agctggtgga acagtgaagt tgatctctga
     9121 gaatatgcgc gtttctggcc atgccaatcc taatgacttg caattaatga tcaatttgat
     9181 gaaaccaaca tattttgtgc cagttcaagg agagtatcgt gaattagctg ctcatgcaga
     9241 tttggctcat gcagtaggga tgccatacaa aaatatctat attactggac gcggagacgt
     9301 gctggaatat aagaacaagc gtatgagtgt tgcaggaact acaagtgcag aaaatgtaat
     9361 gattgatgga ttaggcatag gtgatatcgg aaacattgtt ttacgtgatc gaaaaatctt
     9421 gtcagaagac gggattttcg tagcggttgt cactatcagt cggcgagaaa agaaaatcat
     9481 ttcaaaacct caaattactt cacggggatt cgtttacgta aaagctagcc gtgatctgat
     9541 gaaagaaagc agcaatatga ttgaaacaat cgttgagaaa catttagaaa gcaatgattt
     9601 tgagtggagc aaattaaagc aagacatccg agatcaatta ggccgttact tatttgaaca
     9661 aacaaaacgc cgcccagtga tcttgccagt cattatggaa gctactcaac gtcgaaataa
     9721 aaaataaaca aaaagagtaa gatgaacctc tctatagagt gctcgtcttg ctcttttttt
     9781 gcgaaaaaca aataattttt gaatacagca tccctcgctt tataataaac gatagaataa
     9841 agggagtgaa tcaatgatga gtcaaacaaa agtcgaacgt tatttagaag aacaaaagat
     9901 cagctatcaa ccacttgatt ttagtcatgt ggtagaagaa tctttcacag aagaactaca
     9961 aaaaagaggg attgatccag ctttgatatg taaaactctc gtagtgaaag gaaacaaaac
    10021 tggggtgatc atcgcattga ttcctttgaa cgatcactta gattacaaaa aaacgagaca
    10081 agtgacgaaa gatcgtaaag ttggttttcc tggaatggat tttgtattaa tgcatacagg
    10141 atatccccac ggagcaaaca cccctattgg aataaaactc gcacatcctg attattgttt
    10201 tttagcagac agttctttaa aagaaaaaga agaagtaatt gtgtcaagcg gtgaaatcgg
    10261 gagaagcctt cggataaaag tagccgattt agaaaagatc gtccatccta tttacgctga
    10321 tttaattagt taagggacga gaagagaagc taagcagagc agagacctca aacttgagat
    10381 tatatctgct tggtacttgg tgcatcctgt attatctgtt acaattgctt tgtacatact
    10441 gtactatcaa tccaatgaaa atatatttag cagctatatt ttttcattga tgtgcaagtg
    10501 atgaaatcgt ttttaataaa aaagatacag agaaaacgcg aggtgtaaaa tatgaaaatt
    10561 acgtatcatg gacattcctg tttaacaatt gaaatggaaa atggccaacg cgtgatgatt
    10621 gacccgttta ttacaggaaa tccattgaca gacttaactg cagaagaagt aaagacagat
    10681 tggttgctga ttactcatgg tcatagtgac cacataggag atatgattcc tattgcaaaa
    10741 gaaaatcaag ccacaattat cagtattgtt gagattgctg attatgcgaa cagcagagga
    10801 gtagattcat tcggtatgaa tatcggtgga aaacatgcgt ttccattcgg cgtggtaaaa
    10861 ttcgtacatg cacagcatag ttcaagctat gaagtagacg gtatcgttca gtatatgggc
    10921 gagcctagcg gaattattat tcaagcagaa ggaaaaacga tttatcatgc aggggatact
    10981 gcttatttca gtgatctagg attgcttgca gaagaattcg atattgatgt cgctttcttg
    11041 ccgattggcg ataactatac gatggggcca gaagatgcag caaaggctgc gcaaacaatt
    11101 cgtgccaaga aagtagttcc aatccattac aatactttcc ctgtcattga acaagatccc
    11161 gaatcttttg tagagttact ggaagatagt gaaggaaaaa taatgagcgt ctcggaaacg
    11221 ctggaagtgt aggcagaaaa tggcgacaaa acatgatttg attttagaat atattgaaag
    11281 tttacctgtt ggcaatcgaa tttctgtaag aagcatcgca aaagaattga atgtcagtga
    11341 aggaacagcg taccgtgcaa tcaaagatgc agaaaatgtc ggactagttt caacaattca
    11401 acgagtaggg accatccgta tcgaacgcaa attaaaaaaa catattgaac gtttgacatt
    11461 tggcgaggtc gtcaggatca ttgaagggga cgttttaggc ggggcggcag gattagacaa
    11521 ggtcttgaat aaattcgtca tcggcgcgat gactaaacat gccatggaac gctatattac
    11581 gccaggatca ctaatgatcg tcggcaaccg ggcggaagtc caaaaattag cattggaaga
    11641 tggcgccgca gtattgatca caggaggatt caatacgact gctgagattg caaagctagc
    11701 tgatgatctt gaaatgccga tcttacgcac ctcttatgat acatttacag tcgcaaccat
    11761 gatcaaccga gcgttaagcg accaattgat caaaaaagat atcatgcttg tcagtgatat
    11821 ctatatgcca gctgaaaaga ctcattattt gcagcagaca gatacgattt ctgattatca
    11881 gcgtttgtca gaagaaacgc agcattcgcg ttttccagtc gtcaaccgcc atcatcgttt
    11941 gatggggatt gttacggcaa aagatgtttt agggaaatcg ccaaaccaga tcatcgatcg
    12001 ggtgatgacg aaagaaccaa tcagtgtaaa gaaaacaatg agtattgcct ctgttagcca
    12061 tcaaatgatc tgggatgggc tagaagtcat gccggtcgtt tccgatgact tgacattgga
    12121 aggcttgatt acccggcaag atgtaatgaa agcgatgcag cttgtccagc gacaaccgca
    12181 gattgcagat acgatttcgg atcagatatc tggaaatatc catacgattg atactgaccg
    12241 tgaaggaaat cgtttggaaa accctaagtt caaattcgta gtagcaccac aaatggtcaa
    12301 tggcgttgga accatttctt ttggcgtact tagcgaaatc atttctgatg tcgcacaaaa
    12361 aacaatggtg atgaaccaaa aacgaaatat cttgattgaa caagtgaacc tacattattt
    12421 gcggctgatc caattggaaa gcgaattaga cattcgtcca cgtgtattgg aaatcggcag
    12481 acgttccgct aaactggata tcgaagtgtt tattgaaaac accatcgttt ccaaagcaat
    12541 cgttgtttgt caagtaatgg aacgttctta gaaagcagga gtgaaatgac agtacagcaa
    12601 gaaatagtag atacaataaa aaaatacgat aaaatcatta tccacaggca tttgcgccct
    12661 gatcctgatg cattaggatc acaagtagga ttagccgaac tattacggga aagtttccca
    12721 gaaaaagaaa tacgtcaagt aggtgaaacg attgaagggc ttcgcttttt agcagaaatg
    12781 caagaagttg aagatgtctt ctaccaagga gcgctagtca tcgtgactga tacagctaat
    12841 tcacccagaa tcagtgatca aagatatcag ttggcagaaa aactgatcaa aatcgaccat
    12901 catcccaatg atgaccctta tggagatctt gtgtgggtaa atacaaaagc aagtagctgt
    12961 agtgagatga ttgcagaatt tgctttgatg tttcctgaat ctttaaagat gaacgttaag
    13021 gctgcacggt tactttatgc aggaatcgtc ggagatacag ggcgcttttt gtatccctcc
    13081 acgacgtccc gtacattaga gattgcttcg atattgagaa attatccatt tgatgcaagt
    13141 gctttgaaca gagagattga acaaatgccg atgaaagtcg ctaaactttc cggttatctt
    13201 tatcaaaata tccaggtgga tgaaaatggt gcaggaagaa taatccttcc gcaagaaatt
    13261 cttgataaat atggaatcga tgattcagag acagcttcga tcgtttcact tcctggagtg
    13321 attgacgaag tgctggcttg ggggattttt gttcagcagc cttcagggta ttaccgcgta
    13381 cgtttacgtt ctaaggggcc gatcatcaat gaattggcta aacgccatca tggaggaggc
    13441 catccattag caagtggtgc gaatgcaaaa gatttagcag aagtagagga aatctataaa
    13501 gagatccaag atatatgcca atcgtataag agcagaaaaa cagaatgata cttcgttcga
    13561 aaacgtgcct tttgtagtaa aaatcacaaa tgtatctttt caagatcttc tttttccgtt
    13621 acaatagtgc tcactaaaga ttttaaagga gaaagaagat gacattacca aattgcccag
    13681 aatgtggctc agagtacaca tatgaagatc gaggcttgtt gatttgccca gaatgcggga
    13741 acgaatggaa caaagaagaa caagaaactg tcatagaaga cggattagtg gtattagatg
    13801 caaatggaaa ccgtctgtta gagggcgata gcgtgacagt gattaaagac ttgaaagtaa
    13861 aaggtgcgtc aggtgccatt aaacaaggaa caaaagtcaa gaacatccgt ttagtagaag
    13921 gcgatcataa tatcgattgc aaggtagaag gatttggacc gatgaaactg aaatcggaat
    13981 ttgtgaaaaa gaattaaaga atattcaagg tcttttcaca ttcttttcct actactaatg
    14041 ataaaataaa gtaaacgaaa aggagtgaaa cgaaaatgta tggttatgga atgtatggtt
    14101 ttttagatcc gacatttctt ttggtcattc ttggattagt tatttccgga attgcgtcag
    14161 cgtacgtcaa tagtactttc cgaaaatacg atcaagtgag aagcaaaaac aatgtgactg
    14221 gtacacaagc agcacaatat atattacaaa gtcaacagat aaataatgtc ggtgttcaac
    14281 aaattgccgg agatctcaca gataactaca attcaggtaa taagatgttg agtttgtctc
    14341 aagcaactgc acagtctact tcagttgctg cgataggtgt agcagctcat gaatgcggac
    14401 atgcggtaca agatgcttcg ggttatgtcc cattgaagat tcgtgcagca cttgttccag
    14461 cagctaactt tggttcgatg atttcgttcc ccttgattat ggtaggtgta ttgcttagct
    14521 ggaactctac gttaatcaat atcgggattt tcgctttttc tttagcatta cttttccaat
    14581 tggtgacatt accagtcgaa tttaacgcgt ctcggcgagc aatccaaatt ttgagtgaag
    14641 gcggactact gacggaagaa gaagtaccga tggcaagaca cgtattattt gcagcagcac
    14701 tgacttatgt tgcagcagcg ctttcgacat tcctgcagct gttgcgcttg atcttattgt
    14761 tcggcggcaa ccgaagagat taaaataaag aagttgtgaa atcagcgctt tgatctgagc
    14821 taataccgag agttagctga ttgaatacca tttatttgga ata
//
LOCUS       DAFGZE010000069        14686 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.071, whole genome shotgun sequence.
ACCESSION   DAFGZE010000069 DAFGZE010000000
VERSION     DAFGZE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.071"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="LTU54_002262"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="LTU54_002262"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBL8296958.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /locus_tag="LTU54_002263"
     CDS             952..1257
                     /locus_tag="LTU54_002263"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL8296959.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="LTU54_002264"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="LTU54_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBL8296960.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="LTU54_002265"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="LTU54_002265"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBL8296961.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /locus_tag="LTU54_002266"
     CDS             4417..5355
                     /locus_tag="LTU54_002266"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBL8296962.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="LTU54_002267"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="LTU54_002267"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBL8296963.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="LTU54_002268"
     CDS             6726..7466
                     /locus_tag="LTU54_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBL8296964.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="LTU54_002269"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="LTU54_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBL8296965.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="LTU54_002270"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="LTU54_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBL8296966.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /locus_tag="LTU54_002271"
     CDS             10609..11259
                     /locus_tag="LTU54_002271"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBL8296967.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="LTU54_002272"
     CDS             11260..11898
                     /locus_tag="LTU54_002272"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBL8296968.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="LTU54_002273"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="LTU54_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBL8296969.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="LTU54_002274"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="LTU54_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBL8296970.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..>14686
                     /locus_tag="LTU54_002275"
     CDS             13675..>14686
                     /locus_tag="LTU54_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296971.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STT"
BASE COUNT         4938 a         2729 c         3192 g         3827 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttcaac agaagaatca acagcgcctg aatcttcagc aacagaagaa
    14641 tcaacaacag cgcctgaatc ttcagcaaca gaagaatcaa caacag
//
LOCUS       DAFGZE010000070        14625 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.072, whole genome shotgun sequence.
ACCESSION   DAFGZE010000070 DAFGZE010000000
VERSION     DAFGZE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.072"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            4..816
                     /locus_tag="LTU54_002276"
     CDS             4..816
                     /locus_tag="LTU54_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBL8296972.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            813..1613
                     /locus_tag="LTU54_002277"
     CDS             813..1613
                     /locus_tag="LTU54_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBL8296973.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1774..3042
                     /gene="rseP"
                     /locus_tag="LTU54_002278"
     CDS             1774..3042
                     /gene="rseP"
                     /locus_tag="LTU54_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBL8296974.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3110..4819
                     /locus_tag="LTU54_002279"
     CDS             3110..4819
                     /locus_tag="LTU54_002279"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBL8296975.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5031..9389
                     /locus_tag="LTU54_002280"
     CDS             5031..9389
                     /locus_tag="LTU54_002280"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBL8296976.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            9534..10007
                     /gene="rimP"
                     /locus_tag="LTU54_002281"
     CDS             9534..10007
                     /gene="rimP"
                     /locus_tag="LTU54_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBL8296977.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10030..11205
                     /gene="nusA"
                     /locus_tag="LTU54_002282"
     CDS             10030..11205
                     /gene="nusA"
                     /locus_tag="LTU54_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBL8296978.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11227..11520
                     /locus_tag="LTU54_002283"
     CDS             11227..11520
                     /locus_tag="LTU54_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBL8296979.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11517..11828
                     /locus_tag="LTU54_002284"
     CDS             11517..11828
                     /locus_tag="LTU54_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HBL8296980.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11841..14147
                     /gene="infB"
                     /locus_tag="LTU54_002285"
     CDS             11841..14147
                     /gene="infB"
                     /locus_tag="LTU54_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBL8296981.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14174..14521
                     /gene="rbfA"
                     /locus_tag="LTU54_002286"
     CDS             14174..14521
                     /gene="rbfA"
                     /locus_tag="LTU54_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBL8296982.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4983 a         2598 c         3255 g         3789 t
ORIGIN      
        1 agaatgttac gtttttttcc gcaaaaaaac aaatatattc aagaaaaaag cgaatttcaa
       61 tttaataccg atggaccaat tcctcagcac attgccatca tcatggacgg caatggtcgt
      121 tgggcgcaaa accgtcgttt gccacgagtt gctggccata aagaaggcat ggaaacagta
      181 aagaaagtca caaaaaaagc atcaaggctt ggagtgaaag tgttgacgct ttatgccttt
      241 tcgactgaaa actggaagcg accaaaagat gaagtaagtt ttctcatgca actacctgtc
      301 gacttctttg atacgttcgt accagaattg attaaagaaa atgtcaaagt tcatgtcatg
      361 ggttatgaaa acgtattgcc agaacatacg caagatgctg taagacgtgc aattgagcaa
      421 acaaaaaaca atactggaat ggtattgaat tttgctttga attatggaag tagagcagag
      481 atcgtgactg ctgtccaaga aattgccgaa gaagtagcaa aaggagaaat acacgcagaa
      541 gaaatcgatg atgaattgat tgctaagcat ttaatgacag gtttcttgcc taaagagtta
      601 caagatcctg agctgatgat ccgtacaagt ggagaagagc gaattagtaa ttttctgtta
      661 tggcagatcg catatagtga gctgtatttt acaaaagcgc tttggcctga ttttgatggt
      721 gcgcatcttg aagaagcaat cgcctcttat caaaatcgag accgtcgatt cggcggtgta
      781 aaaaaaacag aagatgagaa aggggatcag tcatgaaaca acgtgtgatc acagcagctg
      841 tggcgctagt cttgtttatt ccgattatat ggatcggagg catagcggtt gagcttacag
      901 cagcaatctt agcggtagta ggagtttacg agctgttccg gatgaaagga ttgacactct
      961 taagttttga aggagtttta tccgccgttg gtgcagtgtt acttgttttg ccaaaagaaa
     1021 gatggttttt ctttcttcca gagaatgtaa gtacatttat gttattttat cttatagtta
     1081 tgattctttt aggaacatca gttatttcta aaaatacgta tacgattgat gaagcaggtt
     1141 ttcctgtgct tgtcagcttg tatgtgggaa taggtttcca gaattttgtc aatgcacgaa
     1201 cagaaagctt ggctgttttg atatttggtc tatttattgt ttgggcaact gatatcggag
     1261 catatatggt tggtcgacgt tatggtcagc ataagttaag tcctgatatt tcgcccaaca
     1321 aaacgattga aggtgcgctc ggtggtatcg gtagtgctgt tgtagtcgca tttctttatt
     1381 tattgctata cccagcaaaa gaactattca atcatggtat gtttgtcatg cttctgatga
     1441 cgattatttt ttcagcagtc ggacaatttg gtgatttggt cgaatctgca atcaaacgtc
     1501 attatgatgt gaaagactcg ggaaatatct taccaggcca tggtggaatc ttagaccgtt
     1561 tcgacagttt gctgtttgta tttcctatca tgcatttatt tggcattatt tagtaagtat
     1621 acctgcgttt ctttgtgaat ttcataaaag acgcaggttt ttatttttca ttatattgat
     1681 actattggtg tctttggtat actatggtaa aattatagtg ttttatcgtt gttttataaa
     1741 aaacacaaaa aacaagtaaa ggagaaaaaa gctatgaaaa cgattctgac atttatcatc
     1801 gtttttggta tattagtgat tgttcatgag tttggtcatt tcttctttgc gaaacgatca
     1861 ggaatcctcg tccgcgaatt tgctatcggt atgggtccga aaatttatgg gcatcaagca
     1921 aaagacggga caacatatac gcttcgccta ttacctattg gcggttatgt tcgaatggca
     1981 ggcaatgggg atgatgaaac agaaatggca ccaggaatgc ctctttctct tttactaaat
     2041 tctgacggca tcgtagagaa gataaattta agtaagaaaa tccaattgac aaatgctatc
     2101 ccgatggaat tgagccgtta tgatttagaa gatgagctga ctatcactgg ttatgtgaat
     2161 ggagacgaaa cagaagtagt aacctaccct gttgatcacg atgcgacgat tattgaaaac
     2221 gacggaacag aaatcagaat tgcgccaaaa gacgtacagt tccaatcggc taaattgtgg
     2281 cagcgtatgc tgaccaactt tgctggaccc atgaataact ttattttggc aatcgtgtta
     2341 ttcatcattc tggcatttat gcaaggtggt gttcaagtga cgaacactaa tcgcgtagga
     2401 gagatcatgc ctaatggagc tgcggcagaa gcaggcttaa aagaaaatga tgaggtagtc
     2461 agtgtggacg gaaaagaaat ccattcatgg aatgatctga caacagtcat tacgaaaaat
     2521 ccaggtaaaa cattagactt taagatcgaa cgagaaggtc aggtgcaatc tgtcgacgtg
     2581 acaccgaaat ctgtagagtc aaatggcgaa aaagttggcc aacttggtat aaaagcccca
     2641 atgaatacag gttttatgga taagatcatt ggagggacgc gtcaagcttt cagcggttca
     2701 ttggaaattt tcaaagcctt aggatcacta tttacaggtt tcagtttaga taaattaggc
     2761 ggacctgtca tgatgtacca attgtcttca gaagcagcaa atcaaggtgt aacaactgtt
     2821 attagtttga tggcgcttct ttcaatgaat ctcggaatcg tcaatctgct tccgattcct
     2881 gccttagatg gcgggaaatt agtcttgaat atttttgaag ggattcgcgg gaaaccatta
     2941 agtcaagaaa aagaaggaat attgacatta gctggctttg gatttttaat gttgttgatg
     3001 gtgctagtta catggaacga tattcaacga ttctttttct agaaaaagtt gacaaatagg
     3061 gtataataac aaacgataac tattttgagg aaataaagga gtatatagaa tgagacaatc
     3121 aaaaatgttg attccaacat tacgcgaagt tccaaatgat gcagaagtat taagccatca
     3181 aatcttgcta agagctggat atatccgcca agtatcagcg ggtatttatt catatctgcc
     3241 attagcaaat cgtgtattgg aaaaactgaa acgcattatg cgggaagaat ttgaaaaaat
     3301 cggagcggta gaaatgctga tgcctgccat cttgccagca gaactttggg aagaatccgg
     3361 gcgttacgaa acatatggac caaacttgta tcgtttcaaa gaccgcaatg atcgaaaaat
     3421 gatccttgga ccaactcacg aagaaacttt tactgatttg atgcgtaatg aaatcaattc
     3481 atataagaaa ttacctttaa atctttatca aatccaacct aaatatcgtg acgaaaaacg
     3541 acctcgcttc ggtttgcttc gcggaagaga gtttatcatg aaagatgcat attctttcca
     3601 tgcaacggaa gaaagtttgg acgagacata taaagattac gaaaaagcct ataccgaagt
     3661 attcaagcgt tgtggcttag aatttcgctc gatcatcgga gacggcggcg caatgggcgg
     3721 gaaagactca aaggaattca tggcgatctc agaaatcggg gaggatacga tctgttattc
     3781 gactgaaagt gattatgctg caaacttgga gatggctaca agctactatg tatcaaaaaa
     3841 atcgcatgaa acacagcttg aattggagaa agtagctaca cctaacgtaa aatcaattga
     3901 cgaagtagca gatttctttt ctgttgaacc tcaaaaaatc atcaagtctg tattgttcat
     3961 tgcggatgaa caacctgtcc ttgttttggt acgtggagac catgaagtca atgacgtaaa
     4021 actgaaaaac tttttatctg ctgacttttt agaagaagca acagaagaag aagcacaaaa
     4081 atatctaggt gctgagtttg gttctgtcgg accagtcggt gtttcagatg acgtaaaagt
     4141 atatgcagac cgttatgtcc aagatttagc gaatgccatt actggagcga acgaaacagg
     4201 attccactat aaaaatgtga atccagaacg cgatttcact gtgctttctt atgaagatct
     4261 tcgttttgta caagaaggag atccttcacc agacaacaac ggtgttcttg cctttactcg
     4321 aggaatcgaa atcggacata tattcaaaat cggcactcgt tacagtgaat ctatgggagc
     4381 aactgtacta gacgaaaatg gacgagaaaa atttgtgatt atgggctgtt atggtatcgg
     4441 tgtaagtcgt ttattatcag caatcgtaga acaacattca gatgaacaag gaatcaattg
     4501 gccaaaagga attgcgccat ttgatctgca tatcgtccaa atgaatctaa aagacgaata
     4561 ccaaacaagc ctgacgaatg aactagaaga agcgatgaca aaagctggct atgaagtact
     4621 ggttgatgac agaaatgaac gcgcaggagt gaaattcgcg gattctgatt tgattggctg
     4681 tccaatcaga atcactgtcg gtaaaaaagc agtggatgga atcgtagaag tgaaaatcaa
     4741 gaaaactggc gaaatggttg aagtacgtaa agaagaattg gcgaatactt taccaatcct
     4801 cttgaaccaa ggcgaataat aagcgttaaa acacagaaaa taaaaatagc tgaatcagct
     4861 gtttttgaca atctgatgat tcgcttttaa aattggaact gcgatttgaa tcgcagttcc
     4921 aattttttaa tgtaaaactc agaaaaaacg agtatactaa aaggtactga aaaagaagag
     4981 gataagatgc gatcaattgc attgtttctc atcagaaagg gcgtatatct ttgtcagaaa
     5041 aacgagattt atttgaaaaa ctgttggaac aaatccaact agaggaagca gaaaaaaatc
     5101 atccactgct tactgctggt gaaatggata gtgtagtggt ccatcgtaaa tcgcgtttat
     5161 gggaatttac gctacatttt tccaaaattt tgcctattat gctatatcgg tcattgactc
     5221 agcatctgac tatcgctttt aaagatattg cacaagtcaa actgaatatc ttagcagatg
     5281 accagacatt cgatgaacag ctcttgcaag attactgggc ccaagctttg gaaaatcagc
     5341 aatgcgatac accactagca cagcaagtat tgaaaacgca ggtacctgtg ataaaagatc
     5401 gtaaagtcat cttgccaatc gacagtacag gagctatttc ttatttgaag caacaatact
     5461 tgccattagt agaagagttg ttcgtcagtt acggattccc aaaatttcgt atcgaaccag
     5521 aagtggatga gcagcaagcg gaacgagtat tgaaactttt tgaagaacgc aaacaagaac
     5581 aggcagaagc ctttatgaag caagcagctg aaagtctagt tgtccacgaa caaaagaaaa
     5641 aggaaagaaa agagcaatcg ccagctcttg aaggacccat ccacatccag ctaggacgaa
     5701 atattccagc cgatgaacca acgacaccaa tgatcagtat cgttgaagaa gaacgaagag
     5761 tcacacttga ggggtacgta ttcgacaaag aagtcagaga actacgttca aaacgcaaga
     5821 tcttgacttt gaaaatcaca gattatactt catcatttat cgtcaaaaaa ttctcgaacg
     5881 gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg tagctggtta aaggtacgag
     5941 gaagtattca agaagatacg tttgtccgtg atttggtcat gaatgcgcaa gatatcatag
     6001 aagtcaaaca tacacctcgc aaagattatg ctcctgaagg ggaaaaacga gtagagcttc
     6061 atgttcatag caatatgagt acgatggatg caacaaacag catctctgat cttgtggcac
     6121 aagcagggaa atggggacac cgagctatcg ccattacaga ccacggtggt gcgcaagcct
     6181 ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa aatcttatac ggcgtggaag
     6241 cgaatgtcgt agatgatggt gtcccaattg cttataatga tgcacatgaa gcattgagtg
     6301 aagccacata tgtcgtattt gacgtggaaa cgacaggatt gtctgcagta tatgacacga
     6361 tcatcgagct agccgcagtc aaaatgtata aaggaaatgt gatcgaaagc tttgatgaat
     6421 ttatcgatcc aggacatcct ttgtcacgaa caacggtaga tctgactggt atcacggatg
     6481 gaatggttcg aggatcaaag tcggaagaag aagtattgcg gatgttcctt gaattttcaa
     6541 aagatacaat tttggtagcc cacaacgcgg cttttgatat ggggttcttg aatactagct
     6601 acgcaaggta tggtattcct gaagcggcca atccagtcat tgatacgttg gaattagcta
     6661 gatatctgta tcctcaattc aaacgtttcg gattaggtgt gttatctaaa aaatttggtg
     6721 tcagcttgga acagcatcac cgagcaatct atgatgctga agcaactggt cacttagcat
     6781 ggatctttgt gaaagaggca atggacaatc ataacatgct ttatcatgat cagctaaatg
     6841 agcatatcgg agaaggagat tcttacaaaa gggctcgacc tttccatgta acgattttag
     6901 caaaaaatca ggcaggactg aaagatttgt tcaaactgat ttcaatgtcg aatgtcgaat
     6961 acttcgaacg cgtcccacga ataccacgct cccaattgaa aaagatgcgt gaaaatctat
     7021 tgatcggctc agcttgtgat aagggagaaa tattcgaagc catgatgcaa aaaggggtag
     7081 aagaggcaag aaaccgagca aaattctacg attatattga agttatgcca aaagcggtct
     7141 atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca tgatttggaa gaaattatcc
     7201 aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt tgttgcaaca ggtaatgtac
     7261 attatctgaa cgaagaagat gccatttacc gaaaaatttt gatcaattct atgggcggag
     7321 cgaatccgtt gaatcgtcat agtttgccgg atgtccattt ccgaacaacc gatgagatgc
     7381 taactgcatt tcacttttta ggggaagaaa cagcgaaaga gatcgtagta gaaaatacga
     7441 ataaaatcgc agatatctgc gaagaagtga ttccagtcaa agatgaactt tatacaccga
     7501 agattccagg ttcagaagat gaaatatcag aattgagtta cacaaaagca aaacaaatgt
     7561 acggagatcc gttacctgag atcattcaaa aacgattaaa aaaagagttg aattctatta
     7621 atggtaatgg cttctcggtt atttatctga tcgcccaaaa actagtgcat aaaagtaatg
     7681 aagatggcta tctagtaggt tcacgtggct ctgttggctc tagctttgtt gcgacgatga
     7741 caggaatcac tgaagttaat ccgttagccc ctcattatta ttgtccagag tgccaatatt
     7801 ccgagttttt tgaagatggt acgtatggtt ctggatttga catgccagaa aaacaatgtc
     7861 caaaatgtgg tgcacgctta aacaaagacg gtcatgatat tccatttgag acatttttag
     7921 gattccatgg agacaaagtg cccgatattg atttgaactt ctcaggtgat tatcaagcag
     7981 aagcccataa ctatacgaaa gtattgtttg gcgaagacta tgtttaccgt gccggaacga
     8041 ttggtacagt cgccgacaaa acagcatatg gctatgtaaa aggatacgaa agggacaata
     8101 atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa aggcgctact ggagtcaaac
     8161 ggacaaccgg ccagcatcca ggggggatca tcgttattcc cgattatatg gatgtctatg
     8221 attttacgcc gatccaatat ccagcagacg atcaaaattc ggaatggaaa acgactcatt
     8281 ttgattttca ctcgattcac gataatgtat tgaaattaga tattctaggg catgatgacc
     8341 caactgttat tcggatgctt caagatttat cagggatcga tccacaaacg atccctacgg
     8401 atgacccgga agtgatgcgg atatttgccg gtcctgaagt acttggagta agtcaggaac
     8461 aaatttattc caaaacaggg acattaggta ttccagaatt cggcactcgt tttgttcgag
     8521 ggatgctaga agaaacgcat cctacaactt ttgcagaact actgcaaatt tccggactat
     8581 ctcatggtac agacgtatgg ttaggaaatg cggaagaatt gattcgacga ggagatgcga
     8641 cactggcgga agtaatcggc tgtcgtgatg atattatggt ttatttgatc catgctggat
     8701 tagatagcgg gatggcattt aaaatcatgg aaactgttcg taaaggacaa tggaacaaga
     8761 tccctgatga attgcgagaa acctatctat cggcgatgaa agaaaataat gtgcccgact
     8821 ggtatatcga ttcttgttca aaaatcaaat atatgtttcc gaaagcccat gctgcagctt
     8881 acgtattgat ggctcttcgt gtcgcttatt tcaaggttta tttccctatt ttgtattact
     8941 gtgcatactt ttctgtacgt gcagacgact ttgatctagt ctccatgtgc aaaggaaaag
     9001 atgctgtgaa acaagcaatg aaagaaatca cggacaaagg attagatgct tccgtaaaag
     9061 aaaagaatca actgacagtg ctggagttag ccaatgaaat gctggaacga ggattcaaat
     9121 ttggaatgat tgatttgtat aaatcggatg ccgttaactt tgttattgaa ggtgatacgt
     9181 tgatcgcacc atttagagct gtcccaagtt tgggaaccaa cgtggcaaaa caaatcgttg
     9241 aagcaagaaa agacggtcca tttttatcca aagaagatct tgccacccgc ggtaaagttt
     9301 caaaaacatt gatcgaatat atgaacgaca atggcgtatt gaaagatcta ccagatgaaa
     9361 atcaattatc gttattcgat atgctataag catctgtttt cctgtctgct tgtaatttta
     9421 agcagagtat ggtatagttc ttataggtaa tgatactaac tgtgagtgag cggatttttc
     9481 gctcactctt tttaatggaa ttacgcactt aattttttaa ggaggcgaaa cttttgagta
     9541 gcgtcgttga aacagtcaca gaaatggtga caccgatttt agatgaacaa aattttgaac
     9601 ttgtcgaagt cgaatttgta aaagaaggaa aaagctggtt cttacgagta tttattgaca
     9661 aagaaggcgg tatcgatatc gaagaatgcg cctttgtcag tgagaaactg agtgaaaaac
     9721 ttgatgcagc agatccagat ccgattcctc aagcttattt cctggaggta tcctccccgg
     9781 gagcagagcg gccattgaaa aaagaaagcg actatgaaaa ggcacttggt gattatgtcc
     9841 atgtctcctt ataccaacca gtagatggcg aaaaacaata tgaaggattt cttcaatcat
     9901 ttgatgctga acagcttaca ttgaaaatac gcataaaaac acgagaaaaa gaattgactt
     9961 ttgaccgaaa aaatattgca aaagctcgct tagctatcca attttaatca cggagaggaa
    10021 tcagaaaaaa tgagtaaaga aatgttaaat gcgttagacg ctttagaagc tgaaaaaggg
    10081 atctcaaaag aaatcgtgat cgatgcttta gaagcagcat tggtttctgc atacaaacgt
    10141 cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc agaaaaaagg aaagatccat
    10201 gtctatgcgg taaaagaagt aacggaagaa gtgatggatt cacagttgga agtctcattg
    10261 aaagatgcac ttttaatcaa tccagcatat gagatcggtg atacgatccg ttttgaagta
    10321 acaccaaaag attttgggcg gatcgctgcg caaacggcta aacaagttat tttacagcgt
    10381 gtacgtgaag cagaaagaac gattatttat aacgaattca gtgcttatga aaaagacatc
    10441 atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt atgtcaatct tggtaaaatt
    10501 gaggcagttc tttctaaaca agatcaaatg ccaaatgaat tttatcaacc acatgatcga
    10561 atcaaagttt atgtttcccg agtagaaaat acgtctaaag gtccgcaagt atttgttagc
    10621 cgcagccatc cagacctatt gcgccgctta tttgagcaag aagtaccaga agtctatgat
    10681 ggaatcgttg aaattgtcag catcgcccgt gaagctggcg atcgttcaaa agtggcagtt
    10741 cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg tcgggccaaa gggacaacga
    10801 gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg atatcgtcga atggaatgaa
    10861 gatccagctg tattcattgc aaatgcattg aatcctgcac aagtcatgga tgtgatcttc
    10921 gatgagacaa atccaaaggc ctgcacagtt gtcgttccag actaccaatt atcgctagca
    10981 atcggtaaac gcggacaaaa cgcacgttta gctgctaaat tgactaacca taaaatcgat
    11041 atcaaatcag aatctgacat gacggaattt tatgaaaatc aagaacaaca aaaagaaaca
    11101 gaagaactgc atgatgaagc aatcgttcag tcagatttaa cagacgatga atatgaaaca
    11161 attgcattca acaatgaaac ggtcgaagaa aaaccagaag cttaacttta gtaaggaggc
    11221 aaagagatga aacaaaggaa aattccttta cgcaaatccg tggtttcagg tgaaatgaaa
    11281 cctaaaaaag agatggtccg aatcacccgt tcaaaagaag gggaagtatc gatcgatcca
    11341 actggaaaaa tgcctggacg aggagcgtat gtctcacttg agccggaaga agtacaacaa
    11401 gcttgggata aaaaaattct cgatcgagta ctcgaaacaa cattaacaga tgagttttat
    11461 caagaacttc ttgactatgt cactcaccaa aaagcacgta aagagttatt tggaaaatga
    11521 atacagttaa tcggcaaaaa gccatgaatc tcatcggtct agcgatgcgc gcgggaaaac
    11581 taatcacggg tgaagaactg accattgctg aaatccgcaa acaaaaagca aaattggtgt
    11641 ttgtggcaac cgatgcaagc gagaatacaa gaaaaaagat aaaagataag agttcatatt
    11701 atgaagttcc ttgctttgag ctattttctg aagcagaaat cacgcagatg atcggaaaac
    11761 ctcgcaaggt attcggaata atggatgccg gatttgcgaa gaagaccaag gagctaattg
    11821 aaggttagga aggtgattgc atgggcaaca aaagaatcta tgaattagca aaagaactca
    11881 aaaaatctag taaagacgtc gtggaaaaag cacaacaact tggcatcgat gtgaaaaacc
    11941 atatgggtgc gattactagc aatgatgaga agaaattgca acaagcgttc aaaaacccaa
    12001 gcacgaaaca acaagctgca caacaagcaa gcagaaaacc agccaatcaa cagccgaacg
    12061 atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa taaaaataac cgcagttatc
    12121 aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa aaaaccaaca acaaaccaaa
    12181 ataatacggg aaataccagc agccaaaatc gtcaaggaac gagccaaggc ggcaatcaaa
    12241 accgtactaa caataacaac aaaccaaaca ataacaatcg cggcaaattt aataataata
    12301 accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca gcaacaatca actaaaccag
    12361 ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt agagtatact gaaggtatga
    12421 acgtagcaga tattgcgaaa aaaattcatc gtgagccagc agaaatcatt aaaaaattat
    12481 ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga taaagatacg attgaactgt
    12541 tagcgacgga ctatggcatg gaaccacaag aaaaagtcca agttgacatt gctgatatcg
    12601 ataaattctt cgaggcagaa gaagtcgatc cagataaatt agtctcacgt ccgccagtcg
    12661 taacgattat gggacacgta gaccatggga aaacaacttt attggataca ttacgccatt
    12721 cacgtgtaac aagcggcgaa gccggcggaa tcacacagca tatcggtgcc taccagatcg
    12781 atattgatgg taaaccaatc actttcttgg atacaccagg acatgcggcc tttacaagta
    12841 tgcgtgcacg tggagcaagt atcacagata tcacgatctt agttgttgct gcagatgatg
    12901 gcgtcatgcc acaaacagtt gaagcaatca accatgccaa agctgcaggc gttccaatca
    12961 tcgtcgctgt aaataaaatt gataaaccag gtgcaaaccc tcaacatgtt atgcaagaat
    13021 taagtgagta tgagttaatt cctgaagcat ggggtggaga aacgatcttt gttgagatct
    13081 cagcgaaatt cggccaaaac atcgaagaat tgctggaaat gatcctgtta gtcgcagaag
    13141 tagaagattt aaaagcagat ccaacacaac gcgcaatcgg aacagtgatc gaagcacgtc
    13201 ttgataaagg taaaggtcct gtgtcaacct tacttgtcca agaaggaaca cttcatgttg
    13261 gtgacccaat cgttgtcggt aatacttacg gacgtgtccg tgtaatgaca aacgatcttg
    13321 gccgtcgcga taaagaagca ggacctgcaa cgccagtaga aatcactggc ttgaatgatg
    13381 tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga aaagacagca cgtgcagcgg
    13441 gtgaagaacg cgcgaaacgt gcattattag aaaaccgtgc tgccacaagc cgtgtgactt
    13501 tggataactt atttgaaagc ctgaaagaag gcgaattgaa agaagttaac gtaatcatca
    13561 aagcagatgt acaaggttca gcagaagcac tggctgcatc attgaagaag attgatgtgg
    13621 aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc aatcaatgaa agtgatgtaa
    13681 ccttagcagc agccagcaac gctattatca tcggtttcaa cgttcgtcct acaccgcagg
    13741 caaaagtgca agctgatact gaagaagtcg atattcgtct tcaccgtatc atctataaag
    13801 ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga ccctgaattt gaagaaaaaa
    13861 tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc taaagttggt acgattgcag
    13921 gtgcctatgt aacagaagga tatattcgta gagatagcgg tgtccgtgta atccgtgacg
    13981 gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg attcaaagat gacgtgaaag
    14041 aagtcaaaat gggttatgaa tgtggtgcaa tgattgaaaa attcaatgac atcaaagtag
    14101 atgacgtaat tgaaggattt atcatggaag aagtcaaaaa tgactaaaaa ctgataaaag
    14161 gaggccaatc attatggcta actaccgtga ccgccgagtc ggtcaagaaa tcttaaaaga
    14221 agtcaatgat attttgcgca aaaaggtccg tgaccctcgt gtggagaacg taacgatcac
    14281 agatgtgaaa gttacggggg acttacagca agcaacgatc tattacagca ttttatctga
    14341 cttagcatca gataagaaaa cagcacaaga tggattgaat aaagcatcag gattgattcg
    14401 tcgagagtta gggcacaatc taagtattta taagacacct gaattgttct ttgaacttga
    14461 tgaatcagtt gtctatggca accatatcga tgaattgctt cgcgacttga acaaagacta
    14521 atcaaaaact cccgcaaatc ctgtgaaatc aggctctttg tcaataagga ctgatgagtt
    14581 gtgcaaaatc aaatctgagt cagaatgaac cacattctgg ctcag
//
LOCUS       DAFGZE010000071        14529 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.073, whole genome shotgun sequence.
ACCESSION   DAFGZE010000071 DAFGZE010000000
VERSION     DAFGZE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.073"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(334..696)
                     /locus_tag="LTU54_002287"
     CDS             complement(334..696)
                     /locus_tag="LTU54_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296983.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(770..1150)
                     /locus_tag="LTU54_002288"
     CDS             complement(770..1150)
                     /locus_tag="LTU54_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBL8296984.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1214..2407
                     /locus_tag="LTU54_002289"
     CDS             1214..2407
                     /locus_tag="LTU54_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL8296985.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2483..3067)
                     /locus_tag="LTU54_002290"
     CDS             complement(2483..3067)
                     /locus_tag="LTU54_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8296986.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3268..4266
                     /locus_tag="LTU54_002291"
     CDS             3268..4266
                     /locus_tag="LTU54_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBL8296987.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4378..4887
                     /locus_tag="LTU54_002292"
     CDS             4378..4887
                     /locus_tag="LTU54_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBL8296988.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4917..5576)
                     /locus_tag="LTU54_002293"
     CDS             complement(4917..5576)
                     /locus_tag="LTU54_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBL8296989.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5578..6342)
                     /locus_tag="LTU54_002294"
     CDS             complement(5578..6342)
                     /locus_tag="LTU54_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HBL8296990.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6350..7027)
                     /locus_tag="LTU54_002295"
     CDS             complement(6350..7027)
                     /locus_tag="LTU54_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HBL8296991.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7064..8449)
                     /locus_tag="LTU54_002296"
     CDS             complement(7064..8449)
                     /locus_tag="LTU54_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL8296992.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFYLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8659..8743)
                     /gene="ffs"
                     /locus_tag="LTU54_002297"
     ncRNA           complement(8659..8743)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="LTU54_002297"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8766..9281)
                     /gene="tadA"
                     /locus_tag="LTU54_002298"
     CDS             complement(8766..9281)
                     /gene="tadA"
                     /locus_tag="LTU54_002298"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBL8296993.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9388..10245
                     /locus_tag="LTU54_002299"
     CDS             9388..10245
                     /locus_tag="LTU54_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296994.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDGTEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10326..10661)
                     /locus_tag="LTU54_002300"
     CDS             complement(10326..10661)
                     /locus_tag="LTU54_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8296995.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10728..12098)
                     /locus_tag="LTU54_002301"
     CDS             complement(10728..12098)
                     /locus_tag="LTU54_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBL8296996.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12176..12712)
                     /locus_tag="LTU54_002302"
     CDS             complement(12176..12712)
                     /locus_tag="LTU54_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8296997.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13021..14502
                     /locus_tag="LTU54_002303"
     CDS             13021..14502
                     /locus_tag="LTU54_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8296998.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4477 a         2891 c         2359 g         4802 t
ORIGIN      
        1 aacgactcag ttatcttatc atgtcgttag taacatgtca ataactattt tttaaatttt
       61 ttagaaatgt gatataacat ccacttcaga atcaacgctg ttaaatttat catgtatttc
      121 ttccttggtc aactataaat taagcattac tttgtgaaat gatttttcga tcaattatta
      181 ttattcgtta tacaaccgtt ttcaacatat tttgaaaata aaatgcacta aaaaactatc
      241 tttagcagaa catttcattg tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa
      301 aattcaaact tctttgttcg cttaatctgt attttagaag aaaggcactc cctctacttc
      361 gtaagttcca agaacagagc gcatttgacc tggttcttcc caaatatatc cttcgcctaa
      421 ttcttctctt tgattctttt gttcagtttc aaagacaaac aacggaacat tttcctcttt
      481 aatatgtgta tttgtcaatt ccatcaaact aatcttgcta acatcgatat gaactatttc
      541 ttttaagaaa ttaagtatat tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc
      601 aaattccaac ttgtcatcaa ttgaatggac caaaaatttt ttagtaccat cctccaaatg
      661 caacataatt gtaccagcaa ctcttttgct tgccaaaatt tccacctcga tcaacttgtt
      721 ctatggctta cataaaaatt ctagcacatt ttcagaaaaa atacactttt tatttcttat
      781 tttctttccg ttctagttca gataatgctt cttctatcca aataggtaac tgctccgcaa
      841 tagtggtaaa tccgatctta cgatttttat tcgtgaaata cctttttctg cgatatggaa
      901 cagctggatt tgttttttct aacgttctta aagataaact gatttttttg gaatattcat
      961 caatgtcgat gatctgaact gttactggct gtccgacttt taacaatgaa tggatatttt
     1021 tcgtataacc ggattgtact tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag
     1081 aaacgaatgc gccataaggc tgtatccctg tcacttttcc ttcaattatt tgtccaattt
     1141 tataagtcat ctacttccct ctcttcgcat ttatcattat actgtaaata gctcaaaaaa
     1201 agaagcgacg aaaatgactg gaggtcttaa gatgatcgaa tttgataaac agatttcaag
     1261 aaaagataca gaaagtgtaa aatgggacgc aattgctgag acatatcaaa tggatgactt
     1321 acttcctttg tgggtagcag atatggactt cttgtctccc ggtggggtag ctaaagcgtt
     1381 tagtgaatac atcaaacatg gaatctttgg ttatgccact ctgtctgata aactatatca
     1441 ggctatcatt cattgggaac gccaacacca tgctgtggag ttgacgaaag aaaatatcgt
     1501 ttttacaagt ggtgtgttaa ccagtttagc tgcagcagta caaactttta caaaaccagg
     1561 cgattctatt ttgattcatg acccagttta tcctccattt tcctctatta tagaaacaaa
     1621 tcaacgtcac atcgttcgaa gctctttgct agaaaaaaat aatcatttca tcatggactt
     1681 gtctgacatg gagaaaaaaa tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc
     1741 tcacaatcct ggagggcgtg tttggagcaa agaagaattg aaacagttaa gcgaattgtg
     1801 tttaaaacat caagtgattc tgtttagtga cgagatccat caagatttaa ctttattcga
     1861 tcatacattt acttccatgc taacgattga tcctaattta gatgatctac tagttgcttt
     1921 tacttccgca accaagactt tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa
     1981 aaatccagag atgaaagctg attttgaaaa acatttagtg atgaaccaac agcacgaaat
     2041 caacactttt ggactgattg gaacccaagc ggcttatgag acgggtggag aatggttaag
     2101 ccaactgatt ccttatctag aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta
     2161 tcttccgaaa gtgagaataa tgaaccctga aggaacttac ttaatgtggt tagattttac
     2221 cgcctatatt tcagacgacc aagaattgga gaacactctt gttcggaaag gaaaagtcgt
     2281 attaaatcct ggcatcacgt ttggtccttc cggtcatggg catatgcgct tgaatattgc
     2341 ttgtccagaa gaaactttac tggaaggact taatcgaatc aagtatgctt tagagacaat
     2401 cgattaaata tttattcaaa ataaaaggac gagaccttac taggtttcgt cctttttcat
     2461 aattactccg taccatacca aactattctg aaatttcaat tgtttcaatc actacatcat
     2521 gtacaggtcg gtcttgtgga ccacgttgta catttgcgat ttcatctact gtgtccatcc
     2581 cttctagtac atgtccaaag actgtatgtc ggaaatctag ccaaggagtt ccgccacctt
     2641 tatacgcttc aatgatttct gatgggaatc cagcaccttc tagttgtcca agcatattag
     2701 ctggcacatt tgtgttattt acaataaaga attggcttcc gtttgtgttt ggacctgaat
     2761 ttgccataga taaagctccg cgaaggttga acagttcacg actgaattca tcttcaaaac
     2821 tttcgccata aatactttcg ccacccattc ctgttccagt tggatcgccg ccttggatca
     2881 tgaaatcagg aataacgcgg tgaaaaatca cgccatcata ataacctttt ttggaaagtt
     2941 caatgaaatt ttgaaccgtc ttcggtgcta gttcagggaa taattgaaca gtaatgtcac
     3001 cacggtttgt tttgataaca gcttttggtc ctttttcact ttttaaatct agttgtggga
     3061 atgccatatt gtttcctcct aatatttgca tacttcataa taataataac ataataccta
     3121 acctctcgta aaaatactag atttgttttc ttttattttt catcactaga tacattctat
     3181 aacttaccgt ttcgatttcg aattcaaaca tttttgagaa gcattctcaa ttaaattctg
     3241 ctatagtgtg aaaagggagg aatttttatg gaaatctatg acattacaat catcggtgca
     3301 ggtcctgtag ggatgtttgc cgctttttac gcaggaatgc gccaagctaa aacaaaaatc
     3361 attgacagtc tacctcaact tggtggtcaa ttagcaactt tgtatcccga aaaatatatt
     3421 tacgatattc ctgggtatcc agcaataaaa gcaagtgaat taatcgatca attagaaaaa
     3481 caattaacta cttttaacca tacattccat ttaaaagaag aagttctctc gcttactcgt
     3541 gaagacgaag ttatcgagat tactacaaat aaaggcatcc attattccaa agcagtcatt
     3601 ttagctttag gaaatggttc attccagcca agaaaattga atttagataa tgctgaatca
     3661 tttgaaaacc acggattgga ttatttcgtg aatgatttga tgaagtatgc tggaaaaaaa
     3721 gtcgctattg ctggaggtgg tgattctgct atcgattgga cgttgatgtt agagcctatt
     3781 gcaagcgaag tttacttgat tcatcgtcgc ccggaatttc gtgcgcacga acatagtgta
     3841 tcacgcttga aatcttcatc tgtcaacttg cttactccct atctcatcga tggattatca
     3901 ggcaataatg gggaactgac agatatccgg ttaaaaaaag tgaaaagtga cgagacgatt
     3961 gatttgatga ttgattctct gatcgtcaat tatggattct cttctaattt agaacatctt
     4021 tcttcttggg gacttgatag taccagaaat gccattactg taaaatcaga tatgtctact
     4081 tctattccag gcgtttatgc agctggtgac atctgtacct atgaaggaaa agtgaaactg
     4141 attgctactg ggctcggtga agcacctact gcagtgaaca acgctttaca ctatattaat
     4201 ccaaaggaac gaactcagcc tggtcacagc acaagcttat acgataaaaa tcttcgtcct
     4261 agttgatttt ttatatctct ttgcatttca agttaaaccc gaaagcagtt tcttgcattt
     4321 ttttcatgtt ccgttaacat gaaatagaga aagaaaaaat aaagggggag caataatatg
     4381 gtcgtcaaaa ctgagacttt agaagaaaag gcaagagaac tattgaagga acgtggcgta
     4441 actgtagaag atatcgcaca acttgtcatg tttttacaaa aagactatat ttctgatttg
     4501 actttggcgg attgtactga aagtgtcaac agcgtgctaa caaagcgtga agtacataac
     4561 gccattatca ctggaatcca attagatata ttagcagaaa agaaaaaatt gatgagtccg
     4621 ttgcaacata ttatcgaaga tgacgaaggc ctttacggta tcgatgagat cctagccttg
     4681 tcaatcgtga atgtttacgg tacgatcggc tttacgaatt atggttatat cgacaaggtg
     4741 aaacctggta ttttaaaaga actaaacagc cacgatggcg ttcatgtcca taccttctta
     4801 gatgatatcg tcggagcgat cgccgctgct gctgccagtc gattagcaca caaagaacca
     4861 gaaaaaagaa gtcgccttac tcaataattc aacaaaaaaa gaaagaaaac gtctatttag
     4921 acgagttctt tcttttttgt ttgtttcaag ctattttttc ctaagaaaag aaaaacagca
     4981 aaaaataatt ctaataatac aaaaaataag ataaagctat gcatgaaaaa ttcttctgga
     5041 agtacattga tgattggatc agtaacggga tcgaataacc agtcatcgtt gttgaaaaac
     5101 gtttcatgaa aaaggataaa aaagcgatca aatcctatca tcatgaaaaa accgaaaaca
     5161 actggcagta acatgccgat ttgaaacgga cgaatcaacc gccaaagccg atcattcttt
     5221 tgcaaatatt tgataaacaa aatactcggg atcaaagtga tgaaaaacac agcataatcc
     5281 actaaaaaca aaattttcac ttcataaaag tgatgggctc cgctagcaga aactgggaaa
     5341 tctggcaagc tgaggatggt tttgaaggga ttattgagat aattcattag gtgatcaaaa
     5401 tttttcagta acgtctcttg atctaaagaa gtataatcca aaatttgcaa atgtccgata
     5461 tcaaatacgt atagtggtcg aaagttgatc gtcacaccaa ctgccagact gatcagtgtc
     5521 agaaataggc tgaacaatcc agcatattcc atccactgcc atctcgaatt tttcatatta
     5581 gaagctccat tcatccagtg attgtttcac ataggtagct gggataggca acccaggtac
     5641 atcttcttct tttgtaaaac ctgataaaac gagcaaagta tctatattat tgcgtatccc
     5701 agcttggata tctgtttcat agttatctcc aaccatgatt acttcttctt tttgcagccc
     5761 taatacttca acggctttat ccataatgat ggcttctggt tttccgatgt agatcggcgg
     5821 cgtttgggtt gctgtctcta caaaagagat gactgaccct gctccaggaa gaaggccgcg
     5881 ttcagtaggt atattcttat ctgggttcgt tccaatgaaa gtagcacctt tttgaatagc
     5941 aagtgttgct ttaactactt tttcataggt cagataatta tctaagccta ccactacata
     6001 gtcaggagcc tcttcttccc atgtaaatcc ggcagctaaa atcaagtcaa tcaatcctgc
     6061 ttctccgata acatagactt ttttcccttt tccatctgct ttcataaaat cgattgttgc
     6121 caaagtagct gtgtagactg tctctggagc tacatgaatg tcaaactcat ctgctagacg
     6181 attagctact gtttctggtg acttagtcgt gttattggta acaaacaaga aaggtaattt
     6241 tcttttttgc aattcttcca caaatcgttt accggccggt atgggttgtg tcccacgata
     6301 aatcgttccg tctaaatcaa ttaaatatcc tttatagtcc attcatccac tactcttccc
     6361 tttttctgat cgtaaatgct gtttttgttt tttctacttg tttaggttgt tcttgttttt
     6421 ttactttttc agaggttttt gctgactctg tttttcgatt tttaataacc ggttttttgc
     6481 gattagatgc gactggtcct tttttctcat cgatatacgc acgattatgg ttttttttct
     6541 tttttcggcg ctgagttgtc ttttcttttt taccgccgat tctctcgatg acaaaatacg
     6601 cacaaccaaa attgcaatat tcgtaaagat agtcttccaa ggaatcgatt cgctgatctg
     6661 gatgcgcccg tcgattatcc gcttggaaaa atccttttag gcgtagttgt tcataacccc
     6721 aatctcctac gatataatca tatcgggcta gtacatcact gtaacgttct ccaagctttt
     6781 ctgcattaaa tccttcacga taatcggaca caagccgata atgtctttca cctatcaaaa
     6841 aattactgtc atctaacagt gtcactagct cgcctttgac tttttctgct ttaggctcct
     6901 caacgatttc ttccaaagca gctgtcagtt cttcggtgac tgttgtttcc tgttcagctg
     6961 atgatttttt cttagcaatt tctgttttat gctcaacgtt ctgctgttca ttttgttttg
     7021 cagacatgcg ttcaccacct atctttttta ttataccttg tttttatttg attgggtagt
     7081 gagctttcaa gtaatctcct accacacttc ggataaattc agggaacaaa aattcatttt
     7141 ttccagcaat ctcaatcgtt ggaaaaaatg gaacaaacat caaatgatct acggtagtaa
     7201 aagtataatt tttattcaga tcgattggct tgttcttcca aaagacttga tggttaatag
     7261 aatcatatgt gattccatta tagaccatct gcccaaaaat cttaccgcga aatcccattc
     7321 ccttcatagg atagttacgt aaaaagttac gatttttttc tatttctaaa actaagcgga
     7381 tcagatcatt tccacccaat gtgacattta acaagtgcat cggatgcggc aacgcagtgt
     7441 gaagctgatc ctgattgacc cgcccttttg gaatttcagt taaaaacaga ccactgttta
     7501 atattgctgc ttccgtcttt cctcgttctt tcaccgcctc aaaggctact tggataaacg
     7561 aatgctcatt gaagatatct agactcagat aaaatggcaa atctgctact tctttttctt
     7621 ttaataattc atgcccacgt tctagataac tatttatttc ttgttcatct tctgtaaaag
     7681 ctgtcattgt ttcggtaggg atggctttgg ctgaatgctt aaggattttt ttctcttcat
     7741 cgatggttaa atggacttca ccgacatact gcccgtactt tccagcggct gctatttgga
     7801 catcattgat tacgtgacct tctctgaata aatgatgggt gtgcgatcct aaaataacat
     7861 caattgctgg tatttctttt gcaatttgga aatcatcctg aatcccgaga tgactcatca
     7921 aaactaaaac atccacttgt gaacgtaaat cttcgactaa ttctggcaaa atatcataag
     7981 gattccgtat gtcccagcca ttcggattat aagtaagcgg aaatggagca gtaaatgcaa
     8041 ttagtccgat tttcgtctgg tgtttcgttg taatgatttt atatggtttc gtccatgccg
     8101 gtggctggag cgtattttta tcgaataagt tatccaggat gatatcaaac tcagcatgat
     8161 cgtataaatg attcaactct tctttggaat ttcctacgcc ttcattgttt cctatcgtcg
     8221 ctgcatcata accaacttca ttcatcagca agatattttc ctgtccattc gttgcttctg
     8281 acaatggatg ccaacgatcc acaaaatcac ctagatctac tgcaatcgtt gtcgtttttt
     8341 ctttcttttc gttttctcgt ttacgttgat ccaaaaacct tctgatcctt ggccaatttt
     8401 ctaaatgaga atgcagatca ttcgtatgta aaatcactat tttttccatg taccctctcg
     8461 cctctcttta tctttatcat accataggca gctttttctt ccattgaaac aagctctatt
     8521 tcagagtttt tcctgccttg aagcaaattg tataagtaca atattcgata aatcagtctg
     8581 gctaaaaaat caggaacatt catttttaca gtactataaa aataaaaaaa acagacccga
     8641 aagtctgttg tcgtttcatt ataatcagac acatggcgga acctgcttag tgctgcttcc
     8701 ttccagacct gacacgcttc acaagcccac cattgtccta gcctttcggc aaagaatagt
     8761 ataccttatt tttcattcgt tgaccagtct ttttttaatt tcttttttcg ctggcggagc
     8821 tttcggaaaa agtcagtcaa aatcatccca cattcgtctt ctaaaatgcc accttctaca
     8881 tatgcttggt ggttaaaccg ctcgtcctcc aaaagattca tcaaggtacc ggctgttccg
     8941 ccttttggat catacgcgcc gaaataaact tctgctacac gagacaaaat catcgcgccg
     9001 ctgcacatag gacaaggctc taaggtaaca aaaagtgcag catcttccaa gcgccagctt
     9061 cccacttttt cacaggcttc cttgattgca agcatttctg catgtgtagt agcatcttgc
     9121 gtggtttctc taaggttata tcctcttcca atcacttttc catccaatac aaccactgct
     9181 ccaatcggaa cttcatgaag catttctgct ttctttgctt ctgccaatgc aaggcgcatc
     9241 catttttctt tttctactgc atcaagattt gtatttacca attacccttc cctgcctttg
     9301 ttttttcttg ttttacttta gcatatgata aaaaggctgt aactaacttt tttttctatg
     9361 gtaaaataaa tacaagaggt gaacaagatg aaaatggata aattacttgc tctttaccca
     9421 caagcacaga aaaaaaactc tccagcatca gaagcggata tcctttcttt tgcaattgat
     9481 gatgctttta tctggatcaa acaagattca ttatctcaac aagaaaccag ccttttgaaa
     9541 gcgttattcc ctgtaatcaa cgatcaaaaa aaacacccat ggtatcgcta tctttttaaa
     9601 gatgcttctt gtgttcaaga aaaaagcttt cgtttgatcc agcttcacgt cgaatcaaaa
     9661 ggagaattct taaaaaaaga atggcaagat actattttgg aattatttcg ctattctgct
     9721 gatttctttt tttatacaga cacagatgcg ctcttagtcg aacaaataaa ccgtgattat
     9781 cttgatggta ctgaaatcaa cggtattttt ctatctttag atgctgattt tgatacgaca
     9841 acgtctgtat tcgttggttc tttccattca cctggcaccg acctcgtccc tttgtttgct
     9901 gaagaaagaa aaattttttt aacagagcaa aacaatcttt atactcacag ccgtactttt
     9961 tctatgcaag atgtggcttt acattactat acaaaagata ctgtaaaaga tagtaagttg
    10021 atcaaaagtt acatagaact gatcaaacaa aatgaaatgg aagtgatcat tcaagaatta
    10081 tggaatgaat taggcaatgt cagctctgct gcaaaaacac tgtttcttca tcgtaatact
    10141 ctaaaatatc gaatcgaaaa atttcaagaa cagtctggtt tcaatctaaa aaaagccaac
    10201 gatttactgt tttgctatct tttattgctt catgaacata attaaagata gattaaaggg
    10261 gttgtgacaa taattttgtc acaacccctt atttcgaatg aatagtgttc aaaagctagt
    10321 ttctcttaca tcattttttc aacaaaatgc attagccggt cttttacatc ttcggttgaa
    10381 tctttgattg aatcaccata aacagagatt ttcttttttc ctgatttgat ttttgctaac
    10441 attcccatca aagaatccaa gtcactatct gaaagaccat ctacgatttc tagtaatttt
    10501 tcatttccat caaatttgtc atagacgaat ttttttactt tcgtccgagt agtcatatga
    10561 tggatctttt ccattacttt tcccgataca acgactgctg tcgctacact ggcagcggct
    10621 gctacgctta atccgattgt tacttttgtt gatgttttca taagtatcag ctccttttta
    10681 tctatctcca tcatagcata gacaaaaaaa taaacgaact aatccactca gtgaaatctc
    10741 aatcctttag ggaaaaagtt ctttactgtt ccttttactt gtttcccaaa tccgacaggt
    10801 atcttatcta atactagcaa aacccatcct tgattcccat cacgttgaaa tgtttctcct
    10861 gctgtgtaag attgccattc cttttgagtg atcggcaagc aaggtatatt ctctatcttt
    10921 tttgtagcaa gcgctaatgc atagctaggt tcaaatcgat tctttttgaa atctcctaaa
    10981 tgcaaacctg tacgtacgac ttttaaacca tcgagagaag gggctaactc aggaacttcc
    11041 caaagatggt cgttgaacac taaaagtctt ccagtagctt cataatgaaa atcatttgaa
    11101 aactccgtcc agagtttttc ctgctctttt gtcatttgca ctttactttt ttttcgtgtc
    11161 ttcttttctt tatgcatctg attttgtcca tgaaaagtca gctttgcaac aaaatgtcct
    11221 tcaccctgat ctttatgagg ccatatacgt attgtttttt ccaaaccagc tacgcttccc
    11281 cattcagaac gtcctgaact aacggattga gtcaaaggaa tctcctcaat cgttactgga
    11341 taattttcta caagccagct aatgatctct tcattttctt ctggtgcaaa cgtacaagtc
    11401 gaataaatca gttgtccttt attttttagc atttttattg ctgaagacaa aatctcttgc
    11461 tgacgttttt gacagtatag cggagattct tctgtccact cttttattgc atttggatct
    11521 tttcggaaca ttccttctcc tgaacaagga gcatcaacca cgatccgatc aaaaaagccc
    11581 gaaaaatgag ggacaagttc tgcgggtgca tgatttgtca ctattgcgtt cgaaactccc
    11641 catcgttcta tattttctga taaaatcttc gcacgtttcg gaaagatctc attcgtcact
    11701 aataaccctt tccccttcat ttgagcagct aattgggttg atttaccacc gggagccgca
    11761 cacaaatcca aaactttttc accgggtttt gctgcagcag ctgttccaac gatcattgca
    11821 cttggttctt ggctatattc atacccagct tgatggagga aagactttcc attgacagtg
    11881 ccaagaaatc cctcattgct gtagggagcg ggttgtaatt cttcgctatg gtaagtttgg
    11941 accatgtcca atcctgccgg ttttagtgga ttccaccgaa atcctttttt cacgcttcct
    12001 tgttcaagcg cagaaaaaaa atcggatgct tcttcgccta aaagaagacg atattttttg
    12061 ataaattgct gtggtaacgt tgtcgcttcc tccttcattc caatgtttcg ttagacattt
    12121 tacaatcttt tttacgaaaa ataaaggtgg ctgatctact ttttcatgtt gctgcctaag
    12181 gtgactgttc ctttaagata ttttccaagt attcatccgt cacctcgttt ttatcccaaa
    12241 cagcgttcat catttcaaga aaatcttgag aagaatcatc tgagcgcaaa cttcccaatg
    12301 cattaagcca taccaattca agcgactttt cttctttgat cttcgtgtga cctttgacat
    12361 atgagaaaaa tggccgctta aatagcgaat ccatgtctaa atcagatttt gcaatgattt
    12421 cacgaatact ttcatggata gaaaaaaaat ctttcaataa gtagacaatg cttaaaacat
    12481 catcggaact gcattcagct aagtagtttt caaaataaac agtgatttct tctaatatct
    12541 tctcttttaa cccgctgatt gatttgaaat gtgtataaat cgggtatgta gaacaaccgc
    12601 agaactctgc taactctctt gcagtaattg acttcattcc atattcttcc acgtatacca
    12661 tacttttgaa cagcaaatat tctttactat aatttgttac tctccccatc attcttacct
    12721 ccgtctcttt tcatctaccc ataaataaat aacataaata attctaaaaa tattattgat
    12781 aattatcaaa acatatttag ttatatccat taaaaatcaa tttttccgcc ttaaaaacaa
    12841 gaatatcttg acaaaatacc ctaggaaata aacaatagaa taaacaaggt aacaaaaaaa
    12901 ttaaataacg ttatataaaa aagaaggcga cactctgtga gaataacaca gactgaaatg
    12961 actttccact cacagttttt gtctaactat atacaaaaac agaataaaaa ggaggtattt
    13021 atgctaattg aagcaagaaa aaaagagtta cttgttctac aaatggtcat tcaaaaaaat
    13081 aattgttctt tacaagaatt atccaatgac ttacatattc caaaacggac aatcaaagaa
    13141 ctgatccgaa aaataaatgt atcactcaag caatttttat tgattgatga atttatttat
    13201 tctaatcata aaggagagat ccaaaccaaa gaaaccgatc aaactaaaaa gttagctgtt
    13261 ttttctaagt taaaattatg ctacctaaaa gaatccaatc gttttaacta tttgatgtta
    13321 ctaatcaatt atccacaaac tgctgtacca aaaaaatatt tacttgaaca actttatata
    13381 tcgccctcct atctcgaaaa actgacaaaa cagctcaatc attccttaaa aaaattccat
    13441 ttacggatcc tatctgtaaa aaattgctgt actcttgaag gaaatgagtt atctatccgt
    13501 ctctatcttt acttttttct ttcagataca tttggaggta acgattggcc attttcctca
    13561 cttgaattaa atgcactaaa aatgaacagt acagatgagc aaatagaaaa aatttcaaaa
    13621 caagaaaaaa atatctactt gctgttaact ttattcatat tacgaaacaa acaacaggca
    13681 tattttccaa aaacttcttc tgaaatcact gaattgatgg acttattgca gcatacacat
    13741 gattattcag aatactttac ttctttttca gtgagtcgcc cttgtgaaga gacagaaaca
    13801 gagagagcct actttaatta ttttttctat tgtttttttc caaatcaaat ctcttttcga
    13861 caaaaacagg agattggttt tttattttcc acaagcaaaa accttatttg tcgacaagtc
    13921 ggtcaattga tgacttactt aactcaacag tttcctctca accatttaga aaataaaata
    13981 ccaatttatt tttattttct tgtgttggca gttgtttttt tcgaactgtt gggtgaatcc
    14041 agcagtagtt tttctacact ttgctttcca gaagccacgt ccaatcattc tagttctttt
    14101 atacaggaaa agaaattaag agaaaaaatc gatacaattt tttcaaaaaa tggggaaaga
    14161 aaccaaatca gtcaatacct tagtcaattg atccaacact tactacaaac agaaacgaaa
    14221 gagcagttac tcatccattt gcagataacc aaacagttta caggaaatta tcatcttcaa
    14281 agccatttgc agcagttatt tcgcaaagat gctgttaaaa ttaccgcata ttcagaacat
    14341 gctgatttga tcattacaga tcagcatgag aaaatttcag atgaaacgga gactttctat
    14401 ctggatacaa cgcacaatac agtgttattt ggcaagttaa taaaagcggt tcatgaactg
    14461 tatatgaaaa aaggttatga gaacaaaaca aagaggatgt gacaaaattc ttgtcacatc
    14521 ctcttattc
//
LOCUS       DAFGZE010000072        14201 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.074, whole genome shotgun sequence.
ACCESSION   DAFGZE010000072 DAFGZE010000000
VERSION     DAFGZE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.074"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            476..1339
                     /locus_tag="LTU54_002304"
     CDS             476..1339
                     /locus_tag="LTU54_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBL8296999.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="LTU54_002305"
     CDS             complement(1468..3090)
                     /locus_tag="LTU54_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8297000.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="LTU54_002306"
     CDS             3339..3860
                     /locus_tag="LTU54_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBL8297001.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LTU54_002307"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="LTU54_002307"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HBL8297002.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="LTU54_002308"
     CDS             5941..6759
                     /locus_tag="LTU54_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBL8297003.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6861..7058)
                     /locus_tag="LTU54_002309"
     CDS             complement(6861..7058)
                     /locus_tag="LTU54_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297004.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7227..7805
                     /locus_tag="LTU54_002310"
     CDS             7227..7805
                     /locus_tag="LTU54_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HBL8297005.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7900..8037
                     /locus_tag="LTU54_002311"
     CDS             7900..8037
                     /locus_tag="LTU54_002311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297006.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8850..9353)
                     /locus_tag="LTU54_002312"
     CDS             complement(8850..9353)
                     /locus_tag="LTU54_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL8297007.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9712..10737
                     /locus_tag="LTU54_002313"
     CDS             9712..10737
                     /locus_tag="LTU54_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL8297008.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11060..11938
                     /locus_tag="LTU54_002314"
     CDS             11060..11938
                     /locus_tag="LTU54_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HBL8297009.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12414..12530
                     /locus_tag="LTU54_002315"
     CDS             12414..12530
                     /locus_tag="LTU54_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HBL8297010.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13458..13796
                     /locus_tag="LTU54_002316"
     CDS             13458..13796
                     /locus_tag="LTU54_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297011.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13890..14120
                     /locus_tag="LTU54_002317"
     CDS             13890..14120
                     /locus_tag="LTU54_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297012.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
BASE COUNT         4505 a         2374 c         2717 g         4605 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaata tatatgatat
     6781 atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt atatcataat
     6841 gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc tttgatccag
     6901 cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa ccataaaagc
     6961 ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag cactaaaaga
     7021 accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc tcttgttcat
     7081 tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac tgaagactga
     7141 tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt atcatctaaa
     7201 atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca tttacagatg
     7261 ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct aacggagaca
     7321 cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc agttttatca
     7381 ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat aagataaatg
     7441 gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt ccgtttgcaa
     7501 cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat ggttttgtta
     7561 ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa gcaaataaag
     7621 gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat ctatcgattg
     7681 gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc gtgctgatcg
     7741 caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa tcacaatatc
     7801 ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa ataaacgatc
     7861 aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa aagaattcat
     7921 gtaggactga ttactagttt tgtcacgatg cttatttatt tggatcaagg tcatttgact
     7981 tggacagtag ttggtgtcgt aggatttttg acattattca cagcaataaa aaaataaaca
     8041 tctttatgta atctgatgtt ctatctattt aagaaaagca tgtttacagc agtatgattc
     8101 tgttgtaaac gtgtttttat ttgtggaaaa tttaattttt agaaaagtgt atcgaaaaat
     8161 agatagttag tttttccaca gtggataact tcgtaagaga taaaaataaa aaattagtca
     8221 aagaagggcg tatagatttc ttttgatagg agatatacaa cagaaatgaa taaatattaa
     8281 cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa catatctcat
     8341 tttacacttt tgttatttta tacttaaaaa ataacgacac agaaaatacc gtgtctcaga
     8401 tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg
     8461 atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca
     8521 tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga
     8581 gtagggtata aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt
     8641 taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc
     8701 ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa
     8761 tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg
     8821 tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat
     8881 aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct
     8941 aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct
     9001 cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga
     9061 atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt
     9121 ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg
     9181 gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca
     9241 ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac
     9301 cataatagaa agaataaata aatccctaat aaaaattttg atacataccg catagacatc
     9361 tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt
     9421 ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt
     9481 ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa
     9541 aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact
     9601 aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg
     9661 catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag
     9721 acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta
     9781 cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt
     9841 tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg
     9901 aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg
     9961 acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc
    10021 gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc
    10081 atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt
    10141 atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct
    10201 gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc
    10261 attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg
    10321 aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac
    10381 ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt
    10441 gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact
    10501 tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt
    10561 cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg
    10621 atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg
    10681 gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag
    10741 gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag
    10801 gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac
    10861 tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg taactgaata
    10921 aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag
    10981 aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg
    11041 ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa
    11101 aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga
    11161 gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga
    11221 actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga
    11281 ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat
    11341 ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag
    11401 atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat
    11461 ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata
    11521 ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat
    11581 tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg
    11641 tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt
    11701 actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc
    11761 cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg
    11821 acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa
    11881 agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt
    11941 ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca
    12001 cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat
    12061 tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct
    12121 tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact
    12181 agctataaca atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac
    12241 gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt
    12301 taacaagttg actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt
    12361 cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa
    12421 aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac
    12481 gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa
    12541 acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt
    12601 gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag
    12661 aatctttctt ttcaaattga taccattccc taattccagt tatatagtta actacagaac
    12721 cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac
    12781 ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga
    12841 aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc
    12901 atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa
    12961 gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa
    13021 aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc
    13081 cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat
    13141 aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt
    13201 aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat
    13261 taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt
    13321 gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat
    13381 gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta
    13441 gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc
    13501 gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta
    13561 gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta
    13621 tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg
    13681 aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca
    13741 ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac
    13801 tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat
    13861 cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt
    13921 attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc
    13981 gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa
    14041 ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt
    14101 atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt
    14161 attgtttttt cgatatcttt gctgttaata ttactaactt t
//
LOCUS       DAFGZE010000073        13917 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.075, whole genome shotgun sequence.
ACCESSION   DAFGZE010000073 DAFGZE010000000
VERSION     DAFGZE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.075"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            131..505
                     /locus_tag="LTU54_002318"
     CDS             131..505
                     /locus_tag="LTU54_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL8297013.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="LTU54_002319"
     CDS             complement(619..1248)
                     /locus_tag="LTU54_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL8297014.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="LTU54_002320"
     tRNA            complement(1382..1454)
                     /locus_tag="LTU54_002320"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="LTU54_002321"
     tRNA            1668..1741
                     /locus_tag="LTU54_002321"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="LTU54_002322"
     CDS             complement(1879..2043)
                     /locus_tag="LTU54_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297015.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="LTU54_002323"
     CDS             2338..3666
                     /locus_tag="LTU54_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBL8297016.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="LTU54_002324"
     CDS             3868..4701
                     /locus_tag="LTU54_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBL8297017.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="LTU54_002325"
     CDS             4717..5454
                     /locus_tag="LTU54_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HBL8297018.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="LTU54_002326"
     CDS             complement(5609..6577)
                     /locus_tag="LTU54_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBL8297019.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="LTU54_002327"
     CDS             6809..7642
                     /locus_tag="LTU54_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBL8297020.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="LTU54_002328"
     CDS             7676..8515
                     /locus_tag="LTU54_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8297021.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="LTU54_002329"
     CDS             8541..9101
                     /locus_tag="LTU54_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL8297022.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="LTU54_002330"
     CDS             9300..11021
                     /locus_tag="LTU54_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HBL8297023.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="LTU54_002331"
     CDS             11186..12328
                     /locus_tag="LTU54_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBL8297024.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="LTU54_002332"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="LTU54_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBL8297025.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4035 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttg
//
LOCUS       DAFGZE010000074        13814 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.076, whole genome shotgun sequence.
ACCESSION   DAFGZE010000074 DAFGZE010000000
VERSION     DAFGZE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.076"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="LTU54_002333"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="LTU54_002333"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBL8297026.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="LTU54_002334"
     CDS             complement(2262..2513)
                     /locus_tag="LTU54_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HBL8297027.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="LTU54_002335"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="LTU54_002335"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBL8297028.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="LTU54_002336"
     CDS             3463..3681
                     /locus_tag="LTU54_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297029.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3970..4482)
                     /locus_tag="LTU54_002337"
     CDS             complement(3970..4482)
                     /locus_tag="LTU54_002337"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBL8297030.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="LTU54_002338"
     CDS             complement(4496..6793)
                     /gene="recJ"
                     /locus_tag="LTU54_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBL8297031.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6935..7378)
                     /locus_tag="LTU54_002339"
     CDS             complement(6935..7378)
                     /locus_tag="LTU54_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HBL8297032.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7379..8167)
                     /locus_tag="LTU54_002340"
     CDS             complement(7379..8167)
                     /locus_tag="LTU54_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8297033.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8164..9105)
                     /locus_tag="LTU54_002341"
     CDS             complement(8164..9105)
                     /locus_tag="LTU54_002341"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBL8297034.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9282..9869)
                     /locus_tag="LTU54_002342"
     CDS             complement(9282..9869)
                     /locus_tag="LTU54_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HBL8297035.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="LTU54_002343"
     CDS             complement(10090..11397)
                     /gene="obgE"
                     /locus_tag="LTU54_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBL8297036.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11691..12701
                     /gene="gap"
                     /locus_tag="LTU54_002344"
     CDS             11691..12701
                     /gene="gap"
                     /locus_tag="LTU54_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBL8297037.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12786..13721)
                     /locus_tag="LTU54_002345"
     CDS             complement(12786..13721)
                     /locus_tag="LTU54_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBL8297038.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3808 a         3099 c         2437 g         4470 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa atagcgaccc tcaaaagtca gatagagaat
     3901 ctgacttttg agggtcgctt taaaatcgct tctttaagaa taacatcaag aatagcttct
     3961 tttttctgct taatattcca ttaatgtaat gatgtcatat cctttgattt tgtcgcgtcc
     4021 atggagatcc atcaattcga tcaaaaatgc gcagcctacg actacaccgc ctaattgttc
     4081 gatcagttcg atcgtcgctt tgattgttcc tccagtagct agcaaatcgt cacaaatcag
     4141 cacacgttga cctggtttaa tggcatcctt atgcaatgtc aatgtatctg ttccatattc
     4201 taatccataa gtcacttcga tggtttctcg aggtaacttc ccttttttgc ggactggggc
     4261 aaaaccgact cctaactcat aagcaacagg acatcctacg atgaatccac gggcttctgg
     4321 cccaactacc atatcaattc gtttttcttt tgcataattt acgatttgtt tagtagcttc
     4381 ccgataagcg tcaccatctg ccatcagtgg ggaaatatct cgaaacacaa ttccttttga
     4441 cggatagtcg ggaatactgg caatataatc ttttaaatcc actctttgtt tcctcctagg
     4501 ttgttaacca tttctttatg gtaggaatat cgctcaaaag caaaaattcc tcaactttga
     4561 ttttattgag ccgtcgctgg tataatctgc tctcagtcaa aggatgactt tctgggctag
     4621 cgactttttg taaaacaccg ttctctattg taacaaattt caattcaaaa aacacctgta
     4681 tcataaaaat taataatttt tcagggatac gcaaatattg agctattgct tttattttat
     4741 gacgtatatc aattcgttct tgagaatgga tcaatttaaa caattttgcg tactgttctc
     4801 ttgtgcctac cccattcaaa taggcctcat caggtgaaat ccccatcaga taaacacgat
     4861 tgaatgaata agaccgaaaa acttctttca gatccgctaa atcatctgga caatccataa
     4921 caactagaga gtttggtttt cttttttcaa ttattgcctt tacagaatct agtccttcga
     4981 aaacaatcgc agcttcctgg ataatctcag gcacgaattt caacgtcgaa gaatcaaatg
     5041 ctaaataaag gctaccttcc gacgttcggc tctgttcacg gaaacgtttt gcccgccaat
     5101 caaatacttg cagaccaccc acagaaaaat cagataccat cagctgcggt ttcttacgcc
     5161 cgttccattc attgatcgaa agctttccta ccacatctac ctgattattc agcagttctt
     5221 cttcttgagt accaaaacca aaagcgaccg catcaagctg actcgatgca tcggaaagga
     5281 caaatttcag atgctgttgg tttgctccga ttcttttgat attttctgct tggacttgtc
     5341 gaaataaaaa ctgaggcaaa ggattatccg taccaaatgg cgccaatagt ttcagctcat
     5401 caattcgctc tactgttact tcatttggca gcaatacttc atcaattctc aactcaggcc
     5461 ctctcatcaa gtcaatttga tgatcgacta tatattggtt catcttctcc tgtagtatag
     5521 tgacattttc acttggcatc gttaaaccga ctgctgcatg atgtcctccg aagtaagtaa
     5581 ataaatctcg catctggtca agcatttcaa aaagattcaa ggcttcaata ctacgtccag
     5641 atcctttagc tgatccatct tcttttaaag taaggacaag cgttggttta cccgtttcat
     5701 tcattatttt cccagcaaca atgcccaaaa cgccttcatg ccatcctcgg tttaccaata
     5761 gatggatatg attctcctta ttgaccatcg ctaatgcttc ttgtgttatc tgctctacga
     5821 ttgcttttct ttcttcattg atctcattca atttttttgc ttgaacggat gcttcctctt
     5881 catcaaacgt cgcaagtaac gagacggcag gacgtggatc gcccatccga ccgattgcat
     5941 ttagtctggg agcaatcgaa aaaccgatcg ttgtttcgtc tgcgtccctc atttttacgc
     6001 cgctttcttc aaatagcgcc tgaagtccta tccgttctga atgatgaatg gcatcgattc
     6061 ctaatgaaac aaggattcga ttctcatcag tcaatgaaac taaatcggcg atcgttccta
     6121 tcgctactaa atccaaaaat tctgcaggcg gttcttccaa taatgcagaa gctactttga
     6181 atgctactcc gactcctgcc aaatctggaa atggatagtg ccccgctgga tgacgagggt
     6241 gaacaatcgc ataagcgtct ggtaaattct caggtagttc gtgatgatct gtaatgatca
     6301 cgtctacacc agcttgctga gcgtatgcta ccgcatcatt tcctgctacc ccgttatcaa
     6361 cagtgatgat caactggatt ccttcttcga tcttttcttg gtataccgat tggtttggtc
     6421 catacccatg ctcaaatcga tttggcagga atacttctac ttctgcacca agtaattcca
     6481 gtgtttcttt cattaccgtt gcactggtga tcccatctgc gtcataatca ccgtaaacga
     6541 ggattttttc tccactgatt acagcttctt ggattcggct gattgcttta tccatatcgt
     6601 gtatcagata cggatcatgt aattcttgtt cctttggatg taaaaatcgg ttgatatcct
     6661 tttcctcatg gtaacctctt tgccacagta acttaccgac aaatggagat aatttcagtt
     6721 cacggatcat ttggagaaag gattcagaag gctctgtctc gcttaacttc caatcaaaat
     6781 ttgcttgtct cactttctca ctcctaactt agttattata gcaaaggaaa tcagcaaaaa
     6841 aaagcagaaa gaaagaataa aaacagatca gatccgctag acagatgttc agcaggacct
     6901 gatcttttgt acgaccattt atgaaatatc cctattagtc aaaataattg aagctttgtt
     6961 tgggcgattg agttttttgt tcttccggcg ctttcaattg actgatttgt gcggttttcg
     7021 cttgtaactc cgcctgataa gcagcttcca actccgcacg ttcgtttcta aactcgcgtt
     7081 gctgttcttc ttttgcttct tcaattttct tttgaagttc tgtttgatat tctgacattt
     7141 cctgagtcag ctgtttgatt tgcttttttt gctgccatag tgttgccgaa gcagtcaaaa
     7201 atacaatcag tgcaccaatc agcgcagaac cgatgatcac taagatcaat ggcgcagaaa
     7261 aggaagtgaa gccaaaactt acaggaacat ttttattgtt caatacagca aaaagaacaa
     7321 taatcaaaac taaaacaaat ccaagaatca cattgctttg ctttttcaat tccttcacct
     7381 actttttgtt aaaaattccg ctggctaaat aatcaccaat cgttggaaac agcgtataaa
     7441 attttgatgc aacttctaaa ataaacggtt gattgatttc ccgttttgga tgtctcatat
     7501 tgcggacgat catttttgcc aacttggcag gttctaaaac gatttgtccg actttttcca
     7561 aatagcttcc gcttggatca gccttatcga agaaatttgt ctcgattgga cctggattca
     7621 ctgtcgtaac agctacgcca agtggtttca attctaatcg caaagcgttg gagaagccaa
     7681 ggactgcaaa tttcgtggca gaataaaccg ttgattttgc agttgccatt tttccagcca
     7741 tagaggcgat attaatgata tgaccttgtc ttctttctgc catatcgatc gcaaattttt
     7801 gtgtcaaaac catcattcct aagatgttga cttcaaacat atcgtatgct tttccaaggt
     7861 cgaatgtaag gaagtcttcg aaaatcccaa aacccgcatt gtttaccaga atatcgacgc
     7921 gcccgacttc ttccatcacc ttttcatata attcgtccac gctttcagga ttgctgacgt
     7981 ctaccttgaa agcataagcc tctttgccgc tcaagcgtct gcactcgtct ctgacacctt
     8041 ctacaaatgc cattcttcgt gcgcaactta taacgattgc tccttgcttt gctgcttcat
     8101 aacagatttg tgcgcccaga ccgcctgaac tacctgttac taaaacaact ttatcttcta
     8161 aattcatgcc tcttcctcct cgcgaaatgg aatctcaatg ctatcaaaat ctttcataat
     8221 cgtcgtgttt tcaaacactt cttgtgcttc tttttcaagc tctaaagctg cttttcctaa
     8281 atatctcgca ctgatatgag taagtagcag ccgttttgcc tgcgcttctt ttgcaacttc
     8341 agcagcttga tgagtcgtgg aatggaaata gttgtgcgcc attcttgctt catctttatt
     8401 aaatgtgctt tcatgtacca gaatatcgct attttccgca agcacaacgg aattatgtgt
     8461 ttttctcgta tcgcctaaaa tagtcacgat tcgtcctttt ttatctggac ctacgaaatc
     8521 ttttccattg atcgtccgcc catcttctag ttggatcgtt tcaccttgct tcagtttgcc
     8581 gtacagtggt ccagctggaa tacctaattc ttttagacga tccacctgca attctccttt
     8641 atgatcatgt tcaaccacac gataaccaaa actgtcgatc ccatgattta gtttttttgt
     8701 ataaacagaa aattgctgat cagtaaaaat cggggctgtt tcatttagct caatgaattt
     8761 cagcggataa cttaaacgcg tttgcgaaat acctaaagag acttttatgt attcctctat
     8821 cccttttggc ccgtaaatct ctaatggcgt atccccacct tgaaatgacc ggctgctgat
     8881 gagcccggga agtccaaaaa tatgatcgcc gtgtaaatgt gtaatgaaaa ttttaccgat
     8941 ttttctcgga cgaatagtcg ttcgcaaaat ctgcatttgc gtgccttcgc cacagtcgaa
     9001 caaccaaact tcattgcgct cgtctaaaag tttcaatgct atacttgtca catttctatg
     9061 ttttgcagga acacctgcac ctgttcctaa aaattgtaat tccataatct acctgacttt
     9121 ctttaagttc ctctttaatt ttagggtaaa aagaagggat aatcaaccgt aagtcgagaa
     9181 tgggttcaaa ttaaaaataa cgttctaaag cgatagcgtt ttctttgaaa attactataa
     9241 aaacagcagc gaacccgttt gagatcctct gctgtttttt attaatttga agatccagaa
     9301 ctttcattgt cgttggaagt ttgcggatgt ttgtccttcc aagtcttttg tgcagcttct
     9361 ttcgcatgat tttcagcttc tttcgcatca tgcacatctt tttcgacatc ttcataatcc
     9421 aagcgagtaa tttctccgcc taattccatc attaccaatt gattcagtgg gcgattgtcc
     9481 tcttcatcag caatcaagat cagtcctgta gctccttcac cgattttatt tcctacaaag
     9541 tcgaacacgg tctgggcatc ttttatttct ttggcatctt gtgtcgcacc gatcattcct
     9601 ccggcaaacc atcctaataa aatccctagc ggaccaccta atacaccaac caacattccg
     9661 attaatccac ccgtagaaga tttgttatta ccggtaaagt cgataaaatc ttctattttg
     9721 aattgatgtg tcccattagg atcatggctg acaactgcca tctgttctcc tttaatcgaa
     9781 cgaacagcat gtaacttttt gatttctgaa aatgcctgat atgcctggct ttctacttga
     9841 aaatttaaaa tgattactcg tttgctcatt aaaaacgccc ctttcatacc ttcttgttat
     9901 ttattttacc tttaattgaa aaataagaaa agaaaagcac tctttttttc caataaaata
     9961 gggctgtgac aaaagtcgta tcacagctcc tttttccgaa taaacggcgt tcttgaagct
    10021 gatcacttct gtcacaacct cccttaaatc tgaatctata ttttttggta tttgttttta
    10081 tttgcttgcc tattcgacaa attcaaattc aaactcaccg atacgtacaa ggtcgccatc
    10141 tttcgcaccg cgtgcccgaa gagcttcatc cacacccata ccacgtaatt gacgagcaaa
    10201 acgcatcact gtttcatcat gatcgaaatt cgtcatttgg aataattttt cgatcttctc
    10261 accagataag atccacgttg catctgaatc acgatcgatt tggaactcag gaccttctgg
    10321 attgaatcca tagtgtacag tttcttcttc cagttcttct tcgtacaatg ggaattctgg
    10381 cgttacttct aacaaatctg ctgtagcatt caataacgcg tccaatcctt gtcgagtcac
    10441 tccagaaatt ggaaatactg ggatgtcatc agcaaattca tcttcttttt ccttgttcag
    10501 ctgttctttg aatttcacta gattttccgg tgcatccggc atatccattt tgtttgccac
    10561 gatgatttgc ggacgctcta gcaagcgtaa gttataagta gataattctt tgttgatggc
    10621 aagatagtct tcataaggat ctcgtccttc catcccactc atatcgatca catgtaaaat
    10681 tactcgtgtt ctttcgatat gacgaaggaa ttgtgtaccc agcccaacac cttgcgaagc
    10741 tccttcaatc aagcctggca agtctgctgc ggcaaaactt ctgccatcac ttgtagtaac
    10801 cattcctaga ttaggtacga gcgtcgtgaa atgataagcg ccgatttttg gacgagcaga
    10861 tgaaataacg gatagtaaag tcgatttccc gacagatgga aaacctacaa gtccaacgtc
    10921 tgccaatact ttcaattcta gttcgatttt tcgctcttgt cctggttcac cgttttcagc
    10981 aatttccggt gcaggattcc gaggagaggc aaaacggata ttccctcgtc caccgcgtcc
    11041 accttttgcc acaactagtg tttgaccatt ttcaattaaa tctcctaaaa gagcaccagt
    11101 ctccgcatct cggacagtgg ttccttgtgg tactttaaca taggtatctt ctgaaccacg
    11161 accgtgcatc cctttactca tcccattttc acctggctgc gctttgaaat ggcgattgaa
    11221 ccgaaaatcc atcaatgtac gtaacccttc atcgacgatc aagatgacat cgccgccacg
    11281 accaccgtct ccgccagccg gtccgccgtc aggaacgtat ttttcgcgac gaaatgcaac
    11341 cattccgtct ccgcccttgc cagctttcac atcgatcgtt acttgatcta aaaacatgga
    11401 cataattaag tcctcctatt attcatttat ttatttgact gcctgaattt ccacaataaa
    11461 caaaagtctt acttatttta tcggtgaaat ctgattttgt ctatcatttc agcaaaaaga
    11521 ttgtttacct tcttcttttt ttatcatttg gtaaggtttc caaccttttt tctgctacca
    11581 agtataattt tttgacaaaa tattctttgc gtagcagaat aacagtgtat taattgtatt
    11641 aattgaggat gccgtttcac agcaccccta ataaaggagg aacttttgat atgacagtaa
    11701 aagtaggtat taacggattt gggcgtattg gccgtctggc tttccgccgc atcaaagaag
    11761 tctcagatga tattgaagta gtagcaatca acgacttaac aagtccaact atgttagcac
    11821 aattattgca atttgactct actcatggta cttatcctgg cacagttact gcaacagaag
    11881 actcaatcgt agtggatggc gaagcaactc gtgtttatgc agaacccgac gcaagcaaaa
    11941 tcccttgggc aaaagaaaac ggtgtagata tcgtactaga atgtactggt ttttatacat
    12001 ctgaagaaaa agcaaaagcg catttagatg ctggtgtcaa acgagtagtt atttctgccc
    12061 cagctggtgc tatgaaaacc atcgtctata acgtcaatga tgatacatta gatgaaaacg
    12121 acaaaatcat ctcagcaggt tcatgtacaa caaactgttt agcacctatg gcttatttct
    12181 taaataatga atttgggatc gaagttggta caatgacaac agttcatgcc tacacttcca
    12241 cacaaatgct cttagatgga cctgtaaaag gcggtaattt gcgtgctgct cgttcagctg
    12301 ctgacaatac tatcccacat tctactggtg ctgcgaaagc aatcggctta gttatccctg
    12361 agctacaagg aaaattacaa ggacacgcac aacgtgttcc agtcgttgat ggttcattaa
    12421 ctgaattggt ttcagtattg aaaacaaaag taactgctga tcaagtaaac gaagcaatca
    12481 aaaaacacac agttgacaat ccttcattcg ggtatgatga ccgtcaaatc gtttctagcg
    12541 atgtcattgg aacaactcaa ggttcgatct ttgacccaac gcaaacagaa gtaacaactg
    12601 ctggtgattt ccaacttgtt aaaactgttg catggtatga caatgagtat ggatttactt
    12661 gccaaatgat tcgcttacta gaaaaattcg ctaacttgta atttgataga tcaatcgata
    12721 aaagaagcct ttgactggat ccagtcaaag gcttctttct tttcatcccc tacttttaaa
    12781 ctcgttcaat gaacatattg gcttaaaaat tgttggacta cttgctgata tttttcagga
    12841 tctgtcttgt atgcttccgc atgctcggct ccttttacaa catacttttc ttttggtccc
    12901 ttcgttgcac gatagacctt atctaacatt tcaaatggta caaatgtatc ttcgtctcca
    12961 tgtataaaga gcattgggcg ctggtttttc ttcaattgtt ccactgcatt tgcttcaccg
    13021 aaaaagtagc cagcacggac ttttgtcact agactcgtta cttgcataag cggaaatgct
    13081 ggaagactaa acatatcgtc tagctgataa gcaagctcat catgaacgga agaataaccg
    13141 caatcttcaa tgattgcttt cacattatct ggtagttttt ctccgctagt catcataact
    13201 gttgctgctc ccatgctgac accgtataga acgatttctt gcgattttcc attgttttct
    13261 agcactttgt ttatccactg tacatagtct ttacgctctg gccatccaaa tccaatgtaa
    13321 tccccttcgc tttttccatg tcctcttgca tcaggaacaa ggacattgta acccatatca
    13381 tgaaacatct ttgcgtaatt ggtcatcgtt tcagcgtttc ccatatagcc atgagccaca
    13441 agagctgttt tttcactttt cgtttcagca ggaagataaa tcgctgataa tttcagaccg
    13501 tcttttgaag tgatggtcca atcgcttcgg ttttcttttg aactaaacca tttatgtgca
    13561 tccgtttctt tggatgtctg tgttgtacca ggtgtatctt cgtctaaaaa gtctttctca
    13621 gaaggcacaa cagcataatt ataaaaataa ttccctgcaa acactaacgc aacaatcaga
    13681 gctaaagcga cgcctaaaag gattttcatc caaagtttca taaatcatcc atctcatttc
    13741 tcttttttca tataaccttg aattagtata atcaaagaat ctctactatg caatgttcat
    13801 ttttataacc cgcg
//
LOCUS       DAFGZE010000075        13777 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.077, whole genome shotgun sequence.
ACCESSION   DAFGZE010000075 DAFGZE010000000
VERSION     DAFGZE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.077"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            153..395
                     /gene="yaaA"
                     /locus_tag="LTU54_002346"
     CDS             153..395
                     /gene="yaaA"
                     /locus_tag="LTU54_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBL8297039.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            382..1506
                     /gene="recF"
                     /locus_tag="LTU54_002347"
     CDS             382..1506
                     /gene="recF"
                     /locus_tag="LTU54_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBL8297040.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            1503..3449
                     /gene="gyrB"
                     /locus_tag="LTU54_002348"
     CDS             1503..3449
                     /gene="gyrB"
                     /locus_tag="LTU54_002348"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBL8297041.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            3469..5940
                     /gene="gyrA"
                     /locus_tag="LTU54_002349"
     CDS             3469..5940
                     /gene="gyrA"
                     /locus_tag="LTU54_002349"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBL8297042.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            6176..6475
                     /locus_tag="LTU54_002350"
     CDS             6176..6475
                     /locus_tag="LTU54_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBL8297043.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            6525..7037
                     /gene="ssb"
                     /locus_tag="LTU54_002351"
     CDS             6525..7037
                     /gene="ssb"
                     /locus_tag="LTU54_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL8297044.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            7063..7299
                     /locus_tag="LTU54_002352"
     CDS             7063..7299
                     /locus_tag="LTU54_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBL8297045.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            7442..9415
                     /locus_tag="LTU54_002353"
     CDS             7442..9415
                     /locus_tag="LTU54_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBL8297046.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            9421..9873
                     /gene="rplI"
                     /locus_tag="LTU54_002354"
     CDS             9421..9873
                     /gene="rplI"
                     /locus_tag="LTU54_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBL8297047.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            10147..11514
                     /gene="dnaB"
                     /locus_tag="LTU54_002355"
     CDS             10147..11514
                     /gene="dnaB"
                     /locus_tag="LTU54_002355"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBL8297048.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            12294..13586
                     /locus_tag="LTU54_002356"
     CDS             12294..13586
                     /locus_tag="LTU54_002356"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBL8297049.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         4587 a         2486 c         3024 g         3680 t
ORIGIN      
        1 atctctttta aggctttctg tttatttttg tttaaatcac cttttttaga ttaaatcgtc
       61 ttatatggaa aaatatatgg ataaatttgt tttattgcga aaaaaaaggt ataatagagt
      121 atacttttaa taatgaaaat gagggatggg cattgaaaca acaagtcatt ctaaatacgg
      181 aattcatgac gttgggccaa atgttgaaag aagtcacagt tatcggtagt ggcggacaag
      241 caaaatggta tttggcagaa aacactgtgt tagtcgatgg cgaaccagaa aatcgtcgtg
      301 gacgcaaact ttatcccggc atgatggttg aagttccaga agtaggaact ttttttatgg
      361 taaaaaaagg aaacgacgat aatgaggctg aataagctgt atttaaaaaa ttatcgaaat
      421 tacgaagagc tgaacatcga attttccaag aacctcgtca tctttttagg agaaaatgcg
      481 caaggaaaaa cgaatctttt ggaaagtatc tacgtattag ctatgacgcg aagtcatcgg
      541 acaagtagtg agcaggaatt gattggctgg actgacgatc aggcgatgat tcaaggagag
      601 atcacaaaag gttcatccac tcttccatta gaaatcttat tatctaaaaa aggccgaaaa
      661 accaagatca accatatcga acagaaaaag ttaagtactt acgtgggaca aatgaatgtc
      721 attttatttg caccagaaga tctttcttta gtaaaaggca gcccgcagat caggcggaag
      781 tttttagata tggaattagg ccagatcaat ccaatctatc tttatgatct tgtccaatac
      841 caagcaattt tgaaacaaag aaaccagtac ttgaagcaat tgaatgaaaa aaaacaaact
      901 gatttgcttt atttggatat tttgtccgaa caactagcag cgtttggcag caaagtattg
      961 aaagcaagag cacagttcgt caagcgattg gagttttggg ctaattcttt gcatcagcaa
     1021 atcacccatc aaaaagagca gctggaaatc gagtatctca ccgcagtcga ttctttagaa
     1081 acacatacgc aagagcaaat ccaagaacaa tttctcgcac ttttgaacca aaataaaaag
     1141 aaagaccttt ttcggggaac aacgacagtt ggtcctcatc gtgacgatct ttcctttttt
     1201 attaatcaga aaaatgtgca gacttatgga tcgcaaggac agcaaagaac aactgcacta
     1261 agtgtcaaat tagcagaaat cgatttgatt aaagaagaga caggggaata tccaatcctt
     1321 ttgttagatg atgtgatgag cgagctggat gataatcgtc aactccatct gctagaaaca
     1381 atcgaaggaa aagttcaaac gttcttaaca acaacgacac ttgaccatgt aaaaaataaa
     1441 atgactgttg aacctgagat tttttatgtt caacaaggga aactaaaagg agaagaaact
     1501 gaatgacaga agaaagaagt ttagtagaac gcgctaaaga gtatgatgcc agtcagattc
     1561 aagtacttga aggacttgaa gccgttcgta aacgacctgg catgtatatc ggttcgacta
     1621 gttctgaggg tctgcatcac cttgtttggg aaattgtcga taattcgatc gatgaagtat
     1681 tagcaggttt tgcaacgaaa atccatgtga ttattgaaaa agacaacagt atcaccgtta
     1741 ttgacgacgg tcgggggata cctgtagata tccaggcaaa aactggacgt cctgcagttg
     1801 aaacggtctt tactgtttta catgctggag gaaaattcgg cggtggtggg tacaaagttt
     1861 ctggtggact tcacggagta ggatcttctg ttgtaaacgc gctttctaca cagcttgatg
     1921 tcaaagtata caaagaagga aatgtgtact atcaagaata tcgtcgtgga gctgtagtgg
     1981 acgacttaaa gattatcgaa caaacggatc gccatggaac aacggttcac tttacgccag
     2041 accctgaaat ttttacagag acaactgagt ttgatttttc aaaattggct acccggatcc
     2101 gtgaacttgc tttcttgaac cgcgggatgc gtatatcgat tgaagacaaa cgagaagaag
     2161 aaccagtcat caatgaatac cattatgacg gcgggatcaa aagttatgtc gaatacttga
     2221 atgcaaacaa aaccgtgatt tttccagaac ctgtatattt ggaaggtgaa cagcaagata
     2281 tcgcagttga agtctctatg caatatacag acggctacca ttccaatatc atgagttttg
     2341 ccaacaatat ccatacgtat gaaggtggaa cacatgaatc aggattcaag actgctttaa
     2401 ctcgtgtgat aaatgattat gcccgaaaac aaaagctgat gaaagaaaat gacgacaact
     2461 tgaccggtga agacgtgcga gaaggattga cagctgttat ttctatcaag catcctgacc
     2521 ctcaattcga aggacaaaca aaaacaaaat tgggcaattc cgaagtacgg acagtcactg
     2581 atcggttatt tagcgaacat ttcatgaaat tcttgctgga aaacccaagt gtgggccggc
     2641 aaattgttga aaaaggatta ctagcttcca gagctcgtct agcagcgaaa cgtgcgcgtg
     2701 aagtaacgag acgtaaaggt gctttagaga ttagtaatct gccaggaaaa ttagcggact
     2761 gctctagcaa cgatcctgaa aaatgcgaat tgttcatcgt cgaaggagat tcggccggcg
     2821 gatcagctaa gcaaggtcgt aatcgagagt ttcaagccat cttgcctatt cgtgggaaaa
     2881 tcttgaatgt tgaaaaagca agcatggaca agatcttggc aaatgaagag atccggtctt
     2941 tatttactgc tatgggtaca gggtttggag aagattttga tgtatcgaaa gctcgctacc
     3001 acaaattagt tatcatgact gatgccgatg tcgacggcgc gcatatccgt acattgctgc
     3061 tgactctttt ttatcgttac atgcgtccta ttgttgaagc tggatacgtc tatattgctc
     3121 agccaccttt atacggggta aaacaaggga agaatattac gtatgtacaa ccaggaaaaa
     3181 atgcagaaga agagttgaaa caagtcgttg cttcacttcc tgcctcacca aaaccaagcg
     3241 ttcagcgtta taaaggactt ggagaaatgg atgatcatca actatgggaa acaactatgg
     3301 acccaagcaa tcggatgatg gcccgcgtca gtgtggacga tgctattgca gcagatcaga
     3361 tatttgaaat gttgatgggg gatcgagtag aaccccgtcg tgcattcatc gaggaaaatg
     3421 cacactatgt gaagaactta gatatttaat catgaaggag gaaaaagaat gagtgaagaa
     3481 atcagagaaa acatccatga tgtcaatcta accagcgaaa tgaaagactc cttcatcgat
     3541 tatgcgatga gtgttattgt cgcacgtgca cttcctgatg tcagagatgg tctgaaaccc
     3601 gttcatcgcc gtattttata cggcatgaac gaattaggtg taacaccgga taaagcccat
     3661 aaaaaatctg ccagaatcgt cggagatgtc atgggtaaat accatcctca tggagacatt
     3721 gcgatctatg agtcaatggt acggatggca cagccattca gctaccgtta tatgctagtg
     3781 gatggtcatg gaaactttgg ttctgttgac ggtgacggtg ccgccgctat gcgttatacc
     3841 gaagcacgga tgagcaaaat cgctacagaa atgctgcgtg atatcaataa gaacactgtt
     3901 gatttccaaa gcaactatga tgatacagaa aaagaaccag ttgtactacc tgctcgcttc
     3961 ccgaatcttt tagtgaacgg aacaacgggg atcgccgtag gtatggccac gaatatccca
     4021 cctcataact tgtctgaggt agtagcggct atcgatttgc tgatggataa tcctgatgtg
     4081 acgaccaatg aattgatgga agtattacct ggaccagatt ttccaacagg tggtttggtt
     4141 atggggaaat caggaatccg aagagcgtat gaaacaggta agggttctat taccgtacgt
     4201 gcaaaagttg agattactga gatgccaaat ggtaaagaac ggatcatcgt gacagaattg
     4261 ccttatatgg tcaataaagc aaaattgatc gaacggattt ctgaactgca cagagacaag
     4321 cgtatcgaag ggatcacaga tttgcgggac gaatcttccc gtgaaggtat gcggatcgtc
     4381 attgacatcc gtcgtgacgc cagcgcatcc gttatcttga ataacttata taaattgaca
     4441 tccttgcaaa attcatttgg attcaatatg ttggcgatcg aaaaaggtgt gccgaaagtt
     4501 ttgagcttga aacaaatcct tgaaaactat atcgaacatc aacgcgaagt catcacacga
     4561 cggacggtct ttgaaaaaga aaaagcagaa gcaagagcac acatcttaga aggtttgcgt
     4621 attgctttgg atcatatcga tgaaatcatt gcgattatcc gcgggtctaa atcagatgac
     4681 gaggcaaaag caacaatgat cgaacggttc gatttatccg atcgccagtc tcaagctatt
     4741 ttagatatgc gtttacgccg attgaccggt ttggaacggg aaaaaatcga aaatgaatac
     4801 caagaactct taaaactgat tgaagattta actgacattc tggcaagacc agaacgtgtt
     4861 acagaaatca tcaagaatga attagcagac ttgaatcaac gatttggtga taaacgacgc
     4921 acagaattac tagtcggtga ggtcttaagt ctggaagatg aagatttaat cgaagaagaa
     4981 gaaatcgtta ttacattgac aaacaatggt tacatcaaac gtttggcaaa taatgaattc
     5041 agagctcaac gaaggggtgg acgtggtgtc caaggaatgg gtatccacaa tgacgacttc
     5101 gtcaaaaatc tggtttcttg ttctacccat gatacattgt tattctttac gaataacgga
     5161 aaagtgtatc gggcaaaagg atacgaaatt ccagaatacg ggcgtacagc caaaggaatc
     5221 ccaatcatca atttactagg tatcgattcg agtgaaagca tccaggcaat cattgcggtg
     5281 acgggagaag cagaagaagg ccactatctc ttctttacga cgcgtcaagg tacagtaaaa
     5341 cgtacttctg tcactgcttt tgctaacatc cgaagtaatg gtctgatcgc aattggtttg
     5401 aaagaagacg atgagctcgt caatgtgcta ctgacagatg gtcaatctaa tatcattatt
     5461 ggaacacata acggctactc tgtcactttc aaagaagaag cagtccgcga tatgggacgt
     5521 acagcttccg gggtcagagg gatacgcctg cgtgaggaag attatgtcgt tggggcagcc
     5581 ttaatgaagg aaaatcaaga agtattagtg atcacagaaa aaggttacgg aaaacggaca
     5641 aaagtatcgg aatatccagt gaaaggccgc ggaggtaaag ggatcaaaac ggcgaacatc
     5701 actgaaaaaa atggtccact agctggattg accactgttt ctggagatga agacatcatg
     5761 ctgattacag ataaaggtgt cattatccgt ttcaacgtat cgactgtttc tcaaactgga
     5821 cgttcaacat taggcgtacg tttgatgaag atggaagctg atacgaaagt tgtcacgatg
     5881 gcagcagtcg aaccagaagc tgcagaacaa acagttgaag aacaaggatc agaagaataa
     5941 ccatcaagac taagtgtgta agctagcaag aataaccgat taaaaaatct ggttattcct
     6001 gctggctttt ttgattagtt gaatttctct taaaatattc gaaactgttc attctgtatt
     6061 gcttttgacc aaaatagagt gtataattag agcttgtgag tagtctaatt aattagataa
     6121 ctctccttgc tcttaggaac ctaagagccg aaagtccaca gggaggtgaa tatcaatgga
     6181 aaacacgaaa tatgaaatca tgtacattat tcgtccaaac attgatgaag aagcaaaaac
     6241 tgctttaatc gaacgtttcg atgcaatctt gaaagataac ggagcagaag ttatcgaatc
     6301 aaaagattgg gaaaaacgcc gtttcgctta tgaaatgaac ggttaccgtg aaggtatcta
     6361 tcacatcgtt aaagtatctt ctccatcatc agcaaatgct gtcaatgaat ttgaccgtct
     6421 tgctaaaatc aacgacgata tcattcgtca catgattgtt aaagaagaag cttaattgtt
     6481 gtttcatgtg aaacaataga aaacgatgag aggagatgac tattttgatt aataatgttg
     6541 tattagttgg acgtctgacg aaagatccag atttgcgtta cacagcaagt ggtacggcag
     6601 tcgcaacatt tactttggct gtgaatcgta attttacaaa ccaaagcggc aatcgcgagg
     6661 ctgattttat caactgtgtg atttggcgca aatcagcaga aactttagca aactatgctc
     6721 gtaaaggaac acttttaggt gttactggac gtatccaaac tcgttcttat gataatcagc
     6781 aaggacaacg tgtatacgtg acagaagtag tagctgacaa cttccaattg ctggaaagtc
     6841 gttctgcatc tgaaaatcgc caacaaagcg gcggattcca aagttctggt caatcagctg
     6901 gcggatttgg cggaaacaat aactctaatc aaacatctca atcatccaac ggtatgcctg
     6961 atttcgaccg tgatacttct gacccattcg gctcaagttc aacgatcgac atttcagacg
     7021 atgatttacc attctaaacg aatttgatag gagggaaata gaatggcaca acaaagaaga
     7081 ggcggacgta aacgtcgtaa agtagactac attgctgcta accatattga atatatcgat
     7141 tataaagata tcgaattact aaaaagattt atttcagaac gcggtaaaat cttgccacgt
     7201 cgtgtaactg gtacaggcgc taaaaaccaa cgtaaaatta ctgttgcaat caaacgcgct
     7261 cgtatcatgg gcttgctacc tttcgttagc gaagactaat aaatcctagg aattgaatga
     7321 ctgatccttt tgatcagcta ttcaattcct ttttttctgt aaaaataacc tctattcgtt
     7381 tagtcgctcg aatgacgaaa gttatggtaa aatcaaatta gtcatatatg ggaggaaaat
     7441 tatggaaaat aaaaacaatc atttatcccc gaaacctctc ttccttatcc tgtttatatt
     7501 aatcgaagta ttggtagtct taattttacc atttcgtttt gttagcgctt cggtcattct
     7561 attgatgaat ttatggggtg ttcgctattg gttgaaaatg aaacgttggc ttattttaaa
     7621 taaacgtgaa aaaatccagc aggcttcgac tgtagctgaa accaatctag cttatgtctc
     7681 agaagtcatg cctgtaggag tgattgctta tcagccatca agccaaaaaa tcgaatggct
     7741 caatccatta gcattgaaaa tcaaagatca aagtggatta acagaggaag aattgctaga
     7801 tacttttttt actgcaaaag aaaaaaagaa aaaccaagtc tatctagcag ataaaacatt
     7861 tcagtttgat atggatacag taaagggtgt tgtttatttt aatgatatta cccaaaacca
     7921 gttgttaaaa ctccatcaga cgaacagtca gccggtgatt ggtattttat ctattgataa
     7981 ctatgcagat gccattgata aattagatga caaagagatt tcttatttga actctttcgt
     8041 cactacacta gtttctgact ggatgaatga ataccatgtc tttttcaaaa gaatcaacgc
     8101 agaacggttt ttcttcgtag cacagatgat cgatctgaaa aagatgatgg ataaaaaatt
     8161 tgatttgctg gatcgctttc gagcagaagc tgaaaaacag agctcgccta tcacccttag
     8221 tatggggatt gcttatggct caagaaccct cgatgaaatc ggtggagaag ctcagaataa
     8281 tttagatgtt gctttagtcc gtggaggcga ccaagttgtc gtgaaagagg taaaagaagg
     8341 agcaaaacct gtttattatg gggggaaatc tgctaagaat gtcaaacgca cgcgtgttcg
     8401 ttctcgagcg atgagtaccg ctttgcgtcg gatttttgat gaaacaggcg atgtgtttat
     8461 catggggcac aagtttccgg atatggatgc tttaggctct gcttttggtg tcagttgtct
     8521 ggcacgcttc aaccagaaaa aagcttacat cgttatcaat gagaacgaga tcattccaga
     8581 tgtagaacgg tgtcttgaag aaatccataa gcatcctgaa ttagaaatgc aactaatctc
     8641 tccagaacgt gccatagagc tgaaaaaaga agaggacctc ctcgtgatgg tggattacca
     8701 taaaccttct ctatcgatct ctcagggcct atatgagcaa tttgataaga ttgtgatcat
     8761 tgatcaccat cgtcgtgggg aagagtttcc aagtaaacct ctgctgactt atattgaatc
     8821 ttcagcttct tcagcttcag agctagtttc tgagctgatc caataccaaa gcagcaaaac
     8881 aaatcgctta gatcgttttg aggccacatt attgcttgca gggatggttg tagatacaaa
     8941 tagtttcaat gtccgtacaa ctgcacgaac ttttgacgtt gccagctatt taaggtcttg
     9001 tggagcagac gcatccttag tacaatattt attgagctcg gatttagcga catacttgga
     9061 gatcagcaat ttgattgctg gaagcgacta tgtgacttct gatattgttg tggcggcagg
     9121 gacagacgaa aaagagtata atagtgttat agcagcaaaa acagcagata cattgctttc
     9181 gatgatcaat atcaatgcag ctttcgtcat tacaaagcgg acagacggct tgattgggat
     9241 cagtgccaga agtaccggca cgatcaacgt ccaaatcatc atggaatcct taggtggcgg
     9301 aggacacttc acaaacgcag ctactcaact ggaaaatacg acagttgagg aaacaaaaca
     9361 gaagctattt gctgcaatca aagaaaatat gaatcaaata tatgataagg agtaatccaa
     9421 atgaaagtaa tttttttaca agatgtaaaa ggaaaaggta aaaaagggga catcaaagaa
     9481 gttccagcag gttatgcaca aaattatcta atcaaaaata acttggcaaa agaagcaacg
     9541 aaaagcagta tcagtcagtt agcaggacaa aagaaagcac aagaaaaaca cgaagctgaa
     9601 atcttggaag aagctaaaaa gttaaaagaa ttcttggaaa aagaagatac tgtagtcgaa
     9661 ttgaaggcga aagcagggga agacagccgt ttgtttggtt cgatcccttc aaaacaaatt
     9721 gctgaagggt taaacaaaca atacaaaatc aaattagata aacgaaaaat cgaattagaa
     9781 aatccgatcc gttcactagg ctacacaaac gtacctgtta agcttcatca tgaggtgacg
     9841 gctacgatca aagttcatgt cgtacaagaa taacaaatct tgatagcgat gttcagctgg
     9901 taagtataac cagaaggaaa aaagacaaac cgaaatttat ctgagaaaac agctgcagca
     9961 atgaaagcaa aagctgcaaa tcgatttcag attgatttcg gtttcttttt tgggaacagc
    10021 aaaatagcat aaagaaaacg agtataataa aagagaacta tttttttatt acactttcgg
    10081 tacgaatgat gacttttctc tcattttctt gtaaaatgaa gcaattgaag acaaggagcg
    10141 atacgtatga atgaagtgtg gcaggatcga atcccaccgc aaaatattga agctgaacaa
    10201 gcggttttag gctctgtttt tcttgacgca gaaacgatca ttgatgccat ggagtacata
    10261 gagccaaaag acttttatcg tcgtggccat caaattattt ttgagacaat gatcgagtta
    10321 aatgatcgta atgaagcgat tgacgtggtt actgtcaaag accggctgga acaagccaat
    10381 ttgttagaag atgccggtgg tttaagttat ctctcagact tggcattagc agttccgaca
    10441 gcagcaaata tcgtctacta tgcgaaaatc gttgaagaaa aatcattgct gcgtacattg
    10501 atccagacag caacaggaat cgttacgaaa ggattcgagc aaggagaaga agttcaaaca
    10561 attttagatg atgccgaacg cagtatcttg gaggtttcgg aaaaaagaaa tcgaagcggc
    10621 tttttatcta ttgctgaagt actgaactcc acgattgaaa atatcgatcg actagctcaa
    10681 aacaatgaag aaatcacagg tctaccaact ggctatcaag gtctggataa aatgacagca
    10741 ggtttccagc ccgaagaact aatcattctt gctgcacgtc cagcggtagg taaaacagcg
    10801 tttgcattga atattgctca aaatgtagga acaaaaacag atcgttctgt tgcgatattt
    10861 agtttggaaa tgggggcaga atctcttgta aatcggatgc tttgtgcaga aggttcgatt
    10921 gaggccagcc atttacgtac aggtcagctt tcggaagaag aatggtcgaa tctgatcgtt
    10981 gcgatgggga gtctttctcg agcaaatatt tttatcgatg atacaccagg gatcaaaatc
    11041 tctgaaatcc gtgcgaaatg tcggaaacta gcgcaagaaa aaggcaatct aggcttgata
    11101 ttgatcgatt atttgcagtt gattgaaggt aacggtaaag agaaccgtca acaagaagta
    11161 tcagatatct ctcgccaact gaaaaaactg gcgaaagagc tgaaagtacc tgtcattgct
    11221 ctttcacagt tatctcgtgg agtagaacag cgacaagaca aacgaccagt gctaagtgat
    11281 attcgtgaat ccggatcaat tgaacaagat gcggacatcg tagccttctt gtatcgagat
    11341 gattattatg aaagagaagg cggagatgac gatgatggaa acgagccaaa aaatgacaac
    11401 gtcatcgaag tcatcatcga gaagaaccga agcggtgcac gaggaacagt tgaactactg
    11461 tttatcaaag aatacaataa gttttcgtcg ctttcgccaa gagaagaatt ttaaagaaga
    11521 ggttgtgagc cttgagcaat cccgagagct ctcgtaagaa gatgcaagga gtaaccgcag
    11581 gaacgactag agtacttaat ttaaacagat ctccaaaggg attgtgacaa aagtcttgtc
    11641 acagtccctt tttccgaata aacggtgttc aatcagctga cccttggtat tagctcagca
    11701 aacgtcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
    11761 tgatcttacg acctcttttc cttaaacggt gttctcctgc caactcctcg gtcacaagtc
    11821 acaatatcat caaacatgag aagcagtttt ttgaaattgc tccttgtgac tcagagttaa
    11881 acggcaggct cacaacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaatac
    11941 gcccaatcaa acgaaaaata tgagaagcta tttttgtttg gctgttctta ttacttgatg
    12001 caggacagct ttttcacaac ctctgattca cggtgttcca aaagtagctt cttcgaaaaa
    12061 taagtcgaaa tttcacaaaa atttgagaag caattttcgt acattccttc ttatttcttg
    12121 aagctgacca cttctgttac aacctctttt ttaataacca ttctaaaaag ataaagttcg
    12181 ttttttaagc gtttttataa aataaaaata aaaatcattc gattttattt gaaatatgta
    12241 tctattattg atacaatggt tcaggattaa ataatataga acgaggtgtt cacatgtcat
    12301 ctgtagtagt agtaggtacg caatggggcg atgaagggaa aggaaaaatc acagactttc
    12361 tcagtgaaaa tgctgaagtg attgcacggt accaaggtgg cgacaatgca gggcacacaa
    12421 ttaagtttaa cggtgtgact tataaactgc atttgattcc ttctgggatt ttttataaag
    12481 acaagatcag tgtgatcgga aacggcgttg tagtcaatcc taaatcattg gttaaagaat
    12541 tagcttattt acatgaaaaa ggtgtagaaa caagtaattt gcgtatttct gaccgcgcgc
    12601 acgtaatctt gccttatcat atcaaattgg accaattaca agaagatgct aagggcgaaa
    12661 ataagattgg tacaacgatt aaaggaatcg gacctgccta tatggataaa gcagctcgtg
    12721 taggtatccg tattgcagac ttattagata aagaaatttt tgaagaacgt cttcgtttga
    12781 atctagatga caaaaaccgt caatttgtaa aaatgtttga ttcagaacca cttgattttg
    12841 acgatatttt tgaagaatat tatgaatacg gacaacaaat caaacaatac gtcacagata
    12901 cttccgttat cttgaatgat gctttggatg caggcaaacg cgtgttgttt gaaggcgcac
    12961 aaggtgtaat gctggatatc gaccagggaa cttatccttt tgttacttct tctaacccag
    13021 tagccggtgg ggtaacaatc ggaagcggtg tagggccttc aaaaatcaat aaagttgtcg
    13081 gcgtctgcaa agcctatacg tcacgtgtcg gtgatggacc atttccaact gaattatttg
    13141 atgaaacagg agaacaaatc cgcgaagtag gacgagaata cggtacgaca actggtcgtc
    13201 cgcgtcgtgt aggctggttt gattctgtag ttatgcgcca ttctaaacga gtttcaggta
    13261 ttaccaacct atctttaaac tcgatcgatg tattaagtgg attggaaaca gtaaaaattt
    13321 gtacggctta tgaattagat ggcgaactaa tctaccacta tccagcaagc ttgaaagagt
    13381 tgaaccgctg caaaccaatc tatgaagaat tgccaggctg gtcagaagac attacaggat
    13441 gcaaaacact tgcagaactg ccagaaaacg cacgtaacta tgtacgtcgt atctcagagc
    13501 ttgttggtgt acgtatctca acattttctg tcggacctga tagaacacaa acaaacatct
    13561 tagaaagcgt ctgggcgcaa atctaaggat aaaaacgacg actgaatcag gacgttctta
    13621 ccagagcgag tcgaagtgtt tatctatacg ataataataa cttgaagagg tagtgaaaga
    13681 gacgtttagc cttattgccg ataggctgtc tcttgaacac tgtttattca gaataaggga
    13741 ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DAFGZE010000076        13640 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.078, whole genome shotgun sequence.
ACCESSION   DAFGZE010000076 DAFGZE010000000
VERSION     DAFGZE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.078"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            155..469
                     /locus_tag="LTU54_002357"
                     /pseudo
     CDS             155..469
                     /locus_tag="LTU54_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(774..1334)
                     /locus_tag="LTU54_002358"
     CDS             complement(774..1334)
                     /locus_tag="LTU54_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBL8297050.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1476..2090
                     /locus_tag="LTU54_002359"
     CDS             1476..2090
                     /locus_tag="LTU54_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8297051.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            2279..4159
                     /locus_tag="LTU54_002360"
     CDS             2279..4159
                     /locus_tag="LTU54_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HBL8297052.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            4238..4465
                     /locus_tag="LTU54_002361"
     CDS             4238..4465
                     /locus_tag="LTU54_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBL8297053.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4470..5012
                     /locus_tag="LTU54_002362"
     CDS             4470..5012
                     /locus_tag="LTU54_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBL8297054.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            5239..5955
                     /locus_tag="LTU54_002363"
     CDS             5239..5955
                     /locus_tag="LTU54_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL8297055.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(6015..8033)
                     /locus_tag="LTU54_002364"
     CDS             complement(6015..8033)
                     /locus_tag="LTU54_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBL8297056.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8046..9044)
                     /locus_tag="LTU54_002365"
     CDS             complement(8046..9044)
                     /locus_tag="LTU54_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297057.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            9242..10483
                     /gene="sstT"
                     /locus_tag="LTU54_002366"
     CDS             9242..10483
                     /gene="sstT"
                     /locus_tag="LTU54_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBL8297058.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10630..11265
                     /locus_tag="LTU54_002367"
     CDS             10630..11265
                     /locus_tag="LTU54_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HBL8297059.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11456..12196
                     /gene="truA"
                     /locus_tag="LTU54_002368"
     CDS             11456..12196
                     /gene="truA"
                     /locus_tag="LTU54_002368"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBL8297060.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(12341..>13640)
                     /locus_tag="LTU54_002369"
     CDS             complement(12341..>13640)
                     /locus_tag="LTU54_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297061.1"
                     /translation="MKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLP
                     LILMGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIA
                     RLLSLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYN
                     NYTIGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQ
                     FVWRLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKD
                     SLTLIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLT
                     IELDNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQ
                     YTKLAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4563 a         2156 c         2669 g         4252 t
ORIGIN      
        1 atagagaatt gggctatttt taatggcaaa gaggggaaga agatgtttca aaacaaagga
       61 caacgctatg ttgtaaaagg agtaaaggaa tcttactgga atcaaaaatc tttattgaga
      121 aaattatctt tgaatttaag cacaaagaaa tgaaggtaca ctagatagca atgaagatat
      181 ctttgtcatt aaacgtcgct attttacaca agtaagtgat gatttgattc atgtcttttc
      241 caatgagtgg tttgtttctg agaaaaaatt acatgcttct aatctgcaat atatgccagg
      301 tgaagatcca attcctaata tgaaagcaat tatcaacagt aaggattacg aaggatataa
      361 agccaaacat ccagaagcga aaccatttaa atacccacaa gaaatgaagc gagcatggcg
      421 taaaatgtta gatgatgagc tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat
      481 tcaataaact ggatgataaa aatataatat gactataaat tggttacagc ttcaacctat
      541 aagttgtaat aaattagcct aaaagtaatt tattaatgat gaggtgaatt tgattggtac
      601 aacaagatat atcacttaga gagggagaag cagcactaca aactactttt caataagtag
      661 gaattataga aattttactc aaaaaatctt cagtaaacaa tttggctaaa taagattcag
      721 ttgttatacg agaaggttac ggttcttctt aactaagctt agtatgtttt tattcaaggc
      781 atgattttct ctatgtgatc attcagtttc tgtaagagtt ctaataattc ttttttggag
      841 tacttgtaat tgctttctac ccaccgattg agaacggata gacaaccttg gacgtggtac
      901 gtagtaaaga atctcatttc ttctgtgata atcgttttct tgtcttcata tagccatatg
      961 ttcagatagt tttcttcaat aacttgttta actttcttct gaaacgaaat atcaccatgg
     1021 ttgaggataa ataagcgata taaaaaagta tttgcgtaaa catagtcaat caagcgagtg
     1081 tatagttcct catagctatg ggctggatca taagagagaa tttcatttag atcccgaaaa
     1141 aagttgcttt ctaattgatc atatagatca tagatatcct tataatgtgt ataaaaagtc
     1201 gaacgatgta catctgccgt ttctgttagt tcgcgtatag tgattgcttg aatcttttta
     1261 tttactaata atgtggatag tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta
     1321 tctgtctttt tcatttacct atctccttat ccgacatatc aaagcaaagc tgtcgcttaa
     1381 tcgacaaact tatagatgat tgattataga tagtagacac tgtgtctatt ataatgtaag
     1441 tataacacaa tcattgaagt tgaggaggcg aaatgatgaa actaactttt aaacccttag
     1501 aaaagaaaga ttacaaaaaa gctatctatt ttgctgttga aggaatgaac tttgagagat
     1561 atatggaaaa tgaacttctg ctgaatcttt acggaagata ttttttgtat atggagttga
     1621 atcgagcaac tcaaattctt gctttgtatg cagaggacga gttggcaggt gttttgctgg
     1681 cggagataaa aggagaaaaa aagaaacatc gttcttttgg acagcaaata tatgttaaga
     1741 tattcgattt tttacaaaat atatttgcta aagaaagtaa aggagcttac gagacagcaa
     1801 atgaacaaat gcttgcagca tatttaaaga agcattcgcc agatggagag atcatttttt
     1861 tagctgcaaa tcctgatttg aaaacaaaag gcatcggtac caagttgcta acagaatttg
     1921 aaaaaagaga gaaaggaaaa gaaatattcc tttttacaga tagtggctgt acgtatcaat
     1981 tctatgaacg acgtggattt gaaagactaa aggaaaaaat tagcaagata aaaatcttag
     2041 ataaagaaat agatctccga tgtatgctgt acaggaaaat cattcaatga aaatttcgat
     2101 agaaacgaca atagttggca agatcacttt tagtgagtct tcttgctaac tattttttgt
     2161 agtgtattct accaaaagaa aaccgccttt ttgactaaac ttgatagtgg tcaatttttt
     2221 atgatggata agtatctata cttagtctcg taagaatgaa aggaaaagag ggagaaagat
     2281 gaaattacaa aagtggatcc aagagcatcg aaatcatatc accgccttat cagctgtgtt
     2341 gattattttt ggttttatcg gaaaatttac tggttttgtc ttgttctata acattgctct
     2401 agccatagct tctattattg cggtcatacc tattatgatc catgcgtatc aggcagtgaa
     2461 agtcaaagtt atctcaattg atttactcgt tactattgct gtgcttggtg cctttatgat
     2521 cggagaatac aaggagtcag ctatcgttac attcttgttt ttgtttggta gctatctgga
     2581 acaaaaaaca ttgaccaaaa cgcgggagtc aatcaaggct ttgaccgaga tggccccatc
     2641 tactgctagt gttgtccaag aagatggaac gactgaagaa gtggaaatcg atgacgtgga
     2701 agaaggcgat atcgtcttag taaaaactgg tggagctatt cctgtggacg gacgagtcat
     2761 tgacgggaaa gggtatgcgg atgaagccgc agtgactggt gaatcacgac aagtcgtcaa
     2821 aatcagtgga gatgaagtct tctctggaac gattttagca gatggctacg tgacgattga
     2881 agcaactaag gtcggagatg atacgacctt tgccaaaatt atcgaattag tagaagaagc
     2941 acaagatacc aaatcccatg cggaaaaatt cattgatcga ttttctcagt attatactcc
     3001 aaccgtttta cttattgcgt tggttgttgg tctgattagt cgtgattttc gtttagcaat
     3061 cactgttttg gttcttggtt gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt
     3121 agcaggaatc ggtaatggag cgaaacgggg catcttagtc aaaggcggtg aagcaatgga
     3181 tactttttcc aaaatcgata cttttgtctt tgataaaact ggtacattga ccaaaggaac
     3241 tactgctgta tccactatca agaactatac agaggatgaa ggggacagct tagctttagt
     3301 ggctcgatta gaaacgttat ccgatcatcc tctggggcgc gccgtgatcc attatgctca
     3361 ggagaaagac attgattttc aacaattagc agtcaaagat aatcagacaa tcaagggaca
     3421 aggaatcaca gctgagattg atggtcatac agtttgtgcg ggcaacacca aattgattgc
     3481 cgctcacaaa ctttctctca caccaaagca agtacaagat ttacatcaat tacaacaaac
     3541 aggcagctcg gtcattattg tagcaatcga caaaaaaatc actcaaatca taggtgtgtc
     3601 agatgtgatt cgaccagaag tcgctgaaca attagctaag ttgaaacaag caggagcaaa
     3661 acagttagtg atgctgacag gtgataatca gatgactgct gattatgtag ctgatatgtt
     3721 aggcattgat gaagtccatg ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa
     3781 ataccaagaa caaggattgc gcgttgcttt tattggagat ggaatcaatg attctccctc
     3841 tttggctgcg gctgatatcg ggattgctat gggatcagga acggatgttg ctatcgaaac
     3901 gagtgacgtt gtcttgatgc aatctaattt tgaatcgttg gttcatgcct accgcttggc
     3961 taagaaaact gttttaaata cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt
     4021 tttactcaac ggcttatttg ccgggtttat ttacatggct agtgggatgt tcgttcacga
     4081 agctagtatc ttgatcgtta tctttaacgc gatgcgttta ttgtattttg gacacgaaag
     4141 caaagaagaa aaatcgtaaa acttgataag aatcaatttt tttatggaag ctgtaatcta
     4201 gaataagact atcaagaaaa aggaaggaag aactacgatg aaaaaaatta ttatgcaatt
     4261 agatacacta gcttgtccaa gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc
     4321 tggggtaagc aatacgaaag ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc
     4381 agatgaagtc caccctgaac aattagctgc agttgtggaa aaattaggtt atgaagtcca
     4441 atcagcaaaa gtaaaagagg cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag
     4501 aaacaaaaca agcagatatc gaccatcata aaccaactgc tggcgccatg gtggggcatg
     4561 tattagcaaa ttttaaatta gaagaaatga aattgatcca agcacaattg tatgttttgg
     4621 gtacatcttc gcaagaatta agtacccttt tttcagaata tgctgatcaa gaagcgagat
     4681 ggttcaataa aatctctcat gagttagtga ttgaaaatga aatcgttccg tctactttag
     4741 aagaagtcat gcgttatgga aaaatttacg aacatggtca aaacaaatat ttaccagcct
     4801 caacaatgtt ggaaaacttt gcaaaagatt ttgatttcca aaatgtcttt atcacacggg
     4861 caatcaaatt agctgaaaaa gaagaaaaat atggcttgca acaattgctt gttgaacttt
     4921 taagtttcaa taaagaaatc atctaccaag tacaaagact ccaaggaaaa actgtccgac
     4981 aagatttgga tgaggaagac gatgatgact aaaagtctat gtttcttcaa atacaaatat
     5041 aaagcgtgag gtaaattgga aaatcacgaa gattaaaata aaagaagcac ccgctgatgt
     5101 attacagaca acatcagcgg gtgcttctca gtttttagaa gatttgaaag attgatgaga
     5161 aaatacgaaa aatcaataaa atcacattgc ttatttgata gaataaagaa gtgggagtaa
     5221 tcgaaaggag ttttttaatt gaaaaaaagg attttagtaa caattggtct agcttttttg
     5281 ttagctggtt gtaccaacgg gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa
     5341 gcaacagttg caactactac aacaactagc acagagagca agtcaactgc agcaagtaca
     5401 gaaacagcag atacctctac ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca
     5461 tctacttcaa gttcttctgt agaggcttca tcttcctctt cagaaagcag cgaacaagtt
     5521 gaacaaacac tgtggaatgc agacaaatcc gcacaattag aggcatttat gaccaaatgg
     5581 ggccaaacaa tgggacaaca atacaaaagt tatacgaatc aaatgagtgt ggacttatat
     5641 ggtcttaaag tccctcaagc tattttgaac ggggagtgga agatggctat tggtggagtg
     5701 cctgtttctg cagaatggtc agaaagtggg acaggtcaag cagactatca aatcacagcc
     5761 gtctattccg atgcagagac agaaccatat ctgaaaaagc acgtctatct attcgggttt
     5821 cagcaaaatc agccaaaagt attggtgaca caacaaaatc aggggaatcc cgataattat
     5881 ctttacttca acgagacagc gaataatgaa ttgaaaaatg gctttaatca aatcgtttat
     5941 ggacaagcaa attaatgaga taaacaaggg gttgtgacat gtcttttatc acaacccctt
     6001 gctcagttta tttgttagtc ttttattttt tgtgtcgccc atgttgcact gtactggtta
     6061 cctgcaacaa gatcatattg tatcagacga taatcttcta gaatttcttt ttgtttctca
     6121 gttaaatttt tttcttggat aatttgacta tcctctgtaa caaagacctg ttttccatta
     6181 aaacgattag acaagctttc tgtaggatca attgttaatg caggtagatg attagatact
     6241 tctgtcaaca acgcatagta aggagttact ttgatattcg catgctcaag agccatagca
     6301 ccaaagttat taggagcaac aacttcttta tttaacgtag ctgcttgttc acgactatac
     6361 ttgttgttat aaatgaaata atctgtctgg tgctgttcta atccatattt tttcgaatca
     6421 ttcccactat agattgcggg taaatggtca ccataaaaaa caaaggtaat cggtttatcg
     6481 attttgtcga tttcttggat gaattttttt actgctgtat cggtatagtg aaggccttgc
     6541 atataagttt gtaattcttt tcttctatta ggaatgacag cagttccttc agcagtgtag
     6601 ttcaattcac tatagtaatc atcataaggc atatggtttt gcatagtgga taattggatg
     6661 aattgtgacg tttccgtatt gctttgtaaa gctttcaagg tttctttgta tgctgaatca
     6721 tccgatatgc gtgggctttg ttctatttta tccgtataag ttaatttatc cggactgtct
     6781 aaataataga acctgtcaaa accaaattta tcaaacactt gttttctgcg ataaagagca
     6841 gctgtaaaag gatggatggc tattttttca tcgaatagat cggtaatatt tggacttatt
     6901 ttctgacgct ccactagctg ggtataaggt gtaactaacg aagcagacag gttactgata
     6961 ctcaagcctg tcagtccttc ccattccatg ttagctgttc cgccaccata accgacggat
     7021 aacatcaatc ctcctgttcc ttctgttata atgcttttga tgtaaggaat agggtctgta
     7081 ggtactgtca gattagggac acgtgcagga tcactgaagc tttcacttaa attgagaacg
     7141 atcgtctgat tgtttaacca gtcacttctt gtttggttga tttcatctgc ctttgtgttg
     7201 tactttttca ttattccatt aattttttct ttcgaatagc cagcaggttc ttccatgatt
     7261 ttaatgtcga tattgtttag gaattgaatg actggtccgt tatcttttac ggcgccttgc
     7321 tggttaaaga aagtcttgtt tactttaaat aaattaaaca gcaagaaaga aggcgaattt
     7381 ttgtgattaa tgaaaaagac acccgaaaaa catcctaata atacagcgat actgataaat
     7441 cgacgtttcc aatgaatctt caaggaatac tgtttttgta gtctacgttg tacaataata
     7501 ctagtaatca ataataggag aaggatcaat ccgcccacga ttaataagac tgggctaacc
     7561 atcgataata gttctgacaa tccggtcagc atttttaaat ctgaaggaaa gacaggctct
     7621 tcccgcattt ccattttgat tactgtcgaa atagtgagca gtaagttaat gatcaaggta
     7681 gcagtcaagg cgtaccaaaa acgattggaa atcaacaata gtaagaagaa aaatcccata
     7741 ataatgatga ttgtcaggat catgggtgct tgtcgctgag taaatatttt taaggcaacc
     7801 tggatagttt ctttccaacc ttcttttggt tctaaaacga aaatctgcac gaaggtaaag
     7861 cagtaaaaaa atgctgcaac taaaaaataa cttttatgaa caaaaagcca atgaattctt
     7921 tttttccaac gctgagttaa ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga
     7981 tgaatgaaac ggattttttg tatataaaat aaaaagataa ctaggaggga cactgctaat
     8041 aaatatcaaa taaaaaactg cccgatattt atcagccatg ccttccctga gtttggaaat
     8101 tcacgtttca taaatgtaga tacgcgataa gaaactaaag ggaaattaca tataacttga
     8161 gtgacaatca aaaatacagc aagaaaattt cctcttcctt ttatacctaa tttatcagat
     8221 agcatttcta aaaataaaaa cgaagagaaa gtataataca ttgcgaagag agaatatggt
     8281 ttagaaaacc tcagcgctgt atctgtattt cctctgatga tgagtgaaat ttgtgtcatc
     8341 aaaataatca aaccgaataa aacaatcaag ctgataaata cttgaggaag cttatagcgg
     8401 accttttctt tccagttcca ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc
     8461 cataaaatac tctgtccatt ctgtaatgca aacgggtctt tattaaatag agtagagcag
     8521 ccaactaatc caattgttaa aaataatagt agatatttaa ttgttgtttt cgataattta
     8581 tttaaaaatc caactgtata aggaatacta caaaaaagca aagtacaaga aacagcataa
     8641 gtaaagtaat tctgactgat aggtgttagg ataagccaat aatcacgaat attcaagctg
     8701 ccagcttgta cgtaaaaaaa gatattgcaa actagtgtgg ctattaggaa tataaaggca
     8761 aaacgactta aataatgtaa aacttttaaa cgggcgattt tttgatattg gtaacccatg
     8821 taaagtggga tcaaatttgc accaataaca acggaagttt gtaaaaatgt atagatgtag
     8881 aatcgaccag ggtttgttgc atatgtagga atagtgactg ctgaaatcaa tatacgctgt
     8941 aaaaccatca ttatacaagc gacaaagatt gtcaggaagc cataactctc cttctttatt
     9001 tttttcaaga ataaacacct cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg
     9061 ataagttgcc cattaataac atcctaactt agaatatctc aaaaaacaac gtgtagacag
     9121 aaaaagtaca aaattagcaa gaaatggttg tttgtattgt tagaattttt agaaaattat
     9181 acctttagtt tctttgcata aaatgataca atgattgtta aaattgtgaa agggtggtca
     9241 tttgttagag aagtttttgg atatgtcgtt agttcaacgt attgttgttg gtattttagt
     9301 cggaatattg tttggtatct ttttccccac atggacattt atttcaatat tgggaacgtt
     9361 gtttgtcagt tgcttgaagg caatcgcacc tattctggtt tttttcttga cgatggcgtc
     9421 aattgccaag cataaaattg ggaataagac attcgtgaag cctattttga tcttatattt
     9481 agtgggaaca ttgctttctg cacttgtagc agttattgta agttcaattt ttactgttcc
     9541 tattacttta caagaaacag tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc
     9601 tactatgttg acgaatgtca cacaaaatcc tgttcaatct cttattgatg cgaattacct
     9661 tggcgtctta ttctgggctg tattgttggg actagctttt cgagcacgtt cagaatcgac
     9721 aaaagagttg gtggatcaga tatctatagc gttgtcacaa gtcgtacaag tgattattgc
     9781 atttgctccg atagggattt taggattggt ttatcaatca attgctacga ctggaatcgc
     9841 tggattggca gagtatctgc agcttttact tgttttgatc ggtacgatgc tgtttgtcgc
     9901 attagttgtg tacccattcc tcacgttttt gtttattaaa gaaaatccct atccattgat
     9961 tttcttttgt ttgaaagaaa gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa
    10021 tatcccgata aacatgatgt tggctgaaag attgaaattg acaaaagagt cttattccat
    10081 atcgatcccg ttaggtgcta cgatcaatat gggcggtgcc gcgattacca tttccttgat
    10141 gacgttgact gctgtacatt ctttagggat tgaagcacct tttgttttga aagtcctttt
    10201 atgtattttg tctgctttag ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt
    10261 attgattcca ttagcatgta gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt
    10321 cggtattgga tttattatcg gtgttatcca ggattcaatg gaaactgcct taaattcgtc
    10381 aagtgatctg ttgtttactg caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa
    10441 aatcaaattg aaagattgtt tagatggatt aagtgaacgt tgaacgttag atatttagta
    10501 tttttaaaat gattcgagtt tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc
    10561 tgttaaatca agataaagtc gtttataata acaattaaaa gcataggtaa ttgttattgg
    10621 aggcgtatca tggatttttt acgtaaattt ggttttttct ttttattatt aggcgtgctg
    10681 agtgattttt tgactccgta cgtattagga ttgtattatc ctgaattaga tcaaatgaag
    10741 agcgtgatca gtgtatttgg agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg
    10801 tctgtggttt cagggatctt tttcatcctg tcattgcctg cattgtatgc tgcatttgtt
    10861 agtacatctc gtgtgttagc taccctagtt acagcagcca tcgcgttcta tggtataggt
    10921 gattgtatct ttacaggttt gttcagtatc aatacccatg aatcttcgtg gaatgtctcg
    10981 acatgggtcc ataatatcgg atcaggttta ggttacagtg gttttttatt gtttccagtt
    11041 cttcttttct tgatctatcg aaaaaatggg caaatggacg taagccactt ataccttgga
    11101 ctgtctgtga tcagtttgct ttttgcggcg atttatggac tggcaagaat tccttctata
    11161 aattcttggc cattactgaa taaaataggt ttttgccaaa gaatcagttt tttctttaat
    11221 tatgtgccca ttgcctggtt gagtatcttg catctcaaac agtaaaactt gagccgtcaa
    11281 attgaacagt cagcggatac ttcatccggt ggctgttttt tgtttttctg aaatatatat
    11341 ttccacagtt ttcttgtata gcttctgtct tagagaagca aggagctata ttttgtgcta
    11401 caatcttaaa aaggtagaga gaagaccaaa tggaagacag aggagccact aaaatatgag
    11461 aaacatcaag ttgacaatcg aatatgacgg aaaaagatat tcaggatggc aaagacttgg
    11521 agatgacgat aaaaccattc aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga
    11581 agagatagaa attattggat caggaagaac agatgctggc acccatgcgc gaggacaagt
    11641 agcaaatttt aaaacaaaca cagaaattgg actggcagaa atgatcgact ttatgaatcg
    11701 ttaccttccg cgggatattg tcatcaaaag aatcgaagaa atgcctgagc gcttccattc
    11761 aagatataat gcggtgggca aaaaatacag ctattatgtt tggaacaacg tgattccttc
    11821 agcatttgag cgaaaccaca gcttttattt ccctcaagag ttggatatgg acaagctgaa
    11881 tgcagcttgc gaaaaattag tgggtacaca tgatttcatt ggtttttctg cattgaaaaa
    11941 atcaaaaaaa tccacagtgc gtacgattga tgaattatct atcgaacgaa agggagaaat
    12001 gcttcatttt acttttgtcg gagacggatt cttatataaa atggtgcgga ttatcatggg
    12061 gacattacta gaaatcggca caggtgccat gccgatagaa acaatcgatg aaatatttga
    12121 aagcaaagtg cggcaacatg caggagaaac cgtccctgca caaggtctgt ttttggatga
    12181 agtttattat cgataaaaat actttgataa tggatgaaac gcatgtttca cagtataaat
    12241 atcgatgtta aataatgtat gaagcaaatt aaagaaatat aaaaaaagac tttttagcct
    12301 gtttatgtgt acagctaaaa agtctttctt aaataaaaat ctagtgagaa tgttgtactt
    12361 ctcttctcgg ttgtttttgt ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag
    12421 taacgataga taaatatctt gaagcaattg ctagttttgt gtactgataa gtaataacga
    12481 ctttatttat acctggagga atagttaatt cagttgtgcc aaacttagat aattttgttt
    12541 gtactagttt gccattaata gaagccactt gccctttata acgataaata ggtgtcgtca
    12601 agacagtatt tttattattc gaattatcta attcaattgt aagtttgtta ctcataaaat
    12661 gagttgttgg atttatttcc tggttaccta gtaaatattg atcattatta atcatatcag
    12721 gatgatagat tgactctttg tttgcataat cagtatgatt gtaattttca gctatagcaa
    12781 cagcgtcttc aggtgctata agtgttaatg aatctttgtt agcatggata gaattgctta
    12841 ctcctgagag atgcaatatt agtgcaagaa acgtaaagac cattattttc catgaattat
    12901 ttttagtaga aaaaatataa tagatcacta tactcatcgt ataggctata aataaagaag
    12961 aaaaaccatt taaccgccag acaaattgta gactcttaac tggtgtatgg ttaaaaagtt
    13021 gccaaggaaa taaattggtt gaacaaaaaa gaacaaatac accaagaaag aaaataaata
    13081 aatcatcagc agttaatttt tttataaaaa taaaagcacc aatcaatccg agaaataaga
    13141 ataatccaat agtatagttg ttatagttat tatttaaaat atgtgtcagc atatcactag
    13201 gagccatacc acttaattct tttcccagtg ggactttcaa ttcttgtgct ctagattgtt
    13261 gaataaaagg aattaagaat cccgcactta aaaaaaaggt gaggacggta gcttttaaaa
    13321 gacttaagag tctagcgatt ttttggtccc aaaaataaaa agaaagaaac aacgttcctc
    13381 caataaatac agaaaccata gcgacagaaa gtaaatgagt atacactact agcgtcatgc
    13441 caatagacaa ccagtaccat ttttggtaat ctctaatata aatttcgtaa caccccatta
    13501 gtatcaaagg caaaaatgtt aaagcgacag cttctcccaa tgaagcacga tggaatatgt
    13561 caatggcacg ataggcggaa aatgtataaa taatcgaaaa taacagcgaa atataacgat
    13621 tgttttttat ttgtttcata
//
LOCUS       DAFGZE010000077        13212 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.080, whole genome shotgun sequence.
ACCESSION   DAFGZE010000077 DAFGZE010000000
VERSION     DAFGZE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.080"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            147..1016
                     /locus_tag="LTU54_002370"
     CDS             147..1016
                     /locus_tag="LTU54_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HBL8297062.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1225..2250
                     /locus_tag="LTU54_002371"
     CDS             1225..2250
                     /locus_tag="LTU54_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL8297063.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2289..3239
                     /gene="manA"
                     /locus_tag="LTU54_002372"
     CDS             2289..3239
                     /gene="manA"
                     /locus_tag="LTU54_002372"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBL8297064.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3333..5036
                     /locus_tag="LTU54_002373"
     CDS             3333..5036
                     /locus_tag="LTU54_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8297065.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5038..7071
                     /locus_tag="LTU54_002374"
     CDS             5038..7071
                     /locus_tag="LTU54_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBL8297066.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7082..7522
                     /locus_tag="LTU54_002375"
     CDS             7082..7522
                     /locus_tag="LTU54_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8297067.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7722..8849)
                     /locus_tag="LTU54_002376"
     CDS             complement(7722..8849)
                     /locus_tag="LTU54_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8297068.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8852..9529)
                     /locus_tag="LTU54_002377"
     CDS             complement(8852..9529)
                     /locus_tag="LTU54_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297069.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9793..11559
                     /locus_tag="LTU54_002378"
     CDS             9793..11559
                     /locus_tag="LTU54_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBL8297070.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11574..12011
                     /locus_tag="LTU54_002379"
     CDS             11574..12011
                     /locus_tag="LTU54_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8297071.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12024..13175
                     /locus_tag="LTU54_002380"
     CDS             12024..13175
                     /locus_tag="LTU54_002380"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBL8297072.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4489 a         2293 c         2730 g         3700 t
ORIGIN      
        1 tttataagag tttttactca ttaatttaca aaaaacaagc aaaatgcgaa ttttctcaca
       61 ttttttttaa caaaatcaaa gttattgctt gaaagaagaa gaatccatcg ttaaaataaa
      121 gcttgacgta gaggggagca agaaatatga aagattttca attgggggct gccacctatt
      181 taaaaagtgt agccattcgg gtgaaagacc gagataaaat gattgatttt tacaagaacg
      241 ttgttggctt tgatttgaaa agagaagaaa acgaattagc cattttagga acagaagaag
      301 ctggaagtga aatgttgctg ctggaagaaa gtcctagagc gaatgatcac atgggggtga
      361 tcaaaaagct gaaccgtttt tcattgatta tccctagcac agaagaaatg gcagatatcc
      421 tttgtcgtat ccgtaaaatg aattatccaa tcaaagaagc tttagaagat catggacgta
      481 tggggatttt gctcgtagac ccagaagaga atgaattgga aatttaccat gaagagaata
      541 agaacgacca aagagaaatt ccagaaacaa tggatcaaga agcattatta agaaaatctg
      601 aagaaaaatt cagtaaacta tcaacaggtt cttattttga aaaaatccat ttaaatgtta
      661 ctaacttaac aaaagaacgc caatttctta atcatgtact tggtttgaag gtacataatg
      721 aagaagaggg catccatgta ttgaatgaag gagatttcca tgttggtttg acagaagcta
      781 gcggaggcgc aattgatttg cctacagatg aagtgattgg acttgatttt ctgaaattca
      841 tgatcgatac tgaaagcatg gaaggactga ttgctcactt gcagcaacta tcagttgatt
      901 tcttcttaga caaaaagcac acagtactca caatctatga tgctatagga atcgaatggt
      961 ggttcgtcag tgagccaaaa gaaaagaata aaactgacat tcgatgcgac gcttaacttt
     1021 ttctttctta acgattgtcg catgtctcgt cattttatga acggtcatac tatcgcttat
     1081 tctagagata gaaagagaaa atagtatacc gggggaaact gcgcggtttt tcgcgcagtt
     1141 tttttgtgta aaaaaggttg attcctcata gattaagctt taagcttgga ctgtaagaat
     1201 ctgatagaaa agcaggagaa aaagatgtat ccacacacga aacagcttat tttgaaaaag
     1261 atggaagaag gattttatcc aggtgtagca gtcgccttca taaaagaaaa tcaagtagaa
     1321 acatttacag ccggctttgc ccaagtcgtt cctgaaaagc agctgatgac agaagatctg
     1381 ttgtttgatg cagcctcact gacgaaagtc gttgcaacca cttcattagt tttacgtcta
     1441 ttagaagatg gaaagataga actcgatcaa ccgcttcata aatatcttcc tgaatttgaa
     1501 aatcagacaa tcactttgcg tcatttactg acacatactt ctgatatcaa aacatggata
     1561 ccaaacagag ataagctatc tcaggaagaa ttgatcgccg cttatttgaa gctagaacca
     1621 ggtgagcaac taggaaaaga agtaaaatat acagatgccg gtacgattct tcttgggttg
     1681 atgctagagg aaatttacca agaaactgca gtggaaatct ttcaagaaca agtattggaa
     1741 ccattaggaa tgatgaacag cttatttctt ccttttcctt ccaaaaaaat cgttcccaca
     1801 gaacaactgc cttcgggtga ggttttgcgt ggacaaactc atgatccgaa agcaagagtt
     1861 ttggctgaac atgcaggtaa cgcgggtctc tttattgatt tgaaagacag cattactttt
     1921 gtccaaatgt atttgaacaa gggaaaaatc cgtaatggtc gttttttaga agaagagacg
     1981 attgattctt tactgcaaga tcaaacaccg aaaaaaaatg gctatcgttc tttaggatgg
     2041 gacttaaaaa aggatgtaga agacggtcat gcgattcttt ttcatactgg ttatacaggt
     2101 acgtttttct tgattgatat aaaaacacaa gaagcgttga tatttttgtc aaatcgcgta
     2161 catcctgtcg atcatcgtgc ggagtatctc atatgtcgag atgaaattgt tgaaaacttt
     2221 ttgaaagaga aagcggtatt accgaaataa tgctataata gctatgaaaa atgagaggag
     2281 tttgaattat gcaggaacct atgtttttaa aaccagtttt tcaagaaaaa atttggggag
     2341 gaagtcgttt acgttcagtc tttggttttg atatccctaa cgataaaatc ggagaagatt
     2401 gggcgatcag tgcgcatcct catggtgtca gtgtagtaga aaatggcgaa ttcaaaggga
     2461 aaagattaga tgaactatgg tcagaacaca aggaattgtt tggccatcct tcagaaccag
     2521 tgtttccatt attgatcaag atcttagacg cagaagatga tctatctgta caagtacatc
     2581 cagatgatgc ctatgggatg aaacatgaag gtgaattagg aaaaactgaa tgctggtata
     2641 tcatcgatgc tgaacctggt gcagaaatca tctatggtca tcatgcaaag acaagagaag
     2701 aactagctga gatgatcaaa gatgggcgct gggatgacct tttgaagaaa gtaccagtta
     2761 aaaaaggtga tttcttttat gtaccaagcg gaacgatcca tgctattggt aaaggaatca
     2821 tgattttaga aacacagcaa agttctgaca cgacttaccg cgtgtatgac tacgacagaa
     2881 aagatgcgca aggacaaaca agagagctgc atatccaaca atcgatcgat gtaacaactg
     2941 tccctgcgaa aacaccagaa ttacaaatca aagaagtccg aaaagggaat tcatcgatcg
     3001 tcacttattt agaaactgag tttttcaatg tatatgagtg ggatattaaa ggaatcacat
     3061 ctttcaaaaa acaagcccct tatacattga tgacggtaat cgaaggagca ggagaccttg
     3121 tcgttgacgg aaaaacatat ccattagaaa aaggaacaag ctgtattatt ccaaatggcg
     3181 tttctgagtg ggcggtccaa ggtgaactag caatcattgc gactgaacca ggcaaatagt
     3241 tcccagatag ctgaaagcag cagacggttt ttcgtttgct gctttttcta cataaaatcc
     3301 gtccatcaat aaatagaagt atggtgaaaa agatggaata caaatggaaa gcaaacaaca
     3361 gcagtcgcgt gtctatgaaa caactgagaa aagctggaca tcacttaaat gaaatgatca
     3421 tgccaaatct aagtctgttt atcgcttggg gaatcttgtc attagcaaca aaaggtgtgt
     3481 ctggcgattt gcacaccaca ttagaagagg tatgccattg gatgatccag ctgcttttac
     3541 ccatcttgat cagttattca ggcgggaaat cggtaggggg acaaaaaggg gcagtcgccg
     3601 gtgcaattgc ttctttaggg ttgatcgctc gttccaatgc tccacaaatc gtaggagcga
     3661 tgattatcgg gccgcttgcc ggcatcgtat atgaacaatt tgtttctaga ttccacgtga
     3721 aattcaaggc tggatatgaa atgcttgtca gcaacctact agtaggactg actggtagcg
     3781 ttatttgtat aattggaatc gtttttattg aaccagtttt aaatagtgtt cacttgttgc
     3841 tcgcctccgt tgtgtcttgg ctagtcacaa tgaatctgtt accacttgtc agtctgatct
     3901 tagaacctct aaaagtacta tttttcaata atgcaatcaa tcatggtgta ttgacacctt
     3961 taggaatcga attctcacaa acagtcgggt actcttcctt atttttgatg gaggcaaatc
     4021 ctggacctgg tttagggatc ttactttcta tcctttgttt tgcagaaaaa caggaaaaag
     4081 taaatgcgag tggagcattg atgatccatg cgattggcgg gattcacgaa atctattttc
     4141 catttgtctt gctgcgaccg tatctatttt tagctgtaat ggctggtggg gcaagcggca
     4201 cgttgatatt tcaatggtta gatacgggat tagctgcacc tgtatcgcct gggtcactac
     4261 tcgtgatttt tgccaatgcg ccttatccac aactagctgg ggtaagtata gggatagcaa
     4321 tcagtacatt tgtgaccttc atttgtgcag cgatactatt gaaattacca aataaagaaa
     4381 aagagaacaa aacggtcaaa gaaaggggag aaatcttgga accatctatc attcattccg
     4441 tcatttttgc ttgcgactca ggtatgggat caagtgctat gggagccgcc ctgctgaaaa
     4501 agaaattagc agcagaaaaa atcgatcttt ctgtcggata cagctctatc tatgaattga
     4561 ataaccaagt aaatcagctg gttatcgtgc aacatgaact agcgggtctt gcaagagaaa
     4621 aagtacctca aagtcaaatc tatgtactgg atcacttttt ggaaacagat atcttgctgt
     4681 cacgaataaa agaagcgatc caatttccaa agcgggaaga aaaaaaagag atagcagata
     4741 tcacagaaca aaaaaagtcg cctgctaata ttgtattttt gtataaaaac aacagacgcg
     4801 gctcacaaac tatgggcatg acgattttaa gtcaattagc aaaaaaagcg aaaaaacagt
     4861 ttgttatctc aaaagaatca atagaaaaaa tcaaaatcca agaagaaacg atttatatcg
     4921 cacaaagaga actagctgta actgaacagc tagaagaaaa gatccctcgg ctagttctag
     4981 ttgaacattg ggtagctacg aatgaatacg aaaaactcgt gaagggagaa atataagatg
     5041 tatttatcgc ccagagaaaa acaattatta gcagaatttc tcagtagccc gtcacctgta
     5101 tctatccaaa aaatgatgaa tttattgaag gtcagtaaac ggactattta ccgcgaatta
     5161 gataatcttg aagtctcatt gaaatcggta aatgcttctt tgaaaaaagc agctagaggc
     5221 tcatttgtca ttgaggcatc agaagaagta ttagagcaat taaaagaaaa tacagatggg
     5281 aaaagtaatg acttatccac tgctaaacga caacacgcga ttttagccga gttgttactg
     5341 acgacagagc cgttgagtct tgctcatttc atggaatcat atcgcatcag taataccact
     5401 ttttatgcag atatcaaaca gttggaagaa agtattcgcc aactgccttt ggaaatcatt
     5461 cggaaccagg gatacgagat cgaaggaccg gaaaaatacc gccggcttct gacagcgaat
     5521 gtattagaat tagaaatcaa tgaatatgaa ctttttcata gtatttcttt cgatgcatca
     5581 ctgaattatt tttttcgatt cgttgatccg caacatctat ctcttgcacg caaagtaatc
     5641 ggggaagaat tgatccaaaa aaagaccaat ctaagtgata gaaagcttga acatttggtt
     5701 ttgatgctga cattgacgat ggagcgtgtg actaaaaatc atttactgac aaatgagaca
     5761 tataatagtc tggcaaacaa agaattacta cagacagcta aacgattgtt ttcacggatc
     5821 gcttctgaga caaaacaact ttatccagta aatgagatcg tcttttttgc aggattatta
     5881 aatgacttca cgaactcatt tgatcagaat ttctttgaag agacgttcga tacacagctt
     5941 gcttatttag taaaacaact gattcaagaa gtaagtgaag agacgaatgt ccatttttat
     6001 gaagatgaaa ctttgtacaa aatgctgttg actcatttat ctggtgtgtt ttcaagagcc
     6061 gttttacaag aagagacact gaccaatccg attttagaaa aaatcatggt tcaatacgag
     6121 gaagttgcga atgcaatccg taaagcagta gagcggattt ttcaagaaaa aaaattatcc
     6181 gaagaagaga tcgcttatat ggtccttcat tttgcgaatt cattggaacg aagtccgaaa
     6241 gaaagcaata tcagtattgc aggattttcg ccaagtggat tagcttctac tagcatgttg
     6301 gaactacgat tgaaaaggta ttttcccttc atccaacaaa ttcatttcta tcggattgct
     6361 gatttaaaaa aaatcgattt acaagaagaa tatgatgttg ttatttctac atctatttta
     6421 ccaggatacg gtggaaaata tattttagtc tccccgctac tgctagaaga tgaagtcaag
     6481 cagctaaaag aagctttttc gcatattgag aagaaaaaaa ggcaaaaaat tacgtataac
     6541 agcaataacg agtggatgat agaagatact tatgaggaaa caatgcattt catggatcaa
     6601 atcaatcaat tactcggtcg tttttttatc caagaactgg ataatcctga gacgatttcc
     6661 gaaacgattc gcagcttatt tccttatttt cctgaaggac tagtaacgga tgaagaggtg
     6721 gttcatcgcc ggctgcttaa gcgatatgaa caagcaccta tcggtattcc tcatacaaat
     6781 atggggttat tccatacatc cagctcattg atttctaaac cgatcttttg tattttcaat
     6841 ttgaaacatc cgttgaccat tgaagggatg gatagacagc cagtcgattt gtcccgtatg
     6901 ttagttatgc tggcgccttc gccaatccaa gaaaaagacg ggattctttt aggaaagatc
     6961 agtggagcaa tcatcatgaa tgacttgaac accgagatct tccgctcagg aaataaagaa
     7021 atcgtttatc agttgttagc gaaaatcctg attgaagaaa taaagaaatg aaggtgaaaa
     7081 gatgatacag ctaccagcag aaaaaatatt gctcgatcaa tcctatcaaa attggaaaga
     7141 agcgttgacc gagatcggcc agtggatgac ccaacaaggg ggaatcaaac cggcatatat
     7201 ccaatcaatg atcgaaagac aagaaaaagc gagtgtctat atcgggaatt tcgtagcctt
     7261 gccacatggg actgacaaag atgaagaagt agtagaagaa ggaatctatt tagtccaagt
     7321 gcctgatggt gtggactttg gaacaaaaga ggaaccaaaa atcgcgacac tcttatttgt
     7381 agtggcttta aaaaaagaac atcagttagc ggtgctccaa gatttagcct ttttctgttc
     7441 agacatcgac caagtaatgg cgctcagtga tgcgaaaacc attgaagaag tccaaatgat
     7501 ttttgaacat ggacatatat aaccaatgtt cacagtaaaa aaagctgcct tgcatcaagt
     7561 aataacttca accaagataa aaataagagg agctattttt gttgggaggt tcttattact
     7621 gcggatgcaa gcttttgaaa actatttatt ctagaaaacc gtttatttga aataggggac
     7681 ggtgacaagg tttttgtcac cgcccctatt atttttgtgt tttaatagct cttttttata
     7741 tagatagtgc ccatacctat gaataataaa acgatactcc atataccaag tgtcaagata
     7801 ccattcatga aagtaacctg ttctgaagct agtggcgaga aaatatctcc tcctatgatc
     7861 acattttgaa aatcaacata agaagttggc aagtaatgac tgattgattc tatccatcca
     7921 atcgaagaaa acaagcgata ctctgctaag aaaagacaaa gaagtgtacc taaaagattg
     7981 actagagaat gttcaaataa agcagataga agaaaactga ttgtacttaa aaagagcacc
     8041 cacaacaaca ggaacagaaa gctctggaaa agatactcat acatcggaag gattcgtatc
     8101 tcctgaccaa tgatcgttcc tacaggataa cggaagtctc ccaaaccatt tttgattatt
     8161 actagtatag agacgatcaa aaatggcatc acgagactta gcatagctgc accataataa
     8221 atggtaaaac ggatgctatg tttctttacc atactatccg gtaatagatt gactagagag
     8281 attacctgct ttcttttttc ataagagatg atggaagaaa gaaacaaggc ggttatacat
     8341 aaaatcaaaa aactagaaat cattcctgaa aaaatatttt cgtagtaatt tgctaaaggg
     8401 agatgcgctg gcaagtcaaa aatagaatat ctttggatct gatgttcttt catataacgg
     8461 agcaactcga cacgtttttg ttgttcaatg attggaattc cttgtctttg accagaaatc
     8521 agtcgatcca gtatatcttt ttcataatga taagttgctt cgaccatttg ctggacattc
     8581 cctgtgtttt ttgcatgtaa gattgcttcg atcagtttgt tgcttttctc taaactttcc
     8641 acaaattctt tctcattctc atcttctttc aacgtttcaa tcaaacgttg catctcttca
     8701 tagttatgat tcagttcatc gaattctgta taagcaagtt tatgatcaaa aaaaagtggg
     8761 aatgcaaaga gcacgaccaa caatagagga aataaaaagt ttactttgtt ttttagtaag
     8821 agattgcgtt caaatgtaag taaagacata gttacacctc atataattta cgataaatag
     8881 ctaaaatctc ttccatattt gaggattctg tgacgatttc tccatttttt agaaaacatg
     8941 ctttagaaca aatcgattgg atattcgcga tagcgtggga agaaagaaga atcgtcttcc
     9001 cctctttctg ccattgtctg atgaggctat tcaagatatc aatacttcct gggtctaatc
     9061 cgttcaacgg ttcatcgaat aacatgacag atgaatcact gatcaagtaa gcagcaagca
     9121 agacatgttg tttcattcct agagacattt ttccaatcgg caaagtaagg tactcagtca
     9181 tcttcaatcg ttggaaaaca gtttggcatt ctactttgct ctgccaatag tatttgatca
     9241 aagtcaaata atcaacagcc gatagatagt cggataactg attcgttgat tctagaaaaa
     9301 agaaagaccg gcgataatct gtataagcag taggagagat gttgtgaagg gacaattgat
     9361 cataagttgt tggcagcagt tgggcaatgc cattcagcaa agttgttttg ccaaatccgt
     9421 tcggggcaat caaagcagct acttctcctg attctactaa aagattcaat tgtcgaatca
     9481 cacgtttttt accacgtttc atgttcatgt ttttgatcag taatgtcata gataggcctc
     9541 caatataaaa tttaataaca taatagaatg gaaaacatct aaattaaatg ctaacaaaag
     9601 ttattgttta catattataa aaaaattaaa ataacagttt tatttaaaat tgaagttttc
     9661 tgataccgaa atctttttat tttgctgaca tactcaaacc tttggcacaa agtttcgtgc
     9721 caaagtacga ctatttctct ttttagtaag cggatacaat tagagtatca aaaggagaga
     9781 gggagagaca aaatggaacg gacgattccc aaaaacaaca tttcgctaaa agcgagggta
     9841 caaaaattag gcagtacttt atcaagcatg gtcatgccaa atattggagc actgattgcc
     9901 tggggtgtat taacggctct atttatccca gatggttatt taccaaatga ggctttggct
     9961 accatggtaa gcccaatgct aacttatctg attcctttat tgatcggtta tactggcgga
    10021 aaagtaattg ctggtgaccg cggatcagtt gttggggcaa ttgcaacgat gggtgtgatt
    10081 gtcggaacag atattccaat gatgctaggc gcgatgatca tgggacctct tggtggatat
    10141 gccatcaaga aatttgatca gctattccaa aaacggatca aatccggttt tgaaatgcta
    10201 gtcaataatt tttcagcagg tcttatcggt tttggattag cgttattagg tttttctgcg
    10261 attggtccag ttgtcgatgc attgactcaa gcaatggcaa aaggtgtaga gatcatttta
    10321 tctgcccatt tgattccatt gacaagtatt tttattgaac cagcaaaaat cttatttttg
    10381 aataatgcaa tcaatcatgg gattttgaca ccattgggaa ctgaacaagt agcagaggct
    10441 ggaaaatcta ttctattctt gcttgaagca aatcctggtc caggacttgg cgtattactt
    10501 gcttttactt tgtttggcaa aggagcagca aaatcttcag caccaggagc catgatcatt
    10561 cactttttag gcgggatcca tgaaatctat tttccttacg ttatgatgaa accgttatta
    10621 ttcttatcag ttataggcgg aggaatgtca ggaagtttca ttttccaatt gatggatgct
    10681 ggactaagag cgccagcttc tccagggtcg atcattgcga ttctagcgat gacaccgttc
    10741 aattcttacc ttcctgttat tttgggcgtg attgttgccg cagctgtttc gtttggtatt
    10801 tctgcagtaa ttttgaaagc ggatgcgaaa gatacaaatg aagacttcga aaaatctgtt
    10861 caagagacaa aggcagctaa gcaagcttcc aaaggtgccc aaggtattcc tcaaggagct
    10921 acattctctg gcgtacaaaa aatcattttt gcttgtgatg caggaatggg atcaagtgcg
    10981 atgggcgctt cgatcttgag aaaaaaagta caggaagcag acttgcctca aacggtgacc
    11041 aatcaagcaa tcagccaact gacagatgac tcgaatacat tgatcgttac tcaggtcgaa
    11101 ctacaagaaa gagcaaaaca aaaagcaccg agcgcgcgtt ttgtctcagt agaaaacttt
    11161 ttgaattcac ctcgatatga tgaaatcatt caagaattaa gcagcaagca gaaaacatca
    11221 gtttctgaaa ttgagaatga gccagcagaa gagtttcttt cagcaaacat caaacaaatc
    11281 aaagagattc tacttgtcca cgatgatcgt tccggatcag caacaatggg gatgaccgtg
    11341 ctcaaagata tattagacaa gaaccattta actatcccgt taagaaaagt caatatccaa
    11401 gatttaaaag atgaacctga ttcactgatc gtagccaaag aaagtttagc ggaaaaagta
    11461 cagatggctg cacctcaatc cattcattta tcagtcaaca gcatggtcaa tccgcaaaaa
    11521 tacgaatcga ttgcacaata tttgaaacag acagcttaag ggaggaaaac agcatgcaaa
    11581 gtttgccatt cgacatgatc gagctcaatc gatcttttaa aaccaaagaa gaagcgatcc
    11641 gctattgcgg tcagatgctt gtagaagcag ggtgcgttga tgaggaatat atcgatgcca
    11701 tggtagatag agacaaaatg ttatctgttt atatggggaa ttttatcgcg attcctcatg
    11761 gaacagatga agcaaaaaaa tatgtgaaaa aatccggcat ctgtgtcgtt caagtaccag
    11821 atggtgtaga ttttggtacc gaagaagaag aaaagattgc gactgttttg ttcggtattg
    11881 caggagtcgg agatgagcat ttgcaacttg tacagcaaat cgcactttat tgcagtgata
    11941 tggacaatgt tgtccagcta gcagacgcgc tcagcaaaga agaagtaacg gaaaatctag
    12001 caattgctta aggagagaga aaaatgagag cagtacattt tggtgcagga aatatcggtc
    12061 gaggatttat tggagaggtt ttggcagcta atggatttga gattactttc gtagatgtga
    12121 atcacacgat cattgatgct ttaaatgaaa gaaaagaata cgaaatcgaa ttagcagatg
    12181 aatcaaaaga aaaaatcact gtaaaaaatg tcaaaggaat caacaatcaa acaaatccag
    12241 aaaaagtaat ccaagaaatc gctgaagcag atttagttac taccgccatt ggcccgaata
    12301 tcttaccttt tattgctgag ttgattgcaa aaggtatcca agaaagagaa aaggaaggaa
    12361 gcacaacatc tttagatatc attgcctgtg aaaatatgat tggcggttca gcatttctag
    12421 aaaaagaagt gtataaatat cttccggaaa catcatttac tgacaaatat atcggctttc
    12481 caaatgcagc agtcgatcga atcgtcccat tacaacacca tgaagatcct ttattcgtcc
    12541 aagtggaacc tttcaaagaa tgggtcattg acgatagcca aagaaaaaat aaaaaaatcc
    12601 aattaaaagg ggttttatat gtagatgatc tagaaccata tatcgaacgc aaactattta
    12661 gtgtcaatac tggtcatgcg actgtcgctt atacaggtgc cttgttaggt taccagacca
    12721 ttgatgaagc aatgcaagat gcgttagttg tggcacaatt aaaatcagtt ttgcaggaaa
    12781 caggaagttt gctgattgcc aaatgggggt ttgatgcaga gcagcatcat gcgtatattg
    12841 aaaaaatcat tcatcgcttc caaaacaaaa atatctctga tgcgatcaca cgtgtcgctc
    12901 ggacaccatt acgtaagtta ggttatcaag aaagatttac acgtccagtg agagaactac
    12961 aagaacataa cttgacttgc ccacatttga cggcaacaat ggggattatt tttaattact
    13021 atgatcctga agatgaacaa agccgtcaat tgcatgaaat gaagatccat gaaaacttgg
    13081 aacaattgat tcaagaagtc acaggaatca atgatccgaa aacaatcggg aatatcaagc
    13141 aaaacgtgaa ccgttatgcc aagcaagtcg cttaaaatag gatacgaaaa aggggaatgt
    13201 gacgaaattt tt
//
LOCUS       DAFGZE010000078        13020 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.081, whole genome shotgun sequence.
ACCESSION   DAFGZE010000078 DAFGZE010000000
VERSION     DAFGZE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.081"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            87..2252
                     /gene="acm"
                     /locus_tag="LTU54_002381"
     CDS             87..2252
                     /gene="acm"
                     /locus_tag="LTU54_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HBL8297073.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2524..3009)
                     /locus_tag="LTU54_002382"
     CDS             complement(2524..3009)
                     /locus_tag="LTU54_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBL8297074.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3099..3444
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3489..4130
                     /locus_tag="LTU54_002383"
     CDS             3489..4130
                     /locus_tag="LTU54_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL8297075.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            4149..4784
                     /locus_tag="LTU54_002384"
     CDS             4149..4784
                     /locus_tag="LTU54_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL8297076.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4802..5617
                     /locus_tag="LTU54_002385"
     CDS             4802..5617
                     /locus_tag="LTU54_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBL8297077.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5936..7372
                     /locus_tag="LTU54_002386"
     CDS             5936..7372
                     /locus_tag="LTU54_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8297078.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7561..10008
                     /locus_tag="LTU54_002387"
     CDS             7561..10008
                     /locus_tag="LTU54_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL8297079.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGESNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10238..11305
                     /gene="rlmN"
                     /locus_tag="LTU54_002388"
     CDS             10238..11305
                     /gene="rlmN"
                     /locus_tag="LTU54_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL8297080.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11498..12601)
                     /locus_tag="LTU54_002389"
     CDS             complement(11498..12601)
                     /locus_tag="LTU54_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL8297081.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         4445 a         2175 c         2758 g         3642 t
ORIGIN      
        1 atgagtgggg gaggaattga agaaaggatt tttgagagat gatatagtag gttttatttt
       61 tagttaaaat aggggagata gaaaaaatga aaaagtgtag aaatattatt ttatcaatgt
      121 tatttattat tactaatata acatcactga ttcctgttca tgtctatgcg gatgcaggca
      181 gagatatcag cagtaatgtc acttcgttga ccgtggaccc aacgaatatt acagatggag
      241 gaaacataaa agtaaaattc tcgtttgacg aaaaaaaaca aaatatccaa ccaggtgatt
      301 atctttggat caattggcca agtgaaggaa atattcgcgg tgaaggattc caaaaagaaa
      361 tcccattaat gattgaaaat aaaaatgttg gaactttaac ggtcagaaaa gattctgcgc
      421 aggttgtgtt taatgaaaat attaaaaacc tagactcagt agaaggttgg gggcaatttg
      481 agatacaggc cagaaacgta accgataccg gtgaagaaaa tacgggacca ttcaccgtga
      541 cgagcggtga taaaacagct actgtaaatg tgacaaagcc agcttctggt tcttcttcta
      601 gcgtttttta ttataaaaca ggagacatgc tacctgaaga tactaagcac attcgatggt
      661 ttttgaatat taacaataac ggcacttatg ttgaacaacc agttaaaatc agtgatgaaa
      721 tccaaagtgg acaaagatta gatccaagta cgtttgaaat taatcaaata cacttaggtg
      781 aacagaaggt ttatagagga gaagagggaa tccaacaatt tttacaagat ttccccagcg
      841 ctacatttaa tttttcagta actgataatt acattgaaat aactatacca aaaaactttg
      901 tcaatcttag gaaaataatg gtgagttata agacgatcat agaaaaccca gaacaaataa
      961 attttgagaa tcattcggaa gcttggttta aagaatttaa taaacctgct gttgatggag
     1021 aaagtttcaa tcatacagtt aaaaatattt cagcttctgg tggagtcaac ggtacagtta
     1081 gaggcgaatt gaagattttc aaatatatca atgacacaga gattggtatc ccaaatgtga
     1141 ctttcgaatt aagacgagca gatgaacagc ctattcaagg acagtctagt atcctgttga
     1201 cttcgaatga acagggagaa gctagtatca aaggcctgca agtaggagac tatgttgtaa
     1261 aagaaaaaga agcacccaat tggattgatt ttgatccact aagttcaaat gaactcaaat
     1321 tctccataaa cgaaaatgat acagagggag tcagtttacc tatttataac aagaaaaagg
     1381 ttacgaacat cacagcaact aaaatttgga acggtggaac cactccaaga ccgagtattt
     1441 attttaaatt attcagagca agtacaaaca attgggaacc agttcctgat gcagaaacaa
     1501 aacgattaga caatggaata acttcagtga cttgggaaga tatccaacag tatgatgata
     1561 gtggaaacga atatacgttt aaagtgcaag aagtagatca aaacggaaat gattatgtcc
     1621 cttcaggtta tcaaaaaatt gaaaacgggc tagtcgttac aaatgagaat aaagaagtca
     1681 tttcagtctc aggacaaaaa acatgggaag acaatgagaa ccaagacggc aaacgaccga
     1741 cgacgatcac agtaaatcta ttagccgacg gtcaaccgat ccaacacaaa gaagtaagtg
     1801 aaaaagatga ttggcgctat cgtttcacaa atctgccaaa atacagagac gggcaagaaa
     1861 tcatctatac tgtaacggaa gacaacgtgc ccgaatacag tacgacgatt gacgggtata
     1921 acatcaaaaa cagttataca ccggaaaaaa caaatatatc tataccaggt agccatataa
     1981 caccgtggaa aaattttcca gagaagacag agagagagaa aattagaaat ctattctctc
     2041 aaaaattgca aatatttgct catacaggag aatccagcga acaaagaagc aattatatta
     2101 gtaagagagc tgtacctaaa cagataacaa ataaatcaaa aagcattctt cctaaaacaa
     2161 gtagtaaaaa atctagtttt gctgtaatca ttggtttatt gatagtaact atctgtgctg
     2221 ggatattctt acaaaaatat aaaaaagttt agttagaata taatattttt tgttaaatat
     2281 gactgaaatc aaaagaataa aattccgatt tataatcgaa aaaagaagca tttttatata
     2341 tgcttctttt ttcgtagaat caataaatat ggatttggct aactatggaa gaatactcgt
     2401 attagttttc agcgttcata ctagttgtta cattgtttga ttggccaaaa gaatttctga
     2461 tatattttgt gctaaataaa gaaaaagacg cttgaattgg gaaataagaa aactactcta
     2521 tcattacaaa ttttcataaa ctggcagatg ttctaagtaa acaagtatct tttcaaagtc
     2581 ggaaaaatcc ataatcaacg tagtggtatt gatatttgga tgaaagccta cggtatcttg
     2641 acgatcaata gcagcatcga tcacgacttg gatttttttc tctttgtcgt gcatcaatgc
     2701 taatggagtc actgtacctg ctggaacgcc taggaggtca ggaagctgtt cttcagaaac
     2761 aaaagataag cgcttttctt ttaattgttc agctagttgt tttaaatcgg cgtgcttttc
     2821 atcaggcaag atgacaagat agatttgttt tccttttctt gatttcaata atagattttt
     2881 tacttgcgga ccaggtaggg aaaaagggac atttttgact gacgtaatcg caggatgatc
     2941 gactttttga aaagagatac ccagtttttt tagtagttca taagcttctt gttcggttgc
     3001 acgactcatc gcgcatcacc ctcctttttt tattagtgta tcataaaaaa gagttgacaa
     3061 atgaaaaata atcatgtaaa ttacacgtaa agttaaaaca aatgacatcg aaaagaagag
     3121 tagccgttag gtgtttttag agagtctctg gttggtggaa agaggcagca gttaaccgtg
     3181 aaacacatct tggagtcgat tttctgaagc gtatagtagg aaaatccggg gagcccgtta
     3241 cagctgcggt cgttgctaga tgaccagata aggctggctt ttccgtgagg agtcggtaaa
     3301 aaaaggtgga accacgtgat taacgtcctt tgatcttcgg atcaaaggac tttttgtttt
     3361 catctgaacg gcaacgactg tgtgaggttt tttctgcaag gaaaaaataa acagaggtgg
     3421 aaccacgtat aatacgtcct cttggcattt gccaagagga tttttttatt ttaaaggagg
     3481 aaaagaagat ggagtatttg attgaagtta tgccgcaatt attagtagga gcactcacaa
     3541 cattgaaatt atttttattt accttgattg gctcattacc attagggatt gttttagcat
     3601 taggattgtc tttgcatttt aagccgctac gctacttgct tcaaacatat gtatgggtta
     3661 tgcggggaac accgctgttg ttgcaactga tttttatttt ttatgggttg ccgacgattg
     3721 ggatcgtttt tgatcgtttc gaagcagcac tcattgcctt tatcttaaac tatgcagctt
     3781 attttgcaga gatctttcgt ggagggatac aatcgattcc tcaaggtcaa tatgaagcag
     3841 cgcaagtttt gcaattaagt ccctttcaaa cggtgacgaa aatcgtcatc ccacaagtga
     3901 caaagattgt tttaccttct gtagggaatg aagttatcaa cttggtcaaa gattcttcct
     3961 tgatctatat attaggttta ggtgacgtaa tgcgtgctgg gaaaatcgcg atggaacgcg
     4021 acgcgacttt actgcctttg ctaggcgtgg caatcatcta tcttacattg actggtattc
     4081 taacaatcat aatgaaacaa gttgaaggaa aattaaatta ttaccgctaa ctgataggag
     4141 gaagaaatat gattgaattg aagaaagtga acaaagcatt tggtgaaaaa aaaataatca
     4201 aagatttgaa tctaatcatt ccagatggga aaacattagc tatcgtaggt ccttccggtg
     4261 gcggtaaaac cacgctttta cgtatcttgg ctggcttaga aagttctgac agcggagaat
     4321 tttatttaga cggtgtaccg ttcaacccat tcgagcttaa aatgaaagaa caagttgtag
     4381 gagtcgtatt ccaagacttc cagctatttc cacatctgaa tatttttgaa aacattacct
     4441 tagcaccaaa aatggtattg aaacagacaa aagaagaata caccaaaaaa gcagaagaat
     4501 tagcggcttc attagggatt agtgatttat tgaatatcta tccttatcag ctttctggcg
     4561 gacaaaaaca gcgggtagcg attgccagag cattggctat gaatccacgg gtattggctt
     4621 atgatgaacc aacaagtgct ttagatcctg aattacgcca gcaagtcgag cgtttgatcc
     4681 tatcattgaa agaagatggg gtaacccaaa ttattgtcac ccatgattta gtatttgcag
     4741 aaaatgtcgg tgatcaatta ctaaaagttt ccccaattca ttaagaaagg agaagataga
     4801 gatgaaaaaa accagttttc tattcgcttt gatcgcaggt cttcttcttt ttgcaggctg
     4861 ttcgaacaaa aaaacttcag cagataattg ggcgactatc aacgaagaga aaaaaatcgt
     4921 tataggttta gacgatacat ttgttcctat gggatttaga gataaagacg ggaagctcac
     4981 aggatttgat attgatttag ccaaagcagt ctttgaacaa tatgggatta cggtagactt
     5041 tcagcccatt gactggtcaa tgaaggaatt tgaactgaac aatggaacga tcgatttgat
     5101 atggaacggc tattccaaaa cgccggcaag agaaaagaaa gtgcaattta caaaacctta
     5161 tatggaaaac gatcaagtgc tgatcacacc taaagaatca ggaatcacaa actttggaca
     5221 aatgaataat aagagattag gtgcgcaaaa tgggtcatct ggttatgatg tatttacgaa
     5281 acaaccagaa gtattaaaag agatagtcaa aaaccaagat gctgttttgt atgatagttt
     5341 taatgaagca ttgatcgatc tgaaaagcag acgggtcgat ggtctgctga tggataaagt
     5401 atacgcagac tattatttga aacaaagaaa cgaactagaa gacttcaaca taacaaaagg
     5461 ttcttatgag agtgaggatt ttgcagttgg tgcacgcaag tcagatagta cattagtgaa
     5521 aaaaatcaat gaagccctag atcaactaga gaaaacaggt gaattccaaa aaatatccga
     5581 taaatggttt ggcgaagatg ttacgccgag aaattaacaa ataggggact gtgacaaaag
     5641 acgtgtcaca gtcccttttt ccgaataaac agtgttctcc tgccaactcc ttgtgactcg
     5701 gagctgaatg gcaggctcac aacctcttgg taaacggtgt tcaaaagcca gtttttcggt
     5761 attaagccaa atttatgcaa aaatgtagaa agccattttc acaaaatttt tcttactact
     5821 cgaaactaag cgactttttc acaacctcct ttacataatt ttattttgtt gtggaagggg
     5881 tttcgtgtta ttctaaaaga ggaacgattg aaggttatag gaaaggaaga gatgaatgga
     5941 acacaaagaa cttacctcat ttccaaaaga ttttctgtgg ggctctgctt ctgcagcgta
     6001 tcaagtagaa ggagcttggc aagaagatgg gaaaggagaa tcagtctggg atcgatttgt
     6061 acggatacct ggaaagacat ttaaaggaac gaacggtgat ttggcagtag accactatca
     6121 tcgttataaa gaagatattg ctttaatgaa agaacaaggt ctgaaagctt accgcttctc
     6181 tgtagcttgg acacggattt ttcctaatgg acgaggagaa gtcaatcaag ctgggctcgc
     6241 tttttatgag agattgatcg acgaattgat tgaaaacgat attgaaccta tgttgacttt
     6301 atatcattgg gatcttccac aagcattgca agatgaatat aatggttggg aatcacgtca
     6361 gatcattgaa gatttcactc attatgcaga aacattattt gaggcattcc gcgggaaagt
     6421 ccattactgg attagtttga atgaacaaaa tatcttcact tcattaggtt atttgttagc
     6481 agcacatccg ccaggtgtga cagacccgaa acgaatgtat gaagttaacc atattgctaa
     6541 tttagcaaat gcatctgtca tcaataaatt tcacgagtta gaaatgcctg gaaaaatcgg
     6601 accaagcttt gcgtatacgc caaattatcc aattgacagt aatcctgaaa atgttctggc
     6661 tgctgaaaat gcagaagacc tgatggccaa ttattggatg gatgtctata tgtggggaaa
     6721 atatccgatt gctgccatga ggttcttaga agaaaaagga tgggcaccaa cgattgaagc
     6781 tggcgatgca gaattgctag aatctgctaa accagatttt cttgggatca attactatca
     6841 aacggcgacg aatgcattca atccattaaa cggagttggt gctggaaaaa tgaatacgac
     6901 aggaaaaaaa ggttcatcag aagaaactgg agttcctgga atgtataaaa aagttgagaa
     6961 tccatttgtc gaacgaacaa attgggactg ggagattgac ccagaaggat taaggatcgg
     7021 actgcgtcgt atcacaagcc gttaccgcat gccggtactg attacggaaa atggcttagg
     7081 agagtatgac aagttgacag aagataaaca aatccatgac gactatcgaa tcaattactt
     7141 acaaagccac gtcaaagcca tcaaagaagc aatttcggat ggagcagaag tcctaggcta
     7201 ttgcacctgg tcttatacag atttgcttag ctggctgaac ggttatcaaa agcgatatgg
     7261 atttgtatat gttgatcaag acgaaacaca agaaggatct ttggaacgat ataagaaaga
     7321 cagttattac tggtaccaaa aactgattga agaaaatgga gagaaccttt agttcacata
     7381 cttaaaacaa gaaggtacgg ctggatatgg ccgtgccttt tcttgataaa atcatttaga
     7441 aattaagaaa aactgtaaaa taagctgaaa aatgaaagaa agcgtcagtt ctttcaaaca
     7501 aaactttagt tcccttactt ttttttgtat aatgaaactt gatgaaatga gggataataa
     7561 atggcacaaa agaaacggcc tactaaaaaa aggaaaaaga cgaaaaaaac gaaaaagcaa
     7621 aaacagcagc aggaacaaat gctgacgatt ggtgttgggt ttgccttgat tttatttgct
     7681 gtcttcggct ttttaaaatt aggtttttta ggaatattga ttgcaaacgg ctttcggatc
     7741 attgcaggaa atacttacca aatcctctgt cttttattag ccgtattagg attttggatt
     7801 gtcattaaga atacagagtt ttcaattggt aaaaaccgac gctggtttgg cggcatactc
     7861 ttttatttcg gtatcttatt attgttacat gcccatttat ttggcaaact ccatactggg
     7921 gaatcaaata tcatggggac gacatgggat ttgttagcca gtgatataaa acaaagtcaa
     7981 gtagacaata acgttggtgg tgggatgatt ggagcaatcc tttatcattt tacttacttc
     8041 ttgattgcac agcctggaag ttatttagca gcagtgctat tactggcagg aggagcattc
     8101 ctgttcagta atctggaagg ctatcagctg ttaaatggta tccaatcaat tggagaacga
     8161 gtacaggaat tattggaagg tgatcctgcc aagcaagctc gaaagcaagc ggcaaaggaa
     8221 gagagaatga aacagcgcgc agaagcgaaa gaagcaaggc gcttagctgc aaaagaagct
     8281 gcagaaaaag aagctgcaga atatgagaag aaaaaagcgg tgagccagca aagggaaaat
     8341 actgctgatg aatggcagga accagaaaat acagagccgg agcagctaag ttttgttcca
     8401 atcgacagtt ttcaagaaaa tatccatcct gcgaatctag agaaaccagt accagataca
     8461 ccaaaacaga ccaatacagc agaaggtttt tcagatgagt tgccagagga tgacggtacc
     8521 tcattagaat ttgagattga agcagaacag gaaaatcaag actatgaatt accgacagtt
     8581 gatttgcttg attctatacc aactgtggat caaagtgatg agtataaaaa aattgaaaaa
     8641 aatatcggcg tattagaaca aactttccaa agtttcggtg tcgatgccaa ggtcgtcaaa
     8701 gccagcttag gaccatccgt cacaaaattc gaagtccagc cggctgtagg ggtaaaagta
     8761 agtaaaattg tgaacttaac agatgatatc gcgcttgctt tagcggcgaa agatgtccgg
     8821 atggaagcac cgattccagg taaatcactg attggtattg aagtaccaaa tggaaagatc
     8881 agtatggttt cctttagaga aatcatcgaa gcacaaccaa accacccaga taaattacta
     8941 gaagtacctt taggacgtga tgtttctgga cgggtacaaa cggctgatct ttcgaagatg
     9001 cctcacttac tcgtggctgg ttcgactggt agtgggaagt cagtagcaat taatgggatt
     9061 atcacaagta tattaatgcg tgctaagccc catgaagtaa aactgatgat gatcgatccg
     9121 aaaatggttg aattgaatat gtacaacggc atacctcatc tgttgacgcc ggtcgtgacg
     9181 aaccctagaa aagcagcgca agccttacaa aaagtagtac aagaaatgga agaacgatat
     9241 gaaaaattcg cggcaaccgg agtaagaaat atttctggtt acaatgaatt tgttcaacag
     9301 aaaaacttgg aaaacggaac gaaacatcca accttaccat tcatcgtagt gatcgtagat
     9361 gaattagctg atttgatgat ggtagccagc aatgaagttg aagatgcgat tattcgttta
     9421 gcgcaaatgg ctcgggcagc ggggattcat atgatcctag ctactcaacg tccaagtgtg
     9481 gatgttatca caggaatcat caaagcaaat gtaccttcca gaatggcatt tgcggtatca
     9541 agcggtacag attctcggac gatcattgat tctaatgggg cagaaaagct cttagggcga
     9601 ggagatatgc tattcttgcc aatgggtgag aataaaccga tccgtgtgca aggagcattc
     9661 atttctgacc acgaagtaga acgggtcgtt caatttgtta cagatcagca agaagctcat
     9721 tatgaagaga aaatgatgcc gacagatgaa gttgaaacga caggtgctcc agaacagcca
     9781 caagatgagc tgtttgaaga agcaaaagca cttgtcgttg aaatgcaaac ggcaagtatt
     9841 tcccttctcc aaagaagatt tcggatcggt tacaaccgag cagcacgttt agtagatgaa
     9901 ctggaagctc atggagttgt tggcccgtca gaaggaagca agcctcggaa agtattcatt
     9961 gagcaggaag aaacatcaga aacgttgccg gacgataatg acatgtaaaa cttgtgtgat
    10021 gaagggttga aaaacgaata accaataaaa agacaaggca tgatgatacc aaaagaaaaa
    10081 atggtttttt tccatctttt ctaatacggt atgatgtctt gtctttttag tttttgtgag
    10141 attaaagttc tattcgattt atttgtgaag tattgcatta aggaaatgaa tcatgtatag
    10201 tatgtagcga aactttgaga aaaagaggga aaatactttg gaaaaacagt ccatttatgg
    10261 cttgacgaat gaagaattaa tcaattggtt tatagaaaac ggcgagaaaa aattccgagc
    10321 tgcccaagtt tgggaatggt tgtatcaaaa acgtgtaagt aatttcacag aaatgacgaa
    10381 tctatcgaaa caattgatcg aaaaattatc ggagcatttt atcatcaatc ctctaaaaca
    10441 gatggtcgtc caagaagctt cggatggtac agtaaaatat ttgtttgaac tccctgataa
    10501 aaacatgatt gagactgtac tgatgagaca agaatatggc ttgtcagttt gtgtgacgac
    10561 acaagtagga tgtaatatcg gctgtacgtt ttgtgcaagc gggctgctca aaaagaaccg
    10621 tgatctgact gctggagaaa ttgttgcaca gatcatgatg gtccaacatt attttgatga
    10681 gcgcaatctt ggcgaacgcg tttcacatgt cgttgtaatg gggatcggtg agccatttga
    10741 taattacgac aatgtaatgg actttttgca tatcatcaat gatgccaaag gattggcaat
    10801 tggtgctcgg cacattacag tttccactag tggattagca cacaaaatca aggaattcgc
    10861 taataacggc ttgcaagtca atctggctat ttccttgcac gcgccgaata atgaggtgcg
    10921 aacatcgatc atgcggatca accgaagttt cccaattgag aaattgatgg aagctgtgga
    10981 tgagtattta gagaaaacaa accgtcgaat cacgtttgaa tatatcatgt tgaatcaagt
    11041 aaatgataga ccagagcatg cgcaacaatt agcagatcta ttaaaagata agaaaaaatt
    11101 agcatatgtc aacttgattc catataatcc agtcagtgaa catgatcaat attctcgcag
    11161 tccaaaagct gatgtattga gattttatga tgtattgaag aaaaacggcg taaactgtgt
    11221 gatacgtaaa gaacatggta cagatattga tgcagcttgc ggacaattgc gaagcaagca
    11281 aatgaaaaaa acagttgcca aataatcgta tcctactcgt tttttcaatt accataaaag
    11341 gagcagtttt cttaattaag gaactgttca acttaaataa gagaaaagat tctatagcga
    11401 aagctataaa gctttttttg acaagaaaaa ccctcatcaa gcaaagaaat gtcctcaatt
    11461 tgatgacagc ctgcttgatg ggggttttag caattattta actgattttc ttatatcctt
    11521 gctggctttc ttgtgtagca tgataagttt ctacgtcaag aatggcctgt ttaaattttt
    11581 ctcgggtgat ttcataaggc acatgtttaa tatcatttgt tgccatggct ttatctaaga
    11641 ttgcttctaa atcggtcgtg acattgagat tcatgtcggt taacgatact ggaagttggt
    11701 tctttttcat aaacacatag attttatcaa acaaatcgaa ctgctgatcc atcatcaata
    11761 aggtcagtac accataactg acagatgctc cgtgaaggta tccctccatt ttttctagaa
    11821 cagtcgtacc gtaatagaat gagtgtgcca tagcagaatt atagttattg ttaactaaaa
    11881 cagaaaccaa tccggtagta acaatgatat ccgaaacgac ttgttcaatt gcatatgagg
    11941 gacgatcgtc ggcactatct ttcaatgctt gcgtaccata agtcaagaga cgatcaatgc
    12001 acatttttcc gatttggatt cctaatctat ccgtgtagcc tagatcatcg ccacgagcag
    12061 aaaaagtaga ttcgacttct ttggataaag catcgccaat acctgcccat aagtatttat
    12121 caggtgcttc ggcaataatt gctgtatcaa tgaaggtgtg tacaggtggt tttgaaggaa
    12181 agaagagtcc gtccattacg ccttcttcgt tatagatgac acataccgca gtgacgggcg
    12241 aacagttcga accaatagtg ggaaaggtga agactggttt agctagtttt tcaccaatga
    12301 cttttacagt atcgcaagca cggccaccac cgactgcaaa caggacgtct gctttttgga
    12361 cactttcatg ctggatcaac tgttcaatat ttccataagt tgaattaccc ccgtaccaga
    12421 gactggcgac aacctctatt tcctgtgtca aagctacttt tattttgttt tctgcctttg
    12481 aaagtgcagt cttccctcca atcagcacta cttttgtccc atattctttg acgattccat
    12541 ttgctttttc atacgcatct gttccaatgg tataatttgg taagaaaaca ctttgactca
    12601 tattcatttc cttctttctt cgttgaaacg ggttaaaatt ttaaagagtg atcgatctga
    12661 ctgcggctgt agatggagta gaggtgaacc taagacagga taagtgatga ataaggtggc
    12721 atgaacacct tgaaatcatc aataagtatc ctaaaaacga acaagtacaa aaacattgaa
    12781 aacgacaata atgaattttt gttcctctta gtatttctac cgattccctt tgcttttttg
    12841 taattatcta aaaattctaa aaattaagat tattatatga aaacaggttt tatttgtcaa
    12901 ttcataattg gtttttttta aagcaaaaga taatctatat catgatgaac aaacagagtt
    12961 ttttcctcca attgatggct ttcctgatct ttcctatttt gaataaagtg tttctgtata
//
LOCUS       DAFGZE010000079        12906 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.082, whole genome shotgun sequence.
ACCESSION   DAFGZE010000079 DAFGZE010000000
VERSION     DAFGZE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12906
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.082"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            133..3660
                     /locus_tag="LTU54_002390"
     CDS             133..3660
                     /locus_tag="LTU54_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBL8297082.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="LTU54_002391"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="LTU54_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBL8297083.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="LTU54_002392"
     CDS             7448..7789
                     /locus_tag="LTU54_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297084.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="LTU54_002393"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="LTU54_002393"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBL8297085.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIVRKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="LTU54_002394"
     CDS             9146..11566
                     /locus_tag="LTU54_002394"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBL8297086.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LTU54_002395"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="LTU54_002395"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBL8297087.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
BASE COUNT         4381 a         2242 c         2739 g         3544 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg tgcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaag
//
LOCUS       DAFGZE010000080        12752 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.083, whole genome shotgun sequence.
ACCESSION   DAFGZE010000080 DAFGZE010000000
VERSION     DAFGZE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.083"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            51..788
                     /locus_tag="LTU54_002396"
     CDS             51..788
                     /locus_tag="LTU54_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL8297088.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            802..1617
                     /locus_tag="LTU54_002397"
     CDS             802..1617
                     /locus_tag="LTU54_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBL8297089.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            1630..2277
                     /locus_tag="LTU54_002398"
     CDS             1630..2277
                     /locus_tag="LTU54_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL8297090.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            2293..2949
                     /locus_tag="LTU54_002399"
     CDS             2293..2949
                     /locus_tag="LTU54_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBL8297091.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            3111..3932
                     /gene="racE"
                     /locus_tag="LTU54_002400"
     CDS             3111..3932
                     /gene="racE"
                     /locus_tag="LTU54_002400"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBL8297092.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            3935..5290
                     /gene="rph"
                     /locus_tag="LTU54_002401"
     CDS             3935..5290
                     /gene="rph"
                     /locus_tag="LTU54_002401"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBL8297093.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            5291..5809
                     /locus_tag="LTU54_002402"
     CDS             5291..5809
                     /locus_tag="LTU54_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBL8297094.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            5916..6416
                     /locus_tag="LTU54_002403"
     CDS             5916..6416
                     /locus_tag="LTU54_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBL8297095.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(6696..7616)
                     /locus_tag="LTU54_002404"
     CDS             complement(6696..7616)
                     /locus_tag="LTU54_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBL8297096.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            7781..8794
                     /locus_tag="LTU54_002405"
     CDS             7781..8794
                     /locus_tag="LTU54_002405"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HBL8297097.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(8904..9659)
                     /gene="fabI"
                     /locus_tag="LTU54_002406"
     CDS             complement(8904..9659)
                     /gene="fabI"
                     /locus_tag="LTU54_002406"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBL8297098.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            9879..10319
                     /gene="fabZ"
                     /locus_tag="LTU54_002407"
     CDS             9879..10319
                     /gene="fabZ"
                     /locus_tag="LTU54_002407"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBL8297099.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(10434..11195)
                     /locus_tag="LTU54_002408"
     CDS             complement(10434..11195)
                     /locus_tag="LTU54_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8297100.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            11384..12478
                     /locus_tag="LTU54_002409"
     CDS             11384..12478
                     /locus_tag="LTU54_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL8297101.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(12564..>12752)
                     /locus_tag="LTU54_002410"
     CDS             complement(12564..>12752)
                     /locus_tag="LTU54_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8297102.1"
                     /translation="NSNGILRRNGLPKSMDFRKVNQTFISSVSNQRNHIPRKSLNYRT
                     PIEIFLSYVQEAFYSSLI"
BASE COUNT         4108 a         2208 c         2676 g         3760 t
ORIGIN      
        1 tatacatctt taaaagaaat cttactaaat aataatggga gtgtaattct atggcaatga
       61 ttaaatttga tcacgtggat aaatattatg gcaagttcca cgcattaaaa aatatcaatc
      121 tcgaattcga gaaaggagaa gttgtcgttg tcattggtcc ttctggttca ggaaagagca
      181 cgatgcttcg ctgtatcaat ggattagaga cgatctcttc aggtaagctg ctgattaatg
      241 atactgactt gcatgataaa aagacaaaac tgacagaagt ccgaaaaaac atcggcatgg
      301 ttttccaaca tttcaatttg tacccaaata aaactgttct ggaaaacatc aaattagcac
      361 caatcaaagt gttgaagcaa gaccaggcaa ctgcagtcaa aaacgcggag aaatttttga
      421 aaactgtcaa tatgttagat aagaaagatt cttatccttc catgttatcc gggggacaac
      481 agcaacgggt ggcaatcgcc agaggtttag cgatgaatcc tgagatgctg ctgtttgatg
      541 aacctacttc tgctttggat ccggaaatga ttggtgatgt attagacgtt atgaaaaaat
      601 tggctcatga cggtatgtca atgattgtcg tgacacatga aatgggcttt gccaaagaag
      661 tggcggatcg tgttatcttc atggctgatg gagaagtatt ggaagacagc agaaatgtga
      721 gagacttttt cgaaaatcca aatgaagtcc gagcacagca atttatcagt aaagttatca
      781 atcattgata ggaggaaaaa gatgcgcagg ttaaaaaaaa tacgcctgat tttcctttta
      841 ggcattgctt ttcttgcact agccggatgt aaaggaaatg ggttggcaaa tgaagatatt
      901 ttagagcgaa gcaaatctac caatgaaatc atctggggtg tcaagtatga tactcgatta
      961 tttggaatga tggatattga gagcagaact gtacaaggat ttgatgtaga tattgcaaaa
     1021 gcaatcacta aaaagatcct tggtgacaat ggaaaaacag aatttgttga agtcacttct
     1081 aaaactcgga ttccattatt gaaaaacgga aatatcgatg caattattgc aacgatgacg
     1141 atcacagatg aacgaaaaaa acaggttgac ttttcagatg tctatttcga tgctggccaa
     1201 gctttgttgg taaagaaagg aagccaaatc aaaagtgtag atgatttgaa tgcttctaca
     1261 acggttctag cagtcaaagg ttctacatcg gctgcgaata tccgacagca tgcaccagat
     1321 gcaaaaattt tagaattaga aaactatgca gaagcattta ctgctttaca atctggtcag
     1381 ggagacgcga tgactacgga taatgcgatt ttgttaggga ttgcagatga aaatccagag
     1441 tatgagttag ttggtggaac gtttactaat gagccttacg ggattgcgat caacaaaggg
     1501 caagaaaact tcttgaaagc tgttaaccaa gcgctagagg aaatgcatgc agatggcaca
     1561 tacgataaaa tctatcaaaa atggtttcca aatgaaactg aaggaaaagt agagtaaggg
     1621 ggagaggcaa tgagcgaatt gatacaaact tatggaccaa tctttgtcga cgggttcaag
     1681 atcacattgt tttcgagtgt cttagcactt gtgtttagtt tgattatcgg aacgttaatg
     1741 gcaatcttcc aattatcaaa gaataaagta gtgagcgcct tagcaaaggc atatgtagaa
     1801 ttttttagaa acattccatt attaatcatc gtgatgtttt tttatgttgt cgtaccgatt
     1861 tattggttta atctagatgg attccaagca ggtacgattg gattaacgat ctatacatct
     1921 gcttttattg ctgaaacagt acgttcaggg attcaaaccg taccaaaagg tcaaacagaa
     1981 gcaggactat cttctggttt tacctattca gaaatcatgc gttatattgt tttaccgcaa
     2041 gcattcaaaa tcgttatccc gccattaggc aaccaattta tcaacctcgt aaaaaactca
     2101 tctattttag caatcgtagc tggtttagat ttgatgtatc aaggagactt gatcgctagt
     2161 gaaacattca ataccttcga tacgtatatc atcgttgggt tattttactt agttatcacg
     2221 ctaccgttat cttacttaat ggtttatctt gagaaaaaat gggcggttcg ttcatagaag
     2281 gaggggaaat acatggattt tagcggcgct ttttcatgga tgaatatccg ttttttattg
     2341 gaaggattaa aggttactgt tgaagtttca ttgttttcga tcatttttag tttcttgatc
     2401 ggtggtctga caggtgtgat tcgttttgcg aatatccctt tcttttccaa aatccttggt
     2461 gtagtgattg atattatacg gaatcttcca ttattattga tcattttctt cacttacttt
     2521 gccttacctc aaatcggcat tcagatgaac atcttctggt ccgctgtcgc agcattaacg
     2581 atttttgagt cagctatgtt gtcggaaatt ttccgagctg gactcaatgc tgttccaaaa
     2641 ggacagatgg aagcaggatt atcaacggga ctgacttatg tagaatcgat gcgaacgatc
     2701 gtgttaccgc aagcatttaa atcaatgatc ccggcaattg tgagccaatt gatttcactg
     2761 atcaaagata cttcattagc agtaatcatt tctttacctg aactttctca tcaggcacgt
     2821 attatttatg gacagaacac caattatgtg cttccgatgt tcatcatcat gacacttatg
     2881 tacttcgtgg tatgttacgc gctttctctg ctttccggtt acttggaaaa gagacggtac
     2941 agttattaat agatgattct tttagcggtt gtggcaaact aaagtcacaa ccgcttttcc
     3001 tctttccatt cgaaaaaaag agatttcgat tgttttaagc aagtaagagc acttgaaaat
     3061 tcacgctaaa aagtacacaa tagggaggat agaatttatt gaaaaaaagg atgatacgat
     3121 tgacagataa tcgccctatc ggatttattg attcaggtgt cggcggcttg actgtagtaa
     3181 aagaagccct gaaacaatta ccgaatgaaa atattttatt tgtaggagac acagcacgct
     3241 gcccatatgg ccctagaccc gcggaacagg taatccagta tacttgggaa atgacggatt
     3301 atctggtgga gcaaggaatc aagatgctgg tgatcgcctg caataccgca actgcggtgg
     3361 ctttagaaga aatcaaagct gctctttcta ttccagtcat cggtgtgatc cttcccggta
     3421 ctagagcggc agtaaaaaaa acacaaaata aacaagttgg cattatcggt acgattggta
     3481 cggtaaaaag tcaagcttat gaaaaagcac tgaaagagaa agtaccagaa ttgactgtga
     3541 caagtcttgc ttgtcccaaa tttgtttcag ttgtcgaaag taatgaatac cattcatcgg
     3601 tggcgaaaaa aattgtggca gaaacattag ctcctttaac cactaaaaaa atcgatacat
     3661 tgattttggg atgcacccat tatccattat tacgccccat cattcaaaat gtaatgggag
     3721 aaaatgttca actgatcgat tctggagcag aaacagtagg tgaagtatct atgctgttag
     3781 attatttcaa tttgagcaat tcaccgcaaa atggtcggac attatgccag ttttatacaa
     3841 ctggctctgc caaacttttc gaggaaatag ctgaagactg gcttggaatc ggacacttaa
     3901 atgtagaaca tatcgaattg ggaggaaaat aaggatgcgt catgacggga gaaaagccca
     3961 agagcttcgc aaactggagc ttcaaacaaa tgttttcaaa tatccagaag gatcggtagt
     4021 gatccgtttt ggtgatacaa cagttatttg ttctgcaaca atagaaaatt ctgttccccc
     4081 ttttttgaga gaaacaggaa caggatgggt gacagcagaa tatagtatgc tgcctcgagc
     4141 aacgagtacg agaaatcgta gagaaagcag caaagggaaa ctttctggta gaacaatgga
     4201 aatccagcgt cttattggtc gttccttgcg tgcagtagta gacttggaaa aattaggaga
     4261 acgtagtatc atcgtagact gcgatgtgat ccaagcggat ggcggtacta gaacagcaag
     4321 tatcactggg gcatttgtgg cattgaaatt agccattgaa aaacttttaa gagaaaaaga
     4381 attgtcagaa gatccaataa aagaacatct tgctgcagtt agcgtaggaa tcttaccaga
     4441 tgggacttgc gtcacagatt tggattacca agaagattcc gcagcacttg tagacatgaa
     4501 tcttgtaatg acagaatctg gaaaattcgt ggagattcaa ggaactggag aagaagcaac
     4561 gtttgatggt gaacagttga atgaaatgct cttttttgga aaaaacgcga ttgaagattt
     4621 gatcaaagag caaaaacatg ctttgctaac agagtttgct caaaatgatg agcggataga
     4681 agaaacaaaa acaatcatca ttgcgacaag aaatccagga aaagcagaag agtttcgaaa
     4741 tatgttcaaa gaagcgggat atcatgtaaa aacattactt gactatcctg aattgccaga
     4801 tgtggaagaa actggcagca catttgaaga aaatgcccga ttaaaagcag aaacgattgc
     4861 acaattgctt gatcagccag tcttagcaga tgattcaggt ttgaaagtgg atgctttagg
     4921 cggtatgcca ggaatctatt ctgcacgttt tgcaggggaa caaaaaagtg atgcggggaa
     4981 taacgccaaa ttattatatg aattgacaga tgtgccggat gaaaaaagaa cagcacaatt
     5041 ccattgtacg ttagtattcg cggcacccaa aaaagacagc cttgtagtag aagcggaatg
     5101 gcccggtcga gtagcccgta taccatctgg cgaaaatggc tttgggtatg atcctttatt
     5161 tatcccagaa ggtaagaaac aaacagcagc agaattatcg agcgaagaaa aaaacaagat
     5221 cagtcatcgg gctcaagcaa tgaaaaaatt gagtgccgag tggaaacaat ggctggaagg
     5281 agagagatag atgcggtatt tagtagtaag tgataatcat ggagacagag atatcctcgt
     5341 tgacttagta gatcgttatc gttctgaagt ggatatcttt ttccattgtg gtgattcgga
     5401 attagaagct tctgacccat tatgggatac attccaagtg gttcaaggta attgtgatta
     5461 tggaccagga ttcgaacaga aaaaagtgat ccaaactggt caggatacag tattcatgac
     5521 tcacggtcat ttatcgaatg tccgatttgg tttgacacag ttagcaatcg aagctcgagc
     5581 tgcaactgcc gatatggtgt tttttggtca tacccatcaa ataggctgtg agatggatca
     5641 gaaaatcctg tttttgaatc ctggtagcat ttctcagcct agaggcccga tccaaatccc
     5701 atcatatgct cttatcgaaa gtttagatga ccaaatttct gttcaatact ataaccgtgc
     5761 ccatcaggca gttgaagagt tggccttttc tttttcgaga gagcgctgaa taaaaaaact
     5821 ctaattttta ataaaaataa gctttatttt atgaattttg catccggttt cggtaaaata
     5881 gtagtgttga atgaaataga aatggaggcc atcagatgat tggacctata gtaagagaaa
     5941 tgctgctgga aaatcaggaa acgtttttga ttcctgcaga aaatgtggca aatgttatgt
     6001 atcatcatcc attgtcccat ggtttacttg tgttatcgaa ggtcggttat tccaaaattc
     6061 ctgtattagg aaaagatgat cactttgtag gactagtaag tttatcagat gtggtcaata
     6121 aaatgattga cttgcaaaca atcagcatgg acccgttaga aggactaacg gttgctgatg
     6181 tgacagaaac agatgttccg acaattgatg aaaattggga actagaagaa gtactccatt
     6241 tgttagtcga ttcgcctttt ttaccggttg tcacagaaga tcggattttc aaaggaatca
     6301 ttacaagaaa agagctgctt aaagccgtaa acttcatggt gcatgaatta gaacgtcgta
     6361 atatattgtc tccgaaaatc gaagaattaa aagaacaaat cgatatcgtg agctaaagaa
     6421 cgagaaacta aaagataatc aaaaaataag tagaaaaaac agcgtaccag ttttgacttt
     6481 atcttgaaaa agaggctggg acaaaagcgt ttaactccaa gaaataagaa ggaattcacg
     6541 aaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
     6601 ccgccgttta tacgtgttta gagcgtggga caaaagtgtt ctttactttt gtcccacgct
     6661 ctttttagtg agattcgttg catcgtttgt ccaaattatt ttccgacagc aattggactg
     6721 ttcggaatcg tgaaaccaga agctgtcaat tctttgacat aggagctcaa gaactcttct
     6781 tttaaggcat attgttttcc gttcaatgcg tagcagaccg tacgaatcgc aaaattgctg
     6841 tttccaagat ccactaatcc aaagatagaa ggttcagttt ggagctcttc ttggtatttt
     6901 tcagctaatg tacgatttac tcgatcaatg atctcataga ttttgtcata tccttcttca
     6961 ggaacaatcc gaatatctaa aagtacttgc atatttgcac gagagagatt gctgatcgtg
     7021 gtgatgtttc gattcgggat gaaatggaca gtaccatccg ttgctttcag ctgaagggta
     7081 cggataccaa cagatacgac ggttccttca atcgttaagt ttgctagctt gatgtagtcc
     7141 ccaacatcca tctgttgttc catgataata aaaaatccgg taataacatc actcatgaac
     7201 ccttgggcac caagaccgat tgccacacct gcaattccgg caccagccaa aagtgatccg
     7261 atcggcacac cgatagcaga taaaattgca tagataaaaa agaaaccaag ggtataatga
     7321 aaaatagtat ttaaaagact atgtaaagtc tttaagcggc tctcgtttag aatggctttt
     7381 ttagaatact gattaaaaga cttatcaatc agatattttc ccacacggtg aagaatccaa
     7441 aaaaggacga tcaaaaaaat cagatataaa cttttttgga taaaggcagc caaaatggcg
     7501 tcccaatcaa tcccgtccca aaaccgctga aacgcattta ctttgctgac agcttgatct
     7561 gtcaatgtct cgccagaaga gtctgtcgta ccggcagttg ctaataatga aaacataaaa
     7621 tcctcttttc taaaatttga ttctttctgt attgtaccaa gaagttttca aaaaaggaaa
     7681 tagcaaaaat aactataatg aaggaaaaaa gctgaaaata aaatgacaaa taatatggat
     7741 aatgataaaa tgtggagaaa ataacgaagg tggtaagtat atgcaaatcg attggaacaa
     7801 tctaggtttt tcttatatca aaactccttg gcgttttatt gccaaatgga aagacggaca
     7861 atgggaagaa ggagaattga cagaagataa ttacctgacg attcatgaag gctctccagc
     7921 gcttcattat ggtcagcaat gttttgaagg tctaaaagct tatcgtcgaa aagacggcaa
     7981 ggtcaatctc tttcgacctg aacaaaatag tcgacggatg aatcaaagtg ctagacgatt
     8041 gctgatgcca atggtccctg aaaagatgtt cgttgatgct gtcaagcaag tggtaaaagc
     8101 aaatgaagat tttgtgccgc cctatggaac aggcgctaca ttatacttgc gccctttact
     8161 gattggggtc ggtgaaaaca ttggtgtcca tccagctcca gaatatttat ttgtcgtctt
     8221 ctgtacgcca gtaggagcgt atttcaaagg tggactaaaa ccaacgaatt ttattgtatc
     8281 tgattacgat cgtgctgctc ctaaggggac tggtgcagca aaagtcggcg gaaattacgc
     8341 agcgagttta cttccaggaa cagaagcaaa agaactggat tactcagatt gtgtttatct
     8401 tgatccagcc acacatacga aaatcgaaga agtaggtgca gcaaatttct ttggaattac
     8461 aaaagacgga aaattcatca ctccgcaatc gccctctatc ttaccaagta tcacgaaata
     8521 ttccttgttg cagttagctg aagaacgact aggactgcaa gcaattgaag gagatatttt
     8581 cattgatgat ctagatcaat ttgcagaagc tggtgcatgt ggtacagcgg ctgttatttc
     8641 tccaatcggt gggatttatc acaataacaa tcttcatgta ttttatagtg aaacggaagt
     8701 aggaccagta acaaaacggt tgtatgatga attgacaggt attcaatttg gagacataga
     8761 agcaccagaa ggctggattg aagtcgtgga ataatttcaa agtaatgagt tgaaaaaaac
     8821 gtaaaaagaa aaaatcagga agagacttta aaaagttttt tcctgatttt tatatgtttt
     8881 actttaaaga atgcaacaaa agtttatgaa agatggacac ctttgtcaac gtagataata
     8941 tcaccgatga ttccgctgga aagtgggctg attaagaaag cacaagcatt accgacttct
     9001 tcgatcgtca caccttgctg atcaggcgta cgagattcag ataactggat cagtttttga
     9061 tagtctttaa cacctgtgac agctaatgtt ttgattgccc cagctgagat tccattgacc
     9121 cgaattcctt taggcgagaa ctcagctgcc aaataacgga tagcagcttc aagagaagct
     9181 ttcgcaatac ccatcatgtt ataattcggg attgcgcgtt ctgaacctag ataagtcatg
     9241 ctaacgatcc cgctgtttgg tgcaagaaac ggttgtgcgt attttgatac agcaattaaa
     9301 gagtagctgc tgatatcttg agctaattga tagccgtctc tggaaatatc agacacattt
     9361 ccggacaatt cttctttgtt ggcgtaagca actgcatgga caaggccgtg gatctcccca
     9421 gcatattcac cgatggcatt cattgctttt tcgatgcttt cgtcgctaga aacatcacat
     9481 tctacagtga atgcttcttc gccgaccaat ttgactaatg attttttcat tcgttcattt
     9541 tggtaagtat agataatttc tgccccttgt ttcaacattg cttcagcaca accccaagca
     9601 atgctttttt tgttagcgac gcccataatg acgatttttt ttccttctag aaatgacata
     9661 atgactctcc tttgtttctg ataaaaattt gatagtcgta aaaaaataga gaataatagc
     9721 taaaataact tcttgatttc ttaattatta gtcaaaatgt tttgattgtc aaactatttt
     9781 ttcttaaatt aaaaaaagat tgctaaatat ttaggaatat gatactttga tagtcaaagt
     9841 aatgaaaatg gcaattgcct gaaagaagga gtttaattat gtcttacgta ttatctgcta
     9901 ctgaagtaat ggaattgatt cctaatcgtt atcctatttg ttatattgat tatgtagata
     9961 ctttagaacc aggaaaaaag atcgttgcga caaaaaatgt aacaatcaat gaagattttt
    10021 tccaaggtca ctttcctgac aatccagtga tgccgggtgc gttgattcta gaaacactgg
    10081 cacaagcagg ttcgatactt atcctaaaat cagaagagtt tctaggaaaa agagcttata
    10141 tcggtgggat caataaggcc aaattccgtc aaaaagttgt tccaggagat gtgctgaaat
    10201 gtcattttga aatcaccaaa atgaagggat cagtcggtac agcgttatct gcagcatatg
    10261 tagatgataa aaaagtctgt gagtgcgagt tcacgttcat cgtcaaccaa aatgcttgac
    10321 tataaaacct gatatagcaa aacaaaagga agcttacttg ctttttatgt aaaaagaaga
    10381 cgcctgaatc ctagcatacg ttgtcatcta tgcaggattc agacgttatt tttttatttt
    10441 tgttttgctt cttgcagtat attgatttta tcgattttta cttcttcttt tggtttgtct
    10501 gaactatctg tttcgatatt tgcaatagca tccacgacat ccattccttc aatcacttgg
    10561 ccgaagacag tataatggcc atctagtgaa ggataaccgc cgtttttata agcgtcgatg
    10621 attgcttgag gatagtcgtc ttttaaaaga ccatcatgca tatcatctgt attttgaacg
    10681 ataaagaact ggctgccgtt actgttagga tcttgtgagc gagccataga taacgcacct
    10741 cttatattat aaaggtggtt gttgatctct gtttcaaatc cttctcccca aatgctttcc
    10801 cctccagtgc catcgccttt aggatcgcca ccttggatca tgaaatcttt gatgacgcga
    10861 tggaaagtca gtccgttgta gtaaccgtct tttgcatgtg tcataaagtt ttcaaccgct
    10921 tttggtgcgt gttcgggaaa taatttgatt ttgattgtgc ctttattcgt caccatttcg
    10981 accagatctt cattttcggc gacatctttc gtcaattgtg gtaattccaa tgcgttcaaa
    11041 tcgacgctac tggcagtcgt gctttccgca gtagatgtac tttttgaact ttctgtagac
    11101 gaactagaag aagtcgtgtt gtttgcagaa caaccagcag caaacagcaa tgctgctgct
    11161 aaactaatac ttaaaataac tttatttttc ttcataactg ttcctttcca atttactatt
    11221 ttgttatctt taatgataac aaaatagagg gaaaatacct acttaaatta ttggaattta
    11281 tcatttattt taaaagaaaa agaaaaggtg tagaccattt actttgtgtt tgctataatg
    11341 taagtggata caaaaacgaa aagatgcagg aggaaatggt aatatgggaa aattaggaat
    11401 ctcgatttat ccagaacggt caacatttga aaaggacaaa gcgtacttgg atttagctca
    11461 taagtatggg tataaacggg tttttaccag tttgctgcag atcaatgatg ataaagaaaa
    11521 agttctttca gaatttaaag aagtagttga ttacgcgaat agcctaggca tggaagtaat
    11581 ggttgatatc aatccagcgt tatttgaaca gttggagatt tcttacgatg acttatcttt
    11641 tttccataag atgggtgcct acggggtccg cttagatatc ggattcacag gagcagaaga
    11701 agcaaaaatg acgcgcaatc cttttggtat caaaatcgag atcaatatga gttcgggcac
    11761 gaattacgtt gacaacatca tgagttattc gccaaacacg gataatttat taggttctca
    11821 taatttttat cctcatcgat actctggttt aggatacgaa cactttgttt tttgttcaga
    11881 aaaattcaga aaatataatt tgaatacgat ggcattcgtc aattcgcaat ctgctgagtt
    11941 tggcccttgg cctacgcaag atggcttgtg cacgctggaa gaccaccgag atcttgaaat
    12001 tgcgacacaa gtgaagcatt taatccttac aggattgatt gatgatattt ctattggaaa
    12061 tgcgtacgca agtgaagaag aattgaaaga aatggcagaa gcatttaacg cggattatcc
    12121 aacattgaaa gtagatacgg aagaaggtat tacagaaaac gaacgaatct gcttgtttga
    12181 taaccttcat agttatcgag gggatcgctc agaatatatc cttcgttcaa cgatgactcg
    12241 tgtatactac aaagataaag atttcccgcc tcataatacg cgagatatgc atcatgggga
    12301 tgtactgatt gacaatgaag ggtacggtca atacaaagga gaaacacaaa tcgccttgaa
    12361 ggatatgaaa aacgacggac gtgtcaatgt tgtaggaaga atctccgatg acgaattatt
    12421 cctccttgac tttttgaaac catggtcaag ctttaaatta attgaaaata aaaaatagat
    12481 aattagttat tgcagaaata atccgctaga aatagccagt tatttctgct tttttatttt
    12541 gtttttttat aggttatgat ttgtcaaatt aagctagaat aaaatgcttc ttgtacatag
    12601 ctcaaaaata tttcgattgg tgttctgtaa ttcaatgatt ttcttggaat atgattacgt
    12661 tgattgctga cactggaaat aaatgtctga ttcacttttc taaaatccat tgatttcggc
    12721 agtccattac gacgcagaat cccgttagaa tt
//
LOCUS       DAFGZE010000081        12744 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.084, whole genome shotgun sequence.
ACCESSION   DAFGZE010000081 DAFGZE010000000
VERSION     DAFGZE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.084"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LTU54_002411"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="LTU54_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBL8297103.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="LTU54_002412"
     CDS             complement(322..891)
                     /locus_tag="LTU54_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBL8297104.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="LTU54_002413"
     CDS             1077..1979
                     /locus_tag="LTU54_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8297105.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LTU54_002414"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="LTU54_002414"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBL8297106.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LTU54_002415"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="LTU54_002415"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBL8297107.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="LTU54_002416"
     CDS             complement(5002..8322)
                     /gene="dnaE"
                     /locus_tag="LTU54_002416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBL8297108.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSK
                     SIKGQLIYLLGEDNVVFK"
     gene            8553..8738
                     /locus_tag="LTU54_002417"
     CDS             8553..8738
                     /locus_tag="LTU54_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HBL8297109.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8800..9996)
                     /locus_tag="LTU54_002418"
     CDS             complement(8800..9996)
                     /locus_tag="LTU54_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL8297110.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10166..11602)
                     /locus_tag="LTU54_002419"
     CDS             complement(10166..11602)
                     /locus_tag="LTU54_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBL8297111.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11895..12704
                     /locus_tag="LTU54_002420"
     CDS             11895..12704
                     /locus_tag="LTU54_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8297112.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3804 a         2675 c         2250 g         4015 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tactcttgct gtcatccacc caataagcat ctgacagaac
     5101 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
     5161 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
     5221 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
     5281 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
     5341 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
     5401 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
     5461 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
     5521 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
     5581 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
     5641 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
     5701 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
     5761 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
     5821 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
     5881 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
     5941 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
     6001 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
     6061 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
     6121 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
     6181 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
     6241 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
     6301 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
     6361 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
     6421 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
     6481 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
     6541 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
     6601 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
     6661 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
     6721 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
     6781 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
     6841 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
     6901 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
     6961 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
     7021 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
     7081 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
     7141 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
     7201 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
     7261 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
     7321 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
     7381 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
     7441 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
     7501 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
     7561 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
     7621 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
     7681 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
     7741 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
     7801 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
     7861 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
     7921 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
     7981 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
     8041 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
     8101 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
     8161 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
     8221 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
     8281 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
     8341 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
     8401 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
     8461 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
     8521 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
     8581 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
     8641 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
     8701 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
     8761 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
     8821 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
     8881 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
     8941 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
     9001 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
     9061 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
     9121 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
     9181 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
     9241 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
     9301 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa
     9361 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
     9421 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
     9481 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
     9541 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
     9601 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
     9661 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
     9721 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
     9781 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
     9841 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
     9901 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
     9961 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    10021 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    10081 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    10141 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    10201 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    10261 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    10321 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    10381 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    10441 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    10501 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    10561 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    10621 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    10681 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    10741 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    10801 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    10861 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    10921 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    10981 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    11041 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    11101 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    11161 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    11221 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    11281 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    11341 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    11401 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    11461 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    11521 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    11581 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    11641 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    11701 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    11761 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    11821 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    11881 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    11941 atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca atcaatgccg
    12001 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    12061 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    12121 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    12181 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    12241 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    12301 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    12361 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    12421 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    12481 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    12541 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    12601 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    12661 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    12721 acaaaaaaga ggttgtgaca attt
//
LOCUS       DAFGZE010000082        12672 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.085, whole genome shotgun sequence.
ACCESSION   DAFGZE010000082 DAFGZE010000000
VERSION     DAFGZE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.085"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..1795)
                     /gene="uidA"
                     /locus_tag="LTU54_002421"
     CDS             complement(<1..1795)
                     /gene="uidA"
                     /locus_tag="LTU54_002421"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBL8297113.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNF"
     gene            complement(1818..3584)
                     /locus_tag="LTU54_002422"
     CDS             complement(1818..3584)
                     /locus_tag="LTU54_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HBL8297114.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(3597..4391)
                     /locus_tag="LTU54_002423"
     CDS             complement(3597..4391)
                     /locus_tag="LTU54_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBL8297115.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(4401..5777)
                     /locus_tag="LTU54_002424"
     CDS             complement(4401..5777)
                     /locus_tag="LTU54_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8297116.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(5897..6544)
                     /locus_tag="LTU54_002425"
     CDS             complement(5897..6544)
                     /locus_tag="LTU54_002425"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HBL8297117.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(6628..8055)
                     /gene="uxaC"
                     /locus_tag="LTU54_002426"
     CDS             complement(6628..8055)
                     /gene="uxaC"
                     /locus_tag="LTU54_002426"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBL8297118.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(8129..9208)
                     /gene="uxuA"
                     /locus_tag="LTU54_002427"
     CDS             complement(8129..9208)
                     /gene="uxuA"
                     /locus_tag="LTU54_002427"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL8297119.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(9211..10842)
                     /locus_tag="LTU54_002428"
     CDS             complement(9211..10842)
                     /locus_tag="LTU54_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL8297120.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            11146..11817
                     /locus_tag="LTU54_002429"
     CDS             11146..11817
                     /locus_tag="LTU54_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8297121.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
BASE COUNT         4080 a         2502 c         1638 g         4452 t
ORIGIN      
        1 tgaaattggg tattgatgac catcgtttct gtagatagta agcaatcatt ttaggttttc
       61 tatctctagt aaagattcct ttcttatttc cttgcactct taaaatccct tgacttgttg
      121 caaaatctgc aaaattccac acttgttcac caacaaaatt ttcacatgta tctattactt
      181 cgtgatttgc ttcgtaataa gccacttgat actcttctgt aaacattgtt ggtactgtat
      241 catgaaatcc agctactgta tctgctccat attctgtaaa cattattggc gtatcaggac
      301 atctttccat ccatccatct agctcttttt tcaacagata ttttgcatgt gccagatccc
      361 ctcccgaaac ataccaacca taataacgat ttaaacataa aacatcgaca atatctccta
      421 ccttacaaac atctggcgtt gattttagat aagtaacgat tgttacaggt cttttctgcg
      481 gatcaagttc tttagttaat tcaactaaag gttcaaaata ttctttagca ccttcggaat
      541 ccgaatctgg ttcatttgca atagaccaca ttacaacact tgggtggttc ttgtcgcgtt
      601 ctatcaattc cttgatcacc gttttatgag aattttctgt tcgtatctct ttccacgtat
      661 tatgtttttc cacattatca tctaataaag tggccatgaa atttaaatgt aaccctactg
      721 caggtacttc atcaataact acaatacctt ctcgatctgc taatcgcata atttcttctg
      781 agtaaggata atgtgatgtt ctaaaagaat tagcacctat ccatttcatt aaattaaaat
      841 ctttaatatt tacagcctca ttcaaaccac gtccattaac atacgaatcc tcatgcttac
      901 caaatccttt aaaataaaaa ggtttattat taatcaaaaa tttgccattg gtaacctgaa
      961 cactgcgaat accaaattct tctgtatagg tatcgatcac tttattatca tcaattaaac
     1021 taattttcat actatataaa taagcgtcta gaggttccca aaaaattgga ttcttaagct
     1081 gaatcattcc tttctttcct tcagcttcag ctacaagttg agcctcttcg tcatatatcg
     1141 ctattaagat ttttccggtc cattctgtat cgatattgaa ttctacactt gcatccaaat
     1201 cagttattct tgtattaata gtaatatcac ttatatagtc tttcggagtt gtatataact
     1261 taacaggtct atgaatacct gcataattga agaaatcaaa attaactaag ttttcctttt
     1321 ttatatcccc attttcctgt actgtttcct tcataatccc cacaggtaaa gtactatcgt
     1381 caataatatt gtttacagca actgtaattc gattattccc gtcttttaca aaccgattaa
     1441 tttcagactc aaatggtgta aacccacctt tgtgttgagt aataaacttt ccattcaaaa
     1501 aaacttttgc ttcatgtgtt gcagatccaa aacgaagaac taatctttct tctaataatc
     1561 ttttaggaag agtcacttct ttttcatacc acacccatcc aacatgattt ctaatgttct
     1621 ctgtttcaaa taaatcatta taagaagagg gaactgaaat cggataactc tctttttcat
     1681 ttattggttc taaagcaagt tcttcctgta agtcatcatt tgtatctaat ttaaatctcc
     1741 agagaccgct taagtcaatt acttgcctcg tctctgtcat aatgggatac aacatataaa
     1801 ccactcctat ctaaatatta aataaatgta tcctttaaac cacaataagc atctattgga
     1861 cttactccct taaattcaga aagacccatt agtttgtcca ccaatatttc taaagtttct
     1921 tgttttccat cataagtatt aatataagtc tttacttgag gtacatcagc caaatgaaac
     1981 ggacactgta cagatacaaa aattgttgga atttcatgaa tgtaaaacgg tatatccgga
     2041 gttcctttac ttgctggcca gacaattcgt tgaactgtgc ctggattaac attagctata
     2101 ttaatgatca agtcatattt tttagttaaa tttttaattg gtctcttagc agcatatatt
     2161 ccagctatat ttttaatttt ttcttcttgc gataatcctt tcaattcttc ttcaggtgaa
     2221 atgtattttt caacttcaaa tccttttgct gacaaaatct gtttcaataa atcaatggct
     2281 tgaggtctat ttccagcaac tacttcacca aacccacctt ttaccccatg tacatctact
     2341 agtaaaatcc ttttatattt ttctggtaca ataggccaaa tatcttgttt atctttaact
     2401 aatgtaattg cttggtcagc cacctcttta aaaattgatt tattttcaat taaaccaatt
     2461 ttttccagag cttcttgctt aggtaataaa atttgttctt ttgctttttt gtgtaatccc
     2521 aatttagctt ttaaccctaa aattcttttt aaggcatcta ataaccgatc atcagtgata
     2581 attcctttac gatagccttc catcatataa ttaaagtctt catctggatc attaaaaaat
     2641 agaaatagat cacatccagc tgcaatagaa gtgggcaata attctgatcg tttcattgcc
     2701 ccagtcattc ctaacatatg actagcatca gtaactacta atccattaaa tccgagtttt
     2761 cctctaagta agtcagtgat taattctttt gataaagttg caggaagtaa atcttcatct
     2821 ttcagatcag gattaaaatg ttttgaataa gctggcatat ggatatgacc tgccataatt
     2881 gaaggtaatc cagcttctat taagcctgag tatacttttc caaaagaatt atcccattct
     2941 tccgtaccta aactattaat actaaaagat agatgctgat ctcgttcgtc aataccgtca
     3001 ccagggaaat gtttagctgc aggcaaaatt ccactctcct gaataccttt catataagct
     3061 aaactaagtt ctaaaacttt atcagggttt tgtccaaaag atcgactaga aatgatagga
     3121 tttctccaat ttctattaat atctacaata ggagcaaaac tccagttaca tccaatagcc
     3181 gatgcttcaa tcccagaaac tctccctaat tcataagcat atttcgcatc atttgttgca
     3241 ccaattttta cttctacacc aacttcagta ccatctgtac aagctccgtt accacctgct
     3301 tccgtattag cagcaattaa tagaggaatt ttactttctg tttgtaagat atagttttga
     3361 tcatatacct catcagcagt tcctggatta tatcgtacag ctccaatatg gtactgattg
     3421 accatattac tcaagtaatt tttatcacga ctagatccca tattaacaaa caattgacct
     3481 attttttctt ccaaagtcat agaatcaatc gtctcttcaa tccaagaaat agcatcttca
     3541 tctaaattat atggtttttt tcgtaaatct acttttatag tcattttctt tctcctctac
     3601 ttattagtaa gatttttcca aaaagtatct atatattttt gttcaatatc tacataatct
     3661 cccgataaac atttaaataa aacatcagtt cttaagtttt cccatgattc tttttcttta
     3721 ttgaccaaaa taggataaaa gaaaacttta tttttatttg cagcttctag atcaccagga
     3781 gaatcaccaa tcattaatat cttatccaaa tcacaaccat tttcaattac acttcgaata
     3841 atatcttctt tttttccttt atcttgacaa cacagttcat caactaatga taataattca
     3901 tgtctttccc attcttcttg aacagcttcc ttattagctg agcttacaat aattatcttc
     3961 ccatattttt tcagagcttt tatagtatct ctagcactta aaaagggttt atctaatcct
     4021 tttgcttgac taatctgttg attgacttct tccgaccatt ttaaagcttt taataaatcc
     4081 tctgactgag aatattctat ttccctcttc aaagatggat tagacaattc ttttgttaga
     4141 gatacccagt gctttaatcg attaatcgat gttacatcat ttttttctaa tgttttaact
     4201 aaacctaaaa atctgtttat tccacgagtc tctgaatata aattaatatt attccaatcc
     4261 tgtaggaatt tttctctatg atgaacatca aaaatctctg cagcaattgg tccgaaaaag
     4321 agattgtgtt tataagtcat cgtatccata acacagccat ctgaatcaat acaaaatatc
     4381 caattattca tatttctctc ctatttatgt ttttgtttta aaagctcgca attttctaag
     4441 actttatctt tagataatgg ataaaggaaa aataaaatta atcctacaat cagaaaacaa
     4501 attgatggaa ttaatgttga agcgttatat aacttttcta aaacatctcc cgtttgtaca
     4561 tttgctaaag agtcatagcc tataatagaa agagtccaac ctccgacgcc tccagctaga
     4621 gcttgaccta tttttctagc aaaagaatat accgcatata cggtcccatc ttctctttga
     4681 tcagacttta cttcttgatc atcaataaca tcagtaatat ttgcccaaat aacagtatta
     4741 aacacaccca aacccatata gccaaccgaa ctaagaatta agaatacata catattacta
     4801 gcttttatca aaaataagat caaatatact attgctgaaa agaacattcc tactgatgcc
     4861 agttcttttt ttccatattt tttacttaaa aacaaagtta aaggcgaaac aactattaaa
     4921 accaaaatag gattgataaa attcattaaa actattccct gtgcatcatt atagtaatca
     4981 ggaaatacat aattattcat actcataatt aataattgag caaggagtaa gaaaatagcc
     5041 gcaccaatta tagaaagtaa tgccctattt ttaactaacc cttttaaaga ttttaacaat
     5101 gaaactttgt attgagattg gtcaattctt actcgttcag tagttagttt ataacatatt
     5161 aaataacaaa taagtgcaca tatcgaaaat attcccgcaa caattgtaaa agttgatcca
     5221 ccacgaacta tttgagcgcc tgaatcatct cttgtataaa taaataatgg tgttaataca
     5281 ccaatgacca aacttgctaa agtagctcca acatttcgga atgtcgatag cattgttctt
     5341 tcttctgtaa tagaagtaat agctgatgcc attgatccat aaggaatatt tatagatgtg
     5401 taaaaaatac tcccccataa tatatacgta acatacatgt atactacctt aacgctcata
     5461 gaagctgaaa tcatagaagt ttgatacatt aaaaaacttg ctaaagcgac tggaccactc
     5521 attcttaaaa tccacggttt aaacttaccc tgcttagttg ctttcgatct atcaacaata
     5581 cgccccatag ttacatcagt aactgcatcg ataaatcttg ctactaaaaa taatgttcca
     5641 acggcagctc cgcttatccc caatactttt gtataaaaga tcattaaaaa agaactagca
     5701 aaaatgaaag taaaatcgtt tccaaaatct ccaaacatat atcctatttt gtctcttata
     5761 ccaaattttc tttccataca attctctccc ttctgttgtt aagcgttttc attctgatga
     5821 aagtacgtac tatactctga agcgtaaaga gtcagtattt attttttaca tttcagagta
     5881 ttatttgttt tttctcctaa taattcttta tctcataaaa cttatctata tatttactag
     5941 ctactcgact tacttcttca aaatcacctt tttcaacaga agccaataaa ttcccaccta
     6001 caccaactgc aacaactcct acttcaaacc aatcttctaa gttctctata cttactccgc
     6061 cagtaggcat tatatttacg tgtggtaatg gagcttgata ggcttttaca atggatggac
     6121 caaaagcaga acctgggaat agtttaatga tatctacgcc agcagtaagt gcctccttta
     6181 tctcagtaat tgtcatacaa cctggtaaat aaggaatttg atataaatta caaatctcag
     6241 ctgtttgtcg atcaaaagat ggactaacga tatattctgc cccagccata attgcgatac
     6301 gagcagtaaa cgcatctaaa accgtaccgg ctccgattat tacatctttt tgatcagcat
     6361 attttttagc aatttctttg attatttctt cagcattagg aactgtaaaa gttagttcaa
     6421 tccctttaat tccacccgca ataattgaat ttattgcttt aatggcctca tccttatttt
     6481 tcccacgtac aaccgcaatg actccagccg cttctaactt acttaaaatt tctacttttt
     6541 tcatatctat ctccttcctt tatggataca tactttgtct agctgtacta acttacaaag
     6601 aataaattta agcttttatt tcaagtatta ttttctactt ccgatttcaa aaaaattaaa
     6661 ataattatac gcattacgat aacaaatatt ctccactatc tgactaagat acatttcatc
     6721 atctggaatt cggcctgctt taacccactc gcttagtaat tgacataata ctcgacgaaa
     6781 atactcatgg cgaggatagc ttagaaaact tcgagaatca gtcaacatac ctgtaaaatt
     6841 cgacaacaaa ctttgttgtg ccatcgtagt caactgttga cgcattcctt cagctgtatc
     6901 attgaaccac caagcacatc ccaattggat tttttgaatc ataccccctt ggaagctttg
     6961 cattgctgtt gctagtggaa gccaatcagt tggatttaat gaatataaaa ttgtttttgg
     7021 taagcaccca ctcttttcag cggtatcgaa caatgctaca acattttgtg tcaaactagc
     7081 ttgatccccc atcgaatcac caccgacatc tactccaatt tgttccttta atatacttga
     7141 attatttctt aaagcattta aatgcatttg catcgtccaa ccatatcttg tatactcaga
     7201 catcaaatgt aattgaatac ctgtaacaaa ttgtgcaatt tctgataaat ggaaatccgt
     7261 ttttcctgct aaagctttta ttaacagatt gtcgagtatt ttttcatctg ctatttcata
     7321 aaaataagta ttcaaaccgt gatctgctaa tcgacagcca atttgatgaa aatgatccac
     7381 tcgttggctc aatgcttgct tcaacccatt aaagtctttg attggtatag ccgctgtttg
     7441 actaagtttt aaaatatagt tcgaaaagcc tgatgatttg atactccaga ccttatctgg
     7501 acggaaagta ggtaatacct tgaaagaatt atcctgttta gaaagctcag tatgatacca
     7561 caagtcatct gtaggatcat ccgttgtaca gatggctttc acatccatca tctccaacaa
     7621 ttctttcggg cgataggaat ccgtttgaat tttttcgtta gcacgtcgcc aaatttctgg
     7681 tgcattcttc tcagaaattg tcaaatcaat atcaaagtaa cgttgtaatt ccaaatgaga
     7741 ccattcaaaa ataggattac ctggcgcctt ctccactgtt tttacaaaag ctaaaaattt
     7801 ttcataatca tcacctggac cactgatcaa ctcttcttct ataccattag ctcgcatcaa
     7861 tcgccattta taatgatctc cagctccttt atcatttaac catacctgag tcaaattctt
     7921 gtattgctta ttttctagca aatatgctgg gtctaaatga caatgatagt cgataattgg
     7981 ttggtgtttt gcaaatttat gaaaaagtct tttagctatt tcattctcta ataaaaagtt
     8041 cttatctaaa aacatattta aacactcctt tccttccgtt agtttttaga agagaaaatt
     8101 tttcctctaa ataaactccc ttatataatt atttactcgc ccccttttca agcgcttccc
     8161 aaataccatt taaataaact gcacctagag ctcggtcata taatccataa ccaggtctac
     8221 cggtttctcc ccaaatcatc cgaccatgat ctggacgaat atagccatta aatccattat
     8281 cgtacattgt ttttaatatc ttatacatat ctagagaacc atattctgat ttatgcggcg
     8341 cttcgtaaaa atcgagtcca ttgtcactta aatatttgat attacgaaca tgtgcaaaag
     8401 gaatccgatc ccgttgacaa aattcagata gaatttcata gacatcatta tctggtcgtt
     8461 ctgcaatact tccagtacat aatgtaatac cattagaagg agaatccact acattacaca
     8521 accaatctaa atcgtcacga tttttaacaa tacgaggtaa tccgaaaatg gaatatggtg
     8581 gatcatctgg gtgaactgcc attttaatcc caacttcttc acatgtcggg ataattgctt
     8641 tgagaaaata tgcaaagttc tctcgtaatt tctcttcatc aacttcttta taggcttcta
     8701 ataattgctt aatttgtgat aaccgatgag gttcccaacc cggcaattca aaattattag
     8761 tagaattttc tacttctcta ataatttcct ccggatcagt agggatattt tttgaaatat
     8821 aggctaacgt agaagaccca tcatctaaac gataatccaa atccgatttt acccaatcaa
     8881 atactggcat aaaattataa cagataacct caatcccaaa ttctgccaaa ttgcgaatcg
     8941 tctgtttata attctcaatg tattgatctc gtgttggtaa accaattttg atatcatcat
     9001 gaacattaac actctcaata actttcaagg taagtccatt ggcttcgact tcatctttta
     9061 aagatttaat tcgttctctc ggccaaacct ctccggctgg tatatccatt aatgttccaa
     9121 caattccttt cattgctggg atctgacgaa tttcttgtag tgagattttt tcttcatttg
     9181 ccccatacca tctaaaagtc atttccatag ttactttcct ccaacttctg ttatatattt
     9241 attgattgtt ttacgaactg cttgaggacc tactagcatt tcgcaaaaga ttttttcagt
     9301 tttctttcct accccaactt gatataaatc taaaccaaag atttctttat tttctaaaat
     9361 ttcctgtacc gcacaatgaa tctttgaagg atcttgttca cctaatttca atactgataa
     9421 tttatcctgc aactctacca ataacggatc aggacttggc acaaaatttt ccccttgatc
     9481 atcgactgcc agtaaataac gtaaccaagc agcaatagcg ataggaataa atgttagatt
     9541 ttctgcattt ccgtcttttt cgacatactt tttaattgtt tcaccatagc gaatcgctac
     9601 tttttgagaa gtatctgttg caattcgttg tggtgtatct ggaatatagg gattaggcaa
     9661 acgtttatta attacttcat caataaattg ttttggatca ataatgcctg gattttcaac
     9721 tactggtaaa ccttctaaat aacctattcc tttgattaaa ccaaccaaat catcatcctg
     9781 catctctgct gaaattgatt gatatcctag cagacaacct gttactgcca aagcagtatg
     9841 aagtggattc aagcttgtcg taaccttcat gatgtctgct ttatcaaccg tctctctatc
     9901 tgtcataatc acaccagcct tttccaattt aggccggcca tttggaaaac tatcttcaat
     9961 aactaaataa tgaactacct cagtattagt gaaagcagca atattggtat gtttttttgt
    10021 gtgaatgata tctaaatcct cgagccccat catcaataga tcttccttta cattctctga
    10081 aggatttggc gtaattcgat caatcatact ccaaggaaaa gtcacttttt ctttatcctt
    10141 taaataagca ataaattttt cagaaacata tccttctttt tgccaagctt ctgcaatcgt
    10201 aaaaatacta tcttgaaaac gttgaccatt ctgtgagaaa ttatcagtag aaaccatcgc
    10261 aatcggcaac tctccagcat taaatctgct atataacaag gatgttacaa tactcatcgt
    10321 atgttgagga ttgctaggtc cagatttgat atcagctttg actaccgaaa agaattgccc
    10381 tttagaatct tttaaactgt aacctttttc agtaatagta aacgttgtaa actgtaattt
    10441 aggatcttca aaatatttaa ccactctttg atagtccgct atattcttcg gattgcaata
    10501 taaactatca gccgttactg ccaaaagtct ttttttgagt tcaccatctc cactcataac
    10561 aatttccaaa atgtcattgt tatagtcatg ataagctttt tccactactt cttcatcaaa
    10621 tgtttcacac acaactactc ctgtttctaa ttcattttga tctgctaagc attgagcaat
    10681 ttctgcatga aatcctcgat aaagatttcc tccaccaaaa tgaagccata ctggatgttg
    10741 ctggccttct tgcttaactt ctttgacatc aaaacacggc agtagaatct gcatttcatt
    10801 gaattcttcg atagaatttt gccaattttt aatcgaaacc ataattaacc tcctcaaact
    10861 gatatgttag ttttgataca ttttatattt taaaacccat tgtaaccgtt gtcaatataa
    10921 aaagggaata aacttgcttt tttctaccat atcagaatga ttgtattttt tattcagaaa
    10981 aataaaatac tccttctttt tggactgatt ttatcgaaac ttattttttc tttttttcat
    11041 gtattctttt atttttataa ctagtgtatt acttcaaaat ttgatattat atggcatgaa
    11101 cagaattaaa atatcagtta aaataaaagt taaggagcat tgtgtatgac aattaaaact
    11161 agtctccaaa atcaagctta ccaatctatt cgccgacaaa ttatctatgc tgacttagaa
    11221 cctggaaaaa aagtatcaga aaaaaaatta gaagaaacac tttctattgg tcgtacacca
    11281 atacgtgaag cactgattca actccgacaa caaggtcttg ttgatacaat tcctcaaagc
    11341 ggaaattata tctcattaat tgatttagat tcggccaaaa atgctcgatt tgtcagagaa
    11401 catttggaac gacaaattat gttagagtgt tgtgccaaaa tgaataacca aaaaaaacgg
    11461 attttagaaa ctataatttc agaacaagaa aaagctgtta gtcaaaaaaa tgctcgtagt
    11521 ttttttcata atgataattt attccataaa gcttgttttg aaattgcaga gcgagaagaa
    11581 gtatggcaat ggttagaaaa taacaataca cacttagaac gatttcgatg gctacgagta
    11641 aaaacggaag gattaaagtg ggaaaaaatt atggaacaac attatcaact atttgaggca
    11701 ttaacaaaac aagatccaga agaagctaac tttctaacat cgttgcatct tcatttaatg
    11761 cttaatgagc aaaatattgt tttagctact catccaaaat atttcaaaaa tatataactt
    11821 ttaactcttt ttaaattaaa tggttgttct gatcagaaaa atatcgcttg ttttcttatt
    11881 atgtaatata tttcataaac tattctgcat aacaactatt gtttgtactt tttacattgg
    11941 ataactaatt atgactgctg ataacttttg atattaaact agtatctcca atacaaataa
    12001 aaaatagata tcttgttcat caaaattagt tcgtggatac ttaccttcat ttacatcatt
    12061 cagcggtttc ccattttctt ttattctatt atttcatttt ctaatagact tggcttcatt
    12121 tcgcaatact ttttttcaat aggcatcaaa ttggtgttgt ttagatattt agttgtagtt
    12181 ttgtgacatg ataagtatgc tcctttttct cttttccact aatacaacga tcaaagggtg
    12241 cttttttcaa aaaactagaa aaatcaaaat aagattcgta ttttgattct atatatcctg
    12301 tttcaactac atcttatatt tcacaataga ataaatagcc ttttgcatag ctgcttaact
    12361 tataagaaat tgcaatttac ttcttcaaag tgaaaacgaa tatgagtaaa ataaaattat
    12421 tcttcttaca gataattaaa aatagcgcgt aacaaaaatc ggcattcaat cacataatta
    12481 ggattgatgc cgattactta tattctacgt atagttttag tattcttatt ttttatcacc
    12541 agtaaccaat tgagaacagc cctaaagcag aatagtcttt tatctcaaaa cgcttaattt
    12601 caggcttagg aatatgaata atcttttgag tatattcttt ctcttttaat cttactgaag
    12661 aaataccact gc
//
LOCUS       DAFGZE010000083        12309 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.086, whole genome shotgun sequence.
ACCESSION   DAFGZE010000083 DAFGZE010000000
VERSION     DAFGZE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.086"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LTU54_002430"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="LTU54_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBL8297122.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LTU54_002431"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="LTU54_002431"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBL8297123.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAVDFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="LTU54_002432"
     CDS             complement(2169..2906)
                     /locus_tag="LTU54_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL8297124.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="LTU54_002433"
     CDS             complement(2997..3524)
                     /locus_tag="LTU54_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297125.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="LTU54_002434"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="LTU54_002434"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBL8297126.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="LTU54_002435"
     CDS             complement(4401..4886)
                     /locus_tag="LTU54_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBL8297127.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="LTU54_002436"
     CDS             complement(5039..5236)
                     /locus_tag="LTU54_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297128.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LTU54_002437"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="LTU54_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HBL8297129.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LTU54_002438"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="LTU54_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HBL8297130.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="LTU54_002439"
     CDS             9211..9423
                     /locus_tag="LTU54_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HBL8297131.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="LTU54_002440"
     CDS             9425..11104
                     /locus_tag="LTU54_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBL8297132.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="LTU54_002441"
     CDS             complement(11358..11558)
                     /locus_tag="LTU54_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBL8297133.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11869)
                     /locus_tag="LTU54_002442"
                     /pseudo
     CDS             complement(11708..11869)
                     /locus_tag="LTU54_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         3590 a         2654 c         2131 g         3934 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcaacggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa taacattacc agaaggataa tataggttag
    11881 tacacgtata aaaatatctt ttatattgac acaatttatt taacaagtat aataataatt
    11941 gaaagtgaat ttaaacaaat atacaccata aacacaacgt acctgcgaat acgttgtgtt
    12001 tttttctttt tcaaaaaaag acgcttaccg ctgcgaaaca gtaagcgcca ttgtcggctc
    12061 taatgtttat ctagccaatg actaaataat gccaaagtta aaccaattaa caatggcaag
    12121 agcacgtgct ccaaagtgaa ttcaaacaaa tcatacacct cctcccacga aaatctctgt
    12181 ggaagcgacg agccgatatt ttattatacc attattgaat tttatttttt atcaaataga
    12241 ttaaattatg taccctgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
    12301 tgaccaact
//
LOCUS       DAFGZE010000084        12291 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.087, whole genome shotgun sequence.
ACCESSION   DAFGZE010000084 DAFGZE010000000
VERSION     DAFGZE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.087"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(177..734)
                     /gene="frr"
                     /locus_tag="LTU54_002443"
     CDS             complement(177..734)
                     /gene="frr"
                     /locus_tag="LTU54_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBL8297134.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(737..1459)
                     /locus_tag="LTU54_002444"
     CDS             complement(737..1459)
                     /locus_tag="LTU54_002444"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBL8297135.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1595..2476)
                     /locus_tag="LTU54_002445"
     CDS             complement(1595..2476)
                     /locus_tag="LTU54_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBL8297136.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2575..3357)
                     /gene="rpsB"
                     /locus_tag="LTU54_002446"
     CDS             complement(2575..3357)
                     /gene="rpsB"
                     /locus_tag="LTU54_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBL8297137.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3716..4195
                     /locus_tag="LTU54_002447"
     CDS             3716..4195
                     /locus_tag="LTU54_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBL8297138.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4412..5503
                     /gene="phnW"
                     /locus_tag="LTU54_002448"
     CDS             4412..5503
                     /gene="phnW"
                     /locus_tag="LTU54_002448"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBL8297139.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5496..5624
                     /locus_tag="LTU54_002449"
     CDS             5496..5624
                     /locus_tag="LTU54_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL8297140.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGTQLTDKEIR"
     gene            5628..6173
                     /locus_tag="LTU54_002450"
     CDS             5628..6173
                     /locus_tag="LTU54_002450"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HBL8297141.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(6627..8318)
                     /locus_tag="LTU54_002451"
     CDS             complement(6627..8318)
                     /locus_tag="LTU54_002451"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBL8297142.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="LTU54_002452"
     CDS             complement(8738..9685)
                     /gene="arcC"
                     /locus_tag="LTU54_002452"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBL8297143.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9800..10819)
                     /gene="argF"
                     /locus_tag="LTU54_002453"
     CDS             complement(9800..10819)
                     /gene="argF"
                     /locus_tag="LTU54_002453"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBL8297144.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="LTU54_002454"
     CDS             complement(10910..12139)
                     /gene="arcA"
                     /locus_tag="LTU54_002454"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBL8297145.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3373 a         2541 c         2210 g         4167 t
ORIGIN      
        1 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
       61 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
      121 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
      181 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
      241 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
      301 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
      361 tttgcatttt ctgcttcttt tttcacatct ttagctaatt cttttctgcg ttcttctgtc
      421 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
      481 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
      541 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
      601 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
      661 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
      721 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
      781 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
      841 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
      901 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
      961 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
     1021 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
     1081 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
     1141 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
     1201 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
     1261 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
     1321 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
     1381 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
     1441 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
     1501 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
     1561 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
     1621 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
     1681 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
     1741 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
     1801 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
     1861 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
     1921 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
     1981 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
     2041 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
     2101 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
     2161 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
     2221 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
     2281 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
     2341 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
     2401 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
     2461 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
     2521 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
     2581 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
     2641 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
     2701 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
     2761 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
     2821 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
     2881 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
     2941 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
     3001 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
     3061 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
     3121 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
     3181 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
     3241 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
     3301 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
     3361 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
     3421 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
     3481 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
     3541 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
     3601 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
     3661 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
     3721 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
     3781 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
     3841 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
     3901 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
     3961 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
     4021 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
     4081 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
     4141 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
     4201 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
     4261 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
     4321 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
     4381 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
     4441 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
     4501 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
     4561 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
     4621 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
     4681 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
     4741 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
     4801 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
     4861 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
     4921 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
     4981 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
     5041 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
     5101 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
     5161 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
     5221 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
     5281 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
     5341 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
     5401 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
     5461 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
     5521 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
     5581 ttctagaaaa aggaacccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
     5641 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
     5701 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
     5761 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
     5821 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
     5881 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
     5941 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
     6001 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
     6061 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
     6121 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
     6181 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
     6241 aacagaatac attttgtacg ctgacaagaa tccagaatga cttttataca aaataagagg
     6301 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
     6361 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
     6421 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
     6481 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
     6541 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
     6601 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
     6661 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
     6721 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
     6781 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
     6841 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
     6901 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
     6961 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
     7021 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
     7081 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
     7141 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
     7201 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
     7261 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
     7321 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
     7381 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
     7441 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
     7501 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
     7561 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
     7621 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
     7681 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
     7741 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
     7801 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
     7861 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
     7921 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
     7981 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
     8041 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
     8101 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
     8161 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
     8221 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
     8281 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
     8341 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
     8401 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
     8461 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
     8521 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
     8581 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
     8641 agtcgtttca taaaaaaaaa atccagatgg cctaaaggag aagaaatacc acctggatag
     8701 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
     8761 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
     8821 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
     8881 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
     8941 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
     9001 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
     9061 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
     9121 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
     9181 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
     9241 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
     9301 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
     9361 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
     9421 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
     9481 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
     9541 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
     9601 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
     9661 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
     9721 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
     9781 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
     9841 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
     9901 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
     9961 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    10021 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    10081 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    10141 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    10201 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    10261 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    10321 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    10381 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    10441 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    10501 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    10561 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    10621 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    10681 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    10741 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    10801 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    10861 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    10921 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    10981 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    11041 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    11101 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    11161 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    11221 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    11281 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    11341 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    11401 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    11461 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    11521 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    11581 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    11641 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    11701 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    11761 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    11821 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    11881 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    11941 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    12001 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    12061 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    12121 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    12181 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    12241 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt t
//
LOCUS       DAFGZE010000085        12129 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.088, whole genome shotgun sequence.
ACCESSION   DAFGZE010000085 DAFGZE010000000
VERSION     DAFGZE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.088"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..1342
                     /locus_tag="LTU54_002455"
     rRNA            <1..1342
                     /locus_tag="LTU54_002455"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..1552
                     /gene="rrf"
                     /locus_tag="LTU54_002456"
     rRNA            1437..1552
                     /gene="rrf"
                     /locus_tag="LTU54_002456"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            1566..1638
                     /locus_tag="LTU54_002457"
     tRNA            1566..1638
                     /locus_tag="LTU54_002457"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1599..1601,aa:Val,seq:tac)
     gene            1642..1714
                     /locus_tag="LTU54_002458"
     tRNA            1642..1714
                     /locus_tag="LTU54_002458"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1675..1677,aa:Lys,seq:ttt)
     gene            1722..1803
                     /locus_tag="LTU54_002459"
     tRNA            1722..1803
                     /locus_tag="LTU54_002459"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1756..1758,aa:Leu,seq:tag)
     gene            1821..1893
                     /locus_tag="LTU54_002460"
     tRNA            1821..1893
                     /locus_tag="LTU54_002460"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1854..1856,aa:Thr,seq:tgt)
     gene            1905..1976
                     /locus_tag="LTU54_002461"
     tRNA            1905..1976
                     /locus_tag="LTU54_002461"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1937..1939,aa:Gly,seq:gcc)
     gene            1993..2078
                     /locus_tag="LTU54_002462"
     tRNA            1993..2078
                     /locus_tag="LTU54_002462"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2027..2029,aa:Leu,seq:taa)
     gene            2089..2162
                     /locus_tag="LTU54_002463"
     tRNA            2089..2162
                     /locus_tag="LTU54_002463"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2123..2125,aa:Arg,seq:acg)
     gene            2172..2245
                     /locus_tag="LTU54_002464"
     tRNA            2172..2245
                     /locus_tag="LTU54_002464"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2206..2208,aa:Pro,seq:tgg)
     gene            2265..2337
                     /locus_tag="LTU54_002465"
     tRNA            2265..2337
                     /locus_tag="LTU54_002465"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2298..2300,aa:Ala,seq:tgc)
     gene            2365..2438
                     /locus_tag="LTU54_002466"
     tRNA            2365..2438
                     /locus_tag="LTU54_002466"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2399..2401,aa:Met,seq:cat)
     gene            2452..2525
                     /locus_tag="LTU54_002467"
     tRNA            2452..2525
                     /locus_tag="LTU54_002467"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2486..2488,aa:Ile,seq:cat)
     gene            2549..2638
                     /locus_tag="LTU54_002468"
     tRNA            2549..2638
                     /locus_tag="LTU54_002468"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2585..2587,aa:Ser,seq:tga)
     gene            2651..2724
                     /locus_tag="LTU54_002469"
     tRNA            2651..2724
                     /locus_tag="LTU54_002469"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Met,seq:cat)
     gene            2743..2818
                     /locus_tag="LTU54_002470"
     tRNA            2743..2818
                     /locus_tag="LTU54_002470"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2776..2778,aa:Asp,seq:gtc)
     gene            2820..2890
                     /locus_tag="LTU54_002471"
     tRNA            2820..2890
                     /locus_tag="LTU54_002471"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2852..2854,aa:Gly,seq:tcc)
     gene            2904..2977
                     /locus_tag="LTU54_002472"
     tRNA            2904..2977
                     /locus_tag="LTU54_002472"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2938..2940,aa:Ile,seq:gat)
     gene            3002..3089
                     /locus_tag="LTU54_002473"
     tRNA            3002..3089
                     /locus_tag="LTU54_002473"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3036..3038,aa:Ser,seq:gct)
     gene            3111..3182
                     /locus_tag="LTU54_002474"
     tRNA            3111..3182
                     /locus_tag="LTU54_002474"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3144..3146,aa:Glu,seq:ttc)
     gene            3205..3294
                     /locus_tag="LTU54_002475"
     tRNA            3205..3294
                     /locus_tag="LTU54_002475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3241..3243,aa:Ser,seq:tga)
     gene            3307..3380
                     /locus_tag="LTU54_002476"
     tRNA            3307..3380
                     /locus_tag="LTU54_002476"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3341..3343,aa:Met,seq:cat)
     gene            3399..3471
                     /locus_tag="LTU54_002477"
     tRNA            3399..3471
                     /locus_tag="LTU54_002477"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3432..3434,aa:Asp,seq:gtc)
     gene            3475..3547
                     /locus_tag="LTU54_002478"
     tRNA            3475..3547
                     /locus_tag="LTU54_002478"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3508..3510,aa:Phe,seq:gaa)
     gene            3552..3622
                     /locus_tag="LTU54_002479"
     tRNA            3552..3622
                     /locus_tag="LTU54_002479"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3584..3586,aa:Gly,seq:tcc)
     gene            3635..3708
                     /locus_tag="LTU54_002480"
     tRNA            3635..3708
                     /locus_tag="LTU54_002480"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3669..3671,aa:Ile,seq:gat)
     gene            3720..3791
                     /locus_tag="LTU54_002481"
     tRNA            3720..3791
                     /locus_tag="LTU54_002481"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3753..3755,aa:Glu,seq:ttc)
     regulatory      3980..4059
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            4057..5250
                     /locus_tag="LTU54_002482"
     CDS             4057..5250
                     /locus_tag="LTU54_002482"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBL8297146.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            5380..6852
                     /locus_tag="LTU54_002483"
     CDS             5380..6852
                     /locus_tag="LTU54_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8297147.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            6935..7723
                     /locus_tag="LTU54_002484"
     CDS             6935..7723
                     /locus_tag="LTU54_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBL8297148.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(7790..8458)
                     /locus_tag="LTU54_002485"
     CDS             complement(7790..8458)
                     /locus_tag="LTU54_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HBL8297149.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            8573..10285
                     /locus_tag="LTU54_002486"
     CDS             8573..10285
                     /locus_tag="LTU54_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297150.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            10288..12060
                     /locus_tag="LTU54_002487"
     CDS             10288..12060
                     /locus_tag="LTU54_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297151.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         3467 a         2261 c         2910 g         3491 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatatg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg tgagagtagg acgtcgccac gcttaagtta
     1561 tccatggagg tttagctcag ctgggagagc atctgcctta caagcagagg gtcagcggtt
     1621 cgatcccgtt aacctccata tgagccgtta gctcagttgg tagagcatct gacttttaat
     1681 cagagggtca caggttcgag ccctgtacgg ctcatatttt tgcgggtgtg gcggaattgg
     1741 cagacgcact agatttagga tctagcgcct tacggcgtgg gggttcaagt cccttcaccc
     1801 gcataattta agttttacat gccggcttag ctcagttggt agagcatctg atttgtaatc
     1861 agagggtcga gggttcaagt cctttagccg gcatctttaa acacgcggaa atagctcagt
     1921 ggtagagcac caccttgcca aggtgggggt cgcgggttcg aaccccgttt tccgctttgc
     1981 caaagcaatc gcgccggggt ggcggaactg gcagacgcac aggacttaaa atcctgcggt
     2041 gagtgatcac cgtaccggtt cgattccggt cctcggcatt tcaaatatgc gcccatagct
     2101 caactggata gagtgtttga ctacggatca aaaggttagg ggttcgactc ctcttgggcg
     2161 cgttaattaa acgggaagta gctcagcttg gtagagcact tggtttggga ccaaggggtc
     2221 gcaggttcga atcctgtctt cccgataatt ataaatttaa acacggggcc ttagctcagc
     2281 tgggagagcg cctgctttgc acgcaggagg tcagcggttc gatcccgcta ggctccattt
     2341 catttaaata atgaatatct ttttcgcggt gtagctcagc tggctagagc gtccggttca
     2401 tacccgggag gtcgggggtt cgatcccctc cgccgcgatt tttaagagcc tggaccttta
     2461 gctcagttgg ttagagcaga cggctcataa ccgtccggtc gtaggttcga gtcctacaag
     2521 gtccattgtg gcgttgtaag tttcatatgg aggattaccc aagtccggct gaagggaacg
     2581 gtcttgaaaa ccgtcaggcg ggtaaaaccg tgcaagggtt cgaatccctt atcctccttt
     2641 ttcatattat cgcgggatgg agcagtctgg tagctcgtcg ggctcataac ccgaaggtcg
     2701 taggttcaaa tcctgctccc gcaattaaaa actaaaaaca ctggttccgt ggtgtagggg
     2761 ttaacatgcc tgcctgtcac gcaggagatc gcgggttcga ttcccgtcgg aaccgccatg
     2821 cgggtgtagt ttagtggtaa aaccacagcc ttccaagctg ttgtcgcgag ttcgattctc
     2881 gtcacccgct tttagttttt tatgggccta tagctcagct ggttagagcg cacgcctgat
     2941 aagcgtgagg tcgatggttc gagtccattt aggcccatta gtacaaaatc ttattggttt
     3001 tggggaagta ctcaagtggc tgaagaggcg cccctgctaa gggtgtaggt cgggaaaccg
     3061 gcgcgagggt tcaaatccct ccttctccgt ttcaatattt tgttttacct ggcccgttgg
     3121 tcaagcggtt aagacaccgc cctttcacgg cggtaacacg ggttcgaatc ccgtacgggt
     3181 cattaattta atatagtttt acatggagga ttacccaagt ccggctgaag ggaacggtct
     3241 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
     3301 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
     3361 ttcaaatcct gctcccgcaa ttaaaaacta aaaacattgg ttccgtggtg taggggttaa
     3421 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gtttggctcg
     3481 gtagctcagt tggtagagca atggattgaa gctccatgtg tcggcagttc gattctgtcc
     3541 cgcgccatta tgcgggtgta gtttagtggt aaaaccacag ccttccaagc tgttgtcgcg
     3601 agttcgattc tcgtcacccg cttttagttt ttatgggcct atagctcagc tggttagagc
     3661 gcacgcctga taagcgtgag gtcgatggtt cgagtccatt taggcccatt tattttattg
     3721 gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg gttcgaatcc
     3781 cgtacgggtc atggaagata ctttttgagt atctttttct ttttatctga aagaatgatt
     3841 ttaaaaagct tagcagagtt atctttatgg atgattgtgc taagcttttt tggacagttc
     3901 taatgtaaaa tatttttata ttttttaagc aaaaaaatga aaaaatacga gcagaaaact
     3961 gttttatctg ctatacttgg ttcgttgcaa gttcccgaaa ggatccgcag gacggagatg
     4021 ccttgtaacc gaatttaact agggggaatc attataatgg tagaaagaca cttatttaca
     4081 tcagaatctg tttctgaagg acatccagat aaaattgctg accaaatcag tgatgcaatc
     4141 ttggatgcaa ttttaaaaca agatccaaca gcacgagtag cgtgtgaaac gtctgtaaca
     4201 actggtcttg ttttagtatt tggagaaata tctacgacgg catatgtaga tattcaaaaa
     4261 gtcgtacggg aaacaataaa agaaattggt tatacacgtg caaaattcgg atttgatgga
     4321 gatacagcag ctgtattggt tgcgattgat gaacaatctc ctgatattgc tcaaggagtt
     4381 gatgaagcac ttgagatccg ggatgaagat aaaaaagacg tattagatga gattggtgct
     4441 ggtgaccaag gtttaatgtt tgggtttgct gtagatgaaa caccagaatt gatgccttta
     4501 ccaatcgctt tgagtcatcg tctggtacga cgcttggcag atttgcgtaa atcaaatgaa
     4561 ttaacgtatt tacgtccaga tgcaaaatct caagtaacgg ttgaatatga tgatcaagga
     4621 caaccggaac gcgtagatac aatcgttatt tcaacacagc atgatgatgc agtagataat
     4681 gaaacaattc gtcatgatgt catcgaaaaa gtagtgaagg aagttattcc agctgaatta
     4741 ttagatgatc aaacgaaata ttatatcaat ccgactggcc gatttgtcat tggtggtcct
     4801 caaggggatg ccggattaac aggaagaaaa atcattgttg atacgtatgg cggttatgct
     4861 cgtcatggtg gcggtgcttt ttctggtaaa gatgcgacaa aagttgaccg ttctgccagc
     4921 tatgctgcac gttatattgc taaaaacatt gttgcagcag ggcttgctcg caaagcagaa
     4981 gtacagctag cttatgcgat tggtgttgct caacctgttt cgatctcaat caatactttc
     5041 ggtactggaa cggttccgga agaagaactg attgctgcag tgagagaaaa cttcgatctt
     5101 cggccagcag gaatcatcga gatgcttgat ttgcgtcgtc cgatttacaa acaaacagct
     5161 gcttacggtc attttggtcg tacagatgta gatttgcctt gggaacatac ggacaaagta
     5221 gatgcgttga aagcaagttt agcaaaataa gttatattca gctggaacga tcgccaagtg
     5281 gtggttgttc cagctttttt tatggcagtt aacctcttga aaaagatagt aattatttat
     5341 ctatttttag atcctgtcat aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt
     5401 actttggtca cagtgagtat tttcattgca acgttcatga cggctattga aggtacaatc
     5461 gtaacaaccg ctatgccaac gattgtcggt tcattacatg gaatggaaat catgaactgg
     5521 gtattttcga tttatttgct gacaaacgct atgcttactc cgatttacgg aaaacttgcg
     5581 gataaagtgg gacggaaacc aatctttatt tttgggacat tattgtttat tatcggttcg
     5641 ttattttgcg gtttatctca aagcatgatt gggttgattg ctgctcgtgc gattcaaggg
     5701 atgggtgctg gggcaatgat gccagtagct ttgacgatca tcggcgatct ttattctatt
     5761 gaaaagcgag caaaagtttt aggtttgaat agttcagcct gggggattgc cagtgttttt
     5821 ggaccgcttg cgggaggatt catcgttgaa acgatcagct ggcactggat cttttttatc
     5881 aatgtgccga tcggaattct attaattatt ttgatttggc tatatttaaa tgaaccaaaa
     5941 atcatacatg agtctaaacc aatggatatt tttggtagtt ttagtttgat ggccatgctt
     6001 gtctcattgc tgttagggtt ccaattatta ggagacgaag gaatgagtcc gcttgtttct
     6061 gctttgctgc taggtagtat cgtaatgtta ggtatttttg tttttgccga aaaaagagct
     6121 aaagatcctg tcatcatgat gcatttattc aaaaatccac tatttgttgt ggtcaacttg
     6181 gctgcagcat tggctagtgg ttttttgatg ggaatcgatg tttatatccc tatgtggatg
     6241 caaggtgtat tagggctgaa tgcagcaatt ggtgggctgg tattagcacc attatcacta
     6301 gtctggatgg tcggttcttt ccttgcaagc aagtggatga ctcaaatgaa aactcattgg
     6361 gtcttgagaa ttggactact aatcgcagct attgggggcg cttggttgta tcttatgcct
     6421 ttcagaagta actatttatg gttttttgct atatcaacgg tcatcggtat aggcttaggt
     6481 gtgaccatga ctacctcgac tgtcagtgca caaaatagtg tatctcgtga ccagcttggt
     6541 gtagcaactt cttttaatac gttagttcga acaattggtc aaacagtcat ggtttctatt
     6601 tttggtttat tgataaatgg ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat
     6661 gatgcagata tcatgaataa actggtgaat ccacaaacgg ctaaatatat tgatgcggat
     6721 attttgttac cattaagaaa aattctttat gatgggttac attccgttta tctagttggc
     6781 ttgttactcg tgatcattgc tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa
     6841 ataaaacagt agtttcggtt aagatatgta cgtagccgaa tcattcaaat ttaaagttat
     6901 tcaaaaataa cttcttacta aggaaggcga aaatatggca tttattcaag caaatattta
     6961 ttcgaatgtg ttagaaatgg aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa
     7021 ggtgattggc accagttcag agcaagagac aacggatata cctgttttat atttacttca
     7081 tggaatgggt ggcaatcata gtgtatggga aagacgtacg tcgattgaac gttatgtagc
     7141 aaactacgga ttagctgtca tcatgccatc tactgatctt ggctggtaca cagatacagt
     7201 ttatgacatg aagtattgga cattcattgc agaagagcta cctgagattt gtcatgaatt
     7261 attcccgcaa ttaacaagaa aaagagaaaa aacatatgcc gctggattat cgatgggcgg
     7321 atatggcgcg ttaaaactag gccttgcaaa atcagaaaat ttcgcagcag taatctctct
     7381 ttcaggcgct gtttctgtag gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc
     7441 tttttgggaa gggatctttg gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca
     7501 atacttattg aaagaactag taaaaagcgg aaaaccagtt ccaagatttt atatggcttg
     7561 tggggaatct gattttcttt atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca
     7621 aggcttggcg gtcacctttg aacaaggacc tggcgatcat aactggatct tctgggacca
     7681 atggattcaa cgtgcattgg cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa
     7741 gatagctcct atagagatca caaacatagt gatctctata ggagcttttt tactgctcgt
     7801 tttctcttct tttattttgt tggcataagc cttcaatata ggtttgtgca ctcgttagaa
     7861 agagtgcttt ttcattcaat tctgcatggg actgcatact ttctttaaat ccctctaatt
     7921 gattttcaac tacataatta cgcataaata gaactgtttc tttcaaaaat tcatttccgt
     7981 ccataatttt ttgtcgaagc tgctgttcat gctgctgatc cattaactgt ccaatcaata
     8041 aattgtggag agaggcaatg gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa
     8101 gtaattggat catttgctcc aaatgttcta atcgactcgg aaaagcgggc acagtattga
     8161 tttctaattc tgccccacaa gggaaacgtg tataaaggtc atagtaattt tccatatact
     8221 gcagcaactg ttcattccaa ggcaaagttg gatctggaag cgctaattct tcttccattg
     8281 tttcagccat tttttgcaag agctgttgtt tgtttttgaa ataccaatat aaagcaggtg
     8341 cttgaacatg aattctgttt gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt
     8401 cagccaggat atcaaaggcg gcctgaatga tgattgcttg agaaagcttt atttccattt
     8461 tatcaacctc gtgtattgca ttatcttttt aaacatggta aattgttagt cggtctaatt
     8521 ttacagtgtt aaattagtcc tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt
     8581 aatcttacga catgcgaaaa aatataaagt agctgttttt atttccttgc tgtccgtagc
     8641 agtcatggtt gcagcagcat tatggcaacc taagttacta cagcaagtat tggaagctat
     8701 tattacagaa gataatgatg aaatgaaaac aattggtatt tacctgatca gtctggcttt
     8761 gctaggatta gccgcaggtg taacgaacac gattttctct gcaaaagtag ctcaaggcgt
     8821 aagtgcagat atccgagaag aagcatttcg aaagatccag acattttcat ttggaaatat
     8881 cgaacagttc tctgctggaa atcttgttgt tcgtctaaca aatgatatca cgcaaatcca
     8941 aaatttagtg atgatttctt tgcaatcact ttttcgaatt ccattcttat ttattggtgc
     9001 cttcatttta gctatgatca cgatgccgca attatggtgg atcatcgtag cgcttatcgt
     9061 tacggtattt ctgattacgg cactttcatt tacaaggatg ggcaagcact tcatgatcat
     9121 tcaaaaactg attgatcgtg tgaatagcat tgcaaaagaa aacttactag ggattcgtgt
     9181 agtgaaatct tttgtacaag aagaaaacca attgaagtca ttcagcaaag taagtgagga
     9241 cttaacaaaa cacaacatca tagtcggtac attattttct gtgatgatcc cgtccttcat
     9301 gctggctgcc aatttggctg tggttggggc gatctttttt gtgagcgatc ttgttaaaga
     9361 tgatcctacg ttaattggtg gaattgcttc attcatgaac tacttgatgc agattatgat
     9421 ggcaatcatt attggcggaa tgatgatgat gatgacttca agagcagccg tatcgctgaa
     9481 gcggattgcg gaaattttag atgctgaacc agatcttact tatttagacg ttccagaaca
     9541 agagttgacg ggttctgtca cttttgacca tgtttctttt cgttatcctg gtgaggatac
     9601 agatacgctt aaagatatca gcttttctat caaacctgga gaaatggttg gtatcgtcgg
     9661 ggcgactggt gccgggaagt caacactagc acaattgatc cctcgtctat ttgatccgac
     9721 agaaggcaaa gttgaagtag gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag
     9781 acaaacggtt tcctttgttt tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa
     9841 cttgcgccaa gggaaaaaga atgcaacaga aaaagagatg gaacatgctt cttctattgc
     9901 tcaagcaaaa gaattcatcg aaaagttatc tgatcgttac gaagcaccaa tagaagaacg
     9961 tagcagtaac ttttctggtg gtcaaaagca acgtttatcc attactcgtg gcgttatcgg
    10021 taatccaaag atcttgatcc tagatgacag tacgagtgca ctggatgctc gctctgaaaa
    10081 gctggtccga gaagcactcg atcgagattt gaagggaaca acgacgattg tcatcgcaca
    10141 aaaaattgct tctgttgtca aagcagaccg catcttagta ttggatgaag gcagattagt
    10201 cggcgaagga acccatgagg aattggtagc tggaaaccgt gtctatcagg aaatctttga
    10261 aactcaaaaa ggaacggagg agtaagtatg actgatctaa taaaagcaag taaatttttc
    10321 tatcactatt tgaagcggta caaactctcc tttttcttta tctttatcac agtgatcatt
    10381 gctacctatc tacaagtaaa ggcacctcaa tacatcgggg aagcttttca agaattagct
    10441 aattacgtag gaggcttgat gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata
    10501 tggaaactct tgctgtttta tgtactagct agtgcagcca actttatcta tagtattttg
    10561 ttcacacaag tagtagggaa gtcaacgaac cgaatgcgta tcggtctatt caacaaattg
    10621 gaaaaattga cgatccgttt ctttgattct catcaagacg gtgaaatttt aagtcgtttt
    10681 accagtgact tagataatat tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg
    10741 aatgctgttt tacttgtcgg gatcttgatc atgatgttcc gccaaaatgt agagttagct
    10801 tgggctacaa ttgcttccac tccagtagcc gttttgatag ctgtcttgat tatccgcaaa
    10861 gcacggaaat atgtggatct tcagcaagat gaagtgggga aactaaatgg ttatatggat
    10921 gaaaaaatca gcggacaacg tgtcatcatc acaaatggct tacaagaaga aacgattgaa
    10981 ggattcttag agcataatca aagtgttcgc gaagcaacct tcaaaggaca agcttattct
    11041 ggtctactat ttccgatgat gcaaggaatg tcactggtca atacagcgat agttatcttt
    11101 ttcggcggct ggttagcatt aaatggtgat ttggaacgtt cggtggcatt aggcttagtt
    11161 gtgacattcg tccagtattc acaacaatat taccaaccac tgatgcagat ttcatctgga
    11221 tacagcatga tccaattagc gattacaggg gcaagacgat tgaatgaaat atttgatgaa
    11281 aaagatgaag tcaaaccaga aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg
    11341 ttgcagcacg tagattttgg ttattctcca gaaaaactag tcttgaaaga tgtctcgatc
    11401 aatgtggaca aaggagaaat ggttgctctt gttggtccaa caggatcggg gaaaacgacg
    11461 atcatgaatc tgctaaaccg tttttacgat gtcaatgatg gttcagtagt catcgatgga
    11521 atagatatcc gagattatga tcttgatgca ttacgtagac atgtaggaat cgttttgcaa
    11581 gaatctgtat tgttctccgg aacgatcaaa gacaatatca cattcggcaa accggacgct
    11641 tcagatgaag aagtcatcaa tgcagcaaaa caagcgaata ttcatgactt catcatgacg
    11701 ttagaaaaag gttatgatac agagatatcc gaagaaaata acttattcag tacaggtcaa
    11761 aagcagttga tcagtattgc tcgaacgatc atcacaaatc catctttatt gattttggat
    11821 gaagcaacaa gtaatgtaga cacggtaaca gaagcaagga tccaaaaagc aatggatgaa
    11881 gcaatcaaag gaagaaccag ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca
    11941 gatagaatcg ttgtcttgcg tgatggggaa gtcattgaag aaggcagtca tcatgagtta
    12001 ttagcagaaa atggatttta tgcggaactt tatcataatc aatttgtatt tgaaagttaa
    12061 attaaaagaa cctcgttata acggggttct ttttatatat tttatgttgt ttgtttttat
    12121 aaggggcag
//
LOCUS       DAFGZE010000086        12023 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.089, whole genome shotgun sequence.
ACCESSION   DAFGZE010000086 DAFGZE010000000
VERSION     DAFGZE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.089"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            960..1928
                     /locus_tag="LTU54_002488"
     CDS             960..1928
                     /locus_tag="LTU54_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL8297152.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="LTU54_002489"
     CDS             1945..2469
                     /locus_tag="LTU54_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL8297153.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2845..3126
                     /locus_tag="LTU54_002490"
     CDS             2845..3126
                     /locus_tag="LTU54_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297154.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3128..3544
                     /locus_tag="LTU54_002491"
     CDS             3128..3544
                     /locus_tag="LTU54_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297155.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            4166..5320
                     /locus_tag="LTU54_002492"
     CDS             4166..5320
                     /locus_tag="LTU54_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HBL8297156.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            5314..5736
                     /locus_tag="LTU54_002493"
     CDS             5314..5736
                     /locus_tag="LTU54_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297157.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            6047..6427
                     /locus_tag="LTU54_002494"
     CDS             6047..6427
                     /locus_tag="LTU54_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBL8297158.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            6409..7323
                     /locus_tag="LTU54_002495"
     CDS             6409..7323
                     /locus_tag="LTU54_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBL8297159.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            7283..7561
                     /locus_tag="LTU54_002496"
     CDS             7283..7561
                     /locus_tag="LTU54_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297160.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            7568..8257
                     /locus_tag="LTU54_002497"
     CDS             7568..8257
                     /locus_tag="LTU54_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297161.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(8378..8464)
                     /locus_tag="LTU54_002498"
     CDS             complement(8378..8464)
                     /locus_tag="LTU54_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL8297162.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            9101..10045
                     /locus_tag="LTU54_002499"
     CDS             9101..10045
                     /locus_tag="LTU54_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBL8297163.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            10066..10599
                     /locus_tag="LTU54_002500"
     CDS             10066..10599
                     /locus_tag="LTU54_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HBL8297164.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            10614..10784
                     /locus_tag="LTU54_002501"
     CDS             10614..10784
                     /locus_tag="LTU54_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297165.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            11239..11463
                     /locus_tag="LTU54_002502"
     CDS             11239..11463
                     /locus_tag="LTU54_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HBL8297166.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            11483..11770
                     /locus_tag="LTU54_002503"
     CDS             11483..11770
                     /locus_tag="LTU54_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBL8297167.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         4578 a         2039 c         2084 g         3322 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttacttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa accagcaatt taaattaata aaaattgctg gttttattct tctttttgct
     2521 tttgcgagca gagcaacatg gacccatgtt gcgatatttt ggtggcgccc caatccccgt
     2581 tttttcgttg aatcttactc ggactgcact cgttttacag ctgatttttc tgaattttga
     2641 aagagcataa ggaaacgtgg tatcactttc ttacgaaagg tgctagatag gctgtgccta
     2701 tcacgtttct cagaaaaaaa tttgctatca cttttttgcc attgtggcta ggctatgtat
     2761 agtgttttgg cgaaaaatag ctatacaaat actatatttg tatggtatac ataaattata
     2821 cgctgatacg aaaggaaaat ttccatggac attttagttc gaaatatcaa tcccaaaatt
     2881 attgcaagaa tagataaacg ttgtcaggaa ctcacaacca aaacgggcaa aaaatggagt
     2941 cgaaataaat atctaaaagt tctaatcgaa agtgattttg accgtcaact tatgaattac
     3001 aaaaaagatc aattcgattt attactcgaa aaatttattg ccattcaaac agaaaatacg
     3061 aacatactga atgactatgt tcacacaaac aaccaactca ttgaaacact tatcggagga
     3121 atataacatg agaaaaactt ctgttcaaaa acacgtaacc ctttatccca gtcacgttga
     3181 cgttttagat tccatcatcg aaaaaaatcc agacgtcaac aattattcag aagccattcg
     3241 ttttttatgt atgagttatg aacctgatac caatacaacc acaaccagta accaagcaaa
     3301 actcaatgcc atgggaaaag aaatttctat catgacagaa atcatggcag aatttgctga
     3361 tttatatcta aaagatactg gtgttcttat cggtacaaat tctaaagtgt atcaagaagt
     3421 gaaagaaaaa attgaaaatc aactcaatcc ttcccgacga aatcaaccca aaaatcgtca
     3481 aaaagaaacc aatcaaacct ttacttctgg aaattttgac ccaacaaact ttctaaaaag
     3541 ataaaaaaag aaaccgatcc ctaaaaaaat tcctcgattt ctttttaggg atcagtcttc
     3601 cagcaagatt tgcaagttgg taccaacttg cgaaaaaaaa gaaaaatccg cttatgcaac
     3661 ttcttctttt tgtattttga ggcgctaccc caatccccaa gtatcggtta tttaatagcg
     3721 cggaaatcca aagaatagga cgtaccctga aacaaaaaaa gactgctctc acgagcagcc
     3781 caaaatgaga ttatcctatt catcaacata aaaatattac aaagtgaaga actagagaaa
     3841 gacaaaaaaa gcatatcaaa tcctctcaaa aataacaagt ctttaaaaaa tgacagataa
     3901 aaatgcacaa agaactaaaa aagagaaatc gcgtaaaatc aagcattaga agctagtaac
     3961 tagctagacc aattaaaaat cacaaagtga gcgtctctat ccattggtag aggggctact
     4021 tggcatagtg tacattatgt taactaacgc tataaataac gattatgttc ccaaaacaat
     4081 attttatgaa ctataacaaa aatttgttat cgcatattgt atattttaat tataggtgta
     4141 taccacttaa atttaacaca aaggcgtgat tattttgaaa cccaaaaaga aaatttatct
     4201 aggtctcctc ttttccttga tgattggaat ttttagtata ggaattacaa atacctatgc
     4261 ttcagaaaat tctaatttca atatcaactc ccaaccaatt gaagaacaag caattaattt
     4321 tgcgaaaact gattttgaac actccataac ttcaattctt ttaatggaaa ataacaccga
     4381 aactgatatt aactatactc ttggttcgcc atttaaaatg ctcgatggag aaaatacggc
     4441 aaatagttct attttctttc cagtattttc agacgctgat aatacgatta aatatattta
     4501 taccgtcaaa aaaaatacaa atggagaata ttcaggaagt atttcacaat ttttagcaaa
     4561 agaacttcaa aatctagtag atagtaatat aacagataca accttatttt ttcaagataa
     4621 tagcatttat tattccgata atggacaaat taaactactt tggaaatcac ctttaagtac
     4681 agaaaaaaat ggtgtttctt cctttacaga aaaaaatgat cttatatccg taaatataaa
     4741 aaataaaatt aaaaatttct ctaaaaaaca aaattttcct gcggttaaac gtgtaaaaag
     4801 tgtctcctcc ggagataact ttattatgat taattggcgt atagcagaaa cccaaacaag
     4861 ttttccgtgg tgtgctgctt acgtagaagc tgctatctta tgtaacaaaa cagatgtacg
     4921 tgctacttca gctagagcca ttatgcatta tacctatcct aatttaaaca acagtcaaat
     4981 tgaatcaaaa agtcttaatc aagatcaaat aataaaatat gcaaattact gtggtgttta
     5041 tcctaaaaaa gtagcacgaa tactaaacct ttcagaggtc caaacacaac taagaaaagg
     5101 aaatgccatt tatatgggaa ctgctggtta tggaaattat agtaacagcc gtcatgcact
     5161 tgcattattt gggtgggtca atcaaggtgg ttatcaaact tactatgtgt ggaatccttg
     5221 gtataactac ccaactgtaa taagtgcaaa tgcaggacca gttacaattc ctgtacctgg
     5281 aggtggtttt acttgggaca ataccattac aaattggtaa ataaacccat tgctttagtt
     5341 atgggtctag tgttagttct tttcttaact agttgttcaa aaaaacaaga aatacaaaag
     5401 actgatctga ttccttttct aggaacttgg caaactaaag atcattcaga acgtctagta
     5461 atcactaatt taaatacttc aaaaaatata gaacaatata ccatcttagt aaaagagaac
     5521 aatcataaaa ttcaaatcat tgataaaaat ttaaatataa atgaacaagt tttcatttac
     5581 aaagcaacaa ataatttact aacactgcgc tctaataaaa aacaattaga ttacactttt
     5641 agtttggatt ctccaaaaaa gctcgtattt attttgacaa cgaatgatgg gacagaaggg
     5701 acagctaaac caattgaatt ttttaaagct gattgatgaa tagttgtttg atattacaaa
     5761 acaataattt caccgaaggt gaaacaagcg aaactttaca aaaagtggct agcatttgct
     5821 agcctttttt gtattgtgga gcgtggtttt tgagggggat tcaaggggga gcttgcgccc
     5881 ctttgacaag cggttatttg caccaaatac gtaggtattt ggctagcaaa tatagagctt
     5941 gccaagccct ttagtgagct ttagtgagct ttacttattg taccttttta ggtacaatgg
     6001 tattgcgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga gcgaacaaaa
     6061 tcaatatttg gctagcaatc attctaaaaa atatacctat cgatccgagc caaaacaaat
     6121 cagttttcga gtgagcgaat ccgaatttgc aaagctgaag cagtcagctg aagctttgca
     6181 aatgagtgtg ccggctttcg tgaaagccaa ggcacaaaac gcgcgtctgg tgacaccgaa
     6241 agtggcgcca gacattgctc aggcaatcgc gcgcgattta gccaaagctg gcgggaatat
     6301 caatcaaatt gccaagtggt gcaacacaca ccaacacgac gttgatcctg gcgacgccca
     6361 acgcttatcc gaaaacctaa aaatcatgca aaaggagcta caaaagatat ggcaacagtt
     6421 aaagtaagcc gaaccacttc ttgtagccga gcgatcaatt acgccgaacc acgagcaacc
     6481 gtcaaaacag ggataaactg tgacattgat tatgccaaaa gcgaaatgaa acaaattcgc
     6541 atgctgtatg gaaaagacga tcgtgtacaa gcacacttat tgatccagtc ttttcgacca
     6601 ggagaaatta ccgctgaaaa agccaatcaa ctaggggcag agtatgcgaa aaaaatagcc
     6661 cctgaccacc aaatcgcgat ttacacacat acagacaaag accatattca taaccacatt
     6721 gtcattaact ccgtcaatct ggaaactggg aacaaatttc aagcccatgg tcaagcattt
     6781 ttaaacaaat gttatgacct aaatgacgag atttgtcttg aacatgactt aacaatcact
     6841 gaacgaggga agaaacccga aaaacgaacc atgtctgaaa tcaaattaaa agaaaaaaat
     6901 gaacctgttt ggaaagacga aattcggttt gcgattgatc aaacaatgaa aaacaaaaaa
     6961 actcgaacgt ataaccaatt ttgtgattct cttaaaacat tcggcattca ttgcttgaat
     7021 cgtgggaaaa attttactta cgaattgatc cacaaaaaga aaaaagtacg atccaacaaa
     7081 ctaggaaagg attacgaaaa ggagacgatt ttacatgagc tggacagacg agaacgagcc
     7141 tacaatcaac aacgaaccaa cgagctacga agagctcgag ctagcattcg cgaaactgca
     7201 aaagaagtac gaagagaaca acgaactctt acgactgaac aaccggaaat tagaccgact
     7261 cgaagaaacc atgaccaaca cgttgaaaga ggagcagact accaacaaga attatcgttc
     7321 taaaacgtta caagacatag aaaatgaaat aaagaaacaa cgtgcttttc ttgcacctta
     7381 taccaaagaa agaatacaaa ctacaattga ttcacaagtc aaaaaattcc accatgcgct
     7441 attaaaccag cgaaataaag aacagaaaaa agtaattatg cagcgatcaa tggatattct
     7501 tgccccctta tgttgcttaa tcatgacact gttagccttt tggctggtat ggggagaata
     7561 aatagaaatg gcattctatt taccctatct actcattttt gccagtattt taggaattat
     7621 ttggcttagc tataaggctt atcaaacaag atattcatta aaaggctcta aaattcgatt
     7681 taagaggttt ttattgccag gggttatatt tacattatca atcgttttaa gtggtttatt
     7741 cggcgtttta gagggaaata aacgtatttc aagaagtatt ttattagggg atatcactca
     7801 aaagtatgaa gcagcatgtg ataaaaagaa aaaggagcaa gtgttaacac aaaaaatgga
     7861 agagtttacc acttgttatg aggaaatgaa cgatattttt gtgaatcaag aaaaacgttt
     7921 gacggataaa aacatggaaa aactcactcg tctttatcaa aacttgccag aaaaacagca
     7981 aaaagaggtt caagacaact atgagcgaac caaaaaagac gtgcaatatt ttaaagatac
     8041 gcagacagaa gaagcttgtt ataatttatt cagtgataca atcccttttt caactagtga
     8101 acaggaacga aaagaacggc aacaatcagt tacctacgaa cgttataaag cactgcttca
     8161 gcaagcaacg aacatacaaa atccaacgaa aaaagaaacg gcattaaatt atctaaaaag
     8221 tgtcaaggaa tggttagatc agcaacagca aaattgaatt tgatcggcaa tcatgttaaa
     8281 ctaaacatgc aaatgatcaa acattagata cacgaaagcc ccagagaggt cggattctct
     8341 ggggcttttt ttcaggttga tgtcgccttt attgatttta cttcttgttt cggttattca
     8401 accaataact gaataaagag acacaaactt ctataagaag ggggcaaatg aacaattcaa
     8461 acattagata cacctccttc cagtcgcaag actgtcggat aggcgacaag ttattatatc
     8521 ttaaaaaatt aaacttgtgt ggtgttttta agtatcgctt attttagggt tgttagggct
     8581 tgccctgacc gcctgtaagg cgctttaaag ctagataagg atttttatcc aagactgtct
     8641 taaaacggct tagataagca ttcagaaccg ctcaaaggcc tctttaccaa catgttttta
     8701 gcaaaggagc ctttaggcga ctgcgcagta caatgtgaga gcgtagcgat cacacctttt
     8761 tggcgaccgt aggaagcaag gttttggatt ggcagttttg aaaaatgcgc gaacctgttt
     8821 aaagaaaaaa gaagtggttt ttaggcggga aaattagccc ccctcttttt atatctcttt
     8881 ttaaacctct ttttaaacct ctttaccgta agctgtgtcc ttactctacc aatggataca
     8941 gaatgttatg aaggacattt tgtctgttta tgaaggacat tttgtctgtt tatgaaggac
     9001 attttgtctg tttatgaagg acattttgtc tgtttgaacg tcaaatatga atgtcttgac
     9061 atttaaaaaa tgtcattcat aattggattg aggtgattat atggctaatg agattgtaaa
     9121 ataccaccat gaattaaata caattccttt aagaaaattt acaccggttg aaatgaattt
     9181 gtttttttcc atcgtgtcta gaatgcgtga aaaaggggat aagacagttc gttttacgtt
     9241 tgaccaacta aaagatttaa gtaactacaa agcaactgca aatgtacgat ttgtagaaga
     9301 tttaaaagca acttatgaaa aaatcttatc tttacgcttt ggtcgtagaa gtgagtctgg
     9361 gctttcttac gatatgtttg taatgtttac agaatttaaa atcaacgggg acgtagaaaa
     9421 tccttacgta gatatacgaa tttacgaaaa agcactgcct ttgttaaata atttagacga
     9481 gtgggtacgc tattctttac aacaattcaa cgagctacaa agtggctatg caaagactat
     9541 gtttcgtctg ttaaagcaat acagaaccaa aggtttcgca tatttctcca aagaagactt
     9601 tcttgaatta ttagatattc ctaaaagtta taagcaacct gatatagata aacgagttat
     9661 aaaacctata cgtcaagaac taactgcaat cttcaaagga ctaacaatca aaaagaaata
     9721 cggaaaagga cggggaaaac ctgttattgg ctatcaattt acgttcaaac ctgagatcaa
     9781 aaaagcagac gactttaaca aacatgcacc aaccaaagaa gaccaaaata aagcattggc
     9841 tttagctgat aaacaaaaag aatacgaaga acccgctaca acaattaacg aaaaaatcaa
     9901 acagaagaaa cgagaacttg aactcctcca acaaatcaaa caactagaaa aagagaaaaa
     9961 agctcttgaa aaacaaacag agtccgcact ttcggaaaaa gagaaaaaaa gtttattatc
    10021 tgatttagat aatttattcc agtagcaacc taggaggcaa ccagtatgca accacaagaa
    10081 acctttacaa taaaggaatt gtcagatctt tttaagatga gtaggcaagc aatatcgaaa
    10141 catatccaaa aattagattc ttccatgata gctaaaaatg agcgtggtta taaggttgta
    10201 ttgcgttctg gagtattgca acttgctcgc aacttagaca atcaaaggtt gctagataag
    10261 ttgcataaac atgaacaaaa caacgataaa aatgcttata taccaaacaa aaacgtggtt
    10321 gcaaacaagt tgctagatca gttgcgtgaa gaaaatgaat ttttacgaga acaacttgcc
    10381 ctcaaagagc aaaataattc tcatatacaa aaactactcg accagcaaca acaactcacc
    10441 ctgcaagcca accaacaaat cgaaaaactt caggagcaac tgcagttgac ttatacggaa
    10501 gagtcttcag agaatagttc aacgactctt tctgaagcaa cagaaacaat ggacgaacaa
    10561 acaaagaatt ggaaaaaatg gtggcagcta tggaaatgaa gaaggaaata cttatgacaa
    10621 aaaaacactt gctattcggt tctatcctac taggaatatt aagctttagc ccaatgttat
    10681 tatcagtggc caacaaagga aataaaaaag gggaactaaa actcaatcct aattactaca
    10741 acagtcaaaa tgaggaagta tttgtagaaa aacttgataa ataagaaaag actgctcgta
    10801 cgagcagtct ttctgaaaga gattatccta ttattaacct tagaaaagaa taataaatga
    10861 atatgagata tgtaaaaagc ataacaaaca caaacaaaaa taacaagtat ttaaaaaatg
    10921 accgatcaat attcacaaag aattagaaag aaacaaccgc agaaaaccaa gccttaaaaa
    10981 gagcttttaa cctagaccaa taaaaattca caaaatcagc gcctggatcc cttggtacag
    11041 gcgttacttg gtagtataca cattatgtct actagctgtt taaataactg atcttataat
    11101 tttatttaaa cgattatgaa ttcaaaacta ctttttatga cgttaatcat tgttatataa
    11161 taaaatgtta tataataaaa ttactaagat tatttaatat aacaagcaat tttaaaaaaa
    11221 cgaaaaggaa aaacaatcat gaaaaagaaa gtattaaaac attgtgttat tctaggaata
    11281 ttaggaactt gtctagctgg catcggtaca ggaataaaag ttgatgcagc tacttactat
    11341 ggaaatggtc tttattgtaa caaagaaaaa tgttgggtag attggaatca agctaaagga
    11401 gaaattggaa aaattattgt taatggttgg gttaatcatg gtccatgggc acctagaagg
    11461 tagtggtagg gaggttatta taatggattt tactaaagaa gaaaaacttt taaatgcaat
    11521 tagtaaagta tacaatgaag caactataga tgactatcct gacttaaaag aaaagctctt
    11581 tctttattct aaagaaatca gtgagggaaa aagtgttggt gaagttagta tgaaattaag
    11641 tagttttctt ggaagatata ttttaaaaca taaatttgga ttacctaaat ctttaataga
    11701 attacaagaa attgttagta aggaatctca agtatataga ggatgggctt ctattggtat
    11761 ttggagttaa ttctttggaa taaaatgaaa atgggactga agaatcaatt ctgattcatc
    11821 agtcccataa attatagagc cgaaaaatta tcatatttat agatttatta atagtaaatg
    11881 gcataagaat ctttgaacaa cgaaaaaaat gtagctctac ctaattattg atttgcaaca
    11941 atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt tgctagtctt
    12001 ttttgtattg tggagagtgg ggg
//
LOCUS       DAFGZE010000087        11895 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.090, whole genome shotgun sequence.
ACCESSION   DAFGZE010000087 DAFGZE010000000
VERSION     DAFGZE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.090"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            587..1558
                     /locus_tag="LTU54_002504"
     CDS             587..1558
                     /locus_tag="LTU54_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBL8297168.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            complement(1636..1872)
                     /locus_tag="LTU54_002505"
     CDS             complement(1636..1872)
                     /locus_tag="LTU54_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8297169.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1841..1972)
                     /locus_tag="LTU54_002506"
     CDS             complement(1841..1972)
                     /locus_tag="LTU54_002506"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBL8297170.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            complement(2048..2431)
                     /locus_tag="LTU54_002507"
     CDS             complement(2048..2431)
                     /locus_tag="LTU54_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBL8297171.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            complement(2464..3249)
                     /locus_tag="LTU54_002508"
     CDS             complement(2464..3249)
                     /locus_tag="LTU54_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HBL8297172.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            3427..4527
                     /locus_tag="LTU54_002509"
     CDS             3427..4527
                     /locus_tag="LTU54_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBL8297173.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            4517..5341
                     /locus_tag="LTU54_002510"
     CDS             4517..5341
                     /locus_tag="LTU54_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBL8297174.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            5338..6153
                     /locus_tag="LTU54_002511"
     CDS             5338..6153
                     /locus_tag="LTU54_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL8297175.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            6150..6980
                     /locus_tag="LTU54_002512"
     CDS             6150..6980
                     /locus_tag="LTU54_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8297176.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            6987..8018
                     /locus_tag="LTU54_002513"
     CDS             6987..8018
                     /locus_tag="LTU54_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297177.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(8125..9398)
                     /locus_tag="LTU54_002514"
                     /pseudo
     CDS             complement(8125..9398)
                     /locus_tag="LTU54_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            9625..9810
                     /locus_tag="LTU54_002515"
     CDS             9625..9810
                     /locus_tag="LTU54_002515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297178.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            9934..10650
                     /locus_tag="LTU54_002516"
     CDS             9934..10650
                     /locus_tag="LTU54_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBL8297179.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(10552..11072)
                     /locus_tag="LTU54_002517"
                     /pseudo
     CDS             complement(10552..11072)
                     /locus_tag="LTU54_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            11360..>11895
                     /locus_tag="LTU54_002518"
     CDS             11360..>11895
                     /locus_tag="LTU54_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8297180.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKD"
BASE COUNT         3413 a         2198 c         2718 g         3566 t
ORIGIN      
        1 ataaaatgga ctttgtctac agtctggata acgtgatttt ttgctgtaat gacaaggttt
       61 tagctgtttc agataccatg tatccaaaaa tagtttattg agaaaatagg agagtatttg
      121 atactaacta gtaggaaaaa atgagataaa aagtaaatgg aatcatttaa ggtaatctga
      181 aataaaaaat atacagccct gtggtacttt acctacaagg ctgtatatat tttttactct
      241 cagcgttttt gactccgtgt ctgactccgt tttattgatt ttgaatgtta ttcaatgacc
      301 atttaacagg tcaaatttga tgtttttgat tccgtatgac gccgtttgat atctagtgat
      361 atagtgattt gagtctacat cgaataaggg aataaaccaa taaaaaatga tttagaatat
      421 taaaataaca aaaaagagga ctacctgtat tagtcctctt ttttgaccag attttgacca
      481 gtctatttta catcctagta tatttatatg agctaatttt atgattaaat ggtaagtttt
      541 tccttgtgtg ttttagttta aactagtgtg taattcaaaa atctatatgg aaggaacagt
      601 attaagaata gaacagggtt ctctacatga tggtgctggt ttacggaccg ttgtttattt
      661 aaagggctgc ccattacgct gtgcctggtg ttcgattccc gaatcacaaa gcaagcagat
      721 tgaaaaagga tttggccaga ccatgaccgc tgaagaggta atggatgaga ttgagaagga
      781 tgctgttttt tactttcact cggatggcgg agtgaccatt agcggaggcg aggcactggt
      841 tcaggcggat tttgctaaag aaattttgca aaaatcgaaa tacattggaa tcaataccgt
      901 actggaaacc agcttttgtg gtgcttataa tgaaatccaa aaggtagctc cctatgtgga
      961 tacattgttt gtggatgtaa aaatgtttac cagcaagctc cacaaacaat ggaccggctt
     1021 agataaccaa caaattttga agaatattcg tcgcttttta attgagtatc ctaactgcga
     1081 agtgcgaatt cgagttcctg tggttccagg tatcaatatg aacctgaccg agctgctgac
     1141 gattgcttgt tttgtggcag atcttgatcg gttcgtaccg ttagaattac taccgtacca
     1201 ctgctatggt atgcacggtt accaagcatt gggactggag tatccgttgg cagacacgcc
     1261 agcgccttca cctaaagaaa tgtttgcatt ggcagatcaa ctggctcgca cggttcctca
     1321 tttgctggtc acagctttaa ccaaaacatt tatttattta ttgaatccaa gctttatctt
     1381 atggataaat gaaagacact cactagctgc agccagctca agaatagagc ttttgctgaa
     1441 gtcagtgagt gtctttttac gatgtttttt cgaagttaat ttgtcgaatg aacactggct
     1501 caatcggtac atcttcactg tttgtcggct gagctgcgat cgctgctacg atttctaacc
     1561 cttggtatac ctgtccaaaa acagtataac gaccatccag ctccggtagt cctccaagct
     1621 ccaagtatgc ttgctttaca ttagcaggtc agcctgcgcc tttgtgtttt gcacaataaa
     1681 aaactggttg ccgttggtgt gggggccgtg gtcccctaag gccaaggcgc cattaaaatg
     1741 ataaagtccg cgatgcactt catcagcgaa aaagccctca tagctggaaa cagcttcatc
     1801 accagaacca tccaaagcgc caccttgaat aacaaattcc ttaatgacac gagccgaaag
     1861 gtgtgttgtc ataaacgcct tgctcagcta gggagcgcca gttggctaca gccaggggag
     1921 cttggtcagg aaataagcgg agacgaattt caccagcatc ggtgaccagc acacaaactt
     1981 cttcctgctg cggtttttct aattgaaaga gaggaaggtt tgcaactgaa acgccttgat
     2041 cctgccatca aatatcgaaa atccgccact cgaccttcgc ctgatcacca aggcgagcca
     2101 ttcccaataa aacggtatag gcggtttcat ccgcttgaaa ggtcaattgc agctgtcctc
     2161 gtaaatcgtt gtagtcatct ggggcggcta cttgataaaa aagatgaaac tgcgcatgca
     2221 aatattccaa aggctgatct ttaaaggctg caaaataagc ctgaactttt tcccgctgca
     2281 agctgtcagc caccaaaaaa ttttccagtt gttgttttgg aacggctgct aacaaggatc
     2341 ggcaaaccgc ctcaattttc aacgcatccc aatgtcgcgc aattaaataa tggatgggtg
     2401 cctcttgatt gtagtcccac aggttgctca tttttttcac cactttcttc tattatatag
     2461 gaatcacaaa atcggcagcg gcgtcaggcg gcgttctttt ccttgaatga ctagcaactt
     2521 ttctacctga cagtctgcca acagctgact cattttttta aagcccagct gataatcttc
     2581 ggcaacatga gcatccgagc ctaaggtaaa gagctttccg ccgacacttt gatacaaagg
     2641 aacggcataa cggtaaaggt cttcgttgcc gtatttcaag aagctcttgg catttaactc
     2701 catcgccaat tctcgttcaa caaccttttt gaaaatcgtt agcagctgag cctcaaaatg
     2761 ctccgcgaat tcttccgctg tgaaggtgaa gcggcgtaat ccataatcaa agtgagtcaa
     2821 aatatctcct tctggaaaat ccgtcaagac ctgcaacatc tgatcaaaat attccttggt
     2881 agtttgaatg ggatctttgg ttaaaaccac atcgtccata taatcaaagc ggccattttg
     2941 atgaatactt accagcttta aatcataagg atgctgagcg aggtaatcca cgatccgctc
     3001 ttcttgtcct ggaacaacgc caatctcgat acctcgcaga aatttggtgg aataggtttc
     3061 ttttaaagag tccagtttgt ttatataggc tggataatcc ggaatgtcat cttttccggt
     3121 agatggattg tttaaatcaa agtgatccgt agaaacgaaa aactccggct gataggctaa
     3181 atagttttca aatttttcct ctgaatcaaa ggaaaaatac gtgtggagat gctgatcata
     3241 atactgcata gggagcctcc ttttttctac tatcataccg caaaagcttt agaaggttaa
     3301 cgggaattcg tcgaaattta caaaccatat acaaccttta cattgttcat gaaacctttt
     3361 caagaaactg attctctcat tacaaagctt tgttagaatg gaattatcga aaggggagaa
     3421 gaatgaatga aaatgaatga aaaaattcgt aacatgacta tcggtttatt ttgtgcagca
     3481 acagtaagta tcctgctgac aggctgcggc tcaggtgaag caacgaagca atcggctgcg
     3541 gcagacgact ctttagatac gattatagaa aaagccaagg aagaaggaga gattgccagc
     3601 ttgggaatgc cggatacttg ggcaaattgg gtaggcactt ggaacgacct tgaaaaagaa
     3661 tacggcttaa gtcacgtgga tacggatatg tccagtgcag aagaattggc taagtttgaa
     3721 tcagaaggaa aaaatggaac cgccgatatt ggtgatgtcg gtatcagctt cggtccgttg
     3781 gctgaaagca aagagttaac cttaccttat aagaccaact attgggagga aattccggat
     3841 tgggcaaagg atgatgatgg ggattggttg ttaagctata cgggaaccat ttcctttatc
     3901 accgataaag agaatgtgaa gcaagcaccg actagctgga aggagctgca agagggtgac
     3961 tatcaagtta gcgtggggga tgtgacgatt gccaaccaag cacaatttgc tgtattggct
     4021 gcggcgattg ccaacggagg tgacgaaaag gatattcagc caggtctgaa ttactacaaa
     4081 aaaatggcag aaaaaggccg tttgtccacc gttgatgcca gcgtagcaaa tcttgaaaaa
     4141 ggtgaaatcg atgtggcagt tgtttgggac ttcaatggac taaattatcg tgaccaaatt
     4201 gactccgagc gttttgaggt aactgtgcca acagacggct ccatcattag cgggtataca
     4261 accttaatca ataaaaatgc ccctcatccc aatgcggcaa agctagcacg ggaatatatt
     4321 ttatctgatg aagggcaaat caatttggca aagggctatg cgcgacctat tcgcaaggta
     4381 gagctgccta aagaagtagc tgaaaaatta ctgccggagg atgcttatga agcggcggtg
     4441 ccagttgaag acaatgaagc ttggaacaag acctctgtag agcttccaga gctttggcag
     4501 gcggaggtac tgacctatgc aaagtagact tgacaaactg attgtagtta tactagacgg
     4561 ctgtcgctac gacacagccg tcgcccagct tgggttcatg aatcatttag ttgagcacca
     4621 gcaagcaagc ttattgaagg taaaaagtga gctgcccagc aattcacgtc cgttatacga
     4681 agtactgatg accggcgttc cagcctatga aaatggcatc tatagtaatt accatgctca
     4741 gcgatccaaa gagtggtcgc tgtttgaatt agttaaggaa gctggcggaa aaacaggagc
     4801 cgccgcttac cactggtaca gcgagttgta caatcaagcc ccctataatc gtttggttga
     4861 tcgcatccag catcaggagg agcgactgat tcagcatggc atcttttata gtgaggacac
     4921 ttaccctgat tcgcatttat ttgcggatgc ccattattta gtgaatcagt atcagccaga
     4981 ctttttggtc gtacattcga tgaatattga tgatgtcggt cacaaattta ccgccgattc
     5041 tcatgaatat gcagcagcgg ttaatcaggc ggacagatta ttggctattt accttcctga
     5101 atggctgcaa aaaggttatc aagtcgtggt gactgcggat cacgggatgg atgcgcacgg
     5161 actgcatggc ggcagcttgg ctgcacatcg tgaagttcct ttttatttga tttcaaataa
     5221 acgccaaaag ctgaaggaac aaactgtacc acagctgctg gcggctccat tgttttgctg
     5281 gttattagga attgcaccgt cagagaaaat gcaggacatt caagaattga tgaggggatg
     5341 aaagagatga aaaaacgctt ggttctggtt cctgcctcct ttatcttctg tgcattttta
     5401 ctgctgccgc tgctttttat ggttctgtgc agcttgcaca gtgatttaac taaccagtgg
     5461 acattggcca attacagtga tattttcagc aacaaatatt acacacaggc ctttgttaac
     5521 agcgtactga ttgctgtcag ctccagtgtg attgggttaa tcgggacgtt tattttatgt
     5581 ttatgcttaa tgaatctatc gactaaattg caggagaaaa tcacctttgt ttccaatttg
     5641 gcagctaatt ttgctgggat tccgctggca ttctctttta ttattttgtt ggggaatgta
     5701 ggtgtgctga aaatgatttt gccatttcta gcggacttca atttgtattc ttggtgggga
     5761 ttgaccatca cgtataccta tttccaaatt ccgttagggg tcttgttttt gtatcccagc
     5821 atgaaggaag tgaaaaagga atggctggag tccgcacaat tgctgggcgc ttcatcagcg
     5881 tatgcttggt tcaaggtggc tttgcctttt ttgcgaccat cgattgccag cacctttgtc
     5941 atcttatttg ctaatggcat ggggacctat gaaacagcgt atgccctgac cagcagtaat
     6001 gtaaatcttt taacgattcg aattgcggct ttagtgtcgg gagatgtgtt cgccaagcct
     6061 aatttaggca gtgcgttggc agtcgcttta ggtgtcctgc tggtaacggc aatgttgatt
     6121 attcaaaaga aaggcaaggt ggtgcagacg tgaaaaagtt aataaaaccg attttgttct
     6181 gctttgtcct gtattacttg ctgccgatta ttggaacgat tttgtatgcc agctccacta
     6241 aatggtcaaa atcgctttta cctagtgatt ttaccttgca atggttccaa cagctgctga
     6301 cggacaggga atttattgct gcggtgggtc gctcactttt attagcaggt gtggtcttgg
     6361 tgacgattct gttgttgatg attcccacca ttatttggat tcatttgtat ttccctcgtt
     6421 tgaatcgttg gctggaaaag ctgctcctgc tgccgtatgc tttaccagga gtgattttgg
     6481 taacggcttt gcttcgaacg tatgctgaaa ctggtatccc aatgtttgta gtgttggtag
     6541 gtgcattgtt tattacggcg ttgccgattg tctatcttag tcttaacaac cagatgcgct
     6601 taattaatct gaaggaatta gtggatgcag cagaaacctt gggcgccccg atgtctacga
     6661 ttattatcca ggtgctgttt ccaaacatcc gtatcggtgt gaccctagtt tctctgatga
     6721 ttttttcttc ggtttttggg gagtacatgc tgacgaacct gttgattggc ggtcgatttc
     6781 agacggtacg catctatatg ctgcggcgga tgaacgaaaa cggccatttg gctagcgctg
     6841 tgatggtttt gtatttgctg tttatggtgg gaattgcttt aactaccttt atgctgactt
     6901 atcgacaaaa gcaacagctg tcccttaagc agacgaggga agagaagcag tcgaaaaaga
     6961 agcccttaaa ggagttgtga cggaaaatgt ttttagaaat acatgatctc aatctgtttt
     7021 ttgatgaaaa acagatttta gctattgatg aaattcagat tgaaaagggt gagctggtta
     7081 ctttactagg tcccagcggg tgcggaaagt ctacgttgct gcgctgtatc acgggattgg
     7141 aacaaccaaa gagcggtgag attgtgctgg acaatgagaa tattgccgat aagcctacga
     7201 aggatcgcaa tatcggcttt gtttttcaac agtatgcatt gtttccaact atgactgttt
     7261 ttgaaaatgt agcctttggt ttgaaggtaa agaagctgag tcctgaggtg attcaggaaa
     7321 aggtttttga gatgctatca ttggtggata tgacggagca ggcagataaa aatgtccagt
     7381 ttctttctgg cggtcaacga caacgggtgg cactggctcg ctcattggtc actgaaccga
     7441 aagtcctgct gctggatgag ccattaagtg ctttggatgc ccgaattaga aaacagctgc
     7501 agcgggattt gcgcgctatc cagcaatcgc tagggatgac gatgattttt gtgactcatg
     7561 accaagaaga ggcgatgcga atcagtgatc ggatttttgt gatggaagct gggcgggtgg
     7621 ctcaggtttc cacctcgaag gaattgtatc agaatcctca gagccgattt gttgcggaat
     7681 ttatcggcaa ctataatcgt ttcgaatggt atgagctgaa tcagtatacc cacgagtttt
     7741 ccgctaaggg caatcgctgc atttattatt taagaccgga gctgattcaa tttgaaccgg
     7801 ttcctcaggg ggttaagatt ccggttctat ggaaggaatc atttatttta ggcaacattc
     7861 aacggtatgt ttttcaaacg ctggagggca aagaaatttt agtggatcgg ttgaacgatc
     7921 gggaagagaa aattagcgat gccttataca ttcagctgaa agatattcta gcaatccctg
     7981 tggaaattga tactcagcat tttgcacaac aaagctagtg tatagtcggc aaacatttct
     8041 ggctctttgt caaataggac tgatagagtg atataagaac atctaaggta gaattgattt
     8101 tctgctttag atgttttttt atgtttaata agtgatcaga tgattgatta agaaaattct
     8161 ggttctcatc ctaacccgta agctattcgt ttcaatgctt tgatgtgggt atgcagattt
     8221 tctattttgc catttgagta aggcaactct aaggctaaac gaatgccgga ttcatagttt
     8281 ataaacgctt tttttatatg atgcagttct cttggaaccg acttgggcat tgtttttaca
     8341 agctgaaaga agatgtccgc ttctttgtca taaaaagctt gaagaatttc ttgatagtaa
     8401 gcataagcta ttcgtagttc atcagaagta gaaagtagac gttctacgac ttcactttcg
     8461 gttaaatagg cccaaccaaa attacgccat tttgtccatt tatcataaac aagcgtgtcc
     8521 tgtcgtttga tcagaagtcg agataaagcc ttcacttgtc gatagtgttt ggcttgtatt
     8581 ggatcgtttc ggtcgaagct tttcatgact catagtcaaa tatccttaaa actgcgaatg
     8641 aggtgcttga cgatgtgaaa gcgatccaca acgagtttgg catgaggaaa gcactctttt
     8701 atcagcaaag cataagaggc gttcatatct gttacgagta tttttacgtt taaacgtgca
     8761 cgtcgttcaa attgcataaa ataagaaatg agatctttct tcttacgtga aggaagaata
     8821 tccagtaatt tcccagtatc gccatccata caagaaaaag aatagggacc gacttgattt
     8881 ttcaatgaac gaaattcatc taccaatgaa cagcgaggca accaatttat tttcacgttt
     8941 gtcatgggga gcagagaagc gagtgttcgc gaaacggttg accaagaaac gccgcaatgt
     9001 ttagaaatcg tcgtcattgc ttgtttttca gtcaattcct ccaatatttt ataatgtact
     9061 tgtttggcga taaaacagtt tttttcaaca atgtaggtat tcgccgtaaa ataggtgtga
     9121 cagccacgac agaggtaccg ttgctttgcc aaagcaagat aagtccgttg attcgcacac
     9181 ttattgagaa ggaccagcga ttttttcttt ccgttcttaa cgatcagccc atgttgattc
     9241 acctgtttac actgaggaca gggctgaatc gttttaatga gcgtggcaga aacaacgata
     9301 ctgttgattg aatgtatttt tttcttctcg acatgagtga tttgaatgtt tccatctttg
     9361 attcctagta atagtttgat aagatgaaga tcaggcatac agaacgctcc tttgattgtg
     9421 ttgtggtgat tcaattctaa cggagcttct gtatgttttg tttttttatg ctcaaaagga
     9481 ctgatagaat gatttactat cagtcctaga aattatagcg ccacatttct ttcacctttg
     9541 acttttcaga cggcagtttt tatgctgata ctgtagagag agcaggacac agcttttgct
     9601 tgttttgggt aagggggaaa gaagatgacc aaagcactta tttttgattt tgatgggacg
     9661 atgattgaca gtgaatggat gttttacgag agtattcatc actatttact gaagcctatt
     9721 tactggagca gttcggctat aaaattcctt ttgcagacta tcaacaaggt gttgggacgg
     9781 cagcagaact gtccttttat gaagggataa gtcaagcggt tgggagacca gtggatgctg
     9841 aacattttga gcagcggatc agcagcactg gaaaatcgga gtagtctcaa ttctgaactg
     9901 cgggagctag actatttttt tgaagcacat ccgatgatta aggaaaaaat tgattccttt
     9961 gtgacgatcg atgatcttca ggtcggcaaa ccagatccag aaggttattt gttgtgcttg
    10021 aaacggctga gggttgaaca ggctgttgta gttgaggata gtccagtcgg tgcccaagca
    10081 gcaccgcata tgacagttga tgtacaagcg ttggatgcag attttttcgc ctttagcggg
    10141 cataaaatgt gcgggccgac aggaatcggt gtattatatg gcaaacgtca attgttagaa
    10201 caaatggagc cagtcgaatt cggcggtgaa atgatagatt ttgtcaatct ttacgatagt
    10261 tcatggaaag agctgccatg gaaatttgaa gctggtactc cgaacattgc aggagcaatc
    10321 gattacttga atgagattgg tatggagaat attcatcgtt atgagcaaga actggttgcc
    10381 tacgttttac ctaaattaca agcgatcggc ggtatcacca tttacggacc gcaagatcct
    10441 gaacatcata ctggcgtggt tgcttttaat ttggatggca tccatccccg tgatgtggcg
    10501 actgctctgg atatggaagg tgtcgctgta cgttcaggac accattgtgc acaacggttc
    10561 atcgacgatt tctgtttgac taattggtcg tttaaacagt cgatgataat gccgtttgag
    10621 cggctccaat gtcgtggaat ctttgagtaa gagtttccaa tatcgcttga gccgacggta
    10681 cttcttctga ttttcagatt acgtgattga ttttcgtaca gttctatgag aatcgcctgc
    10741 ttcagttcct ttgttagttg atgatgttca tcaactaagt ccgttttcgc agaaaaggtc
    10801 tgcccacaat catagcatag aaaacgggta cgttttaatt ctaaaatagt caacttttct
    10861 ttgactttca acatctgagt acgtgtcgta taggaaccgt tgcggataat acgaatcgat
    10921 ccacagtgtt cacaatggga aggtgagtca tccaatctcc ctttaataaa ctgcgcttga
    10981 acgcctttat attctttttc aatcagccat tcttcatcaa aaaataaatg tttatctgtt
    11041 aatccgagta attttctagt atgattatcc atagagcgac tcctttggta gggtgatgtt
    11101 ttggtcgact aaattttacc aaaagtgagt ctctttttga gttgaaacga agaaaaagtg
    11161 ttagtagaaa atcgccttcg attttccaac aacacaaaat attatagaag ccttttttct
    11221 aaaggatttc aaggtgtctg tttgcattta ttaaaaaaaa gaaaaagtat tggcaacaga
    11281 aggcgtcacc aacactagat attatagagc cataatatgc tgtataatat ctagtgtata
    11341 ggcttaagtg gaggtatata tgaaaatatt aactgttgaa gatgataata tgataagaga
    11401 gggaataagt gaatatcttt cagaatttgg atatactgtt attcaagcta aagatggaag
    11461 agaagccttg tcaaaattta atagcgatat aaatttggtt atcttagaca ttcagatacc
    11521 ttttataaat ggtttagaag tgttgaaaga aattagaaag aaaagcaatt taccaattct
    11581 aatcttgact gcatttagtg atgaagaata taaaattgat gcatttacta atttagtaga
    11641 cggatatgta gaaaagccat tttcattgcc ggtcttaaaa gctagaatag attctttaat
    11701 taagaagaat tatggacatt tagagaagtt tgaatataag gatctatcgg ttaattttaa
    11761 tagctataca gctaaaataa atggtgaaga aatagatgta aatgcaaaag aactagaagt
    11821 attaaaatgt ttattggata ataatggaca agtgttaaca agaatgcaaa ttattgatta
    11881 tgtatggaaa gatag
//
LOCUS       DAFGZE010000088        11643 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.091, whole genome shotgun sequence.
ACCESSION   DAFGZE010000088 DAFGZE010000000
VERSION     DAFGZE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.091"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            316..675
                     /locus_tag="LTU54_002519"
                     /pseudo
     CDS             316..675
                     /locus_tag="LTU54_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="LTU54_002520"
     CDS             complement(970..2103)
                     /locus_tag="LTU54_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8297181.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="LTU54_002521"
     CDS             complement(2094..2762)
                     /locus_tag="LTU54_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8297182.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="LTU54_002522"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="LTU54_002522"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HBL8297183.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="LTU54_002523"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="LTU54_002523"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HBL8297184.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="LTU54_002524"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="LTU54_002524"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HBL8297185.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="LTU54_002525"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="LTU54_002525"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HBL8297186.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="LTU54_002526"
     CDS             complement(6138..6911)
                     /locus_tag="LTU54_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8297187.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="LTU54_002527"
     CDS             7076..7555
                     /locus_tag="LTU54_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8297188.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="LTU54_002528"
     CDS             7612..7914
                     /locus_tag="LTU54_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBL8297189.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="LTU54_002529"
     CDS             7936..9393
                     /locus_tag="LTU54_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBL8297190.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="LTU54_002530"
     CDS             complement(9986..10738)
                     /locus_tag="LTU54_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8297191.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="LTU54_002531"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="LTU54_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(11199..>11643)
                     /locus_tag="LTU54_002532"
     CDS             complement(11199..>11643)
                     /locus_tag="LTU54_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8297192.1"
                     /translation="HKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHL
                     FKSMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDF
                     RDMDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
BASE COUNT         3343 a         2510 c         1994 g         3796 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatccat gtccctaaag
    11341 tccattgttt taggtaagcc atctcgtcta agcaagccgt ttgagtgctc atttagccct
    11401 ctttgcgaag gtgttcctgg atctgcgaaa aagatggcaa tatcgttcga gttacttata
    11461 gatcgccagt tggaaaattc ttttccacaa tcaaatgtca tggatttaaa caagtgttta
    11521 ggcatggatt tgaaccaatc atttaacctg ttttcgatat cttcggctcg tctgcctgtc
    11581 ggtttcaaag taataataac cttggataaa cactcaacca atgtaataac agcgctcttg
    11641 tgt
//
LOCUS       DAFGZE010000089        11587 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.092, whole genome shotgun sequence.
ACCESSION   DAFGZE010000089 DAFGZE010000000
VERSION     DAFGZE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.092"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            236..1876
                     /locus_tag="LTU54_002533"
     CDS             236..1876
                     /locus_tag="LTU54_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBL8297193.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2030..3352
                     /locus_tag="LTU54_002534"
     CDS             2030..3352
                     /locus_tag="LTU54_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HBL8297194.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3414..3845)
                     /locus_tag="LTU54_002535"
     CDS             complement(3414..3845)
                     /locus_tag="LTU54_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8297195.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(3909..4745)
                     /locus_tag="LTU54_002536"
     CDS             complement(3909..4745)
                     /locus_tag="LTU54_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBL8297196.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(4935..5237)
                     /locus_tag="LTU54_002537"
     CDS             complement(4935..5237)
                     /locus_tag="LTU54_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBL8297197.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="LTU54_002538"
     CDS             complement(5263..5694)
                     /gene="ruvX"
                     /locus_tag="LTU54_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBL8297198.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(5691..5957)
                     /locus_tag="LTU54_002539"
     CDS             complement(5691..5957)
                     /locus_tag="LTU54_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBL8297199.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(6211..6936)
                     /locus_tag="LTU54_002540"
     CDS             complement(6211..6936)
                     /locus_tag="LTU54_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HBL8297200.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(7302..7372)
                     /locus_tag="LTU54_002541"
     tRNA            complement(7302..7372)
                     /locus_tag="LTU54_002541"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7338..7340),aa:Gly,seq:ccc)
     gene            7489..8241
                     /locus_tag="LTU54_002542"
     CDS             7489..8241
                     /locus_tag="LTU54_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL8297201.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            8257..8736
                     /locus_tag="LTU54_002543"
     CDS             8257..8736
                     /locus_tag="LTU54_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBL8297202.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(8987..10567)
                     /locus_tag="LTU54_002544"
     CDS             complement(8987..10567)
                     /locus_tag="LTU54_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBL8297203.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(10560..11303)
                     /locus_tag="LTU54_002545"
     CDS             complement(10560..11303)
                     /locus_tag="LTU54_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297204.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(11479..>11587)
                     /locus_tag="LTU54_002546"
     CDS             complement(11479..>11587)
                     /locus_tag="LTU54_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBL8297205.1"
                     /translation="NEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
BASE COUNT         3520 a         2313 c         2057 g         3697 t
ORIGIN      
        1 tctatttatc tatctaacat atcctttatt cttctacttt tctttcttta gcatataaga
       61 gatacgcata gtaatcaagc aaggattgtt gatataaaaa gaacaaatac cgctattttg
      121 atttcttttg actctattac tcatataaat aaaatttatt atacctgctc tttttgacta
      181 tatcctcatc tgactcctat acttcgtttg tattaaaact taggaggaat cattcatgct
      241 tacagggcta aacttattaa ataatccttt tctaaataaa ggaacagctt ttacaaaaga
      301 agaacgggca aaatacggaa tcactggtat gcttccaagt actgtccaaa cattggaaca
      361 acaatctgtc caagcttacg gtcaatactt aagcaaacag accgatttgg aaaaacgtat
      421 cttcttaatg aatctattca atacgaaccg gacattgttt tataaattga tgggcgaaca
      481 tttagttgaa ttcatgcctg tcgtttatga tcctgtcgtt gcggattcaa tcgaacaata
      541 caacgaaatt ttcttgaaac cacaagatgc tgtatttctt tcaatcgacg aaccagaaaa
      601 tatcaaagca agtttgaaaa acgcagcaga tggacgcgac attcgtttga tcgttgtgac
      661 agatgcagaa ggaatcttag gtatgggtga ctggggtgtg aacggtgttg acatcgctat
      721 cggtaaattg atggtctaca ctgccgcggc tggtatcaat cctgcacaag tcttgcctgt
      781 atcaatcgac gctggtacta ataacgaaga attattgaat aatccattat acttagggaa
      841 tcgtcatgct cgtgtagaag gcgaaactta ctacgaattc attgatcaat tcgttcaatc
      901 agctactgaa ttattcccag aattgttatt acattgggaa gattttggac gaggaaatgc
      961 tgcaacgatc ttagaaaaat acgaagataa aatcactact ttcaatgatg atatccaagg
     1021 cacaggaatc gttgtactag ctggtgtatt aggtggattg aatatatcag gtgagcaatt
     1081 gaaagatcaa acgatcttga catttggtgc tggtactgct ggtgtaggta tcgcaaacat
     1141 ccttttagat gaaatgatcc gtcaaggtgt acctgaagaa gaagcacgcc aacatttcta
     1201 tcaagtagat aaacagggtt tattgtttga agatacaaaa ggattgactc ctggtcaaat
     1261 cccattcgct cgtaaacgct cagaattcac taatgctgat gaattgacaa acttagaagc
     1321 agttgtaaaa gcaatccatc caacgatcat gatcggtact tctacccaac ctggagcatt
     1381 cactgaagaa atcgtgaaag aaatggcggc tcatacccca cgtccaatca tcatgccttt
     1441 gtctaatcca acaaaattgg cagaagcaaa agcaaaagat ttgattgaat ggacggacgg
     1501 aaaagcttta gttggaacag gtatccctgc tgctgatgtg gaatataacg gtgtaactta
     1561 ccaaatcggt caagcaaata atgcgttgat gtacccaggt ttagggcttg gtttgattgc
     1621 ttcaacagct acacgagtaa atgctgaaat catttctcaa gctagtcgag cattaggcgg
     1681 aatcgttgat gtaacaaaac caggagcagc tatcttgcct ccagtagcaa aaatcactga
     1741 attctctcaa acgattgctg aaactgtcgc aaaatcagtt gttgcacaaa aattgaatcg
     1801 tgaagaaatc acggatatca aagaagctgt tgaatctgct aaatgggttc ctgaatataa
     1861 atctttagaa gattaagatt gttatagtaa aagaacatac agaaactttg tatgacgtct
     1921 aaaaagaaaa aagcgactgc ggcaaaacag cagccagcca actactgctc ttttgccgct
     1981 tctctttggc ttttttcata cgatacagaa taagaaagga tctgtaaaaa tggcacaaaa
     2041 aacaacaacc gcaactgcag ttgaggaaaa gaaaaaagaa gggttttggt caacaaaaat
     2101 taccggcgta agtttgcccc tttatttgat catggtcctt gtcctgatca ttgcaatggc
     2161 tttaggaaaa ttaccgacga atatgattgg cccaatcgct gttttggtca ttttcggtaa
     2221 tttatttcat tttatcggaa caaaaatccc tatagtcaaa agttatttag gtggtggctc
     2281 cgtttttgcg atctttgcct ctgccgcgat tgctacattt gggatcttac ctgagtatgt
     2341 agtaaaatcc acagaaaact ttgtcagcaa tatgggcttt ttagactttt atattgctgc
     2401 cttgattact ggtagcatcc ttggtatgaa ccgtaatcta ttgatgaaag cttctgttcg
     2461 ttttattccc gttgcactta tttcaatggt cgtctccttt tttgccgtag gactcgtagg
     2521 tatgcttatc ggaaatggtt ttgcaaattc cgttttatat atctcattgc ccgcaatggc
     2581 tggtggtgtt ggagccggag ctgtaccttt atcaacgatt tatgcaaatg ttttaggtac
     2641 ggacgcttct tctatcattt cccgcctgat tcctgcgaca gcaatgacaa atgtcctagc
     2701 tatcattggt gcagcgttag ttgctcgtgc gggacaatca atgccaaaat tcaacggtaa
     2761 tggtaaatta atggaaaaag gcgaccttgg cgatggaaaa cctagaaatg taaaaccttc
     2821 cattccacaa ttaggtgtcg gtttgatggt atcattgact ttcttcatct taggacaaat
     2881 ctgtaactat tttgttccta aagtccatgc ttacgcattc atgatcatta tcgttgttat
     2941 ctgtaaaatc gcaaacctat taccagaata ttacgaagat gcagctatca tgttcaacaa
     3001 cttgattgtc aaaaacttga cagcagctgt tcttgccggt atcggtattg cgttattgaa
     3061 tctaaatgta ttggcttctg ctttgacatg gcaatttgtt gtcttatgtt tgacaagtgt
     3121 tatcgcgatt tcagtagttt caggatttgt cggtcgtttg tttggtttat atccaatcga
     3181 atcaactatt actgctggct tatgcaacaa ctcgatgggt ggtacaggaa atgtagctgt
     3241 cttatctgca gctaatcgga tggaacttat tgcttttgct caaatgggca atcggcttgg
     3301 tggtgccatc gttttgatca tctcaggttt cttgatgcaa ttactttctt agtcaaaaaa
     3361 aatacctgat catcaagtat cggcttgatg accaggtatt ttttgataaa aatttacagg
     3421 attttcaaaa atcttttttg cattttatcg gaatgataac cgatgcgttc ataaaatcgg
     3481 tgtgcttcta ctctgtattc tgcagaatta agtcttattg ctgatattcc tcgtcttttc
     3541 gcttcttcta cgccagtcat caaggccttt ccgattcctc ttttttcagc atgtttagac
     3601 acagcaagtg ccataatatt gaacatcgga tcgctgaaaa tcgattcata ggactccgct
     3661 tgtacatagc cagctgcttt tttagaagta ggatcttcat agaccaagaa aaaatttttt
     3721 tctggctcct tcaacagttt cttcatttgt ctcaccgtaa cggttaatgg tacatcatat
     3781 cctaattgtt ccgcattgat ttctcgtagt gcttctgcat ctcctaaacg catttcccgc
     3841 aacatccaaa tcaccagcca atttttattt ttttaaatga atttttttat cacagtatac
     3901 tccttttttc acttaaaaaa agacttttca gtgattttga cttgcttgaa tccagatcct
     3961 tctaatgttt gcaagatgac gaaagcattg taaagattga tcaaaagaac cacgatgaca
     4021 atcgcttgaa aaataaaggt cacttttttt ggagacaaaa ccctgctaaa tcttgctccc
     4081 cacaaacctc caataacagc agcggggata acataaaaca acatagataa atcaaagcgc
     4141 ataaagctac ttgttaaagc aatcgttacc aattttgcca gttgagaaaa gaaaatcgta
     4201 gatagagaat atagtgttgc ttcttttatt ggcaatgcaa acatcagcat caacaaagaa
     4261 acattgactg gtccaccacc aatacctaaa aaactagcta aaaagcccag aaccccccca
     4321 catataaggt accaagtcca tgacgtcaaa tgaaattttg gctgatgatg cttggtgtag
     4381 aagaacgcaa aaaccaaggt caataccgtc aaaaaaattt ggatcagctg cacatgcttc
     4441 tcgttttcaa ataactgtaa aaaaacttcg aatacaatgt tgccgactac tcctccaaca
     4501 acagctccac tagataccca accgacaatc tgccaattca gtgatttccc gcttgatact
     4561 tgtcgcacag tcgaaacaat ggacatcgtg aataccgcaa ctgttgagta aaaagaaatc
     4621 cctgcaacag aatgggcgcc gataagatca agaatcggtt tgatcaacac gcctccaccc
     4681 atcccggaaa tagcgccaac tgtgttcgcc agaacgatca ccacaaaata aatcagacca
     4741 atcatcttgt atttcctttc tcttgtttat atcttttagc gattgttact cttttctttt
     4801 attatacagt aaaagaaaca aacgaggctt cgaaagcttt tctacttttt aaggaaattt
     4861 agtgctaaac gaaaaaaccg ggctctactt ttcagagagt ccagcttttc ttacacctta
     4921 tcgattcttt tgttctattc ttcttccgcc atgaatgtat tgaatacttc ttcgatcata
     4981 tcccattctg cgtctgtttc gatttgttct aactcgcctt ctgttccatc ttcgttttcg
     5041 atgtatgcat aagcttgtaa ttctacatct tcatcttctg aaacaccagc tggatataaa
     5101 agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg ttagcaagat ttcgtataat
     5161 gtttcatttc cttggtcatc caccaatgtg atgtgttcgt gtccttcgtg ttcgtgggaa
     5221 tggttatgtt ctgtcataag attacctctt ttctttttat tattaaaata attgcttacc
     5281 tgagccgtct aaataatttt gtaaaatcat cacagctgct aatttatcga tgactttttt
     5341 tcgtttcgct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     5401 tagtcgttca tcttgataat caaccggcaa ggagaatagt tctttgatct tctctccata
     5461 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atattcttag gtaatccaac
     5521 tacaaaacgg tctacttcgt actgcgccac taattccttc aaccgatcaa aaccaaactc
     5581 ttcttctgcc tcattgattc gaatgatttc tactccttga gctgtccagc caaagggatc
     5641 gctcacagca acgcctactg tcttagaacc gacatccaag cccattactc tcataaattc
     5701 actccatgat ttgagaggta gtctttgaca attacttcca tgatttcatc gcgttcgtga
     5761 cggcgaatca aatttcgtgc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     5821 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     5881 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     5941 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     6001 tacaccctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttctgaa
     6061 taaattaaga aacacgattt gtctgatttt tccttttttc tgtggctttt tcaaaagccc
     6121 tctttccata aagtatacgc tatatttctt tgaaagtatg tttttacaca attttttcat
     6181 aaagttcata aaaaattcga attgctgcta ctaatttttc gttttcgttg cttgccctgt
     6241 ctcataattg atggtgtagc catcttgaac attgtagata aagacattga attcaatttt
     6301 tttgtcttca atgctttctg cttccaactg cacacctctt gctaccagtt catttccctc
     6361 gaaatgagga gtgacacgat agcgtacatg atgatttgtt tccttgatat atgccgcaat
     6421 atcattttcg tgggcaagca tgtacggagc attcaacgaa cgtgtccctg tcattaagtt
     6481 tttaatgtta ttattttgac cagtcaactg gaaaccaatc aaatggctgc ggttgtataa
     6541 ccattgtccg tcacttaatt tcttttgttt ccaacctgtc ggatcaatat acaaaggttc
     6601 tctcttttca gtaggaaaca agtcttttcc caacatcgca ttggcttgtc ctactctgtt
     6661 caatttgtct atatttgaat agctttccca tgtccctttg gaaagactaa gatcctcttt
     6721 tgtaaactcc ggctgattcc cattgatttc tatttcttga tgttttccat catattctgg
     6781 cacatccgct acttgagaag cttgcggtgt ggaatcaaaa atggttgtaa tttgttggaa
     6841 gatatctgaa tctttttgtg tggtggaaat accgagtgcc cctgcaatca cagcagcgac
     6901 gatccccacg atcgccttgg ttgttttttt gctcatttct ttactccttt actaacagtt
     6961 ccctaaatct taacatatgt tcccctataa ataaatactg gaaagaaata gaaacagtaa
     7021 aaaaataaaa ccagttttta gacggcttaa gaatacaaaa aaagagacgg attcatcgtc
     7081 tctactcaat cacaaaacac tatttacaag gtatttatta tcattcaaaa aatttttctt
     7141 aatactcaaa actaaacggc ttctgtacga cctcttggta aacagtgttc aaaagcttgc
     7201 atctgcggta ataagtccaa ccaagtcaaa aacataagaa gcttttttgc ttgactattc
     7261 ttattacttg atgcagggca gctttttcac agcctctttt aagcgggtga cgggaatcga
     7321 acccgcgaca tcagcttggg aagctgggat tttaccacta aactacaccc gcgatacaaa
     7381 aacagtatac gcctctttca ctccttcctc aagttttttt attctttacg cttccgatat
     7441 gttttgtagt aaaataaaac taatcaatca attattggag aagatgctat gacgggaaaa
     7501 gaattagatc tgttgctgga cacttgttta ctcgctggga aaatcatgat ggaaagcaat
     7561 gcggagatgt atcgggtaga agatacgatg agtcgaattg ctttagcttc tggaaatttc
     7621 cgtttggtca gctatgtcac tcaaaccggt ctctttattg gtcttgatcg tacctcgacc
     7681 atccgaatgg aacaaattac caaccgttcg attaatttag aaaaagtagt aaaagtaaat
     7741 gatctttcac gtaaatatgt tgcttcagag ctaacattag aagaacttta ctgcgaactg
     7801 caagcaatcg accgagaccg ttctttcttt cctgtctggt tgcaaatcgt tagcgcggca
     7861 gtgatcagtg gaacaattat ggtgttattt ggcggaaaga tgaccgattt acctgtgact
     7921 ttagtcattg gaggattggg atacgcagct tatctatata gtttgaaatt ttttagaatc
     7981 aaatttttat cggaattttt atcttcttta ttgattggca tagccgctat ctacagcgta
     8041 cgttttggct taggaagcaa tcaggatcta atcattatcg gatgtgtgat gcctttagtt
     8101 cctggagttc aaattacgaa cgctatccgt gatttgctag ctggacatta tgtctcagga
     8161 gtttctcgcg gaacagaagc gatgatgact gctacgatga tcggttttgc gattgctttt
     8221 attttccagc ttttttatta ggaaaggaac acaagtatgg ggacgatatt tattcagttt
     8281 tcttttagtt ttttagcgac tgcggcattt gccgttatta caaatgtacc taggcgttca
     8341 ttgatttgtt gtggtctttc tggtgctttt ggctggatga tctactggat ctgtgttcaa
     8401 ataggcggat ctgctgcttt tggttcattg cttggttctt taggagtagc attcatcagc
     8461 gatcttttct ctaaacgttt gaaaatgccc gtaacgattt tcaatattcc cgggatggtt
     8521 cctctagtcc ctggtggttt agcttatcaa gcggttcgaa atctcgttac tgaatcctat
     8581 cgagaagcgg ctcattatgc ggtccaagca attatgattg ctggagcaat cgctttaggt
     8641 cttgtattat cagaagtctt taaccataac atccgtaatt tcaaagtaaa gcgtgacgca
     8701 atcatgatga aacgaaaaaa acggcttttt aaataaacga agtgtaacaa agaaaaggtt
     8761 gtgaaagaga cgttcagcct tgagcgatga caaaatatct cacaaacatt gcgacaagta
     8821 accgcaggag caataagaag taattatgag tattaaaaaa agttttgtaa aaatagcttc
     8881 tcacattttt acataaaatt gacttaatac tgagaaactg acttttgaac accctctatc
     8941 tgtaaaaaag gggctgcgac atgatttttg tcacggcccc tcttctttat tcaaattttt
     9001 tccaaaacgt tcgttgataa taaacgatcc atagagcgga aagtattaga actaaaccga
     9061 tcacaggaat attcactaac caaaatggat aaaacaaagc caacattccg tataagacga
     9121 taagaataat actgatgatc aatgaaccca tcatagttgc gactaaaccc aaacttcctg
     9181 aacctctgtt aaataattgg ttaatattgg tccagttcag cagcagcaac cgataatctc
     9241 tagcgaaata acgtagtgac aataagtagc agccgaataa agaaccaaat agcaagctag
     9301 ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa taatgcgatc ccaccagtta
     9361 aaaaactctg caatatccat ccgaatctga acttttcttt taaatattgg ttcatagaga
     9421 ttggcaaaga acggataaac agaaaatttt cttgatccaa agaaataaga ttgctgataa
     9481 aagaagtctg attaactgtt aagaaagcca atgcgatccc taccaggaaa acaacaccga
     9541 ttaacttatt atctaaatca gttaatttta tttcgcctcc tagggcaaaa gcaagaataa
     9601 aaatgattgg agttaacaag gaagaagaaa gaacttgcat aatcaaatta ggttctttta
     9661 ctaattgagc attgtaacta aataaaagct gatgcacatt ttggttcttt ttatgggttc
     9721 tttttatttt gaactcactg tttgaatcag tcagttgctc tgtaagcttt ggtactatca
     9781 gctgttttac agcaaggaaa aatcccagag ccgtgatagc tagtattccc caacccaata
     9841 aaccttgttg agaaagtggc tgactggcag catcatagaa tggcaataat atcgaaatcg
     9901 gatgccgatc tgttaactca ttttcaatag tccccgtttg atgattcatc cacatgattc
     9961 cagcaaatgc gatcagcatc gagctgccaa gcaagatact agaaactgtt tttctatgtt
    10021 ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa actagccaga caaaaaagaa
    10081 taatcaaaaa tagagaaaac aaaaggattg agataatgat agtaagcaaa atccctagat
    10141 cagctcgcca tccggttaaa ataaacatga ctagaagcgg aaaaacaaaa ggaatgatgg
    10201 tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc ttgtccaaaa ggcagaggca
    10261 aaaatacttg caggtcattt ccttcaaaaa agatattata aatgactgat atactttgag
    10321 ttaaacctaa cataccaaaa agtgccacgt aataagtaaa gaaacctggc aatttaacaa
    10381 agttcatagc aaacatagca aatccgtaaa tcagtaaaaa aatcagccct gaaaaaagat
    10441 tttgagacaa aagatagata gttaatcgct ttcccttttt tcctttctct cttgctttat
    10501 tcgtgagctg tggattaaca tataaaagat taactcgaat taattctaat aattgtcgct
    10561 tatacatacg gtccatctcc tgtcgcttgc cttccagcca ttttcaaata aatcttttct
    10621 agcgtttcat caggtgagcc attcaataat tcactaactg ccccctgata aatcaattca
    10681 cctttcttca aaatagccag tttatcgcat aactgttgtg cggtatccaa tacatgtgta
    10741 gaaaaaatga ctgtttttcc ttttgcggca tgagccttca tcatttcttt caaatcaaat
    10801 gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata cccaaatatc tggatctggc
    10861 agcaacgtgc cgatcaaaat cgttttttga cgcattccat gtgaaaaact tgcgattgtt
    10921 tcattttgat gactatacat atcaaaaagc gctgtcaatt ctgctaaacg cttttctttt
    10981 ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc aatattctcc agccgtcaat
    11041 tgtaaaaaaa tatctgggga atcaggcaca taaccgattt tttgtttgat actcaatcga
    11101 ttttccgtca atttcattcc atctactaaa atcgttcccg atgatggttc aataatacta
    11161 actaaacttt taatcgtagt tgattttccg gcaccattat gtcctaaaaa tccaaaaatt
    11221 tccccttgtc ttatcgagag agacaagtct tttaatgctt gttttttccc gtaactttta
    11281 cttacattga tgaattcaat catttctgta ctccttttgt ttgttattac tttttattgt
    11341 acccttcgtt attggtggag taaatgtcaa aaagagtgca taacaaaaaa ggatagaaac
    11401 gactaggctg ctacttgggt tgttctcttc tacaggaaaa cactcttcta acaggtaaga
    11461 tatgagtctt tgttttcttt aaaatgattc attttctatc ttttcttgca taaaattctt
    11521 tagtttatca atacttctct ttcccatctc tagttgttct ccatttgcaa atgttactat
    11581 ttcgttt
//
LOCUS       DAFGZE010000090        11558 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.093, whole genome shotgun sequence.
ACCESSION   DAFGZE010000090 DAFGZE010000000
VERSION     DAFGZE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.093"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(366..1484)
                     /locus_tag="LTU54_002547"
     CDS             complement(366..1484)
                     /locus_tag="LTU54_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297206.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1955..3262
                     /locus_tag="LTU54_002548"
     CDS             1955..3262
                     /locus_tag="LTU54_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL8297207.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3381..4580
                     /locus_tag="LTU54_002549"
     CDS             3381..4580
                     /locus_tag="LTU54_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBL8297208.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4607..5875)
                     /locus_tag="LTU54_002550"
     CDS             complement(4607..5875)
                     /locus_tag="LTU54_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HBL8297209.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6038..6684)
                     /locus_tag="LTU54_002551"
                     /pseudo
     CDS             complement(6038..6684)
                     /locus_tag="LTU54_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7092..7595)
                     /locus_tag="LTU54_002552"
     CDS             complement(7092..7595)
                     /locus_tag="LTU54_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBL8297210.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TELSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7718..8482)
                     /locus_tag="LTU54_002553"
     CDS             complement(7718..8482)
                     /locus_tag="LTU54_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBL8297211.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVISLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8590..8865
                     /locus_tag="LTU54_002554"
     CDS             8590..8865
                     /locus_tag="LTU54_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBL8297212.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9303..10253
                     /gene="yidC"
                     /locus_tag="LTU54_002555"
     CDS             9303..10253
                     /gene="yidC"
                     /locus_tag="LTU54_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBL8297213.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     IDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(10432..10632)
                     /locus_tag="LTU54_002556"
     CDS             complement(10432..10632)
                     /locus_tag="LTU54_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297214.1"
                     /translation="MGGISALSFMENGVLCGRATWAKGVAPFVTEGEEKASSWMLEEG
                     KQNIKELSTLLEETATPVYVDR"
     gene            11437..>11558
                     /locus_tag="LTU54_002557"
     CDS             11437..>11558
                     /locus_tag="LTU54_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297215.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNV"
BASE COUNT         3493 a         2423 c         2037 g         3605 t
ORIGIN      
        1 tttaaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttca caaaaatttg
       61 agaaataatt tttgtcaatc tcttcttatt tcttgaagct gaccacttct gtcacaacct
      121 cttccttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccacca aacaaaaaat
      181 atgaagagct atttttgctt gctgttctta ttacttgatg cagaacagct ttttcacaac
      241 ctctcataca cggtgtttaa tcagctgacc ttcggtatta gctcaaaaaa cggaaaaata
      301 tgtgaaacta tttttccgtt tgtttctcta atactcaggt caaaacactg atttcacaac
      361 ctcttttaat aacgaagaat cgctgtcaga ctcttttcat ctggcgcatc tttttgatcc
      421 aaaataaaca ctttcccgcc attaatgatc acatcatctg ctaatgcatt cagcacttga
      481 cgccgatcat attctgtttc aggatcttca ccgaaaccat cagctaagtt tgacgtagcg
      541 ataaataaat gtgacacttt tccttctttc gcagctggaa cgatatcaac taactgatcg
      601 atgaatttct tatctaagag tttattgtat tcttttgatt cttgagctgc gagttcactt
      661 gacagcttct tcgccccttc ttcgatatct ctaaaggata gctgcgcagg agaagcagaa
      721 atcgcaacgt ctgcacaata atagggattt ttagcaattt ttttgaacat cgtctgattt
      781 tctggtaatg caaataaata aatcggccat tgttcaggat tttcgaaaga atccttcaag
      841 tacgtatcaa cagcttggta atagttgacc caatcgatct ctacttcttc atcttttgga
      901 tttacgccat ggaaagttac tccatctcgg gctccgcctc ctccattaga agagaagttc
      961 aaaccgccac ctgtcagttc agatcccaat gcagtaacga catcaaccgg tgccccttct
     1021 ggtagagata cttcttttac ttgtttgttg ctgacctcat acaatttcat ggagtcgcgg
     1081 ttcaatccca ataaataata agaatagttg aactgcccat ttttgataat agccagtaaa
     1141 taaggaagat cacttacata gtattgatcg tctaccggga tactcaaacg atggatatag
     1201 gtctggtcag cagttataat gaccgctaca ctcgtagttg cactacgcca aaacgaagga
     1261 tctcctaaca aattttcaat tttttcttgg aacggggacc agtcatgttc ggaatatttt
     1321 ttctcaaaac gtttctttgc ttctttcgca aaatttttga atttgatctg atctttttcc
     1381 acatcttgat gggcgacatg cgtattcagc atgatcgtga cgaatggtcc tttagcgcct
     1441 tctgaagtca attgtgtcaa taattcttta tccttttttg ccataaacgt acccctccta
     1501 aaaaaatcat tcctttgttt ctatctaaag tctaccacaa cactattttt ccatacaata
     1561 aaacgtatct aaaaaaataa aacggtttct acggttttaa atttagctat ttttttaagt
     1621 tatcttcata atttcctcaa ttatttagcc ccagtcttca actattcttc atgaaatgct
     1681 gataaactaa gaaaaagaat aaaagaggtg gatcttatgc atttgattat gattttcagt
     1741 ttattaagta ttatcagttt atttgctttc agcgcttccc gcttattaga tgcggaaaca
     1801 cagcctattc ctgtaaaagt acgtaaaaac gaccgctaaa cttccataac gcgcgttctt
     1861 atcctaaagt tttttcagaa acattgaaac tctctcccct ctctgataaa attttaattt
     1921 gtagcgaaac aaattaatta caaggagatc ggatatgtcc ataaaaaaaa cacgtctagt
     1981 cctattattg gctagtctca ttctttgcct tgcttctttt ctaccagctg ttgctgtgca
     2041 agctgaagat acgttcaaag taaatgcaaa agcagctttt gctgtagatg cgcaaagcgg
     2101 taagattctt tatgatcaag atggagaaaa aaccatgggg atcgcttcaa tcacaaaaat
     2161 catcggtctt tacatcgtcc ttgatcaagt aaaagaagga aaactgtcat ggggtgataa
     2221 agtttcgatt tcagattatg cagaaaattt gagtatcacc ccagatttat cgaatgttcc
     2281 tttgcataaa gaaaacacct acactgtcaa agaattattc gactctgcaa tcatccagtc
     2341 agccaatgct tcaatggtgg ccttggcaga aaaaatctct ggcagcgaag ctaaattcac
     2401 tgaacgaatg aaagaacaat tgaaagattg gggaatcaaa gatgcaacaa tcgttaatgc
     2461 atcaggattg aacaactctt atcttggcga aaaccgacca gaaggcactg gagaaaacga
     2521 cgaaaatcaa atgtctgctc aagatgtggc catcgttgcc cgccatttga tcttggattt
     2581 cccagaaata ctggatgtat caagtactac gacccaaatg tttggcgaaa atacgcaatc
     2641 accagtggaa atggtcaact ggaactggat gctcccagga ttcgttaatt acaaggaagg
     2701 cgtggacgga ttaaaaactg gtacgactga gcttgcaggt gcttgtttcg ttggtacgat
     2761 cactaaaaac gatcaacgga tcatcactgt cgtattgaat gcggacggcc atgcggaaaa
     2821 cccgagtgcc cgcttcaatg aaacagaccg tttgatggac tactgctatg acaattggtc
     2881 agaaaaagag ctcggaaaag ctaacgcaag tattcctagt ctaaaaacca ttgatgtaaa
     2941 agacggaaaa gaatcaacag ttcctgttgt gcttaaaagc cctgtaaaag tatgggtacg
     3001 aaacgatatg gatactggca aattaacgat cacgccaaca cttgacaaga agcaagtcac
     3061 tgacaatgag atccaagcac ctatggataa aggtacaaaa atcggtaccg ctacgatcac
     3121 cttggcagac gataaattag gctatttaga agacaacaat gcaccatcaa cagagatcat
     3181 tacggataaa acagtggaaa aagcgaatat attcgtgatt ggctggcgac gcgtcgcaga
     3241 atttttcggc aacttgtttt aattgaacaa taagaaaaac atctttccat cggaaagatg
     3301 tttttttgct ttttctagtt taaataacag cttttttcat ctattttcgt tatactgtat
     3361 aagaaaaagg agttgaattt atgaatctaa ccagagatct gatcttttta gttctctatc
     3421 ttattggggt attctctatc gtttattggc gaaaacagaa gaaggatcct tccttgttct
     3481 tttacggcag tacgtggaca tttacggtaa tcttaactta ttttgtcgtg ttggaaattt
     3541 tttattcgac cttttatttt ttgattccat tgttgttctt tagtatcttt gctttttctt
     3601 atttcacaga aaaacgaagg ctactgaatg gtcttttatt caatgtttta ctcatcagtt
     3661 tcggcatcta cttgtttgtc ttgctgtatg aaacgcaaaa tatttttctt ggcggactga
     3721 tcgccttgat cacgattcca ttgctacttg tccttttatt tggtatttat ggattgattg
     3781 tttttctttt ttggaacggg gtcacagttt tacgtagaga atcgcattca ttagctaatc
     3841 ttttaacgtt gatactagcc atttttctta cactgtttct tgtttttgac tttttcttgt
     3901 taaaatattt gccgcaatgg ataaatgctt tgtttttctg cgttcccctg atcttgatct
     3961 atctatttat cgtattctac aactttttga ctgtctcatt cttgtatcag ttcaaccggc
     4021 ctcgatacaa tcaagacttc atcgttgtgt taggcgctgg attgatcaat ggagaaacag
     4081 tttctcctct tctagccaag cgaatcaata aggcaattgc tttttatcgg gctcaaagcc
     4141 gtgctacatt aaatccgccc atcttattga tgtctggcgg gcaaggagca gacgaaaaag
     4201 taccagaggc cattgcgatg aaacaatatg ccatggaaca aggaatccct gaaagagaca
     4261 ttcttgtcga aaccaattcg acaaccactt tggaaaacat gctctattca aaggaaatca
     4321 tggatcagca aatgaaaggg ccataccgcg cgatctttag ttccaacaat taccacattt
     4381 ttagagccgg tctttatgcg cggcaagcca aattgaaagc aaatggtatt ggtgcaaaaa
     4441 cagctttcta ctacttgcct aatgcgtttt taagagaata cctcgccatc ttgatgatga
     4501 acaaaaaaag acatatgatc atttgcggat tgcttttcgc aggctccgca ttcttatcgc
     4561 tgattacttt acttttctaa aaaaacaagg tgtgacaaaa catatgtcac accttgtttt
     4621 tttctttcat tctcttaact attggcaaaa agagtatccc ttttttgacc gctacttgct
     4681 gagcagaata aataccgctg ttgcctgaac acataaagct gatcaagcag atcataaaga
     4741 agtatactgc cgcttgtgaa ccgaacaatt cgatccccat cacaaagcag gcaatcggtg
     4801 tatttgtcgc accagaaaat actccgataa agcccaatcc tgcaagaaaa ggaacagaaa
     4861 catggagcaa aggcgctagt gctgcaccaa gcgttgcacc aatttcaaac aatggtgtca
     4921 cttctccccc ttgatatccc gcgcctaaag acaaaactgt gaagaataac ttcccaataa
     4981 aatcatagac atgtgcctgt ccattaaatg attcttgaag taacggaaga ctcaatccga
     5041 gataacgttg tgtttgcaaa atcaatgctg ctgccacaac tacagctcca cctatcgtat
     5101 ttcgacaaac aggatgagga atccattgtg catatcgcgc ttttaaaaag acgatcgaac
     5161 ggctgaatac ccaaccaatc aaaccaaaag caatcccagc aacaaaaact ttgaagaaca
     5221 gcaacatact ccattcagga atcacaccca tttggtaatg tagatgagaa acaccatagc
     5281 tttctgtcat gaaatttgca aacagtgcag caaagaagct agggaagatc gcttctgttc
     5341 ggactttccc gattgccaac acttctaatc caaataatgt tccagctaaa ggtgtgccaa
     5401 atacagaact aaaacccgca ctgattccac tgataatcaa gatttcccgt tctgatttat
     5461 ccaatcggaa caaacgtgcc gtattatctg ctaacgctcc ccccatttgg actgctgttc
     5521 cttcacggcc aacagatccc ccaaataaat gggtagttat ggttccaaac agtgtaagtg
     5581 gaataagccg caacgggatt ttttcttctc caccatttcc ttgatcgatc actaagttgt
     5641 ttccacgact agaaagtccg ccgtgatacg catagagata agcaaaaagc gcaccactaa
     5701 ctggtagtag acaaaggagc caaggatgtg atagtcggat atctgtcaca actgtcaggc
     5761 tatttaagaa aaaagcagac aagctcccca tgaatatacc gagaatcgct gtgatcacca
     5821 gccacttcag catatacact aaaatgcgta ctggactcgg taattgtttt tccataaaaa
     5881 attcctcctc aataaaaaaa cgcctactaa agaaaggggc tgtccccaga ctttagtagg
     5941 cgtcattagc tatctttcgt tagcatttaa aggcgaacac catcacctat tcacttccat
     6001 taaaagatag catatttttc tgtaaaaaac aataaaatta tcgttctttc tttacacgtt
     6061 cagcaaattc atagaaagga gccaatttcg ttaactgttg attacattgg tcactatcat
     6121 gacaaatata attccccttt ttcgtatacg tgccatcaga acctgctttc gttgtagaaa
     6181 gaaaaagcga aacattcgat gttttctgac agattgcaca gattccctta tttatcgttg
     6241 gtgataaagt accgctaatc ccttttaatt tttcatcttc gtaatagacc atgaattttt
     6301 tctgcgtacc tggatcattc cagccgatgt acgtatgttc ttgcaagtcg atttcattcc
     6361 aagctggttg tttgagtttt ttcacttttc ggaataattt ctcgatttgt ggtttgctag
     6421 gctgcataaa cggctgaacg atttgcttca gctcctccaa tgctctttct gcttgcgctt
     6481 ttgtcagttt tacgtctaaa actgtcgtta aaaatgtatc gattctggca gttcgtgatc
     6541 taatatgtct cgaatctttt cagctgctag tgcctgaact gtttgtaccg tttttggatc
     6601 attgacagat gagtacgcat ttaataactg ttctacttct cttattataa aaaaatattg
     6661 gtatggtttt attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
     6721 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
     6781 aaataagata cgttttgcat cttatcatca ccccctttaa tgcttattat tctatgtaca
     6841 tgattcattc gataaataaa agtaaaccgt atcttgaaca gatagtcaac tgcaaacaga
     6901 aaaaaggact gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaatgaat ggtgttcaaa
     6961 agctggcatc tgcggtaata agccgaaact tcacaaaaat ttgagaagca attttcgtaa
     7021 attccttctt atttcttgaa gctgaccact tctgtcacaa cctcaatatg gtctattatt
     7081 ggttttatct attaaatcga ggattctttc ccaaagtaac gttgccatct cttacgcata
     7141 cttgctgaca taggctgagc ggcttctttg actgcgcagt atttgtctac gaatgttacg
     7201 atatatcctt ctttgtactt aggcggtgca atcgttgcgc cccacatgtg cttcaaaatg
     7261 atatctcgct caagatccga caattcggtg atcttttctg cattctttac agcaattctt
     7321 ggatggatat acgcatgtgt gccttcatca aatttcgtcg tacgccaatc atagtagaac
     7381 agatcatgca aaagtccagc acgcgctgtt gctttagcat tgccacccca tttttttgcg
     7441 agcagataac tatagtaaga aacactgatg gaatgctcca aacgagtgga atgaacatgc
     7501 tgtgtgtaat tcgctaattt tttcactgct tctgtttcta aaagatcttc tacataagac
     7561 atatattctt cgtcttcacg ccatttttct gtcataatta tcgatctgcc acctttttga
     7621 atttcatttt cttctgaaat tgatctaagt ataccaccag tgttcggatt attctagtcg
     7681 ccttggataa aatagccaaa gcttaatagt tccttaatca taaagaaaaa tgataaagaa
     7741 caatccctgc cgcaacccca acattcaacg attccgcatt tcctttcatc gggatatata
     7801 cgttctgatc agtcaaagac aagatttcct gacttacccc ctgaccttcg ttccccatga
     7861 ttattgcgaa gttatctgat ttttctactt tgtctaatgc gtgtgcttct tcatttaatt
     7921 ctgtgccata cactgtaaac cctttgtctt tcattaaaga aatcacttct gtcaaatctt
     7981 tttgcaacac aggcaaatga taattgcttc cctgcatgct gcgcagcacc tttgtactat
     8041 aaatatctgc cgttcctttg cctaataaaa caccagccat atcaaatgca tctgccgtac
     8101 ggatcatcgt ccctacattt ccaggatctt gtacattatc taacaacagc catcctcctg
     8161 aaaaatctgg cgtgccttca gccgttaacg ccatcactgc taagattcct tgtggtgtag
     8221 gtagatccga tacagcatcc atgacctcat ccgaaactag aaaagtagaa atctctttgt
     8281 ttgcggccag ccagtctccc cattctttca tgcctcgctg tgtcacaaaa agttctttta
     8341 tttctgcatc agacgacgca gcttcttcaa tcagatggaa cccttccaaa agatattctt
     8401 ccttttcttc ccgatatttc tttttatgta gtttttttaa ttctttgatt cttggatttt
     8461 ttgctgattg gatctctttc acattcctca cctccccatg attatatcat ggaataggtg
     8521 cttgagcttg tttcatccta aaaaaatcgg tatcattaat ttataaaata ggaaagaaag
     8581 gtgacgaaca tgcgcaaagt taaaatgaac gtccaaggac gcgtccaagg cgtaggattt
     8641 cgctacatga ccaagatggt cgctgatcaa ctaggcgtga ctggaacggt aaaaaacgaa
     8701 gatgacggct cagtcacgat cgaagcaatc ggcaatgacg acatcatcca gaaatttatt
     8761 gaagaagtca agaaatctcc gagcccaagt ggacgagtgc agtatgtgga tgtccaagaa
     8821 gacccgatga ttgaggaacg aaagaaattt gatgtggtgg gataaggttg tgaaaaagct
     8881 ggtctgcatc aagcattaag gacaggttgt gaaaaatagc tctccatatt tttcaacagg
     8941 ctggacttaa tgccgtagat gcaagctttt gaacaccgtc gataaggtta ttcttgaggc
     9001 gctttgcctc aaaaattaag gacagcagtg aaaaagctga tctatcactt gcagtgagtt
     9061 catttgactc cgctttttcg ggcagaaaaa aagtttttag tttattaaag taagaaagaa
     9121 acacttggaa tcattctact ttttcctagt atttctttct tgattttttg tctttttttc
     9181 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
     9241 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
     9301 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
     9361 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
     9421 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgaaaat ttcaattggt
     9481 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
     9541 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
     9601 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
     9661 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
     9721 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
     9781 atacagaagg tatccgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
     9841 tacttgttgc cattgcaggg gtcgcttatc tgcttcaagg atacatctca acgatcggta
     9901 ttcctgaaga acagaaaaag acgatgcgta cgatgctgat cgtctctcct gctatgatcg
     9961 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    10021 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    10081 tggcagaaga gttgaaacaa aacccaccaa aacaagtcgt cactccaaga attgatgtga
    10141 cagaatcagc taaaccagct gacagcaaac aagcaaccaa acaaatcaag caacccaaaa
    10201 acagtacaaa caataaaggc cgcaacgcag gaaagcagca gaagcggaag tagatagtaa
    10261 ataagagtgt aataaaaatt cattaacagt tttagcaatt aaaaatatcg accaagattt
    10321 ttgaaaaaga aatctggtcg atatttttat ttaagtgaat gtttaatttg aatcatcgat
    10381 tgatcgctat ctaattgcag cagcatcttt ttatgaagaa acaacttttt cttatctatc
    10441 aacgtagaca ggtgttgctg tttcttctaa tagtgtactt aattccttaa tattttgttt
    10501 tccttcttct aacatccaac tactcgcttt ttcttcgcct tcggtaacaa aaggcgccac
    10561 tcccttcgcc catgttgctc gcccacataa gacaccattc tccataaagg acaacgcgga
    10621 gataccaccc atctaaataa catccataaa cctttctagt agaagaatag tgatagtatc
    10681 catccctttt tctactggga atacaattac acgattcaaa gcttgataca caacatttcc
    10741 cttcttataa accgctattg taaccgaatt gttttgaatc acctttttgg gatagaaact
    10801 ggctcctttc cgcaacttct taaatgttgg tttcttttat actttctttg tagcaactac
    10861 aaatttcttc aaatctctaa atgtttgcat aacaaccgcc tttctgactt ttactcagga
    10921 tggtatttaa aaatcagcaa aataaggctt ctctccatct tttttatttc ctagctgctt
    10981 taaagacact cttgctgttt tttaatctct atcaaggggg ctctgaatct attttgatga
    11041 acttatctta ttaatttctc aaatgagctg tcagacacaa ctgtattttt tcaatctcat
    11101 accggacaac tatgccagct taaaaaaatt tcctctggca aaacttcttt attgaaaaat
    11161 gacaatgaaa aaagaccatt ctttttgttg aatgctcatt gtgtaaacat atttcctgcg
    11221 actgctcttc gaaccattct cagtgcaatc cctaatttat ctattaaatc ttcttttagt
    11281 tcctcatctt tcatattcaa aatttccttt gtatgttaca caagtttgtt ctctaaataa
    11341 cgcgaattgt aaaattaagt tagaaaaata aaaaggcatt tatggtacac tcaaattgta
    11401 tttccgacga aagaaaacaa aggagtgtaa ccataaatga cctacaaaca tcttaccata
    11461 gacgaactga caatgataga atcatattat cttcaacata ataagccggt tgaaatcgct
    11521 aaccgaatgg gacgtgctat acaaactatt tataatgt
//
LOCUS       DAFGZE010000091        11247 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.094, whole genome shotgun sequence.
ACCESSION   DAFGZE010000091 DAFGZE010000000
VERSION     DAFGZE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.094"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(214..387)
                     /locus_tag="LTU54_002558"
     CDS             complement(214..387)
                     /locus_tag="LTU54_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297216.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
     gene            complement(390..788)
                     /locus_tag="LTU54_002559"
     CDS             complement(390..788)
                     /locus_tag="LTU54_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297217.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKYT
                     SMKPCILDWQWLIDAVLDVKCSKYNITLGC"
     gene            1004..2059
                     /locus_tag="LTU54_002560"
     CDS             1004..2059
                     /locus_tag="LTU54_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HBL8297218.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2079..2423
                     /locus_tag="LTU54_002561"
     CDS             2079..2423
                     /locus_tag="LTU54_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297219.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDSKVAEVKNKPTDHNPIQQEHPSTVTSGHSAKKEKPML
                     KGFNAPSNKDLL"
     gene            complement(2604..2900)
                     /locus_tag="LTU54_002562"
     CDS             complement(2604..2900)
                     /locus_tag="LTU54_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297220.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETRSSLVDK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(2904..3722)
                     /locus_tag="LTU54_002563"
     CDS             complement(2904..3722)
                     /locus_tag="LTU54_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBL8297221.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            3942..4163
                     /locus_tag="LTU54_002564"
     CDS             3942..4163
                     /locus_tag="LTU54_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBL8297222.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4165..4431
                     /locus_tag="LTU54_002565"
     CDS             4165..4431
                     /locus_tag="LTU54_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL8297223.1"
                     /translation="MSYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            4544..5083
                     /locus_tag="LTU54_002566"
     CDS             4544..5083
                     /locus_tag="LTU54_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297224.1"
                     /translation="MYNKSVNKLINLQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            5574..5858
                     /locus_tag="LTU54_002567"
     CDS             5574..5858
                     /locus_tag="LTU54_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297225.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            5865..6110
                     /locus_tag="LTU54_002568"
     CDS             5865..6110
                     /locus_tag="LTU54_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297226.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            6132..6386
                     /locus_tag="LTU54_002569"
     CDS             6132..6386
                     /locus_tag="LTU54_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297227.1"
                     /translation="MNYTDEEQITLEYVFTNEEGEEIEKVLIISTKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            6468..6653
                     /locus_tag="LTU54_002570"
     CDS             6468..6653
                     /locus_tag="LTU54_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBL8297228.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            6720..7115
                     /locus_tag="LTU54_002571"
     CDS             6720..7115
                     /locus_tag="LTU54_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL8297229.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            7105..7482
                     /locus_tag="LTU54_002572"
     CDS             7105..7482
                     /locus_tag="LTU54_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297230.1"
                     /translation="MKSKKETEPMKSTHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            7504..7878
                     /locus_tag="LTU54_002573"
     CDS             7504..7878
                     /locus_tag="LTU54_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297231.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(8184..8582)
                     /locus_tag="LTU54_002574"
     CDS             complement(8184..8582)
                     /locus_tag="LTU54_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL8297232.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(8774..9541)
                     /locus_tag="LTU54_002575"
     CDS             complement(8774..9541)
                     /locus_tag="LTU54_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL8297233.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            complement(9576..10799)
                     /locus_tag="LTU54_002576"
     CDS             complement(9576..10799)
                     /locus_tag="LTU54_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBL8297234.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGRTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGTEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
BASE COUNT         3857 a         1916 c         1737 g         3737 t
ORIGIN      
        1 aaataaaaaa aataaggcaa aaaaagtatc aacataaaca tatacatata cacgtttatg
       61 ttgatacact taatttgtcc tttaaaaagc acttttttga tattatgtta ttttataatc
      121 ttattacaag ctgattgtta ggcgttgccc tttcattaca tttattaacg gttattttat
      181 cgtttaattt attaattatt tttgatctaa aacttagctt aacaaataaa taattaacca
      241 accgccagat aaaaatgcga tcattgctaa tacataagca aagataaaaa tatgttcgat
      301 cagcttaaac cctgatgcga ttaagcagac caagtaaaat agaaattctc ttacagataa
      361 tggtaatttt ctaactagca tactcataat taacacccca aagtaatatt gtattttgaa
      421 cattttacat ccaatacagc atcaatcaac cattgccaat ccaaaataca aggcttcatt
      481 gaagtatatt tttcagatga aaagatataa tataatttct tcggaataat attttcagtt
      541 gttgggatca agctaaatct aataccgtct aactcatagc taccagcttt ctctccatta
      601 aaaatttcct tagcactttc ttctaaagaa tcaaaatatt tttcgacagc aacctttgaa
      661 gcttttacta ttgaagcatt tcccttttcg ataaaatctg cttttgtttt accgacaata
      721 agtaattctt gatcagtaaa atcttctaaa ataatggtag tgtaattctt atttcttctc
      781 aatcgcatca ggatcactcc ttaatataat ttatgttttt ataatatcat aataagataa
      841 atttgcaagc atttatttac acctttttat ttacacttta ttataattaa ttatactttt
      901 atgattgcat ttttttctta taaacagttg caactaatag taaataggtg taaactattt
      961 tcgtaccgaa ataccataaa cacaaacaga aagaggtcat accatgaaag aaccattatt
     1021 gttttccgtt gacttaggga ataaatcagt aaaagaaatg agtacacacg tttctccact
     1081 ctcacttccc tctcgtattt tgaacaaaaa tatcattaat tcaggacgtt tttctaatat
     1141 aggttcctca ttaaaacgat cactaaacgt ttctgaatat agcctagtaa acactactga
     1201 aagctattac tttgggaagg atatacacga gctaggaaaa gatgactacg tgatagaatc
     1261 actaggtttt gggatcagca gatacaagca ggaaacctac caaaatatcc tatctttctc
     1321 tttagctaac ctagcacgtt tagaagatca atctgatgac gttttaaccg ttaatctagt
     1381 tttaggtctg ccttcatccg actataccga aacaatcgtc aaatacgtga tgcaacaagc
     1441 cacacgacaa cattcagttg aagttgacgg taaggcttat aatataaacg tagataccgt
     1501 ttgggtactg ccacagccga ttggaacact atataacctc ctacttgctg atgatggtag
     1561 tgtacaagat gaaacattga tcagtcagaa gatcggtttt gttgatattg gcggtggcac
     1621 gaaacttttt gatgttgtcc aaaattttga aattgacgaa aacaatcgtg aacaaaagga
     1681 taccggcatt tttactttat ataatcgtat cgtaaatatg cttgaccttc cgatcagtcc
     1741 taatgctttc caacttgaaa agattatccg agaaggcaaa gaaaaaggcg tatatctatt
     1801 taagccaagc ggaaatgaaa gttttgatat ttcagaagta gttgaaagag aaataaaaac
     1861 atacaccaat atggttgttg cagaagtttt agctacattc aaaaacttca caaccttaga
     1921 taaagttgtc tttactggtg gtggagcttc tattatcgac aaacaacttg taaaagataa
     1981 aattccgcat accttgtttg ttgaagacag tgaattttct aatgtaaaag gcttttacaa
     2041 gtttggttta actcaataaa acagcgaagg agatcaacat gaataaggga aaatcagagc
     2101 gttatgtagt tataatcaat agagaaaagg ttagcgagtt acctttagca gagtttcttg
     2161 aatccgaata tgaaacacta ggcgttaaat actctcagac gatcaaaaaa gctttgaacc
     2221 tatatcggcg tgtaaagaac aatgaagtta caattattga agcaggaaac gatagcaaag
     2281 tagcagaagt aaagaataaa ccaactgatc ataaccctat tcaacaagag catccttcaa
     2341 cggttacatc cggtcattca gcaaagaaag aaaagccaat gctaaaaggg ttcaatgcac
     2401 caagtaacaa agacttactt taaaaataaa aaggcgaggt tacaatacct agccttttta
     2461 ttatgtacaa atataaacat aaacatatac gtatacatgt atataaacat gtatacgtat
     2521 ataaccataa acacgtatat gaaatttagt taagctatac ctaaccgctt attccaataa
     2581 aaaaatgatc agctttaaac tgatcactca ccaaaaactt catcatacaa gttatctatc
     2641 atttcgccaa gtaaagctga atctgatcga attcctaact tttcgctgat tttagatagt
     2701 ttctttcttt gcgatggttg aagcgtgaat tgataatttt tcttcttttc agaactatca
     2761 ctttttagtt tatctaccaa agaacttctt gtttctttag gtgtttgttt aggttcttca
     2821 actgcttcaa ccacttcagg aacttcttct acttcagctt cagcgaattt tttaaatcta
     2881 ttttctttca cttcaatcac tccttattct tgtgccgtat caattatttt gttgtatagc
     2941 gtaaacgtgc tttcaaagaa atccttattc ttacgataga tattcttatc cttagcgaac
     3001 tcagcaatat tcttaccttc taagctagtt gcattaagca attccttctc ttgtacatat
     3061 ccaagataat tagataacgt tggtaccaat tctaggaact cacgacttga attagtatta
     3121 tgtttgatcc tgctaccgat cgtagcgtat ttagtttcca tcagtgtttc accagtctta
     3181 atatcaatca actgttttgc tagtgcatca atatctttac caagcttcag caaagcttca
     3241 aaaccatctt tagatggatc agaaatgcct acgacaagat cagatacagc ccaagcattt
     3301 tgagctagta gacctgcatc attatgagta tcaatcagaa ttagatcata cttctcttta
     3361 agctgttcat agttaacaac gatccatcta aacagtagag ttctgttgtt aggcatatca
     3421 cgcatttgca tctcaacttg ttttaaatct tcacctgcta ctaaaatatc catattctca
     3481 tttatagcta aaggttcagc tgatccatga agatataaat gattgattgt attctctctt
     3541 ttcgctttac tgaaagcatc aggaaagcgt tttgataggt tttgcgactt atcttcatca
     3601 atatacagca ccttcatacc taaattttcg acagcatatc cacctagatt gtagattgtt
     3661 gttgttttac ctgttccacc tttttcaatg tttccagtaa tgattgacaa cttcttttcc
     3721 atgattaaac ctcccttatt aaccttttgt aactccatta tacactataa acacgtttat
     3781 gtatatgttt atatacataa acgtgtttat atttatattt gcttttatat tctacaacat
     3841 aaacatgtat atgtatatgt ttatgtgtat gttcatatat ataactattg catttgtcat
     3901 acaatcgtag tacaatgtaa ttgaaaagga gttgataaac tatgccagta ataagtattc
     3961 gtgtatctaa agaagaaaaa gattttcttg aaaaagttgc tgaattcaac ggagataaca
     4021 tttctgaatt tatgcgtagt aattccctgc aaacagcaga aacgttagta gattttgcca
     4081 cctacaagga actaatgaaa gagcatgagg aaaacgatca aagtatttcc cacgaagata
     4141 tgctaaaaga gttaggctta taagatgagt taccaagtaa gatatgaacg tgaagctcaa
     4201 aaagcgctaa aaaaaatgga taagtttcag gcaaaggtta tcctaaactg gatcgagaaa
     4261 aatcttgtag gcactgatga tccaagaaag catggtaaag gtctaacagc taataaatca
     4321 ggctactggc gatatagggt aggtgcatac cgtataatag ctgatattag cgagcatgaa
     4381 gtgacaattc ttatattgtc tattggtcac agaagtgaag tttataagta acagacaagc
     4441 tgataaggct tgtctgtttt tttgttaacc caacaaaatc actgtttacg atcccacttt
     4501 acctaaaaaa gaacaatatc ctcttgttat tatacacatt ggtgtgtata ataaaagtgt
     4561 aaacaagcta attaacttac aaaaggagcg attttttatg ggatggtatg gtgcttattc
     4621 atcagcaaat gaagtgatcg aagaaaagaa agggtacgcc aaaaatggtt tggaaatcgt
     4681 agataccaaa gtaacaaaga cctatggaat tatccttttc aaagatgcag aaggaaccta
     4741 cgatattgac agcttcatct ttcgagatga aatgtacaaa ccaatctact tcttaaatga
     4801 cagtcctgct tactttaaga aaattccgaa aaaatggttg aaattctttg tcggtgatca
     4861 agaaaaaatg gttaagcgac aactagatag aattaaagaa gaaagcaata aaccaaaact
     4921 tgatgactta ttagttgcag gtaaaactta ctcaatctgg gatggatcgt atgtcgctgt
     4981 ttatcagtat aaactcaaac gtagctttat ttttcgacta caagatggat cactcactcg
     5041 gttcactcga ctatcagcag gagatgttaa ggaagtagcc taaaacttaa atacataaac
     5101 ataaacgtgt atacgtttat gtttatgtat atgtatatga atataaacgt attaggtacg
     5161 aacgctttta aatgcttttc tgggctttga ttttagggta gagctaaaac atcaccatat
     5221 gatccaaaac agctcagaaa cgcatttaaa cgtatctgat accaatatta tagaaggagt
     5281 gtatcgtatg attaacctat ttcttttggt gacagctagt acattattct ttactggaat
     5341 atcacttata atttcctatt tgatctataa gaaagccgaa acttacacaa gcaaagcaac
     5401 cggactagcc gtttcatcct ttttaacttt gctgactttt tttgttactc tatcgttttc
     5461 ttttgtttag cacagctgat ccatcagcaa aaaacctctc tttacattat acacactagt
     5521 gtgtataatg taattgtaaa caagctaatt aacttttagg aaggagtgac aatatgctaa
     5581 cagctacatt acagcaacct actcacgttg ctattgcaga aagctataca attaatgcag
     5641 ggatcgttaa acagctgatc aaggatcgag aattcaaaaa gtttaacagt cgtcaagcgt
     5701 ataacagaca gttaaagaaa gctattctaa agcacaaaaa ttccttcatc gggaaattgg
     5761 aaagtgtgga tgttaaaact gtttcaacgg cattttcagt tgttgaagtg actattgacg
     5821 gtcgcttgat ttctctattt gcttttggaa ttaattgata gggagtgtgg actttggaag
     5881 gtaagacttt tgcagaaatt tggactgaat ttattgaggt accaactcgc attgatccga
     5941 taacttctga aaaggtattg gatgcagact tctacaatct caaagcaggt actagccttg
     6001 aatgcgtttg gatgtattta gaagagcatt tcaaaggcgt aacgctcggt cacgtcttat
     6061 accacaatgg tgccgagtgg gatgcaattg gaaatatcca tttttcttaa tacaagaaag
     6121 gagaacacaa tatgaattat accgatgaag aacaaatcac tttggaatac gttttcacaa
     6181 atgaagaagg ggaggaaatt gaaaaagtct tgatcatttc aacaaaagac ttggagaagg
     6241 ctttggctta tgaaccatac gaatcaaccg taactgtcat tgaattctta gatacctata
     6301 cttctaatga tacttgggaa attatgaata tttgcaacgc taataacatt caatttgctg
     6361 aaaaaaccaa taaatcattt tattagtttt gcaataaaac atcctctttt tctattgatt
     6421 acatacacaa caatgtgtat aatagatttt gtaaagggag ggtagctatg cctaagacag
     6481 caagagaaat tgtaaaaatt ctcaaatcta acggcttcaa aaagactgga caaactggca
     6541 gtcatgcaaa attttataat tccaagacta aaagaacggt cgttgttccg atccacaatg
     6601 gtgatgtacc actaggaaca gaagctaata tctggaagca agcagggctt taacagcctt
     6661 gccttcctat ttggtattag gcatactatc ctctcttaca ctagaaaagg agcgaggaaa
     6721 tgaagacaaa aaatattttt tatccggcaa tcttcgagaa agaaggagaa gcttataaca
     6781 tcgttttccc tgacttaccg gaaatatcta cctttggaaa tagtcttgaa gaagcatatt
     6841 taaacgctaa ggatgcttta ggattagcgc tatatagtgt accagatgca gaatttccta
     6901 aaccatcagc agtggaagaa ttaactttga aagccaacca agtggtagta atcatccaac
     6961 taaacattaa acttttccga agaagcttaa atacaaaaac aattagaaaa aatgttagtg
     7021 ttccggaatg gttagttctt ttagggaagg agaaaaacat aaacttctct caactacttc
     7081 aaaaagcact agaagaagag ttgcttgaaa agtaaaaagg agaccgaacc tatgaaatct
     7141 acacacttaa actatttctc tcgaacgtta atcaataatc tattaaaaga tacaactggc
     7201 attagcgtta gtaccaaaca ctttgccaat gatctaatta tttttgagga taataacttg
     7261 cgaattgaga tgtttattga acaggcaacc cctaactcaa tacctgaatt ctgctatacg
     7321 cacactgaaa aaatgaaaca tgcaaaatca gttaaagatg ttgaagcatt aagtgaatca
     7381 ctaacgaacg ctttaaacct cgttaagaag gcaaaattgt tatttgaatt tcaaaccgac
     7441 taccacaatg agttgcttgc tcaatctgaa attttagatt aattgaaaca ggagaagaga
     7501 aagatgaaac aaacacaaac agaaaccgtt aacgatacgc ttgctgatca attagcaggt
     7561 tattataaat tatttgagat gggtaaagca cagcgcttga tccactatct ttggaaatac
     7621 aaaattttgt ttcttttttg ggaatcaaca ctaacattct cagttctttt ttataagtca
     7681 gatttttata atcaacactt agttttaggg agttttttgc ttatctacac cattttgatg
     7741 cttaatttat atacctacat cacacttgaa gaacacttag taattagcga tgcagggatc
     7801 gagagaaaca tcaaaagggt atctttcaag atcacgttaa acaagcctac cgataaaaag
     7861 ctctcagaag cgttctgaga gcttttgaca ttttcttgta taatttatcc taacacatta
     7921 taaagcacct aattcgagct tatatcaagc aaatttgcta tttattactt aatacatatc
     7981 taaaaattac ttgatggaaa actagataca tggtaagcat gaaaagaaaa tcaatttgag
     8041 cagatgcagt acaatacata gcaatttaaa agctctcaga aggcttctga gagcttttaa
     8101 tatattcttt gataatttgt ctttcttaaa tccatacgtc ctaaaacaag ctgtttttgc
     8161 ttaatatgat catctgagag acatcaaatc aacttttctc tcaacaaatc attcaaagct
     8221 ttcgacagat taatattctc ttcagccgct aaaacattta aatagtgttc cacagtcact
     8281 gattttcgat cataattttg cttgtttcga gctttagcaa ctttcgtatt agcttttacg
     8341 acaaataaat taacattctc agatcgcttg attcttgcat ttaactcttg aacaggcgta
     8401 ggcgatggaa tttctttttc ttgcaactct aaaagcacca agtatccttc caatacttct
     8461 tgcgccatct caaaagcatc ttctaaagtt tcaccatgtg tatataagtc tggaaaatct
     8521 gggaatttaa ctgcatattc atttcctttc cattcgaata ctgcacaaaa aaagtatttc
     8581 atcgcattgc acctctctaa ttaaaaaata atttttgctt ttttgtttca aaaaaaagaa
     8641 tcgcctacca aaaaaattga taggcgatca caagctaatt aacctctaaa ggagattaat
     8701 accttgctcc caaggcacta tatatcttag catacgtctg aagcatcttc aaacgagtaa
     8761 gatatatttt gcattaacct aatttttttt ctctataagt tttttcatca aactcactag
     8821 aaatattaaa ctggacagca aaattgtaat ttctgccttt tctaacttta gcaaaatcaa
     8881 tattcagcat tgatttttca ttaatttcct caactgattc ccttaaaaca cgtctatcaa
     8941 aattcgatat tgttttatac tcattttctg ttttcgtaat ctcacgaatt ttaactattt
     9001 caatcagggg attcttatat atttcagctt tctcaaaatc tcctttattt ttggcgataa
     9061 gatatttttt ataatacatc gccaaatatt tgtataacac gatgctatat tttttttgaa
     9121 aagatgctaa gtcttcaaaa acaaagaacg gatacttctt tttgattgag acaattaagg
     9181 gaattacatc ttctgaaaaa ctgaaagata cactatgctc ttctaaatcc caatgaatcc
     9241 gatcaatagc agatacagta gtatacgtct tatatttttt gtctagattc gaaatttcat
     9301 aaactgcttg tctttgcaaa gtgttaacta tggtttttag ttccgtttca agattttgat
     9361 caacatccat gtaattaata atcgctttaa caggtaaatc tactacgcct gttttcttta
     9421 gctttgtggg tgagatataa ctgaacagca attgataaag ttcatacgac tttaaatcca
     9481 ttttcatgtc aattgttgct aagaactcct cattgtactt tgtcagttca tcttttttca
     9541 aaagtttttc cttccttcac gcattttttc agttgttaat cataaaggtt cacataaggc
     9601 aaataactcg taattgattt ttcgagatat ttaacagtat tttttattgt gtaatcctcc
     9661 ttatgagtag caacataaga taggtgattg acaactgatt caaatcctct atctttggat
     9721 aaacgatcat aaagcggata aactttattc tgaagtcggc tcataagttt aagatcgcat
     9781 aagtcggcag aactgatcaa aaagtttttg attagtaatt tagtgtattt gctattcata
     9841 gctttcaagt acgattgatt cattttctct gttttatctt ctgtgccttt taaataagca
     9901 gggtctcttt gttcttgctt atagggtacc ggaactgaca tggtacgtgt aagcaagaac
     9961 tttatatttg tataagtacg acccttcttg ataagctcat agtcaatttt aaaatcagtg
    10021 tatttattca gttgggcaat cggttcttta attacacgtc tttcaaagtc tcgaaaagct
    10081 ttatattttt tctcaatact gaatttcttt cgtaaatctg caatggagaa aacaggattt
    10141 tttaatctat caagtgattt tgtactccat gttaagttat ctctatttgc ttcatacaga
    10201 ttaaaatctt tagtgaataa ttgaaaaaaa ctgattgcat acttgcttgt caagttaact
    10261 acttgatcaa atgtatattg cgtaaaatga tcctctaagt tcgttaataa aggcataatt
    10321 gaactactaa atttaataga tactgcatca gaaaaatcat tgaattcagt aatttctagc
    10381 ggtgagatga ctttgtattc agtcttaccg ttaggtttag ttctttgaat tttaatgaca
    10441 gattgctgat gcaatttttc aataacttgt tttaatcttg aatgttttga agaagatgaa
    10501 ctagaagaca tcaacgcaaa taaagccttt ttagatactt tgacggtatt attaaaaggc
    10561 ggatttttag ggtcaagatg tcctatagcg taaagataaa ttttttgtgg catagcatcc
    10621 atttcaacaa tgctatctac aagttgattc gataaagtaa cagttaaatc aaactttttt
    10681 agattgcttt cggtagtttc aaatttcggg tttacattct tttctgttat tttcatacga
    10741 ttgcctcttc tttctaagct tggtacgagc ttaaaaactt ggaactggta cgattccatt
    10801 gaataagtat agacctgttt tcgatacgtt acaataagaa atgtatcgta aatttataac
    10861 ataactcaat gtcagtaaaa cacattttat tttttccaat caaatttcat tgaaaataca
    10921 gcagaaatcg ctattttaga acattttcaa aataaaaatc cttttcaaaa aaatacgaaa
    10981 ttataaaaat taccaaaatt aaaaaacaaa tatttttcta gtttttagaa ataaattcac
    11041 atactttttc tttttttgga tatattcgga ataccacatc gctttttgtc cacattcaca
    11101 tcgctttatg tccaccctaa aatctattta agattgatat aaagcctttt tggaggttgc
    11161 taaagtttta aagattaaaa tatatattat aaatataccc gtggtgctag ttaattttaa
    11221 tatcctaaag aatatctcaa agttcct
//
LOCUS       DAFGZE010000092        10760 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.095, whole genome shotgun sequence.
ACCESSION   DAFGZE010000092 DAFGZE010000000
VERSION     DAFGZE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.095"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(59..1042)
                     /gene="istA"
                     /locus_tag="LTU54_002577"
                     /pseudo
     CDS             complement(59..1042)
                     /gene="istA"
                     /locus_tag="LTU54_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="LTU54_002578"
     CDS             complement(1437..2279)
                     /locus_tag="LTU54_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL8297235.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="LTU54_002579"
     CDS             2604..3020
                     /locus_tag="LTU54_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL8297236.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="LTU54_002580"
     CDS             3017..3487
                     /locus_tag="LTU54_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297237.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="LTU54_002581"
     CDS             3501..4274
                     /locus_tag="LTU54_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297238.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="LTU54_002582"
     CDS             4288..5100
                     /locus_tag="LTU54_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8297239.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="LTU54_002583"
     CDS             5154..6119
                     /locus_tag="LTU54_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8297240.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="LTU54_002584"
     CDS             6256..8979
                     /locus_tag="LTU54_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8297241.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="LTU54_002585"
     CDS             8992..9624
                     /locus_tag="LTU54_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8297242.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="LTU54_002586"
     CDS             9829..10056
                     /locus_tag="LTU54_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBL8297243.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="LTU54_002587"
     CDS             10125..10391
                     /locus_tag="LTU54_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL8297244.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="LTU54_002588"
     CDS             10381..10650
                     /locus_tag="LTU54_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL8297245.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3566 a         1716 c         2102 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcgaaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DAFGZE010000093        10551 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.096, whole genome shotgun sequence.
ACCESSION   DAFGZE010000093 DAFGZE010000000
VERSION     DAFGZE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.096"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(40..525)
                     /locus_tag="LTU54_002589"
     CDS             complement(40..525)
                     /locus_tag="LTU54_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HBL8297246.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(527..1963)
                     /locus_tag="LTU54_002590"
     CDS             complement(527..1963)
                     /locus_tag="LTU54_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBL8297247.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(1996..2847)
                     /locus_tag="LTU54_002591"
     CDS             complement(1996..2847)
                     /locus_tag="LTU54_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HBL8297248.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(3003..3248)
                     /locus_tag="LTU54_002592"
     CDS             complement(3003..3248)
                     /locus_tag="LTU54_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297249.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            3353..4126
                     /locus_tag="LTU54_002593"
     CDS             3353..4126
                     /locus_tag="LTU54_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297250.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(4179..4493)
                     /locus_tag="LTU54_002594"
     CDS             complement(4179..4493)
                     /locus_tag="LTU54_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297251.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(6012..6230)
                     /locus_tag="LTU54_002595"
     CDS             complement(6012..6230)
                     /locus_tag="LTU54_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297252.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(6245..8872)
                     /locus_tag="LTU54_002596"
     CDS             complement(6245..8872)
                     /locus_tag="LTU54_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297253.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(8859..9077)
                     /locus_tag="LTU54_002597"
     CDS             complement(8859..9077)
                     /locus_tag="LTU54_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297254.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(9074..9949)
                     /locus_tag="LTU54_002598"
     CDS             complement(9074..9949)
                     /locus_tag="LTU54_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297255.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(10214..10513)
                     /locus_tag="LTU54_002599"
     CDS             complement(10214..10513)
                     /locus_tag="LTU54_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297256.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
BASE COUNT         3176 a         1988 c         1318 g         4069 t
ORIGIN      
        1 aaaataacat gtcattagat agtagacagt tccaactctt tatttacctt ttttgtatgc
       61 tgcactgtca atccaagttc ctaaattgtt gtatccatca tcacttatat aatcatattg
      121 gccatcaata atttcgtcaa accacaaata ttctccatct ggtttttgcc caattatttt
      181 aattactgat tgactagtag ttgtcatctg gttaaaatct tttattttac ctatattaac
      241 ggccaaaata gcatttcctc gcttataact ctcctggatt tcatatttaa catattctcg
      301 atcaagagtt tctttaccta tcagcaccac agtcactgat gttccaatta attgattatc
      361 tatccagttt tttatagcaa tatctccatt acgtttaact ttctcaaatt cagccttatc
      421 tacaaaacca gcttcctttg ttcctcgcaa agtccagctg tttctaacta ccattgctct
      481 tgttacatct gcataatgga aactaaaaaa aacccttctc accatattaa tcctcctgaa
      541 tactatttat taaactgaca atttgttcaa cttttttatc tacttctcct tcagtatcta
      601 atttttctag cgcttgaaat ttacttttta aggaatcatc aaaataatca ctgaaattct
      661 cttgaatctt atcaaacaaa accttacttg cacctttggt aaatccaatc ggaactacta
      721 tattttcatt ttgaacagat aattcaaatt cttcaatcat tccatctgct gtaactattt
      781 cacctgattc aagtttgttt ccaaaaacaa aaatactaat cccagactct gcaataatgt
      841 tttctcgata tcttctccac tcatgaggca tatcaagggg aagtggtctt aaagaaagta
      901 cgtcttgtat gttatttttt cttaaatttc ttctctgctt tagaactcct gaaacaatac
      961 tattaccaat tccaagtccg taacctgata ctattctata accggattca gaaagcctat
     1021 atgctaattt ctcgacaaat tttaatcctt tcgattcaga tgtaaatgga gtataatcaa
     1081 cagcacttcc cgaaataaaa atagtttttc tcaaatactt ttttctaata cttttcaaaa
     1141 tatcagtaat ctcagaataa tcatctactt caacaacgtt tatcgaaaat ctggacaaat
     1201 cattgatttg tagtcttctt tttgtcagtt cataattata tctctcttga tcaaaaaaat
     1261 cctcagaatc gacaaaattt tcctttaata tttttctcat tatacaataa tgaggtcttt
     1321 ggttttctct caaaacccat cttatatctg ataaaattct ttctaaatta gggtctgtaa
     1381 aactaaatcc taaaaagaga aaagtttttg ttaacaaatc tccttttaaa gtttctttaa
     1441 ataacgcgta cgaatcatca tcatattttt cataatcatc tcttgttatt actgtcgaac
     1501 tgacatcatc tatatcacca tgcattttat aaacaattgt atccacattt ggattagttg
     1561 ttgctaactg agtataattt ttcttaacct gagggttttt ataagcttct cggattgatt
     1621 tttctattag atgatcataa ttagttgtcc aataagtttc aataggtaaa cttgctagta
     1681 ttcgatgatt ttcagatatt tctaattctt caccaaaatt atccataagt atctgattca
     1741 aaattcctct tgatccacca ttttcatttt tataatattg agccagagta actaagtctg
     1801 tttctttttt agaatccaac ccgatttctg ttgcaggatc attaagtagt tccggccaac
     1861 ttacatatcc ttgtgacatc gataatcctg cacctgcaaa tattgctgca tttccttttt
     1921 caatagcttc agagaacctc tttattaaaa ctttcttatc cattctaccc ggttcctttc
     1981 gattcagtaa attgattaga gattagtgaa aatggtgttt ttatataaaa tatctaatgt
     2041 attacttcct ttagtgcatt ccacagcatc taaagcccat tttttcaaat cagcttttcg
     2101 taaataagtt aattcactta aatttaaaga atattttatt tgcagatttt cttgcgaaga
     2161 atagtgcttt ataatgcttc taacatcctc ggtgctcatt tgaggattac tttccaatcg
     2221 tggattcttt ttttccacta ttgaagctgt cgtcagttcg ctatatagcc atggcgaata
     2281 cgtaaatttc tgttcattag tgatttcatt gctcaaacta attgaattag gtgtattaag
     2341 aaaaaacaga cattctgtac tatcaatcat attatttaaa gctgtagaaa gcatcatatg
     2401 aacatgacta gttgttacat ttcttttatc ataatcatat gtttttgttt gttccttata
     2461 gcaatatttt tggtctattt tttttaataa atcagaactg tatccccaaa ccgaagaatc
     2521 aataaatgca gtaagaccaa aattttcaaa tagccatgat gcaaaacgta tagctaattt
     2581 ttcatcagca tgagagtgag atataaaaat atctgctttt atagttggaa accaatcttt
     2641 aataatttcg tctgcatcta gaattttgct gtccttagaa taataatcaa ctaaatcatt
     2701 ttcactttca aatagatttt ctattctctt gttaatagat ttttggttgt gatctatttg
     2761 atttactaca tgtgtatatg attgcgagct tgaggagaat gcatttaagt cagcatttgt
     2821 aaaatccaat tcatatgcct tatacaagga tatccctcca ttcttgtatt ttgatccaca
     2881 atttattagg caacataaca gtatacccta tcgttacata tcccattcat tattatacca
     2941 gtatttcttc gtgacacata agggtgtaac ataataattt caaacgaaat gactacaagt
     3001 agtcattgaa gcaaatcttc ataagacacc tgtagaatat ctttgatggc tttcaattga
     3061 tccaacttaa tgtgttggcg gccaccttca atcttaacca acgtttccct agttatagca
     3121 attttacgaa gctgtaggtc tctaatcagc tcggtttgac caattccttt ttctaatcgg
     3181 actcgtctaa tattgctacc gattacattt tctttatcaa taattttttc ctctttcaag
     3241 aatagcacac cttttggact aaaattagtc ctttttctta ttaatcttaa agattcggta
     3301 tgctacaatg ggactagtat tagtccatat tcaattttaa ggaggaattt ttttgaagaa
     3361 atttgcaatc attctaactt gcgctttact catcggaact ataagcgggt gtaccacttc
     3421 tgacacaagt tctacaagta cggaaactag tacaacaacc tcagaaaaga attacccaat
     3481 acattttttt ccggttccag atggatttga agcttattat catgataaaa aactagatgt
     3541 tcctttatca gaaactgaat ctactgaaaa gattttgcca attgaaattg aaagaaatca
     3601 agacgatata aaactaaaag ctgatcttcc cttactgaac agcacacaaa ttgatagaca
     3661 tgaaaatatt aaagttaata ttgaaaaagg agcgttagca caactttata ctgaaaaagt
     3721 tgaacaattt tttgaggctg gtgccaataa agacttttca aaaataagcg attattcgga
     3781 taactttttt aatgagaccc aagatgaaat cacgaactca acttttatgg ctgaagatat
     3841 gtacaatatg aagttgaaaa aaataaagtc gtatgattat ccattaaatc taacattaaa
     3901 taaagggata ctctcttttg tattagcagg ttacgttgac tatgaatata cagcagatta
     3961 tttggagacg ccccttcaac acgataataa cgatattaat tttagattta tctacaattc
     4021 aaaaagtaaa acatggttag ttgatcaatt gagcactata aatacgggat ttcatgaatt
     4081 acctaagcct gacaatgaaa ctgctttggt cattaaaaca ttctaaaaaa gaaacgttga
     4141 aggaataatt attctttcaa cgtttctttt attgtttttc agtttacggt cttacctgag
     4201 cgaataatct gatttctaaa tgattggaaa acttcttgtg gttgataata atctgtatct
     4261 atttcaaatg tttctgagaa taattcattg aatttttcta ctgaaatgat gattttacca
     4321 ttctcctctc gactgttttc tattatttct gcctctaatt caaataaatc tagtgacgaa
     4381 ctaaaatcat aaatcctgtc atattgaaca aaaaaatcaa gaaaaagttg ctcaaactct
     4441 tccttattta tcactagtgt ttcaagcgga acaactttat tttcatcagc cattcttcca
     4501 atttcctcac tttcattttc aattgtccat tgttgcggac gtatcagatt ttctctatca
     4561 tttttcagtg tatagatcac ttgtaataca caacaatgct ttgtaaactt atttttttag
     4621 ttagtcgaat ctttttaaat gtgcgtttca taccatgcgc ttgatatatt ttctcctatt
     4681 ttttaaatcg tttatcacaa cttttatcgt tttttcttct gaaaaaaatc ttgtttacat
     4741 cgttacatag tttccaaaaa gatacattac tagtagaaac aacaagatta attttacttc
     4801 ccactgaaat gggaagtata ttttaaatat tttttctcct tcaacttaca ctataaagta
     4861 tcttaatcac acgatatgct agccactaaa ttcatcatat ttgattcctt ttttaagata
     4921 atgtagcatc tttttatcca ataacgcaat tgtttttaag gataaatttc ttaagatggt
     4981 acatttcaaa atggcatttg acatatgaaa caaaaaagaa agaaaagagc ataagggaaa
     5041 actgatagca ccttcctaca ggaaggtggc taagttggct gtgccaactg gttttctttc
     5101 aaaatcattt cataattttt gatatcataa aaaaatccat tttggaccta ttttcggtca
     5161 taatttagta cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaagg
     5221 cttttctgtt cacaaaattg gtttaaattt gctttcttta acacttcatt tatatcatag
     5281 caattataat gtttacacaa catcattcct aattttaacg gttgttcctc tttatatttt
     5341 ttaagttcta catttttttg cgtttaatta cttttctctt ttctttatat tccgctccaa
     5401 ctttctcttt aatagtgata atagaattac atatttttgt taaacttcct taaaaaaatt
     5461 ggatctcgat tactcaaatt tgcttgcagg cttttctaat tgctttgttt tttcttccta
     5521 attatttgat tttatttcca ttttcttcta gtgaaaattt attattttta ataatcgtac
     5581 gcgtagaact gatttcattt tttgtatctg tttgattatc taacgtttga aattcaaagt
     5641 tttgattggc tttatcaata tctttttata ctactttatt tgcaagatat attttcttgc
     5701 ttaatttggt catagtttca ttcgtcactt cggtgatatt tttttaattt gcaaataaaa
     5761 agagttaaac cgatatacat taggttcaac tcttttgcag gtttcatggg tgtactatca
     5821 cttttaatct gatagatgta aacctctctt tatctttttg ttacttcaat tttcaacaat
     5881 agctccttta taggttgtca atattgctct acgtattctg gctttttaac ccattatttc
     5941 tctaaagtgg aagagaataa tcgcttaatc cccactaatt ccttgtcgta aattcatgtc
     6001 aaatacaatt tttaaaagcc atttaattca tggattatct cttcactttt ttctagataa
     6061 caatatttca agttgttttt tatctcatgt ttcatcttta attcttcttg aatcatcagt
     6121 tcaatataat tagtgaccgt gattttttca tttgttgcgt tctttactga ttcacataat
     6181 tcgtcaagtt ctaagaaatc atgcgtacta atctttagaa ttaatttcat gacaattccc
     6241 ccttttattt ttttaataga ttatcgaaaa gcggattagg atcattaagt gtatctctga
     6301 ataactcgtt gtcttcatag gtaaatcttc gtcttggttt tgatccctta tcattttcac
     6361 ggatttcact atcaataatc actgtttcat gatttgaatc aaattttttc gctgactttc
     6421 tgtcttcaac gatattgatt ttttgtgttt tttctttcat ttctccacta tctttttgaa
     6481 cgttctcccg aacatctact gttttgaatc cttgactact tgtagaactt tgtacattct
     6541 ctcttacatc cattgattta aacccttgac tacttgtaga attttgtaca ttctctctta
     6601 catccattga tttaaaccct tgactacttg tagaattttg tacattttct cgaacatcaa
     6661 ttgtcttagt accttgacca tttgaagagc tttgtaaatt ctctcttaca tcaacattcc
     6721 gtccgacatg accattcaat gaaccttgaa tattttctcg gacatctact gatcggccac
     6781 cttgactact tgtagaattt tgtagatttt ctttgaccaa tttttctaat ccggtatttg
     6841 agtttttagc ttcttggatt ctttctttgg tcaacgcttc tttttcgtcc gtattagctc
     6901 ctgtacgccc ttctacacgt ttccttaaca tttcctcacg attcattgct tcaggcgtgt
     6961 atttagcttc ttgtcgcttc tgattaatta attcttcagc agacttagct tgaccgtgca
     7021 tagcattttt acgttcttca atatcttgtt gaactttttg ctgaattgca cttacaggat
     7081 tattattcga ttcagcatta tttttcattg cttcacgcat acgttcagaa ctagacataa
     7141 ttctatcggc attttcaacc ttagcaccat cttttctatc cgaaagacgt gttggcatgg
     7201 aatcagcata ttttccagtt tcatcttctt tatttttagc taacataggt ttgctatttt
     7261 tattaatagc ggacactgta ccatcatcta aaccttcttt gaacttattc ttggtatcat
     7321 tagccatatc acgcatactc ttaaatggtt ttattgctct ttcactagcc attgtagctc
     7381 catctgaagc taaacctgat aatccacgtt catgggcata tcccaaagct cttccagttt
     7441 tgccagcttt ttttgataga gtttcgccga aattcttttc tggttttcga tcttctcctt
     7501 ctccactgtc tgatccattt ccatttccgc ttcctttagc acgattaaat ccagttgatc
     7561 cttttctaaa cagcattgcg cccataccaa aagcagaagc aagttgccca tagccttctt
     7621 tgagtccaat atcaacacca aagtatctca tgactgttcc actgcctttt atcagcacaa
     7681 acacagcaca gatataagca attgttttta taaaaatatt tgtggagata ccttgtccag
     7741 catttaaaaa attgataaac atagcaaaca tagatagtcc aaacccttga aatccaactg
     7801 ttaagtaaca ttgtaaaata tcggataaaa caacttttga acgttgtcct gtttcaatat
     7861 ctgtcgcaaa aacaagaaca cctaacacac gtttaaatgc aagttctaat attgctgtaa
     7921 tgatcacaaa cgcgctgaac acataggcga ttgccaacgc tgtcaaccca atcgaaatcc
     7981 cccaaagatt tgcttgatat cgataataac ctgatttcaa gctatcagaa aatgttgata
     8041 agaagttatc agaaaacttt gtggcaacaa attcattatt agaattttca actaattcat
     8101 agcgaagatt atccgcagct ggattttctt tttccaattt atcaatcact ttatccgtca
     8161 acacttgtgt taaatctgtc tgttgaaaat tttttcgtgt taacttattt cgatcctccg
     8221 ttttatcaat gcttgagtat tgatctgttt tattaatata tgccagatct gttactcctt
     8281 gttttatcaa tgaccaagcg actccgtcat ctgaatttgt aatcgtagta gcatcattgt
     8341 aaaatatttt ggcaaattga ataccgctag aaatcattgt tggcattaaa agaattaacg
     8401 ctacagacat cactatattc attccaacag atcttaaatc tattgttcct tttccagtaa
     8461 tcatcttgta gccaacaaac atcaatgaaa ggatcattaa tccaacgaca attgtattta
     8521 tgactgattg ataaacatta tttagccctg tactttcaat gaaattgaac aaatccaaca
     8581 catctggaat catattttga atcgaatcag tcacagtaaa catgcctttt accacagccc
     8641 atccaagcca aatcccgaac tgactaagga aatctccatt gtattttttt aggtaaggtc
     8701 tataaagaga attattcaaa aactctgttt gactttttgt gtcatcaaac caattgaaaa
     8761 tgctagcttc aacaatatta cttgtaaaaa caacgctaat cattaaaaca agtacactga
     8821 gtgctaacca gtttattttt ctactaacga ttttttcttt aattgctttc atagttttct
     8881 ccttcagccc attttcttag gctctcagag agctgctgag ccattttttg attaattatg
     8941 atatgagtta cacaattgtc attttcagct cttaaatcga gaactactgt gttgaagcag
     9001 atactctcga ttaattcgag agtatctgta ttttgatcaa gacaccacat ttgatctatt
     9061 aagcgatttt taatcatgat tcttctcctt tcaaccaatt atgcatagta atttcaggta
     9121 actctttgat ttcgaaagct tttttgaact gatctatagg ctctacattt tctaaaaatt
     9181 gacttttata tcttttctga agtccattca aaaaatatgt tacataatcg tataaccctt
     9241 cttcagtttt tctttgaaaa aagcatgctt catgttgttt tataatacac tcagcaataa
     9301 gatactgagt attttcagat aaatattcaa tgatttttct gatatcttct gtatctacat
     9361 tagcgtttct caaatagtga tacacttgaa ataattttcg accatcttcg ttatttgcgt
     9421 cataaagaac actaatctta ctgtaaatcc tctttatatt aatatcctct tcttccataa
     9481 cgagagtagt ctttgatatg ttctgtgaag tagtctttgt atacgtatat acaaatttgt
     9541 acatacctct accttttttt aaagagacat atctcttttt ttcgataccc ctctgacgaa
     9601 ttttcggcat atactccttt ttttcgcaat ccaaagtagg aaaagtaatt tctatcaatt
     9661 tatgtacatt taaaacaatg tacataacat ttgataatgt aactgctctt ttctcataat
     9721 aaatatttct aaaaaccctt ttgataatcc cctttttctc taagtacaca attgcatctt
     9781 ttatagctcg ttctgataat cctgtttttt tgcttaattg cttatatgat ttttgcaaaa
     9841 tatcttcaag aaactttttt tgataaccaa gtagctgacc atcatcgtta aaaatttttt
     9901 taggtctata ccaataggta atttctgcta agataactat tgcattcaaa tagggcttct
     9961 caacaccttt tttcgttttt tttcttaatt gatcgtacca tgtgtgtgga ttacatttcc
    10021 gtcaaaattt atttctctaa gtttgtcaac tacaacattt ccagaagaaa acaccttttc
    10081 accttctttt ttgatataat agctataaat aaagttttct cagcacttta ttattgagtg
    10141 ttcccccagt ctgcaaactt ttgggaacac tttttttctt tttttaaagc atttttttat
    10201 aatttctact tcatcatctt ccaatatttt cccctctttt cattcgagca aacataaact
    10261 cattaactgc ctttttcgaa taaaattttc ttgcccctaa ttcaactacg ggcaatccct
    10321 catttctcac agcatttttt aaaaagtttt cactcacatt taagtattcc gccatttctt
    10381 tttgattgag atattccctt gtcatccccg cattttcttg agccttttca atagcttttg
    10441 tagcaatttg ataaaactgc tttagaaaca tttcctcctg ttcttctgaa atcattaatt
    10501 ttattgcttc cacaaaaacc tctccttttt aattaattat tctataactc c
//
LOCUS       DAFGZE010000094        10254 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.097, whole genome shotgun sequence.
ACCESSION   DAFGZE010000094 DAFGZE010000000
VERSION     DAFGZE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.097"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            158..307
                     /locus_tag="LTU54_002600"
     CDS             158..307
                     /locus_tag="LTU54_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HBL8297257.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            318..1832
                     /gene="dltA"
                     /locus_tag="LTU54_002601"
     CDS             318..1832
                     /gene="dltA"
                     /locus_tag="LTU54_002601"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HBL8297258.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1829..3034
                     /gene="dltB"
                     /locus_tag="LTU54_002602"
     CDS             1829..3034
                     /gene="dltB"
                     /locus_tag="LTU54_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBL8297259.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3079..3312
                     /gene="dltC"
                     /locus_tag="LTU54_002603"
     CDS             3079..3312
                     /gene="dltC"
                     /locus_tag="LTU54_002603"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBL8297260.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3315..4583
                     /gene="dltD"
                     /locus_tag="LTU54_002604"
     CDS             3315..4583
                     /gene="dltD"
                     /locus_tag="LTU54_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBL8297261.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4724..5806
                     /locus_tag="LTU54_002605"
     CDS             4724..5806
                     /locus_tag="LTU54_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBL8297262.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5806..6609
                     /locus_tag="LTU54_002606"
     CDS             5806..6609
                     /locus_tag="LTU54_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBL8297263.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6679..7419
                     /locus_tag="LTU54_002607"
     CDS             6679..7419
                     /locus_tag="LTU54_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBL8297264.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7430..8434
                     /locus_tag="LTU54_002608"
     CDS             7430..8434
                     /locus_tag="LTU54_002608"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBL8297265.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8479..10176)
                     /locus_tag="LTU54_002609"
     CDS             complement(8479..10176)
                     /locus_tag="LTU54_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297266.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVKDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3296 a         1696 c         2078 g         3184 t
ORIGIN      
        1 aaattataaa aaaaaaagca gtgatttgat gtgctatttt tataaaagct atgtgacaaa
       61 actgttagta aaaaaacggt ttctgtaagc tagacacatg tttctttttg tcaaaattag
      121 ttacactttg tgtgaggtta tatgaggagg gaatccaatg aacaaatggc gttctaatcc
      181 aaaattgaat tattggggaa aattcatcat tcgaacaatt ttttatagtg cagtactgct
      241 gttcctgatt tatctgtatc actataaaaa tattcaagga ggaactttta tctataacga
      301 attttaggag gaagagtatg gaaatcaaaa cgattattga agcaatcgat gattggggaa
      361 taaatgaacc tgatcgagtt gcttatcagg cggatgagac gcatactttt ggtgaattga
      421 aacaagcatc agacgcactt gcttattatt tagaaaaaaa ggtagaaggc gatggaccaa
      481 ttgtcgtttt tggcaatttg gagtttgaaa tgattgtctc atttctaggg gttgtcaaat
      541 ctggccatgc ctatttgccg attgaagagc atacacccaa ggagcgtatc ctatcgatct
      601 ttcgggtggc taaaccatct atggtcatca gtatcgggga ctggatagaa gatattcctt
      661 cggttcctgt cataacaaaa aaagaatttc aagaaatcaa gaagatacca gtaggatttt
      721 atcccagaaa tagcgtaaaa ggtacagaaa atttttatat tatttttact tctggtacta
      781 ctggagaacc aaaaggcgta caaatcagtc atgataatct agtcagcttt gttcaatgga
      841 cgatggaaga ctttgggatc aagcctggta tgcatttctt agcccaagcg ccattttctt
      901 ttgatttatc ggtattttct atttatcctg cattagtttc tggcggaatg ctgaagccgt
      961 taaataaagc agttgttcaa gactttcgtc aattattcgc gacattgcca gccttgaagt
     1021 tgaatgtgtg ggtttctact ccatcattta tggatatttg cttgatggaa cctacattca
     1081 atggagaaaa tgtgccggaa ttggacatgt ttttattttg cggagaagaa ttgacgaaaa
     1141 aaactgcaga aagtctactt gatcgattcc caaatgcacg gatatataat acttacggtc
     1201 caacagaagc aactgtagct atttcaagta ttcagattga ccagcatgta ctagatagtt
     1261 atgaccgact accgattggc tacgtaaaaa aagacacgaa agtttctatt gtccaagaag
     1321 gtaaacctgt ttcaaaagga gaaacgggtg aaatcattat tgcaggacca agcgtatcaa
     1381 aaggttacct gaacaatccc gaaaagacag ctgctgcgtt cttttgtcaa aatgacttag
     1441 caagttacca tacaggggat gcaggtagac tagatgaata tgggctttta ttttatgaag
     1501 gacgtatgga tcagcaggtc aaattacatg gataccggat tgaactcggg gatatcgaac
     1561 attatctgct ccaagacaac cgaatcaaac aagctgtcgt cgtaccaaaa taccaaggaa
     1621 caaaagtaca gcagctggtt gcttttgtcg tattagaaaa caaaacgaaa cagccggatt
     1681 ttcagttgac taaatctatc aaagaacaat tactagaagt tgtaatggat tatatgattc
     1741 ctcagaagtt ccattatgtc gatctgctgc ctcagacagt taatggaaaa atcgatcgaa
     1801 aaaaattaac agctgaggtg aatcctgaat gatgttacct catatgatcc cttatgcaga
     1861 gccgttttat ttttttcttt tagccattgc gcttgtaccg atcgttctat ctttattgat
     1921 atgggaaaaa agattaccag tttatcaatc agcagtgaca cttttctttt tatttgtcag
     1981 ctttggaggt aattattggc aacagggagc ggcgctgatt gggtatgttt tgtggcagac
     2041 gatacttgta tggggctatt ttgtttatcg gcaaaggaaa aatcagacag gatggttttt
     2101 tgccgccgtt attttagcga taattccact gtttttaacg aaagtgactc ctttttttga
     2161 acatggaaaa gcatcgttac ttggtttttt aggaatctct tatttaacct ttaaatctgt
     2221 acaaatgatc atggaaataa gagatggcat gatcaaaaca tataagccat ttcgttatat
     2281 ccagtttctg ctgttctttc caacaatctc ttctggacca atcgatcgct ataggagatt
     2341 tgaaaaagat cagctccatc cgcctgttcg tgataaatat gttgatcttt tgggaaaagg
     2401 cgtccaaaat atttttgtcg gttttttata taaattcatt atcggttatt attttggtca
     2461 agtactgctt cctgtagtgg gacgcattgc cttacagcat ggtggtattt catgggcatt
     2521 agtaggttat atgtacgtgt acagtatgta tttgtttttt gattttgctg gctatagctt
     2581 atttgcagtc ggtacaagct acatgatggg atatcagaca ccaatcaatt tcaataaacc
     2641 ttttttatca tggaatatca aagaattctg gaatcgttgg catatgacgc tgtccttttg
     2701 gttccgtgat tatgtatata tgcgattgat gttcttcatg atgaagaaaa aattttttaa
     2761 aagcaagatc gtaatgtcaa atatcggtta ttttgctttg tttcttctaa tgggtatatg
     2821 gcatggattg acgtggtact acatcgtata tggactgtat catgcttgtc tgatctgtct
     2881 gacagatgca tggatccgat ttaagaagaa gcataaagag cagttgcctt ctaacaaatt
     2941 tacgcatggc ttagccgtat ttttgacatt tcaagcagta tgttttagct tcctgatttt
     3001 ttcaggattt ttagataaac ttttttttag ctaacgaata gtgacatatt aatttatata
     3061 acaaaggaga aaaggaaaat ggaagaacaa attttatcaa tcttagaaga aataacagga
     3121 acagatgaag taagaaaaaa tcgtgatatt gatctatttg atgaagggtt gcttgattct
     3181 ttagcatctg ttcaattatt agtcgagcta gacggagagc tggatatcca agtccctgta
     3241 tctgaattcg agcgagagga ttggagcaca ccgaataaaa tcattcaaca agcaactgca
     3301 ttgaagggtt gagtatgaaa aagaaacttt ttgctatttt cggcccggtt gttttagctg
     3361 ttggcttact gtttgtgttt tttacctcct cctatcaagt agatatctct aatccagaaa
     3421 cgattagacg tgcctcttct tccatgtcag tgaacgtgtt gaaaggagat gcgataaaaa
     3481 attatgcatt atctgaaaaa caatatattc ctttttttgg ctcaagtgaa ttaagcagaa
     3541 tcagtccatt tcatccgtct gttctggcag aaaaatacca gcgaaactat cgtccatttt
     3601 tgctgggagc acccgggaca caatcgttaa gccaatatat gatgatgcgt tctgcaggag
     3661 atgctatgaa aaacaagaaa gtcgtcttta ttatttctcc tcagtggttc gttaaaaatg
     3721 gtgtaaaaac tgattacttt aatacgtatt attctgaact tcaaacatac gactggttgt
     3781 tttcaatgaa gaaagtaacg cctgctgatc gctatttagc tagaagatta ttaactttct
     3841 caaaagtaaa agagaatgat acgttgacgg cgattctgca aacgatcaaa aaagggaagc
     3901 tacctttacc tgaatctttg aatcagctgc gcagccaatg gaacatgctg aagagagagg
     3961 atgaagtatt cagcaatatt gggttgaaag atcgacagca aaaaatcgat catgagtcaa
     4021 aacgattacc aaaacaatac caagaaacag aattatccat tttagcaaat caaattggag
     4081 aacgagaaac gacgaataat ccattcgggt tgaaaaacga tttttatacg catcgtatcc
     4141 gagcccacga gccagagctc aaacagtcac aaaaaaattg ggattatcgt ttttcgccag
     4201 aattttctga ttttcagctg gtcttggatc aattagcaaa aaaccataac gaagtattgt
     4261 tcatcatccc accggttaac gagaaatgga gcgattatac cggactatct caagaaatgt
     4321 tacaaggatt tgccaaaaag atcaaatttc aattaaatag ccaaggattc aatcgaatag
     4381 ctgattttgt caatcaagcg ggtaccaact attttatgga agacacgatc catctaggct
     4441 ggaaaggctg gttggctgca gatcaacaga tcagaccgtt tttagaagaa aatcatatta
     4501 ctgctagtaa gtatcatctt gacgatgctt tttttagcaa atcttggcaa catcaaatac
     4561 cagataaact acaattaaaa taaagaaaac aggcgagtgt tgctcgcttg ttttctttat
     4621 ttttgaatat tctgaataaa agataaaaat accaagtttg cctgaacaaa aatcataagc
     4681 aatggtataa tcacaatatg aatatagaaa tgggggaaaa catttggata caaaagaaga
     4741 acagctactt attgatttaa cttctgcaaa aggagtacct ggaaatgaag aagaggtaag
     4801 agaagtattc agagaatatg ccaaaccatt cgctgatgat attttttatg acggtttagg
     4861 cagtgtgatt gccaaacatg gtgctggcgg aggccccaag gtctttattt ctggacatat
     4921 ggacgaagtt ggttttatgg taacaaaaat aaccgaaaaa ggattcttgg aattccaaac
     4981 tcttggaggt tggtgggggc aagtcatgct tgctcagcaa gttgaaatca aaacacgtga
     5041 agggaaaatc gtccacggtg tgattggttc caaaccacca catgttttaa cacctcaagt
     5101 aagaaataaa ccttatgaaa taaaggatat gttcattgat attggcgcat caagccaaga
     5161 agaagccaaa gagtggggca tacgtcctgg agatatggtc actccatata ttgaatacaa
     5221 acgaatgaat ggcagtaaat atttactggc aaaagcatgg gataatcgta tcgggacagc
     5281 tgtttctctg cgagtattag agaatctatc taaagaagca cacccaaatg ttttattcgc
     5341 aggaagcgat gtgcaagaag aagtaggctt gcgaggtgct cgaacaagta cacaccttgt
     5401 aaatcccgat attgcttttg ctttagatac tggtacagca ggagacactc caggaatgac
     5461 accaaaagaa gcagattcga ttttaggaaa aggcccacag attttgattt ttgatgcatc
     5521 tatgatcccg cataaaaaat tattgaattt tgttattgat gtagcagagg aaatggatat
     5581 tccattccaa tatacagtga tttctggcgg aggaactgat gcaggacaaa tgcatttgac
     5641 aagagatggt gttccgtctg tagccattac cgttcctgtt cgttatctgc attcccatac
     5701 atcagtgatt catgaagatg attatttgaa tacagtgaaa ctagtcacag aattagtgaa
     5761 acggcttgat agtgaaacag ttgaaaaaat tcggagttat gaataatgaa taatagaata
     5821 cctatttctg aatatcggaa aaaagcacga gtttctttgg aaaatcaatg gggaatcaat
     5881 acttggcttg ttttcttatc tataatattt acgagcgtca tccagaactt ttttgacggt
     5941 ttcagtcctt ttccggaaga aagcatgcag cagcacttat tgagttttct agtcgataat
     6001 ttacttttat tcgcattcaa ctatgcattg ttttacattg cgctagtagt tgtacggggt
     6061 ggcagaccaa aaagtaacct tttattttca gttttccaaa gaaagtattt tggttcgata
     6121 cttggcatca atttgatcaa ttcagtcatc aactggctac tcaacagttt aatctttctg
     6181 cccatgtttt taatcagcgg agtgagtact tatatgcagc tgcttttcag ttcaaatgcg
     6241 gtacctacgg gtgtcatgcc agaagatttt cttgatatca gctttgtttt atcgatgata
     6301 gggatgattt ttgtttcagc actcatcatg agtattgttg gcggtttatt ccaatttgct
     6361 gcatggacaa agttcgacta tcctgatttg tcggttattc aatgtttgaa atatggctgg
     6421 tttttattga aagatagaat tggtacgtat attttgttgc agctgtcttt tatcggctgg
     6481 tatttgctag gagcaatcgc tttgttcatc ggtcttttgt gggtcattgt ttatgtgaat
     6541 gtgtcagttg cagaatttta tgaacaagca cggattgaaa aaggaggtcc agacgcctat
     6601 tttacttagc atgtaagcag tttattcatc aaaaaaataa cttttgtggt aggataaatc
     6661 tttgaggggg aatgtacgat gaaattaact gttttaggat gcttaggagc gtatccttat
     6721 aaaaatcaag ggacaacagg ttatttgctt caatctgacg ggttcaattt gttgatcgat
     6781 gcaggtagtg ctacgctgat caaattacaa gaacaccttg atccacttga tctagatgct
     6841 gtgatcctta gccattatca tcatgatcat attgctgacc ttggtgtatt acagtattac
     6901 tggcaacttc atcctaagag aaatccggag acattgcttt caatttatgg acacacaaaa
     6961 gattccattc actttgaaga attgacgttg gaaaatgtct caaaaggaca tccttatttt
     7021 gaagctgaag aattaaaaat tggtcctttt ctgattactt ttatggaaac aatccatcca
     7081 gtagtatgtt acgccatgcg tatcgtagaa gaaacaacgg ggaaagtatt cgtatttaca
     7141 ggtgattcag gttatttgga aagtttcgct gattttgcga aagatgctga tttgtttcta
     7201 gcagatacct atctttttga aggaaatgaa catcatcatg cccatttcac ttcaaaagaa
     7261 gctggagaac ttgcgaaatc agctaacgta aagaaacttg tacttacaca cctacctcag
     7321 tttggtgatt tagaacaatt gaaaaatgaa gcgcaaaagg cagcaggcaa agggattgat
     7381 atagaacttg ctgcagttga caaagtgttt aacatttagg aggaagaaca tgatttttgt
     7441 accaaatgaa aaccacgacc caagaatcaa tttagcaatt gaacaatatt tgctgcaaga
     7501 aatgccttta gatgaaccta ttttgctctt ttatatcaat gaaccttcaa tcatcatcgg
     7561 cagaaaccaa aatacgcttg aagaaatcaa tcgagattat gtggaagaaa atggcattca
     7621 tgttgtccgt cgcttaagtg gaggcggagc tgtctaccat gattttggaa acctgaactt
     7681 tagttttatc atgcctgatg atggggattc attccgtgac tttgcgaaag taacacagcc
     7741 aatcatccaa gcacttcatg aattgggtgt tgctggggcg gaattgaaag gccgaaatga
     7801 tctagtcatt gatggcatga aattttctgg caacgcgatg tatgctacca acggtcggat
     7861 gtttgctcat ggaacaatta tgttcgactc tgatatcaat gaagtagtca atgcattaaa
     7921 agtaaaaaaa gacaaaatcg aatcaaaagg aatcaaatcg atccgttctc gtgtaacgaa
     7981 tatcaaacca tttcttccag aagaaaagca aaaaatgaca acagaagaat ttcgtcaaga
     8041 aatcttgttg aaaattttcg gcgtagagtc agtagaagaa gtaaaaacgt acgaattgac
     8101 tgatgaagat tggaaaaaaa tcaatcgaat ctctgaggaa tattatcgaa attgggattg
     8161 gaattacggg aagtctccag atttcaattt ctctcgccaa aaacgttttt ctatcggttc
     8221 gattgaagtc catttgaatg tctcagaagg aatgatcaaa gaagcaaaaa tctttggcga
     8281 tttcttcgga ctaggagata tctctgacgt ggaaaaaaaa ttggttggac agaaatatga
     8341 taaagcatct ctatcaaaaa tagtcagcca aattgatgtg aagaaatatt ttggtgcgat
     8401 agaaccagaa gaattgctta ccttacttta ttgaaaataa aaaatagcgg aatccacata
     8461 tatggattcc gctatttttt atacaggatt cgggtaattt cgagagagtt gaacagtgat
     8521 ccgatcaatg aaagtatctt tttcctgcgt tagtgttcct gtgtaacgat ccgctacgtc
     8581 tttcatcatc attaagtcaa aacgttcctg atcatctaat tcttctgtga tgcctcgact
     8641 aaataggttt gtatcgtaat gtgcactatg ttcgcaacaa atgacgaaat gttcctcttg
     8701 ttcgtgtatc gacaaataaa tttttcgata cagtggattt tcgatggcat gagtttctct
     8761 aaaggaatgc tctaaagcgt ggatcaaaag ttgagccaga tcgtcatcgt tgataggaag
     8821 ttcttcggga agatctgctt ggaaggttat ctgaatattc cgtttggcag ctaacttctg
     8881 gtaacgcgct aagataagat ttgtcacctg gtggttgcaa aaagaacaca gcatttcgaa
     8941 attttttgct tcttctaata tcttttccac atagttttta gcttttgttt ggtccgtatg
     9001 cattaaatct tgcagattct ccatctcttg gacaaattcc aggcgaagtg tatttacttc
     9061 ttggatatgc tgtcgaagct gctcataatg ttgctccaac aaatcatttt tgatttttaa
     9121 gaaatctact tgtctccgat ctttgtcggt atttaagatg tgatcgatta gattgtagct
     9181 gataataacg attagaatga taatatataa aatcgtagac caattgatcg ttgtttgata
     9241 gatacctaat gccgtttgga tgtaaatcaa gcaatggata atggctacca agacgatcca
     9301 aggtgtacaa gctacataaa aacggttatt cttatatgct tcgccaatgc atgcaagtga
     9361 agtgactaaa gtgcttataa cactagcaat attagcaatc ggcattaatt caggaagttc
     9421 tgcaggagta aaagttgcat aaagcagaac gccgataaaa atcaaaagtt ggaacgtaca
     9481 aaaaattccg taccattttt tatagtaaaa cattcggctc caatagtagc agatgatgaa
     9541 agctgacgtt aagataataa ataaatgaga taaaaaagaa tgaacgaatg gttcaaaaag
     9601 aataccactt aaaatatctt ctgatacagc ttcaaatcct aatgccaaag caaagcaact
     9661 taagagaata agtgagtatt caagacgttt ttgtttatac atcaagtaga tcgtatagaa
     9721 aaaggtccct atagcgataa aaacagcgat tatcatcgtc aatacttgcg gcactgattg
     9781 tgaataaata aaactaatca atggtccagt tgttcctaga taaactttcg gtggcagtcc
     9841 agcatagttc tgataaggag atgagactgt aagggtcaat ttttttccga tataatttgc
     9901 tggtaaatct ttcacagcca atgagagtcc gggattgctt tcttcctgat aatctttccg
     9961 ttggtataaa atcttttcgc ctagatatac agtaatccat tgatggtttc ctttgattag
    10021 taaatttgct tcgtccaacg gtttttcaag tgtcgtttcc ataatgaatg tttcgtcttt
    10081 atccacggta ggaatcgtac gagtgtactt gctgtcaaac ttaaaatcag gatcatcttt
    10141 tgtatagaaa acccacgaat cttttatttc tttcatttga ttgctgtccg caggagaaaa
    10201 aataaaataa aagaaataag caattcctgt taaaaataac aatgataata taat
//
LOCUS       DAFGZE010000095        10035 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.098, whole genome shotgun sequence.
ACCESSION   DAFGZE010000095 DAFGZE010000000
VERSION     DAFGZE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.098"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(501..725)
                     /locus_tag="LTU54_002610"
     CDS             complement(501..725)
                     /locus_tag="LTU54_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297267.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            916..3693
                     /locus_tag="LTU54_002611"
     CDS             916..3693
                     /locus_tag="LTU54_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8297268.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3774..4193
                     /locus_tag="LTU54_002612"
     CDS             3774..4193
                     /locus_tag="LTU54_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBL8297269.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4205..4675
                     /locus_tag="LTU54_002613"
     CDS             4205..4675
                     /locus_tag="LTU54_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297270.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4692..5471
                     /locus_tag="LTU54_002614"
     CDS             4692..5471
                     /locus_tag="LTU54_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297271.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5458..6279
                     /locus_tag="LTU54_002615"
     CDS             5458..6279
                     /locus_tag="LTU54_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8297272.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6296..7345
                     /locus_tag="LTU54_002616"
     CDS             6296..7345
                     /locus_tag="LTU54_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8297273.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7358..8362
                     /locus_tag="LTU54_002617"
     CDS             7358..8362
                     /locus_tag="LTU54_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8297274.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            8995..10014
                     /locus_tag="LTU54_002618"
     CDS             8995..10014
                     /locus_tag="LTU54_002618"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBL8297275.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3364 a         1653 c         2093 g         2925 t
ORIGIN      
        1 tatttacttc ttttttaatt tgttcttgca atcttttttg ctacgtaatt tttgtaaaaa
       61 agtgggttga gagaaatcta attgattttc aaagaaggag tatgaaaatg agaaaaatat
      121 ttattcgttt attgtgggta ctatcttaga ggaataaata atagtggaga aaactatggt
      181 ttggcgcaga ttctatcagg tttgatggaa cgaagacaac ctcccgacat caagtcaaaa
      241 gctacttgag attttgatct gaaaataaaa aaagaaaaca aggtaggaac atcaatgttt
      301 ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg gtgtacgtaa tcaataaaaa
      361 ggatggtgac aacagtacct ctttactctc tgaaagcatc ataactcgtt tttcctcatt
      421 atgccaataa attgttgtta taccacatat aaggatttgt aatagctaaa aacagacagg
      481 aaagttgaaa tctgtgaatc ttattttgta tggaaggatt tatagaacgc aaaaatcaag
      541 ataaagatga ataaaaatcc tgtttcggct atatattgcg tatctacgcc aataaaacga
      601 gtttgttctg tcatagccat ataaagcaaa ggcaaaaata caactacagc gagataagat
      661 ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc tgtttttata tttatccgat
      721 ttcattgaat agtcatagcc tccttattct ttcatatgtt gaaattccag tataaaaact
      781 ggaaggaact gtacgttttt tagaataaaa attataaggg ataagtaggc tgctgtctat
      841 aataagatat atttttcaat tctttattaa agattttatt gacaaaatga atccgttttc
      901 tatgatggag gaggggtgaa taagttgttg aagaaggaaa ttttatgtta cttagagaac
      961 cagacagcct ttttcgattt agaacatatg aacgaggtct ttacagcaca aaggttagct
     1021 gatcattttg gggtaaaaag aaacacagtc agtcattatt tgaaccagtt aaatgatgag
     1081 caactactag tcaaaatcaa ttctcgacca gtgatttatt tccacaaaga agcttttgaa
     1141 aaacaatttt ttaagttacg cacgaatttt tatttttcta tcgatgatct caaagaggaa
     1201 cagccttttt tcgcgcaacc aaaagactta ttctctttta tgatcggata cgacgccagt
     1261 ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact atccagatgg cggattacca
     1321 cttttgatta caggcgaaag tggcacaggt aaaagttatt tagttaattt ggtctatcag
     1381 tattgtctgc ttcatgatct gctagaagat tctgcaccat tcatcacttt taattgtgca
     1441 caatatgcag ataatcctga acttttgact agcaatttgt ttggacatgt taagggagcg
     1501 tacactggtg cagaagaaaa taaaaaaggg gcttttgaag cagcagatgg tgggatcttg
     1561 tttttagacg aggtacatcg cttaacacca gaaggtcagg aaaaactgtt cacctatctt
     1621 gatcaaggtg tgatttatcg tatgggagaa cctaatttgt cacgccggat caaaacacgt
     1681 ctcttttttg ctacgactga agagattact agtcatttct taacaacttt cattcgaaga
     1741 atacctatcc gaatcgaact acctgcactt aatcagcgta gtcgtaatga acggctagaa
     1801 ctgatttact ctttttttct cgaagaacaa agaaaaatgg ggagcccttt gtcagtcagt
     1861 ggaccagctt tgtcactttt aacaagtcag aaattatccg gaaacatagg agagctgaaa
     1921 aatatcgtca aagtttcagc agcaaaagca tatgctgaac agcgtgaaca gcctgaaatc
     1981 catgtcacga tccatcattt gcaaaaagaa ctacttgcgc agcctattac taaaaataca
     2041 gcacagcaag gaatctatat tacgcaagac cagaccttag agcaattaat ggagaagcaa
     2101 cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa tcctaattga tttcaagaaa
     2161 aataactgtc tgctatattc ttgcgaagga aaattaaagc aagaagtgat tcaattattt
     2221 gattttttgc tgtttgagac cagtagacaa gaaaaacacg agctgctggt ttattggaca
     2281 caatctatcc gagagacctt tcgacaaatg gaaactgcct atcaaatcaa attcgatgga
     2341 aacagtgtct atgccttgag ttactatctc tatcagcgaa gcactatcaa atggttaccg
     2401 gaagaacatg atattatcca actaatcaaa gaattagaac aacaagtttc tcagtcttat
     2461 ccatctagtt atcactatgt ccaacgaatt ctggaattaa gtaagatcaa attagatatt
     2521 gaagacacaa gtatggatcg gattttactg acgatctatc taaaaaaagc gaaatggtca
     2581 aaagaaaaac gactgccaaa agcgatgatt gctgctcacg gttatgcaac tgctagcagt
     2641 atggcaaatg tagtaaatcg tttattacaa gaggatctat ttgaatcatt tgatatgcca
     2701 ctagatgtca caccacagca gattgcttca gaaatccttg attattgcga aaacagcgat
     2761 gtttcaaatg gattgatcat attagtagac atgggatctc tgaaagaaat ccatcagttt
     2821 tttaaaaaac agttatccgt accgcttttg attttgaata atgtgacgac acctttagca
     2881 atcacagtag gggaatgcct gcaaaaaaat gtatcattag aagagatagc agaagagact
     2941 gttcgacaga tccagccaga atggcgactg ctctatccag aggaaaacaa acccaaagca
     3001 ctcattacta cttgcttcac tggaatcggt acggcggtcc acttaagtga actgctagaa
     3061 aaaagcttac ctacggcttg tcagttgaaa atcatcccat atgaatatca gcaattaaaa
     3121 gataagaaaa acagtgaccc attattttcc atatacgaag tcgtaggaat gattggcacc
     3181 acagatcctg atatcaccaa tatcgactat ctttcattag aagacctcat ctcgggggaa
     3241 aagatgtctg tactagctga atggttggac actactatga atgcttcaga gaaggaaata
     3301 ttcaatcagc gcatcattcg taatttttca ttggaaaaag tactggattc cgtgactatt
     3361 ctagatacag agaaggtgat gggagaaatc gatcatttta tgagagaatt agaggtgcag
     3421 ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt atgtccatgt gagctgtctc
     3481 attgaacgat tgatccgaca aatgccaatc gaaacctatc aaggattaga gaaatggaag
     3541 cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt ttagtgtcat tgaagagcat
     3601 tatagtgtca tgatccccaa ttctgagatt gcctatatct atgatatttt gagtgaagat
     3661 acagagtttc tctcgataga agaagaattt tgaacataag caagttgttg ccactaattt
     3721 tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag aggagatgaa aaagtggata
     3781 gagaaattat cttagcttcc catggaaaat ttgcttcagg gatattagat tcattagagc
     3841 tgatctatgg gaaaaatcat tctattactg tattcgattg ctatactgat gagaattttg
     3901 atttagcgga aacagtaagg cagcttttta tgaggtacaa agataaagaa atcattgttc
     3961 tgacggatct ttttggcgga agtgtcaaca acgaattctt acaatatatc aatcgggatc
     4021 atgtctatct cgttgccgga ttgaatttgc cactattgat tgagctagta tctcggttgg
     4081 acgctgaagt agagacaact gttttgatcc agcaggtgct aactgattca aaaaaaatga
     4141 tccagttttg taacgagcgg ctcaatcaga aaatagaaga agacgaattt taggaggaag
     4201 aaaaatgatt gtattgacaa gagtcgacca tcggttatta cacggacaag tagctttttc
     4261 atggacacaa acaataggcg cagactgtat tttgattgct aatgatgatg tacctacgaa
     4321 tgagatacga aagacaacga tcaaattagc caagccacaa ggagtcaaac tagtgatcaa
     4381 atcaattgat gactcgattg ccgcgttgaa aagcggggtg acagataaat ataagttatt
     4441 catcgtagtg gaatcgatcg aggatgcata taagctggca gaagcgtatc cagaaatcaa
     4501 aaccatcaac ttaggaggga tcaaggcaaa acctggtact cgcagcatcg gaaaagcggt
     4561 aaatatttta ccagaagagg aggaacgctt gaaacgatta attgacaaac aaatagaagt
     4621 agaaatccgt caagtaccag cagataaaaa agtggatgta agagaagttt tgtaagaaag
     4681 gaagaacctc aatgattgta caagcagtct tattaggaat tgttgcattt gtcgcacaat
     4741 cagaatatgc actagggaca tcgttgcttt ctcgtccgat tgtgacgggg ctgtttacag
     4801 gaatcgtgtt aggtgatgta aaaactggga tcattatggg cgcaacttta gagttagcat
     4861 ttatcggatc gttctcagta ggtgcttcga ttccacctga tgttgtgacc ggtgggattt
     4921 tagggacggc ttttgctatt acagctggag ccggtacaga aaccgcgctt ttactaggtt
     4981 tgccgattgc tacgttgaca ttgatattga aaaatattta cttagggctt ttgattccaa
     5041 ttatgaacca taaagcagac aattatgcag aagaaggaaa ttataaagga gtcgagcata
     5101 tgcacttgct tgccggtatc ggtctttcct tgatgcttgg tgttgtggta atggtttctt
     5161 atatggtggg aagcaatacg atcggaaatc tattaaatat gattcctgac ttcgttcaaa
     5221 aggggttgtc agtagctaca gggttgattc cagctcttgg ttttgcgatg cttgctcgtt
     5281 tgctgatcaa caaacaagtt gcaccatatt ttttcttagg ttttgctata gcttcttact
     5341 tagagattcc tgtgaccgga gtagctattt ttggtgcgat tttagcagtt gtggtagtca
     5401 atattatgaa tgtccgtcaa ctaccacttc aaacaactgc aggggaggta gaagaagatg
     5461 aagaattctg agatcaaaaa cagcaaaata acgaaaaaag agctaaatca cgtattttgg
     5521 cgttcttttc aaatggagtt ttcttggaat tatgaacgtc agatgaatat ggcttatgtt
     5581 tatgcaatga ttccgatttt gaaaaagtta tatcagtcga aagaagaaat ggcagcagct
     5641 ttgaaacggc atctagagtt ttttaacacg accccacata tcgttacact gattttagga
     5701 atcacggctg caatggagga agaaaataag gatgatcctg aatttgatgt gactgcgatc
     5761 gatagtatca aaacatcttt gatgggacct cttgcagggt taggagattc tttcttttgg
     5821 ggaactttgc gattgattgc cacaggtgtg ggtacctctt tagccatgca aggaaatatt
     5881 ttaggcccta tcttgttcat tttgattttc aatattccgc acattctgtt tcgctatcta
     5941 gcaacaggtt ggggatatcg cttaggaacg ggttttttga aaaagatcca ggcaaacggt
     6001 atgatggaaa gtttgacatt aggcgcgtct attattggac tgatggtcgt tggagcaatg
     6061 actgccacga tgatcaccat caatattcct ttaaaaatcg gttcaggaga aaatgctgtg
     6121 actgttcaaa gtatttttga tgatatcgtt ccaaatatct tatgtcttgg agcatttggc
     6181 atagtgtttt atctattgaa gaaagaagtg aaaccattaa cgatcttgat cggactagct
     6241 ttctttggaa tttttggttc gtggataggg attttttaaa aaaggagcag agaaaatgtt
     6301 tacgatgcaa gattatatct atgaagaaaa ggaagtacta agtacgatct tgaaaaagaa
     6361 tgatttttcg acaagagaac atatgaaaaa aacagtgaat ttactgatct tagcaacggg
     6421 ttcatcttat aacgcttgtt tagcagcgaa gccagcattg gaatcttatg gtgatcttac
     6481 tgtcgatatt caagaacctt tttatttttt caattatgga aaactttctc cttcaatcga
     6541 tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct actgttgaag cagtaaaaat
     6601 gatacaaaaa caagggttac ctgttgttgc tatcacaaat gacgtgcaaa gtccacttgc
     6661 tttagaagct gcacaaatta ttgatttagg tgccggggta gaatcagtag gcttcgttac
     6721 aaaaggctat tctgcgactg ttttacagtt actactctta gggataggaa ttggaataag
     6781 taaaaataaa atcagcaaaa aaatagaaca agactacatg caacagttgc gcaaaataat
     6841 caaccattta ccagcgatta ttcaaaaaac ggaagaattt tttgatggat atcaaagttt
     6901 atttcgttta gcacagcgtt tcgtcaccat tggatatggt ccaaactggg gaacagctaa
     6961 agaagcagag acgaaattga ctgaaacaat ccgcgttccg tcacaaggat ttgagttaga
     7021 agcttatatg cacggcccat acctggaggc agatgcttcg catttgctat tctttattga
     7081 aggggaaagt gtaaataagg aacgttcgca aaagttgcaa agatatatga gtcggtacgt
     7141 tggagagact ctcaccatta ccacaaaaaa agcaagaaac gagaaaacac ttggattagc
     7201 aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc gttccgtttc aactattcgc
     7261 ttataaaacc gcagtagcta aaggtattga cttgaacaaa aagatttttg aagactttga
     7321 tacagtcttg aaaagtaaat tataagaggt ggaaatcatg ctgaaattca atgaagaaga
     7381 acaaatcaaa gcaataaaag gcgcactagc tttacgtccg caagtagaag aaatcatcga
     7441 taaactttat gcggaaaaat ttgacgctgt ctattatcta ggaatcggtg gaacgtatgc
     7501 ttcctcgatg caagctgtca cttatatgaa tgggaaaagc aacttgcctg tctttgtaca
     7561 gcatgctgca gaatattaca ctactggaaa caaaagatta acaaaagatt ctatcgttat
     7621 tttgtcctct gttaccggta caactcaaga agtagtgaaa gcagtagaac aaatcaagaa
     7681 agtaggagcg acactgattg gatttatcga taaagcgaac agtaagttgt ctcagctatg
     7741 cgattttgtg gtcacttatc cagctcctgg aacagaacaa atcaaatttt ttatggcagc
     7801 agatcgattg atgtatttga atggagaatt cgaagcttat tcagaatact atgaacagct
     7861 cgaacgttat ttaccaacag ggttagtgga agcagagaaa aaagcagatg catttggctt
     7921 atcatttgca gaaaaacatc gacgtgatag tatgcattat tttattggag cagggaatca
     7981 atggggagct gtttattcat atgccatgtg ttactgggaa gaacaaagct ggcttccatc
     8041 gaaatctatc catgctgccg agtttcttca cgggacgtta gagatcgtag aagaaacaac
     8101 cccagtcaca ttatttttag gcgaagatga acagagagtg ttatctgaac gtgtggctaa
     8161 acttttgcca aaaatttgca gcaattatac attgatagac acaaaagact atccagtaga
     8221 aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc ttattgatgc atgtagtgac
     8281 acaacgcatc gatgctcatg tggagcagtt gaactgccat ccattagaga tacgccgcta
     8341 ctatcgtcag tttgattatt aggatagact ggataaaaag aatataaaaa ataaagagag
     8401 cagcgataac tgctcccttt atttttttaa aatattggca tcaattacgt tataaaacac
     8461 attttttgca ttatctacag ttcaacttat aatctgttta atgggacatg tgggtgccaa
     8521 ccattttttg ttatgcaata gtactattta gttgttgcat ggatccgtag taaaagtcca
     8581 agtacaacga gttgcgccag tttacatagc gttttgtatc cagaatgttg actaaaacaa
     8641 aagttcctat gtcaaccgtc aacagagaca atctgtccgt tgcaggacct gacagattta
     8701 gaaatgttca atctgtctct tatgtatttt aaatcaatac tcactaggag aagtttccta
     8761 acctcctaat agtaatgctg gtagtaacat tgtcaaaact gcttcctctt aggaacaagc
     8821 aaagcataac gagtgtaaaa ataaaatgta gactattgta acatatcatt gattatttca
     8881 agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca atggaaatta gtcccaactt
     8941 taagaaatgt atactataat ggaaattatc aattatttat ttggaggaat atccatgaaa
     9001 caaaaaatcg cagttcttgg tcctggatct tggggcactg ctttagcaca aacattggca
     9061 gaaaacgggc atgatgtccg tatttgggga aatgtacctg aacagattga tgaaatcaat
     9121 aagtatcata caaatcagca ttttttacct gatttgacga tcccagaatc gattatcgga
     9181 tataaagaat tggcagaagc agtggataac gcagatgcta ttttatttgt tgttccaaca
     9241 aaagcgattc gttcagtcgc acaggaactt attggtaaaa tgaatactaa gccaatcatt
     9301 atacatgcaa gtaaaggact agaacaagat acgcataaac gaatctcaga agttttggct
     9361 gaagaaattc cagaagaaaa aagacaggca attgttgtat tatctggtcc tagccatgct
     9421 gaggaagtgg cagtacatga tatcacaaca attacagctg caagtattga tcaaaaagca
     9481 gcagcctatg tacaaaaact gttcatgaat gattatttca ggatttatac aaataatgat
     9541 gtgattggcg tagaaactgg tgcagcactg aaaaatatca ttgcaattgg tgccggcgct
     9601 atccatggat taggattcgg agatgatgca aaagcagcaa tcatgactcg aggattggca
     9661 gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc tgacatttat cggtttgagt
     9721 ggtgtggggg acttgatcgt tacttgtaca agtgttcatt ctcgcaattg gcgtgccggt
     9781 aacttgttag gacagggaca taagctggaa gaagttctag aaaacatggg aatggtcgtt
     9841 gagggtgtgg caacgacaaa agcagcagta gagctagctc aacagctagg tgttgagatg
     9901 ccaattactc aaacaatcta taatgttttg tataatggag aagatatcaa aaaagcagca
     9961 aaagaaatca tgttacgtga cggaaaaatg gagaatgaat tttctcttcg ttaaatagat
    10021 gatcaatagt taatc
//
LOCUS       DAFGZE010000096         9681 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.099, whole genome shotgun sequence.
ACCESSION   DAFGZE010000096 DAFGZE010000000
VERSION     DAFGZE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.099"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            51..260
                     /locus_tag="LTU54_002619"
     CDS             51..260
                     /locus_tag="LTU54_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297276.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="LTU54_002620"
                     /pseudo
     CDS             467..667
                     /locus_tag="LTU54_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="LTU54_002621"
     CDS             complement(1379..1984)
                     /locus_tag="LTU54_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297277.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="LTU54_002622"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="LTU54_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="LTU54_002623"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="LTU54_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="LTU54_002624"
     CDS             3019..3990
                     /locus_tag="LTU54_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8297278.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="LTU54_002625"
     CDS             4130..5443
                     /locus_tag="LTU54_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBL8297279.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="LTU54_002626"
     CDS             5486..6352
                     /locus_tag="LTU54_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBL8297280.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="LTU54_002627"
     CDS             6349..7197
                     /locus_tag="LTU54_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBL8297281.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="LTU54_002628"
     CDS             7211..8683
                     /locus_tag="LTU54_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HBL8297282.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="LTU54_002629"
     CDS             8700..9572
                     /locus_tag="LTU54_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBL8297283.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3037 a         1659 c         2019 g         2966 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa g
//
LOCUS       DAFGZE010000097         9297 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.100, whole genome shotgun sequence.
ACCESSION   DAFGZE010000097 DAFGZE010000000
VERSION     DAFGZE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.100"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            49..243
                     /locus_tag="LTU54_002630"
     CDS             49..243
                     /locus_tag="LTU54_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297284.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            512..811
                     /locus_tag="LTU54_002631"
     CDS             512..811
                     /locus_tag="LTU54_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297285.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(908..1342)
                     /locus_tag="LTU54_002632"
     CDS             complement(908..1342)
                     /locus_tag="LTU54_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8297286.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1574..2284)
                     /locus_tag="LTU54_002633"
     CDS             complement(1574..2284)
                     /locus_tag="LTU54_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBL8297287.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2274..3134)
                     /locus_tag="LTU54_002634"
     CDS             complement(2274..3134)
                     /locus_tag="LTU54_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBL8297288.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3139..3777)
                     /locus_tag="LTU54_002635"
     CDS             complement(3139..3777)
                     /locus_tag="LTU54_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBL8297289.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3792..4091)
                     /locus_tag="LTU54_002636"
     CDS             complement(3792..4091)
                     /locus_tag="LTU54_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297290.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4134..5618)
                     /locus_tag="LTU54_002637"
     CDS             complement(4134..5618)
                     /locus_tag="LTU54_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBL8297291.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5638..6099)
                     /locus_tag="LTU54_002638"
     CDS             complement(5638..6099)
                     /locus_tag="LTU54_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBL8297292.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6117..7184)
                     /gene="ulaG"
                     /locus_tag="LTU54_002639"
     CDS             complement(6117..7184)
                     /gene="ulaG"
                     /locus_tag="LTU54_002639"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBL8297293.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7402..8172
                     /locus_tag="LTU54_002640"
     CDS             7402..8172
                     /locus_tag="LTU54_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBL8297294.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8445..9272)
                     /locus_tag="LTU54_002641"
     CDS             complement(8445..9272)
                     /locus_tag="LTU54_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297295.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2878 a         1914 c         1472 g         3033 t
ORIGIN      
        1 acatatattt gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta
       61 gaagtaaaaa caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat
      121 cctttacttg gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata
      181 aagaacgagc tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag
      241 taaatccaat gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat
      301 tatagaatta gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata
      361 ccatatgatt atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat
      421 gaatcaaata tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt
      481 ataatgtatc agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga
      541 actacttaag caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa
      601 agtgacagag tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc
      661 aaaaggaata tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc
      721 tttaaaaaaa aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt
      781 tgataaagag gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata
      841 ctaagtctat cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta
      901 ttatatgtta tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag
      961 aaataccacc catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt
     1021 tctcagtatc atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca
     1081 aagcttcatt caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga
     1141 tatcttgaat aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct
     1201 tcccttcccc attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg
     1261 cagtgtcttt tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga
     1321 tcataaggga atcaatattc atttttttct tcctttctta tacaattctc cctctaataa
     1381 tacagttctt tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt
     1441 ccttaaaatt cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat
     1501 aaattttctt cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct
     1561 taccctagca tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt
     1621 tatctagtaa atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg
     1681 ccatcagaca aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc
     1741 cccatgtgaa cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt
     1801 ttctttcctt aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta
     1861 cttcttcttt cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag
     1921 tcgtgccata agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat
     1981 gagtatgagt aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga
     2041 gatctgaaga tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca
     2101 ccatttgatc tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc
     2161 ctagctcacg gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg
     2221 gcaatgccag atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag
     2281 acatcatagc cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct
     2341 cttcgaaagt cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct
     2401 agacgtttca aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca
     2461 tcacgaaact ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca
     2521 gtgatataat cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac
     2581 aaattgccta ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct
     2641 aaaaattttg aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg
     2701 ccatatttag aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa
     2761 ttcattgatt tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt
     2821 agattatgcg ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct
     2881 ggatcagcag aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt
     2941 acaccggagc tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat
     3001 cgtgctaacc gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct
     3061 tttacaaggg aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc
     3121 ccaatttgag ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc
     3181 ggccttcatc ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc
     3241 gatcccttca aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa
     3301 gcctagttca atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga
     3361 taacagcgca tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc
     3421 catggcttgt tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt
     3481 ttgtgctgcc tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc
     3541 tgcttttgca acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac
     3601 tagtttttta tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa
     3661 gcaaagaatt gtgcccactt caataatatc tactacatca ccaactttca taacatctcc
     3721 taatgcatgt tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt
     3781 agttccttcc tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt
     3841 tctttgactg acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga
     3901 gtaaacatat tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt
     3961 tttccctctg aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt
     4021 gctttttcca cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact
     4081 aaaattctca tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact
     4141 ctctgctttt ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt
     4201 ttccgatatt gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca
     4261 taacctagga ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc
     4321 ccgatataac caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct
     4381 acttgtatga ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta
     4441 ttggcataaa ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg
     4501 atcgttggtg atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca
     4561 aacaagaacc caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca
     4621 attcctggaa cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt
     4681 tcatcgacga atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa
     4741 gaagtcgtca taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc
     4801 aaataatctt tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa
     4861 gtagcaacca tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt
     4921 tttttgtctt gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct
     4981 aattttgcaa atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct
     5041 tctgtcaatt cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct
     5101 aaaatcagtc ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt
     5161 aaaatccaaa aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa
     5221 cgcagtttcg tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt
     5281 aataaaatca tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca
     5341 gcattttgtc caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt
     5401 ccaaccaaga ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag
     5461 taaccgaccg ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa
     5521 atatagccta ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt
     5581 gcaaaatacg tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta
     5641 attatattct gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc
     5701 aaccaatcca tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc
     5761 tggatctttt gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc
     5821 actttcaaaa cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc
     5881 atcacttttt tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc
     5941 gtatttttct acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc
     6001 gatcaataca agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg
     6061 gtaatgaatc aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat
     6121 aaaaacgacg gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa
     6181 aaattaaact caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag
     6241 atataaggtt taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc
     6301 tcttttggat cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt
     6361 gcattcaatg actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct
     6421 cgtgggtttt caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg
     6481 gcaaataaat tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg
     6541 aataaatagt ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct
     6601 tctggatctt cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg
     6661 atgtctttga ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt
     6721 ccccattttt gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa
     6781 ttttgatgaa ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg
     6841 attaaagcgt ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa
     6901 ttaggctgca tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg
     6961 ccattcccat gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct
     7021 tcatgacttt tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga
     7081 tccactactt ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct
     7141 actaaccact tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca
     7201 tatcgttgtt ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt
     7261 tatatgaccg tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata
     7321 ataaagacag atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat
     7381 gtccctcagg aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa
     7441 gaaattttgg aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc
     7501 cttgatgtat ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag
     7561 atcatacgca attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa
     7621 tctggtcctg aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat
     7681 tatgatacgc tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa
     7741 tctaaccatt tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat
     7801 aattgtagtt atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc
     7861 gacatcgcat taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc
     7921 ggagtcgatg tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa
     7981 atgatggtca acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga
     8041 ctgacatcaa agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa
     8101 ttttgttctg aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta
     8161 gattcatttt aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac
     8221 aaatttatcg ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt
     8281 atccatccaa tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct
     8341 aaataggaat caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt
     8401 cgaataaacg gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt
     8461 tcaatcacct ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata
     8521 tatctttcta aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc
     8581 aagtattcca atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat
     8641 aattggattg ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat
     8701 acaatgcaga ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc
     8761 acataaactt tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca
     8821 gctgcttgta agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa
     8881 aataatgctg catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa
     8941 atttgattta aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta
     9001 taagattctt ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg
     9061 attttgtctt cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa
     9121 agcgcactta agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt
     9181 gcttgttcac aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat
     9241 tcttttagta atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaat
//
LOCUS       DAFGZE010000098         9032 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.101, whole genome shotgun sequence.
ACCESSION   DAFGZE010000098 DAFGZE010000000
VERSION     DAFGZE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9032
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.101"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            69..887
                     /gene="purR"
                     /locus_tag="LTU54_002642"
     CDS             69..887
                     /gene="purR"
                     /locus_tag="LTU54_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBL8297296.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1390..2763
                     /gene="glmU"
                     /locus_tag="LTU54_002643"
     CDS             1390..2763
                     /gene="glmU"
                     /locus_tag="LTU54_002643"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBL8297297.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2857..3828
                     /locus_tag="LTU54_002644"
     CDS             2857..3828
                     /locus_tag="LTU54_002644"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBL8297298.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3834..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4002..4571
                     /gene="thiT"
                     /locus_tag="LTU54_002645"
     CDS             4002..4571
                     /gene="thiT"
                     /locus_tag="LTU54_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HBL8297299.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4652..5425)
                     /locus_tag="LTU54_002646"
     CDS             complement(4652..5425)
                     /locus_tag="LTU54_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297300.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5692..7392
                     /locus_tag="LTU54_002647"
     CDS             5692..7392
                     /locus_tag="LTU54_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBL8297301.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7362..7580
                     /locus_tag="LTU54_002648"
     CDS             7362..7580
                     /locus_tag="LTU54_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HBL8297302.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7600..8490
                     /locus_tag="LTU54_002649"
     CDS             7600..8490
                     /locus_tag="LTU54_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBL8297303.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8508..8792
                     /locus_tag="LTU54_002650"
     CDS             8508..8792
                     /locus_tag="LTU54_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297304.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3048 a         1550 c         1902 g         2532 t
ORIGIN      
        1 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
       61 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      121 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      181 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      241 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      301 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      361 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      421 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      481 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      541 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      601 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      661 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      721 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      781 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      841 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      901 agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
      961 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1021 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1081 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1141 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1201 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1261 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1321 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1381 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1441 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1501 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1561 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1621 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1681 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1741 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1801 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1861 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1921 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     1981 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2041 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2101 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2161 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2221 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2281 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2341 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2401 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2461 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2521 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2581 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2641 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2701 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2761 taagactttt gcaaaatcac ttgatttatt taaaaaatat gactactatt tattaggtag
     2821 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2881 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     2941 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3001 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3061 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3121 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3181 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3241 agaatgctga ctttggacct tcatgcagtt caaatccaag gtttctttga tattccagtc
     3301 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3361 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3421 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3481 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3541 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3601 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3661 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3721 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3781 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3841 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3901 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     3961 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4021 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4081 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4141 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4201 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4261 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4321 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4381 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4441 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4501 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4561 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4621 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4681 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4741 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4801 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4861 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4921 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     4981 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5041 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5101 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5161 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5221 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5281 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5341 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5401 tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt
     5461 attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa
     5521 tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt
     5581 tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac
     5641 tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata
     5701 aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa
     5761 ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa
     5821 aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa
     5881 agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa
     5941 aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt
     6001 ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa
     6061 ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga
     6121 ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac
     6181 tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga
     6241 gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta
     6301 ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg
     6361 acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa
     6421 gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct
     6481 actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa
     6541 ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc
     6601 ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt
     6661 aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa
     6721 attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc
     6781 tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg
     6841 aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca
     6901 agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct
     6961 tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta
     7021 ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc
     7081 aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt
     7141 atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat
     7201 cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg
     7261 ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca
     7321 ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat
     7381 atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac
     7441 tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag
     7501 accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa
     7561 aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt
     7621 ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac
     7681 cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat
     7741 tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta
     7801 gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt
     7861 atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt
     7921 agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga agaaaaaccg
     7981 gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat
     8041 tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat
     8101 actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag
     8161 gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag
     8221 caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg
     8281 gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta
     8341 ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc
     8401 aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa
     8461 atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg
     8521 acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa
     8581 aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta
     8641 ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca
     8701 cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg
     8761 tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata
     8821 aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt
     8881 gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt
     8941 ttctcggtat taagtaagcg ccccatagaa cagtacctta ctaacaaatg cacctcaaga
     9001 tatacttgag ctatcaagaa gtctggaggt tt
//
LOCUS       DAFGZE010000099         8950 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.102, whole genome shotgun sequence.
ACCESSION   DAFGZE010000099 DAFGZE010000000
VERSION     DAFGZE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.102"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(442..939)
                     /locus_tag="LTU54_002651"
     CDS             complement(442..939)
                     /locus_tag="LTU54_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBL8297305.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(979..1788)
                     /locus_tag="LTU54_002652"
     CDS             complement(979..1788)
                     /locus_tag="LTU54_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBL8297306.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            2122..2979
                     /locus_tag="LTU54_002653"
     CDS             2122..2979
                     /locus_tag="LTU54_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL8297307.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            2972..3874
                     /locus_tag="LTU54_002654"
     CDS             2972..3874
                     /locus_tag="LTU54_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HBL8297308.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            3907..4257
                     /locus_tag="LTU54_002655"
     CDS             3907..4257
                     /locus_tag="LTU54_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HBL8297309.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            4306..5229
                     /locus_tag="LTU54_002656"
     CDS             4306..5229
                     /locus_tag="LTU54_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBL8297310.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            5307..6200
                     /locus_tag="LTU54_002657"
     CDS             5307..6200
                     /locus_tag="LTU54_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8297311.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            6238..6801
                     /gene="amaP"
                     /locus_tag="LTU54_002658"
     CDS             6238..6801
                     /gene="amaP"
                     /locus_tag="LTU54_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL8297312.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            6815..7003
                     /locus_tag="LTU54_002659"
     CDS             6815..7003
                     /locus_tag="LTU54_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL8297313.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            7016..7918
                     /locus_tag="LTU54_002660"
     CDS             7016..7918
                     /locus_tag="LTU54_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL8297314.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            7950..8192
                     /locus_tag="LTU54_002661"
     CDS             7950..8192
                     /locus_tag="LTU54_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL8297315.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(8448..8819)
                     /locus_tag="LTU54_002662"
     CDS             complement(8448..8819)
                     /locus_tag="LTU54_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297316.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         3115 a         1549 c         1738 g         2548 t
ORIGIN      
        1 ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa
       61 gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc
      121 ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag
      181 gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt
      241 tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta
      301 agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga
      361 tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat
      421 cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag
      481 ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc
      541 tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc
      601 aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat
      661 ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc
      721 ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag
      781 ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg ctttagctaa
      841 tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg
      901 atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat
      961 atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc
     1021 attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc
     1081 aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat
     1141 gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt
     1201 gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc
     1261 tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa
     1321 tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt
     1381 ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt
     1441 cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata
     1501 gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat
     1561 ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat
     1621 tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac
     1681 gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc
     1741 atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta
     1801 ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat
     1861 gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg
     1921 ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt
     1981 agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga aaacaaagca
     2041 tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt tagaaaaaga
     2101 ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct
     2161 tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc
     2221 aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa
     2281 gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca
     2341 gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac
     2401 ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta
     2461 gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga
     2521 aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc
     2581 gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat
     2641 aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc
     2701 tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca
     2761 aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa tgaaaaatat
     2821 gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat
     2881 aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca
     2941 cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc
     3001 aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa
     3061 ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag
     3121 taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt
     3181 gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc
     3241 ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata
     3301 tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg
     3361 tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa
     3421 aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa
     3481 atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa
     3541 attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact
     3601 tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc
     3661 agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg
     3721 acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata
     3781 tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa
     3841 agttcaacca aaaacagatg tagttgttaa ataatagaag acaacaactg aagaggggga
     3901 gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg
     3961 gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa
     4021 ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa
     4081 tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat
     4141 tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa
     4201 aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag
     4261 tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag
     4321 aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc
     4381 gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc
     4441 gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca
     4501 ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg
     4561 ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa
     4621 tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc
     4681 acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag
     4741 cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa
     4801 caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa
     4861 acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta
     4921 ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat
     4981 ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc
     5041 atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt
     5101 tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta
     5161 gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat
     5221 acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca
     5281 atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact
     5341 tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata
     5401 tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc
     5461 gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg
     5521 ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg
     5581 aagtagcaaa atacgtgaaa gttgcaggac gtaagattgt cttactacct tttgatcttc
     5641 gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag
     5701 atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac
     5761 ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag
     5821 aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat
     5881 tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta
     5941 cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg
     6001 gtccaatctg gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat
     6061 ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg
     6121 ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg
     6181 gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg
     6241 aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg
     6301 tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc
     6361 ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct
     6421 attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt
     6481 ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt
     6541 cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg
     6601 aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt
     6661 aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa
     6721 gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat
     6781 gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt cgacatacaa
     6841 gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga tcaccgtggg
     6901 ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta tcggttttta
     6961 tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa catccatgaa
     7021 taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt ctgcacatcc
     7081 agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca aaacagcacc
     7141 agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag aagagaagaa
     7201 acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta aagttccaga
     7261 acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc cttctgacca
     7321 tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa aacttggttc
     7381 tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa tgcgcggtga
     7441 gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg aacaagtaga
     7501 cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac ttgtgaatac
     7561 agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag cagttgatat
     7621 ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa tgcaagaagt
     7681 aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca acgctaacgt
     7741 agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc aagacaaagt
     7801 aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata aagtaaaatc
     7861 agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg ttcagtaata
     7921 aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt atcgtcggag
     7981 gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg gggattattg
     8041 caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta ggttcttggg
     8101 gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc attttagtac
     8161 taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag gtcgtgaaaa
     8221 agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca tatttttcat
     8281 ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg gaataagagg
     8341 ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca attagatttt
     8401 taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta ataaaacaat
     8461 gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac gaccactttg
     8521 aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa gcaatggatc
     8581 agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt tactcttgtc
     8641 tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag taaacaaagt
     8701 ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag caggaaaaat
     8761 gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat aaatggcata
     8821 ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt tgtttgtgaa
     8881 gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa tcattattat
     8941 caaaataatg
//
LOCUS       DAFGZE010000100         8791 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.103, whole genome shotgun sequence.
ACCESSION   DAFGZE010000100 DAFGZE010000000
VERSION     DAFGZE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.103"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(177..971)
                     /locus_tag="LTU54_002663"
     CDS             complement(177..971)
                     /locus_tag="LTU54_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HBL8297317.1"
                     /translation="MSKQCAKCGGKIGLTSYKIKDKQIVCGNCMKKAGYGMTTPYKII
                     RSLNLEDLDKDPSEKRKLNISDHTAQNNHDIGNDRMRTTEEMVEFCLKYGYGKGMTKK
                     WTTHHFNLISDQLNSDEYVIFCFVGLHNYISSTKHDNNYAYALTNKRLIVAQQKMIGN
                     NVQSIILDNLNNISKKRGVLLGTLTIDTLGKVLNVAVDKDTVDRISDSLNEIIYNLKR
                     RNISATNSNTSTISSASEIRKYKELLDDGIITEEEFNKKKQELLDL"
     gene            1617..4424
                     /locus_tag="LTU54_002664"
     CDS             1617..4424
                     /locus_tag="LTU54_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8297318.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            4615..5106
                     /locus_tag="LTU54_002665"
     CDS             4615..5106
                     /locus_tag="LTU54_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297319.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            5209..6198
                     /gene="manX"
                     /locus_tag="LTU54_002666"
     CDS             5209..6198
                     /gene="manX"
                     /locus_tag="LTU54_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBL8297320.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            6231..7034
                     /locus_tag="LTU54_002667"
     CDS             6231..7034
                     /locus_tag="LTU54_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBL8297321.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            7055..7969
                     /locus_tag="LTU54_002668"
     CDS             7055..7969
                     /locus_tag="LTU54_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBL8297322.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            8052..8426
                     /locus_tag="LTU54_002669"
     CDS             8052..8426
                     /locus_tag="LTU54_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HBL8297323.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            8565..>8791
                     /locus_tag="LTU54_002670"
     CDS             8565..>8791
                     /locus_tag="LTU54_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL8297324.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSF"
BASE COUNT         2848 a         1448 c         1825 g         2670 t
ORIGIN      
        1 ccacacctta taaaaatctt tgacaaatct attcgaagga tcgaatttaa accataattg
       61 tgtttttcct tccattaaca tataaagttc acctcaaaaa gagtatacga acaaatgttt
      121 gtattgtaaa tagaaaaaga tgagctaaat taaatgccca tcttctttga tagatattat
      181 aaatctaata attcttgttt ctttttatta aattcttctt ctgtaatgat tccatcatcc
      241 aatagttctt tatattttct tatttctgaa gctgaggaaa ttgttgatgt attggaattt
      301 gtagcagaaa tatttcttct ttttaaatta taaataattt cgtttaaaga atcacttatc
      361 ctatcaacag tatctttatc aactgcaaca ttcaatactt taccaagagt atcaatagtt
      421 aatgtaccca acagcactcc tcttttttta ctaatattat ttaaattgtc taatataata
      481 ctttgtacat tattgcctat cattttttgt tgagctacaa ttaaacgttt attagttaat
      541 gcgtatgcat aattattgtc atgcttagtt gatgagatat aattatgcaa tcctacaaag
      601 cagaagatta cgtattcgtc actgttaagt tgatctgata ttaagttaaa atgatgagtt
      661 gtccattttt tagtcattcc tttaccgtag ccgtatttaa gacaaaattc taccatctct
      721 tcagtggttc tcattctatc attacctata tcatgattgt tttgtgcagt atgatcagat
      781 atgtttaatt ttcttttttc tgatggatcc ttatctaaat cttctaaatt caaagatctt
      841 attattttat aaggagtagt cataccgtaa ccagcttttt tcatacaatt gccgcaaaca
      901 atttgtttat ctttgatttt atatgaagtt aagccaattt tacctccgca ctttgcacat
      961 tgtttactca cattagttcc tcctagatat cagctttcta ctactattaa cattctatca
     1021 atagtgacca tactttgacc atacttttta gaaagaatag aaaattccaa gatatttaat
     1081 aatagaagaa aagcactaac atcatgggtt cagaagaatt gagagcttat caggaaacag
     1141 tataattgaa gtgtctgttt atccagtagc tcaagaagct taattgaatc ttatgattaa
     1201 agaggttgtg acaagacttt tgtcacagcc ttttttgttt ataaaaataa aagttgtagt
     1261 ggaggctacg aagtttactt tgtcttttta cacttaatat ttttttgcca atttggaatc
     1321 aggatgtttt gataactatc gttttcaatt gatggaagct atattgtttg ctatcagtga
     1381 aataattata tggtaaacag tgttcttttt cttgtttcca ttataaaaat cgtatcggga
     1441 tttttcgttc ttttaaattg aacatttcta tctatccgcg tgttatcaac aaaacaaaat
     1501 caggtaaata gttgacgaaa cagaaataaa tacatagtat tatgttttga agcctaagta
     1561 caatagtcta tagaaaagga gcgataccat tggcaaaacg cattgaccga atttatatgt
     1621 atgtcaaaga aaaaaccgct catttaacaa aggcagaata tgatcaagga gtaacaacac
     1681 aggaaattgc tgaagtgtta gggattcagc gaacaaatag cagtaaagat ctgaatcagc
     1741 tagtgcgcga aggaaaactg ataaagacag acggacgacc agtacgttac atatatcaaa
     1801 cactcgttac gcacgtaaag ccagttacaa aacatgtagt cagttacaaa gaaacgccgg
     1861 tctctgaaga aaaaaataca ctccaaatag atactaagga tatctttgca aaaatcattg
     1921 gagctaacgg cagcatgaaa aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc
     1981 caagaggatt aaattgtttg attacaggac ctaccggatc aggaaagact tattttgcac
     2041 acgcgatgta tcattttgca aaagcgcatc atgtaataga tgaggaaacg gaactgattg
     2101 ttttcaattg tgctgattat gccaataacc ctgagctttt gatgagtcat ttatttgggt
     2161 acgcaaaagg tgcttttaca ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg
     2221 atggcggtat gctgtttttg gatgagattc accggttgcc gcctgaaggt caagaaatga
     2281 tcttttactt tatggatcat ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg
     2341 aagcaaatgt acggatcgta ggtgcgacca cagaagaccc aggttcttct ctattagaaa
     2401 cttttgttcg gcgtatccca atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa
     2461 atgagaaagt cgatttagta aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa
     2521 agatttcttt gacagaagat gtagcaaaag cattgatcgg cagtgtgacc tatggaaata
     2581 taggacaatt gaagtcaaat atccagttag tatgtgcacg aggatttcta aatcatatgc
     2641 aatcacccga aatctcgatc acaatcgatg atttgacaga agggattcgt agcggattga
     2701 ttcaattagc aagcaaccgc agtgcaatgg cagagctttc taaaatattg gagccaaaga
     2761 taactgtagc accaaatgat acgatcatga aaatccaatc ggactcctat gagttacctt
     2821 ataatctata tgatatcatc ggagataaag cagctttact gaagtcggat ggtttggatc
     2881 aagaagcaat caatcatttc atttctacag atatcaacat ccatctgaag tctttctata
     2941 aagaccatgg tttttctttt aatgcagaca gcaaattagc agaatttgta gatccgaaaa
     3001 tcatagaagt gaccaatcag atttatgtaa tggtcaaaag atctttacca tatgagtttc
     3061 agcaaaactt tatttatgct atgagcttgc atatcagttc ttttttgaaa aaaatcaact
     3121 taggagaaga acggcatacg aatgataaca tccgtgagat ggcttttgat tttccagatg
     3181 aatatgctgt tgcgaaagaa gtgcgtagtt atatcgaaac gtattttcaa gtggagattc
     3241 cagaaagtga agactattac ttgaccgttc tactcgtctc gttacgggca aatcaagcaa
     3301 gcggcagaat cggtgtagtc gttgcagctc acggaaacag cacagcaagc agtatggtcc
     3361 aagttgtcca acagttgtta gatgcagaga acgtacgagc agtagacatg cctttggata
     3421 tggatccgaa aacagcattg gaccgaattg aacgcaatgt acaagaagta gatgaaggaa
     3481 gcggggtcat actcttagta gatatggggt cccttgcgtc gtttaattcg caaattcaaa
     3541 gagacactgg aattccagta cgtactgttg atatggtaac aacttctcta gttttggaaa
     3601 ctgtacgaaa ggtctcagta ttagggacag atctagatat gttgcatgaa tcattgaaaa
     3661 attttcgagg ttacgcagaa atatcttctg tagaatctgt tcaggaagca aaacctattc
     3721 ataagaaaaa agctgttttg gctatctgcg catctggcga aggtacagca caaagaatca
     3781 aagaattgat cgaacgagcg gtaaacaagc gtcaagaaac agaattagaa gttctagctt
     3841 tgtcgattgt ggaagtgaaa gaagaactgc cagttatcca aaaagaatat cagatcatag
     3901 ctactactgg gattactgat ttgaatatcc aagccccatt tattccttta gaacgtttta
     3961 ttgatcaaaa tatcgaagtc attttggatc agttgctgat ggaatcagaa ttggaggaaa
     4021 ctgaattcat ctccctagat gaagagtcag ctaaaaatac gtgtgttgag tttatctctg
     4081 ataacttcat ctttattaat ggtagtaagt taatcgatcc aatgtggcaa ttttctacac
     4141 aaatctctca gacgacggga atcggcgatg aagaatatgg attcaaaatc aacctagtga
     4201 tgcacacagc aggaatgatc gaacgtatta ttcggaatga accattgact gtggaagaaa
     4261 acgaattgac aaatacaacg aatgatcctc tttatagcca attggcagcc tcagttgttc
     4321 tgttggaaga tcagataaaa gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc
     4381 tagtccataa tcagttagat aaaaaagaat acactgtgcc atgaatacac aaaaacgata
     4441 cacttttata gtgtatcgtt tttgtgtatt ggttaagaat cctttaaatt caattagtgt
     4501 atcgtaaaaa agttggcacg ctttttgctt atagtagtgt gtgctaggag gtggttgttc
     4561 acacagaaat gataggggtt tctgtgaagt agtgaggaag aaggagtttt gcagatggat
     4621 attcgattgg cacgtataga tgatcgttta atccacgggc aagtcgcaac agtttggtcg
     4681 aagcaaacag gtgttgaacg tatcattgtg atcagcgatg tagtcgctca agataaattg
     4741 agaaagtttc tactaaaaga agctgcaccg ccggggatca cagcaaatgt aataaccgtg
     4801 cagaaaatga ttcaaatcta tcatagtgga ttattatcac gagcaaaagt gatgctttta
     4861 ttcaccaatc cccaagatgt tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg
     4921 aatatcggcg gtatgagttt tactgaaggc aaatcaatga ttaccaattt tgtttcagta
     4981 aatcaacaag atattgattc ctttcgttac ttgaacagtc aagaagtgga gttagagatt
     5041 cgaaaagtac ctgctgacag gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag
     5101 gtgtagcaag aatggttgtt ggaaaaggtc aaataattct gttgtaatgg ttagcaatta
     5161 ttaatatttt tgaatttgtt acaacaaaag taaataggag gtataaatat ggtagggatt
     5221 atcctagcaa gtcatggaca atttgctgaa ggaatcttac aatccggttc aatgatcttt
     5281 ggcgaacaag aaaacgtcaa agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg
     5341 agagccaaat tggaagaagc ggttgcttct tttgataatc aagacgaagt gttattctta
     5401 gttgatttat ggggcggaac accgttcaat caatcaaaca cattattcga agagcacaaa
     5461 gataaatggg caatcgtcag cggtttgaat ttaccaatgt tgattgaagc ctacgcatca
     5521 cgcttttcaa tggaatctgc tcatgaaatt gcagcacaca tcattgaaac agcaaaagac
     5581 ggtgtaaaag taaaaccgga agaattagaa ccggcagaag caccgaaagc agcagttgaa
     5641 gacgcacaac ctaaaggagc acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa
     5701 tatgtattag cacgtgttga ctcacgtctg ttacatggac aagttgcgac agcttggaca
     5761 aaagcagtgc aaccaaaccg tattatcgtt gtttcagatg cagtatcaaa ggatgatctg
     5821 cgtaagagac taatcgaaca agctgctcct ccaggtgtaa aagcaaatgt tattcctatt
     5881 agcaaaatga tcgaagtagc aaaagaccct cgttttggaa ataccaaagc attgctttta
     5941 tttgaaaatc ctgaagacgt attgacagct gttgaaggtg gcgtagacat caaagaatta
     6001 aatgttgggt caatggcgca ttcggtcggt aaagtcgttg taagtaaagt attgtctatg
     6061 ggacaagaag acgttgaagc gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta
     6121 cgtaaagtac ctaatgattc acgcgataac atggatgata ttttgaaaaa agcaaaagct
     6181 gaattagcaa aagcgtaata aataatatta aaatttatag gaggcttatc atgtctatta
     6241 tttcaataat tttagtcgta ttagttgcct ttctagctgg tatggaaggt attcttgatg
     6301 aatttcaatt tcatcaacca ctagtggcat gtacattgat cggtttagtt accggtaacc
     6361 tcgaagctgg tatcgtactt ggcggtacgc ttcagatgat cgctttaggt tgggcaaata
     6421 tcggtgccgc tgtagcacca gatgctgcat tagcatcagt agcatcagca attattttag
     6481 ttttaggtgg acaaggtgtt aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg
     6541 ctgttgcagg gttgttctta acaatgatcg tacgtacaat cgctgttcca atcgttcatt
     6601 tgatggatgc ggctgctgaa gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg
     6661 ctgtatgttt acaaggggtt cgtatcgcgg tcccagcagc agcgttgcta tttatcccag
     6721 cacaaactgt gcaatcattc cttgaatcaa tgccagcatg gttaacagat ggtatggcta
     6781 tcggtggggg aatggtcgta gctgtaggtt acgctttggt aatcaacatg atggctacaa
     6841 aagaagtatg gccattcttc gttattggtt tcgttgtagc tgcaatttct caattaacat
     6901 tgattgctct aggtgcttta ggtgttgcat tagctcttat ttacttgaac ctttctaaaa
     6961 tgggtggttc atcaaataat ggaggcggaa gcaactctgg tgacccttta ggcgacattt
     7021 tgaatgacta ttaatcttga aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc
     7081 aaaaaaagat cgtttagctg ttgcgtggcg ctctaccttc attcaaggtt catggaacta
     7141 cgaacgtatg caaaacggtg gctgggcatt ttctatgatt ccagcaatca aaaaattata
     7201 caaaacaaaa gaagaacgtt ctgcagcatt aaaacgtcac ttggaattct ttaatacaca
     7261 cccatacatt gcttctccaa ttttaggggt tacattggct ctggaagaag aacgtgcgaa
     7321 tggcgcgcct gttgatgatg tagcgattca aggggttaaa gtaggtatga tgggtccttt
     7381 agctggtgtt ggtgacccag tgttctggtt tacagtacgt ccaatgctag gtgcactagg
     7441 tgcttcatta gctatgggag gaaatatcct tggaccaatc atcttcttct taggttggaa
     7501 cattattcgt tgggcattca tgtggtatac acaagaattt ggctacaaag ctggttctaa
     7561 aattactgat gacctttctg gcggattgtt acaagacgta acaaaaggtg cttcaatttt
     7621 agggatgttc gtattagctg cccttgtaga aagatgggta tcaatcaaat tcttaccagt
     7681 tgtatcaact gtaaaattag ataaaggtgc ttacatcgaa tgggataaat tgccttcagg
     7741 tggtgaagga atcaagagtg catttgaaca agttaataat ggcttagccc tttctcctga
     7801 aaaggtaaca acattacaag acaacttaga ccaattgatt cctggtttag ctgcattagg
     7861 attaacattg ttatgtatgt ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct
     7921 tggtttattc gtagtcggtg ttgttggaca cgtcatcgga cttttataat ccataaaaat
     7981 atagctcggg cgttgtcccg agcttttttt atatagtata ctgttcataa acaaatatct
     8041 ggagggagaa aatggttcaa tcaatcaata caaaagtaga cttggtaatt gatgctactt
     8101 cgtttacggg tctgacagat tacgggcaaa tcatgatagg tgataatggt tttgagtttt
     8161 accatgcgcg agatagacgc aaatatatcc aaattccatg ggaagaagtg gattatgtac
     8221 tagcatcagt catgctaaag ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa
     8281 atggtacata cacattttct tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat
     8341 atgtagatga agatagaatg gtacaatcat tgagtttctt tgatgtagtg aaacgtggta
     8401 ttcgtagtat gtttaaacgc aagtaaaaaa taaagaaaac ttctaagagg aggttttctt
     8461 tattttttta ctttgcagaa gaaaatatga aatagcaaaa ccaattattt gtccccttta
     8521 aatcccatat gctaaaattg attagcaaag aaggaggtta tattatgagt tggttatgga
     8581 cattgattgt tggagccgtg atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt
     8641 cgatgggctg tgtaacgaat atcattgctg gactagtcgg ttcaagtatt ggacaagcga
     8701 ttttcggcga atggggacca aaagctgcag gtatggcact tattccatct attttaggcg
     8761 cagtgatttt ggtagcagtt gtctcatttt t
//
LOCUS       DAFGZE010000101         8553 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.104, whole genome shotgun sequence.
ACCESSION   DAFGZE010000101 DAFGZE010000000
VERSION     DAFGZE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.104"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(242..919)
                     /gene="nth"
                     /locus_tag="LTU54_002671"
     CDS             complement(242..919)
                     /gene="nth"
                     /locus_tag="LTU54_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBL8297325.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(926..1624)
                     /locus_tag="LTU54_002672"
     CDS             complement(926..1624)
                     /locus_tag="LTU54_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBL8297326.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1892..3076
                     /locus_tag="LTU54_002673"
     CDS             1892..3076
                     /locus_tag="LTU54_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HBL8297327.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3082..3525)
                     /locus_tag="LTU54_002674"
     CDS             complement(3082..3525)
                     /locus_tag="LTU54_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8297328.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3751..4038)
                     /locus_tag="LTU54_002675"
     CDS             complement(3751..4038)
                     /locus_tag="LTU54_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297329.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4209..5561)
                     /locus_tag="LTU54_002676"
     CDS             complement(4209..5561)
                     /locus_tag="LTU54_002676"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HBL8297330.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5834..6280)
                     /locus_tag="LTU54_002677"
     CDS             complement(5834..6280)
                     /locus_tag="LTU54_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBL8297331.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6516..7442)
                     /gene="cysK"
                     /locus_tag="LTU54_002678"
     CDS             complement(6516..7442)
                     /gene="cysK"
                     /locus_tag="LTU54_002678"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBL8297332.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7582..8469)
                     /locus_tag="LTU54_002679"
     CDS             complement(7582..8469)
                     /locus_tag="LTU54_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBL8297333.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2328 a         1754 c         1421 g         3050 t
ORIGIN      
        1 ataatccttt ggaatactgg acttatttta taggtatatt ggaaggcatt attttctaca
       61 tcgggtattt tcccaataaa aataacaact tttattttta tcgcaataaa ttgtaataag
      121 ttaccatcct agctactgag tatgtacatt tactttttca tgcaaaaaag ataaacgaaa
      181 cagcttttct ccataataac aagataattc atacatttat ttgcaattaa agaaaccacg
      241 tctacaggcg tggtttcttt tttagcgcct tttcttttag acgcatccga tttttcccat
      301 cttgacatat tgatagcaaa ggacatacct cgcacttggg atttcttgcc gtgcaatgat
      361 atctaccaaa aaaaatcagt gtatgatgtg tctttaccca taattcttgt ggtactttcc
      421 gcatcagtgt ttcttctact tccatgaccg tagcatctag tttgcagatc ctcaggcgtt
      481 ttgagacacg ttccacgtgg gtatctactg cgattgctgg tatcccgaat gcatccccca
      541 atacgacatt tgctgtcttt ctgcctacac caggaagaga catcaattct tctcttgacg
      601 ttggcacttg cccatcgaaa cgttcaatca gctgctgtgc acacgctttg atgttttttg
      661 ctttatttcg atacaaacca atcgttttta ttttaagaat gatttcatcg attgaagctt
      721 ctgctaacgc atcgggtgtc ggaaaagagg caaacaaatc tggcgttgct ttattcactg
      781 atacatctgt cgcctgggca cttaagatca ctgcaatcaa taactcaaat ggattattgt
      841 gcttgagttc tccatgtgct tcgggaaaca ttccatacat cgtttctaga gcttccattg
      901 ttttttgttt gcttaacata ttccgctact cctccaacca atttttcagt gagactttcg
      961 gcaattcttc ttgttgagct gactgttcta cttctctttg aagaagctgc tgttttcttc
     1021 gtttttggtc ttcttccact tgttgttttg tacgtagatt ttttctttcc cacgatagca
     1081 aaatgcggtc aacgtaatta aaactataag cctgatttaa tacggcttcc cgcagtgcta
     1141 gcttcaaaat ttccggctga taatggtctt cttctagcca ttgtccgatc cgctggaact
     1201 cgatcgaaga aagcgggcgt ccaaattctt gttcaaataa ttgatacact gactggattt
     1261 ctttttcttg tgttttttct tcgtattttt tttcttgtgt ttttaaatac tcacctaata
     1321 gatcatagat ggggtaaaga ttatatcgat ctgctttttt accgttgaca atcgtcgttt
     1381 ctatcttgat aaatccattt gttacgagac gatccagtat ttgaaaaatc tgatcttgtt
     1441 tgattcccat atccatagca atcatctgca aatcagggaa agtatctcct tcaagctgtg
     1501 ccatatgcaa ctgtagaaca aataaaaatt cttcagattg tagaccgatc ctatgataat
     1561 ttttaagtaa aagattagat atgactgttt gtccagcgct taagtaattt tctaaattca
     1621 acatgatttc tcctccgctc aatcacagta tacgtaaaaa aaacatggga agcaagaaca
     1681 ggtttctttt ctatttactt cactgatcgg tcagatttac aatgtcgtct acttgatttt
     1741 tgtttgttca tgtttttgat gcgtatcttc acttttcgga ataaataaat taagtagatt
     1801 tcgctgttcc ttagaatgtt gacaaggaaa caaaattact atacactatt tttgtttcca
     1861 agttaacatt attgcataaa ggagttttta catgtacagt ttcacaaata aacattctaa
     1921 agtttttgga tacgtctctg tcttattcac tggattagga tttactatcg tcagtcctgt
     1981 acttccgttt ttaacgctgc cttattctca ctccatacat cagcaagctt tctatatcac
     2041 tttgctcatg tctgtttatg ctctagctgc cttcttatct gcccccattc tcggtagctt
     2101 aagtgatcat tttgggcgtc atccgatcct tattatcagt ttattaggat caagtatcgg
     2161 atatttgctt tttggtttag gaaattcgat atggatgttg ttccttggtc gtatcattga
     2221 aggattgacg gcaggagaaa tctccacctt atatgcttac tttgccgaca tcacggaacc
     2281 taatgaaaga accaaagttt ttggatggat gggtgcttta gtcggtatag gaacaacgct
     2341 gggtcctatt atagggggtc tattagctga gttggggaat tctgttccta tatttattgg
     2401 agcactcttt actttcttaa atgccgttta tggctacaca tttatgctag aaagccttcc
     2461 aataaaaaaa cgttcagtag atttaagttt ctcccatgta agaccttttc accaattaca
     2521 acaattattc aagatttcat ccgttattcc tttattgaca gcaggttttg ctgtttggtt
     2581 ggcagctggc tctcttcaat ctatcttttc tcaattttcg attgacgcct ttcagtggaa
     2641 ggctgggttg gttggtttaa gcttctcttt gattggaatc ttggatactg tctcgcaatt
     2701 gttcattatg cctcgattgc tcaaaaaatt ttcagaacag cagatcacaa gaatagggat
     2761 gttcagtgaa atcctcgcct atctattcat cacattatct ggaattcttc tactgccgat
     2821 tttgttttta tttgggatta tctgttatgg tttcggtgat tctattttta ctcctgtatt
     2881 caatggccaa ctatccaatt ctgtctcaga taaccagcaa ggtcttgtaa tgggcggtac
     2941 ccaaagtata caatctctat cgcgcgtcat cggcccgcta atcgcaggac agctttatgc
     3001 tgcagcacca tttcttccga cggtattcgg atttttgctt atgatttttg cctattatca
     3061 ctacaataaa aaatagtagc ttcatttgct acttcgtata aattctctgt atcgaccgat
     3121 aaaacgaaat actgtgtcaa attcttcttt tgacatattt tcaaatacag tagcatctcg
     3181 ctgtacccat ttttgatgga gttgctcgtg tctattcctt attttttgtc cgattggcgt
     3241 caattgaaaa tagatttctt ttttattctc ttcgctttga taagtgttta tgatccctct
     3301 agctatcagt ttttttgtta gcttactgat ggctcctcta gtcatgtaac tagcattcgc
     3361 taattttgtc acgttaggat cagggatttt cccgatatgt tcaatcactt ctagttcatt
     3421 tagagtaaat cctgcaaatt ctttttccat ttctggttta ttacgatgaa taatctcatt
     3481 agctaaatcc agatattctt ctattagcag ttgtctttta tccatttcta caccttcttt
     3541 ttctttcatt atattgtttt tttgtttcct attcaacaaa gataaaaata ttttgtagga
     3601 aaacccaatc tgaaaccaag aaaaaaaggg gttgacaaag cttgtcataa cccctttttc
     3661 tgattaaaag atagtaaaga gacagtcatc ggtaattgtg atgatttttt gtcatcactt
     3721 aaggatgagc ggccttttca aaaactgtct ttaaagatca tcatcagttt ctagcacata
     3781 tggctgacct tcatatgtgt actctgtatt caatgtgccg tcattttcat ctgagtagat
     3841 gcgtctactg aaaggaaaac gatgagaaac attatctttg gcgtcatttg ctggcatcgt
     3901 gtaatattcc tcttgcgttt cattcaactg agagagtact tgatctgcca actcatcaat
     3961 attgatcgtt aatttcttcg aagcactttt ataatcttct aaaaatgcat cttgttggac
     4021 aaaatattct ttattcatct tctttctcac ctttcttttc aaaatcaaat aaagctgctt
     4081 gttcttttat gtaacagctt tatctttgct gcttattgtg acaaaaaatt cgaagaaaga
     4141 caactaattg tacttttcac aaaaaaagac agagggtcgt gacaaaattg tgtcacaacc
     4201 ctttttcttt attgtgcagt ttcgatttcg cctagcgctg cttgtgcaag taggttcaag
     4261 taattccatg ggcggtcaaa atgtggttgg aagaagaaat ctgaaagtgc gagatcttct
     4321 acagtcattt tattttgaac agctaaagac atggtatttg ctgattgtgt aatgtcgtat
     4381 ttggacatga actgagcacc tacgatacga ttcgttcctt tttcatagac tagctccatc
     4441 attacttttt cagtagttgg catgaactct ggacgataat tatcttcaaa tacagtcgct
     4501 tggacttcta gatcgttcaa agttgcgctt tctttggtca cacctgtcga accgatcgtc
     4561 cagccaaata ggtaaagtcc tgaagttcct tgagtaccac gatacgccat tttatgatct
     4621 gtcaagttat ttcccacaag caaaccttga cgaacggcat ttgttgccaa aggaatatag
     4681 ttttgcgtgc cacttggatt gtagttgact actgcagagt ctccagctgc aaaaatgtct
     4741 ggattacttg tctgcatgta ttcattcact ttgattgccc cgtttggcag tgtatctact
     4801 tttccttcga tcagttttgt atttggacgg aagcccacac ataaaatgac catatctgta
     4861 tcaaatgtat ctgttggtgt agctacttgt ttcacttttc cttcttcatc tgtgataaat
     4921 tcagtaacat tttcgcctag cgcaagagta acaccgcggt ctgtcaattc tttttctaag
     4981 atatctgtga atggtttatc tagatattta ttcaagatgc gatctaatcc atcaatcaat
     5041 gtcacttgtt ttccagattc gacaaaagct tctactaatt caataccgat atatccacca
     5101 ccaacaatta ctacacgttt tgcttctttc gcttgtgcaa taatttcatt tgcttgattg
     5161 tagtttttgc ataggagtac atttttcgca tcgattccac taatcggtgg gataataggc
     5221 catgaaccag ttgtcatcac taatttatca taagaaaccg tttctgtttc tcctgttttc
     5281 agatcttttg ccgttactgt tttattttct gtatcgacgt ctgttacatc gtgttccatt
     5341 ttaactgttg ctcctaatga agataattct tcaggatttg agtagaacaa gcctgctggg
     5401 tcctttacta ctccgccgac ataaagtgcg ataccgcaag ataagaaaga aatattgtca
     5461 tttctttcat atactgtcac ttctgcttct ggatgatttt ttaaaatact ttttacagct
     5521 gcagtacctg catgagtaca gccaattact actactttca tttatggtcc ctccgattaa
     5581 cattttgtcc ttcgacaaaa ctactataac agaaataaaa cggatacaac agtttaatgc
     5641 ttgtgaattc acaaaggttt gtttgtagtt tcattccttt tcaagcataa gaacccaata
     5701 tctacaagat tcttatttta ataatattca cttttatatc taagattgtg aataaaagac
     5761 aaaaaaataa aaaagctagc gtttttttta cacagctagc tttttttagg tataacactt
     5821 gtagaaactg tttttacatt tgcgcctttt ttgctgactc tttacaatcc ggacagagtc
     5881 catacaattc caaccgatgt tcattaatct caaaatcagt cagtctgctt gcagccattt
     5941 ctatatcttc aagccctggg taatgaaaat caacaatctt accgcagttt tcacaaatta
     6001 catgataatg acgctttgaa ctgaaatcaa atcggctaga agcatcgccg tacttcattt
     6061 cttgaacaaa tccaatcgaa gtaaataagc gaaggttatt gtaaacagtc gcaacactca
     6121 tatttggaaa ccgatgttca agtgctttat aaatctcatc agccgttgga tgggaatgat
     6181 gttcaatcaa atattctaaa accgcgtacc gttgaggtgt gattcgaatg ttctcttttt
     6241 ttaacttttc gataacctct tcgactaact tattttccat gtaaacaccc cacgttgtta
     6301 ttctcgtctt atcatactat ttttacattt taaaatccac ttaacatgaa aagaaaattc
     6361 aattttattg tattttaatg aaaaaagacg taaaacagtc gcgttcacta ttctacgtct
     6421 ctattcaaat aatttttctc atcataaatt tttgtgtgtt cttttcatct ttaaaagttt
     6481 tttacataaa cctatctgga gaaaaaccaa gtgccttata tctcttggta aagggctgta
     6541 gataaataac gttcaccatt gtcgggaacc acagctaata ccttcttgcc ttttcctaat
     6601 tcttttgcga cttttagcgc ggcagcaatt gcagcgccag atgagatgcc tactaaaatc
     6661 ccttcttttc gcccaacttc tctagctgtt tccatcgctt cgtctccact aatagaaagg
     6721 actttatcat acacagaagt atccagtgtt ttaggaacaa aacctgtacc gattccttgg
     6781 attttgtgag gtcccgcttc tttcccttct aaaatagcag attctgctgg ttctaccccg
     6841 attaattcga ttttcggata cacacgtttt aattctcggc cagcgccagt gatcgttcct
     6901 cctgtaccga tacctgcaac aaacgcgtcc aagccattta ctccaaatgc ttgatgtatc
     6961 tcaggaccag tcgtcttctc atgcacaaga ggatttgctt cattttcaaa ttgtaatggt
     7021 aaaaagtagc cgttttcttt tgccaaacgt tctgcttctc tgattgatcc agaaatacca
     7081 tccgcaccag gagtcaaaat caactctgcg ccgtaccctt tcatcaataa acgtctttcg
     7141 atactcatcg tctcaggcat cacgatgatt actttatatc cttttgcaac tcctaccatc
     7201 gacaatccga ttcctgtatt tccagaagta ggttcaatga tagtatcacc tggtttcagt
     7261 agaccatcgt gttctgcttt ttctatcata cttaaagcaa tacgatcttt tacacttcct
     7321 cctggattaa aaaactcaag ctttacaaaa acatctgctg aatcttctgg tacgatttta
     7381 tttaatttga cgattggcgt tttgccaatc aattcggtga ctgagttata gatagctgtc
     7441 atcttcaatt cctccttgtc tttgctcttc ttacttatca taatacaatc tgttcattta
     7501 tggcgagtga ttcgtctttg actagcaaaa aaaagacaaa gatataccat atatggtaat
     7561 ttctttgcct ttttatctaa atcaaacgtt tagttcttga ccaactaata aagaataact
     7621 ggacatagac atgccatttt tcgccattag atcatcaaca gacatgccaa atttttgtgc
     7681 aatatcccat agcgtatcac ctgacacgac tgtatatgtc tgaccattat ttgtatttac
     7741 atctgttgtt gaagatgatg acgttgaagt cgtcgtagta gtcgttgttg ttgtacttgt
     7801 ggttgttgta gccgaacctg ttgatggcgt atcaaaacgg gttaaattat aagtattaat
     7861 caatgagttc aatttggccc cataatttgg atcggtagca taacgacctg tcaagtacgc
     7921 tgttgcatca taaaaacttg ttgtattgct tttccacacg ccagcataat gataatcgcc
     7981 cgtggaaaaa ctggttgaac gaagaacatt cgcatggtct tggaaggatt cccaataaga
     8041 agaatatttt cggaatggct cattcatcgt tacccattgt ccgtttacat attctttggt
     8101 tgccatataa acgacctgtc cgttatagct tcctttcaca ccgaatagat tataatttgg
     8161 cgcactagct aatccggaag tgccatatgc actttccaac aacgcttgag cgatcatgac
     8221 tgaagcatac aagtcattac ttgcagcaac ttctgacgca gaccagccga tttgatcgat
     8281 aaacgcttga ggatcagcga ctgtttgaga tccttgttca tctgcatgaa catgttgcgg
     8341 taccgctgta accatcatcg gcccaactaa aagactcgtt ccaattaaag gagcaatcgc
     8401 ttttttattg atcaccattt ttcttttagg tctatggtgc cgggcactgc gggtcttcag
     8461 ctcttgcatt caaattcctc ctcaaaataa aaaaacttaa taaaaagttc tcttcttaaa
     8521 acaaacttaa ttaattaaat tatactttta att
//
LOCUS       DAFGZE010000102         8494 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.105, whole genome shotgun sequence.
ACCESSION   DAFGZE010000102 DAFGZE010000000
VERSION     DAFGZE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.105"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..173)
                     /locus_tag="LTU54_002680"
     CDS             complement(<1..173)
                     /locus_tag="LTU54_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8297334.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="LTU54_002681"
     CDS             complement(593..1714)
                     /locus_tag="LTU54_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBL8297335.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVMDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYKPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="LTU54_002682"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="LTU54_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBL8297336.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="LTU54_002683"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="LTU54_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HBL8297337.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="LTU54_002684"
     CDS             complement(4304..5215)
                     /locus_tag="LTU54_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBL8297338.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="LTU54_002685"
     CDS             complement(5232..6239)
                     /locus_tag="LTU54_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBL8297339.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="LTU54_002686"
     CDS             complement(6236..8365)
                     /locus_tag="LTU54_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBL8297340.1"
                     /translation="MKKKLFRIVGIVFISIVLLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="LTU54_002687"
     CDS             complement(8370..>8494)
                     /locus_tag="LTU54_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBL8297341.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2600 a         1726 c         1485 g         2683 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtttgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccatca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca ataggacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DAFGZE010000103         8103 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.106, whole genome shotgun sequence.
ACCESSION   DAFGZE010000103 DAFGZE010000000
VERSION     DAFGZE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.106"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            126..959
                     /gene="msrA"
                     /locus_tag="LTU54_002688"
     CDS             126..959
                     /gene="msrA"
                     /locus_tag="LTU54_002688"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBL8297342.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1072..2028
                     /locus_tag="LTU54_002689"
     CDS             1072..2028
                     /locus_tag="LTU54_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBL8297343.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            2317..3015
                     /locus_tag="LTU54_002690"
     CDS             2317..3015
                     /locus_tag="LTU54_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBL8297344.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            3165..4481
                     /locus_tag="LTU54_002691"
     CDS             3165..4481
                     /locus_tag="LTU54_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297345.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            4498..5913
                     /locus_tag="LTU54_002692"
     CDS             4498..5913
                     /locus_tag="LTU54_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8297346.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            6377..6697
                     /locus_tag="LTU54_002693"
     CDS             6377..6697
                     /locus_tag="LTU54_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297347.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            6723..7040
                     /locus_tag="LTU54_002694"
     CDS             6723..7040
                     /locus_tag="LTU54_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL8297348.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            7069..>8103
                     /locus_tag="LTU54_002695"
     CDS             7069..>8103
                     /locus_tag="LTU54_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297349.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLV"
BASE COUNT         2666 a         1319 c         1719 g         2399 t
ORIGIN      
        1 ataaggattc tctctttttt atacgaaaaa tctgtagggg gatgaagtaa tcgcttattt
       61 cctcacttta tgataaatta cagctaaagt cactagcttg tcttcttcgg aaaggaggag
      121 aatctatgga tgaggaacaa atattcattc aaacacttta taatctgatt ttgaatccga
      181 atacacgaga ttgggaacga aaagtattga ttcagacaaa gaatgacata agagaaaata
      241 tatccgttaa agaacagtta tcaaaactag aagcaacact acgtccatta gctatccgaa
      301 tgaatctgac gcctgatgta atggactttt atttattgat tacggaagga tttgataagg
      361 agcaaaaata tgacttttcc aagcatgcca tgcaagatgc tgactatcaa gaacgtgcag
      421 tatttgcagg gggatgtttc tggtgtatgg tcgagccgtt tgaatcgaaa aaagggatcc
      481 tatctgtttt gtcaggatac acgggcggac atgttgaaaa accaaattat gatcaagtaa
      541 gtggtggtta cacaggtcat gtggaagctg tggagatcat ttatgacaca agagaaataa
      601 gttatagcga gttgctgaca atctattggc aaattactga tcccacagat acatttggac
      661 aatttcaaga tagagggaag caatatcgtc cagtgatttt ttatcaagtc gaacgacaaa
      721 aagaattagc ggaacaatca aaacagaaac tagacagttc aggaactttc caccaaccaa
      781 tcgtcacaaa aatcgaacca gccggtacgt tttggccagc agaaaactat catcagcaat
      841 tttacaaaaa acaaccgaaa cgttataaaa aaatccagca agcacgtaat caatttctga
      901 tctatcaacg agtaaaaaac aaatggcaaa aaaatatacg aaaaaatcat tttgattaac
      961 tccttttctt taaaatgtag taaactaaaa atagaaaaaa gaaagcgttt taaaaagatt
     1021 tgatgaggag gagaaaaatt gtatattgca gatagtgcac gttatgaaaa aatggaatat
     1081 cgtaaatccg gtcattcagg tttgaaatta ccgattttat ctctcggatt atggcaaaat
     1141 tttggtgatt atgatcctat ccataatcaa agagagattt taagaggcgc attcgacatg
     1201 ggaattaccc atttcgacct tgcgaataat tatggaggcc ctgctggagc tgcagagaaa
     1261 aactttgggc gtatttttag agaagatttt caagcctacc gtgatgaact gattatttct
     1321 agcaaagcag gttatcatat gtgggaaggg ccatatgggg aatggggctc tcgtaaatcc
     1381 atcatttcaa gttgtgacca aagtctccaa agaatggggc tagattatgt tgacatcttc
     1441 tatcatcatc gcccagaccc tgatacacct ttggaagaaa cagcagaagc tttgatgcaa
     1501 cttgtaagac aaggaaaagc attatatatt ggtatatcca actataatgg agaagataca
     1561 aaaaaaatga ctgaaatttt aaaaagaaag gaagcaccgt tcattattca tcagatgcgc
     1621 tacaatatgt tttctcgtgc actactagaa gacgatctat ctccagtatt agaagaagaa
     1681 gggctagggg caatcacgtt cagtccgctt gctcaagggt tgctgacaaa tcgttatcta
     1741 catggtattc ctgaagattc tagagcacat cgtaaggaaa taccattcct ttcagaagaa
     1801 caagttggat caactctgga aaaaatcaaa gcactgcaaa caatcgccgt atcacgagga
     1861 caatcattag cgcaaatggc ccttgcatgg aatttgcgac aaaaatctgt tacaagtgta
     1921 ctggtcggag caagtcggtt aagtcagtta caagaaagtg ttcgaatgat ggataattta
     1981 gatttctcac cagaagaaga attgagaatc gaccaaattc tagaatagaa acgatgtaat
     2041 caaacttttt atccatatat taaaaaaaac aaaagatgac atcaagaatc gaatgaagat
     2101 cgtctgttat tgaacatcat attttttgtc gttcaaaata aaaatgatcg aaggctagga
     2161 gtgaatttta tcctagtctt ttttatggta aatatcttct tcgtttgtat gtctatgaac
     2221 ggattaatca attttgtata caaaaaatca tcagatgatt caaacgtgtt ataataatta
     2281 cgaaaaaata catcagatga aaaggaggta tgtggtttgg aagaaaataa aaccctagta
     2341 gaagtaacta ctgacaagat ccttagatat ttaaaggaaa tgcagttgca agtaggagat
     2401 aaactgccga atgaatatga attgtccgat tatttagagg tggggcgaag cacggtgcga
     2461 gaagctgtgc gggctctagc ttccaggaat atccttgaag ttcggcaagg agcggggaca
     2521 tttgtcagtg ataagcgagg gattgccaat gacccactcg gtttttcttt gattgaagat
     2581 caggatcgga tgatacgaga cttatttgaa ctgagatatt tattggagcc taagatggca
     2641 ggtttggcag cggaatatgc aacgaagaat caaatcgtcc aaatgaaaaa attaacgcaa
     2701 aagattgaaa cttcttttcg agaaggcaca caagaacatg ttcaattaga catcgatttt
     2761 catacgatga ttgcagaagc tagcggaaac gtagccttgc tgcatattat cccaatcatt
     2821 aatgaatcaa ttgctttgtt taataaaaat tatgatatcc agcatctgaa aatcgagacg
     2881 atcgatattc atacagagat cgttcaggcc atcgaaaggc ggaatgcact tgctgcgttt
     2941 gatgcaatga ctgtccatat ggcaataaat cggagagaat tacagaaaat aataggagaa
     3001 aaagaaacta attgacagtt catctgatgt attttatgat aaatacatca gatgaatttt
     3061 tgttttatat ttggtaaaaa agaggagtaa atagtttctg ttcatcgaaa tgtaagcgct
     3121 gactaataga caaaaagaga tgacgtcaag gagaagaagc aggaatgagg ggatctggaa
     3181 tgagttttat ggataaatta gccaatgttt tagggaacgt ggcaacgaga atcaataatt
     3241 taaggtatat catggtgata aaaaacgcat ttgctgcatt gattccggta attattacgg
     3301 gtgcttttgg cacattgttt tcagcaatgg tctttgactc ggaaaatggc ttagcacaga
     3361 taagtttctt gcgtttttta gaagaattaa agcctatcgc atctgccatc agctatgtca
     3421 cactaagttt cttgaccatc tatgctgttt ttctgatagg gatcgaatta gccaagctga
     3481 ataaagtgga tggtgtattc cctggtatca tcgcagtgat gtcttattta tctgttaatc
     3541 caactgttta tgaattagtg aatgaaaatc agactgttat agtagaaaac gtattggcta
     3601 aacaatatac agatacgaaa gggttatttt tgggaatgtt ggtctccatc ctttctattg
     3661 aactttattg ctggttgagg aaacagaaga aactacagct gagaatgcct gataccgtgc
     3721 caacgaatgt ttctgctagt ttttctgctt tattcccgac gattctgact gtatcgggta
     3781 tcgcggctgc gggatttatc atcaaagaac ttacaggaat gtacctttat gatatcattt
     3841 acaatgtcgt acaacgccca ttagaaggtg tcgtacaagg gttaccgggt attttgctat
     3901 tgatgtttat tgcgcaagtc ttttgggtga ttggtatcca tggaaatcaa atggtgaagc
     3961 cgatacggga accgttgctt ttagcggcaa ttgctgtaaa tacggaagct tttgaagctg
     4021 gaaaagaagt accgaacatt attactatgc cattttggga tatgtatatg agtatgggcg
     4081 ggtctggagt cacgatcggt ttactgattg cagtattgtt agttggaaaa cgggaagata
     4141 tgaaacaaat caccaagtta tcccttgctc ctgggatctt caacatcaat gaaccagtta
     4201 tttttggtat gccgatcatg ctgaatccaa ttttagctat tccgtttatc ttaacgcctc
     4261 ttatcactgg aacgattggt tattttgcaa ccgctttagg atttgcggcg aaagcagttg
     4321 taatggtccc ttggccaatg ccgccgatcg tcaatgctta tctggcaaca gcaggaaata
     4381 tcggtgcagt tattacgcaa cttgtttgta ttattgtttc tattttgatc tatctgccat
     4441 ttgtaaaagt agcaaatcgt gtggaaaatg atgggatcta aaaaacagga ggaaatcatg
     4501 aaaattacta ttccaaaaaa ttttattctt ggtgctgcat cttctgcttg gcaaacggaa
     4561 ggttggaaag gaaaaaaaga aggacaggat tcgtttatag atagctggta taaaaatgaa
     4621 cattttgttt ggcataatgg gtatggccca gccgtagcaa ctaattttat ggaacaatac
     4681 cagcaggatg tggatctgat gaaatccatc catctgacac attatcggac ttctatcaat
     4741 tgggctcgtt tttttactga ttatgagaac tgtatagtag atgaagaata tgctcagcat
     4801 ataagtgaaa tgatcgactc tttgatcgaa gcagacgtag aaccgatgat ttgtttggaa
     4861 cattatgaag tcccagcttt tcttttggaa aaatatgatg gctggtcttc aagaaaagta
     4921 gttgattttt atgctaagta tgctcaaatt gcctttgatc gttacggtga tagggtaaaa
     4981 aactggttta cattcaacga accaatcgtt ccccaaacgc gttgctattt ggatgctgtt
     5041 cgctggccgc atgaacaaaa cacaaaaaag tggatgatgt ggaattatca taaagtttta
     5101 gcaacggcta aagcagtaga gatttaccgc agcggaaatt atggtggaag gattggctgt
     5161 attttaaatc ctgaaatggt ctatgctcga tccaattctc cagaagacca aaaagctgct
     5221 tatatgtatg atttgtttta taatcgagta ttttttgatc cgatggtaaa aggatactac
     5281 acagaagagt tcctggagtt gtgtcaaaaa caatcgattt atttcgatcc tgcaacagag
     5341 gatttagaaa cgattcagca tcatacagta gatttccttg gaatcaatca atattatcct
     5401 aaacgcgttc gcgcccctcg ttatcaatgg aatgaagaga cacctttcca tcctgaacga
     5461 tattatgaag tctttgattt acctgggaag aaaatgaata attccagggg atgggaaatc
     5521 tatccgaaaa tcatgtatga tatagccatg tatctaaaag aaaattatca taatatccct
     5581 tggctgataa cagaaaacgg tatgggaaga gaatatgaag aacagtatat ggatgagaaa
     5641 ggaatcgtgc aagatgatta ccgtatcgaa tttatcggac agcatttgca ttacttgatc
     5701 aaagcaacag aagaaggcgc aaattgcgaa ggctatatgt tatgggcatt cactgactgt
     5761 gtgtcaccaa tgaatgcatt taagaatcga tatggattag ttcgaatcga attgaacgat
     5821 gaacgaagca gaacattgaa aaaatctgct tcgtggtatc agactttgat tgataagaga
     5881 gaattggatt tgcctgacga aatctataaa taaatagaag ttttttgtag atgttcattg
     5941 tatttactgg ttatttgaaa taaaagaggt tgtgaatcca gcgttttgag ctaataccca
     6001 gggtcagctg attgaacacc gtttattgag aggttgtgaa aaagctgtcc tgcatcaagt
     6061 aataagaata gcaaagtaaa aacaacatct tatgttttta acttggttgg acttattacc
     6121 gcagatgcaa gcttttgaat accgtttatt tgaaaaaagt tgtgacaagt tttgtgtcac
     6181 aacttttttt gtgcacgtta aaagaaaaaa ggtaataaaa attaatttgt gtgtttagtg
     6241 tgcgctttct tttttagtgt gtatgcaact tggtatagac cttaaatgtg atgatatcaa
     6301 ctttttaaag cgcttttaaa gttggcacgc tacttgctta tatacaagtg taagtataga
     6361 aaggaacgtg tttaaaatgg aaaagaaaac aatcatgctt gtatgttcag ctggaatgag
     6421 tacaagtctc ctagtcacaa aaatgcaaaa ggcagcagaa gcaaaaggaa tggaagcaga
     6481 catctttgct gtatctgctt cagatgcgga taatcaacta gaatctaaaa atgtagacgt
     6541 tttactttta ggaccgcaag tacgctttat gcaaaatcaa tttgctgaaa agctagcacc
     6601 taaaggcatt cctttagatg taatcaatat ggctgactat ggcatgatga atggtgaaaa
     6661 agtactcgac caagcagaaa aactaataaa caaataataa aaattatcgg aggatacaat
     6721 aaatggaaga tcaaaaaaat ctagaagcca tcatgggtct gatcatgttt ggcggaaatg
     6781 caaaaagcga tgcaatggaa gcgattgctg ctgcgaaaaa aggtgatttc actttagcag
     6841 atgaaaaaat caaagatgca gaggaatctt tagtccaagc acatcactct caaacagaga
     6901 tgctgacaca agaagcacaa ggaaatcacg ttgaagtcac gcttttgacg gtccacagtc
     6961 aagatcatct gatgacatct atagctttta cagatttagc aaaagagatc attgatctgt
     7021 atcgtcgatt gaacgcgtaa tctacagtaa agaaaagagg gaagatttat ggataaattt
     7081 gttgcattta tggagaaaca ttttatacct gtagcatcaa aaattggtgc acaacggcat
     7141 ttagttgcga ttcgggactc atttatggtg agtatgccgt taatgatctt aggagcatta
     7201 gcagtaatga tcaataactt accgattccc gggttccaag aattgatgaa ctcaattttt
     7261 ggtggtgaat catggaaagg attcggggct gctgcttgga acggtacatt tgccattctt
     7321 tctgtcttga ttgctttctt gcttgcttat catttagcta acggctaccg aaaagacggt
     7381 gtttccgcag gtgtgatttc tttaggctct ttcttcgctt taggcggagc attaggtatg
     7441 agctcaaacg gtttgtttat tgctattatt gttgggatta tttcaacaga aatctttgta
     7501 cgtttaagcg gaaatgaaaa attgatcatc aaaatgcctg atggtgtgcc accggcagtt
     7561 gcaaaagcat ttgcttcatt actaccagca atgatcacta tctctgcgtt tgctttagtt
     7621 gctgcaattt ttgctgcatt cggtgttgat gatatcgtag gttctttcta tacagtggtc
     7681 caagaaccat ttatgggact tgcaaactct tatccatctg ccttactgct tgcatttatc
     7741 acaccattct tatggttctt tggtttacac ggagcgaata tggtcgatcc tttgatgcaa
     7801 acaatcaatg cgccagccat tgaagcaaac gttaatgcaa tcgcagctgg acatagcgca
     7861 ccgtttatcg tgaacaaacc tttctttgac agctttgtca acttaggagg aacaggtgca
     7921 actttaggtt tactgttagc tatttattta gtaggacgta aaaacaaacc atatatggtt
     7981 gtaacaaact tgtcgattgc gccaggtgtc ttcaatatta atgaaccaac gatgtttggg
     8041 ttacctatcg tgttgaaccc gatcatgttc attccattta ttttgacacc aatggtctta
     8101 gtt
//
LOCUS       DAFGZE010000104         8050 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.108, whole genome shotgun sequence.
ACCESSION   DAFGZE010000104 DAFGZE010000000
VERSION     DAFGZE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.108"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..366)
                     /locus_tag="LTU54_002696"
     CDS             complement(<1..366)
                     /locus_tag="LTU54_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBL8297350.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSY"
     gene            complement(363..605)
                     /locus_tag="LTU54_002697"
     CDS             complement(363..605)
                     /locus_tag="LTU54_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBL8297351.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            complement(1151..1681)
                     /locus_tag="LTU54_002698"
     CDS             complement(1151..1681)
                     /locus_tag="LTU54_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBL8297352.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1863..2519)
                     /locus_tag="LTU54_002699"
     CDS             complement(1863..2519)
                     /locus_tag="LTU54_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL8297353.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(2646..3119)
                     /gene="dfrF"
                     /locus_tag="LTU54_002700"
     CDS             complement(2646..3119)
                     /gene="dfrF"
                     /locus_tag="LTU54_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL8297354.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(3538..3756)
                     /locus_tag="LTU54_002701"
                     /pseudo
     CDS             complement(3538..3756)
                     /locus_tag="LTU54_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3787..5001)
                     /locus_tag="LTU54_002702"
     CDS             complement(3787..5001)
                     /locus_tag="LTU54_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL8297355.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(5342..5584)
                     /locus_tag="LTU54_002703"
     CDS             complement(5342..5584)
                     /locus_tag="LTU54_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBL8297356.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(5616..6173)
                     /locus_tag="LTU54_002704"
     CDS             complement(5616..6173)
                     /locus_tag="LTU54_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBL8297357.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(6186..6374)
                     /locus_tag="LTU54_002705"
     CDS             complement(6186..6374)
                     /locus_tag="LTU54_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HBL8297358.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(6387..6953)
                     /gene="amaP"
                     /locus_tag="LTU54_002706"
     CDS             complement(6387..6953)
                     /gene="amaP"
                     /locus_tag="LTU54_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HBL8297359.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            7370..7543
                     /locus_tag="LTU54_002707"
     CDS             7370..7543
                     /locus_tag="LTU54_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297360.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
BASE COUNT         2445 a         1633 c         1150 g         2822 t
ORIGIN      
        1 atacgatgta ctaggaataa tttcttcata aatcttgcca taagtttcat atattttatc
       61 catagcacca atatgctcca caaccaaata tttataagac ggtatttctt tatacagaaa
      121 gccctctggg gtaatggttc tttggggaat agaacagaaa taataaagtt ttccatttct
      181 tctttccata aaagcatatt ttacccaatt tcctgattgt gaaagatacg cttttttcaa
      241 actactgtta aactttctcc aaaactgtgt actaatctga atattctttt tttgataatt
      301 agtcagttct acttcctgcc caataactgc aaatgcgttt aattccttta tagaaaaatt
      361 tatcatttta ccttgtgagc agtgataacg gtataactgt acgcatttac cgttataata
      421 caattatcaa tcgttatata ccagtttttt ccttttcttg tgatttccgc attagaaagg
      481 ctaatttttt ctttgcacca ttcaattaca ttttctacat tcaaagataa gttcttttta
      541 attctaataa cgccaagttc agttgtgtga agtcttccta agttttccag taattcattt
      601 tccatatttt gatacctcat ttttcagcaa atttatttgt atgttttata ccccaattat
      661 aacattctca tatcagcact acaataaaaa taattaagca ccaataatct tattgaacat
      721 ctccaaccgt aaatctttca caccatcagt gataaacact gagctgacag caattacgac
      781 aactaccata attgcctgca cttaaatttc tgcaaaagaa aacgacaatc aatttcttat
      841 tttttgaaat caactgtctt tctcatattt actattcaat tttcatagtg atataggtac
      901 aatttatatc actactgtaa aaatggggtt ttgccttata aatacagcgt tttccgcttg
      961 ttagtgtata gtgtttcttg ttttttgtgc cccccttgtt agataacggg ggctttgaac
     1021 ggctcgcaag ctcgccgaac cgccgaagcg aaccgccccc ttgcaagcat ggcggaaaaa
     1081 tcttaaggat tttcccgcca acgtggtcgg atcgctccgt cggtttaaca gcttttctgt
     1141 ttctctctag ttatctacgc atataaatca aatcggttat tccgttataa gtctgtgttt
     1201 tacaaagcct taactttatt tccctgtcta tgttgcccaa aagacgaacc ccatttccta
     1261 acagagtagg aataacagaa atgtaatatt tatcaatcat atttttgctt accaactgct
     1321 gaacaagatt tgctccgcca caaatccaaa tatcctttcc tgcttcttgt tttagccgtt
     1381 ccaataataa aacaggattt tcacttgtaa aatgaatttg ttgggaagaa ttgcactctt
     1441 tatgtgttaa tacataagtt gtaaatttat tatataccca ttcttgcgga gaaagttcag
     1501 taacaacctg atgagaagta ttccacccca ttaagacagt atctatatct ttggtaaatt
     1561 ctgtataagt gtcgatgttt tcattgtcat ttccatgacc atttagccaa gctactccac
     1621 cattaccgtc cgcaatatat ccgtcaaggc tcatggcaat aaataaaact acttttctca
     1681 ttttttctct tttataaata caaacaggaa tttatttctc ttttttactc ataagggaac
     1741 aactgattgc tacaaataaa cccattaaaa tccatggaaa tgctgctctt ataaaatcac
     1801 taaccatact ttattcctcc ttaaattcca atttccttag ttctacaaac tgaaattttt
     1861 gcctatgaat gtatcttgtt ctttatacgc aaacaggctt ctgtgtgcat aatccaatgt
     1921 cttgaaaacg gcatttgaat tagtccacaa atatctttat tacaccaata atcaatcagc
     1981 caaattgctt tttcattgtc gtttactaca ttcaacgatt ctaagtatcc ttttctttct
     2041 ttcggtattt ttcttttcaa ttcatcataa gataaccgtt caagcatttt ctcagtgctt
     2101 tcccatactt cgaaaatata taaatataat tcttccagat taagctgttt tgaaaagtct
     2161 gcaatctgct gtttcatgag ttcatttcct gttgtaatga taggtgaatt gatacgctct
     2221 tgataattgc ctgcaaaaaa tacttgttca tcttcattta tcactgtatg cacaactata
     2281 tcttcaatgc ggaaaatatg ccaaatagaa taagcaatcg tcttactatg ataaccatca
     2341 gcgtttatga aggggattga gttaaaatct tctctgcata attcttcttt gaaagatact
     2401 aaggtttgta ttaactgact tcgtaaagta agtaaagtat caatacccct cttataagta
     2461 tctttctttt ttatttgtgc ctgcattgtt ttattaagtt cagaccattc cttattcata
     2521 ttcaatcctc tctcaaattc tgattttatc gccccataca aactggaatt tgtcttacaa
     2581 caatttatat atgcataaaa agcaatatat ttatgaaaac accctaaatc caaaatttac
     2641 tcaattcatt ttttccttac ataaaatgtt ctcgtatatt tcacttcctc accaagtgtt
     2701 tcccctatca aaacttcaaa atcattgata tcaaattctg gaaagaatgt atctccatct
     2761 tcaatattta aatctacttc tgtgatatac attttatcta ctatttgcaa agcttcctta
     2821 aatagtccat atccaccaga tatgtataca tctcgtcctt tagccaataa taatgcatct
     2881 tctaatgatt taactgaaac taaattatct ccttgatact ctgttgtggt ggaaacaaca
     2941 atattcattc tattaggcaa cggatgaccg atttcttcat aagactttcg ccccataata
     3001 accacattac ccgttgttaa ctctctaaat tgcttttgtt ctccctttat tttccatggt
     3061 atattaccat tcttgcctat aacattattc tttgacctcg caacaatcaa acctatcatc
     3121 tatttcatcc tccataatat gatgtcgtac ctgtgattcc atcaagtgca ccacatctta
     3181 acaaactcaa aattatctcg aactaaccag ctcgacaaat tccttttttg tcttcatctg
     3241 cattatatca tattgttata agcattgcca tttcttttct gcttttgaaa agttacttat
     3301 cagcctatgt tttgtaaagg gtgatattca gcatttctca cgcaacaagc ccttgacaaa
     3361 ccatagacta ataagtttta aggtaatcaa gcagaaatga aatagtctgc tgtttcacgg
     3421 caacaaaaaa gagataccaa cacacttatg tagtatctct tagtctagta gcaatattct
     3481 tttgtttgca agcaggtaaa catgggctgt ttcttaatat ctttttgttt gggaaacacc
     3541 tttttcccat cctttcaccg ttgccatctc tgctgcgcac aatgccacag ggataaacca
     3601 tagcaaaccg cctaataaca agaaaaatac taagctgaag cctgaggtcg cgaatgtggg
     3661 atattcatat aatgaaacga ccattgagat ggtcatagcc aaaaagccaa agaacgttaa
     3721 tttttttgca ggaattttcg ttttgctttg ttgcatgaat cttcctcttt tctatttgaa
     3781 tttgtttcaa ttgagtttgt tgccaaagaa gaaaaagtcc aatccttctt cttgttcttc
     3841 ttttgtagta gtgatcgttg taaagtgcat cgccgatttt aaaatcactg ttgtcagcac
     3901 ataggcatac acggcagtaa tgatgacacc aatcacttga atccctaaga agtaccagct
     3961 gcttggattc gttagtaaat ggctgttaga tgcaaagatc cctatcagaa tcgtacctgt
     4021 gaatccgccc atgccatgaa caccccacac atcaagtgca tcatcccatt gtttcttttt
     4081 acgaaaatca acagcagcat gacagacgat acccgcaatg atcccaatca gcatggcact
     4141 ttttgcttct acataaccgg cacaaggcgt aatggctgcc aatccagcaa cggatcctgt
     4201 tagtacgtcg acaaaatcaa agccatttcc ttttactttt gcaatgatca gccaagtaat
     4261 catcgcaaat gcaagagcga tgaatgttga accaaatgca ttgactgctt gttcatctgc
     4321 tcgaagtgca ccaccggaat taaaaccgaa ccagccaaac caaagcagtc ctgtaccgat
     4381 agcagctgcc ataagactgc tatgctgact tctttctcct aaaggaatct ttctttgacc
     4441 aagtgtgagg atacatgcta aagagccgaa tccagctgtc gtgtggatga ctgctccacc
     4501 agcgaaatct ctaaatccca tctgatccaa aaatccgcca ccccatatcc agtggcaaac
     4561 agggaaataa atcagcagat tccaaaaaat aacaagtata atatagcctt ttaaattcaa
     4621 tctccctgca aaagctcctg tcatcaaagg aacggtaatc acgataaaca tcagttggta
     4681 aagaaagaac attaaaaatg gaatcgttgc accatgaaac atgttgggga agcttgtaac
     4741 atgtctcatc aaaaagaaat ctgccgggtt tccaataaca ccgccgatat cccgtccaaa
     4801 ggccaatccg aatccaccaa atacccacat aatcgttgtt atcccaacgg agagaaaaga
     4861 ttgagacatg atcgttagta tattattctt ccgtgctaat ccgccgtaaa aaaaagctag
     4921 accaggagtc atcaggcaaa ccattgctgt acataaaatc atgaatgctg tatctcctgt
     4981 attgataggt aatgtattca tttttttcgc ctcctttcta cgattacgag tataaaagaa
     5041 ggtgtagttg tttttttctt ctgttttttt gaattaatac ttgcatttat aaaagacaga
     5101 caattaataa aagcttgatg tgttgtgctc tttttcgttt ttcacacaaa taaagacaaa
     5161 aaataaacgg tcttcttttt atagaaaacc gtttattttc gtcttttttt atttttaaag
     5221 atattttttc ataaggaata aagaatactt catataaaat atataaaaaa gaaccgcagc
     5281 gagtgtcgct acggttctca aatttgttct tcatttcaac ataagttttt cttcaatttg
     5341 cttataattt acgtgcaaag aatgaaacga ctaatactaa aatgattgca ccgatcaatg
     5401 atggaatgat agccattcct gcaattgttg gtccccaagt tcctagtaaa gcttgtccaa
     5461 ttgctgaacc tactaaacca gcaatgatgt ttgcgatgat ccccattgat gatcctttat
     5521 tcgtgattgc tcctgcaatt gctccaatga taccgccgac gattaatgcc caaataaatc
     5581 ccattttaaa ttcctcctaa ctgacttctt acttcttatt gtacacgtgg ttcgttattt
     5641 tctttgactt tttgtgcacc tttgcctaca gcagattttg ctttatctgt ttggcgtgaa
     5701 gcaaattgtc ctgtggattt tgctgcttct gttactttgt cttgtactgt ttcttgatct
     5761 ttttggtatt cttcttgtgt tttgatgtct gcaacattca cattcacttc gataacatct
     5821 aaatgtgtca tattgtttac ttcagtactg atcaattctt tgatttggtt gtaaatatct
     5881 tcgatgtctt taccgtattc tacgatcaca tctagatcca cagctacttg tttcttacct
     5941 acttctgtat tgatacctgc tgttacgtta tctgtgttga ctaatttttc agccatattt
     6001 gagaaaaatc cgccatctaa tgtcaataga ccatctacat ctttcaatgc ataaccgatg
     6061 attttttgga tgactttgtc ttcataagtc aattcaccat tcatcatgcg gttttctcct
     6121 ttttcatttg ccaaaactga tccatttgtt gtattcatgt ttttgttttc catctttgtt
     6181 tcctcctatg atttaaaaaa ttgatcaact attcctgact gtttcaaata aaaaccgatt
     6241 cctgcgccaa ttgctgtcat gattatgatc aacaatgtct tgaaaaatcc aatcgttaac
     6301 cataaaacag ctagcagtag tccaagaccc ccaaagataa tagggagctt gtatgctgaa
     6361 aatagttctt tcattatttg gccctcctat tccactcgag agtgcttgta gtctcttgta
     6421 tcttcttgtt cttcataacc gctgtatgtg actgccactt tgaccgtttc ttttgttccg
     6481 agtactttag ttacttcttc ttcgatatct tgcatcaata cacctgtttt ccctactaat
     6541 gaagaagttc gtttcaagtc tccgttgaca tggatatgga ttcgattttt cgttgaacgt
     6601 atccgtactt taggtgaatg aatgaactct tcttcgtgta aatgggaacg taccaaccct
     6661 tcgatcgctt ttttgtcgat tgttagtttg ccatcttccc gtttcataac gaaagttcct
     6721 cttgactttg gatagaaaag aacgactagc aagatgataa tagcaagaac ggcaaatact
     6781 acagctgccc agaaaatata ttgttgcatg tagtaattcg ttaaagtaaa gaagcgaaat
     6841 cgttccaatt gttcaggcat aatatacggc gcattgatca acgcaatcgc tagaaatact
     6901 gaaagtagca gtaaactcat gataacaccg attattttta accccttatt cattttccac
     6961 ctcctataaa cttattttta ccctgtaaat aactctaccg acaatcacag tatagatagc
     7021 gaacagttag catagcatca gtaaataaag cgctaccaaa cattgctgga tcataacggc
     7081 gttttattgc cagtctgata ctgaaataac aattgcttac ttattctcta tttacttttg
     7141 attgctggct atacttaaag gatattattc aagattaaaa attgcaaaga ttatgctcag
     7201 ttattattac aagattctat ttatttatta aattttttcg gagaattaaa aaatacaata
     7261 aataacttta gttactttaa tgcgttctta ctttattttt atattatcat ttttcttatt
     7321 cacttctttg gtttatacta ttcttataaa taaaaacgag gtatgtccta tgatgaaaag
     7381 attgtactat tcattgatca taacgattgg ctatttgatt gtatcaaatc taggaaatat
     7441 ggtatttggt atcagtaaag aattcagttg gactactaca ttatgggaat ctctattctt
     7501 tttcatcttc gtatttttgt tacaaaatta tcgaaaaaaa taaacatgtg ccatctatct
     7561 gttggcacat gttttaggat ttatggtact ttttattcgg caatccattt tatttttcta
     7621 cttcttctta atacctctaa cacttttgat ttataaacta atccaatgca tctacttgcg
     7681 cttattaagc tatttcagct agatagtacc accccatgcg tcaatgcttg attttccttt
     7741 tgtataacat tgtataagca ataaaggaca aataagtggt gattacacca aaccattgaa
     7801 taagaaaaat tctgatacgt aaataaacat gaacttgaaa aaattaatga atcggttagc
     7861 tactacgtag ttgtcttttt tctcatacag ttctgttaac ttacacttct cttcgctaga
     7921 taataaaaaa tcctccaact gtattgtcca atataatccc aggcatctaa actagcatta
     7981 tagaagagac acacatagaa tctaaaataa ctggttttaa atgtttctta caatcaaaaa
     8041 aagctgttaa
//
LOCUS       DAFGZE010000105         7737 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.109, whole genome shotgun sequence.
ACCESSION   DAFGZE010000105 DAFGZE010000000
VERSION     DAFGZE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.109"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            190..1617
                     /locus_tag="LTU54_002708"
     CDS             190..1617
                     /locus_tag="LTU54_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297361.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1709..2074)
                     /locus_tag="LTU54_002709"
     CDS             complement(1709..2074)
                     /locus_tag="LTU54_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL8297362.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(2071..2436)
                     /locus_tag="LTU54_002710"
     CDS             complement(2071..2436)
                     /locus_tag="LTU54_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HBL8297363.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2628..2876)
                     /locus_tag="LTU54_002711"
     CDS             complement(2628..2876)
                     /locus_tag="LTU54_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297364.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2875..3003
                     /locus_tag="LTU54_002712"
     CDS             2875..3003
                     /locus_tag="LTU54_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HBL8297365.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3060..4754
                     /locus_tag="LTU54_002713"
     CDS             3060..4754
                     /locus_tag="LTU54_002713"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HBL8297366.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4758..4877
                     /locus_tag="LTU54_002714"
                     /pseudo
     CDS             4758..4877
                     /locus_tag="LTU54_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            5405..6538
                     /locus_tag="LTU54_002715"
     CDS             5405..6538
                     /locus_tag="LTU54_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBL8297367.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6569..6889
                     /locus_tag="LTU54_002716"
     CDS             6569..6889
                     /locus_tag="LTU54_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HBL8297368.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6918..7382)
                     /locus_tag="LTU54_002717"
     CDS             complement(6918..7382)
                     /locus_tag="LTU54_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBL8297369.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7514..>7737)
                     /locus_tag="LTU54_002718"
     CDS             complement(7514..>7737)
                     /locus_tag="LTU54_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297370.1"
                     /translation="VSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAA
                     VNLVLSILIYLPFVKVATIQERRKEALDA"
BASE COUNT         2535 a         1481 c         1381 g         2340 t
ORIGIN      
        1 tttttatatt gttaaaaatt cgcaatttat tttttaatag aattatctgt tttccaaatt
       61 ttataacata aaaaaccttt tttaacggca tttatatgct ttttcagaaa ttaactgatg
      121 atttcaaaaa gaaaacatgt cataatattt cccatattgt gcaaaaaaat acaaggcgga
      181 ggctctacta tgattgaaga atatattgag aaaaatattt taagacagct gtttttatgt
      241 gggcaattct atgtaaataa agaagtcaat ctagaaaagc tctccaatct cctgcatgtt
      301 tgcaaaacca ctttactcaa tgatatcaat aacataaaaa aagaatttga agaacagatc
      361 gcttatacac atcgggaaaa ggaccgttac actttatatt tttcagaaca tatacctcga
      421 tgcaagatca tgcagcaatt gtctcaaaat tcgttgtttt taaaaacttg tctgctttat
      481 ttggaagaag atgaacctga ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc
      541 aaagcttatt cattaaaaaa acaagtatta gcttatttta atgactgcgg gatcgaaatc
      601 gaccgctact cccctcgatt cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg
      661 tatcgtttag gcggttttaa ttcttgggag ctacctgaat cgttttttga acgtgccgat
      721 cgtttcattg aatccgtaac ggaaaatagt ggcagatttt atgataaaga aaataaagaa
      781 atcttatcga ttggttttgc tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata
      841 gattcaaaat ttatcgaaga aatcaaaaaa cgtcctattt acaattatgt cgaatcagca
      901 tgggaaaaca ctgatttcca aacctattat aaaaaagaag aattcgcctt tattttgaca
      961 ctattcaatc tttgtaatta tggtttccat tcttatcagt tgattgcgga agactttcaa
     1021 cagctacacc aagtgtttat tgacaatact cccgaaataa aagaactagt tgctaccttt
     1081 gaatctcact tcaatcaaga attattcgga aaccaaccgt tcgaacgtgc gctgattcat
     1141 ctgatgcgtt cggcttggga taactatcaa ttattcatgc cagaaaaatt ttatctttta
     1201 aatgaggaac agacaaattt atataaagaa gtccagacaa ttttttccag ctggtccagc
     1261 cagttacctt atgatctacg gttgaatcct aattgcatgc gggcttttgt gatcgaattg
     1321 agcggaattc tccgtctgac aaaagaacat ctgactatct atatcgtcac aaattcggat
     1381 gttcattatc tgatttatcg ggaagccttg gaagctgtaa ctactttcga ttttcaagtt
     1441 gcaccaacaa tttattcaag tatatctgat ataaagaagt atgcgcagca gtcctcaaac
     1501 cgggtattat gtgaacgtac attatataca ccagatgcgg tccaatatga aaatattatt
     1561 cctatttcga tcaatacgat cgatcgagct atcatttcag ctgtgcaaaa caaataaggg
     1621 gctgtgacaa aaatcatgtc acagcctctt atttgttgaa gatgcccatt tctgtaacaa
     1681 tctcttgata cacgtcaggc tcacaacctc acttattttg gacatctacg ggtccgatct
     1741 ctttatttaa aaaataagaa atgatggttc ggataactac caacgccgct agttggaaaa
     1801 tatcctgcaa ggtaggtttg ataatcgatt cgatgatccc agctgaaaca agaatttcca
     1861 aacttagaag tatatagcct cccaacatct ttttaatagc attgttctcg ctgactcggt
     1921 cgctttgcac ttttgtatgg acaaataaat atttcaaaaa atccttgatt gatagacaaa
     1981 caccccaaac caataaacag atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat
     2041 atggaagaag aaactgcatc aactctgtcg tcatcctacc tctcctcttc tttgtttttc
     2101 tttgtcttct atttcctgat tcaagaaata agaaatgact gttcgaatga taataataag
     2161 tgcaagcttc aaaatatctg tccaagtcgg cttgataact gactcgatga tatcagcaac
     2221 gactagtact tccaagctta acaaaatata acttgctagc agttttcttg tatctgcatt
     2281 acgagtatta cgttctgcat agtttttctt gacatgagac caatcaaaaa gtttgaatag
     2341 aacaagaacg atcccgacaa ttaagatacc gatagaacaa atatttaaaa tagcaatgat
     2401 ccattctaga aattcaatta aatttgagcg aaacatatac gctacctact ttcttttaat
     2461 ttgttcctat tcttttattg taaaagaaag ttaccttgtt cgtctattta aaagttgttc
     2521 taatgataac ggttcgctta aatttttctt tttcactctc ccatttttct ccctccatcc
     2581 ataaatcgtg gaagtatcca aaacaacgta gcaagttaaa ataaggctta gtccgtcatc
     2641 agcttttcaa agaagcactg tctcggtgct ttttttgtac ccaaatttct aaaaatagaa
     2701 ccatacacct cctcaaatct tttcttctta cctaccctat ttttatttac aggaaaatca
     2761 gacacttctt cttttttgtc aaaatttcct ttttcttctc gtcctatcat ttggcattct
     2821 aataaaaacg aactatcctt ggatatagaa agagaaaaca aggaggcgtt tttcatgagt
     2881 aaaaaattaa tcaaagtcgg catcggttta ggtttgcttg cccttggcgc tgcatatcta
     2941 ggaaaaaaga caggactatt cgaagatgac agtcatttat atgatgaatt cgaatcaatc
     3001 tagcatccta tttagttgaa tcactacgga catacttgga agacattgga ggaatcacaa
     3061 tgagaaaaag taaagcaata atgattggtg ccggtattgc caatatggca gcggctgttt
     3121 atttgattca agaaggcaaa tggaaagggg atcagatcac cttttattca ttggatgatc
     3181 acggttcaaa tgatggtgca ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc
     3241 atccattaga aaaccaaaaa ggctatattg ctcgaggcgg tcgcatgctt aattaccgta
     3301 cctatgtaga tttgatggat ctgttaagtc gaataccttc tgttactgag ccagggatga
     3361 ctgctgaaga agatacgaga gattttgatg caaaacaccg aacctttgac aaagcacgtt
     3421 tgatggaagg tggcaaagga atcattgatg gcggaaaact gggattcaat aataaagacc
     3481 gtattcttct gactaaactg attgctatgc ctgattcaga agaagaaaaa ctagacaata
     3541 tcactatcga agaatatttc aaagatgatc cacacttttt tgaaacaaac ttctggttta
     3601 tgtgggaaac aacatttgct ttcagaacac gtagttctgc tcaagaattg cgtcgctata
     3661 tgcatcaaat gatttatgaa ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac
     3721 gttacaatca atacgaaagt attatgcttc ctttgatcga gtacttgaaa gaacaaggat
     3781 gcaaaatcat tttgaaccgc cgtgtcactg actgggaatt caaagaaaca ccgatgcaag
     3841 atgaaatcac tgtgactggt ttgaaaatta ccaatacact gactcaagaa gaagaatacg
     3901 ttgtggtcga tgaagataca gcagttattt tcactaatgg gtccattaca gattcagcta
     3961 ctttaggaga ttacgaaaca ccagctgtag aaaatatgga ttatggtgca gcttctagtc
     4021 tctggaaaaa agccagtgaa cgtttctaca acttgggaaa tcctgacaaa ttctttgctg
     4081 atcgagatgc aagcgaatgg gtcagcttta ccctgacgac gaaaaaccat ttgttgttga
     4141 atgaaatcac ccgtatcacg actcaagaac caggaaatgc cttgaattca tttatttcaa
     4201 ccacaccgat cacgccgtta ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc
     4261 aaccgcattt tacgtctcag aaaccaaatg aaacagttat ttggggttat ttcctttatc
     4321 caagacgacg tggcgaattt gtggataaac agtatattaa aatgaatgga aaagaaatgc
     4381 tagaagaact gattggacaa ctttcaaaag tagatcctgg accagtgaat attcgtgaaa
     4441 aagaaacaga aattttcgac agcgtaataa ataatatccc tgtctatatg ccttatgctt
     4501 ccgcgttgtt caataatcgt gccaaaagcg atcgaccaaa agtcattcct aaacattcga
     4561 ccaatctggc ctttacagga gaatttgtcg agcagcctta tcagatgatc tttactgaac
     4621 aaagtgctgt acgttctgga gaaatcgctg ctttccattt tgcgggtatt cctatgtccc
     4681 gtcttgtcaa aacaccgcgt tacgacaaag atattcctac tctagctcgt gccgcaaaaa
     4741 aaatgtttga ataatcaaga ggttgtgaaa aagctgccct gcatcaagta ataagaacaa
     4801 ccaggctaaa acagcttctt atgtttttga tttggttgga cttattaccg cagatgcaag
     4861 cttttgaaca ccgtttacca agaggttatg aaaaagctgc cctgcatcaa gtaataagag
     4921 cagccaagct aaaacagctt cttatgtttt tgacttggtt agacttatta ccgcagatgc
     4981 aagcttttga acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa
     5041 gaaaaatttt gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa
     5101 actgatccct tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta
     5161 ttttttcaac gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa
     5221 tgattaccat tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact
     5281 atagaatgtt tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta
     5341 aaatgaaatt atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc
     5401 tccaatgaaa gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga
     5461 tccaaaaata ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg
     5521 ctccgatctg catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg
     5581 acacgaacca atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg
     5641 gcaacgtgtg gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca
     5701 catggaaagc caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt
     5761 tggcaagttg aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc
     5821 agactctact tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct
     5881 atcagatatt ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga
     5941 tacagttgtg attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat
     6001 ggctggagca gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc
     6061 taattacaat ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt
     6121 gtatgaactg gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt
     6181 agagcctgtc aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc
     6241 attacagatg gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta
     6301 tatgactcca gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa
     6361 gcatggacag gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg
     6421 actattcgat cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc
     6481 atatgagatc tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat
     6541 tcagtaaaaa atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat
     6601 cgaaatagga aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca
     6661 aaatcgttac acattgatct cactggctgt cgatttatta accgggattt tctatatttt
     6721 tggaagcctt gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc
     6781 tggtgctatt ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta
     6841 cgacgaaaaa aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa
     6901 atctcatgcc tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca
     6961 ctttcttcct tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga
     7021 tctgtaatta agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct
     7081 aatatccgct tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga
     7141 tttggtgtca gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg
     7201 atggtttctt tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt
     7261 ccagtacgaa tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa
     7321 ttcgcgcttt gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc
     7381 aatgtttaaa cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa
     7441 attagcaatg acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc
     7501 gttttttgat taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct
     7561 tgacgaacgg tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac
     7621 ctgcaatact ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca
     7681 taacggtagc tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaacta
//
LOCUS       DAFGZE010000106         7599 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.110, whole genome shotgun sequence.
ACCESSION   DAFGZE010000106 DAFGZE010000000
VERSION     DAFGZE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.110"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(942..1355)
                     /locus_tag="LTU54_002719"
     CDS             complement(942..1355)
                     /locus_tag="LTU54_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HBL8297371.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="LTU54_002720"
     CDS             complement(1811..2296)
                     /locus_tag="LTU54_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297372.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="LTU54_002721"
     CDS             complement(2435..2650)
                     /locus_tag="LTU54_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBL8297373.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="LTU54_002722"
     CDS             2874..3674
                     /locus_tag="LTU54_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8297374.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="LTU54_002723"
     CDS             3693..5561
                     /locus_tag="LTU54_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBL8297375.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="LTU54_002724"
     CDS             5554..6045
                     /locus_tag="LTU54_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBL8297376.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /gene="srlA"
                     /locus_tag="LTU54_002725"
     CDS             6033..6587
                     /gene="srlA"
                     /locus_tag="LTU54_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBL8297377.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..>7599
                     /locus_tag="LTU54_002726"
     CDS             6605..>7599
                     /locus_tag="LTU54_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBL8297378.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1213 c         1443 g         2236 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctg
//
LOCUS       DAFGZE010000107         7532 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.111, whole genome shotgun sequence.
ACCESSION   DAFGZE010000107 DAFGZE010000000
VERSION     DAFGZE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.111"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..865
                     /locus_tag="LTU54_002727"
     CDS             <1..865
                     /locus_tag="LTU54_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297379.1"
                     /translation="IVVPKNKSEDGNYELEVLQFKIEQKSSERFMSNQLVKIKKWLPM
                     IDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFANSFWFLKNINKTVEKNGVYK
                     GDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSK
                     SNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERD
                     SYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKI
                     YKEIIEQYFPK"
     gene            1048..3723
                     /locus_tag="LTU54_002728"
     CDS             1048..3723
                     /locus_tag="LTU54_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBL8297380.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3905..4144
                     /locus_tag="LTU54_002729"
     CDS             3905..4144
                     /locus_tag="LTU54_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297381.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            4160..5533
                     /locus_tag="LTU54_002730"
     CDS             4160..5533
                     /locus_tag="LTU54_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297382.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5537..7171
                     /locus_tag="LTU54_002731"
     CDS             5537..7171
                     /locus_tag="LTU54_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297383.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7334..>7532
                     /locus_tag="LTU54_002732"
     CDS             7334..>7532
                     /locus_tag="LTU54_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297384.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2698 a         1160 c         1490 g         2184 t
ORIGIN      
        1 gatagttgtt cccaaaaata aatcagaaga tgggaattat gagttagaag ttttgcaatt
       61 taagattgaa caaaagtcca gtgaaagatt tatgagcaat caattagtga aaatcaaaaa
      121 atggctccct atgattgatc aattagtatc taataataag gaagctaaaa atttatgtat
      181 ttttgtctta ttattacatg gaagagttat taacttagtt aatcgaaaaa gtacgactaa
      241 atttgcaaac tcattctggt ttttaaaaaa tattaataaa acagtggaaa agaatggagt
      301 ttataaaggt gattttcatt ttcaaaatgc tgagactatt gaacgagaac atcattattt
      361 cgatttatta gtgaatacag tggtagaatt agcgaaatgg gcaaagtcaa acgaaactta
      421 tagtcaagta cttaaaagaa ttatgagtca agaaacttca tttatctatg agttaagcaa
      481 gtcaaatttg aaatatactt ataatgaaga tgaacttgaa attttagata atccagaaaa
      541 ttcacaatgg attaaagagt tgaagagaga agactttatt gagacactaa gaattctaaa
      601 atcgttattt gatttgaaat tagccaaagc taccatcatg ggaattgaaa gagattcata
      661 ttattgggag gaaattggac tatatgctcc ccagattgaa ggtgaagata aagagaaaag
      721 gatagacaac attattaact atattaatag tttaagtgaa cctttaaaat gtagaggact
      781 tgattttata ggaacattag gatggacaga aacatactat gagaaaatat ataaagaaat
      841 aattgaacaa tattttccta aataatttaa taaggattga tttatcgtaa aagaaaaaag
      901 ttgtgatttc caaaatcaca acttttttct tttgtcaaga ttttgagtgg gatatatttt
      961 tgtagaactg aagatcatga aattatgaga ggttggaggt aatgtagtca tacaaataaa
     1021 aattttttag aaagtgagtg gtaattaatg aaaatgaatc acacttcaat tcagaagcat
     1081 ttacaactgt taaatacact ggggatttct tatgttaatt tgagaacttt ttctacatta
     1141 aacaagaaaa ccgtttctca tattttttct actgcagatt ctcaagcaat tattaactat
     1201 ttagaaaaac agaaattgtc tgaaggtaca gtaaacataa cttataatcc tattaaacaa
     1261 gaggtgatta cacaaaaaaa gcattctgtg agagacaatg atattgaaaa aattcggttt
     1321 gtatttattg atattgaccc taaaaggaaa gagggaagtg caacagatag cgaaaagaaa
     1381 aaggcagaaa atgttatgga acaagtagaa cgttatttga aagaaaagag aatagaatca
     1441 tttgtaaaag ttgatagtgg gaatggctac catatttttc ttcccataaa tgaacaacca
     1501 aataatcacg agacgattct tactattcag aattttcttc aattacttca ccgaagattt
     1561 ggagtagaag atgaagttga tattgatact tctgtttata atccttcaag attatgcaaa
     1621 ttagttggta caccagcggt caaaggaaag tccacaaaag agcgtccgca tcgtttatct
     1681 agtttccttt ctattcccga taacatagat agaagtaatt gttttaaaat tcttgaacag
     1741 atagttgaag aaaattccat gatgtcaacc tctagagtaa aaagccatcc atcttttaga
     1801 gaagtgttac cttttattca agcagactat aaacaatggt tagctcacta tcctgaatta
     1861 acttatacca taaaagaggg cgatcataaa ggagttacct tacttatatt tgacgaatgt
     1921 ccattaagaa aacacacgaa taattctaat ggttcttgtt tgtctgttac ggctgataat
     1981 aagattcgat ttcattgtct gcatgcttcg gataaaaata aagatattca agactttgta
     2041 aaaaaatatc cgttgccaaa acctgtaaat ccttctgtag aatcattaga atccaatcaa
     2101 gaatattatt ttgaccagtt taaactgaat caagaaggag tatttttggt gaaagaaggt
     2161 caagaagaaa aagttgcttg tccgatgttt attaagcaaa tacgcaaaaa tatcgaacaa
     2221 aaagtcatta cacttgttct tgcctattat gtcgatcatt gttgggatga aatagaaata
     2281 gaaggtggat gtttacaaac taataatttt aaacaattag caaaacatgg cttaatcatt
     2341 aaagctagaa gagaagctga agtcgttgac tttttacaga ttcaacggga gaatgctcca
     2401 atcttttttg aacatgaatc tcttggctgg aaaaaagaaa atgaagcaat gatttttcag
     2461 ttacaagatt cttttggacg tgaaattcct tctgtattaa ctaaaaactc atgctaccaa
     2521 ttaacaacta atggtacaga agcggaattt gacgcattgg ttcataagta tgttttagga
     2581 acgaacttag aacttgcttg gtcgattggt attgcttcta ttgtgttagg ctatttaaac
     2641 gttacgaagg ctaatacttt tttgagttta ctaattaacc tacagggcaa gagcacaacg
     2701 ggtaaaacca ctacgttaca ctttattgcc tctttatttg gaaatccaac tacggttctt
     2761 agaaatatga atgctaccaa taaagccatt ataaagctag catcaaacaa tcagggaggt
     2821 gttccactaa tactagatga gttaggagca gcaactcaaa ataactttag ctcgttgatc
     2881 tatcaattag cttcaggaga agaaaggcta agattagatt ctaattgtca gcttatgcca
     2941 caagagactt tttctgtttt gacccttacc tcttctgaag agcgtttgga aaattatttc
     3001 aatgagaaat taactggttt gaaagttcga tatatcgaat tttctgatat caagtttaca
     3061 aaggatgcga atcacgcaga gaaagttaaa agtctttctt ctaatacttt tggttggggt
     3121 ataaagttat ttcttttgaa actctttgaa caagggatta caaaaattga agcaactttt
     3181 gtcgaagctc aaaaaaaact atgggctgtt ttacctgaac attctttgaa gcaacgcatt
     3241 ggtaataatc tagctttgat ttatagtgga gccctcctaa ttcaagatat actgggctat
     3301 cctttacgat tagagcatat acagacggtg cttattaatt cgtacgaaca gttaattgta
     3361 gatactagtt taggaagtga gcaaacggta tttgaccgag tgaaaacatt gttggtgatg
     3421 aatagccata aatttttaac aaacagtgac aacaaacata cgcccagtac gatttgggga
     3481 aaagtatcaa ttcaaaaaaa tggggatatt aaggtaaata tttttcctat cccgtttgag
     3541 gacttgttaa ggcgagaatt tcaagtaaag gatctaacct atctttttag gcaattgctt
     3601 gattcgggta atatgtgtgc agaaaaaggg agaagaacga aacgtattca catgaatcgt
     3661 caagttcttc caacatacga gattctatta ccttcttcac tcaaatcgta ttttcgattg
     3721 taatttagaa taaaccaaag tatgaaagtg aggcaccatg ttatggaaat atttcttatt
     3781 gatttagcaa aacaactgat tacttatctg tatgatagcg agcaaaccca aacggaagta
     3841 aatgaagctt gatctatgct tcatttactt cattcaatca aacatgaaag aaaggaaaat
     3901 tctaatgact aaaaataaaa tgacactaaa agcagaggtt cttctgtata ttcaagaaca
     3961 tttttcaaat caagcctttt ttacaaaacc aatttacttg gatttcgaaa taagaggggt
     4021 atctgctgga tctatcgggg gtactcttca agcgttgaag aacgaaggat atttagaaaa
     4081 tcactttgtg caacgttcgt tcaatagaag agtcgtgaaa aaatggtatt tagttcattc
     4141 atagagaggg gaatcaagca tgcgtgtggc ttgttatgat cgaacgtctg tttatgaaga
     4201 agcgaaaatt agtaatgacg ctcaatatga tgcagtgaaa gagtatgttt tagagaaata
     4261 tgaacaaatt ccccaacgat tttcagacaa aggagaatca ggggaaaaag cggagcgtcc
     4321 agcttttcga cagcttagag cttgggtttg tgcgtcagaa gaagaggggg tgatctatgt
     4381 ttggcgttat gatcgattgg cacgtcaagc caaaatagcc ttagaattta ttgagttgtg
     4441 ccaacaacat caggttcaag tgtattcgat ttctgaacct cttcctgctt ctttctcaca
     4501 cgcgtcaacg aataaactat ttgtgcaatt attgttcact tttgcagaac aacaacgaaa
     4561 cgtcattatt gagaatgttc gtgccggact ttcctataaa aagcaacaac ggcaatattt
     4621 gtcttctgcg gtaccatatg ggtatcgttt agtgaatggg aaaatccaac aagaagtaca
     4681 cgaagcaagg attgttcgct atatttttca actttatttg accaaaaaat atggctacaa
     4741 aaagctttgt caacgattga cgcaacaaaa gcttttcttc cgcgaacgtc cttttcagcc
     4801 ctatcatatt tatagtatcc taaaaaatcc attgtactat ggcgaaatca aaggaggatc
     4861 tttggggaag tatctaggaa cctttgaacc gattctctcg aaagcaacct ttttacaagc
     4921 ccaagagatt cgccaatcaa ggtgtacagc taaaaaagat acgtatctgt acttattacg
     4981 tcaaaaaatt cggtgtccct tctgtggtcg tcatctgagt tcgaagtatc aatggaatac
     5041 aaagaagacg aagacgcttc attattatca ttgtaccgac cgaagctgcc aaggaatttt
     5101 tcttcgggca gaaagggttg agtcagaagt attggaaaag atacaggctt tttttcaata
     5161 cgaaggcgtc tatcaagcct tgattgccca attaacgcaa caattagccg aaaagcgaaa
     5221 agatgaaaaa cgcaaagtaa tccaacaaaa gaaacaagaa gcacaatgga ttaaggcatt
     5281 tgaagagggc aattgttcac tggaagaact caagcaacaa ctgcaagctc ttcaaaagcc
     5341 gatgtctact tcttcgtttc tttcactcca acagtgtgaa gcaaaagtga aagaatggct
     5401 tcaactaaaa gacctctcta gtcgacaatt attatgtgac tatgtttgtc ggattgaggt
     5461 aacaaaggat aaaacagttt gtgggattta cttccgacaa ctgacacaaa atattttaga
     5521 aaggaacgaa tgatctatgg aaatgataca aaccaaacga gtcgcattgt atattcgcgt
     5581 gagtacacaa gaacaagctt tagaaggaca tagtattcaa gcacaagaac aaaatggacg
     5641 acagtttgcc gaacggatgg gctatgaagt ggtagaagtt tatgccgatg aaggaatcag
     5701 tgggaaatcc acaaaaaatc ggttcgccta tcaacgaatg atgaacgatg ctcggaacca
     5761 tcgttttgat ctactagtga tttggaaatt gacccgtctc ggacgcaata tgttagatgt
     5821 cttacaaaca gtcgaggaat tattgcgata tgacgtagga ttacattcga tctcggaaca
     5881 atttgacgtg accacttcga caggtaaatt gatgttacaa ctccttggga gtttcggtga
     5941 atttgaacgc aaccaaattg cggagaatgt tcaaatgtca atgcaaagct tagtgaaaga
     6001 taaaaagcgt tatgcaggag gccgacgttt aggctatgtc agtggtattg acacgaatgg
     6061 tcaaaaacaa ttggtcattg aaccagaaga ggcgaaaatt gttcgtctga tctatgcaaa
     6121 atatttaggc ggagcagggt atcgagcgat tgccaatttt ttgaatcgtc aaggatatca
     6181 aacagtcaaa aagaatgctt tttctaccac ggcagtcaaa gatatcttac gcaataaaat
     6241 ttatgcaggg tatttagaat atgcgcgcta catcaattgg gaaaccaaac gacggaaggg
     6301 gaagaatccg agaccgattt tagtgaaagg aacccatgaa ccgattattt cggaagaaga
     6361 ttatcaaaaa gtacaagaac gccttgcttt agaaggaaaa caacccaaat ggaaccatac
     6421 aggtgaaaat gtattgacag gcttgttacg ttgtccagaa tgtggcgcac cgatggcagc
     6481 gagtaatgtc acaaataccc taaaggatgg cacaaagaaa cggattcgat attattcttg
     6541 ttcagtcttt cggaataaag gagcaagtgt gtgtcatgcc aattcgattc gtgccgaaca
     6601 agcggaaaca tttgtcgccg accgattaaa ggagatcgtt cagctaccag aagttcttcc
     6661 ccgattggtg gcagcgttaa atgaagaaat agtgcgacaa agccaaccat tggaacaaga
     6721 actcgtggtg ttactggaac gaaaggaaga attgaagact aaaattgaaa aatgggaagc
     6781 tgctttggaa gatagtcctg aattgtttcc gatgttaaaa gaccgcttag acgaattgac
     6841 tgaaaaacga cgacagttgc acattcgaga aaatgaaatc ttagggatct ttcaacaagg
     6901 agagccgatt caagtcaaag atgtccaacg gatattgact agtttggatc gttttcttgc
     6961 ccagagtgag aagaagcaaa tcaaagcctt gtatcgtacc tttatagaga aaataacctt
     7021 tgatcctaac cataaggaga tgttggaaat tacgatgaaa ttcacgcatg ccgtggtaca
     7081 acaattgaat gagcaatatc aaatagccgt atccaaaaca aaggatacgg ctatttttgt
     7141 gttaaaaaca ccctttacgt taacgatcta gtctaggtta tatcaaacaa cgttagaggg
     7201 gcgctaacgc ttctctagcg atttgaaatc gttagagaag tagttaggga ggtccttatc
     7261 ataaataggg atatttatta atgaatattt gaaatataat tgtatataag aattaaaaag
     7321 agtggggata gatatgaaaa agatgggtaa gtgtatttta tgtgaaaaat acactgaact
     7381 tactaaagaa catgtaccac ctaaaaaatc agggaatact ggaggtaaaa agaccagaac
     7441 tggtaattta gatgatttcc ttaagagcga ttttacaaaa ggtgattttc cgaaggggat
     7501 taaaaggaaa cctcaaggaa atgtttatta ta
//
LOCUS       DAFGZE010000108         7479 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.112, whole genome shotgun sequence.
ACCESSION   DAFGZE010000108 DAFGZE010000000
VERSION     DAFGZE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.112"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..770)
                     /locus_tag="LTU54_002733"
     CDS             complement(<1..770)
                     /locus_tag="LTU54_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HBL8297385.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANA
                     NKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKLKERKISKADPE
                     SGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIH"
     gene            complement(2083..2748)
                     /locus_tag="LTU54_002734"
     CDS             complement(2083..2748)
                     /locus_tag="LTU54_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297386.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2928..4910
                     /locus_tag="LTU54_002735"
     CDS             2928..4910
                     /locus_tag="LTU54_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBL8297387.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4914..5552
                     /locus_tag="LTU54_002736"
     CDS             4914..5552
                     /locus_tag="LTU54_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297388.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5580..6008
                     /locus_tag="LTU54_002737"
     CDS             5580..6008
                     /locus_tag="LTU54_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297389.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6465..7376
                     /locus_tag="LTU54_002738"
     CDS             6465..7376
                     /locus_tag="LTU54_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBL8297390.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2531 a         1168 c         1373 g         2407 t
ORIGIN      
        1 tcatgaatat ttctaggagt gatgattgtg ctaagaataa atccattgtc gtcgcatgaa
       61 gtatggacag aataagcaaa ttgtttttct cgttcgcctt tgacgtagta gccgctttcc
      121 ggatcagctt tacttatttt tctttctttc agcttcactt ccttttctaa gtccaaagta
      181 aaagggcgtt ttccagcctc tattcgttga cgattgatct catcttctaa ttcatcctga
      241 taggctttcg cttcatcatg ggtcatttct ttcgtaaact tatgtttatt ggcgttagct
      301 ttgatgtggg tagaatccag atacaaatta tcctctgtta caaaacctgc tttaactgct
      361 tgttctaaaa tataggcaaa aatatcctca aacaccgtcg tttctctaaa tcggcgaaca
      421 taattctttc caaaagttga gaaatggggg attttctcct cgaaggagta gcctaagaac
      481 cagcggtaag cgacattggt atcgacttcc ttaatggttt gtcgcatgga acggataccg
      541 aataaatact gaataaacac caatttgatc agaatcactg gatcgatact tgggcgacct
      601 aaatcggagt aagtggcttc gacgattgga taaataaatt caaaatcaag cgcggcatca
      661 atctttcgaa cgagatgatc ttgaggaact agatcatcaa tcgtaagcat cgcaaattga
      721 tttctaccat tcgtattgtt tttagacatc atagctatca ctccttgcat tcgttttcat
      781 aaatctatta taaaagaaaa gactgtagat atcatccata aaatggactt tgtctacagt
      841 ctgaggacta cctgtattag tcctcttttt tgaccagatt ttgaccagtc tattttacat
      901 tctaatatat tcatatgagc taattttatg attaaatggt aagtgtttcc ttgtttgttt
      961 tagtttaaac tagtgtgtaa tttaaaaatc tatatggaag ggtctgtggg gtaatactta
     1021 aataaaggct aggaacctga taacatcaag gttcctagcc tttttataat aaaatgaagc
     1081 gaaaataatt tcttttaaag agccgagatt atcagctata taagagtgaa tttgatgcaa
     1141 aaaacagagc cgaaacagtc ttggtgttcg tagctctgct tatatgtttc gaatttaaga
     1201 caaagagttg gcaaaagatg ttgataagga agataagata actgtgttat ataaactagg
     1261 taactaactg taacaagcta attttaagag attcttatta gaagacgttt aggaatataa
     1321 ctaagaaaat gattatacag gtaattgtca tttcaataat gttttctaat tttcgcgtct
     1381 ttgttttaag aaaaaggcca gaatcagttg gaaaaaagtt cgtacgatag tgaagagggg
     1441 caaaatttcc tactaatatt cccactaaca agagataaaa cggccaggtt ggtgtgattc
     1501 ctctataaaa aaatgcccat gctaccgaaa tcccatagat taacaactga acaattttta
     1561 caatgaaaaa attgttctgc cattactcaa agtctcttcc tccttgtatc gtttatttgt
     1621 ttcattttat cattatgata ggcgcttgaa caatccattt tgtattttat tgagtactct
     1681 tggataaact accagcgatt atcatattta tttatgttac tgtaagccat catataaaag
     1741 taaatgagca gatcatagaa atataaaaaa gaactaaaat tgatctaaat tatgtgcagc
     1801 ttgactacgt accaatctat attacttact agattggtcg ttaatctatt cggatcgaat
     1861 gagcaaaata tttttgtgaa tgtccatcct tgattatgat tccaaaaaga atcattggga
     1921 ggctgtggcg taaatattat actataagcc agaaatacct atcattctga acttttttat
     1981 ttaggaactt tcatctcaag gctttttcaa ttaatttgta cccctgtttt ttggataaga
     2041 tcatgtgcat tgattttttc tatctctacg ttcatctcct acttaatgcc tatctggctc
     2101 ctgagggctt ctttttaagg cgtcgtcata gtcttggctg aggatttctt ggtaatctga
     2161 gggaatggct tcccaagtga gcaggcgaac gttcatgacg tgttttcctt tgtgtttgat
     2221 tacagcatgg tttttctctt tttctacctc tccagcgtga aaagacagcc atgtaccatg
     2281 gccaaagata tcattacata cccaacgttc gatggaattg tccacgtatc ctacagggag
     2341 tgtggtatag ccgatttgtg gtttcaaaaa aggattgaat cgatcgatcg tttcattttt
     2401 gtattgataa ttgataagaa aaagatagac caaacaaaag acgagcagaa agaaagatag
     2461 tgtctttttg agtgagaagg gttgttttga tgaataagat ttcttgttaa caagaggttc
     2521 atctggcaat gttggttcta ttgattcggt agctgattca atagaaggaa aatcaatcgg
     2581 cagacctaaa gtatgatgta aaagtgacag catttcaagg tctggcattg tacgagaatt
     2641 ttcccaacta gaaatcgttt tatcggaaac accaattttt tcgcctaatt ctttttgcgt
     2701 aagcttacaa tttctgcggt aagttcgtat tttttctccg gtgttcatct tcttctcctt
     2761 tcgattctac agtatataga tttattctac atgacctata cacttgatag atacgttttt
     2821 ttgatgaaaa acggtatgat tttatcaaag aatcatttaa gtaagggaga gtggtgataa
     2881 tcaaaaaaaa gttcacagtt ttactcacaa tcatagtgac cggtatcatg agtggttttg
     2941 ccttggttag tataaataaa caggtactct atcggacaaa tgaatttttc catcaaacag
     3001 atacttatac tccgattcga ttgccacaac attttattgg gcaagaagaa tatgagcagc
     3061 taatcatggt atttgaaaat gtctcatcag aaactgagct ttcttatatg aaaaaaacag
     3121 taaatgaatc caatgaagta gatacatctt ggaacttcaa gatacagcca aagcttgaaa
     3181 tgacattgta ttatcaaggg gatgtcaaat cttctcacga gaaggtaccc tattctaata
     3241 cctcctttcg cgtaacgtcc attgaagaat cgatcaaaat aaaagatttt gaaggtgaat
     3301 ttttcttgat gacgagagaa aaagaagcct atgaacggtt catttcacta ttcactgatc
     3361 gattcaatca agcatttcac actgattatg ccactaatat ttttgaagac tttgaaaata
     3421 gctattcgta ttcggttatt tcaaatacag acgtatataa ttatcgagtg tatttaacta
     3481 ttggattgat tcttttattc cttttgttat catccttttt cttattgatg aataaagaaa
     3541 tcagtttatt gttgaataat gacttttcat tacatcaagt attgtggtgt ttattgggga
     3601 aaaagaaaat cattattaca ttcataattg caggaatcat tggtggtttt atttttatgt
     3661 attataagga gcaatttctt tatttgttcc agatgtacgt tttcttgttc agcggaatat
     3721 atggtatctc tttgatttct cttttattag ttgaaaaaat cgcttcgtta agagaagcgt
     3781 tcaactatat ccaatacatt ctttttacat ttggattgtt gttttctctt cttttgaccg
     3841 ttgccaatgt cgaattagca actatcgttt tttcttcttc acagttagct gtttcccatg
     3901 aaccaccaaa agaaatcgct ggtgaggact atcatgtatt ttatccagta accatcggga
     3961 aaaaccacgt ggattttatt tattccaatc gttttgcatc agcagcagat caggaattat
     4021 atgaaacttt gaataaaaac ggaagtatat tagtcaatgt atcagattac ttgaatgaag
     4081 aaaatgaaca gtttggcaga gggataaaaa taaatctgaa ttatcttaag aaatttcctt
     4141 tgattgatgt ctctggaagg ttgatccaaa taaccgaaaa agaaacgcac cctgttatat
     4201 tgatccctga acgatataga catacagatt taaaaaatga cctggcgcaa ataacagaat
     4261 attatcaaga aatcagtgaa aaagaaccga aatttctatt tataaaaaac aatcaaccca
     4321 tttatacatt tattccttct atcccctgga tcgaacatta tccggttgtt gaaatactga
     4381 cattgaaaaa cagtatgtat ttggaaagaa atattctcag cggagaaatc tatccaccgt
     4441 taaagatcaa gataggctct ttgtcgaaag aaaggttatt tgagctgatt gaagaaagtc
     4501 agttacgtga taacttgcag ttatcatttc cgtatactca gactgaagag atagctgtca
     4561 aagtgttaag cagatcattt cattacttga tacagatatt tttactcact ttcttttctt
     4621 tcttcttgtt gagctatgtc atgttatgca tatattttgt ctacaattgt cggaaaatcg
     4681 ctatctttcg ttcaagtggt tattcgctct ttgaaacgta taaagatttc ttcatgatga
     4741 acctcatcaa atggggaacg acaagtgtaa tcttcctttt tcttatagaa agagagccta
     4801 aatatctttt taatattttt ttctttagct tcattgattt tattctttca gttgttttta
     4861 ttttgtctac agaaaagaaa agtcagttat tgctgatgaa tggaggttaa caattggagc
     4921 cgttgatagt gataaaaggt gcgaaaaaaa tcattcgaaa caaaacgatt cttcatttgg
     4981 atcgtttcac tgcggatgct ggggatcttg tgactgttgt tggagaaagt ggagcaggta
     5041 aaagtacgtt attgaatata ttaggattga atgatcagtt cagttcagga caattctatt
     5101 tgttcgatca agagacgaaa caattaactg ggagagagaa gcgcttgata aaaagaaata
     5161 ccatatcttt tctttttcaa gattttgggt tgattgaata tgaaacaatc gattttaatt
     5221 tagatattgg attgaagttt tcgaatttaa atgtttcaga taagcgaaca gcaaagtatg
     5281 aggcattgaa aaaagtaaat ataaacaaga agctaagtac gcctatctac acattatctg
     5341 gtggtgagaa gcaaagaatc gcctgtgcga gagtcttatt gaagaatagc aaaatcattt
     5401 tagccgatga accgactggg tcattggata aaagaaataa ggataaagta gttgaaatct
     5461 tatcgcaaca agcttcccaa ggtacgttag ttatcgtagt cacgcatgat ccatatctga
     5521 ccaaaatagg aaacaagaag atcatgcttt aaaaatcaaa aaggaaagga tgtcgtttta
     5581 tgaaaaaagc agttattatt tgtgcactag ggataggttt tgttggagga aacgctatgt
     5641 catctgaagt ggaagcaaat tcagttcacg gtgaaatcta ttacaaatct ttaaagccag
     5701 gagagattca ttttgatgct aaaggtaata ttattaagtc aaatttacca gtagaccggg
     5761 aaatttctag aacagcaatt aatcatagct ctggaagatg ggtttattat agtgatacaa
     5821 cttgtacgag aaaaacaggt cattctaacc actatagtcg tgtctataat cgtcacggtg
     5881 caaaagcgaa agttggaaac tctattcgat cagcaaatgc aacaaaagga gtatgggcct
     5941 atgcaagtgc gacaggaaat agaactgaca cattctcatg ttggtacaat ccaaccggtt
     6001 ggtattaagt atccaaaagt ttatataaaa tagaaaaaac atataatgaa acagcgttaa
     6061 aagttatttt tggtagtttt aatatattca tttttagttg tataaaaata tatcataata
     6121 aaaacaacaa aaaaatgtct ggaaaatcga acataaaagt gtctgatttg aatatcccag
     6181 cagcggaaga agaaaaacaa aatcgataac atttgttcta agaattagtt acgttaaata
     6241 cggaacgcaa gatagacttg aaaaacggta acaaaagaag tagtatatta aggtgtgatg
     6301 tgctacttga catttattct gctgagcgaa ctttctggat tgtgtagcct tatatctatt
     6361 caggacgtat cgagcagatt agtaaaggtg ttcttgatga ttttcatctt gaacaccttt
     6421 gtttttagca attcgcaaaa aacgaaaagt aggagaattg ataaatgaaa aaaagatttt
     6481 tagcacttgc catcgtacta ggcactggct tattgtcagg ctgtacgaat gccggtgaaa
     6541 aaacagccgt tagttataaa ggcggtacaa tcagcgaaca agaagtaatg gatagcttga
     6601 aaaaaatgca aggagcagat tccgctgtgc aacagttgat cgtttatcaa gtatttgaag
     6661 ataaatatgg agatgacgta tcaaccaaag aaatcgattc tcaatatgat caaacaaaga
     6721 aacaattggg tgattcattt gattcacaat tgaaatctgc aggttacaca gaacaaacat
     6781 tcaaagacag tatcaaacaa agtctagcct ttcaagaagg tctaaagaaa catatcaaat
     6841 tgaccgatga agatttaaaa acggcttggg aatctttcca tccagaagtg gaagctcaaa
     6901 ttatccaagt agcaagtgaa gatgatgcaa aagatgtgaa gaaagctgct gataagggag
     6961 acgatttctc taaattagcc aaagacaagt caactgatac tacgacaaaa gaagatggcg
     7021 gaaaagtgaa atttgattcc actactacaa cggttccggc agaagtcaaa gaagcagcct
     7081 ttaagttgaa agatggtcaa gtttctgatg tgatcacttc taccaatgct tcaacttata
     7141 ctacggaata ttatgtggtt aaaatggtga aaaaccaaaa caaaggcaac gacatggata
     7201 aatacaaaaa agaattgaaa gaaattgcaa cagacacaaa attaagtgac agtaccttcc
     7261 agaataaagt aattggcgaa gtattgaaag acgctaatgt aaaaatcaaa gacaaagact
     7321 ttgaaaatgt tttgagtaca ttcacttcag attctagtac agcaagtagt aaataagcgg
     7381 tataaataaa aaatatgaac tatttaaatc aataaaactg ttcatctaaa tctttttatt
     7441 agaactaaat caacagtagt ttaattacta cttgattta
//
LOCUS       DAFGZE010000109         7314 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.113, whole genome shotgun sequence.
ACCESSION   DAFGZE010000109 DAFGZE010000000
VERSION     DAFGZE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.113"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(25..849)
                     /locus_tag="LTU54_002739"
     CDS             complement(25..849)
                     /locus_tag="LTU54_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBL8297391.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(822..1502)
                     /locus_tag="LTU54_002740"
     CDS             complement(822..1502)
                     /locus_tag="LTU54_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HBL8297392.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1525..2463)
                     /locus_tag="LTU54_002741"
     CDS             complement(1525..2463)
                     /locus_tag="LTU54_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBL8297393.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2708..3571)
                     /locus_tag="LTU54_002742"
     CDS             complement(2708..3571)
                     /locus_tag="LTU54_002742"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBL8297394.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="LTU54_002743"
     CDS             complement(3713..6286)
                     /gene="mprF"
                     /locus_tag="LTU54_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HBL8297395.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6427..7233
                     /gene="proC"
                     /locus_tag="LTU54_002744"
     CDS             6427..7233
                     /gene="proC"
                     /locus_tag="LTU54_002744"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBL8297396.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         2230 a         1604 c         1262 g         2218 t
ORIGIN      
        1 tataaggctt cttttcttta taatttagtt attttttctt ctgaaggcaa ccaatattcg
       61 tttgatgata tttacaatca ggaacaaagc aataaagact aatgtgattg ttgctcctgg
      121 aggtgtatct agataaaacg agctagttag tcctgacagc atccctagaa atcctacaca
      181 aacactgata gcaatcacga cattgaagct tttgcctaat cgcataccga ttgctgcggg
      241 aagaaccatg attgcggata tcaaaagtgc accggcaatc gggatcatca cagcaatagc
      301 cacaccagta atcacattga acatcatcga catcaaatga acgggtagcc catccacgtg
      361 ggcggtatct tcatcaaaag tcagaacata catcggccgt ttgaataaaa agaacaatat
      421 accgatggca acgaataaga ttcctaaaaa gatgacttgc tcccatgtaa tggtcacgat
      481 cgaaccaaac agataggatt ggatacttgt tgctgaattc cctccgctca aattcatcaa
      541 tactaacgct agcgccaaac caccagccat taaaatggca atggagattt ctgaataggt
      601 tcggtagatc gttcgcagat actccaatat cacagctgct aatacaacga ctagcatggt
      661 cgtaagtgta ggattaaggt tcaaaaagaa tcctaacgct acccctgcta aagagacatg
      721 agaaagcgta tcagccatca gcgattgtct tctgatcact aaaaacacac ctagcatcgg
      781 ggcgattccg gcaatgaaga tagcggcaag aaacgctctt ctcatgaatt catatgaaag
      841 caacgccatt gagaatcctc ctttctcacc agtcggatct gtctgtccgc atactgtttg
      901 atatcttcat gatcatgggt gatcattaga actgatttcc catggacatg cgcactatgt
      961 cggagaagac gataaaattc atttctggat tcttcatcca tacctgttgt tggttcatca
     1021 agaacaaata agtcagggtc agtagcaaaa atacgcgcta aactgattcg ctgcttttgt
     1081 ccgccagaca attcacctat tcgccgattt cgcatatccc acatccctac agcttctaaa
     1141 gcacgctgta catgttgatg atctttttca gagagacgct tgaaccagcg gcctcttgga
     1201 tatcttcctg aacggactaa ttcgatcacc gtactaggaa aacctgcatt gaaagatgca
     1261 acttgttgag gaatataacc gatacttaat ttttttcctt cttgattttt ctcagcaatc
     1321 tttatcattc cttttgaagg tttcagtaat cctaacgatg ctttcaccaa ggttgacttg
     1381 gctgctccat tttctccagt taaaatgaca aattcacccg catctacatg ataagagaca
     1441 tcttctagta caggttcatc atcatagtaa aaggtcaaat cagatatttc gatataacgc
     1501 accttacaac ctccattcta aaacttaatt gatactttct tttaaagctt tcagattttt
     1561 ttccatgaca gaaatataat tttcgcctgc tttgatttgt tcattagtca agctttctaa
     1621 aggattcaac acttctagct tcactcctgt ttcattcgac aatgtctcag ctaccttcga
     1681 agaggcattt tcttcaaaat aaatcacttt gatgttgttt ttcttcacaa attcttttag
     1741 ctcagctaag cgacttggtg tgggttcctg atctggtgaa acgcccgaaa tcgctacttg
     1801 ttttaagtcg tattgattcg ctaaataagc aaaagcagca tgctgagtga caaatgtgcg
     1861 gtttgttgca tcttttagct catcggagta tttttgatcc agtgcttcaa gctttttgat
     1921 ataagcatcg gcattcttcg taaaagtttc ctttttatct ggatattttt cgcctaattc
     1981 ttcagcaatc gttctgactt cttttatagc taataccgga tcgagccaaa catgtggatc
     2041 gtattcatgt gaatgaccat gcccttcttc tgattcttca tgttcgtctg cttcttgcga
     2101 ttctaaacgg tcgattcctt tagctgcttc gatcatcaag gtctgtttgc tatccactga
     2161 atcctttaac gaatccacaa aaaattccat gtctgaacta ttatacacga aaacatctgc
     2221 atcggaaatt tctgctcttt cttttgcact tggttcgaaa tcatgcggct ccgttccagc
     2281 agggatcaaa agtttcacgt ttccttcatc ccctacgatc tcttttgtaa aatcatacat
     2341 cggataaaaa gtcgtaacga tcgtcaaatc ctcttttttg tctgcttcct tgttcgtatt
     2401 tccacatgct gcaaacagta aagcacctat cattaccgta gctgtcaaaa gatatttttt
     2461 catttatatg ctcctttcaa tgaacaaatc ggaattttac gatttattaa cgtgaactaa
     2521 tgtatcaaaa taaaaaaaca cagtcaatag aaatccatca atttctgatt taagcatgta
     2581 tattcttgat taaatcgtaa aatggatgag ttgctgctca tatctagcca tttcactaca
     2641 ataaaaaact gaagaatacc attttaggat tcctcagttt ttcatcacct attttacttc
     2701 ttatatttta ttgcagggtt cgtactaaat aaacttcctc acaaaaacct tttaacccat
     2761 tatatactct ctgcgctctt gaataatgtt gacacaaggc aaaaacggtt ggtccgcttc
     2821 cactcatcaa tgcagcatct gctccgtact tgatcatacg ctctttgatt tgatggatga
     2881 ctggatgccg ctttcccgtt atatcttcta aactgtttcc catttttttg atcatctgct
     2941 gataatcttg ctctcggatg gcttcagcaa gtccgtggat gtcttgatgt tggatggctt
     3001 gcgtatctac ttctctaaac actgtacgtg tagagacact cattcttggt ttgaccaaca
     3061 caacccagca ctgtggcata gaaggtaata cttggatttc ttcccctttg ccaccgacaa
     3121 atgccgtact tccgtagaca cagtaaggaa catctgtacc gacttccaaa ccgatttctg
     3181 ccagttcttc taacgaacag cccagattcc ataattggtt gatccctctt agagcagctg
     3241 cacagtctgt acttcctccg cctaatccag ctgctactgg aatattcttc ttaacaaaga
     3301 tcttgacacc ggttttgata ttgtatttat tttttagcaa taaagcagct tgataaacat
     3361 tgttccgttc atctaccggt aaaaatgctt tatttgtttc tacaatgatt ttgtcttctg
     3421 ttatttcttc aaaaaataga tgatctgcca agtctacgct cgacatcacc atttccaact
     3481 catgataccc atcttgccgt ctatataata catccagtcc taaatttatc ttggctggcg
     3541 ctttttcaat caattccatc ctctcaccca ttcgaaaagt ctaagtcgca agtattctat
     3601 catagggaaa aataaaagta tagcttatgt ctgcctagtg gttgattttg tcatcgtttc
     3661 aacagctttc acagaatttt ctcttcataa tccaaacaag ttctagtgat tcttataatt
     3721 ctgtgagcac ttcttctgcc aaactcggaa catttccttt ttcttttcct atcagactga
     3781 gtagctgcat ggtagcaaat ccgaaattac ttcctttcct ataagcaaaa taacgtcctt
     3841 cccaatgatc gacatatttt aatttctttt cataagcaga ctggaaggaa tagaaaaaat
     3901 taccataatt atatacgata tgcatgactt tttcttgaaa gaaagaaaaa tctgtttctc
     3961 caacattagc taatggagct aagcctagat tggcttccga gtagcccatt ttctcgtata
     4021 caataaacag attggctaac agatagtcag ctaattcctc agattcttta ggtaagaccc
     4081 gcaacaaatc atatcccgcc tgcttcttcg tcatcggctg agcggtgatg aagccaacga
     4141 ctttttgatc tttacggaca ataccgacac cgctagataa tacatatgat ttatcaaatc
     4201 gtcccacaga gaagtatttt tctcgtgcac cttgaagcca tttttgtgaa acttcattta
     4261 cagcttgatg taattcttcc ggcagttgat cgtaccaagt aaaatggtat ccttctttca
     4321 tcaaacgttg atattcaagt tgctgactca gccgagacaa gtcttctgtt gctagatcaa
     4381 caatccctgc ttcacctact ttcgtgaagt gaaaacccaa ttcatgaagc atcactacgt
     4441 agctttctga tatcttataa aacgcaagct gatagcctaa acaatctgct tgtttcataa
     4501 actgattcgt tgctgcccgc atcattttag gattgccaac tggatctcct aaaacaaagc
     4561 atttgtttcc tttgatctga taaccaaaaa cgacttgatc ttggtcgtct tcttggtaat
     4621 aaaaataact gtaacctgac aagcgaagct gatggcttgt agctgtgcct ccgtatgttt
     4681 ccaataaacg gtcaaaacgg tctttttcgt actcactgcc aagcttcgta gtatcttcag
     4741 ctaaatattg ataaagcgcc actaaagcta gcaatgagat gcccaaacca ataagccctg
     4801 agaaccacac atcatctgaa ggaaacaaaa taaaatgatt tcctgttatt cgaccgacta
     4861 aaaagcgtcc gctgtgatag cctgcaattg cgtaaacgat aaacaaaaag ccgaacaagc
     4921 tagcatcaaa gattatagct ccccaagaat aaacgaattt tgttcgataa aattcttttc
     4981 tggataacca cacagctaac agaatcaaga catataccaa tatcaactgc caagataacg
     5041 tacgtgtgat tgtatttaaa ataccaaaag atagtaaagc aatcgtaggc agaaatgcct
     5101 ttttgacttt tactgaaagt gctctagcaa ggccgagtaa taaaaagcca accaatagat
     5161 tcactgtctg atctaaaaaa ttgaatgaga acggcaataa tacttgaaat agccgactga
     5221 ttgcagacag attagtgatt gttgatagaa gtaccatcat gatccccgca aaatagacag
     5281 cacttacgag cacgatatgc gcaacctttt ggaaaaacag tcgcggaatg ttatctaaaa
     5341 atcgattgat tttgactcct gtatgtgtga tgaacaatag aagtcccgtc ataaaaggta
     5401 gaacataata aaacaaccga taatataata gccaaactac tgctacatct tgccgaacag
     5461 gcaattgact cattcctaag atcatcaaaa cgtcaaaagt ccccattcca cctgggacca
     5521 tcgtcagcat tcctatcaat gtagcaatca tgaacatagg atagatcgaa agcatcgaaa
     5581 tatctattct catcaacttg ccgatgatca aaaataccgc aagggctccc gtccattgtc
     5641 caaaggatgc cagtagcaga ccaagcaagc cttttggata aaactcgaag aacaatgtct
     5701 ttctctttag ataagcaaaa aagaaaatga ctggtgctaa aaaactaccg ccgaaaagcc
     5761 agatccaata gcttcgaaaa atactttctg actgaataaa aaagacatca ataaatgtaa
     5821 gaaacgacca tacagacaaa ccagtcagca taaatagtgc gacttttgat acagtggcta
     5881 aaaccatttt tctatttgtc tgctttccat aaaaactagc ccgcagactt gctcctacca
     5941 caccaccaaa accggcgaga ttgttgatcg tgtttgttac ccacgcagca agaagaaact
     6001 cttttctttc catttttggt tttccttgtt tttctaagac tctgatcgtc acccaatcat
     6061 acaacaacat cggagtgaca cccagcagtc cagccagaat catgagcagg atcgtcatgc
     6121 ggctttgttg tcccatcctt tgccaaatat cttgccaact catgccatta gcaatatttg
     6181 cgacttgatt tgccacaaag actaacacgg agccgaaaaa aatcagtttt aaaatcagac
     6241 tatgttcctt tatccattgc agcatcaagt atcgtttatt tttcacattt tcccattcct
     6301 aacttgtata cttttgtgtc ataccccaat gttatcttat ttattttaat ttttccactt
     6361 tagacctgtt ctttgcttgc gatgatagaa aaagaaagat atcctaatgc taaaggagag
     6421 gataacatga aagtaggatt tatcggcaca gggaatatgg cacaagctat tattgatggt
     6481 ttattaaaaa aaggaacagt ctcgaaagaa gaggtgttcg tttccagtgg acattttgaa
     6541 aatgcacaaa aatttgctgc tgaaactggc gtccatgcat gcaaaagtaa tcaggaagtt
     6601 gcatcacaat cagatatcgt tcttttagcc gtgaaaccaa aaatcattga aaaagtattg
     6661 gaagaactaa gagaatatag ccagaaagtc ttattcattt ctattgcttc aggaaaatca
     6721 ttaacagatt tagaaacagc gcttgaagat cctcaggcct ccatcgtacg tgtgatgccg
     6781 aatgtcaatg tcagcgtagg agcaggcgtt tctgcgattt gcaaaaatga agctgccacg
     6841 gaggaacagc ttcagcaagc tacgatgatt tttgaagcaa tcggcagcgt ctatccttta
     6901 gcagaatcag atttcagtac attcattggc cttgctggca gctcacccgc attcgtttac
     6961 atgctgatcg atgcgatggg acggactggt gtactgaatg gtttgtccaa acagacagcc
     7021 actgagattg ctgcaaaagc aattatgggt tcttgtcaaa aattcctttc aagtgataaa
     7081 aatccatggg aacttgtaga ccaagtttct tctcctggcg gtacaactgt tgcaggtgta
     7141 gttgccttag aagaagcagg attcatccca gcaatcatca aaggaatcaa tacgacgatc
     7201 gagaaagatc ttgagatgtt gaataaaaaa taatgatagc aatgtttttt cgtgccttac
     7261 aaaaaaagaa gagctgtggc ttttgccgcg gctcttcttt tgtatttttt atta
//
LOCUS       DAFGZE010000110         7173 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.114, whole genome shotgun sequence.
ACCESSION   DAFGZE010000110 DAFGZE010000000
VERSION     DAFGZE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.114"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            61..816
                     /locus_tag="LTU54_002745"
     CDS             61..816
                     /locus_tag="LTU54_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HBL8297397.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            813..1637
                     /locus_tag="LTU54_002746"
     CDS             813..1637
                     /locus_tag="LTU54_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297398.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            1938..4037
                     /locus_tag="LTU54_002747"
     CDS             1938..4037
                     /locus_tag="LTU54_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HBL8297399.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(4075..6132)
                     /locus_tag="LTU54_002748"
     CDS             complement(4075..6132)
                     /locus_tag="LTU54_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HBL8297400.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(6246..6458)
                     /locus_tag="LTU54_002749"
     CDS             complement(6246..6458)
                     /locus_tag="LTU54_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297401.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            6645..7112
                     /locus_tag="LTU54_002750"
     CDS             6645..7112
                     /locus_tag="LTU54_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297402.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
BASE COUNT         2428 a         1132 c         1351 g         2262 t
ORIGIN      
        1 ggagcagggg gactttctgc agttgtttac tttaagaaga aagcctagaa ggatcagcta
       61 atgattattc gtaagccccc aaaaagaaga gtcatgcaca aactgatgat atttttttgg
      121 ttgctgtcgg cagtagcagt ctatttaatt ccagttgtgg aaacaaatgt agcgaaattt
      181 caacaacagc aaagaatttt agccattgaa aatcttgaaa aaacagattc ttctttggat
      241 gttgcgaaaa tagagttagg cgatcctgtt ggtgttctta caattccaag tatttcttta
      301 aaactaccaa tttatgacgg aacttccgac aaaattttag agaatggtgt agggattacg
      361 gaaggaactg gcgatattac aggaggaaac ggtaaaaatc ctttaatcgc ggggcacagt
      421 ggactttata aagataatct tttcgatgat ttgccttcag taaaaaaagg gcaaaaattc
      481 tttattaagg taaatgaaga acaacatgcc tatcaaattt atcgaattga agaagttaaa
      541 gcagaagaat tacaaaaaaa ttatttagat tatcttatgc ctgaaaaaga ccaagatagg
      601 atcacgttaa tgacatgcac accaaaaggt gtcaactcac accgcttttt agtgtatggt
      661 gaacgagttt ccttcagtaa agaagattta aaactacaga aaaaagcaaa aaataggaaa
      721 tcaatttcat tggattggcg attagcactg ctattcgctt ttttttgcgt tcctattatt
      781 tttatttata aaaaaataag gaggagatcg gaatgaataa gagaaaatgg ctgtttgttt
      841 gtgtagcagc tctcttttct actgtagctt attcgcaact gtctttcgct gacagcagtg
      901 atagtagtac cactcaaaca atagataatt caattactgc accaagcgaa acggacagtt
      961 cggagaatac tacagaagaa acggcagatt caaatataag ctttactgaa tcaacgaatc
     1021 caacgtcaga aagttcttca gagacttcag aaaagccgca agacgtagaa gatacgcaag
     1081 tgatcaagca agccattcat attcaaaaaa ttatttcttc gaatcaaatc aacaatcaag
     1141 gcagtcaagt agcccagcaa gaaggtatca atggcgcgaa gtttattgtc tacgatatca
     1201 cgaatatctt agaagaaatg tcacaagatg ataatgagaa aaatcaatct gtagaaataa
     1261 ttgagtcaaa attgaaagat cgagcaaaaa aactttcata cgaccagttg aaaaaagtgg
     1321 ctgaaggaga aaccaaaacg attgatgatc aggaaggcgt gattgaattt tccattgaag
     1381 tacctgcaaa caaaaaacaa gcatactata tcgttaatga atcaagccct gaaaacattt
     1441 caaacagtga agatattgtt ttattgacac ctgtttcaaa tgaaaaaggt gaatttttga
     1501 cggatgtttg gttataccct aaatcagaga aatcagagcc gaaagaagaa attaaaaaag
     1561 ttgtgtcaac tggtgtaaag aagaactttt ttgaaaattg ttgggaattt tttgctcaat
     1621 tattttttga taattaggat gaaatgatga aaataataag aatattagtt tttaatctat
     1681 cctttttagc cgcaacaata ttgtgttgcg gtttaatttt ttctcaatta tcaatttatc
     1741 caagatatca acgattgttc attcattttc taaacattca atgtgcttcc atgccattag
     1801 aggtgattaa aaagctcttg atcaatccaa tttttatggt tagtttggtt cttcttacag
     1861 gattggtaac gtgggtatta ggaaaaaaag aatgaaaagc ttacgatcgt ttaaaaaaat
     1921 attagatgga gggcattatg gaaaataaaa ttcattgcac tacgggaaga gtaattactt
     1981 tagtgttgtt tttgttcctt cttctttttg cgaggggaac tgcagtccag gccttagaaa
     2041 aagttgatcc gagtcaaatc aaagaaaata ataatctggt gctttatcat gatagtgatt
     2101 cattgagaaa tatcgaagta actgggatca atggatttaa gatactagat gagtattcaa
     2161 ctaaaaaaca aaaagtactt gcttggccaa aagtcaaaga tttgaaaagt acgtcaaaaa
     2221 tcgttgccca ttatcaaaaa gtaggtactt atctaggaaa agaaattgat tgtgacgtga
     2281 ctttttctaa ttttgtagcg cacgctgggg ttaaccaaga cggattagct aaaacaatta
     2341 atggcaaaaa gtattataat tatttgctct ttgataaaaa tatttatcga ggatttttca
     2401 tgcagtcaat caatgtcatg aatgcaaaac tagaatttaa gtatgcagga acaaaaacaa
     2461 aagtagcttt gagtgaacca cagccagaaa ttcaatcaaa aatgagatcg atgagagcag
     2521 ctggtattga tttaaacgat acatttctat ccatttacag tttaaatgga cagtatcagc
     2581 aacgtgtgtc ttggtataat gacaataatg aatctgtcgg tgaacgtaat attgatatga
     2641 gagaatttgt tgggtatgaa aaaatgggta gcttacctta ttttgtcaca acagatacag
     2701 catgtgcaga atacaaagct cctgcgttat caacaaacaa tttaacttca aaagtagtgg
     2761 gaggacgtgc agaaaaggct tatagctcga atgatcattt caccgatgtt gtaggagctg
     2821 atacttatca cagaagtggt gtaacgtata cgcttcaagg cgcttcccca acattcatga
     2881 ttggcgcaaa tacgaatagt atgatgttta gctttgatac tgcattgcta tggacaccac
     2941 aaccatcgaa gcctacaaaa gaagtgttta acaaagctaa tactgaagag gcagcacaca
     3001 atattgacaa aaaagtgatt ccacaaggat cagatgttta ctatcatatt caccaaaagt
     3061 ttgatgcatt aacagtcaac acaatgaaca aatacaaatc atttaaaatc actgatacct
     3121 ttgacagcaa aaattttgat atggtatcgg atgggaaaaa ctatgatggc gcattgtggt
     3181 atgttgaagc gaatggtact tggaaaaaag ctgatacagg aggaaagtta actgtatcag
     3241 gtaatacagt cacttatact gcttctgaca gctttttagc taacgctgca aattacggta
     3301 gggattatat tttgcagctc catatgagag caaaaaatga tgcaaaagga attgcaccaa
     3361 atgtggctac tgtaacgttt aacaatgatc cgaacaaagg aacacaaaaa acaaatacag
     3421 tagaaaacta tttctctctt aaaccaaata aaaaagtgga acaagatggg aaagatgttg
     3481 atggtcgaaa tgatggtaaa aaaggaacgc ctacagctcc tttaaatgca ggatctgaag
     3541 tacaatatct agtcacacaa aaatggcata caaaaggtgt agacattgcc tcagatcatt
     3601 acaagcaatt tagtattcaa gatccagtgg aagatcgttt gacgtataaa gaaggttctg
     3661 cgcgagtgat agatatgagt acaggaaaag atattacttc agaaggtaca ctaacctatg
     3721 atagcaattc aagaacgctg aagtgggaag cttccgctga tttcttaagt aaaaatcttt
     3781 tagatggacg agaaattcag ctgatattta cagctaaaac tcctttgaat gaagaattgg
     3841 atatcgacaa ccaagcaaaa gtttcagtag aaagtattga gaaagcaact aatgtagtaa
     3901 cgattggggt aaaacctaac ttacctcaga tcattgtgcc aaaaactgga tcgaatcatt
     3961 tagtgctaac tgttattgga agtatattat ttcttacttt agcatcattg ggctatgtag
     4021 ttttaaaact caaataaata taaaaacaag gctgagaatc agccttgttt tcttttattt
     4081 gaagattgga ttcgcactaa taaatacatt atctacgcca attctaccag cagagtcaac
     4141 tacaaaatta tgacctaatc cgagaatact ttggttatta taaagtaaat ttgcattatg
     4201 ggcagggcta tttttccatc ctaccatggc atcgatagca ccatcgtctg ataaaaggtc
     4261 atcaatatac agcccagttt gacttatgtt ttctccctta gcaccgtagc agcctaagct
     4321 ttccaataaa tcttggaaag taccaccacc agggcgatag tgactgaagc tgacttgtaa
     4381 ttctttagct cgaatatctg cagcctgttg aacaatatca ttgaattgca ttggggcaac
     4441 accattttgt tggcgataat catttaacca agcaagttct ttttgacgca taaagttacg
     4501 taattcgtct aatgaaatct ttttatattt gaatgtgatt gttttatttt gatcgacgtt
     4561 attaatggat cgttggccgt ttttatccac ggtaacggta ggatcgtcat ttctgcacaa
     4621 gaaataattt tgatcagtca tgtttttcca aggagtagca aagctatccc cttcgtatac
     4681 tgtttttgat tcactagaga gaacagcgtt ggtatcagca tttacatgat taacagtcac
     4741 attaaattta cgagtgtagt tgaaaatata attttgactt tcagtcacat tgtttaaggt
     4801 tactgaattt gttggttcta cccgatcgtc taaaacatat ccttgaatag aaggagcagt
     4861 taaagtgaca ctgtcaccgt gagtgacatt gactatctgt tctttcaaaa tattaactcg
     4921 tccttcgttt ttgtagttgt tattgcacaa caaattgatt tgatgcactt gattataatt
     4981 gaatgtgatt gttgtattac ctgtgattgt tttgctttga gtatcattac caatcacttt
     5041 gtagctaaaa ttatctttag cgccttcgct atatttaaat gcttttgcct tagcagtgta
     5101 agtagaacca gaagttactt ttgcttgatc tgtagcaatc acttgacctg tttgattgtc
     5161 aatatggtta atagtaaccg tatattcttt tggtgtttca ggtttgtgtt gcaacccttt
     5221 aaatgcagca tcaagactat ttttagcatt gtttacttgt gtttgagaaa ccttagaatc
     5281 gtctagtact tttttagcat tggataaagc agtctgaaaa ttattccaag attcttctgt
     5341 aaaatctcct ttagcagttg attttactcg gttgtataaa gcttgtaatt cagctttatt
     5401 cacagtagga actggttttt tctttaatcc tttgtaagca gaatctaatt tatttttggc
     5461 actatccact tgcttttgag tcgcttttga gtcatttaac actttcttag cattgtttaa
     5521 agcggtttgg aatgcgttcc aggtattgtc ggtatagtca ccttttttcg ttcccttaac
     5581 gctcgtataa agagcttcta gagccgattt attcactttg ggtttagagg tgtcagcgcc
     5641 ataccaaccg ataccttcgt atttccagcc atagtctttg gttaaccatt gtacttcttt
     5701 ccaacttgca gtgtagtgat gtgagccaga ttttacacca ggaaggaaaa ggcggtagat
     5761 cggtttatta tttttttcgg cagaacgcca ggcaggccct tcataagtcc agccgtaatt
     5821 tttagtcaac catttcactt catcccaact agctgtgtaa tggtgatcac gaagaccttt
     5881 attgtacaaa cgatagacaa gatggccacc gctagtcggt gcttcccagg cggggccttc
     5941 ataatttccc cattttatct ttactagatg gtcacgttct tttttgctag aggtgtaaaa
     6001 atgttcatta ttatttggat tgtaaaggcg atacatgacg ttagttgaag cagcgtcaac
     6061 agatccagca agactaagtg aggcagcaaa tgttccaata gctaaaacag tttttggatt
     6121 aaataatttc attttttcaa ctcctaaatt taattaaaaa tgtttacatg attattatat
     6181 atcggctaac cgatatagtc aataaaaaat aagaaaagcc ttatataatt ttggcttttc
     6241 ttattttatt tgttagttga agtattaata tcaacgattt cttcagccaa tttatttaat
     6301 ttcatagcat ttatgaatct catgttgtct atactagagc gtttgttggc aaggtcactt
     6361 actgtagtta agggtacgcc agattctatg gatatagaac gaactgattt accactatca
     6421 aacagcaacc attcgatttt ctttttatca gcgtacattt caatgctcct tcttgttttt
     6481 tctttattat agctcttttt tgtaatgaat cacaatatca agagaaaaat tttctataga
     6541 aaggtggatt tttatgagta ttcgttctcc gtgtatccaa atgaggtttg ttgctacttt
     6601 cgttatttga tatactaaaa ataaacaacg aaagaaggaa taaaatgaag aaaaagcaac
     6661 tatggtttgt ctctatgttt gcaatatttt ttattatttt aagtggatgt tcaagtaata
     6721 agaaatcgga agctaaagaa gataatagtt tattgcaacc aggaactgtt tggaaagaag
     6781 aagtaggtgg tcttattcat aatttaaaaa ttatagatga tactacctgg gaatattctg
     6841 aaagtgtatg gcatccagat cctgtacaaa taacagttaa gaggcaaaaa gactatgaag
     6901 gtttagaaag atacaaaata gtagattctg caggcattag ggaatttata aacaaacctg
     6961 attcattatt tattgttgtt ccctatgaga aggatggcgt aaaaaaaata atttttcttg
     7021 gatctagcaa ggatgagaag cgaacgaaag agaaattaat tcatgatgga tcagagttaa
     7081 ataaatacaa attacaaaag actagtgagt gattgctagt ctttttattt atcctgaagg
     7141 aggaatcaca gtggcaaaag ttaaaacacc att
//
LOCUS       DAFGZE010000111         7096 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.115, whole genome shotgun sequence.
ACCESSION   DAFGZE010000111 DAFGZE010000000
VERSION     DAFGZE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.115"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            1..405
                     /locus_tag="LTU54_002751"
                     /pseudo
     CDS             1..405
                     /locus_tag="LTU54_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
     gene            421..570
                     /locus_tag="LTU54_002752"
     CDS             421..570
                     /locus_tag="LTU54_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297403.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            651..1247
                     /locus_tag="LTU54_002753"
     CDS             651..1247
                     /locus_tag="LTU54_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL8297404.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            1260..2159
                     /locus_tag="LTU54_002754"
     CDS             1260..2159
                     /locus_tag="LTU54_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL8297405.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            2162..2293
                     /gene="ssb"
                     /locus_tag="LTU54_002755"
     CDS             2162..2293
                     /gene="ssb"
                     /locus_tag="LTU54_002755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL8297406.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            2315..2647
                     /gene="ssb"
                     /locus_tag="LTU54_002756"
     CDS             2315..2647
                     /gene="ssb"
                     /locus_tag="LTU54_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL8297407.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2669..2926
                     /locus_tag="LTU54_002757"
     CDS             2669..2926
                     /locus_tag="LTU54_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297408.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(3085..3207)
                     /locus_tag="LTU54_002758"
     CDS             complement(3085..3207)
                     /locus_tag="LTU54_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL8297409.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            3397..3621
                     /locus_tag="LTU54_002759"
     CDS             3397..3621
                     /locus_tag="LTU54_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297410.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            4069..4275
                     /locus_tag="LTU54_002760"
     CDS             4069..4275
                     /locus_tag="LTU54_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL8297411.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            4275..4526
                     /locus_tag="LTU54_002761"
     CDS             4275..4526
                     /locus_tag="LTU54_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297412.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4542..4979
                     /locus_tag="LTU54_002762"
     CDS             4542..4979
                     /locus_tag="LTU54_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297413.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4972..5667
                     /locus_tag="LTU54_002763"
     CDS             4972..5667
                     /locus_tag="LTU54_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297414.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            6048..6227
                     /locus_tag="LTU54_002764"
                     /pseudo
     CDS             6048..6227
                     /locus_tag="LTU54_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6289..6473
                     /locus_tag="LTU54_002765"
                     /pseudo
     CDS             6289..6473
                     /locus_tag="LTU54_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6507..6938
                     /locus_tag="LTU54_002766"
     CDS             6507..6938
                     /locus_tag="LTU54_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBL8297415.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2543 a         1191 c         1447 g         1915 t
ORIGIN      
        1 aaatgaccgc caaatgggaa agctacttgt taaagatcgg tgaacgaaaa ggaacacaaa
       61 ccacttttct agccaatatc caaaaatttg tctctcactt attggaagtc gtaccagggc
      121 aaatccaatc cactgatttt ggatcgacgt tacaagaagt gaaagcagcc agtgaaaagc
      181 aagaagccgc gcgtcattta ggaatttgtc caaaatgcca ggaacaagaa gtcttactct
      241 atcaaaaggt ggcagcttgt acgtctgaag cctgtgattt taaaaggtca gaagggttca
      301 tttgaagcaa cgattgtttt aaaagaagac ttcactacag gctttgaatt ttctgaaaag
      361 aaaaaaacga acttcaagaa aagaacacgc cgcactgcaa aataatggag ggttaatctt
      421 atgcgtaata ccacacacaa acaacaagaa atcgaaaatc aaaaagccaa aattcgttca
      481 gaacaggaaa aaatgcgtgc cagcaaacga agactgcagc aattgaagaa ccagcagagg
      541 attcaaaaag aaatcgaatt agatcgataa aatcttccta actattgtat acgtataaac
      601 ttatgttata ctgtttttat aagtttatac gtatacaaag gagggagaaa atgacaaaag
      661 caatggtata caagctgcta gaagaattta atggaacttt ggctttgaag gatgccaaaa
      721 aagcgggcat ttctcctgta acgatcaaac gtatggtaga tcgaggggag ctggatcgag
      781 agtatccagg ttttttcacc ttacctggtc aatttcctga tgaattgttt atggcgcaaa
      841 aaaaatatga acgcggcatt atttcacata ttaccgcgtt agatttgtat gatttaacag
      901 atatgatccc tagacagatc gatctgactg taccttacgg ttatcacgtt tctgaaaaag
      961 gattgaagga attcgcagtt gagctacact atacaaaagc ggaatggtat gaattaggta
     1021 agattgaaat aaaaagtcgc tatgggaatc ctattatagc ttatgatcca gaaagaactt
     1081 tatgtgacat ttggaatccc tggtataccg ttgaggatga aatcaaagta aaagcaatca
     1141 aaaactatat ggagtcggac agaaaaaatc ttagaaaatt gaatgagtat cgacgaattt
     1201 taccaacaga caaaacgatg cgttcttata ttatggcttt aaattaaagg agaagataca
     1261 tgacacctac gcagttaaaa gcaaaagtga agaatatttc tagagaaaaa gaagttgatc
     1321 cgcagcttgt tttaagacat tttatgatgg aaaaattctt ggaaaaaatc tcagagtctc
     1381 cttatcgtga aaattttgtg ttaaaaggcg gcttcttgat tggctcaaag tatggcattg
     1441 aaaatcgcac aaccaaagat attgatacga ctttacggga aatgaaagta accaaagaaa
     1501 cattaacaac ggttctaaac gaaatttttt caacgccgac gaaagaaggc attcaatttg
     1561 agattcaagg aatgaaagaa acgagagaag ccgattacta tccaggattt agcttacgtg
     1621 tgctggcaca tttggaaaac atgcggccag attttaaggt agatgtgact acaggtgact
     1681 cgatctatcc tgcaacgatc actcacagtc ataaattgat gtttgaggat cgaacgattc
     1741 ctttggaatc ctatcctaca gaacaaatta ttgctgaaaa attaagtgca acctttgatt
     1801 ttttaacaga taattcgaga ggaaaagatt tttatgatct ctatactatt cctaaaatgg
     1861 agaagatcga tgagaagacc ttgaaagata gtgtgagaaa tacatttaca cgtcgaggaa
     1921 aaactgatcc gttaaagata tactatgaaa aggatatgga tgtactgaaa aatagtcacg
     1981 agctaaaaga tacctggaag aaatatcaaa aaagcaatcc ttatgctgcg tcgatcacgt
     2041 acgatgagac aatggattcg attagtaatt tgatggaaaa agtgattcac gtcgaagaaa
     2101 aagagcgaaa actagcttat caaagacacc aacagatgaa tagagaaatg gagagataaa
     2161 gatgattaat aatgtgacat tagtaggacg cttaaccaaa gatcctgatt tacgttatac
     2221 cgcaagtggt actgcagtag cgacttttac cctagccgtc aaccgaaatt tcacgaatca
     2281 aaacggcaat tgagaagccg attttattaa ctgcgtgatt tggcgcaaac cagcagaaac
     2341 aatggcgaca ttagctaaaa aaggtagctt gattggtgta gttgggcgga ttcaaacgcg
     2401 gacttacgac aaccagcaag gacaacgtgt atatgtgact gaagttgtag cagataactt
     2461 tcaactatta gaaagtaaag cagcgacaga gagccgtgcg cacgctgatc aatcgtccac
     2521 cagtccaagc accgccacct ttgaacaacg gaatacagcg acgcctaaca acaatggatt
     2581 gaacgcttca cagaatccat ttggtggaca atcaatcgat attagcgatg atgatctacc
     2641 gttttagttt gagaaagaag gagtaagcat ggcaaaaaaa attagtctga cgatggatct
     2701 attgataccc gtaattgcat ttatagacgg gatttctttg ggcgtcactg aaaatccttt
     2761 ggtaaataga attgcgaaag gatcactatt tcttgtattc gtgtatcttg tctgttctta
     2821 tcttttaaaa agagcaggaa aggataattc cttttggcga ttgactaaag tcacagtagc
     2881 agcgacagct gttatactta cggctattct accgctttat atgtagagtt aacgggaaat
     2941 cgttgaactt cttgcgaaat attgctataa tgaacatgaa aaagaggagt tgcgctaaca
     3001 acccctcatt aacagcaccg attaaagaac ggtggcagaa tattagttat ttggaaataa
     3061 ccgctgacct gctaaagtag acggttattt ctttttgtca tttttaagca tatttacaat
     3121 caaactaatc aaagcaatgg taaacatacc aaaaccaaga atggtttgaa ttgtctcata
     3181 tgcggacaaa aggcttactc ctttcataga ttttagcact tacattcata agcaccacca
     3241 cctttccaga agtcgccacc gtcttcaaac tttactgtct tgactattat agcatatcta
     3301 aaatcgaata aaaattctca attttagctg tttttgctat aattttagcg aggtgatcgt
     3361 gatggaacaa tatattggtt tactcgataa attagggtga tcaagacaca gccattgctt
     3421 gtgcgattta cattacgcac gagtcatgaa ccaatcaatt gtgtagtggc caatatagaa
     3481 atagcggata aattgctgat tttggaagaa ggcaagtata ctatttcagt gacaggtcac
     3541 ttcaataaac gaaatcaact agtcattgaa tcaatgcaaa ttagaaaccc tgatcatttt
     3601 acgagattaa tgggcatata aataaaagaa gagctgcagt cctaaatgag gattgcagct
     3661 ctttttgttt tacatagaaa caagaaaaat gaaaaggcct ttttaggaag gcgcaagcgc
     3721 cttctcaatt gaaaaacctc tctctcactc caaccgccac cgccgttcga gaagaaagga
     3781 agtgaacgtt gcacgttcac taagataagg ggtcttgcaa agcaagacaa aatagaaaaa
     3841 acttcctctc accaaaaggt tcgttcctgt tcgctatctc tttattgatt tgccagaccg
     3901 gcaacaagtt gccttttatc accaaaaagc gacaaggaca agggtaaatg gcacgctgca
     3961 cgcccttgtc cttgtcgctt ttagttgatt cattctggcc aacaaatcaa taaagaaaga
     4021 actaacgaaa ggaagtaaaa cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa
     4081 tttgattatt ggggtgttcc cttcagcgaa ttattcccaa actatcatgc accatatact
     4141 gtcgaatgcg attgtggaga aagagcgaaa tgtatcaaaa gctattgttt gtatcaatgt
     4201 ccaacatgcg ggaaaaaata taccttgtct tatggcgatt atattttgat tgatgagaaa
     4261 ggaaagaaac gctgatgaaa acacaacaca aattattgac tgtaaaagaa gtcgttcaaa
     4321 ttttggtgga caaagaagtc atttctgaaa aagtagcaga agctttactt attccgcggt
     4381 actggacgat agaaacccct ttagtagagg atgcaccgac tatgctattt gaagacctag
     4441 tgaagattgc aggatatgaa ctgcattatc aagaaaacat ggaaacttat gaaaacgaat
     4501 attggttaac caaattagag aactaaaaag gagaaaaaaa tatggctcaa aattactatg
     4561 acgaatttgt caaattacct ttggataaaa tggcacaaaa aatggaggat atgacattcc
     4621 tatacaatga gacacgtgtt ccaaaaaaac attataagga aagattgtcg gtagctgtgg
     4681 aagaaatgat tgaatcgggt gtcgaaatga atctcattgc gacctactat cgaacgcttg
     4741 aagaactgaa aaagcaaaat ggacaatggt ttttccaagc cttactatgc ttggaagcag
     4801 gcgtcaagcc aaacactatc aaaccttctg aataccaagc tttagaacta acgtatggga
     4861 aatttgtcga aacgaaaaaa gccaagactg tttcaagtga atggttggac tacttagaaa
     4921 atgtcaacaa gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgaataag
     4981 gtgatttatc aactaacaac ggaagatgtg tcacactttt taacaaacaa agaacatttg
     5041 aatgaagtaa tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt
     5101 attcaaagag tcgaagaaca attgtatttg gaaagtgaaa cgagtcaaac gaaggttaca
     5161 gtcagcaata attatttaga cgtgtataaa cgtcgtcctg acacattaaa aaaaggagcg
     5221 gagcttctac gactagaaac agaaaaatat atcatgaatg tttacctaga aaggagtgaa
     5281 ctggtcgaaa actttgtcag ctacacgatt tgcaggaaac actattactc agaatcagaa
     5341 tattattcaa tttttgagag catggaatta atggaagagc taaacagcaa agatgcgacg
     5401 gtagacgatt attaccttgt atttactaaa tttttgacgt gtgggacgga atctatcgtg
     5461 ttaatgggaa tggattatga actcgctgta cccgaagtat ttttgaggga atatgtagaa
     5521 gaagtagata acccaggcga cgactggtac acgactttaa ctgatttttt agatacctac
     5581 acttacgacg atacagaaga aatccaaaat gaacttgacc gtaaacgaat gccatatcaa
     5641 cgatttgaaa ggaaaaaagg tcaataacca tttataccga aaagtgagaa tgtgtgaaat
     5701 aaaaacaaaa aaccttgaaa ttaagggaat ggttcttatt tcgcaccaat cgctccaacc
     5761 gcccccgccg cttaggtggt gcgaaataag cgagcaaagc ccgaaaacct aagagaaaga
     5821 aggagattta tatgaacaaa attattccct tattattagt cctatttgtt ggagaaatac
     5881 ttatgcgact tagtgcagct gtacatttaa acctcgcagt atttttggta atactaatgt
     5941 tttgtttgat cttttggcga tttgtattag ccctcgtaag tctactattc aagatgattg
     6001 cagccatatt tttcatagga ttatttcgat gaaaggagag agtaccaatg ccaattgcaa
     6061 taggaaataa acggttaccc gtgacgttgg atgaaaagag acaaaaagaa ttgcagtaac
     6121 caaagcagaa gtacggcaaa agtgaatcca ggattatgtg tattgcgtta gatttattga
     6181 ttgcgcaaga aaaagcagga tttgacatac cagcactcaa aaagtgatcg cactttgcgc
     6241 acgtgttaat acgtgttata attattttgt aaggaggtat agtgcacaat gccacttacg
     6301 ggaaaagaaa tgcttaagtt attgaaaaaa atggctgggt tgagcgaagg caagagggat
     6361 ttcatcatca tctttataag gatggtgtta gaatcactgt tcctgttcat gccaatcaag
     6421 atcttggccg aggacttgag cgaaaaattt taaaggatgc gggattgaaa taatctcgca
     6481 ttcgattaaa gaaaggattg actgaaatgt taagtgagcc tttagcaaaa acctatcctg
     6541 ctatttttag cccagaagaa ggtggtgggt actttattga atttcctgat gttcaaggtg
     6601 cttacacagg aattaacgaa gacgatatat cttatggaat tgctatggca caagaggtgt
     6661 taggtatggt tttagcagat tatattgagc atgaagatct acttccagaa ccaacaccaa
     6721 tcaataagat ttctgttgaa gatgattctt ttactacttt aattcgtgta gatgttgcca
     6781 aatatttgaa agacacagaa cttgtcaaaa aaaccttaac aattcctcaa tgggcggata
     6841 agctaggcaa aagggctggc ataaattttt cagtccttct aacagaatca attgcagata
     6901 aagcagataa cattcttcgt tctggaagaa ataattagaa ctcaaaaggg tcagaaactt
     6961 aaaaatcttt ggcttctttt tttactacaa agatggcttc tttaaattat ttgcctgatt
     7021 gatttaaaaa aattgcgcaa agactacgag ctaattaagt tgctcgtggt ctttttttat
     7081 gaaaactttt tagatc
//
LOCUS       DAFGZE010000112         6967 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.116, whole genome shotgun sequence.
ACCESSION   DAFGZE010000112 DAFGZE010000000
VERSION     DAFGZE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.116"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..203)
                     /locus_tag="LTU54_002767"
     CDS             complement(<1..203)
                     /locus_tag="LTU54_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL8297416.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(640..900)
                     /locus_tag="LTU54_002768"
     CDS             complement(640..900)
                     /locus_tag="LTU54_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297417.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            975..1178
                     /locus_tag="LTU54_002769"
     CDS             975..1178
                     /locus_tag="LTU54_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HBL8297418.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="LTU54_002770"
     CDS             complement(1319..1804)
                     /gene="ssb"
                     /locus_tag="LTU54_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL8297419.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(1807..2706)
                     /locus_tag="LTU54_002771"
     CDS             complement(1807..2706)
                     /locus_tag="LTU54_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL8297420.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(2719..3312)
                     /locus_tag="LTU54_002772"
     CDS             complement(2719..3312)
                     /locus_tag="LTU54_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL8297421.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(3388..3537)
                     /locus_tag="LTU54_002773"
     CDS             complement(3388..3537)
                     /locus_tag="LTU54_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297422.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(3553..5757)
                     /locus_tag="LTU54_002774"
     CDS             complement(3553..5757)
                     /locus_tag="LTU54_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HBL8297423.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(5768..6790)
                     /locus_tag="LTU54_002775"
     CDS             complement(5768..6790)
                     /locus_tag="LTU54_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL8297424.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         1762 a         1425 c         1213 g         2567 t
ORIGIN      
        1 cgtttcttta ctttctcatc aatcaaaaca taatcgccat aagacaaggt atattttttc
       61 ccgcatgttg gacattgata caaacgatag cttttgatac atttcgctct ttctccacaa
      121 tcgcattcga cagtatgtgg tgcatgatag tttgggaata attcgctgaa gggaacaccc
      181 caataatcaa atttctttcg catgattgat tctgcctcct cttaggaaag gttttatttc
      241 ctttcgttag ttctttcttt attgatttgt tggccagaat gaatcaacga aaagcgacaa
      301 ggacaagggc gcgcagcgtg ccatttaccc ttgtccttgt cgctttttgg tgataaaagg
      361 caacttgttg ccggtctggc aaatcaataa agagatagcg aacaggaacg aaccttttgg
      421 tgagaggaag ttttttctat tttgtcttgc tttgcaagac accatatctt agtgaacgtg
      481 caacgttcac ttcctttctt ctcgaacggc ggtggcggtt ggagtgagag aaaggttttt
      541 caattgagaa ggcacttgtg tcttcctaaa aaggtctttt cctgtttcta tgtaaaacaa
      601 aaagagctgc aatcctcatt taggactgca gctcttcttt tatatgccca tcgatcgcgt
      661 aaaatgatcg gggtttctaa tttgcatcga ttcaatgact aattgattcc gtttattgaa
      721 atgacctgtg acggcaatat tgtattttcc atcatccata attaacaatt tatcgataat
      781 ttcaatatct gcaactacac aattaatcga ttcatgaatt gtttgtaaag taaagcgaac
      841 caataatggt tgtgttttta ttactcggat tttatcaagc aaaccaacat atttgttcat
      901 cacgatcacc tcgctaaaat tatagcaaaa acagctaaaa ttgagaattt ttattcgatt
      961 ttagatatgc tataatgatc aagacagtaa agtttgtgac ggtggctatc cgattgaaag
     1021 tgaggtggtg cgtatgtgcg tacttccaaa atcttacaga aaggagaagc cttttgcctg
     1081 ttgaaacggc gctaggatta atgattagtt tcggttcttt gatcgcaaca atcatttttg
     1141 gaatcttagc agttgtaaac aacaatcaaa aaaaataacc gtcagcttta gccggtcgac
     1201 gattattttt caaaaataat attaagctgg tcaccgtctt aaacggtgct gttaggggag
     1261 ttgttagcgc aactcctctt ttcgtatcca ttataacaac tttattgatc ttttacaatc
     1321 agaatggtaa atcatcatca ctaatatcga ttgattgtcc gccaaatgga ttttgtgaag
     1381 cgttcaatcc attgttgtta ggcgtagcta catcccgttg ttcaaaggtg gtagggcttg
     1441 gactgctgga cgattgatca gcgtgcgcac ggctttctgt cgctgcttta ctttctaata
     1501 gttggaaatt atcggccaca acttctgtca catacacacg ttgtccttgt tggttatcgt
     1561 aggttcgggt ttgaatccgt ccaacaacgc cgatcaaact gcccttttta gctaatgtcg
     1621 ccatcgtttc tgctggtttg cgccaaatca cacagttaat aaaatcagct tctcgattgc
     1681 cgttttgatt cgtgaaattt cggttgacag ctagggtaaa agtcgctact gcagtaccac
     1741 tcgcggtata acgtaaatct ggatctttgg ttaagcgtcc tactaaagtt acattattga
     1801 tcatctttat ctctccattt cttgattcat ttgttgtgat cttttaaact gccgttttct
     1861 ttcttgattt tcaattttga tcacactttt cattaggtga tcaatttcgt tgactgtatc
     1921 tgtaaatgaa atggaattcg caaacgtgtt aactgagcga tatttttccc actgcttttc
     1981 caaatagtca tttcctctga tcttatccat ttctctttca taataaaagg acaacggttt
     2041 tgtatcccca cgttttgtaa atgtatttcg aacacttttg tataactctt tcgtatcgat
     2101 attttctagt ttcggaatgg taaataagtc gtagtaatct tttgctctcg aattatctgt
     2161 gccaaagctg aacgtagcac ttagcttttc tgcaataatt tgttctgttg gataagctgg
     2221 aatctccact tttttatctt caaacattaa ttggtgatag tgtgtatcta cttcaggata
     2281 aatagaatct cctgttgtca cgtccatttt aaacggtaca cgtactttct ctaaattagc
     2341 caccaattta atttgaaatc caggataaaa atcggcttct cttgtttcct tcattccttg
     2401 aatagagaaa acaatgcctt cttttgtggg ttttctagtt aaatcattaa aaatgctctt
     2461 caatttttct tcagttaatt tgctatttct aaatgtcgta tcgatatcaa ttgttgtcct
     2521 tctatcgatt ccatattttg atcctaataa aaagccgcct ttcaaaatga agtcattttt
     2581 gtactttgat tcagaaattt gttcaattag acgatccatc ataaaatgcc gttgcactaa
     2641 ctgtgcattt aatcctgtac tctttgctaa atttcttgtt tttgctgcaa attgtgtcgg
     2701 tgtcatcttt ttcctccatc aatataagct ttgcatgtgg tatttcattt ctttttctac
     2761 atgtagtttt gtcatgtaat ctcttaattt acttggatct ctcaatgggt cttccatgta
     2821 ctcttttaat gcttctaatt tcatttcata ttcaaattca tatctcggat tccaaatatc
     2881 acaaagtgta cgctctttat catacatagt aataggattt ccttgaaagg aagttccctc
     2941 tacttttcct agttcataaa cttctttctt cacgaaatga aattgtaact gctgctcttt
     3001 tgtaattgaa cgatcaacgt tatagccatg gggaaaagtt aagtgaatga atttcggcat
     3061 attcgtagaa agctggtaaa tatctaaagt cgtttcatga gagaagattc ccctggaaaa
     3121 tcgttgctga agccaaaata aatcatctat tccatgacca tcctctacat aataaccacg
     3181 atcaactttg atcaattttc cttgattcac caatctttgt acagtggttg ctgcgattcc
     3241 aaatttcctg gcttccttca tagataaatt tccatcgcga atagccaaca attctttcag
     3301 tttatcttgc atttacttcc cctcccttcg ttacaaatac actacatcaa atatatcata
     3361 gtagtttatt tgtaacaaga ttaacgtcta tcgatctaat tcgatttctt tttgaatgcg
     3421 ctgctggttc tttaattgtt gcagtcttct tttatttgca cgtaatttct cttgttctaa
     3481 acgaattttt gctttttgat tttcgatttc ttgttgtttg tgtgttgtat ttcgcatagg
     3541 ataacccctt cattattttg tagtgcggcg tgttcttttc ttatagttcg tttttttctt
     3601 ttcagaaaat tcaaagcctg ttgtaaagtc ttcttttaaa acgatcgttg cttcaaacga
     3661 acctttctga ccttttagtc ctctgactga ttggcttgtt cggccgtttt gaatgatctc
     3721 tttcagttgt gtggctgtga gtttcttttt ggcaatcgtt gtccataatt taaaatcaca
     3781 ggcttcagac gtacatgccg ccacattttg ataaagcaag acttcttgtt cccgacattt
     3841 tggacaaaca cctaaatgac gcgttgcttc ctgtttttca ctggctgctt tcacctcttg
     3901 taacgttgat ccaaaatctg ttgactggat ttgtcccggt acgacttcca acaagtgaga
     3961 gacaaatttt tggatattag ctaagaaagt agcttgtgtt cctttctgct caccaatctt
     4021 taataagtaa ctttcccatt tggcggtcat ttcggcactt gttagcagcg gctctttttc
     4081 cacggcttgg caaagtaaaa tccctttatt tgtcaccaca agcttatttt tttctacctt
     4141 gatatattcc ttttgtttca aagtttcaat aatatttgct cgtgttgctt ctgtaccgat
     4201 tccttctact tctttcaata tggattgtgc ttcttcgcta tccacggttt ttccagctgt
     4261 tttcatcgct gtgatcaacg taccttcggt ataaggttgt ggtggcttcg tttctttttc
     4321 aaggacttgt agatctaccg tcaccgcttc tccttttgtc actttaggaa gtagcggttc
     4381 ttcttccttt tcttttctgt cgctctcttt gccaaacagc tttttccaac ctagatctac
     4441 tggtactttt ccgactgtct ttagctgaag ctgctgaact tgcgtcaaga tcgttgtttc
     4501 ttcataggta tacttttccg caaacatggc caccgttgtt tttaacactt gttcataaat
     4561 agcctgctgc agtggactta attgttcaaa agccgctgcc gtcatgaccg tctttgtagg
     4621 aatgatcgcg taatgttcct ggactttctt attattcaca tatcgactat tcggtgtatg
     4681 aataggagta ggaatggctt cggcctttaa aaagtgtttg tattcgtcta agtgatccaa
     4741 cagatacgca tactcccctt ctgtaatata aggggtatct gttcgtgggt agcttagata
     4801 tttcccttca tacaaccctt gcatggcttc caaagtatct ttggcactag ctttcatgag
     4861 ctgattcatt ttagattgaa ggctggatag agaaaaaaga cgtggacttg ccgcactttt
     4921 tttctcggtt tcaacttggt ggatcgtacc tggctgtttt cccaattgaa cccccttttc
     4981 ttttaaataa tcttcaaatg cttcttgcgt cgcttgggtt tcattcggat caagcttcgc
     5041 atcaaatgcg ccattttgag cgattacttg ggcttttcct tcaaaatatg gttctttttg
     5101 gaagtttcta atcttttcct gtaattgata aatcatgtaa agcgttgggg tttggactct
     5161 gcctaaggaa aaacttcctg gtatgccttt ttgctgcaag agtaaactat acagcgggct
     5221 accgttcatg ccgatcaacc aatcggcgat ctggcgtgct tgtgcttctt caaattttgg
     5281 gtaggtctct tctgcatctc gtaactgctg gaatccctga taaatggctt ctttttctaa
     5341 ggagttgatc caaagtcttt taaatatctt tccatgatcg gcgcctgctt gttgaatgat
     5401 cgtccaagct atcagttccc cttcacgatc gctatctgtc gcaataataa tggtgtcggc
     5461 cgattgaagt tgctgtttca ccaccttgaa ttgctttttc ttatctttag gaacatgata
     5521 gtgataatgt gtagggaaaa taggcaaatg ctctaatgac cattgtttcc aattttcatc
     5581 gtacatatcg ggcgaatcta actcgactaa atgaccaaaa ccatacgtga tcacagtttc
     5641 tcctgaaaac aatcgatctt ggatctcaaa atacccatct tttcgggtag ctttagaaaa
     5701 gctatcggca taagccttcg cttgtgacgg tttttctgct aaaataacgg ttttcatcat
     5761 gacgatttca ctccctttct tgctgtctac cttgttgaaa caacatggca cgtttttgct
     5821 ttctgatctc ttgttggtag atttcatgct tcaattgatc attccaatcc ttttttactt
     5881 caggtacaaa ttcatgttca tgatttttat agcctttgta gtattgttct gctttataag
     5941 agaaaagaat ctgagcatta atatcccctt tttcttgata aaaacgatca aaatcgtacg
     6001 taccatccac gcgttcacaa gcttttaatt cttttgcgac ttctctacaa atatctttca
     6061 aattaatagc ttctattgaa tcgttaacag aaagctgctg accaaaagac gcatttactt
     6121 tgtgagcgga aaccaattgg ttcgttaatt gcagattgtt ttctgtcttg tgtatggcca
     6181 taatagcttc tggtgtgatc ttttcttctt ttgcgacttc ttcataaatg actttgtatt
     6241 cttttggcac ttgccaatca ttagcgattc ctcgttcaca acgtactttc tgattcgtaa
     6301 atggatcaac tgcgcccatt aattgttctt tttcgtagaa aatatgtcct gcacggtcat
     6361 tatccacaca aatctcgatg gattgaggaa aggcttgttt ctttctcaat tcagatactg
     6421 cctcaccaaa ataatgactg atcacatggt gttttaaccc ttccatcgaa actaaccacg
     6481 tatcgtttaa ctgatcacga tttagtgcag catagctaag taaatcaata ctactctcaa
     6541 aaaattttaa ctgcttaggg tcaccgatct ttaggttaaa gccatgattc gctgtcgaat
     6601 ttttatctat atgcttatat gtgcctctct caccaaaacg ttcataatca atgaacgttc
     6661 cttgataact cgcccctact gtctcatttg tctgtggatc tttccataaa aaggcgaccg
     6721 catgatctaa acgatcgtct cgaactaaga aattttgccg ttcatgtttt ggctttagtt
     6781 cggcaatcaa cccttctttt tctgcataat caatcagcca ctctggtagt tttcgttctt
     6841 ccttcagata attccttcct ttttctgtga gtggttgata atcaacgggg tgatctttgt
     6901 acttttctgt ctcaaaatga agattttcta ctcgttctgt tttgtcttca ttctttagaa
     6961 taaaatc
//
LOCUS       DAFGZE010000113         6853 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.117, whole genome shotgun sequence.
ACCESSION   DAFGZE010000113 DAFGZE010000000
VERSION     DAFGZE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.117"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            140..658
                     /locus_tag="LTU54_002776"
     CDS             140..658
                     /locus_tag="LTU54_002776"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBL8297425.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            786..1469
                     /locus_tag="LTU54_002777"
     CDS             786..1469
                     /locus_tag="LTU54_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBL8297426.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1778..1851
                     /locus_tag="LTU54_002778"
     tRNA            1778..1851
                     /locus_tag="LTU54_002778"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1812..1814,aa:Arg,seq:tct)
     gene            2010..2369
                     /locus_tag="LTU54_002779"
     CDS             2010..2369
                     /locus_tag="LTU54_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HBL8297427.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2492..4123
                     /locus_tag="LTU54_002780"
     CDS             2492..4123
                     /locus_tag="LTU54_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HBL8297428.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4185..4826)
                     /locus_tag="LTU54_002781"
     CDS             complement(4185..4826)
                     /locus_tag="LTU54_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBL8297429.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5012..5088
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5108..5416
                     /gene="rplU"
                     /locus_tag="LTU54_002782"
     CDS             5108..5416
                     /gene="rplU"
                     /locus_tag="LTU54_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBL8297430.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5443..5787
                     /locus_tag="LTU54_002783"
     CDS             5443..5787
                     /locus_tag="LTU54_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBL8297431.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5800..6093
                     /gene="rpmA"
                     /locus_tag="LTU54_002784"
     CDS             5800..6093
                     /gene="rpmA"
                     /locus_tag="LTU54_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBL8297432.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            complement(6186..>6853)
                     /locus_tag="LTU54_002785"
     CDS             complement(6186..>6853)
                     /locus_tag="LTU54_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8297433.1"
                     /translation="LIPKPQIKTEKKLKLYTKEQLELFLNEVSQEQNPYFKNRDYTLF
                     RLLAFSGCRIGEILALTWDNINFKTNEMAIKKTVARSDKYYISETPKTKKSNRIIYLD
                     EKTIKQLKFWKLEQRKYLFQLGFTKANYLFTNDENNFTINQSVAERYNIYRERAGLPY
                     IGLHGFRHTHASMLYEAGADHKEVQERMGHANIKTTMDTYTHITNSKKEETTQKLTNY
                     INF"
BASE COUNT         2116 a         1119 c         1420 g         2198 t
ORIGIN      
        1 aaaaatgaga taaagatgag gaaatagaat tagaaacata taattattaa agggaaacat
       61 ttttacagct ataatttgaa aaaaatttat cgttattata tgattaagaa ggattgaata
      121 aagaaagaag ttgaaaacga tacctgatta caaaaaaata acttttataa gttctataac
      181 gttgattata tttttaattc ttattcttct cgtcgtcttg gttccccgaa tttttggttt
      241 gactccttat attattgagg acaaagaaat gagtccatat tatgcacaag gaaatctgat
      301 atatgtacga ccgaaaaata caaatgagat tttagtcgga gatattatta catattatga
      361 aaattcagga aaaaaaatct cgacgcgtcg cgtaatagcc gttgaaaaca accacgaaga
      421 tttttataca aaagctgata caaagactta tattgagcct ggagtagtaa aaaagagaaa
      481 tattatcggt agtcccatct ttcagattcc atatattgga ctagtccttt caaaaacggc
      541 atttgcatgg attcaggggt tattctttac gattgctttt tgcctaacag gaatcacagc
      601 atggaatacc tttgtagagt tgaaaaagaa gcggagacga acacagaaat ttttgtaaga
      661 atgttaaaaa cataaatgta ttccagagat ccaacgactc ttctgaacat gaaacacgag
      721 aaaacactca tgcagcttgt agatttagtc agttgttcgt ataatgtgtg aagaggagga
      781 gtagtatgcg caatgaaatg atgcatcgtc ctgtagtcaa agaagaacta ttggatttta
      841 tgcgtaccca gcaaaaaaaa ttaccgggtg agctaggcaa actggaagaa gaagctcatg
      901 tagcagaagt accagttatt ccccatgaaa cagttgtatt tttgaaattt ttacttggac
      961 agcttcagcc tgaacgaatc ttggagatcg gagcagctat cggtttttca tcaagtgtca
     1021 tggccacgac aatcggagaa aacgctcatg ttacgacaat tgatcgtttc gatgtcatga
     1081 tcgagaaagc taaaaaaaac tatgaacgtc taggactgac agataaagtg actttgcttg
     1141 aaggtcaagc agcagatatc ttgccggagt tgtctggtcc gtatgacttt atttttatgg
     1201 atagtgcaaa atcaaaatat attgaatttt tacctgaatg cttacgtttg ttaagaaaag
     1261 gtggcgtttt gatggtagat gacatcttcc aaggaggaac gatcttgctc ccagatgaag
     1321 agattccccg tggcaaacga gctatccatc gtaaattaaa tgaatttctt cgtgttgtga
     1381 tggatcatcc cgatttgaca tcgacaatct tgccattagg tgatggtgtg atcttaatga
     1441 caaaggaatc agaaacaatc gagttgtgat acatgattat ggcagcctgt agataatcga
     1501 ttgaatcgag ggctcaattc gaacttgatg taaaagtttc tatatttgta agtcaattaa
     1561 aggatacagg aaatctaaat aaacaggagt agcatgaagt caatgtagct cctgttttca
     1621 attgataacc ttagaacagc tgaaggagaa tgtctcgttt taaaagattg atttagtagt
     1681 tgggccaaaa taattcatta tttgctaatt aaataactat ttcgtattga cagaagcaat
     1741 ttaattatct ataattattg atgttccgct gattcttgtc atagtagctc agctggatag
     1801 agcatccgcc ttctaagcgg acggtcgggg gttcgaatcc ctcctgtgac gctaaaaaac
     1861 cgttagaaac tgaggtttct aacggttttt ctttgttttt tgtgccaata ttgtttgttt
     1921 tcaaaggcaa ataaaataaa aaaacatcct tatcgagtac aattataagg agcaaaaaaa
     1981 taaaactcat cagaaaagag gaagaagata tgacgatact atctagaatt ttagtgactc
     2041 tagttgcaat tgaatttttt tatatcatgt atatcgaaac ggtgagaact gattctgaca
     2101 cgacaagtag ggtttttaaa atgagtaaag aagagttaag cagaaaatct gttcaaacat
     2161 tgtttaagaa ccaaggagtg tacaatggac tattaggagt gggactactt tatggagctt
     2221 atctttcttc cgcatctaaa gaaatcacaa gtatgctatt aatatctatt ttttttgttg
     2281 cattatatgg cagtttgact agcgatcgtt ggattttact caaacaaggt gggctagctc
     2341 tgttggcatt gctttctatt ttcttataaa tatagaaaaa ataagaattt atctaacagg
     2401 aaactttgtg aaaaatatta ctttatctca tgaaatatga tattctaatg aatggtattt
     2461 tagaaaattt catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa
     2521 aaaagaattt tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac
     2581 agtagctgct gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc
     2641 ggccgcaggt ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg
     2701 tttttatcaa atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc
     2761 aacaaaggga atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc
     2821 agcaggactt ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg
     2881 gtttacttca ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt
     2941 tcactcggaa ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc
     3001 gataaacaca gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc
     3061 aaaaaaatgg gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt
     3121 tatgctattg ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag
     3181 tagtaatcgt ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc
     3241 agctattagc attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg
     3301 ccgaatggca aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa
     3361 tctgttgctt cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt
     3421 ggccatcaaa tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact
     3481 gctctccatg cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg
     3541 tgttttgatt gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac
     3601 taataaatat tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt
     3661 tgatttaagt attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa
     3721 aagcgctgct atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga
     3781 aacggaaaag ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc
     3841 ttcagaaaaa ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc
     3901 catgcgaggc gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga
     3961 aaaagcagag caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa
     4021 acaactttct cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc
     4081 gttgacagct ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca
     4141 gacaagactg taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa
     4201 agtgaagcta gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa
     4261 atagctgctg gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca
     4321 tccttttgtt ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta
     4381 accgtatatt ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat
     4441 tttggctgga ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa
     4501 cgagtaggtg tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga
     4561 tgagtcacgc gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt
     4621 aagccagttg aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca
     4681 tgcgtatcaa tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga
     4741 ggtccttgcc cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct
     4801 tgtatcagtt cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata
     4861 aaagaaacac aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta
     4921 ttgcttgaat ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc
     4981 tggtaagcta gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa
     5041 taagcacagc atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct
     5101 ttatagcatg tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca
     5161 agcaatctat gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt
     5221 tatcttagta ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt
     5281 tgaaggtact gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc
     5341 taaaaaacat tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga
     5401 cgcgatcaac gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt
     5461 aagcgaaatg acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt
     5521 ccttatggaa gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac
     5581 gggattgcct cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt
     5641 tatctttata ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt
     5701 ctattagaaa atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc
     5761 caaattaaaa caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta
     5821 caattatttg cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca
     5881 aaacgtctag gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac
     5941 cgtcaacgcg gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact
     6001 ttatttgcaa aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa
     6061 gtgtctgttt atccagtagc tcaagaagct taataaataa actgctctat ctcttgtgta
     6121 gcaagggatg gagctttttt gtaaattatg ggattttttt tgaccatact tttgaccata
     6181 ctttcttaga agttaatata atttgttagt ttttgtgttg tttcttcttt tttgctgtta
     6241 gtaatgtgtg tatatgtgtc catagtagtt tttatatttg cgtggcccat tctttcttgg
     6301 acttctttgt gatctgcgcc tgcctcatat agcattgatg catgtgtatg tctaaaacca
     6361 tgcagaccga tataaggtaa gccggcacgc tcacgatata tattgtatct ttctgccact
     6421 gactgattaa ttgtgaaatt attttcgtca ttggtaaaca aataattagc tttagtaaat
     6481 cctaattgaa ataagtactt tctttgttcg agcttccaaa attttagttg ctttatagtt
     6541 ttttcatcta aataaattat tcgatttgat tttttggttt taggagtttc agatatataa
     6601 tatttatctg aacgagctac agttttttta atggccattt catttgtttt aaaattaata
     6661 ttgtcccaag tgagtgctaa tatttcgccg attctacatc cgctgaatgc taatagtcta
     6721 aagagcgtat agtctctgtt tttaaaatat ggattctgtt cttgagatac ttcatttaga
     6781 aataattcca actgttcttt tgtatataat tttaatttct tttctgtttt aatttgtggc
     6841 tttggaataa gta
//
LOCUS       DAFGZE010000114         6641 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.118, whole genome shotgun sequence.
ACCESSION   DAFGZE010000114 DAFGZE010000000
VERSION     DAFGZE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.118"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(99..1229)
                     /locus_tag="LTU54_002786"
     CDS             complement(99..1229)
                     /locus_tag="LTU54_002786"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBL8297434.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="LTU54_002787"
     CDS             complement(1878..3080)
                     /locus_tag="LTU54_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8297435.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="LTU54_002788"
     CDS             complement(3140..3343)
                     /locus_tag="LTU54_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HBL8297436.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="LTU54_002789"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="LTU54_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4880..5692
                     /locus_tag="LTU54_002790"
     CDS             4880..5692
                     /locus_tag="LTU54_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297437.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         2021 a         1334 c         1035 g         2251 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt t
//
LOCUS       DAFGZE010000115         6598 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.119, whole genome shotgun sequence.
ACCESSION   DAFGZE010000115 DAFGZE010000000
VERSION     DAFGZE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.119"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(142..468)
                     /locus_tag="LTU54_002791"
     CDS             complement(142..468)
                     /locus_tag="LTU54_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297438.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(505..870)
                     /locus_tag="LTU54_002792"
     CDS             complement(505..870)
                     /locus_tag="LTU54_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBL8297439.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(907..1122)
                     /locus_tag="LTU54_002793"
     CDS             complement(907..1122)
                     /locus_tag="LTU54_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297440.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            1251..1586
                     /locus_tag="LTU54_002794"
     CDS             1251..1586
                     /locus_tag="LTU54_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297441.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(1662..3659)
                     /locus_tag="LTU54_002795"
     CDS             complement(1662..3659)
                     /locus_tag="LTU54_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297442.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(3757..4032)
                     /locus_tag="LTU54_002796"
     CDS             complement(3757..4032)
                     /locus_tag="LTU54_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297443.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            4264..4998
                     /locus_tag="LTU54_002797"
     CDS             4264..4998
                     /locus_tag="LTU54_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297444.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            5037..6179
                     /locus_tag="LTU54_002798"
     CDS             5037..6179
                     /locus_tag="LTU54_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8297445.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
BASE COUNT         2244 a         1202 c          967 g         2185 t
ORIGIN      
        1 acactgctgt tataacagtg actaatgtaa agattttccc agcaatcatc agactattat
       61 ttacagggaa tcaaaaaaac tccttagtaa gcttacacct ttaggagttc ctgtttgaac
      121 ggtatgcata atacatttaa atcaatttaa cattaacacc tcagtatatg gtatctctac
      181 atatacacgg tctttttttg gatagtactc gacatttata ttgatagcac ttccattgtc
      241 taacgtagct ttagcccggt acgtgttatc accattatca tgagtgatcg tcacatcatc
      301 acatgtccga tttaagttat actgttcttg taaaatctga gttaccaatg gtacagatga
      361 cttctctagt ccagatgaac ccgtcattct tgaaaaaatt atacctataa tagccaatac
      421 aacgatacct ataatgacaa acttcttatt catatttact ttattcaact ttttatctcc
      481 tatctccaaa atgattttct ctcatcagta actcattgac atccccaata gtacaccaaa
      541 gatggctcca gctacccctg ctatcatcaa aatcgtaccc atttgttcat ctttctctcg
      601 atataagtaa cccataataa cagcaacagc accaaatacc acaggaataa atgctaatga
      661 tactaccata gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac
      721 gatttgttcc ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc
      781 ggttgtctca tctgtttctt gtaatgctcc gcaaaactgg caatacttac tttcatttgt
      841 tatttcttta ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct
      901 tattagctaa ctgtttactt tattaaatac atcagaaaac agctcattta ttggtacact
      961 taatatctca gaaagcattt tcatttgata gggtttagga atcgttctcc ctgtttgcca
     1021 cgaacttaca cattgctgtg taacattcat ttttttggct aactcagttt gggaaagtcc
     1081 tttttctttt ctgtatcggc tgagattact tacttttttc atgattctcc ccttctgttt
     1141 tattatatac aacaatatga tgtatttcaa ctcataaaat atataaatac ttcaaatgtc
     1201 attacaaata caacaacttg ttgtactcta ctgagaaggg gggtgtatta atgaaacaga
     1261 agcaatatgc aaaggtcatt agagaaaagc gaaaagcact cgggtggaca caaaaagaat
     1321 tagctaaaaa aatcttctct acacaacagg cagtagcaag atgggaaaat tctgtaacag
     1381 aacctaacct cgatagctta acagctcttt ctagagccct aggaacccct gtaagccatt
     1441 ttctagacaa tgttgacata gattatgagg aagagttttt ggctctctat cgctctctta
     1501 gtactgaaga tgcaattcga acaattgact atatgaagct attaaaaagg caagaaaatg
     1561 aacgcaatca gctattgaaa gattgaaaaa taaataaccc accgaacttt aacggtgggt
     1621 gtattgagat gaaataagat taagagctat ggataacctg tctagtttaa tataattggg
     1681 tctcttctat tccttactct ccgtccatca tctcttgtta cacttgtaaa ttgataaata
     1741 ggaactaatt caactcctga taatatcaat tcttttatta cttcatctat ccctgcccct
     1801 ttcgctagat atactaaaaa actactgctt gctttcacat tgtatagttt cttaatatcc
     1861 ttagaagctc gttcaagagt gaatccttgt ttaacaaagt agcctctgtt ttctattaaa
     1921 gcatttttca cgctgttatc cctgacattt cgtaggcgtt cacaaacagt gttaataagt
     1981 tctttttctg tcattagata tgtcccttgc cacgataagc cctgtaaaac ttcatgtgct
     2041 tcatctaaaa tcaattgttt ttccttgcta agttctgatc gggaattttg aggaaatact
     2101 cttttagcct ctttttcgtt aaaacatatc ttaacccatt cataagaaaa atattgatat
     2161 gtaaaatgaa atttttccag aagtggaagg atatcttcat tcaatttttg aatcacagaa
     2221 gttgcattaa aaacgtctaa tcgatagaca ttgcttctct tatgtttaaa gtttcctaac
     2281 gctttctctt cctttaacct atttaggtat tcgagcatct tcaaaggaac atcttcatca
     2341 tctaattttt ttatcaatcc tataaaagtc ataagtgata gtaacttatt acatttatct
     2401 atgtcataag ttttaccata gtaagaactc atttttttag ctaattgttc ccccgacaaa
     2461 atacaagtca cactttcctt actatcatca aaaaaattaa catcagaaag caagacatca
     2521 tagacacgtc gcatttcctc tagataattc cttattgtcc tgaaaagttt gtctttcatt
     2581 gattcaagag tgtcaaaaag tacttttgat tcctctttca attttcgaaa atgtgtagga
     2641 tacaagtcta ggttctctaa tagccaatta aatgtttcta cccgactgtc tttctttaaa
     2701 attagcatca caactccaat aaaattttca ctgatatcac aattactgga atgacaataa
     2761 aaaatggaat gcccagtttt actggtaaaa atactagcag agggattatg atcgttgtga
     2821 aacagacatc ttaagttttt acttgactta agtaatttat tcatatcaat ggttaacaat
     2881 ctttcataaa tctcattaaa agacatgtcg gaggaatcaa acaggcagtc tttaaaccac
     2941 tgtttcattt cttctttgtc gtcatttttt atcaacttaa caaaatcggt ttcattgact
     3001 agttcatact ctctccctga atcactttga caatttttag aacatgtcat ggtaaaatca
     3061 atcggaagct cagtaatatc taacatgttg tcttcattaa ttattatcac tgcatctgac
     3121 ttatatcctc caaaaaacat tctattcgaa gcatttacat tgtcatcaca cccggggatg
     3181 ttctttttca agtattcgta aacagcaacc attttaccat agtcagtaat tgcttctttt
     3241 aatagaaaga caatcttgta tttttctaga ttttcagagt accgaatact tttttgtatc
     3301 gcaaacgcag aattatgaac taaattgata gataaaacct tttctatgct taagtatcta
     3361 ctatcatctt tactcaaatc tactaccttc cccttctcat ctttaacagt gttatcgaca
     3421 tccagaataa ttactctggt ttccacaaaa ctctccttgt cattgccttt attctttgtc
     3481 ttgcttggaa taaatgattg tgatgaattt agtatctcta caaactcatc tcttgtaact
     3541 tctctatagt tatctaaaca cctatctctt acatcagttt tttctttttt tgtaactggc
     3601 ttctctaata atttttcttt atctacataa attggaatat taatcattga tgtttccatt
     3661 tgtgtttcct ccttataaaa ttctgttaaa gactaaaaca tacagataat aaaaacaaac
     3721 tctttattac tatatgtttt agtcttattt gtttactcaa cctttatacc gttccatgaa
     3781 actatcaata tcagattttt taaatctaat gaccgtatca atttttataa caggtaaccc
     3841 gtattgagaa acatattttc taatcgttcc tactgataca cctagatatt cacttgcctg
     3901 ctgatagtcc atatactcta gagtgactcc agaaataatg tcttctagca taggctttag
     3961 cactgtaatt atctctttca cttgttgttc aacttgtata gaaacaatgc tatcaatgat
     4021 ttgtagagac atatttcccc ctcctttcgt gattgcttga acaaattttc taagaattat
     4081 cttcgtattt tttgctcaaa ataaaagtat tttgttatta accgattaat caaacaaaaa
     4141 atgattacca gttcatcgac atacacataa tacctcgata aaaagggggt gtcaatagtt
     4201 ttgtaaaatt tatttacaac accccttttt tgaggtatac taattttagg aggtgtggtc
     4261 aatatgataa aacgtccacc aataaattat ttagaacgaa aaaagatact cggaacaaaa
     4321 ataaaagcta taagaaagag taaaaaatta acacaaccag catttggtct tatgattaac
     4381 aacggtcaat taattgacaa aaagactatc tacgaatggg agaagggaac ctatttacca
     4441 ataccagagc gattatcccg aattgctgat ttgggaaata tgagtataga ggagttagtt
     4501 tgtggcaatg tagaagaata tattttggga attattcttt atagagatag cattgtacta
     4561 gatggtataa catttccaga taaaaacctg ttccagcatc ttcgtcaaca gttcccacca
     4621 gtacactcta atttagatac atggctggat agatattcca aattagaacc agagatgcaa
     4681 gaatttattg ctaacaagac ttgtaataaa gttaaaaatg aaaaaattag tcttttcaat
     4741 atcttaaaaa tagaagaatt attcatcaat gctattgtag aagaatttga caataacatt
     4801 cttttcctca ctagcagtat agaagaacta ctagaaagaa tggtagatga atggctacct
     4861 attcaattga aagacatgtc ttatcccgaa gaagctgtga gagaaattac tgataatata
     4921 aacaagctag aacaaacgat ttcatcaatt gggaaaaaat acactaagaa aaaaatgaag
     4981 ggaggtgata ccatctagtg gtaagtactc cttgtaaact aagaggagat gatttcatgg
     5041 caaaaatatc aaagtacaaa aagaaaaacg gtcaagtggc gtggatgtat aaaggacaca
     5101 ttgccaccga tccacgaact ggtgaaaaaa taaatactac gagaagaggt tttagtagca
     5161 aacaagaagc tcaaaaagat tatgacgagt acagacatca aatattatac ggtgttaaaa
     5221 agaaagcacc tgatatgaca tttgaagacc tgtataatga atggataact catcagcgta
     5281 ccagtgttaa agcttcaaca atcgcaatct cagtaagata cgcaaataat caaatacttc
     5341 cagcatttgg taagcttaaa ctatcgaata tctctgtccc atattgtcaa aaagtggtag
     5401 atgaatggca cagtaaatat gaaagttacg attatatgag aaaacagact gcacaaattt
     5461 tacgctacgg ggttgcaatg caatatattg acaataatcc tatggaaaaa acacttcttc
     5521 cacgaaaaaa agagtatgag aaaaatcgta aattttactc aaaagaggag cttaataatt
     5581 tactggatgc ttttaaagat tttggtaaca tgaaacaata cgccttcttc agattattgg
     5641 catacacagg tatgagaaag agtgaagtac tcgctttaca gtggaaagac atagacacgt
     5701 ttaataaaga actacatgtc aataaaactc ttgctgtaga tgaatttggt aaagttataa
     5761 ttcaatctcc gaaaacaaga gcttctagac gagttatctc tttagatact gagactcttt
     5821 ctatcctcaa caactggaaa ttacagcaaa aagaagagta tttaaaactt ggatacaata
     5881 ctagctctaa agagcaacat gtttttacaa cagttaaaaa cactctatac attccaaaca
     5941 ctgttaatga ttggttaaga tatattttga aaaaatataa cttgcctcgt attacacctc
     6001 atgggtttag acatacacat gctagtttat tattagaagc aggagaatct gtcaaggtag
     6061 ttcaacaacg attagggcat gaaaatagta aagtgacatt agacatctat gcacatatta
     6121 caaataatgc acctaagaaa acaggtcaag actttgctga tatgatggct catcagtaaa
     6181 agagctggtc aaaatctggt catttctagt atacaggcaa aacaaaaaag ctagaaccct
     6241 tatatatcaa gggttctagc tcctataaca ggtttaccca acactgcctt ccaatgactc
     6301 ttttgggaat cagaggaaag aatcaggctt tatagagata tttcatctaa caaattactt
     6361 tagactgaca gtatatatca gcaggtactg tcatcgggaa cgtagccaat atcgtatcca
     6421 aaacagcagg tagagaagct atttgaggat tcaagttatt ctatttcaac catgaagtta
     6481 agttcattaa aaataaagat agttggtatt tcaataattt ttgtatagag ttaatcactt
     6541 tattgatgct aattcatctt gattggtatt ataatcgtat tcaagttgcg tggcgtgc
//
LOCUS       DAFGZE010000116         6463 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.120, whole genome shotgun sequence.
ACCESSION   DAFGZE010000116 DAFGZE010000000
VERSION     DAFGZE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.120"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..142)
                     /locus_tag="LTU54_002799"
     CDS             complement(<1..142)
                     /locus_tag="LTU54_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297446.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQ"
     gene            complement(183..437)
                     /locus_tag="LTU54_002800"
     CDS             complement(183..437)
                     /locus_tag="LTU54_002800"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8297447.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(548..802)
                     /locus_tag="LTU54_002801"
     CDS             complement(548..802)
                     /locus_tag="LTU54_002801"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297448.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(995..1270)
                     /locus_tag="LTU54_002802"
     CDS             complement(995..1270)
                     /locus_tag="LTU54_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HBL8297449.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1242..2195)
                     /locus_tag="LTU54_002803"
     CDS             complement(1242..2195)
                     /locus_tag="LTU54_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL8297450.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2807..4300
                     /locus_tag="LTU54_002804"
     CDS             2807..4300
                     /locus_tag="LTU54_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HBL8297451.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4435..4731
                     /locus_tag="LTU54_002805"
     CDS             4435..4731
                     /locus_tag="LTU54_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL8297452.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4755..5714)
                     /locus_tag="LTU54_002806"
     CDS             complement(4755..5714)
                     /locus_tag="LTU54_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HBL8297453.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
     gene            5897..>6463
                     /locus_tag="LTU54_002807"
     CDS             5897..>6463
                     /locus_tag="LTU54_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297454.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGY"
BASE COUNT         2070 a         1089 c         1134 g         2170 t
ORIGIN      
        1 attgctttac ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta
       61 cacgtggctt tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag
      121 ttcttcgtgt tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca
      181 ccttaatttt tctgtctagt taattgtagc ctatccatga tcggtttatt agacctttga
      241 ggtctatatt ttttaactac aatcgacctt aaatttaatt gtttcattag cttttggaca
      301 tgtttaagag atactgaaat cccttctttt aataaaactt gatggatctt tgtggcacca
      361 tagcgttgtt tattcgtaaa aaaaattgtt gaaatcttct ttttcaactt attatttctg
      421 atttctgtag ctgtcaacgg tttattctta taaaaataat agacacttct agggatttct
      481 aataaccgac acaacaaaga aacagtgtaa tccttacacc atttttcaat aaattgaaca
      541 agcgaattta ggttttccga gagaatatgg ctgcggcttt ttttaagatt tcaagttcct
      601 catttaaacg agcattttct tttctcagtt cagctacttc ttttccagtc agtcctgtgg
      661 actgatcagg taaatataaa ttcttccatt tgtaaatcgt tgcttcagat aagccatatt
      721 ctttcgctaa ccctcgaaca gaacgtccag tttgattcag ttcaacaatc atttgtttaa
      781 aattttcttc atatcgggtc ataaaaattc atcctctcgt agataagttt aatttattct
      841 tgtctctacg aaaagctgtc taagatttga tactaacacc aaatgttgta tacccccaaa
      901 caatacacat attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa
      961 atatatttac tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata
     1021 taatccgttc ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt
     1081 catacatagg aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat
     1141 ctacttgaac tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa
     1201 tatcatcttt aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta
     1261 ataatgccat ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca
     1321 gcatatttag gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt
     1381 ctttcttcta attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa
     1441 tcatttaatt ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg
     1501 cttttaaaaa tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga
     1561 gaggaacgtc gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct
     1621 tcaaaattcg tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt
     1681 gcgtaccgtt ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga
     1741 accacattat ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat
     1801 tcttctaaag gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac
     1861 aagtattcat taagttcttc agatccagga gagccagcca taatatataa atgtggactt
     1921 acttgagtaa tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca
     1981 aagctagtaa tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa
     2041 tcaattagca acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg
     2101 tctgtagtct taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta
     2161 tctgatgttt taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa
     2221 atatatttag tatacttcat atacttaata tattcgatat atctaatata ctccaaattg
     2281 ataagaattc agactttata aggaatatag tggatatact aaatatattt gctatattca
     2341 gtatatcaga tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag
     2401 agtgaaaaga ggtaaactta attctataga aaccacagat aattcactat ttacaaagta
     2461 gcaaaagcag tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat
     2521 tgattttagc agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt
     2581 ctcaagaata tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc
     2641 cgattgcctt tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt
     2701 gtacttaaat taaatattct taaacactga agagagaaca catactatgg ctgagattaa
     2761 agctaaactg tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat
     2821 ttattcagct attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa
     2881 acctgcaggt catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca
     2941 attaatgaat gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga
     3001 aaagaaacta acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta
     3061 tcgaacagtg gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt
     3121 tgatatcgat tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt
     3181 gggctacatg ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact
     3241 aaagacacca ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa
     3301 aatttcaaaa acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg
     3361 taaccacttc ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa
     3421 ctatcagtat tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa
     3481 aacaaaagaa gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca
     3541 agtagatgaa ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg
     3601 acgtcttggt cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta
     3661 tgcattggat acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct
     3721 atctgaagca gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc
     3781 aagcagagaa ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa
     3841 tgatctgttc attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa
     3901 atatagtcat aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct
     3961 tgctagtgag ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc
     4021 caagcgaaca cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa
     4081 ggtaacacga ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag
     4141 ggttattcaa ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt
     4201 tggactcaac gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc
     4261 tcttaaagaa gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg
     4321 ccttatacaa tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt
     4381 aggtggtata cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg
     4441 ttaaaaaatt atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta
     4501 tctgtggaag tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt
     4561 accagggaaa aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct
     4621 tctggggaga ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt
     4681 gagtataaaa tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta
     4741 tttactctta attgtcaaat taagttagac aaatcatcaa tacttacgta actcaaaaat
     4801 acttccaaag gtgttcgata attcagtgat tttctaggaa tattatttcg tttcgatgcg
     4861 acagattgaa taaaagattc atctactgaa ttgaaatcca tagatttcaa taaaccatct
     4921 ctacgtaaca atccgttaga attctcgttt aggcctcttt gagacggcgt tcctggatca
     4981 gcgaaataaa tggcaatatc attggcatta ctgatctgtt tccaatttga aaattccttt
     5041 ccacaatcaa aagtgatgga tttgaataga tttttcggta cagattcaaa ccaatgattt
     5101 aatttgtttt caatatcaat cgcttgtcta ccacacggtt tcaatgtgat gataactttt
     5161 gataatcgct caactaaggt aattacagca cttttatgtt tcaaacctac gatagtgtca
     5221 ccttcaaggt gaccaaactc atttgagaat tggctataat ctttttcacg ttcatgaata
     5281 gagcgacgga aagcttgttt tccacgtctt tcttgatgtc catttggttt acgcttgcct
     5341 ttcatcggta agtcagaagg attaaatagc ccctttttga acatacgata aatggtacga
     5401 gcagaacaac gaataggaaa cgctgcacga ccaacaatca catcgggtgt ccatccttga
     5461 acaacctttc tttgaatata ttctgattgt tcctcaggta aaacaagcgg gcgtctacca
     5521 cagtttgatt tgtttttctt atactgttga tagtactcta aggctgattt tccttgcttg
     5581 aagaacaggt atactttatg gatcgtttgt cttgaacgat tcaagaaatg cgcagttttc
     5641 gcaacagatt gatttatttt gaaataagac tctatcatta caagttcatc cgttgtaaga
     5701 tgggtatagg tcatttgtac tcactcctta taattctttg gacggaacta ttttgagtgt
     5761 agcacaaatg acttttttag ttgtctagct taattttaca atcggcgtac ttaaaaaatt
     5821 ggttcattga gcaacctatc gctgctaaag tagcaaagga aaacgttaaa ggaaagaatt
     5881 tagggaagaa atcgaaatga aatcggacga ctggattgaa cacacctggc ttcctctgaa
     5941 aagaaatagc aaacgaaaga aattttgggt attgcaatta tggccattag gaaaactttg
     6001 tagttacggg gcacgactta ttcttgattt aaccgtttac tcgattggag tatttggaac
     6061 ggcttattac ttgataaatg atcccaatag gattgcagca tcagacattt ttacagtgct
     6121 aatgacgttg atttttgtca tgctattgtc tatttttact caattagtca tgcaatttac
     6181 caggcaaaaa ttaacagcag gaaaactttt caaaagccta ttcgatttaa ttgttgtaaa
     6241 tgcaattcta gcgactttaa taaaacaaat tgaacaatct ggaagaacaa caaaaggagt
     6301 gaaggatttt ttattgacgt ttattcactt tgtaaatgga cacatgtatt tgtttgaagt
     6361 gggtattatt ctcttttttg tcgggtatgt atccacgaaa cttattaaac gttcaattga
     6421 aaaagaaatt gaaattgacc tgattccgac acttcttggt tat
//
LOCUS       DAFGZE010000117         6421 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.121, whole genome shotgun sequence.
ACCESSION   DAFGZE010000117 DAFGZE010000000
VERSION     DAFGZE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.121"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..1304
                     /locus_tag="LTU54_002808"
     CDS             <1..1304
                     /locus_tag="LTU54_002808"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL8297455.1"
                     /translation="FNVLADQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSK
                     KLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTP
                     HMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTT
                     YNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFL
                     DDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLL
                     EDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEE
                     NIQKILETYAERKDVEKYAHLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDI
                     KKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHE"
     gene            1297..2541
                     /locus_tag="LTU54_002809"
     CDS             1297..2541
                     /locus_tag="LTU54_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL8297456.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLDDTIA
                     LHQRKLDLLKETKKGFLQKMFV"
     gene            2555..5704
                     /locus_tag="LTU54_002810"
     CDS             2555..5704
                     /locus_tag="LTU54_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL8297457.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYAQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         2283 a         1055 c         1321 g         1762 t
ORIGIN      
        1 ttttcaacgt cttagcggat caagcaaaac aagcaacgtt ccaattgaat gatctaaaca
       61 aagcctttgt gcaattggca tctacctaca atcaatttaa tgggttattt gatgatgtgg
      121 atctccaatc aaaaaaacta ggaacagatg agcaacaacg aaatgtgacg attaccgaag
      181 tcattaagaa attgaatgac gtggaagttt taggtcacga tggggacgtg atcggggatg
      241 cttatgaatt tctgatcagt caatttgctt cagaagcagg gaagaaagcc ggagaatttt
      301 acacgcctca tatggtttct gatatgatgg cacaaatcgt gacgttggat caaaaagaac
      361 gacgattctt tagtgttttt gatccaacca tgggttcagg ttcattgatg ttaaatgtgc
      421 gtaactactt aactcaccca gataacgtta aatatcatgg gcaagaatta aatacaacga
      481 catacaatct agcaaaaatg aacttgattt tacatggggt agatgccgaa gaaatgaatc
      541 tccgcaatgg ggatacgttg aataaggatt ggccaacaga tgaaccttac acatttgatg
      601 cagtagtaat gaacccacct tattcggcta attggtcagc agatacgacc ttcttagacg
      661 attctcggtt taatcgttat ggaaaattag cgccaaaatc caaagcagat tttgcgtttc
      721 ttcttcatgg tttctatcat ttgaaggaaa cagggactat ggccatcgtc ttgccacacg
      781 gagttttatt ccgtggcgct gcagaagggg tcattcgtca aaaattacta gaagacggca
      841 gtatttatgc tgtgatcggt atgcctgcga acttgttctt cgggacatcg attccaacga
      901 cggtgattgt attgaaaaag aaccgccaaa ctcgagacgt attattcatt gacgctagtc
      961 gagaatttgt caaaggaaaa aatcagaata aattatcgga agaaaacatt caaaaaatcc
     1021 tcgaaaccta tgctgaaaga aaagatgtcg agaaatacgc tcatttggca aactttgatg
     1081 aaatcaaaga aaatgactat aacttaaata ttccacgcta tgtagatacg tttgaagaag
     1141 aagaaccaat tgatatggtt catgtcggaa atgacatcaa gaagatacga caagaacagc
     1201 aagtgctaga aaaagaactt ttagaagcta tctcttccct gcaaacaaca cctgaaaatg
     1261 aagcgtggtt acaaggtgcg ctggaggtgt tcaaacatga gtaatgatat acaaccagaa
     1321 attcgtttcc caggatttac ggaagcttgg gaacagcgta agtttgaatg cttacttgat
     1381 aaaaaagatg gagtaaggag gggaccattt gggagtgcct taaagaaaga attctttgta
     1441 tctaatagca attttgttgt ctatgaacaa caaaatgcta tttatgataa ttacgaaaca
     1501 agatataaaa ttactgaaaa aaaatataat gaattaatca aatttaaatt agaaccaggc
     1561 gattttatca tgagtggcgc tggaacaatt gggagaattt caagagtacc aaagcaaata
     1621 aaaccgggtg tattcaatca ggctttgatt agacttagaa taaataaaga aatcacagat
     1681 tcagaatatt ttattcaatt tattcgtgca gattttatgc aaagaaaact cacaggagct
     1741 aatccaggtt cagcaattac aaatctcgta ccaatgtcag aagtgaaaaa atggatagta
     1801 caatttccaa tattagaaga acaaaagaaa attgggaact tcttcaaaca actggacgac
     1861 actatcgctc ttcaacaacg taagctagat ttattaaagg aaacgaaaaa aggattctta
     1921 caaaaaatgt tccctaaaaa cggagcaaaa gtgccagaaa ttcggtttcc tggatttacg
     1981 gaagattggg aacagcgtaa gttcaaagag tttagtaaga agactggaaa aaagaacacg
     2041 aaagatttag acttccctgc ctattcggta agtaataaag caggtttaat ttctcaaaca
     2101 gaacagtttg atggtagtag gttggatgat ttagaaaaaa caaattataa attcgttgaa
     2161 ccaaatgaat tcgcctataa tccagcaaga gtaaacgtag gatcaattgc atttaacaat
     2221 cttggtatga cggtcattgt tagctctctt tatgtagttg tgaaaatgag cgaggactta
     2281 gataatgagt tcatacttca gtttatcaag tcaccaacat ttatcaaaga ggtcaagcgc
     2341 aacactgaag gaagcgtacg agaatactta ttctatgaga atttcgctaa tatcaagttt
     2401 ccttttacta ggaataaaga ggaacaacaa aaaatcggcg ctttcttcaa acaactcgac
     2461 gacacgatcg ctcttcatca acgtaagtta gatttgctga aggaaacgaa aaaaggcttc
     2521 ttacaaaaga tgtttgttta gggagggaag ataaatgaaa attagtcacc gtgaggaagc
     2581 ggaagtcgaa gaacagttga tccgtgtatt gggggaaggg cataatcagt ggacgtatcg
     2641 tcccgatcta aaatcagaag aagatctttg ggtcaattta cgccaaaaaa tcatctcaaa
     2701 taatcaagca gaactgaatg attttccttt gaccgataaa gaatttgaaa cgatcaaaac
     2761 agaattgtta ctacgaacga aaaccccctt tgatgctgcg aaatggttaa aaggtgaaaa
     2821 tggaatggct cggatcacga ttgaacggga agatccgcaa ctgggttctg tctcgctcat
     2881 tctttattct aatcaagaca ttggtggagg aatctctacc tatgaagtgg tccatcaaat
     2941 tgcaaaacgt ggaagtaaca tagaagcacg ggatcgacgt tttgacgtca ccttactgat
     3001 caatggatta ccgattgtcc aaatcgagct gaaacaagta accgcaaaag atagttttta
     3061 tcaggcgttt aaccaaatca aaaaatatgc agaagaaggt atgtttcgga acaatatctt
     3121 ttcgacctta cagttgtttg tggtttccaa tgaacaaacc actcggtatt ttgcaaatgc
     3181 cttaccaaag gatttgcatc ctaagttttt atttagttgg cgaacgaaag ataataaaaa
     3241 ggtagaaaac ttatatgagt tttgcaaaca agtgctaaat atcccggatg ctcatcgctt
     3301 gattgcggat tatacgattg tcagtgaaga tcaagacaat aaaatcctca tggtgttaca
     3361 tccttatcaa gtacatgcga ttcaagcctt gtttattgct gcaaataaac atcaatcagg
     3421 atatgtgtgg catgcgactg gttcaggaaa aacgttgact agttttgtat ctactaagct
     3481 tttagcacga aaatcgggga tcgatcggac gatcatgtta gtggaccgca aagatttaga
     3541 taatcaaacc accacagagt ttacgaaatt tgcttcggaa tttaatactg ggatttcttc
     3601 cgggaacgcc aaagcgaata gcttgatcgt gggaacagga agtgccaaag agttaagtga
     3661 aactctgttg gcagatgcca atgcaaatgt tgtgattatt acaacccgtc aaaagctaga
     3721 tgcggcgttg aaatatgcaa aaaaacaaga agagaaaaaa ggaaccaatc gcttccaaaa
     3781 attaatgggg caacatatcg tgtttgtagt agatgagtgt catcgagcgt taagtgctga
     3841 aaatatggaa gaaatcaaaa aaatgtttcc gaaatccact tggtttggct ttacaggtac
     3901 accgattttc cctgagaatc ggaaacaagc caaagggcaa ttggcacgta cgacccatga
     3961 tcagtatgga gaggtcttgc atacctatac catcaagaat gcattagaag atggctcggt
     4021 tttaggattt caagtagagc atgaaaacac cgtggagccg acttcattag agaataaaat
     4081 ctatcggaaa ttaaaagaag tagaaacata tgcagagtat tcaccggaac agatcaatcg
     4141 tatgattgat caaatggagc ctgtcaaaaa agaaagttat ttggacccta ctgtttttga
     4201 agcagatgaa catatccaaa aagtcattca taaaatgttc cgtccagaca atgcctatac
     4261 aaaatttgat tttcgaaatg gccgtccaac gaagtctgcg atcttaacca cgagttccat
     4321 tgacatggcc aaaaagtatt atcgagcgat caaagaaatg acgaaagaac ctgactggtt
     4381 aacaaaagag tttcctaatc agcccattcg tgaaggaaga acgatggagg atcctgattt
     4441 tccacgaatc gctattacct attcattaga agaaaacagc cgagattcaa gtgtccaaca
     4501 agaagaaatg caaaaaatta ttgaggaata taatcagtat tatgatactt cctggtcatt
     4561 agcagacatt gagcgttata acggagacat caataatcgt ttggcacgaa aaagagcgga
     4621 attcaaacaa tttgggaaac aaattgattt agtgatcgta gtggatcgtc tattgacagg
     4681 ttttgacgca cctacgattc aaaccctatt tgtggatcgt aatctagagt atgcaggatt
     4741 gatccaagct ttttcacgta cgaaccgtac gtatccagaa aaaacaaaag gactaattgt
     4801 tactttccga aagccagcca caatggagaa gaatgtcgag gatgcgacaa aactgtattc
     4861 agaagcaaaa gaagaatcgg gtttgattta tccaagttat caggaatcca aaaaaagatt
     4921 caagcaagca tatggtaagt taaaagaggt ttcatccatg gaatccattg atgaacatac
     4981 gccacttgaa atccgtatcg agtacgtcaa agcattccaa gaattgaatc gtgcttatga
     5041 atcgttagtg acttatgacg attacaatga tgacatggtg aaatcttcaa ccttgaacaa
     5101 tcaaattcaa cttttggaag aacaaattgg cgtgtacgag accgtgaagg gttcattaat
     5161 agaggaagaa ccagaaaaag agggagaaga tttttccacg atcgaatttt atagtgaaaa
     5221 taccacgaag ttgtatgata ttgattctac ttatattgat caattactag gaacttatgc
     5281 ggcgaatagt tcagatgcac gagaagaaat tgaaaaagct ttggctaaat tgaataaaac
     5341 agaaggagtc aaagaagttt accgacaaat tttaaatgcg tttgatgtgg ggactttaga
     5401 tagtaatgaa gatatctttg tcattaaacg tcgctatttt acacaagcga gtgatgattt
     5461 gattcatgtc ttttccaatg agtggtttgt ttctgagaaa gaattacatg cttctaatct
     5521 gcaatatatg ccaggtgaag atccaattcc taatatgaaa gcaattatca acagtaagga
     5581 ttacgaagga tataaagcca accatccaga agcgaagcca tttaaatacg cacaagaaat
     5641 gaagcgagca tggcgtaaaa tgttagatga tgagctgatt ccacttgaaa atgaattgag
     5701 gtaaacgaga agagttaaga aaattgtacg attcaaagat ggtagtaaag tggaagtgta
     5761 gaaaataggg atagatgtct tttatttgtt cacattttat aacgaagtat ttaagacagc
     5821 tattgaataa tgtttcctcc acctttttca ttcatttacc ccaccccgtt gttatatgac
     5881 cgtgttttga gaggaaccaa taaaaagcat aggctaacta ggggttcctt tttcttttgt
     5941 ttttttggaa tccgcttaag tgaaatgtat ctgaaatgaa acggagataa agtgattttt
     6001 tgctgtaatg acaaggtttt agctgtttca gataccatgt aacccaaaat agtttattga
     6061 gaaaatagga gagtatttga tactaactag taggaaaaaa tgagataaaa agtaaatgga
     6121 atcatttcag gtaatctcaa ataaaaaaat atacagtcct gtggcacttt acctatggga
     6181 ctgtatatat tttttactct cagcgggctt gttttgactc cgtgtctgac tccgtcgtat
     6241 tgattttaaa tgatattcga tgaccattta acaggtcatt tttgaaattt ttgtctctat
     6301 atgactcagt ttgataatta ttgatagatc aatttgggtc tatatcgaac aagggaataa
     6361 tccaataaaa aatgatttag aatatcaaaa taacaaaaaa gaggactacc tgtattagtc
     6421 c
//
LOCUS       DAFGZE010000118         6392 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.122, whole genome shotgun sequence.
ACCESSION   DAFGZE010000118 DAFGZE010000000
VERSION     DAFGZE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.122"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..382
                     /locus_tag="LTU54_002811"
     CDS             <1..382
                     /locus_tag="LTU54_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HBL8297458.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="LTU54_002812"
     CDS             complement(776..1783)
                     /locus_tag="LTU54_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8297459.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="LTU54_002813"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="LTU54_002813"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HBL8297460.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="LTU54_002814"
     CDS             3679..4002
                     /locus_tag="LTU54_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBL8297461.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="LTU54_002815"
     CDS             4013..5695
                     /locus_tag="LTU54_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HBL8297462.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6392
                     /locus_tag="LTU54_002816"
                     /pseudo
     CDS             5901..6392
                     /locus_tag="LTU54_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         2034 a          981 c         1294 g         2083 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga ag
//
LOCUS       DAFGZE010000119         6361 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.123, whole genome shotgun sequence.
ACCESSION   DAFGZE010000119 DAFGZE010000000
VERSION     DAFGZE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.123"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..1169)
                     /locus_tag="LTU54_002817"
     CDS             complement(<1..1169)
                     /locus_tag="LTU54_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL8297463.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWG"
     gene            complement(1222..1893)
                     /locus_tag="LTU54_002818"
     CDS             complement(1222..1893)
                     /locus_tag="LTU54_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL8297464.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(1975..3141)
                     /locus_tag="LTU54_002819"
     CDS             complement(1975..3141)
                     /locus_tag="LTU54_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HBL8297465.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(3147..3836)
                     /locus_tag="LTU54_002820"
     CDS             complement(3147..3836)
                     /locus_tag="LTU54_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HBL8297466.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGYQKDKIAEKVDGVTK
                     TIHIQEQAKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYLDTEMGNGNYILAGYNVDTPSVLLSDMTDLSEGEEIDLIDA
                     HYKYQYEVSKRGKISDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(3963..4202)
                     /locus_tag="LTU54_002821"
     CDS             complement(3963..4202)
                     /locus_tag="LTU54_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297467.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKMMKQKHLKRLSVMQYINDMKIT
                     GKEKACLLGSMKNFEQLRRTYVKTGSNCQLLLEVS"
     gene            complement(4269..4508)
                     /locus_tag="LTU54_002822"
     CDS             complement(4269..4508)
                     /locus_tag="LTU54_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297468.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            complement(4622..4963)
                     /locus_tag="LTU54_002823"
     CDS             complement(4622..4963)
                     /locus_tag="LTU54_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL8297469.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLTDDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            complement(4950..5279)
                     /locus_tag="LTU54_002824"
     CDS             complement(4950..5279)
                     /locus_tag="LTU54_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297470.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            complement(5870..6202)
                     /locus_tag="LTU54_002825"
     CDS             complement(5870..6202)
                     /locus_tag="LTU54_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL8297471.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
BASE COUNT         1888 a         1334 c          907 g         2232 t
ORIGIN      
        1 tcgccccatt taaatacact gtaaggagaa gtttcacttt ttacaccatt tactgtaatc
       61 ccatctgttc cacctgcgat atctgtaggg atatgaattg taccttgata attcactgcg
      121 tcaccaattt ggaaagaatg ttcttctcct gttccatttg tcactgtttt atcgatcact
      181 ggtgctttat agttcacgta ggaagctttt aaatcacttg tcacgccgtc attcgtaatt
      241 tcaaaggtta acttattgtg cgcatcgttg cgagcgtctg cgcctaacaa atattggtct
      301 gctgttggtt cttgatctga tccaactaca acttctgaag gaacttccac aaagtagtat
      361 tttcctaacg tcaaatcagt tgcagtcagc tttccttgat catccgatac aagctctcct
      421 aattttgttc cttttccagg ttcgcccttg tataactcaa attttgcccc ttttaaaggt
      481 gtttgtttgc tttgagctgt gccatcgtca ccaaatttcg ttaattcaaa actcaactta
      541 gaaacaatgt tctttggata caaatgaata tcttttaaaa atcctgatcc tgtgttgtct
      601 gtcattggcg caatcacaac cattggtttt gctttttgtg tgactaatcc agcagcggat
      661 ttactttcat aaaccacata tacattccct ttggaattga cataggctgg ttgatcagca
      721 aaagtgatct caccattttc atctacagct gaagtaattt tattggttgc attcttaacg
      781 tactcactgt tagatccctt gtcttctaca tctttaacga tttcgtccac tttcgcgttg
      841 gttagatcag aatcttcagt cggtaatact tgatccgtaa tatttcctaa cgtaaactca
      901 acatcaccat aatctgcctt gttgtatttc gttacacctg taggatattg atcgtgcgta
      961 taaccatcat ttttgattcc gtcaatatca acgttcaatt gcgtactttg atcgtacatt
     1021 aatttatgaa ccgtgatatt gaccctttct ggtggcgttt tttgcgtgac atcttctgca
     1081 taagctcgat acccctgaaa gcagcctgca atgattccta acattccaac aatcattcca
     1141 gcttttttta cataccgatt tattgccatt ttttactctc ctcatttcat atttcaacga
     1201 ctcgcgttga ttaaattctt gttattgtag ctctccctgt accacgtatc tttcttttcc
     1261 ttgtgacgta taaacacaag taaacaacgt taataagggt ttctctcctt tctttggtaa
     1321 agacaagaaa gagttctctt caggaacgcc atcgattaat ttaaagtact caggtacgat
     1381 aaatttacgc gttaccttgt aacgatagac gccatcgtta ctaactaagt ccatcacctc
     1441 ccctaatgac aacttttgaa tatcagaaaa taaaacacct ggcatttcca tgttatgacc
     1501 tgctaggaca tagtttcctt tacccatttc ggcatcttca taaaaataag tagccgctcc
     1561 aagactcaaa gtgtattgat ttgccccttt aaaaattggc aatgctaaac caagtgcagg
     1621 aatctgaatt tgtgcaaata cttgtttatt gacgcccatt tcgattgctt ctttttgtat
     1681 ggacaacatt tgccccaaag acatatcatt attgacgttc gttgcatcct ccttataact
     1741 ctttgcgtgt gcattgattt gatctacggg catctttttt tctatatttt gcttaaacac
     1801 ctctccttca tgtttttggt agctaacata gccaaacaca ctgcaaacga cgattaaagc
     1861 gactcctaaa aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa
     1921 aaaacacttt ttcacaacga aaaagtgttt tctcttatta ctattctatt tcatttatct
     1981 ggtggtttta tattggtgtg tagccattgc taacattaag aatacaattg gcacccaact
     2041 gattgtcgtt aatgtttctt ccatgaacga ttgacacatg atcgctaaaa aagcccacaa
     2101 gaatgttagt tgtccatttg tcagctcacg ttttcttacg agcaaccata agaaactaag
     2161 caaaagaata atcgatagaa caaaaccaat caatccatag tgattgacga gtgcgtgata
     2221 cgtattatga ttacctagaa tctgatttcc tctatggaaa aagaaaggtt tcatccctac
     2281 aaatatttgt cctgtttttt cgcctaatgt ttcatagaat ttgcgccaaa tttcttctcg
     2341 tcctgtaaat aagtgaactt tgtctgacat tgcagcaaaa taagaaatcg gtaaagctag
     2401 aaccgaaact gctgcataca tccaaaagga caagtgtgta tttcgaaaaa ccttcttagg
     2461 taaaatttct aagataatga agataacaag cgcatataat gctgctttcg attggcaaac
     2521 ccaaatcgtt gccccagcaa gaatcaacgc tggtaatgat agcaatcgca tgtaccattt
     2581 tccaaatgaa ctagcaaatc ctgtgataag aagggctaat gtcattaaag aactacctat
     2641 cgtatttgta ttgatccaaa tatctgataa tttgttcgat ggattccaaa tactttgtcc
     2701 ttctggtact ggtatctcag taaatactcg ataaaccgtt acgagtaaaa agacatagaa
     2761 tactacaaga tacaagctac gatcaattac agtaaatttc gtccatttta caatcaaaat
     2821 aatagcaata attgtcggaa cgatcagatc accatagaaa gtatctcctc ggtagcttct
     2881 aaaaaaagaa aagaaaaggt aaaaaagaat gcctgctgca atgaagaata gatcgtttct
     2941 ctttaccttg tacagattca aaagaaacaa acagcctacg aaaaacgtca ctaactgtgc
     3001 agcattatca taaataaata atatcgtagg aggatttaac cccaccgtcc atattaaata
     3061 tagaatggat accaacagta acagaaaaat ggcactcatt ttattgatca atttttctac
     3121 aagttcttga atgtgactca cttcccttat ttattgattt tttctacatt gactaattca
     3181 cactggatca catcgcactt tttcatatta tttgcttgaa aggctaacag tgtgagtagt
     3241 ggttttttac ctgcttttgg caaggataaa aaggaattgt tttccttaga ttgaactacc
     3301 tggctgtaat cagaaatttt tcctcttttg gagacttcat actgatactt gtaatgagca
     3361 tcgattagat caatttcttc tccttcagat aaatcagtca tatcggataa taaaacgctt
     3421 ggtgtatcta cgttataacc agcaagaata taatttccat ttcccatttc agtatctaga
     3481 taataagtag aagctactcc taaactaagc gtgtcgctat tcgctccttg aaaaacaggg
     3541 agaataaatc ctgtagaaga aaaaacaagt tttccagcaa tttcaccttt gagatccttt
     3601 tctaatgcct gtttttgatt agtaatcagt tgtctcttag aaatatccgt taaactggtt
     3661 aaattatctt gataggtttt agcctgttcc tgaatatgga ttgtttttgt tacaccatct
     3721 actttttcag cgattttgtc cttttgataa ccttgataag caaaataact aaaaagaaga
     3781 aaaacaatga ttacgcccac gtaaggatta aataaagttt tattgttttt attcatctat
     3841 ctaccttcta tatatgaatt aatttaccta catctaacca agataaacca tgttaaattt
     3901 tactattctt tttattcaat aacaatgcca catcataatt gatcattttc atatcataat
     3961 cgtcaagaaa cttctaataa tagttggcaa ttggaacctg tttttacata agttcgcctc
     4021 aactgttcaa agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttatcttc
     4081 atatcattga tatattgcat gacagataat cttttaaggt gtttttgttt catcattttt
     4141 agcaactttt cttccatttc tttcgacact ttttttctgt tttcgatttc ttcataattc
     4201 atgagttttc ctacctttct tgttttttgc aaaaataaaa aagcctatga tcacatacgc
     4261 tctacacttc agtcttgggt tatctttttc tttcgtttaa ttactggccg tgctggtttc
     4321 aaatccttga ttccattatg ggttctccaa taatcgaatt cttttcgtaa tctttctttt
     4381 tccatatctg ttcctttaga cagggatttg aatacatctt taggtgaata accacgagct
     4441 aacagctgta cataccttcc ttttgtcaaa gtatccatat ccatcgcctc taaaactggt
     4501 cgcgccatct aattttcctc ctaaataaat tccttactca atgcctaagg taatactacc
     4561 tttttattat tcgtcgtgac tccttctaag cctttatctg taaaatttat tcctccagtt
     4621 tttattcttc tttatccgct acaaattcaa tgatttcaac gctgacaata taatcactca
     4681 aatatgacca agtatcttca acagataatt cgtctccatt atcatccgta agcgtcattt
     4741 taaaccattc attaccttca tcatcttcag gtagcaagcc gaatccaatt ggattatcag
     4801 tcaatttttg atcgtataaa tcatcctcgt ctaatgctaa aacatctaat cctttacaat
     4861 ttccgcctct tctaaacgtc acaaatcctt tgtaatccca acgttggaaa gaaaccttaa
     4921 tcgtatgtgt gcccaatcgt atattcggat cataattttt catttgttgt aacctcctca
     4981 taatcagtta gtactataaa ggtatctttg tttttatcca aaaaatctaa taaatctttt
     5041 gtagaagata tctcttcatc aacttcacca taataaatac cttcgataat tgttgctata
     5101 tctgaaaaac taatttttac tggacattta tcgtctaccg cattcaatac cgacaaaatt
     5161 gcttcttttt ctgcaatctc tcgtttgaat ttctttggag ctaatcgcat gacactcaag
     5221 tcattttcaa gcttttcaat ccatgcagtt ttactcattt tcgcaaattt ttgaatcatt
     5281 cctctaccac ctttgctatc tactaaatta gttttacttc ataacgaata tctttatcgt
     5341 ttttcgttca ctcaaaaata tataaaataa atcgactcgt ttatggcgtc cgaaacgaga
     5401 aaccctagta gacagcggta aatgctcgat tgatccaatg atcaacgtcc tcgcaagtat
     5461 ctacatgtac tgcagcacac gacacaattt cttgtgcgtc ctccccatta cgagaagacc
     5521 tactccttat tccatgagtt tgcgtcaaag cctagatccc ttagtagctg ctcatgatta
     5581 atctaggcgc tattctaact tgtctaactt ctaaattccc tcttgtttac tctgtgcata
     5641 ttatttacac aaattcattt caccactatc caaacaaggg catttactcg attgaccaat
     5701 tttttacgtt accaatcgta aaaacctacc ccgtcactcc ccgaagggaa ttgttgatag
     5761 atcaagtcta ctccttattt ttctagtgat tctaagttcc tttagaagtc tcgacaatat
     5821 tttcaagcaa gcgaaaaacg ataaaataca agttatgaga atgatcaatt cagctgatta
     5881 aacgccccat cccttttgcg gtacaacgca agactccatt ttttcttcga ccaatcagta
     5941 caccgttttg gtcataaaca tgtttgattg ttggatatag ctcttttaaa gggataggat
     6001 cttttaaaat tatttgtttt tgtttcccaa tgtaataaat catttcttct gagactttgt
     6061 ctttctggat tatatatttc ttacactgca caacggtttt actttgacca gttgatagtt
     6121 tcttcgtgat cttttctgta gcggtacacc gtttaccgta gtgacaaata tcttcgagac
     6181 tcaaaattcc tgtatatgac ataaaataat ccacccactt actttaaata tttttaacaa
     6241 gtttttcttg ttatttttca aaaatagaga attgattttc gttcatgaca aacaaaaaag
     6301 agggaacgca tccaaacatg cgttccctct aatttcagcg ttcgtcatac ggcaaagagc
     6361 c
//
LOCUS       DAFGZE010000120         5932 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.124, whole genome shotgun sequence.
ACCESSION   DAFGZE010000120 DAFGZE010000000
VERSION     DAFGZE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.124"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(158..616)
                     /locus_tag="LTU54_002826"
     CDS             complement(158..616)
                     /locus_tag="LTU54_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8297472.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(629..1960)
                     /locus_tag="LTU54_002827"
     CDS             complement(629..1960)
                     /locus_tag="LTU54_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HBL8297473.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1971..3413)
                     /locus_tag="LTU54_002828"
     CDS             complement(1971..3413)
                     /locus_tag="LTU54_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8297474.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3400..4164)
                     /locus_tag="LTU54_002829"
     CDS             complement(3400..4164)
                     /locus_tag="LTU54_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HBL8297475.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4268..5143)
                     /locus_tag="LTU54_002830"
     CDS             complement(4268..5143)
                     /locus_tag="LTU54_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8297476.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5398..5482)
                     /locus_tag="LTU54_002831"
     tRNA            complement(5398..5482)
                     /locus_tag="LTU54_002831"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5446..5448),aa:Leu,seq:caa)
     gene            complement(5500..5571)
                     /locus_tag="LTU54_002832"
     tRNA            complement(5500..5571)
                     /locus_tag="LTU54_002832"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5537..5539),aa:Gln,seq:ttg)
     gene            complement(5581..5653)
                     /locus_tag="LTU54_002833"
     tRNA            complement(5581..5653)
                     /locus_tag="LTU54_002833"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5618..5620),aa:His,seq:gtg)
     gene            complement(5715..5799)
                     /locus_tag="LTU54_002834"
     tRNA            complement(5715..5799)
                     /locus_tag="LTU54_002834"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5763..5765),aa:Leu,seq:caa)
     gene            complement(5820..5890)
                     /locus_tag="LTU54_002835"
     tRNA            complement(5820..5890)
                     /locus_tag="LTU54_002835"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5856..5858),aa:Cys,seq:gca)
BASE COUNT         1820 a         1260 c          902 g         1950 t
ORIGIN      
        1 cctgaaaata agttttctaa atcataggag caacaaaaaa attcgataat taacaaaaat
       61 caaaatgatt aaatctagtg attgaaacat atcatttcaa aaaaattttg taaattcacc
      121 cataaatatc accctattta acttttttca tcgtacacta aaattcaaaa caactatctt
      181 taatctttac ttcttggttt tgttttaata aatttccttc aaaaatgcct ggagaaacta
      241 caatgatcac cgtatctttt tgtgcttgct ccaacttctt caaatctata atagctaaca
      301 gtgtcccttc ttttacctcc tggttacttt caacccgtat ctgaaacgga cttccttcga
      361 gttcaactgt atctaaaccc atatggatca gtatttttct gccagattca ctttcgatcg
      421 ttatggcatg tcgggtagga aatatattat cgattattcc tttaactggc gcatggatat
      481 atccatcatg attcgttata gcgtatccaa ctcctatcat ttttgaagaa aagacctcgt
      541 cttttactgc tgttaatgga ataatttgac cactagctac agcttttaga atgtttcttg
      601 ttttaaagaa atgcactttt tatcacctct actttaatgc tggccaatat tttttgttta
      661 ctttgatcat ttcatctagt attttttttg cgactctggc atttggtacg gtttgattca
      721 atgtaaatgc ttgtaaagct ttttgataag aaccttcaaa gtaagcatct actagtaact
      781 tctctactgc aacttgtgcc tccatcaatc ctttatgaaa atcactgatt ggttttctta
      841 gtgtaattgg ctccacacca gttgcaccaa catatgccgg tatttcaaca actgcatctg
      901 accgtagatt cggaatcgcc ccttgattag gaacgattag cataaagcga cgacgatcat
      961 cattcaacaa ggaaatagct atatcgacga tatattgtcc atgctcccca aaattaaagt
     1021 tcaatacttc ttccacaata ccgcggcgta ttttatcagc catttctttg gtatttttct
     1081 ctcgtccatc catgaccata ttagcacgtg tatactcttt gtcagtatgc tcaacgacca
     1141 tatcaggata taagtaatac tctaagtaat tattcgggat attacttgga aaatttttcg
     1201 tgatgactga catcatttga aaggtttttt gccaagtttt atctcctata ttaaaatcgg
     1261 cgacttgcat ttcttttgtt attagttttt caataatctc tggcataatt tctcgttgca
     1321 attctttatc ataaatcgat gtataccagc caaagtggtt taaaccataa tacgtaacga
     1381 tccaattctt acgatcataa ccgtaattca ctgcaatcgt ttcttcaatt gaaatagtca
     1441 tatcacaggc attaatcatt tttacattcg gatatttacg acgaacagct tcagaaacga
     1501 tcgactctgg atttgtataa ttcaaaatcc atgctttggg ggcatagtct tgtatatgat
     1561 cgacaatttc caataaacct ttgattgagc gcatcccata agcaaatcct ccgagtccac
     1621 acgtttcttg tcctactaat ccatgtttaa gtggaatctt ttcgtccttt tctcgcatct
     1681 ccaatcctcc aacacgaatt tgtgagaaaa caaaatcgca tcctgtaaaa gcaagctttg
     1741 gatcatccgt tgcacgaatg cgaatatttg aaaaaccttt tcttttcagc ataaatttaa
     1801 caatcaagta catatccata tttctttctt catcaatatc atataaacgt atttcagata
     1861 atggcaaccg tttttcattg ttcaaaatgg cttgaacgat tcctggtgta tacgtactcc
     1921 cgcctccagc tatagtgata atctgttttt tcatcatcat cttttccttt ctattcttct
     1981 aataatttat tgaattctgc cataacataa gtcacttcta agccgatgat aatttgataa
     2041 tttttttcag ttggtttaat gatcccgctt atccctaatt gcttgattcg ctgttcctcg
     2101 accatggtgg gatccactac ctctacccgt aggcgtgtat tacaactata gacatccagg
     2161 atattggctt ttcctccaaa cgcttgtagc atttttgttg ccatatattc ataattttta
     2221 cttgctaacc ctaactctgt ttcttctaag gttacttcat tgccaaactc tgccttagct
     2281 tcacgtccta atgtctgaat gtttctctta ttgatcaaat aataaaaagt aacgtaatac
     2341 aaggcaaaaa agaaaattcc gattggaaga atcaaaatag cattggtagc gattcgaaaa
     2401 ttgatgagat aatcaacaat acatgctccg aatgtaaaac caattcgtat attgaataag
     2461 taacagacaa tccctgctaa accggcaaaa agtgcatgga tcacgtacaa cataggagaa
     2521 agaaacatga aactaaattc aacaggctca gtgatatttg caataaatga agttcctgca
     2581 ccgcttccca tgattccttt gaccatctct ttattttctt tataagctgc tttatagata
     2641 gctagacata tagctggtat accaaacatc attactacaa aaaaaactga taaaaacagt
     2701 ccagcctgag gatctccgtt gataaagcgt gtcatctctc ctctgaaaac ttccttgcta
     2761 ggagatgtat agcttcctaa atcgtaataa atatacgtat tcaacacatg gtgcagtcca
     2821 aaggggatca gcaaacgatt caaaaacata aatatcccca cacctaatgc tcccatatca
     2881 acgagcagtt ttgcaaaatt atcaatccca atttggatga gcggccagat aaagcccaaa
     2941 aaaacagctg cgattgtttg tagaatcatc accatagtca atgggaattt ttcgcccgca
     3001 aaataagaaa aaactactgg taatttttgt tctttaaagc gattatacgt ccatgccgct
     3061 actaaaccgg aaataattcc accaaaaatc cccatattca cttctgggtc cataatttct
     3121 aagccttttt tcaaggtagc aatcgctaaa aaaccagtaa gtgcaggtat tcctttgtcc
     3181 ttatttttga caaaaccgat tgcaactcct atagcaaata agatatccaa gtttccaaaa
     3241 acggcatttc cagctgcacc aattactggg atgttcagca tatcgtcagc tgataaacgc
     3301 atgatcaatc ccgctaatgg catcgctgca actggaagta gcatcgctct tccaagcttc
     3361 tgcacatttg atttaacatt catttttatt cctcctctat taaatatcct catatgttac
     3421 taactcaatc aaaggatcat ttttcacttt ttgagcaaat gctgaatgaa ttaaaaattc
     3481 gacttggtgg gtacgaggat acacaaaact tgaaccattt aacaattcct gatccaaata
     3541 cgctgcgtga cacatgactt cgatcgtttt atcttctctc aatatcatga gtaaacttgc
     3601 caaatattct tccacactcg tattgatttg tcgttttttt tcatcagttt ctcctacgac
     3661 atcgaaacaa ggctcaaaat aatcgacatg acgaacttca tcttttcgtt caaagttgct
     3721 ccgaaccggc aaatcatatt tttttgctaa tgtaacaact acctcctgat tttcatccaa
     3781 agtatgagta tgatgatgac tgtctaaatg agttggtatg atccccgcat gataaatttt
     3841 ttgaatctga gcattccatt cctgatacag ttgatctttg tcaatgttaa atggatgctg
     3901 ataataagat aaggatctga actctccgct ttcgataaga gtatcgacag ttttgaggag
     3961 tggtttgcca cacgtcaatg ttaaatggac tcctacacct agcctaggca tttcctttct
     4021 tagtttcaca gcatgctcga aacccggcat atttgccatc aatgtagttg atgtcaaaat
     4081 tcctcgctga taagcatcca taatcccata attcacacca tggctatagc caaaatcatc
     4141 cgaattaaaa atcacttttc ccatttcatt ctccttcttt tttctactaa aataggcgca
     4201 accaaataat catagtccat ttactatgtg tcatttggtt gcgcctatag atagttacgc
     4261 ccttatttta atagattctt gctagatgaa ttgccaaata gatgatttca ttttcttgaa
     4321 tggcaagttg aaattgctgt tcgatgatat ctttaatctt tagtgctgta caatacgctt
     4381 tgctatgttt aaatgtttca gtaaaaaaac caatattttc tactggcgtt tcttcctgat
     4441 aaatcaagcg tttaaccagg aatttgatat gaaccattaa gcgatgataa gtcagtgatt
     4501 ctttatcata aacgtttgaa taggattttt ctagatggga caaaatcgtg gtcgtcattt
     4561 ctactatttt taagctcttt aaactattag gctctcctag ctcagcatta atgatatgca
     4621 tagtgatgaa tcctacttca tttgacccta actcggtttg atatcgatga ttgattcgct
     4681 cgaccacttc tactgctata tcatattcat ttggataaag gaatctcatt tctggcaaca
     4741 taattgctgt gacatctcta tcttctcttt ttcgttgaat agcaaaataa atatgatctg
     4801 tcaatgtaat atagatattt gaatctaatg gagaggagat cctagctgtt atcagttcaa
     4861 tcacttcttc cgcaaaatcc ataaattcga ttggaatctg cgagactatc tcactgaatc
     4921 gtttgatctt ttctttagaa tctaatacaa ataatttgtc agcatcctca acatcaaatg
     4981 gcttactcac tttagcagaa aagccaatcc ctttccccat cgcaattttt tctattccat
     5041 cgtcatcgat caatacagca ttatgattca gctttttcat cacatacacc gttaattcac
     5101 ctcctctata tgataaaaca aaacaacttg cagactcctt catacatatt tttgtctcca
     5161 aacaattttt aaagacaaaa aacgacctaa gaaagaaccc tttcttaggt cgtgcctaat
     5221 tgaatagtag caccctacat aatttacaaa aatagtttaa cacataataa aagcgttatc
     5281 aatatctttt ttactcgttt tatgataata cttgtatcga attgatttgg aggtaattac
     5341 ataaaaacaa aaaaaatata gcaactctct ataagaaagt tgctatattg ataattatac
     5401 cggcggccgg ggtcgaaccg gcacgtcctc aacggacact ggattttgag tccagcgcgt
     5461 ctgccaattc cgccacgccg gcaaatttaa aaataataac tggggtagct ggattcgaac
     5521 caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc aagaataaag
     5581 ggcgaatgat gggaatcgaa cccacgaatg ccggagccac aatccggtgc gttaaccact
     5641 tcgccacatt cgccatcata aaagatgtta tttaatttct caataaaaaa ctaagatgaa
     5701 gacatcttag tcaataccgg cggccggggt cgaaccggca cgccctcaac gggcactgga
     5761 ttttgagtcc agcgcgtctg ccaattccgc cacgccggca agttgattta ttagtactaa
     5821 ggcggtaacc ggatttgaac cggtgatgaa ggttttgcag acctctgcct taccacttgg
     5881 ctataccgcc attatactat tatcaatcta taaaataaag aataaattat ag
//
LOCUS       DAFGZE010000121         5874 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.125, whole genome shotgun sequence.
ACCESSION   DAFGZE010000121 DAFGZE010000000
VERSION     DAFGZE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.125"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..3143)
                     /locus_tag="LTU54_002836"
     CDS             complement(<1..3143)
                     /locus_tag="LTU54_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBL8297477.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENE"
     gene            complement(3157..4374)
                     /locus_tag="LTU54_002837"
     CDS             complement(3157..4374)
                     /locus_tag="LTU54_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBL8297478.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4364..>5874)
                     /locus_tag="LTU54_002838"
     CDS             complement(4364..>5874)
                     /locus_tag="LTU54_002838"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBL8297479.1"
                     /translation="LGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQTMLYRELLSD
                     EESKEDLIATIVDILGYSISPEYLFNVLADQAKQATFQLNDLNKAFVQLASTYNQFNG
                     LFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVEVLGHDGDVIGDAYEFLISQFASEA
                     GKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVK
                     YHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYS
                     ANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGA
                     AEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVK
                     GKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEP
                     IDMVHVGNDIKKIRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT         1600 a         1248 c          990 g         2036 t
ORIGIN      
        1 aattcatttt caagtggaat caactcatca tctaacattt tacgccatgc tcgcttcatt
       61 tcttgtgggt atttaaatgg tttcgcttct ggatgtttgg ctttatatcc ttcgtaatcc
      121 ttactgttga taattgcttt catattagga attggatctt cacctggcat atattgcaga
      181 ttagaagcat gtaattcttt ctcagaaaca aaccactcat tggaaaaggc atgaatcaaa
      241 ttatcacttg cttgtgtaaa atagcgacgt ttaatgacaa agatatcttc attactatct
      301 aaagtcccca catcaaatgc atttaaaatt tgtcggtaaa cttctttgac tccttctgtt
      361 ttattcaatt tagccaaagc tttttcaatt tcttctcgtg catctgaact attcgcagca
      421 taagttccta gtaattgatc aatataagta gaatcaatat catacaactt agtggtattt
      481 tcactataaa attcgatcgt tgaaaaatct tctccctctt tttctggttc ttcctccatc
      541 aatgaaccct tcacggtctc gtacacgcca atttgttctt ccaaaagttg aatttgattg
      601 ttcaaagttg aagattgcac catgtcatca ttgtaatcat cataagtcac taacgattca
      661 tacgcacgat tcaattcttg aaatgctttg acgtactcga tacggatttc aagtggcgta
      721 tgttcatcaa tggattccat ggatgaaacc tcttttaact taccatatgc ttgcttgaat
      781 ctttttttgg attcttggta acttggataa atcaaacccg attcttcttt agcttctgaa
      841 tacagttttg tcgcatcctc gacattcttc tccattgtcg ctggctttcg gaaagtaaca
      901 attagtcctt ttgttttttc aggatacgta cggttcgtac gtgaaaaggc ttggatcaat
      961 cctgcatact ctagattacg atccacaaat agggtttgaa tcgtaggggc gtcaaaacct
     1021 gtcaatagac gatccactac gattactaaa tcgatttgtt tcccaaattg tttgaattcc
     1081 gctctttttc gtgccaaacg attattgatg tctccgttat aacgctcaat gtctgctagt
     1141 gaccaggcag tatcataata ctgattatat tcctcaataa ttttttgcat ttcttcttgt
     1201 tgggcacttg aatctcggct attttcttct aatgaatagg taatagcgat tcgtgggaaa
     1261 tcaggatcct ccatcgttcg tccttcacgg ataggctgat tgggaaactc ttttattaac
     1321 caatcagatt ctttcgtcat ctctttgatg gcccgataat actttttggc catatcaatg
     1381 gaactcgtgg ttaagatcgc agacttcgtt ggacggccat ttcgaaaatc aaattttgta
     1441 taggcattgt ctggacggaa cattttatga atgacttttt ggatatgttc ctctgcttca
     1501 aaaacagtag ggtccaaata actttctttt ttgacagact ccatttgatc gatcatacga
     1561 ttgatctgtt ccggtgaata ctctgcatat gtttctactt cttttaattt ccgatagatt
     1621 ttattctcta atgaagtcgg ctccacggtg ttttcatgct ctacttgaaa tcctaaaacc
     1681 gagccatctt ctaatgcatt cttgatggta taggtgtgca agacctctcc atactgatca
     1741 tgggtcgtac gtgccaattg ccctttggct tgtttccgat tctcagggaa aatcggtgta
     1801 cctgtaaagc caaaccaagt ggatttcgga aacatttttt tgatttcttc catattttca
     1861 gcacttaacg ctcgatgaca ctcatcaact acaaacacga tatgttgccc cattaatttt
     1921 tggaagcgat tggttccttt tttctcttcc tgtttctttg catatttcaa cgccgcatct
     1981 agtttttgac gggttgtaat gatcacaaca ttcgcattgg catctgccaa cagagtttca
     2041 cttaactctt tggcacttcc tgttcccacg atcaagctat tcgctttggc attaccagaa
     2101 gaaaccccag tattaaactc cgaagcaaat ttcgtaaact ctgtggtggt ctgattatct
     2161 aaatctttgc ggtccactaa catgatcgtt cgatcgatcc ccgattttcg tgctaaaagc
     2221 ttagtagata caaaactagt caacgtcttt cctgaaccag tcgcatgcca cacataccct
     2281 gattgatgtt tatttgcggc aataaacaag gcttgaatcg catgcacttg ataaggatgt
     2341 aacaccatga gggttttatt gtcttgatct tcactgacaa tcgtataatc cgcaatcaag
     2401 cgatgggcat ctgggatatt tagcacttgt ttgcaaaact catataagtt ttctaccttt
     2461 ttattatctt tcgttcgcca actaaataaa aacttaggat gcaaatcctt cggtaaggca
     2521 tttgcaaaat accgagtcgt ttgttcattg gaaaccacaa acaactgtaa ggtcgaaaag
     2581 atattgttcc gaaacatgcc ttcttcagca tattttttga tttgattaaa cgcctgataa
     2641 aaaccatctt ttgcagttac ttgttttagc tcgatttgga taatcggtaa gccattgatc
     2701 aataaggtga cgtcaaaacg tcgatcccgc gcttctatgt tacttccacg ttttgcaatt
     2761 tgatggacca cttcataagt agagattcct ccaccaatgt cttgattgga ataaaggata
     2821 agtgagacag aacccaattg cggattttcc cgttcaatcg tgatccgagc catcccattt
     2881 tcacctttta accatttcgc agcatcaaat ggggttttcg tccgtagcaa taattcggtt
     2941 ttgatcgttt caaactcttt atcggtcaaa ggagaatcat tcagttctgc ttgattattt
     3001 gagatgattt tttgacgtaa attgctccaa agatcttctt ctgattttag atccggacga
     3061 tacgtccact gattatgtcc ttccccgagg acacgaatca actgttcttc gacttccgct
     3121 tcctcacggt gactaatttt cattcttctt ccctccctaa acaaacatct tttgcaagaa
     3181 gccttttttc gtttccttca gcaaatctaa cttacgttga tgaagagcga tcgtatcatc
     3241 tagctgcttg aagaaggcac caattttttg ttgttcattt atatccggtg gaaactgaaa
     3301 tgtcctttgt tctataccac ctttagtcac atgtaccatt gtcgttccgt tagttgattc
     3361 tttaattttc tctttatcag tcaatagcca agtatataga tactgtttgt ccaattcctt
     3421 agataaaaat ttaagtttcc aaatatgata atggtagatt acacgttctt cattccacat
     3481 ttcaggaccg aagcttgttg cccataaata taagagatct cctttattag catacttgtc
     3541 ttcctctaat tctaagtttg aataatacca tctatcatta gtattaaaat tacctactct
     3601 taaaacccta tattttccag aatcaagtaa ttcttgttgt ttaaaagctc taccattcaa
     3661 aaaaactacc acttccccca acttccgctc ttcccaatct cccgtaaatc ctggaaaacg
     3721 aacttctggc acttttgctc cgtttttagg gaacattttt tgtaagaatc cttttttcgt
     3781 ttccttcagc aaatctaatt cgtgctggtg aagcatgatg gtgtgatcaa gttgtttgaa
     3841 aaatgaaccg atcttgatct gttcgtcata agttggaagt gagataggtg tctttttacc
     3901 actatcaatt gtataggttc cgacggttcc aatgcctgca acgtcacgga tatacttctg
     3961 aattttgtta tttatgaacg agtagtaaaa gaagtttccg tcaattttat gctcattctt
     4021 gaaccaaatg atgtttccat ccttaaagta aattgggtta tcatcagtga ttaataaggg
     4081 gacacctatt gatccgacac cagttacaag caagtctcct tgagaaactt tacctgaatt
     4141 tttcgaatat tcattatatt tttcttctga aatgtaaaga taatcggatg gttcctcatt
     4201 ttttgcagct gagacaatat cacgagcacg taaaaatcta atacctgagt tagtccaatc
     4261 tgattgatga attcgcttca cagaagtaac atccatcata tctcctaact tacgctcttc
     4321 ccaatcttcc gtaaaaccag gaaaccggat ttctggtcgt gtatcattgc tcatgtttga
     4381 acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc agggaagaga
     4441 tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc ttgatgtcat
     4501 ttccgacatg aaccatatca attggttctt cttcttcgaa agtatcgaca tagcgtggga
     4561 tatttaagtt atagtcattt tctttgattt catcaaaggt tgccaaatga gcgtatttct
     4621 cgacatcttt tctttcagcg taggtttcga ggattttttg aatgttttct tccgataatt
     4681 tattctgatt tttcccttta acaaattcac gactagcatc aatgaataat acatctcgag
     4741 tttgacgatt cttcttcaat acaatcaccg tcgttggaat cgatgtacca aagaacaagt
     4801 tcgcaggcat accgatcaca gcataaatac tgccgtcttc taataatttt tgacgaatga
     4861 ccccttctgc agcaccacgg aataaaaccc cgtgtggcaa gacgatagcc atagtccctg
     4921 tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg gattttggcg
     4981 ctaatttccc gtagcgatta aaccgagaat catctaagaa ggtcgtatct gctgaccaat
     5041 tagccgaata aggtggattc atcactactg catcaaatgt gtaaggttca tctgttggcc
     5101 agtccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct accccatgta
     5161 aaatcaagtt catttttgct agattgtatg tcgttgtatt tagttcttgc ccatgatatt
     5221 taacgttatc tggatgagtt aagtagttac gtacatttaa catcaaggaa cctgaaccca
     5281 tggttgggtc aaagacacta aagaatcgtc gttctttttg atccaacgtc acgatttgtg
     5341 ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc cctgcttctg
     5401 aagcaaattg actgatcaga aattcatacg catccccgat cacatcccca tcgtgaccta
     5461 aaacttccac gtcattcaat ttcttgataa cttcggtaat cgtcacattt cgttgttgct
     5521 catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca ttaaattgat
     5581 tgtaggtaga tgccaattgc acaaaggctt tgtttagatc gttcaattgg aacgttgctt
     5641 gttttgcttg atccgctaag acgttgaaaa gatactctgg tgagatcgaa taccccaaaa
     5701 tatcaacgat tgtggcaatc aggtcttctt ttgattcttc atctgaaagt aattcacgat
     5761 acaacattgt ttgtttgctt actgtgtcat attcctctaa ggattcatct gctaaaagaa
     5821 ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc aata
//
LOCUS       DAFGZE010000122         5749 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.126, whole genome shotgun sequence.
ACCESSION   DAFGZE010000122 DAFGZE010000000
VERSION     DAFGZE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.126"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..435
                     /locus_tag="LTU54_002839"
     CDS             <1..435
                     /locus_tag="LTU54_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBL8297480.1"
                     /translation="EKDYWGESKGFIYPDSNMGGNWKATNPKKEQEAMKLKNTKSNNL
                     LYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSGGQNISSVSYETAL
                     LEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            455..1015
                     /locus_tag="LTU54_002840"
     CDS             455..1015
                     /locus_tag="LTU54_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HBL8297481.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGILSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADE
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            1839..2606
                     /locus_tag="LTU54_002841"
     CDS             1839..2606
                     /locus_tag="LTU54_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBL8297482.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIREKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            2632..3174
                     /locus_tag="LTU54_002842"
     CDS             2632..3174
                     /locus_tag="LTU54_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297483.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFATYESGMSAYDETGFDGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGYSLDDKTRLRLFDIEGKQIGLNKFCKNGKEFATKDF
                     IKGYRLLLENYGINLEESVK"
     gene            3175..3741
                     /locus_tag="LTU54_002843"
     CDS             3175..3741
                     /locus_tag="LTU54_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297484.1"
                     /translation="MFQKRKDKFPNLEVKSQKVIDGEMIELFQKNYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            3756..4004
                     /locus_tag="LTU54_002844"
     CDS             3756..4004
                     /locus_tag="LTU54_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297485.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTENLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            4024..4263
                     /locus_tag="LTU54_002845"
     CDS             4024..4263
                     /locus_tag="LTU54_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297486.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            4634..4915
                     /locus_tag="LTU54_002846"
     CDS             4634..4915
                     /locus_tag="LTU54_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBL8297487.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            4905..5246
                     /locus_tag="LTU54_002847"
     CDS             4905..5246
                     /locus_tag="LTU54_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL8297488.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIGEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NKFIERLQRKV"
BASE COUNT         2169 a          766 c         1162 g         1652 t
ORIGIN      
        1 gaaaaagatt attggggtga gtcaaaaggg tttatttatc cagattcaaa catgggaggg
       61 aactggaaag ctacaaatcc caaaaaagag caagaagcca tgaaattaaa gaatacaaaa
      121 agtaataatt tattatatgc tacgtgtaaa cattttcgac atgtacgaga tacagaattt
      181 acgagttatc atctttcagg aatagtaatt gacagtttcg tatatgaggc tatgggaaat
      241 tggaaatttg ttgaaaataa cagtgggggt caaaacatta gttctgtatc ttatgagaca
      301 gctttattag aatactataa ctcacataaa gttatgggcg gtttaaattt atactctcct
      361 ggaagcaatc aatttgttaa ttctgatagt agtatcatat gtttagaaaa agtattaaaa
      421 aaaatagctt tataaagttt tatggaggaa caacatggga gacgaaaaaa agcgcttgga
      481 agttcaatta cgtgaagcat atggacgagt aacgtatact tacaccactc atttaaagca
      541 agccgataat attatttctg acaataaaat tttaaggtgt cttcaaattg gtttgtcagc
      601 ccttactact ggagggatac ttagtaccat tattactgat aatagtgagt taacctttgt
      661 tgctggatta tgttcaacaa tttctcttgg attaaactta tactttaaga actttgatct
      721 tgctgaagaa gctaaaaatc atcaaattgt tgccgatgaa ttatggttga tcagagaaga
      781 atacgtaagc ttactaacag atttaaatcg tttaagtgtt gaagatattg tgaaaaaaag
      841 agatcaatta atgaacgaaa ctcatgaagc atataaaaat gcccctaaaa caaatagtaa
      901 aagctatgaa tcagctcaga aagcattaaa atctgaagaa gagcaatttt ttacagaaga
      961 ggaattggat aagatgttac cagagcatct tagaaaaaag agttctaaat attgaactct
     1021 ttttttatgc ttaaaattct cttgtaattt ttctattcaa tcaataaata atctatattt
     1081 aagaagaaat atgatgatag aaacatctcc taaagccacc gcaagccgtt ctaagcatag
     1141 cttagcccga cattgctaga tatgctcatc tcaccatgaa cgtttcctaa agccaccgca
     1201 agccgtccta gtcattgcct agtccgacat cgctagatta gtgcatctcg ccataaaagt
     1261 tttctaaagc caccgcaaac cgtcctagcc atcgcctagt ccgattttgc ttgatcggtt
     1321 ccatttctgt ttatttagtg ggtcaacacc gtaatcttaa atagccatcg cctatttaag
     1381 attactaggt ctgctttgaa ggtaaaaagt ctttcattct tcaagacttt taggctttca
     1441 agctacgcct gaaagcattg aaattcatcg ctctttattt ggttttgcca tttataattc
     1501 cttacgccca ttatcttttt cgcctgtctt gccttgtcag aagtgtttag agcttatctc
     1561 atctttggat taatacaagt ggcgtaaatt tttttctaaa cgcaagcgtt acgaaaataa
     1621 tttacttaaa cacttgtatt aatccggcag ataagaacgc attctaatca ctcccgacaa
     1681 ggcaagacag gcgaaaaaga taaataaatg aacgaaagga agaaacataa aatgcaaaac
     1741 caaatagcta tgaagaaaaa aacacctccc tctaaaacaa tccaaacaaa aaagaatttt
     1801 gaaaaaccaa aaatcaaaaa cagaaagaag cgttcaacat gagagataga attttagaag
     1861 ttaatcaaga aatggtaaac aagttaacaa atatcagtgg gaactatgaa aagtacgatg
     1921 gtactttact tgataactat atttttcacg atacacaaaa tatcagaatt ggcagaagta
     1981 aaccgagagc gttcattctc atcagagaaa aatatttgaa tgaatggaat agcacctatg
     2041 aaatggttat gactgataac tttgaaaagg tattggattt cagaaatgct ttgttgggtt
     2101 ctgatatggg aaatagcagt cttgaatcta ttgaagttga aaacaagacc gagaatgaag
     2161 gtgaaggatt gattaagaat gtcttgaaaa tcgaacaaaa atatatcagt gaagatagaa
     2221 gtttttatag agtcacaagc tcggttggtc tagcaaactt tgctactaac ttaattggaa
     2281 acaatgctag tgaatctttg ctagtgatgt gtctgaatac aaagaatgag gtcattgctt
     2341 atagcgaagt tttcaaaggt ggaattaatc aatcagtagc gataccaaga gagatttttc
     2401 aactagcttt gctaaacaat tcagcaaaaa tcgcagtatc acataatcat ccgagcggat
     2461 atgaatttcc aagtgaaaat gacaaaagtt ttactacgaa tttgaaaaac gcaggaaaaa
     2521 caattggtat tgaattgtta gaccatatca tcgtaacaag taaaaacaca agctattact
     2581 catttcaaga aaatggttct ttataaaaat tagagaaaag aggaaaacaa gatgggcgaa
     2641 agaacacaag cgttaattac agtcaaatat ggtaacagca aaacgaaagc aagcgtgtcg
     2701 attcattatc agtgggggta tggtcgatat atgctgatgg acgtattgaa tcaaggaatt
     2761 tatttaatca ataaagcata cgacttacga gataaatcaa gagaagaatt agcaaaagga
     2821 atctttgata gaagtacagg ggttatcaat tttgccacct atgaatcagg tatgagtgct
     2881 tatgatgaaa caggatttga tgggcgctat gaaccttatc aagaaacgat tgaagatgtg
     2941 atgaagtttt ctgataacaa tgatggctat gctcatttag agatggtgtt cgatgaaaat
     3001 ggatatagtc tagatgataa aaccagattg cgattgtttg atattgaggg taaacagata
     3061 ggactaaaca agttttgtaa gaatggaaaa gagtttgcta caaaagattt tattaaggga
     3121 tacagattgt tgctagaaaa ttatggaata aacctagaag agagcgtgaa ataaatgttt
     3181 caaaaaagaa aagacaaatt ccctaattta gaagtgaaaa gtcaaaaagt gattgatggt
     3241 gaaatgattg aactattcca aaaaaattat ggaacgatag aaactaccgc aagttgcaaa
     3301 tactactttc aaggaaagag aaacacgttt aaaggctttg ccgattctga tgaagaattg
     3361 gcaaagaaaa tcattgattg gcttgtcagc agtgaacacg tagcaagaga agataccgtt
     3421 agaacaacga ttaatgggga agtcctaaca ggtactagat gtgtttgttg taatcatgaa
     3481 tttgtagaag atgaaggttg ctaccgaatc aatgaatatg gtgattttat ttgccagagt
     3541 tgccacgaga tagaatttcc aaccgaaaga agttggtttg aatacatgct acaagattat
     3601 gagatggaaa aagaaaatgg tgaagtagtt ttttgtacca aagaggaatt gagtaagaga
     3661 agcgatgatg aaattgaaga ttggtgtgtg tggttagtag gggcagatga ttgcgttgca
     3721 tattacacaa cagcagaata ggagtgtgaa tcagcatgga aggaatggat tttctagatc
     3781 atgaagattt ggttgatttt ggttatacat ggaaaggaat ggtaggtatt agcagaagtc
     3841 ttgctaatgc cttttacgaa agaaattatg cagtatatgt tctttatgat gacgatacag
     3901 aaaaccttgt tgacgaggaa tataaattgg atttagagaa tgttttatat ggaatagaaa
     3961 aggaagattt agcaaaatat attttttcat ggttgggtca ataagtgaaa ggaagataga
     4021 aacatgacaa aatttaaaag taagggtatg caatggtgtt cttattatta tgttggaaat
     4081 gatgaagatt tattgagaaa aacccaacga aaaggttatc cagataagat attgaaacaa
     4141 ataggttatt acatgccaga agagaggata gaaaaagaat tttgtgaggt tctttttgat
     4201 agtgaacagc tattagtttt gttaacccaa aataaattga tttgccaatt tgaaaataat
     4261 taagatgagg tggtgaacca tgaatgaaga taagatggta ttaagtgaat atgctagatt
     4321 gagaactatt aattctttag catctcattt aagttgttct ttccttacaa aggtgctaaa
     4381 attaatttac aaatgtgaag attttgtaca agagaagcac aaagacaagc ctactgcgaa
     4441 taggcttgtc tttttttcat tcattacgat tccgtatgtg tgggttagcg acaatttgtt
     4501 caattaccgt atgatatagc atcttaattt gattagatta cttgcaatgt agtttattaa
     4561 gagtataata taatcataaa tgtggataat ttgagaaata atgagtgttt tatactcaaa
     4621 aggagtggat gaaatgaagg tcaaaaatcc aactgaaaaa agcagaatcc aagtcggaat
     4681 agacaagaat ttaaaagaga acgcagaaat gatattggaa gagctaggtt taaatcctac
     4741 aacagcaata acaattcttt acaaacaagt agtcgctaga ggtgagtttc cagtagagat
     4801 aaagctaagt gaagaggaga agcaaggaat cagattgcaa cagctaacta aggacatgcc
     4861 agtagatgtt ctagatacag atgaaaagtt agaagagtgg ttcgatgaag cctaatgaga
     4921 tagtaacggt ctatgttgca tttacagata aaaatggtgg taaaagaaga cctattttgg
     4981 tggttagtga taaagaagat agagtagaat tttttggaat cacaagtcaa tatgagaaga
     5041 aatcagacca aataaagaga gtgtattttc ctattggaga atgggaaaaa gcaggtctta
     5101 aaaagcaatc atggattgac gtagggtctt taaaggctat tcccaaaagc cgagagaaca
     5161 tatcatttaa aaaggttggt atgctatcag ctagtgatac gaaaaagcta aataagttta
     5221 tcgagagatt acaaagaaaa gtctaacaag gcttttcttt ttttgcttat atgtatatta
     5281 agatatacgt ctatacaaac gtatttatgt atataaaaat acacgtaaat atatacgtga
     5341 atataaacat taagaaaata ttaagaatga ttaaatagtg gtaattgtta atatacgaat
     5401 tataagatat attttaaatc gaatgtatgt aaataaataa aatgactgga ggatatttaa
     5461 tagtgataaa taacagtaat ccagacggat acagtgagtg aagtagcttg ctacgaacga
     5521 acaagcagaa tgaaacggta gtttcattag agcaagtcgc ttgcgacgta gcgttaccgc
     5581 tgatgtaact ggcaagttac atagagagag tttgcttgca aacgaacgtt acctacttga
     5641 atagaaaaca aaaataacag attagtgaat tttaaatagg agccacaact caaccaaccg
     5701 cttgcggtgg gtgagtccca cggaggaaaa gacgaacgaa cggtcgcgt
//
LOCUS       DAFGZE010000123         5689 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.127, whole genome shotgun sequence.
ACCESSION   DAFGZE010000123 DAFGZE010000000
VERSION     DAFGZE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.127"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(488..1297)
                     /locus_tag="LTU54_002848"
     CDS             complement(488..1297)
                     /locus_tag="LTU54_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8297489.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1297..2043)
                     /locus_tag="LTU54_002849"
     CDS             complement(1297..2043)
                     /locus_tag="LTU54_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297490.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2061..2528)
                     /locus_tag="LTU54_002850"
     CDS             complement(2061..2528)
                     /locus_tag="LTU54_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297491.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2528..2938)
                     /locus_tag="LTU54_002851"
     CDS             complement(2528..2938)
                     /locus_tag="LTU54_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL8297492.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2949..3962)
                     /locus_tag="LTU54_002852"
     CDS             complement(2949..3962)
                     /locus_tag="LTU54_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8297493.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4179..4913
                     /locus_tag="LTU54_002853"
     CDS             4179..4913
                     /locus_tag="LTU54_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8297494.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4897..5607
                     /locus_tag="LTU54_002854"
     CDS             4897..5607
                     /locus_tag="LTU54_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8297495.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSGSVAKF"
BASE COUNT         1832 a         1195 c          943 g         1719 t
ORIGIN      
        1 aagcgacaaa aaccacgagc aggtccattc atcctcaaac ctcggtgatc ttcgcggttt
       61 aacaccatca gaagaaatgc acaaaaataa taaggagagt ctgagacata aataaattcc
      121 cagactttta gtgagtctaa atccgaataa acggtgggtc gaaagcaact ctagctgcgc
      181 gtcgatcgca gcaatctcta ggtgctatgt tcggataaat ggtggtcaaa agtcagattt
      241 tcggccattt cgtaacagcc aaccagacaa aaagcgtctg ttttgtcttc tactccaatt
      301 gctcaaatct tagcgacttt tggcttggtc ttactctaaa cagtttgtaa gcctaaactt
      361 tatttgctgt ttgtttctaa gatcaaagca aaactacttt ttagttggtt acttttacaa
      421 gttcttttta ctgttgaata aatgtaaatc ttaagaacaa atatttactt ctataattcc
      481 aagtttctta aaaaattccg ccaagtgtac ctaaaaagca taaaacaaaa attcctagaa
      541 taattgtgat cgttttatgc cctttggagt aaagccagac gcataaaaag gtaattccta
      601 atggcagcaa gcccggaaaa atttgatcaa aaatactttg taatgaagtt tcagctccac
      661 caaccgtgaa ttttagtgac gtatttaaag taaccatcgt gcctatcatc gcgccaatta
      721 ccgttaaacc taaaatcgaa gcaccatgag ataatttgtc aatcatatgg ttgtcttctg
      781 tatcatcaat aaatttagtt cctaaaacat agccatattt tgtcgccaac catttaaaaa
      841 taaaatgaat ggcagtatac aaagtaaaat aaaggatcat gcctaaaata ttcccttgtt
      901 gagcaaaaga aatacctatg ccagccgcaa taacccgaaa agttccccag aaaaatgaat
      961 cgccaatacc tgataaaggt cccattaatc ctgttttgat tttattaatt gcgtctgtat
     1021 caaattcagg atttttagca ttttcttctt ccatggtgac ggacaagctc ataacaaaag
     1081 gcgcaatatg cggtgtaatg ttaaatgttt ccgtatggcg ctgcaacgct gtataataat
     1141 catcatcgtt cggatatatt ttacgtaagg aacgttcaat accattcatt tgtccatatg
     1201 ccatcatttt atcgaaggac caagaggatt gcataaaaaa ggagcgccaa aatatacttc
     1261 ttaattcttt tttcccaata acactttctt ttaccattat aaatcttcct cctcttcaaa
     1321 agttagcttt tgaacagcca gtaacgctaa gcaaagtccg acgacagcca caccgacaac
     1381 atccattttc agatacatta cgccgacaaa tcctaagaaa agataaggca tcatcgcttt
     1441 attcgccatc attttaatta ataacgctag cccaacacaa gaaagcacac cggccgcaac
     1501 atcaaagctg tccaatacaa cctgaggaat caacttgatc atcgcattga ttgtagacac
     1561 accaaaataa gtagctacaa aaactaaaat ggccgatgga accccaacga ttaaaggttg
     1621 agccacataa tgatattttt gtgctttttt gaaatcatga ttgactaatg cctcatccat
     1681 tttgttgtta aaatacgtaa ttggcattgc ataggcgaag ttacgccaca tctgaagaaa
     1741 gatggacaaa ggtaaagtca acgcaacggc agttcctact ccttgacccg attgaatcgc
     1801 aattgctgta cctaaaacac tggaagcata aacttcaggg ggcgtcgcac tccctaccat
     1861 aattgagccc atgaacatgg attccaaggt ggcaccaata ataattcctg actctaaatc
     1921 tcccaaaatc aaaccgacaa aaggcgctgt aaatagcggt ctacccatca tcgatgcacc
     1981 aaatgtgtat tcatcaatag acgccaaaaa agctaccagt gcgattaata ttgcttgtat
     2041 catattcttc ccctcctctt ttagtcatgc aaaggattga tttttttgtc cgttggtaca
     2101 gcctgacatt gaactgttat cccgttttca gtcatttttt tcaccaagtc cagttcttgt
     2161 tcattcaagt aaacggaact agtaatcatt ttccgattct cgtttttctt taaatttcct
     2221 aaattcactt ctttaatctc aggtacgtgc tgagtaattt cgtaggcatc ctccaccgtc
     2281 ccgacaatta aaaatacttg gtatttcttg attttttctc catttaaaac agagatggcc
     2341 tctgtcatat ccttcaccac aaattttgtc cctgtaggaa cagccatttt tagtgttttt
     2401 ttgcgcagat tattggtagc gatttcatta cttgcaacga aaatacaatt tgcacctaag
     2461 ttatttgtcc aggcaaaagc aacttgccca tgaacaaaac gttcatctac tcgtaataat
     2521 ttaatcatta aaaatcatcc tcttctgttt tctctagcaa ctcgttcacc aaaactatcc
     2581 cttcattccc gctattcata gctttttgga ttagtttttt cgtgtcctgt tcacctgata
     2641 agcaaagctc taaaagcagc ggcagattta caccgctgat taaataaaaa ttgtctttac
     2701 cgatttgttc ggagaaagcg ttagacacgc ttcctcccag taaatctacc gtcacaatta
     2761 attcgtcatc aggcgctaag tcggaaaata gctgctctat ttcctggtta atggagtagt
     2821 cactatctat atacgcagtt attgctaaaa gtgatttttg aggaccaata ataaattcta
     2881 aagtatcctt taagccttta gccatcatgg aatgcgtcgc aattacaatt ttcctcatag
     2941 ctcttacctt aatattccac agtccgataa taacgacgaa tatctagtga atgatcccgc
     3001 accttttcaa aatgagcact cactcgttct aaggccgttg ccataaccat tggcgctaca
     3061 tactgacgca tagcgcttgg tacacctgct aattcgaaat ccgcagtgtc aaaaaccgtc
     3121 acaacatcgc tatatttatt tacaaaacgc tctacgcgat cgactagcgg acgcgtttga
     3181 tcttcacctt ttaataacac aaaactcata gatttatcag tcatttcaat agtcccatgg
     3241 aaaaattcgg ctgcatggat cgacttggtg gcaatccatt gcatttcttc cagtacgcac
     3301 atagcatatt cataggcagc cccataagta ttcccggctc caacaaacat atgaaattcc
     3361 gtatttttat gtttttctgc aaattcaagt gctttctgat cattttgttg ccgaacactt
     3421 agtaatactt ccggcatttt ttgtaatgtt tgaataaatt catcatattc aggaaactca
     3481 ccgttatttt tcatcaggcg tgcacccaaa gcaaaaaact gtaactgaag ttcttctact
     3541 aatgtatcgt tgtcagcatc attttcaatc gcataatcca cgatttcccc gagtggtgta
     3601 gcagcatttc ctacaaacgc aatcgtcgtt gcaccttgac tattggcata cttaacaaca
     3661 tcaacggttt cttgagtcgt accggttaat gaagatgtga ttaacactgt atctttatta
     3721 atctttttcg gctgtgaaca aaccacctct gcggctattt catgataata atcaagctct
     3781 gaatgagtgc ttaacagata agctagcggg ctgattgtgg catatgttcc accagagcct
     3841 accaaataaa gatttttaaa tcccttctca ctgattgcat ctgccacctg attgattgtc
     3901 tcgatatttt ttactgcacc atttaatact gatagaaaac gttcttcgct aaaacctaac
     3961 atagttaaaa acctccaagt caataatttg aaaaatatat ttgcaaatgc cggcacatta
     4021 acaaaccact taaccttttt tatataccac ctgttaacgt ttttaatgta ccacttatag
     4081 aaaacgctgt caatagtttt taaccacttt tatttttatt tgtaccaaaa tccttttttc
     4141 atgtataata aacaaaaata gactctggag tgtaacgtat gaatactgaa gaactaacga
     4201 acaaattaat acaagacatt gaacaagaaa aatataaata cgcgcaagct attccttcag
     4261 aacgtgattt tgttcaaatt tatgaaatga atcgttcagc cgtaaaaaaa gccattcaac
     4321 aattagtaaa taaagagtac ctactaaaaa tacatggcaa aggaactttt gttaaaaaaa
     4381 atatccatga acatttgcaa atcaaatttc atggtttgtc tgaattgttg gaacgggaag
     4441 ggattcagcc tagcagctta attttagctt cagaaataaa accggctgga cacattgtcg
     4501 ggaaagcttt acagctggaa ccaactgaag aagtatttca tttggttcgt ttaagaagcg
     4561 gcgacggtga gccaatttct attgaagaca cttatattcc attaaatctc atacctgatc
     4621 ttcaagcttt tgatttccaa attttttcat tttattccac attggctgct tatcattatc
     4681 aaattgaaaa tatctatcag acaatttcca gctcaaaagt gagcaataaa gaagcccgtt
     4741 tgttaaaaac cagcttaaat cgtccggtaa tcagccttgc tatcactgcg gttacgacaa
     4801 atgaacaagc ggttgaatac actgaagtag tggttttgcc tgaattcgcc gccatctata
     4861 ctaaaaatgt tcatcaagga aaggagatga aaatctatgc ccaaattgat taatctgttg
     4921 gcttatgacc tgattgacaa aattattgct tatattcatg aacatcattt aacccccgga
     4981 acaaaactgc cttcggaacg ggacctatca gaaatgttta atgttaatcg aactacctta
     5041 agaggcgcgt tacaaaaact ggtggacaac ggcaccttac gggcgcaaac caatagcggc
     5101 tattatttaa ctgcgccaaa attcgaaaaa aatgcccatc atttctttac accgatagaa
     5161 gattctctgc ttagtaagca gcggctacag ctaaaggcaa cggctctttc accgaaaaaa
     5221 gcagcagctc tttgtccgga actgaatgat ttcaaacagc agcaaccaca agcttattta
     5281 gaatatattg atgcagaagc aattagcttg atgttattaa tccctatgga aacattaaag
     5341 aaaagtaatt tctcggacaa tcctttccaa gattttaaaa gtttggcata tacgcgttct
     5401 caagaaattt ctttaataac tgacaatgac gttgcggcgg acttgctggg agttgacaaa
     5461 gaagaggttc tgttatttgt acgagaggaa ataagagccg gtaaggagtt ggcggcgatt
     5521 gctttaggtt atactattag tcgccgctgc aacatacagg ttaatgttgc tacgaccttg
     5581 caatccggtt ctgttgcaaa gttttaaatc tactatcaga taaggtagaa taatagaaaa
     5641 agatagcagg aggaatgacg atgagtcatt ttaaaggaaa gcaatttca
//
LOCUS       DAFGZE010000124         5661 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.128, whole genome shotgun sequence.
ACCESSION   DAFGZE010000124 DAFGZE010000000
VERSION     DAFGZE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.128"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(57..350)
                     /locus_tag="LTU54_002855"
     CDS             complement(57..350)
                     /locus_tag="LTU54_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HBL8297496.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(378..1043)
                     /locus_tag="LTU54_002856"
     CDS             complement(378..1043)
                     /locus_tag="LTU54_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBL8297497.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(1183..1815)
                     /locus_tag="LTU54_002857"
     CDS             complement(1183..1815)
                     /locus_tag="LTU54_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8297498.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(1822..2889)
                     /locus_tag="LTU54_002858"
     CDS             complement(1822..2889)
                     /locus_tag="LTU54_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBL8297499.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(2886..3617)
                     /locus_tag="LTU54_002859"
     CDS             complement(2886..3617)
                     /locus_tag="LTU54_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HBL8297500.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LTU54_002860"
     CDS             complement(3786..4265)
                     /gene="greA"
                     /locus_tag="LTU54_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBL8297501.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LTU54_002861"
     CDS             complement(4401..5576)
                     /gene="mltG"
                     /locus_tag="LTU54_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBL8297502.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
BASE COUNT         1611 a         1197 c          862 g         1991 t
ORIGIN      
        1 aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct ctttttttat
       61 tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac aagtaaatca
      121 tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc atcaatttcc
      181 actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa accatcatga
      241 acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc tggcagatca
      301 atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc
      361 tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac atcgcctgtc
      421 tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc aggagaagcg
      481 ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa
      541 tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat ttctgctaaa
      601 gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa attatgcgca
      661 atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat cttttcaagt
      721 actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc catttccttt
      781 accatcagag taactaagat actggcttga atatcttctc caatagcgac aattacatga
      841 tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt tgccacaaca
      901 gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc aatagccagt
      961 acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg tccaagacct
     1021 acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt ctcagtataa
     1081 caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa gaaaaataat
     1141 agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca ccaaaccatg
     1201 tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag ccaatatatt
     1261 ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta tttcctgatt
     1321 actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta gttcttcatg
     1381 taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca cttctggttc
     1441 taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat gtgcggtaga
     1501 ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt tttcgtcatc
     1561 aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga ttgcttttgt
     1621 tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt ccggtcgaag
     1681 tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta cttcaatgtc
     1741 ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt catctactaa
     1801 taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga tttctacact
     1861 tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag ctactcgttc
     1921 tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa accctgttcc
     1981 gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata cctctaattc
     2041 attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc ggaacaagtg
     2101 atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg aaatctggat
     2161 tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt tctcttctaa
     2221 attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag cgttgatgat
     2281 ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt tttctgcttg
     2341 ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa ctgaatcatg
     2401 cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg tttgtccatc
     2461 tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa tcattttttc
     2521 ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg atgcacttgg
     2581 gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac gcagcttttc
     2641 ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga caattaatcc
     2701 tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata aacgtgcacg
     2761 cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga aaagtgaaaa
     2821 tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc tggatatttt
     2881 tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat aatcttcaat
     2941 ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt tttcaaagaa
     3001 aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa tcggatggct
     3061 acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat cacaatatta
     3121 tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga aatgacggcg
     3181 atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc gaataattgc
     3241 tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat catgatctgt
     3301 ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac acctttcaga
     3361 ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt gactaagcta
     3421 aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt acgaggatgt
     3481 ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa aagtccagta
     3541 ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac cacaaaaaaa
     3601 cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc atcataccta
     3661 tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa atctaaaata
     3721 cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta gatctttcag
     3781 tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc tggagtagca
     3841 atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg agaatcattg
     3901 gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta ctcttcttct
     3961 tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc atctgcatct
     4021 acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt tgtgattctt
     4081 ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc ggaaagatct
     4141 ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac tgtttttagt
     4201 tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata aactttctct
     4261 accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct tttgaggaat
     4321 atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct aaaaatgatc
     4381 aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa ttccatatgt
     4441 tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac gaaataataa
     4501 tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg attgtcaaaa
     4561 ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac agctgtatct
     4621 tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat atctgactga
     4681 agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt acgatcattt
     4741 tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg tacagtcaaa
     4801 tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt gttcaccatt
     4861 tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc tgggaataag
     4921 taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa cagttctggg
     4981 aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa aaattctttc
     5041 ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc gtacccttct
     5101 ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc ttcttgcaac
     5161 tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc agcttgaaag
     5221 ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt aataatattg
     5281 tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat ctcgacttgg
     5341 actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata acgatacacg
     5401 gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa aatgatcttc
     5461 ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat atctgctgta
     5521 gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc tgacaaaaca
     5581 actcctcctt actatcaagt tcgtaacttt attcattata catgatattt gaattggcgt
     5641 gaatgatttt atttaaatat t
//
LOCUS       DAFGZE010000125         5588 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.129, whole genome shotgun sequence.
ACCESSION   DAFGZE010000125 DAFGZE010000000
VERSION     DAFGZE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.129"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            51..2084
                     /locus_tag="LTU54_002862"
     CDS             51..2084
                     /locus_tag="LTU54_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HBL8297503.1"
                     /translation="MYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSAD
                     TIVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETY
                     PKFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIR
                     NFQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQV
                     ETEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITE
                     GEYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFE
                     QLSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFG
                     KESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDS
                     EEAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLL
                     TSAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVK
                     AASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEI
                     IQNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            2100..2249
                     /locus_tag="LTU54_002863"
     CDS             2100..2249
                     /locus_tag="LTU54_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297504.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKMRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            2323..2916
                     /locus_tag="LTU54_002864"
     CDS             2323..2916
                     /locus_tag="LTU54_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HBL8297505.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            2932..3831
                     /locus_tag="LTU54_002865"
     CDS             2932..3831
                     /locus_tag="LTU54_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HBL8297506.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            3834..4319
                     /gene="ssb"
                     /locus_tag="LTU54_002866"
     CDS             3834..4319
                     /gene="ssb"
                     /locus_tag="LTU54_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBL8297507.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            4685..4945
                     /locus_tag="LTU54_002867"
     CDS             4685..4945
                     /locus_tag="LTU54_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297508.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            5386..>5588
                     /locus_tag="LTU54_002868"
     CDS             5386..>5588
                     /locus_tag="LTU54_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HBL8297509.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
BASE COUNT         2015 a          997 c         1144 g         1432 t
ORIGIN      
        1 ctgtgatcac gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg
       61 aaaaatggaa acaatgggca ttagaacatt tgcctatttt ccccaatcaa taccactatc
      121 atgttcctaa agataagaaa aagcagttta gtgtcgtcaa acgacagctg cagtcagctg
      181 atacgattgt gatcgcgact gatagcgatc gtgaagggga gctgattgcc tggtcaatca
      241 ttcaacaagc aggcgccaat caaggaaaaa cctttaaaag actttggatc aactccttag
      301 aaaaagaagc catttatcag ggattccaac agttacgaga cgcagaagag acctatccaa
      361 aatttgaaga agtacaagca cgtcagatcg ccgattggtt aattgggatg aacggtagcc
      421 cactgtatag tttactcttg cagcaaaaag gcataccagg aagtttttct ttaggtagag
      481 tccaaacccc aacactctat atgatttatc aattgcagga aaagattaga aacttccaaa
      541 aagaaccata ttttgaagga aaagcccaag taatcgctca aaatggcgca tttgatgcga
      601 agcttgatcc aaatgaaacc caagcgacac aagaagcatt tgaagcctat ttaaaagaaa
      661 aaggtgttca attaggaaaa cagccaggta cgatccttca agtcgaaaca gagaaaaaaa
      721 gtgcggctag cccacgtctt ttttctttat cgagccttca gtctaaaatg aatcaactca
      781 tgaaagctag tgccaaagat actttggaag ccatgcaagg gttgtatgaa ggaaaatatc
      841 taagctatcc gcgaacggat accccttata ttacggaagg ggaatatgcg tatctgttgg
      901 atcacttaga cgaatacaag cattttttaa aagccgaagc gattccgact cctattcata
      961 caccgaatag tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta
     1021 caaaaacggt tatgacagca gcggcttttg aacaactaag tccactgcag caggctattt
     1081 atgaacaagt gttaaaaaca acggtggcca tgtttgcgga aaagtatacc tatgaagaaa
     1141 caacgatctt gacgcaagtt cagcagcttc agttaaaggc aatcgaaaag gtgccgctag
     1201 atctaggttg gaagaagtta tttggaaaag agagcgacgg aaaagagaag gaagaagagc
     1261 cgctacttcc taaagtgaca aaaggagaaa tggtgacggt ggatctacaa gttcttgaaa
     1321 aagaaaccaa gccaccacag ccttataccg aaggtacgtt gatcacggcg atgaaaacag
     1381 ctgggaaaac cgttgatagt gaagaagcgc aatccatatt gaaagaagtg gaaggaatcg
     1441 gtacagaagc gacacgggcg aatattattg aaaccttgaa acaaaaggaa tatatcaagg
     1501 tagagaaaaa taagctcgtg gtgacgaata aagggatttt gctttgccaa gccgtggaaa
     1561 aagagccgct gcttacgagt gccgaaatga ccgccaaatg ggaaagctac ttgttaaaga
     1621 tcggtgaacg aaaaggaaca caaaccactt ttctagccaa tatccaaaaa tttgtctctc
     1681 acttattgga agtcgtacca gggcaaatcc aatccactga ttttggatcg acgttacaag
     1741 aagtgaaagc agccagtgaa aagcaagaag ccgcgcgtca tttaggaatt tgtccaaaat
     1801 gccaggaaca agaagtctta ctctatcaaa aggtggcagc atgtacgtct gaagcctgtg
     1861 atttcaaact atggacaacg attgccaaaa agaaactcac agccacacaa ctgaaagaga
     1921 tcattcaaaa cggccgaaca agccaatcag tcagaggact aaaaggtcag aagggttcgt
     1981 ttgaagcaac gattgtttta aaagaagact tcactacagg ctttgaattt tctgaaaaga
     2041 aaaaaacgaa cttcaagaaa agaacacgac gcactacaaa ataatggagg gttaatctta
     2101 tgcgtaatac aacacacaaa caacaagaaa tcgaaaatca aaaagccaaa attcgttcag
     2161 aacaggaaaa aatgcgtgcc agcaaacgaa gactgcagca attgaagaac cagcagcgga
     2221 ttcaaaaaga aatcgaatta gatcgataaa ttcctttctt gttaatataa aactaacatg
     2281 ttatagttgt gttagtttta tattaacttt aggggaagag taatgaaaga caaaatccaa
     2341 tgtatttttg ataaacataa tggcaaccta tccttgaagg atgctagaag tgaaggatta
     2401 tcgcctatga cgttagatcg attagccgaa aaggataaaa tagaaaaggt agcaccaggt
     2461 ttctatacct taaaggatgc gttcgtggat gaattgtatc tcgctcagag tatctattcc
     2521 agagggattt tttctcatga gactgcgctc gatctttttc aagtaggaac atatatccct
     2581 aaaaagatca atatgatgtt tcctaaagga tacaatgtat caaaggagaa attagatcag
     2641 tatgccattc aaccgcatta cactaataaa gaaaattttg acctgggagt cacagaaaca
     2701 gaaagctttt acggcaataa aattttagtc tatgatttag agcgaacgtt atgtgacatg
     2761 tggaatccaa gatacaaagc aagcgtagag gtaaaacaag aagcattaaa agaatatatg
     2821 acaagtacca gtagaaatac aaataagcta agaagataca tggatatttt aaaatcacca
     2881 aaagaaatga ctttgtatat gttgccactt tattgagagg agtaaagatg aatgacaccg
     2941 acacaattta aagagaaaac aagaaatttc gcagcagaga aacatttaaa cccacaaatt
     3001 gtccaaagat attatatgat ggaaaaatta ttagaatgta ttgttgaatc gccttatcat
     3061 gatgattttg tattaaaagg gggatttctg attggatcaa aatacgggtt agaaaaccgt
     3121 tcaaccgttg atattgatac gacttatcga aattcgaaac taacaaaaga aaagttagaa
     3181 actgtttttt ctgaattaac agctaatcct acaaaagatg gaattcgttt tgaattaaag
     3241 ggattaacag agacgaggga agccgattat tatccaggtt ttcaagcaaa gtttattgct
     3301 tatcttgaaa atgcacgtgt accttttaaa ttagatatta cttcaggtga ttctattatt
     3361 ccagcagcat tgatgtatga acacaaatta atgtttgaag ataaaacgat tcatattcca
     3421 gcttatccta cggaacaaat tctagctgaa aaattacatg ccacgtttag ttttggaaaa
     3481 gataattcgc gcatgaaaga tttttatgac ctgtatatga ttccgaaatt agaacgtatc
     3541 gatgagaaaa atttgtataa gagtgttcaa ggaacattca aggaaagaaa taatcaatcg
     3601 gtgtttaagc atttgtatga tcaggaaatg cctcaattgg tgaaagatga gggcttgaga
     3661 gaaaagtgga aaagatacca acaagaaaac tattttgcca aagacatttc tttcgaatct
     3721 acactaactt ccgttgattc cctgatgcaa tcaattgtag agcaagagaa ggcagaacga
     3781 aaacaaaatt ttaagagatc acaacaaatg aatcaagaaa tggagagata aagatgatca
     3841 ataatgtaac tttagtaggg cgattaacta aagatccaga tttacgttat accgcaagtg
     3901 gcacagcagt ggcgactttt tccctagccg ttaatcgtaa tttcacgaat caaaacggca
     3961 atcgagaagc tgattttatc aattgcgtga tttggcgcaa accagcagaa acgatggcga
     4021 cattagctaa aaaaggcagc ttgatcggcg ttgtcggacg aattcaaaca cggacttatg
     4081 acaaccagca aggacaacgt gtgtatgtga cagaagttgt ggccgataac ttccaactat
     4141 tagaaagtaa agcagcgaca gaaagccgta tgcacgctga tcaatcgtcc agcagtccaa
     4201 gccctaccac ctttgaacaa cgggatacag cgacgcctaa caacaatgga ttgaacgctt
     4261 cacagaatcc atttggtgga caatcaatcg atattagcga tgatgatcta ccgttctaag
     4321 tagcattttc tattggttgc atttgctacg gtttatgtta taatttagac aacaaaaaga
     4381 attttcaatc tataaccaaa aaccactagc tggcgggcta gtggctttct tttacgtttc
     4441 tattataaga attaacgggt aggcttactg tctttcttgg caaatttaca atcaagccat
     4501 ttgcagatat agtgcgcggc tacttctgcc atgattgcaa aaacaaaatc aaaaagaatt
     4561 ttcaaaccta taacctcctc cccttgccag attaggccgg ggtagcagac agcaagacta
     4621 ttatagcata attgaaatat ccaaatgatt ggatttttgc tataatttta gcgaggtgat
     4681 tgtgatgaac aaatatgttg gtttgcttga taaaatccga gtaataaaaa cgcaaccgtt
     4741 attggttcgc tttactttac aaacgattca tgaatcgatt aattgtgtag ttgcagatat
     4801 tgaaattatc gataaattgc taattatgga tgatggaaaa tacaatattg ccgtcacagg
     4861 tcacttcaat aaacgaaatc agctagttat tgctagcatg tatgttagaa atcccgatca
     4921 ttttacgcga tcgatgggca tataaataaa agaagagttg tagtcctaaa tgaggattgc
     4981 agctcttttt gttttacata gaaacaggaa aagacctttt taggaagaca caagtgcctt
     5041 ctcaattgaa aaacctttct ctcactccaa ccgccaccgc cgttcgagaa gaaaggaagt
     5101 gaacgctgca cgttcactaa gatatggtgt cttgcaaagc aagacaaaat agacaaaact
     5161 tcctctcacc aaaaggttcg ttcctgttcg ttatctcttt attgatttgc cagaccggca
     5221 acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca cgctgcgcgc
     5281 ccttgtcctt gtcgcttttc gttgatttat tctggccaac aaatcaataa agaaagaact
     5341 aacgaaagga agtaaaacct ttcctaagag gaggcagaat caaacatgcg aaagaaattt
     5401 gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc acatactgtt
     5461 gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct atcgtttgta tcaatgtcca
     5521 acatgcggga aaaaatatac cttgtcttat ggcgattaca ttttgattga tgagaaagga
     5581 aagaaacg
//
LOCUS       DAFGZE010000126         5300 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.130, whole genome shotgun sequence.
ACCESSION   DAFGZE010000126 DAFGZE010000000
VERSION     DAFGZE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.130"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(86..748)
                     /locus_tag="LTU54_002869"
     CDS             complement(86..748)
                     /locus_tag="LTU54_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8297510.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            932..2578
                     /locus_tag="LTU54_002870"
     CDS             932..2578
                     /locus_tag="LTU54_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBL8297511.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2569..3651
                     /gene="uxuA"
                     /locus_tag="LTU54_002871"
     CDS             2569..3651
                     /gene="uxuA"
                     /locus_tag="LTU54_002871"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBL8297512.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3759..5279)
                     /locus_tag="LTU54_002872"
                     /pseudo
     CDS             complement(3759..5279)
                     /locus_tag="LTU54_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331751.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1717 a          900 c         1009 g         1674 t
ORIGIN      
        1 aaagtcaatg gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa
       61 aaatagaaga ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa
      121 cagtttcctg ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt
      181 ctggtttttt atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc
      241 atggaagccc ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt
      301 catctaacca gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata
      361 aattatctgc acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt
      421 ctaaaatcgt ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca
      481 tgatttgctc ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa
      541 ttttggaaac atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt
      601 gaatcaatgc ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt
      661 cagatatttt ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat
      721 aagcttgtga ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt
      781 agtataacag aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta
      841 taaatagtaa aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca
      901 aaaactaata tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt
      961 tatccctgaa ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag
     1021 agaaaatgga caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg
     1081 atttcatgca gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat
     1141 tgtttgtgaa acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt
     1201 gattcttgaa gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac
     1261 agctgacagt ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca
     1321 ctttgaaaat cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt
     1381 gaaagataca aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa
     1441 gaagccatca catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg
     1501 agcttggccg attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg
     1561 tacttctttg ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt
     1621 tatccaatac ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat
     1681 caccccgaat ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat
     1741 gtcgattttc caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat
     1801 ccattattta atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg
     1861 ggtattcttg actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg
     1921 ccttaatcct ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat
     1981 agctgaggag atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga
     2041 aggcctccca gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt
     2101 aattactgtg cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga
     2161 tacctctcaa aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga
     2221 agttaaagac ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata
     2281 ccttattggc attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac
     2341 agaccttcag cataagcttt ccgatattca actgggtacg caagatacat cacatattca
     2401 tgagaaaatg gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc
     2461 agagcttggc agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt
     2521 acgaaaaaca ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact
     2581 tttagatggt acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct
     2641 gcggtcaaag ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg
     2701 gagaggatca aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt
     2761 gaaagcgtca atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc
     2821 gaaaactata agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat
     2881 aattttatgc ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc
     2941 tcatccacgt tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg
     3001 cgcatcgaac attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct
     3061 cctatcaaat ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat
     3121 tttgcctatt ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct
     3181 gtacatccag atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga
     3241 gaagatttag attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt
     3301 acagggagta ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc
     3361 caaagagatc gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat
     3421 ttctatgagg caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa
     3481 acaatgtatg acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg
     3541 aatgagacag gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta
     3601 aatggattat gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa
     3661 ctgtaatttt attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg
     3721 ttgttaataa gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct
     3781 tttttcgatt gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc
     3841 ataatcactt ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg
     3901 atatgagaag gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata
     3961 aaatcatttt gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga
     4021 tctttactga tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt
     4081 ttgtagcgat catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct
     4141 aaaataactg gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg
     4201 gcggtttgga cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc
     4261 gtatttggtt gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat
     4321 gagtaaagaa tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc
     4381 accatttctc ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc
     4441 tccaagcttg tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact
     4501 aagatgcgtt gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca
     4561 ataaaaaagt caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt
     4621 gcaggaaaat tctctttaca tatttttaat agaaaatata atttatctat cataccgcta
     4681 tttagtcggt caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc
     4741 ggatcttcat cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat
     4801 gctgcatatt gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag
     4861 gagatagaca aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa
     4921 taagaggcac tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg
     4981 aacaaagaag tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga
     5041 taatagcagt aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta
     5101 tcaatatgca gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat
     5161 agtaaaaaat ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag
     5221 aaacgatgtt cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag
     5281 ataaacgttt taatcgcttc
//
LOCUS       DAFGZE010000127         5201 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.131, whole genome shotgun sequence.
ACCESSION   DAFGZE010000127 DAFGZE010000000
VERSION     DAFGZE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.131"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..172)
                     /locus_tag="LTU54_002873"
     CDS             complement(<1..172)
                     /locus_tag="LTU54_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL8297513.1"
                     /translation="MNDFTTEILKTLANKGDLNGLFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNS"
     gene            629..2140
                     /gene="gadC"
                     /locus_tag="LTU54_002874"
     CDS             629..2140
                     /gene="gadC"
                     /locus_tag="LTU54_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HBL8297514.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            2153..3553
                     /locus_tag="LTU54_002875"
     CDS             2153..3553
                     /locus_tag="LTU54_002875"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBL8297515.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(3718..5153)
                     /locus_tag="LTU54_002876"
                     /pseudo
     CDS             complement(3718..5153)
                     /locus_tag="LTU54_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         1626 a          858 c          981 g         1736 t
ORIGIN      
        1 gagaattacc cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta
       61 actccgtttt gagaagcgta ttgacagctt tttccaaatg gacacggaat aatccattca
      121 aatcgccttt gttcgctaga gtctttagga tttctgtagt aaaatcgttc atatggaagt
      181 cctcttttct gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct
      241 atttacacaa aatattttac actctcaaca aaagtatctt cgattaatac ttgctcaagt
      301 tatgtgcagc tacgaaaaag taggtaccga ggtactttct ttttcgcagc tttttttgga
      361 cttatgagaa gtacattcgc tggaagaata cgatataaaa cgagcttttt cactaaagat
      421 attaatagag tgaaagaaac aagcttttgt tgtcagtttt ttcacttttc taaaaatgaa
      481 aataaaaaat tctggaagtg gagaagaatt ttgcttgcta tactttgtct tgtagaaaag
      541 aggtagacaa aataatgcaa gctcaaataa gcgattttcc ataaccttgg taggaatgac
      601 aaattaaata ggaaagggaa gtaataatat gaataaaaaa aagttatctc tattcggttt
      661 ttttgccctg acagcttcca tggttttgac tgtttatgag tatccgacct ttgcaacatc
      721 acaattacat ttggttttct ttcttctttt gggaggtatt ttatggtttc taccagttgc
      781 tttatgtgct gcagaaatgg caacagttga aggatggaaa gatggtggga ttttcagttg
      841 ggttagtcaa actttaggag aacgttttgg ttttgcagct atttttttcc aatggtttca
      901 aattacagta ggttttatta caatgatcta cttcatacta ggagcgctgt cgtatgtaat
      961 taattttcca gagcttaata cagatccatt tatcaagttt attggtgtat tagtcatttt
     1021 ctgggtactg actttttcac agttgggcgg gaccaatcgt accgctaaaa ttgctaaagc
     1081 aggatttgtc attggaattg ttattccttc gatcattctc tttggtttag cagcagccta
     1141 ctttatcggc ggtaatccga ttcaaatggc tattaatgcg aaagctttta ttcctgactt
     1201 ttctcagctt tcaactttag tagtatttgt atcttttata ttggcttata tgggcgtaga
     1261 agcttcagca tcacatatta atgagctgga aaatccccaa agaaactacc cattggcaat
     1321 gtttttacta gtgattttgg ccatcgcctt agatgcaatc ggcggtttct cagtagcagc
     1381 ggttattcca caacatgact tgtctctcag tgctggtgta atccaaacat ttgaaacgct
     1441 tattttacat tttaacagtc atttacattg gttggtaaaa gtgattgctc tcatgattgc
     1501 ctgtggcgtt atgggtgaag ttagttcttg ggtagttggt ccatcacgag ggatgtttgc
     1561 agctgctcag cgaggtctgt taccagtttt cttccggaaa actaataaac aagacgttcc
     1621 agttccgttg attatgatac aaggaattat cgttactatt tgggcagcag tcttaacttt
     1681 tggtggagga ggcaacaatt tatcattctt agtagcaatc tccttaactg ttgtcattta
     1741 tctcgtagga tatttactat ttttcctagg ttactttgta ttagttttta agaaaaaaga
     1801 cttaaaacga acttacaatg taccaggaaa aactgttggc aaaacgatta tcggtggaat
     1861 tggttttatt ttatcagtgt tcgcattgct tatctctttt gtaccaccag cttcaattgc
     1921 agctagtgag caccgaacat accaaatcat actacttatt agctttgtag tgactgttct
     1981 tttacctttt atcatttatg aagtacatga taaaagtgct cacacaacta ttgaggaacc
     2041 tactcacgtg aaagctgaag acgttaatcc cgctgtttat ccagctgcta gaggagagca
     2101 ccacgttaca aaaaatgatg aacatatttt aaaacattag gaggaattca ttatgttata
     2161 cggaaaagat aatcaagaag aaaaaaacta tttggaacca atttttggct ctgcaagtga
     2221 ggatgttgac ttgcctaaat ataagttaaa caaagaatcc attgaaccac gaattgctta
     2281 tcaattagta caagacgaga tgttggatga aggaaatgcg cgattaaact tagctacttt
     2341 ttgtcaaacg tatatggaac ctgaagcagt gaaattgatg acccaaacgt tagaaaaaaa
     2401 tgcaattgat aaatcagaat acccacgaac aacggaaatt gaaaaccgct gtgtaaatat
     2461 gattgctgat ttatggcatg ctccaaataa tgaaaaattc atgggaactt caacgatcgg
     2521 ctcttcagaa gcctgcatgc tgggtggtat ggccatgaaa tttgcttggc gtaaacgtgc
     2581 tgaaaaatta ggtcttgata ttcaagcaaa aaaacctaac ctggtgatct cttctggtta
     2641 ccaagtttgt tgggaaaaat tctgtgtata ttgggatgtg gaactgagag aagtcccaat
     2701 ggatgaaaaa catatgtcaa ttaatctaga tactgtcatg gattatgtgg atgagtacac
     2761 aattggtatt gtaggtatta tgggtattac ttacactggt cgttatgatg atatcaaggg
     2821 tctgaatgat ttagttgaag ctcacaataa acaaactgac tataaagtat acattcatgt
     2881 tgacgctgca tcgggtggct tttatgcacc atttactgaa cctgatctag tttgggattt
     2941 tcaattgaaa aatgttatct caattaattc ttcaggtcac aaatatggtt tggtatatcc
     3001 aggtgtgggt tgggtcttat ggcgtgacca acaatactta ccagaagaat tagtatttaa
     3061 agttagttac ttaggtggag aaatgccaac tatggctatc aacttctctc atagtgcagc
     3121 acaactaatt gggcaatact acaactttgt acgctatggc tttgatggtt atcgtgatat
     3181 tcaccaaaga actcatgatg ttgctgttta tttagccaaa gagatcgaaa aaactggtat
     3241 ttttgaaatc attaatgatg gatcagaatt gccagttgtg tgctataagc tgaaagaaga
     3301 tcccaatcgc gaatggacac tatatgattt atctgatcgt ctgttaatga agggatggca
     3361 agtcccagcc tacccattac ctaaagactt ggatcaatta attattcaac gcttagttgt
     3421 tcgagcagac tttggaatga acatggctgg tgattatgta caagatatga accaagcaat
     3481 tgaagagttg aataaagctc atattgttta tcataaaaaa caggatgtga aaacgtacgg
     3541 ctttactcac taaaattctt cagagaaata taaaagaagt taattaacta ataaaaggag
     3601 ttgtgattac cacacagctc cttaaatcta tttgaataaa tatatgatat tcatttgtta
     3661 cactctcaat cttagctgtt gttctattca tcattactct tattgtaagt agttcagtca
     3721 aatttgcttg ttcaattgat cgataacagt atgaattgtt tgaaaagtaa tcggatatat
     3781 aattgttttc tcactaccta agccttcttg gtataaagaa ctatcgcaaa gtataatccg
     3841 ctgagtacaa aagaaagtgt cgtctactat ttctctaagt tgtttacaaa tgatattact
     3901 aatagaaaag tgttctccca aaatgtcttc taactgttgt cgatagtata aatacctaaa
     3961 gtctgacgga gctactatga acacttcaat tggagatttc cgtttatgct catttaaaag
     4021 acttaaacta atggttagtt tacgaataga attctcattc caacgcagaa ataaattgtt
     4081 ttgttttcgc cattgttcaa gaatttcatg aacttctaag tacaaagcgc gttgctcttc
     4141 atttaacaag taagtcttat caggttgaaa tatctgaata tcagcccaaa gtgagcggat
     4201 aaacgggaga agtgctttat caaaaagtat ctgattttct tcagatatat tcagagataa
     4261 gataagtttt tgactgagct caatcaccaa agggttatct gctacatggt tttgataaac
     4321 aacgttcaag tctttttgta acaattcaaa gttatcgcat agataatttc tagagttgaa
     4381 tagaaagtag atataaaggc attcatcttc gtcatgacat aaagagaaag aaagttgctc
     4441 taatcctttc tgtattagct gaaacaaggg agtatgtcgg gcaactcttt tttcttcctg
     4501 actaaaatat attgggttag tcttagatct ctgcaaaccg atagcgatac ctaataaaat
     4561 aaattgtttt tcaactgtag ggtaacttct tgaaaaaaat gtttttctac ataatcaatc
     4621 aattgttgac cgctttgaat aattgattta tttagctgga tcaactctgt ccgatttgtc
     4681 tcatagatta tcgttagtaa caaacttcga taatcctttt caggaatttc aaaatggcca
     4741 tcagcattta agtactgcat ttgtttaaaa aaattaatca actcatttcg taagttgtaa
     4801 actttgctta acgaaatgaa ggtagtatca gctatattac tccaattgtg ttcccctgat
     4861 aaataatgtc ctagtacatt gaaaaagtca gagcgctgat agatgagttg agtgagtgcc
     4921 aacctcgaaa acgaagagtc gaatgcgact gtaaagctac ttctagattt ctccactgaa
     4981 ataatttgct gatggagtat gtcggtaatg cattccaagt cgttagtgac agtgagtggg
     5041 caaacgttca attgatctgc tatttcgcga atctgaatgg tccctttttc gtgtaattgc
     5101 tcacaaatgt aaacttgacg aaaaatactt ttctccagat aggtttccaa catagtactc
     5161 ctcctagtca ttttatacat aactattata actctaccaa t
//
LOCUS       DAFGZE010000128         5191 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.132, whole genome shotgun sequence.
ACCESSION   DAFGZE010000128 DAFGZE010000000
VERSION     DAFGZE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.132"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            137..748
                     /locus_tag="LTU54_002877"
     CDS             137..748
                     /locus_tag="LTU54_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL8297516.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="LTU54_002878"
     CDS             821..1750
                     /locus_tag="LTU54_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBL8297517.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2645
                     /locus_tag="LTU54_002879"
                     /pseudo
     CDS             1819..2645
                     /locus_tag="LTU54_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033645.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            complement(2693..3871)
                     /locus_tag="LTU54_002880"
     CDS             complement(2693..3871)
                     /locus_tag="LTU54_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL8297518.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKSFTEEVTSFKERELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERG
                     VKNVLLFITDGLKGMAGAVQRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKKAALEARGAFAEKWKTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            3894..4067
                     /locus_tag="LTU54_002881"
     CDS             3894..4067
                     /locus_tag="LTU54_002881"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297519.1"
                     /translation="MCCGNSILLKGLPFFYLHKIFYTLGLKERGLSGVKMVISDAHIG
                     LTKAVKEYLLCAS"
     gene            4098..4499
                     /locus_tag="LTU54_002882"
     CDS             4098..4499
                     /locus_tag="LTU54_002882"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297520.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(4706..>5191)
                     /locus_tag="LTU54_002883"
     CDS             complement(4706..>5191)
                     /locus_tag="LTU54_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8297521.1"
                     /translation="LKEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKE
                     QQVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFS
                     NNQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVI
                     K"
BASE COUNT         1641 a          879 c         1003 g         1668 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt catattggac atggtttggg
     3481 gcgtgtagtg atgcccatac attttctcaa tcaagtctgc gatttccgac atggtaatac
     3541 cttttcggaa gaggtgaatg acggtctcct ctaaggtatc attcgtccgt ctataagcag
     3601 gaacagtctg ttgcttgaac tcgccgttgc ggtcgcgcgg aatctggaga tgaagttccc
     3661 cgtactcggt cttgaccgta cggtcatagg agccgttacg agaattaccc gtgttaaaac
     3721 caatgcgatc gtacttttcg taatcgagga aagccgttaa ctccgttttg agaagcgtat
     3781 tgacagcttt ttccaaatgg acacggaata attcattcaa atcgccttta ttcgctagag
     3841 tctttagaat ttctgtagta aaatcgttca tatggaagtc ctcttttctg tgaatgtgtt
     3901 gtggtaactc tattctactg aagggacttc cttttttcta tttacacaaa atattttaca
     3961 ctctcggatt gaaagaacgc ggtctttcag gagtaaagat ggtcatctcg gatgcgcata
     4021 tagggctcac aaaagctgtg aaagaatacc ttctttgtgc gagctagcag agatgccaag
     4081 cacatctcct tcgcaacatt tccgacaaat tctccaagaa aatctcaagc gatatcaaag
     4141 aagactttaa agccatcttc aaaacggcaa gcatcgagct ggctggagaa cgtaaacaac
     4201 ttattatgga taaatatggg gaagacaaga aagtcgcaac tgcctgtaaa atattggatg
     4261 agggcttcga agatgccatg caagtaatgg ttttaccaga aaatatccgt caacgattga
     4321 gaacaacgaa tgttcttgaa agactaaatg aagaagtacg ccgtcgtgaa cgtgttgtcc
     4381 gaattttccc aaattgtgat tctgttattc gtatcatcgg tgctttattg atggaaaagg
     4441 atgacgaatg gacttctgca cctagaaaat atcttgaatt tgatcgtaac gagatttagc
     4501 acattaagcc gccaatagta taatatactg gtgagaagta tcaagagacg cttcgctttt
     4561 tgctcttgac acttcttacc agcatattgg accgtagcta ggctcaacaa ggcaaaacat
     4621 cgtactttga gatttccttt ttacacaaga ttttggactt gacacagccc ctatggttct
     4681 taccttgttt atgcttattt aaatcttact ttattacagt actttcaaac ttcgttttgt
     4741 aagccgggta ttttgatatt tcataattat gtccctcttt ttgaatatat tctttgatat
     4801 ctgcttgttc atctacactt tctttgcgta aaatacgttc gatcattgag cagcagtgca
     4861 tgtaaagcaa gaacaattga ttgtttgaaa attcgatccc cattagtttt tcgagttctt
     4921 tgacggcctt ttgtaccaat ctcaaaacct tcattgcatc aaggatcgtt aaattttcta
     4981 cgatcttgtt aatcgagaaa ttaaagtatt gagcacaatt ataggcgata aatggagcat
     5041 ttgattctat cacctgttgt tccttggcgt attcataaat tttttctgcc aaaaaggttt
     5101 ttcctgaacc gcttacaccc atcaataaaa tcggtaatcc gttatgagga tacaagacag
     5161 ccgtcttgat ttgatcgatc gcttccttta a
//
LOCUS       DAFGZE010000129         5106 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.133, whole genome shotgun sequence.
ACCESSION   DAFGZE010000129 DAFGZE010000000
VERSION     DAFGZE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.133"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..968
                     /locus_tag="LTU54_002884"
     CDS             <1..968
                     /locus_tag="LTU54_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBL8297522.1"
                     /translation="DYYKVFAIIEKLFWISLPLCLIERFMVTYPTGTIVGDMIGGVFW
                     NYSGCNMPLNLILCVTVTVVTIGYFKGEKNFIHFILCVCAALFMSALAELKVFIPELF
                     IIFVAIVFTLKVDFKVVIKILSCVILVSIVLSFFITIFITLNDNGQSYADNFTIQGLL
                     EYASRDSGYDGVGDLNRITGISRVVDEIFNNNFRLVLMGVGIGNAEFTNFFVSEFYKS
                     FGNLHYQYFHHIWLFIEVGILGVSIFLFFIIKTFFNAKRSFMKDKDASLVKVIAIMSV
                     FFFVYNTTLRGETTGPLIYMILAMPEVVKRKEHYYSEKNTKGEIL"
     gene            965..1684
                     /locus_tag="LTU54_002885"
     CDS             965..1684
                     /locus_tag="LTU54_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL8297523.1"
                     /translation="MIPKKIHYCWFGGNPLPKKDRKCIESWKKYCPDYEIIEWNESNY
                     DLNKNRYMADAYKERKWGFVPDYARFDIIYNHGGFYLDTDVELIKSLDILRENHAYMG
                     FEGEIWVNGGIGFGAEEGHSVIKGLRDIYDSLSFYQNDGTLNLKPSPYYITDYLESIG
                     LVRDNSEQIINGLHIYPTDYFAAKDYDTGKIYKTSNTISIHQYNASWKTPKQKFVYRL
                     KSTIGQKKFNRLLALKKFLHL"
     gene            1699..2784
                     /locus_tag="LTU54_002886"
     CDS             1699..2784
                     /locus_tag="LTU54_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL8297524.1"
                     /translation="MKKICFISGVISRSGGTERVGSIIANSLSDLGYDVTILSFWNSG
                     EPYFGLDKTIKVDYMLNPKTEGKLYRTYLYPVWKLHKYICKNEFDIVIDIDTLLSLYT
                     TRAIKGTKCKLISWEHFNYWTMEMLKEKKRFKAKKLIKKYAKRLVVLTEEDRQKHIDE
                     YSLSEDFVITMPNPCISNIEYEYHYDKKRFLAVGRLVEEKNFADMISAWKIVEKKVDD
                     WDLIIVGKGELEKDLRQQIKDLNLQRVTIAGHSDHVEEYYKSASCYVLSSVYEGFPMV
                     ILEAQSYGLPVISYDCKTGPRDLVHHNFNGMLVEDKNIDQLAKSMIMFTKNTDLAMRM
                     SLNAYNNVQKFNLKEITKQWVALIENV"
     gene            2920..4452
                     /locus_tag="LTU54_002887"
     CDS             2920..4452
                     /locus_tag="LTU54_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8297525.1"
                     /translation="MIINRKQNAKRNIITGTIFKLYQIIIPFFMRTLMIYQMGMEYAG
                     LNNLFTSIFQMLNLAELGIGAALTVSMYKPIAEDNRKEICALLNAYKKYFSIIGWIVF
                     TLGLLCIPFLRFLVKGSIPGNLNMTVLYLMYLINTVLSYWLFSYRKSLIYAHQRNDVI
                     NVSMLITFTFQFVMQAYVLIFLKNYYLYLLASILGQIVLNLMCAVVSQKMYPDYTPVG
                     ELEKETIDDIIKKVKGLVTNKVGGTILRSSDSIIISSFLGLSVLAIYQNYYFIISAIM
                     SIFAIIYESCIAGIGNSLIVESQEKNYIDFCDITTIIGWFLAISCACFITLFQPFMII
                     WVGSDNLMSISFVIFMIVHFFLYEIDQLIGLYKDAAGIWYADRFRPLITAMINIVLNI
                     ILVNWFGLYGVLAATIVSVLLVDLPWLLYNVFQNIFKFESAGKYVWKLISLCVIAFVA
                     SYLSYFACSYVNIGNFKVISLVINLVISVVISILVILLFNIRNKKFKYILKVLTSVLV
                     KR"
     gene            4630..4851
                     /locus_tag="LTU54_002888"
     CDS             4630..4851
                     /locus_tag="LTU54_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL8297526.1"
                     /translation="MGSSERIKIGNNVFIGMKSRILKGVHIGNNVVIGANSLVNKDVS
                     DDCVVAGNLAKVIMSLDEYYKKINNMKNP"
BASE COUNT         1765 a          546 c          986 g         1809 t
ORIGIN      
        1 aagattacta taaagtattc gctataattg aaaaattatt ctggatttca ctgccacttt
       61 gtttaattga aagattcatg gtcacctatc caactggaac aattgtagga gatatgatag
      121 gtggagtttt ttggaattat tcaggttgta atatgccatt gaatttaatt ttgtgtgtga
      181 cagttactgt tgttacaatc ggatatttca agggagaaaa aaatttcatc cattttattt
      241 tatgtgtttg tgcagcactt tttatgtctg cattagctga attgaaagtt tttattcctg
      301 aattatttat aatatttgtt gcaatagtat tcactttaaa agttgatttt aaagtagtga
      361 tcaagatatt atcatgcgta atacttgtgt cgatagtatt atcctttttt ataacaatat
      421 ttattacatt gaacgataat ggacaaagct atgctgataa tttcacaatt caaggtcttt
      481 tagaatatgc gtcaagagat tctggatatg atggtgttgg tgatttaaat cgaataactg
      541 gtatctctag agtagtagat gaaatattta ataataattt tagattagtc ctaatgggag
      601 taggcattgg caatgcagag tttaccaatt tttttgtcag tgagttttat aaatcgtttg
      661 gtaatcttca ttatcagtat tttcatcata tttggttatt tatagaagtt ggaatattag
      721 gagtttcgat attcctattt tttataataa agacattttt caatgcaaaa agatctttta
      781 tgaaggataa agatgcaagt cttgttaaag ttattgcaat tatgtcggta ttcttttttg
      841 tgtataacac aacacttagg ggtgaaacaa cggggccact gatttatatg attttagcca
      901 tgcctgaagt tgtgaaaagg aaagagcatt attatagtga aaaaaataca aaaggagaga
      961 tattatgatt cctaagaaga tacattactg ttggttcggg ggaaatccac ttccgaaaaa
     1021 agatagaaag tgcatagaaa gctggaaaaa gtattgccct gattatgaga ttattgaatg
     1081 gaatgaatca aattatgatt taaataagaa ccgttatatg gccgatgcat ataaagaacg
     1141 taaatggggt tttgttccag attatgcaag atttgatatt atttataatc atggtggatt
     1201 ttatttagat acagatgttg agctaattaa gtcgttagat atacttcgtg aaaatcatgc
     1261 ctatatggga tttgaagggg aaatctgggt taatggcggt attggttttg gtgcagaaga
     1321 aggacattct gttattaaag gtttaaggga tatttacgat tcattaagtt tttatcaaaa
     1381 tgatggtaca ttgaacttga aaccgagtcc gtattatatt acagattatc ttgaatctat
     1441 tggattggtt cgcgataaca gcgaacaaat aataaatggc ctgcatatct atcctacgga
     1501 ttattttgct gccaaggatt atgatactgg aaaaatttat aaaactagta atacaataag
     1561 cattcatcaa tataatgcat cttggaaaac acctaaacaa aaatttgttt atagattaaa
     1621 atcaacaatt ggacaaaaaa aatttaacag gttacttgca ctgaaaaaat ttttacactt
     1681 ataaaggaga aaagtacttt gaagaaaatt tgtttcatta gtggtgtaat ttctcgttct
     1741 gggggaactg aaagagtagg gagtattatt gccaatagct tgtctgattt aggttatgat
     1801 gtcactattt taagcttttg gaatagtgga gagccttatt ttgggttaga taagacgatt
     1861 aaggttgatt atatgttaaa tcctaaaacg gagggaaaat tatatagaac ttatttatat
     1921 ccggtttgga aattgcacaa gtatatatgc aaaaatgaat ttgatatagt aattgatatt
     1981 gatactttgt tgtctttgta tactacaagg gcaattaaag gaacaaagtg caaattgatt
     2041 tcttgggaac attttaatta ctggacaatg gaaatgctga aagaaaagaa gagattcaag
     2101 gctaaaaaat tgataaaaaa atatgcaaag aggcttgtgg ttctcacaga agaagatcgt
     2161 caaaaacata ttgatgaata tagtttatca gaagattttg ttataactat gccaaatccg
     2221 tgtatatcta atatcgagta cgaatatcat tatgataaaa agagatttct tgcagtaggg
     2281 cgattagtgg aagagaaaaa ttttgctgac atgataagtg cttggaaaat tgtagaaaaa
     2341 aaagtagatg attgggatct tatcattgta ggaaaaggtg aattagaaaa agatcttcgc
     2401 cagcagataa aagatttaaa tctgcaaaga gtaactatag cagggcattc agaccatgtg
     2461 gaggaatatt ataaatctgc atcttgttat gtgcttagtt ctgtttatga aggatttcct
     2521 atggttatat tagaagcaca gtcttatgga ttaccagtta tttcttatga ttgtaaaact
     2581 ggtccgagag atttggtaca tcataatttt aatgggatgt tggttgaaga taagaatata
     2641 gaccaattag ctaagagcat gataatgttt actaagaata ctgatttagc aatgagaatg
     2701 tcactgaacg catataataa tgtacaaaaa tttaatttga aagaaattac aaaacaatgg
     2761 gttgctttga ttgaaaatgt atagtttttt gtggttgtcc aaacatgaaa attagcgtaa
     2821 ttaatcaaag agctagaaga aacctattac atttcaagat tttagaaatt atatagtatt
     2881 aggtaacaat aaaaaataga aagtttggag ccatatgata tgataattaa tcgtaaacaa
     2941 aatgctaaaa ggaatataat tactggaaca atttttaagt tatatcagat tatcattccc
     3001 ttttttatgc gtacacttat gatttatcag atgggaatgg agtatgctgg attgaacaac
     3061 ctgttcacat ctatatttca aatgttaaat ttagcggaat tgggaatagg tgctgcacta
     3121 actgtaagta tgtataaacc tatagcagaa gacaatagaa aagagatttg tgctttactg
     3181 aacgcatata aaaagtactt ttcaattatt ggatggatag tgtttacatt aggattgtta
     3241 tgcattcctt ttttgagatt tttagttaaa ggttcgattc ctggaaattt aaatatgaca
     3301 gttttgtatt taatgtattt gataaatact gttttgtcgt attggttatt ttcatataga
     3361 aagagtctaa tatatgcaca tcaaagaaat gatgtaatta acgtgtctat gctaattact
     3421 tttacattcc aatttgtaat gcaggcatat gttctgattt tcttaaaaaa ttactatttg
     3481 tatttattgg cttctatttt gggacaaata gttcttaact taatgtgtgc tgttgtttct
     3541 caaaagatgt atcctgatta tactcctgtt ggtgagttag agaaagaaac tattgacgat
     3601 attataaaaa aagtaaaagg attagttaca aacaaggtag gcggtacaat attacgatct
     3661 tcagattcaa ttattatttc atctttctta ggtctttcag tacttgcgat ttatcagaat
     3721 tattacttta ttatttctgc cataatgagt atatttgcaa taatttatga atcatgtata
     3781 gcaggtatag gtaacagtct tattgtagaa agtcaagaaa aaaattatat agatttttgt
     3841 gatattacaa caattatagg ttggtttctg gcaattagtt gtgcatgctt tattacatta
     3901 tttcagccgt tcatgattat ctgggtggga agtgataatc ttatgagtat ttcatttgtt
     3961 atatttatga tagttcattt tttcttatat gaaattgatc agttaattgg gttatacaaa
     4021 gatgctgctg gaatatggta cgcagacagg tttagaccat taattacagc aatgataaac
     4081 attgtattaa atatcatcct cgttaattgg ttcggattat atggagtttt ggcggcaacc
     4141 attgtttcag tattgttggt tgatttaccc tggttattat ataatgtttt ccaaaatata
     4201 tttaaatttg aatcggcagg taaatatgtt tggaagctca taagtttgtg tgtaatagct
     4261 tttgttgctt cttatttaag ttattttgct tgttcgtatg ttaatatcgg aaatttcaaa
     4321 gtgataagtt tagtgataaa tttagttata tcggttgtca tatcgatttt agttattctt
     4381 ctgtttaata tccgtaataa gaagtttaag tatattttga aagttcttac gtcggtattg
     4441 gtaaaaagat gagaagagac atcagaatga tatataaagt aactacaatt tttaggctgc
     4501 cgaataggcg aacgatgtat agtttttgtt ccaacgaaga ctcttgttga aattggagat
     4561 gatatgaagg agttacaatt ttgactcatg tttatgattg atcggtttta aaagtagtat
     4621 ggagaagctt tgggatcatc ggagaggatc aaaataggta ataatgtttt tataggaatg
     4681 aagtctagaa tactaaaggg tgtacatata ggaaataatg tagttatagg tgcaaatagt
     4741 ttagtaaaca aggacgtatc agacgattgt gttgttgcag gaaatctagc aaaagtaatt
     4801 atgtccctag atgaatatta taaaaagata aacaatatga agaatccgta aaacttgtga
     4861 aactataccg agaataatat gggaaaaata ttgatgaaaa agcattatat gagtttttag
     4921 ggctgtttac agatggagat gacaatttgt cgaagagttg ggagcaatgc aaaatttagt
     4981 aggggattat aaacagactg acaaggtatt taaagaacat gcacaagtat ttcataatat
     5041 tgaagaattt ttgaatactg tgagatagga gttgctacat tgagaaaagt atgtattttt
     5101 ccaatg
//
LOCUS       DAFGZE010000130         4853 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.134, whole genome shotgun sequence.
ACCESSION   DAFGZE010000130 DAFGZE010000000
VERSION     DAFGZE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.134"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(98..1246)
                     /gene="nagA"
                     /locus_tag="LTU54_002889"
     CDS             complement(98..1246)
                     /gene="nagA"
                     /locus_tag="LTU54_002889"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBL8297527.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1322..2158)
                     /locus_tag="LTU54_002890"
     CDS             complement(1322..2158)
                     /locus_tag="LTU54_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297528.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2342..3559)
                     /locus_tag="LTU54_002891"
     CDS             complement(2342..3559)
                     /locus_tag="LTU54_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBL8297529.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3708..3887)
                     /locus_tag="LTU54_002892"
     CDS             complement(3708..3887)
                     /locus_tag="LTU54_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297530.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3942..4025
                     /locus_tag="LTU54_002893"
     tRNA            3942..4025
                     /locus_tag="LTU54_002893"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3976..3978,aa:Leu,seq:gag)
     gene            complement(4265..4843)
                     /locus_tag="LTU54_002894"
     CDS             complement(4265..4843)
                     /locus_tag="LTU54_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297531.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1482 a         1006 c          780 g         1585 t
ORIGIN      
        1 acttttgaaa cttatgtaaa gtgattttta ctagaacaca aaaaaaacct taaaaccaaa
       61 cttaagaagt ttagttttaa ggttaactta aattatatta tgcgtggaaa cgttccacgc
      121 catctagata agttgcttcc aatgtcatat cagggttcaa tacgataaag tctgcatcgc
      181 gtccttgagc gatttttcca catgtatcat cgattttaca gctgattgct ggaatcagac
      241 tagccatcat gactgcttgt tctggtgttg ctaatcccca gtctacaaca tttttaattg
      301 cttctttcaa tttcaatata cttccagcta agttgccaga ttctagacgt gctgttcctt
      361 cttttactac tactggaaat tcgcctaaat tgtaatttcc atcaggcatc ccaccggcca
      421 tcatacagtc tgtaattaat gcgacatgat catgtcctgc tttttccatt aggattcccg
      481 cagcatttgg atgtacatga tggccatcac aaatcaattc agaaaagaca tgttctaaag
      541 ataataacgc acccaccata cctggttcgc gatgattcaa tccacgcata ccattgaaag
      601 catggacaaa tacacttgcg cctgcttcaa cagctttttg cgcttcttct agtgtagcat
      661 tactgtgtcc tagagaaaca actactcctt cttcagtcac ttgtttgaca aactcttcta
      721 caccttgacg ttccggtgct aaagcaattt ttttgattaa accacctgaa gcttcttgcc
      781 attcatggaa agtatccaaa tcaggatccc cgaaataact tgggttttgt gcccctttgt
      841 gttcttctgt gaagaaagga ccttcaaaat agattccttt gattttagct cctggtgctt
      901 cctgatacat tttgccgatt gtctcagcta catcttttaa acgttctttg cttgacgtca
      961 atgttgttgg taaaaatgaa gtcactccac aagataacag accttcagac atcactttga
     1021 tgccttctgc atcattatcc attacatcat gattcatata gccgtgaata tgtgtatcta
     1081 ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat gttgacttct ccctcaggta
     1141 gttctttggt gtattcacca aaaagtccat cggtaatttc aagatagcct gcatgtttga
     1201 cacctgacgc taaaaagaat ttgtcagcat agataaaagt tctcattcta ttctctccct
     1261 ttgtttttct acctctaaag tactcttatt tagagaagaa gtcaagaatg gtctacacca
     1321 tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa gccttcatag aatgctgctc
     1381 tttctggttc ttctttacgc ttgtatatta agcttttaag cacaaataaa tcattgaggt
     1441 aatagtagct attcttctct tgagcccaat agattccttg attcacctgt tctaacgctt
     1501 cgtcgatttc attttgatgg aataagaaat ttccataatt ataaaaaatt tgcgaaaatt
     1561 cactttttga tcgttcatca gctgattgat gcagcagttg gatgcttcga ctcaaatagt
     1621 agcgtgcttc agccgttttt cctgccacac catatatttt tccgatacaa ctaatcagtt
     1681 gtatttctgt gtcagaaaca tggattcttt ctttttggct agagtagctt aatgcttctt
     1741 tcaaataata aagggactga gagatatttt gacgcaacgt gaattcgcaa acaccgagat
     1801 aataataata gcgttgaaag tcagttgctt gatacagatt ttcagaaata tcagaagatt
     1861 ttagtacttg ttctaacttc cgataatcac gtttttggaa acaactatcc aacagttcaa
     1921 ataatttatc gtttgtccga gtagtttttc gagagagatt catcacatga tcaatcgtta
     1981 cccctagccg ttgacaaatc tgcatcatca ccaatacatt cggcagttcc tcattatttt
     2041 cgatccgact taatacactt tgtgaacaaa tatcatgcga aagcattttt tgcgtcattt
     2101 tttgttcttt tcgaatctct ttaatgatcg ttccaaagga ctcgattaac gtttccataa
     2161 gtatcctccc caaaatatgc gtattcgcat tctattcgta ttttacttta ggaagtcgca
     2221 aacttcaaga aaagacttgc aactaaataa tatactttag ttatttaaga aaactccact
     2281 agaaagagag cctcagtgtg tttgtctgtg gccctcctac tgaaaaattt ggtttttaaa
     2341 atcatttttg gcgataggtt gctaggaatt ctctcatttt cttagctgtt tgtttcaaat
     2401 catccatttt aggaagataa acgattcgaa aatgatctgg ctggttccaa ttaaatccac
     2461 caccatgtac aagtagaata tgatgttcat gtaaaaagtc caaaacgaat ttttcatcat
     2521 ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat ataaaaagca gcttttggtt
     2581 ttacggctga aagaccagga atatcattga ttgcattata gatatattct ctttgttcat
     2641 agatccttcc gcctggcagg agcagctcat cgacactttg ataaccgcct agcgcggttt
     2701 ggataatttg ctgagacaat acattggaac acaaacgcat ggaggacaac atattcagcc
     2761 cttctatata gcctttgacg tgtgacttat ctccacttag gaccatccag cctactcgaa
     2821 atcctgctac acgatgagat ttggatagac cgttcagcgt aacaacgaac aaatcaggcg
     2881 ctaatgtagc aatcggaata tgttctagac catccatcac taagcgatca tagatttcgt
     2941 cggaataaat aatcaggtta ttttgtcgag ctacttcaac gatttcttct aataattctt
     3001 ttggataaag tgccccagta ggattattag gattaatgat aacaatagct tttgtccgac
     3061 tcgttatttt tgatttgata tcatcaatat caggatacca ttctgcctct tcatcgcaaa
     3121 tataatgaac aggtgtgcct ccagctaatg caatagaagc cgtccacaac ggatagtccg
     3181 gcattggaac cagtacttca tctccattgt ccagcaaccc ctgcatacac atcgtgatca
     3241 attcgctcac tccgttgccg gtgtagatat cattgatcgt cacattcgga aaatgtttca
     3301 gttggcaata ttgctcaatt gcttttctag cagaaaagat ccccttggaa tctgagtagc
     3361 cttctgagtc acgggcattc atgatcaaat cacgaatgac ttcgtttggt gcttcaaatc
     3421 caaaaggtgc gggatttccc gtgttcaatt ttaaaatact aatgccctct tcttgcatcc
     3481 gctccgcttc ttctagaacg ggaccgcgaa catcgtaact gacaccttct aatttgttgg
     3541 atttttcaaa ttctctcatc acttagccaa tccctcattt caaaaagtta gaactttcca
     3601 aattttttgt tatttactat ttcaatatca tactccatcc tagaaaattt tgccacttat
     3661 ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt tgagaactta attgtcatga
     3721 tagtcattcc atagattcgc tgcccaatcc attgcaattc cacaaatgat tcctagcata
     3781 aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata cacctaagat cattcccagt
     3841 cctgaaaatt ctccgtcata tttctccatc ttaataagtc ctcccatttt tgagcttgac
     3901 aactatgtaa aacctcgcta tcattagtgt gttattatta cgcagatgtg ttggaattgg
     3961 cagacaagca tgattgaggg tcatgtgagc gttagctcgt gtgggttcga gtcccatcat
     4021 ctgcactaca actcgtgaag ttaattttgc ttcacgagtt tttttgtttg aacgtttctt
     4081 tatgccatta tatacatatc actgtatcct ttacaaaaag aacgtacaat catgtaaaca
     4141 gcctgaggtg agtatatgaa ggtcggaaac aagctactcg tagaaacttg caagtgttaa
     4201 aacaattaat cgaaaaacga gtaaaatccc tcacctaata ctaaagcgag ggattttttg
     4261 tatattatct gtttatcacc gttattataa aggtacaaat accaagcacg gttaatccaa
     4321 attgcataaa ttcatttttt tgtacactca tgcttttcat tagctcttta actgccatta
     4381 gtactaagat ggcgataaca acataaagta tatagtacgt ccgcattccg gaagcccata
     4441 ctgtgggaga aaatcccata attaaccttg aacaaaatcc cattatcatc aaaataacta
     4501 aaaaaataga agttttctta ttgtcaaaac ttaaataaat tccaacaatt aaacatagta
     4561 ataatgccaa gactactaaa gtagctatcc aagtacctgg ataaaaaagt gacagtttcg
     4621 ttcctaactc tgtaaagaga ttattcaggt tactactact ccaaatcatt gcccttttat
     4681 ttccattgac gtaagtaaag ctttgaccca ttgtattacc aaaataaata atcaggttta
     4741 agaaaaacgg taatgcagtc aatatacgca cataatagtt ttgacatttc ctatatgtta
     4801 gaaggaaaat taagaaaaat agcaataaaa ataagatatt catgtctaaa aaa
//
LOCUS       DAFGZE010000131         4826 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.135, whole genome shotgun sequence.
ACCESSION   DAFGZE010000131 DAFGZE010000000
VERSION     DAFGZE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.135"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            209..307
                     /locus_tag="LTU54_002895"
                     /pseudo
     CDS             209..307
                     /locus_tag="LTU54_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(990..1808)
                     /locus_tag="LTU54_002896"
     CDS             complement(990..1808)
                     /locus_tag="LTU54_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBL8297532.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1969..2658
                     /locus_tag="LTU54_002897"
     CDS             1969..2658
                     /locus_tag="LTU54_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBL8297533.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2672..4174
                     /locus_tag="LTU54_002898"
     CDS             2672..4174
                     /locus_tag="LTU54_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8297534.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4187..4663
                     /locus_tag="LTU54_002899"
     CDS             4187..4663
                     /locus_tag="LTU54_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8297535.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1614 a          704 c          861 g         1647 t
ORIGIN      
        1 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
       61 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      121 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
      181 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
      241 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
      301 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
      361 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
      421 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
      481 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
      541 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
      601 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
      661 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
      721 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
      781 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
      841 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
      901 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
      961 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1021 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1081 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1141 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     1201 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     1261 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     1321 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     1381 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     1441 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     1501 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     1561 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     1621 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     1681 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     1741 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     1801 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     1861 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     1921 tttggagttg ttatccaaaa ataataaaaa attggagtgt ttaggataat ggataaaaag
     1981 aggtttttag atcttatttc aaaaggtttg attgtttcct gtcaagcatt acctggtgaa
     2041 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2101 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     2161 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     2221 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     2281 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     2341 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     2401 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     2461 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     2521 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     2581 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     2641 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     2701 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     2761 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     2821 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     2881 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     2941 tggtggagta gcttatctag tatatactgc aactattagt gggtttcttg aacttttttc
     3001 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3061 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3121 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     3181 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     3241 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     3301 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     3361 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     3421 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     3481 gtttggatta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     3541 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     3601 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     3661 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     3721 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     3781 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     3841 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     3901 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     3961 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4021 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4081 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4141 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     4201 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     4261 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     4321 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     4381 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     4441 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     4501 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     4561 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     4621 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     4681 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     4741 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     4801 gtaatttaaa gttataaaag cttcga
//
LOCUS       DAFGZE010000132         4769 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.136, whole genome shotgun sequence.
ACCESSION   DAFGZE010000132 DAFGZE010000000
VERSION     DAFGZE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.136"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(205..1167)
                     /locus_tag="LTU54_002900"
     CDS             complement(205..1167)
                     /locus_tag="LTU54_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8297536.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1169..2608)
                     /locus_tag="LTU54_002901"
     CDS             complement(1169..2608)
                     /locus_tag="LTU54_002901"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBL8297537.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2793..4739
                     /locus_tag="LTU54_002902"
     CDS             2793..4739
                     /locus_tag="LTU54_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HBL8297538.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1518 a          816 c          787 g         1648 t
ORIGIN      
        1 tccaatttcg taaaacaacc gtaacagttg tgtaaaaata tcattttaga gcatgaaaaa
       61 aggagtggat ttctccactc caaaaaagtc taacttttgg gggtcactac aagttgacaa
      121 ctcctttttg tttacaaatc aattggatat ctacaaattg tattttctaa cgaattttat
      181 agacattaaa aaattaattt aacgttaaat acttcctcca gaaagtattg agattggtaa
      241 aatataatcc ttatttgtct tttcgccttt aatttttttc agaagaatat caacaataag
      301 acttgcaatt tcatcaattg gttgtcttat tgttgtcaat tgtgggaaaa atttctcaat
      361 aaaagaggta ccatcgtatc caataatttt taaatcttct gggatattaa gtttaagttg
      421 atgagcaatt ttcatagtga gaatggctgt taaatcatca gaaacaaaaa taccatctgg
      481 tttgtgattt ataatagttg actttatttc catttcacga cggatagtag acagattatt
      541 aggaattttg aaaatttttc catttggatt ttgaaaagaa aaacctagtg cacgtaattc
      601 tgtgggagaa tccgtgttat catttcctgt aatcataatg gtattttgac aaccattttt
      661 ttgtaaagtc ttagctgcaa gttttccccc ttcaaagtta tctgaagaaa caatggaaac
      721 attaggagct aaatttctat caaaagcaac aataggtgct ctcacacgct cataatcagc
      781 aattccaaga ttatggcttg atgagattat gccatcaact tggttggctt caagcatttc
      841 aatgtattct ctctctttag caggatcatt ttgactattg caaataatcg ttttatatcc
      901 atgtttaaat aactctattt cgagatactc aatgagttca gagtaaaaga tattactaat
      961 attaggaaaa ataagaccaa taagctgagg tgactttcct tgaagtccac gtgctaaatt
     1021 atttggttta taaccaagag ttttcatagc ttcatgaact ttttgttttg ttttttctga
     1081 taaatatccc ttattattaa tgacgcgtga aacggttgtt gggctaactc cagctaattc
     1141 agcgacatca gttaattttg ctaccatttt aatattttaa ttcaaagtat tttccagtag
     1201 gttcgcctga tttgatttga ataccatttt ggtgattttc agggaataca cgactagtaa
     1261 acactttttc tcctttatta ataaaaatct caatgattga gttatctaca aaaatgttag
     1321 cagttgtttc tttagtatca attgagcatt cacgaatcgt accaaagtca gtggcatatt
     1381 gaatgcctgc tttgctgcga tctaaaataa tttttccttc tttggtatct acaatcaagc
     1441 taagtccatt tcctttacta tcagagaaaa gaagtaactc cgatttctga tttggaggaa
     1501 aagttagttc aagctcataa gtattatttg tttctgcttt tgctgtaaat tgttcagaac
     1561 tagcacgtaa tgatgtaata gcttctactg gatattggta aagtttacca tcttttattg
     1621 aaagttcttt tacaagactc attgctccct gataatcata cttatcagtt ggataatcga
     1681 tatcaggtag tccaatccaa ctgacaatca gagcacgacc atcaggagta ttgaatcctt
     1741 gagtcgcata cgcttcaaat ccataatcaa gattttgaat ttctgaagcg cccacaagtt
     1801 ttactttttc tatgtcaagc gattgacaga tcttaaaagt attcggatag atattcccat
     1861 aatttagctc agatttatcc aacccttgag gacaataaat gagaacaggt tttttgtcta
     1921 caaatactag atttggacac tcaatcatgt attctgaacc agtgccgcca aaatcaaggt
     1981 tgccaacttc ctcccatgtt tccacgttat tgtcatccgc tttgtaaagt ttgataaagc
     2041 ctgatttttc taaattttgt gctccaacaa tcgcatacaa ttgtccttca taattaaaca
     2101 tttgtggatc acgaaaatga tcagttacat cagcaggttt atgaataaga acattctcac
     2161 ctttcgtaat gttgtagtct ttatccatcc atgctccgat ttgaagtgta tcacgttccc
     2221 aattttcatt tcgaacgttt cctgtataga gtaagaatag attcccctct atttcatatg
     2281 cagaaccgga ataagcgccg tggctatctc gttttgtgtc tggaagaagt tctgctcctg
     2341 ttacatgaaa atgaactaaa tcatctgatt cattgtgtac ccattctttc aacccatgag
     2401 cagctccaaa tggccaattt tgataaaaaa gcgtatattt tccgttaaaa aaagaaaaac
     2461 cattaggatc gtttaacaat cctgttttag gttcgatatg atagctagca cgccaaggtg
     2521 atcgttttac attatcctta attttatcca tctcatcttt agtccaatca gaataagcac
     2581 gatatcgtat ttctcgaggt aagttcatat tttactcctt caatttatct ttttacaact
     2641 agtatggtaa acgttttccg ttgaaaaatc aatcatttca tttaaaaaaa taaactatat
     2701 gacaaacgtt tgacatattt ttaagatatg ctaaaataaa tttgcatttc agtgaaccgt
     2761 tttcacgtaa aaataaaaaa ggagtgattc gaatgaatta cagtaaagta gcttccgacg
     2821 taatacaagc ggtcggaaaa gataacctta ttgcagctgc acactgcgct acacgtcttc
     2881 gtctcgtact aaaagatgac acgaaagtga atcaaaaagc tttagacgaa aatccagatg
     2941 tgaaaagcac atttaaaatt aatggacaat atcaagtgat tatcggtgca ggtgatgtta
     3001 atttcgttta tgacgaactt atcaaaaaaa ctggactttc ggaaatttca acagatgatt
     3061 taaaacaaat tgctagtaaa gataaaaaat tcaaccccat tatggcatta attaaattat
     3121 tatcagatat ttttgttcca attatacctg cccttgttgc aggtggttta ttaatggcat
     3181 tacaaaactt tttaacagct gctggacttt ttggaccaaa atcaattgaa gaaatgtatc
     3241 ctgcgattga aggtatttcc tcaatgatcc aactgatgtc cgctgcacca ttcatgttct
     3301 taccaatttt agtaggtatt tcagctgcta aacgttttgg agcaaaccaa tttcttggtg
     3361 ctgcaattgg tatgatcatg acaacaccag atcttggtgg ctcaactgaa tattggaata
     3421 tttttggtta tcatgtttca caaacaaact atgcttatca agttattcca gttttagcag
     3481 ctgtttggat attatctata ttagaaaaat tctttcataa aaaattgcca tcatctattg
     3541 actttacatt cacgccatta ttatcagtaa tgattactgg atttttgact ttcacagtta
     3601 ttggccctgt aatgttattg ttgtctaatg gaattacaga cattattgtt tggctatata
     3661 acactactgg atttattgga atgggtattt ttggaggaac atattcactt atagtcatga
     3721 ctggtttaca tcaatcattt ccagctattg aaactcaatt actttcagcg tggacaaatg
     3781 gtatcggaca tggagacttt atctttgtag ttgcatctat ggcaaacgtt gctcaaggtg
     3841 ctgcaacatt tgccatttgg ttcttaacta aaaattctaa gacaaaaagt cttgcttcct
     3901 ctgcgggtgt ctcagcactt cttggaatta cagagcctgc cttgttcgga gttaacttaa
     3961 aataccgctt tccattcttt tgtgctttaa ttggttcagg aattgctaca gcagtagcag
     4021 gattacttaa agtagttgct gtttcacttg gttcagcagg tttcttggga tttctttcaa
     4081 ttaatgcaac atctattcca ttttacgtat tgtgtgaatt gattagcttt gtagttgctt
     4141 ttgcaattac ttacttctac ggtaaaacaa aagctgctgg tatctttaaa gcagaagaaa
     4201 ttgctgaaca aacaccagtt aattcttcaa aagtagcaac aacagatcca atatctactg
     4261 atgttgaatc aacacaaaca aaagtttcat cagaaacaat tgttagtccc atttctggta
     4321 aagtgattag tctttcttca gtaaatgatc cagtcttttc atcagaagct atgggaaaag
     4381 ggattgccat taaaccaaat ggaaacacgg tccattctcc agtagatggt actattcaac
     4441 tcacgtttga aactggacat gcttatggta ttaaatcaaa taatggtgct gaagttctaa
     4501 ttcatattgg tattgatact gtatcaatgg gaggaaaagg atttacacaa aaagttacta
     4561 ctaatcaaaa agttagaaaa ggcgatattc ttggagcatt tgaccgttca atgattgaga
     4621 aagaaggtct tgatgataca acaatgatta ttgtgaccaa tacttcggat tattcagaag
     4681 taaatatagt agctgaaaat acagtcactg agggtactga tttacttgaa atcaaataaa
     4741 tatatttccc tgagtaaaga tggaatttt
//
LOCUS       DAFGZE010000133         4666 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.137, whole genome shotgun sequence.
ACCESSION   DAFGZE010000133 DAFGZE010000000
VERSION     DAFGZE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.137"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(448..978)
                     /locus_tag="LTU54_002903"
     CDS             complement(448..978)
                     /locus_tag="LTU54_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HBL8297539.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMVDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            1083..1633
                     /locus_tag="LTU54_002904"
                     /pseudo
     CDS             1083..1633
                     /locus_tag="LTU54_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
     gene            complement(1786..2259)
                     /locus_tag="LTU54_002905"
     CDS             complement(1786..2259)
                     /locus_tag="LTU54_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBL8297540.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            2397..3869
                     /locus_tag="LTU54_002906"
     CDS             2397..3869
                     /locus_tag="LTU54_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBL8297541.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            3866..4576
                     /locus_tag="LTU54_002907"
     CDS             3866..4576
                     /locus_tag="LTU54_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HBL8297542.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         1465 a          847 c          895 g         1459 t
ORIGIN      
        1 tttttgctgg gagcattatt tcattttgaa gtcactaatt taactgtgtg aatatgcagt
       61 tgttacaatc tttgataaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac
      121 ggcaaaatat gaggaactat ttttccattt gtttctctaa tactcaggtc aaaacgctga
      181 tttcacaacc tcttgataaa cggtgttcaa acggctgctt ctcggtaata agcccactag
      241 cctagaaaat atgaaaagct attttctaac tagcgttctt attactcaaa gcataacgct
      301 gatttcacaa cctcttgata aacggtgttc aatcagctga ccctcggtat tagctcaaaa
      361 aacggcaaaa catgaggaac tatttttccg tttctttctc taatactcag gtcagaacgc
      421 tgatttcaca acctctcttt cacaacctta ttttactcgt ttataatttt catcgaagta
      481 ttttccgcta cggatatcat ctggcattcc atcgtatggt tcttcgctaa taacatcttc
      541 attgtctata cctgcatcaa ccatataagt gatcgtagca aattcaggaa caagtaattt
      601 tggataagtt tcatattttt cacgagctgc ttccatttct gcgatatgtt cgttttggaa
      661 agtaacagtg atttctggat gttcttcaac ccatttaggg aaaaagatca ttccgtgaag
      721 tttcttttca tttggcataa agtccaatgc cacgtctgtc cctgttggct ctgtccaagt
      781 gattttgaat acgccgtccg tcaatttaac gatatcagct tcttggtctt ttacccaacg
      841 gcctttgacc attccgccat ggatacggta atcaacagta tggtcatttt tggcatacca
      901 ttcatattcc caaccattgt catacgtata gataaagtga gttccaataa aatcttccaa
      961 ttctttaaat tctttcatgt ttaagcatct ccttataaat taaaacatgt aataaactac
     1021 atgttttcga ttactcattt atgatagaat ggatttaaag atttgtcaag gagggaaaac
     1081 tgatggcaag aaaaaataca cttcgatata ttttgttagg gttattaagt aagaagaaga
     1141 tgacaggata tgaattgaat caatcgttca aaaatgaaat aggagaattt tggcaagcaa
     1201 aacatagtca gatttatcct gaacttgcaa aaatggaaga gcaaggaatc attcagcatc
     1261 aagtagagat cacaggtgaa aaattggaaa agaaagttta tgaattaaca gaagaaggga
     1321 aggaacaatt ggcagagtgg attcatacac ccaccaatga actaccggtt aatcgagacg
     1381 aatttgttct gaaattgtat ttcgtcaaag acgtgaatga tccggcatta gtagaaatta
     1441 ttcagcagca gcgtgacctt catgaagaaa aacgacttca tctattagag cgaaaaaaac
     1501 gatctttcct actgaaaaag aaaaacatga aaattacgga cactacctga ttttagcgca
     1561 tgcgatcaat cgggaaacag aatatgcaaa ttggctaaca gatgtattag aagaaagtaa
     1621 ccgaaaaaaa taaaaaaata tccgaacagt gatggagaat tcctttctat atgaaagaaa
     1681 cgcagatcac cgttcggata ttcttatgtt caaacaggcc ttttttcaag tatttatgta
     1741 gcatctactt attaaaaagc ttggtactta ggagattagt ggcgtttaga gttcttttaa
     1801 caactgttcg tagtcagctt tttcatcgac aggaaggagc ttgtaagtta attcgtctgt
     1861 ctcgttgtca tgaatgatgt acatcttagc attctcttct ttgaagttac cgatttgtaa
     1921 aatcgtatag cgttcgtcga tcaattcgat atctttgatt gcttttgaaa aaactttacg
     1981 gacatggtgc attttctttt ggcggatttc tgccttttct gcagcttttg acaaagtaaa
     2041 tccgtcatct aaatattttt tgatcagttc tacttttatg actgtaggta aacgatattt
     2101 tctaggacaa ttcgcatcag cttcgatcga acgaatgaat cccttttgtt cccaatacct
     2161 taattgacgt ggagacacac ctacgatatc acttaattca ctgatgccaa caaccaattg
     2221 atcattttcc aacaaacgtt taagggaagc cccaaccatt tttgtcactc cttttgtcct
     2281 cttgcttccc ttaattatat ccgtattaac ttatgtgtca agtaggttga caaatagcga
     2341 atttaggact aaagtattct agtagtttca gaaaaaattc agaaagaagg agaaatatga
     2401 gtaacaacca acccgtagat atttacggaa aaccatataa tcggacactt ttagttgttg
     2461 tcttattaat tggtacattt tgtaccgttt tgaatcagac cttattgacg acagcttttc
     2521 ctacattgat gaaagctttc gatatttcag cgtctgatgt gcaatggtta acgactggtt
     2581 tcttgctagt caacgggatc atgatcccaa taacagcatg gctgatcaat aaattcagtt
     2641 caagaaattt atatctttct gccatgacga tctttttgat cggaacgatt acttgcttta
     2701 cagcacctaa ttttagtaca ttactaatcg gtcgtttgat ccaagcttgc ggtgtgggag
     2761 tatcgatgcc attattgcaa aatatcatgc tatctatttt cccgccagaa aaaagaggat
     2821 cagcaatggg gatgtcaggg atcgttattg gacttgctcc tgctttaggc cctacattat
     2881 ctggttatat cattgataac taccattggc gtgacttgtt tggtatggta ctgccaatcg
     2941 ttgttcttgt cttggtttta gcattcttcc taatgaaaag tgttattcca ttgtcaaatc
     3001 ctagcattga tattttatcc gctattttat caacgattgg attcggtagt ttactttacg
     3061 gattttctag tgtaggagat gacggctggg gaagcttgaa agtaattggt ttcttagttg
     3121 ttggtgtgat tttcatcgcc ttgttctcat ggcgtcaatt acatttggaa catccattct
     3181 tggaacttcg tgtattcaaa tcatctacat tcacgattgc agctgtttta gctggtgtaa
     3241 caaatatggc gatggtaggg gcagaaatgg tcgtaccatt gtatattcaa aatattcgtg
     3301 gagaatcagc attccattct ggcttgatgt tgttgccggg tgcattgatc atgggggcta
     3361 tgatgcctgt gacgggtgcg attttcgata aatacggcgc gaaacgttta gccatcacag
     3421 ggatgttcat tttgacagcg gcaactgcac catttgcttt cttgacaaaa acgactccag
     3481 ttttatatat cgtgatcctt tatgcgatcc gtatgtttgg gatctccatg gtcatgatgc
     3541 cggtaacgac ttctggtatg aatgcattgc caaataactt gatcagtcat gggacagcgg
     3601 taaataatac attccgtcaa atcgcaagct cgattggtac agctattttg atcagtgtgt
     3661 tgacgaatgt gacaaaagca aatatcccaa gcaaatctgt attggataat acgccattag
     3721 cttataaaga tcaggcaatc aatgccacac tgtcaggtta ccatgccgcg tttatcgtag
     3781 cgatcatctt tgggattctt ggctttatta ttgccttttt attgaagaaa aaagaacctg
     3841 ttctaatgaa agcgggggat aatgcatgat tattggagtt ttgattgtca gcttgcttgc
     3901 ttttgcttta acgaacgtgt tcgctaaaaa aacatggcaa acctttttat ctttgatttt
     3961 cggactaatc ttcgtcgctt cactaagttt gatcgtcgct aatttgtcca atcactttgg
     4021 aatggaaaaa gtaacggaaa caaaaacaga aaaaattgtt tccagtgctg attcccaagg
     4081 agcagatatg cttttgtata aagctttagg gaatggaaaa gaaaaagtgt atctttaccg
     4141 aacaaatgaa aaacaagaaa aaccaaaagc aactggtacg gataacgaaa caaataaagt
     4201 agaaaagaca gacggcgatg ctgaaaaagt gacgaaaact acttattggg aatacaaaaa
     4261 cgacatgtat aaattctggt tcaatattgc tgataacaat cacgaatatg acagccgagt
     4321 gaatacattc aaaatcccag agacatgggt agaattaagt acggaccaag cagccaagtt
     4381 agctgaatta gtgaagaaac agcaaagtac gatggaaagt gaagcaaaag cttatgttca
     4441 agacggtatg gtaaaagcaa tgactgaaaa tccaaaaatg agcaaagcag agcaagaaca
     4501 acgtacgaaa gccttagctg ctgaattcca acaacaagca ttcgcaaaat tagtgaaaga
     4561 agcaaaaggc gaataatatt ttgaacacca tgtaggaggt tgtgaaaaag ctgctctgca
     4621 tcaagcagta agaacaatca gataaaaata gcactccata tttttc
//
LOCUS       DAFGZE010000134         4466 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.138, whole genome shotgun sequence.
ACCESSION   DAFGZE010000134 DAFGZE010000000
VERSION     DAFGZE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.138"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            107..358
                     /locus_tag="LTU54_002908"
                     /pseudo
     CDS             107..358
                     /locus_tag="LTU54_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            451..1245
                     /locus_tag="LTU54_002909"
                     /note="aph(3')-IIIa"
     CDS             451..1245
                     /locus_tag="LTU54_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL8297543.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1655..1738
                     /locus_tag="LTU54_002910"
                     /pseudo
     CDS             1655..1738
                     /locus_tag="LTU54_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1787..1870
                     /locus_tag="LTU54_002911"
     CDS             1787..1870
                     /locus_tag="LTU54_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBL8297544.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1995..2732
                     /gene="erm(B)"
                     /locus_tag="LTU54_002912"
     CDS             1995..2732
                     /gene="erm(B)"
                     /locus_tag="LTU54_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL8297545.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2995..3858)
                     /locus_tag="LTU54_002913"
     CDS             complement(2995..3858)
                     /locus_tag="LTU54_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HBL8297546.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3860..4132)
                     /locus_tag="LTU54_002914"
     CDS             complement(3860..4132)
                     /locus_tag="LTU54_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBL8297547.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4149..4358)
                     /locus_tag="LTU54_002915"
     CDS             complement(4149..4358)
                     /locus_tag="LTU54_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HBL8297548.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT         1376 a          751 c          830 g         1509 t
ORIGIN      
        1 tttcttggga gttttgagac tactatatgc gtttttttgt tgatttacca acatgtttag
       61 acattttcct ctaggaaaaa attaactagc accacgggtt atcttttata tagaagatat
      121 cgccgtatgt aaggatttca gggggcaagg cataggcagc gcgcttatca atatatctat
      181 agaatgggca aagcataaaa acttgcatgg actaatgctt gaaacccagg acaataacct
      241 tatagcttgt aaattctatc ataattgtgg tttcaaaatc ggctccgtcg atactatgtt
      301 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
      361 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
      421 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
      481 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
      541 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
      601 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
      661 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
      721 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
      781 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
      841 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
      901 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
      961 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     1021 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     1081 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     1141 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     1201 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agatttagat
     1261 gtctaaaaag ctttaactac aagcttttta gacatctaat cttttctgaa gtacatccgc
     1321 aactgtccat actctgatgt tttatatctt ttctaaaagt tcgctagata ggggtcccga
     1381 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gttattttgt
     1441 acatttaact tgacggtgac atctctctat tgtgagttat tagtggtaca gttttcaacc
     1501 gttttaatta taaaaaagtg gtgcattttt aaattggcac aaacaggtaa cggttattgc
     1561 aggtgtattt cttatctatg ggtttaacat ggattttatc attaaaatca tgagtattgt
     1621 ccgagagtga ttggtcttgc gtatggttaa ccctaaagtt atggaaataa gacttagaag
     1681 caaacttaag agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag
     1741 ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga ttcgtcatgt tggtattcca
     1801 aatgcgttat gtagataaaa catctactgt tttgaaacag actaaaaaca gtgattacgc
     1861 agataaataa atacgttaga ttaattccta ccagtgacta atcttatgac tttttaaaca
     1921 gataactaaa attacaaaca aatcgtttaa cttctgtatt tgtttataga tgtaatcact
     1981 tcaggagtga taacatgaac aaaaatataa aatattctca aaacttttta acgagtgaaa
     2041 aagtactcaa ccaaataata aaacaattga atttaaaaga aaccgatacc gtttacgaaa
     2101 ttggaacagg taaagggcat ttaacgacga aactggctaa aataagtaaa caggtaacgt
     2161 ctattgaatt agacagtcat ctattcaact tatcgtcaga aaaattaaaa ctgaatactc
     2221 gtgtcacttt aattcaccaa gatattctac agtttcaatt ccctaacaaa cagaggtata
     2281 aaattgttgg gaatattcct taccatttaa gcacacaaat tattaaaaaa gtggtttttg
     2341 aaagccgtgc gtctgacatc tatctgattg ttgaagaagg attctacaag cgtaccttgg
     2401 atattcaccg aacactaggg ttgctcttgc acactcaagt ctcgattcag caattgctta
     2461 agctgccagc ggaatgcttt catcctaaac caaaagtaaa cagtgtctta ataaaactta
     2521 cccgccatac cacagatgtt ccagataaat attggaagct atatacgtac tttgtttcaa
     2581 aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa tcagtttcat caagcaatga
     2641 aacacgccaa agtaaacaat ttaagtaccg ttacttatga gcaagtattg tctattttta
     2701 atagttatct attatttaac gggaggaaat aattctatga gtcgcttttg taaatttgga
     2761 aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca cctctaataa
     2821 ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt ttaaatctat
     2881 ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga aatgttttta
     2941 taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta aggtttaaat
     3001 acctggaagt ttaggtgttt tgggtattgg tggtgtaggt ggctgcaagc tttccaattt
     3061 ttgttgaatt gctttaaatt caggtgtctc ttggtgttta tttagaacca ttttttgctc
     3121 tattcgttct aaagtcggtt gaatttcttt ccctgatact ttacgattca attctttttc
     3181 taaggtttct tttggactaa tagaaggtgt ttccaaactt gaatagagtt ttactccttc
     3241 tctgttatat agccttatat cgctaaataa gcccgtttta tgaagggttt ctaaattggt
     3301 cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctgg ctgtcattgg
     3361 atcatctgca tacatggttt catatcgttc aattgttcct aaatatgagt tgattttagg
     3421 tactgccatg acatacattt ttgtttcata acctttggct tgaagcattg ttgcggtttg
     3481 aataggaacg tctgttgttc gtcctgtacc ttcgatcacc aaattatacc cttgatcgct
     3541 caaacggctt atgatcgctt ctgtcatgcg attagaataa ggggtaacgt gttttactac
     3601 gtctttttca taaagtttca ctagttcatc aaaattaggg tgctgttgtt taaaggtatc
     3661 attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc gcaaactggt
     3721 tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa ccgctttttt
     3781 tccttgaatc aattcttcta aattatcatt taagcgattc tcaaattgtt tgtcagtaaa
     3841 attgactata tttgccatat taagccactt tctctttatt caaaacttgt tttgagtaac
     3901 gattcattga ccgccaatac tcatgaacgg cttgtaattc ttctaaagaa taatcaaaaa
     3961 gatttacacg ttttgcaagc tttaattggt ttaataaatt gacttccaat aattgagagt
     4021 catttttatt taattcatgg ttcaaaacat agtttaatac tcgattataa acgcttgccg
     4081 ataattcgtt aatgatctct atttcaaatg ttttttcata cgtaactgcc atttcatttt
     4141 cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa ggcttgatcc
     4201 ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat atggtgcaag
     4261 tcagcacgaa cacgaaccgt cttatctccc attatatctt tttttgcact gattggtgta
     4321 tcatttcgtt ttgctttttg tgcgcctaaa tttcccacaa tcactcactt ctttctattt
     4381 cttcttactc ttattttatc atcaacaatc acaaatcaca agtgatttgt gattaatcac
     4441 ttgtgatttg tgattctatt cttttt
//
LOCUS       DAFGZE010000135         4434 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.139, whole genome shotgun sequence.
ACCESSION   DAFGZE010000135 DAFGZE010000000
VERSION     DAFGZE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.139"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(543..2048)
                     /locus_tag="LTU54_002916"
     CDS             complement(543..2048)
                     /locus_tag="LTU54_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBL8297549.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDN
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="LTU54_002917"
     CDS             complement(2045..2731)
                     /locus_tag="LTU54_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8297550.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LTU54_002918"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="LTU54_002918"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBL8297551.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1240 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgcat tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAFGZE010000136         4372 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.140, whole genome shotgun sequence.
ACCESSION   DAFGZE010000136 DAFGZE010000000
VERSION     DAFGZE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.140"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(83..1051)
                     /locus_tag="LTU54_002919"
     CDS             complement(83..1051)
                     /locus_tag="LTU54_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBL8297552.1"
                     /translation="MSKKRTIVNEWEKLTALSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRSDVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1496..2902)
                     /locus_tag="LTU54_002920"
     CDS             complement(1496..2902)
                     /locus_tag="LTU54_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBL8297553.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDEN
                     IYLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     ILDDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDE
                     ISPHLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIE
                     RLMNDYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYIYRQREE
                     NERDKALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSENEIEW"
     gene            complement(3124..3486)
                     /locus_tag="LTU54_002921"
     CDS             complement(3124..3486)
                     /locus_tag="LTU54_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297554.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDDLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3490..3828)
                     /locus_tag="LTU54_002922"
     CDS             complement(3490..3828)
                     /locus_tag="LTU54_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297555.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3807..4226)
                     /locus_tag="LTU54_002923"
     CDS             complement(3807..4226)
                     /locus_tag="LTU54_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297556.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4219..4359)
                     /locus_tag="LTU54_002924"
     CDS             complement(4219..4359)
                     /locus_tag="LTU54_002924"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297557.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1242 a          978 c          604 g         1548 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgatcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga cagggctgtt aatttttccc
     1021 actcattcac gattgttctt tttttactca tattcagttc ctcctcaaag aaaaaagaga
     1081 tttttggaaa gaatttccag agttcacgta actctaaaaa ttcataacca tcaatctcta
     1141 gtcttgaaga aacctaaaaa tattcgtata atgaatttga gttacggaac tagtgtggtt
     1201 agtttccgta aaggtcgaga acgttggtag cgttgctcga cttatttttt tgctcttttt
     1261 ccagttcatt tgtttgctaa ctcaataata gcacttctct ttagctatgc aaataccaaa
     1321 aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc taagttggcc
     1381 aaaaaatgga cttgccattt tttagcaaat gtcagcttca ggggctagtg gtttagccgt
     1441 caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat cgtggctacc
     1501 actcaatctc attctcagaa cgctctaatt tatgctgtga gacgttttga cgataaaata
     1561 aggttttctc ctgctctaat acttctaatt cctcagaaag ccccatatcc ttttctgcaa
     1621 gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt aatcgctcta
     1681 aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact tgtaaatctg
     1741 ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca tactcattta
     1801 attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca gcatttctct
     1861 tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc ccagataaat
     1921 gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aaccatatct ttttggaaaa
     1981 tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca atcttcgctc
     2041 tctcagaagc catcagagcc ttatcacgct cgttttcttc tctttgccta taaatatacc
     2101 tcaactcatc agaaacgctc tcacgctctt ttacagccgt ttggcgcaat tcagaaattt
     2161 cactcatgat ttctttgtat tcgtgaatat ctttaatttt gttggtaccg acaatatccc
     2221 ttttccaacc gtaatcattc attaatcgtt ctatagagtc aacttcttga tttctaaaat
     2281 tattgaaaag cactcgacca ttatctttat cagcgtgtat cccttgttga accagtgctt
     2341 tattaaagct cggctgcttt tgaacacccc gtttataacc ttcagcaacc ggcacaatat
     2401 tcaagtgcag gtgcggactt atttcgtcat tatgaataac agcattataa acagccaaat
     2461 tagggtttct ctcttgaaac tccttgtaat actgaaccaa aattttattg gcaatcactc
     2521 gattttgttc agcttgttgt ttcgatatgc cagtagaaga accatctgct cgatttgtct
     2581 ggaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc aactttttat
     2641 ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt tgtttcgcat
     2701 tatatttttc taccacttcg ccaaacaatt cctcatattt atctgctaga ttcgtctgct
     2761 ctaaataaat attttcatcc gatttcgtga agtcaatgtg cttatgccaa tcattggttt
     2821 tctggcgatc agttaactca cgattattat gagaaatact tgtattcttc gttccttttt
     2881 taaatgaaat actaaacccc aagttaaaac cctccttcgc gctgtcgcta ataaaaagat
     2941 tgtaaacctt tctgttactc tttgtcaaag agtaacattt tcctaattgt gacatggatg
     3001 tcacaattcc aaacgctctc cgagggtttt cgctgtcaca agtgacaacg aaaaaaggag
     3061 ccgaaagcgt atcacgctat caactccttt tatgctttat tttgcttcac gcaaaataat
     3121 cgcttatcgt aaattcacgt cattaaatat tgacgtaaat ttactctcac tcatctgact
     3181 tttcttattc ggtatcactc cgaataagtc gtcagattca tcgggataat ctcgttcgct
     3241 tggcgctgat ttttcatcag caagccactc attatccact gttttttcgt gttgctgatc
     3301 gtcagaaatt gacgataacc accgctcaaa atcagcaaaa gtttcaaacc caaaacgctt
     3361 gcgaagagcc ttgcccattg cagcattttc tttctcaatc aagcgttttt tctgttggcg
     3421 aagtttcgct tgttcttttt ttaatttttc ttcttgcgct ttaatatctg ctaaagtcat
     3481 tcccataaac tacatctcct tttctttgga attaataacg gaaatttggt ttaactgcat
     3541 aacatgactc attaatttca accaaaattc tttttctgat tcatcagttg tttgcgctaa
     3601 atgcttatga acaaattcat tagtcaaaac tgaagatttt gctagatcag cattgataac
     3661 cgagttgaga acatccataa ttcgttcaag catttcacta taattatctt catccggcaa
     3721 attcaaataa tcagctaacc gttcatacat caattcaatt ctatcgactg tttcttctct
     3781 caaatcaatt ttatccatgt cacacctcac ttccagaatt gccaccattt tcgatttttt
     3841 aattcttgat tttctagtaa caactgatcg tttttttgtt gctgaagcaa agtcaatttt
     3901 tgttggtcac gaatcatttc cgttaattca gaaatatgcc tatctttttc cgctaattgt
     3961 tcacgcaaaa attcattttc tttttctaac gggaaatcat tgcctgcatc attgaccggt
     4021 aaatcaccag tcaattgacc accttttgac cgataaattg accgcacaac accaatcaat
     4081 tccttgtcta acatgataac cccattatct ttataccgag caaggtcata acctgcattg
     4141 accagttgat tgcgcactgt atttttagat atacccaact catcagcaag gttttttatg
     4201 gtcatttttt ctggcaaatc agtcattatc ttctccgtta ctttcagcta ccccataaac
     4261 atatttaaca gcatgcttag ggataatgat tggggaatca attttattat ttttcaaaat
     4321 caactcatac tgcgttacat caagtaactt accggacatt tttttgctat cc
//
LOCUS       DAFGZE010000137         4221 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.141, whole genome shotgun sequence.
ACCESSION   DAFGZE010000137 DAFGZE010000000
VERSION     DAFGZE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.141"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            75..503
                     /locus_tag="LTU54_002925"
     CDS             75..503
                     /locus_tag="LTU54_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8297558.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
     gene            490..732
                     /locus_tag="LTU54_002926"
     CDS             490..732
                     /locus_tag="LTU54_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297559.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(1032..1247)
                     /locus_tag="LTU54_002927"
     CDS             complement(1032..1247)
                     /locus_tag="LTU54_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297560.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            1354..1668
                     /locus_tag="LTU54_002928"
     CDS             1354..1668
                     /locus_tag="LTU54_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297561.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1772..2950
                     /locus_tag="LTU54_002929"
     CDS             1772..2950
                     /locus_tag="LTU54_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8297562.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            3353..3646
                     /locus_tag="LTU54_002930"
     CDS             3353..3646
                     /locus_tag="LTU54_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBL8297563.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         1446 a          630 c          821 g         1324 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga aagtatttgg
      481 gtgtcaaata tgaagcaaaa taaacgatat ataccattca taccactaac gacaatcaaa
      541 gccgcagctt ctggtgattc aattgccatg tccatcgttc tgtcaaaata tcaaaattat
      601 atttcacggt tagccatgaa accagtttat gaagaagaaa aaggaatggt tatgtatgta
      661 gatgaatata tgcgtagaca actggaaaca aaattgattg agaagatact tgaatttgat
      721 acaactcgtt aaagtatttt ttccctttcc ttttcattct atggaagttt tttatcgggt
      781 caaaataaag gatttccata ggctgaaaat agcctagcgc tctttgacaa atgaatagca
      841 aatctatcag tacatgtaga ttttgattct ctagttacgc catgacaagg ttagctttta
      901 agcgacaaat tttaatatga gaatcgcgac tacgataatg atacttccgt aaccaaatcg
      961 gcccgccatt actgggggga ggtgagattc ctatgtgctt tggcaaaaca gctgatagat
     1021 cagaaaaact actatttcca tatttcgatt ttggttggca tatttttcat gccaaattct
     1081 gtgattttaa tagcatcggc aagatttaat ttttcttcag ctctctctcc acttttaagt
     1141 ttttttactg tactcaagtt gattcctgtt ccgaatgcta ttgcattagc cgtagatttt
     1201 tcaagaattt ctttaattat ttttatgtct gcaattcctt gtttcatatt aaaaattcct
     1261 cttttctagt ccgttttgat ggagtactgg agtgtttttt tacaatttta aggggtaccg
     1321 tggtggtcgt tttgattttg agagagaagg gtgatggagt tggaagttaa aacaaatgtt
     1381 aattctctgg aaatagagtt ctattcaatt ttacaaaaaa aactggaaga ttacacggaa
     1441 aaattgcttg cagtcagtca tttacctctg gtattaacaa atactgattt aaagcgacta
     1501 tttcagataa gtgatagtac actgaaccgt ttagtaaaac ttggtgagtt tcctccctgt
     1561 tggtacggga ttcgaggaca ctattttcgt gatgatgttt tagaatggat gagaaagagt
     1621 gattcgaatg actatcgaga aaaattgcga ctacttcgat ctttataagt tacattttct
     1681 ttctaagatg cggtacaatg atgacataga tagaaagatt tccaaagggt ttctgcttgt
     1741 tgggttcctt caaaatagga ggaatcaata aatggaaaaa acagatcgat tcaagaagca
     1801 aatcagttct actggtagga tcacatggta ctttcaagca tatcgaacaa caagacgtgg
     1861 ctttaattca aaacgtgagg cgcaactcgc ttatctggaa atggagaaac aacaaaggct
     1921 taagaaaaaa attgttgcaa gcaatgacaa gtttagtgtt gtaggtgagc aatggttcca
     1981 atattaccgt tcgttaaatg aacaaaagga aataacctat gataagcgag aggaacaatt
     2041 aaaaattatt gttcgatgga ttggtgataa aaagttgtct gatttgacac cagagtgcct
     2101 tcaagaattg ctttttacct taaaggaatg tggcgttaat ggaacaaatg ttggctatgc
     2161 caaaaatagt ttacaatcac ttattcaagt tctaaatatg atttttaaat attgtcaaag
     2221 aaagaatttg ataagtgaaa atccaatgaa gtctgtgaga atgccaaaat atcagttaac
     2281 ggtaactgat ttaaaagaag ccataaataa tattgaggac aagtttctta ctgtcgatga
     2341 attgagaact tttttgaatt atggcattgt tcatgaggaa ttaccgatgt ctgtactttt
     2401 ccatgttcta ttttacactg gctgccgagt cagcgaggcg cttgctctac agcctggaga
     2461 tatagatttc aaaagaaatg aaattctgtt ctataagcaa acggcagtta aaggtaagag
     2521 taaggatttt cgaattgaaa ctactaagac agttagttct gctagacgag ttctagtaac
     2581 tcctctagtt atggaaaagc tacaacagtt gattaaggct ttgggtgaga tgaggcaaca
     2641 ttacaggttt attatagaag aaacatattt gtttgtgtat cttgattcta gtaaacgtgg
     2701 tgttccttat cgtcgagagt atgtaaatga tcacgtcaaa cggtgcgtga atcgctcagg
     2761 aattaataag ccatttcata ctcatttagc tcgtcataca atggcaagct tagtagcgga
     2821 acactgtagc tgggaagtct tgaaagatcg cttaggccat actgaccaaa caacttcgaa
     2881 aatttatcgc cacttaacgt caaatgaaaa ggtaaaacct ctgattgctt ttagttcttt
     2941 agagagttaa aaaaacggtg aaagcactgg tattacagga ttcttgaaaa acgatttttt
     3001 aacaaattag tcaatatgta gtcagaggta ataaaaatcc ttttatatca atactttaac
     3061 gacttttcac aagtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa
     3121 cccttggtat tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt
     3181 tttctttaat tattggtagg ggagaataat ggattagaat aaatctacct ataagtagta
     3241 gtaaaaaaaa gagcactatg ataatatctt tcttaagagt aaccgcttta ataaaaaatc
     3301 cagaatattc taacataaat ggagtattct tagaatcaaa aggagaaaga agatggttta
     3361 tacttatacg gagcaagaaa agatgaaagt tttagacaca ttcatgaatg atggaaaagt
     3421 aaaacaagta ccaagttcaa caaaaaagaa atacatccta ttgaaagaag tcttgaaacg
     3481 ctttgaccat ggagtgactt atacagaaac agaggttaat tcgatactgc tgaacgtatt
     3541 ttctagtggc gattatgtcg aacaaagacg ttatctaatc acatttggct ttttcaaaag
     3601 aagttcagac ggtcgggcat atcagatgat gggaattgaa aattagaagt tttaatttat
     3661 actcattgaa aaaagttcaa gtatgcgata ttggttctac accgcatact tgaacttttt
     3721 ttgtcatctg tatctataat acaaagccaa gaacgttatt caaaatatgg atagctatga
     3781 taaaagctaa tgagcgattc gcagttaaat aagcaatcgt cagtaaaatc gttccaggag
     3841 cgtacatgaa aaaatcaatt ggatgcgtta catgaataaa gataattctc ttttatttgt
     3901 acgaataaat accaaaacgt acctcctaaa attagatttt ggacagatgc cctttaaaaa
     3961 gataacaaga tttcgttctt gaaacaatat atatgctttt attccaatga ggttgtgaaa
     4021 acagtatttt gtcctgagcg ttagaaaatt gaaaatgggg aatagcaact cgtactttac
     4081 cattttttaa gtgaatacaa gatcagctag ttgaacaccg tttattcgtg atgagggaca
     4141 caacactgca gtcgacacat ttctctttta tgttcaaaga taacaaatat ttaaaacaaa
     4201 gaaaaatagt agataatccc g
//
LOCUS       DAFGZE010000138         4177 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.142, whole genome shotgun sequence.
ACCESSION   DAFGZE010000138 DAFGZE010000000
VERSION     DAFGZE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.142"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="LTU54_002931"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="LTU54_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HBL8297564.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAITVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="LTU54_002932"
     CDS             complement(2945..3379)
                     /locus_tag="LTU54_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297565.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..>4177)
                     /locus_tag="LTU54_002933"
     CDS             complement(3396..>4177)
                     /locus_tag="LTU54_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL8297566.1"
                     /translation="EDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYDPEIYLFLYENK
                     ILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDI
                     IEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIG
                     LNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTIS
                     EVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT         1269 a          786 c          582 g         1540 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttat tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttcta
//
LOCUS       DAFGZE010000139         4100 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.143, whole genome shotgun sequence.
ACCESSION   DAFGZE010000139 DAFGZE010000000
VERSION     DAFGZE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.143"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            52..1353
                     /locus_tag="LTU54_002934"
     CDS             52..1353
                     /locus_tag="LTU54_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297567.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1380..1640
                     /locus_tag="LTU54_002935"
     CDS             1380..1640
                     /locus_tag="LTU54_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBL8297568.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2639..3874)
                     /locus_tag="LTU54_002936"
     CDS             complement(2639..3874)
                     /locus_tag="LTU54_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL8297569.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1167 a          664 c          860 g         1409 t
ORIGIN      
        1 gcgttgtcat gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag
       61 ctgatgaaat gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt
      121 catttattag caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca
      181 ttttttatgg tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat
      241 tcggcaacga ttgttttacc ctttaggatg acgatgggat tgatggctat ttatgcaact
      301 tttgttatgg gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata
      361 ttatctttag gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaagag
      421 gcacctcttg gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg
      481 atcttatcta tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact
      541 atcaaaatgc cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca
      601 ggtgcagtca ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac
      661 gctgccttat tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt
      721 gttttactac caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt
      781 attgtagggg ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca
      841 ttggcagatg gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa
      901 tggacggtaa caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc
      961 ttctgtaaat caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc
     1021 aatatcaatg agcccataat ttttggagct cctatgatgt tgaatcctta catgttcatc
     1081 ccgttcaact tagttccttt agttttgact attatcagtt attttgcagt caaattagaa
     1141 atggtcaata gatttactgt tttgccaaca tggacattac cagctccaat cggcggttat
     1201 ctttcagcag gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca
     1261 tttattatct attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa
     1321 agtcgtggag aaaacgaagt ccagactgtc taagtttggt ttttagaagg tgggaaacaa
     1381 tgtattggct gattttattt tttgttttta tctttttatt aacggctagt caccttatat
     1441 tgaatatgct agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat
     1501 ttttgatcgt gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg
     1561 ggacatatgt actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt
     1621 ttgtcacatc gaagctttaa atgttctaaa agtggttgta acagaagtgg ttagcttcaa
     1681 gaaataagaa gaaatttacg aaaattgctt cttaaatttt tgtgaaatgt tggcttattt
     1741 ttcgtttggt tgaacttatt accgcagatg caagcttttg accaccgttt attcggaata
     1801 tgggactgtg acaaggcttt tgtcacagcc cttttttaca taaagattat tattgtttta
     1861 actgttttgg cagaaagaag gaggaaaaaa tggaaatttt atcagcaatg gatttagaat
     1921 tagtagatct actaccaaag tgatcaagtc aaatatagat ttgggagctt acaaccatat
     1981 agtaggggct gcagtatggt gtatgaatgg taatatgtat gatgtgagtg tttattcaaa
     2041 tcatgaaacg tgtatagaaa taattgtgct gggaatagca atcagtcatg gtgaacgtga
     2101 cttttagtgt atcgttgctg acgacataga acattctgac atgatctact caatatgtgg
     2161 ggattgctgg cagtttatat aggagtatgc atcggattgc gagatcattg tctcaacaga
     2221 agagggaggt tgattatcaa agataaagtg gtaaatccta catttgattt agtaattgtt
     2281 gaggggacta gcggcacaaa tctctacaaa gcgatgcttc tagatctata tgacacattt
     2341 taagaaaatg tatttgccta attattatgg tctttctttt gaagaaacgt tgagatgatc
     2401 caaccagaga aaggtgtcaa agaatttgac taagaataaa tggcagacgc tacctatgct
     2461 catttggata gcgtcagatc ttttatttta aggatgattt tgtgtttttt agacaccgta
     2521 atttaagtaa ctctaacttt tcaatccact atttactttt tttgtatttt cactgtctgg
     2581 gtctcgaata gcgaaattac tgatgagtct ctattaaaaa agctttttct acttgaaact
     2641 atattgggtg gttgctcagg aaggtttcga tttcttgaat tgcttgttgt ggaccattta
     2701 ttggaaaaac ggcttgcagc gtgcgtgcct gctgacgaat cttgggtgaa gaaacttcgc
     2761 gcagagcaat ctgcagtttt ctttcagtca ctggttttgt gagggcgatt gcgtttccgt
     2821 attttacaca atagtcaata ttacagcttt gctcaaattg catccccaca ccgataaacg
     2881 gtttgccgga ttcacaggca gtttggacgg tcccttcccc gccgtggata aaagaaaaat
     2941 caatgaattc atgcaaaaga tgactagaca gatattctgt tattaaaaga tcttctgagt
     3001 cagattgaat gtcatcaagc agatagtcaa taggtgcaat aaccgctaga tccggttgtt
     3061 tttgtaagaa gaacaaaatt ttgcgtaaca tacgcggatt gcctgaacta cccattgcga
     3121 aatagataat acgttttctt tcttgttgct ttcgctgtat aaaagttagc acttcttcag
     3181 gcaaggaagt actgagctta gcaaaaattg gaccaacgat tcgataattt tccggcagct
     3241 gttcttcttc caaaaatagt ttaggcgttg tgatgagatt tagatcagca tccatcattt
     3301 gtaaacttgt ttttggtaaa ggaagcattt ctttttgtgc agcgtgtttg aaggcattgg
     3361 ggatccatcg ggtatttact atacgctttt ttgtccagtg ccaaagggga ttgcttaaag
     3421 ggccgaagaa acgtaactcg tggggcatca gcgcatgctc tgattgaaaa aaaggttcac
     3481 tcaaagcata gggtttaaca tatactaaag gaatcttttt attgcgggcc gataaaaaaa
     3541 ttgtcacatt cgaacctgtc acaatcagtc gaggctcaaa cttagcaatc agactctgtt
     3601 cactagtgat acgagaggca accatttctt tggtaaaagg gtttttaagt gtacgcaact
     3661 ggtcaaattt tatgatttgt tcttcttgtt ttttcgtaag gatcggcgct agtaaatgat
     3721 actcaaagcc actttctgtg atcaaaggag caaaagtttt agaaaagcca aagaatacac
     3781 agtaataacg gtctttcata gctttggcaa cctcaatcat tcgtgttgtc tcagctaaat
     3841 tgaatactgc aggagcaaaa agaattgtct tcatgaaatc ctccttattg gaatgagtct
     3901 atcccttcgt ttcttactag tatactaaaa aacacagcgt tcgagtagcc tatgtatatt
     3961 ttgaatatta aactgtctct cgctgttttc acaacttcta aatttaaatt ttgtaatcaa
     4021 gttgaaactc caattttcaa aaaaacacgc aacaattttt tgatcgattt atatgttgtg
     4081 ccagataaat acgagaagtt
//
LOCUS       DAFGZE010000140         4066 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.144, whole genome shotgun sequence.
ACCESSION   DAFGZE010000140 DAFGZE010000000
VERSION     DAFGZE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.144"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            57..509
                     /locus_tag="LTU54_002937"
     CDS             57..509
                     /locus_tag="LTU54_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297570.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            523..1212
                     /locus_tag="LTU54_002938"
     CDS             523..1212
                     /locus_tag="LTU54_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297571.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1337..3688
                     /locus_tag="LTU54_002939"
     CDS             1337..3688
                     /locus_tag="LTU54_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HBL8297572.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3768..>4066
                     /locus_tag="LTU54_002940"
     CDS             3768..>4066
                     /locus_tag="LTU54_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297573.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFK"
BASE COUNT         1406 a          521 c          833 g         1306 t
ORIGIN      
        1 aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg agaatcgtga
       61 aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc tattttcttt
      121 tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta gctgaacata
      181 ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa gaattgtata
      241 aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga attagtgact
      301 cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc aaatccgaat
      361 tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat tccaacgcta
      421 tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta aagcagataa
      481 aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa gatttttggt
      541 gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc tgctgagaaa
      601 gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt tgaagaagaa
      661 gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa aattgtatct
      721 atggataatc cagatataat tcaaggttca tataattcag aaactgatcc tgcttcagtt
      781 tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat tgaaactact
      841 ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca ccttcttgta
      901 ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg gcgttttgga
      961 ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa atcctacatt
     1021 gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg ttctattcaa
     1081 ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg gcctggttgg
     1141 acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta ttatgttgtt
     1201 caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt atttttagtt
     1261 gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat gttcggcaag
     1321 aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc taaacaagaa
     1381 atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag ggtggcctac
     1441 aatctatgga cttattgaag gactacaagt agaacttggt attactaatc tagtagctaa
     1501 ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat ggggcaaaaa
     1561 tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg ggattaatcc
     1621 aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag agttgcagac
     1681 tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat gggccaaagc
     1741 gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa ttagaaaaat
     1801 gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt gtgatggtat
     1861 ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac taggatattc
     1921 tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt atcctgtgtc
     1981 tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg ttaatggtta
     2041 ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag aagtactggc
     2101 attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg ttgtaataaa
     2161 aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag atacttctac
     2221 gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa tagttggtag
     2281 atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc taactacaga
     2341 ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc aagatggagg
     2401 tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga aggctataga
     2461 tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg ttgatctaga
     2521 catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg tatctgctgt
     2581 aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt gttctcaaat
     2641 aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg gttatagtgg
     2701 gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg aatttaccat
     2761 aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta gagatagcgg
     2821 atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat acaatcagaa
     2881 ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc ttgataatcc
     2941 ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt catgggacat
     3001 catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata ggaataaatt
     3061 aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt caataatagg
     3121 tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca caatcaatga
     3181 tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg gtgaagttaa
     3241 gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg ctgcaggtgg
     3301 aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt ataccttagc
     3361 aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt gtgataaggg
     3421 tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag ataaaatcgc
     3481 ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag gtgtagggat
     3541 aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt ttacttatct
     3601 tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa ttattcaatt
     3661 tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag tgtccttttc
     3721 ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg atgattatgg
     3781 aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga tgtggaatta
     3841 tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc aagatagtag
     3901 attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag tttgaaagag
     3961 taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa gagtctccaa
     4021 atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaaga
//
LOCUS       DAFGZE010000141         4031 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.145, whole genome shotgun sequence.
ACCESSION   DAFGZE010000141 DAFGZE010000000
VERSION     DAFGZE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.145"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(243..392)
                     /locus_tag="LTU54_002941"
     CDS             complement(243..392)
                     /locus_tag="LTU54_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297574.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            854..1066
                     /locus_tag="LTU54_002942"
     CDS             854..1066
                     /locus_tag="LTU54_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297575.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1344..3143)
                     /locus_tag="LTU54_002943"
     CDS             complement(1344..3143)
                     /locus_tag="LTU54_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBL8297576.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3267..3863)
                     /locus_tag="LTU54_002944"
     CDS             complement(3267..3863)
                     /locus_tag="LTU54_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBL8297577.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          712 c          689 g         1181 t
ORIGIN      
        1 tatattaaat attatacaga taataacgga atattatcat cgaaacagct aacaaaattg
       61 tcataaaaaa cgagatggtt gatattagaa cttgtgtttt tgtttaggtc tagagataaa
      121 gagagacaat ttccaaatat atgtaatgaa tacaaaaaga cgtttgtcgc ggattaccgt
      181 tacaaacgtc ttttattgat tttccgagat ctcatcagtc gccatttgga atggttcact
      241 ccttatctag attcgtttaa tgctcggact gcttgttcga ttgtaatacc aaacgcaacg
      301 ttattttcgt tttgtgcatc aatcgccata aaacggttag aagttgtatc aaaagctacg
      361 cggtattgca tatttgtttt tgtaaacgtc ataaaattca tctcctttgt aatgtaattt
      421 agttcttgaa agtttccttt aaactttcga acttatacca agtttagcat cgtacgtttt
      481 ttgatgcaat taaaaagcat ttatcatttt ccttatttat tttttcttaa atggtttagc
      541 tattttcttt taatccgata agtagcccaa tcatcaaggt tacaaatact attgcttgaa
      601 tataaatcat atgctcacct ctttataaat aacttagcaa tataaagaaa aaaacgcacg
      661 aacaacatag gaatataaac aaacgttttt gtcacaacgt ctaagatcgt gttaaaaaat
      721 agttatctaa aaagcagaaa gatactcaaa ataaaaaata aaaaaatgga taaaaacgtt
      781 ttcaaataca aggtaatttg ttattataaa ataaagaaaa aacaaaataa aaataaagaa
      841 aagagggaat gaaatggtaa aagcagaagt aggaagatct tatatatgca aaccgatcgg
      901 agtatctcat gaactaatcg gctgtattga acgtacatat atcaacacgg ctgtcgtgta
      961 tgttgaaagt tatgaccaac gagactatca acttatcaat gaaagcaagt atcgcatgct
     1021 cgtaaaattc tgtgatatta gcgcacctgc tgaattggtt tcttaaacag tatttttgag
     1081 aattcgcaag aactccctta taatataaat aaagacagaa gggagcgaga agatatgcca
     1141 aaaagaaaaa gttcagggaa aatgggaatt attcttgcag tgatctctgc aattcctgct
     1201 attttagcta ttgttcgtgc gatgaaggaa tcaagagaaa gttaagcaca cattatgagc
     1261 gtcaaacaaa aaacgcatat acctgcggtg gcaggttatg cgttttttgt tgtataaaga
     1321 cttttttcat taacgatttt tgattagccg actcccaaaa caaaatttag cagtttatct
     1381 gaataagtga tttcttcatc tgtttgtttc acactttcat ttagtagtcc catctcatcg
     1441 gttatgatac catccacacc ataaaacatc atgcgactca tagcatctga atcgttgatt
     1501 gtccatacat atacttcttt tccgtcttgg tgggcagaat taatgaagtt acggttgatc
     1561 gtactatatt ccattgtcag aaaatcagca ggcgtcacag ggggcccaac aatattgaaa
     1621 ggtaagatgt agcccacata aaagtttggt tctgcttctt ttaaatctga aacggtttgg
     1681 tacgtcaaac tttgcaaaat atgatggttc tctaagataa ctttgcggta tttatggaca
     1741 aatcgttcga caaggtcatc cgaatctttt ttagaggtct tgatctcaat aagtaatttt
     1801 tggtttagct tatgtgcttt ttccaaataa gaatcaaaag aacaaacagg ggcttgcact
     1861 ccgttttcag taactgtaag atttgtcaat tctttcaatg taagatcctg tggtgttcca
     1921 tttacccctg ttaagtgacg aagatttgca tcatgcatca ctacgaaatg accatctttt
     1981 gtttcctgaa catccatctc tacataatca ggatgatagt ctctgcttgt tttttcaagt
     2041 gcttctaaag tattttgcgc cccattttgg gtactcacac ctctgtgaga gacagtcact
     2101 ggttttgtct gaacagctga agtcagataa tccatattgt aaagacagac gccaataccg
     2161 aaaaaaactg caaaaagagt caaaccagtg tttttcagcg cagagaaacg caagttgggg
     2221 gcttgtggga taaaccactt agggatctca ggcaagaagc cttcgtcatc catgaaatca
     2281 acaatgatat aaaaaatccc tacggtagac atcacgatat tgaataataa aatcccttgt
     2341 aaaagcgtca tagcaaacac agcgctgatc aatgcatagt ctgggaaaaa ttgttcaacc
     2401 atactttgtg ccaaaataac aacgatatat cctaaagaag aaaggagtaa aatcgtaccg
     2461 ccaatcacaa taaattggcc cgtgatggcc aaaagacgcg atttggtcaa tgaccagctt
     2521 tcccgaatgg ctgctcgaaa aggacgatcc cgtagaatca tttccggtaa agcaaaaatc
     2581 agtcgaatcc caatatagcc aaggaaaata taaacgagta aaaaggaact gacgatgatc
     2641 caacgattag caaaaataaa atccataatg aaagcgggga tttttatttt tgacagtaga
     2701 tcagaattaa aacttaatcc gctgatcggt agaatcaata aaaaataagc aaggaaaaac
     2761 aaaaaagtaa tgggtctcac tttcttcagc tgaagtatcg tcaagtgtaa gagttgcttc
     2821 aaagagattg gttctttctt tttaataaaa aaaacactca tcaaaagaaa agtaaattcg
     2881 aaataaacta acagtaaaat cgtcatcaaa actacgatta acgagagtag aaccccagga
     2941 tgttgagaaa aaattgaagg aatattgtca taagacaaat agtcaattgc ccctcggtgt
     3001 aaaataaaac gggtcgtact gctcaataag ggtaaacaaa taaataaaat aaagccatgc
     3061 atcagcaaga catcacggaa ataagcttgt gttcctttta aaaagtccca agcattacct
     3121 aagctacgtt tcaaataatt catcctatca cctgctcttc acattgtagc gcaaatctaa
     3181 tgaaaaagga aagggtgcag tgtgttttgc agaccctttc ccagatttaa cattgtcaat
     3241 caatgactaa ttttacaaaa taaatattat ccaagaagac cacttagata ttgacctcca
     3301 taaatcaagg ccatactata taagaaaatc gacagtggtt tagcaatcaa aatgattagt
     3361 gtatatcgtt tcactgacat gtttgtcaat ccagccatca aacataacgc atcgtcagga
     3421 gcaactggaa gaaaaatagc tagagcaaac agcttctcaa aacggttttg attatccagc
     3481 cagccaatat atttgttata tgtcttatcg ctaatcatgg aaagtatgaa tggctttcca
     3541 taacgacgcc ctagcagaaa aatgataatc gaaccaatgc agattccaat atagttatag
     3601 ataaatccag cataagggcc aaatatcagg acaccagctg cagtactgat ccctccagga
     3661 atgattggaa taactacttg caggatttgg atgaagacaa aaataatcgg tcctaaaatg
     3721 atggaatcac ctactaatcc tcttaacgca ttcaaatctt tgaaaacacc taaattaata
     3781 aaataaatcg tcagcgcgat tgataggcct aatccgataa tggatatgaa atttatcagt
     3841 tttcgagagg cagcaatatt cattattcat caactcctta gaggttcttt gttaactata
     3901 ctttactaga aaagcaacta actgcgccat tttttgatat gacagaaaac aaacaatttt
     3961 gtaagttcat cataagtata aaagattgat ctcaaaaaaa catccaacta aagacttatt
     4021 taataacttg t
//
LOCUS       DAFGZE010000142         4011 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.146, whole genome shotgun sequence.
ACCESSION   DAFGZE010000142 DAFGZE010000000
VERSION     DAFGZE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.146"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(69..611)
                     /locus_tag="LTU54_002945"
     CDS             complement(69..611)
                     /locus_tag="LTU54_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HBL8297578.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            746..1135
                     /locus_tag="LTU54_002946"
     CDS             746..1135
                     /locus_tag="LTU54_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297579.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1139..1903
                     /locus_tag="LTU54_002947"
     CDS             1139..1903
                     /locus_tag="LTU54_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HBL8297580.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2032..2979
                     /locus_tag="LTU54_002948"
     CDS             2032..2979
                     /locus_tag="LTU54_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBL8297581.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3109..3849
                     /locus_tag="LTU54_002949"
     CDS             3109..3849
                     /locus_tag="LTU54_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297582.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1337 a          681 c          810 g         1183 t
ORIGIN      
        1 ataataaata aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag
       61 tttgtgagtt attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata
      121 tattttggct cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac
      181 agttcacgtt cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc
      241 cccattgcag aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt
      301 ttatcttctg cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat
      361 ttgtcttcaa tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat
      421 aaattcagtg gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg
      481 atctcgacca tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa
      541 cgtaacgtgt tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt
      601 ggatcaatca tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta
      661 agtgaagaaa aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat
      721 tataaagata ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca
      781 cgtgctaagt ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga
      841 ggtagagaac attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt
      901 ctttttgata ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc
      961 tatttatcca ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt
     1021 tcccgagcca ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac
     1081 tgttggagca tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt
     1141 gaatccaatt atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa
     1201 agaattaaga gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa
     1261 ggagttagtt ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc
     1321 agatgcgatg aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt
     1381 ttgggatagt atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa
     1441 aagaggtctt ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac
     1501 atgcgccttg ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc
     1561 tgcacctttg gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt
     1621 accaaaagac tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc
     1681 ggaaaagtta gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc
     1741 ttatatacaa acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat
     1801 tgtttttcgt tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc
     1861 ttccactttt ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac
     1921 tcatgacgat agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa
     1981 atttggtata attgatagta agaatgacaa catgttagaa aggaagagag catgcccact
     2041 ttagcagatg ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat
     2101 catccggaac aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg
     2161 gattaccgtc caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa
     2221 ttatttatac tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga
     2281 atcgctaaac ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc
     2341 gacgtcggtt cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg
     2401 atcgaccgtt tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat
     2461 gtagacagcg ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat
     2521 tatcaaacga ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa
     2581 actggttatt tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca
     2641 aatcattcga cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa
     2701 acttgttttg tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat
     2761 gctggaggac acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac
     2821 caaatcagtt cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta
     2881 tgtggagaaa tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt
     2941 ttttttcctg aactagttat cagagagaca acgaattaag tcttattact atatatatat
     3001 ttatgaaatt catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa
     3061 tctgtgctat tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc
     3121 agacgaaaac atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac
     3181 gacaagaagc gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc
     3241 ccgtttgaac agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc
     3301 aagcgtcgtg caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat
     3361 aaaggaaaat taccggatta taccggccat taccaatcag aaatcacatc gacaggacga
     3421 aaaaaacttt ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc
     3481 aagcatacta cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt
     3541 cctaaatatg tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat
     3601 catttaatcg aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg
     3661 acgccgtatc aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa
     3721 acgttagagg aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta
     3781 gaacgctcat tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat
     3841 tttaaataag tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg
     3901 tttccttatt ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt
     3961 aagcgaattt tttctcccaa taagtaagaa aataatggca aaataaaaaa a
//
LOCUS       DAFGZE010000143         3866 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.147, whole genome shotgun sequence.
ACCESSION   DAFGZE010000143 DAFGZE010000000
VERSION     DAFGZE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.147"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(248..469)
                     /locus_tag="LTU54_002950"
     CDS             complement(248..469)
                     /locus_tag="LTU54_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297583.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(567..812)
                     /locus_tag="LTU54_002951"
     CDS             complement(567..812)
                     /locus_tag="LTU54_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297584.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1052..1666
                     /locus_tag="LTU54_002952"
                     /pseudo
     CDS             1052..1666
                     /locus_tag="LTU54_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2036..3037)
                     /locus_tag="LTU54_002953"
     CDS             complement(2036..3037)
                     /locus_tag="LTU54_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HBL8297585.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3196..3462)
                     /locus_tag="LTU54_002954"
     CDS             complement(3196..3462)
                     /locus_tag="LTU54_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297586.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3452..3808)
                     /locus_tag="LTU54_002955"
     CDS             complement(3452..3808)
                     /locus_tag="LTU54_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL8297587.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1222 a          698 c          512 g         1434 t
ORIGIN      
        1 tttttactat atacttcttc ccagaacttg tccgtctcct tcaaattatc tctacctctc
       61 gagttcattg ggtggtttgc ccatttatta aacatggaat cacctttctc atactcatat
      121 atttatgtcc tatttcaatt atatttatat atgcttaaat aaatttgtat cctgaaattt
      181 attgtaatat gaagctgtat tttacatata gtaaccaaag agttaataaa ataaaaagag
      241 cgaaccatta tagatcagct cttttttcat tgctgttttt aatcttatct agaatcactt
      301 caaatgtttc aaaggtattt acatctaact gacataacca aagcaagtac ttcttttctt
      361 tgtaagtcca atcaatatta cggtaagagg acattagatt atggtacctt gctctttgtt
      421 caactgaagc caattcccag cgtttttcaa atattttttc ttcttccatt attgatccct
      481 cgcaagacgc tacctcaaac ttaaataatg ggcagcgaaa gtctaattcc ttagatctaa
      541 gtatagcaaa caaatatgaa aaaaacttag aaggtagtaa aagttcttcc gtcttttgct
      601 ttgcttctat tcttaatgtg tttcgttatc ggtgccctac aaagattttc attaattaca
      661 cactcattta ttgttgtgta ttttctgaca tttctctctt gatcaattat tttaattcca
      721 tgggcatcct gatagttcat aggagaggag cggctatcag aaatagttgc acctatctct
      781 tgaacttctt ttacaacttc tggaaaatcc atccatcctg cttcagctgt caaaagtaat
      841 aatctataat atttatcttt ccttttcaat ctaagaaatc gtcaccccat tctttttctt
      901 ctattataaa ctatttaact tttagaattc aaaaaccgtt tgtctattta gagtttaccc
      961 taaatagaca aaaatataac attgaaaaat ttagagttct aacgtatgat tagggtgtat
     1021 tataaacgtt atagaaaagt agggaataaa tttgaaaatt gcttatatta gagtttcatc
     1081 catcgatcaa aatgaacaac gtcaaattga agaaatgaaa aagtttggcg ctgaaaaaat
     1141 ttttatcgaa aaacaatctg gagcgacgat tagtcatcgg ccgattttcc aggaagcttt
     1201 agattttgtc agagagcaag atatttttat tgtagaagcc attgatcgac ttggacgcaa
     1261 ttatgacgag attatcgatt cagtgaatta tctaaagaag aagaatgtgc gacttattat
     1321 caccagttta ccgatcatgg ctgaagcaat tggaaatcct ctcctagata aattcatcaa
     1381 agacttaatc atttaaatct cagccatgat tgctgaacaa gaacgtactg aatcgaaacg
     1441 acgccaggca caaggaatta aaatcgctaa agcaaacggc gtctataaag gtcgtcctaa
     1501 gttatacagt gcagacacca aagatcctca acgtcgcctt gtctatagga gtatcgttca
     1561 ggatcttgag aatggggttg ctatctcaaa gattgcaaca gactacaatg tgactaggca
     1621 aacaatttac cgaataaaaa aagaaatcga ccaactaatt gtatgatttc ttttttgaat
     1681 cacgtaatta ttaattagct ttgtcatcaa tacagacaat cattttagat tcatctagct
     1741 aagttgatta catttatgtt aaaaagaatt tagttaacat aaatataatt gatgcataaa
     1801 tcgtccttat ttaaaggcat ttaacattga caacttagta taatgtatct tatgtaaact
     1861 aataagattt gaattttatt ttatacacac tcgataatag atcaatttta tggtcccccg
     1921 catttacgat tgcacaagag aaacataaag tcgacctatt agcatttctt ttcaattcaa
     1981 acattaaaaa agtttatacc tctgctacta aaaaatgaaa ttatctattt aaaatttaaa
     2041 acggtaaatc tgattcatcc aaattggaaa atggatcagc ctgtattgga gctttgtatt
     2101 tctccataat aacagatatc tttttttcat tttttaaaaa atacctttta aatttcttat
     2161 agtcctctga aatcaaaata tagcgtgatg gatactcatc aaaagaagaa tcgtattctt
     2221 cctttaaaat tcccgattta cttaattttt ctagtacatc aaatactcta tcactcaagt
     2281 aactccttaa attgttatca ctttcccatt gaactacttt tctataagtt gaattatcag
     2341 ttgaaaagat agaattttca tccataaacg catatattag cagcaatctt tcatcattgt
     2401 caaattctat tggatcaaaa atatctaagt tgtcatcaaa atctttatca gttccggtaa
     2461 cttttttgtt aattactgaa ggtgtcttgt aagaaataga atctaaatca aagcgttttg
     2521 attctaaatt attttgtgct ttctcaatta aataattcac tttttttgtg agaaaccttc
     2581 tgatattgtc tttgctatat gttccataaa aacccttatc ttctaaagag tccctgtact
     2641 tcttaacttc tatatactct tcagccaaaa cttgtggaac tggttcatct aagaaaaaaa
     2701 cccctacctc gcctttgcta tttttataaa agtaatcaat ttctgctata gttcccgatc
     2761 tataattatc aactggagaa cccaacttat ggccaaaaat cgctaataat atatcactat
     2821 tttctacaat ttgtttatta ataacacttt gcccagagct attatttata taagtaggaa
     2881 tcgcattcgt ttcccatcga attggcatca aaattacttt tgtattatct gtattttcta
     2941 tattccatct atatatggtt tcttctactt cattcctctt tgctgctata tcacctggtg
     3001 atgctataaa tacttttatt agatttatgg ttttcattta ttgtaccttt ctctttatgt
     3061 ctctttaaag taatttttgt ataaaattta ttgactttta cactatagga caacttttat
     3121 ctatttagta caaaaaggcc cactaaacta attgattagt taggagccta ttacaggtat
     3181 attttcaact cgcttttaag caaattctac taccagcttt ttgcctaatg ccctagcgat
     3241 atcatcaagt ttatcgaaag atacgttatc ccctctctca attcgagcaa tagtagattg
     3301 agttgtatgt gctttttgtg caagctccgc ttgagttagg cctgcttcct cccgtgcatg
     3361 ataaagagca accgcagttt cgagttttct tccttcacgg tcatatgctt ctgcaaaatc
     3421 tttgtctttt tgcttttctt caatcaattt tttaatcatc atttgtgaac cactcctctc
     3481 taagttcttt tgcatgttta agttcactta ttggtgtttt ctccgttttt ttagtaaatc
     3541 catgggtaat gacatacctt ccattgacca catggaaata gatagctctt tggatgtttg
     3601 atcctacctt acttcttaat tcgtacaaat tagtgtctaa ttttttcacc catttcaacc
     3661 gttgagctac tagcatccca ttcttttctg tttcttctat tgtacgtaaa agcttcgcac
     3721 tatctttttt tggtaatgtt tccaaccatt ctaaaaattc atcatgacca ttaggtcttt
     3781 taaatgtttc aaaagatatt ttttccatac ccaattatat catatattga ttttatatta
     3841 tagtatataa cataaaaatt cgaatt
//
LOCUS       DAFGZE010000144         3797 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.148, whole genome shotgun sequence.
ACCESSION   DAFGZE010000144 DAFGZE010000000
VERSION     DAFGZE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.148"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            4..1131
                     /locus_tag="LTU54_002956"
     CDS             4..1131
                     /locus_tag="LTU54_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8297588.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1349..2491)
                     /locus_tag="LTU54_002957"
     CDS             complement(1349..2491)
                     /locus_tag="LTU54_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8297589.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2496..2681)
                     /locus_tag="LTU54_002958"
     CDS             complement(2496..2681)
                     /locus_tag="LTU54_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HBL8297590.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(3035..3280)
                     /locus_tag="LTU54_002959"
     CDS             complement(3035..3280)
                     /locus_tag="LTU54_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297591.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3274..3675)
                     /locus_tag="LTU54_002960"
     CDS             complement(3274..3675)
                     /locus_tag="LTU54_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8297592.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1250 a          686 c          574 g         1287 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat caatgatgat
       61 ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc cgaagctgct
      121 ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg tacggatact
      181 cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa tctaaaatac
      241 aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta tttaggaaag
      301 aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc tgaatttaaa
      361 aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa tgcgctgaat
      421 atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt aaaaatacca
      481 aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct tgaggacttt
      541 gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt gaatattgga
      601 ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga tgtcgatttt
      661 tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc atgggtatat
      721 ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac cttgctagca
      781 attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg aaagctctac
      841 attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc aagtgtagtg
      901 aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt ccattctctc
      961 agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga aatctctgaa
     1021 cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt aacagataag
     1081 atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg atttttgcca
     1141 ccgaaaaaaa ccaccggtgg caaaccggtg gcaaatccat gtaaacatgg tttatttttt
     1201 acactgtttt caaacaaacc cttgctattt cttcggttcg tcctcgtcca ttttcgatac
     1261 cgacagctag cttataaccc aaggtatatc aacataaaaa aataaaaata ggctatgaca
     1321 gcagcgtgac agcaaaaaag atggaaagtt aaatatcaaa cctcgtaatt tcatttgcga
     1381 gtgcttgatt tgtttctgga tataagtgtc catatttgtc caaagtagtt ttaatagagg
     1441 catgacctag acgcttagat attgcataga tatttttatt atgttcaatt aaaaaggcaa
     1501 catgcgagtg tcttaatcca tgcatatgaa ttggttttac tcctgcaatt tcagctagtt
     1561 tttttattct gcttaaaata gttcttggag atggcggtaa tccatcaaaa gaaaaaacca
     1621 attctatatt tttcccaatt gattgttgag tttttttcca tgtttttaaa tcttcaacgg
     1681 tttgtctgtc taacgaaatt atccgaatac ttgatgtatt ttttgtttca gcaaattcat
     1741 aatcttgtct tgtacggatg taaattgact tatttacgct acatctgcca tttattaagt
     1801 caaagtcttt ccattgtaac gctaagagct cctcactcct tacacctgta acaaaaataa
     1861 aacgcatcaa acgtttataa aattcttcat aaaaatcccc gtgataaaca ctattatata
     1921 ctttcttaaa ttcatcaact gtccaaaatt ctacttgagg acgctgagtt tttattttac
     1981 ctatagtatc tacaggattt tcttcaataa tttcaagtac aattgccctt ttaaaaacta
     2041 ctctcaactt attgaatatc tgtttaatat aatttggtga taatggttgc tcaatgccct
     2101 tttttacaac atggattttc gataaaaact gttgaaattc ctgtatatga attggtcgaa
     2161 tctttacaac ctgcatttct ccaaaaaaat ccaatgcacg ttgtaaaata tgatctgttt
     2221 tcatatatgt tttttctaca gttcccaact tataccaagg aaaaaaatac tctcgataaa
     2281 aatcatgaaa cgttattcct tgcagataaa ttccttgtga atcagaaaaa ctttgtttta
     2341 actcttcgag tccccttcta gcttctcctt gagttttgaa gcctcttctg gtcacttgaa
     2401 ttctttttcc aaagcgatct tgtcctaaag ttgcagaaaa aaaccaagtg cctctttttc
     2461 tgtctttata aacattcgca attttcctca ttttactatt cctcccaaga aatatggaga
     2521 acttcttcta aaattgaaat aggaattctt tttatttttg aattacgata aaaaaagtat
     2581 cctttctcaa ctaaaagttc tttacattcc ttgaagattt tattagcata gtactgagaa
     2641 aaacctagat taatcaaatc ttttcgagta gctgttttca ttatagcacc ttccttactt
     2701 tttttcattt atttctatca gctgcttttg tcaaaagcac ataggaattt cacctctccc
     2761 caagtttacg gcgagcctta cggaagtatc attatcccaa ctttaaatca tcgcttttta
     2821 ttgactataa aaattatttt agaatattta tcgctccttc tttacagcaa gaaatcatgg
     2881 cgcaactaaa atatgctcct taaagtgtta acgtactgat agaatctatt taattttcaa
     2941 tgaacaatag gttttctacc taaaagaatc tactaattcc ttaactatta gaagattctt
     3001 ttaaacagaa aaagtggaaa ggggattttt ttaattagtt aattttaaat tttaagatcg
     3061 cttctattaa tttaatttct agctgactta tcatatattc atctacatag gtaacgaatt
     3121 gacctacctc gttaaatcca tttgtcaaac ataacttagc catataagaa tgataatgtt
     3181 tgagtatcat cttgatcgaa attggatctc cttctagagc agataagata atggatagtg
     3241 gaactaatag gtactgccgc tttttagatt tatctaccat aggtcatacc ccattttcaa
     3301 ttgttcaact gccttctttc gttgatacca gataccactt gtgctcatat tgagaatttt
     3361 accgatttca gcatcattaa aacctgcaaa ataataaagt aggataatct ttcttcttat
     3421 ctccgataac tgatcaattg ctgctgataa cttttgatct gaaattaata tttccattcc
     3481 aaataagaaa tagacatctt gttcatcaaa atgagtttgg gaatacctgc cttcatttga
     3541 atctgccaat ggttcctcat tatcctttat gttagcgttt cgttttcgaa tagacttagc
     3601 ttcgtttcgt aatacttttt tacagtaggc atcaaattgg tgttgtatag atatgtcatt
     3661 gtaatgttgt tgcatgataa tatgctcctt tttccccctt tccactaata caacaatcag
     3721 aaagcccctt tttttcgaaa aaactagaaa aaaaacataa aaatatttat cattctttaa
     3781 acagaaaatc tttctat
//
LOCUS       DAFGZE010000145         3679 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.149, whole genome shotgun sequence.
ACCESSION   DAFGZE010000145 DAFGZE010000000
VERSION     DAFGZE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.149"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..275
                     /locus_tag="LTU54_002961"
     CDS             <1..275
                     /locus_tag="LTU54_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBL8297593.1"
                     /translation="LIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERINE
                     LIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            418..639
                     /locus_tag="LTU54_002962"
     CDS             418..639
                     /locus_tag="LTU54_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297594.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            655..1497
                     /locus_tag="LTU54_002963"
     CDS             655..1497
                     /locus_tag="LTU54_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297595.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1560..3362
                     /locus_tag="LTU54_002964"
     CDS             1560..3362
                     /locus_tag="LTU54_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HBL8297596.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3421..>3679)
                     /locus_tag="LTU54_002965"
     CDS             complement(3421..>3679)
                     /locus_tag="LTU54_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL8297597.1"
                     /translation="GITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQN
                     FNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1311 a          522 c          749 g         1097 t
ORIGIN      
        1 aactcatcaa gctggctaat aagggtcaag attatgaaca tctagcagat gagattgatg
       61 agctgagaga caagcgacag ctccttttag tagaagatgc ctccctcagt ggcgagaacg
      121 agcgaatcaa tgagctgatt gaatttatcc gcaagaacaa attccgtacc ttagagtacg
      181 atgataagct tgtaaggaag ataatccaga gcgttacagt ctatgaagaa cacttcgtca
      241 tatccttcaa atctggcatc gaaatggaaa tatgaaaacc agaagtaaat agcccatgat
      301 tctacagtag agtcgtgggt tttcttttgc ttgctgatta taataatatt ttaataacta
      361 ttgttattat aaaccaaatg atttataata ttcttagcgt aaacttggag gtgctgtatg
      421 actacggcag aaatgattaa agaactgtgt gagcaaatga atataagtgt ttccgaactt
      481 gctagacgta ttggacagac tccacagaat ttcaataaga aattacaacg agaaacggta
      541 accttagatg agttgaaagc gattgctgat gtgctaggtg tcaagtttgt gcaggctttt
      601 atattgcctg atggcaatga aataaagata ggtaattagg aggtgtaatt tgtattgaaa
      661 atcataacaa taaatgatgt tgagtatgct gtatttgctg ctaatgaagg tacttccaaa
      721 ccacaacctc atatcataga aacgaaatct ggtactattc cagaaggaaa acaactgagt
      781 ttattaaaag aatatcttaa gcagaatgat atttccccaa taaaaggagc aacaacatat
      841 tggtgcattg acaaggttct taagcttgat tcaagtaaag aaaaaactat aagtgaaaca
      901 atccataaac aaaaatactt atctttaact gaagaaaata ttgagaaaca gcataagttt
      961 gtaggtgcaa gctctaatta tgggaaagaa ggtctaataa tacatgatgt tttgaatgca
     1021 tttcctcttc ataatgattt aaatactatt gcgatgaaga ttgcggtaat tgatgtaaca
     1081 aattcaacac atctttctca atataagagc aggttgtctc tatatgattt ggctaaagta
     1141 attctggaga ttccaaattt tgatgatcgg ttggcaaaag gagatcctca gttgataaat
     1201 attatcgcaa ggaacattgg tgcagttaat atgttctcat ttgcatccaa gtactgtaca
     1261 tatcataatg tggaagtgtg tgggagagat gattactcca tctttgatgg tatcgttaaa
     1321 aatactcttc cacactatat ccaaggacta actacgaata agattgatac gtggcgaaga
     1381 agttttgatt acgaggcatt taacgaatgt gttggaaaac tacttgatga aaataatata
     1441 catattcctt ttagaagaag aaagttagat cacttcttgt ggtatgcgaa taggtagaat
     1501 ataagaaaaa ggcatcgtaa aagtgattct ggataaaatt aatgggggag gaaataatag
     1561 tgggattatt tgattttcta aagaaaaaac aaggaaaggt tagtaaacac gttgattatc
     1621 aactggcttc tagtctattt cgtactggta aagtttttgc tgaaaaattg gaagtggaca
     1681 tatcaaatgt agatgagttt cgtaaaactt ttatcgcttt tgatgtggaa acaactggcc
     1741 taaattcttc ttcagataga attgtagaga ttggtgcggt gctatttaaa gaaggagttc
     1801 caattcaatc attcggaact cttgtcaatc caaaagttag aattgcagcc tcagcttcgg
     1861 caataaataa tataactaat gaaatgctaa actctgctcc aaatgagcag gaagtttacc
     1921 cccagttggt agaattttta ggagatgcac tagatggaaa aatcactatg tgtgcacaca
     1981 atgcaagttt cgattttgga tttttaagta acacattttc tagacttggg tatgatgcta
     2041 aaattaagta tgttgatact ctgagtctct ctagaaaaca tataaaaggc attgtaaatc
     2101 ataagcaaga gactgtagcg gaatatttcg gattaataaa tgacactgcc catagagcgg
     2161 aatcagatgc agatatatgc ggaaagattt tttggggtgt tttagatatt attgaggagt
     2221 cgttggaaga acaaaaaaag caaatagaaa agatgactcc agacataaaa gaacttgaag
     2281 cttgtgcttt tattcaagat attattgaga aaaaaggatg cgacaccagt tggatcaggt
     2341 atcgaaaaaa cagtaataat tatgtcgatg taacttgtct atatacattc ttaaagttta
     2401 agtttgctaa gaagggaaat tacatcatta ttagaaaaga tgctacaaat ggaattgatt
     2461 tacctataga accttgtact gtaagtgaag gcggtacaac aaatgtgcga gtctattttc
     2521 gtagcccctt tgacttaacg cctctttcaa attatatttt cagtgcttat agcgattgtt
     2581 ataattctat gaatgaatat atgagtgtta gtaattacac taaaaaagaa gcagagcaaa
     2641 gccttagtat gctgaaatct atttcaaaat cagcgatgaa agagttatta ttcgatgcaa
     2701 aaaatcgtgg atatgatgag actgaagtta acctaaatat tgaaaaagtg atatctagag
     2761 aagatgtaat tattaacggg ttgcataatc gagtttcatt aaacaagata cgaaatatag
     2821 ggaattggga gaaaggattt gatgcaggtt atccatattg ggaacgtgga gaaactgcta
     2881 ggaaaaatgg caaagtagat gaagctattg cactttttga taaagctcga tataacggct
     2941 atgatgctcc tgcgctatat gattcttatg ctaaagctta tcgaaaaatt aaggattacg
     3001 ataatgaaat tctcattctt gatgaaggaa ttatgcgtaa aacacggcac gatgttggta
     3061 ctttagaggc tcgtagggat aaggcaatta agctattatt tgctaaacaa gaggcagagc
     3121 ggatagctaa ggaaaaatct gattttctaa aagcgaacaa aaaagaggat gcgattagtg
     3181 agcctagcaa gaagcgtgga agacagatta ttcaaatgac tgatgatgga acaataataa
     3241 aagaattcga tactatagct tctgctgtaa aagaagttgg cgttaactca aaaagtatta
     3301 gggatgctgc aaatggagtt caaaaacatg ctggaggata ctactggaaa tacaaagatt
     3361 agcagataat ctaagataac ctaggatctt cttaatagat acccgtggtg ctagttaatt
     3421 ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag
     3481 aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga
     3541 gttgaaattt tgacaaccta atttttctaa ggaagaaaaa acagtcccaa ctgtttttct
     3601 ttgttttcca agtaagctgt ggtctagttt ttctgattta tccatgtttt tacgtggcgg
     3661 aatggatata gtaatgccc
//
LOCUS       DAFGZE010000146         3556 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.150, whole genome shotgun sequence.
ACCESSION   DAFGZE010000146 DAFGZE010000000
VERSION     DAFGZE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.150"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            28..228
                     /locus_tag="LTU54_002966"
     CDS             28..228
                     /locus_tag="LTU54_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297598.1"
                     /translation="MLKRIAWDKDNKTVIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            419..1588
                     /locus_tag="LTU54_002967"
     CDS             419..1588
                     /locus_tag="LTU54_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297599.1"
                     /translation="MTKNIEVYVDGSQEERDSFFDFFWKNSREYVKALNLVYKQLMFE
                     ELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKLDKAKAELSKLRNEKNKEAR
                     EILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASKDFGNDFIGIVTGQRTPRIY
                     RNKSKYGLLPFRGRDIKIHKADKFYISLPKGFRLKVNLGSKPRKARDVASVLQHIMDG
                     DYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNELIVNFADLCSGECLINDKSV
                     MSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRKLDQDRWEAIKKRESNFVKT
                     YNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLKEQLVNKGKRYGIEVRS"
     gene            2513..>3556
                     /locus_tag="LTU54_002968"
     CDS             2513..>3556
                     /locus_tag="LTU54_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL8297600.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRANDLPYPLPKELYIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSETRSERRRELAKIIKDEIEKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCL"
BASE COUNT         1403 a          440 c          674 g         1039 t
ORIGIN      
        1 ttattacatg aaggattaag tggtaatatg ttaaaaagaa ttgcatggga taaagataat
       61 aaaacagtta ttgagcttaa agaatataaa tacaattatt tctcgagatg tatagataga
      121 aagttactgt ttaaaaaaga aggattagaa tttagttttg atgaagatga atttgaaaaa
      181 ttagtgatgg actggcgtaa gaatttactg gatacggtac aattctaaaa gcaattgagg
      241 tcgaaattgc gatacttgaa tcatggtcta tattatcgtg ttatgagtga aatttctaat
      301 aaagataaga tatttcattt ggacagtcat gtgaatatga gcttaacaat taaacgtatt
      361 tatgatcttt tatatgttta taaacattaa tgaggtgcta aaaatgacaa caataactat
      421 gacaaaaaat atagaagtat atgttgatgg atcacaagaa gaaagggatt ccttttttga
      481 ttttttttgg aaaaatagta gagaatatgt aaaagcgttg aatctggtat ataaacagct
      541 aatgtttgaa gagttgataa aagatgcaat taaagaaatg gatcaaggtt atgctgagag
      601 agaagaaagt attcaaaatc ggattgaaaa gttagcatat actgatccaa tagatcaaaa
      661 aaaacttgat aaagcaaaag ctgaattatc taaattgaga aatgaaaaaa ataaagaagc
      721 gagagaaata ttaacgcaag cgatagggtt gaagagtcaa acgagaacac gagatgtagc
      781 aaaaaaatta gactttgact ttggagattt tgttgattca gcaaatatga aagctagtaa
      841 ggattttggc aatgatttta ttggaattgt tactggtcaa agaacaccta gaatctatag
      901 aaataaaagt aaatacggat tgttaccgtt cagaggtcgg gatattaaga ttcacaaagc
      961 tgacaagttt tatatctctt tacctaaagg atttagactt aaagtaaatt taggtagcaa
     1021 gccaagaaaa gcaagagatg ttgcttcagt tttacaacac attatggatg gcgattacaa
     1081 attaaatcaa agtaatctta tttttgatgg aagaaaatta ttttttaatc taacaatttc
     1141 ttttgaaaaa agctataatg aaatggaatt caagaaaggt aatgaattga tcgtaaactt
     1201 tgcagatttg tgtagcggtg aatgtttaat taatgataaa tctgtaatgt cttttggtga
     1261 tagccaatat ataagaaact tcaaaagtaa aattttagca atgaaatcaa aagaacaaag
     1321 ccgaggagtc ttttctaagg gcggtcatgg tcgaaaaaga aaattagatc aagatcgatg
     1381 ggaagcaata aagaaaagag aaagtaactt tgttaaaact tataataatc aatgttctgc
     1441 aaaaatcgtt aaaactgcta ttcgcaataa agctcaaatt attcgtatca ttgaaccaga
     1501 taaggcttat ggcgaatggg cttactatca attgaaagaa caactagtaa acaaaggaaa
     1561 aagatatggg atagaagtca gaagttagta atttaaacaa tcgttgattt ggcaacgaaa
     1621 tagtgaggga aaatccactc actgaagggt aaactccgtc aagttgtgtg accagacgga
     1681 aggacgaatg ttgttgaggt tttagaacta tataaaacag catgtttctc aaattatgag
     1741 atgttaagaa aaatgattcg agaggtttta gaactatata aaacagcatg tttctcaaac
     1801 aaagtgcaga acgaaaatca attcatcaca gttttagaac tatataaaac agcatggttt
     1861 taaaacaaga aataagtcag ttttcctttt gctttagtaa ggaaaaatag agtattctca
     1921 ataaaactta aaataagaaa gaataagcaa acctttgttt taatagctaa tcgaagtaaa
     1981 acccaaagcc atatgaactc ctgaatcagc gaaagtttat gatgattggg cttactaata
     2041 atttaggaat tagatggtaa atctaaatta aacaatcgtt gatttggcaa cgaaatagtg
     2101 agggaaaatc cactcactga agggtaaact ccgtcaagtt gtgtgaccag acggaaggac
     2161 gaatgttgtt gaggtttaga accacataaa acagcatgtt tctcaaattg ccttagtggt
     2221 tgaattgaga tctatcacgt tttagaacca tataaaacaa catagttctc aaattgttta
     2281 aaatgattgt caagttaaaa aaatctggga tcatataaaa tagtatagtt ctcaaatatg
     2341 attagaaacc ctattaaaga gcaatattaa accaacttac ttaaatcgtt taattaagaa
     2401 cagtaatctc ttttagtcag acttttagtc tgactttttt gtatttccaa aatttattct
     2461 ttacaaaagc gaacgtacgt ttgtataatt gcttgtgaat gggggaataa aaatgtttga
     2521 ttacagtaga gaaccggtga atgactattt tttaatcgac atgaagtctt tctatgctag
     2581 cgtggaatgt attgaacgaa atttagaccc aatgactaca ccgttaattg tcatgagtag
     2641 atcggataac actggttcag gtctgactct agcttcttca cctactgcaa agaaaaaata
     2701 tcatatcact aatgttagta gagctaatga tttaccttat ccattaccaa aagaacttta
     2761 tattgttcca cctcgaatga acttatatat aaaaaaaaat atggaagtta atcagatttt
     2821 tagaaagtat gtcgcagatg aagatttatt gatttattcg attgatgaat caattttaaa
     2881 agtgacaaaa tccttaaatt tatttactag ttcagaaaca agaagcgaga ggagaagaga
     2941 attagctaaa ataatcaaag atgagattga gaaagaaaca ggactaatag caacagtcgg
     3001 aataggagac aacccattat tagctaaact cgcactggac aatgaagcga agagtagtaa
     3061 agaatttata gctgagtgga cttatgagac tgtacctgaa aaaatatgga aaataaacga
     3121 gatgactgac ttttggggca ttggatcacg catgaaaaaa agattgaatc atatgggaat
     3181 tatgagtata gaagatttag caaattggaa tccctacaca attaaaagtc agttaggtgt
     3241 aatcggactt cagttatatt tccatgccaa tggaattgat cgtacggata ttgctattcc
     3301 accagaacca accaaagaaa agtcttatgg gaattcacaa gttctaccaa aagattatac
     3361 gaggagagaa gaagtagaac tagtagtcaa ggagatggca gagcaagtag ccattcgaat
     3421 tagacaacat aattgcaaaa ctggttgtgt acgcttagca attggtacgt caattttgga
     3481 agaaaaatca ggtttctccc accagatgaa aataccaatt acagataata cgaaagaatt
     3541 acaaaattat tgtctt
//
LOCUS       DAFGZE010000147         3533 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.151, whole genome shotgun sequence.
ACCESSION   DAFGZE010000147 DAFGZE010000000
VERSION     DAFGZE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.151"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..174
                     /locus_tag="LTU54_002969"
     CDS             <1..174
                     /locus_tag="LTU54_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297601.1"
                     /translation="SGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            167..307
                     /locus_tag="LTU54_002970"
     CDS             167..307
                     /locus_tag="LTU54_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL8297602.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            377..796
                     /locus_tag="LTU54_002971"
     CDS             377..796
                     /locus_tag="LTU54_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBL8297603.1"
                     /translation="MEKYLNTLSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVDVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            793..1803
                     /locus_tag="LTU54_002972"
     CDS             793..1803
                     /locus_tag="LTU54_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL8297604.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYSINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSANKYGIPLTLDTPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTL
                     SDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPD
                     VNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIP
                     KR"
     gene            1918..3126
                     /locus_tag="LTU54_002973"
     CDS             1918..3126
                     /locus_tag="LTU54_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297605.1"
                     /translation="MTAEIGIMNKSGIVLASDSASTIGDSKVYNTAKKLFTLDSMHSV
                     GIMIYGNAEFNGIPWEIIITQYKKSIGSSVFNTLEEYADNFIEFVKTASFIRSEQTEQ
                     EQMIGVFQKIISGLFESIEVDINFLISQGTQIDKDVLVKLLQTKMNTNLSQQSQTFIL
                     DIEKALFLSNYGEILKDILNSISTMEGVSEAISEEIQSYVYEIIIRDDVYSSPTGIVI
                     AGYGRMDIFPKLYSYNMFGFVMNILKYSEYESAQIGNDNGSLRSTILPFAQSDVVNTV
                     VQGVDPQITNYLSSQVDSFDDNGKNTYINIIKNISEFQQNQFIFPLLNMIALLPVEET
                     AIIAETLLNLTSFKRKYTTSVETVGGPIDVLAITPNDGPIWIKRKHYFDIDNNIGYRL
                     RKERANDYNN"
     gene            3110..3280
                     /locus_tag="LTU54_002974"
     CDS             3110..3280
                     /locus_tag="LTU54_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297606.1"
                     /translation="MITIIKPQEKSPVLKFNESNLAHNKMSITDTVFDVMVEQQKKIN
                     NNNRQRKEINQY"
BASE COUNT         1293 a          507 c          668 g         1065 t
ORIGIN      
        1 agtggcacag cggttgacga tattgaagat gtcgatcagt tgaagcaatt aaatgctcta
       61 ctgacaaaac aattggcgat atcggtacag gaacaagaaa aaatgcaaca aatgttagct
      121 caattgacga tggaagtcgc aagtattaag aatggaggga aaagtaatga atagttttcc
      181 gggctttgaa aatataaaac aattttatga ttggggatgt tatacggacc aagatttact
      241 tgactatgta aacatgaact gtttaacaaa agaccaatat aagcagatta ctggaaatga
      301 aatttaatta agcacaaagt taaataaaaa gcgtactcaa acgagtgcgc ttctttgcat
      361 aaaggagaaa tagatattgg aaaaatattt aaatacactc tcagtaatga caggggtgat
      421 aggcgggaca attgttggtt tattgggagg gatggacaat atactacatg ttttaatttt
      481 tcttgtgggt gtggacttct taactggact tgctaaggca tggaaattga aagaaataag
      541 tagtgagata ggtttcgaag gattgttgaa gaaagtctta atcttcgttg tgattgccgt
      601 tgcggttgac gtacagaaaa tcgttgggaa ttctatacct ctaagagaaa tagttattat
      661 gttctatgtt gcaaatgaag ggattagttt tttagaaaac atttcggtat tccttccttt
      721 gccagataag ttaaaagagg tattccaaca aatacgaaat gatactgaaa ataaagaccg
      781 aggaggaacc aaatgaaaaa gaaaatcttt gtaggagcta ttatagctct ttttttattg
      841 ccaataaacg cctttgctta ctcgatcaac aatgaattca atttgggcgc aaacgaaggt
      901 agttctcaag tagcaaataa ccaatacatc ctattgcatg aaacagcaaa cgaaactgcg
      961 acaggacgaa atgaagcgca gtacatgaaa cgttcttggt acaatgccta tacagcgtat
     1021 attgttggcg atggtgggat cgtttaccaa gttggacaac ctggttatgt acaatatggc
     1081 gctggttcat atgctaatgc aaatagtcct gtgcaaatcg aactgcaaca tacgcacgat
     1141 aaagtaacgt ttgaaaagaa ctacaaggca tatgttgaat tagcgagaga ttccgctaat
     1201 aaatacggta ttccacttac attagacaca ccgtataatc aaccaggaat caaatcacat
     1261 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
     1321 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
     1381 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
     1441 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
     1501 aacttacacg tcgctggatg gcacatcgct aactataaat acgagtatat tttcattatg
     1561 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
     1621 gtaaatcaag cttataatac ttctggaaac gttggctatc atgtatcttt taacatgcgc
     1681 aatttcccta ataagaaagt atacgtcatg atgcgggcaa cgaatgatcc agaaggaaac
     1741 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
     1801 taaaaatagc tcctcgttga ggagcagtac atagaattga aaattaacgt taaatcaaaa
     1861 aaatatttac aagatgttta tttaagtaga taataaaaga aaaaagcagg tgaaattgtg
     1921 acagcagaaa ttggtattat gaataaaagt ggaatcgtgt tagcttcaga ttcagcatct
     1981 acgattggag atagcaaagt atataatact gcaaaaaaat tatttacttt ggattccatg
     2041 cattctgtag gtatcatgat atatggtaat gctgaattta atggtattcc ttgggaaata
     2101 atcattactc aatataagaa aagcattggt agttctgttt ttaatacttt ggaagaatac
     2161 gcggataatt ttatagaatt tgtcaaaaca gcatctttta ttagaagtga acagaccgag
     2221 caagaacaaa tgattggggt ttttcaaaaa ataatttcag gtttatttga gagtatagaa
     2281 gtagatatta actttttaat aagccaaggt acacaaatag acaaagatgt cctagtgaag
     2341 ttattacaaa caaaaatgaa taccaatttg tctcaacagt cccagacatt cattttagat
     2401 attgaaaagg cgttatttct tagtaactat ggagaaatat taaaagatat tcttaatagt
     2461 atttctacaa tggaaggtgt ttcagaagct atatcagagg aaattcaaag ttacgtttat
     2521 gaaattataa ttagagacga tgtttattct tcccctacag gaatagttat agcaggatat
     2581 ggaagaatgg atattttccc taaactatat tcttataata tgtttggatt tgttatgaat
     2641 attctgaaat actcagaata tgaaagtgct caaataggaa atgacaatgg ttcattaaga
     2701 tctactatat taccatttgc tcagtctgat gtggtaaata ctgtagttca aggggtagat
     2761 ccacaaatta ctaattatct gtcgagtcaa gtcgatagtt ttgacgataa cggaaaaaat
     2821 acctatatta atattataaa gaatatttca gagtttcaac aaaatcaatt catatttccg
     2881 ttactaaaca tgattgcttt actcccagtt gaagaaacag caattatcgc agagacttta
     2941 ttaaatttaa ccagttttaa acgaaaatat actacttctg tagaaacagt aggtggccca
     3001 attgatgttt tagctattac acctaatgat ggccctattt ggattaaaag aaaacattat
     3061 tttgatattg ataataatat tggatacaga ttgcgaaagg agagagcgaa tgattacaat
     3121 aattaaacct caggaaaaat cccctgtttt gaaatttaat gagagcaatc tagcccataa
     3181 taaaatgagt ataactgata cagtatttga tgtaatggtt gagcaacaaa aaaaaattaa
     3241 taataacaat agacaaagaa aagaaattaa ccaatattaa tgttttttct gttgcgtttt
     3301 ttgcagtcat aatttaaaac ctcgctcaat cttgggcgag gtcttttttt atttttctga
     3361 aaagttaaag taaataatag cataaaataa tatattttac aaagaataag tacaatctag
     3421 ttttttgcta ttaaatgtgt aataataaat atgccatcac aacatgaaga atgaaatcca
     3481 ttattatcta gtctagtcca ttctttttgt ttgcagtatt tgtgatggct ttc
//
LOCUS       DAFGZE010000148         3516 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.152, whole genome shotgun sequence.
ACCESSION   DAFGZE010000148 DAFGZE010000000
VERSION     DAFGZE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.152"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..942
                     /locus_tag="LTU54_002975"
     CDS             <1..942
                     /locus_tag="LTU54_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL8297607.1"
                     /translation="LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIK
                     GAHFFDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGI
                     ILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFM
                     APKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMI
                     FIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILH
                     ILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            955..1962
                     /locus_tag="LTU54_002976"
     CDS             955..1962
                     /locus_tag="LTU54_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8297608.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1974..2978
                     /gene="neuB"
                     /locus_tag="LTU54_002977"
     CDS             1974..2978
                     /gene="neuB"
                     /locus_tag="LTU54_002977"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HBL8297609.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2975..>3516
                     /gene="neuC"
                     /locus_tag="LTU54_002978"
     CDS             2975..>3516
                     /gene="neuC"
                     /locus_tag="LTU54_002978"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HBL8297610.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTIL"
BASE COUNT         1250 a          445 c          671 g         1150 t
ORIGIN      
        1 ttgaatattg caatctctgt agctttgatg cagacattat attttgatga taaatatgtt
       61 ggcagagtag tcggcggaac actgtcattt gtgttgatag gtttagtaat ttatttaaga
      121 ataatgataa agggtgctca tttttttgat aaaaaacagt ggagatacgc tttaaaaatc
      181 tctttgccgc taatcccaca ttccttagca aatatagttt tagcacaatt cgatagaatt
      241 atgattaatt cttatttagg tcctgctgcg gggggaatat attcctacat tagtaacata
      301 ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc atggttctat
      361 ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta ttatttagta
      421 gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc aaaatttatg
      481 gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt aggctatttc
      541 tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa aacatcgttt
      601 attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat gattttcata
      661 cctaggttag gaataattgc tgctggatac acaaccgtat ttacgtattt tttacttttt
      721 atttttcatt ataatatcgc taaaaaaatt acgaataaac aattatttga cacaaaaaaa
      781 atatgtatca taataatttt tgtatcaatt ttttctttga tacttcatat tttaatcaat
      841 caaatgttga ttcgttacgt tttgacatca ttattgataa ttagtttagt tatatatttt
      901 ttaaagatta agaatcgaat tatagatgac tataattcat aggaggagat ataagtgttt
      961 aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga aattgttcgt
     1021 cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga agaaaaacaa
     1081 ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat tggtgatatc
     1141 agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt tcatgctgcc
     1201 gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa aacaaatgtg
     1261 atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca ttttgtaggt
     1321 atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa gttattgagt
     1381 gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat attttcttgt
     1441 gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt tagtaaacaa
     1501 ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt tatgatgtca
     1561 ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt tggagaaaca
     1621 tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt aataaaaata
     1681 tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg tatcaggcca
     1741 ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc atatgaaaat
     1801 gataaaatgt atatcattcc accacactat catgctactg acgaactgta caaagattat
     1861 gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag tggacaggaa
     1921 tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt atgatgaaat
     1981 cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta gatttagctt
     2041 tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt caaacattta
     2101 aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag gataatctta
     2161 aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa tctgatttta
     2221 ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact ccttttgatg
     2281 aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt ggctcaggag
     2341 atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca gttttattat
     2401 ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc atagatagtc
     2461 cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat attaatttat
     2521 tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc gatcattcat
     2581 tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt gagaaacatt
     2641 ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat cctcaggaat
     2701 ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat ggtgttaagc
     2761 gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta gtggtcactg
     2821 agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa cgtcctggaa
     2881 atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa agaccaataa
     2941 gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta tagtaagtgg
     3001 aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac aagataattg
     3061 ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat ttactaaaga
     3121 attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt ttttagagaa
     3181 caataaggga aacatggctc ggtctgtggc attagagata aatggtttca caaatatatt
     3241 tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa tgttggcagc
     3301 tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag gcgaagtgtc
     3361 tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg ttcattttgc
     3421 tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt ggagagtttt
     3481 tcccgtcgga gctcctagaa tagatactat tttaaa
//
LOCUS       DAFGZE010000149         3493 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.153, whole genome shotgun sequence.
ACCESSION   DAFGZE010000149 DAFGZE010000000
VERSION     DAFGZE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.153"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(727..1767)
                     /locus_tag="LTU54_002979"
     CDS             complement(727..1767)
                     /locus_tag="LTU54_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL8297611.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2116..2442)
                     /locus_tag="LTU54_002980"
     CDS             complement(2116..2442)
                     /locus_tag="LTU54_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297612.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2429..3232)
                     /locus_tag="LTU54_002981"
     CDS             complement(2429..3232)
                     /locus_tag="LTU54_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL8297613.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
BASE COUNT         1070 a          582 c          498 g         1343 t
ORIGIN      
        1 caaacatgcg ttccctctaa tttcagcgtt cgtcatacgg caaagagccc cacggacatt
       61 tttataacat tatgcttagg catagattca tcgtaatatc acgtattttt aaccttaaca
      121 cagccaaaaa aagtggtatc acttttgaat atctgttata ctaaatccgt tacgggtaat
      181 accgtaacac tgaaaggtaa acgcattaag gggtatagac ttaatgccac ctgtggacgt
      241 cgacgaaggt taccagcctt ccgagtctca cagggccttt cttttttttg tattttcaat
      301 tgtccaaatt tatcgtccaa aagtgcagaa cgaaaatcaa ttcatcacaa gcatagcaga
      361 caattaaatt taaaacaact aaaaatatac aaaaaagtgt aattgaatat tagcagcact
      421 gcctgaaaag aagtaatatc aggcttttca gcttggggaa ataagtgaat tacctctctt
      481 catgaattat tataactcaa aaaagcacat atgcaaacac tagttcccta tttttttgag
      541 tgaaaaataa ctaaaatatc taacgattat gaatttaaaa cctacgattg tgattgtttt
      601 cataaacttt taatctaaaa actccggaat actaactatt tatttgtaat atttgtaata
      661 aaattaaaat cataaaaaag ttctaattta aaattgatca tttttatagt aattgctttt
      721 tatcaactac tctaaccaat tttccgtact aactgttggt aattcgttaa atgtttctct
      781 ttgttcgttt gcaagctttg ccgcaatagc tttttcgaca aaccaatttt ttacataaac
      841 gaaaattaga ttctgcaaat tgtttacttt ttcagttttt tgtttttgat aagctttcag
      901 taaagtcata tataatcctt tgtcagcatc aattccataa gattccagtt cttcataaac
      961 gacaatagtt tgctctaatt cttcagcttt tttctttgca ttatgaatag tttggacggt
     1021 ttgttgagct tcactgtaac ttgaagaaaa tgtttttatc aagtttaata cattttctgg
     1081 tacaaatgta tttatactgt cctcttgcat gatgtcgacg aagttatcta gcaaaacttg
     1141 attttgtaac tggtcagttt cagtgtctat tatgtgtcta ttagtgtcta tagaattaga
     1201 atagaataga ttgtctgcga ttttcgcaat tacggttgag tccttgatat gactggattt
     1261 tgccgtattt gcgattttcg caattacggt agaatcattg atttttcgcg gttttgccgt
     1321 atttgcgatt ttcgcaatta cggtggaatc agtattgtct tcctcttcca tgattttaaa
     1381 gacgtcttga ttcgtaactt ctggcttgcc taagtacaat aaattaggca tagattcaat
     1441 cttaccatca cgtttattta ctcttcctct cttctgtttc agaagacctg ctttttctaa
     1501 ttcttttttg atcttagata ctttaccttc gcggcattgt aacaatttca ttaattcttc
     1561 aactgtgaat atgaagaata tattatcatc agaatctacc cagttatttt tgactgaata
     1621 atcaaatcga tctttaagta gcatataagc aatttttgca tcatttgaaa gatccatgta
     1681 tttttcgtta gtaaaaaata ccttaggcat ttgataatat ttttcacgat aaatttcttt
     1741 tgatttgtaa taattaaaat cattcatagc caatcctctt tctgtaggat agcgttttgt
     1801 tcaaaactat gttacaatga attaaacaaa tatatctatc cactatatat ttttctactg
     1861 ctgataagtc gccaaacttt gttaaagcag tagtcttttt ttatcttttt ttagtttacc
     1921 ttaaaaaata aattaaagca aaccgctcta tttcagggtt taagctcatg gtaactatat
     1981 cacgatatta caataacatt atatcacgat atcgtaatat tacaatatga agataagcga
     2041 cttatcagct ttgatactac tgatttttat aaaaaaaata tcacgatatt atgatatcgt
     2101 gatattgaat gtaaattaaa ttttattttg tttctttgct agtttatatc cttcaatcaa
     2161 gtattcttca ggtatggatt ttaatagcgc atcttctata acgtctggtt tacttttgtc
     2221 tgattcagca atagtcgtcc aatatttgag tgctgacatt agaattggat ttatacgaat
     2281 gctatctgct gttcttacac tacttccttg atcttttatg actttactta tatttttctt
     2341 ggttaattcg atttcttcta cttcttttgg tttgtagttc tttttatttt tatttcctag
     2401 tagtctactt actgcagctt gactgcgatt atcttctgcc atcaaaatca ccaacttctt
     2461 ttaaatattc ttctgtaaat gctttatagt catcttcagc tgttgagggt ttattaaaat
     2521 tgattagatc tgatccagca gcttgcgctt cgtcaacggt tattgttcta cgaatataag
     2581 ttttgaaaat ttttgcacca atgactctag aaatttcggt tgctgcagtc gtcatttctt
     2641 tagaaatgtt ataacgctta ttatacttag taaataggac tcctcgtact tcaagcttgt
     2701 cgttcgtata ttcttttacc gtttcaatag tttcacttaa ctttattagt ccttttaatg
     2761 agaaatacgc agcttcaact ggaatgataa tttcatcaga aacagtaaat gcgttgactg
     2821 taagcattcc taaacttggt ggacaatcaa ttataatata atcatagttg tccttgattg
     2881 gttgaagtac tcttttaagc ctgtgttcac ttcctacttg agtaaattcc ctctctgcag
     2941 aagaaagggc tatgtcggct ggcaaaacat caattccttt atagtccaga atagcatctg
     3001 taatgtcagc ctcccctttt aatacttcat aaattgtagg ttggtttgtt ccatctatat
     3061 cgaaacctac attatctgta gcatttccct gaggatcaat gtccactaaa agtacatttt
     3121 taccgtaaac aaatttcaaa catgctgcta aattaacgct agtcgtagtt ttagaaactc
     3181 cacctttttg attggctact gacaatattc ttgccattaa aatatccccc attcatctta
     3241 tattataata taattatatc ataatattgt gatattgcaa tatcgtatat gcattatatt
     3301 gtaatattgt gtgattattt tgttttaaag atatatatcg gttcggatat aaagattatt
     3361 catatgttat tcgaaagtaa aataatcact ttttattact ttaaaatttt ttattaatca
     3421 taagaaatat gttttgcgag gagagtgtaa aatattttgt gtaaatgaaa aaatccatac
     3481 aaaaaaggaa gtc
//
LOCUS       DAFGZE010000150         3443 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.154, whole genome shotgun sequence.
ACCESSION   DAFGZE010000150 DAFGZE010000000
VERSION     DAFGZE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.154"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            125..1813
                     /locus_tag="LTU54_002982"
     CDS             125..1813
                     /locus_tag="LTU54_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297614.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            1800..3434
                     /locus_tag="LTU54_002983"
     CDS             1800..3434
                     /locus_tag="LTU54_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297615.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
BASE COUNT         1189 a          498 c          561 g         1195 t
ORIGIN      
        1 aataaaaatt gattcttttc tagtatatca tactagtgct atgaaaaatt taacgcaaaa
       61 cttattaaaa aaagacaatt ctagtatact gaaaatagaa ttaaaaaagg agaacaacaa
      121 atctatgaaa ataagtaaaa aagcatttct tttagtagtt cttatcttac ttagtacttt
      181 atattcagta aattttatga gaaatgctca agaaatttat acaacaggtg atttatcttt
      241 tcatctttca cgaatcaaag ggttatcttc tatttttgaa gggccaatca actatacaac
      301 atttaacaat tatggagatg gactgaacta tttttatcct tttcttacaa taattcctgc
      361 agtcgttttt tatggaattt caaataactt aatattaagt tatgttctat atatttggtt
      421 attaaatatt tgtacgattc tgattagttt ttattatgga gaaaagtttt ttaaaaagat
      481 agatgctgct tttttattta gttgtttata tacttttgca acttatcgaa cagttgatat
      541 ctattatcga tccgcaattg cagaagcaat cgctcttacg attatcccag ctgttttata
      601 ttatgcttat ggattgatat tcaatagaga aaaaaaagtc atcgctttat ctgtcagtat
      661 aagtctactt gtgtatactc atgtcttaac tacactattg tgtagtgcat tgattgcttg
      721 tatgattata gttcgtttgt ttttaagcaa gcaaataaaa aacgatataa aatttgtggt
      781 cgtgaattta gtaaaggctg tttttttgac ttgtgcatta actagctatt tttggtatcc
      841 tatgttgcag cagatgaatt atcaagaaat acacagtccc ttcaaaacag atttgcagct
      901 agaagcactt tctatttcag atagtatcat tcaagcgtta aataacgata ttacaacata
      961 cactatgggg ttattaggat tggttgcgtt gatttttcct ttattcatac tgaaagaaat
     1021 ggagaaaaaa gaaaaaatca tctatgttgg cgtagtttct acatgggtat gcgtaactaa
     1081 tttatttcca tggtttttgt ttcaaaaaac accgataagc ttactccagt ttccttggcg
     1141 tatcttaggt atccaaatct ttttttcttc aatgatcata acgcttgttt ttatgaaaaa
     1201 gactatgaat aaaaaatatt catggttaat cattggcgct actgtggtgt ttctactcgt
     1261 cacgtctata gcagcaaaag aaaattacgc aaaagttatc caatcaaaac acggtcatat
     1321 tgttatcaat gaagaaacac tcccacgtta tacaacatca gatatgggag gattatacga
     1381 ttacgcccca acagaagcga taaaagaaag gcctcactta gaaaaacatg aagtaaaagt
     1441 aggagaatcg tgggaaaaag ggaagtataa agctgcagat aataacatta cttacacagt
     1501 ggcaagcaac aaaaagcaaa aagtgacagt accagtatat gcttatttag gtacacaggt
     1561 aaaagtaaac ggtaaagtcg taaatgactc ttataaaaaa catggcttag tgaatgtgga
     1621 tgtaaatgct ggagaaaata agatagaaat tacaacaaaa tatactccaa ctgctctcat
     1681 ggctttatgg atcagtattc tgacttattg tttagtggtg tattcaagtt ttaaacgatt
     1741 tgcacctaat aagttgaaag attctcgata tgtgactaaa aaggagataa acaagaagaa
     1801 tgaaaataaa taaaaaagta gtaatattga gtggattaat tatagttagt agtatatatt
     1861 ctttcatctt tttcaaaaat acacagagta tttataccac tggagatctt tcttttcact
     1921 tatctcgaat caaggggtta tcgactattt ttaccagtcc gattaattat gagacattca
     1981 attatactgg atatggtgta aattattttt atccctttct gacttttttt ccagctgtaa
     2041 tgttatattg gatgacaaaa aatttgattg taagctatat tatttacgtt tggttattga
     2101 acttgtgtac aacactcgta gcttatcatt atggcgagcg gtttttaaaa caaaaaaaag
     2161 cggcattttt atttagttgt ctctatattt tctctgctta tcggacagta gacatctact
     2221 atcgttccgc aattgccgaa gctatcgcaa taacacttgt tatccctgta ttattttatg
     2281 cttatcaaat catctctgga aaagaagaaa agtatccgtc tgtaaagcta gcattgagta
     2341 tgagtttgct agtgtacagc catgtactat caacattaat gagtacagca ttaattatca
     2401 tttttatttt tattcgtttg gtttctaaag gtttcaagaa tgcagatttt attgctatat
     2461 ttaaaaaatt atttagtgcg gcaggaatga cattggttct gacgagtgct ttttggtatc
     2521 cgatgtttga acaaatgctc taccaaaaaa tcaacaaacc tagcgtgaca aatttatatg
     2581 ctcatgcatc taatgtgttc gatagtctga ctgaagctat gaacaacgac ttaactactt
     2641 actcaatggg actagttggt ctgttatctt tatgtattcc cctgatttta tttaaaaaat
     2701 taacaaacat agaaaaaaag atttattatg gtacgtgttt gacttggcta gctacaacta
     2761 gtttggtgcc atggtatctt ctgcaaaata cgccagccaa attgttacaa tttccttggc
     2821 ggatcttaag cttgcaaata atattctctt ctctgatatt aacgatgata tttttcaaaa
     2881 atagaagata caataagaca agagaactat tttatttatt tatgactgtt ttgttattaa
     2941 tcactttaac tgtttctagt aaaaaaaatt tcaatcaaaa aataatttct cagccgggac
     3001 atattgttgt aaacaaagaa acagtagagg cgtataccac taaaggaatg gattattttt
     3061 tatatgatta ttctcctatc gcttcattgg agaataagga gcatataata aaacacgaag
     3121 caaagattgg cagtcaatgg gtaaaccttc cttataaaac aactggtagt tctatcatat
     3181 atagagtaac taatgagaag agccaagtaa tcacacttcc tgtatatgct tatttaggaa
     3241 cgaatgtaga aataaatcat caacctgtaa aggagcaggt gaatgctaat ggattggtac
     3301 aaataagcgg agtcaaagga gttgctgata ttaaagtaag cacaagttat acagtaacga
     3361 ctattatttc ttatcttgtt acattaactg catatttatg tttatttttt aaaatagttt
     3421 tccataaaag gtaaattaac ttt
//
LOCUS       DAFGZE010000151         3319 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.155, whole genome shotgun sequence.
ACCESSION   DAFGZE010000151 DAFGZE010000000
VERSION     DAFGZE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.155"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..1157)
                     /locus_tag="LTU54_002984"
     CDS             complement(<1..1157)
                     /locus_tag="LTU54_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8297616.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRV"
     gene            complement(1160..>3319)
                     /locus_tag="LTU54_002985"
     CDS             complement(1160..>3319)
                     /locus_tag="LTU54_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBL8297617.1"
                     /translation="ISEDGKLLHLYYGENLPDNDYSHLIEMHHRPMTTYRNENDLLYS
                     LEHLKQEFPEYGTTDFRHPAISLLQKNGSRLSDFVYQGYEVEEGKPKLEGLPATYVDN
                     NDESKTLKVYILDSVTNVQVELLYTIYRDYPVITRSARVMNKGKDIQIIENISSLSLD
                     LPDANYEWQQLSGAWGRERTIKSRTLQQGIQSIESTRGISSHQHNPFVTLKRKNTDEN
                     QGEAIGAALVYSGNFLIQAEVDTWDVTRLQVGINPFGFNWKLNPGEIFTAPEAILVYS
                     SKGLNAMSQTFHQLFRKRLARGKWREKDRPVLINNWEATYFDFDEEKLVNIAKKAFDV
                     GIELFVLDDGWFGERENDCAGLGDWHVNPKRLPRGIGSLSEKIRSFGMKFGLWFEPEM
                     VNKDSELYRTHPNWIISTPNRKPNHGRNQYVLNFGLDEVVENIFDQMCKIIDESNLDY
                     IKWDMNRPLTDVFDSHLSADQQGEVFHRYVLGVYRLYEKLITKYPNILFESCSSGGGR
                     FDPGMLHYAPQAWASDDSDAIERLKIQYGTSMLYPLSSIGAHVSIVPNHQTNRITPIK
                     TRGNVAFFGAFGYELDLNELSEGDLRIVKEQIAFYKKYRTIIHNGTFYRLLSPFDDDN
                     QTAWMVVSHDKKTAIVAHYKTLNEVNAPYRRLKLKGLDNDGLYNVGQNSLRSGMELMR
                     AGLVCSDASSGQINDQNRRPETFDFDSKIWVITQEEK"
BASE COUNT         1076 a          625 c          482 g         1136 t
ORIGIN      
        1 actctagtat tttttttatt tgtcgcaaaa aatgttacaa ataggatcac aataaatatt
       61 cctgcgaata caaaaaatgg agcagtcaaa tttgtacttc ttgttagaaa ttgtgctaat
      121 tgcatagcaa taggagctat aaaattacca atattacagc caataaaaat caatgaagta
      181 gcaaaattaa ttgtattctt tgtagtgact ttatctaagt tactaaagat aaatgggaag
      241 caccatgatc ctgttatact tgataaaaat aaacccgtta gcaggaacgt catattaccg
      301 tttgacacag caattaacaa atttgaaatt ataaagaata taattcccaa gtatagggtt
      361 ccttttccta tccacttatt tattggtcca aacataaatc ctgctagtat tcctaagata
      421 ggcattagag ctaaatagtt actagcatta aagtttaccc ctttaatttc agttgcaatt
      481 gatgcaaatc gtacagaaat agcaatacta ttcattacga gtaagattgc aaatagaact
      541 agggcaaaaa ccattggatt catcttttct tttacatgat tttcaggtat gtcactattt
      601 tcatctctaa tttcaggaac ttttagcgcg aataagagag ctacaggaaa cgcaattgca
      661 tagactaaaa acgaagccgt ccaattaatg ttcactaaaa ctccagcaag aaaaataagg
      721 attgtctgac ctaaagcttc cattgaattt ctaataccta atagcgttgc tctagtattg
      781 ccagagtata aagagctgat atatgtcaca gccaatgaat tatatactcc taaaccggca
      841 cctaatatta ctcgactaat aagaacggtt tggaaggtag ctgtaaatac tggaataatt
      901 cctcctatac cagctaataa taaacctaaa attattgtct ttttcattcc tattcttttg
      961 gcaaagtaac tagataataa gataaacaaa ataacagtta tacttggtgc agttcctaaa
     1021 agttcacttt gactttgtga tatccccaaa gactgtttca tttgtggaat aacaccatta
     1081 attgcttgag agcttgttaa cattagtgaa atacttaaga ttgctccttt ttgaagtaag
     1141 cttttttggt tattcatctc tacttttcct cctgtgttat aacccaaatc ttggaatcaa
     1201 aatcaaatgt ttctgggcgt cgattctgat catttatttg tccactagat gcatctgaac
     1261 agactagtcc tgctctcatc aattccattc cagatcgcaa actattttgt cctacattat
     1321 aaagtccgtc attatctaac ccttttaatt tcagtcggcg gtaaggagca tttacctcat
     1381 ttaatgtttt ataatgagca acaatagccg ttttcttatc atgactaaca accatccacg
     1441 cggtttgatt atcatcatcg aatggtgata atagtctata aaaagtacca ttatgaatta
     1501 tggttctata ttttttataa aaggcaattt gttcttttac aatcctcaaa tcgccttcac
     1561 ttaattcatt taagtcaagc tcatatccaa atgcaccaaa aaaagctaca tttcctctag
     1621 tttttatagg tgttatacgg tttgtttgat gatttggaac aattgataca tgagcaccaa
     1681 tagacgaaag aggatacagc atactagttc catattgaat ttttaacctt tcaattgcat
     1741 ctgaatcatc actagcccaa gcttgaggtg catagtgaag cattccagga tcaaagcgcc
     1801 caccacctga tgaacaagac tcaaatagta tgttaggata ttttgttata agtttttcat
     1861 ataagcgata tacgcctagt acatatctgt ggaatacttc tccttgttga tctgctgaca
     1921 agtgactgtc aaatacatca gtcaatggac gattcatatc ccacttaatg taatctaagt
     1981 ttgattcatc aatgatttta cacatttgat cgaaaatatt ttcaacaacc tcatctaacc
     2041 caaaatttaa tacatattgg ttcctaccat gattaggttt tcgattaggt gtagaaataa
     2101 tccaattcgg atgtgtgcga tataactcac tatccttatt caccatttca ggttcaaacc
     2161 ataaaccaaa tttcatacca aaactacgaa ttttttctga aaggctgccg attcctctag
     2221 gtagtctttt aggatttaca tgccaatctc ctaatcctgc acaatcgttt tctctttctc
     2281 caaaccagcc atcatctaat acaaataatt caataccaac atcaaatgct tttttagcaa
     2341 tatttaccaa cttttcttcg tcaaaatcaa aatatgttgc ttcccaatta ttaattaaga
     2401 caggacggtc tttttctcgc cacttaccac gagctaagcg ttttctaaat agttgatgaa
     2461 aagtttgact catggcattt aaacccttac ttgaataaac cagaatcgct tctggtgctg
     2521 taaatatttc tcctggattg agtttccaat taaatccaaa tggattgatg ccaacttgta
     2581 gtctagtaac atcccatgta tctacttctg cttgaattaa gaagtttcct gagtaaacta
     2641 gtgctgcacc aatagcttct ccttgatttt catcagtatt ttttcttttt aaagtaacaa
     2701 atgggttatg ctgatggctt gaaatacctc gagtagattc gattgattga attccttgtt
     2761 gtaaagtacg cgacttaata gttctttctc taccccaagc accagataat tgttgccatt
     2821 cataatttgc atcgggtaaa tccaatgata aactgctaat attctcgatt atttgaatat
     2881 cttttccttt attcataact cgcgcgctac gagtaataac aggataatct ctataaattg
     2941 tatataataa ttctacttga acatttgtaa ctgaatctaa aatataaact ttcaatgttt
     3001 tgctttcatc attattatca acataggtag caggtaaacc ttctagttta ggttttcctt
     3061 cttccacttc gtacccttga tagacaaaat ctgataagcg agagccattt ttctgtagta
     3121 atgaaatagc aggatgtcta aaatctgttg taccatattc aggaaattct tgttttaagt
     3181 gctctaacga atatagcaaa tcgttttcat ttctataggt tgtcataggc cgatgatgca
     3241 tctcaattag atgactatag tcattatctg gtaagttctc accataatat aaatgcaata
     3301 atttaccatc ttccgatat
//
LOCUS       DAFGZE010000152         3318 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.156, whole genome shotgun sequence.
ACCESSION   DAFGZE010000152 DAFGZE010000000
VERSION     DAFGZE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.156"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..562
                     /locus_tag="LTU54_002986"
     CDS             <1..562
                     /locus_tag="LTU54_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HBL8297618.1"
                     /translation="KFEKKKYVLQVFHPVVTELENIEKQILALINSMEQQSQPIICIL
                     PNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTILKECKFMIGNSSSGIIEAASFK
                     IPVINLGTRQNGREQSKNIINANLNENEIETALNKIQDPQFIKSLKNVKNIYGDGNSS
                     RRITKTLNQILNDGEDIKWIQKQIAY"
     gene            535..1212
                     /locus_tag="LTU54_002987"
     CDS             535..1212
                     /locus_tag="LTU54_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBL8297619.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            1202..2344
                     /locus_tag="LTU54_002988"
     CDS             1202..2344
                     /locus_tag="LTU54_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBL8297620.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2357..>3318
                     /locus_tag="LTU54_002989"
     CDS             2357..>3318
                     /locus_tag="LTU54_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBL8297621.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLIN"
BASE COUNT         1245 a          422 c          627 g         1024 t
ORIGIN      
        1 aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa ccgaactaga
       61 aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat ctcaaccaat
      121 tatttgcatt ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa
      181 atatttaaaa acggatatta tttatattac gaatttagag ccggaggaat acctgactat
      241 attaaaagag tgcaaattta tgatcggaaa ttcttcttct gggataattg aagctgcatc
      301 attcaaaata ccagtaataa atttaggaac cagacaaaat ggacgagaac aatctaaaaa
      361 tattataaat gcaaatctaa atgaaaatga aattgaaact gctttgaata aaatacaaga
      421 tcctcagttt ataaaaagct tgaaaaatgt taagaacatc tacggtgacg gcaacagttc
      481 tagaagaata acaaaaacat tgaatcaaat attgaatgat ggggaagata ttaagtggat
      541 tcaaaaacaa atagcttatt aaaatatact agtgatccat acattattat tggggctggc
      601 ggacacgcaa aagtcattat tgaaacctta agattagaaa ataagttaat atatggattt
      661 tttgatgata agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg
      721 aatagctggg cggatagcaa aaaaaaaatc tataaattta ttgtagctat tggtgactgt
      781 gtagtaagaa aaaagattat aaaaaaattg aatatgccaa gtgagcaata tggaatagct
      841 gttcatccaa gtgctatagt ggcaaaagaa atttcaattg gttatggaac tgttattttt
      901 gcgaatgctg taattaaccc agatgctgta attggagagc atgctataat aaacacaggt
      961 agcattgttg agcatgattg tagaattaat aactatgttc atttatctcc aggtgtatgc
     1021 ttagcgggtg gagtacatgt aggtgtagga actcaaatag gaattggttc tcaatgtatc
     1081 cagatgaaga aaattggatc ttggtgtatg ataggcgcag ggagcactat tgttaaggat
     1141 attccctcac attccttagc ttatggcaat ccagctaaaa ttaagaaaga aggaattaat
     1201 tttgaattct agaattttat tatcaagtcc tcatatggga ggaacagaag aaaaatatgt
     1261 tgctaaggct tttaaagata attggatagc gccattaggt gaaaatgtgg ataaatttga
     1321 agaaagtatt acaaaatata ctggttctaa gggcggagct tctgcgttaa gttcaggtac
     1381 agctgccata catcttgcct taagactgct taatgttgga aaaaacgata ttgtctttgc
     1441 tccttcattt acttttatag ctacagtaaa tcctatttta tatcaagggg ctgtaccagt
     1501 gttcattgac tcagaaccta atacatggaa tatgagccct gttgcactag agagcgcatt
     1561 agaagaagct gaaagaaaca accaattacc taaggcaatc atcgtagtga atttatatgg
     1621 tcaaatggct gattatgaac gaattttagc tattgcaaac caatttaatg ttccagtgat
     1681 agaagatgca gcggaatcgt taggttctac ttataaggga atttcgtcag gcttatttgg
     1741 gacaattggg atctattcat ttaacggtaa taagatcatc acaacctcag gtggaggagc
     1801 tctagtgtct agtgataaaa aatatgttga gcattcaaga tttttagcaa cacaagcaag
     1861 agatcaagca ccgtattatc aacatagcga aataggttat aattaccgtc taagcaatat
     1921 ttctgccggt atcggacgtg gacaaatgga agtattgaat gatagagtag ctaaacgaag
     1981 agaaattttc aatcgatata aggaagcttt ttctggaatt gatagaattg aaatgatgcc
     2041 agaagtggat ggcatggtgc caaatcgttg gttgtctact ttaacgatca atgattataa
     2101 ttctattgat caagtagtta acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag
     2161 agctttgtgg aaaccactac atttgcaacc agtatttcaa ggggtaaaaa tgtattacca
     2221 tgataataat cttagtgtgt cagaaaagtt atttaatagc ggcttatgct taccttctgg
     2281 aagtaatatg acagatagtg aacaagaatt tgttattgat gagtttaaaa aaatattcaa
     2341 gtaggtgtga tattttatga acataggaat tattggttta ggttccatgg ggaaaagaag
     2401 acttagactc attcaagaac actttaatga tattttacta tgtggtgttg atactaatga
     2461 ggcgagacga aaggaagttg aaaaggaatt tggaatacca acttataata gtctaacaga
     2521 ggcaaaaaat ttgtttgaaa taaattcagt ttttgtttgt acatccccta taacccatga
     2581 atttattata aaagaggctt taaatttaga ttgtaatatc tttacggaaa tcaatttatt
     2641 aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat tactttatct
     2701 atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag tgacgcataa
     2761 taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc atccttggga
     2821 agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag aaatattcgc
     2881 tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta ctgtaatgac
     2941 ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta ttattgagca
     3001 tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag ctaaaagaaa
     3061 tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg attctctatt
     3121 ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg aggttatgca
     3181 tgataataat tattcacata ctattattga agatgcatat ttagaggaga ttaaagaatt
     3241 tttcacgctt ttggaaaaga aaatggaacc ccaatatact tttgaaaaag ataagtatat
     3301 tattaactta attaacga
//
LOCUS       DAFGZE010000153         3304 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.157, whole genome shotgun sequence.
ACCESSION   DAFGZE010000153 DAFGZE010000000
VERSION     DAFGZE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.157"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            188..391
                     /locus_tag="LTU54_002990"
     CDS             188..391
                     /locus_tag="LTU54_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297622.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="LTU54_002991"
     CDS             602..1186
                     /locus_tag="LTU54_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297623.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1557..2882
                     /locus_tag="LTU54_002992"
     CDS             1557..2882
                     /locus_tag="LTU54_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBL8297624.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2875..3225
                     /locus_tag="LTU54_002993"
     CDS             2875..3225
                     /locus_tag="LTU54_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297625.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
BASE COUNT         1179 a          484 c          662 g          979 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca ctttcttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttatta tcaaatgttg taaggatttt attcatttgt taatattaaa
     1321 gaacggaact ccaaaaaagt ccgtactcta atttgcagtc tctcactctt ttagagtgag
     1381 agtttttatt agattttcaa attaattagt aaagtgatag aataaattga tcatacaaat
     1441 cataagaaga agcagggtga tagcatacct atgcttcttt ttttatgtat ttagctattt
     1501 cttctttaca acatcgaaca tatgtttgta taatgtggaa gaatggagtg ttatttatga
     1561 tggattattc aaaagagcca gtaaatgatt actttttaat cgatatgaaa agcttttacg
     1621 caagtgtcga atgtatagaa agaaatttag atccattaac aacagaactt gttgttatga
     1681 gtcgatctga taatactggt tcaggattga tattagcttc ttctcctgaa gcaaaaaagc
     1741 ggtatggtat tacaaatgtg agtagaccac gtgacttacc acaaccattt cctaaaacac
     1801 tacacgttgt tccaccacgt atgaacctat atatcaagcg aaatatgcag gtaaataata
     1861 ttttcagaag atatgtggct gatgaagatc tactgattta ctcgatcgat gaatcgattt
     1921 taaaagtgac aaaatcactg aatcttttta cgactgaaga aacacgaagt cagcgaagga
     1981 agaagctcgc tcaaatgatc caagaacgta ttaaagaaga gttaggtttg attgctacgg
     2041 taggcgtcgg cgataatccc cttttggcta aattagcctt agacaatgaa gcaaagcata
     2101 atgaaggatt tattgcggaa tggacgtacg aaaacgttcc tgagaaagta tggaatattc
     2161 ccgaaatgac ggacttttgg ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg
     2221 gaattttgag cattagagac cttgctaact ggaatcctta tacgattaag aatagattag
     2281 gtgtgattgg attacagctg tacttccatg ctaacggaat cgatcgaaca gatatagcaa
     2341 tcccgccaga accgacaaag gaaaaatctt atggtaattc acaagtgtta ccacgagatt
     2401 atactagaag aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa gtagccattc
     2461 gtatcaggca gcataattgt aaaacaggat gtgttcattt aaacattggt acgtcgatct
     2521 tagaaacgag accaggattt tcacaccaga tgaaaatacc tatcacagat aatacaaagg
     2581 aattgcagaa ttattgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc
     2641 acgttggaat aacctatagc aagcttgtgt acaccgattc gcttcagctg gatttattta
     2701 gtgatccaca aaagcaaatt aacgaagaaa atttagataa aattattgac aagattcggc
     2761 aaaaatatgg ttttacgtcg attgtacatg cttcttcaat gttagaaagc gctagaagca
     2821 ttactagaag cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt
     2881 gattacaatg atcgtggacg tttaaagtgg gcagggtttt atttatctga tcatactgaa
     2941 gaaattgatg ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg
     3001 actgaagaga ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatctatt
     3061 caaaaagaag aacgaaacgc tgaaggatac tatccagaag atattgttgg caagatctta
     3121 ggtagtgatg agctggggat ctacgttgga aaagacaaaa ttcactacga cgaaatacgt
     3181 catatcgaat tttaccagga taataaatgg agtcgattag aatgaacggt tctgttgcaa
     3241 agttttaaat aaagaataaa atcccttacg gtatctatga tttaagctag gattcccaat
     3301 aata
//
LOCUS       DAFGZE010000154         3240 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.158, whole genome shotgun sequence.
ACCESSION   DAFGZE010000154 DAFGZE010000000
VERSION     DAFGZE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.158"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            106..414
                     /locus_tag="LTU54_002994"
     CDS             106..414
                     /locus_tag="LTU54_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HBL8297626.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="LTU54_002995"
     CDS             441..740
                     /locus_tag="LTU54_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HBL8297627.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..1465
                     /locus_tag="LTU54_002996"
     CDS             914..1465
                     /locus_tag="LTU54_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HBL8297628.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1611..1904
                     /locus_tag="LTU54_002997"
     CDS             1611..1904
                     /locus_tag="LTU54_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297629.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1897..2193
                     /locus_tag="LTU54_002998"
     CDS             1897..2193
                     /locus_tag="LTU54_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297630.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2264..>3240
                     /locus_tag="LTU54_002999"
     CDS             2264..>3240
                     /locus_tag="LTU54_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HBL8297631.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIG"
BASE COUNT         1217 a          453 c          649 g          921 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat tggcagatag
     1441 tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat aagtagtttt
     1501 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1561 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1621 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     1681 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     1741 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     1801 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     1861 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     1921 ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc gaatatagat
     1981 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2041 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2101 gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg ttgtcatgct
     2161 ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat taattgactg
     2221 ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa aatggttgtt
     2281 attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata cgaggactaa
     2341 agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc aatcaactga
     2401 acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat
     2461 tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat ctactcaact
     2521 agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa gctacaatcc
     2581 tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg atggaaattg
     2641 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2701 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2761 gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2821 tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac agttaaaaca
     2881 aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag aagaatctac
     2941 tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt caagttggga
     3001 agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt ctcctgataa
     3061 aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3121 tgaaaatgga agctactatt caattaatat tgtaacggat gataatggaa atacaacaat
     3181 ggctggtgga aaacaattcc gtgtttatac agatggaaga atttctcaac gtattggaat
//
LOCUS       DAFGZE010000155         3211 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.159, whole genome shotgun sequence.
ACCESSION   DAFGZE010000155 DAFGZE010000000
VERSION     DAFGZE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.159"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..821
                     /locus_tag="LTU54_003000"
     CDS             <1..821
                     /locus_tag="LTU54_003000"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL8297632.1"
                     /translation="TMVDSNGKPILNKYGKPKKVVKMPIDNSVTLSIQRLRHLASLGN
                     ESNKQIVDRYFSNQPEPLTINEQMELLQTGINLHSIYMVDENENEIITAPAIARWVIN
                     KHTLEVTARINPEYLEWFSEVVNQYILFNLRDFVKLKSKYSMILFLELSTFWNTGMYY
                     KRINDLREILGVNPTRNNRDFINNVLKPAIAALKEYFPELSLTVEKNSSDSRKITHVR
                     LKFDKLTITDMQERLRSSEGASLVEYQQELENMEGEEKAVNDPTPEIDLSNVEW"
     gene            935..1303
                     /locus_tag="LTU54_003001"
     CDS             935..1303
                     /locus_tag="LTU54_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297633.1"
                     /translation="MENTNQTPNTAPTNQTSPAERKADLIRLYSRLEGMKLQHVPTAP
                     KAPDGFPKSAESLEFFECKVCGSTFQTILRESVASGCPKCEHDRLGILADHLPEPDKN
                     SCLNNRKKPVTVMAMGFLCI"
     gene            1477..1785
                     /locus_tag="LTU54_003002"
     CDS             1477..1785
                     /locus_tag="LTU54_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297634.1"
                     /translation="MKEMKMKQVIYPVIIGVLAIALIILGVNCYNLSKENNQLETKNN
                     KQEIQIAKQAYVISDYKTYFRENYVDTVKYRSHFTDKQILNVYREALRDGKISDEGAN
                     "
     gene            2048..2368
                     /locus_tag="LTU54_003003"
     CDS             2048..2368
                     /locus_tag="LTU54_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297635.1"
                     /translation="MERFLTKNRKGGGKLEKKEDLLNAFKNLDEKDQLKALLRLHDIT
                     YSDIAERLTVKKGEREGQKGVSLVTVQKAFQSKTNLESDRWKEIVKVATKLIEERQND
                     QNKA"
     gene            2499..2708
                     /locus_tag="LTU54_003004"
     CDS             2499..2708
                     /locus_tag="LTU54_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297636.1"
                     /translation="MIRKLNNIKLSLAILTLFFGIYNNSRMALFFLTLLIFMNLIIHN
                     FKELENTAPAPSQGNQESEGMPHAW"
     gene            2698..2970
                     /locus_tag="LTU54_003005"
     CDS             2698..2970
                     /locus_tag="LTU54_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297637.1"
                     /translation="MLGSFKKDKEVKTRGALTPSQQNKLGTKATFHPVRDQANQKELI
                     GVCVGFTSEKFFPIIECKIHGITTREVARKVVKWHTSQTKTNHKKR"
     gene            3018..>3211
                     /locus_tag="LTU54_003006"
     CDS             3018..>3211
                     /locus_tag="LTU54_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297638.1"
                     /translation="MNNKNTKNTNTNHEKRSFFDKLFKKKKNGKVSILKMIGWIFLAF
                     FIVAVNSSGGDKKEAASKDTT"
BASE COUNT         1212 a          577 c          647 g          775 t
ORIGIN      
        1 taactatggt agatagtaac ggcaagccta tcttaaacaa atatggaaag ccgaaaaagg
       61 tagtaaaaat gccgatagat aactcggtta ctttgagtat tcagcgccta cggcacctag
      121 caagcttagg aaacgaaagc aacaaacaaa tagttgatcg ttacttttca aaccagccag
      181 aacctctgac aattaacgag caaatggaat tactccaaac ggggataaat ttacactcta
      241 tttatatggt agatgaaaat gaaaatgaga tcataaccgc gcccgctata gcaaggtggg
      301 taataaataa gcatacgcta gaagtaacag cgcggatcaa tccagaatat ttagagtggt
      361 tttcagaagt ggttaaccaa tacattttat ttaatctaag agactttgtg aagttaaaaa
      421 gtaagtactc catgatcctt ttcttagagc tttctacctt ttggaataca ggaatgtatt
      481 ataaaaggat taatgattta agagaaattt taggagtcaa tccaacgaga aataacagag
      541 attttataaa caacgttcta aagcctgcta tcgctgctct caaagagtac tttccagagc
      601 taagcctaac agtggaaaag aattctagcg actccagaaa gatcactcat gtaagattga
      661 agtttgataa gctaacaata accgatatgc aagaacgcct aagaagcagc gagggagctt
      721 ctttagtaga gtaccaacaa gagttggaaa atatggaagg agaggaaaaa gctgtcaacg
      781 atccaactcc agagatcgac ctttccaacg tggaatggta acacctgtca gcaatctaat
      841 ccaataaacc aaagtacttg taaggcgcga gggctccttt tagagccttc tagtcctata
      901 ccaaaccaag caaaataaag gagctataac caacatggaa aacactaacc aaacaccaaa
      961 tacagcacca actaaccaaa ctagcccagc cgaacgcaaa gccgatctta ttagattgta
     1021 ctcacgcctt gaaggtatga aactccaaca cgttccgacc gctccaaagg cgccagacgg
     1081 cttcccaaaa tcggcagaga gccttgagtt tttcgagtgt aaggtgtgcg gatcgacttt
     1141 tcaaacgatt ttaagggaat ccgttgcgag tggctgcccc aaatgtgaac atgatcgctt
     1201 aggaatttta gccgatcatt taccagaacc agacaaaaac agttgtttaa ataatcgaaa
     1261 aaaacctgta accgttatgg ctatggggtt tttgtgtatt tgagataatt tccaagggtt
     1321 atttatggat ttattttaaa aaatctatta aattatacta ccactggtag taatataaat
     1381 atatcgaaag ggtacgaggg ttaaagcgta atggcggaat gagtaccgtt ctcttttgaa
     1441 aataccaaac caaccaatta aaggagagaa agtaatatga aagaaatgaa aatgaagcaa
     1501 gtaatttatc ccgtgattat tggagtttta gctatcgctc ttattatcct tggtgtaaat
     1561 tgctataact taagcaagga aaataatcaa cttgagacca aaaacaacaa gcaagaaatc
     1621 caaatagcca agcaagctta cgttatcagc gattacaaga cttattttcg tgaaaactat
     1681 gtagacaccg tgaaatatcg ctcccacttc actgataaac aaattttaaa tgtttatcga
     1741 gaagctctta gagatggaaa aatcagcgat gagggagcta actagtggaa gagctcattt
     1801 tgctggctat gttattacag gaaaagaagc gtgaacgaga agcctaacag ctggaaagaa
     1861 tccgaatagg atatgaggaa gcagatcggg aaagagatca gaaaatagag gagctggaga
     1921 agcgtatcca agaactttcc aaaaactatg ataataacca agattgggaa caagagtggc
     1981 ggaaaatcgt tggtataaag gaaaatgaat acttagtgag aaagccgcca gaagaagagg
     2041 aagacccttg gagcgatttc taactaaaaa tcggaaggga ggtggtaagt tggagaaaaa
     2101 ggaagattta ttgaacgctt ttaagaacct agacgagaaa gatcaactta aagcgttatt
     2161 aaggcttcat gatataacat acagcgatat tgctgaaaga ttaaccgtta aaaaaggcga
     2221 gagggaagga caaaaaggtg ttagcctcgt gactgtacaa aaagctttcc aatcaaagac
     2281 caacttggag agtgatcgct ggaaagagat tgtaaaagtc gctaccaagc taattgaaga
     2341 gcgacaaaac gaccagaaca aagcataaaa aaagcccgtc catttaccgc tgaccaaagc
     2401 aataaaatga acgagccaca ccatttactt ttaggggtaa ccacacgccc taaaagctac
     2461 caaaccattt accaaataac cactaaggag atttagaaat gattagaaaa cttaataata
     2521 tcaagctttc tttggcaatt ttaacactat tttttggaat ttacaataat tccagaatgg
     2581 ctttgttttt tcttacgtta ttgatattca tgaacttaat tatccataat ttcaaggagt
     2641 tagaaaatac agctccagcg ccctctcaag gaaaccaaga aagtgagggc atgccacatg
     2701 cttggtagtt ttaaaaagga taaggaagta aaaacacgag gagcccttac accaagccag
     2761 caaaataagc ttggtacaaa agccactttc caccccgtcc gagatcaagc caaccaaaaa
     2821 gaactaattg gcgtttgtgt agggtttact tcagagaaat tttttccaat aatagagtgc
     2881 aaaatacacg ggatcacaac tagagaagtt gctagaaaag ttgtgaaatg gcacaccagc
     2941 caaaccaaaa ccaaccataa gaaacgttaa aaacgttcct aagagcgttt gaaaattaaa
     3001 ttaagggaga tttaagaatg aacaacaaaa acaccaaaaa taccaatact aaccacgaga
     3061 agagaagctt ctttgataag cttttcaaaa agaagaagaa tggaaaagta tctattttga
     3121 aaatgatcgg ctggatattt ttagcctttt tcatagttgc tgtgaacagt agcggcggtg
     3181 ataagaaaga ggctgcttca aaagatacca c
//
LOCUS       DAFGZE010000156         3199 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.160, whole genome shotgun sequence.
ACCESSION   DAFGZE010000156 DAFGZE010000000
VERSION     DAFGZE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.160"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..635
                     /locus_tag="LTU54_003007"
     CDS             <1..635
                     /locus_tag="LTU54_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBL8297639.1"
                     /translation="MINTWNNIKDSFWNIVTGIVNSAENAWTNLKNGVSNAINRVKEI
                     FNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIKGIAGNIKNWIKGALDIHSPSRW
                     MRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPSVQPVVNGISHLPITQTKQNISA
                     SETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQMKRDAGAVGGTLYAT"
     gene            625..1365
                     /locus_tag="LTU54_003008"
     CDS             625..1365
                     /locus_tag="LTU54_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8297640.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYRNVTRKIECYYKAPSIDVVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1362..>3199
                     /locus_tag="LTU54_003009"
     CDS             1362..>3199
                     /locus_tag="LTU54_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBL8297641.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLAEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRLIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     SLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFTSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSAGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1195 a          396 c          624 g          984 t
ORIGIN      
        1 ccatgattaa tacatggaat aacattaagg actctttctg gaatattgtt actggaattg
       61 ttaattctgc tgaaaatgca tggacaaatc taaaaaatgg tgtttcaaat gctattaatc
      121 gggtaaaaga aatctttaat tcgttaaggg aagtcaattt atttgaaatt ggcaagaaca
      181 tcattgatgg acttataaat ggtgtaaaag aaaagtggaa tgctttgaaa gatactatca
      241 aaggtattgc aggtaatatc aaaaattgga ttaaaggagc tttagatatt cattctccat
      301 caagatggat gcgtgatatg gttggtaaaa atattgttca aggtattatc gttggtattg
      361 ataaagagca aagtaagtta gatcaaacca tgacaaattt agtaaaaaca ccgtctgttc
      421 aacctgtagt aaatggaata agtcacctac caattacaca aacaaagcaa aatatatctg
      481 ctagtgaaac aaaagaaatt catttacatt taaatgttta tggagattta cctgattcaa
      541 tggttcggaa gattaccaca aaaattaaaa cagaattgac aagacaaatg aaacgagatg
      601 ctggtgcagt aggaggaaca ttatatgcaa cttaagagag gacaattttt tattaatcaa
      661 cattattctt ctgaatttaa tgtgtatatt caaaacaggc ctgcttctgt ttcagctagt
      721 cgagtaattg aattgagaga gcgagaagga aatgattcta tcattattga taaaggctat
      781 tacagaaatg tgactagaaa gattgagtgt tactataaag caccttcaat tgatgtggtg
      841 caggagtggg aagatcgaat cactgaatgg ttagatatgt cgtcttatag tgattttatt
      901 ctttactatg atgaacaata tatttatcaa gctgtagtaa tagaaggacc tgaatttaaa
      961 ggaacaagaa aaacaggaaa tattgtccct tttgaattta cagtgagcat tcgaccattt
     1021 aaggaaaatt acaatggtag atttactatt cgacaaacag aaacttttga aatttataac
     1081 ccagagaagt attcttcaaa accgcttatt aaattgagtg gttctggaga tgcttctttt
     1141 tatattaata aggacaaata tgatttgaaa tcgttggaca gagaattatt tatagattct
     1201 aaattagaag aggcttaccg aaaattagat ggtaatttag aacatcagga tcaagtcact
     1261 ttatttttag attttccatt tttatatcct ggaaaaaatg aaattaaatg gacgaaaaat
     1321 attcattcat ttgaaataat gcctaggtgg tggagaaaag tatgaaacca agaatatata
     1381 gtcctactga aacagatttc tcaacgaatg gcttaggaat tttaaaggat tgtacaagat
     1441 gtgaaatata tgaagtagct aatggaaaat atgaattaga gttggattat cctttaggaa
     1501 ctagatttga tgaatatttt gaaaatgact atcaaataaa agcaaagcca aacgatcaag
     1561 aagagtatca tatctttttt attgatgata aagatataga tactttttta aatacagtaa
     1621 ctatttatgc tcagagtcgt acaaatcgac ttggaagacg ggtagtcact cttgcggaag
     1681 tagactctaa aactggtcaa gaagcgatat caattattga aaccaaaatg gataaaaaat
     1741 ctgacatacg actttattct gatattacgg ccgtttctag cacaatcttt gaagcgagaa
     1801 atgttttaaa ttgtattgct ggtgaacaag gatcattgct tcaatattgg ggtggagaaa
     1861 ttaaacgtga accatttaag ctttctttgt taaagcgaag aggtcgcaat aatgttggaa
     1921 cgattcggta tggaaaagat atgtctggtt taaaggtcaa attagattgg acaggtataa
     1981 aaacaagact tattccatat gctgatcctc aaagtgaggt aggtacgact aatcgaattt
     2041 atggttcgcc agtagatagt gcctatatta ataactatcc tgatgtgtat acagagcatg
     2101 ttcagtttac agaagaacaa ggagtaaaag acattaagag cttaaataaa atagctaaaa
     2161 attactttaa aacgatcaat cctggctgtg ataagcctaa agtttctatt acggttgaat
     2221 ttgataagtt gactgatagt gaagaagcga aagaatttgc gaagattaga aattatggtc
     2281 tgtttgatac atttaaaata tatcataaaa aatataatat ttatcttgaa tcaaaggtta
     2341 gtggactaca atacgattct ttatctgaaa aaactttgaa attagaagca ggagacattc
     2401 aagttgcttt ttatcagcaa caagctgtaa ctattcagga taaattaaaa gattatgcaa
     2461 cgaataatta tatgagtgat tttaatgatt atgtttcttc aatgattaca ggtcaaggca
     2521 gtgcaggagg atatgtaagt ttatggccaa aagaaaaacc ttccaacatc tttattatgg
     2581 ataatccaga tttagaaaag gcaagagaag tactaagaat gaataaaaat gggattgctt
     2641 tttctaagaa aggatggcgg gggcctttta cttcagcttg gacattagat agtatattta
     2701 atgccaattt tatacaaact ggattaatca aagcagatat ctttcaaaat tcttttaaca
     2761 agactggaga tgtattgaaa ttagttaatg gactactcca aatttggaac aacaaaaaga
     2821 aaataatgga gttaactaaa aaaggaatgg aattttggaa ttctaagggt tcaattggaa
     2881 cgattggaac aactgattct gctggtaatc cttttcctgg ggcttctact ccaaccccta
     2941 ttgaagataa ttctttagtt attcgtacaa atggagacgg caaatatatt ttgatttctc
     3001 ctaaagtagg aaaaggatta gttttattag gaaatggtaa agcaatttat tttggagact
     3061 tagatgtaca aggaaaactt acagttaaag gaaaagaaat aattcctgga caaggtggtg
     3121 gtccatcggg aggtggagaa tctactggtg ggtatcctag tgaattaaaa acagatgcag
     3181 aaaagagagc ttggagaat
//
LOCUS       DAFGZE010000157         3041 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.161, whole genome shotgun sequence.
ACCESSION   DAFGZE010000157 DAFGZE010000000
VERSION     DAFGZE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.161"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            138..752
                     /locus_tag="LTU54_003010"
     CDS             138..752
                     /locus_tag="LTU54_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297642.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(776..1126)
                     /locus_tag="LTU54_003011"
     CDS             complement(776..1126)
                     /locus_tag="LTU54_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBL8297643.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1120..1383)
                     /locus_tag="LTU54_003012"
     CDS             complement(1120..1383)
                     /locus_tag="LTU54_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBL8297644.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            complement(1724..1903)
                     /locus_tag="LTU54_003013"
     CDS             complement(1724..1903)
                     /locus_tag="LTU54_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297645.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            complement(2284..2991)
                     /locus_tag="LTU54_003014"
     CDS             complement(2284..2991)
                     /locus_tag="LTU54_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297646.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
BASE COUNT          925 a          589 c          469 g         1058 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacttg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gcaaaagact acaatgttac gagacaaacc gtatatagaa
      721 taaaaaaaga cagcatggtc aatcacgaat agttgatcat gctgctgttt ctattttatt
      781 tgcttacttt ttgaaaaact gttctaactt tcatcaataa ctcctcaact aaactatcat
      841 gcgctttttc caaaaaaata cactctctgg cttcataatc aattgtcgtt agttgatcta
      901 ataatacttt tcctgttgtc ttcatttcat attctgagtt tatatttaca tatagcggat
      961 agtttctagt tgtactagaa attggagcaa ctgtaacaac gttactataa tccgcaataa
     1021 tatcatgact taaaacaata taaggtcttt tacctgtctg ttcgtgtcca gatcgaggtg
     1081 ctgtattcaa taatagaata tcgccttgtc taggttttct caccattttt cattacctac
     1141 tgcttcacct aaattttgaa tcgtcacatg atttctttca tcagtgtaat tttcaaataa
     1201 ctgttcaatt gttaattcat tttctatatt agccgcttcg ataattagcc gcttctgacc
     1261 gtttacttct tcttgtatta tcttcacttt gtcttctgta tgaattccaa gatattccat
     1321 aatatacttt ggcaatctaa ggccattact atttccccat ttttttatag gtaacatttc
     1381 catagtagac acctctttct gtatatacat agtataacat acataagaaa aaacgtatat
     1441 acttaattat ccttttctat taaattagat taacattcca tgaatccata aactatgtta
     1501 acatttatat gaaaatagtc gaaaggaaat cataatatgt ttaagaaaac aaaatcttta
     1561 gaatcaggta agaaaaaaat tgatctaaaa ataatctctt ctggatactt taaagatttc
     1621 atagttattg atacaaaatg gaataaaaag gacatagaaa atattctaaa tagtaggaac
     1681 aattaagtgc tatcactttt gtttcttcaa atgaaaagtg acgtcacttt tttagtgctg
     1741 gtatatcaaa tcctgctttt tcttgcgcga tcaatagatc taacgcaata cacataatct
     1801 tagattcact ttttccgtac ttctgcttta gttgctgcaa ttctttttgt ctcttttcat
     1861 ctaatgtcac gggtaaccgt ttatttccta ttgcaattgg cattagtact ctctcctttc
     1921 atcgaaataa ccctatgaaa aatatggctg caatcatctt gaatagtaga ctcacaaggg
     1981 ctaatacaaa tcgccaaaag atcaaacaaa ataccagtat tcctaagaat actacgaagt
     2041 ttaaatgtac agctgcagca tgacgcataa gtatctcgcc aacaaacagt acgaacaata
     2101 gaggaatgat tttattcata tggatctcct tctttctctt aggttatcgg gctttgcccg
     2161 cttatttcgc accacctaag cggcgggggc ggttggagcg attggtgcga aataagaacc
     2221 attcccttaa tttcaaggtt ttttgttctt atttcacaca tcctcacttt tcggtataaa
     2281 tggttattta ccttttttcc tttcaaatcg ttgatatggc attcgtttac ggtcaagttc
     2341 attttggatt tcttctgtat cgtcgtaagt gtaggtgtct aaaaaatcag ttaaagtcgt
     2401 gtaccagtcg tcgcctgggt tatctacttc ttctacatat tccctcaaaa atgcttcggg
     2461 tacagcgagt tcataatcca ttcccattaa cacgatagat tccgtcccac acgtcaaaaa
     2521 cctagtaaat acaaggtaat aatcgtctac cgtcgcattt ttgctgttta gctcttccat
     2581 ttcttcttcc attaattcca tgctctcgaa aattgaataa tattctgatt ctgagtaata
     2641 gtgtttccta caaatcgtgt agctgacaaa gttttcgacc agctcactcc tttctaggta
     2701 cacattcatg atgtattttt ctgtttctag tcgtagaagc tctgttcctt tttttaccgt
     2761 ttcaggacga cgtttataca cgtctaaatg attatggctg actgtgacct tcgtttgact
     2821 tgtttcgctt tccatataca actgttcttc cactctttga ataaagtact tcatttcttc
     2881 catacaacta aagtcttttt gaatccgtct gattacttca ttcaagtgtt ctttgcttgt
     2941 taaaaattgc gaaacatctt ctgttgttag ctgataaacc accttattca ttatctttct
     3001 cctccgattc aatcgtataa aatgcaccat ttttttcgat a
//
LOCUS       DAFGZE010000158         2867 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.163, whole genome shotgun sequence.
ACCESSION   DAFGZE010000158 DAFGZE010000000
VERSION     DAFGZE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.163"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            30..686
                     /locus_tag="LTU54_003015"
     CDS             30..686
                     /locus_tag="LTU54_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBL8297647.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            686..2062
                     /locus_tag="LTU54_003016"
     CDS             686..2062
                     /locus_tag="LTU54_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8297648.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2348..>2867)
                     /locus_tag="LTU54_003017"
     CDS             complement(2348..>2867)
                     /locus_tag="LTU54_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297649.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSLEQLLSTVCDVGDISFSIRNQLRFKRSLPVSGNINVYFTKARFKRLIAITVSSIV
                     MFLWLFRMGVIP"
BASE COUNT         1088 a          384 c          509 g          886 t
ORIGIN      
        1 tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag aatgtagcat
       61 atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa tttgaacttg
      121 ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt ctttccttac
      181 ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa gatatacaaa
      241 ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa aactataatt
      301 taatagatta tttatcacca attgaaaata ttagattagt aaataaatca gcagatgaaa
      361 gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt atgaaattat
      421 ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat gctccaataa
      481 tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa ataattaata
      541 tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca catagtaagg
      601 aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg aaaaaagtaa
      661 ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg taactagaaa
      721 aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt taagtctcat
      781 tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg ctaatataac
      841 aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta gaggtggggg
      901 aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag actcctatgt
      961 aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa ctcaagagac
     1021 tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta tgttaactgg
     1081 ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat tagtagaagg
     1141 aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt tagctgaaaa
     1201 aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg atgctgataa
     1261 tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg atgggcataa
     1321 tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca ctgatattca
     1381 tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag atgcaacatt
     1441 ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga aattagatat
     1501 aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat tacaacaatc
     1561 tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa tatttgcagg
     1621 agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag aaatagcagt
     1681 attactatca ttaggtatat caaaattaga aatttttggt caattcctaa ttgagatggt
     1741 atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata cagctgacaa
     1801 gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa tagctaaaca
     1861 atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca agacattatc
     1921 aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat ttatgagttt
     1981 agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga atccaaaaga
     2041 attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa tgagagtgta
     2101 atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg gtttttattt
     2161 ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa atcctgatgc
     2221 tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag aggatatgca
     2281 agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag gagaattgaa
     2341 taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca atcgacgaaa
     2401 cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg atgttcccag
     2461 agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag atgtctccga
     2521 catcgcagac tgtagacaaa agctgttcca aactcgtaac tcgagcttgg aacagctttt
     2581 tatctacttc gtttcgaatt tttgaacaaa aaaataaagg ctccttgcct ttccatagcc
     2641 aggtggctaa tttctttaaa ttcattgcag caaacactag catcgtatgc gtcgcgactt
     2701 tttccaagcc tcgatacttg gtccaacgca tcccatgctt ttcttttgca tccgcaaata
     2761 ttcgttcaat cgtttgtttc cgttttttgt aaattgattt attcagatct gttaggcgaa
     2821 gatgttccac ttcatccatc agatctcccc aaacatgtct cgtgatg
//
LOCUS       DAFGZE010000159         2840 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.164, whole genome shotgun sequence.
ACCESSION   DAFGZE010000159 DAFGZE010000000
VERSION     DAFGZE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.164"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            185..481
                     /locus_tag="LTU54_003018"
     CDS             185..481
                     /locus_tag="LTU54_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297650.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            483..905
                     /locus_tag="LTU54_003019"
     CDS             483..905
                     /locus_tag="LTU54_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297651.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            924..2516
                     /locus_tag="LTU54_003020"
     CDS             924..2516
                     /locus_tag="LTU54_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297652.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            2537..>2840
                     /locus_tag="LTU54_003021"
     CDS             2537..>2840
                     /locus_tag="LTU54_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297653.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1145 a          414 c          558 g          723 t
ORIGIN      
        1 ccccactttt aaaaaaagtg gttggatagg ctgtgcctat cacgtttctg aaaaaaataa
       61 atgaccccaa aataccaata tataggatag tacaaggata gcaaaggata gtaaaaggat
      121 agaggtagga tagcgaaaag gataggatcc ttttcactat cctatattat attggaggat
      181 atttatgaat gttaaaatta gagatttaga tccaaaattc atttctgaaa ttgatcgaag
      241 atgcgttgaa ctaagtaatc gtacaggaaa taaatggagt cgtaatgatt atttaaagct
      301 actcgttgaa aatgattttg atcgtccttt gatggaatac aaacaagaaa agtttgacca
      361 gttattagaa aagttttcag aaattcaaag ttataacaca aagatattag aagaatatgt
      421 tagccaaaat aatcgtatta tagaaatctt gattgagcaa aatagaggaa gtgaactttg
      481 atatggcaaa ggttatagac aaacacattt actttcacca aacacatatc gatgtattgg
      541 atgaaactat ttctactcat gcagaggtta aaaactatag tgaagctgtt cgctttttat
      601 gtatgaacta tgatccagaa gtggagaaaa aagtaaattc caatcaagca aagttgaatg
      661 gaatgagtaa agaaatttct attttaaccg aaatggtttc agaatttgcg gatcttcatt
      721 tgaaagatac aggaatatta attggtaaaa attcaactgt ttatcaagaa gcaaaagaac
      781 gagtggaagg aaaaattaaa tccaatcaaa caaaaaaata ttcccctcag ttgaagaaac
      841 ctgtgaatga atctgttcat gccagagtag atccatttga tccaacgaag attctaaaaa
      901 gataaaaatg aaggaggata ataatgactc aatcgccttc agttattctc acttgtcaat
      961 ttactttgcc gaataaaaag tcattttcta cttatattga ctatattaca agggaacagg
     1021 ctcttgaaga aaaggaacga cgtacgcccg aagaagaaca ggaacttcaa cgaatcaaga
     1081 atgctttaga ggagttttat attcctgaag gagaaacttt ttcggagagt aagacgggta
     1141 aagaattcag tgaaacgtcg aatgaagctc aatcgttgat ggaacatgga atcaattttg
     1201 acaagttaga ggaacaagat tttaccaatt atctttctta tatgacacgt cactatgcgt
     1261 tagaacaaaa gaaagaactg acgcaaagtg aacaaaaaga ataccaacgc ttgaaccaag
     1321 caatgaaaaa atatcaaaca gaaccccata aaaaagcttc aggaaatctt cctggagttt
     1381 tttctatagc ttcagacgaa gtgaaaggaa gtgatctgaa ggagattaag caaatcttcc
     1441 aaagaggaca acaaaaaggc tcaatcattt atcaagatgt tgtttctcat gataattcgt
     1501 atcttgaaaa aataggattg tacgatccca aaacagatac tttagatgaa gaagggttaa
     1561 aagcagctgg aagaaaaatg atggagacat tattcgagaa agaacaattg aatgaaacag
     1621 gttactggat ggcaacgatt caccgtaaca ctaaacatat ccatattcat tttgcagttg
     1681 ttgaaaaaga aaatacgcgt aaaccttaca cggaggttga aaatggtgtg agttatatcg
     1741 taccgaaagg taagagaaga caagcaacat tagacgcaat gaagaccaat tatgtacatg
     1801 agttagaacg tttttcttat gaaaaggatc gaatgattgg tatggagaaa cgaaatttat
     1861 tgacgcggaa aagtgatttg cgtaacacct taacaaaagt ggtaaaagag ccagcgcgtt
     1921 acgatcgaca cgcgatgaac ttattaaatg aagtatataa aaccctacct gacaaacgtt
     1981 cggaatggaa ttacggggat gaaaaacgaa ccaaactttc accatacaca agagagaaac
     2041 tcgatgagtt aaccaaatat gttttaacaa aagaacctga atatcaagaa tatatacaga
     2101 tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa
     2161 aggatgcaga gaaaaatgct ctctttgatt taaaaaagcg cacaggaaat gccatcctaa
     2221 cagagctgaa gagacaggat agtcaaataa aaccattaat agaaagtgtt gatcagatgc
     2281 aatctattta caatgaacga tcctttcgaa cgcttaaaca attgccaacg aatgaataca
     2341 aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc
     2401 agcggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa
     2461 gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaaagac
     2521 agtaggagga atagcaatga aacaatattt agatcaatgg aaagttatag aaggatcatt
     2581 aagagaagaa cgaatcgaac aacttcctga ttgtttagaa aaagagcatt tgttccagat
     2641 aagagaaatg ttgagaaacg aacagtttga tccaaatcaa tttttagtcg ttgagtaccc
     2701 agctactggt gtgtattgtt gtaatcatgt gaatggtgaa aaatatttta tcattcaaga
     2761 atatgagggc aagttagcac cttattatac tacttgggaa atgaatgaag agggaatcaa
     2821 taactttcca tgtaaaagca
//
LOCUS       DAFGZE010000160         2832 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.165, whole genome shotgun sequence.
ACCESSION   DAFGZE010000160 DAFGZE010000000
VERSION     DAFGZE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.165"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            47..1216
                     /locus_tag="LTU54_003022"
     CDS             47..1216
                     /locus_tag="LTU54_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HBL8297654.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            1473..2768
                     /locus_tag="LTU54_003023"
     CDS             1473..2768
                     /locus_tag="LTU54_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL8297655.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          951 a          538 c          496 g          847 t
ORIGIN      
        1 agctttattc tttctgtaaa aaagaaagga gactaaataa actattatga aaaaaggaaa
       61 acatgctaaa aaaagaaaat attcattttt aatgatcgtt gtcgtgttgt ttagccttag
      121 tgcgttctct acaggaacta ttcttggatg gcaattttac aataatgaac actctcagat
      181 agttgcccat gctcaatcag ctcaaaaaaa gttgatactg actaccaatg aacaacagct
      241 cgttgaaaaa atcgatcaac tattaagaaa aaatgattat attggctcaa tttacgtaaa
      301 gaaaaataat cgcatcatca tggaaaaagg ctatggctat gcgaataaac tcacgcaaca
      361 accaaatgat ccttctcttt attatcagat tggttcggtt caaaaagcat tgactactct
      421 tttgatttta aaacaagtcg agcgtgggca cctttcttta gatactaagc tggctcaatt
      481 ctatccacaa attgctggca gccagcaaat tactatccgt gacttgctgt atatgcgctc
      541 aggcttgcac cgaactgctt ctccaaccat accgatgact gatgaagaag tcgttcaatt
      601 tgcgattcag cacttaaaat ttacagatta tcagacctct cattatgatt cattgaactt
      661 taccatattg actggaattt tgatcaaatt aacaaatcaa ccgtatgaaa aattattgac
      721 cgatgatatc atcgaacctt taggattaca gcatacaaaa ttttatgacc aagtcaaaaa
      781 ttcatccaat catgcactat cctatcaaat gtctaaagag gatgattact ttaaaccact
      841 gacagaatct gaaacagaga tccataacga attagggact ggtaatatca gtatgtccgt
      901 ctatgattta aatacgctgt ttacacaagt tttatctgga aaactagttt ccaaagactt
      961 actcttctca ttatggcgtg aaaatgcgaa tggacatcca tatagtggtg gggtatatag
     1021 cggagataat tatatcctgt cgcaaggaaa tatcgactct tttgatacat ctgctatatt
     1081 tagcaaagat tcacaggatg ctatcatcat ggaaagcaat ataaaagctg acaaaactat
     1141 caaactgcct actgctgatc taagaaacca aatctatgac ctgatggaag gaactactaa
     1201 gttggcaaat agctgatatg atgaaaagat agcaaatact acttctttga atagcatttg
     1261 cttaaaaaaa gtactcgact aaacgtagga aagagtgtgg gacaaatcta aaaatcaatt
     1321 tttgctacag actttaattc caaataaaca ggctctatac tttttgatgt tggtgaactt
     1381 caccaacatc ttttttgtat aaaaaaatat agaaaagaca ccctgaaatc ctttagaatt
     1441 aatttggcta ccaattcata ggaggattca agatgtctca tactcagctt atcaaagata
     1501 ccctaaatat tctagaccta aatatctatt tcgaggaaaa ttgtttaact aaagaaaaat
     1561 acaaagggca aatttgcttg atttacaaag gaactcttct gtacaagcca gaagagtgtt
     1621 cccattgtct ttgtgtcgtc ccctctagga tcattcgttg ggggacgaca accgttcggt
     1681 tgttactgaa tgatgtgtct gagtatcgca cttatctgga gctgaagaaa caacgattta
     1741 aatgtaagtc ttgccaaaga acattcgtag cggatacctc tgttgctgaa aaacactgtt
     1801 tcattagtca aaaagttcgt tggtctgtca ttgctcgctt aaaagagaat acttccatga
     1861 cggaaatcgc tcgtcagaaa aatatttcca cttcttcggt ctatcgtgtc atgaagcgat
     1921 tctaccgccc attgaatcct ttcaaacaga ctttgccaaa ggtcttatgc tttgatgaat
     1981 tcaaatcggt tcgaagtgtt gccagtgcaa tgagttttat catgatggac ggacaaagcc
     2041 atgcattatt ggatattgtc gaaaatcgac gcttgcctta tttagaacgc tatttttctc
     2101 ggttttcatt agcgactcgt gaagccgttc aattgattgt tattgatatg tatgcgcctt
     2161 atgtttcgtt ggtgaaaaag cttttcccga atgctcagtt gatcatcgat cgtttccaca
     2221 tcgtccaaca tattggcaga actttcttga atcatcgagt gaaagaaacc acggtacgac
     2281 ttaaaggaaa ttcccactct caacgagggt tagggaaaaa attgaagcgc tattggaaac
     2341 tcctacaaaa agaagaagca aagttaaact atgaaaaacg ggtttggcgt gccagtttta
     2401 aagattattt gactgaaagt gagattgccg atcgactgtt agctgcttgt cccaacttac
     2461 gccaaggcta tcagttatac caagacattc tttatgcggt aaagaaaaga gatcagtcac
     2521 tatttgagga ttgtttaact agagacatta gcggtctgcc agaaacctat acaaccactt
     2581 tacgaacgtt taaaaagttt ctgccacaga tccaaaatgc gcttcactac agctattcaa
     2641 acggaccatt ggaatgctta aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct
     2701 ttcgcaactt ttacaatttc aaactacgaa ttatgattcg tcatggcaaa acatttttaa
     2761 ccaaataaac agaacagaaa gaggttttct tctttctgtt ctgtccaaat tattctaaga
     2821 tttctcttta cc
//
LOCUS       DAFGZE010000161         2826 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.166, whole genome shotgun sequence.
ACCESSION   DAFGZE010000161 DAFGZE010000000
VERSION     DAFGZE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.166"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..1388
                     /locus_tag="LTU54_003024"
     CDS             <1..1388
                     /locus_tag="LTU54_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HBL8297656.1"
                     /translation="EVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGV
                     IEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPE
                     GTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQ
                     KVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEV
                     EVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVS
                     TAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEV
                     TVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKD
                     SGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
     gene            1522..>2826
                     /locus_tag="LTU54_003025"
     CDS             1522..>2826
                     /locus_tag="LTU54_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBL8297657.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNF"
BASE COUNT         1104 a          387 c          553 g          782 t
ORIGIN      
        1 tagaagttgg gggcacagta gatctaacag ataatgttac taacttgcca gcgttaccag
       61 aaggaacaac agtaacagat gttactcccg gcggtacaat tgataccaac acaccaggta
      121 attacgaagg tgtcattgaa gtaacgtatc cagacggtac gaaagatacg gtgaaagttc
      181 cagtagaagt gacagacaat cgttctgacg ccgataaata tgaaccaaca gtagaaggcg
      241 aaaaagtaga aattggaggc acagtagatt taacagataa tgttactaac ttgccagcgt
      301 taccagaagg aacaacagta acagatgtta ctcccggcgg tacaattgat accaacacac
      361 caggcaatta tgaaggtgtc attgaagtaa cgtatccaga cggtacgaaa gatacggtga
      421 aagttccagt agaagtgaca gacaatcgtt ctaatgcaga taaatacaat cctgaaggac
      481 aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt aagaacaaag
      541 aagatttacc aaaaggaact acttatgctt ggaaagagaa agtagatgtt agcacagctg
      601 gaaataaaaa aggtactatt gtagtgacat atccagatgg atcatctgat gaagttgaag
      661 tagacgttac agtgacagat actcgttccg atgcagataa atataatcct gaaggacaaa
      721 aagtgactac agacttaaat aaagagccag atgcatctga gggaattaag aacaaagaag
      781 atctaccaaa aggaactact tatacttgga aagaaaaagt agatgttagt acagctggaa
      841 acaaaaaagg tactgtagta gtaacatatc cagatggatc taaagaagaa gtagaagtta
      901 ctatttctgt agtagataaa aaagcaccga ataaacctca agttgatcct attacagagg
      961 gtgaccaaat tgttactggt aaaactgaac caaatgcaga ggtgacagtt acattaccag
     1021 acggaagtca acaccatggg acagctgata aaaatggtaa ttttacagtt aaagttccta
     1081 aattagaggc agggacaaaa gtgatagtaa ctgcaactga tgaatctggt aatactagtg
     1141 aacctactaa tgtagttgtt tcttccaaca aaaaagacag tggaaaaaac ggtagtaaag
     1201 gtagtaagac tgacaatcaa ggtagcaaca gcaatcaaga caaaaataga ggcaaaagcc
     1261 agtcctcaaa agtttttcct aagacgggag agagtgacag taatatcttt actattagcg
     1321 gtggtttaat actgttagga acgttaggat tattagggta taaaaatcga aaaaaagaga
     1381 atgaatagaa aatcaatttc ttaagtcttc tctgagaaga ggaagaatct ttttcttcct
     1441 ctttttgctt ttttatgatg ggaaataagt gttagttaaa gatgaatata ctttttatag
     1501 aaaggaaaag aatgtaatta agtggaaaaa atagtaatta ttggttcaaa ccattcaggg
     1561 ctagccgcag ctcgatttct aaacttaagc gattcaaatt atgaagttat tctaatcgat
     1621 aaaaaatcag aaattagtta cttggaatgt gggacatcgt tatttataaa aggagaagta
     1681 agtaatttag aagatctttt ttatcaaaat agaatgacac taaattctga gataaaaaag
     1741 ttatttctag agacagaagt tgacaaaatt gatattcaaa ataaaaagat tagttgtgta
     1801 aaaaaagatg gtacaagatt ctctcaagaa tatgacaaag ttatattagc tacaggttca
     1861 aagcaaatta tgctagatat accggggaat aatttagcta atattttttc gataaagaat
     1921 tttccaaatg caaaagagat ttttaaagct ttggatgata gtactattaa aaaagtagga
     1981 attattggag caggatatat tggagttgaa attgctgaag ccataagaaa aagagggaaa
     2041 gaagtatatc ttttcgatgt tgcagataga gtgttgtcga cttattacga ccgtagtttt
     2101 tcagataaag ttgaagaaat actatcaaaa aatggaatac atctttgtct atcagaaagt
     2161 cctaaaaaat atttaggaag aaaaaagatt gaaaaaatag tcactacaag tggtacccaa
     2221 tactctatgg atatggtcgt acaagctata ggatttctat ctaatagtcc aattggggaa
     2281 aaggaactct tgagagatgt tagtggagca tatctaacta atgaatacca gcaaacaaat
     2341 ataaaagata tatatgctat tggggattgt gctacaactt tttttacgtc cattaataga
     2401 agtagtgttg atttttctat ttctaatgct ctaagaacag cttatattgc aactcaacat
     2461 attaacaatc aagattttac accttctggt tcccaattta caaatggctt ttctatattt
     2521 aattacaatt tttacgcagc aggtttgaat ttaaaaacag cacgattatt aagtgagtct
     2581 atagattata tagaaataga agatttcatt agacctgctt ttttgagaaa aaatagtcgt
     2641 gtaaaattaa gaattagctt tgaaaaaaat acaaagagaa tagtaggtgt acaactttgt
     2701 agtcaagaag atttatctgg actattatca atgttttctc ttgctataga agaacaaatt
     2761 acattagaca aattgaaaat gttagattat ttatttcatc ctagttttag ccaaccctat
     2821 aatttt
//
LOCUS       DAFGZE010000162         2756 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.167, whole genome shotgun sequence.
ACCESSION   DAFGZE010000162 DAFGZE010000000
VERSION     DAFGZE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.167"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..222
                     /locus_tag="LTU54_003026"
     CDS             <1..222
                     /locus_tag="LTU54_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297658.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTK
                     KYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            241..1833
                     /locus_tag="LTU54_003027"
     CDS             241..1833
                     /locus_tag="LTU54_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297659.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1845..2180
                     /locus_tag="LTU54_003028"
     CDS             1845..2180
                     /locus_tag="LTU54_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297660.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            2327..2521
                     /locus_tag="LTU54_003029"
     CDS             2327..2521
                     /locus_tag="LTU54_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297661.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            2553..>2756
                     /locus_tag="LTU54_003030"
     CDS             2553..>2756
                     /locus_tag="LTU54_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297662.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQS"
BASE COUNT         1123 a          417 c          547 g          669 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc
      121 aatcaaacca aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc
      181 agagtagatc catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg
      241 atgaataaat cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct
      301 ttttcaacat acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt
      361 acgcccgaag aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt
      421 cctgaaggag aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat
      481 gaagctcaat cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt
      541 accaaatatc tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg
      601 caaagggaac aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag
      661 ccacataaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg
      721 aaaggaagtg atctgaagga gatcaaacaa atcttccaaa gaggacaaca aaaaggctca
      781 atcatttatc aagatgttat ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac
      841 gatcccaaaa cagatacctt agatgaagaa ggtttgaaag cagctggaag aaaaatgatg
      901 gagacattat tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac
      961 cgtaacacta agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa
     1021 ccgtatactg agattgaaaa tggtgtgagt tatatcgtac cgaaggggaa gagaagacaa
     1081 gcaacattag acgcaatgaa aaccaattat gtacatgagt tagaacgttt ctcttatgaa
     1141 aaggatcgaa tgatcggtat ggagaaacga aatttattga cacgaaaaag tgatttgcgt
     1201 aacaccttaa caaaagtagt aaaagaacca gcacgttacg atcgacacgc catgaactta
     1261 ttaaatgaag tatataaaac cctacctgaa aaacgttcgg aatggaatta tggagatgaa
     1321 aatcgaacca agctttcacc atttacaagg ggaaaacttg atgagttaac caaatatgtt
     1381 ttaacaaaag aacctggata tcaagaatat atacagattt caaaagcatt gaaagaagaa
     1441 gccaaaagcc tttatgggga aagcaaaaga gaaacaaaga atgcagaaaa aaacgccctc
     1501 tttgatttaa aaaagcgcac aggaaatgcg attttaacag aactgaagag acaggatagt
     1561 caaataaaac cattaataga aagtgtcaat cagatgcaat ctatttacaa tgaacgatcc
     1621 tttcgaacac tcaaacagtt gccaacaaat gaatacaagt atagttatca agaattcgta
     1681 aagcgtagga aacaattcca acaaagagtt gtcacgcagc gaagcttgcg tcaattaaat
     1741 cgaacaatta atgcacatga agaaaaatac cacgcaagta aagcatatga agagcaacag
     1801 cgaagaatcc aaatggaaca agaaagacaa taggaggaag agaaatgaaa caatatttag
     1861 atcaatggaa agtcatagaa ggatcactaa aagaagaacg aatcgaacaa cttcctgatt
     1921 gtttagaaaa agagcatttg tttcagataa gcgaaatgtt gagaaacgaa cagtttgatc
     1981 caaatcaatt tttagtcgtt gagtactcag ctactggtgt gtattgttgt aatcatgtga
     2041 agggtgaaaa atattttatt attcaagaat acgaaggtaa gttagcgcct tattatacta
     2101 cttgggaaat aaatgaagag ggaatcaata atttcccatg tgaaagcatt gaagagtcta
     2161 tttctctcac ggaatgttaa ataaatagag acgtaaaaga gtcctgaacg tgattgtcca
     2221 ggactctttt tcaatgaatt gttttccgcc aattcactga gtggtgcgat tcaattttat
     2281 tatatcaaaa gaaagtgaga ttccaaagtg aagggaggaa gaagaaatga ctcagacaac
     2341 gattggtgtt gtggttgaaa aaagcaggga taatctcgtt tttgtgacag aaattcaaac
     2401 aggtagagct tttgtagtca cagacaaagc tgcaaaagcg tatcaaaatg gcaatatttt
     2461 aacgttaaat ctgactacga agatgttcgt tgatgcagta gaaaattatc catttgttta
     2521 gacaaacgat attttacaag gaggaaacgg acatggcaga agaaaaagaa ccactagata
     2581 atagtcggtt gggaaagtcc aaaagaaaat tagtacggtt gcaaaacgaa ttaaataatc
     2641 aatttcaaaa gctatttgat caccaaaaat tgacaaatgg gcaaccaatg aatgacaaac
     2701 gaaatggtcg ttcatggttt aaccagcaag aaaaaataga aaataaaata caaagt
//
LOCUS       DAFGZE010000163         2741 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.168, whole genome shotgun sequence.
ACCESSION   DAFGZE010000163 DAFGZE010000000
VERSION     DAFGZE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.168"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(575..1132)
                     /locus_tag="LTU54_003031"
     CDS             complement(575..1132)
                     /locus_tag="LTU54_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297663.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1311..1922)
                     /locus_tag="LTU54_003032"
     CDS             complement(1311..1922)
                     /locus_tag="LTU54_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297664.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2035..2292)
                     /locus_tag="LTU54_003033"
     CDS             complement(2035..2292)
                     /locus_tag="LTU54_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297665.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
BASE COUNT          818 a          581 c          358 g          984 t
ORIGIN      
        1 ccttattccg aataaatggt gtccaaaagc ttgcatctgc cagattatct catttcttat
       61 gacaagctaa gcaaattatc tccaccaaat aatttattca tatttttgac ttgcgcatac
      121 taactaattc ttaaattttc atacaaaatt tctcaatttc ccttaacgag taatatttat
      181 gtacggtgat tctcactagt atcccaatca ctttttaagt tatcccgatt tcaattctac
      241 ttcaaaaata gttgctttta tgtatgctgt tagttgatta gatggtcatt tgttgagcta
      301 ggtcgtttat tttaaacaag ttgtttcttt agcttatacc tatctcgtct accaaacgca
      361 agatggcaaa cacaaatgca tacgcatctg tatttgccac tttgattcgt ataaagctaa
      421 caactattaa ctcctctcag ctaatagttg ctctcttttt tattcagatt catttttgct
      481 gatcacagcc tagtatttca atggttttga acactaaacc aaattgttcc attacaacat
      541 tcactggctg atgaaatgga ttagtagatt tcgattattc aaagcgattt tctattggtg
      601 gatcgtgttc ccaactaata agacggattc caaacctatc gtacagatct tctaaaccac
      661 ttataaattt tgcacctatt tcaggtgttt tattgcgtgg atattctatt gttacgccat
      721 taaatggata ccaattagga ttaggctctc tttttttctg tacatctccc atagaatagt
      781 tagcactttc tgggacataa tatattcttc gaccatcctc taattcatct aataagatat
      841 ttcgtgatcg tctatcactt gctaaatgta gacgccacgt gcgataccat gccatgctac
      901 ctgcatctgc agaaaatcca tcaaataatg caacaattgt atcttctatc acaatccttt
      961 ccccatctac ttcatattct aatctaaaat caaactcccc tcgggtaatt tctggtcttg
     1021 gtggcttagg acccaatatt attctactta aaaccgcatt cgttcccaga tgaaacacag
     1081 ctataacaac aataatgatt aatattttgt ttctatttga taaatttctc attattcgca
     1141 catcacctct acattctcac atattagttc gtaatactcc tttaatatca aaatatctac
     1201 tggtcgtaat aatattcgct atgaattcct actactcgta taagaaaggc caaatcatca
     1261 gcttttactt gctgatgatt tggcttgttt ctatttatcg taattgtttt ctaatcacca
     1321 aaattgttaa caatcggttc agatatttcc cagttgatta aggtaattcc gtattgtgtc
     1381 aaaacttctt gcgcttctcc gaaattaagt acagttctta tgtatccact ttctcctaat
     1441 ataaagtcta cctctctttt aaaaccttcc tcgagatttc taagggctat atgtggtttt
     1501 aatgacattg attgtacatt ttcccccata taatattgcg cacttcctcg actaaaatag
     1561 attcctagat cgtccccaaa aggtagatct tctctaccac tagctaatct acttctccat
     1621 cttcgttctc ttttcataga acctgcgctg aactcacacc cgttaaactc agctatgaca
     1681 gtatcttcaa ttatgttaat ctgtccatct agttcgtatt caatcctaaa tggaaattct
     1741 ccataggtga tttccggttc gggtatttta ggtgagatta atgcaaagaa aggaaatatt
     1801 aagcttggca acatcaaaaa aactcctcct atgatcacaa ttcccgatgt aatccaaaat
     1861 ataatttgta caattctcat tgatccattg tattccattt tattccccct attactattc
     1921 atatccgcct atctatatcg atcttccaat gcagagataa ttgcttccat accttgattt
     1981 tttactgagt tacctatgcg agatatcatt ctccactaag gtgcatcata ttgtctatct
     2041 aacatgatta attctccaat aggtgtatac tcctccaaaa acaacattta atgcatctcc
     2101 ctcgacaaca taagctacca tgtttgctgt atatgtgttt gagtctaatt cataagcaga
     2161 taaatttact gtcgctggat taaatataat cgcattcgta tttatagcta ctgaatttgc
     2221 tgcttcagca ccaccttttg aatgtccgat cattgtgatt tcaatagtag gatttacgtg
     2281 gacagccctc accgtttacc ctgaccatta tagaaatcgc ttactttttt gaactacgca
     2341 acaaaggata caatgctcct aacttcactg tctccatttt tgtccactaa ggaatataaa
     2401 aaataggcat taattcattt aattcggtca caacacgtta ccttttaaat cattatactg
     2461 tatctcatac attattatca ataaactatt ataaaaatgc aaaaaatgct ttatcaatct
     2521 attttgtaat tggtcataat atcattgaaa tcataccctg tatcgatttt tcctatgaat
     2581 ctcgtctatt tgtgattgac gaaaaagagg caagacagac actggaatgg taattttacc
     2641 cactattttg ctccaactat ttgtaatcgt acccactttt gaaataccga gatgatcatt
     2701 ttaattgata tgctgattcc caagcaaaac cccaagaaat t
//
LOCUS       DAFGZE010000164         2705 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.169, whole genome shotgun sequence.
ACCESSION   DAFGZE010000164 DAFGZE010000000
VERSION     DAFGZE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.169"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            69..533
                     /locus_tag="LTU54_003034"
     CDS             69..533
                     /locus_tag="LTU54_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBL8297666.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            547..1296
                     /locus_tag="LTU54_003035"
     CDS             547..1296
                     /locus_tag="LTU54_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBL8297667.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            1296..2126
                     /locus_tag="LTU54_003036"
     CDS             1296..2126
                     /locus_tag="LTU54_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBL8297668.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            2126..>2705
                     /locus_tag="LTU54_003037"
     CDS             2126..>2705
                     /locus_tag="LTU54_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBL8297669.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVS"
BASE COUNT          903 a          351 c          526 g          925 t
ORIGIN      
        1 tttaaaagat gtcagttaag gtggcaattt ctattctata aattttaaaa attgaaggag
       61 gaaatagaat gattaaatta gtgcgtgtag accacagatt acttcatgga caagtaatat
      121 tttcttggac taaacagtat gatattaacc atattattgt ggctgataac aaagttccag
      181 gagatccaat gtctgtgatg gctttgacaa ttgctaaacc tgctgattgc aaattagaca
      241 ttgttaaaat tagtgatgtt aaagggatta tggaaacatc cccaaatgat aattacatga
      301 ttttaattaa aggacctaag gaagcttttg aattagttca aaatttacca gaaattaaag
      361 aaattaattt tggcggtgtg tctaagaaaa gcgagtccaa acaatatgga aaggcagtct
      421 ttttaactcc tgaagaatta gaagccacaa aaaaaattct tgatcaagaa gtgaaaatat
      481 ttgtgcaaca agtcccatct tcaagtattg aaaatgtaga ttttagtaag taagggggga
      541 aaataaatgt tattaaaagc cattttatta ggtttagttg gtgtggtagc tgttattgac
      601 tccaggttaa ttggtcgaca aaatattggt agaccactaa ttttgagtac acttgccgga
      661 cttgtgctag gtgacttgca tacagggatt gttcttgggg catctttaga attaatttct
      721 atgggattcg tctcaattgg ggcggctgga cctcctaata tgcaacttgg tagtattatt
      781 gccactgctt ttgcaatttt aactaatagt agtacagaaa cagccttaac aatagctatc
      841 ccagtggctg tagctggtga atttatgagt attatcatgc gcatgataat tgctcaattt
      901 ggacatgctg cagataaagc cattgaaaat aataaattta gaaaagctca aattattcat
      961 atttattggt catttatttt taatgctttt gtttacttta ttccaatctt tttaacagta
     1021 tattttggtg ctgacgttgt ggcagattta gtagataaaa ttccagaaat gattacagac
     1081 gccttgactg tctctggaaa tatgctttcc gcattagggt ttgcaatgct attaagcaca
     1141 atgttgagta aaaaattata tccttacttt atttttggat tctttattgt tgcctattca
     1201 ggtctttcac taattggcgt gacaattttt gcagctctga ttgcctttgt aatggatcaa
     1261 gttaaatatg gaaaaagaga agaggcgcat ggttaatgga aaatagtaca gaaaaaaaac
     1321 gtttaatcac aaaaaaagat cttaataaga tgtgtcttcg ttcatggaca atcatgtgtt
     1381 cgtggaatta tgaaagacaa atgcatatgg gatttatgta tgggatggcg ccaattttaa
     1441 ataaagttta taaaaatgat gaagagaaaa aaagagaagc ttatactcgt catatggcat
     1501 tttttaactg tacgcctcaa atgacttcat ttattatggg actttgtgct tctatggaag
     1561 aacaaaatgc tcaagcagaa gaaggaaact ttaatactga atctatctcc atgattaaaa
     1621 ccagtttaat gggtcctttt gctggaattg gtgattcatt tttccaaggt actttaagaa
     1681 ttattacatt tgggattgga ttgtcttttg ctaagcaagg tagtatttta ggaccgattt
     1741 tagctgttgg cctttttgct ataccttcta ttttagctgt tttttacggc ggtaaattag
     1801 gatatgagtc tggcaataaa taccttacta aattgtatca agaaggtctt atggatcgag
     1861 taatgcaatt tgcttctatc attggtttgg ctgtagtagg tggaatggtg gctagcatgg
     1921 tgccaattgt caccccttta aaattcacta cagggggaac agaacttgtt ttacaagact
     1981 tacttgattc cattattcct ggattattac cacttgtatg tactttggga atatataaat
     2041 tgattaagaa aaatgttagt acaaatatgc tcttattagg ttgtattttg gcagggtttg
     2101 ttttaagtat aataggtatc ttataatgat taaggctcta atttttgata tggacggagt
     2161 tttaattgat agcgagatta tgtatcaaaa gtgggtattt aattttttaa aacaagaaaa
     2221 tttaagctat cctttgtatt cttatcaaaa aaagattgga actacagcaa gtatttttga
     2281 tgatatagaa aagtacaatg agggatgtaa cagtttagaa ttgaagggaa aatttactaa
     2341 atattggaaa tcgcaaaaga ttgattactt ggatattttt cctaatatac taattgaaca
     2401 gcttgaatgg ttaaaaaaga aaaaaataaa aatggcaatt gcttcttcca gtccaatgga
     2461 tactataaat gaagttgtta ccacttgtaa aatagagtca tattttgatt gtttaattag
     2521 cggaagagat ttgccagaaa gtaaacctaa tcctactata ttccttaaag cttcagaaca
     2581 gctcagaatt ccagttgaag aatgtctggt gatagaagat tcttataatg gtataaaagc
     2641 tggtaaaaga gcaaatatga aggttcttgc aattaaagat aaacgttttt ctcaagatgt
     2701 aagtc
//
LOCUS       DAFGZE010000165         2685 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.170, whole genome shotgun sequence.
ACCESSION   DAFGZE010000165 DAFGZE010000000
VERSION     DAFGZE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.170"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            146..1549
                     /locus_tag="LTU54_003038"
     CDS             146..1549
                     /locus_tag="LTU54_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBL8297670.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1898..2530
                     /locus_tag="LTU54_003039"
     CDS             1898..2530
                     /locus_tag="LTU54_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HBL8297671.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         1020 a          379 c          519 g          767 t
ORIGIN      
        1 aaaggcagta acaggattaa acattgaaac ataatcgtta ctaaatgaaa tcaagaaata
       61 tactgtatta ctcaattcat tgaaaatatt tatcataatt tttaatagaa aacactatta
      121 aagagaaaaa agagaggtaa atcaaatgaa aaaatcttta aaacttgcat cagcagttat
      181 attaatgtcc caactcctta tagcaacacc tttccatgtg ttggcaagcg aaaattcaac
      241 atacaaaatt gcaaaagaaa gaaccgagac gactgaaatg agtaattccg atgtaacagc
      301 agagagtagt gaacttttga caacttcatc aagtgaaagt agtacgactt caaatgccga
      361 gtcttcaaca acttcatcaa gtgaaagtag tacgacttca aatgccgagt cttcaacaac
      421 ttcgtcaagt gaaagtagta cgatttcaag taccgaatct tcaacaactt caagtagtac
      481 aagtgaaata acttcttcga aagtgacaaa accaaaccat atagaatctt caaaaaagga
      541 agcgccaaaa acggaaggac aaacaaaaac tttttcgaca ccacagcaaa tagaatcaac
      601 agaatctttc caatcacaac caatactatc tggtaatgaa gaaattcatt ttgacaaaaa
      661 tgaatctgta gaaaatttta ttatgaaaat tggtgagtct gcgagaaaaa ttggtcagaa
      721 atatgattta tatgcttctg ttatgattgc acaagcgatc ttagaatctg ccagtggtca
      781 aagccagtta gctcaggcac cgaattacaa tttatttggg ataaaaggaa cttataatgg
      841 aaactttgtc attatggtaa caaacgaaga tttgggtaat ggaacgcttt atacgaccca
      901 atcaaaattt agagtgtatg aaaactatga ggaaagcttt gaagattatg ccaaattatt
      961 aactaaggga attagtggaa ataaagattt ttatgcagga gcattaaaag ctaatagtaa
     1021 aacatatcga gaagcaacta aattccttac aggacggtat gcaacagata cacaatacta
     1081 tttgaagcta aatgaattaa ttaaaaccta cgatttaacc aactatgata aagaagttaa
     1141 aggtccacaa atgagtaaga ctggatacca agtgcctatt cataattatt caataaccag
     1201 tttgtttgga aatcgtggaa atgaatttca ccgaggactt gatatggcag ctacacaggg
     1261 tgagccgatt catgcagtta aagctggtaa ggttgttaaa gcagagtttc actactcttg
     1321 gggaaattac gtggtgatac aacacgaaga tggatcaacc gcgttatatg ctcaccagca
     1381 acaataccta gtaaaaactg gagataatgt tttgcaagga caaataattg gctatgtagg
     1441 aagtactggg aatagtactg gcccacacct tcatttggaa atttgcaaag atagtagtct
     1501 ttctcagagc atgttagttg atccgaaaac cttattattc ggagactaaa aaaggtaatc
     1561 aatctgacaa agtaacaaga aaatggttgg attcttagac ctttgatcag gatattgaga
     1621 ctggaattga tatctcgatt acgatgggtt tgacaaataa aacaagtcca ttatcgaata
     1681 tcgagtgtat tattgccgtc tttatgctca tattttgagc aggtttcact agatgaaaac
     1741 aatttatcta tcttgaggat ttcgtgtacg tatcgttcag tcttgtattg taaattagtc
     1801 ataaaatcag actaagaaaa attggaaata tttttttctg atttattaaa agttaattga
     1861 aattatatgg tacttctttc ttgggagggt aggtgcagtg aaggaaagat atggtcacct
     1921 tactgttgtt gaggagatta ctcataatgg atataaaaaa tatcgttgcc tatgtgactg
     1981 tgggaatgaa acgattgtat atcgatcaaa cttactaaca ggtagaacca aaagttgtgg
     2041 atgtggaaat tataaaaata ggcataaata taaaaatttg acaaataaaa cttttggtaa
     2101 gttagttgca atcaagccaa ctaaactgcg aagtgaagga actgttgttt gggaatgtca
     2161 atgtctgtgt gggagaaaaa agttagtatc ggcaaagcga cttctaaaag gtgatgtaaa
     2221 aagctgtgga tgtttgaaat caaaaaagca tcacattgaa aataaaaaat tcggattact
     2281 tactgcaaag tatcctttgg acaataactg ttcatggaaa acaaaatggg tttgtcattg
     2341 tgagtgtggc agtatgtgtg agataagtta tagtaactta ttttttggac atactcaaag
     2401 ttgtggatgt ttaaaaaggc aagaaacaag gacatgtgtt gaagggacag taatagagtg
     2461 tctaacgtca aaattaccga aaaataatac aagtggtgtt aagggagttt atcgttctaa
     2521 agagaaatag attgcgtata taactttaaa aaaaaagagg tactatttag gatcgtatga
     2581 ttctattaag agtgcagctc aagctagaaa agatgctgaa aataaattgt ttcaaccttt
     2641 gattgacaaa tatcgaggta atcattaatt attgtggata ataat
//
LOCUS       DAFGZE010000166         2523 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.171, whole genome shotgun sequence.
ACCESSION   DAFGZE010000166 DAFGZE010000000
VERSION     DAFGZE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.171"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..222
                     /locus_tag="LTU54_003040"
     CDS             <1..222
                     /locus_tag="LTU54_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297672.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVEGKIKANQTK
                     KYSPKIKKPINESVHARVDPFDPTKFLKR"
     gene            242..1834
                     /locus_tag="LTU54_003041"
     CDS             242..1834
                     /locus_tag="LTU54_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297673.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPDE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1855..2190
                     /locus_tag="LTU54_003042"
     CDS             1855..2190
                     /locus_tag="LTU54_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297674.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2336..>2523
                     /locus_tag="LTU54_003043"
     CDS             2336..>2523
                     /locus_tag="LTU54_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297675.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT          997 a          394 c          525 g          607 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgtcta tcaagaagca aaagagcgtg tggaagggaa aataaaagcg
      121 aatcaaacga aaaagtattc tcctaagatc aagaaaccaa taaatgaatc tgttcatgcc
      181 agagtagacc cgtttgatcc aacaaagttt ttaaaaagat aaaaagtaga aggggaacat
      241 gatgaataaa tcgcctgcag tcattctcac ttgtcaattt accttgccca ataaaaaatc
      301 tttttcaaca tacattgatt atatgacaag agaaaaagcg ctggaagaaa aggaacgacg
      361 cacgccggac gaagaacagg aactgcaacg aatcaagaat gctttagagg atttttatat
      421 tcctgaagga gaaacttttt cggagagtaa gacagataaa gaattcagtg aaacgtcgaa
      481 tgaagctcaa tcgttgatgg aacatggaat caattttgac aagttagagg aacaagattt
      541 taccaaatat ctttcttata tgacccgtca ctatgcgtta gaacaaaaga aagaactgac
      601 gcaaagggaa caaaaagaat accaacggtt gaaccaagca atgaaaaaat atcaaacaga
      661 gccacataaa aaagcttcag gaaatcttcc tggagttttt tctatggctt cagacgaagt
      721 gaaaggaagt gatctgaagg agatcaaaca aatcttccaa agagggcaac aaaaaggctc
      781 aatcatttat caagatgttg tctctcacga caattcgtat cttgaaaaaa tgggcttgta
      841 cgatcccaaa acagatacct tagatgaaga aggattaaaa gcagctggaa gaaaaatgat
      901 ggagacatta ttcgagaaag aacaattgaa tgaaacaggt tactggatgg caacgattca
      961 ccgtaacact aagcatatcc atattcattt tgcagttgtt gaaaaagaaa atacgcgtaa
     1021 accttacacg gaggttgaaa atggtgtgag ttatatcgta ccgaaaggta agagaagaca
     1081 agcaacatta gacgcaatga agaccaatta tgtacatgag ttagaacgtt tttcttatga
     1141 aaaggatcga atgattggta tggagaaacg aaatttattg acgcggaaaa gtgatttgcg
     1201 taacacctta acaaaagtgg taaaagagcc agcgcgttac gatcgacacg caatgaactt
     1261 attaaatgaa gtatataaaa ccctacctga caaacgttcg gaatggcatt atggggatga
     1321 aaaacgaacc aaactttcac catacacaag agagaaactc gatgagttaa ccaaatatgt
     1381 gttaacaaaa gaacctgaat atcaagaata tatacagatt tcaaaatcat tgaaagaaga
     1441 agcaaaaagt ctttatgggg aaagcaagag agaatcaaag gatgcagaaa aaaatgctct
     1501 ctttgatttg aagaagcgca caggaaatgc cattctaaca gaactgaaga aacaggatag
     1561 tcaaataaaa cctttaatag aaagtatgga tcagatgcaa tctatttaca atgaacgatc
     1621 ctttcgaacg cttaaacagt taccaacgaa tgaatacaaa tatagttacg aagaattcat
     1681 gaagcgcagg aaacaattcc aacaaagagt tgtcacgcaa cggagtttgc gtcaattaaa
     1741 tcgaacgatt aatgcccatg aagaaaaata ccacgcaagt aaagcctacg aagagcaaca
     1801 acgaagaatt caaatggaac aagaaagaca gtaaagacag taggaggaat agcaatgaaa
     1861 caatatttag atcaatggaa agttatagaa ggatcattaa gagaagaacg aatcgaacaa
     1921 cttcccgatt gtttagaaaa agagcatttg tttcagataa gagaaatgtt gagaaacgaa
     1981 cagtttgatc caaatcaatt tttagtcgtt gagtactcag ctactggtgt gtactgttgt
     2041 aatcatgtga agggtgaaaa atattttatc attcaagaat atgagggcaa gttagcgcct
     2101 tattatacta cttgggaaat gaatgaagag gggatcaata acttcccgtg taaaagcatt
     2161 gaagagtcta tttcactcac agaatgttag ttgttaagtg aataaaagaa gtaaaagagt
     2221 cctgaacgta attgtccagg actctttttt tcaatgaatt gttttccgcc aattcaatga
     2281 gtggtgcgat ttgcgactac attatatcaa aagaaagaga agggaggaag aagaaatggc
     2341 ttattcaacg attggagttg ttgttgaaaa aagcagagac aatctcgttt ttgtgacaga
     2401 aattcaaacg ggcagagctt ttgtcgtcac agacaaagcg gcaaaagcgt atcaaagtgg
     2461 cgatatttta acgttaaatc tgactacgaa gacgttcgtc gatgcggcag aagattatcc
     2521 att
//
LOCUS       DAFGZE010000167         2510 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.172, whole genome shotgun sequence.
ACCESSION   DAFGZE010000167 DAFGZE010000000
VERSION     DAFGZE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.172"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            209..2362
                     /locus_tag="LTU54_003044"
     CDS             209..2362
                     /locus_tag="LTU54_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBL8297676.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          866 a          431 c          548 g          665 t
ORIGIN      
        1 cttaagaaaa cataattttc aaaaataagt ttatttttta atgaaaaaaa tagttggata
       61 tgtaacttta agtgattgct gtgaaacaaa aagttgattt tatcttgaaa ttaggtaaag
      121 aatttcttgc tccgcccatt ttttcttttt tattaagaag tttttgggta caatgaagca
      181 tgaatgtaaa taggcggaag gataggaaat gatgaaagat tttatgaaaa aaatattaaa
      241 tacttcttgg ttagaaaaaa taagaaaagg tgcacaagaa cctaagaaca aaacgtttcg
      301 ccggtcccat gttccttttc gcttaaattt tctttttttc attatattta cgttatttgt
      361 tgcgttgatt gctagattgg gttatttgca gatcgtgaac ggcgaagaga tggaggcgcg
      421 agtgaagtct acttctacga ttacggtcca agaaagtact cctcgcggaa tgatctacga
      481 ctccacagga aaagctttag ttacgaataa agcgaatcag gccatcacat atacgagagg
      541 aacacaagtt acggctaaag atttgcttga aatcgcaacg aagctgaact cgctgatcga
      601 tgttccggta gatgaaaatc tgacagatag agataaaaaa gatttttggc tggctgatcc
      661 caaacatttg aaagaagcca caaaaagact ttcggcaaaa gaaaagcaat tagggaacag
      721 cgaacaatat agtgcgatgg tcaataaagt cacagctgat gagattaatt taaacgagga
      781 gcaattaaaa attgctacca tctttaaacg gatgaatagt gcatatgaat taaacactgt
      841 ctatatcaaa aactctggtg ttactgatca agagttagct gttgtggcag aacgcgcttc
      901 tgaacttcca ggagtttcga ctggcacaga ctggacacga gaatataatg cagcaagttc
      961 actgaaaagt attcttggca gtgtgacgac tgaaaagcaa ggacttccag cagatgaagc
     1021 agagaaatac ctagcaaaag gttattctcg gaatgaccgt gtaggacaaa gttatttgga
     1081 aaaacaatat gaagatgtcc tgcaaggaac caaaacccaa tatgaagttt cccttgataa
     1141 tgaaggaaat gtttcgaatc aaaaagaaat cttctctggg gaaaaaggtt ctaatttaat
     1201 gttatctatg aatgcagaat tccaatcaaa agtagaagaa attttgaaaa ggaattatca
     1261 aacattgatc aacaacggca aagcacagta ttcgcctggt gcttatgcag tggctatgaa
     1321 tcctcagaca ggagaagtgc tagcaatgac tggtttcagt catgaacaag gttccaaaga
     1381 aatcaccgag aatgctttag gcacgattac tagcgccttt gatccagggt ctgtagtcaa
     1441 ggctggtact ttgacagcag gctgggcaag taacgcgatc agcggaaatc aagttttgat
     1501 tgatgagccg attcgtctgc aaggtgcttc agaaaaaagc tctgtcttta atcgaagcgg
     1561 acaagttgct cttgatgcag tcaaagcatc ggaattgtct tctaatacgt atatgatcaa
     1621 agtggcgtta aagatgttag gactagacta tacacctgga atgggactac ctagtttaga
     1681 tgaagaagct aaagcttatc agcaattgag agactctttc aaagagtttg gtcttggtac
     1741 aacgactggg atcgatttgc cgaatgaatc tcctgggatc tcacgttctg ttgattatat
     1801 gaagaagttc aatgcggata atggcaaaga atggtatgct ccaggtaact ttaccgattt
     1861 ggcatttgga cagtttgaca catatacacc aatccaatta gctcaatatg cttcaacagt
     1921 cgctaatggc gggaaacgag tacagccgca cctagtcaaa gcgatctatg gcaatgacga
     1981 aaatggaaac ttgggtgaag tgaagaaaga aattggcaca acagtagaaa atacggtcaa
     2041 tatttctgca gaaaatatgg cgatcctccg tgaaggattc caccaagttg tccatggtac
     2101 cgatccttac acaacagcca aaccattagc cagtgcgaaa atggaccttt ccgcaaaaac
     2161 agggactgca gaaacggttg cggaaggtca tccagatatt gcgacagtca atagtaatat
     2221 cgtggcatac ggaccaacag acaatccgga aattgccatc agtgttgtct tgccgaactt
     2281 attagacgaa caagaccaca taaacttgac gattgctaaa gaaataatgg acgcgtatta
     2341 tgatatgttc atggcaaaat aaaaaagagg tcgtgaaaaa gccgcttagt ttcgagtatg
     2401 aagaaaaatt ttgtgaaaat agcttctcac atttttgctt aaatttgact tcataccgag
     2461 aaactggctt ttgaacaccg tttactcggc aaaaaaggtt gtgacaaaaa
//
LOCUS       DAFGZE010000168         2510 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.173, whole genome shotgun sequence.
ACCESSION   DAFGZE010000168 DAFGZE010000000
VERSION     DAFGZE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.173"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(951..1469)
                     /locus_tag="LTU54_003045"
     CDS             complement(951..1469)
                     /locus_tag="LTU54_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HBL8297677.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT          819 a          457 c          361 g          873 t
ORIGIN      
        1 ttatgaataa tcgcaaattt acccctatac tactagtttg taagttagat taataaaaat
       61 actaaagtta tttgttcttt tattaaatac acctacaact tataaattga tggtatttag
      121 ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat tgctactcaa
      181 tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt
      241 tacatcttgc agaaatcggt actgggatta aactaagcaa taaaaactaa tgttcccctc
      301 attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt tcaccttcag
      361 gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta ataggatagg
      421 cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt atcgtatgtt
      481 ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt tgaatcgttc
      541 ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg acttgcctta
      601 cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac tatcggcggt
      661 ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa gtccctttgt
      721 agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat ctgttggcca
      781 accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga gtgcacttgc
      841 attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg ttcttactgt
      901 taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat ttacaaagct
      961 aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac tgtatttaca
     1021 tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc ccattttctt
     1081 ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt aatatcatca
     1141 atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata ggtatttgta
     1201 aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg cgttttaaca
     1261 gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca accaacttga
     1321 tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc cttcaattct
     1381 tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat ttcttttttt
     1441 aattcttctt tatttttata tgttctcatt atattacctc cataaattcc taattgtctc
     1501 ctttaattca tctataattt catccacgat attttaatcc gttctacaat gcatatctga
     1561 tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa cgtctcatct
     1621 aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct tttgtagtaa
     1681 aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt aactactatc
     1741 aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag ttaagtgagg
     1801 atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa atgctttata
     1861 taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa
     1921 aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa aaacgaaatg
     1981 atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc ctcgctcgac
     2041 gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt gaagcttttt
     2101 atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt cttctagggt
     2161 gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt actaaaatta
     2221 tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca agctggtgtt
     2281 atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata gaaaaccgat
     2341 actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc tgaaacttca
     2401 ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta ttttatcaaa
     2461 aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc
//
LOCUS       DAFGZE010000169         2473 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.174, whole genome shotgun sequence.
ACCESSION   DAFGZE010000169 DAFGZE010000000
VERSION     DAFGZE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.174"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..1169)
                     /locus_tag="LTU54_003046"
     CDS             complement(<1..1169)
                     /locus_tag="LTU54_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL8297678.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWG"
     gene            complement(1222..1893)
                     /locus_tag="LTU54_003047"
     CDS             complement(1222..1893)
                     /locus_tag="LTU54_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HBL8297679.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(1953..2162)
                     /locus_tag="LTU54_003048"
     CDS             complement(1953..2162)
                     /locus_tag="LTU54_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297680.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(2229..2468)
                     /locus_tag="LTU54_003049"
     CDS             complement(2229..2468)
                     /locus_tag="LTU54_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297681.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT          648 a          556 c          363 g          906 t
ORIGIN      
        1 tctccccatt taaatacact gtaaggagaa gtttcgcttt tcacaccgtt tactgtgata
       61 ccgtctgcgc cacctgcaat gtctgttgga atatgaatcg ttccttgata atttactgcg
      121 tcaccaattt ggaaagaatg ttcttgtcct gttccatttg ttactgtttt atcgatcact
      181 ggtgctttat agtttacgta tgaagctttt aagtcacttg tcacgccatc attcgtaatt
      241 tcaaaggtta atttattgtg cgcatcgttg cgagcgtctg cgcctaataa atattgatct
      301 gctgttggtt cttgatctga tcccactaca acttccgaag gaacttccac aaaatagtat
      361 tttcctaacg tcaaatcagt cgcagtcagc tttccttgat catccgatac aagatcaccc
      421 aattttgttc cttttccagg ttcgcccttg tataactcaa attttgcccc ttttaaaagg
      481 gtttgtttgc tttggtctgc gccatcgtca ccaaatttcg ttaattcaaa actcaactta
      541 gaaacaatgt tcttaggata caaatgaata ttttttaaaa atcctgatcc tgttttatct
      601 gtcattggcg caatcacaac cattggtttt gctttttgtg tgactaatcc agcagcggat
      661 ttactttcat aaaccacata tacattccct ttggaattga cataggctgg ttgatcagta
      721 aaagtgatct caccattttc atctacagct gaagtaattt tgttggttgc attcttgacg
      781 tactcactgt tagatccctt gtcttctaca tcttgtacaa tctcgtccac tttcgcgttg
      841 gttagatcag aatcttctct cggtaatact tgatccgtaa tatttcctaa cgtaaactca
      901 acatcaccat aatctgcctt gttgtatttc gttacacctg taggatattg atcgtgcgta
      961 taaccatcgt ttttgatccc gtcaatatca acattcaatt gcgttccttg atcgtacatt
     1021 aatttatgaa ccgtgatatt gaccttctct ggtggtgttt tttgcgtgac atcttctgca
     1081 tgtacttgat acccctgaaa gcagcctgca atgattccta acattccaac aagcattcca
     1141 gcttttttta cataccgatt tattgccatt ttttactctc ctcatttcat atttcaacga
     1201 ctcgcgttga ttaaaacctt gttattgtag ctctccctgt accacgtatc tttcttttcc
     1261 ttgtgacgta taaacacaag taaacaacgt taataagggt ttctctcctt tctttggcaa
     1321 agacaagaaa gagttctctt caggaacgcc atcgattaat ttaaagtacc caggtacgat
     1381 aaatttacgc gttaccttgt aacgatagac gccatcgtta ctaactaagt ccatcacctc
     1441 ccctaatgac aacttttgaa tatcagaaaa taaaacacct ggcatttcca tgttatgacc
     1501 tgctaggaca tagtttcctt tgcccatttc ggcatcttca taaaaataag tagccgctcc
     1561 aagactcaaa gtgtattgat ttgccccttt aaaaattggc aatgctaaac caagtgcagg
     1621 aatctgaatt tgtgcaaata cttgtttatt gacgcccatt tcgattgctt ctttttgtat
     1681 ggacaacatt tgccccaaag acatatcatt attgacgttc gttgcatcct ccttataact
     1741 ctttgcatgt gcattgattt gatctacggg catctttttt tctatatttt gcttaaacac
     1801 ctctccttca tgtttttggt agctaacata gccaaacaca ctgcaaaaga cgattaaagt
     1861 gactcctaaa aaaccaaata accatttttt catccatctg cctcctttca gacaaaacaa
     1921 aaaaactttt tctcatttcc atagaaaaag ttttaaaaat gaattctata agttcgtctc
     1981 aactgttcaa agtttttcat tgaacctaac aaacaagctt tctcttttcc tgttaactgc
     2041 atatcattga tatattgcgc gacagataat cttttaaggt gtttttgctt catcgttttt
     2101 agtaactttt cttccatttc tttcgacact ttttttctgt tttcgatttc ttcataattc
     2161 atgagttttc ctaccttcct tgttttttgc aaaaataaaa aagcctatga tcacatacgc
     2221 tctacacttc agtcttgggt tatctttttc tttcgtttaa ttactggccg tgctggtttc
     2281 aaatccttga ttccattatg ggttctccaa taatcgaatt cttttcgcaa tctttctttt
     2341 tccatatctg ttcctttaga taaggctttg aatacttctt taggtgaata accacgagct
     2401 aacagctgta cataccttcc ttttgtcaaa gtatccatat ccatcgcctc taaaactggt
     2461 cgcgccatct act
//
LOCUS       DAFGZE010000170         2398 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.175, whole genome shotgun sequence.
ACCESSION   DAFGZE010000170 DAFGZE010000000
VERSION     DAFGZE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.175"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            207..2069
                     /locus_tag="LTU54_003050"
     CDS             207..2069
                     /locus_tag="LTU54_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8297682.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="LTU54_003051"
     CDS             2069..>2398
                     /locus_tag="LTU54_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8297683.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DAFGZE010000171         2356 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.176, whole genome shotgun sequence.
ACCESSION   DAFGZE010000171 DAFGZE010000000
VERSION     DAFGZE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.176"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..434)
                     /locus_tag="LTU54_003052"
     CDS             complement(<1..434)
                     /locus_tag="LTU54_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta(1,3)galactosyltransferase EpsH"
                     /protein_id="HBL8297684.1"
                     /translation="MIFITLGSQKFQFDRLLKAIDDLIEKGVIIESTFAQSGYSDYKP
                     LNYSYKAFLNRDEFTETMQKAEMVITHGGTGAIIGAVKKGKRVIAVPRLAKYGEHVDD
                     HQLQLIRQFDEMNIIYPCYELENLEDAVKETRVRMFNKYVSNT"
     gene            complement(451..912)
                     /locus_tag="LTU54_003053"
     CDS             complement(451..912)
                     /locus_tag="LTU54_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBL8297685.1"
                     /translation="MNKKICFAASSGGHYEQLMMLKPLMEKYESFVITEQTEYSAESK
                     GEKTYYMRQVNRKEKFFLCTILINLFKSIIIYIKEKPDLVICTGVLAMIPICLITKMM
                     GKKLIYIESFAKVTSPTQTGKLMYHFADQFYVQWETMLQFYPNAIYLGGIY"
     gene            complement(926..2176)
                     /locus_tag="LTU54_003054"
     CDS             complement(926..2176)
                     /locus_tag="LTU54_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBL8297686.1"
                     /translation="MKKKYNIAVAGTGYVGLSIATLLAQHHQVTAVDIIPEKIELINK
                     RKSPIQDEYIEKYLTEKELDLTATLDARSAYKSADFVVIATPTNYDSRTQHFDTSAVE
                     SVIEVVMDVNPDTIMVIKSTIPVGYTESVRETTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMTDERLLNAAHVFATLLQKSAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPGNLIEAIVESNRTRKDFIADRVLQMAGYYNYAEYNQYDVNRENPVLIGIYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPALKDGETFFGSKVINDLEQFKAMCDSII
                     ANRYDTCLDDIKEKVYTRDIFQRD"
BASE COUNT          720 a          499 c          353 g          784 t
ORIGIN      
        1 gtgttagata catacttgtt aaacatccga actcttgttt ctttaacggc atcttctaaa
       61 ttttctaatt cataacaagg atagataata ttcatctcat caaattgtct aattaactgc
      121 agttggtgat catcgacatg ctcaccatac ttagccaatc gaggaacagc aatgaccctt
      181 ttaccttttt ttacggcccc aattatagca cctgtacctc catgagtaat caccatttct
      241 gctttttgca tagtttcagt aaactcatcc ctatttagaa atgctttata ggaataattt
      301 agtggtttat aatcactata accactctgt gcaaatgtac tttcgattat tactcctttt
      361 tcaattaaat catcaatagc ttttaacaat ctatcaaatt gaaatttttg tgaacctaat
      421 gttataaata tcattttttt ctcctattat ttaatatatc ccacccaaat aaatagcatt
      481 aggataaaat tgtagcatag tttcccactg aacataaaat tgatctgcaa aatgatacat
      541 caattttcct gtctgagtag ggctggttac ttttgcaaac gattcaatat aaatcagttt
      601 ttttcccatc atttttgtta ttaagcagat agggatcata gctaaaacac ctgtacaaat
      661 cacgagatcc ggtttttcct ttatatatat aataattgat ttgaataaat taataagtat
      721 agtacacaaa aaaaactttt cttttctatt tacttgtctc atgtagtacg ttttttcgcc
      781 ttttgactca gctgaatatt ctgtctgttc tgttataaca aaactctcat acttctccat
      841 cagtggtttt aacatcatta actgttcata atgcccccca gaggaagccg caaaacatat
      901 ctttttgttc atattaacct ctctatcagt cacgctgaaa aatatctcta gtgtaaactt
      961 tttctttaat atcatccaag caggtatcat atcgattggc aataatagaa tcacacatcg
     1021 ccttaaactg ctctaaatcg ttgataacct tacttccgaa aaaagtctct ccatctttca
     1081 gtgcaggctc ataaatcacc acagtcgcac ctttagcttt tacgcgcttc ataactccct
     1141 ggattgaact ctgacgaaaa ttatcactgt tgctcttcat ggtcagccgg tatataccga
     1201 ttaaaactgg attttccctg ttgacatcat actgattata ttcagcgtag ttgtaatagc
     1261 ccgccatctg aagaacacga tctgcgatga aatccttacg ggtacgatta gattcaacga
     1321 tagcctcaat caaatttcca ggaacatctg catagtttgc caacaactgc ttggtatctt
     1381 ttggcaagca ataacctcca tatccaaagg atggattgtt gtagtgagtt ccaatacgag
     1441 gatcgagaca aacaccattg attatctgct gagtattcag ccccttcatt tcagcataag
     1501 tgtctaattc attgaaatag ctgactcgca gtgctaaata ggtatttgca aacagcttta
     1561 ctgcttcggc ttcagtgaat cccataaaaa gagtatccac gttctcctta attgcacttt
     1621 tctgcagaag tgttgcaaaa acatgagcag catttaacaa gcgctcatca gtcatatcag
     1681 taccaacaat aatacgagat ggatacagat tatcatataa agccttgctc tcgcgcagaa
     1741 actctggact aaaaataatg ttcttactcc ccgttgtctc acggactgat tcagtgtagc
     1801 caacaggaat tgtagacttg atgaccatga tggtatcagg attcacgtcc atcacaacct
     1861 caataactga ctcaacagca gaagtatcga aatgctgagt acgactgtca tagttagtcg
     1921 gtgtagcaat aacaacgaaa tctgcggatt tataagcgct ccttgcatcc aaagtagcag
     1981 ttaagtcaag ttctttttct gtaagatact tttcaatata ttcatcctga attggagact
     2041 tccgtttatt aatcagttca atcttttcag ggataatgtc taccgcggtt acctggtgat
     2101 gctgcgccaa tagtgtggcg atactcaaac caacataacc ggtgccggct actgcgatgt
     2161 tgtacttttt cttcacaata tctcctcttt cctctcttaa gtatggagaa tgttactcct
     2221 acggctattc atcatgtcaa aataacacag cttagaatgt tgataaaata attttccaga
     2281 atgtgataaa atctctttca tttataaacc acacatctaa ttttatctat tctccacaag
     2341 acatgtagag tattct
//
LOCUS       DAFGZE010000172         2355 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.177, whole genome shotgun sequence.
ACCESSION   DAFGZE010000172 DAFGZE010000000
VERSION     DAFGZE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.177"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            31..267
                     /locus_tag="LTU54_003055"
     CDS             31..267
                     /locus_tag="LTU54_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HBL8297687.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            286..501
                     /locus_tag="LTU54_003056"
     CDS             286..501
                     /locus_tag="LTU54_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HBL8297688.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(1204..1956)
                     /locus_tag="LTU54_003057"
     CDS             complement(1204..1956)
                     /locus_tag="LTU54_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBL8297689.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(1971..>2355)
                     /locus_tag="LTU54_003058"
     CDS             complement(1971..>2355)
                     /locus_tag="LTU54_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBL8297690.1"
                     /translation="QANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIE
                     SQDRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEIN
                     KKNPNMFVNYQNDPYQFTTQVILLF"
BASE COUNT          761 a          377 c          398 g          819 t
ORIGIN      
        1 agcaccgtaa aaaatattat ttaaagaagc gtgtttaaac atgcaaaatg taaaggaact
       61 aagtattgtg gaaatgcaca aactgttggt ggaggcaata atgcttgggg aaaattgggg
      121 caagttgtag gtggacttac cacaggggca gtaggtggtg caggtttagg aacagcaatt
      181 tgcggaccag cttgcggtgt tgttggaggg ttatatggag ctgtcgctgg aggtgctgca
      241 gctggctggg atgctcgaaa aaaataaaaa aggagaataa gtagtatgca tattaaaaac
      301 acaaaaacaa catttatatt atcttcagaa gagttgaaaa atattcaagg tgggagtgcc
      361 gtgggagttt taggcactac tttttctggg gcaacagcag gagtaaaact ttgttcagcc
      421 ggaggacctt atgcaatagc tgcttgtggt gtaggtggag ctttattagg tgctggattc
      481 agcatgtgga ccggtgcgta aacaattaaa aaatatcaaa cctcaagatt ggtttgtgtc
      541 gattacgaac ataggtatgt tctttatctt ttcaaaagtt tggaatggac agaacttttt
      601 actaaagctt cttgtgctag tttttataat tatattaaat ttttatcttt tattaaagtc
      661 agataaaaat tctttggaaa tgtaatggta gagatatttt tacaaaagga cttgcagcgc
      721 tgtagttggt gctgcaggag gacctatttc ggccgttaca ggaggtattg gtaatgtact
      781 tgtcggatgt gcattaaagc ctgttcctgc aagttaaatt tataaaaaca ggaaagtaat
      841 tatctcaaat cctatcaagt acaatcatag gattgcactt gacaggacaa attttttaac
      901 taaaaaatga aaaacacttt ctaaaaatta gtagaatttg ccaaatcttt cgcatcttcc
      961 accatacatt taattttaat tcccctttat ggtacttaat aaaaacaaga aaagaactat
     1021 tcatttggcc cttttttggt tccagaccat agaaatattc ctccagtaac taatattgct
     1081 gttatgcacg taactaataa cagctataaa aattggtaca actttaatag gattcagaaa
     1141 aatttgcact agtgaaggaa tctattttat tagaaaaatg caatctccct aaagaaaaaa
     1201 ccactaaaca gtgctttctt tcaacttctc cttaattttt tgtgctttcc ctaaagtaaa
     1261 gtttctggcc aactctgaat caaaatatag tgaacgtttt ttaaaatcta tttctttaac
     1321 gttaaagata tttactaaac acgctcgact aattcttgtt aacataggat atttcttttc
     1381 aagctcacta attcttccat aaaattcgta ctgcccatct ttagtacaca atgataatct
     1441 atgtggtaat tttgaagctt ctacaaaata aatgtcattt atatcaaaag taaatgtttg
     1501 agaacctatg gaaaaaacaa atgcttgatt cgtaacctgt tttgttgcat ctatcctttc
     1561 ttgcgctaat aacaataact caacaatctc gtttcgatac tcatctagtg tttgatcttt
     1621 tgttacaaat cctaacgttt caactcttct ttttattgtt acgggtaaca tctcatcatg
     1681 agtagttgta aaaatgattt ttgcttgaac atcatatttt ctgattactt ctgctaattc
     1741 tataccgtta acttcatgat ttaaatcaat atctaagaaa tatattcctt gctttggtcg
     1801 gaattgtttg aggtattttt taacttctaa cggactttgt gttttcaata ctatcttaaa
     1861 tactttatcg tgaaaaagaa tgaaattatc aataattcgt tctaactgat ttaattgtat
     1921 aatttgatct tcacataata ttattggata actcataaat cctccttccc tcaaaacaac
     1981 aaaataactt gagtagtaaa ttgatacgga tcgttttgat agttcacaaa catattggga
     2041 ttctttttat tgatttcctc tatactagat aaacctaacc cttgatgtcc cttttttgtt
     2101 gttattcctt ttttcattag agtatctata gagagtagta tctctctaca actattcgat
     2161 atcgaaattt ctatctgttt atcatctcgg taaatagcta gagacagttc tctatcttga
     2221 ctctctatag atgcttcaat tgcattatcg agaataattc caagcaccct aatacagtca
     2281 aatattggaa caggaacttc aataatagta cttggacatt cgaatttaca acaaatctta
     2341 ttgttatttg cttgg
//
LOCUS       DAFGZE010000173         2351 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.178, whole genome shotgun sequence.
ACCESSION   DAFGZE010000173 DAFGZE010000000
VERSION     DAFGZE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.178"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..544
                     /locus_tag="LTU54_003059"
     CDS             <1..544
                     /locus_tag="LTU54_003059"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8297691.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="LTU54_003060"
     CDS             complement(1017..1589)
                     /locus_tag="LTU54_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297692.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="LTU54_003061"
     CDS             complement(1605..2210)
                     /locus_tag="LTU54_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HBL8297693.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          359 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta g
//
LOCUS       DAFGZE010000174         2256 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.179, whole genome shotgun sequence.
ACCESSION   DAFGZE010000174 DAFGZE010000000
VERSION     DAFGZE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.179"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(572..937)
                     /locus_tag="LTU54_003062"
     CDS             complement(572..937)
                     /locus_tag="LTU54_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297694.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1013..1258)
                     /locus_tag="LTU54_003063"
     CDS             complement(1013..1258)
                     /locus_tag="LTU54_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297695.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1328..1582)
                     /locus_tag="LTU54_003064"
     CDS             complement(1328..1582)
                     /locus_tag="LTU54_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297696.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1746..2069)
                     /locus_tag="LTU54_003065"
     CDS             complement(1746..2069)
                     /locus_tag="LTU54_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297697.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
BASE COUNT          854 a          371 c          390 g          641 t
ORIGIN      
        1 agaaattatt aattttcgtc tgaaaacaaa aaaagaaagc gttattattt tagtctattt
       61 tcaggggggg aaccttatga taaaaaaact atttttacta tcaatattaa cgctagcaat
      121 ggcaggttgt tctgactctg gcttagcaac tgaactgatc acaaaatcaa ctgaagccac
      181 gacagaggag ttaaaatgtg gaaacgatca cagtgactac agaggcttaa atcgacgatg
      241 catgggaata ttaggcagaa gcaattgtaa gcatcgatgt agtaatcgaa gaaggtgtag
      301 aagaagcaac agaaaaatca aaagaaagat caaaagaagc aatctgtgct gtgcatcgta
      361 tacagacgtc gaagatgcgg tacttttaaa ccacgtcagg agatgttcat ttatgtctag
      421 ggaagttggc gatgaaccaa aactagagta cgcttttagt tttgcacgtg aggatgaaga
      481 taaccctaaa acttacgaca ttttagcttg atctaagatg gttaaagctt tttaatgaag
      541 taagaggaga tagaaggctt ttctagttat attaggtaag ataaattcct ttttgctcca
      601 aatttgctaa acattctgct agataaactt catcgtgttc cggataaatc ggcacgttgc
      661 gctctacagt aggtaaatca aaaaatgctt cgcatgattt gccacgatac ataggatttt
      721 tccactctgg tgctgggctg taaccacgtt tttccatttc atccatgaca agctgatgat
      781 attggaataa tctataagga gaatagtgaa aaacataatc cacagtagca tgtttctttc
      841 cccagccatt tcctcttaat gcacagcatt cccgatgttg tcctaaaagc tgttgacgag
      901 gaagctttga aatcaatttt tcatgccata atcgcataga aatgtctcct ttatatatag
      961 attgttaacc ttagacgtta aatgaaaaaa ccagcaccaa ggctggaaaa aatcacttca
     1021 gtttgcttgc tgtttctgag cgaccaatta ggtaatcaac ggaaatatcg taaaagtcag
     1081 cgatatagat taattcaatg atgcttggaa tttcttctcc ttcttcgtac ttacgatatt
     1141 tgaataaagg aatacttaac gcgctagaaa cagctacttg agataaagat cggccagttc
     1201 ttaagaaatt cagacgacaa cgtaaagtat ccagtaaaat agcttcttcg atactcattt
     1261 aatcaccttt cattatagta gaatagttat aatgactata gaaagctaca catgcaatca
     1321 atagtactca atgtttttta tttaaacgta tcaaataaag aatgacgatc aataaagaca
     1381 aaaccagtcc tgataattct tctataaaaa cactatcggg ttcaaagtag ttgtaattaa
     1441 aaactttcca cataaagtag atagccggaa gagtcaaaat agctaaataa aaccgttctt
     1501 tgttccaatt cataagataa acaatacttg cgaccagtgc gaccgcaggc acccaaaaca
     1561 aaactctcac caacgtaatc atgtaacgga ctcctcaacg attcatataa taaaagataa
     1621 catgttattg catacatcac aaatgaaatg ctaggaaaat aaaagaagaa tagatcaatg
     1681 aaaaatgaaa aagcagaaca gttttctgct gataagcagg aactgttccg ctgaatgtgt
     1741 cttatctatc gagaaaaaat caatggattg atatctaagt aattcaatac atataaaaag
     1801 aggacgatcg cattcactaa aaaggaaaga atgatgatcc catagcgaat ggaaacatat
     1861 ttaacaaata cttttgtttg aatacgggca tatctttttc ctttatagct gagcatcatg
     1921 acaagaagca aaagggtaat cacaccttga aaaatcattc caataaacag aattcctgag
     1981 gtttgataat ttaattggag aaaataagct gctgccgcaa ctaaatcgaa taaaatcgca
     2041 ataaatggca aaataatcac tcctaacatt gagaataaat aaaagaataa cattttttct
     2101 aaaataagca aataaaaatt aaagtaacaa aatagttatg acacaagtaa aagaaataag
     2161 aataacaata ttattttact tatattttga aacttgtgag agtttgtcat tcgtgctata
     2221 gttgaaaagt aacaaaaata aattttattt atgatg
//
LOCUS       DAFGZE010000175         2219 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.180, whole genome shotgun sequence.
ACCESSION   DAFGZE010000175 DAFGZE010000000
VERSION     DAFGZE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.180"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            51..758
                     /locus_tag="LTU54_003066"
     CDS             51..758
                     /locus_tag="LTU54_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297698.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            1139..1318
                     /locus_tag="LTU54_003067"
     CDS             1139..1318
                     /locus_tag="LTU54_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297699.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            1449..1718
                     /locus_tag="LTU54_003068"
     CDS             1449..1718
                     /locus_tag="LTU54_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL8297700.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            1711..1968
                     /locus_tag="LTU54_003069"
     CDS             1711..1968
                     /locus_tag="LTU54_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL8297701.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
BASE COUNT          818 a          304 c          458 g          639 t
ORIGIN      
        1 tatcgaaaaa aatggtgcat tttatacgat tgaatcggag gagaaagata atgaataagg
       61 tggtttatca gctaacaaca gaagatgttt cgcaattttt aacaaacaaa gaacacttga
      121 atgaagtaat cagacggatt caaaaagact ttagttgtat ggaagaaatg aagtacttta
      181 ttcaaagagt ggaagaacag ttgtatatgg aaagtgaaac gagtcaaacg aaggttacag
      241 tcagccataa tcgtttagac gtgtataaac gtcgtcctga cacattaaaa aaaggagcgg
      301 agcttctacg actagaaaca gaaaaatata tcatgaatgt ttacctagaa aggagtgagc
      361 tggtcgaaaa ctttgtcagc tacacgattt gcaggaaaca ctattactca gaatcagaat
      421 attattcaat ttttgagagc atggaattaa tggaagaaga aatggaagag ctaaacagca
      481 aagatgcgac ggtagacgat tattaccttg tatttactag atttttgacg tgtgggacgg
      541 aatctatcgt gttaatggga atggattatg aactcactgt acccgaagca tttttgaggg
      601 aatatgtaga agaagtagat aacccaggcg acgactggta cacgacttta actgattttt
      661 tagacaccta cacttacgac gatacagaag aaatccaaaa tgaacttgac cgtaaacgaa
      721 tgccatatca acgatttgaa aggaaaaaag gtcaataacc atttataccg aaaagtgagg
      781 atgtgtgaaa taagaacaaa aaaccttgaa attaagggaa tggttcttat ttcgcaccaa
      841 tcgatccaac cgcccccgcc gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc
      901 taagagaaag aaggagatcc atatgaataa aatcattcct ctattgttcg tactgtttgt
      961 tggcgagata cttatgcgtc atggtgcagc tctacattta aacttcgtag tattcttagg
     1021 aatactggta ttttgtttga tcttttggcg ctttgtgtta acccttgtga gtctaatatt
     1081 caagttgatt gcagccatat ttttaatagg gttatttcga tgaaaggaga gagtactaat
     1141 gccaattgca ataggaaata aacggttacc cgtgacgttg gatgaaaaga gacaaaaaga
     1201 attgcagcaa ctaaagcaga agtacggcaa aagtgaatcc aggattatgt gtattgcgtt
     1261 agatttattg attgcccaag aaaaagcagg atttgaggta ccagcactca aaaagtgacg
     1321 tcacttttta tcctaaaaac taaaagtgat agcactttta attataagaa gttagaatat
     1381 taatcatttg cttaattgta caatataatg tacaattgtt ttatagaaat aaataagggg
     1441 tgaaaggaat ggaagcagta gcttattcaa atttccgcca aaatttacgt agttatatga
     1501 aacaagttaa tgaggatgct gaaacactta ttgtaacaag taaagatgta gaagatacag
     1561 ttgttgtatt atcaaaaaga gattatgatt ctatgcaaga aacgttgaga acactttcta
     1621 ataattacgt catggaaaaa attcgtcgag gagatgaaca attctccaaa ggtgcattta
     1681 aaacacatga cttaatcgag gttgaatctg atgattaagg cttggtctga tgatgcttgg
     1741 gatgattatc tttattggca tgagcaagga aacaaaagca atataaaaaa gattaacaag
     1801 ttaataaaag atatcgatcg ttcccccttt gctggattag gaaaacctga gccattaaag
     1861 catgatttat ctggaaaatg gtccagaaga attacagatg aacatagact gatatataga
     1921 gttgaaaatg aaacgatatt tatttattct gcaaaagatc actattaacc aatcggaagt
     1981 aaggaaaggg tcagaaactt aaaagttttt gatccttatt ttatttaccc tagtcattta
     2041 aaaagctaat atagcttagt gttgattgtt attaatgaat gtgtttgtta cgcgtattac
     2101 ggatataagg ttagtaaaat catttctaaa gttgaggaaa agtaaatata aatggcttaa
     2161 atttcaacaa tttgaagttg aatagatatg ttataatact attgtagtgt gggatgtta
//
LOCUS       DAFGZE010000176         2206 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.181, whole genome shotgun sequence.
ACCESSION   DAFGZE010000176 DAFGZE010000000
VERSION     DAFGZE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.181"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..625
                     /locus_tag="LTU54_003070"
     CDS             <1..625
                     /locus_tag="LTU54_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297702.1"
                     /translation="ARMHKFSELDTATKELQAEMTSYIVCKHFGMDTLEKAIPYIAAW
                     TKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKN
                     TVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYG
                     KEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            686..2155
                     /locus_tag="LTU54_003071"
     CDS             686..2155
                     /locus_tag="LTU54_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL8297703.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT          841 a          343 c          477 g          545 t
ORIGIN      
        1 tgcaagaatg cacaaatttt cagaattaga taccgcaact aaagaattgc aggcagaaat
       61 gacttcttat attgtgtgca aacattttgg aatggacacg ttggagaaag ctattcctta
      121 tattgcagca tggacgaaga atggtcagac gttggatgat aaggaagctg cggaacgtgg
      181 gaaaatcatg aatgatgtca gccgtgtcgc aaatgaattc attcaaacca tttctaatga
      241 aatcaatcaa tacagagaaa gagaaccaga gatccaacca gcagaagtga agcaggagca
      301 taaaaatact gtagaaacgg cagaacaaaa gaatattgtt cctgttggtt cactttggat
      361 cgacaagaaa gacgggaaag taatggaaga agatacgggt cgaacgttac atttgaagga
      421 tgcggcgttt attgaagatc cacagcataa tcaagtaatt ttaaaaggaa aaagttatgg
      481 aaaagagatc caaacggcgt taccttcaga agaatttgcg ctaggaaatt cattccgatt
      541 ttcacttgag acagtgtcag aagtggcaaa acgaaaaaat gaaatctatt atgaacaaca
      601 attgtcacaa aaagaacgtg tataaaacaa gtaaaaaaga ctatctgaaa aacggatagt
      661 cttttttctt ttgggaggtt ttaaaatgac cgagaaaaaa atgagtctta ttgatcgatg
      721 taaacagatc gatatagtgg attttgctcg aaacaatgga atggctgtag tcaataaagg
      781 acgagactat cgcttagaag accatgattc atttgttttt gatcgaagaa aacaacgttt
      841 ttattggaac agtcaaaata ttagtggcga cattattgaa ttagctaaac tttttttcat
      901 agacaaagag atccaagacc ctaagcaaca atttaaagca gccactgatt ttattctaaa
      961 gaatgaagac aaaacagaac gagtggaaaa tcttcatttt gagacagaaa agtacaaaga
     1021 tcaccccgtt gattatcaac cactcacaga aaaaggaagg aattatctga aggaagaacg
     1081 aaaactacca gagtggctga ttgattatgc agaaaaagaa gggttgattg ctgaactaaa
     1141 gccgaaacat gaacggcaaa attttttagt tggagacgac cgtttagatc atgcggttgc
     1201 ttttttatgg aaagatccac agacaggaga gacagtaggg gcaagttatc aaggaacgat
     1261 cgtcgatttt aatcgtttcg gtaaacgggg aacgtataaa cacattgaca agaatcctac
     1321 gccaaatcac ggatttaatt taaaaatagg cgatccgaag catttaaaat tctttgaaag
     1381 tagtattgat ctgttaagtt acgctgcatt gaatcgggag aaactacaag atgcctggtt
     1441 agtttcgatg gacgggttaa aacatcatgt aatcagtcat tacgttgaag agtcgatttc
     1501 tgaattaaga cgaaaacaaa catttcctca atcgattgaa atatgtgtgg acaatgatcg
     1561 tgcaggacat attttttatg aaaaagaaca gatgaaggga atcgttgatc cttttaccaa
     1621 taagaaaatc cgttgtgagc gaggaattcc aaatgattgg caagtaccta aagaatacaa
     1681 agctacttat gaagcagtcg caaaagaaat gagtgttgaa cctgaagcaa ttatggccat
     1741 acacaaaact gaaactaatt tacagctgac aaatcaattg gtgtctgctc atgatgttca
     1801 atctacgttt ggaaagatgt tggcgaaggg cgaaccagta gaaacaattg atttgaaaga
     1861 agcatgtacc acggtcgcga aggaactgaa agtatgtgaa cgcgcagatg gaacatacaa
     1921 ctttgatcgt ttttatagta gaaaagccaa cataaaagac gttaatgcag gaatccttct
     1981 ttcctataaa gcagaacaat actacaaagg ctataaaaag catgaacatg aatttgtacc
     2041 tgaagtgaaa aaggattgga atgatcaatt gaagcatgag atccagcaac aagaaatcag
     2101 aaagcaaaaa cgtgcgatgt tgtttcaaca aggcagacaa caagaaaggg agtgaagtcg
     2161 tcactatgaa aaccgtcatt ttagcagaaa aaccgtcaca agcgaa
//
LOCUS       DAFGZE010000177         2160 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.182, whole genome shotgun sequence.
ACCESSION   DAFGZE010000177 DAFGZE010000000
VERSION     DAFGZE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.182"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..424)
                     /locus_tag="LTU54_003072"
     CDS             complement(<1..424)
                     /locus_tag="LTU54_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HBL8297704.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..1564)
                     /locus_tag="LTU54_003073"
     CDS             complement(533..1564)
                     /locus_tag="LTU54_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8297705.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          679 a          344 c          365 g          772 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 aaaatcgtaa aaaatgattt aatgaatatt gcttccttga aatcccgtaa tagctatttt
     1261 caaacaattg ctccgtttct tggttcgctg tagtttggaa cgaacgagta tgtcgataga
     1321 ttttagctaa gaaattctga taatcttgtt tttctaaatg gataatcagc tgatcatcca
     1381 cttcttcaga aaatactttt ctcaaataat cagtaaacag atgaaaatct tttgcgtatt
     1441 catagcgact gcctaatgaa agggaagaac gattttcttc taaaaactgt tgaaagacgg
     1501 aagggagcaa gtgtgtacgt tgctttaact tttcttgcag ttgttttcta gttttgctgt
     1561 ccattacaaa ttccctccaa tagtgttcat tttctatatt ttaccgcatt tcatcaaatg
     1621 aagatagaca gtttgtcttg atcttaggaa gcaaattttt aactaagtac tagctaaaag
     1681 atatgaagta agaagataca tttttagtta ttttattagt gcagtcattc ttttgatctt
     1741 ttaattgatt tatcctcttt ttagctatgt ggttacttca tgagcaatga aatggatttg
     1801 gttcgttcta atggcaggag gctctttctt ttatttccga aaaattagaa aacatttttt
     1861 cagaatcgat tggctctcca tttattcaat aatggtttgt ctaacgatat tgttgggata
     1921 attcttaaag aagtattaga aatagttggt acttcattac tttacatgaa gatttattct
     1981 atggtaggag caattatatt gagaattaat ccttttttat ataagttgtt tgcttattga
     2041 tggattggat aaattaaacc atcacaacga gtggaaatta tatgaaattt ttcataaaaa
     2101 ttcatataga aacaaatgac attttattag aatatgactt tcattaacat aaatgttttc
//
LOCUS       DAFGZE010000178         2083 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.183, whole genome shotgun sequence.
ACCESSION   DAFGZE010000178 DAFGZE010000000
VERSION     DAFGZE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.183"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(856..1386)
                     /locus_tag="LTU54_003074"
     CDS             complement(856..1386)
                     /locus_tag="LTU54_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferase"
                     /protein_id="HBL8297706.1"
                     /translation="MRLGLIIKNILNLPKTIYFNYKFLHISDAWRLPILVHRKVRITR
                     KSRGKIEIVLQSGKIVYLGFPVLNTAGNEETIISIEGILRIQGPAFIAQGAHIDIRKN
                     AVLDIGSKFNSTGKMSIICFKEIQFKDGCTVSWDTLFMDTDSHTIFELNTHRILNKNN
                     KIVIGKHCWIGAKSVI"
     gene            complement(1383..2000)
                     /locus_tag="LTU54_003075"
     CDS             complement(1383..2000)
                     /locus_tag="LTU54_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBL8297707.1"
                     /translation="MIIVIAEIITDILYKLYEPFGFAVLLTILGLVYFMYATDGIAAG
                     EGYKRILMELYLHLKKDRKYRKVCALIFYTAMILFTTLINRTIWLNPLSDVMGDWWIY
                     KYNTTTGTKELSTACLENIILFIPYVYMVMSIKYENIKKPKIWSSIVYGGKIAFYFSA
                     IIEFLQLFLRLGTFQISDFVYNTLGGILGVILYKLILITKRDGER"
BASE COUNT          676 a          405 c          231 g          771 t
ORIGIN      
        1 aatttaaaat taacattgat tctcctatga aaatatcttt tacctttctc ctgtgaaaag
       61 gaaatttcct tttttatagt gttattgata taatcagaat attcatcaaa ctctgtatct
      121 ggagttaaaa atctacagta acatctataa gctttgctag taatcttctt gggttctctc
      181 tccattgtca agcaacatca aaacacctac ttactattcg attcttttct gccaaaaacg
      241 agtcagcatc actcacaact tatgtgttgc tgactccgac tagtcctttg ttcaatatca
      301 acttattaaa cagatataat atttttcttt atttcgtata ctccaagact tccatctttc
      361 tttcatctta tctctgaaaa aaacacagca aagtgccctt aataaatata cattagaatt
      421 tgcaaatttt ttcatcttaa gatatatcct attcatattc tttacaatcc taatgggtga
      481 ttacaaatct atttagttca agttctacat tataaacacc tttcttgata gtatcatctg
      541 ctgcacaagc gatgtcgatc cacttattag gggatttacg tgctcaaaag tggctattcc
      601 ttttgggaag tccatccata ataatggtcc atggattttt ttagggtctt gtcgttcaaa
      661 tttctcaaaa actttattat acttccatag ttttataatt atttggtttc actttggctc
      721 gttcaaacac caacttgtat ttcttttcaa cgttcgaact ggagcccccc ctataatctc
      781 attattattt tcatgacttt ttactaccgt ggaattacaa gcaacaacaa tattatctgc
      841 taaatgaaca cctttctata tcacgctttt tgctccaatc caacaatgtt tacctataac
      901 tattttatta tttttattca aaatcctatg agtattcaat tcaaaaatcg tatgtgaatc
      961 ggtatccata aataatgtat cccacgatac tgtacagcca tctttgaatt gaatttcctt
     1021 aaagcaaatt atactcattt ttcctgtcga attaaacttt gacccaatat ctaaaactgc
     1081 atttttcctt atgtcaatat gagccccttg tgcgataaac gctggccctt gtattcttag
     1141 tattccttca atacttataa tcgtttcttc atttcccgca gtatttaata ctggaaagcc
     1201 caaataaact atttttccag attgtaatac tatctcaatt ttccctctcg atttgcgtgt
     1261 aatccgtact ttacgatgca ccaaaatagg taatctccac gcatctgaaa tatgaagaaa
     1321 tttataattg aagtatatcg ttttaggtaa atttaatata ttctttatta tcaatccgag
     1381 tctcatcttt caccatctct ttttgtaatc aaaattagtt tataaaggat tacacccaat
     1441 ataccgccca acgtattata tacaaaatcc gaaatttgaa acgttcctag cctcaaaaaa
     1501 agttgtaaaa attcaattat tgctgaaaaa taaaatgcaa tttttcctcc atatactatt
     1561 gaggaccata ttttgggttt ctttatattt tcatatttaa tagacattac catataaaca
     1621 tatggaataa aaagaataat attttctaaa cacgctgtag ataactcttt tgttccagtt
     1681 gttgtattat acttataaat ccaccagtct cccataacat cagataaagg atttaaccaa
     1741 attgttcgat tgattaatgt tgtaaataaa atcatcgcag tatagaatat caaagcacaa
     1801 acttttctat attttctatc cttctttaaa tgaagataaa gttccattag tatacgttta
     1861 tagccctcac ctgcagcaat gccatctgtt gcatacataa aatagaccag tcctaaaatt
     1921 gtcaataaaa cagcaaaccc aaagggttca tataatttat ataatatatc tgtaataatt
     1981 tctgcaatca ctattatcat aattatttct ccaatatcaa gtatacactt atcgcttcca
     2041 attacacatg atcatattag agtgtattat cacattctct aat
//
LOCUS       DAFGZE010000179         2020 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.184, whole genome shotgun sequence.
ACCESSION   DAFGZE010000179 DAFGZE010000000
VERSION     DAFGZE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.184"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            100..570
                     /locus_tag="LTU54_003076"
     CDS             100..570
                     /locus_tag="LTU54_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HBL8297708.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            792..1895
                     /locus_tag="LTU54_003077"
     CDS             792..1895
                     /locus_tag="LTU54_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBL8297709.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          686 a          328 c          460 g          546 t
ORIGIN      
        1 gaatctctga agaataaatc acgtgatcgt gacgataaaa tacacttacg tcaggtgata
       61 aaaaatacat aagtagaaaa ctaagaggag gaacagaaaa tgaaagaagt tcaattgatc
      121 agaaaaagcg aactatcaga aggtggttgc aatgcatgcg gcgtagtaga agcaacaagc
      181 tatacattaa agcttggtgc caacaaagcg ataatctctg agttgacagt aggcggtctt
      241 gtggacagtc tggcgcttgc tgaaggtttt attggagaag atatttatga gatgttttca
      301 gaaatccgtc agttaaaaaa aggggaaaac tgcatagagg ttcatcatga gagcccgaat
      361 gtgagattca aacgtggtga taatgagatg atcttcaata atcatgtgtc cgatcacacc
      421 gagctatatg gaatcgtgaa tcaaatacta actgagcttt ttggattggg accttatgcg
      481 ttcaaggaag aaaacggtaa tccaaaatta aacgaagaat ggcaagaaac gattgaaata
      541 caaagaaaca acccgcactt atttcaatag gcatccacag tatttcgtaa aagggacttt
      601 gacacgactt ttgtcaaaat cccatcgttt tttatctatt tgtgttctaa acatttcttt
      661 cagagtagta acaactggaa tgactgaaat gtaaacagat tatttgcctg tatcaatacg
      721 gaagagtaat ataaagtgtg taaacgctat catattattc attcaatttt ttatagaggg
      781 aaggcattga aatggaaaaa acgtatcaag caggtacaaa tgaaggaata gtggatttta
      841 tcaatatgga agatttagaa atagccgctt cacaagtgat tccagctggt ggttatggtt
      901 acatcagcag tggtgctggc gacttattta cttatcaaga aaatgaacgg gcattcaatc
      961 atcagctgat tattcctcat gtattaagag atgtagaatt acccgataca actacacatt
     1021 ttgacaaaga aacgctgact gcaccaatca tcatggcacc agttgctgct catggcttag
     1081 ctcatgtgaa ggcggagaaa gcttctgcaa aaggtgtagc agattttggg acgatctata
     1141 cagcaagttc ttacgcttct tgtacattag aagagataag agaagcaggc ggggagaaag
     1201 caccgcaatg gtttcaattt tatatgagca aagatgatgg gatcaatttg gatattcttg
     1261 aagtggccaa gcgtaatgga gcaaaagcaa tcgtattgac agcggatgca acagttggcg
     1321 gaaaccgtga aacagaccgc agaaatgggt ttaccttccc actgccaatg ccgatcgttc
     1381 aggcttatca atcaggagta ggccaaacga tggatgctgt gtacaaatct tccaaacaga
     1441 agcttagtcc aaaagatgta gaatttattg ctgctcattc tgatttgcca gtctatgtga
     1501 aaggtgtcca gtcagaagaa gatgtctatc gttcattaga atctggtgct ggcggaatct
     1561 gggtatctaa tcatggtggc cgtcaactag atggtggtcc agcagcgttt gattcgttgc
     1621 aatatgtggc agaagcagta gataaacgtg taccaatcgt gtttgacagc ggtgtacgac
     1681 gcggacagca tgttttcaaa gcaatcgcat caggtgcaga tcttgttgct attggcagac
     1741 ccgttattta tggattgtct ttaggaggaa gtacaggagt ccatcaagtg tttgatttct
     1801 ttaaaacaga attggaaatg gtcatgcaat tagctggaac acaaacagta gaagatatca
     1861 aaaaaataaa attgcgtgag aatcgtttta tttaagaaac aaatgacgaa aaaaatagga
     1921 gtgcggcaga tgatgccgta ctcctatttt tatagttcaa ggatgtcact aatagaataa
     1981 tttgaaacga aaaaataaaa ttgaaatatt ttttcatact
//
LOCUS       DAFGZE010000180         2008 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.185, whole genome shotgun sequence.
ACCESSION   DAFGZE010000180 DAFGZE010000000
VERSION     DAFGZE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.185"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            385..1002
                     /locus_tag="LTU54_003078"
     CDS             385..1002
                     /locus_tag="LTU54_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297710.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1043..1342
                     /locus_tag="LTU54_003079"
     CDS             1043..1342
                     /locus_tag="LTU54_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HBL8297711.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1376..1543
                     /locus_tag="LTU54_003080"
     CDS             1376..1543
                     /locus_tag="LTU54_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBL8297712.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1588..>2008)
                     /locus_tag="LTU54_003081"
     CDS             complement(1588..>2008)
                     /locus_tag="LTU54_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297713.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT          681 a          313 c          388 g          626 t
ORIGIN      
        1 gtatcaaaaa gatttatttt taaccctaaa ttagaaatat atagtgttgt aatagaataa
       61 tagtgttaaa aaaattgtaa aagttaaaga agtcaaattc tgatgtgaaa aataaaatat
      121 accatggaag acataaaaga aaaactggat aactagttta tttaatggca gaccgttaag
      181 gcctgccatt tttatgtatt ttagcggaat aaatataatc atgcgggagt gtaaacttac
      241 tgtttcaaaa agtatacttt ttgacatgcc ttgattttct ttgtttatgt atggcataat
      301 aaccacataa tacaatttga aacgtttctg atttaatctg attgttttga aacaatttta
      361 gtgaaaaaag ggtagataaa aacgatgaga aaattttttt atatccgggt cagtagttat
      421 gatcaatcga tcgaacgaca actgatttcc gcgaaggaat taggaatccc tgaagaatat
      481 atatttgtag aaaaagccag cggcaaagat tttaaacgcc ctgaatatca attaatgaaa
      541 cggatgtttc gaaaaggaga tttactctat ctacaatcat tagatagact aggcagaaat
      601 aaacaaatga ttttagaaga atggcgagaa ttaactaagg aaaagcagat tgatatttct
      661 gtgttagaca tgcctctgtt agatactgct agttacaagc aactagatgg gttagagacg
      721 cttatctctg acttaattct gcaattactc agctatatgg ccgaagacga gcggaaacga
      781 attaatagcc ggcaaagaga aggcgtagaa gctgcgaaac aaaagggtgt aaaattcggc
      841 agaaagaaaa ttgctttacc tcctgaattt cctgcgatct attctgactg gaaagcagga
      901 aagataactg cagtctatgc tatggatcaa ttgggcatga aacgaaatac cttttatcga
      961 cgggtgaaag aatatgaaca taccttgcac cacttttctt gatagatata acagctatta
     1021 gacgggaagg gagagtttga aaatggactt aacgaatcaa agggtcgttt ttactggaac
     1081 gctgcagcaa atgacacgaa cccaagccat cggtttggtt cactctttaa acggccattg
     1141 tcaatcaagt gttacttcaa aaacaaattt cctggtttgt ggtcagtatt ctaagtcgtt
     1201 gtttgatgat tctctctata caaaaaagga acaaaccgcg cgggacttga ttgcgttagg
     1261 tcatgagatt accattttat cagaagtcga attttatgcg ttaattagtc aacagttgaa
     1321 agaatggcaa cggtatttat aatcgttagt aaaatttaca aggaggaaaa tttttatgaa
     1381 gcgaaaatct tatgatcgtt ggggcattga aataactagt tttaatcgaa atattgcttt
     1441 tcacgtggag tgtgactgtg gggaattagc aaaaagtgtt tatcaaaaaa attactttta
     1501 ctgtcctaaa tgccatcgcc gatacggggt acatggaaca tagaaacgaa atagtgcggt
     1561 agaaaagcga atgaatgtag tatatttcta caaatcattc gctttcttta ttttataaat
     1621 agttggtcga gagatcccgt tttcttttgc aatctctgca actggaatgc cttgctttaa
     1681 catgtccacc acggttttaa aaactaagcg tttttgcgga tctgttgagt taatagaata
     1741 ctcgattggt cgtcccttat atcggccggc tttttgagcc aataatactc cttgccgctg
     1801 acgttcacga attttctcac gctcgttttg tgcaatataa cttagcagtg ataaaaacat
     1861 atcgaacata gcggtatcca ggagttcatt tccagtatta aaatctaaaa actgtgcatc
     1921 taatattttt aacgctactt ttttctgttt cataaaagca actgtatttt ttatatcttc
     1981 ataatctctt cctaaacgat ctagtgat
//
LOCUS       DAFGZE010000181         2005 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.186, whole genome shotgun sequence.
ACCESSION   DAFGZE010000181 DAFGZE010000000
VERSION     DAFGZE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.186"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(44..1477)
                     /locus_tag="LTU54_003082"
     CDS             complement(44..1477)
                     /locus_tag="LTU54_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBL8297714.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKGELI"
     gene            complement(1470..>2005)
                     /locus_tag="LTU54_003083"
     CDS             complement(1470..>2005)
                     /locus_tag="LTU54_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBL8297715.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDK
                     LLNRIDKEILKVGNIDE"
BASE COUNT          757 a          325 c          239 g          684 t
ORIGIN      
        1 atattaatca aatgtaaagg agtccaagca atgaaagctg tcttcatatt aattctcctt
       61 tttttaaata caagtaagaa atgcttccca aataatacca gaaaacaatt gaaatgacat
      121 cgcgcttatt gaaaagtaca tgagcctcca ccatttcatc tataaaaata gatagtataa
      181 aaattaaaac aataccaaga aacgcataat tcactttact tctaaaaaat gttttgagta
      241 aactaaagat atttaacaca tagaaagcat agatacatat aaaagcaatt ataccacttg
      301 atacaagtac ttgaagaaat ccattatgca tgtttccatg tgctcttttt agttcattca
      361 catctgtagt atcaagatct acttttttca aaaattttgc atcgctattc ccatagaaat
      421 cggtctctcc ataaccaaat aacaaagttg gtcttataac tttaagcgca gctttccaaa
      481 tcgtaaaacg accagctgaa atttcggagt tatcactaga atgctgtaat attacttctt
      541 tttctggaag tggagtttta tctgattcat ttgactctat aacttctact tttctatcaa
      601 tcgatacagg aattgttgct aaaaaatcct tagcataatt agtaccaaaa gaaaaatata
      661 ccaatagaac taataatatg cttaccatcc atttgatttt tacaggtagt acttctctaa
      721 tgctctttct aaattttgaa ttggcaaaca acaaaagaaa taatactaaa aaagaacaaa
      781 ttactacctg ggcacctcta gacgaagaaa gtgtaatata aaatatttgt acgataaaat
      841 tcatcatata aaaaacacga atcgtttttg ttatttttga aattaataca ctaattaaac
      901 ttaaaataat agaagtatat ccaaacatag ctccaacatt tggacttgta tagacaccaa
      961 ataaccgatg ttcaataaaa ccttgtcttg caagtaatcc tggcgttcct gttgggacat
     1021 gataagaaat caaaaaaata aattgataga tagatattag cgcagctaaa aaaattatga
     1081 taatgaaaac attattaaaa tacaccattt ttttcttttt ttcctcttca ctttgtgaaa
     1141 agtctgcagg aaaaattact atcaaagtaa tcagcaaata tccaaaatta taaatagatg
     1201 gaattaaaaa attttctctg ttaatgatta tagttaagat aaaactaatc attgctccaa
     1261 tcagtagcca agaaaaacgc attttaaaaa aatttttctt tataaaaacc tcatacaaaa
     1321 ctataattcc accccaagca aaagctattt tcatcgggat attagttaca ttaaaagtac
     1381 taggcataaa ataaagcaga caaatgatta aatttattac tcgaaacgca tctcgactta
     1441 ataaactctt ctctaaaaca ggtactattc tattcatcta tatttccaac tttcaaaatt
     1501 tctttatcaa ttctatttaa tagtttatca gagtaatccg atttaacttt tcttacaaat
     1561 aatttttcac tttcttttat ttgatctata tccctttcat caaaccattt gttcatggaa
     1621 ggatcaataa tatgaatatt tgctaatttc gaagtttgta aagtagtttc ttcaatattt
     1681 ttcaattcaa gtggtaaatc tggtcctcca ataacatttt tatttttgaa cgaggaatta
     1741 aaaaaaagtg tatgaaaaaa tttttcatct ggggcaaaca tacctatttt aaaattcagg
     1801 tagtcttgtt gattttgttc atacatttgt attaattctg ttaggcattc tttcgacaaa
     1861 gcccaccatt gagaaccatg atacaaatca aatttatggt gtctaatttt tgggggaagt
     1921 ttaatcatcc tcagacaacg attaattata gctctaaaaa ttttaaatgc agttgtgtta
     1981 ttcctattaa ttctaggata atcat
//
LOCUS       DAFGZE010000182         1994 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.187, whole genome shotgun sequence.
ACCESSION   DAFGZE010000182 DAFGZE010000000
VERSION     DAFGZE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.187"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            74..403
                     /locus_tag="LTU54_003084"
     CDS             74..403
                     /locus_tag="LTU54_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL8297716.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            393..680
                     /locus_tag="LTU54_003085"
     CDS             393..680
                     /locus_tag="LTU54_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL8297717.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            965..1822
                     /locus_tag="LTU54_003086"
     CDS             965..1822
                     /locus_tag="LTU54_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBL8297718.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1823..>1994
                     /locus_tag="LTU54_003087"
     CDS             1823..>1994
                     /locus_tag="LTU54_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297719.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQ"
BASE COUNT          741 a          266 c          393 g          594 t
ORIGIN      
        1 cttgtaaata cattatctcg tggtataatg tatttactaa gtaattacat actgttagaa
       61 atgagggatt taaatgatta caaaaacaag aaaacaaggc aattcaatca tgttgacagt
      121 acctaaagat tttaatgttc ctaatggagt cgaagttgaa gcaaagttgg tagagaatgg
      181 tattctttat gaatttgttg aaccacaaaa agaattttat gattttagcg aagatatttt
      241 gtctgatata attgcagaag gctacgataa agatgagatt cttgtagaat ttaagaatag
      301 aaaaaacaaa atgcattctt cctttagaga tattgctgaa gatacactta ctaattctaa
      361 agtaatgact aaagaggagt tggcaacaga aattggctta tgaaatcgtt ccttcaaaac
      421 atgtcattaa gtatttaaaa aagctaaaag agaagccatt gaaagaaaaa tttttgaatt
      481 taatttatga tgaaattgcg attgatccat ataagggtga acaaaaaact ggggatttat
      541 caggtatttg gtctagtggg ttcaaatatg cgggcacaac atatagagta gcttacgaga
      601 ttatagaaaa caaggttatt ccagtattgt tatgtggaac gcatgaaaac ttttatgaac
      661 agttgaaaaa attaatctag tgatttgttg tcgttcttaa gaaggacggc ttctttttta
      721 tcgaaaatct gattcgtcta catgactaaa gtcgcgagta ttctctgctt ctcgataaat
      781 taaataacgt cattactttg aggaaagcta taaatcaata tatttcagct tttccttatt
      841 tatgtactac cttttactta ttaattataa ggtagtacat atatatcttt ttttggtagt
      901 aactaaaaat aaatatgtta taatagtatc aaatgaatat tgctaaaaga aaagaggtag
      961 gaacgtggta gcgaagaaat atgtaatcgg taattttaaa ggtggcgtag gtaaaagtac
     1021 gtgcgcgcaa atgttagggt ttgaatcagc agttaatgaa ggaaaaagaa ccttgataat
     1081 tgacttggat atgcaaggaa atacttctga tgtgatgaac ttaacgcata tgaacttcac
     1141 taacgaagaa ggaggcggag aaggtgaacc gattgtttac gaaaatactg tgactgacgt
     1201 gttgataaac aatcttccac ctaaagaggc tgtctataaa gtaatcaata acctctatat
     1261 acttccagca gacatgtcct ttgaaatgta tgatgattgg atcaaagacc aatataatga
     1321 ttcattatcc caattcaaat atatgaaaga aaagctagaa ccattatttg atgagtttga
     1381 cgtcatctat ttagatgtcc caccttctat ttctgtatat agtaaatccg cgatgtacat
     1441 tgcggattgg gcaatcgtcg tcttacagac ccaagtaaaa tcgatgagaa atgcgatgca
     1501 atatcttgag tatatggact tttttgtgaa agaatttgat acaaatttat atgttgctgg
     1561 tgttattcct ttcatgttgg aaagcggtga cgcagtagat caggaaatgt ataaacaagc
     1621 ccaagagatt tatggggaac atttgattaa aaatgttgta ctcaaaaatg cgcgcttaaa
     1681 acgatacgac ggatcaggta ttacgatgga gaagacaaag aaaggtctct taaaacaatg
     1741 ggatcgcaaa agccacgaat tatttatcag tattttgaaa gaattggaag aacacgagaa
     1801 ttggtatgcg gaggtggagt aattggctga catcaaagga cttgatattt ttgggaacaa
     1861 caaaggactc gccaaaataa aaaaagaagc agaagcaaag aaaaagcagc aagcaattac
     1921 accatctgaa aataagcaat tagaagtgca ccaagttcaa gaaataaaaa cttcctccaa
     1981 tgttgaaaga caga
//
LOCUS       DAFGZE010000183         1951 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.188, whole genome shotgun sequence.
ACCESSION   DAFGZE010000183 DAFGZE010000000
VERSION     DAFGZE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.188"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..522)
                     /locus_tag="LTU54_003088"
     CDS             complement(<1..522)
                     /locus_tag="LTU54_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBL8297720.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIP"
     gene            1175..1270
                     /locus_tag="LTU54_003089"
     CDS             1175..1270
                     /locus_tag="LTU54_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HBL8297721.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
BASE COUNT          625 a          387 c          311 g          628 t
ORIGIN      
        1 tgggatatcc attaagctcc taaactcttc aatacgaact acataataac cagtagaacg
       61 gtactgcata agcagtcgga atagagtttt tgcatacgta gaacgcaacg acgtgaaagc
      121 agataattca aatctactaa attcagctgt taaaccatta ataatatatt ctaggtccgg
      181 attaacactg acttcaacaa acttttttgt tttattgatt tcaaaaccag taaacaaaac
      241 aaagtagcga atagaatcct catcttcagt acggtaagta agattaagca tatctttata
      301 gagcttttct aaatcaccga caaaggcttt tgtagcagtc atcttataat cacttaactc
      361 ttttaattcc tcgaaagaaa atctaacttt tttcaaacca cgatctttca tacgagcgca
      421 taatgcaaaa aataaatcca tctgcttagc attgaaattt ttcagcggta ctaaattcaa
      481 ttcattttga tatttgactg ttaattccgt tttttcatcc attcctttct tcccccctta
      541 caaataaata ataaccttta acaaccaact cgtaaatagg ttggttgtta ttatacaaaa
      601 ggatattagt actcagataa aaagttgtta gtactcagat aaaaagttgt tagtactcag
      661 ataaaaagtt gttagtactc agataaaaag ttgttagtac ccttctgtat ccgttgggag
      721 agtaaggctc aactgccccc taaaaagggt tctaaaaaat ctttaaaaaa taaataaaat
      781 aaacgcgcgc gcgcgagcaa aatttcccgc caaaaaatca cccacttttt aaaataaaca
      841 ggttcgcgca tgatattaaa ctgccaatct agctccctat ggtcgctgta agaaggagtg
      901 atcgctacgc tctcacattt tacttgcagc agtcgccttt aggctccttc gccaaaaata
      961 attcattagg gactcctttg tggcgtcctg agagctcgtc taagccgttc taagacaaga
     1021 ttcgataaaa actatgtcct tcgttctaaa gcgccttaca ggcggtcagg gcaagcccta
     1081 acaaccccaa ggcaataaaa aacttaggct tatttttgct tttttattgt ataattgatt
     1141 gtcgcctatc cgacagtctt gcgactggaa ggaggtgtgt ttcgtgttca ctaactttat
     1201 ttgctccctt cttgttgggc ttttaagcgc tttgttcagt tactggttga ataaccgaaa
     1261 caagaagtaa aacttaatta aggcgactca gcccacaaaa aaagccccag agaatccgac
     1321 ctctctgggg atttcgtgtg tttcgtgttc acttatttgc aatcaaatta taacagaaat
     1381 aaatatttat ttcaaaaata tcctacaaaa gtctaaaatc cgtaattatt tcttatatgc
     1441 aacatttaca aataaagtcg ctctgagagc tcgtattttt cgatttaaga cgttttattt
     1501 agtttttggt gtatttcatt ggtccaagct aaaaaactct tagaagcgat tttagagcca
     1561 tttaaaccca cttaattata ttctccatga aattcgctcc atgcgtcaaa agctgcgtct
     1621 gaaattccgc cgtcttcgtt cactagttgc attaattttt ccaacgatcg ttttctttcg
     1681 ggtgtcatat ttttatattc caacaatttt tcgtctgtta aatttggtta tcgattacac
     1741 gtgcagcact cccccccgaa ttttaaagag cataaggaaa cgtgtaccca ctttctaaaa
     1801 aaagtgggta gataggctgt gcctatcacg tttctcagaa aaaaatttga tatcactttt
     1861 tcgccatcgt gggtagctta tgtatagcat tttggcaaaa aacagctata caaatatagt
     1921 atttgtatgg tatacataaa ttatacataa t
//
LOCUS       DAFGZE010000184         1918 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.189, whole genome shotgun sequence.
ACCESSION   DAFGZE010000184 DAFGZE010000000
VERSION     DAFGZE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.189"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(652..942)
                     /locus_tag="LTU54_003090"
     CDS             complement(652..942)
                     /locus_tag="LTU54_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297722.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1150..1841)
                     /locus_tag="LTU54_003091"
                     /pseudo
     CDS             complement(1150..1841)
                     /locus_tag="LTU54_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
BASE COUNT          523 a          367 c          294 g          734 t
ORIGIN      
        1 taacttaaaa gttatctttt aaatacttaa ttgttaaata ccttatttga tgcaaaagaa
       61 atctacgata gtcaacaaaa aaattttcat tatatccaga aaaatttttc ttagttaagg
      121 acctatggat tgcagtaatg cagaattcgt cgaataaact gccaaagaca tacaatcata
      181 ttgcttttaa aattgacgaa caagatatag attcgtttgt ctcaaaaatc caaatgttag
      241 gtttaactgt cgaaccagga cgttctcgtg tgaaaggcga ggcttcttca atttactttt
      301 acgactatga taatcacctc ttcgagttac ataccggtac tctccaggaa cgtttaaaat
      361 cctacaattc cactacctga gactagtaat gactagtctc tttttatggc ctctgatccc
      421 cccattgagc gaatactaat agggcttcga attgatctgt tcaatcttta agcaatatcc
      481 ctcaaatgag tttggaaagt ttagtgattc atcacaaata cttaaataca tgtagttata
      541 tatagattga ggttcttgtg agcgtatgct cgtgtgggtt caaatcccat cgtctgcatc
      601 catctttcca aaacaagcgg ctaggacatt tttgtctcag ccgcttgttt tctagtttat
      661 tctttcatct tgtcggacct tttttcttgg gtatagactt ttctcatatt ctaccacaat
      721 tttgggtttc cctaacacat gataggatct cacaacaaat tgctttctat tattataata
      781 acctgtaacg actaacttca tcccttcgtc tacttcagct aggaagttca aactatggga
      841 agcaatcaaa caattgaccc catttaaact gaaacggatc aacggtctgg cactaacttg
      901 caaagtacgg atcttatcta ctgttccata ttttttctcc ataaccttca cctcaatacc
      961 attatacgaa catacattcc ctttgtaaag aagaaactct gttttatctt tttgactttt
     1021 tgtctattac ctttcttaaa atcatattgg cacttcctgc gaagatgaag gacatgtact
     1081 aaaaaaaaga ggcagcagta tagattactg ttgcttcttt actaatttag gtttacttgt
     1141 taagaaagat tatttacgca aatgttcttc aacaatttgc tgagatttat cagctgtatc
     1201 ttccacttca tcttttgctt tttctaactt ttgaatatat tgtttcaagc ttgagttttc
     1261 agcagttgct tcgtctaatt tcttttgtgt atctgttaac tgatttctac atttgttttt
     1321 tcttgtgtca gtgttgcgat ttgttgattc aaaccattta cagtattttg taattctgaa
     1381 agttttgctt gtgcttcttg tagtcttgct gtccaatctg cattcagatg attgatttca
     1441 tcattttttt ggttgatctc atctagtttg acattgattt cattttgttt gctttgtagt
     1501 tgtgcatcct tgctgactaa ttgactttct aagttttgtt tttctgctgc cgattgattt
     1561 ttttggtttt ctaattcatt tacttttgtt tggtattctt ggacatctgc ctttgaatct
     1621 tgcaatgtct gttctttttg tgctagtcga gcggtaagat ctgtaatcgc ttgctcttta
     1681 tctgtcaatg tatgatcaag ctgtgtcaga ttctcattga ttgtatcaat atctgtctct
     1741 ccaccccaca aaataatttc atttgcgtac cgcgtgcctg tgtagccaat tagacataga
     1801 aatgcaacga caatcccacc agtaataaat ttctttttca tttgcatagc tccttaatcc
     1861 aatactttta catgctaaaa gtaagtgatc tcaaacacaa tgtcaagcgt ttttaaat
//
LOCUS       DAFGZE010000185         1907 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.190, whole genome shotgun sequence.
ACCESSION   DAFGZE010000185 DAFGZE010000000
VERSION     DAFGZE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.190"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            522..854
                     /locus_tag="LTU54_003092"
     CDS             522..854
                     /locus_tag="LTU54_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBL8297723.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1473..1814
                     /locus_tag="LTU54_003093"
     CDS             1473..1814
                     /locus_tag="LTU54_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HBL8297724.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          659 a          257 c          412 g          579 t
ORIGIN      
        1 ttttcaggca atacgaatat tattcgctta cattttttgt ataaatttaa ttgtctgcta
       61 tgcttgtgat gaattgattt tcgttttgca cttttggatg ataaatttgg acaattgaaa
      121 atacaaaaaa aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt cagcgttcat
      181 acacggcatt aagatttggc ccttaatgcg ttaacctttc agtgttacgg tattacccgc
      241 aatggattta gtataacaga tattcaaaag tgataccact ttttttggct gtgttaaggt
      301 taaaaatacg tgatattacg atgaatctat gcctaagcat aatgttataa aaatgtccgt
      361 gtggctcttt gccgtatgac gaacgctgaa attagaggga acgcatgttt ggatgcgttc
      421 cctctttttt gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa taacaagaaa
      481 aacttgttaa aaatatttaa agaaattggg tggtttattt tatgtcatat acaggaattt
      541 tgagtctcaa agatatttgt cactacggta aacggtgtac cgctacagag aaaatcacga
      601 agaaactatc aactggccaa agtaaaaccg ttgtgcagtg taagaaatat ataatccaga
      661 aagacaaagt ctcagaggaa atgatttatt acattgggaa acaaaagcaa ataattttaa
      721 aagatcctat ccccttaaaa gagctatatc caacaatcaa acatgtttat gaccaaaacg
      781 gtgtactgat tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa gggatggggc
      841 gtttgatcgg ctgaattgat cattctcata acttgtattt tatcgttttt cgcttgcttg
      901 aaaatattgt cgagacttct aaaggaactt agagtcacta gaaaaataag gagtagactt
      961 gatctatcaa caattccctt cggggagtga cggggtaggt tttaacgatt ggtaacgtaa
     1021 aaaattggtc aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa
     1081 taataagcgc agagtaaaca agagggaatt tagaagttag acaagttaga atagcgtcta
     1141 gattaatcat gagcagctac taagggatct agactttgac gcaaactcat ggaataagga
     1201 gtaggtcttc tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg
     1261 tagatacttg cgaggacgtt gattattgga tcaatcgagc atttaccgct gtctactagg
     1321 gtttctcgtt tcggacgcca taaacgagtc gaaatttttt atgtattttt aagtaaacga
     1381 aaaacgataa aatatgaaca agtcctgaat aaagtgaaaa aattaatata aagcatagag
     1441 aaaatgaaat ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat
     1501 tgggcacaca tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt
     1561 ttagaagggg cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat
     1621 acgatcaaac attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag
     1681 gtaatgaatg gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag
     1741 atacttggtc atatttgagt gattatattg tcagctttga aatcattgaa tttgtagcgg
     1801 ataaagaaga ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac
     1861 gacgattaat aaaaaggtag tattacctta ggcattgagt aaggaat
//
LOCUS       DAFGZE010000186         1862 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.191, whole genome shotgun sequence.
ACCESSION   DAFGZE010000186 DAFGZE010000000
VERSION     DAFGZE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.191"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            376..1758
                     /locus_tag="LTU54_003094"
                     /pseudo
     CDS             376..1758
                     /locus_tag="LTU54_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          696 a          317 c          333 g          516 t
ORIGIN      
        1 cacgaatttt ataaatattt atggtgttaa tgacaaatga tatccgactc ctcttcatac
       61 gttctggact atttgctaat agattaaaat ttcagcatct atttctgacc ataaataaat
      121 gagctgccga aaagctgcca aatcagactt gaaaataaag gatcaaaaac ttttaagttt
      181 ctgaccccgt aactcacact tcaaaatctt acttaaaaag gagtctgaaa gaaaaaaaga
      241 gtattcaaaa tttttttcta actagctaca tcacatctat gcattgacac tcaaagaacc
      301 agaataagtc atcagaaagc aagggggtgc ggacataatc ctggacgaaa aaataaactt
      361 aggaggaatg tccgtgtgtt ttcccacaaa gagcgtgtaa gggcaattca gttattttta
      421 aaatacgatt gctcttatgc tgcaaccatt cgagaattag ggtatccgtc gattggtgca
      481 ctacggaaat ggtataaaga gtatttggaa tccggagagc tccatcagga gcaccgaaaa
      541 aaatcaaagt attctgaaga acaaaaacga atagcggtga atcattattt tgaatatggt
      601 caatgttatg cacgaaccat tcgcatgctt ggttatccta atagagagtc cttgcggcag
      661 cggtgtgaag aacttgcacc aggagctaga aaactccgaa aaagtgcggt aaagttaaca
      721 caagatcaaa aagaagctgt gttgaaaaaa ttctataaac cgcaaactaa taggaaaaat
      781 ttagccgaat ctggaggaat tagtagagtt actctatatc aatggaaaaa cacggttctt
      841 ggaaaggatt ttccattgtg tatgacttta aaaaatcagg aaaaacaaaa agagctactt
      901 ttaaaggaag tagaagactt acaaaaacaa gtacaccaat tacagctcga aaaagcatta
      961 ctcgaaggag cggctgaact gctaaaaaaa gaaaaaggcg tcaatcttct ttgtctatca
     1021 aatcaagaaa aaacgatatt gattgatgcc ttacgaaatc agttcacctt gaaagagctt
     1081 cttcaacagc tccagtttcc taaaagcagc tatttttatc aaaaacaagc acttgagaaa
     1141 ccagataagt attacaaaga acgtcaaatt aattatcacg attttcaatc ataacttctg
     1201 tgcgtatggt taccgtagaa ttcaccaagc tttaaaaaat atgggtaaaa agctttctga
     1261 aaaagttgta cgtagattga tgactgagga aaatctattt gttaaatttt ctagacgaaa
     1321 aaagtattct tcgtatgctg gggaaatttc tcctgcacac cccaatttat taaatcggaa
     1381 ttttaaagca aagaagccaa atgaaaaatg gttaacagat attactgaat ttagaattct
     1441 cgcagggaaa atctacttat caccacttgt cgattgttac gatggtgcta ttgttagttg
     1501 gactattgga acaaagccaa cttgcttcac tctatgtcta aaaagagtta ttcaccagat
     1561 aattcagcgt gcgaaggttt ctttggacga ttaaaaaacg agatgtttta tcaacgtgac
     1621 tggaagaata caacaatcaa tcagtttata aacaaattgg atgattatat tcattggtat
     1681 aataatcaac gaattaagtt atctttagga aggctaagcc ctcttcaata tcggaagaag
     1741 caaagatata tttgttaaaa aggtccaaaa aaaatatccg cacccccttt tttttgtaaa
     1801 aaactattgc acaatacaag tagcctacgg ctatcgaaat ttccataact ttcgagcaag
     1861 aa
//
LOCUS       DAFGZE010000187         1751 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.192, whole genome shotgun sequence.
ACCESSION   DAFGZE010000187 DAFGZE010000000
VERSION     DAFGZE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.192"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            197..541
                     /locus_tag="LTU54_003095"
     CDS             197..541
                     /locus_tag="LTU54_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297725.1"
                     /translation="MLFERVKELCKKRGISISELENRIGFGKNSIYKWKTQSPKIETL
                     QKVADYFHVSTDYLLGRTDDPNAGVAPEERKLTVEEALASVMSSDGKPLTDNDREILS
                     AMIEAYLEKKDN"
     gene            577..972
                     /locus_tag="LTU54_003096"
     CDS             577..972
                     /locus_tag="LTU54_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HBL8297726.1"
                     /translation="MIINELGVKVEERENLDADGHYVACMNTIVIKANLSKYRRQRTL
                     LHELGHASKHHDNYFLYNLAFSLHSKMEYEADRFMIEKLLDRYIAKSELEPHNINYMK
                     FIEDNNLSVRFEPLVKELLKARIYCYAAL"
     gene            1025..1336
                     /locus_tag="LTU54_003097"
     CDS             1025..1336
                     /locus_tag="LTU54_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297727.1"
                     /translation="MIYTEFKEWLEKSTTGYETFIIKATNYQIEKNKNRPQKKRWDDK
                     KIDKAVLEMWKQVVTNLYQTIRKEKGVPLINGKEIWLEFIEEQGLIEFFNDSMAELEF
                     E"
     gene            1348..>1751
                     /locus_tag="LTU54_003098"
     CDS             1348..>1751
                     /locus_tag="LTU54_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HBL8297728.1"
                     /translation="MAMIKQYKKKNGEKAWYFKTYLGIDPLTGKKKYTTKRGFRTQKE
                     AKTALSRLELELQKTGMPTSTNTTFKEAAELWLESYKKTVKESSYSRTKIIFNKHIYP
                     KFGNIKLSKINTAYCQKVVNDWSEKGTSKQYPL"
BASE COUNT          715 a          245 c          305 g          486 t
ORIGIN      
        1 agaaaaaccc taaaaattaa cattcttagt ttgccgacct cgaattcgtt tattttatta
       61 ggtgtatttc ttgttgcttg ttagcttatt aaattagctt acgagttaaa tataagacaa
      121 ttggatagat ttgtcaacta cctttctatc tttttgtata aattattatc gtacgctcta
      181 ggaaggttga tttgacatgc ttttcgaaag ggttaaagag ctctgtaaaa aaagaggtat
      241 ttctatttct gaattagaaa atagaatagg atttggaaaa aactctattt ataaatggaa
      301 gacacaaagt cctaaaatag aaactttgca aaaagttgct gactacttcc atgtatcaac
      361 agattatttg ctaggacgca cagatgatcc taacgcggga gttgcaccag aggaaagaaa
      421 actaaccgtg gaagaagctt tagcatctgt tatgagtagc gacggaaaac cgctcaccga
      481 taatgatagg gaaatactat cagctatgat tgaagcatat ttagagaaaa aagataacta
      541 aataagtagg tgagtcattt ggacagtcaa attgaaatga taattaatga actcggcgtt
      601 aaggtagaag agcgtgaaaa ccttgatgcc gatggccatt atgttgcttg tatgaatacc
      661 atagtaataa aagctaattt atctaagtat agaagacaaa gaaccttatt acatgaatta
      721 ggacacgctt ctaaacatca tgataattat tttttatata acttagcatt ctctctccat
      781 tcaaaaatgg aatatgaggc tgatcgcttc atgattgaaa aattattaga tagatatatt
      841 gcaaagtctg aattagaacc acacaatatc aattacatga aatttataga agataacaat
      901 ttaagcgttc gcttcgaacc acttgtgaaa gaattattaa aagctcgcat ctattgttat
      961 gcagctctct aaaatttttt aagcaaaaaa gaacatacgt tcaaaaatag aaaggtgaac
     1021 aaaaatgata tatacagaat tcaaagaatg gttagaaaaa agcacaaccg gatacgaaac
     1081 atttatcatc aaagctacta attatcaaat tgaaaaaaac aaaaatagac cccaaaaaaa
     1141 acgctgggat gataagaaaa tagataaagc tgtattagaa atgtggaaac aagtcgtgac
     1201 taacttgtat caaacaattc gtaaagaaaa aggagttcca ttaattaacg ggaaggaaat
     1261 atggcttgaa tttatagagg aacaaggact gatcgaattt ttcaatgata gcatggcaga
     1321 attagaattt gaataggggt aatattgatg gcaatgataa aacaatataa aaagaaaaat
     1381 ggcgaaaaag catggtactt taaaacttat ctcggtattg atccgctaac tggaaagaaa
     1441 aaatatacta ctaaacgagg atttagaaca caaaaagaag caaaaacagc actttctagg
     1501 ttagaactag aattacaaaa aacaggaatg cccacaagta caaatactac tttcaaagaa
     1561 gcagcagaat tatggctaga aagctacaaa aaaactgtaa aagaaagttc atattcaagg
     1621 actaaaataa tctttaataa acatatatat cccaaatttg gaaatattaa gctttctaaa
     1681 attaatacgg catattgtca aaaggtagta aatgattgga gtgaaaaagg aacttcaaag
     1741 cagtaccctc t
//
LOCUS       DAFGZE010000188         1733 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.193, whole genome shotgun sequence.
ACCESSION   DAFGZE010000188 DAFGZE010000000
VERSION     DAFGZE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.193"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..903
                     /locus_tag="LTU54_003099"
     CDS             <1..903
                     /locus_tag="LTU54_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HBL8297729.1"
                     /translation="GFTKQSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSY
                     PLVGPATWDGKVYVQNLFNAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGF
                     KACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESL
                     IGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGW
                     KVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELG
                     MICGKLDRQYYSASAISSIVIPPK"
     gene            916..>1733
                     /locus_tag="LTU54_003100"
     CDS             916..>1733
                     /locus_tag="LTU54_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HBL8297730.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETRIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETV"
BASE COUNT          582 a          252 c          396 g          503 t
ORIGIN      
        1 ggatttacga aacaatctgc atgtggaata ttggggaaca ttcaacaaga aacaggagga
       61 acttttgatc ctgatactgt tcaaataggt ggaccagcat acggattagt tcagtgggat
      121 ggttcttcat atcctcttgt tggtccagca acatgggatg gaaaagttta tgttcaaaac
      181 ttatttaatg ctgcaggtat taaagaacca ataacgagct tagatgcaca agttcgattg
      241 cttatttgga catttacaaa tggacaatgg atgggcgtag tacaacctac gacggttgat
      301 ggatttaagg cctgtactga tccaagacaa gcagcatatg cttttgaacg aaactatgaa
      361 cgtccggcag cgacacatcc tgaacgtcaa gattatgcag ttaactggta taacaaattt
      421 aaagatttaa aaccaggagg agctactgga gaagcaggac taaaacattt agagtctttg
      481 attggacaac gaattggcaa tggtcagtgt tatggcttgt ctgcagaata ttcaggatat
      541 ctgggtggtt gtggcatggg tgctggaaca aaatatggtt taactcatgt gataggaaat
      601 acttctgcag catctgatat tggtattgcc tatgattggt ctgctgttgg ttggaaagtg
      661 attcaaaacc ctagatatga tcaattagta gttggtgcaa ttattaattg ggcaagaggt
      721 ggacaagtgg gttcatggtt tgcagacgga acttatggac atactggcgt tatccgaggc
      781 ttagctaatg gtcgtatgca aacttatgaa caaaacacag agttaggtat gatttgtgga
      841 aagttagatc gccagtatta tagtgcaagt gcaatttctt ccattgtcat accaccaaaa
      901 tagggaggtg atttaatggc aaagtggaat gtcatattaa gtacaacaga accctataat
      961 tacgtgggga tgattcaagt tcgacaaggc aataaaaaca ctgaagttat ggaagctaca
     1021 attgtcgaaa atggtcttcc atatgattta tcagaatgta aggtatattt tgaatcagtt
     1081 gttggcggaa aatatccagt ccaattagaa acaagaattg tggatgctaa aaaagggaaa
     1141 atcaaatata tttttgataa atattccatg cagtgtttgc atcgacaaac agccaatttc
     1201 attatattta aaggagaaga cttgattgga acaactcaag acttctccta ttttgtcatc
     1261 aatgctgttt caaaaacaga aggagaaatg ggttcttatt ggcaatcaat tgaagattta
     1321 attgctgata tgacagattt tattaatgaa aataaaggcg attttacgga ctggatgaat
     1381 gaaaggaaag aagaatttga tcgctggaga gaagaacagg agaatagttt ccaagattgg
     1441 agagaaggac aagaatcaga ttatttgtca tggtttgaat caatcaagga tattttgaag
     1501 tctatcgatc caggtggagt aatgttagcc gaattaatgg atgcacgtgt agatcttcaa
     1561 ggtgtacgcc atgaatcgat ttctgaacgt ttcctatccg atctaaatta tttgtatcaa
     1621 aaaatgaaag cagcactttt cacgattgaa tacggtgaaa ttgaagtgac tgatattctt
     1681 caagacgatc tcttttcaga taatcacgaa gtcgaaaaaa ttgaaacagt aga
//
LOCUS       DAFGZE010000189         1673 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.194, whole genome shotgun sequence.
ACCESSION   DAFGZE010000189 DAFGZE010000000
VERSION     DAFGZE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.194"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(113..490)
                     /locus_tag="LTU54_003101"
     CDS             complement(113..490)
                     /locus_tag="LTU54_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297731.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(790..1671)
                     /gene="galU"
                     /locus_tag="LTU54_003102"
     CDS             complement(790..1671)
                     /gene="galU"
                     /locus_tag="LTU54_003102"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBL8297732.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          468 a          370 c          245 g          590 t
ORIGIN      
        1 aaaaagattc ttttatataa gtgaaaaaag cttttttcca ccaattaaat aaatctctca
       61 tctcaatagt ttatccatta ttttatcttt ttttgcgtat acctactaaa tattaccgtc
      121 ttcttctcgt tcttctactt tgttcgagag aatctgtact agaaaaagta gttgtatgtc
      181 taccttttct gcctatccga gactttcttt tcatatatgg gatataaatg attagataca
      241 gtacagcaaa aaataaagca aatacgcaag cttcccaact acctaacgcc aattcaacca
      301 tcaaggtagc aaccccaacg gatgctgcat accagataaa aatcagaaac gtttcttttt
      361 ttctgaccat gcgaaacatg ataaatagac taataagcaa tagtggcaac acaaatatcc
      421 aattgcttcc ttccatcagc cacttgagta tatcaagcag cgactgtgca acagcgacta
      481 attttcccat agtgaacaca cacctttttt tataattaaa aaattcgcct ttttattcta
      541 ctatgaatct tctggcttgg ctttcatttc tcttatgtaa tcattttctt ttatacaact
      601 taaaaaaaaa ttaaaccatt agcataatat tttctgaatc tattcacgaa agtctttttc
      661 tatctttcgt tttccacgac tcaactgatc tagagtcaaa aaactaaaac gacagttgcg
      721 caaatgacgc acaagctgcc gctttaataa caaatagcct ttcattctca gcaggccact
      781 tatttccagt taactttctg ttcttagctt tttactcaac tggataatat atgcacttaa
      841 tgaagtccca atttccgaat gttgaagtcc atattcaata tttgtcttca tatatcctaa
      901 cttatcgcct acgtcgtatc tttttccttt aaactctctc gcatagaccg cctctgtctg
      961 gttaagagtc tgaagtgcat ccgttagctg tatctcgttt cctgctcctg gttcttgagt
     1021 ttctaatatc tcaaatattt taggagtcag tagataacgt ccgataatgg ctaaattact
     1081 tggtgctttt cccatttcag gtttttctac aaaccctttt actctgcaac gacccttttc
     1141 gctatctgtt ttaggatcaa taattccgta tttagatgtt tcattttctg gtacacgcat
     1201 gacggcaaca gcggaagctt gtgtttcttc ataatccatc atcaattgtt gagtcaatgg
     1261 gatatcatct ttcatcaaat catcccccag catgacgaca aacggttcat ttcctataaa
     1321 tgcttttgct tgaagcacgg catcccccag tcctcttgga tgagattgac ggatgaagtg
     1381 aagattgact tgagtcgttt cttcaactaa agcgagtaat tctgtttttt ccttttcccg
     1441 caaattgttt tccaattcga tatttgcatc aaagtgatct tcaatgggtc ttttttcttt
     1501 tcccgttacg atcaagatgt cttcgattcc cgaatccaat gcttcttcca caataaactg
     1561 gatcgttggt ttatccacga ttggaagcat ttcttttgcc atggcttttg ttgccggcaa
     1621 aaaacgtgtt cctagcccag cggcaggaat gactgctttt ttgaccttca ctt
//
LOCUS       DAFGZE010000190         1644 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.195, whole genome shotgun sequence.
ACCESSION   DAFGZE010000190 DAFGZE010000000
VERSION     DAFGZE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.195"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..351
                     /locus_tag="LTU54_003103"
     CDS             <1..351
                     /locus_tag="LTU54_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297733.1"
                     /translation="QDLIYYVHEDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQM
                     ESFQATIDLNEELVKKLKDQLPYQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQ
                     KLQEKIDRMTKEKI"
     gene            373..603
                     /locus_tag="LTU54_003104"
     CDS             373..603
                     /locus_tag="LTU54_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297734.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            1149..1424
                     /locus_tag="LTU54_003105"
     CDS             1149..1424
                     /locus_tag="LTU54_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297735.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            1466..>1644
                     /locus_tag="LTU54_003106"
     CDS             1466..>1644
                     /locus_tag="LTU54_003106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297736.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNA"
BASE COUNT          689 a          215 c          284 g          456 t
ORIGIN      
        1 caagatctga tttattacgt gcacgaagat aaggttaaag atcaagtgaa agatgaagat
       61 tatgaacaac acgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagct
      121 caaaaacaaa tggaatcttt ccaagcaacc attgatttga atgaagaatt agtgaagaag
      181 ctaaaagatc aattgcctta tcaagtcgca gatgatcagg aaaatactga aaacaagatt
      241 aatagctacc agcaagaaat tgaaacatcg aaaacgcaac tgaaagatca agaagagatc
      301 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaagattta gaaggacagg
      361 tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag atactttcgc tgagcagaaa
      421 gtattgatta aagcctatct caaagttgta aaagctaaag aaatatgtcg agaagtgaat
      481 ttatctaaca caacaatatc tgagttgaag aatggtcgaa agaatttaga tactttaaaa
      541 ttcagatcat ttgaagctct atatgtagct gctaaaaaat attttacagc tgataacaaa
      601 taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa aatgcttatg aatcagctat
      661 atcaccttaa ccatttgtta atgagatggg agaaaacatt aaccacgatt ctaacgcaaa
      721 catcaagcac ataaaacatt taacactcct cctttctggc tgaagatttc aaccaaacag
      781 gaagacagcg tatcaattat agcatacact ttttcttaaa ttcatttttc atatgacaaa
      841 atatttcctt ctaatcgaca aagttagaag gattttttat tgtctaaata cacaaataac
      901 cttaattttt aaagagcata aggaaacgtg atatcacttt ctcacgaaag gtgttaggta
      961 ggctgtgcct accacgtttc taagaaagat aaatgatatc aaaattttgg atttgtgtgt
     1021 agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa taagcgtttg tgtgtagcaa
     1081 aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc cctacacata ataaatttgg
     1141 aggaaaatat ggacttatta attagaaata ttgaagaaaa gtatattaat aaaattgaca
     1201 aacgttgtga agaacttacg gtcaagactg gcaaaaaatg gagtcgaaat caatacctaa
     1261 aagttttgat cgaaaatgat tttgatcacg ctcttctgaa ttataaaaaa gaccaatttg
     1321 atcggttgtt agaaaaattt gtggatattc aaacttataa tacgaaaact ttagaagaat
     1381 atattgcaac aaataatcaa ttaattggtt tgttaattga ataataaaaa tagtattgtg
     1441 aatttataaa aggaggaaat caattttggc aaaagcaaca agaaaacatg tatatcttta
     1501 cgatatgcat atcgaagtac ttgatgaaat aatttctact catgaagata tatctaatta
     1561 tagtgaagca gtcagacatt tgtgtatgat gtatgatcca aaaatcgaag aaaaaataaa
     1621 tccaattcaa attaaattga atgc
//
LOCUS       DAFGZE010000191         1642 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.196, whole genome shotgun sequence.
ACCESSION   DAFGZE010000191 DAFGZE010000000
VERSION     DAFGZE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.196"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..217
                     /locus_tag="LTU54_003107"
     CDS             <1..217
                     /locus_tag="LTU54_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBL8297737.1"
                     /translation="YEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPP
                     ENMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            250..1158
                     /locus_tag="LTU54_003108"
     CDS             250..1158
                     /locus_tag="LTU54_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBL8297738.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1155..1403
                     /locus_tag="LTU54_003109"
     CDS             1155..1403
                     /locus_tag="LTU54_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL8297739.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(1406..>1642)
                     /locus_tag="LTU54_003110"
     CDS             complement(1406..>1642)
                     /locus_tag="LTU54_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HBL8297740.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT          571 a          244 c          352 g          475 t
ORIGIN      
        1 ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat atcatagaac
       61 actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc agattgtgga
      121 gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa tgcgacgccc
      181 aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa ataaacgagg
      241 agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt agcagaacag
      301 gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa tatacctaaa
      361 gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc gtttatatct
      421 aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc ggaggatatg
      481 gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga tgattacaat
      541 aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa gggcgataaa
      601 ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt tccgactgat
      661 atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg caatgaattt
      721 tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt atttgctatt
      781 gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg gaaggtcggc
      841 atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga aaggtatata
      901 tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta tgaaaacata
      961 tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga ggtggcggaa
     1021 aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac cagggacatg
     1081 tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc tgatatagaa
     1141 gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat atcgataaac
     1201 ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac gagaattgga
     1261 cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac gaagaggatg
     1321 aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag ataatatatc
     1381 ttttaacccg tggtgctagt taattttaat atcctaaaga atatctcaaa gttcctccaa
     1441 aacgtcgttg tgctcgactt aatagaacac tgtaagccag taatatgctt tcaaatctac
     1501 tctctagccc tttaacagaa cgtgagttga aattttgaca acctaatttt tctaaggaag
     1561 aaaaaacagt ctcaactgtt tttctttgtt ttccaagtaa gctgtggtct agtttttctg
     1621 atttatccat gtttttacgt gg
//
LOCUS       DAFGZE010000192         1635 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.197, whole genome shotgun sequence.
ACCESSION   DAFGZE010000192 DAFGZE010000000
VERSION     DAFGZE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.197"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(594..>1635)
                     /locus_tag="LTU54_003111"
     CDS             complement(594..>1635)
                     /locus_tag="LTU54_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBL8297741.1"
                     /translation="AIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVENL
                     SIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDLV
                     DSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLIV
                     HSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFSA
                     SFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGGA
                     TFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRDV
                     INDDSKVIDVNA"
BASE COUNT          514 a          357 c          289 g          475 t
ORIGIN      
        1 atctgttcta acattctcaa ctttgtcagt ttaccatagg aaaatgggaa aatctacgaa
       61 agctctcaac agaatcgtcc tacttaaata cgcttttctt taataataaa taagttggtt
      121 gtttttgtta tttgcgcttt cacttttcgg aataaataat gtgtatttat atggaataaa
      181 taaaaaaaga ggtcgtgact aaaaacaagt cacaacctct ggtaaacggt gttctcctga
      241 caactcctcg gtcacaagac acaatattca acaaacatga gaagctattt tttgaaattg
      301 ctctttgtga ctcagagtga aacggcaggc tcacgacctc tttcttaaac ggtgttcaaa
      361 agcttgcatc tgcggtaata agctcaacca aacgtaaaat atgaggaact atttttgttt
      421 ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttattc acggtgtttc
      481 agaagtagct tctcggaaat aagccgaaat ttcacaaaaa tttgagaaac tatttttagg
      541 aaacttcttc ttatttctta aagctgacca cttctatcac aacctctttt tgactatgca
      601 ttcacatcga tcactttaga atcatcattg atgacatcgc gatctaaatc tttgatgcgt
      661 gcacttgtca cgataccagc taccatgcta ccgctgacgt ttactgctgt acgtgccata
      721 tcgattaatg gttcaacaga gataactaac ccaacgattg ctactggcaa gttcattgcc
      781 cctaaaacga tcagtgaagc aaatgttgcg cccccaccga ctccagctac accaaaagaa
      841 ctgattgtta caattgccac aagcatcaaa ataaattcca aactaaagac attcactcca
      901 gctgctggtg cgacgatcgt tgccaacata gctggataaa cgccagcaca gccgttttgt
      961 ccaatagaca aaccaaaact tgctgagaaa ttagctgttg catcatctac tcctaaagct
     1021 tttgtctgag tctcgatatt caatggcaaa gctccagcac ttgaacgtga agtaaacgca
     1081 aagctcaata ccggaaaggc ttttttgaaa tagttgattg gattgacttt tgcaccaatc
     1141 aaaatcagtg agtggacaat caaaacagca agcaatgcta cgtaagaagc aacgacaaat
     1201 ttacctaaat tgacgatagc tgcaaaatca cttgttgcca aaacgtttgt catcaaagca
     1261 aatactccat aaggtgtcaa acgcaaaaca agcgtcacga tccgcataat gattttatac
     1321 agactgtcga ctaaatctga aaagaatgct gcttcttttg gcgcttttct tttcacgcct
     1381 agataagcga ttccgacaaa tgccgcgaaa ataacgactc caatcgtact tgttgcacga
     1441 ctacctgcta aatcagcaaa aacgttttta gggataaagg ataaaatctg ctgtggaatc
     1501 gataagtttt caactgtttc ctgacgtgtt gccagttcag agattcgtgc tgtttctgca
     1561 gctccttgtg tgaatttagc tccttggagt ccgaacagca ttgttgaacc aattccgatc
     1621 aatgctgcaa tagct
//
LOCUS       DAFGZE010000193         1609 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.198, whole genome shotgun sequence.
ACCESSION   DAFGZE010000193 DAFGZE010000000
VERSION     DAFGZE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.198"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(53..508)
                     /gene="tnpA"
                     /locus_tag="LTU54_003112"
     CDS             complement(53..508)
                     /gene="tnpA"
                     /locus_tag="LTU54_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBL8297742.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            751..1609
                     /locus_tag="LTU54_003113"
                     /pseudo
     CDS             751..1609
                     /locus_tag="LTU54_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          523 a          307 c          305 g          474 t
ORIGIN      
        1 aggggatttt taaaagaccg ccaactgctc atcttcactc acttacttct ctttattttt
       61 taaatggttc tacgtactct cttttactta taatatatcg aatgcgatca tcagcttctt
      121 gttcacggat gtatttttca tttgtctttt gattcaatcc gactgtactc acatagtatc
      181 tcttcgacca aaaggatttg tttccgtatt tatatttgag atttgcgtgc tgctcgtgga
      241 ttagtaccgc actacgacct ttcagatacc ccataaaact ggatatacat atttttggtg
      301 gtactcgaac aagcatgtga atatgatcta gcattgcatg tgcttctatg atttccacat
      361 ctttcatttc gcacaacttc ctaagtattt ttccaagatc ttttcgtaat tgcccataga
      421 ttacttttcg ttgatatttt ggtgtaaaaa taatatgata cttacaattc caacgagtat
      481 atgctaaact tttatcgtca tttgacatga aaaaactcct ttcgatctga tgagcggttg
      541 gtagccactc actagtatat cgaaaggagt ttttatcgct aaagatactt cttccacgcg
      601 tagaaacgcg cggttttctt gtttcgcacg ctttacgcac aaaactgact gaagtcatta
      661 agaaaataaa agggtctaaa acttttaagt tttgtaccga cccccaaaag ttagaccaaa
      721 aaatctaact tttggaggtc ggtatttttt atggctaagt atacttttga acttaaatta
      781 aagatcgttc atgattactt ggatggtaaa ggcggaagtg attatctggc aaagaaatat
      841 tccattaagg ctccatcaca agtgaagcgc tggatcaatg cctatcaaga atttggtgaa
      901 gagggtctag taagaaagcg acaaaagaaa aaatattctg ttcaatttaa gctggatgcg
      961 atagagttat atctaacaag tgagctgtcc tatcgtgaag ttgccaacac acttaacatg
     1021 aacaacccta atttgatcgc tagttggatg cgtcaattcc gtgagggtgg gatcgatgga
     1081 ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga aaaatgaacc caaaaataag
     1141 aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac gcatcgagga gttggagaaa
     1201 cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag aattgaggaa gctgcgaaaa
     1261 caggaagccc aacaacgacg aatgaagcaa tcgcacgaat catcgcaagc ctccgaggac
     1321 cgtttaaatt agttgagctt ctagaaactt taaagttccc taaggcaatc tttatgtact
     1381 ggcaaaaacg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaag
     1441 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
     1501 gtttctctat caataaaaag aaagttcagc gcctgatcaa gaagctagga ataaaagtaa
     1561 cgacatacac acgtaaatca cggcgctaca attcttatcg aggacaagt
//
LOCUS       DAFGZE010000194         1596 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.199, whole genome shotgun sequence.
ACCESSION   DAFGZE010000194 DAFGZE010000000
VERSION     DAFGZE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.199"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..1432)
                     /locus_tag="LTU54_003114"
     CDS             complement(<1..1432)
                     /locus_tag="LTU54_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBL8297743.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDD"
     gene            complement(1451..>1596)
                     /locus_tag="LTU54_003115"
     CDS             complement(1451..>1596)
                     /locus_tag="LTU54_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HBL8297744.1"
                     /translation="LGYAISPEYLFNVLADQAKQATFQLNDLNKAFLASHIYGKILSA
                     KFE"
BASE COUNT          493 a          300 c          220 g          583 t
ORIGIN      
        1 tatcgtccga tgttaattgg atttgataaa tccaagaatt caaaaagata gacgttattt
       61 gaagataata atagtcaaat tctatttgaa aatctctctc tatttcctta acatactgtc
      121 taaatcgaat ctcttgatag gtgggatatg ttaaagctga attattttgg atatcaacat
      181 tcttttgtct aaaatatcta tcatatttct caaaaagctg ctgcttatca gtgtctttct
      241 tccacgtttg ataaagatac tctataagtt cgttgatatt aaaaacttcg tctacttttt
      301 ccaaatcatc catttgaata tattttaatt cattctgtct tttcataacc ttcatgccgt
      361 tgaaattcac ccattgtggt aaatgataca tctgaatata aagttcttcc aaatcatcta
      421 agaaataaaa ggcattggca caggtcaatt gaatgatatt tttcaaattg ccaacatttt
      481 cactaaagcg atagtttaat aaaaaatcta ataattgttg agatatcagt attttctttg
      541 ctagtctttc ttcttctgat ttaaaaaata tcttgattaa ctgttttctt tcataaagag
      601 aacgactgtc taatgttgga agtgttagct ttacaggtat tcttctcaac aaagttgata
      661 atagtttatc ctcaggtggt tctgatgttg caaatataat ccagcaactt gatgtgtacc
      721 agttttcatt atcacccatc cgatgaaatt tcctcgtatc cataaactga aaaagtttct
      781 cctgacattc attgctcaat gcatgaattt catctaaaaa taagactcca ttattagcta
      841 attctgctaa accctttctt ccatcttcgg ctcctgtata tgcacctttt acatgcccaa
      901 aaaaattgga caagaaaagt tctggattat tggcatattc actacaatta actgaaatga
      961 aagatgcctt ctcttctata taattattgg ccaacaaatc cttatacaca taatttgcta
     1021 aatagctttt tccagttcca gtctcgccat gaagtaaaat gggtaagcca tttggcggat
     1081 agtagattgc tgctttaatt tgagcaatta catttcgcag acttccttga cttccgataa
     1141 ccctttctat tccaacccta aaagagctat cataccattt ttccgcttcc tctgaagagc
     1201 taaaaatttg ttgatttgga actttctccg gtaaagaaaa gaagattact ggacgagtat
     1261 tgatttttcc aaattctcca tcttttacga attcattcaa atattgagag acaatattac
     1321 gactaacatt taatttttca gatattttct gtgctgtatt gttcccccat ttatttttaa
     1381 tctcagagtt ctctatgaca taattgacaa tgcgttcttt taattcttcc atcacagcac
     1441 ctcctcgaca ctattcgaat tttgccgaca gtatttttcc ataaatatga cttgctaaaa
     1501 aggctttgtt tagatcattc aattggaacg ttgcttgttt tgcttgatcc gctaagacgt
     1561 tgaaaagata ctctggcgaa atcgcatacc ccaaaa
//
LOCUS       DAFGZE010000195         1550 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.201, whole genome shotgun sequence.
ACCESSION   DAFGZE010000195 DAFGZE010000000
VERSION     DAFGZE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.201"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..203)
                     /locus_tag="LTU54_003116"
     CDS             complement(<1..203)
                     /locus_tag="LTU54_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HBL8297745.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYR"
     gene            complement(435..692)
                     /locus_tag="LTU54_003117"
     CDS             complement(435..692)
                     /locus_tag="LTU54_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBL8297746.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(817..1149)
                     /locus_tag="LTU54_003118"
     CDS             complement(817..1149)
                     /locus_tag="LTU54_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297747.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT          491 a          276 c          203 g          580 t
ORIGIN      
        1 cgatacaaca tcgtttgttt gcttactgtg tcatattcct ctaaggattc atctgctaaa
       61 agaaccactt gttccaacaa acggtcggat aaatatttat agaaaatcag ccccaataaa
      121 taatttttat attctgacgc atccatttta ctacggagat tatctgccgc actaaataac
      181 ttctgattta attctgcacc caaaaagata cactcctatc tcttatttaa agttgctata
      241 taccctctta ttttagcata ctcagtttta gaaaaagcaa aaaaataaag aaacttattc
      301 ttgataacaa gctacgtgac gtatatccct aacacaaaaa tagccatatc ctttggtttg
      361 gatacggcta tttgatatta ctcattcaat tatcaaatca cagtaggtgt gaatctcatc
      421 gggatcggca atatctactt ctgattagga tcaaaggtga ttttctcaat aaaggtgtga
      481 tacaaagctt taacttgctt cttctcactc tggataagaa agcaatccaa actagtcaat
      541 atctgttgga catctttgac ttgaatcggt tccccttgtt gttgaaagat ccctaagatt
      601 tcattctctc gaatttgtaa ctgtcttcgt ttttcagtca attcatttaa acgatctttt
      661 aacatcggaa acaattcggg actatcttcc aaagcagctt cctgtttttg ctctaatagt
      721 tagattttgc gtaccatttt cttcttttat tttccttcga ttgctgattt taatatagtc
      781 taaaggaaat ttatatttat tgtattcttt ataacttcaa gagaacttcg tttgagtatt
      841 actgtatttt tttaattcca taagaaaatt agttattacc tgccacctaa tcagcaaatc
      901 tgaacctgag tcaatataat taatattatt ttccatctcc aacattgtaa gtactgttgg
      961 tgttaatata gggactttgt acaattgtat ttttttgatt ttatcatctg cacaaaggag
     1021 attttttaaa tctaatcctt cttttatttc aaaagttgaa ttttttaaat ctataagagt
     1081 aaaaccaatt ccttcatact gaatttctga aaatcgaatt tttctcccag ttgatagatc
     1141 aaggggcaga aaacctttta ttacattgtg agtgtcacca tcatttacaa tgtcattagg
     1201 aattttgatt ttagaattct gtggagcacc tattggaaga taataattca ttataatttt
     1261 gtagtcttca tgaggaaata aagtactttt aggattgcta agatattctc gtaccgaatc
     1321 taagaatggc ttatccattg aatcttcatc cccatttaaa ctaaaaaaca ttcctacaat
     1381 tgcttttgca actctaagtg aattcatttt ctttattgaa tgtgttaaat cagacttacc
     1441 attctttaaa cttgtattgt tataaaggaa atttttattg tcttctgcaa atctaatata
     1501 ttcctcaaca tactcactac caaaaaaact attacattta ctacataacg
//
LOCUS       DAFGZE010000196         1522 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.202, whole genome shotgun sequence.
ACCESSION   DAFGZE010000196 DAFGZE010000000
VERSION     DAFGZE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.202"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            12..1478
                     /locus_tag="LTU54_003119"
     CDS             12..1478
                     /locus_tag="LTU54_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBL8297748.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          417 a          289 c          323 g          493 t
ORIGIN      
        1 gggtatagat tatggaaaaa cagcaagaca agggcttctt cgggcagccg aagggattgt
       61 caactttgtt cttcactgag atgtgggaac gatttagtta ttacggtatg cgtgcattac
      121 ttatctatta tatgtacgat acagttgcca acgggggact tggattacca cgagcaacag
      181 cattagctat catgtcgatc tacggatctt tgatctacat gtctagtatc gtcggcggat
      241 gggtatctga ccgagtgtta ggagcaaaac gcactgtatt cttcggtgga gttttcatta
      301 tgcttggcca catcgtcttg gctttaccat ttggtgtaag tacattgttt gtttcgatgg
      361 cattgattat tatcggaacc gcctttttga aaccaaatgt ttctggcatg gtcggacatc
      421 tttattcaaa gactgacttg cgaagagatg caggattttc gattttttac atgggtgtca
      481 acttaggggc attattagca ccactaatcg ttggtacaat cggtcaagaa tataactatc
      541 atttagggtt ctctttagct gctattggga tgttctttgg attattacaa tacatcatcc
      601 aaggacgcaa gacattagac ggagtgggta caacaccgcc gaatcctttg acaactgaag
      661 aacgtaaagg attcatccgt aacttgatca tcgcacttgt tgttatcgct gcagtattcg
      721 gcggggcaca actaactggt catctgacaa ttgacttttt tgtcaataca atcagtgtat
      781 tggggattat cttaccgttg tattacttta tcaaaatgct gacttcaaaa gatgtaactg
      841 ctgaagaaaa accaagagta tgggcttata tcccattatt ccttgcagca atcattttct
      901 ggtcattaca agaacaaggt tcatctatct tagctttatt tgctagtgaa cgtacacaat
      961 ctacgctatt tggtgtaacg atccaagcaa gctggtatca atcattgaat ccaattttca
     1021 tctttattct gacaccagta ttcgtaacga tgtggacgaa acttggtgaa cgtcaaccat
     1081 ctacagtagt gaaattctct cttggacttt tatttgcagg attatcgtac ttgctattga
     1141 tgttgccggg tatgctttat ggtacaagtg gaaaagtaag tccattgtgg ttgttgggaa
     1201 gtttcttcat cgtggttatt gctgaattgt gtctttcacc agttggtttg tctgtgacaa
     1261 caaaattagc accacgagca tttgaatcac aaacaatcgc tatttggttc ttagctgatg
     1321 cttcttcaca agcaatcaat gcacaaattg cacgcttcta cacaccggga acagaatctg
     1381 cttacttcgg tattatcggt gcagtagcaa tcgtcggtgg tatcttgcta tttactgtga
     1441 aagaaccaat caaaaaacta atgggctcta ttcattaatc aatgaacata aaaacaggaa
     1501 ttgcttattt gcagcagttc ct
//
LOCUS       DAFGZE010000197         1515 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.203, whole genome shotgun sequence.
ACCESSION   DAFGZE010000197 DAFGZE010000000
VERSION     DAFGZE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.203"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..239
                     /locus_tag="LTU54_003120"
     CDS             <1..239
                     /locus_tag="LTU54_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBL8297749.1"
                     /translation="QILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEEQVAKLRE
                     LFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            259..1479
                     /locus_tag="LTU54_003121"
     CDS             259..1479
                     /locus_tag="LTU54_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBL8297750.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
BASE COUNT          525 a          273 c          306 g          411 t
ORIGIN      
        1 gccaaatatt ggattcacca gagttaaagg cacgaattat gccaagctca gaatttttat
       61 tgggtcattc actccaagcc tttgatgagc aaggacgact cattgatgaa gaacaagtcg
      121 caaaattacg tgaactattc aaagatttct tactctttgt cacaatcact agtcaattgc
      181 aacatgcgca ccaagcaaat aaacgtgaag ccgaaagctt ttcatgggaa acgatttaat
      241 cgaggggagc aaacaaagat gaaattaata ggtatcgtcg gatcagcagc tgaactttca
      301 tataaccgta aattattaac atttatccaa aaacaatatc atgaactatt taatttagaa
      361 atcattgaaa tcaaagattt acctctgttc aatcaagatg atgatcaaac aaatagtcct
      421 gctattcaac agctaaatcg caaaatctta atggctgatg gggtaattat tgctacgcca
      481 gaacacaatc atacggttcc agcaggattg aaaagtgcac ttgaatggct atcatttaaa
      541 atccatccac ttgaaaataa acctgtaatg gttgtgggtg cttcttacta cgatcaagga
      601 acttcacgcg cgcaattaca cttgcgccaa attttggacg caccaggcgt gaatgcatta
      661 gtgatgcccg gaaatgaatt tttattggga aaagcaaaag aagcatttga ccaagaagat
      721 cgcttgattg atccacgaac tgttgacttt ttaggcagta cattagcaag ttttgtaaaa
      781 ttcattaaag tcgtgtctac cttagcaggg cctaaaaagc ttccaccaga agatttgcat
      841 gcgacaggaa caattgctac gacaataccc gatgttgata tgtcagatga tcattggttg
      901 gaaaaagctt ctgaagcagt cggtgccgtt tctggaaata cttacgtcaa attaaatcgt
      961 gggttattga cagtggatca aatcaattac tttttagatt cgatgccgat ggaattaact
     1021 tttgcagata ataataacca atttttatat tataatttgc gccaagaagc gaaagagatg
     1081 ttagctgctc gtgtgcccgg tcaggtaggg aatccattag ccaactgtca cccagaaaaa
     1141 tcgtataaaa atgtagagtg ggtaattcaa caactgcgaa gcggagcaac agatgcggta
     1201 cgtgttcatg taccaaaaca agtgccagaa aaatatattg tgcataatta ccaagcaatg
     1261 catgatgaaa atggagagta tgctggaatc aatgaataca ttcttgattt caaaccaatc
     1321 attgagtggt atttacaaca aacgggtcaa caacttgttg gaaatgaaga tgcggtttca
     1381 agtgcatctg ttcatggtgg aaaaacagat gcagtaacca gcgccagcac acacgatgaa
     1441 actgcagtag atgcagtttc gagtgcttct gtaaaataaa aaggttctgt tgcaaagttt
     1501 taaataaaga ataaa
//
LOCUS       DAFGZE010000198         1492 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.205, whole genome shotgun sequence.
ACCESSION   DAFGZE010000198 DAFGZE010000000
VERSION     DAFGZE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.205"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(606..962)
                     /locus_tag="LTU54_003122"
     CDS             complement(606..962)
                     /locus_tag="LTU54_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HBL8297751.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(976..1386)
                     /gene="spx"
                     /locus_tag="LTU54_003123"
     CDS             complement(976..1386)
                     /gene="spx"
                     /locus_tag="LTU54_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL8297752.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
BASE COUNT          458 a          274 c          212 g          548 t
ORIGIN      
        1 tgtttgttgt cttgaattga atcaagacgc cctacacatt tggtttgtct tactttgttc
       61 agttttcaaa ggtctacttc atcaggagtg ttccccgatt cgtttttggc tgccgtagca
      121 acttttatat catatcagag agacaagtaa ttgtcaactc ttttttaaaa gtttttttga
      181 tttcgttttt cgaaatctta cagcaactca tttatcataa cttatcattt gttatttgtc
      241 aagaactttt ttgaagtttt tttcgataga atcaagcttc acaagtggtt cttttttaac
      301 aactcagtta tcttatcatg tcatttctaa catgtcaaca actattttat atgtttttta
      361 aaatactatt ttattgcttt gctacttaaa acaacagaaa tgaatatatc atgtaaattt
      421 acacatgtca atatattttt aactctatat ccccacctag atttgaattt tgaccacata
      481 aattgagcat gtctctatag tagaaaaata aagtcaaaaa aagaacacat aacacgcttg
      541 atgctaagtg ttcgactgtt acaactgtat gaaatgctca gtgacttgat taaaacagtt
      601 aaagtctatt tatttaagat aagctgttcc atcaactgct cattgtatcc aataaataga
      661 acttcatcga taataatcaa aggacgtctg aggagattgg tatgttgtgt gcataaaaaa
      721 atcagttgtt tttttcctac acaggaaagg tcaatcgcaa tgttgtgaaa agcctcagac
      781 caagtcgcaa atagctgttc tgcttgcact tcttcaaaac tgcataattc ttctgcttct
      841 tctgtactta gatcgcgttc ttctaagtca acaaattgat aaggcaattg atggtgttct
      901 aaccattgtt tcgccttcag acttgttaca cagtgcgctg atccatagac tttcacttcc
      961 acgaaggctc cctccctatg aatttaagat gttattgttt tgtaaataga tcagatttct
     1021 ggcaacacat cgaatttcac gagggacaaa tgaccggatc tcatcttcat taaatccgat
     1081 tagtattctt ttttcatcaa tcaaaatagg tcgtctcaat aatgaaggat attctttgaa
     1141 taagacgatc atttgcttta aagttaattc gttaaaatct atgcccagct tttcgtaaat
     1201 tttcgaccga gtggaaataa tatcttgaac tcctccttca gtcgctgcca agatcttttt
     1261 gatctcactt tctgttaacg gttcggcaaa aatattcttc tcttcaaatt caagttgatt
     1321 cgcttgcaac catgctcttg cttttctaca ggaagtacag ctaggcgaag tatatacttt
     1381 tatcattttc tcactccttt ttaattataa tccaattcta cagtaatttc ttttaaaata
     1441 ccaattatta gctaataaaa tataaaaaaa atcaaaataa ctgattattt ac
//
LOCUS       DAFGZE010000199         1490 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.206, whole genome shotgun sequence.
ACCESSION   DAFGZE010000199 DAFGZE010000000
VERSION     DAFGZE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.206"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            226..1101
                     /locus_tag="LTU54_003124"
                     /note="aph(2'')-Ia"
     CDS             226..1101
                     /locus_tag="LTU54_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL8297753.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1231..>1490)
                     /locus_tag="LTU54_003125"
     CDS             complement(1231..>1490)
                     /locus_tag="LTU54_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297754.1"
                     /translation="KYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEVRR
                     REKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          597 a          139 c          252 g          502 t
ORIGIN      
        1 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
       61 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      121 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      181 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      241 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      301 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      361 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      421 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      481 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      541 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      601 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
      661 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
      721 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
      781 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
      841 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
      901 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
      961 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1021 gtttatggaa ttaaaaatat taaacaggaa tttatcgaaa atggtagaaa agaaatttat
     1081 aaaaggactt ataaagattg attatataat atatgaaaag ctattataaa agacattagt
     1141 attaaatagt ttaaaaaaat gaaaaataat aaaggaagtg agtcaattcc agactcctgt
     1201 gtaaaatgct atacaatgtt tttaccattt ctacttatca aaattgatgt attttcttga
     1261 agaataaatc cattcatcat gtaggtccat aagaacggct ccaattaagc gattggctga
     1321 tgtttgattg gggaagatgc gaataatctt ttctcttctg cgtacttctt gattcagtcg
     1381 ttcaattaga ttggtactct ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa
     1441 ggcgtcttcg aatccatcat ccaatgatgc gcaagctttt gaatattttg
//
LOCUS       DAFGZE010000200         1484 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.207, whole genome shotgun sequence.
ACCESSION   DAFGZE010000200 DAFGZE010000000
VERSION     DAFGZE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.207"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..396)
                     /locus_tag="LTU54_003126"
     CDS             complement(<1..396)
                     /locus_tag="LTU54_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBL8297755.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFK"
     gene            complement(378..1367)
                     /locus_tag="LTU54_003127"
     CDS             complement(378..1367)
                     /locus_tag="LTU54_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBL8297756.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
BASE COUNT          485 a          275 c          189 g          535 t
ORIGIN      
        1 cttaaaatct atttcttcta tagatttacc agctgactgg gccattaata actccagtaa
       61 aagaggtaaa ttaatcccag caataatttg aattctattg ttgttcaact gtttaataga
      121 aacattatta ggtgttcctc caattaaatc cgttaaaata attagaggtt gcaaatgatg
      181 ctgactaatt atttctttca tactctcctc aaaatcatct ggactagtat tttctttaaa
      241 tggaacagca ctaatacctg tggcgtcatt cattaaaatt aattttgttg cctttaaata
      301 accctcacaa agggtagaat gtgttgtaat cacaatgtga aataattgat ttgccatctt
      361 ttgcaacctc ctattaatta atatttcatt tgccacatat agcgtctaac atctaaggaa
      421 tggcctcttt caaaagcaat cgcttctacc atttggcgaa taacaacgcc actaagtaca
      481 gctgcaatat attctttcgc tacactatct atagattcta agtcaaaatc agcttggtct
      541 aaaaccatta atttattact gaatttttta gtgaagtctt ctacccgttg atctaaaaat
      601 cttgtatctc caatactttt cactaagatg aatggtacat cataatcagt gatttcaaaa
      661 ggaccatgga aaaattcccc agaatgaata gcattagaat taatccattg catttccatg
      721 aaccagcaag ctgctgtgga gtaaatttct ccgtaattga taccgctacc aattgtgtaa
      781 ataattttat ctcgtttttg gtttttaccc cattctttag cagttatttc aaatttgctc
      841 ttttcagcaa taacaagatc atttaactta tcaaattcat tcaagcaagt ttcccatttt
      901 ttagagccat ctaaaacaag taaaatatta aataataaac cataaagaat tcctttattt
      961 aaatcactag catcaacatc tttaccccaa tcatagtgaa caggatattc agctgcttcc
     1021 catagaggag atccttcttc attagataaa caaatagtca ttgctccaaa ttctcttgct
     1081 ttttttgttg cttctacagt ttctggtgtt gtgccagaat gggaacaagt gataactaaa
     1141 gaattttcag ataatccttt tggtatagca tagttaaatt cgttagcagt ataaacatga
     1201 caaggtacag ttaattcttt atcaaataga tattgaccag ccattaaaac ggcttgtgaa
     1261 cccccacatg ctgtaaaata tacatgatca attgtctttt cctttaacgc tgccattgct
     1321 gtttcaatct gttctttgtg tagttgtctc atttctttat ccctcactct aataagtatt
     1381 ataaagtcat ataactttac aatacctatt atctatgaaa ttgctttatt ttgcaatact
     1441 atttttcaaa aaaatttttt tataacaatt ttatataata aaat
//
LOCUS       DAFGZE010000201         1460 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.209, whole genome shotgun sequence.
ACCESSION   DAFGZE010000201 DAFGZE010000000
VERSION     DAFGZE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.209"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="LTU54_003128"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="LTU54_003128"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HBL8297757.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          508 a          188 c          242 g          522 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgatgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatattata gattacaaat agtatcgtat
     1441 ttcgcaatct taaaattatc
//
LOCUS       DAFGZE010000202         1457 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.210, whole genome shotgun sequence.
ACCESSION   DAFGZE010000202 DAFGZE010000000
VERSION     DAFGZE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.210"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            108..1040
                     /locus_tag="LTU54_003129"
     CDS             108..1040
                     /locus_tag="LTU54_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HBL8297758.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1457
                     /locus_tag="LTU54_003130"
     CDS             1112..>1457
                     /locus_tag="LTU54_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297759.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          641 a          169 c          227 g          420 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caagata
//
LOCUS       DAFGZE010000203         1424 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.211, whole genome shotgun sequence.
ACCESSION   DAFGZE010000203 DAFGZE010000000
VERSION     DAFGZE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.211"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(150..1337)
                     /locus_tag="LTU54_003131"
     CDS             complement(150..1337)
                     /locus_tag="LTU54_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HBL8297760.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DAFGZE010000204         1422 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.212, whole genome shotgun sequence.
ACCESSION   DAFGZE010000204 DAFGZE010000000
VERSION     DAFGZE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.212"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..535
                     /locus_tag="LTU54_003132"
     CDS             <1..535
                     /locus_tag="LTU54_003132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297761.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKIRPFIF"
     gene            562..>1422
                     /locus_tag="LTU54_003133"
     CDS             562..>1422
                     /locus_tag="LTU54_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297762.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKK"
BASE COUNT          590 a          223 c          317 g          292 t
ORIGIN      
        1 catggcagaa gaaaaagaac cactagacaa tagtcgtttg ggaaaatcca aaagaaaatt
       61 agtacgattg caaaatgaat taaacgaaca aattgaaaag atgtttgaac accagagaaa
      121 gaccaacggt caaccaatga acgataagag aaatgggcat tcttggttta ggcaacaaga
      181 gcgaatcgaa aataaagtgc atagtctgag agaagagatc aagcaacaag aaaaacaagt
      241 agaaaaactt gaaagacaag aagagataaa ggaaatgggc tataacaagt acggcggact
      301 ggatatgacg atcgagaata ttccaagaat caaagaagaa atcgaacgct ttgaaaaagg
      361 tgaatcgact ttttcagccg ctacgatccg aaaataccaa cggaaattgg aaacattaga
      421 acagctgaaa gaaagatcgg aaaaaggaaa agaaaacctt cttcctgaag tacaagcaat
      481 cattgacagc ggacgagtga cgcaatggaa aaaaatccga ccatttattt tctgaaaggc
      541 tatcgaaaag tcgctttaga actgaatgaa aacggggaat ttgaggaatc taaaaaatac
      601 caagccacgt cggaagagga aaaagcagct gttcgtgaat tacttgaaga ggctgtacca
      661 actgattttt tgaaggagaa agaaaacgat catttttatc gagtagaatt caacgaaaat
      721 gatccagaaa aaggattgaa aagttatggt ggggagatcg taacattcga attgatcgat
      781 caactcaaaa cgttggataa ccaatacgcc gcagaagcag gttattataa attctatttt
      841 gaagaagtga agaacggaga agtagtcgat cagatccgaa tggatctagg ggatggcgtc
      901 aacagtaatc aacccatcta tttagagtta gcaaaattgt gcgaacaagt acctgagaag
      961 gaacaaaaac aatcggtcaa aaatgaacct gtttcgaaag aacaaatgca gtcgaaagaa
     1021 acaaaaacaa aacaaaaggg ctatcaaaaa cgaacggcag aagagattaa acaagaagtt
     1081 aaatcccttt cgcaacaggc actggatgcg gtgaaaaagc atactcattc tcctgaagac
     1141 gtcaaagaac ttttgaactt tatgtctcgt tttccagaac gatcctttag aaatcaatta
     1201 ttgattgagg aacaatttcc aggtgcaacg gcttgtttag gtcgtgccgc gttgaaaaaa
     1261 gaaggaatct atatcaaaaa aggggaacaa gggaacaaga tctttgtcag aaaaacggtc
     1321 aaaggatttt atgagaaagg acgtggcttt gttcgagaaa tagaagctac accagaagat
     1381 aaaaaacgaa tcgaaagtgg tcaaatcgaa gtgatcaaaa aa
//
LOCUS       DAFGZE010000205         1414 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.213, whole genome shotgun sequence.
ACCESSION   DAFGZE010000205 DAFGZE010000000
VERSION     DAFGZE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.213"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..566
                     /locus_tag="LTU54_003134"
     CDS             <1..566
                     /locus_tag="LTU54_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297763.1"
                     /translation="YLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTV
                     EGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYE
                     YYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYNFYKVEASL
                     RYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            610..879
                     /locus_tag="LTU54_003135"
     CDS             610..879
                     /locus_tag="LTU54_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297764.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1155..>1414
                     /locus_tag="LTU54_003136"
     CDS             1155..>1414
                     /locus_tag="LTU54_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297765.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIA"
BASE COUNT          538 a          183 c          275 g          418 t
ORIGIN      
        1 gttacttact cgtccaattt atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt
       61 tattggactt acttatctta ggcagtatgt taacgactct aacagatcgg ttcgaacaat
      121 ctgaaaaaac ggtagagggg ataaagaact tcttaacgac gattgatcaa tttataaatg
      181 gatatatgta tttgtttgaa gtaggcttta ttctcacttt gtttggttgt gtatttataa
      241 aactcattaa aagatcaatc gaaaaagaaa tggttattga tctaattccg acgctgagcg
      301 gctatgagta ttacgttgat tttggaaagt atgaggagtt gatccatacg gatatcgttc
      361 acacgagtat ccatgaagta accgttaagg gcatcagatc tactttaaaa gatggacagg
      421 agacgaaaac aatcgaccga agaaaagagg tctacaattt ttataaagtt gaagcaagtc
      481 tacgatatgg cattgggaaa ttgaggttag atgggagtaa tattcaacaa aacaaaccat
      541 atgttttagg aaaagtggtg atttagaact tcctatgaaa gatgaataaa taaggagaat
      601 cttttgaaaa tgattcttct ttacagaatt gtgcttatag ttggcacgga gttaaaaatc
      661 ttaaagaagg agcagttatt caaagtgaaa gaacatcata atcagttact atgtattaaa
      721 acgggtacga tgaacattca ataccgaaca gaatatttag aattaacgaa tcaaactgat
      781 tttttgtcga ttcgagagca acataagcaa gaaaaattaa tggctactta tagccaattg
      841 attgatgagt gggaacaatt caaggaatct aaaacataaa attccattta aacaccttat
      901 tttttaaaga gcataaggaa acgtgtaccc actttcaaaa taaagtgggt agataggctg
      961 tgcctatcac gtttctgaaa aaaataaatg accccaaaat tttcaattta tgtgtaggtt
     1021 tatgggtagc aaaaatatga atgtgggcag caaaaagttc atttgtgggt agtaaaattc
     1081 aaatgtgtgt agcaatgtgt gtagctacac acattgctac acacgctata acatatagtg
     1141 aaaaggagat atttatggaa gtaaaaataa gagatattga tgaaaaaacg attcaaagaa
     1201 ttgaccgaag atgtattgaa ttaagtgaac gcaccgggac taaatggagc cgaaatcgat
     1261 acctaaaagt tttgattgaa aatgattttg accatgaact tatggatcat agaaaagatc
     1321 aatttgatcg gttgttagaa aaatttgtgg atattcaaac ttataatacg aaaattttag
     1381 aagaatatat tgcaacaaat aatcaattaa tcgc
//
LOCUS       DAFGZE010000206         1387 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.214, whole genome shotgun sequence.
ACCESSION   DAFGZE010000206 DAFGZE010000000
VERSION     DAFGZE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.214"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            108..401
                     /locus_tag="LTU54_003137"
     CDS             108..401
                     /locus_tag="LTU54_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297766.1"
                     /translation="MGILQRSEIVKELFYHKFSYEIRLDDTYEHYRIVFFVYNSIEDA
                     TATTALTFGFTKNGTINSDKTRQAATESDGICKKVCNGEENYWIGEEKLNEIY"
     gene            388..840
                     /locus_tag="LTU54_003138"
     CDS             388..840
                     /locus_tag="LTU54_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297767.1"
                     /translation="MKSINDLLKKHPDLYKKLNSEQRNLSKQLLKIRLKKQKNVTEFS
                     KMLGISEKKYLEFEFGDMHIPVAEYNKLLKQVENLSYKDSMNFTIKVAGGPIKYDRIE
                     NKPYIRNKSYSVFKSRKRKEVSSNMVYASVNYQDTSRYIVEKEVYVHG"
     gene            833..1231
                     /locus_tag="LTU54_003139"
     CDS             833..1231
                     /locus_tag="LTU54_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297768.1"
                     /translation="MDKGNSVTFALENISLNKIHFELTDNFSYGDLEFNIELGYKEDE
                     AEYLEKKLVSLRAHIVGGMEGAPVDILLFSVIRVSTNEQPYKKLVEESMGELAKPILN
                     KASNLISNLYSEVGVMPTVIDLYQLYSQQK"
BASE COUNT          541 a          169 c          240 g          437 t
ORIGIN      
        1 tccttctatt aaaatatctt gaaatttact ctgttaaaaa aaataaatta attggtgaaa
       61 atgattctcc aataaaaaca cctattgagc taagcttaag aaatcggatg ggaatactcc
      121 aaagatcgga aatagtaaaa gaactttttt atcataaatt tagttatgaa ataagactag
      181 atgatactta tgagcattat agaattgttt ttttcgtata caattctata gaagatgcta
      241 ctgcgactac tgcattaaca ttcggtttta cgaaaaatgg aacaatcaat tcggataaaa
      301 cccgtcaagc tgctacagaa agtgatggta tatgtaagaa agtatgcaat ggggaagaga
      361 attactggat aggggaggaa aagttgaatg aaatctatta atgatttatt aaaaaaacat
      421 cctgatttat ataaaaaatt aaattctgaa caaagaaacc tgtccaaaca attacttaaa
      481 ataagattaa aaaaacaaaa aaacgtgaca gaattctcaa aaatgttagg aataagtgaa
      541 aaaaaatatc ttgaatttga gttcggagat atgcatattc ctgttgcaga gtacaataaa
      601 cttttgaaac aagttgaaaa tttgtcctat aaagattcaa tgaatttcac tattaaggtt
      661 gctggcggcc ctattaaata tgaccgtatt gaaaataaac cttatattcg gaataaaagt
      721 tactcagtat ttaaaagtag aaagagaaaa gaagtatcgt caaatatggt ctatgctagt
      781 gttaattacc aagatacgag ccgctatatt gtagaaaaag aggtatatgt acatggataa
      841 aggaaatagt gttacttttg cacttgaaaa tattagtttg aataaaattc attttgaatt
      901 aactgataat tttagttatg gagatttaga gtttaatatt gaacttggat ataaagaaga
      961 cgaagcagag tatctagaaa aaaaacttgt ttctttacga gctcacattg taggggggat
     1021 ggaaggagca ccggtagata ttttactctt ttcagttatt cgtgtttcta caaatgaaca
     1081 gccatataaa aaattagtag aagaatctat gggagagcta gctaaaccta tcctgaataa
     1141 agcttctaat ttgatttcta atttatatag cgaagtagga gttatgccta cagttattga
     1201 tttatatcag ctatatagtc aacaaaaata aaatacaagt aatagagtta tctatttaaa
     1261 tttgcaagac aaagacctca accagtcatc cttggttgag gcttttttgc tctgtaatta
     1321 actcgtatag atcctgcaag atagtttgtt tctgaataaa tgattgatat gaagtattta
     1381 gatgact
//
LOCUS       DAFGZE010000207         1378 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.215, whole genome shotgun sequence.
ACCESSION   DAFGZE010000207 DAFGZE010000000
VERSION     DAFGZE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.215"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..254)
                     /locus_tag="LTU54_003140"
     CDS             complement(<1..254)
                     /locus_tag="LTU54_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HBL8297769.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
     gene            complement(254..517)
                     /locus_tag="LTU54_003141"
     CDS             complement(254..517)
                     /locus_tag="LTU54_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HBL8297770.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            751..1378
                     /locus_tag="LTU54_003142"
                     /pseudo
     CDS             751..1378
                     /locus_tag="LTU54_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
BASE COUNT          447 a          265 c          207 g          459 t
ORIGIN      
        1 gtccttgctg aataaatttc aaccacgtgt tcatcttcat ctaccttata aacaacccgg
       61 tgttgtctat tcaatcttct agaataccta ccttcatgtg taggttttaa cttctcaaat
      121 gattgggtag ggagataggg atcttccttt aaagtctgaa taaccttctc aaattgaggt
      181 ttcaaattag actgtttgat tttctttaaa tcagctttag ccgagttttt aatcatcaca
      241 gaatagttat tcattataag ttatcccaat caatttcatc aacattagta aatccactat
      301 catctttttc tcgcttacga acaacaccca aagtgcctat ttgctcaagt aatagcgttt
      361 ctttgattgc tttccaatca tccaaaccaa taagcaccgc attattttca ctgcgctcac
      421 taatgatttc aatttccgta tgattctcat ttacatcttt caacaactta taaaaatttt
      481 tccgtgctga gctaggattt aagactgttt ttgccatatc tactccccct ctaatttata
      541 tctccattct aacgtactta attaagtacg taaattcgat tgttttgttt aaattggagt
      601 accaaaacag cattgtaaag tgttatccgt ttcctttata cccgtggtgc tagttaattt
      661 tttcctagag gaaaatgtct aaacatgttg gtaaatcaac aaaaaaacgc atagagtagt
      721 ctcaaaactc ccaagaaatg aggactacat atgcgttcat caaaatatac tgaacattat
      781 acagatactt ttataacatt caaggaaatt atgagaactg tttctaacat ataccacaat
      841 tgtgtaccag ataagattaa aaaccgaaga aatactgatc aactgaagta acgcgataca
      901 gtgattattg cttgtgttat atggggcata attaatggct atactagcca aagagccacg
      961 tatagagcgg tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc
     1021 actcgcttaa gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag
     1081 aagctcacca aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa
     1141 ccagttagaa atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca
     1201 acaaaaaaat cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt
     1261 ccgattactt actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta
     1321 tcggaagaag cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAFGZE010000208         1374 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.216, whole genome shotgun sequence.
ACCESSION   DAFGZE010000208 DAFGZE010000000
VERSION     DAFGZE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.216"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            39..1340
                     /locus_tag="LTU54_003143"
     CDS             39..1340
                     /locus_tag="LTU54_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HBL8297771.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DAFGZE010000209         1362 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.217, whole genome shotgun sequence.
ACCESSION   DAFGZE010000209 DAFGZE010000000
VERSION     DAFGZE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.217"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            82..>1362
                     /locus_tag="LTU54_003144"
     CDS             82..>1362
                     /locus_tag="LTU54_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HBL8297772.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          464 a          262 c          257 g          379 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat ta
//
LOCUS       DAFGZE010000210         1332 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.219, whole genome shotgun sequence.
ACCESSION   DAFGZE010000210 DAFGZE010000000
VERSION     DAFGZE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.219"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..228
                     /locus_tag="LTU54_003145"
     CDS             <1..228
                     /locus_tag="LTU54_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297773.1"
                     /translation="YDGLNMVYQEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGF
                     TRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
     gene            244..888
                     /locus_tag="LTU54_003146"
     CDS             244..888
                     /locus_tag="LTU54_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297774.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            899..>1332
                     /locus_tag="LTU54_003147"
     CDS             899..>1332
                     /locus_tag="LTU54_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297775.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDP"
BASE COUNT          517 a          207 c          286 g          322 t
ORIGIN      
        1 tacgatggct tgaatatggt gtatcaagaa tttgcaaata gtacaggaaa ttctcgagtg
       61 aaagtaggcg atcaagtgaa agttggtcaa gtaattggga tacgagatac agcacacttg
      121 catttaggat ttactcgaat ggattggcgt caagcacaag gacatgcatt cattgatgat
      181 ggtacatgga tcgatccgtt accatttttg aatagcagta aaaaatagat caggagaaaa
      241 aatatggaaa ataaaacgtt aaaaatcgtc ttaacagctg caattagcgt tagtttaatc
      301 ggcaatattg cgttgttttg gcagctgaaa gaggaacaag cagcaaatac ctataaaatt
      361 gaagaagtgg ctaaaaacgc gtctgagaag ctcaaaaaaa attcctctga ggcacaggcg
      421 aagatcgaca agttaaatca aaccatcaat gagaaaaacc aagaaatcga tcagctgaag
      481 caacaagatg gcacaaaagc aaaagaggaa gtaactgaag cacaaagaaa gacggcagaa
      541 gagttcggtc aattggccat tgataaatca ttaagcagaa atgaattaac ggaaaaacta
      601 aaagatgtcg caacccaaga agtgatcgac aaattaagtc ctacagacga tgatcaccaa
      661 catgatgatt acggaagtta ctcctttaca ttaggtgacg tgcaaactta cgctcaaaca
      721 agcagtgtaa aagaggaaca aaattttgtc gtatttgttg attatacaat cggcaatccg
      781 caattcaaag atgtcaaacc acaaaaaata aaaggtggat taactgtcac tgaaaaacaa
      841 gtagatggtc aatggaaagt catagacttt agttatttca ctcgttagga ggaagaaaat
      901 gagtaagaaa aaaggagaaa gacaacgttt caaaaaacga aaaagttatc ttagatggct
      961 gggaatcttt atgattctcg gctttttgat tcttgctagt ccgatttata cgaatcagaa
     1021 ggtagatata ccagatgagg taagcacgaa cgccttttta aacacgaaag agctgacaat
     1081 ggtttcaaaa gaaaaaggag cgaaccagca attagttttt gaatttgcga tcgatgatga
     1141 agatcaaagc ttgttaaaag aactggctaa tcttaattat cgcgtagaag taaagacagc
     1201 taaaggggat tatcaagcaa tacgagccaa agtgattaag gtgtctgatg attattttgc
     1261 agtgaagcta acgcatgtac cagaagaata tatcgccatg cgtttaacca ttattcctga
     1321 aaaaattgat cc
//
LOCUS       DAFGZE010000211         1312 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.220, whole genome shotgun sequence.
ACCESSION   DAFGZE010000211 DAFGZE010000000
VERSION     DAFGZE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.220"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(156..1297)
                     /locus_tag="LTU54_003148"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="LTU54_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8297776.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DAFGZE010000212         1305 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.221, whole genome shotgun sequence.
ACCESSION   DAFGZE010000212 DAFGZE010000000
VERSION     DAFGZE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.221"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            233..487
                     /locus_tag="LTU54_003149"
     CDS             233..487
                     /locus_tag="LTU54_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBL8297777.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            488..805
                     /locus_tag="LTU54_003150"
     CDS             488..805
                     /locus_tag="LTU54_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBL8297778.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          454 a          198 c          257 g          396 t
ORIGIN      
        1 acctatagag gatgggcgac ttctgcctaa ttctgttaaa tggatgttaa cggaagtgta
       61 tggtgagtag ttcactcgta ttttgatatc gtttgtatat gttttcagcg ttctaagtgg
      121 cttattttgt ccaataataa tatggagtgg aatcccttaa gaaacaccgt tggcaattgt
      181 tgttataaca tgttataatt tattcatcaa aaactaaaag gagtgtgata acatgggaaa
      241 agaactaaag gttagaaaga tcggcaatag cgtgggagtg atccttccaa gctctcttgg
      301 attaaaaagc ggagacatta tccaagccaa acaagaaggc aacttattca tattagatac
      361 tactcaaata gcaaaagaac acgatcgcaa attgattgaa gaaagtttcc aagactttga
      421 gaaaggttta acagtttcag aaattgaaat ggtaaaagct ttcggaaaat acggctggag
      481 tgaatagatg aaagagtaca cgctagttta ttctaaaagt tttcagacca gccttgaaac
      541 gataatagaa gaatgggaaa atgagcttgc tctttctcca gccagcataa aaagatttgt
      601 aggtgcgatc cagaaggctt tagaactaac caaaaatttt ccaactatgc atgaggaagt
      661 ttccgaaatc tatggaatgg atatcccaac atatcggatc gtgatcggca aaagctatgc
      721 cttattttat cggatcgacc atgaaaaaaa agagatactt gtaggacgta ttttccaatc
      781 taaacaaatg aaactaggct tttaacaata attaggaaaa agtcgctcat cctctacagg
      841 tgggtgatta atgccgctcg atatggttgt taaccgtcgg ttactcgaag attgatatat
      901 aagacttagt acctttttat tttgctccat gttatggccg aaggtctaaa gatacaatca
      961 ttagtctaga gaaaaattaa gaaccgtagg aactacgggg atcgcttggt gaataagaga
     1021 cactgctatt agtgaaaaaa taagatatca agtacgctct attcccaaaa atttcgtgac
     1081 tttactcatg agaggttcaa gtagtatatt tttaatcaaa attgacgtaa cttttaataa
     1141 atactaccta aataaggtag tatttatttt tgtgttttaa aagaggaagt ccaatatatc
     1201 tatgggaaac ttcttaaata ttatgttggt acatgaaaat atacatccct gctagactat
     1261 ttacaacatt ttactttgta cgtattcttg ttttaaaagg aatgg
//
LOCUS       DAFGZE010000213         1294 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.222, whole genome shotgun sequence.
ACCESSION   DAFGZE010000213 DAFGZE010000000
VERSION     DAFGZE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.222"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..1244
                     /locus_tag="LTU54_003151"
     CDS             <1..1244
                     /locus_tag="LTU54_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBL8297779.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DAFGZE010000214         1288 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.223, whole genome shotgun sequence.
ACCESSION   DAFGZE010000214 DAFGZE010000000
VERSION     DAFGZE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.223"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(61..279)
                     /locus_tag="LTU54_003152"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="LTU54_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="LTU54_003153"
     CDS             complement(545..739)
                     /locus_tag="LTU54_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297780.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="LTU54_003154"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="LTU54_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          456 a          217 c          175 g          440 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaa
//
LOCUS       DAFGZE010000215         1273 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.224, whole genome shotgun sequence.
ACCESSION   DAFGZE010000215 DAFGZE010000000
VERSION     DAFGZE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.224"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..234
                     /locus_tag="LTU54_003155"
     CDS             <1..234
                     /locus_tag="LTU54_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL8297781.1"
                     /translation="QTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDI
                     DSEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            239..1186
                     /locus_tag="LTU54_003156"
     CDS             239..1186
                     /locus_tag="LTU54_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBL8297782.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          417 a          183 c          265 g          408 t
ORIGIN      
        1 cagacacctg aagtttttga aatgaatgcc tcaatgtatt cttttaaacc tgaatttcta
       61 aaaaaaagta attatatttt tgatgggcaa tgtgggataa taaaaatgag agattatcta
      121 attttagata ttgatagtga agaagatttt aaatggttag aatttttgtt tcctaaattt
      181 ttagaagatt cttcggagct tcaagatatc tataattatt tatatcggag gtaaaaggat
      241 gagctgttat cttatcaccg gtggggctgg cttcattggt tctaccttag ccaatcattt
      301 aggagaagaa catgtggtaa tcgtcgtcga tgacttatca atgggaaaag tagaaaatct
      361 tgatatgacg aaaaatatca cttttattga gggagatgtc gctgagaaat ccttgatgga
      421 agagatcatg aaagctaata aatttgatta tatctttcat ttagcagcag ttgctagtgt
      481 ggcagattca gttgcccgac cagtagaaac acatcgtgtg aattttgaaa gtgtattaat
      541 gattttagaa ttgatccgca agtatcaacc tgatttaaaa cggatcgtgt ttagttcttc
      601 agcggctgtt tatggggatg aaccgacttt accaaaaaaa gaagagtccg tgattcgccc
      661 attgacgcct tatgcggttg ataaattcgc tgcagaacaa tatgtgctag attattgtca
      721 tttatatgat gtacccggta gcgcagttcg ttttttcaat gtctatggtc caaatcaaaa
      781 tccgaattct ccatacagtg gtgtgatttc tattctcgta gatcgctata agaaacaaat
      841 cgtaggagaa gcaacttcat ttacattatt tggtgatgga agccagtcta gagactttgt
      901 ttatatagat gatgtaatcc aagcattact tttagttgcg aatgaggaag ctgcactggg
      961 gaaacaattc aatgttggaa cgggtaaagc aaccacactt ttggaattga ttcatgcaat
     1021 caatgaaatt ctcggtgtag aattgacact tgaatacaaa gaagagcgtt cgggtgatat
     1081 ccatgactct ctagcagata ttactaagat ccagtcaatt ggctatcaac cgaaatatgg
     1141 tattttaagt ggaatgacta attatttgaa aactgaaatc aattaaaaca tactaagata
     1201 agattagagg gtaaattcag gctctttgtc aataaggact gatgagtttt gtaaaatcaa
     1261 atctgggata gaa
//
LOCUS       DAFGZE010000216         1251 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.225, whole genome shotgun sequence.
ACCESSION   DAFGZE010000216 DAFGZE010000000
VERSION     DAFGZE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.225"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(52..1074)
                     /locus_tag="LTU54_003157"
     CDS             complement(52..1074)
                     /locus_tag="LTU54_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBL8297783.1"
                     /translation="MIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFIPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          311 a          267 c          201 g          472 t
ORIGIN      
        1 ttcgcttgtg acggcttttc tgctaaaata acggttttca tcatgacgat ttcactccct
       61 ttcttgttgt ctgccttgtt gaaacaacat ggcacgtttt tgctttctga tttcttgttg
      121 ctggatctca tgcttcaatt gatcgttcca gtcctttttt acttcaggta taaattcatg
      181 ttcatgattt ttatagcctt tgtagtattg ttctgcttta taagagaaaa gaatctgcgc
      241 attaatatcc cctttttctt gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc
      301 acaaactttt aattcttttg cgacttctct acaaatatct ttcaaattaa tggcttctat
      361 tgaatcgtta acagaaagct gctggccaaa aaacgcattt actttgtgag cggaaaccag
      421 ttggttcgtt aattgcagat tgttttctgt cttgtgtatg gccataatag cttctggtgt
      481 gaccttttct tcttttgcga cttcttcata aatgattttg tattcttttg gcacttgcca
      541 atcattagcg attcctcgtt cacaacgtac tttctgattc gtaaatggat cgactgcgcc
      601 cattaattgt tctttttcgt agaaaatatg tcctgcacgg tcattatcca cacaaatctc
      661 gatggattga ggaaaggctt gtttctttct caattcagat actgcctcac caaaataatg
      721 actgatcaca tggtgtttta acccttccat agagactaac cacgtatcgt ttaactgatc
      781 acgattcagt gcagcatagc taagtaaatc aatactactc tcaaaaaatt ttaactgctt
      841 agggtcaccg atctttaggt taaagccatg attcgctgtc gaatttttat ctatatgctt
      901 atatgtgcct ctctcaccaa aacgttcata atcaatgaac gttccttgat aactcgcccc
      961 tactgtctct tttgtctgtg gatctttcca taaaaaggcg accgcatgat ctaaacgatc
     1021 gtctcgaact aagaaatttt gccgttcata ttttggcttt agttcggcaa tcaacccttc
     1081 tttttctgca taatcaatca gccactctgg tagttttcgt tcttccttca gataattcct
     1141 tcctttttct gtgagtggtt gataatcaac ggggtgatct ttgtactttt ctgtttcaaa
     1201 atgaagattt tccactcgtt ctgttttgtc ttcattcttt agaataaaat c
//
LOCUS       DAFGZE010000217         1247 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.226, whole genome shotgun sequence.
ACCESSION   DAFGZE010000217 DAFGZE010000000
VERSION     DAFGZE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.226"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            15..488
                     /locus_tag="LTU54_003158"
     CDS             15..488
                     /locus_tag="LTU54_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBL8297784.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            497..724
                     /locus_tag="LTU54_003159"
     CDS             497..724
                     /locus_tag="LTU54_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297785.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          394 a          196 c          209 g          448 t
ORIGIN      
        1 aaagagggat aaaaatgaaa tttaaaacat tgctattaaa atttgtcgtt ttaggtataa
       61 gtgccgtatt tattttgttc ggcgtacttt tttatactca gatcgctaca agcgaaaaag
      121 gattcaattt tgattggcag accacattat ttgtttctgt catttacttt gcccttctcc
      181 tagggatcgt tattgcttat ttcttattga aactattgaa aaaaatcgaa gataaccaag
      241 ctttttccac tcaaagctta aacatactaa aaaaaatcga acgaaccatt ttgtttattg
      301 gtttcgcctt ttttggctta cttcctaaat ttatgaaagt agcggattta gacgatgctc
      361 caggtttgat gttggtagga atcgctgtcg tattcattcc ttttgcagta tacacattaa
      421 caagcgtgct ggaaaaactg cttgaacatg cgattttact taagacagat catgatttga
      481 cagtgtgagg tgcgacatgg caattatcgt taatttagat gtagtattag ctcaaaaaaa
      541 aatgagtgtg actgaattga gtgagaaagt gggcattacg atggcaaaca tctctattct
      601 aaaaaatgga aaagcaaaag ccatccgttt ttccactctc gaaaaaatct gccaagtttt
      661 ggagtgtcaa ccaggtgatt tgttgaccta taaaaatgac tcgaatacta taccagaaga
      721 ataaaataaa gtagaacaaa cagtaaataa aaaataatct gaatgataga tgtgtgagac
      781 ttttagaagt tatttacgtc tattattcag attttttatt tcgattgaat accgttttat
      841 ctataaataa gatatgtact gactcataca aagggggcct tttcttctcc atgtactatc
      901 acttttcctg atacaactgg tttcctgtta cgttgtagtt ggatctgctt ttagtataat
      961 actttattca ttccaccaga ctgatagcag tttgagcagt ctaaataatc atgtagtatt
     1021 acaggccatt ctgtttcata ccttgaagtg cttaaaactt cagctaatgg cccattgtgt
     1081 taactcttac tccattcttt tttagtaagc tcccatatat actgtaaagc cttctatata
     1141 ttatgtcatg ttgcttttta acatttacta tgtagttttg ataaaatgat ttgaataaca
     1201 gtgatggaca attctttttt atttttaaca ttattttgat aataatg
//
LOCUS       DAFGZE010000218         1246 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.227, whole genome shotgun sequence.
ACCESSION   DAFGZE010000218 DAFGZE010000000
VERSION     DAFGZE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.227"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            200..742
                     /locus_tag="LTU54_003160"
     CDS             200..742
                     /locus_tag="LTU54_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297786.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="LTU54_003161"
     CDS             898..>1246
                     /locus_tag="LTU54_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBL8297787.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DAFGZE010000219         1218 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.228, whole genome shotgun sequence.
ACCESSION   DAFGZE010000219 DAFGZE010000000
VERSION     DAFGZE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.228"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            61..915
                     /locus_tag="LTU54_003162"
     CDS             61..915
                     /locus_tag="LTU54_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBL8297788.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
BASE COUNT          449 a          166 c          213 g          390 t
ORIGIN      
        1 atgtaataat ataaacataa taaaacaaaa attatccaaa tctaacatag gagggtggat
       61 atggaaaatt ctatatattt agtaacagaa cctaacactc agctgaaaga ttgttacttt
      121 aatttttgtg ggtattcaaa aacagaacct agacatagtt ttggaccagc tattcgagaa
      181 caatacttaa tacacatagt gctagaaggt gaaggctatt attcaattaa tgatcaaaaa
      241 tattttctga agaaaggcca aggatttgtt atcccaccag gagtatctac tttttatcaa
      301 gcagatgaaa aaaatccgtg gtcatatgta tggttgggtt taagtggcaa tcttgtagaa
      361 tactatctaa aagaaattgg tgttggaaag aatcatctta cctttaaagt cgaaaattta
      421 aattcattta aaggattaat ttttgaaact ttatcgtatg atcaggacaa tctagtcagt
      481 gaattgtcca gacaaaaaca agtttataaa tttttagaac tcctcttaaa ttcagctagt
      541 tatctgaacg aagggataat tactaaaaag atgaatcctt ttgttcgtca aacaattgaa
      601 ataatacgtg agaatgcgta tcaaaaatta tctgttaaag atattgctgt cagtctatcg
      661 atacacgaaa attatttatc acgattgttt aagaatgata tgggaatatc aataaaagag
      721 tatattagtg aaatcaaaat ttctattgca aacgatctgt tatcatcaac tgaatattca
      781 atccagcaaa tcagtgacaa gataggattc tcaacagtac aatctttttc taaagccttt
      841 aagaaagcaa atggtgtttc accgggagta tatcgaaaac gtagattagg tctaggaatc
      901 attaatggtg aataaactaa acatttgatt ttgataaata ttcgttactt taaacgctta
      961 ctatttatgt gagcgttttt tataatgcaa taaagaaacg gaggagagct tatgtataag
     1021 gataatgaaa tgaatattat ttatcgatca gaaaaccaac aatttcattt gtttaatcag
     1081 gagataagtt acatttttaa cagaattagg cagaagtcgc ccatcctcta taggtgggtg
     1141 atgaatgccg ttcggtatga gggttaccgt tggtaactcg aagaccgaca actaaggcgt
     1201 attgcctttt tattttgt
//
LOCUS       DAFGZE010000220         1218 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.229, whole genome shotgun sequence.
ACCESSION   DAFGZE010000220 DAFGZE010000000
VERSION     DAFGZE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.229"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            158..1198
                     /locus_tag="LTU54_003163"
     CDS             158..1198
                     /locus_tag="LTU54_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBL8297789.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          492 a          175 c          202 g          349 t
ORIGIN      
        1 ttaaaggtag tacataattc attttatttc tgattttagg tagtatttaa ttaaaaaaca
       61 gttttaatgc gtttgcattt tttatttatc tttgctattt tcgaaaagag taggtaattt
      121 tacataaata aaacaacgtg aaagtagagg atctacaatg actgatttta aattttttaa
      181 ggcggataga gtttacaacg aattatttta tcaatttcca aaagtcttta ttgtttctga
      241 cgaatacaaa aaaatgaaag attcaactaa gattgcctat atgcttttaa aagcaagatt
      301 agagatcgca atcagcaaac ggcaaatcga tgaagaaggt aatgtttatt ttacttatac
      361 gacaaatgaa ctatgtagag tattaaactg ccaaaaacaa aaagcgatag caatcaaaaa
      421 agagttggaa tcctttggtt tattattaca aaagcagatg ggatttaaca aacagttagg
      481 gaaaaataat cctaatagac tatatctagc agaattaaaa gtctcagaaa atgatatcta
      541 cttactcgaa aaatttgata gagagaatag ggaaaacgtt gataaatcag agggtatgaa
      601 aatcataccc accctcgacg aaaaatcaga cgctgaatcc cttggggctc aagagggtat
      661 gaaaatcata ccgtgccaaa acgttgataa atcagagggt atgaaaatca taccagaact
      721 taataataat atattagaca ctaatagaca caatatagac actgaaaaag accgcctaca
      781 agatcaattg ttgttagaca attttgagac aattatgaca aacgacagca ttgctacgtt
      841 tgtccctgaa cgagtattaa atttgataaa aacattttct tcaagttaca gtgaagctca
      901 aaaaaccgtc cagactattc ataatgcaaa gaaaaaagct gaaatagaaa gtgatatttc
      961 gatagttttt gaagaactcg atagttatta tgtcaatgca gaacaagaat tatacacgac
     1021 actgttaaaa gcctatcaaa aattaaaaac cgaaaaagtc gaaaatatcc agaacctgat
     1081 ttttgtctat gtaaaaaatt ggtttatcga aaaaccaata gctgctaaag tatcaagtga
     1141 aaaacgtttg aattatgcaa gctccccaag cactattacg aaagactggt tagagtgaaa
     1201 ctttactgac aaagtgat
//
LOCUS       DAFGZE010000221         1217 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.230, whole genome shotgun sequence.
ACCESSION   DAFGZE010000221 DAFGZE010000000
VERSION     DAFGZE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.230"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            4..963
                     /locus_tag="LTU54_003164"
     CDS             4..963
                     /locus_tag="LTU54_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HBL8297790.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRSDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          403 a          233 c          247 g          334 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagatcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctac
//
LOCUS       DAFGZE010000222         1215 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.231, whole genome shotgun sequence.
ACCESSION   DAFGZE010000222 DAFGZE010000000
VERSION     DAFGZE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.231"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(23..322)
                     /locus_tag="LTU54_003165"
     CDS             complement(23..322)
                     /locus_tag="LTU54_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297791.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(328..558)
                     /locus_tag="LTU54_003166"
     CDS             complement(328..558)
                     /locus_tag="LTU54_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297792.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            complement(808..1008)
                     /locus_tag="LTU54_003167"
     CDS             complement(808..1008)
                     /locus_tag="LTU54_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBL8297793.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          425 a          202 c          177 g          411 t
ORIGIN      
        1 ctaccttttt aggtagccta ctttaatctt tgtatctttc tggatcaacg aaagtatact
       61 ttatatagtc ataacgccga tgatcgcttc gagcgtctag cacatcagtc acgatatcaa
      121 acaaaaaata tacgtctttc ttcattctag ttttcgcagc agggatttta aagtagttct
      181 tattagaata gtagagatta attaataagc tatcttcgat tgctaaaaag aaaacttctg
      241 aatcccacac cttataaaaa tctttgacaa atctattcga aggatcaaat ttaaaccata
      301 attgcgtctc attaaaaagc ataaccatta ctccgatcta tttttaaact tagtctctac
      361 ctctaatata tatcgagttt ttattttgcc ttcagagaat actgtttctt tttttgcagt
      421 tacaggttgt ttattttcgg aaaaagctaa tatagctaaa attgaaacat ccatctggaa
      481 tttgtctttt tcgttgcttt gctcataaaa atctgcatat tcatcactaa tattttttct
      541 aataaattct tccatcataa agatcacctc gaaaagagta tacgaacaaa cgttcttttt
      601 gtaaaggtgg aatttattat actaaataaa taggtgaaca agtacttgtg ccaaattatg
      661 tgccaaaaaa aatcgaattt aataaaacac aaacaaaaag aaatcctatt atgataacat
      721 ttcttataat aacaaacaca acagaagacg tgtaatagtt agtcaggaac gtacaaataa
      781 cccctgtatc ctttgcggta cagggggtta ttttgtataa gtagacattg tagtttctaa
      841 actgctatgt cctaaacgtt tcgatacgct aagtatattt actccttgat aaagtaaaat
      901 agatgcatgc gtatgtctta gtccatcaat aaacagtcga agagaaaata gggaaaaatt
      961 gattactaaa gaaaaactta ctaggtcgac ggtgtagttg attttcatac ttcaatagat
     1021 tcgaattaaa cgatttcgat tggcttatac cagtcctatc caacgaaaga tatatcgttc
     1081 ttaactctag cggtataact tgaagcttta cgtaaagcca atgggaatga actggagatg
     1141 actttttatt atttttagta atagctgatg ggttaatact tacaactttc cttttcaaag
     1201 taccatataa aaaaa
//
LOCUS       DAFGZE010000223         1210 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.232, whole genome shotgun sequence.
ACCESSION   DAFGZE010000223 DAFGZE010000000
VERSION     DAFGZE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.232"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..208)
                     /locus_tag="LTU54_003168"
     CDS             complement(<1..208)
                     /locus_tag="LTU54_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297794.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIG"
     gene            complement(305..757)
                     /locus_tag="LTU54_003169"
     CDS             complement(305..757)
                     /locus_tag="LTU54_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297795.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(764..>1210)
                     /locus_tag="LTU54_003170"
     CDS             complement(764..>1210)
                     /locus_tag="LTU54_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL8297796.1"
                     /translation="NTVDLEIKNKLVDELTFYFDTVGFLDEHGVYHIKDFGAIRERIS
                     QDNEQGRAARVFYEHIIVPRMEKVDWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALK
                     AFNFEKAADLLLKIAEKQLGKAAAKELAKVNIVAGIAAAAASCALE"
BASE COUNT          431 a          235 c          123 g          421 t
ORIGIN      
        1 tgcctatctt ttttacaaat atttcatttg gtgtacagac taataaaaca atatttgtaa
       61 tcaatccttg cactaaagaa ttaaaaggaa gactatttaa aacagggtgt gcataactat
      121 aacatgtgaa gtagttattt tgaactttaa cttttaaaaa ctttaaaaat aatcttattt
      181 gttcttctaa acaatcatct atactcatat aatcactcca ctaattaatc tccccaaaaa
      241 gacacagcat taagagatat accaacacaa cagtgcaaat aactctacat cttccttcgc
      301 aaccttattt caataaacca taaaaattat tttttaatag aaactgaaaa ttaaaactag
      361 aaaaagtagt tttaccgaat gtaagagcat catcaccatc aatatagaaa tacattcttt
      421 tccagttaca tttaattttt aaacaaaatt ctccccaaaa aggaactttt tctagtgaaa
      481 acttggtaat ctctttgtaa ttaattcttg ttaaagaata attaaacact tcatttttac
      541 ttgttatttt ttcattgact ataactagtt catctggtaa aaaaattaac caacaaacat
      601 cgacaaacag taaatgatat aatccatttc cacctccaag catattcaat actggtgaac
      661 atgccttaga aacacgaata tagttatggt ttaaatagat attttcttta aacattgctt
      721 gtttaaaagc catatgctct tgttctaaac ttttcataaa cttttactct aatgcacagc
      781 tagcagcagc tgctgcaatt cctgctacaa tatttacctt agctaactct ttagctgcag
      841 cttttcctaa ttgtttttct gcaattttta ataataaatc cgcagctttt tcaaaattaa
      901 aagcttttaa tgcttcaata aaatctccct gcgtacctat agcccaagcc actcctcctc
      961 caggaagaga attaatggct acacatccag catatcgttt ccaatcaact ttctccattc
     1021 taggaacaat aatatgttca taaaagactc ttgctgctct tccttgttcg ttatcttgac
     1081 tgattcgttc acgaatagca ccaaaatcct ttatatggta tacaccatgt tcgtctaaaa
     1141 aaccaactgt atcaaaataa aaagttaatt catctactaa tttattcttt atttctaaat
     1201 caactgtatt
//
LOCUS       DAFGZE010000224         1193 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.233, whole genome shotgun sequence.
ACCESSION   DAFGZE010000224 DAFGZE010000000
VERSION     DAFGZE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.233"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..1076
                     /locus_tag="LTU54_003171"
     CDS             <1..1076
                     /locus_tag="LTU54_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL8297797.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DAFGZE010000225         1188 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.234, whole genome shotgun sequence.
ACCESSION   DAFGZE010000225 DAFGZE010000000
VERSION     DAFGZE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.234"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            474..725
                     /locus_tag="LTU54_003172"
     CDS             474..725
                     /locus_tag="LTU54_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297798.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="LTU54_003173"
     CDS             759..1037
                     /locus_tag="LTU54_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297799.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
BASE COUNT          428 a          162 c          220 g          378 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatt
//
LOCUS       DAFGZE010000226         1177 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.235, whole genome shotgun sequence.
ACCESSION   DAFGZE010000226 DAFGZE010000000
VERSION     DAFGZE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.235"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..851
                     /locus_tag="LTU54_003174"
     CDS             <1..851
                     /locus_tag="LTU54_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBL8297800.1"
                     /translation="HMHMSYRGSFSRKYVLNKWFRLLNIPVIIHLHGSEFKKWYDSVS
                     VKKQNQIRLLLRESDSTIVLGEKWKKIIKEIEPNCKTVVVENSIVVPEYTIKWNSKMC
                     RFLFMGVLIKRKGVHDLVEAVKNIAEQYPEKKFIFEIAGAGDEEISLKELIEEYNLQS
                     YFEFTGWVVGEKKEECYRRSNVMILPSYNEGLPISILEAISYGMPVIATDVGDISSAV
                     RDGENGYLFQPGDVSALTSAILKINDKDKYVKFSRCSKDLAITKFNDGNFFDRILNLY
                     QKGSKE"
BASE COUNT          435 a          122 c          251 g          369 t
ORIGIN      
        1 ttcatatgca catgtcgtac agagggagtt ttagtaggaa atatgtgctg aataagtggt
       61 ttagattatt aaatattcct gtaatcatcc atcttcatgg atcggaattt aaaaaatggt
      121 atgatagtgt ttctgtaaaa aaacaaaatc agattcgctt gctgttaaga gagtctgatt
      181 ccacgattgt acttggagaa aaatggaaga agataattaa agaaattgag ccaaattgta
      241 aaactgttgt tgtagaaaat tctattgtag ttccagaata taccataaag tggaattcaa
      301 aaatgtgccg atttctgttt atgggagttt tgataaagcg aaaaggtgta cacgatttgg
      361 tagaagcagt taaaaacatt gcagaacaat atccagaaaa aaagtttatt tttgagattg
      421 ctggagcagg tgatgaagaa atatcgctca aagaattgat agaagaatat aatttgcaat
      481 cgtattttga atttacaggt tgggttgtgg gagaaaaaaa ggaggagtgc tatagaagat
      541 caaatgtgat gattttaccg tcctataacg agggattgcc tatatctatt ctagaggcga
      601 tttcttatgg aatgcctgta attgctacag atgtaggaga tatttcttca gcagtacgtg
      661 atggagaaaa tggatatttg tttcaacctg gtgacgtatc agcgttgaca tctgcaatac
      721 taaaaatcaa tgataaagat aaatacgtaa aattttcacg ttgttcgaag gatttggcaa
      781 taacgaaatt taacgatgga aatttctttg atagaatttt aaatctgtat caaaaaggga
      841 gcaaggagtg atgacaagga aaataaaaaa tgtttcgatt atgtaatgaa cttattaagt
      901 gaataaaata gagagaaaaa atattgaaat agacgatgta aatttttatc cgtgtaagaa
      961 ttcaaatgaa tagtagtgtt ttagtgcttg taatagagca agagtacaag cacggtacat
     1021 tatataaaaa cattaatgat atttctataa ataaatagat tttgtattct ggatgatgaa
     1081 gaggaagata ttatctgata acttacccta aactttgcag ggggtggtta taagttgtgt
     1141 atatgtggca gtaatgaatg agtgaaataa tcatatt
//
LOCUS       DAFGZE010000227         1147 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.236, whole genome shotgun sequence.
ACCESSION   DAFGZE010000227 DAFGZE010000000
VERSION     DAFGZE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.236"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            237..827
                     /locus_tag="LTU54_003175"
     CDS             237..827
                     /locus_tag="LTU54_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HBL8297801.1"
                     /translation="MEISSKEYLKAVLRMEKNLYIESKGQHTKLLKIMKSHPDYNAWR
                     YTRRMRITSYYYSKRKKNIIYAIMYIVSCRRMNSLGRKCGIECGENVFDEGVRFYHTQ
                     GIVINGNAIIGKNCYLYGNNCIGNDGIDPKCPVIGNNVRVCVGAKIIGNVKIANNVVV
                     AAGAIVIKDCLENGAILAGVPAKIIGYVNLTKVFLE"
BASE COUNT          415 a          127 c          234 g          371 t
ORIGIN      
        1 taaaaattcg ttaggtttaa ataactggaa atatcggaaa atgggcatta tacatcacgg
       61 acatcccttc ttttggaaaa aatgttacga tttttataag taatattgta tccggtggaa
      121 tcaacgatga agataacata tccatcgttg tagattcagt agcattgaat gatgctcttg
      181 ttgcaagaaa tccgaataaa attgttaagt ataaattgaa ttaataggag atatgaatgg
      241 aaattagcag taaagaatat ttaaaagcgg ttcttcgtat ggaaaagaat ctatatatag
      301 aatctaaagg acaacacaca aaattactaa aaataatgaa atcacatccg gactacaacg
      361 cttggcggta tactcgtcga atgagaatta catcttatta ttattcaaag agaaagaaaa
      421 atatcattta tgcgattatg tatattgtgt cttgcagaag aatgaatagt ttgggaagaa
      481 aatgtggtat agagtgtggt gaaaatgtat ttgatgaagg tgtaagattt taccatacac
      541 aaggaattgt tattaatggt aacgccataa taggaaaaaa ttgttactta tatgggaata
      601 actgtattgg aaatgatgga atcgatccta agtgtcctgt aattggaaat aatgtacgtg
      661 tatgcgtggg tgcaaagatt attggtaatg taaaaatagc taacaatgta gttgttgcgg
      721 caggagctat tgttatcaaa gattgtctag agaatggtgc aattcttgca ggtgtaccgg
      781 caaaaataat agggtatgtt aatttaacaa aagtattttt agaataattt ttagcgaaag
      841 aaggtgaata acgatgcgta tttcaataaa aagatggtca ggagcagatt ggacattgtt
      901 cacgttggca ttttttgcaa tctccatagt tcttgtatat acttttaacg ccccaagagc
      961 aattttatat ttgggagata taattaatat ctttgtgtta ttaaatgcac ttagctttag
     1021 acgcagaaaa ggaatatatg ctagcaaaaa tgagatgctg atagttttgt ttttactagt
     1081 ttctatttgt agctcagtta ttaatttaga aaatatagga ttgattatct gggggatacg
     1141 aaataac
//
LOCUS       DAFGZE010000228         1137 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.237, whole genome shotgun sequence.
ACCESSION   DAFGZE010000228 DAFGZE010000000
VERSION     DAFGZE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.237"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..355
                     /locus_tag="LTU54_003176"
     CDS             <1..355
                     /locus_tag="LTU54_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297802.1"
                     /translation="GAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETNMTRDELL
                     RKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTTSQIKQDT
                     EDSLRKGWGMNNKVR"
     gene            615..910
                     /locus_tag="LTU54_003177"
                     /pseudo
     CDS             615..910
                     /locus_tag="LTU54_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            985..>1137
                     /locus_tag="LTU54_003178"
     CDS             985..>1137
                     /locus_tag="LTU54_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL8297803.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          431 a          135 c          237 g          334 t
ORIGIN      
        1 tggcgcacca atcagaaagt ttgataaggt ttattccacc aagcatcctt taaaactgtc
       61 gactttgtta aatgcgactt ccagagtttt agtagaaaag tatgagacta atatgacaag
      121 agatgaattg cttagaaaag ctttagatga gtatattaaa cagaatctta cgcaagaaga
      181 taagcaagat ttgtttaatg acgtggtgaa agaactagac ttgttcagag aaaaatatcc
      241 aattgttcca gaattagatg atcgtggaga aataataaga actaccagtc agataaaaca
      301 agataccgaa gactcactgc gcaaagggtg gggaatgaat aataaagtta gataataatc
      361 aaagaagaac taagtaacta cctttaaacg gtagttactt agttcttctt tgatttgcat
      421 gaaatagagg ggatttattt ttctggttac gtgattaaaa aatccaaaaa atgagcatag
      481 gaacggttat gattttaggg tgtgttttaa tgagatattg tgatttcatt aacaagtgta
      541 tgataatctg ttgttgagtt agtaaataag taatagagct aactctaaaa taaaactatt
      601 aggaggagat gtaggtgaaa aagtttaaaa agaaaatata tttaggattg gttgttgggg
      661 tgagtatgtt tgggttcatg gctcatacta aacaagtaac tgcagatact aaccaaaaag
      721 aaaggattat gagccaatta gatgactctc aaataagtat gtacccaatg agctaattgt
      781 cagtttaaag gctacacaaa gaatttcaac agagaaaaat aaaccgcaaa atttattggg
      841 tgtaaaagtt atggtggaaa aagatttgac tcttaggaca aatgaaagcg attgtaataa
      901 gcgagatcaa gggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag
      961 aaaaaagaca gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac
     1021 gtcattattg tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag
     1081 gaattgttat atgatcgtgg aataaatgtt tgtcatacta cgatttatcg ttgggta
//
LOCUS       DAFGZE010000229         1109 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.238, whole genome shotgun sequence.
ACCESSION   DAFGZE010000229 DAFGZE010000000
VERSION     DAFGZE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.238"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..351
                     /locus_tag="LTU54_003179"
     CDS             <1..351
                     /locus_tag="LTU54_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297804.1"
                     /translation="QDLIFYIHEDKVKDHVKDEDYEQHAINYKVKGYKKEQKAAQKQI
                     ESFQATIDLNEELVKKLKDQLPYQVAEDQENTENKINSYQQEIETSKTQMKDQEESIQ
                     KLQEKIDRMTKENI"
     gene            374..603
                     /locus_tag="LTU54_003180"
                     /pseudo
     CDS             374..603
                     /locus_tag="LTU54_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            624..>1109
                     /locus_tag="LTU54_003181"
     CDS             624..>1109
                     /locus_tag="LTU54_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297805.1"
                     /translation="MKKTYQKFLWISYIVLFIVGFLVSVSVIVGTLFLLFSDEDLVLK
                     GLMSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIEYTWLPLKRNSKR
                     KEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQIAASNIFTAVMTL
                     MC"
BASE COUNT          428 a          153 c          220 g          308 t
ORIGIN      
        1 caagatctga ttttttatat tcacgaagat aaggtcaaag atcacgtgaa agacgaagat
       61 tatgaacaac atgcgatcaa ttacaaagtg aaaggataca agaaagaaca aaaagcagca
      121 caaaaacaaa ttgaatcctt ccaagcaacc attgatttga atgaagaatt agtgaagaaa
      181 ctaaaagatc aattgcctta tcaagtcgca gaagatcagg aaaatactga aaataagatt
      241 aatagttacc agcaagaaat tgaaacatcg aaaacgcaaa tgaaagatca agaagagagc
      301 attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag aaaatattta gaagaatagg
      361 tggagtaaaa gaaatgaagc gagcgaactt ggtcggcaaa gatactttcg ctgagcagaa
      421 agtattgatt aaagcctatc tcaaagttgt aaaagctaaa gaaatatgtc aagaagtgaa
      481 ttatctaaca caacaatatc tgagttgaag aatggtagaa agagtttaga taccttaaaa
      541 ttcagatcat ttgaagctct atatatagct gctaaaaaat attttacagc tgataataaa
      601 taaaaaatta aggtgggttc tatgtgaaaa aaacgtatca aaagttttta tggattagct
      661 atatcgtcct atttatcgtt gggtttctcg tcagtgtgtc cgtgatcgtc ggaacgcttt
      721 ttcttttgtt ctctgacgaa gatctggtac ttaaggggtt aatgagtacg ttatcgtgtg
      781 tcatgttact ttatttatgt atacagatca acgaaattat gccccctcat tggcaagaaa
      841 agaagaaaga atttaaggaa gaaatcgaaa taaaatccaa tgactggatt gaatacacat
      901 ggcttcctct gaaaagaaat agcaaacgaa aggaattttg ggcattgcaa ttatggccaa
      961 taggaaaact ttttatttat atggcttcac ttatggttac tttaatcggt tatttggtgg
     1021 tggtctatgg ggtggcctat tacttgttaa gtgagccaaa acagatcgcg gcgtcaaata
     1081 ttttcacggc agtaatgaca ttaatgtgt
//
LOCUS       DAFGZE010000230         1098 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.239, whole genome shotgun sequence.
ACCESSION   DAFGZE010000230 DAFGZE010000000
VERSION     DAFGZE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.239"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(72..1028)
                     /locus_tag="LTU54_003182"
     CDS             complement(72..1028)
                     /locus_tag="LTU54_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297806.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          357 a          182 c          164 g          395 t
ORIGIN      
        1 aagttttgtc tcgcttaatt tgacaaataa tatttttaga aaaagcagtc ggactttcta
       61 aaagaagttc actattcgat cctgtcgatg atttcttcag gaaaatcgtc tatttgcttg
      121 tcaattttgc tgtctttgta tttctcttct tggatagtca acaaaatctt tgtaagattc
      181 ccccagtcta ctgctcttgg cggatcgagc caaaccacta cttgagatgc actttcttca
      241 tatagattat tcctattcgt gattataaca tccgggaact caggctttcc tgcctctatc
      301 acttccgtgt tcagattaat gtacaagctt aaatttttct tgataaattg attataggca
      361 gcaccataag aaaaatcgat gatgatcttt actggttcag aaatcagttg aatgggtatg
      421 tgattgatca ataataataa ataactgaaa aaaagaaaaa atttatcttg aagcaactct
      481 ttgcgttttg acgttagatc atttatatac tctatcaaat agcaataaaa tgctggatag
      541 ttttctctaa aaaaagaaac atcaagtgga aattcatcag ctttattaat agtgtaatgc
      601 aaaagtttaa aatgtatttt ggttaactct cttcggatca attggctcgt ctcttctcgc
      661 aacagattaa actgattttc aaaactttca atgaatcgtt cattcaacca ttcgattgtt
      721 tcatcttcat tttttctcaa gtatttttga tccaatttat catgaagata aagccaagag
      781 agtgtcttat tgataaaaat aacggatttt tctcctaata ctccttttaa gtggtaaaat
      841 cctatatccg aaaaaaatag cgactgattg ctagcaatat ctaaagaatc agcttgttca
      901 gccaacaggt tgattgctaa aaagtggaat aatttctttt tttcataagc agacagagga
      961 taatactgtt ttaaatcttt taacattttt tctgttttta taattatagg tagcgtatac
     1021 aattccacaa aacttatctc tgttgagtta aaaagattat ataaaaaaag ccgaatgttt
     1081 ttaggattcc ctgtcaac
//
LOCUS       DAFGZE010000231         1087 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.240, whole genome shotgun sequence.
ACCESSION   DAFGZE010000231 DAFGZE010000000
VERSION     DAFGZE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.240"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            64..849
                     /locus_tag="LTU54_003183"
     CDS             64..849
                     /locus_tag="LTU54_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HBL8297807.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          427 a          161 c          177 g          322 t
ORIGIN      
        1 cttttagaaa aagcaaaaaa aaaagaaaaa atcttattct tgataacaag ctacgtgacg
       61 tatatgccta acaaaaatag aacttttgaa tcaatcttaa atgaaggttt aaccacagaa
      121 ccggcatcca ttatttttga tgaagttatc gatgacgttg aagatagtaa gtatcataaa
      181 aatttaaaag aactttttta ttttgcactc aacaatggac caagtttgtt cactggtata
      241 tcagtacctg atagtgataa tccattaatt atggcaaaaa tttacttaac aaaatggtgt
      301 aataaataca tcagggatag gaataaccca gcattaaaaa aacctcttaa aacatttggc
      361 gaaaaagatg ctgctttaac tactagagta gcagcaaatg ctaatattga tgatcaaaaa
      421 gtattgaatg actacttgcg tggacatttt ttatatatga gcgctgagaa catgaacgga
      481 agtattttag aggaatattt agccgaagtg ttagaacctg aaggttggat ttggtgcgcc
      541 ggctctgtat atcgagctgt tgatttctgt tatttaggaa cctcaccaat tcttttacaa
      601 gtgaaaaata aatacaatac agaaagcagt tcgagttcag ctataagagt aggaacaaca
      661 attagaaaat ggaatcgttt aaataagtcg acaaaaattt ctggtttgga tagccctatc
      721 cccaattgga aagcattaat agaaatgact gaagctagta aggaactggc agctaagtta
      781 actgaaaata gttatttagc ttatattaat gaaaaatcca cacgagaatt atggacactc
      841 gatgactaac aatatcctta ttagtatcta tccacgatac atggaaaaaa ttataaacaa
      901 acaaaaaaac tttgaattta gaaatttcta tcttacctca gatccttata atatcttttg
      961 gatttacgaa acaatcccca ccaaagcttt aaagtataaa ttgattgtaa aaaatccaat
     1021 tactaaactt aagaaaaatc aacactactt tttaggagac gatgagtttg aacaattagt
     1081 ttaaaag
//
LOCUS       DAFGZE010000232         1087 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.241, whole genome shotgun sequence.
ACCESSION   DAFGZE010000232 DAFGZE010000000
VERSION     DAFGZE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.241"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            1..317
                     /locus_tag="LTU54_003184"
                     /pseudo
     CDS             1..317
                     /locus_tag="LTU54_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            472..1047
                     /locus_tag="LTU54_003185"
     CDS             472..1047
                     /locus_tag="LTU54_003185"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBL8297808.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
BASE COUNT          384 a          156 c          231 g          316 t
ORIGIN      
        1 ggggaacttc atctccagat tccgcgcgac cgcaatggcg agttcaagca acagactgtt
       61 cctgcttata gacggacgaa tgacacatta gaggagaccg tcattcacct cttccgaaaa
      121 ggtattacca tgtcggaaat cgcagacttg attgagaaaa tgtatgggca tcactacacg
      181 ccccaaacca tgtccaacat gactaaagtg ctgactgaag aagtaaatgc atttaaagtt
      241 agaactctaa atgataagta tgtcgctatt ttcatgattc cttcagtttt tcagatgata
      301 taccactcaa aaaacaatat gagagatgtt caggaatacc atctctaatg gcttataaat
      361 tggttttagg gatagtagat gttatagaca tatcaaggaa taaacgagca gctgcataaa
      421 agcagatgga attaaggaat aattgaattg taaaagaagg gaataaattt aatggcacaa
      481 ttattttaca gatatggaac aatgaatagt ggaaagagta ttgagctact gaaagtggct
      541 cataattatg aagaacaagg gaagagagtt atcataatga caagcgcaat agatactcga
      601 tcaggatttg gaaaagttga gagtcgtata ggtgtttcga aaccagctat tgcaattact
      661 tctgaaatgg atgtgtacgg agaattagtg gagattattg atcagtacgg aaaagttgat
      721 tgtattttgg tcgatgagtc tcaattttta agtagagaga atattattga tctatcactt
      781 atcgtaatga atgaggaagt accagtaatt tgctatggat tgaagaatga ttttcaaaat
      841 aatctcttca agggatccga agcattattg acatttgctg ataaaattga ggaaattaaa
      901 actatttgcc atttttgcaa taaaaaggct gtaatgaatg ctcgtattgt tgatggtatt
      961 ccagtttatt caggtgaaca gattgtagta ggaggtaatg aagattacct ttcggtatgt
     1021 cgcaaccact actacaacga agattgaaat aaaaaacgtg taatcaaata attgattaca
     1081 cgttttt
//
LOCUS       DAFGZE010000233         1084 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.242, whole genome shotgun sequence.
ACCESSION   DAFGZE010000233 DAFGZE010000000
VERSION     DAFGZE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.242"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            69..686
                     /locus_tag="LTU54_003186"
     CDS             69..686
                     /locus_tag="LTU54_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297809.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            690..>1084
                     /locus_tag="LTU54_003187"
     CDS             690..>1084
                     /locus_tag="LTU54_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297810.1"
                     /translation="MTDIVKIKQSGVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATD"
BASE COUNT          395 a          163 c          236 g          290 t
ORIGIN      
        1 ttgattattg caactgttga tgatccaaaa cagaatgttt tcactcttga gaaagttggg
       61 gtgatttgat ggttaaagta aagagaatga tggagactga tgaatctggt gttaaacgtc
      121 aatttatgcc aattacacat gtttctgcag ttttaggctt agaaaaaatg atatcaggac
      181 aatcaaaagt tctttcggtt aatggtaaat atggtgcagt cattttaacg aaagctgatt
      241 taggattaga gaatgcaatt actgaactac cttatgcaag cgagacaagc gatggtattc
      301 ttactgctga aatgtttcaa aagatcgtaa atggagaagg tggaacatat attttgccga
      361 ttgctactcc tgaacaatta ggaggtataa aagttgggga actcttagaa gttactgaag
      421 aaggtgtttt atctgcaact aagcaaacag acttcaattt ctctgaagaa ttaaagggaa
      481 aattagaatc tctaaaaata ttaaaagcag gtgcaaatat atcaattgca gaagatggaa
      541 caattagttc tacaggtggt agtgggactg gtggtgttaa tcagtcatat gtagatcaaa
      601 aattccaaga agctgtaaat caagcagaaa attacacaaa cgaaagaatt ccaaatttta
      661 cttttgaaaa aatcggggag gtatagaaaa tgacagatat cgtaaaaata aaacaaagcg
      721 gtgtacaggt ttatccacaa actcattgga atgctataga aggcaaacca acaacggtga
      781 agggagataa aggcgatcct ggccaagcag caacaattac cataggaacc gtttctagtg
      841 gttcaaccgc ttctgttacg aatgtaggaa catcatctgc agctagattt aattttgttt
      901 taccaaaggg agataaaggt gatcctggaa taaatgccac aactacagct gtagctacaa
      961 ctacagcaaa tgggttgatg tcgtccacag ataaaactaa gttggatgga attgcagctg
     1021 gagcacagaa aaatccaggt aatgctacaa ctacgacagc aggtttaatg tcagcaaccg
     1081 ataa
//
LOCUS       DAFGZE010000234         1073 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.243, whole genome shotgun sequence.
ACCESSION   DAFGZE010000234 DAFGZE010000000
VERSION     DAFGZE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.243"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>1073
                     /locus_tag="LTU54_003188"
     rRNA            <1..>1073
                     /locus_tag="LTU54_003188"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DAFGZE010000235         1065 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.244, whole genome shotgun sequence.
ACCESSION   DAFGZE010000235 DAFGZE010000000
VERSION     DAFGZE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.244"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            62..676
                     /locus_tag="LTU54_003189"
     CDS             62..676
                     /locus_tag="LTU54_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBL8297811.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(841..>1065)
                     /locus_tag="LTU54_003190"
     CDS             complement(841..>1065)
                     /locus_tag="LTU54_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297812.1"
                     /translation="DFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVS
                     TYKELYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          351 a          191 c          186 g          337 t
ORIGIN      
        1 atggcttcct cgtgtatgat tagggtgtat tttaaatagt tgcagaaagt aggtaaaagt
       61 tttgagagtt gcatacatac gtgtctcatc gatcgatcaa aatgaacaac gccagattga
      121 agaaatgaaa aaattcggtg cagagagaat tttcatcgag aaacaatctg gcgcaacgat
      181 tactcatcga cctgttttcc aagaagcatt agattacgtc cgagatcaag atatttttat
      241 tgtggaagct attgatcgac ttggaagaaa ttatgacgag atcatcgctt cagtcaatta
      301 cttgaagaag aaaaatgtga agctgatcat tactagttta ccgatcatgg ctgaagcaat
      361 tggcaatcct ctcctggata aattcattaa ggatctgatc atccaaattt tggccatgat
      421 cgctgaacaa gaacggactg aatcgaaacg tcgccaggca caaggaatta agatcgcaaa
      481 agcaaacggc gtctacaaag ggcggcccaa attatacagt gcagacgcaa aagatcccca
      541 acgtcgcctt gtttacaaaa gcatcgttga agatcttaaa aatggtgttg cgattgctaa
      601 gattgctaaa gactacaatg ttacgagaca aaccgtgtat agaataaaaa aagacagcat
      661 ggtcaatgac aaatagttga tcatgctgct tttattatct tctcatcttt aatttcctac
      721 atataattac tatctataaa attaagtctt actcagaaca gataaagcat aaaattccaa
      781 caaaccattt tttacttaac taaataaaga gtctgttaat ataatcttca cttatttacg
      841 ttaatcccct actgtatttg ttggttcctt atctatattt tctacaaaat ccttattatc
      901 ttgtgattta gcttcgtata attctttgta tgtcgacaca ttggttatac taattcctat
      961 agctagttct tgagactcta cttttctctc tagctccttt gacaaagtga tttgagattt
     1021 atttggttcc gttggagtca attctataga ttcttcataa aaatc
//
LOCUS       DAFGZE010000236         1062 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.245, whole genome shotgun sequence.
ACCESSION   DAFGZE010000236 DAFGZE010000000
VERSION     DAFGZE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.245"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            11..418
                     /locus_tag="LTU54_003191"
     CDS             11..418
                     /locus_tag="LTU54_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297813.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            432..833
                     /locus_tag="LTU54_003192"
     CDS             432..833
                     /locus_tag="LTU54_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297814.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            835..>1062
                     /locus_tag="LTU54_003193"
     CDS             835..>1062
                     /locus_tag="LTU54_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297815.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKEL"
BASE COUNT          395 a          135 c          192 g          340 t
ORIGIN      
        1 ggagaaatca atgtcttata gagtacaatt aataatttca gaagatgtag aaagtcagca
       61 atttggcact aatgttatta ataaagtaat taatccagct ctttctatta atgcgccttt
      121 aattcctact gctttatcat tttctgtaac agctgtggtg tctgagatag aggatacaga
      181 aaagataaaa atagtagaaa ttgaagtttt aaacaagaat gaaaaacaaa ttttttctac
      241 gggtgaagta tcggtaagct tgcctcctca agttaacgat ataaacttta atataaacgc
      301 cagaaatgtc ttggttgaag aagcaggaga gcattatgct gttgttaaat ttaatggaac
      361 tgagattgga aggcaaatct ttgatatcaa ggtcaacaaa ccagtggaaa aaaattaagg
      421 agacgatacg gatgcttgat atagtaccat caaatacaca tcggagtggt agcttaataa
      481 attttgctgc gatttttgct tgcgcatcgg taattctagc atctccagtt gttgtggatg
      541 caagttctac tccgaaaact aaaaatgata atcaacttgt aattactaca aaaaatgaga
      601 ttaacacaat ttcaagtaat gatggcaaaa tttttgatgt gataaatacg gttttaaaaa
      661 gaaaagctga aatcaatata aaatatgatg aagatttaaa cttgtatttc tttgttatca
      721 aaacaacatc tgaattgttt tcctcagatt acgatgtttt ggatactctc gataatgtat
      781 tatctgacta taaatatatg gggaaatctg tagtagcaac tttgggggaa taaaatgttt
      841 gactttaatg aatatttatc tatttccaaa caaattgaat cagatgataa atataattct
      901 aaagaatctt atagaagaac tgctgtttca agagcatact acagtgcttt taaaaaatct
      961 gatgagtatt tgaaagaaaa ttatgatatt atttataatg gaagtagcgg aaaaggaagc
     1021 caccaaaccg tttggaattt gttttcaaca gttaaggaat tg
//
LOCUS       DAFGZE010000237         1046 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.246, whole genome shotgun sequence.
ACCESSION   DAFGZE010000237 DAFGZE010000000
VERSION     DAFGZE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.246"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            217..>1046
                     /locus_tag="LTU54_003194"
     CDS             217..>1046
                     /locus_tag="LTU54_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBL8297816.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          372 a          150 c          178 g          346 t
ORIGIN      
        1 tatagcaaag aaagtttaat aagagtttgg acctagcaaa caatattaat gaggtcatta
       61 aatcttatta tctttagtga tataacgaaa tatctacaca gacaaaatga tagcgtaaaa
      121 tattttgtgt aaatagatat ttagggtaga aaaaaagaaa gtccattctg taaaattaaa
      181 gttaccacaa ccgaaatttt ataggaggac tttctcatgc ccaattttac tacagaaatt
      241 atggaaacac taatcaataa aggtgattta gatgaattat ttcgatgtca tttagaatta
      301 gctgttaatt cattattgca ggctgaatta acagcttttc ttgattatga aaaatatgat
      361 cgtgctgggt tcaattcagg taattcccgt aatgggaatt attcacgttc attcagaaca
      421 gagtatggag aattaaattt ggtaattcct agagatagaa atggtaagtt ttctcaacaa
      481 actttacctg cttataaaag aaccaatgac tctttagaaa ccactattat tcagctattt
      541 caaaaaggaa ttactatgtc tgaaatctct gaattgattg aaaaaatgta cgggcatcac
      601 tatacgccac aaaccatttc aaatatgact aaaatagttt ctgaagatat tattgccttt
      661 aaagaaaggt ccttagaatc cagatactct gtcattttta tggatgctac tcacattcct
      721 ttaaaaagac aaactgtttc aaaagaagca gtttatattg taattggcat tcgattagat
      781 ggaaccaaag aggttcttgg gtttagtctt gctccaactg aatctgctta tgtttggaaa
      841 gagattcttc aagacctaaa agaccgtggt ttaaaagagg ttttattagt cgtaactgat
      901 ggtttaagtg gcattaacga tagtattcat agtgtttatc caaatgctca atttcaacaa
      961 tgttgtgtcc atatatctag aaatatcgct cataaggttc gtgttagtga tcgacaagaa
     1021 atctgtagcg atttcaaatt agttta
//
LOCUS       DAFGZE010000238         1021 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.247, whole genome shotgun sequence.
ACCESSION   DAFGZE010000238 DAFGZE010000000
VERSION     DAFGZE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.247"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..211
                     /locus_tag="LTU54_003195"
     CDS             <1..211
                     /locus_tag="LTU54_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297817.1"
                     /translation="RRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTY
                     NQKFLNRSHKGFQQVTDTLVSMFTE"
     gene            complement(416..706)
                     /locus_tag="LTU54_003196"
     CDS             complement(416..706)
                     /locus_tag="LTU54_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HBL8297818.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
BASE COUNT          362 a          171 c          148 g          340 t
ORIGIN      
        1 tcgtagaacc atttactcaa ccaatctaat cgagtctttc aataagcaaa ttaaaagata
       61 cagccgtaga aaagagcagt ttcaaaatga agaatcacta gaacgctttc tagtcagcat
      121 ttttgataca tacaatcaaa aatttctaaa cagaagccat aaaggttttc aacaggtaac
      181 cgatacatta gtttcaatgt ttactgagta actaattatt ttgcaggagg acaatttatt
      241 tacacaaaat tattgacgct ccctttgcga gcagcaacta gaataattta aacattatta
      301 aactgtctat attgttacta ataattccct tcttatagtg cattttctta gaaaaaaaca
      361 atattacgat atcatgatat taagatatcg taatattata caattgtgat tgatattata
      421 tatgtttttt tattttttta tcatgtttaa tcttgaagga atcatattct ttttctaaag
      481 acaggagctt ttcataaaca tcagacatgt ttaaactaga ggccaggctt aaatcataaa
      541 cgaatgaagc aggtaatgat tttattctac gttgtcgggt tatccagctt gcaaccgttc
      601 cttgtgggta ggcatataac tcacaaaatt gttctacagt gattcctaaa ttcttgataa
      661 tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat agacatggtg cgtaagccta
      721 ctttctttta ttattgcgtt tgcaataata gtttaacaca cgcattattc tagtatctac
      781 tcctatttgt aaaaacaaaa aaggattcat agagataact ctatagaatc cgtaaaatac
      841 cttatatata taacgggaaa atataaaacc taaaattatg ataacctgct gcagcttcta
      901 catagaaaga gaagcgctca acaccaacct catttatcaa acgttggcca taaccccaaa
      961 tctcttctga tagctgatca aaggttatat actttagctc atattgctgg ataatgttca
     1021 t
//
LOCUS       DAFGZE010000239         1010 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.248, whole genome shotgun sequence.
ACCESSION   DAFGZE010000239 DAFGZE010000000
VERSION     DAFGZE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.248"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..132
                     /locus_tag="LTU54_003197"
     CDS             <1..132
                     /locus_tag="LTU54_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8297819.1"
                     /translation="NWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
     gene            complement(116..709)
                     /locus_tag="LTU54_003198"
     CDS             complement(116..709)
                     /locus_tag="LTU54_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBL8297820.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVSGGMATVFLTNF
                     IRCRTSFRMLFRLSLTMSLLGCDDLRQELTVKVRLPR"
BASE COUNT          304 a          211 c          171 g          324 t
ORIGIN      
        1 aattggtgga accaccttcg gttgcacggt acacttggct acgagacacc ggttggttac
       61 cgtaaccaga gattggcgca gcgaatcctt gataatgagc tcggatgtgc taacgctagc
      121 gaggcagtct aactttaact gttagctcct gccgaagatc gtcacatccg aggaggctca
      181 ttgtcaagga caatcggaat agcatacgga aagaagttct gcaccttata aaatttgtca
      241 aaaaaactgt tgccattcca ccagacactt gcttaaagcg aaaaatttct tcaatgtcca
      301 tgcctaattc tttggatatt ctcttgtctg accatccatt ttttactaat ctttggatga
      361 caatttccat tcctcttatt tcatgggtcc ctcttgcacg attgtgtcga attgttgaag
      421 ccatacgttc ttctatgggt ttatcaataa ctgttaccgg tacaactttt aagttgagtc
      481 tttccttagc aacacgatac ctatgaagca catcaaccaa gatatacttc tcttcttttt
      541 ccttgtaata acaaactaat ggttgagtaa agccatcttc aatgatagaa agctccaata
      601 agttcatttc cggagacgct actcgattgg gattataatc gttcgcctgt attttggtaa
      661 ttggaacaag ccgaacgtcc atcacaggga aagttatgtc tttattcata cgcttgtata
      721 ccctactttc ttaaaaagta gggtatactc aagaatccga ttaattatct tctcaatttg
      781 gataatatat ctcatcagtc ttcttgtcac tttatcaaca tgattttgct gcaaaatggg
      841 ataccgacag tactggactg gtaatcagga taaaagcaaa aataattaat ttgtatccta
      901 aaaaatccat aaaaattctc tcacattttc gggtattaat tcgagaatca cacctataaa
      961 tttattacta tataagggat acttaaacca aataccccat ctaattattt
//
LOCUS       DAFGZE010000240         1002 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.249, whole genome shotgun sequence.
ACCESSION   DAFGZE010000240 DAFGZE010000000
VERSION     DAFGZE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.249"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            369..1002
                     /locus_tag="LTU54_003199"
                     /pseudo
     CDS             369..1002
                     /locus_tag="LTU54_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          182 c          183 g          295 t
ORIGIN      
        1 tttcaaagtt agtagagaaa atgtagttat atatttaaat ttttaccaga aagaaatgaa
       61 caccttcaat tatctaaatc tcgaaaatgg ttagatgtaa tcatcaggaa aaacaatctt
      121 aaacgaatta ctattcatgg tcttagacac acacatgcta gcttgctttt agcaagctag
      181 tgcaaatatt aaagatgtgc aagagcgttt aggccactct tctattcaaa tcattatgga
      241 tctatatatt catattacag acaaaagaaa agaagaaacc gtagcgcaat tcgcaaaata
      301 taggctctat aatttatggg actgatgaat caaaattgat tcttcagtcc cattgttatt
      361 ttaggtatat gattcgtaca ttgaaagagt tgaaaaacta tcttccgaag ccgtcaaaaa
      421 ggaccctgcc aaaagtattg atggtggatg aatttcgttc gcacgcttca attgtggata
      481 aaatgagctt tatttgtgcg gatggcgaga ctggaaaact cattgatatt ttgcctacac
      541 gaaaattgtc tcgattaaca cactatttta gtcgctgtgc aaatcctgaa gcggtggaat
      601 atttagtgac agatatgaac gccgcctact tccaactgac aaaacgtgtc ttaccaaatg
      661 cgaaagtcgt cattgattgt tttcatatca tcaaacattt gaatcaggcg ttcaatgatt
      721 ttcgtgtaca agaaatgaaa agattacacc agttagggca acgacgggaa gccgagaaag
      781 tgaaaaagaa ctggcgcttt cttctaaaaa accgtcaaaa aatcaatcac tatgattaca
      841 aaacatggcg cagttttcgt gcacctaaat acccgctact cactgaagca atgatgattg
      901 atcgattgct ggcattttca acgcctttaa aggacgccta tacttttttc catgagctga
      961 ccgaagcctt tagagacaag gaatcagact tattttttac ct
//
LOCUS       DAFGZE010000241          981 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.250, whole genome shotgun sequence.
ACCESSION   DAFGZE010000241 DAFGZE010000000
VERSION     DAFGZE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.250"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(31..104)
                     /locus_tag="LTU54_003200"
     tRNA            complement(31..104)
                     /locus_tag="LTU54_003200"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(68..70),aa:Ile,seq:cat)
     gene            complement(659..973)
                     /locus_tag="LTU54_003201"
     CDS             complement(659..973)
                     /locus_tag="LTU54_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297821.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          363 a          154 c          154 g          310 t
ORIGIN      
        1 aacacagaac gattcaattg gctacattaa tgtaccctgt aggactcgaa cctacgaccg
       61 gacggttatg agccgtctgc tctgaccaac tgagctaagg gtagtggttg ttgccacata
      121 aagccataaa caatcaacca gtagaatgcg tggcaacaaa cctgttatcg catatcttgg
      181 agtgtgacta tttatgagtg atagtgaaga tatgcgtctc catatatgta tggttgagac
      241 agataaacat gtatgaaaaa cgttgttaaa tcaacgtttt tttattttgg tcaaggagag
      301 aaaagtagag aaatacaaaa ttttttgcat tgattttgca ttcaaaaaaa cattcaaaca
      361 tttagaaatc cccaatacgt attcaggaaa atctatttta aggtttacaa aaagaacttt
      421 tgttcgtaca ctctttttga ggtgaacttt tatgctgatg gaaggaaaaa cacaattatg
      481 gtttaagcat gttccttcaa ataagttttt taattataaa ctctagagaa attaattcat
      541 cctaacataa aataattgtt cactcaaaag tttttctaga acaattaaaa cagcaggctt
      601 cacattcgct atacatttgt aagaagtaat aattcctgta ctaaaaggca taattgcttt
      661 aattattttt tgacttctct tcaaacacaa gaagtagttg tgtaataatt gcagaaataa
      721 ggattccaat aatagatata aaatttgaca tggaaacgtc tataacatta aaagaataaa
      781 gtgttgaaga aagtaaactg attgaaataa cccctccgta aaaaatagtc tgtgaatata
      841 cccacttttt ttcagaagac gtacttcgtt tggttctaaa acctatccct gcatttctac
      901 tcttatcaaa aaaccaaata tttagaagta ataatattgc tattattaag ataaaattac
      961 tgtaaaaatc caaaagctat c
//
LOCUS       DAFGZE010000242          962 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.251, whole genome shotgun sequence.
ACCESSION   DAFGZE010000242 DAFGZE010000000
VERSION     DAFGZE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.251"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(239..>962)
                     /locus_tag="LTU54_003202"
     CDS             complement(239..>962)
                     /locus_tag="LTU54_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297822.1"
                     /translation="KKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNIIE
                     EIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAID
                     NVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPPT
                     WKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKKD
                     YSVVFKNGQVTDYNEVFKKVIE"
BASE COUNT          308 a          177 c          123 g          354 t
ORIGIN      
        1 atatacaaat ttcttaaaaa ttttttcact aaaatcacta cctgaaacag tctaaaacac
       61 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
      121 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
      181 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
      241 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
      301 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
      361 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
      421 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
      481 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
      541 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
      601 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
      661 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
      721 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
      781 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
      841 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
      901 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
      961 tc
//
LOCUS       DAFGZE010000243          946 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.252, whole genome shotgun sequence.
ACCESSION   DAFGZE010000243 DAFGZE010000000
VERSION     DAFGZE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.252"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            49..825
                     /locus_tag="LTU54_003203"
     CDS             49..825
                     /locus_tag="LTU54_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBL8297823.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
BASE COUNT          357 a          126 c          154 g          309 t
ORIGIN      
        1 tttaataaat tgtgatataa tgaagtataa ctttttaggg agtgtactat gaattttcaa
       61 gataatagac cattatattt acaagtagaa gcttctatac gtaacgatat tcgtgaaaaa
      121 aaatatcttc caggagagca attacctaca gaagaagaat tatgtaaaat ttatggagta
      181 agtaagatta ccattcgaaa agcttttaaa cttttagcag aaagtggaac agttgaacga
      241 ttaaggggga aagggacctt tgtaactcaa aaaaaagaaa gtttaaaatt agctggagtt
      301 catggattta gcgattctct ttctacccga ggacataaaa ttagatatac tgttttacat
      361 tctaaaatta taaaggctga tgagttttta gctaataaat tacaaatcaa tgtaactgat
      421 agagtttaca atattaaacg tttaatgtgg gaagacggtg ccccgatggg aattgataat
      481 tttttcgcat cagaagaaaa atttccaact ttatttgaaa aaggtagcca atatacttca
      541 ttatataaac ttctagaaga tgactatgat gtcaaagcta cgagctctat tatggaaata
      601 aacggtttat ctgcaacacc tgagctttcc gacttattgc aatgtatagt aggagatccg
      661 ctatttattt tagaaaaggt tggcaaagat aaaaataatc aaccatttca ttattctcaa
      721 tccacagttc gttgtgatag aattagttat gttattgaag tcacagaaaa tatcacttta
      781 gatacggtgc ataaaaatga ttcagatcct aaagtaatag attgatatta aaagaaatca
      841 tacccaaagc aatcaatttt gattagattt gggcatgatt tttattttta ctaatattct
      901 ctaaatacat caaaaaactt atttatattc caaatttcac ctgtac
//
LOCUS       DAFGZE010000244          943 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.253, whole genome shotgun sequence.
ACCESSION   DAFGZE010000244 DAFGZE010000000
VERSION     DAFGZE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.253"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..258
                     /locus_tag="LTU54_003204"
     CDS             <1..258
                     /locus_tag="LTU54_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297824.1"
                     /translation="KGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            260..397
                     /locus_tag="LTU54_003205"
     CDS             260..397
                     /locus_tag="LTU54_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBL8297825.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            434..727
                     /locus_tag="LTU54_003206"
     CDS             434..727
                     /locus_tag="LTU54_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297826.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            724..>943
                     /locus_tag="LTU54_003207"
     CDS             724..>943
                     /locus_tag="LTU54_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HBL8297827.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGN"
BASE COUNT          350 a          134 c          201 g          258 t
ORIGIN      
        1 aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt atacgaaacg
       61 ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct aaacaatgta
      121 gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc gcagttgaat
      181 tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat caaaaaacaa
      241 ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt atgactgggg
      301 ctgtttcact aacgaacaag taatggtttt cgttccatta tgcattacag acgaagaagc
      361 agataaaatc attaataaag ataagagcgc atcttaattg atgcgttttt atttaaggta
      421 aaggagttgt cacatgatta atttagggga atggggaatg atagcaggat caataaccgc
      481 tatcgtttct ttgattttat tagtaattag accgattgct gcatctttct cgaaaattac
      541 tgagactctt tcaaaagtaa gccacaattt agatttgctg actaaagatt tagaatcgag
      601 caaatcagat cgattgatga ttcatgaaga actaaagaaa cacgatgaaa gattagatac
      661 acatacagaa aaattggtgg aacacacgca acaaatcaaa actttgttta gggagagaag
      721 aaaatgaata ataaaacgtt cgaagtacta aaatggttcg cactggtaat tattcccgca
      781 ctagctactt tcgtggggtt agttggtaaa gcgctcaatt ggcagtacac agatatctgt
      841 gttgtcatca ttactggttt tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga
      901 acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa aca
//
LOCUS       DAFGZE010000245          934 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.254, whole genome shotgun sequence.
ACCESSION   DAFGZE010000245 DAFGZE010000000
VERSION     DAFGZE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.254"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>934
                     /locus_tag="LTU54_003208"
     CDS             <1..>934
                     /locus_tag="LTU54_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HBL8297828.1"
                     /translation="HSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKS
                     YLPKQSKRILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFL
                     GCANPEEVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNEL
                     RKVGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFS
                     TSLKEAYPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAM
                     IYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRIR"
BASE COUNT          314 a          183 c          175 g          262 t
ORIGIN      
        1 cattcaatcg cgaatcatgt cagatataaa attgcttctt tactgactga aaaagtatct
       61 ttatctttta ttgcgaaaaa ttgtcaggta tctttgacca ccgttattcg tacgttgaaa
      121 gagtttaaaa gctacttacc aaagcaatct aagaggattc tccccagagt attgatggtt
      181 gatgaatttc gttcgcacgc ttccatagaa gataaaatga gctttatttg cgcagatggc
      241 gaaacaggac aattaattga tgtcttgcct acgcgtaaat tgcctcgatt aacaagctat
      301 ttcttaggct gcgctaatcc tgaagaagta aaatttttag tcacagacat gaacgccgcc
      361 tacttccagc ttaccaaacg tattctgcca aatgcgaaag tagtgattga tcggtttcat
      421 attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat gaatgaactt
      481 cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttttgcta
      541 aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt tcgagcacca
      601 aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt ttctacgtcc
      661 ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga caaagaccct
      721 gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg ctttcgggaa
      781 aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat ctatccttac
      841 accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg agtatcctac
      901 ggatttaaat catttgagaa catgagaatt agaa
//
LOCUS       DAFGZE010000246          903 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.255, whole genome shotgun sequence.
ACCESSION   DAFGZE010000246 DAFGZE010000000
VERSION     DAFGZE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.255"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..286
                     /locus_tag="LTU54_003209"
     CDS             <1..286
                     /locus_tag="LTU54_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HBL8297829.1"
                     /translation="LFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEE
                     NLDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            279..626
                     /locus_tag="LTU54_003210"
     CDS             279..626
                     /locus_tag="LTU54_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297830.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFAELLKWSQI"
BASE COUNT          332 a          114 c          184 g          273 t
ORIGIN      
        1 tcttttttta ttcaataagt tttatgaggg gcaggaagta agacatatag gagtaactta
       61 tagcagatta gtatacacag attccctaca attagattta tttagtgatc caagaaaaaa
      121 gattgctgaa gaaaatttgg ataggattat tgataagatt agacaaaaat atggatttac
      181 agctattata catgcttcat ctatgttaga aggtgcaaga agtattacga gaagcacttt
      241 aataggagga cacgcaggag gattaggagg tttgcataat gattgaatat aacgatagag
      301 gacgtttaaa atggtcggga ttttatttgt ctgatcatac tgaaagaatc aatgcagaca
      361 atgtacaacg tgctaatcat aatttggcaa aagaacaaat gacgactgaa gagattgcga
      421 aaatactgag aactgctgtc ataaaaaata gacccgtctg cattcaaaaa gaggaacgaa
      481 acgctgaagg ttactatcca gatgatgtag taggaaaaat tattggatat gacgagcttg
      541 gtgtatttgt ggaagaagaa aaagttcact atgatgaaat taggaatgtt tctttcgcgg
      601 aattattaaa atggagtcag atatgaacat tattaaaaaa tacgaattcg gttatattac
      661 gtatgaacag ctaatagagg aattatgggg atatggtcaa caattaatta atgaagtggg
      721 gattgattgc ttttgtttct atattgaatc cggagcagga taccataaga acagatacta
      781 tatatttcca tatccaccaa aatagtctct tagagatttt taaattttct ccaaatactt
      841 tcaattattg tgaaatattt ccctttaaca gaattaggca gaagtcgccc atcctctata
      901 ggt
//
LOCUS       DAFGZE010000247          889 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.256, whole genome shotgun sequence.
ACCESSION   DAFGZE010000247 DAFGZE010000000
VERSION     DAFGZE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.256"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..540
                     /locus_tag="LTU54_003211"
     CDS             <1..540
                     /locus_tag="LTU54_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297831.1"
                     /translation="PKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDE
                     TDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLIS
                     PISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEK
                     ILLPENWAELRKLVEYGII"
     gene            527..>889
                     /locus_tag="LTU54_003212"
     CDS             527..>889
                     /locus_tag="LTU54_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HBL8297832.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLV"
BASE COUNT          307 a          122 c          132 g          328 t
ORIGIN      
        1 cccaaaatag atgaaaacag taaattgatg aaaaacatac aatcaatctt tgggatagaa
       61 gaagtttttg tcggagataa atgtatcttt tttgatcaac ctttattatc tgatggttat
      121 ggcatagatg aaacagatat tttcgctaaa gtaaatagta tattttctgg aaaaaaagaa
      181 actttaatta agcttcatcc tagagcaagg acaaatcgtt atggtgaaaa aaacatattg
      241 aattccagtt taccgtttga agtcttgcta cttgaaaatg acttctcttc aacacttttg
      301 attagtccta tatctacaat ttctttttca ccatctatga tgtttgaaaa gacgatcaac
      361 tctattatgt tggctaaatt aattttaaat gaatatacag cattatctga agataaaaaa
      421 gaaacattga caaatattgt tcttttctgt gaacattata accgtctatc agaagaaaaa
      481 attttactcc cagaaaactg ggcagaatta agaaagttgg tagagtatgg gattatttaa
      541 cacactttcc aagtcaaaat tagttaagtc cagtttctgg tatactatag ggaatttttt
      601 tattaaggga atttcattca taactattcc tatttttgta aatattacta ctcaagcaga
      661 atatggtttg attaataatt tcacttcatt tgtatcaatt ttttcgattc ttgttgggct
      721 aagcctaaat tcggcaatta ataatgcaag ttttgactat aaagatgaca taaatgaatt
      781 tatgtcatcg gtactttctc tagctacact ttcgttaatt gtgttcttat taatagggaa
      841 catatatttt ttgttttcaa atgctttttt tgatatgaac caacttgta
//
LOCUS       DAFGZE010000248          812 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.257, whole genome shotgun sequence.
ACCESSION   DAFGZE010000248 DAFGZE010000000
VERSION     DAFGZE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.257"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            8..81
                     /locus_tag="LTU54_003213"
     tRNA            8..81
                     /locus_tag="LTU54_003213"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Asn,seq:gtt)
     gene            121..210
                     /locus_tag="LTU54_003214"
     tRNA            121..210
                     /locus_tag="LTU54_003214"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Ser,seq:gga)
     gene            225..296
                     /locus_tag="LTU54_003215"
     tRNA            225..296
                     /locus_tag="LTU54_003215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:258..260,aa:Glu,seq:ttc)
     gene            312..384
                     /locus_tag="LTU54_003216"
     tRNA            312..384
                     /locus_tag="LTU54_003216"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:345..347,aa:Val,seq:tac)
     gene            394..466
                     /locus_tag="LTU54_003217"
     tRNA            394..466
                     /locus_tag="LTU54_003217"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:427..429,aa:Asp,seq:gtc)
     gene            502..577
                     /locus_tag="LTU54_003218"
     tRNA            502..577
                     /locus_tag="LTU54_003218"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:535..537,aa:Phe,seq:gaa)
     gene            579..659
                     /locus_tag="LTU54_003219"
     tRNA            579..659
                     /locus_tag="LTU54_003219"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:613..615,aa:Tyr,seq:gta)
     gene            666..739
                     /locus_tag="LTU54_003220"
     tRNA            666..739
                     /locus_tag="LTU54_003220"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:698..700,aa:Trp,seq:cca)
BASE COUNT          178 a          185 c          224 g          225 t
ORIGIN      
        1 atgttattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat gatgtcgtag
       61 gttcgagtcc tactgccgga gttctcattt tgagaaagag tattgagctt ctttcactct
      121 ggagagttgt ccgagaggcc gaaggagcat gattggaaat catgtagatg gtgccaaact
      181 gtctcaaggg ttcgaatccc ttactctccg tagcaaattt acctggcccg ttggtcaagc
      241 ggttaagaca ccgccctttc acggcggtaa cacgggttcg aatcccgtac gggtcatttt
      301 aattgaatgt tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
      361 gcggttcgat cccgttaacc tccataatga actggttccg tggtgtaggg gttaacatgc
      421 ctgcctgtca cgcaggagat cgcgggttcg attcccgtcg gaaccgtcgt tcttgttatt
      481 caattaataa ttttaaaata tggcgcggta gctcagttgg tagagcaacg gattgaagct
      541 ccgtgtgtcg gcagttcgat tctgtcccgc gccaccatgg aggagtagcg aagtggctaa
      601 acgcgacgga ctgtaaatcc gttccttagg gttcagtggt tcgaatccac tctcctccat
      661 tttatagggg catagtttaa aggtagaaca gcggtctcca aaaccgttag tgtgggttca
      721 attcctgctg cccctgccat ggcgaatgtg gcgaagtggt taacgcaccg gattgtggct
      781 ccggcattcg tgggttcgat tcccatcatt cg
//
LOCUS       DAFGZE010000249          809 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.258, whole genome shotgun sequence.
ACCESSION   DAFGZE010000249 DAFGZE010000000
VERSION     DAFGZE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.258"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..339)
                     /locus_tag="LTU54_003221"
     CDS             complement(<1..339)
                     /locus_tag="LTU54_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297833.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
     gene            complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LTU54_003222"
     CDS             complement(356..760)
                     /gene="tnpA"
                     /locus_tag="LTU54_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HBL8297834.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          244 a          131 c          132 g          302 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgccaccag
      421 ttgtcagaag acaaaagctt ttagaccaga acatttcttt ccaaagaaat tgtttgactc
      481 ttggaaagtc acgttttatc aatcttgagc tggcactttt ataggcattg atgaattttg
      541 tcaattctgt tttaggttga gctttgaaca taatatgaac gtggtcttta tcatgattcc
      601 actccactaa agtaatgtga tacgattctg aaattctttc aaaagtagtt ttggcataat
      661 cagatatttc atcatcaatc acttgtctac gatatttcgt tactaaaaca aggtggtaat
      721 aaagaagaaa tactgaatga ttatttgtat ctaattccat gttatatcaa ctcatttctt
      781 gtatagactg attgtaacat gggacaaaa
//
LOCUS       DAFGZE010000250          805 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.259, whole genome shotgun sequence.
ACCESSION   DAFGZE010000250 DAFGZE010000000
VERSION     DAFGZE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.259"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            6..>805
                     /locus_tag="LTU54_003223"
     CDS             6..>805
                     /locus_tag="LTU54_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HBL8297835.1"
                     /translation="MLINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAGFNS
                     GNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQKGI
                     TMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHIPLK
                     RQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLVVTD
                     GLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          274 a          117 c          149 g          265 t
ORIGIN      
        1 tggaaatact aatcaataaa ggtgatttgg atgatttgtt tcgtcgtcac ctagaattgg
       61 ctattaacgc tttattacaa gctgaattaa cggtatttct tgactatgaa aagtatgata
      121 gagctggatt taattcaggt aattcccgta atgggaatta ttcgcgctct tttaaaacag
      181 agtacggtga attgaattta gtaataccta gagataggaa tggagacttt tctcaacaaa
      241 ctttacctgc ttataaaaga accaatgact ctttagaaac cactattatt cagctagttc
      301 aaaaaggaat tactatgtcc gaaatctctg atctaatcga aaaaatgtat ggtcattatt
      361 acaaaccaca aactatttca aatatgagta aaattgtgtc tgaagatgtt tcggcattta
      421 aagaaagaac tttagaagct aaatactcag tcatttttat ggatgctact catattccct
      481 taaaaagaca aaccgtatca aaagaagctg tctatatagt aattggtatc aggttggatg
      541 gaactaaaga ggttctagga tttactatag ctccaactga atctgcttat gtttggaaag
      601 agattcttca agatctaaaa gaccgtggtt tagaagaggt tttgttagtc gtaacagatg
      661 gtttaagtgg tattgacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat
      721 gttgtgtcca tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa
      781 tctgtagcga tttcaaatta gttta
//
LOCUS       DAFGZE010000251          793 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.260, whole genome shotgun sequence.
ACCESSION   DAFGZE010000251 DAFGZE010000000
VERSION     DAFGZE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.260"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..782
                     /locus_tag="LTU54_003224"
     CDS             <1..782
                     /locus_tag="LTU54_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8297836.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDSYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          341 a          107 c          101 g          244 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatag ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc ttt
//
LOCUS       DAFGZE010000252          790 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.261, whole genome shotgun sequence.
ACCESSION   DAFGZE010000252 DAFGZE010000000
VERSION     DAFGZE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.261"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..640)
                     /locus_tag="LTU54_003225"
     CDS             complement(<1..640)
                     /locus_tag="LTU54_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBL8297837.1"
                     /translation="MNSSDLISVIIPVYNVEKYVGSCLESVCNQTYKNLDIIIVDDGS
                     KDSSNKICEDYALKDSRIRIIHKNNAGLGLARNTGLEHVKGKYVAFIDSDDFVAANYI
                     EAMLNGIKRSNADTCYAGYYEYYDDNNIISKPARYDSTIFTNNAIIEKVLLGMIGTEP
                     SYKVDFILPMSVWHALYSSEIIQQHNIKFPSERDYISEDIIFHISYLQHSKIT"
BASE COUNT          258 a          150 c           87 g          295 t
ORIGIN      
        1 ttgttatttt tgaatgttgc aaatatgaaa tatggaatat gatatcttca gatatataat
       61 ccctttctga cggaaacttg atattgtgct gttgaattat ctcagatgaa tacagcgcat
      121 gccaaactga cattggcaat ataaaatcga ctttataact cggctcagtg ccaatcattc
      181 caagcaacac tttttcaatt atagcgttat ttgtaaatat cgtactatca tatcttgctg
      241 gctttgaaat aatattatta tcatcataat attcataata tccagcataa catgtgtctg
      301 cattactcct ttttatacca tttaacattg cttcaatata attagctgca acaaaatcat
      361 cagaatcaat aaaagcaaca tattttcctt ttacatgttc aagtcctgta tttcttgcca
      421 atcccaagcc agcattattt ttatgaataa tacgaatcct tgaatccttt aatgcatagt
      481 cttcacatat tttattagat gaatccttag atccatcatc cacaattata atatctaggt
      541 tcttatatgt ttgattacac actgattcca aacaacttcc tacatacttt tcaacattat
      601 atacaggtat gattacacta attagatcac tcgaattcat ccttctatcc tttcactcac
      661 gttttctcaa ttagtcaatg acggcatcaa cgattttctt aaattctctg aaaaaaatag
      721 actctgatct tgatgatgtg aatgacatac tatcttcttc tagaaaaatt atttccttta
      781 aattatttat
//
LOCUS       DAFGZE010000253          779 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.262, whole genome shotgun sequence.
ACCESSION   DAFGZE010000253 DAFGZE010000000
VERSION     DAFGZE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.262"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..110)
                     /locus_tag="LTU54_003226"
     CDS             complement(<1..110)
                     /locus_tag="LTU54_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297838.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            191..766
                     /locus_tag="LTU54_003227"
     CDS             191..766
                     /locus_tag="LTU54_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBL8297839.1"
                     /translation="MKSHLWVTQNIWGDHTDPCGYLSEMGVSKEKLAYDLAHGFTDDN
                     PTTSDDNPTTSEDKPVIDPTRAGAANSTLTDGTNYAHVDQFGEIENANLHVAGWHIAN
                     YKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYV
                     MMRATNDPEGNTKGGAQDFHDKRWYLNIPQR"
BASE COUNT          249 a          141 c          151 g          238 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccacagga atcaaatcgc atttatgggt aacacaaaat atttggggcg atcatacaga
      241 tccttgcggt tatctttctg aaatgggtgt aagtaaagaa aaactagcct atgatttggc
      301 tcatggtttt acggatgata atccaactac ttcggatgat aatccaacta cttcggagga
      361 taaaccagta attgatccaa ctcgagcagg tgctgcaaat tctacgctga cagatggaac
      421 aaattacgcc cacgttgatc agttcggaga aatcgaaaac gcaaacttgc atgtagctgg
      481 atggcacatt gctaattata aatacgagta tattttcatt atggactaca atactggaaa
      541 agaactagct agagtaagag ctgatgggat ttatagacca gacgtaaatc aagcttataa
      601 tactttagga aatgttggct atcatgtatc tttcaatatg cgtgatttcc ctaataagaa
      661 agtatacgtc atgatgcgtg cgacaaacga tccagaagga aatactaagg gtggagcaca
      721 agattttcat gacaagcgtt ggtatttaaa tattccacaa cgataaaaaa ttcccccct
//
LOCUS       DAFGZE010000254          768 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.263, whole genome shotgun sequence.
ACCESSION   DAFGZE010000254 DAFGZE010000000
VERSION     DAFGZE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.263"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            23..>768
                     /locus_tag="LTU54_003228"
     CDS             23..>768
                     /locus_tag="LTU54_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HBL8297840.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQH"
BASE COUNT          277 a          143 c          133 g          215 t
ORIGIN      
        1 tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc aaactctagt
       61 cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga taaacacact
      121 cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca ctggttttaa
      181 ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat atggtgaatt
      241 aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt taccagccta
      301 caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa aaggtgttac
      361 gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata ctccacaaac
      421 catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat ccagagcctt
      481 aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa aacgtcaaac
      541 cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca ctaaagaagt
      601 actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc tgctacagga
      661 tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct taaaaggcat
      721 gaaagatact atccatcaaa tttatcctaa agcaaaatat cagcattg
//
LOCUS       DAFGZE010000255          752 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.265, whole genome shotgun sequence.
ACCESSION   DAFGZE010000255 DAFGZE010000000
VERSION     DAFGZE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.265"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(91..>752)
                     /locus_tag="LTU54_003229"
     CDS             complement(91..>752)
                     /locus_tag="LTU54_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HBL8297841.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          204 a          176 c          144 g          228 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgattc gaccgattcg gc
//
LOCUS       DAFGZE010000256          723 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.266, whole genome shotgun sequence.
ACCESSION   DAFGZE010000256 DAFGZE010000000
VERSION     DAFGZE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..723
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.266"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            69..689
                     /locus_tag="LTU54_003230"
     CDS             69..689
                     /locus_tag="LTU54_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297842.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
BASE COUNT          266 a          110 c          162 g          185 t
ORIGIN      
        1 ttgatcatcg caaccgttga tgatccaaag caaaatgtgt tcactcttga gaaagtcggg
       61 gtgatctaat ggctaaaact aaacgaatga tggaaacgga tgaaaaaaca ggtgtacaac
      121 gccaattctt tccaatcaca catgcttccg cagttcttgg gttagaagaa ataatagcag
      181 gagaagcaac agttttatct gttaatggaa aaattggagc cgtcgtcatt actaaagaag
      241 atttgggatt agagaatgtt ctcacagaat taccctatgc aagtgaagaa gatgacggca
      301 ttatcacggc agaaatgtat caaaaaattt taaacagtgg agaaggtgac tacgtgttgc
      361 cagttgccac tatcgaccgt ttgggtggca taaaagttgg tgaattattg acgattgatg
      421 agacagggaa agtctctgca gtcagacaat ctgatgtcaa tttttcgctg gagttaaaag
      481 aaaaactcga ttcactgaaa aattatactg ctggagaaaa catcactatt gatgaagatg
      541 gaacaattag ttctacaggt ggtagtggga ctggtggtgt caatcagtca tatgtagatc
      601 aaaaattcca agaagctgta aatcaagcag aaaattacac aaacgaaaga attccaaatt
      661 ttacttttga aaaaattggg gaggtataga gaatgacaga tatcgtaaaa ataaaacaaa
      721 gca
//
LOCUS       DAFGZE010000257          716 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.267, whole genome shotgun sequence.
ACCESSION   DAFGZE010000257 DAFGZE010000000
VERSION     DAFGZE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.267"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>716
                     /locus_tag="LTU54_003231"
     CDS             <1..>716
                     /locus_tag="LTU54_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBL8297843.1"
                     /translation="YVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQ
                     VSDTVANLHGQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVV
                     TYGAKFLKVDSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDV
                     ANAAILKSDKEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRIT
                     IKNNQKASVPMTGSNGFQSYV"
BASE COUNT          283 a          110 c          141 g          182 t
ORIGIN      
        1 tatgttgctg aaaaagcaga tattaaagta acaaataatg atggttcagt cgtattaaaa
       61 gaaaatactg actataaaat cgaaaattct gatagtggtt ttgtgatcga ttttatcgta
      121 aataatggac aagtaagtga tacagttgcg aatttacatg gtcaagatct aaaaatgact
      181 tataacatgt ttgtgaatga tagtgctgaa atcgcaaatc cattaacaaa tagcgtggat
      241 ttcgtttata acaataatcc atttaatcag gaagaacacc acgaaaaaac gaaagctgac
      301 gttgtgactt atggcgcaaa attcttaaaa gtcgatagcg gcctatttgg aacaggtatc
      361 aaagcaacac cgcttgaagg cgcagaattt gcagctaaga atgcagaagg taagtactac
      421 ggtggactta ctgatacaga taaagatggt gtcaaagaag cagtttgggt agacgatgta
      481 gcaaatgcag caatcctaaa atcagataaa gagggtcatt ttgaaatcac tggtttaact
      541 gaaggagatt actcactgga agaaacaaaa gctcctgaaa actatcaaaa attaacaaaa
      601 gagatctctt tcaaagtaga caaagattca tacaaagaag aaaaccgaat cacgatcaaa
      661 aataatcaaa aagcttccgt accaatgact ggtagcaacg gtttccaatc ttacgt
//
LOCUS       DAFGZE010000258          706 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.268, whole genome shotgun sequence.
ACCESSION   DAFGZE010000258 DAFGZE010000000
VERSION     DAFGZE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.268"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            120..>706
                     /locus_tag="LTU54_003232"
     CDS             120..>706
                     /locus_tag="LTU54_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297844.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVA"
BASE COUNT          235 a           92 c           91 g          288 t
ORIGIN      
        1 aaacactact tttgtaatat aaaaaatatt ttattaataa taaaattaat cacattttat
       61 atagtgacta atctagtgtt ttaaaaaaat aaatgatact atatgtaata taaaaaaaca
      121 tgaggtggta tatgaatttg aaaaaaattt tttctgtaaa aatatttata gtttatgcat
      181 ttgttagttt attatattta tttccagcat ttaaaaacat ggaaatattt ggcttttatg
      241 atacggcatt tcacattaat cgtgcgttaa gtttagaatc tatttttagc tcccctatta
      301 attttgaaac gtttagatca tatggcatgc aagtaaataa tttttaccca tggttaactc
      361 tatatccttt atttttattg atcaagttta ctaatttagc aataggctat aacctttttc
      421 tttatattgt tacgctcatt actctattta tatgtcatta tgtaatgtat gaaattacca
      481 aaaaacatgt tactagctcc tttttcgcta ttatatatac aacatcttct tttaggagtg
      541 tagaaatatt tcttcgaggg gcaatggggg aattactcgc aatgtcaata cttcctctta
      601 ttttactcgg gtttataaag ctttacgact caaaaaaaga atcttgggtt atgttggcga
      661 ttagcatgac tttattaatt tacactcatg ttctttctgt agctat
//
LOCUS       DAFGZE010000259          700 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.269, whole genome shotgun sequence.
ACCESSION   DAFGZE010000259 DAFGZE010000000
VERSION     DAFGZE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.269"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            73..690
                     /locus_tag="LTU54_003233"
     CDS             73..690
                     /locus_tag="LTU54_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297845.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          253 a           96 c          136 g          215 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccgagaa ccttgggttt tcaagtgctt tttggttcat taatcttaac catagtgttt
      121 ttaaagtgga aaacaagcaa caaaaaaagt atgtggagcc ttgtaggaat agttcttatt
      181 atttttactg tgactgcagc aacagaagca aattattcgc ataaaattca gtcgtataaa
      241 ggtcgtttag ttatgaccaa aaaagatgtt acacgttata catcctcaag aactggaggc
      301 ctatatgact atgcaccatt agatgcattg aaatataaag atcatttaaa gaaacatgaa
      361 gtaaaagttg gtgatcggtg ggagaatgca tctcataaag catataattc caaaatagtc
      421 tatacagtag aagatgccga tgagcaaaat atcacattac ctgtattcaa ttattgggga
      481 acagttgtga aagtaaatgg gaaaaagagc actatagaaa atgacaatgg tttagtgtct
      541 attaaaggta cggggaaaag aactatagtt gaggtttcat ctacgtatcc aactacgatg
      601 attctatctt tattaattag ttcaataact tttatattct tatttacacg ttatttaata
      661 agatgctgcg gtgaaatgaa aatagattag atagatatta
//
LOCUS       DAFGZE010000260          697 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.270, whole genome shotgun sequence.
ACCESSION   DAFGZE010000260 DAFGZE010000000
VERSION     DAFGZE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.270"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..667
                     /locus_tag="LTU54_003234"
     CDS             <1..667
                     /locus_tag="LTU54_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HBL8297846.1"
                     /translation="GKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYR
                     WVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWLRKK
                     RDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNL
                     IEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLG
                     IPA"
BASE COUNT          239 a          122 c          139 g          197 t
ORIGIN      
        1 aggaaagcaa tttcagcagg atgtgattat tgtagccgtg ggctactatc ttcgttataa
       61 ccttagctat cgtgaagttc aagaaatctt atatgatcgt ggcattaacg tttctcatac
      121 gacgatttat cgttgggtgc aagaatatgg caaactactc tatcaaattt ggaaaaagaa
      181 aaataaaaaa tccttttatt catggaaaat ggatgaaacg tacatcaaaa ttaaaggaaa
      241 atggcattat ttgtatcgag ccatcgatgc agatggttta accttggata tttggttacg
      301 taaaaaacgg gacacacaag cagcctatgc ttttcttaag cggttagtga agcagtttga
      361 tgaaccgaag gttgtagtca cagataaagc cccctctatt acaagtgcct ttaagaaact
      421 aaaagaatac ggcttttatc aagggacaga acatcgtacc attaaatacc tgaataattt
      481 gattgaacaa gaccatcgtc cagtaaagag acgcaataaa ttctatcgaa gtttacgcac
      541 tgcctctacc acgattaaag gcatggaagc cattcgagga ttatataaga aaacccgaaa
      601 agaaggcact ctcttcgggt tttcggtctg tactgaaatc aaggtattat tgggaatccc
      661 agcttaaatc atagataccg taagggattt tattctt
//
LOCUS       DAFGZE010000261          654 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.271, whole genome shotgun sequence.
ACCESSION   DAFGZE010000261 DAFGZE010000000
VERSION     DAFGZE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.271"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..525
                     /locus_tag="LTU54_003235"
     CDS             <1..525
                     /locus_tag="LTU54_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297847.1"
                     /translation="KMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLH
                     EKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQ
                     YKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASIN
                     ILTEGLRLHSMGLA"
BASE COUNT          244 a          105 c          126 g          179 t
ORIGIN      
        1 aaaatggaaa agaaactaaa gcgtgaacaa cgcaaattgt ctaggcgtgc tttattggct
       61 aaaaataaag gtaccaatct atttgaagct aaaaattatc aaaaacaaaa acgcaaagta
      121 gctagattgc atgaaaaagt aatgaatcaa cgtactgatt ttctgaataa gttgagtaca
      181 gaaatgatca aaaatcacga tattatctgt attgaagact taaacacaaa aggtatgttg
      241 cgaaatcata aattagctaa aagtatttct gatgtttctt ggtcaaagtt tgtgactaaa
      301 ttacaataca aggctgactg gtatggacga aaaatcatca aagtagataa atggtttccg
      361 tctagtcaga tttgttcaga gtgcggacat aaagatggca agaaatctct tgaaattcga
      421 gaatggactt gtcctgtttg tcatactcat catgatcgtg atatcaatgc tagtatcaat
      481 attctgaccg aaggtctaag actacattct atgggattgg cttagatcca atcatcaaga
      541 accgtaggaa ctacggggat agcttggtaa ataagagaca ccgctgttag taaagaaata
      601 cgctatcaag tatgctctat tcccaagaag ctcccacttc aagcgttaag aacc
//
LOCUS       DAFGZE010000262          645 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.273, whole genome shotgun sequence.
ACCESSION   DAFGZE010000262 DAFGZE010000000
VERSION     DAFGZE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.273"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            221..619
                     /gene="spxA"
                     /locus_tag="LTU54_003236"
     CDS             221..619
                     /gene="spxA"
                     /locus_tag="LTU54_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HBL8297848.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          230 a           95 c          122 g          198 t
ORIGIN      
        1 tttgaattag atttgaaaac tctaattttt aaatgataaa aaaagcgaga atgctggttt
       61 tactgtatat taaccttaga taactttcat ttttttaaaa aaattatgaa aaaaaggtag
      121 ctatttaaaa gttgattatg tataatagaa acagatggga gattattatg atcttctgtt
      181 acaaaaaagt gattcttatc acgaaaagga gtggtgcacg ttgttaacat tatatacttc
      241 cccaagttgt acttcctgca gaaaggctcg tgcgtggttg caagaacatc agattccatt
      301 tgttgaacgt aatatttttt cagaaccatt gaacagttct gaattaaaag cgattttaca
      361 aatgacagaa gacggaacag aagaaatcat ctcgacacgt tccaaagtat tccaaaaact
      421 gaacatggat ttagatgatc tgccgttaca agaattattg gaacttgtgc aaaacaatcc
      481 tggattgctt cgccgtccaa tcatgattga tgataaacgt ctgcaagttg gttttaatga
      541 agatgaaatt cgtcggttcc taccgcgcga tgtacgacaa ttagaattac gtcaagcaca
      601 attgatggct ggattataga aggataatac gaaacttgga aaaga
//
LOCUS       DAFGZE010000263          643 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.274, whole genome shotgun sequence.
ACCESSION   DAFGZE010000263 DAFGZE010000000
VERSION     DAFGZE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.274"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..594
                     /locus_tag="LTU54_003237"
     CDS             <1..594
                     /locus_tag="LTU54_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBL8297849.1"
                     /translation="GEDNIEKINNNLTILDTALENKEQKISILNSQLSSNNQTLEQLR
                     TNIEVSKQKVASLENDKNQLVSEKTNLENQLSAKDSELQGKQNEINAKIDEINQKVAE
                     INRLNSDWNNRLQNEKNKFNEEQSTINSLNQQINQMLSEKNNIQNQLQATQQKLEEEK
                     SKNSSLNNYIEKLQKAKNDVEDTANKAQQIVNEHTIQ"
BASE COUNT          313 a           81 c           93 g          156 t
ORIGIN      
        1 ggggaagata atattgaaaa gatcaacaac aatttaacta tattagacac agctctagaa
       61 aataaagagc aaaaaattag tattttaaat tcacaattgt cttcaaacaa ccaaactctt
      121 gaacaactaa gaactaatat tgaagtttcc aaacaaaaag tggcatcttt agaaaacgac
      181 aagaatcaat tagtatcaga gaaaactaat ttagaaaatc aattgtcagc aaaagactct
      241 gaattgcaag gaaaacaaaa tgaaattaat gcgaaaatag acgaaattaa ccaaaaagta
      301 gctgaaataa atcgattaaa tagcgattgg aataatcgtt tacaaaatga aaagaacaaa
      361 tttaatgaag aacaatctac tattaatagt ttaaaccagc aaattaatca aatgttgagt
      421 gaaaaaaata acattcaaaa tcaactgcaa gctactcagc aaaagctaga agaagaaaag
      481 tcaaaaaata gtagtttgaa taattacatt gaaaagcttc aaaaagctaa aaatgatgta
      541 gaggataccg caaataaagc acaacaaatt gtaaatgaac atacaatcca atagatatag
      601 ttttaattta acaaatggta tgtttggaga taatgagata gaa
//
LOCUS       DAFGZE010000264          642 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.275, whole genome shotgun sequence.
ACCESSION   DAFGZE010000264 DAFGZE010000000
VERSION     DAFGZE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.275"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(14..400)
                     /locus_tag="LTU54_003238"
     CDS             complement(14..400)
                     /locus_tag="LTU54_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297850.1"
                     /translation="MKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRFEVLKIQKNE
                     LLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTDVVMITDIYV
                     EDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          200 a          122 c           78 g          242 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg ttcctttatt tttattaaca agttttatca
       61 caggcttcat atatttgatt atatcttcat cttctacata gatatctgtt atcattacga
      121 catctgtact atttcgattg agtactttca cttgatctcc agcattgatt ttccctctaa
      181 atcttttagg aatgaaccaa ctatacatat gattagcatc accaaaatgt gcttttatcg
      241 cattctcagg atataactta ttcaatagtt cattcttttg aattttcaaa acttcaaatc
      301 tatacttatc atcttctgct aatctaaata aatcttctaa acgcatgttt tctaactgta
      361 agttttgaaa atcttccggt ataaattttc tatatttcat taataatgaa cgcccagccc
      421 gatttattag aatctggcca ttttggtcat ctctataaag aattccgtaa cccatcaacg
      481 ccaatagcac acgtgacgtt ttgttacttg taatattcat atttccttga ttacctgagg
      541 caatgaaaga cttatctact tgaattaggg tgaaattctc attcaaatat ggtagtaaaa
      601 ttaatatatg tgaaaatcga atcttgagat agctattccc ct
//
LOCUS       DAFGZE010000265          638 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.276, whole genome shotgun sequence.
ACCESSION   DAFGZE010000265 DAFGZE010000000
VERSION     DAFGZE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.276"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LTU54_003239"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="LTU54_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBL8297851.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..>638)
                     /locus_tag="LTU54_003240"
     CDS             complement(481..>638)
                     /locus_tag="LTU54_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297852.1"
                     /translation="NEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNYILQAILK
                     ELLSNAL"
BASE COUNT          190 a          140 c          107 g          201 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttc
//
LOCUS       DAFGZE010000266          624 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.277, whole genome shotgun sequence.
ACCESSION   DAFGZE010000266 DAFGZE010000000
VERSION     DAFGZE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.277"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            73..234
                     /locus_tag="LTU54_003241"
     CDS             73..234
                     /locus_tag="LTU54_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBL8297853.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..>624
                     /locus_tag="LTU54_003242"
     CDS             253..>624
                     /locus_tag="LTU54_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBL8297854.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNN"
BASE COUNT          257 a           61 c          108 g          198 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taat
//
LOCUS       DAFGZE010000267          623 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.278, whole genome shotgun sequence.
ACCESSION   DAFGZE010000267 DAFGZE010000000
VERSION     DAFGZE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.278"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(138..>623)
                     /locus_tag="LTU54_003243"
     CDS             complement(138..>623)
                     /locus_tag="LTU54_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297855.1"
                     /translation="LNYSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKD
                     QLLLKNHQVTEDGQVIYSSFSPNKDEITFTLKSSKGKTIVLPVFRYKGLTVTVNSKNA
                     TVLKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSH
                     E"
BASE COUNT          210 a           90 c           80 g          243 t
ORIGIN      
        1 aaacagaaca gaaagaggtg ttcttctttc tgttctgtcc aaattattct aagatttctc
       61 tttaccaaca ttatttgaca aagggcctat taaatctaaa atagtatctt tagattgcta
      121 aaatatgtgc tagcaaatca ttcatggctt gttcttattt ttaattttat gcaataaaaa
      181 agaagaacaa ttagcgtgat tatagaaata aataatgaga ttttagaaaa aaatgtgtat
      241 gaatacctga ttgttacttt atttattcca ctttcacttt ttactgtaac aaaaggacct
      301 ccttgtttta gtactgtagc attttttgaa ttaacagtaa ccgttaatcc tttataccgg
      361 aatacaggta aaactattgt tttgcctttt gaagatttaa gcgtaaatgt aatttcatct
      421 ttatttggag aaaagctact ataaataact tgaccatctt ctgtaacttg atggtttttc
      481 aaaagcaatt gatctttttt atagcctata ggtacataat caaaattctc aaattcttca
      541 agtttatttt ccatttgttc attagaataa gttccttgaa aattcatttc tttcaccatg
      601 ttaactactg atgaataatt taa
//
LOCUS       DAFGZE010000268          616 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.279, whole genome shotgun sequence.
ACCESSION   DAFGZE010000268 DAFGZE010000000
VERSION     DAFGZE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.279"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..220
                     /locus_tag="LTU54_003244"
     CDS             <1..220
                     /locus_tag="LTU54_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBL8297856.1"
                     /translation="KFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKY
                     FFENVVTGKENWNDLYHANEVLKIAEEK"
     gene            221..>616
                     /locus_tag="LTU54_003245"
     CDS             221..>616
                     /locus_tag="LTU54_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HBL8297857.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLK"
BASE COUNT          240 a           68 c          113 g          195 t
ORIGIN      
        1 aaaatttcaa ttgtttactt tagatgaatg tatcaatgtt gacttattaa ataataaaat
       61 aagttttgaa gaaaacattg ttaaagaaat gcctgaatct gctaatgata tgtatgagaa
      121 ggagttgaaa tatttctttg aaaacgtagt aacaggcaaa gaaaactgga atgatttata
      181 tcatgcaaac gaagtattga aaatagctga ggagaaataa atggaaattt tatttactat
      241 ttgtggtaga gctgggtcta aggggtttaa aaataaaaat ttgaaatatt tggcaggaaa
      301 gcccctagtc catttcgctt tagcaatgat agatatctat caacaaaaac atcctgaaca
      361 taattgcacg acttgtttga atacggacag taatgagtta gtagaattga cgactaagtt
      421 aaatccgtct gtgaaaattg tagaacgtaa acctgaatta gctacagata ctgtagggaa
      481 aattgaagtg atacgtgatt cattcttaca actagaaaga gaagataata tatttgattt
      541 aattatagat ttagatttga cttcaccttt acgtgaagtt tctaatcttg aagaattaat
      601 agatgaacat ctaaaa
//
LOCUS       DAFGZE010000269          603 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.280, whole genome shotgun sequence.
ACCESSION   DAFGZE010000269 DAFGZE010000000
VERSION     DAFGZE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.280"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            288..>603
                     /locus_tag="LTU54_003246"
     CDS             288..>603
                     /locus_tag="LTU54_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297858.1"
                     /translation="MNENGGFEESKKYQATSEEEKAAVRELLEEAAPTDSLKEKEKDH
                     FYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQI"
BASE COUNT          242 a           93 c          137 g          131 t
ORIGIN      
        1 tgggctataa caagtacggc ggactggata tgacgatcga taatattcca agaatcaaag
       61 aagaaatcga acgctttgaa aaaggtgaat cgactttttc agccgccacg atccgaaaat
      121 atcaacggaa gttggaaaca ttagaacagc tgaaagaaaa atcagaaaaa ggaaaagaaa
      181 atatcctccc tgaagtacaa gcgatcattg acagcggacg agtgacgcaa tggaaaaaaa
      241 atccaaccat ttattttttg aaaggctatc gaaaagttgc tttagaactg aatgaaaacg
      301 ggggatttga ggaatcaaaa aaataccaag ccacgtcgga agaggaaaaa gcagctgttc
      361 gtgaattact tgaagaggct gcaccaactg attctctgaa ggagaaagaa aaagatcatt
      421 tctatcgagt agaattcaac gaaaatgatc cagaaaaagg attgaaaagt tatggtgggg
      481 agatcgtaac attagaattg atcgatcaac ttaaaacgtt ggataaccaa tacgctgcag
      541 aagcaggtta ttataaattc tattttgaag aagtgaagaa cggagaagta gtagatcaga
      601 tcc
//
LOCUS       DAFGZE010000270          602 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.281, whole genome shotgun sequence.
ACCESSION   DAFGZE010000270 DAFGZE010000000
VERSION     DAFGZE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.281"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..318
                     /locus_tag="LTU54_003247"
     CDS             <1..318
                     /locus_tag="LTU54_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297859.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          241 a           80 c           79 g          202 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggcttt tccatttaca cataattatt
      361 gacacaacct attctggaaa cttacaaata aaagcttatt cttcagccac cttcgggtgg
      421 ttatttttta taaaaaaatg taaaaaaata ttgatacata aataaattag ttgtagtaaa
      481 tagttttaga attgaatttc tctatccttt ttaaataatt ttttagacaa aaaacagaaa
      541 gtgtctaaag aaagcttgac gaagaaaata atttttaata acttatttaa ggaaattttt
      601 tg
//
LOCUS       DAFGZE010000271          597 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.282, whole genome shotgun sequence.
ACCESSION   DAFGZE010000271 DAFGZE010000000
VERSION     DAFGZE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.282"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..508
                     /locus_tag="LTU54_003248"
     CDS             <1..508
                     /locus_tag="LTU54_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HBL8297860.1"
                     /translation="DLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFS
                     RIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLK
                     VEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELG
                     CANASEAV"
BASE COUNT          181 a          109 c          132 g          175 t
ORIGIN      
        1 agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca ttttggatct
       61 gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg ttctagtaaa
      121 aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc atactgatcg
      181 tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg acatcaatcg
      241 atcattgagt cataaaggct gtccttttga taatgccgta gctgaatcaa cttataagtc
      301 gttgaaagta gaatttgtct atcaatacac atttgaaacc ttacaacaat tggatttgga
      361 gttatttgac tatgtcaatt ggtggaacca ccttcggttg cacggtacac ttggctacga
      421 gacaccggtt ggttaccgta accagagatt ggcgcagcga atccttgata atgagctcgg
      481 atgtgctaac gctagcgagg cagtctaact ttaactgtta gctcctgccg aagatcgtca
      541 catccgagga ggctcattgt caaggacaat cggaatagca tacggaaaga agttctg
//
LOCUS       DAFGZE010000272          590 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.283, whole genome shotgun sequence.
ACCESSION   DAFGZE010000272 DAFGZE010000000
VERSION     DAFGZE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.283"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>590
                     /locus_tag="LTU54_003249"
     CDS             <1..>590
                     /locus_tag="LTU54_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HBL8297861.1"
                     /translation="KEKVTLGWTPDVIIGRKERPVSCGMRTLYRLFSKGIFDIDTLPM
                     KGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEFGHLEGDTIVGIHHKSAVITLVE
                     RLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFKSITFDCGKEFSNWKAISNQHDI
                     DIYFADPGTPSQRPLNENSNGILRRNGLPKSIDFRE"
BASE COUNT          194 a          114 c          111 g          171 t
ORIGIN      
        1 ttaaagagaa agtcactctt ggttggacgc ctgacgtcat tatcgggcga aaagaaaggc
       61 ctgtttcatg cggtatgaga acactttatc gtttattttc taaaggaata tttgatattg
      121 acacactacc gatgaaaggt aaaagaaaac ccaatggcca tcaggaaaaa cggggaaaac
      181 aacagtatca gcgctcaatc catgatcgac ctgataatta tcctgatttc aattctgagt
      241 ttggtcacct tgaaggtgat acgatcgttg gcattcatca taaaagtgcc gtcattactt
      301 tagttgaaag attatctaaa gtcattatca cgattaaacc caacggccgt aaggcattag
      361 atattgaaac tgcccttaat caatggtttt ctcgcttccc taaaaacttc tttaaatcta
      421 ttacgtttga ctgtggaaaa gaattttcta actggaaagc cattagtaac caacatgata
      481 ttgatatata ttttgcggac cctggaacac cttctcaacg cccattaaac gaaaattcta
      541 acgggattct gcgtcgtaat ggactgccga aatcaataga ttttagagaa
//
LOCUS       DAFGZE010000273          578 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.284, whole genome shotgun sequence.
ACCESSION   DAFGZE010000273 DAFGZE010000000
VERSION     DAFGZE010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.284"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            126..413
                     /locus_tag="LTU54_003250"
                     /pseudo
     CDS             126..413
                     /locus_tag="LTU54_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..>578
                     /locus_tag="LTU54_003251"
     CDS             427..>578
                     /locus_tag="LTU54_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297862.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWV"
BASE COUNT          200 a           91 c          122 g          165 t
ORIGIN      
        1 cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt tcaggttctg
       61 taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa gtggtaaatc
      121 tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga aaaacagttt
      181 ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg gttattttaa
      241 ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg tcaatagacc
      301 gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt aaatggagaa
      361 gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt taagggagga
      421 aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca aatcattttg
      481 aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt tcctaactgg
      541 agaccaagat ttgattttga taaaaaacag tgggttga
//
LOCUS       DAFGZE010000274          577 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.285, whole genome shotgun sequence.
ACCESSION   DAFGZE010000274 DAFGZE010000000
VERSION     DAFGZE010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.285"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>577
                     /locus_tag="LTU54_003252"
     CDS             <1..>577
                     /locus_tag="LTU54_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HBL8297863.1"
                     /translation="TADDTEFVSSATKLDDIIENPGENIPAGYHKVTFTAGEGTSIES
                     GTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKS
                     DADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVV
                     TYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          233 a           92 c          130 g          122 t
ORIGIN      
        1 ttacagcaga tgatacagaa tttgtatcaa gtgcaacaaa actggatgat atcattgaaa
       61 atccaggaga aaacattcct gcaggctacc acaaagtaac cttcaccgca ggagaaggaa
      121 caagtattga aagtggaaca acagtctttg cagtgaaaga cggcgtaagc ttaccagaag
      181 ataaacttcc agtgttgaaa gcaaaagatg gttatacaga tgcgaaatgg ccagaggaag
      241 caactcaacc aattacagca gatgatacag aatttgtatc aagtgcaaca aaactggatg
      301 ataaatctga cgctgacaaa tataatcctg aaggtcaaaa agtgactaca gaattgaata
      361 aggaaccaga cgcatttgag ggaattaaga acaaagaaga tttaccaaaa gatactaagt
      421 atacttggaa agaaaaagta gatgttagcg cagctggaaa taaaaaaggg actgttgtag
      481 tgacatatcc agatggatca tctgatgaag ttgaagtaga tgttacagtg acagacaatc
      541 gttctgacgc cgataaatat gagccaacag tagaagg
//
LOCUS       DAFGZE010000275          544 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.286, whole genome shotgun sequence.
ACCESSION   DAFGZE010000275 DAFGZE010000000
VERSION     DAFGZE010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.286"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            135..>544
                     /locus_tag="LTU54_003253"
     CDS             135..>544
                     /locus_tag="LTU54_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297864.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARL"
BASE COUNT          173 a          116 c           90 g          165 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcat
//
LOCUS       DAFGZE010000276          514 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.287, whole genome shotgun sequence.
ACCESSION   DAFGZE010000276 DAFGZE010000000
VERSION     DAFGZE010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.287"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..132)
                     /locus_tag="LTU54_003254"
     CDS             complement(<1..132)
                     /locus_tag="LTU54_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBL8297865.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAFGZE010000277          508 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.288, whole genome shotgun sequence.
ACCESSION   DAFGZE010000277 DAFGZE010000000
VERSION     DAFGZE010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.288"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(26..328)
                     /locus_tag="LTU54_003255"
     CDS             complement(26..328)
                     /locus_tag="LTU54_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297866.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
BASE COUNT          176 a           88 c           69 g          175 t
ORIGIN      
        1 ggctaccttt tttaggtagc ctactttaat ctttatacct ttctggatca acgaaagtat
       61 acttcaaata gtcataacgc cgatgatcgc tccgtgcgtc tggcacatca gtcacgatat
      121 caaacaaaaa atacacatct ttcttcattc tggtttttgt tgccggaatt ttaaagtagt
      181 tcttattaga gtaatagaga ttgattaata agctatcttc gattgctaaa aagaaaactt
      241 ctgaatccca caccttataa aaatctttga caaatctatt cgaaggatca aatttaaacc
      301 ataattgtgt ttttccttcc atcaacataa tgttcacctc aaaaagagta tacgaactaa
      361 tgttcttttt gtaaagtagg aatatcattg ataatatgga tggaacatat atattaaaaa
      421 gtgttctttt tgaatgctat tttgaatgcc attttcattc atttaacgaa aatctgcgaa
      481 aataaaaaat gctcaaaccc ttataaaa
//
LOCUS       DAFGZE010000278          504 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.289, whole genome shotgun sequence.
ACCESSION   DAFGZE010000278 DAFGZE010000000
VERSION     DAFGZE010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.289"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
BASE COUNT          226 a           70 c           81 g          127 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actc
//
LOCUS       DAFGZE010000279          497 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.290, whole genome shotgun sequence.
ACCESSION   DAFGZE010000279 DAFGZE010000000
VERSION     DAFGZE010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.290"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
BASE COUNT          206 a           57 c           64 g          170 t
ORIGIN      
        1 attatttttt caaattagat caattaatac ccttataaaa gagcataata aatggttttt
       61 acatcgttat ttattgatat taatctcatc caaaaaaatg aaatcgcata cttttatgag
      121 tgattgaatg tgattttaat acatatacca attaaaagaa aactttttag atttttactt
      181 acaaatttat aaaaaaacaa gataattgat actttgattt tgcgaacatt ttatctttct
      241 gcctaattca gctttaatat catgattata tcagtaacaa atcgcttttt tagctaattt
      301 aagcatcaaa taattattct taatcagaaa aaaataacga ataaatggat tttgggaata
      361 ggtaaaaact tgatcggttt ctggctaaaa ataacatgaa aaaggaaata taaaataaca
      421 tggatcgtgg gatgaataaa gaagttttat acgcaaggcg cgtagtaata taaagaaact
      481 taacacgaaa caaaagt
//
LOCUS       DAFGZE010000280          489 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.291, whole genome shotgun sequence.
ACCESSION   DAFGZE010000280 DAFGZE010000000
VERSION     DAFGZE010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.291"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            44..325
                     /locus_tag="LTU54_003256"
                     /pseudo
     CDS             44..325
                     /locus_tag="LTU54_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
     gene            399..>489
                     /locus_tag="LTU54_003257"
     CDS             399..>489
                     /locus_tag="LTU54_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL8297867.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYRE"
BASE COUNT          184 a           61 c           87 g          157 t
ORIGIN      
        1 cttataattt tttgttaatg aacaagatag gaagaaacat ttaatggaca gttttcagag
       61 attgtcacta gtaaatcaat ttgagttatt ggctaaattt gaggaccaaa acagatctta
      121 ttatgaaaga aaaatcgaaa tattgagaga aggttacgag tatcattatg atgatgaaat
      181 ttggagtgat ctttctgagc catttccgaa agaagattct cgatttgtat tagatgtatt
      241 gaacatgtat cgagatatca attttagtaa atcaaagcta aacttagatg aaaaccaaat
      301 tactaattca tactatactc atttcggttc tgttgcaaag ttttccaaaa aatctatttt
      361 agtgtaaaat tgagaaaaaa gacagagagg acagagtaat gaatcatttt aaaggcaaac
      421 aattcaaaaa agacgtcatt attgtcgctg ttggttacta cctgcgttac aatctaagct
      481 atcgtgaag
//
LOCUS       DAFGZE010000281          463 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.292, whole genome shotgun sequence.
ACCESSION   DAFGZE010000281 DAFGZE010000000
VERSION     DAFGZE010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.292"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
BASE COUNT          151 a           90 c           55 g          167 t
ORIGIN      
        1 gcgaaagcac aaaaaataaa aaaattatta cgttattaaa aaaacagatg aaagatcagg
       61 ttttattaga tctcattctt tcatcacttg aagggattta atgaactact ttttcctctt
      121 tgtcgttttt atatattcgt attccttcaa acagtaggat agcctctttc aatgcttgat
      181 atttgaactg tccgttactt aaaaatttag atgtgccgcc ctcttcctag aacatattct
      241 tttttacttt ttcattaatt gacagaccaa tcgaacgaaa ctgtaaattt cgataggata
      301 atgattggtc aaactcattc attttcaaga catctcgatt catattcaag ataggaatct
      361 ttttatcaac atcactttat tcgtattttc ctctaccata aaagcttcac gctccacaaa
      421 attgttttat acactactat tcagcccatt caacgaccaa tga
//
LOCUS       DAFGZE010000282          462 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.293, whole genome shotgun sequence.
ACCESSION   DAFGZE010000282 DAFGZE010000000
VERSION     DAFGZE010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.293"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..430)
                     /locus_tag="LTU54_003258"
     CDS             complement(<1..430)
                     /locus_tag="LTU54_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBL8297868.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DAFGZE010000283          448 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.294, whole genome shotgun sequence.
ACCESSION   DAFGZE010000283 DAFGZE010000000
VERSION     DAFGZE010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.294"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            1..448
                     /locus_tag="LTU54_003259"
                     /pseudo
     CDS             1..448
                     /locus_tag="LTU54_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          187 a           77 c           82 g          102 t
ORIGIN      
        1 agaaaaagat aatcttacgc ctgaacaaaa agaattgtta gaactacgta aaagaaacca
       61 gcaattagaa atggaaaatg atattttaaa gcaagcagcg ctgatattcg gacgaagaga
      121 caagtaatcg atgcgaataa gcatctttac cctatatcag cgatgtgcag aatattaggt
      181 ctatcacgtc agtcctatta ttatcaatca aaaccaaaga aagacgaatc agaacttgaa
      241 gaagtagtcg ctgaagaatt tatccgcagc cgaaaggcct acggctcaag aaaaataaaa
      301 aaagccttat caaaacgagg cattcagatc agccgacgaa aaattagtag aatcatgaaa
      361 aatagaggat taaaatcgag ctatactgtt gcttatttta aagtacatca ttctacttgc
      421 aatgaagcca aaacgacaaa cgtattga
//
LOCUS       DAFGZE010000284          436 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.296, whole genome shotgun sequence.
ACCESSION   DAFGZE010000284 DAFGZE010000000
VERSION     DAFGZE010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.296"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            24..>436
                     /locus_tag="LTU54_003260"
     CDS             24..>436
                     /locus_tag="LTU54_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297869.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFE"
BASE COUNT          183 a           68 c           68 g          117 t
ORIGIN      
        1 taaaaataaa aaggagaaaa aagatgaagc caaatccttc agaagcagga aaacgaatca
       61 aacaattacg cctttcatgt ggttttacta tggaagaact tggaaggaaa atagataatt
      121 caccacgtgc gaccatatcc aactgggaac gtgggaccaa tttaccaaat ccacaaaagc
      181 tgaaattatt atcaactatc actaattcaa caattgactg gattaaatgg ggaactttag
      241 aagaatatat aacttcatac cttatcgata tagggtatga gctatatatt aaggacttcc
      301 cagaaatacc tcacaaagtc tttaaagaca ttcaagaaag atatagtgat acgttttcgc
      361 tagataaaga ctatgagtta ttgaatccta taataaaaaa tatatttaca aaatattact
      421 caaaagattt tgaaat
//
LOCUS       DAFGZE010000285          411 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.297, whole genome shotgun sequence.
ACCESSION   DAFGZE010000285 DAFGZE010000000
VERSION     DAFGZE010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.297"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>411
                     /locus_tag="LTU54_003261"
     rRNA            <1..>411
                     /locus_tag="LTU54_003261"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          108 a           91 c          131 g           81 t
ORIGIN      
        1 gaaagccccc ggctcaaccg gggagggtca ttggaaactg ggagacttga gtgcagaaga
       61 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccagtgg
      121 cgaaggcggc tctctggtct gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      181 gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgttg gagggtttcc
      241 gcccttcagt gctgcagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      301 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      361 agcaacgcga agaaccttac caggtcttga catcctttga ccactctaga g
//
LOCUS       DAFGZE010000286          403 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.298, whole genome shotgun sequence.
ACCESSION   DAFGZE010000286 DAFGZE010000000
VERSION     DAFGZE010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.298"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            46..>403
                     /locus_tag="LTU54_003262"
     CDS             46..>403
                     /locus_tag="LTU54_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297870.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMAS"
BASE COUNT          163 a           69 c           77 g           94 t
ORIGIN      
        1 cgaatattgg ttaaccaaat tagagaacta aaaaggagaa aaaatatggc tcaaaattac
       61 tatgacgaat ttgtcaaatt acctttggat aaaatggcac aaaaaatgga ggatatgacg
      121 ttcctatacc atgaaacacg tgtacccaaa aaacattaca aggaaaaatt gtcagtcgca
      181 gtggaagaaa tgattgaatc aggtgtcgaa atgaatctca ttgcgacgta ctatcgaaca
      241 ctggaagaac tgaaaaaaca aaatggaaaa tggtttttcc aagccttact atgcttggaa
      301 gtaggtgtca aaccaagcac tatcaaacct tccgaatatc aagccttaga actgacttat
      361 gcaaaattcg cacagattaa gaaagctaaa gcaatggcta gcg
//
LOCUS       DAFGZE010000287          398 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.299, whole genome shotgun sequence.
ACCESSION   DAFGZE010000287 DAFGZE010000000
VERSION     DAFGZE010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.299"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..188)
                     /locus_tag="LTU54_003263"
     CDS             complement(<1..188)
                     /locus_tag="LTU54_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297871.1"
                     /translation="MVHSTIGVVVEKSRDNLVFVTEIQTGRSFVVTDKAAKAYQSSDI
                     LTLNLTTKTFVDATEDYP"
BASE COUNT          112 a           84 c           67 g          135 t
ORIGIN      
        1 aatggataat cttctgtcgc atcgacgaac gtcttcgtag tcagatttaa cgttaaaata
       61 tcgctacttt gataagcttt tgccgctttg tctgtgacga caaaagatct gcctgtttga
      121 atttctgtca caaaaacgag attgtctctg cttttttcaa caacaactcc aatcgttgaa
      181 tgaaccattt ctgcttcctc ccttctcttt cttttgatat aatgtagtcg caaatcgcac
      241 cactcaatga gttggcggaa aacaattcat tgaaaaaaag agtcctggac aatcacgttc
      301 aggactcttt tacttctttt attcacttaa caactaacac tctgtgagtg aaatggactc
      361 ttcaatgtga tgttaggatg atttcatgga cacgattt
//
LOCUS       DAFGZE010000288          387 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.300, whole genome shotgun sequence.
ACCESSION   DAFGZE010000288 DAFGZE010000000
VERSION     DAFGZE010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.300"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            132..344
                     /locus_tag="LTU54_003264"
     CDS             132..344
                     /locus_tag="LTU54_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297872.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
BASE COUNT          127 a           61 c           76 g          123 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagcttt
//
LOCUS       DAFGZE010000289          385 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.301, whole genome shotgun sequence.
ACCESSION   DAFGZE010000289 DAFGZE010000000
VERSION     DAFGZE010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.301"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..300
                     /locus_tag="LTU54_003265"
     CDS             <1..300
                     /locus_tag="LTU54_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297873.1"
                     /translation="NKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT          151 a           54 c           84 g           96 t
ORIGIN      
        1 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
      121 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
      241 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
      361 atgaccgaaa aaaaaatgag tctta
//
LOCUS       DAFGZE010000290          376 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.302, whole genome shotgun sequence.
ACCESSION   DAFGZE010000290 DAFGZE010000000
VERSION     DAFGZE010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.302"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            82..>376
                     /locus_tag="LTU54_003266"
     CDS             82..>376
                     /locus_tag="LTU54_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HBL8297874.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTH"
BASE COUNT          133 a           62 c           76 g          105 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccatt
//
LOCUS       DAFGZE010000291          362 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.303, whole genome shotgun sequence.
ACCESSION   DAFGZE010000291 DAFGZE010000000
VERSION     DAFGZE010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.303"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..>362)
                     /locus_tag="LTU54_003267"
     rRNA            complement(<1..>362)
                     /locus_tag="LTU54_003267"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           72 a          113 c           83 g           94 t
ORIGIN      
        1 aaatccggac aacgcttgcc acctacgtat taccgcggct gctggcacgt agttagccgt
       61 ggctttctgg ttagataccg tcaagggatg aacagttact ctcatccttg ttcttctcta
      121 acaacagagt tttacgatcc gaaaaccttc ttcactcacg cggcgttgct cggtcagact
      181 ttcgtccatt gccgaagatt ccctactgct gcctcccgta ggagtttggg ccgtgtctca
      241 gtcccaatgt ggccgatcac cctctcaggt cggctatgca tcgtggcctt ggtgagccgt
      301 tacctcacca actagctaat gcaccgcggg tccatccatc agcgacaccc gaaagcgcct
      361 tt
//
LOCUS       DAFGZE010000292          349 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.304, whole genome shotgun sequence.
ACCESSION   DAFGZE010000292 DAFGZE010000000
VERSION     DAFGZE010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.304"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            <1..>349
                     /locus_tag="LTU54_003268"
     CDS             <1..>349
                     /locus_tag="LTU54_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBL8297875.1"
                     /translation="DIWLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRK
                     LQSVGLYTKTEHRTVKYLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKK
                     NRRNGTLFGFSVST"
BASE COUNT          118 a           72 c           71 g           88 t
ORIGIN      
        1 agatatctgg ttacgaaaga aacgggatac gcaagcagcc tatgctttct taaaacgact
       61 ccataaacag tttggtgagc cgaaagcaat tgtgaccgat aaagcacctt ctcttggctc
      121 cgcctttaga aagttacaga gtgtgggttt atatactaag acagagcacc gaactgtgaa
      181 gtatcttaac aatttaatag aacaagacca tcgacctatt aaacgacgaa ataaatttta
      241 tcaaagtctc cgtacagcct cttccacgat taagggcatg gagaccattc gaggaatata
      301 taaaaagaac cgaagaaatg gaacgctctt cggcttttcg gtgtctact
//
LOCUS       DAFGZE010000293          337 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.denovo.306, whole genome shotgun sequence.
ACCESSION   DAFGZE010000293 DAFGZE010000000
VERSION     DAFGZE010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.denovo.306"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..155)
                     /locus_tag="LTU54_003269"
     CDS             complement(<1..155)
                     /locus_tag="LTU54_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HBL8297876.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTD"
BASE COUNT          120 a           60 c           30 g          127 t
ORIGIN      
        1 ttatcagtta gtaacttttt ataatcataa aaaaaataag tagttctatc tgagcgatac
       61 tcttttataa tactgaattc ggaattaaaa accacgatag gtaactttaa acaaacatga
      121 agagacttta ataagacttc tttatctgaa aacacttttg tttctctctt tcctttctac
      181 atcattaaat agtattttat acgttatcta aaatactatt cctctgtcaa aataagctaa
      241 cttacttcat tataaaatat ccacttatcg ctttcatgta tattccaccg taaaaagtac
      301 attttatcac cgagaactca tcatttttca tcatgat
//
LOCUS       DAFGZE010000294        26273 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.036, whole genome shotgun sequence.
ACCESSION   DAFGZE010000294 DAFGZE010000000
VERSION     DAFGZE010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.036"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(<1..2247)
                     /gene="uvrA"
                     /locus_tag="LTU54_003270"
     CDS             complement(<1..2247)
                     /gene="uvrA"
                     /locus_tag="LTU54_003270"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBL8297877.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIE"
     gene            complement(2257..4251)
                     /gene="uvrB"
                     /locus_tag="LTU54_003271"
     CDS             complement(2257..4251)
                     /gene="uvrB"
                     /locus_tag="LTU54_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBL8297878.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            4510..6012
                     /locus_tag="LTU54_003272"
     CDS             4510..6012
                     /locus_tag="LTU54_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HBL8297879.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            6014..6751
                     /locus_tag="LTU54_003273"
     CDS             6014..6751
                     /locus_tag="LTU54_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBL8297880.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(6922..8115)
                     /locus_tag="LTU54_003274"
     CDS             complement(6922..8115)
                     /locus_tag="LTU54_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBL8297881.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDRKYLYKYVDAFGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRANVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVLENDTKKKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQQVMKKRPKAEPFVIDGRGNFLFVNHKGKPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(8194..8400)
                     /locus_tag="LTU54_003275"
     CDS             complement(8194..8400)
                     /locus_tag="LTU54_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBL8297882.1"
                     /translation="MSNAQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEQVIDKLDAI"
     gene            complement(8393..9535)
                     /locus_tag="LTU54_003276"
     CDS             complement(8393..9535)
                     /locus_tag="LTU54_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBL8297883.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRYPTTDALEIIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CDGVIRRFDLAINDMCGLLDIPVLSEKYRNGGADCRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRGTKAVQAVEELLLTYNPHG
                     LVFYLITDFVQFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDIADVIESEGVFYE"
     gene            10047..10682
                     /locus_tag="LTU54_003277"
     CDS             10047..10682
                     /locus_tag="LTU54_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBL8297884.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            10836..10913
                     /locus_tag="LTU54_003278"
                     /pseudo
     CDS             10836..10913
                     /locus_tag="LTU54_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            11158..11274
                     /locus_tag="LTU54_003279"
     CDS             11158..11274
                     /locus_tag="LTU54_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBL8297885.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            11290..13209
                     /gene="tet(M)"
                     /locus_tag="LTU54_003280"
     CDS             11290..13209
                     /gene="tet(M)"
                     /locus_tag="LTU54_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBL8297886.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            13328..13495
                     /locus_tag="LTU54_003281"
     CDS             13328..13495
                     /locus_tag="LTU54_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBL8297887.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(13555..13908)
                     /locus_tag="LTU54_003282"
     CDS             complement(13555..13908)
                     /locus_tag="LTU54_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297888.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            14412..14831
                     /locus_tag="LTU54_003283"
     CDS             14412..14831
                     /locus_tag="LTU54_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBL8297889.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            14857..15063
                     /locus_tag="LTU54_003284"
     CDS             14857..15063
                     /locus_tag="LTU54_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297890.1"
                     /translation="MICKASSGDEMAVKEILKFYDGYISKLCLRPFYHSESGKIIMQV
                     DEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(15291..15464)
                     /locus_tag="LTU54_003285"
     CDS             complement(15291..15464)
                     /locus_tag="LTU54_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297891.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            15440..16105
                     /locus_tag="LTU54_003286"
     CDS             15440..16105
                     /locus_tag="LTU54_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBL8297892.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(16187..17353)
                     /gene="dnaJ"
                     /locus_tag="LTU54_003287"
     CDS             complement(16187..17353)
                     /gene="dnaJ"
                     /locus_tag="LTU54_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBL8297893.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(17504..19333)
                     /gene="dnaK"
                     /locus_tag="LTU54_003288"
     CDS             complement(17504..19333)
                     /gene="dnaK"
                     /locus_tag="LTU54_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBL8297894.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(19384..19947)
                     /gene="grpE"
                     /locus_tag="LTU54_003289"
     CDS             complement(19384..19947)
                     /gene="grpE"
                     /locus_tag="LTU54_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBL8297895.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(20041..21084)
                     /gene="hrcA"
                     /locus_tag="LTU54_003290"
     CDS             complement(20041..21084)
                     /gene="hrcA"
                     /locus_tag="LTU54_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HBL8297896.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(21318..22483)
                     /locus_tag="LTU54_003291"
                     /pseudo
     CDS             complement(21318..22483)
                     /locus_tag="LTU54_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
     gene            complement(22499..22726)
                     /locus_tag="LTU54_003292"
     CDS             complement(22499..22726)
                     /locus_tag="LTU54_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBL8297897.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            23210..23800
                     /locus_tag="LTU54_003293"
     CDS             23210..23800
                     /locus_tag="LTU54_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBL8297898.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23990..24703
                     /locus_tag="LTU54_003294"
     CDS             23990..24703
                     /locus_tag="LTU54_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297899.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24818..25828)
                     /locus_tag="LTU54_003295"
     CDS             complement(24818..25828)
                     /locus_tag="LTU54_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBL8297900.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25865..>26273)
                     /locus_tag="LTU54_003296"
     CDS             complement(25865..>26273)
                     /locus_tag="LTU54_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HBL8297901.1"
                     /translation="NEDALVIKKEQGFTGTETKSLFLKDKKDNNYVYLTFTTKQTDFK
                     ALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFGYEKQIPIIIDEELLSNEKLVFAPGR
                     PDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN"
BASE COUNT         7606 a         5304 c         4781 g         8582 t
ORIGIN      
        1 ttcgatctta ataattccat ccccgcgaca agcttcacaa cgaccgcctt ttacgttgaa
       61 actaaaacgc cctttcttat aaccccgcat ttttgcctcg ttcgtttgag caaataaccc
      121 acggatatca tcaaatacac ttgtataagt cgctggatta cttctcggcg tccggccgat
      181 tgggctttga tcgatatcga tgatcttttc gatactttcg tagccggaaa ttgtcttgaa
      241 tttacctggc ttagcagaat tcttatttaa tttttgcgct aacgatttct ttaagatact
      301 attgaccaat gtactctttc cagaaccaga cacacctgtg actgctacaa attctcctaa
      361 tggaaattca acactaatat ttttcagatt gttttctgaa gcaccagtga tcttgattgc
      421 tttcccatta cctttacggc gttcttttgg tacagggatt gattttttcc ctgaaagata
      481 tttccctgtc aaagaatttt catcattagc tacttcttca ggagtgcctg cagccacgat
      541 ttgtccgcct tgttctccgg ccccaggtcc gacgtcaatc aaataatctg ctgcccacat
      601 cgtatcttca tcatgttcga ctacaacgag ggtgttgcca agatcacgca ttttctttaa
      661 tgagccaatc agtcgatcgt tgtcgcgttg atgcaatccg atcgatggtt catctaaaat
      721 atacaaaact ccagaaagat tcgatccaat ttgtgtagcc aatctgattc gctgcgcttc
      781 tcccccagat aatgttccgg aagcgcgact caaagttaga tagtctagtc ccacattttc
      841 caagaatgaa agtctgtcat ttacttcttt taaaatcggt ttggcaatta ctttctcttg
      901 ttcagataac gttaagtttg agaagaaatc tactgctttt tgaatagaca actcatttgt
      961 ttgcccgata ttcgttccat tgatttgaac agatagagct tggggattca atcgataacc
     1021 tttacatgtc tgacaagtta attctgtcat ataaagacgc atttgctcac gcgtgaaatc
     1081 actgttcgta tcatgataac gacgctcgat attcttcaat accccttcga aagtcgtttc
     1141 tacatcgcga acgccaccaa aatcattttc ataatggaaa tggaaaagat catctgtccc
     1201 attcaagatg atctcctgat gctcttccgg taattcttcg aatggtgtat ctaaatcgat
     1261 accgaaactt tcacaagcct gcgctaacat ttgaggataa tactgagagc tgattggatt
     1321 ccatggaacg atagcacctt gactaagtgt cttcgttcta tctggaatca ctaaatcttg
     1381 atcgacttct aatttgacac ctaaaccatc acagtctgga caagctccaa aaggcgcatt
     1441 gaatgagaac agtcttggtt ctagttcgcc aacagtaaat ccgcaataag gacaggcata
     1501 gtgttcgcta aacaacattt cttcttcacc gatcacgtca accagagcat agccgtctgc
     1561 taatcgcagg gccgcctcta aagaatcaaa taggcgagaa cgaatccctt ctttcacaat
     1621 gatccggtcg atgacgatcg cgatatcatg ttttttgttt ttctccagct caggcacttc
     1681 acttaaatca tagatctcac catcgactcg aacgcggacg tatccctctt tttggattcg
     1741 ttcgaatatt tttttatgtt gtcctttttt ctttaccacg atcggtgcca acacttggat
     1801 ctttgtgcgc tcaggcaaag ccattacttg atctgccatt tgatctactg attgactggt
     1861 aatctcgata tgatcgttgg gacaaatcgg atgtcctaca cgtgcaaaca acaagcgtaa
     1921 atagtcattg atttccgtca cagtacctac tgtcgaccga ggatttttac ttgttgtttt
     1981 ctgatcaata gaaatcgccg gactcaatcc gtcgatgcta tctacgtccg gcttatccat
     2041 ttgtcctaaa aactgacgag cataagcaga aaggctctct acgtatctcc gctgcccttc
     2101 agcgtacagc gtatcaaatg ctagggaact ttttccagaa ccggacaaac ctgtaacgac
     2161 cacgaattta tctcgtggaa tcgttacatc tatatttttc aagttatggg cacgtgcacc
     2221 atgaatcgta attttatcgt tagccatgtt gtcctcctac tttgaagctt ttaattctaa
     2281 aatcatatct cgcacattcg cggctgtttc aaaatccaaa gcttttgccg catctttcat
     2341 ttctttttct aatttcatca ataacgtgtc tttttcttcc ttcgtcattt cttcgtacga
     2401 gacttgtacc gcttcttttg tatcatcttc cgattctttc gtaatagaaa tcaaatctcg
     2461 gatttcttta atgatcgttt ttggaacgat cccatgttct tcattgtact tctgctgaat
     2521 cgtacgacgt cttgacgttt catccatcgc tagacgcata gaatctgtta ctttgtctgc
     2581 gtacatgatt actttacctt cttcattccg agcagctcgt ccaatcgttt ggactaagga
     2641 acgttcacta cgtaaaaagc cttctttatc cgcatccaag atagcaacaa gggaaacttc
     2701 aggtacgtct aatccttcac gaagcaaatt gattccaact aacacatcaa attcacctaa
     2761 tcgaagatct cgaatgattt ctgttcgttc cagcgttttg atatcactat gcaaatattt
     2821 gactttgatc cctaattctt tgaaataatc tgtgaggtct tctgccattt ttttcgtcaa
     2881 tgtcgttaca aacacccgct ggtctttttc gactcgttca tttatttcgc ctactagatc
     2941 atcgatttgt cccatgattg gacgaacttc gatcactggg tcaagcagcc ctgtcggacg
     3001 gatgatttgt tggataacgg tatctgtttg ttcatgttca tatggtcctg gcgtcgcaga
     3061 gacataaatg atctgattga catgtttttc aaactcttct aaccgaagtg gccgattgtc
     3121 taaagcagat ggcaaacgga aaccgtaatc tactagcatc tgttttctcg ctcggtcacc
     3181 attatacatt cctctgattt gaggcatggt tacgtgcgat tcgtccgcca cgatcaaaaa
     3241 atcttcagga aagaaatcca atagcgtata aggcggttca ccttctttac gtccgtccat
     3301 atgccgagaa taattttcaa ttcccgacgt gtagcccatt tctaacatca tttcaatatc
     3361 gtaattggtt cgctgttcta aacgttgagc ttccagaagt ttgttttcat ttcgcaagac
     3421 agttaaacgc tgttctaatt cctctttgat attcgctacg gcatgctcca tatgttcatc
     3481 attcgtcaca aagtgagtcg ccgggaagat tgctacgtgt tccgtttctc ctaaaacttc
     3541 acctgtcagt gcattgactt ctcggatacg ttcgacctca tctccaaaaa actccacacg
     3601 taaggcacgt tcatctcttg aagccggaaa aatctccacg acatcaccgc gcactcgaaa
     3661 acgaccgcgc tgaaaatcta tatcatttct ttcgaattgg atactgacta agtcacgaat
     3721 caactgattg cgatctagtt ctgctccctg tcgaatcgaa actacttgtt tttggtactc
     3781 aaaaggcgag cctaatccaa aaatacatga aacggatgca atgacgatca catcatttct
     3841 ttctaataat gaactagtag ccgaatgtcg taatttgtcg atctcatcat ttacacttga
     3901 atctttttcg atatacgtat cgcttgaagg cacataagct tcaggctgat aataatcata
     3961 ataagaaaca aaatattcaa cagcattgtt agggaaaaat tccttgaatt ccccgtacag
     4021 ctgaccagcc aatgttttgt tatgggcaat aatcagtgtt ggtttattca ctttctcaat
     4081 aagattggaa atcgtatatg ttttccctgt tccagtcgca cctaataaga tttgtgcttt
     4141 tttcccgccg actacgccat ccactaattg gttaatcgct tcaggctgat caccagccgg
     4201 ttggtatttc gagacaagct caaagtgtcg ggaggtatct ctctcaatca ttcttcgtcc
     4261 tccttttcat tcattccttt tcattttacg accaataaaa agaaaaggaa agcaaaggca
     4321 ttccttacta aaaaattata ccacaccgaa catactttcg ccactttttt atcttattat
     4381 acatgttatg ttttataaca taaaatgtta gaaactcgca tttttttcat tttttcgagc
     4441 ttacagtctt gtctgattca gcacttttcg cctataataa taaaatattg gaaatttagg
     4501 aggttgatta tgaaaagaaa gcatttattt cactcatttg ttttcttaat gacaggactt
     4561 gtgacacttg cgtttggtca tgttgcacaa gctgacaaca ctacaacggc agagtcaagc
     4621 acaagcgaga caatgcaaaa aatagagccc aaaaaagacg tatacgtaat tgccagtgac
     4681 tctgcttttg ctccatttga atttcaaaac gcaaagggcg agtacgaagg catcgatgta
     4741 aaattgatgg aaaacatcgc caaactgcaa ggatttaaca tccgaatgga ctaccgtggt
     4801 ttctctgctg ctttgcaagc tttggaatca ggacaagcag acggaatgat tgccggaatg
     4861 actgtaacag atgaacgaaa gaaaagctat gatttttctg attcttactt tgatagcggg
     4921 atccaattag ctgtcaaaaa aggagatact tcgatcaagt cttatgaaga tttgaaagga
     4981 aaaacagtcg gagcaaaagt tggtaccgaa agtgctgaat ttttaagcga ccatgaatct
     5041 gaatatggct attctatcaa atattttgat gctgctgatc aattgtacga cgggttaaaa
     5101 gttggcgcaa tcgatgcaat gatggatgat tatccggtaa tcggttatgc gatcaaacaa
     5161 ggacaagaat tggaaacacc gatcaaaaaa gaatctggcg gaaaatatgc atttgccgtt
     5221 aaaaaaggac aaaatcctga gcttcttaat atgttcaatg aaggattgaa agaattaaaa
     5281 agaacaggcg aatacgataa gattattaaa gaatatgtag cggatggttc agaaacttcc
     5341 agtgagtctt ccactgctga tgaatcaaca tttcttggac ttatccaaaa caactatcaa
     5401 gcattgttga aaggactttg gagaacaatt ctcctaactt tgatctcctt tgctttggct
     5461 atagtaatcg gtgtgatttt tggattattc agtgtagcac caatacgcgc tcttcgcaca
     5521 attgctagta tttatgtcga tatcatccga ggaattccaa tgatggttct tgcgttcttt
     5581 attttctttg ggttgcctgg tatcgttgga tttacgattc ctgacttcct tgctggtgtt
     5641 atcaccttga ctcttaacgc aagtgcttat atcgcagaga ttgttcgagg cgggatcaac
     5701 gccgttcctg tcggacaaat ggaagcttcc agaagtttag gtctttctta taatcggaca
     5761 atgcaaaaaa tcattttgcc gcaagcgata cgcatcatga tcccatcatt cgtcaatcag
     5821 ttcgttatct cattaaaaga cacaacgatc atttccgcaa tcggtgtagt cgaattgctg
     5881 caaactggta agatcattgt tgcacgtaca acacaaagta cgtatgttta cttgatcatt
     5941 gcgatcatgt acttgatttt gatcacagca ttgacaaaat tagcaaaagt tttagaaaag
     6001 aaggtgaagt aagatggcag aaaaaatctt agttgagcat ttagtcaaaa aatacggcga
     6061 taacacagta ttaaacgaca tcaatgtatc catcaacgaa ggagatgtcg tctgtatcat
     6121 tggcccttca ggttcaggaa aaagtacatt cttgcgttgc ttgaatcgtt tagaagaacc
     6181 ttctgacggt gaaattatta ttgacggcgc ccacttgatg gataaaagca cagatatcaa
     6241 cttggtgaga caacatatcg gaatggtatt ccaacacttt aacctattcc ctcatctttc
     6301 tattttggaa aacatcattt tagcaccgat ggacctgaag aaagaatcta gagaagaagc
     6361 agagaaaaaa gcaatcaaat tactgcagac agttggtcta gaagataaaa aagatatgta
     6421 tcctgaaatg ctttctggtg gacaaaaaca gcgtgtggct attgctcgtg ctctagcgat
     6481 gaaccctgac atcatgcttt ttgatgagcc tacttctgca ctcgatcctg aaatggtcgg
     6541 tgatgtcttg aacgtcatga aaaagttagc agaacaaggt atgacgatgg ttatcgttac
     6601 gcacgaaatg gggttcgcta aagaagtggc caatcgcgtc atgttcattg acgacggaaa
     6661 ctttttagaa gatggaacgc ctcaacaaat ctttgaaaat ccgcaaaatg aacggacaaa
     6721 agatttcttg aataaagtat taaatattta aaataaatta tatatgtctt tttgactatc
     6781 tttttatatc ttcttaaatg tccgcaaagc cttattttat aggctctacg ggcattttgc
     6841 ttttgtggta aacctcacat atctaggtct atcttcttat attttcacta tcaagcgtgg
     6901 ttaaaatcgt ggtaaatact tctatgcaat tagacgctga acctctgatt ttgcggtatc
     6961 tatggacgca tgagcatacc agttcattgt gatactgata tttgaatgcc ccatgatata
     7021 ctgtaaatct tttgggttca tgttcttgct tgccagtctt gtgcagaatg tatggcgtag
     7081 cgtatgcggt gtgatatgtg gcaatggatt gtccttgtgg tgcttgttgt atttctttac
     7141 catacggata aataaggcgt tataatcaat cgcaactttg ggcttgccct tatgattgac
     7201 aaataggaag ttgccccgtc cgtctatcac aaatggttct gcctttggac gttttttcat
     7261 aacctgttga aatgcctgta tcgtttcttt gcttaatggt acttgcctta ttccgctttt
     7321 tgttttaggc gtttcaatat aatagccctg ttccttgctc tttagtaact ggtggtcgat
     7381 aatcacaact tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgaaat
     7441 acgaagtcct gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacattgtc
     7501 cgtcttgatg aatgagagta aggcttgttc ctgttcctct gtcaatgcga ctttcttttt
     7561 ggtatcattt tctaggactt cactcaactt gaaatcaaag ggatttttcc tcacataatc
     7621 atcttgaatg gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt
     7681 gtaggaaaag cctttgtctt tcatgcgtaa cgcccattcc ttagcgtcag agggtttgat
     7741 tgtatcaatg ctcctagcac ctaacttgtc ctctttcaat aaccgcatga gttgttcccg
     7801 ttgtttctgt gtgcttttct tcacatttgc cctctgtgcg ttctgtttgg cataaagttg
     7861 gcaaagcgtc attttcttgc cagtgctgtc gataccgtcc tcaatatccc gtcttatctg
     7921 ctgttccagt tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg
     7981 tgtcaatctc caagcataca catattttgt gtttccaaat gcgtctacat atttatataa
     8041 gtattttctg tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg
     8101 tcttttttct ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg
     8161 tgttcatcat aacacataca aggctcattt gcattagata gcgtccaatt tatcaatgac
     8221 ctgttcaaac tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc
     8281 gtccttgttt tcctctgcca atcgtcttaa cttgttttct ccgatacgga aatactttga
     8341 cgcttcttca atggtaaggg tgtatttttc ccatacggga atatcctgtg cattactcat
     8401 aaaacacccc ctcactttcg attacgtctg ctatgtccgt acacatctgt tccacaagca
     8461 tttcctgctt ttcagagagg tgggtacatt catcaatcat tttcatgtag tttgagcctg
     8521 tcagcctgtc gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt
     8581 gcagagtgcg ttccatattg acaggtacgg ggttcatttc aaaaggtaaa ctgtcatggg
     8641 aaatgaatat ctcccacagc ggatagtcgg gaaattgcac aaaatcagta ataaggtaaa
     8701 acaccagccc atgagggtta tatgtcaata acaattcctc aactgcctgt actgcttttg
     8761 taccacgcag acgaatttca aaacgattga taatatccgt atgtttcttt ttggttgcct
     8821 gttccttttg tttttcatac aggcagaagt attttgtgct tgcctttgaa ccgatataaa
     8881 gggtgcttgc taaattacgg ttttttccgc ttagttttcc accctgcaca ttttcataat
     8941 ttttacagcg acaatccgca ccgccatttc tgtatttttc agacaaaacg ggaatatcca
     9001 acagaccaca catatcattg attgccaagt caaaacgtcg gataacaccg tcacagtcaa
     9061 gacagcggtt taaaaatgaa taccaatctc tgttctgtgc ctgcaagaca tattccatgt
     9121 tgcgtgagcc tgcccctttt aattccaaga atacgcccat gtgttcgtca tcagaagcca
     9181 tgaccttgat ttctccgtag gcgtaatgct ctttatagcc ataataccca tagtcataat
     9241 ggataaaata atctgctttc atgccaagca cctttcggat aatttccaat gcgtcagtcg
     9301 ttggataacg gacagacaga taatcaacga gcagataaaa aggcttatcg cagatataat
     9361 aattcagaca acgcaggata atattttgtg tttccttgct ggcgttggct tttccgttct
     9421 caaagcggtt gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa
     9481 gaccgcttgc aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat
     9541 tcctccaatc ccacaatagc cttgaaatac ctgtaaacct ctattcaaaa agtgtgatat
     9601 gcactatctt cttaaaactt ctcattacta ctcacaactt tgggattgta tggtttttga
     9661 cttgtctttt gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca
     9721 ggctcgccag ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct
     9781 gagaggcttc cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct
     9841 aacaagttcc atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt
     9901 ttgaatgttt ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct
     9961 tatatgctta tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac
    10021 ttataatagc gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag
    10081 gaaataatat acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca
    10141 aaatcggtat caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct
    10201 atgaaacgct ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg
    10261 gagaatggaa gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag
    10321 aacgtctaaa tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag
    10381 aatggctgga atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga
    10441 aaagcattga agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc
    10501 aaaaattatt agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag
    10561 agggacataa gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag
    10621 aagatatgga actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt
    10681 aacttttttc ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc
    10741 accaccaatg gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag
    10801 aggaagaaag ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatctc
    10861 aatttgattt ggtacttgaa aagaacggga gtaattggaa gattatagaa taacaaatat
    10921 tggtacatta ttacagctat tttgtaatca cgtactctct ttgataaaaa attggagatt
    10981 cctttacaaa tatgctctta cgtgctatta tttaagtatc tatttaaaag gagttaataa
    11041 atatgcggca aggtattctt aaataaactg tcaatttgat agtgggaaca aataattgga
    11101 tgtccttttt taggagggct tagttttttg tacccagttt aagaatacct ttatcatgtg
    11161 attctaaagt atccggagaa tatctgtatg ctttgtatgc ctatggttat gcataaaaat
    11221 cccagtgata aaagtattta tcactgggat ttttatgccc ttttgggttt ttgaatggag
    11281 gaaaatcaca tgaaaattat taatattgga gttttagctc atgttgatgc aggaaaaact
    11341 accttaacag aaagcttatt atataacagt ggagcgatta cagaattagg aagcgtggac
    11401 aaaggtacaa cgaggacgga taatacgctt ttagaacgtc agagaggaat tacaattcag
    11461 acaggaataa cctcttttca gtgggaaaat acgaaggtga acatcataga cacgccagga
    11521 catatggatt tcttagcaga agtatatcgt tcattatcag ttttagatgg ggcaattcta
    11581 ctgatttctg caaaagatgg cgtacaagca caaactcgta tattatttca tgcacttagg
    11641 aaaatgggga ttcccacaat cttttttatc aataagattg accaaaatgg aattgattta
    11701 tcaacggttt atcaggatat taaagagaaa ctttctgccg aaattgtaat caaacagaag
    11761 gtagaactgt atcctaatat gtgtgtgacg aactttaccg aatctgaaca atgggatacg
    11821 gtaatagagg gaaacgatga ccttttagag aaatatatgt ccggtaaatc attagaagca
    11881 ttggaactcg aacaagagga aagcataaga tttcagaatt gttctctgtt ccctctttat
    11941 catggaagtg caaaaagtaa tatagggatt gataacctta tagaagttat tactaataaa
    12001 ttttattcat caacacatcg aggtccgtct gaactttgcg gaaatgtttt caaaattgaa
    12061 tatacaaaaa aaagacaacg tcttgcatat atacgccttt atagtggagt actacattta
    12121 cgagattcgg ttagagtatc agaaaaagaa aaaataaaag ttacagaaat gtatacttca
    12181 ataaatggtg aattatgtaa gattgataga gcttattctg gagaaattgt tattttgcaa
    12241 aatgagtttt tgaagttaaa tagtgttctt ggagatacaa aactattgcc acagagaaaa
    12301 aagattgaaa atccgcaccc tctactacaa acaactgttg aaccgagtaa acctgaacag
    12361 agagaaatgt tgcttgatgc ccttttggaa atctcagata gtgatccgct tctacgatat
    12421 tacgtggatt ctacgacaca tgaaattata ctttctttct tagggaaagt acaaatggaa
    12481 gtgattagtg cactgttgca agaaaagtat catgtggaga tagaactaaa agagcctaca
    12541 gtcatttata tggagagacc gttaaaaaat gcagaatata ccattcacat cgaagtgccg
    12601 ccaaatcctt tctgggcttc cattggttta tctgtatcac cgcttccgtt gggaagtgga
    12661 atgcagtatg agagctcggt ttctcttgga tacttaaatc aatcgtttca aaatgcagtt
    12721 atggagggga tacgctatgg ctgtgaacaa ggattgtatg gttggaatgt gacggactgt
    12781 aaaatctgtt ttaagtatgg cttatactat agccctgtta gtaccccagc agattttcgg
    12841 atgcttgctc ctattgtatt ggaacaagtc ttaaaaaaag ctggaacaga attgttagag
    12901 ccatatctta gttttaaaat ttatgcgcca caggaatatc tttcacgagc atacaacgat
    12961 gctcctaaat attgtgcgaa catcgtagac actcaattga aaaataatga ggtcattctt
    13021 agtggagaaa tccctgctcg gtgtattcaa gaatatcgta gtgatttaac tttctttaca
    13081 aatggacgta gtgtttgttt aacagagtta aaagggtacc atgttactac cggtgaacct
    13141 gtttgccagc cccgtcgtcc aaatagtcgg atagataaag tacgatatat gttcaataaa
    13201 ataacttagt gtattttatg ttgttatata aatatggttt cttgttaaat aagatgaaat
    13261 attttttaat aaagatttga attaaagtgt aaaggaggag atagttatta taaactacaa
    13321 gtggatattg tgtcctgtat gtggaaataa aacacgatta aagataaggg aagatactga
    13381 attaaaaaaa ttccccctct attgtccgaa atgcagacaa gaaaatttaa ttgaaataaa
    13441 gcagttcaaa gtaactgtga ttacagagcc agacgcaaag acgcagagcc gataaaatga
    13501 gattaataca atctcatttt atcggctctt tccgttatgt atggattctt ttaattagtc
    13561 ttcgatgttt cttgcttcgt tgataccgct ggctaaagat tccattaagg atagttcttt
    13621 gtctgtaaag ctatccatgt atttctctat ctgtaatcgt cgggtgcttt ttaccaagtt
    13681 attagcaggt aagaaaaatt catcaacgga aacatgaagt aacgatacaa ggtcataaag
    13741 aacttgtatg ctggggtgtt gccctttatt ttcaatatta gttaagtacc gtgggtcaat
    13801 ttcaatcaat gctcccactt gttcacgagt taaacctcgt ttcaatcgag cttctttaat
    13861 ggctaaacca aaggctctaa aatcatattt atcttctttt ttacgcatag tagaccacct
    13921 ctatacattt tattgttcct actgaattaa aaacaggtat agaaaaacgt gttatatggt
    13981 ttataggttt atatttaata aaaagcacta ctaaacgcca ataaaaaaaa ccgttatatg
    14041 gtagtgctat ttacgctgtt aaaatattgt atattacttc caaatggcgg tttgttggag
    14101 gtcaacgtcg ccatgaagta catcatatac aataaatttc cttacattgg gttcttgtca
    14161 aaaaaagtcg tctatctgca atagataagt acgtccacca atgtggtttt ataaatcata
    14221 tagatagaat aacagaagca tgtaaacaga gaaataaatc tgtttatatg cttttttggc
    14281 tattcagaac ttttttacaa agtttattta tcagtaatgc aacaaatccc cctttcacat
    14341 tgggactaag agtgaaagga gataaacgag caaggctcac ttcctttcct agacagaaag
    14401 ggggtgagat tatgaggaca tcttctttcg agcatatcgt cagattacag ttcaattctt
    14461 taatgctgat tgtcattaag aacaaagtga aaagtcgaaa cagacagctt gcaagacatt
    14521 ctaaacgtga agtattgttt tgtgaaatag ccgaaacgaa acaagttgaa cgtggaacga
    14581 ttgatacctg tttttatgat tgtgtatcat ttaaagtctt gcactttaca atttatgtat
    14641 ctgatgaaac acttggtaca gttttacata gactatcaga aaaacagcgt agtgcgattt
    14701 tattacgtta ttttcaagga atgaacgaca gagaaatttc agaactgtac catgtatcac
    14761 gttctgctat ttgcagtaga agaactagag gactaaggaa gttaaaaatg cttttgagcg
    14821 aaaggaagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
    14881 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
    14941 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
    15001 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
    15061 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgttatctcc cttagtagtt
    15121 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
    15181 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
    15241 atttaataaa aaccaaaaat gcacggtcgc catgaaggga ccgtgtattt ttatcttctt
    15301 attttctcac ctaatcgtat cagccgcatc gcttgttttc ctgcacaaac aagcaatcgc
    15361 tctacctgtt ccatcgcata agaaagtacc atcggttcat tgatgatatc aagtacatag
    15421 tcgataccac cctcacttaa ttgagcttga tttttcttct ccatattatg tagttgtggg
    15481 acaactttct gacaatcagc aattgtgtca agaacggatc aatcctttat tggagttgct
    15541 cgaaagggtc ttctcttatt acggacaggc tttgctcact gtacatagac aacaaatcat
    15601 tatcctagtc aatcggattt ccagagcctc attgctatca ttgcttgata aaatccagac
    15661 aaagtttaat aaaatgtatc agttacagct aaactttgga attgggtcgc tttgttatac
    15721 agagcaagag acaccgcaat ctttccttca tgccaaacaa gtttgcgaat ggatagcttt
    15781 ccatcaatct gtgaacgaga tccgcttttt tgaagattta gacctcggga tcgttttacc
    15841 agcaatcccg tcagatcaac gaacattata tgtaaaacgt attttgaaaa gcctgacaga
    15901 agaagaagta catttgttca agaagacttt ggcatgcttc agtaaaaata acggaagcat
    15961 caaaaattgc tcagaagaat tatttattca taaaaacacc ttgcaatatc gattgaacaa
    16021 gttccattct ctcactggtt atacccctag aaactatgac gattatcata ttctaaagct
    16081 ggcttttttg ttggtacaaa catgacaact acaaaaaaac caggcgaaca tatgattgtt
    16141 cgtctggttt ttaattatta atgacttagc gtttttacta gaaaaactac ttccgctttt
    16201 tcccgccaaa agcatcttta aacttatcga agaaaccgtc ttcttgctgt tcttcaactt
    16261 tgatgccgcc agcttcagcg aatgcacgta atgcttctct ttgttgttca tttagatttt
    16321 tcggtgtgat cagttttact ttgacatgtt ggtcgcctgt tgcaccacca cgtaacttcg
    16381 gagcgccttt tccacgtaaa cggaagttcg tacctgtctg tgttccagca gggatcttca
    16441 atttcacatc tccatggaca gttggtactt ggacttcatc ccccaaagct gcttgtacaa
    16501 aacttaaagg caattcatag taaatttcag aaccttcacg atcaaagata tcactttctt
    16561 ctacacggaa gaccacatac aaatcaccaa atggtccgcc gttttctcca gcttctcctt
    16621 gaccagctaa acgcatttgt tgtccttctt ctacaccagc tggtacattc actttaactg
    16681 aatgtgcttt cttctcatgt cctgagccat gacaggttgg acaaggttct ttgatttctt
    16741 tccctgtccc atgacaaaca tcacaagttt gacggctcat cactcgtcca agtggtgttt
    16801 ggcgttctac attaatcgta cctgcaccat gacatttatg acaagtagtt ggttgtgtgc
    16861 ctggttttgc accgttccca ccacaagtat gacagatttc ttcccgatta tacttgattt
    16921 ctttttctac accaaaaatc gcttcttcaa aagacaagtc gaccgtatat tgcaaatcag
    16981 caccttgacg aggcgcattt ggatcgactg accgaccgcc tcctccaaag aaagagtcga
    17041 agatatcttc aaaaccaccg aagccgccac cagagaagcc gccgaatcca ccaaatccgc
    17101 caccagctcc accagcaccg taatttggat ctgtgcctgc atgcccgtat tggtcatatg
    17161 cagcgcgttt ttgcgggtca cttaaaatct catatgcttc tgatacttct ttgaattttt
    17221 cttccgcatc aggttcttta ttgatgtcag gatggtattt tttcgaaagc ttacgataag
    17281 ctttctttat ttcatcttct gatgctcctt ttgagagccc taagacttca taataatcac
    17341 gttttgttgc cataggcttt cctcctattg gttctcatca ttttcacgtc gcttgttatc
    17401 atatcatatt ttgtttgaaa caaaaaccga tacttcaaac tatttttcaa aagaaaaagc
    17461 caaagtagca actttggcct tctctcgaaa ttcagtgaac tgattattta tcgtcgccat
    17521 cgacttcttc aaaatcagca tcaacgacat catctgctcc gccttgagct gcttctgggt
    17581 tttcttgagc ttgttgttgt gccgcttgtt cgtatagttt aacagtcaag ttttgaacga
    17641 tttcgttcaa tgcatcgcgt ttagttttca tttcttcgat gttgtttgct tcaacagcgg
    17701 ctttcaattc atcgcgagct gtttctgctt ttttcacttc ttcttcgtcc actttgcctt
    17761 ctaattcttt caatgttttg tccacagaga atagcaatgc atctacatca ttacgtaggt
    17821 caacttcctc tttacgagct ttatctgctt ctgcatttgc ttctgcatct tttaccatac
    17881 gttcgatttc ttcgtctgtt agacctgaag aagatttgat cgtgattttt tgttcttttt
    17941 gtgtacctag atctttcgca gatacattta caatcccgtt tttatcaata tcgaatgtta
    18001 cttcgatttg tggaatacca cgaggagctg ctggaatatc tgtcaattgg aatcttccta
    18061 gagttttgtt gtcagcagcc attgggcgtt caccttgtaa tacatggatg tctactgctg
    18121 gttgattatc tgccgcagtt gagaatactt gtgatttact tgttggaatc gttgtattgc
    18181 ggtcaatcaa tttagtgaac actccaccca ttgtttcgat acctaatgac aatggtgtaa
    18241 cgtcaagtaa aacaacatct ttcacatcac cagtgatcac gccaccttgg atagcagcac
    18301 ccatagcaac tacttcatca gggttaactg atttgtttgg ttcttttcct gtttctttac
    18361 gtactgcttc tacaactgca gggatacgag tagatccacc gactaaaata acttcgtcga
    18421 tttctgattg tgataaacct gcatctttta gcgcttgacg tactggtact tttgtacgtt
    18481 ctactaagtc agctgttaat tcatcaaatt ttgcacgtgt caatgtcatt tccaaatgca
    18541 atggtccaga ttctcctgct gtgataaatg gcaagctgat ttgtgtgctt gatacgccag
    18601 ataaatcttt tttcgctttt tcagcagcgt ctttcaagcg ttgtaaagcc attttgtctt
    18661 gagacaagtc aatgctgttt tcttttttga attctgctac catgtagtcg atgattttgt
    18721 tatcaaagtc gtccccacct agatggttgt cgccagctgt tgacaataca tcaaagacac
    18781 cgtctcctag ttcaaggata gatacgtcaa aagtaccgcc accaaggtcg aatactaaga
    18841 ttttttcgtc gcgatctgtt ttatctaaac cataggcaag agcagctgca gttggttcat
    18901 tgacgatacg ttctacttct agaccagcga tcttaccagc atcttttgtt gcttgacgtt
    18961 gtgcatcatt aaagtaagct ggcacagtga taactgcttt ttcgactttt tcacctaaat
    19021 agtcttctgc gaatcctttg atatattgaa ggatcattgc tgagatttct tgtggtgtat
    19081 atgatttacc ttctacatct actttgtaac ctgcttcacc gatatggcgt ttgattgatg
    19141 aaatcgtatt tgggtttgtc acagcttgac gttttgcaac ttcccctact tggatttctc
    19201 catttttgaa tgatacaaca gaaggtgttg tacggtttcc ttctgggtta gcgatgattt
    19261 ttgcttcgtt tccttccaat actgctactg cagagttagt cgtacctaag tcaataccga
    19321 taattttact cataattaac gtctccttct ttaattgaaa gtgtaagttt tatatccgca
    19381 caattattgt gcaacaatga ccatggttgg tcttaacacg cgatcatgta atttgtagcc
    19441 ttcttgtagt acttctacaa tcgtatcggc tggtgtatct tcagtcgctg gaacagtttg
    19501 tactgcttga tgaagattag gatcaaaggc ttctccttta gcagggattt tttcaattcc
    19561 ttcttcttcc agtgcgttac gcaaactttc caagaccatt tccacgcctt ttttcaaacc
    19621 tgccccttga tcatctgaca cttctgtggc taatgcacgt tccaggttgt caattgatgg
    19681 aagtaatttt ttggctaaat cttgtgaacg gtatttttgt aattgctcac gctcattttt
    19741 tcctcgattt gccatatttg cgatttcagc acgagcccgc aagtatttat cttccatttc
    19801 ttctaactca gcttttaatt tcgctgtttc gaattcttcc gcttcagctt ctgaaatacc
    19861 cgaatcttct gcagcttcga catcgatttc cggttctggc tgagcatctg tcatttcttt
    19921 atctagttct tcttgatttt ctttcacgat acagtcttcc tttcattatt cagtcagctg
    19981 tttgattgct cttactaaga aagagagaac gatcagctgc aaatagcttc gttattcaca
    20041 ttacgagttt gacaagtcaa gcgaacggta gtaatcagca agtttagctg ctagttctct
    20101 tcgataaaca tcgactagac cgaacatccg agaataaggc atactagtag gccctaacaa
    20161 ggctattgta cctcttccat gaccatctat ctcgtaactt gcttgaatca tactcatatt
    20221 ctgcaataaa tcattgccta attcagcacc aatcttgata tgaatattgc tatccatcgg
    20281 agtgatcatt tgtgtaagtc cgtctgagtc tttcataaaa gaaaacatcg atttgaactg
    20341 atcaagatct tgacggggct cataatccaa gagattcatt tgtccactga cataaacctt
    20401 atcttcaaaa acatgaccaa gaacggtgtc aaataactga gacatccctt ctgtcgtttg
    20461 gaaatattta tgaagaatca tcggaatctc cgtacgaagt ttatggtaca ccgtcatcag
    20521 aggatctccg actagacggt cgtttacaat ctgtaccatt ttttctagtt cctgacttcc
    20581 catatattcg ggaagagtga acacttggct ttctacattc cccatatctg ttacgacgat
    20641 agcaataatc tgtcgattat tcaagggtac gatacgaaag ccagtcagtc ggcggtcttt
    20701 tatttcaggt cccaatgaaa aagtcgtata gcttgtcagc tcagataata tctcagcaga
    20761 ttgcttaata atctcattga tttcatgaaa ttctctgcct agagactgac gaatcagcag
    20821 caaatcatct tctgctacgc gagcaggttt taacaaatga tccacgtaat aacgatagcc
    20881 taaagcagat ggaatccgcc cagaagaaga gtgagtcttc aacagcaatc cttcatcttc
    20941 taatgctttc atatcattgc gaatcgttgc tgagctggcc ttgactccct cagacatcaa
    21001 tgttttggaa ccaacgggca cgccactact cgtatagtgt tggatgatca gacgtaaaat
    21061 gtcgttttgt cgctttgtta acatcttcct cacctctcat tagcactcat atattctaag
    21121 tgctaatcca cttataaata tagcaagaag cccttctcct gtcaagagaa agactccatt
    21181 ttttagcact ctaaacacaa gagtgctaat tctaaataat tgatgtaaat ccagttatat
    21241 caacgtttca atagtccttt attgtatttt tgaacttttt ttatttaaat tcagataaaa
    21301 aacaaatatt ctttaaacta tttttcaaac aaaaagcgct caaacacatt gttccccaaa
    21361 aaggtccctc ttggtgtcaa ataaacacgg tcttcttcat taacaagtaa cccttcttgc
    21421 tctaattccc ttagcacatc accgtacacc gattcaattg ttttttgata gcgatcatag
    21481 aaatgttttt tagtaatccc tgtttttttc ttagtcctaa gaacatttct tcttctattt
    21541 gattttttcg tgtcaatttt tcttcttcca ataccgggcg ttcatttgct cgtaatgggc
    21601 gtaaatagtg ttggattggt cctttgttgc ggtaacgaat atctccaaga taaccgctag
    21661 caccagctcc aaacccaaaa tagtggtcat tgttccagta catgaggtta tgttgagatt
    21721 catgtccgga gagcccaaag ttacttattt catattggtg acgtccatgc ttttccattg
    21781 cttcaatcgt ctcttcaaac attctggttt ccgtttcctg atcaggcagt tcgagtcttc
    21841 cttgtcgtac ccagttcatg aacattgttt tgttttcgag gatcaatgag tacaaagaat
    21901 agtgaggcag atccaaagcc aaagcttttt ccaaagtatc cagatatcct tctagtgttt
    21961 gtcccggtaa tgcataaatc aaatcgatgc ttacattggt aaaattttct gcttctaaaa
    22021 attgcatagt ttgatacaca tcttctgcgg tgtgtttacg tccaattttt ttcaataagc
    22081 gattatcaaa tgtctgcact cccatggata aacgattgac gccatagttt ttcattactt
    22141 gaagtttttc acgggtcaaa tcacctggat tcgcttctac agtaaactca ttaccatttt
    22201 gaaaaggcag gatctctcta gcacctgata acagtcgatc aagttgttta gcagataaag
    22261 aagttggtgt cccgccccct acgtaaagtg tttctaaatt cgctgtcgga tgactttcta
    22321 gcattatttt catttcattc agcagcattt ccacatactc atctactggc tgcccttcta
    22381 aaaaaacttt attaaaatca cagtaatagc agatatgctc gcaaaatggg atatgaatgt
    22441 acgcagaaac cttgttttct gcgggattta ttgtttctat cattgaaatt ctttctcctt
    22501 acgtcgaaaa gtcgtgcctg ttttcgacct tcttaaaatc gcgttcaagc accaactcat
    22561 tgaaataagc cgcatattta gaatcaagga aacctagctc taaaaatgct tgcttattgt
    22621 catcggagct acacggtgct tttacagcct ctcgataaac ggtaatcgca aagctgacct
    22681 tcggcattaa gtcaaaatct gcaaaaatat gaaaagctat tttcacagat tcctccttaa
    22741 gtgctcagac cacaacgctt tgcttacaac ctctcatata cgcatactaa acgtatttgg
    22801 cgaacaaatc cactaaaaaa tccaagaatc gacttcttgg atgatttttt ctttcattaa
    22861 actggttacc tagttatata tctctagggt tgttttcatg tctgagtgcc cgatatgatt
    22921 tatttttcgt ttctataatt actttttgcc ttccagtttc ttttaatatt gtactcaagc
    22981 aagtgggaat tggttctttt gtcatccatg tatgcaaaat gttataatgc tggtaaacaa
    23041 aaatgattac ctgtcatttt tatttagttc gtatttaatt aagttgacta tctgtatact
    23101 ctgtctaaaa cttaagaatt gttgttttcg ctgtcgctac ccaattttaa tgaatgtctg
    23161 taggttaaaa ctgttgtcct tgttatagat agtaaaggag agcttgaaaa tgaaaactgc
    23221 gattacgttt ctattagagg aatatgccga ttgggaaggt gcttatatta ctagcacact
    23281 aaaccaaaat gagaaatggc aaaacaaaac tgcttctatc caatcagagg tcacttccat
    23341 tggaggatta aaaacaaaaa tcgattactt aatcgaacaa atacctgaaa aagttgacct
    23401 ttttattatg atcggtggca attcatggga cataaaaaat catcatttat atcaaataat
    23461 cgataaattg ttaaaaacag acgtaccagt agctgctatt tgtggggcag ttgattattt
    23521 agctcagagc ggattattgc aaggttacaa acacaccgga aatgcgcaat ttccttggtt
    23581 agaatcggaa aattatcaga ataaagaaga ttttatagaa caacaagctg tgaaagatcg
    23641 taatttgatt actgcgaatg gaacagctcc tatagaattc acagacttga ttcttcaagc
    23701 agtccatttt gaaacagaag aaataagaga aaaaacgatt cgtctataca aacttggatt
    23761 ctatgattac tataaaaagt acggagatcc tttctcctaa tcattctctt caattaaata
    23821 ctccttatca atagtgtgac aagtaatttt actgatgatt atttgtcaca ctttttctga
    23881 catttatcaa agaagataca ctctatttat attcatagac aaggttttac ggattcaaaa
    23941 attcaggatt atgctatact ggtattaaaa aaataatgag gtattttata tgaaaaagtt
    24001 aggttttatt ttattgttgg tctctttatt ccttgtagga tgtacgtctc ctagtaaaga
    24061 aaatagtgat ccatctgcga ccacagacaa aagttcaatc agacaaacca gttcagctga
    24121 aaaatcaagt actcagaaat cagcttctac tgaaacgaat acatcttcaa caagcagtaa
    24181 agtctcagag cccaatactt cttctacatc tagtagtaca gttgagacaa atatttcttc
    24241 ttcaacaata gacacaacct cctcacagtc ttctcagtca atagatccgt tagcaggata
    24301 ttctgatcta caaattgagt acgcacgtgt ttggctagct attagaggta atacttttag
    24361 agatacatcg ggtgatttcg aattacatgt tattcattat cctgcaggaa cacctatcgc
    24421 cccttatgat gcagggagtg cagtctatcc gaaagatgtg gttatgctca ctgggaaata
    24481 cagttaccag ggagttttag tgtattcaag taaccatgac gggacaatca ctagttatcc
    24541 agttccttca cattggcaaa tgcctgcaga tcaaacatct tatccagcgt tcattagaga
    24601 aacaactcaa gaaatcattg ataatgcatc tgtagtagct attcctacag gaaatcccaa
    24661 tgacgtgaaa cagttaatcg atgtgacagt tatagataat tagactttct aatatgttat
    24721 acaaaaagaa gctgacagca ttttgtcagc ttctttcact ttcttttcta tttagattta
    24781 ttggtctttg atagactcca aggcaggaaa aaatttttta aataaacgtt tgcaaaacag
    24841 tcgcttttct atcttctacc gctacttctg cataggcacc attaatcaat gtcagttgtg
    24901 gcggtacatg accacaatca atatcataca agatcggtag ttttaattcc tttgacagat
    24961 cttcgtatac atccttcatt ttataatcta acaaatcctc tgccgcactt ctaccgaaca
    25021 tgatccccga gctgccatca aaccagcctg ccagtttcat ctgaacaagt gatcttctta
    25081 aatcaactga cgatagctca cagttttcta gaaaccacaa gatcggcttt ccatcattat
    25141 actttctttt aaaagattgg acatctccat aaggagtacc gattaaatgc ctgatcgtat
    25201 caatacaacc acctaataat cgtcccttca tccttactgc atctccgctg actgattgcc
    25261 aatttgttgg ttcagtgaga tggaaaacag aagatgatga atgctcatgg ggccacactt
    25321 tttgatactt ttcagaagat tgctgtagaa tcgattctcc ttctcttgtt ttcagtacac
    25381 tttcccacat tgctgtcgtt tcatccaagt actctcctct taaatcaata aggttagttc
    25441 catgtgcagt ggctatccct gtttttaatg tgacagcaag tagcaacgta ctaatatctg
    25501 aataacctac tatccatttt ggttgtagag catcaaaatc gacaaaatcg agaatctcaa
    25561 tcaataattc tcctccccaa gtcggaaaga tcatttggat attttcatct tgcatcattt
    25621 gattgaattc atctgctcgt tttcttgctg gacctgattt ttccttatct tgcgtccaaa
    25681 gcgtctttcc agtaactact tggaaagatt tttcttccat tctctttaaa gctgattcga
    25741 gtaacgaatg gtgttcctct gatacgcctg aggatggtgc agtaacccca atcgttgcat
    25801 ttctttctaa taatggataa cttatcatgt tgcttcacct ccaagtatgc actattttac
    25861 ttggttaatt gtttttttcg tttgccggca tcaaaaatat cggcatctca agtagctgga
    25921 tgattttgtt taagtcaacc gtttggatga ccatcgtttg atctggtcta cctggtgcaa
    25981 aaacaagttt ttcatttgat aagagctctt catcaataat aatcggaatc tgtttttcat
    26041 aaccaaacgg agaaacagcg ccctgctttt gtcccgtttg ttcttccatg cgttctgatg
    26101 aaacaataga aagcctttta ccgacgatct tccctaatgc tttgaaatcc gtttgtttcg
    26161 tagtaaaagt cagatatacg tagttattat cttttttatc ttttaagaat aagcttttgg
    26221 tctctgtacc tgtaaatcct tgttctttct ttattaccaa agcatcttca ttt
//
LOCUS       DAFGZE010000295        20985 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.050, whole genome shotgun sequence.
ACCESSION   DAFGZE010000295 DAFGZE010000000
VERSION     DAFGZE010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.050"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(134..577)
                     /locus_tag="LTU54_003297"
     CDS             complement(134..577)
                     /locus_tag="LTU54_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HBL8297902.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="LTU54_003298"
     CDS             complement(795..2135)
                     /locus_tag="LTU54_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8297903.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="LTU54_003299"
     CDS             2404..3300
                     /locus_tag="LTU54_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HBL8297904.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="LTU54_003300"
     CDS             complement(3450..4391)
                     /locus_tag="LTU54_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBL8297905.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="LTU54_003301"
     CDS             4517..5638
                     /locus_tag="LTU54_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HBL8297906.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="LTU54_003302"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="LTU54_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HBL8297907.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="LTU54_003303"
     CDS             7426..7992
                     /locus_tag="LTU54_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBL8297908.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="LTU54_003304"
     CDS             8203..9264
                     /locus_tag="LTU54_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBL8297909.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="LTU54_003305"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="LTU54_003305"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBL8297910.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="LTU54_003306"
     CDS             10177..12159
                     /locus_tag="LTU54_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBL8297911.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LTU54_003307"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="LTU54_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBL8297912.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="LTU54_003308"
     CDS             12881..14305
                     /locus_tag="LTU54_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBL8297913.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="LTU54_003309"
     CDS             complement(14486..15259)
                     /locus_tag="LTU54_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBL8297914.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="LTU54_003310"
     CDS             complement(15256..16161)
                     /locus_tag="LTU54_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBL8297915.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="LTU54_003311"
     CDS             complement(16315..16635)
                     /locus_tag="LTU54_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HBL8297916.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="LTU54_003312"
     CDS             17109..17795
                     /locus_tag="LTU54_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBL8297917.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="LTU54_003313"
     CDS             17797..18741
                     /locus_tag="LTU54_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBL8297918.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="LTU54_003314"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="LTU54_003314"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBL8297919.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="LTU54_003315"
     CDS             19663..20940
                     /locus_tag="LTU54_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBL8297920.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DAFGZE010000296        13213 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.079, whole genome shotgun sequence.
ACCESSION   DAFGZE010000296 DAFGZE010000000
VERSION     DAFGZE010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.079"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            complement(204..2162)
                     /locus_tag="LTU54_003316"
     CDS             complement(204..2162)
                     /locus_tag="LTU54_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBL8297921.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="LTU54_003317"
     CDS             complement(2159..3031)
                     /locus_tag="LTU54_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBL8297922.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="LTU54_003318"
     CDS             complement(3373..4245)
                     /locus_tag="LTU54_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBL8297923.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="LTU54_003319"
     CDS             4373..4942
                     /locus_tag="LTU54_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBL8297924.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="LTU54_003320"
     CDS             5009..5746
                     /locus_tag="LTU54_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBL8297925.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="LTU54_003321"
     CDS             5977..7245
                     /locus_tag="LTU54_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HBL8297926.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="LTU54_003322"
     CDS             7732..8196
                     /locus_tag="LTU54_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBL8297927.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="LTU54_003323"
     CDS             8180..8809
                     /locus_tag="LTU54_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBL8297928.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="LTU54_003324"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="LTU54_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HBL8297929.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="LTU54_003325"
     CDS             10688..11134
                     /locus_tag="LTU54_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HBL8297930.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="LTU54_003326"
     CDS             complement(11333..11848)
                     /locus_tag="LTU54_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBL8297931.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="LTU54_003327"
     CDS             11965..13167
                     /locus_tag="LTU54_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBL8297932.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DAFGZE010000297         8062 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.107, whole genome shotgun sequence.
ACCESSION   DAFGZE010000297 DAFGZE010000000
VERSION     DAFGZE010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.107"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            128..1564
                     /locus_tag="LTU54_003328"
     CDS             128..1564
                     /locus_tag="LTU54_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBL8297933.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="LTU54_003329"
     CDS             1753..4200
                     /locus_tag="LTU54_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBL8297934.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGESNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="LTU54_003330"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="LTU54_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBL8297935.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="LTU54_003331"
     CDS             complement(5690..6793)
                     /locus_tag="LTU54_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HBL8297936.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="LTU54_003332"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="LTU54_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HBL8297937.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2697 a         1374 c         1751 g         2240 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacataa ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaatcaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc
     7981 aacttatttc cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg
     8041 cttagtttcg agtagtaaga aa
//
LOCUS       DAFGZE010000298         2915 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.162, whole genome shotgun sequence.
ACCESSION   DAFGZE010000298 DAFGZE010000000
VERSION     DAFGZE010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.162"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            262..1476
                     /locus_tag="LTU54_003333"
     CDS             262..1476
                     /locus_tag="LTU54_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HBL8297938.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            1507..1725
                     /locus_tag="LTU54_003334"
                     /pseudo
     CDS             1507..1725
                     /locus_tag="LTU54_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2144..2617
                     /gene="dfrF"
                     /locus_tag="LTU54_003335"
     CDS             2144..2617
                     /gene="dfrF"
                     /locus_tag="LTU54_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HBL8297939.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            2744..>2915
                     /locus_tag="LTU54_003336"
     CDS             2744..>2915
                     /locus_tag="LTU54_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HBL8297940.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPF"
BASE COUNT          938 a          435 c          624 g          918 t
ORIGIN      
        1 atattttata tgaagtattc tttattcctt atgaaaaaat atctttaaaa ataaaaaaag
       61 acgaaaataa acggttttct ataaaaagaa gaccgtttat tttttgtctt tatttgtgtg
      121 aaaaacgaaa aagagcacaa cacatcaagc ttttattaat tgtctgtctt ttataaatgc
      181 aagtattaat tcaaaaaaac agaagaaaaa aacaactaca ccttctttta tactcgtaat
      241 cgtagaaagg aggcgaaaaa aatgaataca ttacctatca atacaggaga tacagcattc
      301 atgattttat gtacagcaat ggtttgcctg atgactcctg gtctagcttt tttttacggc
      361 ggattagcac ggaagaataa tatactaacg atcatgtctc aatcttttct ctccgttggg
      421 ataacaacga ttatgtgggt atttggtgga ttcggattgg cctttggacg ggatatcggc
      481 ggtgttattg gaaacccggc agatttcttt ttgatgagac atgttacaag cttccccaac
      541 atgtttcatg gtgcaacgat tccattttta atgttctttc tttaccaact gatgtttatc
      601 gtgattaccg ttcctttgat gacaggagct tttgcaggga gattgaattt aaaaggctat
      661 attatacttg ttattttttg gaatctgctg atttatttcc ctgtttgcca ctggatatgg
      721 ggtggcggat ttttggatca gatgggattt agagatttcg ctggtggagc agtcatccac
      781 acgacagctg gattcggctc tttagcatgt atcctcacac ttggtcaaag aaagattcct
      841 ttaggagaaa gaagtcagca tagcagtctt atggcagctg ctatcggtac aggactgctt
      901 tggtttggct ggttcggttt taattccggt ggtgcacttc gagcagatga acaagcagtc
      961 aatgcatttg gttcaacatt catcgctctt gcatttgcga tgattacttg gctgatcatt
     1021 gcaaaagtaa aaggaaatgg ctttgatttt gtcgacgtac taacaggatc cgttgctgga
     1081 ttggcagcca ttacgccttg tgccggttat gtagaagcaa aaagtgccat gctgattggg
     1141 atcattgcgg gtatcgtctg tcatgctgct gttgattttc gtaaaaagaa acaatgggat
     1201 gatgcacttg atgtgtgggg tgttcatggc atgggcggat tcacaggtac gattctgata
     1261 gggatctttg catctaacag ccatttacta acgaatccaa gcagctggta cttcttaggg
     1321 attcaagtga ttggtgtcat cattactgcc gtgtatgcct atgtgctgac aacagtgatt
     1381 ttaaaatcgg cgatgcactt tacaacgatc actactacaa aagaagaaca agaagaagga
     1441 ttggactttt tcttctttgg caacaaactc aattgaaaca aattcaaata gaaaagagga
     1501 agattcatgc aacaaagcaa aacgaaaatt cctgcaaaaa aattaacgtt ctttggcttt
     1561 ttggctatga ccatctcaat ggtcgtttca ttatatgaat atcccacatt cgcgacctca
     1621 ggcttcagct tagtattttt cttgttatta ggcggtttgc tatggtttat ccctgtggca
     1681 ttgtgcgcag cagagatggc aacggtgaaa ggatgggaaa aaggtgtttc ccaaacaaaa
     1741 agatattaag aaacagccca tgtttacctg cttgcaaaca aaagaatatt gctactagac
     1801 taagagatac tacataagtg tgttggtatc tcttttttgt tgccgtgaaa cagcagacta
     1861 tttcatttct gcttgattac cttaaaactt attagtctat ggtttgtcaa gggcttgttg
     1921 cgtgagaaat gctgaatatc accctttaca aaacataggc tgataagtaa cttttcaaaa
     1981 gcagaaaaga aatggcaatg cttataacaa tatgatataa tgcagatgaa gacaaaaaag
     2041 gaatttgtcg agctggttag ttcgagataa ttttgagttt gttaagatgt ggtgcacttg
     2101 atggaatcac aggtacgaca tcatattatg gaggatgaaa tagatgatag gtttgattgt
     2161 tgcgaggtca aagaataatg ttataggcaa gaatggtaat ataccatgga aaataaaggg
     2221 agaacaaaag caatttagag agttaacaac gggtaatgtg gttattatgg ggcgaaagtc
     2281 ttatgaagaa atcggtcatc cgttgcctaa tagaatgaat attgttgttt ccaccacaac
     2341 agagtatcaa ggagataatt tagtttcagt taaatcatta gaagatgcat tattattggc
     2401 taaaggacga gatgtataca tatctggtgg atatggacta tttaaggaag ctttgcaaat
     2461 agtagataaa atgtatatca cagaagtaga tttaaatatt gaagatggag atacattctt
     2521 tccagaattt gatatcaatg attttgaagt tttgataggg gaaacacttg gtgaggaagt
     2581 gaaatatacg agaacatttt atgtaaggaa aaaatgaatt gagtaaattt tggatttagg
     2641 gtgttttcat aaatatattg ctttttatgc atatataaat tgttgtaaga caaattccag
     2701 tttgtatggg gcgataaaat cagaatttga gagaggattg aatatgaata aggaatggtc
     2761 tgaacttaat aaaacaatgc aggcacaaat aaaaaagaaa gatacttata agaggggtat
     2821 tgatacttta cttactttac gaagtcagtt aatacaaacc ttagtatctt tcaaagaaga
     2881 attatgcaga gaagatttta actcaatccc cttca
//
LOCUS       DAFGZE010000299         1571 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.200, whole genome shotgun sequence.
ACCESSION   DAFGZE010000299 DAFGZE010000000
VERSION     DAFGZE010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.200"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            266..1141
                     /locus_tag="LTU54_003337"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="LTU54_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HBL8297941.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1271..>1571)
                     /locus_tag="LTU54_003338"
     CDS             complement(1271..>1571)
                     /locus_tag="LTU54_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBL8297942.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          622 a          150 c          265 g          534 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataat aaaggaagtg agtcaattcc agactcctgt gtaaaatgct atacaatgtt
     1261 tttaccattt ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat
     1321 gtaggtccat aagaacggct ccaattaagc gattggctga tgtttgattg gggaagatgc
     1381 gaataatctt ttctcttctg cgtacttctt gattcagtcg ttcaattaga ttggtactct
     1441 ttagtcgatt gtgggaattt ccttgtacgg tatattgaaa ggcgtcttcg aatccatcat
     1501 ccaatgatgc gcaagctttt gaatattttg gttggttctg ttgcaaagtt ttaaatctac
     1561 tatcaaataa g
//
LOCUS       DAFGZE010000300         1504 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.204, whole genome shotgun sequence.
ACCESSION   DAFGZE010000300 DAFGZE010000000
VERSION     DAFGZE010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.204"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            262..345
                     /locus_tag="LTU54_003339"
                     /pseudo
     CDS             262..345
                     /locus_tag="LTU54_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="LTU54_003340"
     CDS             394..477
                     /locus_tag="LTU54_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HBL8297943.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="LTU54_003341"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="LTU54_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBL8297944.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DAFGZE010000301         1464 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.208, whole genome shotgun sequence.
ACCESSION   DAFGZE010000301 DAFGZE010000000
VERSION     DAFGZE010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.208"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            40..258
                     /locus_tag="LTU54_003342"
                     /pseudo
     CDS             40..258
                     /locus_tag="LTU54_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            291..1199
                     /locus_tag="LTU54_003343"
     CDS             291..1199
                     /locus_tag="LTU54_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HBL8297945.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1196..1444
                     /locus_tag="LTU54_003344"
     CDS             1196..1444
                     /locus_tag="LTU54_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HBL8297946.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT          523 a          207 c          322 g          412 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaacctt attatgaggg caaacggaca
       61 gctatgtttt tggaagaaaa ggttacaaaa tatcatagaa cactgaccac atatctaaat
      121 acactgcttt caaatagttt tataataaat cagattgtgg agccacagcc gccagagaac
      181 atgatggata ttccggggat ggcggatgaa atgcgacgcc caatgatgct gattgtatcg
      241 gcaaaaaaga agatgtaata atatagaaaa aataaacgag gagtatgtaa atgagatcag
      301 aaaaagaaat gatggattta gtactttctt tagcagaaca ggatgaacgt attcgaattg
      361 tgacccttga ggggtcacgc gcaaatatta atatacctaa agatgaattt caggattatg
      421 atattacata ttttgtaagt gatatagaac cgtttatatc taatgatgac tggcttaatc
      481 aatttgggaa tataataatg atgcaaaagc cggaggatat ggaattattc ccacctgaag
      541 aaaagggatt ttcctatctt atgctatttg atgattacaa taaaattgat cttaccttat
      601 tgcccttgga agagttagat aattacctaa agggcgataa attaataaag gttctaattg
      661 ataaagattg tagaattaaa agggacatag ttccgactga tatagattat catgtaagaa
      721 agccaagcgc aagggagtat gatgattgct gcaatgaatt ttggaatgta acaccttatg
      781 ttattaaagg attgtgccgt aaggaaattt tatttgctat tgatcatttt aatcagattg
      841 ttcgccatga gctgctgaga atgatatcat ggaaggtcgg catcgaaaca ggctttaaat
      901 taagtgtagg caagaactat aagtttattg aaaggtatat atccgaggat ttgtgggaga
      961 aacttttgtc cacctaccgg atggattcct atgaaaacat atgggaagca ttatttctat
     1021 gccatcaatt gttcagggcg gtatccggtg aggtggcgga aaggcttcat tatgcctatc
     1081 cggagtatga taggaatata acaaaatata ccagggacat gtataaaaaa tacactggta
     1141 aaaccggctg cctggatagc acatatgccg ctgatataga agagaggcgg gaacagtgat
     1201 tacagaaatg aaagcagggc acctgaaaga tatcgataaa cccagcgaac catttgaggt
     1261 gataggtaag attataccga ggtatgaaaa cgagaattgg acctttacag aattactcta
     1321 tgaagcgcca tatttaaaaa gctaccaaga cgaagaggat gaagaggatg aggaggcaga
     1381 ttgccttgaa tatattgaca atactgataa gataatatat cttttaaccc gtggtgctag
     1441 ttaattttaa tatcctaaag aata
//
LOCUS       DAFGZE010000302         1349 bp    DNA     linear   BCT 07-DEC-2021
DEFINITION  TPA_asm: Enterococcus faecium isolate DMG1901751
            SAMEA9566056-rid13446853.guided.218, whole genome shotgun sequence.
ACCESSION   DAFGZE010000302 DAFGZE010000000
VERSION     DAFGZE010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA9566056
            Sequence Read Archive: ERR6615690
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (03-DEC-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 03-DEC-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001189671.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001189671.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/03/2021 22:33:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,346
            CDSs (total)                      :: 3,283
            Genes (coding)                    :: 3,200
            CDSs (with protein)               :: 3,200
            Genes (RNA)                       :: 63
            rRNAs                             :: 1, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 83
            CDSs (without protein)            :: 83
            Pseudo Genes (ambiguous residues) :: 0 of 83
            Pseudo Genes (frameshifted)       :: 37 of 83
            Pseudo Genes (incomplete)         :: 39 of 83
            Pseudo Genes (internal stop)      :: 20 of 83
            Pseudo Genes (multiple problems)  :: 12 of 83
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA9566056-rid13446853.guided.218"
                     /isolate="DMG1901751"
                     /isolation_source="Human sample"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /collection_date="2013"
     gene            46..297
                     /locus_tag="LTU54_003345"
                     /pseudo
     CDS             46..297
                     /locus_tag="LTU54_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="LTU54_003346"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="LTU54_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HBL8297947.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
